LOCUS       DAZOZD010000001        54228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOZD010000001 DAZOZD010000000
VERSION     DAZOZD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.001"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(379..465)
                     /locus_tag="ACHUPB_000001"
     tRNA            complement(379..465)
                     /locus_tag="ACHUPB_000001"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(429..431),aa:Leu,seq:gag)
     gene            complement(480..812)
                     /gene="secG"
                     /locus_tag="ACHUPB_000002"
     CDS             complement(480..812)
                     /gene="secG"
                     /locus_tag="ACHUPB_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0756937.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1040..2377)
                     /gene="glmM"
                     /locus_tag="ACHUPB_000003"
     CDS             complement(1040..2377)
                     /gene="glmM"
                     /locus_tag="ACHUPB_000003"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0756938.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2370..3218)
                     /gene="folP"
                     /locus_tag="ACHUPB_000004"
     CDS             complement(2370..3218)
                     /gene="folP"
                     /locus_tag="ACHUPB_000004"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0756939.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3308..5242)
                     /gene="ftsH"
                     /locus_tag="ACHUPB_000005"
     CDS             complement(3308..5242)
                     /gene="ftsH"
                     /locus_tag="ACHUPB_000005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0756940.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5342..5971)
                     /gene="rlmE"
                     /locus_tag="ACHUPB_000006"
     CDS             complement(5342..5971)
                     /gene="rlmE"
                     /locus_tag="ACHUPB_000006"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0756941.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6097..6390
                     /gene="yhbY"
                     /locus_tag="ACHUPB_000007"
     CDS             6097..6390
                     /gene="yhbY"
                     /locus_tag="ACHUPB_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0756942.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6546..7022)
                     /gene="greA"
                     /locus_tag="ACHUPB_000008"
     CDS             complement(6546..7022)
                     /gene="greA"
                     /locus_tag="ACHUPB_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0756943.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7270..8703
                     /gene="dacB"
                     /locus_tag="ACHUPB_000009"
     CDS             7270..8703
                     /gene="dacB"
                     /locus_tag="ACHUPB_000009"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0756944.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            8758..8865
                     /locus_tag="ACHUPB_000010"
                     /pseudo
     CDS             8758..8865
                     /locus_tag="ACHUPB_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8889..10061)
                     /gene="cgtA"
                     /locus_tag="ACHUPB_000011"
     CDS             complement(8889..10061)
                     /gene="cgtA"
                     /locus_tag="ACHUPB_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0756945.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10077..11042)
                     /locus_tag="ACHUPB_000012"
     CDS             complement(10077..11042)
                     /locus_tag="ACHUPB_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0756946.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11169..11426)
                     /gene="rpmA"
                     /locus_tag="ACHUPB_000013"
     CDS             complement(11169..11426)
                     /gene="rpmA"
                     /locus_tag="ACHUPB_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0756947.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11447..11758)
                     /gene="rplU"
                     /locus_tag="ACHUPB_000014"
     CDS             complement(11447..11758)
                     /gene="rplU"
                     /locus_tag="ACHUPB_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0756948.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12017..12988
                     /gene="ispB"
                     /locus_tag="ACHUPB_000015"
     CDS             12017..12988
                     /gene="ispB"
                     /locus_tag="ACHUPB_000015"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0756949.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13217..13495
                     /gene="sfsB"
                     /locus_tag="ACHUPB_000016"
     CDS             13217..13495
                     /gene="sfsB"
                     /locus_tag="ACHUPB_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0756950.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13543..14802)
                     /gene="murA"
                     /locus_tag="ACHUPB_000017"
     CDS             complement(13543..14802)
                     /gene="murA"
                     /locus_tag="ACHUPB_000017"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0756951.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14857..15111)
                     /gene="ibaG"
                     /locus_tag="ACHUPB_000018"
     CDS             complement(14857..15111)
                     /gene="ibaG"
                     /locus_tag="ACHUPB_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0756952.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15271..15564)
                     /gene="mlaB"
                     /locus_tag="ACHUPB_000019"
     CDS             complement(15271..15564)
                     /gene="mlaB"
                     /locus_tag="ACHUPB_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0756953.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15564..16199)
                     /gene="mlaC"
                     /locus_tag="ACHUPB_000020"
     CDS             complement(15564..16199)
                     /gene="mlaC"
                     /locus_tag="ACHUPB_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0756954.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16218..16769)
                     /gene="mlaD"
                     /locus_tag="ACHUPB_000021"
     CDS             complement(16218..16769)
                     /gene="mlaD"
                     /locus_tag="ACHUPB_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0756955.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16774..17556)
                     /gene="mlaE"
                     /locus_tag="ACHUPB_000022"
     CDS             complement(16774..17556)
                     /gene="mlaE"
                     /locus_tag="ACHUPB_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0756956.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17564..18373)
                     /gene="mlaF"
                     /locus_tag="ACHUPB_000023"
     CDS             complement(17564..18373)
                     /gene="mlaF"
                     /locus_tag="ACHUPB_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0756957.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18583..19560
                     /locus_tag="ACHUPB_000024"
     CDS             18583..19560
                     /locus_tag="ACHUPB_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0756958.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19574..20560
                     /gene="kdsD"
                     /locus_tag="ACHUPB_000025"
     CDS             19574..20560
                     /gene="kdsD"
                     /locus_tag="ACHUPB_000025"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0756959.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20581..21147
                     /gene="kdsC"
                     /locus_tag="ACHUPB_000026"
     CDS             20581..21147
                     /gene="kdsC"
                     /locus_tag="ACHUPB_000026"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0756960.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21144..21719
                     /gene="lptC"
                     /locus_tag="ACHUPB_000027"
     CDS             21144..21719
                     /gene="lptC"
                     /locus_tag="ACHUPB_000027"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0756961.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21688..22245
                     /gene="lptA"
                     /locus_tag="ACHUPB_000028"
     CDS             21688..22245
                     /gene="lptA"
                     /locus_tag="ACHUPB_000028"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0756962.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22252..22977
                     /gene="lptB"
                     /locus_tag="ACHUPB_000029"
     CDS             22252..22977
                     /gene="lptB"
                     /locus_tag="ACHUPB_000029"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0756963.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23025..24458
                     /gene="rpoN"
                     /locus_tag="ACHUPB_000030"
     CDS             23025..24458
                     /gene="rpoN"
                     /locus_tag="ACHUPB_000030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0756964.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24481..24768
                     /gene="hpf"
                     /locus_tag="ACHUPB_000031"
     CDS             24481..24768
                     /gene="hpf"
                     /locus_tag="ACHUPB_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0756965.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24886..25377
                     /gene="ptsN"
                     /locus_tag="ACHUPB_000032"
     CDS             24886..25377
                     /gene="ptsN"
                     /locus_tag="ACHUPB_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0756966.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25423..26277
                     /gene="rapZ"
                     /locus_tag="ACHUPB_000033"
     CDS             25423..26277
                     /gene="rapZ"
                     /locus_tag="ACHUPB_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0756967.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26274..26546
                     /gene="npr"
                     /locus_tag="ACHUPB_000034"
     CDS             26274..26546
                     /gene="npr"
                     /locus_tag="ACHUPB_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0756968.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26760..27392
                     /gene="yrbL"
                     /locus_tag="ACHUPB_000035"
     CDS             26760..27392
                     /gene="yrbL"
                     /locus_tag="ACHUPB_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0756969.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27389..28117)
                     /gene="mtgA"
                     /locus_tag="ACHUPB_000036"
     CDS             complement(27389..28117)
                     /gene="mtgA"
                     /locus_tag="ACHUPB_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR0756970.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28114..28767)
                     /gene="elbB"
                     /locus_tag="ACHUPB_000037"
     CDS             complement(28114..28767)
                     /gene="elbB"
                     /locus_tag="ACHUPB_000037"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0756971.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28997..31333)
                     /gene="arcB"
                     /locus_tag="ACHUPB_000038"
     CDS             complement(28997..31333)
                     /gene="arcB"
                     /locus_tag="ACHUPB_000038"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0756972.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31429..32358)
                     /locus_tag="ACHUPB_000039"
     CDS             complement(31429..32358)
                     /locus_tag="ACHUPB_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0756973.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTFTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33033..37493
                     /gene="gltB"
                     /locus_tag="ACHUPB_000040"
     CDS             33033..37493
                     /gene="gltB"
                     /locus_tag="ACHUPB_000040"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0756974.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37506..38924
                     /gene="gltD"
                     /locus_tag="ACHUPB_000041"
     CDS             37506..38924
                     /gene="gltD"
                     /locus_tag="ACHUPB_000041"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0756975.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39108..39512
                     /locus_tag="ACHUPB_000042"
                     /pseudo
     CDS             39108..39512
                     /locus_tag="ACHUPB_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            39604..39909
                     /locus_tag="ACHUPB_000043"
     CDS             39604..39909
                     /locus_tag="ACHUPB_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0756976.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(39985..40449)
                     /gene="nanQ"
                     /locus_tag="ACHUPB_000044"
     CDS             complement(39985..40449)
                     /gene="nanQ"
                     /locus_tag="ACHUPB_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0756977.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40446..41321)
                     /gene="nanK"
                     /locus_tag="ACHUPB_000045"
     CDS             complement(40446..41321)
                     /gene="nanK"
                     /locus_tag="ACHUPB_000045"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0756978.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41318..42007)
                     /locus_tag="ACHUPB_000046"
     CDS             complement(41318..42007)
                     /locus_tag="ACHUPB_000046"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0756979.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42055..43545)
                     /gene="nanT"
                     /locus_tag="ACHUPB_000047"
     CDS             complement(42055..43545)
                     /gene="nanT"
                     /locus_tag="ACHUPB_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0756980.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43655..44548)
                     /gene="nanA"
                     /locus_tag="ACHUPB_000048"
     CDS             complement(43655..44548)
                     /gene="nanA"
                     /locus_tag="ACHUPB_000048"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0756981.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44670..45461)
                     /gene="nanR"
                     /locus_tag="ACHUPB_000049"
     CDS             complement(44670..45461)
                     /gene="nanR"
                     /locus_tag="ACHUPB_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0756982.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            45841..47163
                     /gene="dcuC"
                     /locus_tag="ACHUPB_000050"
     CDS             45841..47163
                     /gene="dcuC"
                     /locus_tag="ACHUPB_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0756983.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(47206..47703)
                     /gene="sspB"
                     /locus_tag="ACHUPB_000051"
     CDS             complement(47206..47703)
                     /gene="sspB"
                     /locus_tag="ACHUPB_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0756984.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47709..48347)
                     /gene="sspA"
                     /locus_tag="ACHUPB_000052"
     CDS             complement(47709..48347)
                     /gene="sspA"
                     /locus_tag="ACHUPB_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0756985.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(48742..49134)
                     /gene="rpsI"
                     /locus_tag="ACHUPB_000053"
     CDS             complement(48742..49134)
                     /gene="rpsI"
                     /locus_tag="ACHUPB_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0756986.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(49150..49578)
                     /gene="rplM"
                     /locus_tag="ACHUPB_000054"
     CDS             complement(49150..49578)
                     /gene="rplM"
                     /locus_tag="ACHUPB_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0756987.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(49797..50924)
                     /gene="zapE"
                     /locus_tag="ACHUPB_000055"
     CDS             complement(49797..50924)
                     /gene="zapE"
                     /locus_tag="ACHUPB_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0756988.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            51118..51516
                     /gene="zapG"
                     /locus_tag="ACHUPB_000056"
     CDS             51118..51516
                     /gene="zapG"
                     /locus_tag="ACHUPB_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0756989.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            51670..53037
                     /gene="degQ"
                     /locus_tag="ACHUPB_000057"
     CDS             51670..53037
                     /gene="degQ"
                     /locus_tag="ACHUPB_000057"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR0756990.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            53127..54194
                     /gene="degS"
                     /locus_tag="ACHUPB_000058"
     CDS             53127..54194
                     /gene="degS"
                     /locus_tag="ACHUPB_000058"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0756991.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        13344 a        14567 c        13522 g        12795 t
ORIGIN      
        1 tccatatcag accgaaacac tgttagtttt ttctttttaa atgatgcggc cattgcgttc
       61 tcagtaatta aataacatta tcaatgcgtc tctaaacatt acgcagaacg tagccatcag
      121 tagttatact gacaggagca ggatggttta tcgagaagta aacatatccg tattcgtacc
      181 ggaactggag ttgacacaat aaagtgccaa ttatgtcagc agaagggaaa agtttataac
      241 taaggcgtac cggcaccatc gtttcaaggt accagctacg agtaaagcaa ctggacgaga
      301 tacagatacc tgacaaccat tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag
      361 cgtctctttt ctggaatttg gtaccgagga cgggacttga acccgtaagc cctattgggc
      421 actaccacct caaggtagcg tgtctaccaa ttccaccacc tcggcacgga tactactttt
      481 tagttcggga tatcgctggt cggcttagcc ggagcagctg gctgagtttg ttcggttttc
      541 gccggtgcac tcagattttc ccattcgcta cctttattgg ttttgttgct attgatgtta
      601 cccagcacca gactgatgat aaagaataac gttgccagca gcgccgtcat gcgggtcatg
      661 aagttaccag aaccacttga accaaacagc gtagcggaag cgcctgctcc gaaggaggct
      721 cccatatcag cgcctttacc ttgctgcagc atgatcagac caacaaggcc aattgccaca
      781 ataaggaaaa ctaccaaaag agcttcgtac ataatcaacc tgttccttgc ggagttgccg
      841 cgtaccaatg cttcaaccaa taaagcggga gtttttaatc tttcccactg aagtgggtgt
      901 gaatactaac caaagcgaat gaccttcgca agggcaattt tatcgcattg tatcaactgc
      961 ggaaaaaaac agcaaaaccg atgtgttcgc tgagaaaaaa ggcgacaatc gccgcctttt
     1021 tagccagtta tttaccgttt taaacggctt ttactgcatc ggcgatacgg tgtgcaaatt
     1081 cagttacctg cgcttcgtct tcaccttcta ccatcacgcg aattaacggt tcggtgccgg
     1141 atttacgcaa cagcacacgt ccacggctac ccagcgccgc ttcaacttcg gcggtcacag
     1201 ctttaactga ctcatgctca agcggatcgc cgctacctgc ggtgtaacgc acgttaacca
     1261 gaatctgcgg gaacattttc atgccgctgc aaaggtcgtg caggctcata tggttacgtg
     1321 ccatcgccgc cagcacctgc aagccagcaa cgatgccgtc accggtagtg gttttatcca
     1381 gcaggatcac atgaccggaa ttctctgcac cgatacgcca gcctttctcc tgcatttttt
     1441 ccagtacgta gcggtcaccc actttcgcgc gcgcaaatgg aattcctaac tgtttcaacg
     1501 ccagctcaag ccccatgtta ctcatcaatg tacccacagc accaccacgc agctggccct
     1561 gacgaagacc ttcacgcgcg atgatataca tgatctgatc accatcgact ttattgcctt
     1621 catggtcaac cataatcacg cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt
     1681 tttcagccag cacacgagcc tggagcgcgc gaacgtcggt agcccccact tcggcattga
     1741 tgtttacacc gttcggctca caaccgatag cgataacgtt cgcccccagt tcacgcagca
     1801 cgttcggcgc gatgtgataa gtcgcaccgt ttgcgcaatc caccacaatc ttcagttcgc
     1861 tgaggctaag ttcgttcggg aacgtggctt tgcaaaactc gatatagcga cccgcagcat
     1921 caacgatacg gctggcttta cccagttctg ccgaatcaac acagctgatc tctttttcca
     1981 tttccgcttc gatggcctct tctaccgcat ccggtagttt ggtgccgtcg atagagaaaa
     2041 atttaatgcc attatcgtag aacgggttat gcgatgcaga tatcacaatt ccggcctctg
     2101 cgcggaaggt acgcgtcaga taagccacgg ccggtgttgg catcgggcca gtgaagagtg
     2161 cggaaaggcc cgctgccgcc agacccgctt ccagtgctga ctccagcata tagccagaaa
     2221 tacgcgtgtc tttaccaata atgatcttac gggagccgtg gcgcgccagc actttacccg
     2281 cggcccaacc cagcttaagc acaaaatcag gtgtgatcgg cgcatcccct acacgaccac
     2341 gaatcccatc ggtaccgaaa tatttacgat tactcatagc gtttgttttc ctttgcagac
     2401 agagtggctt ccaccacccg catcgcttct acggtttctt tgacgtcatg aacacgaata
     2461 atgtgcgcgc cttgcattgc ggcaatgacc gcacaggcca gactaccgct caggcgctcg
     2521 gacggcccca cgttcagcag ctgcccaatc atcgattttc gtgacatacc taccaacagc
     2581 ggcaggttga aatggtgaaa ttcagccagg cgcgccagta atgaatagtt atgggagaga
     2641 tttttaccga aaccgaatcc ggggtcgagc aacaatttct cttttgcgat acccgcctgc
     2701 tcgcaacgtg ctatttgctc aataaagtag cgattcactt ctgcaaagac atcgtcatac
     2761 ttcggagctt cctgcatggt ttttggattt ccctgcatat gcatcagaca aaccggtaaa
     2821 ccggtttctg cagccgcctc cagagcgcca ggttcggaaa gggagcggat atcgttaata
     2881 atgtgagcgc caactttcgc tgactcacgg atgacttctg gtttggatgt atctaccgag
     2941 atccagactt cgaagcgttg agcaattgcc tcaaccacag gaataacacg ttgcaactct
     3001 tcttcaacgc taacttccgc cgcccctggg cgcgtggact cgccaccaac gtcaatgatc
     3061 gtcgcgccag cgttgatcat cagattcgca tgtttcaccg catctatcag cgagttatgc
     3121 gtgccaccat ccgaaaagga atcaggcgtg acgttgagga tccccattac gtgaggatgg
     3181 ctaaggtcca gtgaagtgcc ctgggcaaag agtttcatgg tgttatccct ggtatgaatt
     3241 gataagaaaa aaccccggag cacgccccgg ggttttcggt acaaatacag tcatctgatg
     3301 cgggaactta cttgtcgcct aactgctctg acatggtgtt acccgggttc ggcgtacgcg
     3361 gttcatcaac cggacgagga gcctttggac taccattgtc gccagaattg ttagaagcgc
     3421 ctggttcttc ccagcccgct ggcggacgta catcgcgacg tgccatcagg tcatcaatct
     3481 gcggtgcgtc gatagtctca tatttcatga gagcatcttt catcgcatgc agaatatcca
     3541 tattgtcggt cagaagctga cgcgcacgat tatagttacg ctcaatcagt gctttcactt
     3601 cctggtcgat gatacgtgca gtttcatcgg acatatgttt cgctttcgct acgctacggc
     3661 cgaggaacac ttcaccttct tcttccgcgt acagcagtgg acccaatttc tcagagaagc
     3721 cccactgagt caccatgtta cgcgccaggt tggtcgcaac tttaatatcg ttggacgcac
     3781 cggtagaaac atgttccggc ccgtagatga tctcttctgc cagacgacca ccgtacagcg
     3841 tagaaatctg gctttccagt ttctgacggc tggcgctgat tgcgtcgccc tcaggcaaga
     3901 agaaagtcac acccagcgca cgaccgcgtg ggataatcgt cactttgtgc accggatcgt
     3961 gttccggcac caggcgaccg ataatcgcat gacccgcttc gtggtaagcc gtcgattctt
     4021 tctgcgcttc cgtcatcacc atggagcgac gttccgcacc catcatgatt ttatctttcg
     4081 ctttctcgaa ctcaaccatc gacacaacgc gtttgttgcc acgagcagcg aacagtgccg
     4141 cttcgtttac caggttcgcc aggtcagcac cggagaaacc aggagtacca cgggcaatga
     4201 ttgccgcgtc gatatcgggt gccaatggta cgcgacgcat gtgaactttc aggatctgct
     4261 cacgaccgcg aacatctggc aagccaacca caacctgacg gtcgaaacgg ccaggacgca
     4321 gcagggccgg gtcaagaacg tccggacggt tagtcgcggc gataacgatg ataccttcgt
     4381 taccttcgaa gccatccatc tcaaccagca tctggttcag agtctgttca cgttcatcgt
     4441 gaccaccgcc cagaccagcg ccacgctggc ggcctacggc gtcgatttca tcgataaaga
     4501 tgatgcacgg tgccgctttc ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca
     4561 caccgacgaa catttctacg aagtcagaac cggagatagt aaagaacgga actttcgctt
     4621 cgcctgcaat cgctttcgcc agcagcgttt taccggtacc cggaggaccg accatcagga
     4681 cgcctttcgg gatcttaccg ccgagtttct ggaagcggct cggctcgcgc agatattcaa
     4741 ccagttcagc aacttcttct tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt
     4801 tgatctgatc ttccgtcagc atgcgcgctt tgctcttacc aaacgacatg gcacctttgc
     4861 caccgccgcc ctgcatttga cgcatgaaga agatccacac accaatcagc aacagcatcg
     4921 ggaaccagga gatgaagata gaagccagca ggcttggttc ttcaggcggt tcaccgacaa
     4981 ccttgacgtt cttggtcaac aggttatcca gtaatttcgg atcctgaacc ggaatgtaag
     5041 tggtataacg gttactatct ttcttggtaa cgttgatttc acgtccgttg atacgcgctt
     5101 cacgaacctg gtcgttattg acctcttgta ggaaggtaga gtaatccacc ttacggccat
     5161 tagactcgct gggcccaaag ctctggaata ctgacatcag cacaacggca atgaccagcc
     5221 agagtattag gtttttcgcc atgtcactca agggattaac ctcttattac aactgtgtta
     5281 aaaacagcgt caggatactc tatatccagc atctttcaaa ctttcgtctg aaatctcccg
     5341 gttagggttt acgcccggtc gctacaatat acacttcccg cgaacgtgca cgagaagagt
     5401 ccggcttacg aactttgacc ttcgtaaaca gggagcgaat ttcccttaga tactcatcga
     5461 aaccttcgcc ctggaacacc ttcactacaa aactgccacc tggcgctaat acatcacgac
     5521 acatttctag cgccagttcc accagataca tggcacgggg gatatccacc gccggtgttc
     5581 cgctcatgtt tggtgccata tcggacatga caacctggac tttgctgtcg ccaacgcgct
     5641 ccagcagtgc tttcatcacc agttcatcac gaaaatcgcc ctgaagaaag tctacaccaa
     5701 cgataggatc cataggtaaa agatcgcaag cgatgatgcg gcctttgccg ccaatttggg
     5761 tgaccacata ttgtgaccaa ccacccggag cagcaccaag gtcgacaacc gtcattcccg
     5821 gtttaaagag tttgtcactt tgctgtattt catcaagttt aaaccaggca cgggaacgta
     5881 accccttttt ctgtgcctgt tgaacatatt tatcgctaaa gtgttcctga agccagcggc
     5941 tggagctggc agaacgcttc ttacctgtca tttaactttc ccatggggat aactcatcgt
     6001 aaccaattgc gtaaattttt acgcgcctat ttggtgatat atgggagatg gcggtagaat
     6061 gacccatttt caatcccaac gtaagcaaaa aatacgatga atctgagtac taaacaaaaa
     6121 cagcacctga aaggtctggc acatccgctc aagccagttg ttctgcttgg cagtaatggt
     6181 ttgaccgaag gggtgctggc cgagattgaa caagcgttag agcaccatga actcatcaag
     6241 gtgaaaatcg ccaccgaaga tcgcgaaaca aaaaccttga tcgtggaagc tatcgtgcgc
     6301 gaaaccggcg cctgtaatgt acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact
     6361 aaagaacgta aaatctcgct gccacgctaa gattatccta aagttacaca catttgctgt
     6421 gtaaaacgag gggttttccg caggcaggag agcaaaatgc cacgctctgt tcgttgataa
     6481 aaggccgcat agcggccttt ttcctttctt tacaatacat caacatcttg agtattgggt
     6541 aattcttaca ggtattccac cttaattact tcaaattcta cttcgccgcc cggcgttttg
     6601 atgaccacaa catcatcttc ttctttgccg atcaggccac gagcaatagg cgagtttaca
     6661 gaaatcaggt tttgtttaaa gtcagcttcg tcatcgccaa cgatgcgata agtctgttct
     6721 tcgtcagaat ccagattcag caccgttacg gtagcaccaa aaataacgcg cccattgtta
     6781 ggcattttgg tgacatcaat cacctgcgcg tttgacagct tggcttcgat gtctttgata
     6841 cggccttcgc agaagccctg ctgttcacga gctgcgtggt attcggcgtt ttctttcagg
     6901 tcgccatgct cacgcgcttc cgcgatagca gcaatgattt caggacggcg cacagatttc
     6961 agaaaatcca gctcttcgcg taatttttca gcgccgcgta aggtcatcgg aatagcttgc
     7021 atttgttata cctcttgaat attcctgatg gggcaagtct tcacccaccc cggctgttcg
     7081 gcctgcccgg cataactcct caccgggacc agaagcaaaa aaataccgac ccaggtacaa
     7141 gtcccaggtc agctacaatt cacattttga tagtcatttt accctgaagt tcccgaaggg
     7201 tcatcgttta ctttataggg cgttgcgccg tagtatgacg gctcgattcc aggttgttag
     7261 cgcgagatta tgcgattttc cagatttatc atcggattga ccagctgtat agcgttcagt
     7321 gttcaggccg caaatgttga tgagtacatt actcaactcc ccgctggtgc caaccttgcc
     7381 ctgatggtgc aaaaagtcgg cgcgtcggcc cccgctattg attaccacag tcagcagatg
     7441 gcgctgcctg ccagtaccca gaaagtgatt actgcgctgg cggcgttgat tcaactcggc
     7501 cccgattttc gttttaccac gacgcttgaa accaaaggca atgtggaaaa cggcgtactt
     7561 aagggtgact tagtggcgcg atttggtgcc gatccgacgt taaaacgtca ggatattcgc
     7621 aatatggtcg cgactttgaa aaaatctggc gtcaaccaaa tcgatggcaa tgtgttgata
     7681 gatacctcca ttttcgccag ccacgataaa gcccccggct ggccatggaa tgacatgaca
     7741 caatgcttta gcgctccgcc tgccgccgcc atagttgacc gcaactgttt ctccgtctcg
     7801 ctctacagtg cccaaaaacc cggtgatatg gcctttatac gcgtggcatc ttattacccc
     7861 gttacgatgt tcagccaggt acgcaccctc ccccgtggtt ctgccgaagc gcaatactgc
     7921 gaactggatg tcgtaccagg cgacctgaac cgctttacgc tgacgggatg cctgccacaa
     7981 cgttctgagc cgctcccgtt ggcttttgcc gtgcaggatg gagccagtta tgccggtgca
     8041 attctgaaag atgagttaaa acaggcgggt atcacctgga gcggaacact gctgcgccag
     8101 actcaggtta acgaacctgg aacggtagtt gccagtaaac agtcggcccc gctgcacgat
     8161 ctgcttaaga ttatgctgaa aaagtcggat aacatgatcg ccgatacggt tttccgcatg
     8221 ataggccatg cgcgcttcaa tgtacctggt acatggcggg ccgggtcgga cgctgtgcgt
     8281 cagatcctgc gccagcaagc cggtgtcgat attggaaaca ccattattgc cgatggttca
     8341 gggctttcgc ggcataacct gattgccccc gccaccatga tgcaggtgct gcaatacatt
     8401 gcccaacacg acaatgaact taactttatc tccatgctgc cgctggcggg ctatgacggc
     8461 tctttgcagt accgtgcagg tctgcatcag gcgggcgtgg atggaaaagt ctcagcgaaa
     8521 accggttcgt tgcagggggt atataacctg gcgggattca ttaccacagc gagcgggcaa
     8581 cgaatggcgt ttgtgcaata tctttctggc tatgcagtag aacctgcgga tcagcgtaat
     8641 cgccgtattc cgttagtgcg ttttgaaagc cgtttgtata aagatattta tcagaacaat
     8701 tagtcaaaaa gaaaccccgg cacatggccg gggcttcaga ttattgacaa agtgcgcttt
     8761 gtttatgccg gatgcggcgt aaacgcctta tccggcctac aaaatcgtgc aaattcaaca
     8821 tattgcaatt ctcttgtagg cctgataagc gtagcgcatc aggctgattt ggcgtttatc
     8881 atcagtgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg
     8941 tcatcccagt cttcatcatc ctcttcagca atctcttcaa gctgctggcg atgataatcg
     9001 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
     9061 tcaaggataa aggtcatcac atcccagcag agatctttca cgcccagtcc gctcgccgca
     9121 gagatcagat aatatttatc ttcccagccc agcgcttcag caatcgcttt cgctttctct
     9181 tcagcttctg ccttatccag cagatcgatc ttattgaaca ctaaccaacg cggtttcgcc
     9241 gccagatcct ggctgtattt ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga
     9301 tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg
     9361 tgcttcagga agcggatacc cagacctgcg ccttccgcag cgccttcaat cagtcctgga
     9421 atatcggcaa caacgaagct cttttcgttg tccattcgta ccacacccag acttggcacc
     9481 agagtggtaa acggataatc cgccactttc ggtttagccg ccgataccgc gcgaataaag
     9541 gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc
     9601 agcagcagct cacgcttatc acccggcgta ccgttggttt tctgccgtgg tgtacggtta
     9661 acggacgatt tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
     9721 cgctgaccgt gtttagtcat atcgcccatg gtttcaccag taccctggtc gattacacgc
     9781 gtacctaccg gcactttaat cgtcacgtct ttaccgcgct taccggtaca atcgcggctt
     9841 gcgccgttct gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg
     9901 ttcaggtttt cgtcggcttc catccatacg tcaccaccat caccaccgtc gccgccatcc
     9961 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
    10021 cctgcaacga ccagaatcga tgcttcatca acaaacttca ttttattctc cataaatcat
    10081 tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga
    10141 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
    10201 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
    10261 aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga
    10321 gccatcgctt ccgccagggc gccatatcct accaatgtat tcagtccgca aaaaattaaa
    10381 catgcgagct gccagtggct aagctgcgct atcactccag gctttgccag agggaagagc
    10441 gcaattgtac ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat
    10501 aaaacctttt gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac cccaaagata
    10561 actccccagg tgtaatcggt gagctttgta aatatctcga ccagactggt gttaaaaaac
    10621 atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtgctgcg cattttctct
    10681 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aatcacctgc
    10741 gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc
    10801 ccggcggtcg ccactgccaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
    10861 ggcaacctct tcttcaccgc aagaatggca ccaaggccaa tactcgccat caagaaacgg
    10921 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac
    10981 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
    11041 ataccgtatt ccctgcaaag cgcatttgcg gattaacgtc gcaccatgcg catttttacc
    11101 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
    11161 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
    11221 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
    11281 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
    11341 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
    11401 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
    11461 cagtaatttt cacatcagtg aaccactgac gatgaccctg ctgcttacga tagtgtttac
    11521 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
    11581 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
    11641 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
    11701 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
    11761 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
    11821 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
    11881 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc
    11941 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
    12001 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
    12061 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt
    12121 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
    12181 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
    12241 tatccacacg gcgactctgc tacacgacga cgttgtggat gaatcagata tgcgcagggg
    12301 taaagctacc gccaacgccg catttggcaa tgccgccagc gtgctggtag gcgattttat
    12361 ttatacccgc gcgttccaga tgatgaccag cctcggttca ctcaaagtgc tggaagtcat
    12421 gtcagaagct gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga
    12481 tccggacatc actgaagaaa actacatgcg cgttatctat agcaaaaccg cgcgtctgtt
    12541 tgaggctgcc gcgcagtgtt ccgggattct ggctggctgt acgccggagg aggagaaagg
    12601 cctgcaggat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
    12661 ttacaatgcc gatggcgaac agttaggtaa aaatgtcggc gacgatctga acgaaggtaa
    12721 accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg cacagatgat
    12781 ccgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat
    12841 gaacgcttgt ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc
    12901 catcgcagca ttacaggtgc tcccggacac cccttggcga gaagcactca tcggcctcgc
    12961 gcacatcgct gttcaacgcg atcgttaatc ccctcccttc atcccgcgca gtgcgcggga
    13021 tgattccagt aaattccata aaaacactaa ttcagcgcta taacacctga aaaaaaaggg
    13081 cgatgaatct ttatatattc tgaatattca cactctttac aggaactttt tagagcaata
    13141 ggccatcagg agtatagtga tgcccgacag aagaagtgtt ctgaatgaaa gcgactaact
    13201 taaggagtga ggaaagatgg aaagtaattt cattgactgg catcccgctg acatcattgc
    13261 gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct cgcagaaatg gtttgagttc
    13321 ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa ggagagatga ttattgcgaa
    13381 agccctggga actgacccct gggttatctg gccatcacgc taccatgatc cgcagaccca
    13441 tgagtttatc gacagaacgc agttgatgcg tagctacact aaaccgaaaa aatgagtggt
    13501 ctggcggtag ccccgcgaac ggggctgcca gctctcagac gattactcgc ctttcacacg
    13561 ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc cacgatcgat
    13621 gtgataaata cgatcaacca ccgtcgtccc ttccgcaata cagccagcca gcaccaggct
    13681 tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca gaaagttttt caacaccgtg
    13741 acaaataacg gtattgcttt cgatttcggc atgcgcgccc atacggctaa gctccggcac
    13801 atgcataaag cggttttcaa agaccgtttc ggtgatgaat ccggtccctt ctgccaccag
    13861 gttcaacagc gtgaactggg cctgcatatc ggtcgggaat gccggatgcg gcgcggtacg
    13921 tacgttaaca gccttcggac gtttgccatg catatccagg ctaatccagt cttcgccgac
    13981 ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg gcgtcgagag tatctggctg
    14041 cgcgttacgg cagataattt tgccgcgaga aatcgccgcc gccaccagga aagtaccggt
    14101 ttcgatacga tccggtagca cgcgatagac accgccgcct aaacgttcca caccttcgat
    14161 gacgatacga tcggtgccct gaccgctaat tttcgcaccc agcgtaatca ggaagttcgc
    14221 ggtatcgacg atttccggtt cacgcgctgc attttcaata atcgtggtac cttccgccag
    14281 ggttgcagca cacatgatgg tcaccgttgc gccaacgctg actttatcca tcacgatatg
    14341 tgcgcctttc agacgaccat cgacggaagc tttaacgtaa ccttcttcca gtttgatggt
    14401 cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac cgatcgtaca
    14461 accgccaggc agtgaaactt gcccctgacc aaagcgcgct accagcggcc ccagtgccca
    14521 gatagaagca cgcatggttt taaccagatc gtaaggtgcg cagaatacat taacgtcgcg
    14581 ggcatcaata tgcacagaac cattacgttc tactttcgca cccagctggc ttagcagctt
    14641 cattgatgta tcgacgtctt tcagttttgg gacgttctgg atctctaccg gttcttccgc
    14701 cagtagagcg gcaaaaagga taggcagagc agcattttta gcgccggaaa ttgtgacttc
    14761 gccctggagc ttcgttggcc cctgaacacg aaatttatcc atttagtttg ttctcagtta
    14821 acaattcata tccgctaccg gcgaatcgcc catagctcaa aagccgttca gtttgcgatc
    14881 gcgcgcccac tccgcagggg tatacgcttt gatcgacaca gcatgaatgc ggttatccgc
    14941 aatatattcc atcagcgggc catagaccgt ctgctgtttt ttaacccgac tcatgccgtc
    15001 aaacaactca cccacggcaa taacctgaaa gtggctgcca tcgccggaaa cgtggacttc
    15061 ctggagggag agagcgttca tcaacacgct ctgaatttca ttattttcca tgggatcttc
    15121 aatcatcagt taataaacca gcgaaacatc ttagagcaaa gttgcgctgg cataaataag
    15181 caaaaagcct cgctgataaa tcagacaagg ctcgacttgc aggcaggttt gccggacagg
    15241 cggttaacgc catatccggc ctgaaaaaat ttaacgaggc agaacatcag caggcaaatt
    15301 atacaatttc gcgagggtat acactttgtc gtttaccccc tgaagcgtca cgttgttgcc
    15361 ctgctttttc gccagatcga taagatggag cagcagtgcc agtccacccg tatccacgcg
    15421 ggacacacgg ctaagatcga tgcaggtaat ccccttcacc gcttcctcac gcatttccca
    15481 aagcggtagc agaacgtcct gatccagctc tccggataac gccagcgtgt cacccgtctg
    15541 catccagctc agtgactcgc tcattatttt ttctcttcca gagtgatttt ctgttgagaa
    15601 atcgatttca gttgcgcagt caggccgtcg atacctttgg tacgcagcag cgttccccac
    15661 tcgttttgtt tggtggtgat catactgacg ccttcagcaa tcatgtcgta agcctgccag
    15721 tttcccgtct gggagttttt acgccactgg aagtccagac gcaccggcgg acggccattc
    15781 gggtcaataa tggtaacgcg aataggcaca atggttttat cgcccagcgg ctgttctggc
    15841 gcaatctgat aggtttgacc gtgatacatc gccagcgcct gaccgtaagc ctgcttcagg
    15901 tactcacgga aagcggcaaa gtaggcttca cgttgagcag gggtcgcact cttgtaatac
    15961 tggcccagca ccagcgcacc ggcgtatttc acctgtacgt atggcagcag ttcctgatca
    16021 acaatggtac gcagataatc cgggttggcc cgaatttgcg gttgctcatt cttcaggcga
    16081 tcgaacgttt tctgcgccgc ctcgtccatc agcttataag gattggtctg gtctgccgcg
    16141 gttgccgcac tcagaggtgc aatcaccagc aaagcgacca tcattaaacg tttaaacatg
    16201 cgtcggttct cctgaaatta tttcgttgta cccacaggtt cagtggtttc attattacct
    16261 ggcgcagcag ctggcgcatc gccactattc ttattgtcat cgcctttact accgtaaagg
    16321 aactgaccaa tgagatcttc cagcaccatc gcagacttag tgtcctgaat tgtatcgcca
    16381 tccttcagga tagcagtccc cagttccggg tcttcaaaac cgacgtttaa tgccagatat
    16441 tgttccccca gcaggccgga agtacgaatg ctcagcgaac tggtatctgg aatgtggtta
    16501 taacgttgtt caatttccag cgttacgcgc ggcagatagg ttttcgggtc cagcgtaata
    16561 tccgccaccc gacccacaac aacgccacca atactgaccg gagagcgcgc tttcaggccg
    16621 ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat ggacgtcacg
    16681 ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg ctgctaataa aaagataccc
    16741 acccaaattt catttttttt cgtttgcatg aactcaattc ccaaacatca atgcggtcag
    16801 cacaaaatcc agccccagaa cagccagaga cgagtggaca acggtgcgag tggttgcccg
    16861 gctaatcccg gcagacgtcg ggatggtgtc gtagccgtta aacaacgaaa tccacgtcac
    16921 cgtgatggcg aacaccacgc tcttaatcag acagttggcc agatccatac gccagtcgac
    16981 ggcattttgc attgccgacc agaagaaccc gctatcaatg cctttccaac tgacgccgac
    17041 cagagatccg ccccagatcc ccacggcgac gaaaataacc gtcaacagtg gtaatgaaat
    17101 aaccccagcc cagaaacggg gagaaataac ccgacgcagc ggatccaccg ccatcatctc
    17161 catactggag agttgctctg tagcgcgcat caggccgatt tctgcggtta gcgccgaacc
    17221 agcacgcccg gcaaacaaca acgcggcaac caccggcccc agttcacgca gtagcgataa
    17281 cgccaccagc atacccagac tggtttccgc actataagtg gtcagaacca gataaccttg
    17341 cagccccaac accattccga tgaacacgcc agaaaccaca ataatcagca tcgacaggac
    17401 gccgacatta tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt ccggtttgcc
    17461 gaccagcgca ttgaataaca ttaacccggc ccgcccgaac gttctcaggg ttttaatccc
    17521 tttatgtccg agcgacgcca gcgcatttaa cagcatgagt ggcttaactc cctggtaaaa
    17581 gatcagcgtg ataatcgccg gcaggatagc ggaacggaac aggcccgtca gctatcccgt
    17641 ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa cgcctgggca ctgccatgag
    17701 cgacaatttt tttgtccgcc aggatccagg cgtgatccgc aatacttaac acttccggca
    17761 catcgtgaga aaccaccaca caagtcacgc ccagcgcgct gttcagctca gaaatcagct
    17821 tcaccagtac gcccatggtg atgggatctt gcccaacaaa aggttcatca aacatgatga
    17881 gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc ccaccggaaa
    17941 gttcagaagg cattagttta gccgctccac gcagccccac ggcctccagc ttcatcatca
    18001 ccgtactatg caacaatggc gcgggtagtt gggtatgttc gcgcagtgga taggcgacgt
    18061 tgtcaaatac gttcatatca gtgaacaacg ccccggactg aaataacatg ctcatccgtt
    18121 tgcgcactgt atacaggcgc gaacgagaca tcgccggaat attctcacca tcgaaaagga
    18181 tctcaccatg atctggtgcg atttgcccgc caatcagacg gagtagcgtc gttttaccga
    18241 tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg cacggtcagg gaaatattat
    18301 cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg catatcgact aaattcgcca
    18361 cagactgctc cataattcac ccttcgtctt gcgttgattt tctaagcatg gcgctcaatt
    18421 taaccttgaa cccaacatat ttacagaata ttacccgccg tggttagcga aagctggcat
    18481 ttgttttact ttttagccgc ataaagtcaa aattaagaat ccgttacggc tttctgaaaa
    18541 tcttcagcgg accgacgagt atacctgaag aaaggacgtt agatgctttt agctacggca
    18601 ctgttaattg ttggtttact tttggtcgtt tacagtgccg accgcctggt ttttgccgcg
    18661 tctattcttt gccgaacctt tggcatcccg ccgctgatca tcggcatgac ggtggtcagt
    18721 attggtacat cgttaccaga aatcatcgtc tcgcttgccg cgtctctgca cgaacaacgc
    18781 gatttagccg ttggtacagc cctcggctca aacattatca atattttgct gatcctcggt
    18841 ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg ttctgcgtcg tgaattaccc
    18901 ttaatgttgt tggtcagcgt agtggccggt tccgtactct atgacggaca acttagtcgc
    18961 agcgatggta tctttctcct ctttctggct gtgctatggc tgctgttcat tgttaaactt
    19021 gcacgtcagg ctgaacgtca ggggactgac agcctgacca gagagcagct tgcagagctg
    19081 ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg
    19141 ccagtggcca cgcggatggt ggttgataac gccacggtgc tggcgaatta ctttgccatc
    19201 agcgagttga cgatgggtct gacggcaatt gctatcggaa ccagcctgcc ggaactggca
    19261 accgcaatag cgggggttcg caaaggtgaa aacgacattg ctgtcggaaa tatcattggc
    19321 gcaaacattt ttaatattgt catcgtgttg ggtttacccg cgctgataac gccaggagag
    19381 attgagccat tggcgtacag tcgtgactac agcgtgatgt tgctggtgag cattattttt
    19441 gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt attattaact
    19501 ggcggattta tcgtatggct ggcgatgttg tactggttat cgccaatact cgttgaataa
    19561 ctggaaacgc attatgtcgc acgtagagtt acaaccgggt tttgactttc agcaagcagg
    19621 taaagaagtc ctggcgattg aacgtgaatg cctggcggag cttgatcaat acatcaatca
    19681 gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg
    19741 gatggggaaa tcggggcaca tcgggcgcaa aatggccgca acgtttgcca gcaccggtac
    19801 accttcattt ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca tggtcacccc
    19861 acaggatgtg gtgattgcta tctctaactc aggtgaatcc agcgaaatca cggccttaat
    19921 tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc accggtcgcc cggagagcag
    19981 catggcgcgc gccgcagatg tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt
    20041 agggctggca ccgaccagca gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt
    20101 cgcgctgtta aaagcacgcg gctttactgc tgaagatttt gcgctctcac acccaggcgg
    20161 cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat
    20221 cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg ctggaagtta cccgcaaaaa
    20281 tcttggtatg actgtcattt gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg
    20341 tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt cagttaagta ttgccgatgt
    20401 gatgacgccg gggggaatac gtgtgcgccc tggcattctg gccgttgagg cactgaactt
    20461 aatgcagtcc cgccatatca cctccgtgat ggttgccgat ggcgaccatt tactcggagt
    20521 gttacatatg catgatttac tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca
    20581 atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc cgacgttata
    20641 gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg atggcgtact gtcagatggc
    20701 ctgatttata tgggcaataa tggcgaagag ctgaaagcgt tcaatgttcg tgacggttat
    20761 ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct
    20821 aaactggtag aagatcgttg tgccacattg gggatcactc acttgtatca ggggcagtca
    20881 aacaaactga tcgcctttag cgatctgctg gaaaaactgg cgattgcccc ggaaaatgtg
    20941 gcttatgtcg gcgatgatct catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc
    21001 gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg attacgtgac gcgcattgct
    21061 ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac tcctggcgca gggcaaactg
    21121 gatgaagcca aagggcaatc gatatgagta aagccagacg ttgggttatc attgtgctat
    21181 cactggcggt tctggtgatg atcggcattg atatggccga aaaagacgat accgcccagg
    21241 tggtcgtcaa caacaatgat cccacctata aaagcgagca tacggacacg ctcgtctata
    21301 acccagaagg ggcactaagc tatcgattga ttgctcaaca cgttgaatat tattccgatc
    21361 aggccgtttc gtggtttacg cagccggtac ttaccacgtt tgataaggat aaaatcccga
    21421 catggtccgt aaaagcagat aaagccaagc tgaccaatga ccggatgctc tatttatatg
    21481 gacacgttga agtcaacgca cttgtgccaa actctcaact tcgcagaatc acgacggata
    21541 acgcgcagat caatctggtg acgcaggatg ttacctctga agacctcgtc acgttatacg
    21601 gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg
    21661 agctgattga aaaggttaga acatcctatg aaattcaaaa caaacaaact cagccttaat
    21721 cttgtgcttg ccagctcact tctggccgcc agcattccgg catttgccgt aaccggagac
    21781 actgatcagc cgatccacat tgaatcggac cagcaatctc ttgatatgca aggcaacgtg
    21841 gttaccttta ccggtaatgt catcgtcacc cagggcacca tcaaaattaa tgccgacaaa
    21901 gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag tgattgacgg ctacggtaaa
    21961 ccggcaacgt tctaccagat gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag
    22021 atgcactacg aactggcaaa agattttgtc gttctgacgg gtaatgctta tctgcagcag
    22081 gtcgatagca acattaaggg cgataagatc acttacctgg taaaagagca gaaaatgcag
    22141 gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac
    22201 aaaaacaaca aaggccagac cccggcacag aagaagggta attaattcgt tatggcaaca
    22261 ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc
    22321 ctgaccgtca actccgggga aattgtcggt ctgctggggc caaacggtgc cggtaagacc
    22381 accactttct acatggttgt aggcattgtg ccgcgcgatg cgggcaacat cattattgat
    22441 gatgacgata tcagtctgct gcctctgcat gcacgcgcgc gccgcggtat cggctatctg
    22501 ccacaggaag cctccatttt ccgtcgcctc agcgtttacg ataacctgat ggcggtactg
    22561 caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa
    22621 gagtttcaca ttgagcacct gcgtgacagc atggggcagt cactctccgg gggtgaacgt
    22681 cgccgtgtag aaattgcccg cgcactggct gcgaatccga aatttattct gctcgacgaa
    22741 ccgtttgccg gggttgaccc gatctcggtt atcgacatta aacgcatcat tgagcacctg
    22801 cgcgacagcg gcctgggcgt gctgatcact gaccacaacg tgcgtgaaac actggcggtt
    22861 tgtgaacgcg cttatatcgt cagtcagggg catttgatcg cccacggcac gcctacagaa
    22921 atcttacaag acgaacacgt taagcgtgta taccttgggg aagacttcag actctgatag
    22981 ggtagaagtt tgcgacgttt tagcaggaga gtacgattct gaacatgaag caaggtttgc
    23041 aactcaggct tagccaacaa ctggcgatga cgccacagct ccaacaggca attcgtctgt
    23101 tgcagttgtc gacgctggaa cttcagcagg agctacagca ggcgctggag agtaatccgc
    23161 tgcttgagca aatcgacact catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa
    23221 cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg
    23281 ccagttggga caccatttac accgctggta caccatccgg caccagcggt gactacattg
    23341 acgatgagct gccggtctat cagggcgaaa cgacgcagac cttgcaggat tacctgatgt
    23401 ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg
    23461 atgccgttga tgacatcggt tatctgactg tcccgctgga agatattctc gaaagtatgg
    23521 gcgatgaaga gatcgacatc gacgaggttg aagccgtcct taagcggatc caacggtttg
    23581 atccggtcgg tgtagcggca aaagatctgc gtgactgcct gctaatccaa ctctcccaat
    23641 tcgataaaac cacgccgtgg ctggaagagg ccagactgat cattagcgat catctcgatc
    23701 tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc
    23761 tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg ccccgggcag tcgatccaga
    23821 ctggcgaacc tgagtatgtc attccagatg tgctggtgcg taagcataac ggtcactgga
    23881 cggtagaact caacagtgac agcattccgc gtctgcaaat caaccagcac tacgcctcga
    23941 tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat ccgcagcaat ctgcaggatg
    24001 ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct
    24061 gtatcgttga acagcagcaa gccttctttg agcaaggtga agaatatatg aaaccgatgg
    24121 tactggccga tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca
    24181 cgcaaaaata cctgcatagt ccacgaggca tttttgaact gaagtatttc ttttccagtc
    24241 acatcaatac cgagggcggc ggcgaagctt cctccacggc gattcgtgcg ctggtgaaga
    24301 aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta acctctttgc
    24361 tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc gaagtaccga gagtctttat
    24421 ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa ggaagacact
    24481 atgcagctca acattaccgg aaataacgtc gagatcaccg aggcactgcg cgaatttgtt
    24541 acagccaaat ttgccaaact tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt
    24601 ctgaaagtgg agaaagtcac ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa
    24661 attcatgcca gcgcggaagg tcaggacatg tacgctgcca ttgatggctt aattgataag
    24721 ctggcacgcc agttgaccaa acataaagat aaactgaaac aacactaatt gtccgggcaa
    24781 ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc catttgtacg gttaatgctc
    24841 cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg aaattatgac aaataatgat
    24901 acaactctac agcttagcag tgttcttaac agggaatgta cgcgaagccg cgtccactgt
    24961 cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca acttagcctg
    25021 ccgcctcagg tggtttttga agctatcctg acgcgggaaa aaatgggcag taccggtatc
    25081 ggcaatggta ttgccattcc gcatggcaaa ctggaagaag atactctgcg cgccgttggc
    25141 gttttcgttc agctcgaaac gcctatagct ttcgatgcca tcgacaacca accggtggac
    25201 ttactttttg ccctgctggt gtcggcagac caaactaaaa cgcacctgca tactctgtcg
    25261 ctggtggcga aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc agcccagagc
    25321 gatgaagagc tgtatcaaat cattacggat accgaaggta ctccggatga agcgtagtta
    25381 ttcggtaatg tctcttttag acgttgtgag gagaaacagt acatggtact gatgatcgtc
    25441 agcggacgtt caggttcagg taaatctgtc gccctgcgtg cgctggaaga tatgggtttt
    25501 tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc tggctcgaac cctggccgat
    25561 cgcgagattt ctgccgccgt cagcattgat gttcgtaata tgccggagtc accagaaata
    25621 ttcgaacagg cgatgagtaa cctgcctgac gctttctcac cgcaactcct gttcctggat
    25681 gccgaccgta ataccttaat tcgtcgttac agtgacacgc gccgactgca tccgctttcc
    25741 agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa gcgatttgct ggagcctctg
    25801 cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa
    25861 atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct
    25921 ttcggcttca aacacggtat ccctatcgat gcagattacg tctttgacgt gcgcttcttg
    25981 ccgaacccgc actgggatcc gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc
    26041 gcgttcctcg accgccacac agaagtacac aattttatct accagacgcg aagctatctt
    26101 gagctatggt tacctatgct ggaaaccaac aaccgtagct acttgacggt cgccattggt
    26161 tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc aactggcaga ctacttccgc
    26221 tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg
    26281 tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca tgcccggcct gcaatgaagc
    26341 tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg
    26401 aggccgaagc caacagcgtt attgcgctgc tgatgttgga ttctgccaaa ggacggcaga
    26461 ttgaagttga agcgaccggc ccacaggaag aggaagcact ggccgccgtt atcgccctct
    26521 ttaactctgg ttttgatgaa gattaatctt catcactttg acatacaact cccttcaaac
    26581 tccccccgac aataagaaaa tcacgtactg aaatcgttct caatcaacgt catttgtaca
    26641 ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaggc attgtttagg
    26701 ttttgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca ggagatggca
    26761 tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa tgttatgcgc
    26821 atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat ggtggcgata
    26881 aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta aaagactgga
    26941 gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt tatgtctacg
    27001 atgtgattgc agattttgac ggcaaactct ccattacgct gaccgaattt gctgaacaat
    27061 gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg aagcgttatt
    27121 tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc tgtcaccgca
    27181 tcagtgagtc agaggttatc ccggtcgtct gcgacaacat tggtgaaagc acattgattc
    27241 cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta tggaaacgat
    27301 ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct cgtgagagca
    27361 aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgttgttgc ataaacggct
    27421 caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc acgtagcccg
    27481 atggcgagga gactttgaaa cgaagtggat taggtaatac agctgccagt aatgcagctt
    27541 ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca gcttcgacgc
    27601 caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata cgctttttgc
    27661 tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt acccagctac
    27721 gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa gcaccgcgaa
    27781 tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg acatcaaagc
    27841 cccagtgctc agaaaatttc tgatcttctg cggcaatcac cgccagtccc atccacggcg
    27901 agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg ccatgcagcc
    27961 aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca acactaaaca
    28021 acgcgatgcc cccgccccag aaaacggcga ggacaaccat taacagcaaa agaaaacggc
    28081 gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca gcacgcggga
    28141 aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca tatatgctgg
    28201 ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac agggcacatg
    28261 ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga tggtcaaacg
    28321 cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa aaccaagcgg
    28381 ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa cggtgcattc
    28441 gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc ccggcacaat
    28501 caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac cgcgcgttat
    28561 tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc gcttcgccag ttaaatggtt
    28621 gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac cgctgcgtga
    28681 aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga cgccgcatcc
    28741 gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga ctgaaacaca
    28801 ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg taacaaatca
    28861 tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc ccttgtaact
    28921 catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt attcgcgcac
    28981 cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc agcacttcta
    29041 cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta tcttcccagg
    29101 caggaaggtc aggagactga atttgctgac ccagctgttg cagatggcgt aaccccactg
    29161 accccgccgc acctttaatt ttatgtcctt cctcaacaat gcctttttta tcctgcgccg
    29221 tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac accgctaacc
    29281 cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg ggaatatcga
    29341 gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca tcatcctggg
    29401 tatcccagaa tttcttgatc atcgcggtta gcgccggaac agaaagcggc ttactcagca
    29461 catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta gcggttaagg
    29521 ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt cagttcacga gagatatcca
    29581 gcccggtcat atctggcaac tgaatatcca gtaacaccag gtcgtattcg cccggtttaa
    29641 acatctctag cgccgccttg ccggtcatgg cgacatcaac gctgttacct aatttttcca
    29701 gcacagaacg cgcaacaatc acgttcagtt caatgtcttc caccagcagc acattcagcg
    29761 ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct accgacggtg
    29821 cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc gtaatatcgc
    29881 cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg ccggtggcag
    29941 gtttaccgcc atgactgtct ttcacctggt aatacatggc gaaaatttta tccagctcat
    30001 cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg ccttcatcgt
    30061 agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta ctgatgaggt
    30121 tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt aatggcagcg
    30181 tcggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggag agattttcca
    30241 gatcggcaag gaagctggtg aaatcaaccg gttgattatc aagctggacc ttgcgccgtt
    30301 ccatcttatc catgtcgata atatcgttaa agatattccc cagcgtgacg gccgaaacat
    30361 ggatggtctt gagatatttt tcctgctcgg cggtgagttc ggtatccagc agaatgcggc
    30421 tcagaccgac gataccgttc agcggtgtac gcaattcgtg actgatggtg gagataaacg
    30481 tcgttttgtc gcggctggcc cgttcaagcg catcctgata ccgcttacgc tcggtaatgt
    30541 ctcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac ggcactttac
    30601 ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag ccactgttca taggtcagtg
    30661 acacattatg acggaacact ttttcatcgg tttcaatgac ttttgcggcg gcttccggtg
    30721 agtaaacatc aacaggtttc aggtgaacca gttgtttttc gctttttccg gtcagcagct
    30781 ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa accaggtcgg
    30841 gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga atttgtgtct
    30901 cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt tctgcttccg
    30961 ctttttcacg aacggcgatt tcctgattta gctgggcaat attatctttt aactgaacgt
    31021 tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt gacagacgtt
    31081 gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc cacggcgtaa
    31141 tcaacaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga ccatgcagca
    31201 ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc gccagcaaca
    31261 ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc gccagcagac
    31321 gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac ccaattctga
    31381 cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct actccgttgg
    31441 aggtagccac ggacgtccca gcgccgatcc ctgtacgcca tgttcattca gatagcgatc
    31501 cagctcgacc attcccgtcc atcgattttc gcaccacaga ggagcaagca gcgtcgggcg
    31561 acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc gaatcatttc
    31621 tccggcagtg aacgtgtaat cctccagttc aataccgttc aaacgccccg cttcccaggc
    31681 tttcgccata atgctgcctt tcacaatatg caacggatgt agctttatac cgtcaacacc
    31741 ggtttccact acccgctcca gcgtttgcag acattcagcc agcccttcgc ctggcagacc
    31801 gacaattaaa tgcgagcaca ccttcagccc gcgctggcgt gccagctgcg tcgtgcgctg
    31861 ataacaggcg aaatcatgac cgcgattaat gcgatgtagc gttttgtcgt gcacggtttg
    31921 cagccccagc tccagccaca cttcgtagcc ctggtcttta tattcgcaaa gcagatccag
    31981 caccgcatcc ggcacgcagt ccgggcgcgt gccgacacac aaaccgacaa tattggcctg
    32041 gctcaccgcc tgttgataca tcgaacgcag cacctgaact tcggcaaagg tgctggtata
    32101 cgcctggaaa taggcgaggt aacgtttcgc gcgattaatt aaattcgcct gatgcgccag
    32161 ctgttcggca atggaacgat gctgctgcgc ttcgtcggca aacgaggcaa cattacagaa
    32221 ggtgcagccg ccgcgcccga tggtgccgtc gcgattcggg caactgaaac cgccgtgcag
    32281 cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa acatattgac
    32341 taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca aagcctgcca
    32401 tttttagctc aattcggcga tgacctggat caatcgtccc ggcccgcttt tatcaactgc
    32461 ataatcaatc aaaattaccg aaatttcatg cataatcaca taaatcactt ttgcttatct
    32521 tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca tgaaacgtca
    32581 ttaccaatta atgcagtata aaatgctggt tttctcgtca attcaaggca ggataagggt
    32641 taacacgcct ttatgacagt caggaattga ctgtttctct aacgacttcc cttttagcct
    32701 taaagataaa atctatttta atttcagcca tttaataaag aattttgcgc taaagcacat
    32761 ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag ttggaaatcc
    32821 gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat tgagatcccc
    32881 tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga ggcgcgcgta
    32941 tgacacgcaa cccccgtcgc cacgctcttt ctgtacccgt gcgcagcggt tcggtagtgg
    33001 ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc cttgagaggg
    33061 ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac aaggtagtgc
    33121 gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc gccgatggta
    33181 aaaccggtga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt cgcatcgttg
    33241 cgcaggagcg cggctggcgt ttagcgaaaa actacgccgt cgggatgcta ttcctgaata
    33301 aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagaactg caacgcgaaa
    33361 ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtactg ggtgaaatcg
    33421 ccctctcctc tctgccccgc attgagcaaa tttttgtgaa cgccccggca ggctggcgtc
    33481 cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag cgtctcgaag
    33541 ccgacaaaga cttctacgtc tgtagcctat cgaatctggt gaacatctat aaaggtctgt
    33601 gtatgccggc ggatctgccg cgcttttatc tggatcttgc ggacctgcgt ctggaatcgg
    33661 ccatttgcct gttccaccag cgcttctcca ctaacaccgt accgcgctgg ccgctggcgc
    33721 aaccgttccg ctatctggcg cataacggtg aaatcaacac catcactggt aaccgccaat
    33781 gggcgcgtgc gcgtacctat aaattccaga cgccgcttat ccctgatctg cacgacgccg
    33841 caccgttcgt caacgaaacc ggctctgact ccagttcgat ggataacatg ctggaactcc
    33901 tgctggcagg cgggatggat atcatccgcg ccatgcgtct gttagtgcca cccgcctggc
    33961 agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac tccatgcata
    34021 tggagccgtg ggatggcccg gcgggcatcg tgatgtccga cggtcgtttt gccgcctgta
    34081 acctcgaccg taacggtctg cgtccggcgc gctacgtcat caccaaagat aagctcatca
    34141 cctgcgcctc tgaagtcggt atctgggatt accagcctga cgaagtggtc gaaaaaggcc
    34201 gcgtcgggcc aggcgaactg atggttatcg acacccgcag tgggcatatt ctgcactcgg
    34261 cagaaaccga tgacgatctg aaaagccgcc atccatataa agagtggatg gagaaaaacg
    34321 tccgccgact ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc cgcgaactgg
    34381 acgacgacac gcttgccagc taccagaaac agtttaacta cagcgcggaa gagctggact
    34441 ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt cggttcgatg ggcgatgata
    34501 ccccattcgc cgtgctctcc agtcagccgc gcattattta cgactacttc cgccagcagt
    34561 ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt atgtcgctcg
    34621 ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc agagggccag gcgcaccgtt
    34681 taagctttaa atcgccgatt ctgctctact ccgatttcaa acagctcacg acgatgaaag
    34741 aggagcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa accacgctcg
    34801 aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc ggcaccgtgc
    34861 tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttcca gccccgatgg
    34921 cggttggcgc gatccagacc cgtctggtcg atcaaagcct gcgttgcgat gccaacatca
    34981 tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg ggcttcggcg
    35041 cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta gacacccatg
    35101 cgattgccaa agattatcgt accgtgatgc tcaactaccg taacggcatc aacaaaggct
    35161 tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc tgctcgaaac
    35221 tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct gtgcttccag ggggcggtca
    35281 gccgcattgg tggggcaagc tttgaagact tccagcagga tctgctgaat ctgtcgaaac
    35341 gtgcctggct ggcgcgtaag cccatcagcc agggcggcct gctgaaatac gtccacggcg
    35401 gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct gcaacaagcg gtacaaagcg
    35461 gcgagtacag cgactatcag gaatacgcga agctggttaa tgagcgtccg gcaaccacgc
    35521 tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct gatgttgaac
    35581 cggcaagcga actgtttaaa cgcttcgata ccgctgcgat gtctatcggc gcgttaagcc
    35641 cggaagccca cgaggcgctg gcggaagcga tgaacagcat cggcggtaac tcaaactccg
    35701 gtgaaggcgg tgaagatccg gcgcgctacg gcaccaacaa agtgtcgcgc atcaagcagg
    35761 tggcttccgg tcgctttggg gttactccgg cgtatctggt caatgccgac gtcattcaga
    35821 ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca gttgccgggt gataaagtca
    35881 ctccttacat cgccaaactg cgctattcgg tgcccggagt gacgctgatc tccccgccgc
    35941 cgcaccacga tatctactct atcgaggact tagcgcagct cattttcgac ctcaagcagg
    36001 ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta ggcaccatcg
    36061 cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat gacggtggca
    36121 ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg cccgtgggag ctggggcttg
    36181 ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca taagatccgt ttgcaggtcg
    36241 atggcggcct gaaaaccggt gttgatatca tcaaagcggc gattctcggc gcagaaagct
    36301 tcggcttcgg cactggccca atggtcgcgc tcggctgtaa atatctgcgt atttgccacc
    36361 tgaacaactg cgcaacgggt gtggcaactc aggatgacaa actgcgtaag aatcactatc
    36421 acggcctgcc gttcaaggtg acgaattact ttgagtttat cgcccgtgaa acccgcgagc
    36481 tgatggcaca gctgggggta acgcggctgg tggatctgat tggtcgcacc gacctgctga
    36541 aagagctgga cggtttcacc gccaaacagc agaaactggc gctgtcgaag ctgctggaga
    36601 ccgccgaacc gcatccaggt aaggcactct actgcaccga aaacaacccg ccgtttgata
    36661 acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat gaacgccaga
    36721 gcaaaacctt ctggttcgat atccgcaata ccgatcgctc cgtgggcgcg tcgctttcag
    36781 gctatatcgc ccagacgcac ggcgatcagg ggctggcagc cgatcctatc aaagcgtact
    36841 tcaacggcac cgcaggccag agtttcggtg tatggaacgc gggcggcgtg gaactgtacc
    36901 tgaccgggga tgccaacgac tatgtcggta aaggcatggc gggcggctta atcgccattc
    36961 gtcctccggt tggttccgcc ttccgcagcc atgaagcaag tattatcggc aacacctgcc
    37021 tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg cgcgggtgaa cgtttcggcg
    37081 tgcggaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc tgtgaatata
    37141 tgacgggcgg tattgtctgt attctgggta aaaccggcgt taacttcggt gcgggcatga
    37201 ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc gttaacccgg
    37261 aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat ctgcgcggtc
    37321 ttatcaccga gcatgttcag catactggct ctcagcgcgg tgaagagatt ctggcgaact
    37381 ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat gtaaaagcac
    37441 tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag taaggggtag
    37501 caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat ccgccaaaga
    37561 aaccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt tccgaaggcc
    37621 aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc gagtggaaat
    37681 gtccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg cgtatttttg
    37741 aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtttgcgga cgagtctgcc
    37801 cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga tgagtttggc gcggtgacca
    37861 tcggcaacat tgagcgttat atcaatgata aagcgttcga aatgggctgg cgtccggata
    37921 tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat cggcgcaggc ccggcaggtc
    37981 tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc gaccgtcatc
    38041 cagaaattgg cgggctgctg accttcggta ttccggcctt caagctggaa aaagaggtaa
    38101 tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga attcaaactc aataccgaag
    38161 tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtc ttccttggcg
    38221 tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat ggcgtgtacg
    38281 cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt gaaacccgcg
    38341 acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt ggcgacactg
    38401 cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa gcacgttacc tgtgcctatc
    38461 gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg cgggaagaag
    38521 gcgtagagtt caaattcaac gtccagccac tggggattga agtgaacggt aacggcaaag
    38581 tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa ggccgtcgcc
    38641 gcgcggagat cgttgcaggt tccgaacata tcgttccggc agatgcagtg atcatggcgt
    38701 ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag ctggattcac
    38761 aaggccgcat catcgccccg gaaggcagcg acaacgcctt ccagaccagc aacccgaaaa
    38821 tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtaaccgct attgccgaag
    38881 gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagctctg tcccagcgcc
    38941 gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaag cgcctcgcat
    39001 aatctgaaga aaaccaatgt aatacgctca tctggattgt taccggtaga tgcccggcgt
    39061 atagctgatt ccattattca acgcagcgcc atataaaggg acagataatg gaatcgctct
    39121 cagagagaac ctcaacaggc taccagcaaa tccacgacgg tattattcac ctggtcgata
    39181 gcgcccggac ggaaacggta cgtagcgtta acgcgctgat gaccgcgaca tactgggaaa
    39241 ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc aagggctgcg tatggtgcgc
    39301 agctaatcaa gcgactatca aaggatttaa gtctaaggta taagcgtggt ttctctgcaa
    39361 aaaacttacg ccaaatgagg cttttttacc tcttttttca acatgttgaa attcgccaga
    39421 cagtgtctgg cgaattaaca ccattgggaa ttccgcagac accatctgca gaattcccat
    39481 cggcaaagat ttggcagacg ctgtctgcca aattgagagg ctgtttgata tttccacagc
    39541 ttttgtattg catctcctat tttcggtttg tatattcatg caaataaacg aaggaaagtt
    39601 gccgtgattt acattgaaca gcttgaatta atccataaaa gtggtgatgt gttgtatcca
    39661 gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc accttgttcc attcggctta
    39721 aataagactc acgaccttct agaggtggaa gatgcttcag aagcaattcg gctggtgatt
    39781 gatgaacgtc attccattcg ctgcagtaca cttacagcaa ctattacgaa taaaaagggc
    39841 aagcgaatta agcggacagg tatatacagt atcaagggtg ttaacataaa agaatacaac
    39901 gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg gctctgtgcc aaagatgggt
    39961 caggtcacac ctgatctaat aaatttaagc cattaaatca gccttaacct tcaccaccac
    40021 ctttttaatc tcacccggct cgccaacaac gcatcccggc ttatgcggct ctcctagcat
    40081 aaacacggcg aacattcccg gtttcaggat gatggcttgc tcgttttcaa tggtgctgca
    40141 aagctggtaa tcatcctcat ggtggaactc ttcacactga cgcgcagtgc ctgccatgcc
    40201 aaacagaatc cgttcttcac cgtttaataa cagctggata tcaatgtatt gctcgtgcag
    40261 ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc atgacattca taaaaatatt
    40321 gtcgccctgt aattcgtaac gacccggcgc tttttcttgc ggtctggcag ctaatgccag
    40381 cgttaacgcg tcctgtaacg caggatgtaa cccagcagac ggtaatgacc gtacttcacc
    40441 catcatcata atttttctcc ctgggccaac agcgctgccc caagtaaacc tgcatcatgg
    40501 cggtagtgcg ccgccagtaa atcaacatga aataccgctg gctcctgcgc cagatacgtt
    40561 tccaccaacg ccagataccc ttctgccaga ccaacgctgc caccgaccac cacgcactgg
    40621 caatcagttg tggctttaat atcagcgatc agccttgcca gcacatgtgc ggagcggtga
    40681 atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg tgaaaatagt tctcgcatcc
    40741 gcgcccatga gttcaccttg cgctgccgct gcaataccgc gaccagaagc aatcgcttcc
    40801 acgcaacctg tgcgtccaca gccgcagact gggccgtgtg gatcggcaag cgtatgcccg
    40861 atatgccccg ccagaccgcc agggccggta agcagtttac cgccgctcac tacaccgccg
    40921 ccgacgccag tggaaacggt gataaacacc atctcggtta catcgccttc cagcgcctga
    40981 tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa tggtcggcaa attggtaagt
    41041 tgttccagcg ttttgactaa cggaaagtgt agcaatccac cgagattatg cggattcagc
    41101 gccagcaaac tgccgtcgcg aataattccg gttgaagcga tggcaacccg ctgcgcatga
    41161 gcttgcaacg gagagactaa tgcggataag gcatcacgca aggcttctgg tgtctggctg
    41221 gctggcgtag gaagttcacg acgatcgcgg atctgcccgt cagcgccaat cagcgcggcg
    41281 gcaagtttag taccgccgat atcaatcgcc agtgtggtca tagcaccgcc tttttcatcg
    41341 ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat ggcagaaccg accgtcaccg
    41401 cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg cgtgttgtaa cgcccttcgg
    41461 caatcacccg acatccggct tcgctcaacg ttttcaccag cgccagatcc ggctcttctg
    41521 gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat aatttcggct cccagctttt
    41581 ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat cgccagtaaa ccgtgatgat
    41641 gtatacgtgc cagcagcgtt tcaacaggca ccggacgcgg gcggtcggtg ccgtcaatgg
    41701 cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc ttcaatatag gccgtgatgc
    41761 gtaccggaga atcctccaga tcgcgtttca caattccaat aatcggcacg ctcaccaccg
    41821 cacgcgtggc ttgcagattt gccacacctt cgatgcgaat ggcaaccgcg cccgcctgct
    41881 ctgccgctaa tgccatggct gcgacgattt cgggtttatc gagcgggctg tcaggaatcg
    41941 gctggcagga gacaatcagg ccaccgttag cagcgatttt ttgatccagt tgtgcaagta
    42001 acgacataca tcttccctta gcgaaaggcc cggtacatag accgggcaac aggattaact
    42061 tttggttttg actaaatcgt ttttggcgct gccaaatggc acggcaccgc taaatggttt
    42121 gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc agccaacgct gaacgcggga
    42181 aggcatatcc agaccaatca gcaggatcac cacaaatgtc aggctgaacg agagcgatgc
    42241 cagcgcagta cccagatcca gacgttgagc gatcaacgcg ccgatgattg gggccagtgc
    42301 gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc gctgcacgct ggtcggtatc
    42361 gaaataaccg ccaatcagtt tcggtaagat cccggcgatc ccttgtccaa gcatttgctg
    42421 gaagaacagt aacagaccga gcacccagac gtttgcgccg ccaatcgcaa ataccggaat
    42481 aatcagcagc tgcgaggcca gcaggctaca aacgtacgct ttgcgggttc ccagccagtc
    42541 accgaggaag cctcctacgc agcatcccac ggccgcgcca aagccactaa agaacagcac
    42601 attggcgaca gtatgcgggt tataagccag atcggttttc agatacgttg gcagcagcgc
    42661 ctgaatcggc catgagtaga ggaaagcaaa caacacgacc accatcagca ttacgcccgt
    42721 tggccagcgt ttgcctgtac tctgcaccat aaagctgata aagattgcgg cgcataacag
    42781 cccaagaaca gcgacgatcg cggcattttg caggttaccg gcgaagcaga accacagcgc
    42841 agtagccgcc gccagtgtca ttacgatatt ggcaatgcga tgttcaccac ggtagagaat
    42901 atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc tctttccagt cttccgcttc
    42961 cgggatgttt ttacgcagcc agagagcaaa gatgattggc aaaatgccga taaagaacag
    43021 cgcacgccaa ccccagaccg gaaccaccag gctatagacc tgagcggcaa cgacggcccc
    43081 cacagagaag cctgaaatca aaaaaccact ggctttgtta cgcagatgtt ttggccagct
    43141 ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc atccccatgc cgatgaccag
    43201 acgagcgata aacatggtga tgtagcctgg cgcaaagccg caggccagcg tcccggccga
    43261 gaagagaacg atgctggtga ccattgccag acgacgcccg tagcggtcac ccatagcgcc
    43321 gagcatcagg ccgccgaacc agcgagagat aaaggctgcg gagatcagac ttgccgcctg
    43381 caccgtcgtc agcccgaatt caccctgtac ttcggtgagt accagggcga ttaaaacgaa
    43441 atcaaaaccg tcaagcagat atcccaacca ggcagcggaa aatgcgcgcc attgtgcacg
    43501 gttgagatgg cgataccacg ggatattctg ggttgtagta ctcattgtga gtctcccgcg
    43561 gtgggcgatg cccacacgct ttggtatgaa aattgtaggg tacagatgcg tttatttccc
    43621 ctcacccggt aggggcgagc gaggggaaaa caactcaccc gcgctcttgc atcaactgct
    43681 gggccagcgc cttcagttct ggcagatatt tttcatctac cggtccaaac ggtttgcggc
    43741 acagcggcac agaaacgaca tccatataat gcaggacagt tttcaggccg cggaatacgc
    43801 cagttttgat cagtaaatca atgactttgt tgcactcagt ttgcagtttc tgcgcggtct
    43861 ggatatcgcc ttctttcagc gccttcacga tcccctgata tcgccagccc atgatgttgt
    43921 aagtactgcc gataccacca tcagcgcctg ccagcaagcc ggaagcgaag atttcgtcgt
    43981 aaccgttata gagcaccaga tcaggatgtt cacgacggat ctgttccatc tgatagagat
    44041 cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt aaccagggtg ttgatctgat
    44101 ccagggtcag ttttacccca ctcagggctg gaatgttgta cacaaccatc ggcaaaccat
    44161 ccgctgaatc aataattgcc cgatagtgat cgcagtgttc ttcaaagctg aaaggatagt
    44221 agaacggcgt gacggcggag acggcatcga agccataacg tttagccgat gccgcaagtt
    44281 gttggctttc ggcggtgctg acgcaaccga cgtgggcgat gagtttaatc ttacctttcg
    44341 cctcttcggc gacgatttcc agtacctgtt cacgctcgga aaggctttgt acaaaggcct
    44401 cgccggtcga accacccacg tataaaccgt cgatgccctg ctgaatattg aactgaacca
    44461 ggcgacgcag actcgcttta tccagtgctt gttgttggtc aaaaggagtc aggagtgcag
    44521 ccattacgcc acgtaaattc gttgccataa atacctctga agtgatactt gtctgataaa
    44581 cgatatacct ttatacctgt tataccagat caattaagca acaccccata cagaaagctt
    44641 ataatgcgat ctgcttcact aaagtggcat tatttctttt tgttggtggt ctgaccgaaa
    44701 gcgtgccagg tagcagagac gctgttgaga tgcgattgca acgcacgatc ggcttcgtca
    44761 ggatcatgac ggcggatcgc atcaacgatc gcaatatgct gttgatagct aacgttgtta
    44821 tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg cgatcagcca gtcgagcagg
    44881 gcaacgtgga tcgccatgaa gattgggtta ccagggatct ccgccagcac gcggtggaaa
    44941 tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg actgactgtt gatttccagt
    45001 gcttttgcca gcaaatcgat ttgctcatcg gtggcatgtt cagccgcata gcgcaccaga
    45061 ctggattcaa agaacagacg taattgttcg aaatgggcaa tcccaccggg atgagaaagg
    45121 aaatctttcg ccatgccgga aaactcaccg atgatagtgt ccgcagaagg acgcgagacg
    45181 cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc gttttaacgc tgccagcgct
    45241 tcgcgcacag aaggacgccc gacgttaaag aacgccatca gttcgcgttc agacggtaat
    45301 tgttcacctt cgccaaattc acgacggcgg atcatctgtt ccagctcttc ttccaccatt
    45361 tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt tgcgaccaat tgcaggtgaa
    45421 gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca ttctgtaagg tcagtgtgat
    45481 taacatcatc agtgacatcc tatcacagga ttgaaagtag gggaaaatgg caggtttttc
    45541 tctttgtgcc tcatcattac cataattaac ggaataatta actattgcga aaaattaatg
    45601 taacgcagat aaacacatcc cgattgaatt attcataaga ctattcacaa gcattatgaa
    45661 tattatgaat gtgttcttac aaaataatca taagcgcata ttttttaatg aaaaatcaca
    45721 tcacctacaa ttaaaaacac gacatccgca ccataaatag acttgcaaaa aatataacat
    45781 cattgttttc aatctgccgt ttatgggatt gaccgttttc ttttgacacg gagttcaaca
    45841 atgttcggca taattatatc tgtcatcgta ttaattacga tgggctattt gatcctgaaa
    45901 aactacaaac ctcaggtggt gctggctgcc gcaggtgtct tcctgatgat gtgcggagtc
    45961 tggttagggt tcggtggtgt actcgatccc gccaaaagca gcggctactt gatcgtcgac
    46021 atttataatg aaatcctgcg catgctgtcc aaccgcattg ccggattggg gctgtcgatt
    46081 atggcggtgg gcggttatgc ccgctacatg gagcgcatag gagccagtcg cgcgatggtg
    46141 agcttgctaa gccgcccgtt aaaactcatt cgctcgccgt atattattct gtcggcaact
    46201 tacgtcatcg gccaaatcat ggcgcagttt attaccagcg cctccggtct gggtatgttg
    46261 ctgatggtca ccttatttcc gacgctggtg agtctgggag taagtcgcct ctctgcggtg
    46321 gcggttatcg caaccacgat gtccattgag tgggggattc tggaaacgaa ctccattttt
    46381 gcagcccagg tcgcgggaat gaaaattgcc acatacttct tccactacca gcttccggtc
    46441 gcctcttgcg tcattatctc ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac
    46501 aaaaaagata aaaatatcaa tcacgaacag gcagagcaaa aaactctcga taatgtcccg
    46561 ccgctctatt acgccatttt acctgtgatg ccgttaatcc tgatgctcgg ctcgctgttc
    46621 ctcgcccacg tcgggctgat gaagtcagaa ctgcatctgg tggtggtgat gttactaagt
    46681 ttgacggtga cgatgtttgt tgagttcttc cgcaagcata acttgcgcga aacaatggac
    46741 gatgtgcagg cgttttttga cggcatgggt acgcagtttg ccaacgtggt aacgctggtg
    46801 gtcgcgggtg aaatatttgc gaaaggctta acgacgattg gcactgttga tgcggttatc
    46861 aggggtgcgg agcattctgg tctgggcggt attggcgtga tgattattat ggcgctagtc
    46921 attgccattt gtgccattgt gatgggctct ggcaatgcgc cgtttatgtc atttgccagt
    46981 cttattccga atatcgcagc cggactacat gtaccagcgg ttgtaatgat tatgccgatg
    47041 cattttgcca cgacgctagc gcgcgcggtt tcgccgatta ctgcggtggt gaagcggaca
    47101 gcgatcccca tggcagtcgg tttcgtggtg aatatgattg ccacaatcac gctattttat
    47161 taagtcatta aaaagacaaa acaggccgcc tgggcctgtt ttgtattact tcacaacacg
    47221 taatgccggt cgaccaccgc gtggtggctg cggaggttca tcgtcaggat gagtgtcatc
    47281 atcgtgatct ggcttgtcgc catcaataac cgacataacg gtttcgttgt ctgccgatgc
    47341 ctcttcatca ttcatgatgc tggtatcttc atcgtaggca gcttcaggct caaacatcgt
    47401 gcctgcgcca ttttcacggg cgtagatagc cagcacggca gccagcggca cagaaacctg
    47461 acgcggaatg ccaccaaagc gcgcgttaaa gcgcacctca tcattcgcca gttccagatt
    47521 gccgacagca cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat attccatagg
    47581 aacctgcacg ccagggagcg tcacatccac caccaggtgc ggcgtgagct ggttatccag
    47641 caaccactca tagaatgcac gcagcagata gggacgacgt ggtgttagct gtgacaaatc
    47701 catacagatt aactccggcc cagacacatt tcacgttctg cttcagttaa agaagcaagg
    47761 aaagagtcac gctcaaagac gcgggtcata tagcctttca gctctttcgc acccgggccg
    47821 ctgagctcga tacccagttg cggcagacgc cacagcagcg gagcaagata gcaatcgacc
    47881 aggctgaact catcgctcag gaagtacggc ttctgaccga agaccggcgc aatcgccagc
    47941 agttcttcgc gcagttgctt acgtgcggca tctgcttcag aagctgaacc gttgatgatg
    48001 gtgttcatca gcgtgtacca gtctttttcg atgcgatgca tgtacagacg gctttcaccg
    48061 cgagctaccg ggtaaacagg catcagtggc ggatgcggga aacgctcatc cagatattcc
    48121 ataatgatgc gagattccca cagggtcagc tcacgatcca ccagggtcgg aacgctctga
    48181 ttcgggttga ggtcaatcag atcctgaggc ggattgtcct tttccacgtg ttcgatctcg
    48241 aaacttacac ctttctcagc cagcacaatg cggacctgat ggctatagat gtcagtagga
    48301 ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga cagccatgaa aacctccagg
    48361 tatagtcaga atttttactg ctaccagcca ccaggtggcc agtcagaagt tgtgttaccc
    48421 aataaggaac gactctcttt gttcgaaaat caaacaaaaa atgagcaata cccgacattt
    48481 gggcagaaaa ttggatgata gtttaccaga ttttgcgacc attgtggtga gtggatgccg
    48541 gaaatgggga aaaagagatg cgctttagtc tgaaatagtt gacttagacc cttattggcg
    48601 atgtggtttt tgttttgcct gtctttcaga cggcagcaaa aagcaacttc ccatttttta
    48661 cgctgattca gaatttagcc ataaaaaaac ccgccgaagc gggttttttc gtaaattgtt
    48721 ttctgccgga gcagaagcca attaacgttt ggagaactgc ggacgacgac gtgctttacg
    48781 cagaccgact ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg
    48841 cagttcagaa cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat
    48901 cgcaccagcc tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc
    48961 aaccatgtcg accagttcca gcggctgacg aactaccatg cgggcagttt cacgaccgaa
    49021 gtactgttcc agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac
    49081 gcgagctgcg gaatttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta
    49141 taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt gctcgttacc
    49201 cgcgtaaact ttcagtttac ggaacatagc acgacccagc gggccttttg gcaacatgcc
    49261 tttaaccgcg atttcaatca cacgctcagg acggcgagca atcatctctt caaagctcac
    49321 ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac actttgtcag tacgcttgtt
    49381 gccggttaca gcaactttgt cagcgttcag aacgatgatg taatcaccgg tatctacgtg
    49441 cggagtgtat tccgctttgt gcttaccgcg taggcgacga gccagttcag tagccagacg
    49501 gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg tttctggttt
    49561 agctgtaaaa gttttcatta aaagcttacc caataaatag ttacacgttg gtgaacaccc
    49621 aaacgtcttc aattgttgag gttcacacga caaagtccgg caaacctacc ccttcgaata
    49681 gcctatgcca gcacacaaaa agttttggga aaaaaacttt cttgtaacgt ggggtcgcag
    49741 gattatagag aagtcggggt caaagatcga cccctttttg tgatttgtga caggttttaa
    49801 cccgccaaat gctcgcgctt cagatactct tcgctttgca tctcttgcag acgtgacagg
    49861 caacgctgga actcaaactt cagccgatcg ccctgataaa tttcatacag cggcacttct
    49921 gcactcacca ctaatttgac atggcgctcg taaaactcat ccaccagcgc aataaagcgc
    49981 cgcgcttcgc tctccatcaa ccgcgtcata actggtacat caaacaacat gaccgtatga
    50041 aagagacgtg agagcgcaat atagtcatgc tgactgcggg cgtcgacgca cagcgtagta
    50101 aaagagaccg ccagcgtctg gttctcgacg cccattgtcg ctaatggccg atggttgatt
    50161 tctaacgtcg gtgaattttc tcgtttcccc cccgccagcg ccaaccatag tttatccatt
    50221 tgcgcccggg tttcatcgtg gagtggcgaa agccacagat gcgcctgagt gagtgtacgc
    50281 agtcgataat caacaccagc gtccacgttc attacatcac aatgctgttt aatggcatcg
    50341 attgcaggca gaaaacgcgc acgttgcaag ccatttcgat aaagttcgtc cggcggaata
    50401 tttgacgtcg ctaccagggt aataccgcgg gcgaacaagg ctttcatcag accgccaagt
    50461 agcatggcat cggtaatatc agaaacaaaa aattcgtcaa aacagagcac gtcagtttcg
    50521 gctttaaagc gatcggcaat aatttccagc ggatcggtct gcccctgtaa ggcagttagc
    50581 tcttcatgca cccgcagcat aaaacggtga aagtgcaggc gctgtttccg ctctcccggc
    50641 aggctttgat agaaaaggtc catcagccag gttttcccgc gtcctacacc gccccacata
    50701 tataagccac gcactggcgt atgctttgtg tcttcgcgtt taccccacag cttaccgacc
    50761 cgcgccatta gcccactcgt cctgggggct ggtggcgtgc tattgatgag ttcctgataa
    50821 ataatttcca ggcggctgac ggcctctttt tgaacgtcgt cgggttgatg gctgccttca
    50881 ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt gcatgatctt attgttattc
    50941 cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa aaaggccgtt ctacactacg
    51001 cgatatgcag tcgggattcc acttctgtgg aattaacggt tatagtggca taatcagccg
    51061 caggcatgga gcctgaagcc aacaccctac ggaaacaaaa gacaacggga gatgttcatg
    51121 acctgggaat atgcgctaat tgggttagtc gtcggcatca ttattggtgc tgtggccatg
    51181 cgttttggta atcgtaaact acgccagcaa caggcgttgc agtacgaact ggaaaagaat
    51241 aaagctgaac tggacgagta tcgcgaagag ctggttagcc actttgcccg cagcgcggaa
    51301 ttactggata ccatggcgca cgactatcgc cagctgtatc agcacatggc gaaaagttcc
    51361 agcagcctgc tgccggaact gtctgctgaa gcaaacccgt tccgtaatcg tctggcagag
    51421 tctgaagcca gcaacgatca ggcaccggtg cagatgcctc gcgactattc tgaaggcgca
    51481 tccggcctgc tgcgtactgg cgcgaagcgc gactaattta tttttcgggc gcagccattg
    51541 cgccctcctc ttctctccct ccccgactat catttaatct ggtgtctcat tgttagccgt
    51601 ctgaaaattc aataacatca aactgttttg aatctctttt cttatcattc aggtacgaga
    51661 gcaggaataa tgaaaaaaca accccagctg ttgagtgcat tagcgttaag tgtcgggtta
    51721 actctctcgg cgtcatttca ggccgtcgcg tcgattccag gccaggttgc cgatcaggcc
    51781 cctctcccca gtctggctcc aatgctggaa aaagtgcttc cggcagtggt gagcgtacgg
    51841 gtggaaggaa cggccagtca gggacagaaa atcccggaag aattcaaaaa gttttttggt
    51901 gatgatttac cggagcaacc tgcacaaccc ttcgaaggtt taggctccgg tgtcatcatc
    51961 aacgccagta aaggctatgt gctgaccaac aaccatgtga ttaatcaggc acagaaaatc
    52021 agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac tgattggtag cgatgaccag
    52081 agcgatatcg ccctgttaca aattcaaaac ccgagcaaat taacgcaaat cgctattgcc
    52141 gactccgata aattgcgcgt cggtgatttt gccgtagcgg tcggtaaccc atttggcctt
    52201 gggcaaaccg ccacctctgg cattgtttcc gcattaggac gcagcgggtt gaatcttgaa
    52261 ggtctggaaa actttatcca gacagatgct tccattaacc gcggtaactc cggcggtgca
    52321 ctgttaaacc ttaacggtga gttaattggc atcaacactg caatccttgc gcctggcggc
    52381 gggagcgtcg ggattggatt tgccatcccc agtaatatgg cgcgaacact ggcgcagcag
    52441 cttatcgact ttggtgaaat caaacgcggt ttgttaggca tcaaaggcac cgagatgagt
    52501 gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg gcgcgtttgt cagcgaagtg
    52561 ttgccaggtt ctggctcggc gaaagcgggc gtcaaagcgg gcgatattat taccagcctc
    52621 aacggcaaac cgctgaatag ctttgctgag ttgcgctctc gtatcgcgac caccgagccg
    52681 ggcacaaaag tgaaacttgg cctgctgcgt aacggcaaac cactggaagt agaagtgacg
    52741 ctcgatacca gcacctcttc gtcggccagc gctgaaatga tcgcgccagc gctggaaggt
    52801 gcaacgttga gcgatggtca gctaaaagat ggcggcaaag gtattaagat cgatgaagtt
    52861 gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag acgatgtgat cattggcgtc
    52921 aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag tgctggcggc aaaatcggcc
    52981 atcatcgccc tgcaaattgt acgcggcaat gaaagcatct atctgctgat gcgttaatgt
    53041 cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa ctcgtggtat gctgctgccg
    53101 ttcccttttt taatgacgcc tccatcatgt ttgtgaagct cttacgttcc gttgcgattg
    53161 gattaattgt cggcgctatt ctgctggttg ccatgccttc gctgcgcagc cttaacccgc
    53221 tttccactcc gcaatttgac agtaccgatg agacgcctgc cagctataat ctggcggttc
    53281 gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg tttgaacacc aactctcaca
    53341 accagcttga gatccgcacc ctgggatccg gtgtaatcat ggatcaacgc ggttatatca
    53401 tcaccaataa acacgtcatc aacgacgccg atcagatcat cgtcgcctta caggatggtc
    53461 gtgtatttga agcattgctg gtgggatctg actctctaac cgatctggcg gtacttaaaa
    53521 ttaatgccac tggcggttta cctaccattc caatcaatgc acgtcgcgta ccgcatattg
    53581 gcgacgtggt actggcgatc ggtaacccgt acaacctcgg gcagaccatt acccagggga
    53641 ttattagtgc cacgggtcga attggtctga acccgaccgg gcggcaaaac ttcctgcaaa
    53701 ccgatgcttc cattaaccac ggtaactctg gcggcgcgct ggtgaactcg ctgggcgaac
    53761 tgatgggcat taacacgctg tcgtttgata agagtaacga tggcgaaacg ccggaaggta
    53821 tcggctttgc gattcctttc cagttagcaa ccaaaattat ggataagctg atccgcgatg
    53881 gtcgcgtgat ccgcggctac attggcattg gcggacgtga gatcgcaccg ctgcacgcac
    53941 agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa tgaagtgtca cctgacggcc
    54001 cggcggcgaa tgcgggtatt caggtcaacg atctgattat ttcggtggat aacaaaccgg
    54061 ccatctctgc tctggagacg atggatcagg tggcggaaat tcgccctggt tcggtgatcc
    54121 cggttgtagt gatgcgtgat gataagcagt taacgctgca ggtcaccatt caggaatatc
    54181 cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg agtctgtg
//
LOCUS       DAZOZD010000002        49499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOZD010000002 DAZOZD010000000
VERSION     DAZOZD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.002"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            49..681
                     /gene="comR"
                     /locus_tag="ACHUPB_000059"
     CDS             49..681
                     /gene="comR"
                     /locus_tag="ACHUPB_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0756992.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(743..1282)
                     /locus_tag="ACHUPB_000060"
     CDS             complement(743..1282)
                     /locus_tag="ACHUPB_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0756993.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1509..2813)
                     /gene="ndh"
                     /locus_tag="ACHUPB_000061"
     CDS             complement(1509..2813)
                     /gene="ndh"
                     /locus_tag="ACHUPB_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0756994.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3221..3763)
                     /gene="ycfP"
                     /locus_tag="ACHUPB_000062"
     CDS             complement(3221..3763)
                     /gene="ycfP"
                     /locus_tag="ACHUPB_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0756995.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3786..4811)
                     /gene="nagZ"
                     /locus_tag="ACHUPB_000063"
     CDS             complement(3786..4811)
                     /gene="nagZ"
                     /locus_tag="ACHUPB_000063"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0756996.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4822..5646)
                     /gene="thiK"
                     /locus_tag="ACHUPB_000064"
     CDS             complement(4822..5646)
                     /gene="thiK"
                     /locus_tag="ACHUPB_000064"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0756997.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5627..6268)
                     /gene="lpoB"
                     /locus_tag="ACHUPB_000065"
     CDS             complement(5627..6268)
                     /gene="lpoB"
                     /locus_tag="ACHUPB_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0756998.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6282..6659)
                     /locus_tag="ACHUPB_000066"
     CDS             complement(6282..6659)
                     /locus_tag="ACHUPB_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0756999.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6662..7021)
                     /gene="hinT"
                     /locus_tag="ACHUPB_000067"
     CDS             complement(6662..7021)
                     /gene="hinT"
                     /locus_tag="ACHUPB_000067"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0757000.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7355..9544
                     /gene="fhuE"
                     /locus_tag="ACHUPB_000068"
     CDS             7355..9544
                     /gene="fhuE"
                     /locus_tag="ACHUPB_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR0757001.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9604..11037)
                     /gene="ptsG"
                     /locus_tag="ACHUPB_000069"
     CDS             complement(9604..11037)
                     /gene="ptsG"
                     /locus_tag="ACHUPB_000069"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0757002.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11332..12129)
                     /locus_tag="ACHUPB_000070"
     CDS             complement(11332..12129)
                     /locus_tag="ACHUPB_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0757003.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12140..13144)
                     /gene="holB"
                     /locus_tag="ACHUPB_000071"
     CDS             complement(12140..13144)
                     /gene="holB"
                     /locus_tag="ACHUPB_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0757004.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13141..13782)
                     /gene="tmk"
                     /locus_tag="ACHUPB_000072"
     CDS             complement(13141..13782)
                     /gene="tmk"
                     /locus_tag="ACHUPB_000072"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0757005.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13772..14794)
                     /gene="yceG"
                     /locus_tag="ACHUPB_000073"
     CDS             complement(13772..14794)
                     /gene="yceG"
                     /locus_tag="ACHUPB_000073"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0757006.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14797..15606)
                     /gene="pabC"
                     /locus_tag="ACHUPB_000074"
     CDS             complement(14797..15606)
                     /gene="pabC"
                     /locus_tag="ACHUPB_000074"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0757007.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15726..16967)
                     /gene="fabF"
                     /locus_tag="ACHUPB_000075"
     CDS             complement(15726..16967)
                     /gene="fabF"
                     /locus_tag="ACHUPB_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0757008.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17055..17291)
                     /gene="acpP"
                     /locus_tag="ACHUPB_000076"
     CDS             complement(17055..17291)
                     /gene="acpP"
                     /locus_tag="ACHUPB_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0757009.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17502..18236)
                     /gene="fabG"
                     /locus_tag="ACHUPB_000077"
     CDS             complement(17502..18236)
                     /gene="fabG"
                     /locus_tag="ACHUPB_000077"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0757010.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="ACHUPB_000078"
     CDS             complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="ACHUPB_000078"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0757011.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19194..20147)
                     /gene="fabH"
                     /locus_tag="ACHUPB_000079"
     CDS             complement(19194..20147)
                     /gene="fabH"
                     /locus_tag="ACHUPB_000079"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0757012.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20215..21285)
                     /gene="plsX"
                     /locus_tag="ACHUPB_000080"
     CDS             complement(20215..21285)
                     /gene="plsX"
                     /locus_tag="ACHUPB_000080"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0757013.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21366..21539)
                     /gene="rpmF"
                     /locus_tag="ACHUPB_000081"
     CDS             complement(21366..21539)
                     /gene="rpmF"
                     /locus_tag="ACHUPB_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0757014.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21591..22112)
                     /gene="yceD"
                     /locus_tag="ACHUPB_000082"
     CDS             complement(21591..22112)
                     /gene="yceD"
                     /locus_tag="ACHUPB_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0757015.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22311..22895
                     /gene="yceF"
                     /locus_tag="ACHUPB_000083"
     CDS             22311..22895
                     /gene="yceF"
                     /locus_tag="ACHUPB_000083"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0757016.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23007..23966)
                     /gene="rluC"
                     /locus_tag="ACHUPB_000084"
     CDS             complement(23007..23966)
                     /gene="rluC"
                     /locus_tag="ACHUPB_000084"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0757017.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24539..27724
                     /gene="rne"
                     /locus_tag="ACHUPB_000085"
     CDS             24539..27724
                     /gene="rne"
                     /locus_tag="ACHUPB_000085"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0757018.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27919..28872)
                     /gene="flgL"
                     /locus_tag="ACHUPB_000086"
     CDS             complement(27919..28872)
                     /gene="flgL"
                     /locus_tag="ACHUPB_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR0757019.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28884..30527)
                     /gene="flgK"
                     /locus_tag="ACHUPB_000087"
     CDS             complement(28884..30527)
                     /gene="flgK"
                     /locus_tag="ACHUPB_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR0757020.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30593..31534)
                     /gene="flgJ"
                     /locus_tag="ACHUPB_000088"
     CDS             complement(30593..31534)
                     /gene="flgJ"
                     /locus_tag="ACHUPB_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0757021.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31534..32631)
                     /gene="flgI"
                     /locus_tag="ACHUPB_000089"
     CDS             complement(31534..32631)
                     /gene="flgI"
                     /locus_tag="ACHUPB_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0757022.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32643..33341)
                     /gene="flgH"
                     /locus_tag="ACHUPB_000090"
     CDS             complement(32643..33341)
                     /gene="flgH"
                     /locus_tag="ACHUPB_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0757023.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33394..34166)
                     /gene="flgG"
                     /locus_tag="ACHUPB_000091"
                     /pseudo
     CDS             complement(33394..34166)
                     /gene="flgG"
                     /locus_tag="ACHUPB_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHUPB_000092"
     CDS             complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHUPB_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR0757024.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHUPB_000093"
     CDS             complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHUPB_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0757025.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHUPB_000094"
     CDS             complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHUPB_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0757026.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHUPB_000095"
     CDS             complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHUPB_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR0757027.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHUPB_000096"
     CDS             complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHUPB_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0757028.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38029..38688
                     /gene="flgA"
                     /locus_tag="ACHUPB_000097"
     CDS             38029..38688
                     /gene="flgA"
                     /locus_tag="ACHUPB_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0757029.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38764..39057
                     /gene="flgM"
                     /locus_tag="ACHUPB_000098"
     CDS             38764..39057
                     /gene="flgM"
                     /locus_tag="ACHUPB_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0757030.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            39062..39478
                     /gene="flgN"
                     /locus_tag="ACHUPB_000099"
     CDS             39062..39478
                     /gene="flgN"
                     /locus_tag="ACHUPB_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0757031.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHUPB_000100"
     CDS             complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHUPB_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0757032.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41163..42086)
                     /locus_tag="ACHUPB_000101"
     CDS             complement(41163..42086)
                     /locus_tag="ACHUPB_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0757033.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42088..42735)
                     /locus_tag="ACHUPB_000102"
     CDS             complement(42088..42735)
                     /locus_tag="ACHUPB_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR0757034.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHUPB_000103"
     CDS             complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHUPB_000103"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0757035.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHUPB_000104"
     CDS             43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHUPB_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0757036.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44838..45485
                     /gene="grxB"
                     /locus_tag="ACHUPB_000105"
     CDS             44838..45485
                     /gene="grxB"
                     /locus_tag="ACHUPB_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0757037.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45619..46179
                     /locus_tag="ACHUPB_000106"
     CDS             45619..46179
                     /locus_tag="ACHUPB_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0757038.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHUPB_000107"
     CDS             46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHUPB_000107"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0757039.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47405..47650
                     /gene="dinI"
                     /locus_tag="ACHUPB_000108"
     CDS             47405..47650
                     /gene="dinI"
                     /locus_tag="ACHUPB_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0757040.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47940..48194
                     /gene="bssS"
                     /locus_tag="ACHUPB_000109"
     CDS             47940..48194
                     /gene="bssS"
                     /locus_tag="ACHUPB_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0757041.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48309..49427
                     /gene="solA"
                     /locus_tag="ACHUPB_000110"
     CDS             48309..49427
                     /gene="solA"
                     /locus_tag="ACHUPB_000110"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0757042.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        12023 a        13338 c        12279 g        11859 t
ORIGIN      
        1 tatttttata ataacctttc attcacagtc accaggttca acggcatcat ggcaactgac
       61 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
      121 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
      181 ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
      241 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
      301 cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc
      361 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
      421 accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa
      481 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
      541 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
      601 gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg ccggaacgac tttgcgttta
      661 tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc
      721 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccgttactat
      781 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
      841 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
      901 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
      961 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccacga ccaccaccaa
     1021 actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt
     1081 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
     1141 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccccgttcaa
     1201 acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac
     1261 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
     1321 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata
     1381 cttaaggatt cggagaagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag
     1441 gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg
     1501 tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc
     1561 atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag
     1621 atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc
     1681 atcaggctac cgacagtgga gaagttcgac agcgatacca gcgaaccatg atctttatac
     1741 tgataatttt tcagcggttt accgttcatc tgcgccagaa tatttttcat tgcgcaggtc
     1801 gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat
     1861 gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc
     1921 accaccagct ggttgatacg gttggtttca agaccaccga tatctttcag gaagtctggc
     1981 gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcacc atctttagtg
     2041 tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt
     2101 ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt
     2161 tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag
     2221 ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga gttctacacc cgtcgcgccg
     2281 ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca ggttggcgga gtatttcagg
     2341 aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg
     2401 cagttctctt tgacacctgg cgtattgaaa tcgttagagg tgctacccag cgccattacc
     2461 agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt
     2521 tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc cagctggaac
     2581 tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc
     2641 gagccagtcg ccacttcgtg cagcagcggt ttccacagat ggctgtggtt acgatcgacc
     2701 agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc
     2761 agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt caacgtgacc
     2821 cccttaaatt tattaaccaa ttgttaatta atggttataa acatagctct taattaacaa
     2881 caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc
     2941 ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact ttaccgggtg
     3001 agatcaaaaa acagagttta ttgtttatta tttgagcaag tccggaaggg tgatataggg
     3061 tgacgcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca
     3121 ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc gacttacaga attcggagcg
     3181 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
     3241 ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
     3301 agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta agcgcttcat
     3361 cattacgcga caaaatcacc aggcaacgat cgcgattctt ctcgcggaag ttggtcacac
     3421 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
     3481 aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt
     3541 ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg acgttcagtt
     3601 gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga tgccgcgtac
     3661 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
     3721 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
     3781 cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg
     3841 ctgatcgcct tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
     3901 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
     3961 cctttacgat tattgcagac caggatcata tcgcaacctg cgtccagtga tgcctgcccg
     4021 cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatt
     4081 acaccgtcaa aacacagttc ctgacgcaaa acggttttca gccagtagga agaaccgctc
     4141 gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
     4201 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga
     4261 cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
     4321 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
     4381 gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc
     4441 ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc
     4501 atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca
     4561 aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct
     4621 tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt
     4681 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
     4741 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
     4801 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
     4861 cagcctgata aattgttgat cgccgatttg tcgccagcgg tactcaaacc accctgcttt
     4921 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
     4981 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca
     5041 caccgccgcc agttccagcg cgatatcacc atctccggca tactcccagt cgatgagttt
     5101 taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
     5161 gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac
     5221 agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat
     5281 tcgccagcca aaacgtggtt gttgatgaag ataatacagc aagcctgcca gttcgttggt
     5341 atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag
     5401 atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg
     5461 caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
     5521 ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg
     5581 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
     5641 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta
     5701 gggtcggagc cttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat
     5761 tgcgggcaat gcctatggct ttactacggg tacctaaact gtcctgcggc gacaaaccta
     5821 actgttgctt agccatcgac agctgctggg cggaaaccag ggtaaatttc ccgttattag
     5881 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
     5941 tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct
     6001 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
     6061 gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg
     6121 gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt
     6181 ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
     6241 tcaaggcgta gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt
     6301 ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg
     6361 ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac
     6421 cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat
     6481 gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc
     6541 aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa
     6601 cgacagccca ccagcagtaa caacgccaga gacaccagcc caaagcatcc ttttctcatc
     6661 gttacagacc tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat
     6721 ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat
     6781 cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg
     6841 cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca
     6901 gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt
     6961 agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca
     7021 cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg
     7081 agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc
     7141 aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc
     7201 tgaaaagaca ttctctgttt ggtttacacg acaaatatga atgcgcatat ttctcatttg
     7261 catttacaaa caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
     7321 gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga
     7381 taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact
     7441 attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
     7501 agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac
     7561 caaaatgcag atgactcaac ttgatattcc tcagtcggtc actattgtta gccagcagcg
     7621 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
     7681 caaaagtcag gcggattccg atcgtgctct ttattattcc cgtggattcc agatcgataa
     7741 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
     7801 ttctgatatg gcactctttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
     7861 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga
     7921 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
     7981 tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca
     8041 gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct cgggcattgt
     8101 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
     8161 tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag
     8221 ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa
     8281 ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
     8341 ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc
     8401 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg
     8461 caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag
     8521 ttatgaattg tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa
     8581 caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa
     8641 ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcac aggacgatac
     8701 cacacatatg aaatcgttat atgctgctac tcgagtcacc cttgccgatc cgctgcatct
     8761 gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa
     8821 aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta
     8881 cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct
     8941 ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg
     9001 tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg
     9061 tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag
     9121 taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg
     9181 cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg
     9241 caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg
     9301 cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt
     9361 ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa
     9421 aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacat
     9481 ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt
     9541 ctgaataaca cctgtaaaaa aggcagccat ctggcggcct tagtctcccc aacgtcttac
     9601 ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc
     9661 gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag
     9721 gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc
     9781 gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc
     9841 gctggtacct gttgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc
     9901 cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac
     9961 gatcgggaac agccacagtt tgctgctgtt accagacaga acgataaagt cgatcagacc
    10021 gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc
    10081 tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg agaactcgat
    10141 cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac
    10201 tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc
    10261 gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata
    10321 acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat
    10381 ctggaaaggt acgttccaga tgtggtgcag accaaacggc accaggcaac gttcgatgaa
    10441 accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg
    10501 gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc
    10561 caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag
    10621 cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga taatccctcc
    10681 gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta aatgcagtac
    10741 cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag
    10801 cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg
    10861 catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag
    10921 ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca gtacggatac
    10981 cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct taaacataat
    11041 tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca aggggggaga
    11101 gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc
    11161 agtgccgatt tgtttgacgg ctatcacatt tcaacttcag atcattggag aagttgcaca
    11221 ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc aattttcggc
    11281 ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat
    11341 ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg
    11401 cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac
    11461 catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt
    11521 ttccaccagt aaccgatcca gggggacata acgcgcagca tctcgaagtt gctccgcatt
    11581 acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc
    11641 cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc
    11701 gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta tggacgataa ccggcttgtt
    11761 cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgaa ctttagtttc
    11821 cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc
    11881 cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca
    11941 agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat aacccggtaa
    12001 tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc
    12061 atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac
    12121 taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt
    12181 aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag
    12241 acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag
    12301 aaagatggtt tgccagttcg acgacaaggc ccggcacatc aacattggtc acctgcgcag
    12361 caccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg
    12421 gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat
    12481 atgccaatgc ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca
    12541 gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca
    12601 ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc gcaaggtaat
    12661 gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga
    12721 aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt
    12781 cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg cgtgcgtgct
    12841 cattcagctt ttcggtgacc tcacgtactg catcaatgcc cagcgtattt ttcccttttt
    12901 cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac
    12961 agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt
    13021 agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc
    13081 ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc
    13141 tcatgcgtcc aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc
    13201 ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag ccagttccag
    13261 atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc
    13321 gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa
    13381 gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat
    13441 accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca
    13501 ggtgccgtta gccagagctg gtttgatgac cgtttctacc agttgaacgc gcgcggcata
    13561 aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag
    13621 caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat
    13681 gtcgcggata cccagttgct caagcgtctc aaccaccaca ttacgcgcgg tagttttgcc
    13741 tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt
    13801 tcagataatc ctgcacagac ttgttatggc tggcaagatt ggtattaaac gtgtgaccac
    13861 ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca
    13921 gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt
    13981 tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca
    14041 tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa
    14101 ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg
    14161 ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg
    14221 cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg acatcggtgg
    14281 tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt
    14341 ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg tgcttgatat
    14401 acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca
    14461 gcgggaactg tgcttcttta ccgctttcca gcaatttcag catctcgcgc acggtcatct
    14521 gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca
    14581 accattgaaa aacccgtggg cgattgatga tcttatcggc ataaagctgt tcaccaagcg
    14641 ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc
    14701 tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca
    14761 gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg
    14821 ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg cacgtacggg
    14881 catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg cctcttccag
    14941 agaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga tacagaattg
    15001 tcgcataatg ccgttaacac ctgcctgatc cagacgcggc gtataaacta cattgccctt
    15061 ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag
    15121 cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct caagccgatt
    15181 aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa
    15241 cgtcatccct tcgttacgca aacggtcgta atgtgcagga taaaccgtaa cggagagaat
    15301 ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat
    15361 cacgactttc agcacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag
    15421 ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat
    15481 atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc
    15541 accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttatgac cgttaattaa
    15601 gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa
    15661 gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtgggaaatg
    15721 acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta
    15781 cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg
    15841 caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc
    15901 aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat
    15961 ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc
    16021 gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga
    16081 ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg ccagagctgc gcctgcgcca
    16141 ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca
    16201 gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg
    16261 gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc
    16321 gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac acccagcggc
    16381 gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata
    16441 cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt
    16501 gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac
    16561 ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct
    16621 tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc
    16681 tcttccgtta tatcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat
    16741 tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa
    16801 tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg
    16861 ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca
    16921 ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccag
    16981 ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    17041 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    17101 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    17161 accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg
    17221 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    17281 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    17341 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    17401 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    17461 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    17521 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    17581 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata
    17641 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    17701 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    17761 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    17821 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    17881 tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca ctcttcatct
    17941 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    18001 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    18061 ttcaacatca gacctttgcc gttggcacct aaataatcgc tgatcgcctg agcgccattt
    18121 tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    18181 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt
    18241 ttcctctttt aaagctcaag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    18301 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg gccgacttca
    18361 tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg
    18421 ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca
    18481 tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc taattctact
    18541 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    18601 ggcagagcac gttttgcgcc cgcagcttta caggcagcgc cagcacgctc aaccgcttct
    18661 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    18721 cctgctgcag cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc
    18781 gccatagcgc ccgtaccttc cggtacggct tcttgcatga gcttgccgcg catctcaacc
    18841 agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga atattccccc
    18901 aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcggtac
    18961 agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    19021 gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    19081 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    19141 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    19201 cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    19261 cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca
    19321 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat accgagtttt
    19381 ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    19441 gaacggtcaa gattatttgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc
    19501 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcaca
    19561 cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg cagatgggtg
    19621 gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    19681 ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    19741 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    19801 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccagcatg
    19861 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    19921 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    19981 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    20041 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    20101 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    20161 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    20221 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    20281 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    20341 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    20401 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    20461 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    20521 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta atgtgacatt
    20581 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    20641 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    20701 actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg
    20761 aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt
    20821 actgtcacaa tcgacgttgg cccctaagtc aaggaccacc gttttgccct tttgctgatg
    20881 tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    20941 tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    21001 tttcaccagc tccagggcca cgcgcattga actcccacga ctggcgcgga tagcttgcga
    21061 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    21121 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    21181 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    21241 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    21301 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    21361 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    21421 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    21481 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    21541 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    21601 tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg cagttcacca
    21661 aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    21721 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    21781 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    21841 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    21901 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    21961 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    22021 acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    22081 tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    22141 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    22201 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    22261 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    22321 ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt
    22381 ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt
    22441 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    22501 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    22561 cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata
    22621 ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg
    22681 acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaggagcatc
    22741 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt
    22801 tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    22861 tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgctggat gcggttacgt
    22921 cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc aggccaaccg
    22981 cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg caaacaacgc
    23041 ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc cggatgggta
    23101 aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc
    23161 tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatac
    23221 tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg caccagggtg
    23281 gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactg
    23341 acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc ttgaacgctc
    23401 ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg cattcctttt
    23461 tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctat cagcaacaca
    23521 cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc cgggcgcaac
    23581 gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg
    23641 gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc
    23701 gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt
    23761 gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc
    23821 acccgcactt cgcctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat
    23881 tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt
    23941 tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg
    24001 ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact acgtgatcac
    24061 tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg
    24121 tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt
    24181 tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga
    24241 tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg
    24301 ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt
    24361 gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag
    24421 ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg
    24481 aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat
    24541 gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg
    24601 gcagcgtctt tatgacctgg atatcgaaag tccagggcac gagcagaaaa aggcaaacat
    24661 ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg
    24721 cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa
    24781 ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat
    24841 tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag
    24901 tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg
    24961 ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc
    25021 ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca
    25081 atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg
    25141 cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcat tccgcgatta
    25201 cttacgtcag gacatcggcg aaatccttat cgataacccg aaagtgctcg aactggcacg
    25261 tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtataccgg
    25321 cgagatcccg ctgttcagcc actaccagat tgagtcgcag atcgagtccg ccttccagcg
    25381 tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac agcaccgaag cgttaacggc
    25441 catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ccgcgtttaa
    25501 caccaacctc gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg
    25561 cctgattgtt atcgacttca tcgacatgac gcctgtacgc caccagcgtg cggtagaaaa
    25621 ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg
    25681 ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg aatccagcca
    25741 tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct
    25801 ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc
    25861 cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg cggtgaatgc
    25921 gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac
    25981 cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct taagctacat
    26041 gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg
    26101 taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtgc cgccagcgcc
    26161 aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg caacggcaac
    26221 accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct
    26281 gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa
    26341 accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga
    26401 gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg
    26461 taatcgtcgc caggcacagc agcagactgc cgagacgcgt gagagccgtc agcaggctga
    26521 ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg
    26581 ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga
    26641 gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa
    26701 acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt
    26761 cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc
    26821 acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca
    26881 agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc
    26941 gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc
    27001 aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa
    27061 agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag agcagcgcga
    27121 acaggaagaa gtacaagtgc agccgatggt gactggggtc cctgtcgccg ccgctgtcga
    27181 acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa gttgtagaag cccccgttca
    27241 gattgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc
    27301 agtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagttgc
    27361 agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc
    27421 cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt
    27481 cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg gttgaacctg aggtcaccgt
    27541 tgagcataac cacgctacgg tgccgatgac gcgcgctccg gcaccggaat atgttccgga
    27601 tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc
    27661 aggtggtcat acggcaacac atcatgcctc tgccgctcct gcgcgtccgc aacctgttga
    27721 gtaataatta gctcaagtaa tcaagccctg gtaactgcca gggctttttt atttcatctt
    27781 tgaatcaaca tattaaaatt catcgaatgg catccttgct aaccaacaat gcaaaatagg
    27841 caaaaaaatg cccgccccgg cgatcccaga gcaggcagac aaaacatacc cagtttcatg
    27901 atatgtttta aagcgaaatt atttgttgag ctggaagagc gacaatccct gcatatcggt
    27961 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc
    28021 attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct
    28081 acctaatgaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacggt
    28141 cagcacattg ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg ccgcagcggc
    28201 ggtttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc
    28261 cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact
    28321 ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc
    28381 gacctgttgc ttaatacttt ctgttccgcc gacataatca ccgttagcct cactaaacgg
    28441 cgcagtctct gttttataac cggcaaaaat gtagcgccca ttaccgtcag tggtgttcgc
    28501 cagattcagc aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc
    28561 gtcactcaag gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt
    28621 ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc
    28681 cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt gtgatgcagc
    28741 aatgggatca tctgaagggt taacgactcg cttacccgtc gacatctgtt cgccgtactt
    28801 catccattct gcctgagaat tggtgatacc acgcatgttt tgctggtaca tcatctgggt
    28861 actgaaacgc attttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt
    28921 gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg
    28981 tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc
    29041 acattacctt gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc
    29101 actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actattgctt
    29161 tgcagatcca gcagggcctg accgttgcgg ttatcgctat cacccgcatc tttttcgctc
    29221 gccatcgcta ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt
    29281 actggtttca gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga
    29341 ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg taaaagtggt attgctggca
    29401 aggcgggtga cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc
    29461 acggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc
    29521 agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag catcgccgtt agcatcaaat
    29581 ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta
    29641 ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta
    29701 ttcagtaatt tctccgggat ctcaatattg cctgccgtcc catcaacata agcgacagtc
    29761 gtacgagaag ggtcagcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga
    29821 accagtgagt aaccattggc caccgtgatg ttataagtgc cgccatcctg aacgctgact
    29881 tctacaccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    29941 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    30001 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    30061 ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat
    30121 ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg
    30181 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    30241 tcgattttcg acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca
    30301 cgtaactggt tggtaataaa cgcatcatac tcacgctgca caccagaaac gtagacgcca
    30361 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    30421 cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    30481 tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat
    30541 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    30601 gattatcaat gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct
    30661 ggatcatgtt ggtgagttta cgggcatagt gaggatcggt agcatacccc gcgtcctgta
    30721 gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac ggcggcgtag cgcgggttac
    30781 gtgttaacag cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaatt
    30841 tcgcttttac tttcttcgct tcgccatttt catattcagt cgtggtgatt tcagtaactg
    30901 gacctttcca gttgccagag gctttgacgc caaacaggtt atagctcggc tcgccgtttt
    30961 cgcggcggat ttgccgttgc ccccaaccag actccagcgc cgcctgagcg aggatcaaat
    31021 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    31081 atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca
    31141 gctgcgaaag cgcctgattt tgataacgca ccacagtttc gagcgggaat ttcatcggtg
    31201 ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    31261 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatctgctgg tcatacatac
    31321 tggtatacag gcgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    31381 tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat
    31441 ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg
    31501 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    31561 acatcccgca ctttgcattg attgcagtat ggacatcaga tccatcggcg tagcgcccag
    31621 cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    31681 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc
    31741 aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc
    31801 ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg
    31861 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    31921 tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    31981 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    32041 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    32101 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    32161 cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg
    32221 cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc
    32281 acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    32341 tccaaacgga ggaagtgact ctgtcaccat taccgcagcg acgtttttta gctgcatatt
    32401 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    32461 aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc
    32521 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    32581 agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    32641 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    32701 ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg
    32761 gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta
    32821 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    32881 tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt
    32941 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    33001 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    33061 gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    33121 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    33181 gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc
    33241 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    33301 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    33361 cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat
    33421 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    33481 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    33541 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca ccagaggatt gcgtttcggt
    33601 gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag
    33661 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    33721 accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    33781 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgaacca
    33841 tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttgttg
    33901 gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    33961 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    34021 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    34081 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    34141 ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg acaaagtgca
    34201 tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg cctacaagtg ctcgcaaaat
    34261 cgataaattg cgattgcccc catagacccg ataaacttgc gcatcgggca acttatactt
    34321 gtcatgtatc ctttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    34381 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    34441 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    34501 gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct taaacgaaaa
    34561 atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt cagacgccct
    34621 actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    34681 gcaatagtga tttccgctcc ttccggcaca gcaattggcc ccgcctcgcc tatcacctga
    34741 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    34801 ccttcgctgc cgtcagcggt ctgcacggcc agccagccat cctgctgcaa cgcgacgtcc
    34861 agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat ctgcgcccgg cgttgacgcc
    34921 gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg gcaccgcgcg taaagcattc
    34981 aactgcgcgc gaaaaccggg cgttgaggca ttggccagat tactggcggt taccgcctgt
    35041 tgattcagtg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc cattgagcta
    35101 tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct gagttttgat
    35161 ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca gttctttact
    35221 gagatcgaca ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt tgcccgtccc
    35281 ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt tgtcgccttc
    35341 ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca atctgcccca gcagttgtgt
    35401 ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat aactcaccag
    35461 atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc cggtattttg
    35521 ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt taattgcgcc
    35581 agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa acaccagctt
    35641 cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga cctgccagtt
    35701 attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct gactgtcgaa
    35761 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    35821 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg tggtagtttt
    35881 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    35941 cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat tcaccaggtt
    36001 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    36061 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    36121 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    36181 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    36241 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    36301 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    36361 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    36421 gagcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    36481 actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat cagtcaacgt
    36541 accgtcccag gtgaaactgt ggactccggc ggtcagttca ccaatatcaa tggtgcgcac
    36601 aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    36661 aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc ccgcaagaac
    36721 agtggtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    36781 gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa tcccgctgac
    36841 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    36901 ctggtttttc agctgcgcca ccaacaaagt cagaaaactg ctttgtaagt ctgcggcgtt
    36961 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    37021 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    37081 atgcttttca ccgtgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg
    37141 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatcc
    37201 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
    37261 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    37321 tttgcccgat atggctgtcc atcgggaccg gtcacgctat cagcattcgc cagattactg
    37381 gccgccacgt tcaagcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    37441 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    37501 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    37561 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    37621 agttctgcgg taggaggcgt cagcgcctgc gccggaatgt gttgcgttga ggtcatcgtc
    37681 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    37741 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    37801 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    37861 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    37921 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatgac
    37981 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctggcaata
    38041 aaacgtagcg tggcgatcat ctcgatacta ttcactccgt taagtgcggc gagtaatctc
    38101 acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg gggtaagtga tgaggttcgt
    38161 gtttctattc gtacagcgcc caatctacta ccgccgtgcg agcagccatt gctttcgatg
    38221 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    38281 cgatatttac aggttaatgt acaggccaca ggaaattatg tggttgccgc gatgcccatt
    38341 gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg gcaggatacc
    38401 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag cctgcgcgat
    38461 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    38521 ggacaacgcg tcaacgtgat cgccagcggt gacgggttta gcgccaacgc agaaggtcag
    38581 gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc gcatggtatc gggactggtg
    38641 gtcagcggcg ttgttgatgc agatgggaat attcttataa accagtaagc tgttaaagat
    38701 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    38761 accatgagta ttgatcgcac ttcgcctctg aagcccgtaa gcaccgttca accacgcgaa
    38821 accactgacg cgtcggtaac gaacacccgg gcggcaaaaa caacagcctc caccagcacc
    38881 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    38941 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    39001 ggcaaaattg ccgatgcgct gctcaacgaa gcgcagcaag acttgcagag taactgatcg
    39061 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    39121 ggtaatggat caagaacagc aacatctctc tatggggcag atcaacggca gccagttgca
    39181 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    39241 caggaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    39301 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    39361 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    39421 tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt cgatctgaat
    39481 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    39541 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    39601 cgaccgccat taaacgcagt aagcgccagg gcatggtacc caatgaccac tccggcatga
    39661 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    39721 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    39781 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagccca gcatgtttca
    39841 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    39901 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    39961 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    40021 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacccgaa agaatgccca
    40081 acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag tccatcaaac
    40141 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    40201 tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg gcgtaataca
    40261 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    40321 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    40381 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    40441 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    40501 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    40561 ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    40621 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    40681 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    40741 cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca gaaacataag
    40801 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    40861 caaatgcctg ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    40921 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    40981 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    41041 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaggcaggcg
    41101 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    41161 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc
    41221 tgttcgccgg cggtttgcag aactgtctga ttttgcacac attcaatgaa gtgccgcgca
    41281 cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg tttatgcact
    41341 acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcatcg
    41401 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaggtggtg
    41461 atctgcaaag gatcggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    41521 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    41581 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacactattg
    41641 ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt taactcaccg
    41701 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc
    41761 agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    41821 tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggaatgc
    41881 acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    41941 cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc ttgtaacatc
    42001 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc
    42061 acaacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    42121 acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct gtaaatcacc
    42181 atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc tctacctcac cactgaaaag
    42241 atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    42301 atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    42361 atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac gcaataacaa
    42421 cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt ctgaattaca
    42481 aaaacgttgt tcatatttgg tgacccgatt accaaagcca ctcaccgtgc gcagataatg
    42541 gcgtttgacc agattatcca gttgctcctg cacttcagat tcactcagat tcatgaccgg
    42601 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    42661 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt caagggcagt
    42721 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    42781 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    42841 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    42901 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    42961 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    43021 catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    43081 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    43141 ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    43201 gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca tcacgatcat
    43261 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    43321 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    43381 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    43441 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    43501 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    43561 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    43621 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    43681 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    43741 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    43801 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    43861 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    43921 cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgttgtcgt tttttctcgc
    43981 tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg agctggctgt
    44041 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctgtgtgcgg
    44101 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    44161 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    44221 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    44281 ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    44341 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    44401 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggtctgcaac
    44461 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    44521 aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    44581 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    44641 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct
    44701 tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgcaacgcgt cgtttgctgg
    44761 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    44821 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    44881 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    44941 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    45001 acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa ctcgacggca
    45061 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    45121 gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    45181 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg
    45241 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    45301 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    45361 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    45421 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    45481 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    45541 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    45601 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    45661 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    45721 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    45781 catattgttc tgacaaactt gatcagccaa attggtcgcg aagagccgaa taaggttacc
    45841 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    45901 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    45961 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    46021 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    46081 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    46141 gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    46201 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatt
    46261 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    46321 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    46381 accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccaca
    46441 gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc cgggcacgat
    46501 ttcaccccat tgatgacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    46561 ggatttaatg aaggcgtgtt caccgctgca aaactttacc cggcaaacgc aaccactaac
    46621 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    46681 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    46741 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    46801 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    46861 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    46921 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    46981 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    47041 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    47101 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    47161 gaagcattct gttctgtaaa cggcccgcag ttctatgggt tgccggtcaa cgacacattc
    47221 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    47281 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    47341 cttcctgttg tcattaggtt atttaacctg tataaataac cagtatattc aacagggggc
    47401 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    47461 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    47521 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga
    47581 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    47641 cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt tttattttac
    47701 ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca atatgctaaa
    47761 aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag aaatttatct
    47821 gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca
    47881 gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga
    47941 tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg
    48001 ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc
    48061 aggaagggac tgaagttggt caaacgctct ggttaaccac tgatgttgcc agacaattta
    48121 tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttccag gtaaacgagt
    48181 atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac
    48241 catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag
    48301 agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg
    48361 ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc
    48421 atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg
    48481 aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca
    48541 acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg
    48601 catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg
    48661 atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct
    48721 tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac
    48781 tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg
    48841 acgatgatgg cgtaactatt gaaacggttg acggtgagta tcaggcgaaa aaagcgattg
    48901 tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag cccgtacgta
    48961 aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt
    49021 ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt
    49081 tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt
    49141 ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg
    49201 gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt
    49261 ttattatcga taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc
    49321 acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg caagacaaaa
    49381 aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc
    49441 ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc actatctca
//
LOCUS       DAZOZD010000003        47251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOZD010000003 DAZOZD010000000
VERSION     DAZOZD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.003"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1355
                     /locus_tag="ACHUPB_000111"
     CDS             <1..1355
                     /locus_tag="ACHUPB_000111"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0757043.1"
                     /translation="KGKHMTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDL
                     VVPSQFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAA
                     FKRILAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIG
                     SNEATGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLF
                     MGFAKSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERR
                     DEVHRKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADL
                     MRASYDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIE
                     KVRIADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT
                     "
     gene            1503..2618
                     /locus_tag="ACHUPB_000112"
     CDS             1503..2618
                     /locus_tag="ACHUPB_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0757044.1"
                     /translation="MSELKELIAELDFEQWLDTEGIVYRRGGVSTRGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEELVLASTVELEGPVALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGGATPEQIIKAYYRAKPYTKQLELQSKVLGIKALS"
     gene            2694..3470
                     /locus_tag="ACHUPB_000113"
     CDS             2694..3470
                     /locus_tag="ACHUPB_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757045.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVKLHVWGEPSCARRSALVVFISKENPLSLKVQVGAWLDGRVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLAPYRLESNQRKMISWGSW"
     gene            3526..4008
                     /locus_tag="ACHUPB_000114"
     CDS             3526..4008
                     /locus_tag="ACHUPB_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757046.1"
                     /translation="MAVVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTLKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEVKQQ
                     "
     gene            4005..5327
                     /locus_tag="ACHUPB_000115"
     CDS             4005..5327
                     /locus_tag="ACHUPB_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFR0757047.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAML
                     VALFKNDRSEGYTQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            5324..5539
                     /locus_tag="ACHUPB_000116"
     CDS             5324..5539
                     /locus_tag="ACHUPB_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757048.1"
                     /translation="MIVLSKREKETLHEISKWPEFPQYWKPKTRANLERLGLVANISE
                     TWCSANYQLTDKGKVLLQQLVESGVLK"
     gene            5685..5975
                     /locus_tag="ACHUPB_000117"
     CDS             5685..5975
                     /locus_tag="ACHUPB_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFR0757049.1"
                     /translation="MSNLTSWDWWLATYFLAVGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            6735..6809
                     /locus_tag="ACHUPB_000118"
     tRNA            6735..6809
                     /locus_tag="ACHUPB_000118"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6767..6769,aa:Asn,seq:gtt)
     gene            6815..6901
                     /locus_tag="ACHUPB_000119"
     tRNA            6815..6901
                     /locus_tag="ACHUPB_000119"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6849..6851,aa:Tyr,seq:gta)
     gene            6906..6981
                     /locus_tag="ACHUPB_000120"
     tRNA            6906..6981
                     /locus_tag="ACHUPB_000120"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6939..6941,aa:Thr,seq:tgt)
     gene            7472..7558
                     /locus_tag="ACHUPB_000121"
     tRNA            7472..7558
                     /locus_tag="ACHUPB_000121"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7504..7506,aa:Ser,seq:tga)
     gene            7784..9697
                     /locus_tag="ACHUPB_000122"
     CDS             7784..9697
                     /locus_tag="ACHUPB_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0757050.1"
                     /translation="MSFIDYDILSASSSKELREKLAEKIAEGWQPFESPNIVEPSKGG
                     PNAYNQAIVRSGDLSQLPTPVSNEDEEPDYYYVVTIAGQSNSMSYGEGLPMPKTFDRP
                     HPRVKQLARRSNIKPGGFACKYNEIIPADHCLHDVQDMTKFKHPKANDSEYGCVGQGL
                     HIGKKLLPFIPANAGILLVPCGRGGSGFTVTGSEGQYYEESGAIESSLKWGLNTPLYK
                     DIVARTKAALSKNKKNVFLGVVWMQGEADLATGKYSQHNELFLKMVEGFRKELSQYDA
                     QCVFGSAANVPWICGDTTHWWKNKYPKQYEIVYGGYKGKTSQAIYFVPFMKDENDNNV
                     LTNSPTDDPDITSIGYYGSGHRKDNKTWTSESRESHFGSWQRRGIISDRLATALLAHS
                     AKSSAFVNGVTGEIIKQEQPTQPKNDNIVTTPTPRTVISLTGAGGDVATEQGWTVTGA
                     NIQSVENGKAIALTKAVSNETWTISKATDIAQALLENGGKLLCKFKLSGNPDPKKAKF
                     GFGIYYKTSKPVADDISFDGTGSLFLANFFLQTIGGKLNIMMHRTGNNRNVQIDSYGD
                     YDNNWHTLELQYEKGTGTVIPVLDGKAGSKFKLVKDNIALGDEAFVLTDITNNQTHDL
                     AIETLTISINNPT"
     gene            9905..11008
                     /locus_tag="ACHUPB_000123"
     CDS             9905..11008
                     /locus_tag="ACHUPB_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0757051.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAIMISQINNNMIFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELA
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRLNFS"
     gene            complement(11003..11389)
                     /locus_tag="ACHUPB_000124"
     CDS             complement(11003..11389)
                     /locus_tag="ACHUPB_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0757052.1"
                     /translation="MSDKNIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            11637..11849
                     /locus_tag="ACHUPB_000125"
     CDS             11637..11849
                     /locus_tag="ACHUPB_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757053.1"
                     /translation="MKTTISSLITLEIGHVEKLADECVADILTELPNEQIQVGMHDAT
                     AFIFLLNNKRFTLINTGSGSLAVRTC"
     gene            11948..14056
                     /locus_tag="ACHUPB_000126"
     CDS             11948..14056
                     /locus_tag="ACHUPB_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFR0757054.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSRSLG
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSALDNHKSIESES
                     HSKHKHDNNDSDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSIEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIQLALASSYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVSSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            14152..15387
                     /locus_tag="ACHUPB_000127"
     CDS             14152..15387
                     /locus_tag="ACHUPB_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0757055.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNA
                     GIVDEYAEKMVKFAGIVRDGYEEHNISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            15568..19086
                     /gene="dnaE"
                     /locus_tag="ACHUPB_000128"
     CDS             15568..19086
                     /gene="dnaE"
                     /locus_tag="ACHUPB_000128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0757056.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRDKLAKESKGCMESLERGRNYSFTALIK
                     NEQGYRDICELMTVANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITPGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDTISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTMKTVEEALEYGAKLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLVIDAVKASRPFEMNSKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNP
                     NILYFRPEEGERLEAILADVAETIKS"
     gene            19212..19643
                     /locus_tag="ACHUPB_000129"
     CDS             19212..19643
                     /locus_tag="ACHUPB_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757057.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDDARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            19761..20789
                     /locus_tag="ACHUPB_000130"
     CDS             19761..20789
                     /locus_tag="ACHUPB_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFR0757058.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            20850..21794
                     /locus_tag="ACHUPB_000131"
     CDS             20850..21794
                     /locus_tag="ACHUPB_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFR0757059.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVASIAPQPIVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            21794..22060
                     /locus_tag="ACHUPB_000132"
     CDS             21794..22060
                     /locus_tag="ACHUPB_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757060.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            22063..23136
                     /locus_tag="ACHUPB_000133"
     CDS             22063..23136
                     /locus_tag="ACHUPB_000133"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0757061.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
     gene            23229..23429
                     /locus_tag="ACHUPB_000134"
     CDS             23229..23429
                     /locus_tag="ACHUPB_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757062.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            23433..24263
                     /locus_tag="ACHUPB_000135"
     CDS             23433..24263
                     /locus_tag="ACHUPB_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0757063.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            24359..25183
                     /locus_tag="ACHUPB_000136"
     CDS             24359..25183
                     /locus_tag="ACHUPB_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757064.1"
                     /translation="MPNHITNEIRVIGGTNKQRLDFIRAITNKKGLIDFNTICRRPKS
                     IEMEENNHIRRMASAMAGETVYDHMFGELKTPEQVELAMRESGMTRKEIRKIKEQALM
                     RLENYRRYGYYSWFDWSRANWGTKWNAYNIEMPVKRIPTRIKYGHNFRHTHVRAYAKR
                     IYKKRLARHAATGGELVIRFETAWSMPKPIYQAMARKFPHLEFVIRYADEDFGNNCGF
                     ILLRNGKWEYDYIAPANNKQSSEEKIKWRKFAFELCCPGITPQEYGLNEEYEYAGY"
     gene            25231..25971
                     /locus_tag="ACHUPB_000137"
     CDS             25231..25971
                     /locus_tag="ACHUPB_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757065.1"
                     /translation="MKRKIEEPKTTLNKHGEKIQTHPAFGLVKTSRVSTTGIRLFDSE
                     LEHRDYIEIGVYEAELSLDNERTRPQRSCNRRPLVEIRLSQAQWAAMVSSFGVGEGVP
                     CTISYKTIGDSIRVHDIAEQKSIRQKFNDQIKAATNSEIDRLNQQVSELGELVKKGRA
                     GKRDLEELYRKLASAVANLPENLCFATELMQEQMDEIVSSGKAEIEAYVAGTVMRKGL
                     AALGTESCINDDPTVSITKMESGKELLP"
     gene            25992..26381
                     /locus_tag="ACHUPB_000138"
     CDS             25992..26381
                     /locus_tag="ACHUPB_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757066.1"
                     /translation="MLLIQPGFGLKIKKGHMFGERDSKRKILSIRLPFITIHWLNRES
                     TDYWYKCARAAFNDPDWFVENHHAVRQAKRKANTTYMKAYQDAWEEHRGRYQKNIEKL
                     ESENMDLRRKLGEARRDIEAYKRLVSE"
     gene            26443..26868
                     /locus_tag="ACHUPB_000139"
     CDS             26443..26868
                     /locus_tag="ACHUPB_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757067.1"
                     /translation="MAAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPNQED"
     gene            26924..27199
                     /locus_tag="ACHUPB_000140"
     CDS             26924..27199
                     /locus_tag="ACHUPB_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757068.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            27415..27750
                     /locus_tag="ACHUPB_000141"
     CDS             27415..27750
                     /locus_tag="ACHUPB_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757069.1"
                     /translation="MKTIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            27750..27962
                     /locus_tag="ACHUPB_000142"
     CDS             27750..27962
                     /locus_tag="ACHUPB_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757070.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(28542..29756)
                     /locus_tag="ACHUPB_000143"
     CDS             complement(28542..29756)
                     /locus_tag="ACHUPB_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFR0757071.1"
                     /translation="MLSAVIDSLSAVHRMLPTVFGILSAANQVCCQRLNDKGVHMSTK
                     NKKGKNNKEVEDNIDNFEEGSLELYTGELVPNSNNTVQPIALMRLGLFVPTLKGTKYS
                     KRNKPNEIDASKELVQLEVARSEGYSDIKITGPRLDMDHDFKTWVGVVRSLAEYGEPN
                     GRVELSITKFAKFCGYPSSQIRKTLRDRLTNSLLKIMRTTLSFQRTYEEKNVDGSNKI
                     SLLMVHLINSVDYNEQKDTVVFYAEPKLAELYRFDHKVLLQLKVINKLPRKETAQALY
                     TFIESLPTKPAPVSLARLRARLNLSSRNVSSQNQTIRNGLKALQDLGYLEYSEIKRGR
                     SIYIQIHSRNPKLKVAPPKPEDIEPKKPDEKAGEIDAKQNIINKITELSQNLTPENIK
                     MIEILSNSLKLL"
     gene            29958..30602
                     /locus_tag="ACHUPB_000144"
     CDS             29958..30602
                     /locus_tag="ACHUPB_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757072.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            30857..31942
                     /locus_tag="ACHUPB_000145"
     CDS             30857..31942
                     /locus_tag="ACHUPB_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFR0757073.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            32172..34088
                     /locus_tag="ACHUPB_000146"
     CDS             32172..34088
                     /locus_tag="ACHUPB_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0757074.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKENFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEIVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            34078..34821
                     /locus_tag="ACHUPB_000147"
     CDS             34078..34821
                     /locus_tag="ACHUPB_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757075.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHENEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NSPQPTLQTAIVTNSLEARLLENPIWGTW"
     gene            34831..35400
                     /locus_tag="ACHUPB_000148"
     CDS             34831..35400
                     /locus_tag="ACHUPB_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757076.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHDLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHSKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            35474..37789
                     /gene="nrdA"
                     /locus_tag="ACHUPB_000149"
     CDS             35474..37789
                     /gene="nrdA"
                     /locus_tag="ACHUPB_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0757077.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGMTTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQELFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGIRYSDTAGNKLVHETFEAIQYYLLDASCQLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEIEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            37895..39037
                     /gene="nrdB"
                     /locus_tag="ACHUPB_000150"
     CDS             37895..39037
                     /gene="nrdB"
                     /locus_tag="ACHUPB_000150"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0757078.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWAEYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            39115..39984
                     /locus_tag="ACHUPB_000151"
     CDS             39115..39984
                     /locus_tag="ACHUPB_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFR0757079.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            40152..41255
                     /locus_tag="ACHUPB_000152"
     CDS             40152..41255
                     /locus_tag="ACHUPB_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0757080.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQTDDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRFGYESIYTKMLYDLEMDGSVTETELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDDILILGYDPLPWIKVPVAV"
     gene            41257..41670
                     /locus_tag="ACHUPB_000153"
     CDS             41257..41670
                     /locus_tag="ACHUPB_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757081.1"
                     /translation="MARGMYVLCEIEGVLANTSHRKSVPDTDAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            41667..42143
                     /locus_tag="ACHUPB_000154"
     CDS             41667..42143
                     /locus_tag="ACHUPB_000154"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0757082.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            42143..42787
                     /locus_tag="ACHUPB_000155"
     CDS             42143..42787
                     /locus_tag="ACHUPB_000155"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0757083.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            42849..43268
                     /locus_tag="ACHUPB_000156"
     CDS             42849..43268
                     /locus_tag="ACHUPB_000156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757084.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            43278..43835
                     /locus_tag="ACHUPB_000157"
     CDS             43278..43835
                     /locus_tag="ACHUPB_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0757085.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            43996..44805
                     /locus_tag="ACHUPB_000158"
     CDS             43996..44805
                     /locus_tag="ACHUPB_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757086.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            44988..45581
                     /locus_tag="ACHUPB_000159"
     CDS             44988..45581
                     /locus_tag="ACHUPB_000159"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR0757087.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
     gene            45769..45999
                     /locus_tag="ACHUPB_000160"
     CDS             45769..45999
                     /locus_tag="ACHUPB_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757088.1"
                     /translation="MSYPTNVVALVDSDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSK"
     gene            46581..47174
                     /gene="rnhA"
                     /locus_tag="ACHUPB_000161"
     CDS             46581..47174
                     /gene="rnhA"
                     /locus_tag="ACHUPB_000161"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0757089.1"
                     /translation="MTTKTPARPQAKTRKKDKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILHSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADMPHKKDIGFVYNKQ"
BASE COUNT        13672 a        10194 c        11263 g        12122 t
ORIGIN      
        1 acaaaggaaa gcacatgaca gaactttgcc atacaggacg cgggctgtcc gaagagtttg
       61 atgaagattt ccagaacaga ctgacggcat atttttgtcg tgatcacgag tttcttactc
      121 gtgcgggaga tctggttgtg cctagccaat ttgccaatgc ggccaatgcc atattggtta
      181 atatggtttc gggctattac cgtatgtaca agagcgcgcc ctcttcatct gcaattctgg
      241 atatgcttaa gcgtgcgaaa cgcgataaga ctatccgtga ggaactattc gccgatgttg
      301 ttgctgcgtt taagcgcatt cttgcagaaa aattgtccga tacctcgtac atggttgacc
      361 aggtatcaac cttcgcaaaa agtgtagcgt ttgatgatgc tctgattaag gctgctgaac
      421 tgaaagagaa aggcgacttt cagggagcga tggcaatcat ggctaaggtt cagcagattg
      481 gatcgaacga agcgaccgga atctatgact actacacctc cgcaagtgag cgattgaaag
      541 cgcgtgaata tgaggcttca gaggagtatg tgccaaacag cattacaact ggactccctc
      601 tgctcgatag gttgctgtac caaaaaggct gggcgaagcg tgaaatggtg ctcttcatgg
      661 ggttcgctaa atccggtaaa tcgaccgcaa tgggtgagtt ttccataaac gcaacgcttg
      721 ctggctacaa tgttctgtat ctctcgctgg aggttcacac caccatttta tccgaccgtt
      781 ttgatgcaag attgtcggag acagaaatgt ccaagctggt ggaacggcgc gatgaggttc
      841 atcgtaagtt ggcagagttg ggagccacga aggggattgg tagtttgtgg gtggttgagc
      901 gtccgtcagg aagtatgtca ccggcagatc tggaccgtat gcttaacagc atgaaagcca
      961 acggcatggt gcctgacatg gttgttgtcg actacgcaga tttgatgcgt gccagttatg
     1021 accttcgtga tgatcgcgcc aacattcgta gtatctacac cgatttacgt gctctttatg
     1081 acaagcataa cgttgctggg atcacggcat cgcagacaaa ccgtgaaggt ggcgcgtcag
     1141 aagttgccac aatgatgcac gctgccgaca acatcgaaaa agtacgtatt gctgacctgg
     1201 taataacgat caacaaaacc gaagaagaag aagcgaaagg agaggctcgt ctctactttg
     1261 ctggttcacg taaccagcag ggagggatca gcattcgcgt taaacaaaac ctcgaacaaa
     1321 tgcgcttcat tgagcgaatc ttagacgtta cctaaaaaat aagcgtggag aacacctcca
     1381 cgcttgattc attggtgaaa caacttttct tttgccaaac cacaaaagaa aaacacatga
     1441 gcctttatgt tatatcaaca tttagattgg tcacaatatt gcctgttaaa agtggaatta
     1501 tcgtgagcga gctgaaagag ctaattgccg aattagattt tgaacaatgg ttggatactg
     1561 aaggtatcgt ttatcgacgt ggaggcgtga gtactcgcgg tcgtgaagtg aatatcaagg
     1621 agtgtccggt atgcggcagc tccaactgga aggtatattt caatctgacc agtggcgtcg
     1681 gcaaatgctt cgctggtgat catcccgaag agattcagtt caataagctg gtcttcctca
     1741 agcactacag cggtaaatca cgacgacagt tcgaggaata tgtgcagaac gctcttcttt
     1801 cccaggggtg ggcaccaaag aaagaagagc tagtgcttgc aagcacagtc gagttagagg
     1861 ggccagttgc actccctcgt cattacgagc ttcctataga tggccgtctt ccagattatc
     1921 tggttgaacg aaacatatca cctgaaatgg caaagtattt tgacctacga tactgcgtcg
     1981 aaggcaagca cgcttatgta gatccgtata cagatcaggt aaaagggcag atattcgata
     2041 tgcgaatact gataccggtt tacgatctgg atggggtaat gaagacattt cagggacgag
     2101 acattaccgg tacagcagaa cgccgctacc tctttcctat gcagcttcca gcttcaggta
     2161 aatttctcta caacggccat aatgcagtcg gcaaacagac tgtagttgtc tgtgagggtg
     2221 cgttcgatgt tatgggggtc aaacgagcta ttttcgacga agaaacatta cgtgattacg
     2281 tggagccaat aggaacgttc gggatgcatc tatctggcaa caccactcag gatgcagaag
     2341 atcagttggg cgcgttcctg acgctcaagg cgcgtggatt acgtaatgtg atcatgatgt
     2401 gggatagtga aaagcaagct atacgcaaca cgatggccgc agccaggcga ctgaccagta
     2461 tcggtcttaa tgtcaaagtt gcgtgtttgg gtgaggaagg actcgacccg ggcggtgcaa
     2521 cgccagaaca aattatcaaa gcctattatc gggcaaaacc gtataccaaa cagttggagt
     2581 tgcaaagcaa ggttttgggc attaaggctc tatcataaca atcaccttta aaataagtag
     2641 atgattactt atctttctgt gagaatactt tcatctgtta gctaggagtt ggtatgaaag
     2701 acgaaattca gaaattagcc tgcgacatca ttgataaaac tggtttagaa atcagcgaga
     2761 gcaatcgact agacatcatt gaaaaagcgg taaaaacagc aatggatcat atcgccactc
     2821 gtttggtcga gatcccgcta ccggggctac cttatctgaa ggttaagtta cacgtatggg
     2881 gtgaaccttc ttgtgcacga cgttctgcat tagttgtttt tattagcaaa gaaaacccgc
     2941 tcagtcttaa agtgcaggtt ggagcatggc ttgatggcag agtgatctac acaaataccg
     3001 ttttttgtct ttcaaacgac gaaactattg aagcggccat tcaagaagca gttctagcaa
     3061 tgcgcagcag cggtttgatg aagaataact acgaagagta cttgcgttcg ataagtggtg
     3121 aaaagacatt atctctgaaa gcagatttcg ttaccccgaa aaatctgttg gaagtcttgc
     3181 ttaataaagg ggctaatgat gccgtaaatg taatcagaga gagtgagtat gcgtctcttt
     3241 gcgacatgtg caaaagccag ttggatctgg tgcatatcgt tattgatgct gggaaggcat
     3301 gtgatggcgt aatggcggaa tttgcttgga aggtggtcag gattgctaac gaattaccga
     3361 tgatagagca agaggctaaa tcatacgcca ccaatcatgt cacagagctt cttgccccct
     3421 atcgcttaga aagcaatcag cgcaagatga ttagctgggg aagttggtaa tctctccgcg
     3481 cgtcgttttt tacgcaaata acgataggta agtacaagat tatttatggc ggtagttgtg
     3541 aaagctgatt tgtcaaaaat cccctctatt tcaggaaata atggttattc acttcgttgt
     3601 gaggaagtaa agataaacgg tgagtcggca tattgcagct actccgtttg ccagcacacc
     3661 attcttgcct tcaaagaaaa ccgtcttcct cgaacttcat tccagtcgtg cgcaaccgct
     3721 atcaaagcag gcaaatgcaa ggcgttaaaa atgatggttg aagagattcg taaaggagaa
     3781 tctctgtatt tcgaagatat gaccgcgctc attaaggagg ttgaagaacg gaataaacaa
     3841 gccagaactt taaaacgaaa acgtgacagt gtaacgatta atagcatggt taagaagagc
     3901 accacatcac aaacagcgat cactgacgtg tatgcggcgt tgcttgaaga aacaacaaaa
     3961 gaaacacatg agcaaatcga tcaacatatg gaggtaaaac aacaatgaaa aagttgatcg
     4021 cacttaagca taagctggac gaaatgaaag ctatgggaac caatgcaaaa aaagaggcat
     4081 tggccaacat ggatgacttc gagcaaagca tggtttcatt gatgctcaac cctttcatcc
     4141 gttttggggt aaagaaatac aaagtggcag agccgcttag tgagtccatc ccaagtgacg
     4201 aaaaagccat tgatgtactg aataagctgg cctctcgcga gctaacgggg aacgcagcaa
     4261 tagcagctgt tgagtctatc gtggcgtcaa tgtgcgccga tgggcaggac gtgttccgtc
     4321 gtttcctctt aaaagacccg aaagcaggtg ttgggattag cctatgcaac aaggtttttg
     4381 aaaatcccat tccgaaattc gaggtgcagc tggcgtcacc gtataaagaa aaaggcgaca
     4441 aatacccctt caagccaaat cctaaagcaa aatggccgat gattggcagt cttaagctcg
     4501 atggtttgcg agtaatttgc gaggttattg ttgacgagga agaggttaac ttcctttctc
     4561 gtactggtaa tccaatcacg tctctcgatc acctaaagcc agcaatgcta gaattaggca
     4621 aactttcagg ccacaaacac atcttcttcg atggtgaagg aactgccggt tcatttaacc
     4681 agtccgtatc tgcattgcgc aaaaagaacg tgcaggcaat tggcgctatt tatcatgttt
     4741 tcgacttctt cctaccggaa tggcgagcac aggctaaatc caaagagtat gcaaagacag
     4801 gtatgaagct gaaagagcgc ctggctatgc tggtggcgtt gtttaaaaac gatcgcagtg
     4861 aaggctacac acaagacatt cacctgcatc cgttctatat catccatagc cacgaagact
     4921 tcatcgaacg cttcatgaaa cgcctggacg ataacgaaga aggggagatg ggcaaagatc
     4981 cgaactctgt ttacgagttt aaacgtaccc gcagctggtg gaagttaaaa gacgaagatt
     5041 cagaagatgg tgaaattatc gattttgagc cgggcgaccc ggactctggt tttgccaaca
     5101 cgcttggaaa aatagttatt cgtcttgaaa acggcgtcat cgttcgtgcg agcggcatta
     5161 agcataaata tctggacgag atctggaaca acaaagagaa gtaccgtggt cgtattgtcg
     5221 aggttcattg tcacgagaaa acacctgatg gcagcttacg ccacccacga ctgaaatggc
     5281 cgcgttgctt acgcgatacc gaagatcgaa tcggagataa agaatgatcg tattaagtaa
     5341 acgggagaaa gaaacgcttc atgaaataag taagtggccg gagttccctc agtactggaa
     5401 gcctaaaacg cgagctaatt tagagcgttt agggttggtt gcaaacattt cggaaacgtg
     5461 gtgctcagcc aactaccagt taactgataa agggaaagta ttgctacagc aattagtaga
     5521 atcaggagtg ttaaaatgat tccatacatc tcattagctt ttattggtgg cttccttatc
     5581 ggcttcggca tctgtcgtga tttaattaag caggaactta aaaccaaaac actgtgcatc
     5641 ggaaagcgtg tgtatcgggt agttcatgaa acaaaggtta gaaaatgagc aatttaactt
     5701 cttgggactg gtggttggcc acctatttct tagcggtcgg agtcgcattc gccttttacg
     5761 taggtcagtt agtcgtaaaa ctgttgctga ttaaatttgc tagtcataaa cgtatcgatg
     5821 atggtctgtg gcgtcttggc accctggtgg aaactcgcta cgggcaactt aaggagaacg
     5881 aaaccattac tatccaagcg aaacgattca ctgccaccat cacaagaaca cctagtcgta
     5941 gagtggcctt gatcaaaaaa gtcacaaccg aataaaaaca tatcgataag tatttactta
     6001 cttatccttt gtgtataaga tgactttgtt ttcgttgaga tgcgactgtt tgaacttaaa
     6061 tacaagtgca aacgaagaag tctatctggc agtagcctaa taagccaaac accagcgagg
     6121 tcagtttcca gcctcgttac cgaaatggga cacactgagc gagtgtgatt gcagaacgca
     6181 ggagggaaca ctcatgttcc ctccgatgaa gtaacagaat gggcggtcgg tatattttca
     6241 actccatatg actcccggat tcttagccac tgaccgccca ttctgttacg tcattttgtt
     6301 caattatgtc gtttatactg ggttaaaaag cggcgacgta gcccggctgg tatggttagc
     6361 cagcacacaa cgttgaggcc attacatttt tatcaattct aaggttctat tcacagagat
     6421 accggcgagc gttgatatgt aacatgttgg gcaaacattc aatcggagta gtggcctcaa
     6481 cgttgtgaag acaggattgt tgtgtaggtt taaccacttt tccacaacaa atgattccat
     6541 acatcacatt gtataaatta caaagtaggt gctgtcctca gaaacatcat ctacttaaag
     6601 attttgcctt ctattgagag aagtcgaaag cgtctgacac taacgaaaag tgcaagtagc
     6661 ggtgcgtttc ctggcagaaa ctaacaccgc cgcgattggc actgttgagt aataaatact
     6721 ggcagtgccg aattgatggt gtagctcagc ggtagagcag ttggctgtta accaattggt
     6781 cggtggttcg aatccaccca ccatcgccaa tttaggggag ttagtccgta gaggtagcgg
     6841 ggtagactgt aaatctactg tcattgcgac tcgggtggtt cgactctatc actccccacc
     6901 aaattgccga tttagctcag ttggtagagc agtcgctttg taagcgaatg gtcatcggtt
     6961 cgatcccgtt aatcggcacc aacataatat tgagaacatt ggcgtaacgg ggtcatatcc
     7021 caatctatga ataaatgttg cgttgccgcg tgacaaccag tgttctcaac attgtggtga
     7081 atgcacaggc tgatgtgccg caactacagt agtgcgcgct ttgcggggct tgctacaacc
     7141 ctgtgtcgga gatcagcgcc gaccatcaca atttggctct cttgcaacag cataacgctg
     7201 aaatatgtcc aacctggtgc ggcctgttca cccgccgtta gctccacgaa acggagcgca
     7261 caacaggtaa gaacattttc agagttcgac tctctgagtt gccattcgtg gtggcacgcg
     7321 agacgtgaat gctctttccg ttgtggttga tgcgcagact gatgcgcgga aagaaatgcc
     7381 ccagcgacac acgaggcgag ttcacacact gcaatgcagt cagtagccgg ggtcgctaag
     7441 ccggagatca gtaccggtaa ccacaacgac tggagagtag ggagcatggt gctcaagcgg
     7501 tcttgaaaac cgtcccattg cgcaagcgat gatggttcga ttccattact ctccgccaga
     7561 cacagcgttg agcggtttgg ccttttaatc aactcgatta agactccgct aacataaacc
     7621 agaccgctca acgctgtgtg tgaaattcca catattttac acatatccat ttacgcaatt
     7681 tttattgttc tttcatacat tactcttata ttttattgtc gttttttaac tgctaaaatc
     7741 gatacgattt tagctaaagt ttatcgttaa aggagtaacg attatgtcat tcattgacta
     7801 tgatattttg tcagcatcat caagcaaaga gcttagagaa aaacttgcgg agaaaatcgc
     7861 agagggttgg caacctttcg agtcaccaaa cattgttgaa ccaagtaagg gcggaccaaa
     7921 tgcgtacaat caagcaattg tgcgatctgg tgatttatca caattgccca caccggtatc
     7981 aaatgaagat gaagagcctg actattatta tgtagttact atcgcaggtc aatccaactc
     8041 aatgtcatac ggcgaaggac tgcctatgcc aaaaactttt gatcggccac atcctcgcgt
     8101 aaaacagtta gctcgacgca gcaatattaa accaggtggt tttgcctgta agtacaatga
     8161 aataattccc gctgatcact gtcttcatga tgtacaagac atgactaaat tcaagcatcc
     8221 aaaagcaaac gactctgaat atggatgtgt tggtcaggga ctacacattg gcaaaaaact
     8281 attaccattc attccagcaa acgcaggtat tttgctcgtt ccttgcggaa gaggtggttc
     8341 tggtttcact gttacaggct ctgaaggcca atattatgaa gagtcaggtg caatcgaatc
     8401 atctttaaaa tggggattaa atactccttt gtataaggat attgtagcca gaacaaaagc
     8461 ggccttaagt aaaaacaaaa agaatgtatt tcttggtgtt gtctggatgc aaggcgaagc
     8521 tgatttggca actggcaagt actcgcagca taacgaactg ttcctcaaga tggtcgaagg
     8581 tttcagaaaa gagctatctc aatacgacgc acaatgtgtt ttcggaagtg ctgcaaatgt
     8641 accctggatt tgtggcgata cgacccactg gtggaaaaac aaatatccaa aacagtacga
     8701 aattgtttat ggtggttata agggaaaaac ctcacaagca atttatttcg ttccattcat
     8761 gaaagatgag aatgataata acgtattaac caatagccct acagatgatc cagacatcac
     8821 atctattggc tattatggtt ccggtcatcg caaggataat aaaacatgga cttccgagag
     8881 tagagagagt cattttggtt catggcagcg acgcggaata atatctgatc gtctcgcaac
     8941 tgcgctgctt gcacattcag cgaaatcatc tgcttttgtg aatggcgtta caggtgagat
     9001 tattaaacaa gaacaaccta ctcaacctaa aaatgacaac attgtcacaa ctccaacccc
     9061 gcgaacggtt atttccttaa ctggagctgg tggtgatgtc gctactgaac aaggatggac
     9121 tgtaactggt gcaaatattc agtcagttga aaacggaaaa gcaattgctt taactaaagc
     9181 ggtttccaac gaaacttgga cgatcagtaa agctactgac atagcacagg ctcttcttga
     9241 gaatggaggt aaattacttt gtaagtttaa attatctgga aaccctgatc caaaaaaagc
     9301 taagtttggc tttggtatat attacaaaac cagtaaacct gttgctgacg atatatcatt
     9361 cgacggtact gggagcctgt ttctggctaa cttcttcctt caaacaattg gaggcaagtt
     9421 gaacattatg atgcacagga caggcaataa tcgaaatgtt cagatagact catatggtga
     9481 ctacgacaac aattggcaca ctcttgagtt gcaatatgag aaaggcacag gaactgtaat
     9541 tcccgttctc gatggcaagg cggggagtaa attcaagctg gttaaagaca atattgctct
     9601 tggtgatgag gcttttgttt taacagatat aacaaacaac caaactcatg atcttgctat
     9661 cgaaacactt accattagca tcaacaaccc aacctaacac aaagtaacag ccaggcatga
     9721 aattgcctgg cttcatatcg aatcaaatat ttaaaactag ctctacagaa aaaaatgatt
     9781 ccatactctt taggtatgga atcattttcc gcatgaaaga ttttagcttg attccaaaag
     9841 aatcaatgcc taatatactg gttacttata caggtgatca gcgtaactag gtgaatgcca
     9901 ataaatgaaa aatagctttg acagagcacg cgcagcagaa aacacctcaa aagaggcaat
     9961 tgagtatctc gaaagagcat ctcaaatgca ggccattatg atctcacaga tcaacaataa
    10021 catgatattc tcggacgcat tcatgttatt cactcgctta tctctgctga taaccagacg
    10081 tcggccagag atcgctgttc attgtatttt gatacatgtt ttgccgcaca ttgctgatgt
    10141 aaaagtaagt gacattaata ggttcatggt taaccaactg gtcaaccctc taatattgga
    10201 tggcaaaatt gttatgggcc gccgcgtttt ctctctgatg aagcagttcc ttagctggtg
    10261 cgccttccag gggatgatag acgtgtcacc gttaaacgat atgtcactaa acaaagttgc
    10321 cggtggcgca aagcccacac ctcgcgagcg gaagctgacc gacgcagagg tatgggtgtt
    10381 ctggaatata tgggactact tcaatgtgtg cgctggtaca aaatgggcgg ccaggctatg
    10441 tcttgtatca gcaagacgac ctgacgaagt actgagggct aaaaaaagtg agttcaatct
    10501 taagcgtgga gtttggaatc aaggcaagag aaacaagtca gcacgtgagc attcgctacc
    10561 tttaagcaca ttaatgcgca catgcattga agagttgttc gaatacggta aagacagcca
    10621 gtggctcgtg ccttcgaata aaaaaatcgg gaaagacctt cctatgtcta aagtggcaat
    10681 agcccaggca ttacgtcgta ttctggaacg accagaactg atggagcttg cgccatttac
    10741 accccgagat ttgcgccgta ctgcgcgtag ttacttccca gcattaggca taagccagga
    10801 ggtatcacgc aaaataatga accacagtct tgaggggata gatcgggtct acgaccggca
    10861 cgattatatg gacgagatgc gagacgcctt agaaagtttc tcaacgtaca tcgcatcaat
    10921 cgtagagcaa ccggatttag acgaaattga ccacaaattc aagggagatc gtctatcaac
    10981 agagcttatt cgtctaaatt tttcatagag actttatggc ctcaacaacc ttttgtgatg
    11041 cgcctttctc tttaccgaat cgctcgttat atgcagcaag aacctgtttt tcgtcctcgt
    11101 taagaggagc ggtaccttct ttgtataaaa atgctgcgag ttcaggttgg cgttcttcca
    11161 gcaccatcat cataagacga cttggctcaa tacccagcgc caacgccagc ggacgaacct
    11221 tatcgatagg caaaggaatt ttaccacttt taattaaaga aaggttgttg gcatttttat
    11281 aaccaataag tctggctatt tgggcctgac tcataggtga ggattcaatc agccctgcga
    11341 taaaagcagc atagcgactt tctataaatt caatgttttt gtcagacatt attgcgacct
    11401 ttgcgcgtta aactctctgg taagtgctta ccgatattac atcaaaggtt agggttgtaa
    11461 agatataatc attttttcta caggcacaaa tattaaccaa tcagcattca ttaatggaga
    11521 aaaacgacta ataacatgta acaaaactaa ctcagatatg atatagataa gttcaattat
    11581 gatataagat ttaatagtac tccctaccat attttaagtt agaatggatt gattgaatga
    11641 aaacaactat ttccagccta atcactcttg agatcggaca cgttgaaaaa ttagctgatg
    11701 aatgtgtagc tgacattctc accgaattac caaatgagca gattcaggtt ggtatgcatg
    11761 acgcaacagc ctttatattc ctacttaata acaaacgctt cacgcttatc aataccggct
    11821 ctggctcttt agccgtcaga acctgttaac ccctctgcta cctgcgcgaa tggcttagtt
    11881 ccctgttcgc gcagtgctac attaaacaca ctagtaaata atttgttttc ataacaaagg
    11941 attagccatg tctaaaaaac gttccatcaa agaggttcag gacttccgtg acagtgtaaa
    12001 acgagtagtc gctctccttt caggtaaaaa catccctgtt gcagaacgag gagacgacgc
    12061 ttatgtacgc tataacgatg atggagagcc aattctcgta aacatcccat caattccgga
    12121 taacgcaaca ccggcattga tgaatgctgt gcgcggattt ctcgatcatg aggttgctca
    12181 cattttgttt accgatattc gtgtctccaa caaaatgaga gaaaaaggac gcgttccttc
    12241 ccggtcacta gggaatgcat tagaagacgt gttcatcgag cgaaaaatgg ggcaggtctt
    12301 taacggaacg agacgtaatc tgatggcaac tcagcgcctt ataatcgaaa aagtctttaa
    12361 accaaaggct tcagaggcta ttgcttattg tggcaaagat cagcgcgcgc tttttctaaa
    12421 cttctttctc tgtccggttg taagagcctg ggatggccaa gcaccgttcg tagatttcat
    12481 ggatgaatac tggcctgtca ttgagaaacc aatttcatta ttaaaagaac atggtatcga
    12541 tgtggccgtg cgtaacatgt cttgcaccga ggattgtgta aaggtggctg cgaccatagc
    12601 taagatcctc aaagacactg aaagtgaaag caaaggtaag gagtcagctc cgggaaaaac
    12661 ttccgatcct tcagacgctg accagacgga tgcctctgga gaaaacaatg aagacaacga
    12721 agatcatgag acaccctcag cgttagataa tcacaaatct atcgaatcag aatcacacag
    12781 taagcacaaa catgataata acgacagtga tgattcagat aattctgaat catcagaaac
    12841 aatattcgat gatacagaaa atgataaaga ggtatcagat tctgatgctt ctgataacgc
    12901 ggcgtcagaa tcattaaccg ctgaccacga aaaaagaaaa acgacagaag acggctcttc
    12961 agatattcca actccgtcaa aaatgagtct ggaagaggct ttagaggagc tggatagcat
    13021 agaagatgaa gtcggaggca tgacagaaga tgctctatcc gaaacgatta aaagcgagtt
    13081 aacagaaagc tcgaaaagcg aatacaggcc atacaatcgc tcatacgact tcatcggctc
    13141 gattgatcag gcagaagccc atatcaaacg gcttattaaa acattctccg atattgattt
    13201 aggaggatat ccaatcagcc gctatcgcat cgttcctgaa ggcaaccagc tcttcgacaa
    13261 atatattgaa aagcatcttt cgtcaggtgt ttcgtcgacg ctggcaaaag acctggagcg
    13321 agcaatagca agcagaaaca gagttcagtt tataccgggc cagcgtcggg ggcgcattca
    13381 tggttctagt atctacagat tagcaatgaa tgatgatcgc gtgtttcgta aaaaagaaga
    13441 atctaaagcc gttaacgcct gtgttcaaca agtgattgat ttatcaggtt caatgagtgg
    13501 tataacgata caattggctc ttgcaagttc atataccatc gccgatgccc ttgatcgaat
    13561 aaatgttccc aacattatca ccggcttcac tacatttggt agtcatatgg cggcaggaga
    13621 acttaaggct gtcaagtatg agttctctcg ctttgaatct ttaatgctac ctatcatcaa
    13681 aaattggaat gaaaaggtaa gttctcgcga agttcgctca cgtatggggt gcgtaggcta
    13741 cacattccca cttcttaata acgtggatgg tgaaagcata gccagccttg catcgttatt
    13801 ttccggtcgc atggaggaca ggaagatcat gcttgttctg agtgatggcg cgccgtgggc
    13861 tgttgggaga ggttttgacg ctcatttgcg ttcggttgcg aagcaaattg aaacgcagac
    13921 tgacattgat ttgatggcaa ttggcatcat gactgacgca ccggagagat tttactcaaa
    13981 tcatgccctg gtaacgagcg ttgatagtct tggttcatct gtagttactg aactatctcg
    14041 tatcattttg aagtgattag aatagcctta atgataagta atcacttacg atatttgatg
    14101 gtatatttat ataagaagtt gaaagctcat tagaaaacaa aggaaaaacg catgactact
    14161 actgcactgc aaaatgaaaa aaatccttct gattaccttg tttgcaagtg gtgcggaaaa
    14221 tcatttcact attttaagtc ccatgtagcc aatggtaatt gcgagggcat tcctgagtca
    14281 gtaaaagatg ccgatcctga caccgtactg aaaatgtaca caacacagtt tccagatgag
    14341 ccaacgctat cgaaaaaggc acttgatgca attcaagcta aacgtgccga gcaaaaaagc
    14401 gaaatggcca aatcttctgg cttgaccagt agccctggct acacaggcac agttgagtac
    14461 aagacagatc tggtcgcagc tcacgaactg ctaaacgtaa cggtggaaga actcggaaca
    14521 aaacgtggga cgccgctcat ggttagcgtc aacgtcaata cgccgtatcc agagttcgtt
    14581 cccgaagtga agaaaggcta cgtatatggc gacttcgaac tgatcaaaga tattttcatg
    14641 atgcttgaac ttggcatacc tggctatttg tggggtcatg caggaacagg caaatcgtca
    14701 ttgcctacac agctatgtgc tttgctcaat cgtccgttga tccgtgccca acatacagca
    14761 tcaatggaag aggcacatgt tacggggcaa attctggcgc gtgatggctc tacgtatttc
    14821 gaacctggct tgcttgcgct cgcaatgaag catggctggg tttacctcgc ggatgaatac
    14881 gactttgcgt ttccacaaat tcttggcgtg tatcagccag tgctggaagg tgaagcgttg
    14941 gtcatcaaag aggcaactcc agaatggcgt cgcattactc cgcatgaacg gtttgctttc
    15001 attggcactg gcaacacgaa cggatctggt gatgaaaccg gcttgtacca gggtacaaac
    15061 atccagaacg ccgcgaactt ttcgcgtttt ggcatcgttt cgaatgtgaa atacatgagc
    15121 aaagacgcag agatcaacat gttgacaaat gcaggcatcg tggatgaata cgctgaaaag
    15181 atggttaagt ttgccggtat cgttcgcgat ggatacgaag aacacaatat cagccaaccg
    15241 attgggcctc gtgagctttt gttgtcggcc aagattggaa tgatgcgagg cgattttgtg
    15301 acaggtattg agcgttcttt cattaacaaa ctcccttcag cttctgcaca agcggctcgt
    15361 gaagttgttc aaaaaatatt tggttgatcg tgcgtaaagg atgtttcggc tctcttatcg
    15421 ctgcttctga aactggtaag gcttgtctgg agtgtccaga caagccttat tgtcaccaat
    15481 cagcaaaaga agttgcgatt tcgatgcatg ggaagttcgt aggcttccca aatgacaaaa
    15541 tcaaaaaaac cagaaaggta aaaacacatg aaggcactga tggttcgaac tgacttctca
    15601 cttggggagt cggctctaaa agcagaaaac gcggtgaaga ttgccagaga agctggctac
    15661 accgctgtaa tttcagcaga tagcatgaat attgcgagtg ttattccact acaacgtgcc
    15721 gctggtgacg acatggcggt tatttgtggt gtgaaactaa acatcgttga tgatcccaca
    15781 tacgagcacc gggataaact tgctaaagaa tctaagggat gtatggaatc attagagcga
    15841 ggacgtaact actcgtttac cgctctaatt aaaaatgagc aaggatatcg cgacatctgc
    15901 gaactaatga cggtggccaa cacacgagaa cagttctact ttgtaccgcg tctctcgctc
    15961 gaacagttgg tttctacata tgccaaaggc aacatcatcc tgctcacttc cgacatcggt
    16021 agcgtgttcc aacgcaacga ttttgcaaaa atcataagca cactgattac accgggtgga
    16081 aaagacaact tctatagtgt ggtttatccg caccctaccc cattctacga ccagattaac
    16141 gtccgggcga tgaaagtcgc cagcgcattg aaaatagagc cagtggcgtt ctatcccgct
    16201 tattacgaat cgatcgacga tgcagacatt aaagacattg cgcacatggt tacgaacaac
    16261 ataaaaatcg accagccgca tcgtctgcgt atcccccacc agcgagataa cgccgtcaat
    16321 ggtcgccgcc atctccttga ggcgcttaaa gccttctccg ttcgcatgga tgtgccggta
    16381 acagctgcaa tggcctcaac aacgcaggat accattatcg atgcctgcac atggcgctgg
    16441 catgaattgc caccagcact gcccaagatg gcagacgacg aacctgcaac gctgatgaaa
    16501 ctggctgttg cagggctgcg taaacgtctt accacaaaag agtttggata cacaccacct
    16561 gcttctgaga acagggttta tgttgagcgg ctaaagtacg aaatggacac gctgactcgc
    16621 ctgggattct gtggttactt cctgatggtg cgcgatctga tgaatcatag tcgtgaaact
    16681 ggcattcctg tcgggcctgg tcgtggttcc tccgctggat ctctggtggc atggtgcata
    16741 ggcataacca acgtcgaccc aatccgtcac ggtcttctgt ttgaacgttt catcaaccct
    16801 gagcgtctcg acttgccgga tgcggatttg gacttcagcc aggcacgtcg ccatgaagtg
    16861 atcgagtatc tgaatgaacg ctacggcgaa gattacgttg caggcattcc gaacttcacc
    16921 tacctgggcg cagcctctgc actacgtgat accgctcgta tttatggtgt ggagtccgca
    16981 gatatggcgg tatcaaaaga actgaagaac gtcgaggatg atagccttcc attggaagag
    17041 ctgcgcgaac aactggcaag tctcgacaaa tacgcaacaa aatatcctga tgcattcaat
    17101 gcagcctgca agttacaaag ccttatgcgt ggctttggta gacatgcggc agggatgatc
    17161 gtagcaggtg ttcctctgac agaacgtaca ccggttgagc gccgtggtga cgcgcgttgt
    17221 atcgcatttg acaagcgtta ctgcgaggct atgggcctaa ttaagctgga cgtgcttggc
    17281 ctggcaactc tcgatttgct cgatagtgca aaacgctaca ttaaagagaa cacaggtgaa
    17341 gatatcaatc ttgataccat ttctcttgaa gatcgcaagg tgctggatgg ttttgctgct
    17401 gggtacactc aaggtgtttt ccagcttgaa tcaggcccaa tgcgcaagct gcttaaagat
    17461 ctgggcggtg gcattgagcc aatgagcttt aaaaccgttg tcgccacgac tgcgctcttc
    17521 cggccgggac caattcagtc aggcatgttg gatgattatg tttctgtcgc caaaggcttt
    17581 atgacgccgg aatcattaca ccccgttctt gatgaactta ccgcagaaac aaatggcgtg
    17641 attctctatc aggaacagac gatgaacgcg actcgattgc ttgctggctt cacaatggct
    17701 gaagctgacg gcgttcgtaa agcgataggc aaaaaagaca tggaaaaaat gaagagcatg
    17761 ggcgagaagt tcatcgttca ggctcaagct ggctggatag acgttgagct ggaagatggc
    17821 actacacagc gcattcaccg tgcggaacat tttaaatgcg aagacggaac tatgaaaact
    17881 gtcgaagagg cacttgagta cggcgcaaaa ctacctataa acgcagtacg cgttacagcg
    17941 tcacatccag ggctatcaga gatgaaagcg aaggagatct ggaccgcatt cgaaaagaat
    18001 ggtgcctacc agttcaacaa atcacactct gttgcttact ccttaatcag ttatcaatct
    18061 atgtggttga aaactcatta tcccgcagag tttttcgctg ctgctctcac tattcttggc
    18121 gaagataaac accaggggct ggttaaagat gcgctgacct atggtattcg cgtattgcca
    18181 ccagacgtta acgtgtcatc taaccgaatt gagatccgca cgcttgaaga tggcagccag
    18241 gtgctgtatg cgcccttctc tgctgtgaaa ggttgttctg agaatggctg ccaggccatc
    18301 atgagagcgc gagaaaaagt tggcggcaaa ttcgagtcac tggcgcaatt cgaagaagct
    18361 gtcgagaaac gtgcctgtaa tagtcgagtg cgcgaatcac tgcaaaaagt aggggcgttt
    18421 gcatccatcg agccaggcag tctgccagca actgatccag aacgtctccg cgaccaagcg
    18481 gaattgatgg gaaatcttgt catagacgca gttaaagcct cacgtccgtt tgagatgaac
    18541 tccaaacgtt cggctgaaat taacgtactt atgacgcgga tggcggctga aatgggctta
    18601 ggtgatgaac taatccgccc cagcattggt attaagccga aaatcatgat cattctggac
    18661 aatgcaaacg gcaatgatgc tcgaaccggt tacttcatgg agaacggata cgacgatttt
    18721 aaggccaagc tgttgacggc tggagatttg cgcatgggcg atctctatgt cacaggcgtt
    18781 tgcaaaaagg tgaaagacaa agaaaaggac tacaccaaag acgagatcgg ccagttcacc
    18841 gactttatgc gtgaagagat caatctggta cgtccgacct atgtgctgac gtgtggcagt
    18901 cgggcaacgg cattgtttaa caacaaaaac aagccatccg atttagtcgg taggaaggag
    18961 tatcttccag accttgacgt tacagtcttt tacgggttta accccaacat tctttacttc
    19021 agacctgaag agggagaacg actagaagcg atattagccg acgtagcaga aactattaag
    19081 tcgtgatcca ataaacatgg cctctatggc catgttttct cttatcccct catatcccct
    19141 atcctcgaac tccttgcctt atcatcacaa tgatataatc aatataagat gataagtaaa
    19201 aaggaaagca catgatcacc gatatttacg agaaaataat gtctgatctc gagtttgacc
    19261 gagacaatct ggaggaagtc tggcgtagac aaccccgcct tttaatggag tatggctcaa
    19321 aactcgctca tgcagaaaga agtgtcgcag aggcaaaact taaccttgaa gctgttgaag
    19381 caaagctata cgacacagag cgtaagaact tgagtatgaa cggcattaag ttcaacgagt
    19441 ctgtactgga cgctaaggtt aaaacgaacc cacagtatct atctaaacgt cagaagttgg
    19501 atgacgcacg gcacatagca gacatataca aacatgctgt cgccgccttt tcgcatcgcc
    19561 gagacatgat cgttcaggcg tcaaagatgg ccatcgttga attagagcga ttaggctctg
    19621 aacgctttat tactccccgt tgatttttga tagataataa gtaagtactg atctatcatt
    19681 taacagctcg aaagagccac gaatgaacga aagcccaacg cgcatagcgc catcggccaa
    19741 atcacaacaa ggagaaacac atgtctaaga cattacttga tttgcttaac aaaactcgtg
    19801 aagacattgc cgccaaacgt ggcaacaacg ttgatctgac tcgcttaaaa gacggcgtca
    19861 actatatccg catcttcccg aataaagacg acccaaacgg taagttcttc cagactttcg
    19921 gtatgcacta cgttaagtat cagaacgagg aaggtaaaga agcaaccaac gcttatattt
    19981 gtgagcaaca tactcacggt cgcgcttgtc agctatgcga aatggtgatg gaaggtcgcg
    20041 ctcgtcacaa gggtaacaaa gcaatggaag aacgcatcgg tcaaatgcgt gccactcctc
    20101 gctacctggt caacggcatt ctttctgctc gtgaggattt cgcagatgct gagaaatgcc
    20161 aattaatcga gctgccgtct actgtattcg atgatatctg caaagcaatc accgaagaca
    20221 tcgctgatga tatcggcaat ccactgagca aagaggaagg ctacgcattc ctgattaaac
    20281 gtactggctc tggtcgcgat accaaatatg acgtctcgcc taagcgtaaa gtctacaaag
    20341 gcgatatcga agataaattc tggaacaccc agcatgatct gatcgcatac gcaaatcagg
    20401 ctgatgaaac tcgtcttctg tcgacagttc gcactatggg tcgtctgatt ggcatcgctg
    20461 caccaactgc cgcagcatct gcaccagcaa tttcctcaac cgcgaaaaca tcggctgcgg
    20521 cactacctgg atttggctct gtcactggtc atacagaagg agcgacggct gtagcaaccg
    20581 cgcacacacc ggcttctgaa ccaaccagtc tggttgatga agaaatcctc cgtgccgttg
    20641 aaactgaatt taaaccagag gcaagttccg ctgccgttgc cgtatcagtc aaagagtctg
    20701 aagcagtcgc agcgacatct gtagcagccg catctgcgac ggaagatgaa ggtctggatg
    20761 acctactgag agagctggac tctctgtaat cccattacgt gaccagtaag gcgtctacgg
    20821 acgccttact ttttggaagg aatgtaccgg tgaattatct cttcgtagat ggcaatagcc
    20881 tgggttatta ccaccaacaa tctgacaaat tgcacaacgg cgaaatggaa gtacaggctg
    20941 ctttcggctt tgttaagaac gtccgtcgtt atgcctccat cctccatgcc cgacctatga
    21001 ttctttggga tggatttagt gacaagcgtc gcgactttta cccggactac aaagcaaatc
    21061 gcgacgacga tcctgatatg aaaaagatga aggaaggctt tgctatccag aagccataca
    21121 tcctcaaaat gatgaccgcg cttggagtta cccaactcat tgcaaaagat gcagaagcgg
    21181 atgatctggc cgggctgctg gtagccagca ttgcaccgca gccaattgtt gaacacatct
    21241 atctgttaac aggcgatagc gactggcttc agttagttcg cgaaaacgta agctgggtaa
    21301 gcctgcgcga agacgccaaa aacaagcagg ttaattttga gcaatttgcg gagctgacag
    21361 gattcgctac tcctcgcgca tttttggaag caaaagcatt acaaggcgat aaatcggaca
    21421 acattagcgg tgttggtggc attggtgctg gcggtgcgaa agagctgctg catgaatggg
    21481 gaagtgtcgc aacgatggta cgcggcatca acgacggctc aatcgtggtt gacaaaggac
    21541 gccataagac cgccttcaac aaactagcga agaatgcctt caacgagaaa acaggctgtc
    21601 gaatgctcga agcgttcaag agaaacatca cgctaatgaa cctgattgag acgaagtttc
    21661 cgcctaccga aatcgaaaca atcaaaggca atcgtgacgt gaaagcattc gagcaactgt
    21721 gctacgagct gaatttccgt tcgttccttg aagaccttga agtgtttgtt cttccattcg
    21781 aaaggtattg ctaatgctta aatcgattat taatggcgct acaaccaccc ctacccaact
    21841 ggcaaaagag attgtctttt atcacggtga gtacgctgtc atcgcactgc cgtcaattct
    21901 aggcgctgcc ggaatgaaag cgacagatcg cgagtttgga ttagtcagcg agcaggtcgt
    21961 aaaaatcctc gctcgtgtat ccagactcct taaccacgat gcgattgtat tcgacgaatc
    22021 cgccgcttta aaacgaatca acgaaacaaa aggagcctga tcatggcaaa aggaaaatcc
    22081 gcactggcac ttgctctgaa aaagaaaatc ggtagcaacg acgaaattca gaaagtaact
    22141 cattggattg acacaggctt tcctccgtta aacaaagcga tttccggtcg ttacgatggc
    22201 ggtttcccat gtggtcgtat cgtagaagta ttcgggccac caagcgcggg gaaaaccttt
    22261 ttggcaacgg cagcaatgat ctccgcacag aaacaagatg gtctggcggt attcctcgat
    22321 catgagaaca gttttgacgt tggcctggct gtagccaatg gtttgaatgc agatgaggat
    22381 gacggtcagt gggtatacaa gcagcctgat accttcgaag actctgtaga gttgatcggc
    22441 acaattctta aattggtacg tgatgaagag cttattcctg aatcagcacc tatctgtatc
    22501 gtggctgact cacttgcgtc tatggttccg aactccaaag ccgagaagtt cgaaaagatg
    22561 gctgaaggca ctgccaaaga caaagatcag ctaaacatga acgacaatac ggcactggct
    22621 cgtgcgacaa gtgcgaactt ccctactctg gctttgtggg cacgcaaata caacgcctgc
    22681 atcatcttct tgaatcaggt tcgcacaaaa atcggtgtaa tgtttggcga ccctactacg
    22741 tctccaggtg gagattcacc gaagttctac gcttctgtac gtatccgtct tggtgcatcg
    22801 gtgatgaagg atggtaaaga gaagatcggc caggacgtag gcgcagaatg catcaaaaac
    22861 aaagttgcac caccgtatgg caaatgcacc tggaaattct acttcgatcc tactcgtggc
    22921 ctcgacgtta tcgaatcgct cgtcgagtac atgctggaag aaggatacct gccaaagaac
    22981 gccagcgggc gagttgaaat tggtgacaag aaatacacca aatcgcagat cgtcgagatg
    23041 tatcgggaga agccactggc tgaaatcatt gcggctttgc aggcaatcga cgaccgaaga
    23101 gcaaaagaca accccaccga gtcagtagaa gagtaaacac aaggcgtcca caggacgcct
    23161 tttttatctc ttgaaaatat ataagtactt acttattatt ttcgcataac aaccacatag
    23221 gaaaacacat gatcaaaatc tatctattgg cagtagccac aggcgtttca gtggctctca
    23281 tctacggttt actggttccg tcgctgattt ctaccaagag tgatttagcc gtcatgtttg
    23341 gagttatcgt tggttttggt gctcctgtaa tcggtcttat tgctggtcgt aagtttatca
    23401 actcattaat caaagcaaag gggaaataag taatgaagaa aggtttactt gcagttgctc
    23461 tggcggctat ttgcacaatg ggtcttactg gctgtgatcg cgtggagcct ggatacgttg
    23521 gcatcaaagt aaacaaatta ggtgaagaca aagggatcgg tgaagtggtt ggcgttggtc
    23581 gccaatggac aggtcttaac accgaacttt acgtattccc gaccttcaaa caaatgaaga
    23641 cctacgacga accgttcaca ttccagatga gtgacggtac tgctattggt cacaaaattg
    23701 gcgttgcgta tctggttaat cgtgacaagg taacgacggt gttccagacc tatcgcaaag
    23761 gcgtagacga tatcaccgaa tcagatctgc gtcagaaaat tgccgactct ctaaaccgtt
    23821 tggccagccg tatgaccact gactcattta tcgacggtgg taaggcgcaa ttgctggaca
    23881 acgcactgaa agatattcag aaagagatgt ctccggttgg tattgaggta ctgagcctgt
    23941 catgggttgg aaagcctgat tacccaaaaa ccgtcattga atctatcaac gccaaagtaa
    24001 cggctaacca gcgtactctg caacgtcagc aggaagttga acaacgtaaa gctgaggcga
    24061 atatgctacg tgaacaggct aatggtgaag ctgatgctat ccgtgctcgt gcgcaagcag
    24121 aagcagacgc cattcgtctg cgcggtgaag ctctgcgtca aaacccgaac gttatggaac
    24181 tggaagccat caataaatgg aatggccagt taccgcagta catgactcaa ggggctaaca
    24241 ctcctttcat tacagtgaaa taactctcat taagaagatc aggcgtccag ttggacgccg
    24301 ttttattctc gttattatca cacacaagaa aacaatttgt taaaaggtta agttaattat
    24361 gcccaaccac attacaaacg aaatccgagt cataggtggt acaaataaac aacgcttaga
    24421 tttcatacgt gccataacta acaaaaaagg tctaattgac tttaatacca tttgcagacg
    24481 tccgaaaagt atagaaatgg aagaaaacaa tcatatcagg cgcatggcct ctgcaatggc
    24541 gggcgaaaca gtttacgacc acatgttcgg tgaattaaag actccagaac aggtcgaatt
    24601 agctatgcgt gaatcaggaa tgacacgcaa ggaaattcgc aaaatcaaag agcaggctct
    24661 tatgcggctt gagaattatc gtcgctatgg ctattactca tggtttgact ggtcgcgagc
    24721 taattgggga acaaaatgga atgcctacaa catcgaaatg cccgtaaaac gtatccctac
    24781 acgtattaag tacggccata atttccgcca tacacacgtt cgagcatatg caaaacgaat
    24841 ctataaaaaa cgtcttgcaa gacatgccgc aactggtggt gagttggtga ttcgctttga
    24901 aacagcctgg tctatgccaa aaccaattta tcaagcgatg gctagaaagt tcccgcacct
    24961 tgagtttgta atacgttatg cagacgaaga cttcggcaat aactgcggat tcattttact
    25021 tcgcaacggc aaatgggaat atgactacat tgcaccggcg aataacaaac aatcgtcaga
    25081 agaaaaaatc aaatggcgca agttcgcctt tgagttatgt tgtcctggta tcactccaca
    25141 agaatacggt ctaaatgaag aatacgaata cgccggatat taaaaaaaat tgtcatttag
    25201 ctttacgcat gaagcataga ggttaataag atgaaaagaa aaattgaaga accaaaaacg
    25261 acccttaata aacatgggga aaaaattcaa acacatccag cgttcggtct tgtgaaaaca
    25321 agccgtgtta gcacaactgg cattcgccta tttgattcag aactggaaca tagagactat
    25381 attgaaatag gcgtttacga ggcggaactg tccttagaca atgaacgaac ccgcccacaa
    25441 agaagttgca atagacggcc tttagttgaa attagactta gccaggcaca atgggctgct
    25501 atggttagca gttttggagt gggcgaaggt gtgccatgca ctatttcata taaaaccatt
    25561 ggagactcaa ttagagttca tgatattgcc gagcaaaaaa gtattcggca aaaattcaac
    25621 gatcaaataa aggccgctac gaatagtgag attgatcgac ttaatcagca agtttcagag
    25681 ctaggtgaac ttgtgaaaaa aggaagagct ggaaaacgtg accttgaaga actatataga
    25741 aaactagcat ctgctgtagc taaccttcct gaaaatctgt gctttgctac cgaattaatg
    25801 caggaacaaa tggatgaaat tgtctcatct ggaaaggcgg agattgaggc gtatgttgcg
    25861 ggcacggtta tgcgtaaagg gttagcagca ttaggcacag aatcatgcat taacgatgat
    25921 cctactgtct ctatcaccaa aatggaaagc ggtaaagaat tactgccata aatgcatctg
    25981 aggtactgac aatgctttta attcaacctg ggttcggttt aaagataaaa aaagggcata
    26041 tgtttggtga gcgagattcc aaacgaaaaa ttctgtctat tcgattacca tttatcacca
    26101 ttcattggtt aaacagagag tcaaccgatt attggtataa atgcgcaaga gcagcattta
    26161 acgaccctga ctggtttgta gaaaatcacc acgcggttcg tcaggcaaaa agaaaggcta
    26221 atacgacata tatgaaggca tatcaggatg cctgggaaga acatcgtggc cgataccaaa
    26281 aaaacattga aaaacttgaa tcagaaaata tggacctaag aagaaaactc ggtgaagcac
    26341 gtcgagacat cgaagcatat aaacgtcttg tatcggagtg aatatatctt tgctttattt
    26401 tttcaaaggt gattattttc ctatttataa ggagaagtaa acatggcagc aattaagaaa
    26461 ctctacgatg ccgcaaacgt ggctctggat gttattgatg atgaagtagc aaaaggcttt
    26521 cctgaacctg attgggcgca tcagctacga aacgctatcg cagaaatgac cccaccagat
    26581 ccaacccccg acgagacaga ctggcagcga ttcatccgta tgtacgctca ggaaataggt
    26641 ccaacgccaa cggcagagca ggcaatgctg ctgaaatact tcaaagaggc gggagaggat
    26701 ttaccaattg atgactcagc atattggttc cactgcgcat ggcgtaagta tgacgtgata
    26761 ttcacacaag gcatgggaag caaagatatg gttgtgtggc atctactcca tatagacaca
    26821 gccgttgaca gagttattga acagtttttc cctaaccaag aagattgatc gcctattcat
    26881 aactaacaaa ataagtaaac actaaccaca aaaggaaaaa cacatgagag ttttagttcg
    26941 aatcgttacc agcactgtct atgacgtgtt tccgcttttt atggtcaaag tcgatggcct
    27001 taacgatgaa gaaactgacg cgctgatcca gcgtactctt gttgaatata caggtcatga
    27061 cgctgattca gtgatggttg atgatgatgg tgtttgttgg cataacggca actgttggta
    27121 cgtagaagag actcaacaaa tcagtaatga agatgctgaa catcttgagc gtattttaag
    27181 catcagcact tttgagtgag tttacagtaa agtttatata agttagtatc tacctatcat
    27241 gaagattttt attgaatact tgttactcat cgtttcaata gcttttgtca tcgactgcat
    27301 tttcactggt gtcattcgta aagtcttttc cccggtgcac gacgtagtca taaacgcttt
    27361 ggctatcgtg ctcgtattta attcagcatt tgatgtaatc aaagaggtgg cagcatgaag
    27421 accatcccat tcgcgctgtt gtttctttct tcgatcgttg tggccgacac cactgtttat
    27481 cagtgtgaaa tgtctgtagc cgacgttaag aatggcgctc ttaccgacgt cataaaagca
    27541 ccatatggag cgatggtcgt agacagcggc gaccagttct atgttgtgcg tgacgatcga
    27601 gtgttgtcat ccccatatct cacaaatcgt aatggcaaat taaccggcgt cggagaagac
    27661 cacttcgtat acaacaaata caagggcttc tatggcgttc acgcttctca gcaaagctac
    27721 cttttcgatg actgcaagga ggttgggtaa tggcattaac actggcaggt ctggaaatcg
    27781 agaaaacaag cggctactgg cgtgctaagg gtttcaagca gcctggcatt cttgagcgtc
    27841 tggaacgtga agatgggtat atcgtccacc agcggcgtga atggcgtatg tacgatccag
    27901 aaacaggaaa actgactaca aaagccggta cactttgggg tctgttaaag aaaatacact
    27961 aaatgcaaac tgactgcggc acgttccgca gtcatatttc atagtcgtca ccgctgacag
    28021 catacacaat caactaccgc tgatagcata tcgagagtct atctcaccgc tcacagcata
    28081 ctttactcga ttttttaccg ctgacagcat acttaagaca ttgcatgaat aatgtgtacc
    28141 ggtatgggta taaccagaac aaaattaccg ctggcagcat acgaatgtct gacatatgcc
    28201 attaattacc gctgatagca tatccaaaca aaaattcctc aataaaacac cgctgacagc
    28261 atacgttcta tcagggagta gcaggcaata aatgcctttc actacaagca atcagcgcaa
    28321 tagcaataga atgttagtga gcgcaaacct atatggaatg cactcttcga ggttagtaac
    28381 cactggggag gtatgacaga gcattgagtg gtgatagatg atttaccgct cacagcatac
    28441 gttcatctca ctataccgct ggtagcatat ctttaaccgt tcacagcata cttttcagaa
    28501 aaatagccgc tgatagcata catttcaccg ctgacagcat atcaaagcag tttgagacta
    28561 ttggaaagga tctcaatcat cttgatattt tcaggcgtca aattctgcga aagttcggtt
    28621 atcttgttga taatgttctg tttggcatca atttccccag ctttctcatc tggttttttg
    28681 ggttcgatgt cttcaggttt tggcggtgcg actttgagtt ttggattgcg gctgtgaatc
    28741 tggatataga tcgaccgccc acgcttaatc tcgctgtatt cgagatagcc caaatcttgg
    28801 agagctttta agccgttacg tatagtctga ttctgcgagc tgacattcct gctactcaaa
    28861 ttgagtcgcg cacgcaatcg agcaagcgat accggcgcag gcttggttgg aagactttcg
    28921 atgaaggtgt acagagcctg tgctgtttct ttgcgtggta gcttattgat aacctttaac
    28981 tgcaaaagaa ccttatggtc aaagcgatat agttcggcca gcttcggttc tgcatagaac
    29041 accaccgtat ctttctgctc gttgtagtcc acgctattga tgaggtgcac catcagaagc
    29101 gagatcttgt tagagccgtc gacgttcttt tcttcatacg ttctctggaa agacagagtt
    29161 gtacgcatga tcttcaaaag actgtttgta agccggtcgc ggagtgtttt gcggatctgt
    29221 gacgatggat agccacaaaa cttagcaaat ttcgtgatgc ttaactcgac acgaccgtta
    29281 ggttcgccgt attctgccag cgaacgcaca acgcccaccc acgttttgaa atcatgatcc
    29341 atgtcgagac gaggaccggt tatcttgata tcggaatagc cttcagaacg ggctacttcg
    29401 agctggacaa gctcctttga agcatcgatc tcatttggct tgttacgttt actgtatttt
    29461 gttcccttaa gcgtggggac gaacaacccc agtcgcatca acgcaattgg ttggactgta
    29521 ttgttgctat tagggacaag ttcccctgtg tacaattcaa gggaaccttc ttcaaagttg
    29581 tcgatgttat cttctacttc tttgttattt ttaccttttt tattttttgt ggacatgtgg
    29641 acacctttgt cattcaaccg ctgacagcat acttgatttg ccgctgacag aataccaaaa
    29701 acagttggca gcatacggtg aaccgctgac agactatcaa ttaccgctga cagcatacat
    29761 gaacatgact tcagaccagt cgtggcgcgg cttacagcga tcggggatct tatttgatct
    29821 atataaggat ctatctatgg atctctttat taggatctat cctgtggata tgtgaataat
    29881 taaaacaggc atttactacc ttcggcgcac ctggtgggtt atcgttgcct cggctaacaa
    29941 tcacagaaaa atgacatatg gatctaaaac gcacgcgctg ggttcgtcgt cttgaagacg
    30001 gctcctacac tatcgaatca aattccaacc tgaataagca gaagttgctt tgtgacatct
    30061 gcggtatagc ggcgaagtgc ccgatctacg aaaccagaat taaacttgat aaggctggtg
    30121 tgaattttca tttaaacagt tgcatcaggt acgttccatt actcgcattt cgtaaaccga
    30181 tcatcggatt ggatgccccc tacttcaaca cactccgttc aggtgtgacg tggcgagatc
    30241 gtttatcacc agacaagctg atttgcctcg tatccgcaga cacaggaaaa atcatccgtt
    30301 ttgggaaagt agacaaggtt tactcaggcc cagtagacga aatgttgcgg aaacacagcc
    30361 ggtttaatca tctctgtatg ggtggtgaga aaatcgagaa ggtagaagaa gtgatccgca
    30421 aatcctacgg acactttctg accaaagata gcctgctcac cgcaatctac atcagacatg
    30481 taaaacgtga gttcgacctc gaataccaca gcgaagaaga gcttaacctt gttgacccac
    30541 gtccaaaagc tggcgtcata agcataaacg cagcgcgtaa aaagcccact gacgcgctgt
    30601 aaccctccag atcgtatatt ggcgtagata gaatctacgc ctcctcaaaa tagctctcat
    30661 agcgttctac agtgatcctg tcttattttt agtcatacag acaagcaaag ttgcgccaca
    30721 acaaataggt atatacttac ttataaattt tgtatattaa gacgctcgtt tcattcctaa
    30781 cataccgtta tgcatagttg tttaccttct cattgctctt aaaatttgta tcaaaataac
    30841 cacaaaggaa aaacacatga ctttgccata cggcgtcatt tctgactgcc actaccacaa
    30901 atgggatgcg ttctccacga cgaacgcaga ggggcttaac tccagacttg aaatacagtt
    30961 ggaagcaacg aaagaagcag ccatcgccat gaagaaggcc ggttgtaagt acatgttggt
    31021 tgccggtgat acatttcacg tccgaggaac tgtgtcccct tctgttttgc attacgtaac
    31081 tgaaacgtac aagtggatta tcaacgagct tgatctgaca gtagtaatgc tggccggtaa
    31141 tcacgatctt gaaaccaacg attcagtata tagcgccaac gcagcagcat cgctgagttc
    31201 tatcggcgtg gtaatcgtat gtggcaaacg cccacactca ataaaaattg gtgatgtgac
    31261 tgtccacctg attagctggc gtaacaatca tgcggagctt atcagcgatc tgaaagcatt
    31321 acgtaagagc gtagaaggtg ataatcatga cgttgttatc catacatcca ttaacaaagc
    31381 cattccaaca atgcctgacg tcggtatcga tgcgcaggag ttaaaggata tcggctttcg
    31441 tctcgtgctt agtgggcatt accacaacca caaagaggtc attcctggag ttatcagtgt
    31501 cggtgcactg acccatcaaa attggggaga tgttggatct ctggctggtt acatgatcgt
    31561 aaacccggac ggcagtttca gtcactacga aaccagtgcg cctaaattca ttaacctgga
    31621 agatgatgtt gctgatgacc aaattcgcgg caactacgtg cgtttccgcg ccgtaattga
    31681 gaacgatgaa gaaggcatta agtaccagaa catcctcaaa acaatgggcg caaaaggtgt
    31741 cgtgtgcaac ttcatccgta agtcatcaat gatggaaggg acagccagca caactgaaac
    31801 cagcaaaatc gatagcctgg gagagtcggt atctgcttat tgcaagattg tccacgatac
    31861 tgacggcgga tttgatctga gcaagttgga tattttgtgt caggaaatcc tcaccgaagc
    31921 ggagagttcg gaggctgtgt gagacaaagt cgttatggga gctttcgaga ctttgccatc
    31981 acgatgaaaa gacttgaacg aggccagacg gtgatgtttc acaagcccta cccgccacaa
    32041 ggaaatcccg tagcgtttta tcttggaagg ttaaccagaa aaggcgtatt gaggcgcaga
    32101 tccttcccgg cgcatacgga gttcagattg aaagaaggtc aaaagctaac acacggtatc
    32161 aaaggtgtta tatgaagttt ttaaagctcc aggttgagaa ttttatggct atcgccagcg
    32221 cggaggtcga gttagatcag cgtggtttag tgctcattca gggtgttaat agtgatgata
    32281 gttccgcatc aagtaatggc tctggaaagt caacgctaat gaatagcctg atgtggtgtc
    32341 tttatggcga aacagctcat ggtgtgaagg gtgacgatgt gttgtctacc gaccatgaaa
    32401 agaactgtcg tgttgcagta accatcgagg atgaaggcaa gagatatgcg atcattcgtc
    32461 accgtaaaca caaagagttc aaaaatcggc ttatcgttcg tggtgaagac ggcgatatga
    32521 cgaaaggcaa agatgcgctg acgcaagagt tcgtcgagcg tctgatcggt gcatctaaag
    32581 aggttttcat ggcttccatc tatgcgagcc aagaagctat gccagattta cctggaatgt
    32641 ccgacaaaaa cctcaaaacc atcgtagaag aagccgctgg cgttgacaga ctgacacgcg
    32701 cctacgctat tgctcgtgag cgagctaatg cagctgccgc acgtatggat gtggttaaaa
    32761 ccaaattgga gtcgacaatc tcgaccattg aggcaacaca gtcagaaatt gagtccgcga
    32821 aagcctcctc tgaatcatgg gaacaagagc gttctaaacg ttatgacgat gccctggctg
    32881 ggctggcaag tgccgaagtt gagttaacgg aagttgaact tgagatccgc actcttcccg
    32941 aacagatccg tgataccgag aaggcaatcg aaagtgagcg caaaaagtta gcctcaaaag
    33001 aagaacatga cgccaagttg ctcaaagttc gtggtgcgat aactgatatt cgggcaagca
    33061 tcaaagctac agaaaatagt caggctgatg caatgaaccg cgcgcgtaat tttaagacca
    33121 aagcagaaga ggttggtact aaagtgggat caccatgccc tacttgtggc aaagcctact
    33181 gcgaagaaga tctatcaacg gtgaaggaga atttcattga acaagcacgt caggaaattg
    33241 gtcaggcgaa gacacttgca gaggcaatgg ctaaacacaa aacgaatctt gagaaagcgt
    33301 taagcattga gtctgccctt gttaaaacga cacctgatgt aacggctatc attgcccgga
    33361 ttgaagagct tacgaaacaa ctctcatctt tgcgtcatcg tgagaaggag attgttgcta
    33421 ttgagtctct tgtgactcgt gctcgtactg aggtcgatcg tatatcaaaa gagattaatc
    33481 cgtttattgc tcttatcgcc agacacgaag ataacctggt atccagtaag tctaccttca
    33541 agtccttaaa agatgagttg aaggctattc aggaacaaac gttgctattg gaaaaagctc
    33601 gtcaggtcta ctctcctgcc ggggtgcgtt ctcatatttt gacgtctgtt acgcctttcc
    33661 tgaatacacg cactgccgag tatctcaata cgttgtctga cgggaatatt actgctgagt
    33721 ggtcgacgat ggatgtcacc aaaaaaggtg agtatcgcga caaattcaac attagtgtgc
    33781 agaagaaagg ttcaagtaag tcgttccaga ccctctctgg tggtgagaag cggaaggttc
    33841 gcattgcgtg ttctttggca ttgcaggatc tggttagtaa ccgggcgagt aaaaacatcg
    33901 atttgtttat cggcgacgaa attgacgatg cactcgatac agccggtctt gaacgcctca
    33961 tgggtattct ggagtccaaa gctcgcgagc gaggtactgt gctgattatc tcccataaag
    34021 agatgaagtc atggttccgg gaaactatta cgctggaagt taaagagggg cgcagctatg
    34081 tcgtttaaat taagccgctc gcagtttttg caggtatttg cagtgatgca gtcgataaaa
    34141 ctgatcaatg ggcatacttc caatggtgcg gctccacgta ttctgtgggg cagcaacaat
    34201 attgacggag tacaatttgc cgcgttgctt ggtctaatat ccgagacgcc attgatgcaa
    34261 agtttgaaat cactaccacc tggatgtatt gcgccgatcc tgattaatcc ttttgttgag
    34321 gggggatatc ttcccaacgt cgggcctggg tttattgcat cccatgaaaa tgaagatctt
    34381 aacattaata gcgaagggtt ctttggggga atgggtgcgc atcactgtat ggctttcacc
    34441 aaccttattc gacttgccaa taagcgggtg gatagtttgg catcgccagg tgatgctttt
    34501 actggtttcc ttatccaaag gagggataaa aagtacagtg cggacaaact acagtttgtt
    34561 ggtaagtatg gagaaatggt agaaatcgaa cttcagctcc cgcatgtttt agcaaacgat
    34621 agtgcagaca gtcggaggct gttgggcatc atgcgtcatt tcatagcaag tggcgttaaa
    34681 catgccgtag ataaacgtgt cacgcaggaa aatgagtatt cagactttgc aaactctccc
    34741 caaccaacgt tgcaaacggc aatagtaacc aattcgttgg aggcgagatt attggaaaac
    34801 cctatatggg gaacatggta aggagactat atgagtaaaa aaatcagcgt agttggtgtt
    34861 gatccctcaa tgagcaactt tgggcttgct gtgggcactt tagaccttga aacggacgaa
    34921 cttgagattc acgaccttac tcttgttgag actaaagcgg ggagtaacaa aaagaccgtt
    34981 cgtgtgaaca gtgacgatct gcgccgagcc agtgaaatat ggcgtgttgc gaagccaatc
    35041 attgataagg caaatatggt tttttgtgag ctaccggttg ggagccaaaa ctctcgttcg
    35101 cagacgtctt acggtatttg tatcggtgta cttgcgtgtg tggataagcc attgatccag
    35161 gttactccaa acgaaatcaa gcattttgtc ggcaataaac ttactacatc gaaagaagag
    35221 attatccagt gggctacgaa aaaacactct aaagcaccgt ggctgcgtcg taagcaatct
    35281 ggacaggatg ttctcgtgaa caaaaacgaa catttggctg atgcggtggc tgccatccat
    35341 accggtatgc aaacagatca gttccgccag gtgcgcgatg ttcttaagtc tctcatttga
    35401 tttcattgat aggtaagtgc ttatctatta acatgggcca ctatatttag tggccctctt
    35461 tatttggtga tacatgataa gcatcgtaaa acgtaacggc caaacagagc cgttatccga
    35521 agaaaaatac aaccgcgtcg taatgtatgg cgtagaaggc attcgtggtg taagcgcatc
    35581 cgctgtagca atgggagctg cggccagcat ttttgatggg atgaccacca gccagttgca
    35641 tgaggctttg gttaaatctg ccgctgattt gatctcacca gaagcaccaa attactcaca
    35701 ggtggctgcc cgcctgaaca tttttaaaat ccgcaaagat gccttcggtc gttacgacta
    35761 tccgaacttc taccaacaca ttgtcaagaa cgttaacaag ggcgtttatg acaaggattt
    35821 gctgacacat tattcgtttg aagagatcga agaactcggt aattacatta agccgaaacg
    35881 tgacgatctt tttggctatg cagctacggt gcagttgcaa agcaaatacc tcgttcaaaa
    35941 ccgtgttact ggtgagattc atgaggggcc gcaacatatc tatatgctgg taggcatgtg
    36001 tctgttccag aattgggaag acgactgcgc tggcaaaaca cgtatggaga tggtcaaagg
    36061 tttctatgac gttacaagta cgttcaaact gtctctgccc acaccaatca tggccggcgt
    36121 ccgtactcca acccgtcagt tctccagttg tgtgctgatt gagtctggcg atagtctgaa
    36181 agggattagt gcagcttcag ccgcaattat cgactacgtt tcacgtcgtg ctggaattgg
    36241 tattggtttt ggccgtatcc gtgcgctggg cagcgagatc cgcaatggtg aagccaccca
    36301 taccggagtt attccattcc tgaagcattt ccagacggct gttaaatctt gttcgcaagg
    36361 tggtgttcgt ggtggcgcag caacagcgtt ttacccgatc tggcatcttg aagttgaaag
    36421 tctgctggtg gtgaaaaata accgtggtat tgatgaaaac cgcgttcgcc atcttgatta
    36481 cggcgtcatg agtaaccgtc taatgtaccg tcgactcgtc agaagcgaga acatcactct
    36541 gttcagcccg catgatgtgc ctgatatgta cgaagccttc ttcacagacc aggagctgtt
    36601 tgaaaagctg taccataaat acgaagccga tgattcaatt cgcaagaagt cagtacctgc
    36661 cattgagctg ttctcatctc tgatgcagga acgagcgtcc acgggccgaa tttatattgc
    36721 gaacgtcgat catattaatg agcatggcgc tttcattcct gctcttgcac ctgtccgcca
    36781 gtcaaacctg tgtatggaga tcaccctacc tacccgtcca ctggcattta ccgacgaccc
    36841 gaacggtgag atcgcgctat gcactttatc cgcttttaac ctcggagcca tccgttcact
    36901 ggagtctctt aaagaggtgg cgttctatgc cgttgctgca ctggattcgt tactggatta
    36961 tcaagactat ccgatggagg cagccgaagt gcctgccaaa gctcgtcgta gcctggggat
    37021 cggtgtaacc aactttgctt attacctggc aaagaatggc attcgttatt ctgataccgc
    37081 tggtaataaa ctggtgcatg aaacgttcga agctatccag tattaccttc ttgatgccag
    37141 ctgccaactt gctgaagcaa aaggtgagtg tgactggttt gagcagacca agtacgcaat
    37201 tggtcagttg ccgatcgacc attaccgttc ttcattagac gaaagtggcg aaaccaactt
    37261 tgagttaaag atgccgtggg aagaactgcg tgaacgtatt gcaaaatacg gtcttcgcaa
    37321 ctccacactg acggcacaaa tgccatgcga gacttccagc cagatcacta attccacaaa
    37381 cggcatcgaa ccgcctcgtg gaccggtgtc ggtgaaatct tctaaggacg gcatcgttaa
    37441 gatggtcgtg cctgagattg aaaaactgaa ggaacagtat gaatacctgt gggatatgcc
    37501 ggacaaccgc ggctatctga caaaggtggc gatcatccag aagttctttg accaggctat
    37561 ttcggccaat accaactatg acccttctcg ctttgaaggc gataaagtcc caatgatgac
    37621 gctactgtca gatttgcttc tcgcctacaa gatgggagtt aaaacgcttt actaccacaa
    37681 caccagagat ggggcaggaa agcgtgatga cgacgaaccg cagaatccac tgacgcaagc
    37741 tgtagccgtc gagccagaag atgagtgcga cggagcctgc aaaatctgac atatggtggg
    37801 gtgtatcccc accttctctt tgatttgtaa gccttgttta aacaaatagg ataacaactt
    37861 gtttaaacac atcaaaaagc aaaaggaaaa acacatgtca tattcaacgt tccgtttggg
    37921 tgctaatgat gcaaccaaag agcctatgtt cctcggacaa tctgtcaacg tggcacgtta
    37981 cgatcagcaa aaataccgtg attttgaaaa gttgattgaa cgtcaattgt ctttcttctg
    38041 gcggccggaa gaagttgata tttcgagcga tcgtatcgac ttcaacacga agctgcggga
    38101 ccacgaacgt cacatttttc tgagcaatct ccgttatcaa acgttactcg attcagttca
    38161 gggacgtagc ccaaatgcaa cgctgctgcc gcttatctct attcctgaac tggaaacgtg
    38221 ggttgaaaca tggtctttct ctgagactat ccatagccgc agctacaccc acattattcg
    38281 tggcatggtg gacgatccga gcattgtttt tgacggtatt gttacggatg aagaaatcat
    38341 caaccgagcg atcagtatct ctgctgaata tgacaggctt tatgggatga cctgcgagcg
    38401 ccagtcgtta ggcgagaagg aatttgagcg tctgtacgta aatgaatatg gctgggagcc
    38461 atatcctttg caccgtcagc ttttccgcac gttggtgtcc attaatgcgc ttgaggcgat
    38521 ccgtttctat gtaagttttg catgtacgtt tgcctttggc gaacggaagt tgcttgaggg
    38581 taacaccaaa attatgcgct ttattgcccg tgatgaagct ctgcattgcg aaggaactga
    38641 acgcatgatc cgcttcatgc gtaccggtcg cgaaggttta ttgtggaaag agattgctgc
    38701 tgatgaagaa aacgtcattt acgacaccat gaaatcagtc gccgaacaag aaatgaactg
    38761 ggccgagtat ctcttcaaag acggttcgat gattggttta aacgcggata ttttgaagac
    38821 ctatgtaaaa taccgcacca atctggctat gaatcgtctt ggcctgaagg ctttatttcc
    38881 agaggttacc accgatccgc tggtctggat gaacaagtgg ttgttaaccg acacactgca
    38941 aattgcacca caagaggcag agcaaagcac atatctggta ggtcagatcg attctaccgt
    39001 ggacaaggct tctctaagcc agtttgcaga cctgtgaacc gatacaaagc attatgtaac
    39061 ctggcaacgc tgggccacaa tggatcacaa gaattaagaa ggaacaaaac tagcatgaac
    39121 tttaccaaac tgactgacca cctgaaactt gccaccgatc gtctcattgg atttaagcca
    39181 gaaccatatg agttgcatga aggtcatggt gtagctactg aaagtattta caagatggtc
    39241 gatcagtttc atgaactctt ccagcatccg agacgcgtta tgccgacacc agagctgctt
    39301 cgtctccgtg caagcctgat tcatgaagaa gctgtagtgg aaggtattcc agctgcaatg
    39361 aatggggata ttgagcaact gctggatgca atggccgact ttttatacgt tggtgttggt
    39421 acgatggtcg ccatcaaagg tggtatttct accggcatga cctattacac gcaggaacag
    39481 agcattgatc gctttatgca gacaattttt gtgcccggta acactgtttt cgatgatatg
    39541 gcaatgccat ttcaggaagc tcgtgaggcg tcatgtatgc tcgaagagct ggcagataaa
    39601 cttgagaaca agactgttaa ggattctgag ctgattcagg aactgcgccg tgtcatgaac
    39661 aaaatctatg tggcgtgcat gatgacctat cgactggctg atttcctcgg tatcaatgta
    39721 gtcgagctgg ttggcgaaat tcatcggtct aacatgacaa aattatggcc tgctgatgta
    39781 gaggaacgtc gccaggctgt ggccaactgc aaatacgact cttcagacct gggatttcgc
    39841 catgctgatg gcaccgataa gatgatcggt tttcgaattt ccgatggaaa gattctgaag
    39901 tctccaacct atagtgatgt cgatttatcc tcctttgttg agcaagctaa agcctcagca
    39961 atgtacggaa tgatcaaaaa ataattgtag gtgattatct actaatgtat atttgatttg
    40021 cgtataaaac tctggaacga ctattctttt tggtggccta tggccaccat tttttatcta
    40081 tctggtcttg ttttatcaat gaatgtaaac tcacgtgata aataattgat tacttatctt
    40141 cgtgaggttt tatgtcactc cttttgaatc gtgagcatac gaacggtcag gtaacaaacg
    40201 catcgtatgc aaaagttatt gagacggtgc ttaaaagcgg cgtgcagact gatgatcgca
    40261 caggcactgg tactttaagc acctgctacg ttccctctta ctacatgctt actggtggga
    40321 ctgtgccgct tatttctgga aaggcggtaa atcttaagcc actgcttgtc gaacttgagt
    40381 ggtatctgaa aggcacgggc aacatccaat ttcttaagga taacggcgtt aagatttggg
    40441 atgcatgggc cgatgagaat ggcgatttgg ggccggttta cggtaagcag tggcgtcgat
    40501 gggaagatac ccgcatcgtg agccatagtg aatatctgag caagatcgct actttccgtg
    40561 aacgcgggta caaagtcgag ggatacctgg gtatcagtga agatcgcgta gtgctgtccc
    40621 gtgaaatcga tcagctacag cgtattgtcg acacactgcg cacgaaccct accgatcgtc
    40681 gcatcatgct taacgcatgg aacgtaggcg agcttgagga tatgaaactg ccaccttgcc
    40741 actttgtctt ctctttgtgg agtcgtgagc ttgattttga aacccgttta acgatggcaa
    40801 ctgacattgg tcttcaacac agtcgcttcg gttatgagtc tatctacacc aagatgctat
    40861 acgatctgga gatggacggc agtgttactg aaactgaact ggatgaactt ggaatcccca
    40921 aacgcatcct caactcctgc ctcgtacagc gtagcgtaga cacttttgtt ggtatgccat
    40981 tcaatattgc tggctatggc attctcactc attttctcgc gaagattacg ggtcacatgg
    41041 ccggtgcatt tgtgcatttt ggctttgacg tgcatttgta caacaaccac atggaaggtg
    41101 tgtgtgagct aatgaaacga caggctccag agcattcaga tccggtcgtt attttccctc
    41161 atgaatggtc tgagctggat gatttcaaat gggacgatat tctgattctt ggctatgacc
    41221 ctctaccgtg gatcaaggtt ccagtggcgg tgtgatatgg caagaggtat gtatgtctta
    41281 tgcgaaattg aaggtgtgct ggcaaatacc agccatcgta aatctgtacc tgacacggat
    41341 gcaggccagc tcattgccgg tgatgaactc attttcccca ccagccgtat gttgcgtggt
    41401 tttgctcgct caggggctga agtggtgctt atcagtagcc gctctgaaac tcttgaagcg
    41461 cccactaaac gatggctgaa agattttggc gttgattacg actggcttca tctcgtaccg
    41521 aatggcacca gttatgagaa gcatattaag cgcacattag cggagcataa aggcgatctg
    41581 cttatcgctg cgctggtgca cgatcctcga ctccgtgccg ctttagctga ctctcatcat
    41641 agaccggtca tctatgaggt gagcaaatga agatgatagc tgctgttggc cgtaactatg
    41701 agatcggcat agcgaacgaa ctgccctggc gttgttctac cgatctgaag ctatttaaga
    41761 gactcaccaa aaacgccact gtcgttatgg ggcgtaaaac gatggaaagt ctcaaacgcc
    41821 ctcttccaga gcgtcataac ctcgttttga cgcgctctca tggctttgta ccaaatggat
    41881 tctaccctgc tggtgtggat gatgtgttgc gattaccaga gcctgtgtgg gtgattggcg
    41941 gggaacaaat ttactcgcta ttcatgccgc atgttgaaga gatttggctc tcccacatcg
    42001 gcgttgatgt gccaaacgcc gatgcattct tcccggcaag catgatgcgt aatttaggct
    42061 ttgtgcctgt tgaaacagtt tttacccaac gagccagcga ggaagagcct ggcttttcgc
    42121 agatcgtata cagaaggtcg taatggatta ccggattggg atcactggtg ctcagggcag
    42181 tgggaaaaca accctggcaa aatatatcga caaacattac ggaatccctt acgtggatgc
    42241 tggtgtcgga agtttgatga gccgactcgg tgttcgagta ggtgagtcta tgcctctata
    42301 tgagcggctt cagattcaaa tggaaatcgc aaagcatata gagctactta cgcgtggtgt
    42361 tgaaggcttt gttatcgatc gcacacctgc tgatgttatg gcctacacgt tggatttggt
    42421 cggccatacc aatgaagatc gatgtattga gttagccctc gatatcgaaa agttttgcca
    42481 caaaactgct atttcaaact ttaacgccat tgctggccta cgcccgggag tcgctctctc
    42541 agagcgagat tacttgcgat cacaacgagc atcattagac cgtctgtatg tcgctcgtat
    42601 tgatgcgttg atgtgcgggg aactgacaaa aattcacctg catccgcaaa ggggggatct
    42661 gcaaaccttc gtcgtttcca accgatatcg cacggttgaa gcaagagcca gatcagtgat
    42721 gagaatgcta gataacgctg tagaaaagat agaaaaccgg ttctgtggtc gagtgaccgt
    42781 tcattagaaa ttgttcgcct cttcgacatt gcgacaataa aactctcaaa atgggttaag
    42841 gataaaaaat gtttagtgaa atgttgcttg aagatgaact ggatcggaaa acaacagagg
    42901 ctttgattcg tgtagcggac gaacattccc ggtcgcttat gagcgatcga gaggctcgtc
    42961 tggctattcg tgccatattc gaaactgcgc aggggcttgt tggcgcacaa gtaggtgaag
    43021 ccattaacat cgccatgtct cagttcagtg aagacagtaa aaagcctctg tttcctatgc
    43081 atttgatgct ggctggtggc acggtgcttt atatctctgt ttgtctggat agcaaccaaa
    43141 tcaatattct caacactgcg tcaggtaagt ggaaagatcc gattgtctgt gaaaccagtg
    43201 aagaaacttt gaaaaaagcg gctcaatttg tacgtagcgc actacttaag ggcgctaaga
    43261 agttgtaagg agttctgatg acaacgattg ttgcaggcat cgatatcgag tctacgggac
    43321 tggatttcct tgctggtcat aaaattattg aaatcgcaat tacccgctat gaactggaga
    43381 cacagagaca tattgatagt ctggagatgc gttttaaccc tcgcagaaac atagatccga
    43441 aagctcaagc cgttcatggc atttcattag aacagctcgc agctgaacct ttgttgtcaa
    43501 atcatgctag cgaaattggc gcttatatgg aggcatgtag tgcgtgggtt gctcataacg
    43561 gcgaagcgtt tgatatacca tttattagac acgagttttc agggtatgga gtaagactgc
    43621 cagacgttcc cgttatagat actatgttat cgggattgtg ggccacagaa gacggtaaac
    43681 gtcctcgcct tgaagaattg gccttctctc ttggctttat atacgatcat gccaaagcac
    43741 atagtgcctt atatgacaca aacttaatga tgcaatgctt ctttaaggca cgtaataaat
    43801 acggattttt taaattaccc tctgaaattg tgtaaaacaa aagcctgctt taaagagagt
    43861 ttgaagcagg ctttctttta aagaacagtc gcctttcaat catttcctgc ctgtatttaa
    43921 tacttttccg cctgataggt ttagtcaaaa tgtagccatc gaaacgcaaa tgcaactaaa
    43981 cagaaggaga cttacatgag ttcggttgaa aatgtaatga caaatgatga tctggatgag
    44041 ctgacagcca tgttgcaatc acttgatgaa ccagtaaaaa aagctgcaca ggttgaaaat
    44101 actgatgata ttgacgatct gcttctcggc ctcgatgctg gcgtagccat gagttctgat
    44161 gatgttgccg aagaactgtt caatgaagaa aaagcaggtg atttcagctc tgctttaaat
    44221 gagttggagt tagcgcatga gcctataaac gtaattaacg ctgaaagtgg tgaagctgcc
    44281 gaaaatgagc cagaacagtt ggggatcatt gaggttgaag attgtgttga ggttaatgat
    44341 gaattaaaag ttcaacagtc aaatgatagc aatacaaata aaaaaactcg tgctgcaaga
    44401 ggtcctagtt ttactttaag tgataaagat gattcgtttt tcaataaagc aggcttagaa
    44461 aaagatattt tcttagacgc ttacgaaaac gcgcctgtca aagcgaagga taagatatta
    44521 aaccttctta attggtttag cggaggtcca gatattagtg tttacacggt aatttccatg
    44581 agacaccttc ttacagaaaa gaaggctaca agtaatagta ttaagattgc tttaatgagc
    44641 aatccagaaa aaccgtatcc gcttaacact gcgtcaactc aggctgggca aatgatggct
    44701 gtatttccag ctacaggaat tgccgttaga gacggtggaa atctaacatt gaacgaagaa
    44761 tcaccgatcg ttaagaagtt tgtcgcggag tacactattg gatgacgttc ccctactgaa
    44821 aataaagccc atagagagct ttatagtgct gggtaagata acccataccc agcaccaaaa
    44881 aaacgcgcca gagagcttct tgttcgcatt tctggcgcgt tttatttgat tgccatacat
    44941 aaaatcaaat gcaaaaatag gtatttactt acctatcgag aaagaagatg attgcagccg
    45001 aaaaaatcaa acagcgaaag cgcgacaact ctcttcgtga cctctggaga acacctgatt
    45061 ggctgttttc tgccattcaa cgttatcttg gagtgacatt tgatgttgac gttgcctgca
    45121 acaaggacaa cgcaaagctg cctaatttca taggcattga gcgtgatgct ttgaaatctg
    45181 aatggggaca gccaggtaca attgccttcc tcaatccacc ctactccaaa atctccccct
    45241 ggattgatgc tgctatacgt gagcaggctc gcggagttac tacagtgatg ctaattcctc
    45301 aatccctcga tacaaagtgg tatgagcgtg caacagagta tgcgaatgag acgattattc
    45361 tgtctggtgg ccgcgtagcg tttgtcgagc ctgacgtcaa tttgggtcag gtagaagtaa
    45421 acatcaaccc cggtggcagt atgctcgttg tttttcgagg attctgtcag gacgctgggc
    45481 actctataag caagatccct ttggacgtca tgaaaagtct gggagggtat gatcctgcga
    45541 atgtgatcag gaaaaaaaga ccatcaaaga aggctgctta gtttgttctg gcgtctgtaa
    45601 ttagcctgct tctgtatata tataaataac tacatattaa ttattaatat acggaagcag
    45661 gctgttttgt atcagagact ccagacctga acatcactac agaatccact agaccccctt
    45721 cccagacgct ttaaaatcga ttttatgaac cactttaagg aaaccaacat gtcatacccg
    45781 actaatgtcg ttgcgctcgt agacagcgat tttctggccc aggctcgtga aatgatgaaa
    45841 gatcgtgagc aggctttcaa cttgtacgag tgggcaatta agtgcttgca tcttggggag
    45901 catcgcgaac ttgttgaaca gcttttaggt gagttgatca acgaggtgtt tgccttgaat
    45961 gttcaactac atggtcgaga aaataatcaa tcgaaatgat agataagtac aaactattca
    46021 taagatgaat tgtaagtgct aagatctgat agtttccagt cgtagactgg agactcgacc
    46081 tgatgggtgg gggtaagcgt cactggcgtc aggtttaaaa aagctcacta ccagcgtaga
    46141 accggtgccg tttaggtgtc ggggaagggg gaaccaaagt gagcggagac aagggtcact
    46201 ttatgattgt cgagtctggg gtgtttcgag aggttgaatc cagtactccc cttcataaag
    46261 tgtgggaaga tctcggttct ggggtgctgt catccataac ttcccaagtc taagctggca
    46321 gtagacttag accatagctt ttcaggttat gaaacgacca ggttggtgag gaattttata
    46381 ctcacctccc tgggagagta ttacctgaaa agacaacctc tcacttcgtt cgaggtgaac
    46441 ttcactcact tcgttcgttc agttcaggtt tataaaaacc tgttctggga agtaatttgt
    46501 ttattttaat aattattaac acgcacgcgt gtgcgcacgc gcgaggaaaa atcggcgcgg
    46561 cgcttgattc aggagtttat atgacaacga agacaccagc ccgaccgcaa gcaaaaactc
    46621 gcaaaaaaga caaaaacaaa aattctcccc gcaccaatcc cacaacgcct gtcgtagagt
    46681 tcaatcccca gcttaaaacc gtgaaaatct ttagtgatgg ctcttgcctt aaaaatccgg
    46741 gtggcccggg cggttacggt atcgttctcc agtatcgtgg cgaggaacgc gagttctcag
    46801 atggttttca tagcaccacc aataaccgca tggagatgat gggggcactt atcgggctgg
    46861 agcgtttgaa atatccatgc aacgttattt tgcactctga tagccagtat ctgaaaaacg
    46921 gcatgacaca gtggatgaaa tggtggaaac gcaatggatg gatgacttct gacaaaaaac
    46981 cggtaaagaa tgttgatctg tggaagcgtc tggatgaggc cgcaagtcga cataatgttc
    47041 gctggaagtg ggttaaaggt cacgccgggc atcgtgaaaa tgaaatatgt gatcgactcg
    47101 cgaagatcgc agctttttca gcagcagata tgcctcacaa gaaagatatt ggttttgttt
    47161 ataacaaaca gtaagtaagt gtttacctat cattataaat catgtatctt atcggcgtca
    47221 ggatgacaat gtgtcggtaa gacacagttc c
//
LOCUS       DAZOZD010000004        46753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOZD010000004 DAZOZD010000000
VERSION     DAZOZD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.004"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUPB_000162"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUPB_000162"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0757090.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="ACHUPB_000163"
     CDS             973..1341
                     /locus_tag="ACHUPB_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0757091.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUPB_000164"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUPB_000164"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0757092.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUPB_000165"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUPB_000165"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0757093.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUPB_000166"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUPB_000166"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0757094.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUPB_000167"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUPB_000167"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0757095.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUPB_000168"
     CDS             complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUPB_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0757096.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUPB_000169"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUPB_000169"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0757097.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUPB_000170"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUPB_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR0757098.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUPB_000171"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUPB_000171"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0757099.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10636
                     /gene="murP"
                     /locus_tag="ACHUPB_000172"
                     /pseudo
     CDS             9212..10636
                     /gene="murP"
                     /locus_tag="ACHUPB_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUPB_000173"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUPB_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR0757100.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUPB_000174"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUPB_000174"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0757101.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="ACHUPB_000175"
     CDS             complement(12998..13573)
                     /locus_tag="ACHUPB_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0757102.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="ACHUPB_000176"
     CDS             complement(13634..14083)
                     /locus_tag="ACHUPB_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0757103.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="ACHUPB_000177"
     CDS             complement(14070..14495)
                     /locus_tag="ACHUPB_000177"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0757104.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUPB_000178"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUPB_000178"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0757105.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUPB_000179"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUPB_000179"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0757106.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUPB_000180"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUPB_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR0757107.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUPB_000181"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUPB_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0757108.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUPB_000182"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUPB_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0757109.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUPB_000183"
     CDS             complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUPB_000183"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR0757110.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUPB_000184"
                     /pseudo
     CDS             complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUPB_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUPB_000185"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUPB_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0757111.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUPB_000186"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUPB_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR0757112.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUPB_000187"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUPB_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR0757113.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUPB_000188"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUPB_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR0757114.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="ACHUPB_000189"
     CDS             complement(26015..27418)
                     /locus_tag="ACHUPB_000189"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR0757115.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUPB_000190"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUPB_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR0757116.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUPB_000191"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUPB_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR0757117.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUPB_000192"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUPB_000192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0757118.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUPB_000193"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUPB_000193"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR0757119.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUPB_000194"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUPB_000194"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR0757120.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUPB_000195"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUPB_000195"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0757121.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUPB_000196"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUPB_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0757122.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHUPB_000197"
     CDS             complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHUPB_000197"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0757123.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34333..35283
                     /gene="tal"
                     /locus_tag="ACHUPB_000198"
     CDS             34333..35283
                     /gene="tal"
                     /locus_tag="ACHUPB_000198"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0757124.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35303..37306
                     /gene="tkt"
                     /locus_tag="ACHUPB_000199"
     CDS             35303..37306
                     /gene="tkt"
                     /locus_tag="ACHUPB_000199"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0757125.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37402..38445)
                     /locus_tag="ACHUPB_000200"
     CDS             complement(37402..38445)
                     /locus_tag="ACHUPB_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0757126.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHUPB_000201"
     CDS             complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHUPB_000201"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR0757127.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHUPB_000202"
     CDS             complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHUPB_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0757128.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41399..43099
                     /gene="narQ"
                     /locus_tag="ACHUPB_000203"
     CDS             41399..43099
                     /gene="narQ"
                     /locus_tag="ACHUPB_000203"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR0757129.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43263..46376
                     /gene="acrD"
                     /locus_tag="ACHUPB_000204"
     CDS             43263..46376
                     /gene="acrD"
                     /locus_tag="ACHUPB_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0757130.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHUPB_000205"
     CDS             complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHUPB_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0757131.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10706 a        13151 c        12110 g        10786 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt atagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcacctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 tgactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcta
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgtcag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 tggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagagttaac ggaggcaatc acccgcatag
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcatca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctagtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accgggttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acggagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attacgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt attattcgca
    31681 aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg
    31741 gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag
    31801 cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    31861 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    31921 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    31981 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgccg catcgccgtg
    32041 catttcacca tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg
    32101 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    32161 gcgcggttca ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    32221 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    32281 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    32341 tttcaccacg ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc
    32401 ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg
    32461 cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc
    32521 tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg
    32581 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    32641 cgccagtccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc
    32701 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    32761 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    32821 acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc gcaggagcga tcttaacgat
    32881 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc
    32941 gccatacgct gaagccacca cttcgctctg ttccgcatgg gccagttctg caatcgcgcg
    33001 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    33061 gaagatgaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt
    33121 gcagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    33181 attcgccagc gccagaatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    33241 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    33301 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg
    33361 gccttgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag
    33421 cgctaccagc agattcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    33481 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    33541 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    33601 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt
    33661 cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat
    33721 gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg
    33781 tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    33841 cgccaccagg ttaccgcggg cggtatattt gtaggctttt aacgggtctt tttcgatttc
    33901 aagacaaggt gcggcaacgc cgggtgagta ggccagcgcc agatcgcgct gcgttgccag
    33961 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    34021 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    34081 tgtgtgggca acaccccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac
    34141 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    34201 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    34261 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    34321 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    34381 agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg
    34441 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    34501 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    34561 gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt
    34621 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    34681 ttgtatcagc aacaaggcgt tgagaaatca cgcattctga tcaagctggc ttcgacctgg
    34741 gaaggaattc gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    34801 ctgttttctt ttgcacaggc acgggcctgt gcggaagcag gcgtttttct gatttcgccg
    34861 tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    34921 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    34981 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    35041 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg
    35101 ccagtggtac gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc
    35161 gaagcggagt tccgttggga gcacaatcag tatgcgatgg cggtagaaaa actgtctgaa
    35221 ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    35281 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    35341 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    35401 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg
    35461 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    35521 gctgcatctg accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca
    35581 ttcgaaaacc ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg
    35641 tccacttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    35701 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    35761 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    35821 actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    35881 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    35941 tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt
    36001 gaaagataag ccgtcgctga ttatctgtcg tacggtgatt ggctttggat cgccgaataa
    36061 agcaggtaag gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg
    36121 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcctg
    36181 ggatgcccgc gaaaaaggcg aaaaagcgca gcagaggtgg aatgagaagt ttgccgccta
    36241 taaaaaggct catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc
    36301 gaaggactgg gagaaaacga ctcagaagta tatcaatgag ttgcaggcga atccggcgaa
    36361 aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct tatggcccct tgctaccgga
    36421 gctgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    36481 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    36541 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    36601 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    36661 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    36721 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    36781 cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    36841 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    36901 agagattact cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    36961 gattgccacc ggttcagaga tggaaatcac cctgcaagct gcggagaaat tagcaggaga
    37021 aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga
    37081 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    37141 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    37201 ttatggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    37261 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatagt gatcgccgga
    37321 tgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc
    37381 tacatgtccc cgccattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc
    37441 atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    37501 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    37561 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    37621 cccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    37681 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctga tcatcatcag
    37741 cgctttatca tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    37801 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga
    37861 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    37921 gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    37981 ctgagcatca ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc
    38041 gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc
    38101 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa
    38161 cggctcacca tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt
    38221 caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag
    38281 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt
    38341 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctagt gccgcccaca ccaaagacgt
    38401 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    38461 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca
    38521 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt
    38581 aaatgtgacg tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    38641 gttttgatca tctccagcgc ctggctgaac ggcagctcaa gcacttcaat atcttcatct
    38701 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    38761 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    38821 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    38881 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    38941 gcgacacgga actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc
    39001 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg
    39061 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    39121 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    39181 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    39241 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    39301 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    39361 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    39421 ctccagccac ggcatcccgt gtggattgaa gccaaacacc ataatcaccg catcggctgg
    39481 catgacaaat tcactgccct caatcggtac aagtcgccgg cgcccctggg catccggctc
    39541 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    39601 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    39661 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    39721 gccgtggcgc agcgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    39781 gacgttgagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac
    39841 ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    39901 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    39961 ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    40021 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    40081 aggaatgccg aaagtgagca agccaccaat ttctggatgg cgatcgtaca ccgtcaccgc
    40141 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacccg caccgataat
    40201 cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa
    40261 cgcctgatct gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    40321 aatagtacag gcaccttcac acaaacggtc ctgcggacaa acgcgtccgg taatttccgg
    40381 cagggtgttg gtcaggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    40441 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    40501 gccgcattta aggcagcgcg aggcttcccg ttgtgcctga tcggcacgga atggcagata
    40561 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    40621 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    40681 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    40741 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    40801 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    40861 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    40921 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    40981 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    41041 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    41101 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    41161 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    41221 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat
    41281 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    41341 gactaaattg cgttttagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    41401 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    41461 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    41521 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    41581 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcatc
    41641 ggtattaacc aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    41701 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    41761 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    41821 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    41881 tttcacgctg gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa
    41941 tcagctggtt accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga
    42001 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga
    42061 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    42121 cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg caggcgctga acacaagcca
    42181 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga
    42241 atatctggag ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga
    42301 attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    42361 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg
    42421 acgcggtttg tactttaatc aggcgcaaaa gcattttcag cagttattgt tgatggaaga
    42481 acgtgcaacc atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg
    42541 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    42601 catggccgat ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    42661 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    42721 tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc
    42781 gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    42841 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    42901 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    42961 tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt
    43021 ttcaccacct tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga
    43081 ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag
    43141 tataaaaggg catgataatt tacattaact cctttttttc tccacgattg gctcgtacct
    43201 tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt
    43261 taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt
    43321 gtctgacagg gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc
    43381 caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca
    43441 ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat
    43501 ctcagagcag tggcatcggt caggcatctg tcactttaag ttttaaagca ggcaccgatc
    43561 cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc
    43621 aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca
    43681 ttgctttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca
    43741 gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt
    43801 cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg
    43861 ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg
    43921 gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc
    43981 tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg
    44041 taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc
    44101 gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa
    44161 tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg
    44221 gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg
    44281 tggtgaaaac gctgctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc
    44341 aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct
    44401 tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac
    44461 tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt ggaaaacgtc gaacgtatta
    44521 tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg
    44581 gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg
    44641 gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc
    44701 tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac
    44761 tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta
    44821 accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgc agcctgcgct
    44881 ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga
    44941 cgtcgttctt accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg
    45001 gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc
    45061 atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg
    45121 ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga
    45181 ccggcacctc gtttgccatt atcgagcgtg caacaaaggc gtttaacaag attaaagaag
    45241 ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg
    45301 atatggagtt gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc
    45361 agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg
    45421 acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg
    45481 ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact
    45541 ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc
    45601 tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct
    45661 ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt
    45721 attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg
    45781 atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga
    45841 tgtcgtatca ggagcgactt tccggcgcgc aggctccggc gctgtacgcc atttccttgc
    45901 tggtggtatt cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa
    45961 tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg
    46021 aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg
    46081 ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag
    46141 cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta
    46201 tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg
    46261 tgggtactgg cgtaatgggc gggatgattt cagccactat tctggctatt tacttcgtgc
    46321 cgctgttctt tgtgctggtg cgccgccgct tcccgctaaa gccgcgcccg gaataagcaa
    46381 taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca
    46441 gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa
    46501 agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta
    46561 ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct
    46621 ccggggggac aaatcttatg taaatactat ggtcctgcgg tgatgatttg tatgtgatac
    46681 acagcaacat ttcgagatat tcatacggca tctaatactg atttaattca ggttaaaata
    46741 cagacagata aca
//
LOCUS       DAZOZD010000005        45635 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOZD010000005 DAZOZD010000000
VERSION     DAZOZD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.005"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(48..1907)
                     /gene="gss"
                     /locus_tag="ACHUPB_000206"
     CDS             complement(48..1907)
                     /gene="gss"
                     /locus_tag="ACHUPB_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0757132.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2112..2978
                     /gene="yghU"
                     /locus_tag="ACHUPB_000207"
     CDS             2112..2978
                     /gene="yghU"
                     /locus_tag="ACHUPB_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0757133.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3007..3088)
                     /locus_tag="ACHUPB_000208"
                     /pseudo
     CDS             complement(3007..3088)
                     /locus_tag="ACHUPB_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3101..3349)
                     /gene="hybG"
                     /locus_tag="ACHUPB_000209"
     CDS             complement(3101..3349)
                     /gene="hybG"
                     /locus_tag="ACHUPB_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0757134.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3362..3703)
                     /gene="hypA"
                     /locus_tag="ACHUPB_000210"
     CDS             complement(3362..3703)
                     /gene="hypA"
                     /locus_tag="ACHUPB_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0757135.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3696..4184)
                     /gene="hybE"
                     /locus_tag="ACHUPB_000211"
     CDS             complement(3696..4184)
                     /gene="hybE"
                     /locus_tag="ACHUPB_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0757136.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4177..4671)
                     /locus_tag="ACHUPB_000212"
     CDS             complement(4177..4671)
                     /locus_tag="ACHUPB_000212"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0757137.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4671..6374)
                     /gene="hybC"
                     /locus_tag="ACHUPB_000213"
     CDS             complement(4671..6374)
                     /gene="hybC"
                     /locus_tag="ACHUPB_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0757138.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6371..7549)
                     /gene="hybB"
                     /locus_tag="ACHUPB_000214"
     CDS             complement(6371..7549)
                     /gene="hybB"
                     /locus_tag="ACHUPB_000214"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0757139.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7539..8525)
                     /gene="hybA"
                     /locus_tag="ACHUPB_000215"
     CDS             complement(7539..8525)
                     /gene="hybA"
                     /locus_tag="ACHUPB_000215"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0757140.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8528..9646)
                     /gene="hybO"
                     /locus_tag="ACHUPB_000216"
     CDS             complement(8528..9646)
                     /gene="hybO"
                     /locus_tag="ACHUPB_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0757141.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9835..10122)
                     /gene="yghW"
                     /locus_tag="ACHUPB_000217"
     CDS             complement(9835..10122)
                     /gene="yghW"
                     /locus_tag="ACHUPB_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0757142.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10241..11128)
                     /gene="yghX"
                     /locus_tag="ACHUPB_000218"
     CDS             complement(10241..11128)
                     /gene="yghX"
                     /locus_tag="ACHUPB_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0757143.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11285..12325
                     /gene="gpr"
                     /locus_tag="ACHUPB_000219"
     CDS             11285..12325
                     /gene="gpr"
                     /locus_tag="ACHUPB_000219"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0757144.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12365..12859)
                     /locus_tag="ACHUPB_000220"
     CDS             complement(12365..12859)
                     /locus_tag="ACHUPB_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0757145.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13050..13934
                     /gene="yghA"
                     /locus_tag="ACHUPB_000221"
     CDS             13050..13934
                     /gene="yghA"
                     /locus_tag="ACHUPB_000221"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0757146.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14094..14519)
                     /gene="exbD"
                     /locus_tag="ACHUPB_000222"
     CDS             complement(14094..14519)
                     /gene="exbD"
                     /locus_tag="ACHUPB_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0757147.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14526..15260)
                     /gene="exbB"
                     /locus_tag="ACHUPB_000223"
     CDS             complement(14526..15260)
                     /gene="exbB"
                     /locus_tag="ACHUPB_000223"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0757148.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15512..16699
                     /gene="metC"
                     /locus_tag="ACHUPB_000224"
     CDS             15512..16699
                     /gene="metC"
                     /locus_tag="ACHUPB_000224"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0757149.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16839..17498
                     /gene="yghB"
                     /locus_tag="ACHUPB_000225"
     CDS             16839..17498
                     /gene="yghB"
                     /locus_tag="ACHUPB_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0757150.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17538..18437)
                     /gene="yqhC"
                     /locus_tag="ACHUPB_000226"
     CDS             complement(17538..18437)
                     /gene="yqhC"
                     /locus_tag="ACHUPB_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0757151.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18631..19794
                     /gene="yqhD"
                     /locus_tag="ACHUPB_000227"
     CDS             18631..19794
                     /gene="yqhD"
                     /locus_tag="ACHUPB_000227"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0757152.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19899..20726
                     /gene="dkgA"
                     /locus_tag="ACHUPB_000228"
     CDS             19899..20726
                     /gene="dkgA"
                     /locus_tag="ACHUPB_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0757153.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20926..21852
                     /locus_tag="ACHUPB_000229"
     CDS             20926..21852
                     /locus_tag="ACHUPB_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0757154.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21903..22160
                     /gene="yqhH"
                     /locus_tag="ACHUPB_000230"
     CDS             21903..22160
                     /gene="yqhH"
                     /locus_tag="ACHUPB_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0757155.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22203..24422)
                     /locus_tag="ACHUPB_000231"
     CDS             complement(22203..24422)
                     /locus_tag="ACHUPB_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0757156.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24533..25945)
                     /gene="ftsP"
                     /locus_tag="ACHUPB_000232"
     CDS             complement(24533..25945)
                     /gene="ftsP"
                     /locus_tag="ACHUPB_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0757157.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26020..26757)
                     /gene="plsC"
                     /locus_tag="ACHUPB_000233"
                     /gene_synonym="parF"
     CDS             complement(26020..26757)
                     /gene="plsC"
                     /locus_tag="ACHUPB_000233"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0757158.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26990..29248)
                     /gene="parC"
                     /locus_tag="ACHUPB_000234"
     CDS             complement(26990..29248)
                     /gene="parC"
                     /locus_tag="ACHUPB_000234"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0757159.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29794..30276)
                     /locus_tag="ACHUPB_000235"
     CDS             complement(29794..30276)
                     /locus_tag="ACHUPB_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0757160.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30329..30721)
                     /gene="ygiW"
                     /locus_tag="ACHUPB_000236"
     CDS             complement(30329..30721)
                     /gene="ygiW"
                     /locus_tag="ACHUPB_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0757161.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30873..31532
                     /gene="qseB"
                     /locus_tag="ACHUPB_000237"
     CDS             30873..31532
                     /gene="qseB"
                     /locus_tag="ACHUPB_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0757162.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31529..32878
                     /gene="qseC"
                     /locus_tag="ACHUPB_000238"
     CDS             31529..32878
                     /gene="qseC"
                     /locus_tag="ACHUPB_000238"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0757163.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32988..33569
                     /gene="mdaB"
                     /locus_tag="ACHUPB_000239"
     CDS             32988..33569
                     /gene="mdaB"
                     /locus_tag="ACHUPB_000239"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0757164.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33600..33914
                     /locus_tag="ACHUPB_000240"
     CDS             33600..33914
                     /locus_tag="ACHUPB_000240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0757165.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33962..35854)
                     /gene="parE"
                     /locus_tag="ACHUPB_000241"
     CDS             complement(33962..35854)
                     /gene="parE"
                     /locus_tag="ACHUPB_000241"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0757166.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35883..36464)
                     /gene="yqiA"
                     /locus_tag="ACHUPB_000242"
     CDS             complement(35883..36464)
                     /gene="yqiA"
                     /locus_tag="ACHUPB_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0757167.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36464..37291)
                     /gene="cpdA"
                     /locus_tag="ACHUPB_000243"
     CDS             complement(36464..37291)
                     /gene="cpdA"
                     /locus_tag="ACHUPB_000243"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0757168.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37316..37738)
                     /locus_tag="ACHUPB_000244"
     CDS             complement(37316..37738)
                     /locus_tag="ACHUPB_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0757169.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37739..38368)
                     /gene="nudF"
                     /locus_tag="ACHUPB_000245"
     CDS             complement(37739..38368)
                     /gene="nudF"
                     /locus_tag="ACHUPB_000245"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0757170.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38573..40054
                     /gene="tolC"
                     /locus_tag="ACHUPB_000246"
     CDS             38573..40054
                     /gene="tolC"
                     /locus_tag="ACHUPB_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0757171.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40202..40873
                     /locus_tag="ACHUPB_000247"
     CDS             40202..40873
                     /locus_tag="ACHUPB_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0757172.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40879..42039
                     /locus_tag="ACHUPB_000248"
     CDS             40879..42039
                     /locus_tag="ACHUPB_000248"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0757173.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42077..42865)
                     /gene="ygiD"
                     /locus_tag="ACHUPB_000249"
     CDS             complement(42077..42865)
                     /gene="ygiD"
                     /locus_tag="ACHUPB_000249"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0757174.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43008..43781
                     /gene="zupT"
                     /locus_tag="ACHUPB_000250"
     CDS             43008..43781
                     /gene="zupT"
                     /locus_tag="ACHUPB_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0757175.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43839..44009)
                     /gene="yqiD"
                     /locus_tag="ACHUPB_000251"
     CDS             complement(43839..44009)
                     /gene="yqiD"
                     /locus_tag="ACHUPB_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0757176.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44270..44923)
                     /gene="ribB"
                     /locus_tag="ACHUPB_000252"
     CDS             complement(44270..44923)
                     /gene="ribB"
                     /locus_tag="ACHUPB_000252"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0757177.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45027..45175)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45297..45587
                     /gene="ubiK"
                     /locus_tag="ACHUPB_000253"
     CDS             45297..45587
                     /gene="ubiK"
                     /locus_tag="ACHUPB_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0757178.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11265 a        12242 c        11460 g        10668 t
ORIGIN      
        1 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
       61 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      121 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      181 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      241 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      301 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      361 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      421 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      481 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      541 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      601 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      661 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      721 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      781 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
      841 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
      901 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
      961 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1021 attttcggga tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt gtcggttgcg
     1081 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1141 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1201 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1261 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1321 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1381 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1441 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1501 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1561 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1621 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1681 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1741 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1801 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     1861 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     1921 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     1981 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2041 aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta ccactcagaa
     2101 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2161 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2221 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2281 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2341 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2401 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc cgcgtgtttg
     2461 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2521 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2581 tcggcggtgg ttttggtcac ttttaccatt acgcgccgat gaaaattgag tacgccatca
     2641 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2701 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2761 gcaatgtggt gttaggcggc gtgtatgatg ccgcagagtt tcttgatgcg ggcagttata
     2821 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     2881 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     2941 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3001 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3061 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3121 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3181 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3241 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt
     3301 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3361 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3421 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3481 cccagcacca agcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3541 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3601 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3661 ggataatttc aacggcgctc tggcaaagag acaactcatg cattttcgcc actcctgcga
     3721 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3781 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catgctgtgc
     3841 gaaagcggcg acatcagcga acaggagaga tattgcgaaa caccgtccag ttcgccaacg
     3901 gtaaaggtca tagtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     3961 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4021 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4081 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4141 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4201 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4261 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4321 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4381 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4441 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4501 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4561 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4621 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4681 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4741 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4801 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     4861 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     4921 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     4981 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5041 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5101 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5161 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5221 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5281 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5341 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5401 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5461 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5521 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5581 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5641 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5701 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5761 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     5821 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     5881 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     5941 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6001 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6061 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6121 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6181 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6241 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6301 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6361 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6421 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6481 caatagaaat caacagttct tcccaggtcg ggaagtaggc gtaaccgccg cccgggttga
     6541 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6601 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6661 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6721 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgatgaaa atcgccagca
     6781 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     6841 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     6901 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     6961 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7021 caagtgcaat gatgaagaac ataaccttgt tcagacgctt gagagaaacc ttccagccca
     7081 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7141 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7201 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7261 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7321 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtgccaatc agcaggtcaa
     7381 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7441 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7501 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7561 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7621 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7681 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7741 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7801 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     7861 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     7921 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     7981 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8041 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8101 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8161 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8221 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8281 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8341 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8401 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8461 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8521 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8581 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8641 cgcctgcaga gacgttgccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8701 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8761 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     8821 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     8881 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     8941 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cggtttttgt
     9001 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9061 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9121 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9181 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9241 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9301 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9361 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9421 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9481 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9541 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9601 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9661 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9721 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9781 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
     9841 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
     9901 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
     9961 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10021 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10081 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10141 tatatcgcca tatatagcga tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10201 catgcttgtt taagacgtaa tccagtaaga gataaaccta tcaggagaga tatttatcga
    10261 accatttcag tgtcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg
    10321 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcataa accttattat
    10381 tggctttcaa cgccgcctcg taagcaggcc agccctcgtt gattcgggtg tccagttccg
    10441 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10501 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacgc
    10561 caccgccata gcaaaatccg gtaataccca ctttgcctgc cgcttgtgga tagcgttgca
    10621 taaactcaat tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca
    10681 gctcacgacc tttatcatca tttcccggat aacctccaac ggaacttaag ccgtcaggtg
    10741 ccagggcgat ataccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca
    10801 gtccacgatt ctcatgcacc accaccacgg ctggcgtttt gccgctcatt tttgcgggtt
    10861 tcaccagata accccgtacc tcgccgtgac catttggcga aggatacatg atgtactcag
    10921 caacaatctc cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca
    10981 aatcaaacaa cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca aggttgagga
    11041 actcacgttt cgaaattttc ccgtgagcgt aatagtcgta gtaatccaac aactcttgtg
    11101 ggaaatcttt ggcggttaaa cgcggcatcg ttgcactcct cagttggtgt ttttttaagc
    11161 aaagcataag cacgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat
    11221 acgcgctggc gaatcgctaa actagaaaca ttgtttcgaa attgaacggt ggaaaggaga
    11281 ggtcatggtc tggttagcga atcccgaacg ttacgggcag atgcaatacc gctattgcgg
    11341 aaaaagcggt ttacgcctgc ccgcgttatc gctcggttta tggcacaatt tcggtcacgt
    11401 taacgcgctg gaatcacagc gtgcgatcct gcgtaaagcg tttgatttgg gcattacgca
    11461 ctttgattta gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg
    11521 cctgctgcgg gaggattttg ccgcttatcg cgatgaactg attatctcta ccaaggctgg
    11581 ctacgatatg tggcccggcc cttacggctc tggcggttca cgtaaatacc tgctcgccag
    11641 cctcgaccaa agcctgaagc gtatagggct tgagtatgtc gatatctttt actctcatcg
    11701 cgtcgatgaa aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag
    11761 cggtaaggcg ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc aaaaaatggt
    11821 cgagttgctg cacgagtgga aaattccgct tttaattcat caaccttcgt acaatttact
    11881 gaaccgctgg gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg
    11941 tattgccttt actcctctgg ctcagggatt gctgaccgga aaatatctca acggcattcc
    12001 gcaagattca cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct
    12061 taccgaagcc aacctcaaca gcctgcgctt attgaatgaa atggcacagc agcgtggaca
    12121 atcaatggcg caaatggcgt taagctggtt gctgaaagat gatcgcgtga cgtcggtatt
    12181 gattggtgcc agccgcgcgg agcaactaga ggagaacgtg caggcgctga ataatctgac
    12241 atttagcacc gaggagctgg cgcagattga tcagcatatc gccgatggcg agctgaatct
    12301 gtggcaggcg tcgtccgata aatgacctgt taataacggg cgcgcgaacc gcgcccgaat
    12361 cagatcagtg attatgacga gtcagtcggt caagatagcc catcacaaat gcagaaagca
    12421 caaacgtcag atggataatg acgtaccaca tcagtttgtt atccgggaca tttttcgcat
    12481 ccataaagac gcgcagtaag tgaatggaag aaattgccac aatcgacgct gctactttgt
    12541 ttttcagcga cgttgcgtcc attttcccca gccagttcag cttctctttg ttctcggaga
    12601 tatccagctg tgagacgaaa ttctcataac cggaaaacat caccatcacc agcaaaccgc
    12661 caaccagcgt catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg
    12721 agaagatatt cggcagtacg tgaataatct cctggaagaa cttcagcgcc agggcaacta
    12781 acgccagcga aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat
    12841 tttcaagaaa acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc
    12901 aattgagtaa acagcacgca acagcaaatc aacatatacg ttaaaccttt gtcgcataat
    12961 tctgaggttt agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa
    13021 aggacgttga acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt
    13081 attacactgg tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga
    13141 caccggtacc ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta
    13201 aagcactggt gacagggggc gattccggaa taggtcgcgc tgccgccatc gcttacgcgc
    13261 gtgaaggggc tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg
    13321 tgaaaaagat cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg
    13381 atgagaaatt cgcccgttcg ctggttcacg aagcgcacaa ggcgttaggc gggctggata
    13441 ttatggcgct ggtcgccggg aaacaggttg ccattccgga tattgcagac ctcaccagcg
    13501 aacagtttca aaagaccttt gccattaacg ttttcgcgct gttctggcta acccaggaag
    13561 cgatccccct gctaccgaaa ggtgcaagta tcatcaccac ttcgtcaatc caggcatacc
    13621 agccaagtcc gcatttactg gactatgcgg ctacgaaggc ggcgattctg aactacagcc
    13681 gtggcctggc aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc
    13741 ctatctggac ggcactgcaa atttccggcg gacaaacgca ggataagatc ccgcagtttg
    13801 gtcagaaaac gccgatgaag cgtgcggggc aaccggcgga actggcccct gtatatgttt
    13861 atctggcaag tcaggagtcg agctacgtca ccgcagaagt gcacggcgtg tgcggcggcg
    13921 agcatttagg ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat tgtgaattca
    13981 ctcttacctg atgtggcgaa aacgccttat ccggcctaca aacgcacgaa gattcaacat
    14041 gttgcaatag attttgtagg cctgatgagc gtagcgcatc aggcaattct actttacttc
    14101 gctttggcgg tttcttcgcc gaccagacct atcttcaggt aacccgcctg atgcagcgta
    14161 tccattacct tcatcaacgt ctcgtaatcg acggttttgt ccgctcggaa gaagatggtg
    14221 gtgtctttct tgccttcggt taacgcattc aacgccgtaa tcattgtttc atcggtgacc
    14281 ggatcgttac cgataaacat cgagttgtct gccttcaccg acagataaac cggtttttcc
    14341 gggcgcggct gcggcgtgct ggtagaagca ggcaagttca ccttcacatc taccgtcgct
    14401 aacggtgccg ccaccataaa gataatcagc agaaccaaca tcacgtcgat aaacggcgtc
    14461 acgttgatat catgcatttc gccgttatcg tcgaggtttt cgttaagatg cattgccatc
    14521 ggacattatc ctgcgcgtaa tttttgtgcg acacgaaccg gatgcgcagc ggcgctggct
    14581 tccagatcca ggtcacggct ttgcagcaac aatacctgcg ctgcaacatc acccagcatc
    14641 gctttaaagc cgccaatctg gcgtgcaaat acgttataga taacgaccgc aggaatcgct
    14701 gccacgaggc cgattgccgt tgctaacaga gcttctgcga tacccggcgc aacgactgcc
    14761 aggttagtgg tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc ccagaccgta
    14821 ccaaacagac caacgaacgg agaaatcgcg ccgatggttg ccagatagcc gttaccgcgg
    14881 cccatttgac gacccactgc ggcgacccga cgctccagac ggaagctggt acgttcttta
    14941 ataccttcgt tatcgtcgct gccttctgac agttccagct cattctgcgc ttcattgagc
    15001 aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga tatcgttggc ctggtttaag
    15061 gaacgcgctt cagccagcag ttgctgctcg cgcttaaggc gacgcttctg attgaagaac
    15121 tctacgctct tactgaagaa gattgcccaa gtgactacgg aggccaaaat aagcccaatc
    15181 atcacgcact taacgacaat atcggcgtgc tgatacatac cccagacgga aaggtccgtc
    15241 tgcatcaaat tattacccac tgtgtatctc caggacgcaa gtcacaaaat ctgcgcataa
    15301 taatatcaaa atgacgtcga attgatagtc gttctcatta ctatttgcat actgccgtac
    15361 ctttgctttc ttttccttgc gtttacgcag taaaaaagtc accagcacgc catttgcgaa
    15421 aattttctgc tttatgccaa ttcttcagga tgcgcccgcg aatattcatg ctagtttaga
    15481 catccagacg tataaaaaca ggaatcccga gatgacggac aaaaagcttg atactcaact
    15541 ggtgaatgca ggacgcagca aaaaatacac tctcggcgcg gtaaatagcg tgattcagcg
    15601 cgcttcttcg ctggtctttg actgtgtgga agccaaaaag cacgcgacgc gcaatcgcgc
    15661 caatggcgag ttgttctatg gacggcgcgg aacgttaacc catttctcct tacaacaagc
    15721 gatgtgtgaa ctggaaggtg gcgcaggctg cgtgctattt ccctgcgggg cggcagcggt
    15781 tgctaattcc attcttgctt ttgtcgaaca gggcgatcat gtgttgatga ccaacaccgc
    15841 ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc aaactgggcg taacgacatc
    15901 gtggtttgat ccgctgattg gtgccgatat cgttaagcat ctgcagccaa acactaaaat
    15961 cgtgtttctg gaatcgccag gctccatcac catggaagtc cacgacgttc cggcgattgt
    16021 tgccgccgta cgcagtgtgg tgccggatgc catcattatg atcgacaaca cctgggcagc
    16081 cggtgtgctg tttaaggcgc tggactttgg catcgatgtt tctattcaag ccgccaccaa
    16141 atatctggtt gggcattcag atgcgatgat tggcactgcc gtatgcaatg cccgttgctg
    16201 ggagcagcta cgggaaaacg cctatctgat gggccagatg gtcgatgccg ataccgccta
    16261 tataaccagc cgtggcctgc gcacattagg tgtgcgtttg cgtcaacatc atgaaagcag
    16321 tctgaaagtg gctgaatggc tggctgaaca tccgcaagta gcgcgagtta accaccctgc
    16381 tctgcctggc agtaaaggcc acgaattctg gaaacgagac tttacaggca gcagcgggct
    16441 attttccttt gtgcttaaga aaaaactcag taatgaagag ctggcgaact atctggataa
    16501 cttcagttta ttcagcatgg cctactcgtg gggcgggtat gaatcgttga tcctggcgaa
    16561 tcaaccagaa catatcgccg ccattcgccc acaaggcgag atcgatttta gcgggacctt
    16621 gattcgcctg catattggtc tggaagatgt cgacgatctg attgccgatc tggacgccgg
    16681 ttttgcgcga attgtataac attgccactt ttggacaatt ttgcagacat ttaattgtga
    16741 aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg acgaatcagg agtacaatag
    16801 gcagataaag gcttaaacgc tgttccacag gaaagtccat ggctgttatt caagatatca
    16861 tcgctgcgct ctggcaacac gactttgccg cgctggcgga tcctcatatt gttagcgttg
    16921 tttactttgt catgtttgcc acgctgtttt tagaaaacgg cctgctgccc gcctcatttt
    16981 tgccaggcga cagcttgttg atactggcag gcgcattgat tgcccaaggg gttatggatt
    17041 ttctgcctac gattgcgatt ctgaccgccg cagcaagtct gggctgctgg ctaagttata
    17101 ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg ctggctggca cagcttcctg
    17161 ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca cggtctgctg gcgctgctgg
    17221 ctggacgttt tcttgcattt gtccgtacgc tgctgccaac catggcggga atttccggtc
    17281 tgccaaaccg ccgcttccag tttttcaact ggttaagtgg attgctgtgg gtcagcgtgg
    17341 taaccagttt tggctatgcc ttaagtatga ttccgttcgt taaacgccat gaagatcagg
    17401 taatgacgtt cctgatgatc ctgccaattg ccttgttaac cgctggcttg ttaggcacgc
    17461 tgtttgtagt gattaaaaaa aaatactgta acgcctgacg attttccccg ttcccggctg
    17521 ctgtaccggg aacgtattta attcccctgc atcgcccgca ttcttgccgc atcttccccc
    17581 ggcgtcacac cgaagtaacg tttaaactca cggctaaatt gcgatgcgct ttcatagtcg
    17641 acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat ccgcgcctta
    17701 tgcagacggt aattcttcaa atactgcaac ggcgaggtgc tggtgacaga cttaaaatta
    17761 tgatggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac gctcaggttt
    17821 tcggtgtatt tattctcaat ccgtttcagc acgcggctaa tcagactgaa gtgagtctgg
    17881 cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta gtacagaatt
    17941 tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat cacgtcgagt
    18001 aaccgctccg ccgcgcataa aatctcttct gataacgtgg cggagttaat cccgctggct
    18061 gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc ctgtaactgc
    18121 aaaatatcga cattgagacg caatcctgcc agcggcacct ctgacgtggc ataggtttcg
    18181 cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata acgaaacacg
    18241 cgttcattga tataaccgat tttatgcccg gaaaagagaa ttatgatgcc aggctcgtac
    18301 atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca aacgcacatc gggcaacagt
    18361 tctgacaaac tattttcttt atttttcagt ttattaactt tatccgccag caagcggcaa
    18421 atctcttcac gtttcatatc gcgtaatttc ttaggaataa tgcggcaatt tgattgtgcg
    18481 caattttgta gcatttctcc agcactctgg agaaataggc aagacattgg cagaaatgag
    18541 cattgagagc cagggcgctg gcgatcacaa tgaaaaacat caggcagatc gttctctgcc
    18601 ctcatattgg cccagcaaag ggagcaagta atgaacaact ttaatctgca caccccaacc
    18661 cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg aacaaattcc tcacgatgct
    18721 cgcgtattga ttacctacgg cggcggcagc gtgaaaaaaa tcggcgttct cgatcaagtt
    18781 ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg gtattgagcc aaacccggct
    18841 tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac agaaagtgac tttcctgctg
    18901 gcggttggcg gcggttctgt actggacggc accaaattta tcgccgcagc ggctaactat
    18961 ccggaaaata tcgatccgtg gcacattctg caaacgggcg gtaaagagat taaaagcgcc
    19021 atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt cagaatccaa cgcaggcgcg
    19081 gtgatctccc gtaaaaccac aggcgacaag caggcgttcc attctgccca tgttcagccg
    19141 gtatttgccg tgctcgatcc ggtttatacc tacaccctgc cgccgcgtca ggtggctaac
    19201 gacgtagtgg acgcctttgt acacaccgtg gaacagtatg ttaccaaacc ggttgatgcc
    19261 aaaattcagg accgtttcgc agaaggcatt ttgctgacgc taatcgaaga tggtccgaaa
    19321 gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg tcatgtgggc ggcgactcag
    19381 gcgctgaacg gtttgattgg cgctggcgta ccgcaggact gggcaacgca tatgctgggc
    19441 cacgaactga ctgcgatgca cggtctggat cacgcgcaaa cactggctat cgtcctgcct
    19501 gcactgtgga atgaaaaacg cgagaccaag cgcgctaagc tgctgcaata tgctgaacgc
    19561 gtctggaaca tcactgaagg ttccgacgat gagcgtattg acgccgcgat tgccgcaacc
    19621 cgcaatttct ttgagcaatt aggcgtgccg acccacctct ccgactacgg tctggacggc
    19681 agctccatcc cggctttgct gaaaaaactg gaagagcacg gcatgaccca actgggcgaa
    19741 aatcatgaca ttacgttgga tgtcagccgc cgtatatacg aagccgcccg ctaagctttt
    19801 tacgcctcaa actttcgttt tcgggcattt cgtccagact taagttcaca acacctcacc
    19861 ggagcctgct ccggtgagtt catataaagg aggaacgtat ggctaatcca accgttatta
    19921 agctacagga tggcaatgtc atgccccagc tgggactggg cgtctggcaa gcaagtaatg
    19981 aggaagtaat caccgccatt caaaaagcgt tagaagtggg ttatcgctcg attgataccg
    20041 ccgcggccta caagaacgaa gaaggtgtcg gcaaagccct gaaaaatgcc tcagtcaaca
    20101 gagaagaact gttcatcacc actaagctgt ggaacgacga ccacaagcgc ccccgcgaag
    20161 ccctgctcga cagcctgaaa aaactccagc ttgattatat cgacctctac ttaatgcact
    20221 ggcccgttcc cgctatcgac cattatgtcg aagcatggaa aggcatgatc gaattgcaaa
    20281 aagagggatt aatcaaaagc atcggcgtgt gcaacttcca gatccatcac ctgcaacgcc
    20341 tgattgatga aactggcgtg acgcctgtga taaaccagat cgaacttcat ccgctgatgc
    20401 aacaacgcca gctacacgcc tggaacgcga cacacaaaat ccagaccgaa tcctggagcc
    20461 cattagcgca aggagggaaa ggcgttttcg atcagaaagt cattcgcgat ctggcagata
    20521 aatacggcaa agccccggcg cagattgtta tccgctggca tctggatagc ggcctggtgg
    20581 tgatcccgaa atcggtcaca ccttcacgta ttgccgaaaa ctttgatgtc tgggatttcc
    20641 gtctcgacaa agacgaactc ggcgaaattg caaaactcga tcagggcaag cgtctcggtc
    20701 ccgatcctga ccagttcggc ggctaacatg caaattctcc cggtgacggt aatgttccgc
    20761 taccggactt ttcagaaatc atttattccc ctcgcgtccc gcccgttgtt actcttcctt
    20821 gttcaggaat gccaaatata aggacatcat catgcagagc cggaagctct taaaagaaca
    20881 actcatctat atccgggata aacgcaacgg agaggtgaaa aacagatgaa aataatactt
    20941 ctgtttttag cagccctggc aagttttacc gtacacgcgc agcctccttc acagaccgta
    21001 gaacaaacag tccggcagat ttatcagaac tataaatcag atgccagtac accttatttc
    21061 ggtgaaaccg gagagcgggc gataacttca gcgcgtattc aacaggcgct taccctgaac
    21121 gacaatctta cgctgccggg caatattggc tggctggatt atgatccggt ttgtgattgt
    21181 caggattttg gcgatctggt gctagaaagc gttgcgataa cccaaactga cgccgatcat
    21241 gccgatgccg ttgtgcgctt tcgtatcttt aaagatgata aagaaaagac cacgcagaca
    21301 ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgacg atattgtcag caatcatggc
    21361 agcgtcttac aagcagttaa tagcgagaat gaaaaaacgc tggccgcttt agcttcgttg
    21421 caaaaagaac agccggaagc ctttgttgcc gaactctttg aacatattgc tgattatagc
    21481 tggccgtgga cgtgggtggt ttccgactct taccgccagg cggttaatgc cttctataaa
    21541 accaccttca agacggccaa taatcccgat gaagatatgc aaatagaacg gcaatttatt
    21601 tacgacaatc cgatctgttt tggcgaagag tcgctatttt cacgcgttga tgaaattcga
    21661 gtcctggaga aaaccgccga ttccgcccgc attcatgttc gttttacgct gaccaatggc
    21721 aacaacgaag agcaagaact ggttttacag cggcgcgaag gcaagtggga aatcgctgat
    21781 tttatccgcc cgaacagcgg cagcctactt aagcagattg aggcaaaaac cgccgccaga
    21841 ttaaagcaat gagctgaatt aaataacaat tagccggaac aataaataaa agggaacact
    21901 atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcttg ctcagtggct
    21961 gcgtcaatga gcaaaaggtc aatcaactgg cgagcaatgt acaaacatta aatgccaaaa
    22021 tcgcccggct tgagcaggat ataaaagcac tacgcccaca aatctatgct gccaaatccg
    22081 aagctaacag agccaatacg cgtcttgatg ctcaggacta ttttgattgc ctgcgctgct
    22141 tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc cggggatttt
    22201 ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac cagcattcac cggacgagat
    22261 tgcgtagacg acgctttttt tgccgtagca ggcgtctgac gctgggtcgc catcggcgta
    22321 tgtttagtca acgccggacg ggtattgcgg ttctggcgac gagcttcacg catctcttca
    22381 atggttggcg caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc
    22441 gcttccagcg ccgggcggat taacggccag tttgccggat cgtggtaacg caacaacgct
    22501 ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga cgtcttcgct cttataacca
    22561 atcttcgcca gcggtttttt cccggtgtaa tacatggtgg ttgagttcgc cagcggcgat
    22621 gggtagaagt tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaaa
    22681 ttcaccatat cttcatcacg cgtaccgggg tgcgcagaga tgaaatacgg gatcagatac
    22741 tgctctttac ctgcctgttt cgagtaggta tcgaacagct ctttaaagcg gtcatagctg
    22801 cccatgcccg gcttcatcat cttcgacaac ggcccttctt cggtatgttc cggagcaatc
    22861 ttcagataac cgccgacgtg atgggtcgcc aactctttga tatagcgcgg atcttccacg
    22921 gcgatgtcat aacgtacgcc tgaggcgatg aggatttttt taatgccttt cagatcacgc
    22981 gcacggcgat agaggttgat cgtcggttcg tggttagtgt ccatgtgcgg acaaatatcc
    23041 ggataaacgc acgacaaacg gcgacaagtt tgttcagcgc gtggcgattt gcagtgcaac
    23101 atatacatgt tggcagttgg cccaccgaga tcggaaatca cgcccgtaaa acctggaacg
    23161 gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt ccgaacggct ctgaataatg
    23221 cgcccttcgt gctcggtaat agaacagaaa gaacagccgc caaagcagcc gcgcataatg
    23281 ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg cattgccata ggccggatgt
    23341 ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct cttcggtaga aagcgggata
    23401 gcaggcgggt tgatccacac atagcggtca ccgtgttttt gcatcaatgc gcggacgcag
    23461 cctgggttgg tttcgtggtg cagaatacgc gaagcatggg cgtacagcac tttatcgccc
    23521 ttcactttct cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt
    23581 ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg
    23641 cacggcaaat cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga
    23701 cggggggaat ccacgccgct ccagccaggc agcgcctctt tcacgataat cgcggtatta
    23761 cgcacatcgc ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc
    23821 agcggacgct caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa
    23881 cggcgcacgg tatcggacca gtaatcataa tgtgcggtac ggcgcagact agcctcaata
    23941 ccgccgagga tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt
    24001 gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt
    24061 aaacggcgat cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg
    24121 aaaaacagat tcggtttacc cagacgcata aagtcgtctt tgctgctcca gtccggctgg
    24181 gcgatgatcc cgacgcgaaa gccctgtgct tccagcatac gaccgcaaat cgccatcccg
    24241 aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag
    24301 ccaagttgat ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg
    24361 gcccagtact gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc
    24421 attttgcttc cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga
    24481 aagagaaatc gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta
    24541 ccggattgac taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
    24601 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
    24661 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcaccat
    24721 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
    24781 gtaccgtcca gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc
    24841 acagctgtcc attaataccc gggtcatcac ccagactgat atcgcgactg cgaatcggcg
    24901 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
    24961 gaaggccggt tgggcgcagc gttagcacca gggtagaaac cagaatactg gatggctcaa
    25021 agaagccgcg aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat
    25081 cgccgttgct catatccacc agaatctcgc ggcgctcgcc cggtgccagc gaaagttgct
    25141 tcaccgatac aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
    25201 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca
    25261 cccagccacg cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac
    25321 caacaaagcc gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt
    25381 tatcctggat aatgaccgga aaatcatcca catcataatg gttggggata ggcagcgact
    25441 tgctgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
    25501 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga ataggcagta
    25561 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggccag
    25621 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
    25681 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
    25741 cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
    25801 acagcggttg cccacggcga gattcaagta gcggcggaac gggtagcggt tgctgttgcc
    25861 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
    25921 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
    25981 tgcaggaaag tgttccccgc aaaaatacag attgccgact taaacttttc cggcggcttc
    26041 gcgttctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
    26101 ggcagccagc tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
    26161 ttcgacaatc accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtaga
    26221 gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ctccagtctt
    26281 gaacggtagc aggccgcgac cacggctgcg ggttccttcc gggaacatcc agatggaaat
    26341 acggcgtttt ttgaaatgat tcacgacttc cgcaatggtg ccgtgagctt tagtgcgatt
    26401 gtttctgtcg atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca
    26461 cagcaagctc tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac
    26521 catgtcatag ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc
    26581 tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc gcaagacggc caaacatatg
    26641 cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa
    26701 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
    26761 accctctgaa ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg
    26821 ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt
    26881 tcaagggaat ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt
    26941 tcatccggcg ttccttgcaa gcgggaggaa acaggccctc cccggcatat tactcttcgc
    27001 tatcaccgtt gctggcacgg cgaggagagt cgatctcaac acgatcgata cgctgcaaac
    27061 cgcgcatcaa cgtaccgcgg cgtccacgtt caccagtgac tttctgtaac tcttccgggc
    27121 gcagtttaat tttgcgtttc ccaacatgaa tggtcagcgt gctttgcggc ggcagaacgt
    27181 acaattgcgc cagaccatct tctccacgcg cggcttctgc cgatggaatg ttgataatct
    27241 tgttgccttt gcccttcgac agctgcggca gatcgcttac cgggaacatc aacatacggc
    27301 ctgcctgagt gattgccagc agcatatcgg aagcatcttc aatcaccacc ggcggcataa
    27361 catgggcatt ttccggtaag gtgatcaaag ccttacctgc acggttacgc gccaccagat
    27421 cgttaaaggt gcagacgaaa ccgtaacccg catcggaagc catcagcagt ttctgatcgt
    27481 cgctttccat cagcatatgg tcaacggtcg ccccaggcgg caacgttaat ttgccggtga
    27541 gtggttcgcc ctgaccacgc gccgacggca gcgtaatcgg gtcgatggca tagctacgac
    27601 cggtggaatc aacaaacact accgattggt tgctcttacc tttcaccgcc actttgaagc
    27661 tatcacccgc tttataattc aggcccggcg cgtcgatatc atggccttta gcgctgcgta
    27721 cccagcccat ctgcgacagc acaatggtga caggttcaga cggcagcatg tcgtgctcgc
    27781 tcatcgcttt cgcttcttcg cgttcctgca acggcgaacg acgatcgtca ccgtaggctt
    27841 gcgcgtctgc ctgcagttct ttcttcagca ggttattcat tttacgctcg gaagccaaaa
    27901 tgccctgcaa ctggtcgcgc tctttttcca gctcactctg ctcaccgcga atcttcatct
    27961 cttccagttt ggcaagatga cgcagtttca gttcgaggat cgcttccgcc tgggtttccg
    28021 taaggccaaa ccgcgacatc agcgccggtt tcggttcatc ttcgttacga atgatctcaa
    28081 tcacttcgtc gatgttgaga aacgccacca gcaaaccttc caggatatgc aggcgcttga
    28141 ggactttctc cagacgatag ttcagtcggc ggcgcacggt atcgcgacgg aacaccagcc
    28201 attcggagag gatttccagc aggtttttca ccgccggacg accatccaga ccgatcatat
    28261 tgaggttaat gcgatagctc ttttccagat cggtggtagc gaagaggtgg ttcatcacct
    28321 gatccatatc cacgcggttg gaacgcggca caatcaccag acgggtcggg ttctcgtggt
    28381 cagattcatc gcgcagatcg tcaaccatcg gcagcttttt gttgcgcatt tgcgcagcaa
    28441 tttgctccag tacacgcgca cctgaaacct gatgcggtaa tgcgctgata accaccgcgc
    28501 catcttcttt cttccacacc gcgcgcatac gtactgaacc acgtccgttc tcgtagattt
    28561 tacggatctc ggcgcgcgaa gtgataattt ccgcttcagt cggataatcc ggcccctgca
    28621 cgatatccag cagctgatcg agcgtggttt tcggctggtc gattaatgcg attgccgcct
    28681 gagctacttc acgcaggtta tgcggtggaa tatcggtcgc catgccgacg gcaataccgg
    28741 tggtgccgtt aagcaaaatg tttggcagac gagcaggcag cattttcggc tcctgcaaag
    28801 tgccgtcgaa gtttggcacc cagtcagccg tcccctgccc cagctcgctc aatagcagct
    28861 cggaatattt cgacaaccgg gattcggtgt aacgcattgc cgcgaacgat ttcggatcgt
    28921 ccggcgcgcc ccagttcccc tgaccatcaa ccagcggata acggtaagag aacggctgcg
    28981 ccatcaggac catcgcttca taacaggcga tatcgccgtg cggatggtat ttacccagta
    29041 cgtcaccgac ggtacgggcc gattttttaa atttggcgct ggcattcagg cccagttcag
    29101 acatcgcata cacaatgcgg cgctgaacag gtttcagacc atcaccaata aacggcaacg
    29161 cacggtccat gatcacgtac atggagtagt ttaagtaggc gttttccgta aattcatgta
    29221 gcgcaaggcg ctctgccata tcgctcatta attctgattc ctcaactgat tcgccagcct
    29281 tcaaaggggc aatattgccg gagatactac attatctgcc gcgtcgagtc acaggagatc
    29341 agcgtgaata gcgccgccgg acgtgctccg acggcgataa aggcatcaat gtgccttgat
    29401 atacaacgct ttgctgtaga cttgatcggc atagagggca gcacaaggtt gcgcccatgc
    29461 cacaccactc ggcatggggg tccgctccgg gcgattcgag aagttgaggt tatgcgagtc
    29521 cagggaagcc taaagcatca aagtagtcga ctgtaagtat atggttcagc ccagtttagc
    29581 tgtgtctcca ccagtgcccc gcgcctcctg ccataagtgc ggcttgctca tgggtacccc
    29641 cgagacggct tacggcgcac cggataataa atttcgcgta tacatggtat tccttaccgg
    29701 agaaattata agcccaacaa caatacgcct gtggcattgt tcgctcaaac ttatcgcgag
    29761 tgatttggct cacactccgg taaagattcc ccatcacgct aacggcacat aaacatccgt
    29821 ttccagtcgc tgctccgtca ccccttcggc aagattggtg tagtgaaaca gaatcggtgc
    29881 tttacgcatt ttctcgccgc ttgcaggcag ccagtggcga ataatgcccc atatcgtgtg
    29941 tgaaatatcg tccagctcgc caacgtggcg ggccacggca taacgtccac cggtaagttc
    30001 accattgctc acaccataac gattatcggg aatcggttcg ctaacgctgc cgcagatatc
    30061 aaagcgaaac gcttccggtg cggtggtggc agggtcgtcc caggcgacgc caaaagtctg
    30121 gctttggtta acaggagaaa gccccgtctc tttacgccac atgataaatt tcgccacact
    30181 gtagttgagc aactcagggc tacagcggtg cggcaacatc gccacaggga ttgatgggaa
    30241 atcgataatg tttacatcca gtgtcaggtt tgtcattttg cctccctgcg aaattattcc
    30301 cgggaacgcg tcccgggagc ggtaacaatt acggatttac tttgcggatc tgtttgacgt
    30361 caatttcaac agaattccag tctttatcga cttcaccctg aatctcaacc gtatctttcg
    30421 gcgtcaccgt cacaccgttc cagcgtttgt ggtcgatatc aacattgata gtaccgctgg
    30481 catctttaaa cacgtagaga tcgtcagaga tgcgttcaac gatattgccg cgcagggtta
    30541 cccaggtgtc gtcacgcagg gattttgcgc tttctacagt cgttacgctg ccgttcggcc
    30601 cctggaatcc tccggcctga ctttgcgttg ccgatgggcc agaaaaaccg ccctgctctg
    30661 ctgccatcac cggtgcactg cacagggcca ttactgcgat tactgctgcg aattttttca
    30721 tgtttattac tccctttaat gtctgtttcc gagcatttaa caagatagtc cttaacaact
    30781 tcttaaggga aaaaaataaa atttaatgct gtacagagcg cgttacaaca cggtttactg
    30841 gcagcaaata cggttatcgc agggatgaaa aaatgcgaat tttactgata gaagatgaca
    30901 tgctgattgg cgacggcatc aaaactggcc ttagtaaaat gggttttagt gtcgactggt
    30961 ttacacaagg tcgtcaggga aaagaggcgc tttatagcgc accttatgat gcggtgatcc
    31021 tggatttaac cttaccaggc atggatggtc gcgatatttt gcgcgaatgg cgagagaaag
    31081 gtcagcgtga gccggtactg atcctgaccg cgcgtgatgc gttagcggaa cgtgtagaag
    31141 ggctgcgtct gggagctgac gattatctgt gtaaaccttt tgcgttgata gaagtcgccg
    31201 ccaggctgga ggctctgatg cgccgaacca acggccaggc cagcaacgag ctgcgccacg
    31261 gtaacgtcat gctcgacccc ggcaaacgta tcgccacgct ggctggcgaa cccttaacac
    31321 tgaaaccaaa agaatttgcc ctgctggaat tactgatgcg taacgctggt cgggtactgc
    31381 cgcgcaaact gattgaagag aaactgtata cctgggacga agaggtcacc agtaatgccg
    31441 ttgaagtgca tgtgcatcat ctgcgacgca aactcggtag tgattttatt cgtaccgtgc
    31501 atggtattgg ctacacatta ggtgagaaat gaaatttacc caacgtctta gtctgcgcgt
    31561 caggctgacg ctaatctttt taattctggc ctcggtgacc tggctgcttt ccagctttgt
    31621 cgcctggaaa caaacaacgg ataacgtcga tgaattgttc gacacccaac tgatgctgtt
    31681 tgccaagcgg ttaagtacgc tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca
    31741 gacgccaaat aaattaaaac acggtcatgt tgatgacgat gcgctgacct ttgccatctt
    31801 tacccacgac ggcagaatgg tccttaatga tggcgataac ggagaagata ttccctatag
    31861 ctatcaacgg gaaggttttg ctgacgggca actggtcggt gaagacgatc cttggcgttt
    31921 tgtctggatg acctcacctg atggcaaata tcgcatcgtt gttggccagg aatgggaata
    31981 ccgtgaagac atggcgctgg cgattgttgc cgggcaattg atcccgtggc tggtcgcact
    32041 gccgattatg ttaatcatca tgatggtact actgggtcgt gaactcgcgc cgctgaacaa
    32101 actggcgctg gcactacgta tgcgtgaccc tgactcggaa aaaccactaa acgcgactgg
    32161 cgtacccagc gaagtgcgac cactggttga gtcgctaaat caactgttcg cccgcacaca
    32221 tgcgatgatg gttcgagaac gacgctttac ctccgatgca gctcacgaac tacgtagccc
    32281 gttaacagcg ctgaaagtgc aaaccgaagt tgcgcagctc tctgacgatg atccgcaggc
    32341 gcggaaaaaa gcactgctcc aattacattc cgggatcgat cgcgctactc gtctggttga
    32401 tcaactgctc acgctatcgc ggctggactc actggataac cttcaggacg tcgcggagat
    32461 cccgcttgaa gatctcctgc aatcgtcggt gatggatatt taccacacgg cgcagcaggc
    32521 gaaaattgac gtgcgactga cactcaatgc ccacagcatc aaacgcactg gacaaccgct
    32581 attgctaagt ttgttggtgc gaaaccttct ggataacgca gtgcgctaca gtccacaggg
    32641 cagcgtggtg gacgtcacgc tgaatgccga taatttcatc gtgagggata acggccccgg
    32701 tgtgacacca gaggcactgg cgcgaattgg cgaacgcttc tatcgccccc ccggacaaac
    32761 cgctaccggc agtggacttg ggctatcgat tgtccagcga atcgccaaat tgcatggcat
    32821 gaatgttgaa tttgggaatg cggaacaagg tggatttgag gcgaaggtga gctggtaagg
    32881 gtaagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttt accgtaccgc
    32941 tctgtgacgt actatagtcg acaaacgtct caccttgagg ttaaaaaatg agcaacaccc
    33001 tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac gacaccctga
    33061 ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgttcgcatc gttcgcgccg
    33121 acagcgacta cgatgtcaaa gcggaagtac aaaactttct ctgggctgat gtggtgatct
    33181 ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatac attgatgatg
    33241 tattcaccga aggtcacggg acgctgtatg ccagcgatgg ccgtacccgc aaagatccgt
    33301 caaaaaaata cggttccggc ggcctggtac agggcaaaaa atatatgctt tctctgacct
    33361 ggaacgcacc aatggaagcc ttcaccgaaa aagatcaatt cttccacggc gttggcgtcg
    33421 acggtgtgta tctgccgttc cataaagcaa accaattcct tggcatggaa ccgctgccga
    33481 catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc
    33541 gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca
    33601 tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat
    33661 tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg
    33721 cgccaatggt ggattgcgct gctggcgtga gtttccagtc tatggcaccg gattctatcg
    33781 tgatgattga gcagtgggaa agcatcgcgc accttgaagc gcatctgcaa accccgcaca
    33841 tgaaggcgta cagcgaagcc gtaaaaggcg acgtgctgga gatgaatatt cgtattctgc
    33901 agccagggat ttaatcctgc cttgtttgcc cggccatcct gaccgggcaa tgttctttcc
    33961 tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg
    34021 agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat
    34081 cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag cgtggtttca cgcaattgca
    34141 tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt
    34201 ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc
    34261 cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg
    34321 cgcggaagtg ttttacgaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcagagt
    34381 ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg tcgctgttag
    34441 gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc acttcgtcgg
    34501 aagaaacttc ccaggtgtta aggatcttac ctttcagcgg catgatcgcc tgatattcac
    34561 gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccacgagg aacagttcgg
    34621 tacggttaag gtcctgcgcg gtacaatcag ccagtttgcc aggcagcgcc gggccgctgg
    34681 tcagcttttt acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg
    34741 ccatctctgc cagcaactcc gccgcctgaa cgttctggtt cagccacagg ataaaggcat
    34801 ctttcaccac gccagaaacg aatgccgcgc attgacgcga agagagacgc tctttcgtct
    34861 gcccggcaaa ctgcggatcc tgcattttca ccgacagaac ataggcgcag cgatcccaga
    34921 tatcttccgc cgacagcttc acgccgcgcg gcagaatgtt gcgatattca cagaactcac
    34981 gcatcgcgtc caacaggccc tgacgcaggc cattaacatg ggtaccgccc tgcatcgttg
    35041 ggatcaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg
    35101 cccagtccac agcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg
    35161 tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc
    35221 gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccgggg cacagtaccg
    35281 ctttggcttt cagcacatgc gtcaggcgtg aaacagaaaa tcgcgggctg tcaaagaagg
    35341 tttcatccgg ccagaagtgc acgctggtac cggtattgcg tttaccgcaa gtgccgacaa
    35401 cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc tgaccatcgc
    35461 ggcgcacgtt aacttcaacg cgcttcgaca gggcgttaac caccgaaatc cccacgccat
    35521 gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc
    35581 aaagaatcaa ttcaaccgcc gggacaccct cttccgggtg aatatccacc ggcatcccgc
    35641 gcccatcgtc aataacttct aacgactggt cagcatgtaa gattacatcc acgcgttttg
    35701 cgtgacccgc cagtgcttca tcgacactgt tatcaataac ttcttgcccc aaatggttag
    35761 ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta
    35821 cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat
    35881 cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc
    35941 gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc
    36001 gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc
    36061 gcttccagcg ggtcaatctg catgactttg agatcgtaaa tatggcgtga ctctagcaca
    36121 tattgctgcc cggtgtatgg gttctcgttc tgaccgagat agtccgtcag cagttcaaac
    36181 gggcgcaccg ccgggtttac caccactgcg ggcagcataa aacattgtga caaccaggtg
    36241 gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg
    36301 acaatggatt ccagcagttc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc
    36361 atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag
    36421 cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag
    36481 cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat
    36541 cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta aagttggaac
    36601 agtgcggctt aaactgcaca caggtcgacg gcgttgccag caggcggcga ccgttccagt
    36661 cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg
    36721 ccagcacggt atccagttcg cccgcgttac gcagactgtg ttgatcgagc caactacaac
    36781 ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca
    36841 gtttacgttc cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca
    36901 cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacccgc ttcgccgggg
    36961 agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca
    37021 gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag
    37081 aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctgatgtg
    37141 gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagagctt
    37201 cgtgcttttg tgcaaacagg tgagtgtcgg taatttgtaa aatcctgact ctggcctcac
    37261 cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca cgatgctagt
    37321 aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact
    37381 gattaatttg atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg
    37441 ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca
    37501 gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg
    37561 tcaccagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca
    37621 cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg
    37681 aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt
    37741 atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg
    37801 acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca
    37861 catgaacgcg aatatcttcg ttttcatcag ccagaccatg aataccgctt gcggtcgtgg
    37921 cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga
    37981 aacttaacac cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac
    38041 gggcaacatc ttccacactt tcaccctctt ctatcatccc ggcaaccatc tccagtagcc
    38101 agggggtttc actggtgtcg tatgcggcaa tccgaatctg ctcaatcagc acaacttcat
    38161 cgcgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc
    38221 gtacctcatg actcatttgc ccgttgaata gacgatgacg aaatctataa agatctaatg
    38281 aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg
    38341 taacgggcag gttgtctggc ttaagcattg ttaatgtcct ggcactaata gtgaattaaa
    38401 tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tgtgttaaat tgaggcacat
    38461 taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca
    38521 ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa
    38581 attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga
    38641 gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc
    38701 cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca
    38761 gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa
    38821 ctctaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg
    38881 gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacat atcagaccga
    38941 tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga
    39001 cgttctttcc tatacacagg cacaaaaaga agcgatctac cgtcaattag atcaaaccac
    39061 ccaacgtttt aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcgcagta
    39121 cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca
    39181 gctgcgccag atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt
    39241 taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct
    39301 gtcgctgtta caggcacgct tgagccagga cctggcgcgc gagcaaattc gccaggcgca
    39361 ggatggtcac ttaccgacgc tggatttaac ggcttctacc gggatttctg acacctctta
    39421 tagcggttcg aaaacccgtg gtgccgctgg tacccagtat gacgacagca atatgggcca
    39481 gaacaaagtg ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca
    39541 ggtgaaacag gcccagtaca actttgttgg tgccagcgag caactggaaa gcgcgcatcg
    39601 tagcgtcgtg caaaccgtac gttcctcctt caacaacatt aatgcatcta tcagtagcat
    39661 taacgcctac aaacaagccg tagtttccgc tcaaagctca ttagacgcga tggaagcggg
    39721 ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcaaccacca cgctgtacaa
    39781 cgctaagcaa gagctggcaa atgcgcgtta taactacctg attaaccagt tgaatattaa
    39841 gtcagctctg ggtacgttga acgagcagga tctgctggca ctgaacaatg cgctgagcaa
    39901 accggtttcc actaatccgg aaaacgttgc accgcaaacg ccggaacaga atgctattgc
    39961 tgatggttat gcgcctgata gcccggcacc agtcgttcag caaacatccg cacgcactac
    40021 caccagtaac ggtcataacc ctttccgtaa ctgacgacga cgacggggct tcggccccgt
    40081 ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt
    40141 caatttcgac cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa
    40201 aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca
    40261 tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag
    40321 tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa
    40381 aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc
    40441 gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gccagcaggc
    40501 accagcccag gctggcatgg caccagaaaa ccaggcgcag gcccagcaat ccagcgggag
    40561 tttctggatg ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg
    40621 atttgcacag cagccgctgt tctcctcgaa aaacccagcc agcccggctt acggtaaata
    40681 taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc
    40741 gaagacagcc atggcaccaa aaccggcgac cactactacc gttacccgtg gcggttttgg
    40801 tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca accggcacct cttctcgttc
    40861 aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgcgaa
    40921 aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa
    40981 gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa
    41041 ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc
    41101 aaattccgca ttccaaaacg cacctggagt tttgtgcgtc agtcatggct gactcaccag
    41161 ccatcgcttt attcgcgtct tgatctggca tgggatggca ctggcgaacc taaactgctg
    41221 gaaaataacg ccgatacacc aacgtcacta tacgaggcgg cgttctttca gtggatctgg
    41281 ctggaagatc agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtctg
    41341 caagaaaagc taatcgatcg ctttgtcgag ttgcgtgaac agtatggctt ccagttgctg
    41401 catctcacct gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac
    41461 tgcgcgacgg aagctgaaat cgctaccgag ttcctctaca ttgacgatat cgggttaggt
    41521 gaaaaaggtc agttcacgga tttacaggat caggtaattt ccaacctgtt caagctgtat
    41581 ccatgggaat ttatgttgcg tgagatgttc tccaccaagc tggaggatgc aggcgtacgc
    41641 tggctggaac cggcgtggaa gagcattatc tccaacaaag cgcttctacc gctactatgg
    41701 gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg
    41761 caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc
    41821 attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt
    41881 gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg
    41941 ctggtgaacg atcaacctgc cggaattggc attcgtgaag accgtgcgtt gatcacccag
    42001 gatatgtctc ggttttatcc acatattttt attgagtaat caatgatgcc ggatggcatt
    42061 cgccatccgg taattgttaa cctatctgca ccgacagcat actcaggctg cccatttcta
    42121 taccatcgac cggaatggta attggctcct gcccatccca cgcacctaag acatacaaca
    42181 acggcagata gtgctccggc gttgggttcg ataacgcgcc accttcatgg tcaaggtaat
    42241 tcaccagagg atgctgctct accgcccctt gccacgtcag attcgctttc acatactcat
    42301 taaacgacat cgcccaagga tatggtgaac tatcaccgtg ccacttcact gtacgcaggt
    42361 tatgcaccac gttgccactg gcgaccaaca ttattccttc atctcgcagc gctgccagtt
    42421 tgcgccccat ttcgaaatgc caggcggcag gtttgctact gtcgatactc aactgcacca
    42481 tcgggatatc ggcgtcagga tacatcttaa tcagcacgcc ccaggaaccg tggtcaaagc
    42541 cccaggcttc tttatccagc gtcaccggga ccgacgctaa cagctcaacc agatgctgtg
    42601 ccagcacagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac
    42661 caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc
    42721 agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc
    42781 agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca
    42841 atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc attttgatat
    42901 cctcacaata cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag
    42961 ttattttgac gatctgcctg aaggtgaaga tttataagga gttgtcgatg tcagtacctc
    43021 tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg
    43081 ggcaaaaacc ctcgaaccgc ttactggcgt tttcgctagg atttgcggcg gggatcatgt
    43141 tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga atgtcgcctg
    43201 tgttgggtta tggaatgttt atcttcggtc tgcttggcta ttttggcctg gaccgcatgt
    43261 tgccacatgc gcatccgcag gatttaatgc aaaaatcggt gcagccgttg ccaaaatcga
    43321 tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga
    43381 ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg
    43441 tcgcgttgca caatatccct gaaggtctgg cagtggcagg cccggtttat gcggcaacgg
    43501 ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg actagcagaa attcttggtg
    43561 gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcggcaatca
    43621 tggcggcggt tgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca
    43681 aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg
    43741 gattcagttt agtgctgcta caaacggcgg gaattggtta aaaacaacat cagaagttgt
    43801 ccagcacatg gacaacttct gccagcttca cgaaagcctt attctacctt ttcggctgtc
    43861 tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg
    43921 cgaaacgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac cagagcaatc
    43981 aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa caatactaaa
    44041 catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt cgcataataa
    44101 cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt
    44161 ctcatagaaa gagaagcgga attaacatct tgcatagcag ggcaaaataa aaaccgaagc
    44221 cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt cagctggctt
    44281 tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc gccatattgt
    44341 gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca
    44401 gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca atagtcgctt
    44461 cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc
    44521 ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa
    44581 tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa
    44641 aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca agttgtttac
    44701 ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca
    44761 tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt tcacggtctt
    44821 catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac
    44881 gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact
    44941 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    45001 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    45061 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    45121 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    45181 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    45241 cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa actacaatga
    45301 ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa
    45361 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga
    45421 cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga
    45481 ctcgtgagaa actggcgcgg cttgaacaaa gaatgagcga actggaaaat cgcagtactg
    45541 agattaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca tttgtcttta
    45601 atgcactgta aattatgaat taatcttcta attta
//
LOCUS       DAZOZD010000006        43713 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOZD010000006 DAZOZD010000000
VERSION     DAZOZD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.006"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHUPB_000254"
     CDS             complement(<1..559)
                     /locus_tag="ACHUPB_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0757179.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUPB_000255"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUPB_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0757180.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUPB_000256"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUPB_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0757181.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUPB_000257"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUPB_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0757182.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHUPB_000258"
     CDS             complement(4001..6322)
                     /locus_tag="ACHUPB_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0757183.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUPB_000259"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUPB_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0757184.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUPB_000260"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUPB_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0757185.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUPB_000261"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUPB_000261"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0757186.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUPB_000262"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUPB_000262"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0757187.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHUPB_000263"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHUPB_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUPB_000264"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUPB_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0757188.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUPB_000265"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUPB_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0757189.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUPB_000266"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUPB_000266"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0757190.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUPB_000267"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUPB_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0757191.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUPB_000268"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUPB_000268"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0757192.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUPB_000269"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUPB_000269"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0757193.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUPB_000270"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUPB_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0757194.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUPB_000271"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUPB_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUPB_000272"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUPB_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0757195.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUPB_000273"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUPB_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR0757196.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUPB_000274"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUPB_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0757197.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUPB_000275"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUPB_000275"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0757198.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUPB_000276"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUPB_000276"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0757199.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHUPB_000277"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHUPB_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0757200.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHUPB_000278"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHUPB_000278"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0757201.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUPB_000279"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUPB_000279"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0757202.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHUPB_000280"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHUPB_000280"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0757203.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUPB_000281"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUPB_000281"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0757204.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUPB_000282"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUPB_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0757205.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHUPB_000283"
     CDS             32202..32564
                     /locus_tag="ACHUPB_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0757206.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHUPB_000284"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHUPB_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHUPB_000285"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHUPB_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHUPB_000286"
     CDS             35034..35912
                     /locus_tag="ACHUPB_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0757207.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHUPB_000287"
     CDS             35923..36276
                     /locus_tag="ACHUPB_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0757208.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHUPB_000288"
     CDS             36288..37592
                     /locus_tag="ACHUPB_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0757209.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHUPB_000289"
     CDS             37604..38689
                     /locus_tag="ACHUPB_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0757210.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUPB_000290"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUPB_000290"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0757211.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUPB_000291"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUPB_000291"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0757212.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUPB_000292"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUPB_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUPB_000293"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUPB_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0757213.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="ACHUPB_000294"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="ACHUPB_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR0757214.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11126 c        11988 g        10442 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cattcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaactcg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DAZOZD010000007        43203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOZD010000007 DAZOZD010000000
VERSION     DAZOZD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.007"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..505
                     /locus_tag="ACHUPB_000295"
     CDS             <1..505
                     /locus_tag="ACHUPB_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0757215.1"
                     /translation="KCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETKTVN
                     IISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVG
                     AAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPN
                     TKCPEKS"
     gene            521..1060
                     /locus_tag="ACHUPB_000296"
     CDS             521..1060
                     /locus_tag="ACHUPB_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0757216.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1155..2732)
                     /gene="guaA"
                     /locus_tag="ACHUPB_000297"
     CDS             complement(1155..2732)
                     /gene="guaA"
                     /locus_tag="ACHUPB_000297"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0757217.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2801..4267)
                     /gene="guaB"
                     /locus_tag="ACHUPB_000298"
     CDS             complement(2801..4267)
                     /gene="guaB"
                     /locus_tag="ACHUPB_000298"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0757218.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4429..5799
                     /gene="xseA"
                     /locus_tag="ACHUPB_000299"
     CDS             4429..5799
                     /gene="xseA"
                     /locus_tag="ACHUPB_000299"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0757219.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5796..6011)
                     /locus_tag="ACHUPB_000300"
     CDS             complement(5796..6011)
                     /locus_tag="ACHUPB_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0757220.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6080..7552)
                     /gene="der"
                     /locus_tag="ACHUPB_000301"
     CDS             complement(6080..7552)
                     /gene="der"
                     /locus_tag="ACHUPB_000301"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0757221.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7670..8848)
                     /gene="bamB"
                     /locus_tag="ACHUPB_000302"
     CDS             complement(7670..8848)
                     /gene="bamB"
                     /locus_tag="ACHUPB_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0757222.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8859..9479)
                     /gene="yfgM"
                     /locus_tag="ACHUPB_000303"
     CDS             complement(8859..9479)
                     /gene="yfgM"
                     /locus_tag="ACHUPB_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0757223.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9497..10771)
                     /gene="hisS"
                     /locus_tag="ACHUPB_000304"
     CDS             complement(9497..10771)
                     /gene="hisS"
                     /locus_tag="ACHUPB_000304"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0757224.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10882..12000)
                     /gene="ispG"
                     /locus_tag="ACHUPB_000305"
                     /gene_synonym="gcpE"
     CDS             complement(10882..12000)
                     /gene="ispG"
                     /locus_tag="ACHUPB_000305"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0757225.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12027..13040)
                     /gene="rodZ"
                     /locus_tag="ACHUPB_000306"
     CDS             complement(12027..13040)
                     /gene="rodZ"
                     /locus_tag="ACHUPB_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0757226.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13325..14479)
                     /locus_tag="ACHUPB_000307"
     CDS             complement(13325..14479)
                     /locus_tag="ACHUPB_000307"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0757227.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14629..15060)
                     /gene="ndk"
                     /locus_tag="ACHUPB_000308"
     CDS             complement(14629..15060)
                     /gene="ndk"
                     /locus_tag="ACHUPB_000308"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0757228.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15209..17521)
                     /gene="pbpC"
                     /locus_tag="ACHUPB_000309"
     CDS             complement(15209..17521)
                     /gene="pbpC"
                     /locus_tag="ACHUPB_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0757229.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17522..22483)
                     /gene="yfhM"
                     /locus_tag="ACHUPB_000310"
     CDS             complement(17522..22483)
                     /gene="yfhM"
                     /locus_tag="ACHUPB_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR0757230.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22690..23535
                     /gene="sseA"
                     /locus_tag="ACHUPB_000311"
     CDS             22690..23535
                     /gene="sseA"
                     /locus_tag="ACHUPB_000311"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0757231.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23871..23996
                     /gene="timP"
                     /locus_tag="ACHUPB_000312"
     CDS             23871..23996
                     /gene="timP"
                     /locus_tag="ACHUPB_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0757232.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24028..24804)
                     /gene="sseB"
                     /locus_tag="ACHUPB_000313"
     CDS             complement(24028..24804)
                     /gene="sseB"
                     /locus_tag="ACHUPB_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0757233.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24946..26229)
                     /gene="pepB"
                     /locus_tag="ACHUPB_000314"
     CDS             complement(24946..26229)
                     /gene="pepB"
                     /locus_tag="ACHUPB_000314"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0757234.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26407..26607)
                     /gene="iscX"
                     /locus_tag="ACHUPB_000315"
     CDS             complement(26407..26607)
                     /gene="iscX"
                     /locus_tag="ACHUPB_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0757235.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26619..26954)
                     /gene="fdx"
                     /locus_tag="ACHUPB_000316"
     CDS             complement(26619..26954)
                     /gene="fdx"
                     /locus_tag="ACHUPB_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0757236.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26956..28806)
                     /gene="hscA"
                     /locus_tag="ACHUPB_000317"
     CDS             complement(26956..28806)
                     /gene="hscA"
                     /locus_tag="ACHUPB_000317"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0757237.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28823..29338)
                     /gene="hscB"
                     /locus_tag="ACHUPB_000318"
     CDS             complement(28823..29338)
                     /gene="hscB"
                     /locus_tag="ACHUPB_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0757238.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29434..29757)
                     /gene="iscA"
                     /locus_tag="ACHUPB_000319"
     CDS             complement(29434..29757)
                     /gene="iscA"
                     /locus_tag="ACHUPB_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0757239.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29774..30160)
                     /gene="iscU"
                     /locus_tag="ACHUPB_000320"
     CDS             complement(29774..30160)
                     /gene="iscU"
                     /locus_tag="ACHUPB_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0757240.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30188..31402)
                     /gene="iscS"
                     /locus_tag="ACHUPB_000321"
     CDS             complement(30188..31402)
                     /gene="iscS"
                     /locus_tag="ACHUPB_000321"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0757241.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31643..32131)
                     /gene="iscR"
                     /locus_tag="ACHUPB_000322"
     CDS             complement(31643..32131)
                     /gene="iscR"
                     /locus_tag="ACHUPB_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0757242.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32432..33169)
                     /gene="trmJ"
                     /locus_tag="ACHUPB_000323"
     CDS             complement(32432..33169)
                     /gene="trmJ"
                     /locus_tag="ACHUPB_000323"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0757243.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33288..34091
                     /gene="suhB"
                     /locus_tag="ACHUPB_000324"
     CDS             33288..34091
                     /gene="suhB"
                     /locus_tag="ACHUPB_000324"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0757244.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34236..35090
                     /locus_tag="ACHUPB_000325"
     CDS             34236..35090
                     /locus_tag="ACHUPB_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0757245.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35281..36561
                     /gene="csiE"
                     /locus_tag="ACHUPB_000326"
     CDS             35281..36561
                     /gene="csiE"
                     /locus_tag="ACHUPB_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0757246.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36553..37692)
                     /locus_tag="ACHUPB_000327"
     CDS             complement(36553..37692)
                     /locus_tag="ACHUPB_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0757247.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37852..38742)
                     /gene="hcaR"
                     /locus_tag="ACHUPB_000328"
     CDS             complement(37852..38742)
                     /gene="hcaR"
                     /locus_tag="ACHUPB_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0757248.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38878..40239
                     /gene="hcaE"
                     /locus_tag="ACHUPB_000329"
     CDS             38878..40239
                     /gene="hcaE"
                     /locus_tag="ACHUPB_000329"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0757249.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40236..40754
                     /gene="hcaF"
                     /locus_tag="ACHUPB_000330"
     CDS             40236..40754
                     /gene="hcaF"
                     /locus_tag="ACHUPB_000330"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0757250.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40754..41074
                     /locus_tag="ACHUPB_000331"
     CDS             40754..41074
                     /locus_tag="ACHUPB_000331"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0757251.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41071..41883
                     /gene="hcaB"
                     /locus_tag="ACHUPB_000332"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41071..41883
                     /gene="hcaB"
                     /locus_tag="ACHUPB_000332"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0757252.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41893..43095
                     /gene="hcaD"
                     /locus_tag="ACHUPB_000333"
     CDS             41893..43095
                     /gene="hcaD"
                     /locus_tag="ACHUPB_000333"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0757253.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10244 a        11942 c        10848 g        10169 t
ORIGIN      
        1 aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat gccagtcaaa
       61 tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca aacaagaaac
      121 taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa
      181 taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg
      241 ccacaacgtc ggttctggca gcaattccgg aacgacggca ggggcagttg gcggcggagc
      301 tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt
      361 aacatataag gaaggcacca aagtgtatac ctccacccag gtgggtaaag agtgccagtt
      421 tacgacaggt ttagccgttg ttattaccac aacgtataac gaaacgcgta ttcagccaaa
      481 tactaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag tttttctttg
      541 cgccatctta gcctccttaa gctatccggc tatcgcctca tcattgcagg atcaactctc
      601 tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc atgacgaatg
      661 ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa atgcccaggc
      721 cgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc aggataaact
      781 ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg agctacgcag
      841 cttagagatt cagaaacaaa aactgacgct ggcgaaagaa gaagcccgcg ttaagcgaga
      901 aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg tggtgcaatc
      961 tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga aaagcgtggg
     1021 caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttag taacttcaag
     1081 cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt tcattcatag
     1141 tgcagggatt aaaatcattc ccactcaatg gtagctggcg gcttgccgct gatgtcatac
     1201 accacgcggg aaataccgtt cacttcattg ataatgcggt tggaaacgcg accgaggaaa
     1261 tcgtacggca gacgcgccca gtgtgcggtc ataaagtcga tggtttcgac agcacgcaga
     1321 gagacaaccc agtcatactt acgaccatcg cccattacgc caacggaacg taccggcagg
     1381 aacacagtga acgcctggct gactttgtcg tacaggtccg ctttacgcag ttcttcaatg
     1441 aagatggcgt cagcacggcg cagcaggtca cagtactctt tcttcacttc acccagcaca
     1501 cgaacgccaa ggcctggtcc cgggaatggg tgacggtaca acatgtcgta cggcaggccc
     1561 agctccagac caatcttacg cacttcgtct ttgaacagct ctttcagcgg ttcaaccagg
     1621 cccatcttca tctctttcgg caggccgccc acgttgtggt gagatttgat gacgtgtgct
     1681 ttaccggttg cagacgccgc agattcgata acgtcagggt agatggtgcc ctgcgccagc
     1741 cacttcacgt cttccagttt cagcgcttct tcatcgaata cttcaacgaa aacgcgaccg
     1801 atgattttac gttttgcttc cggatcgttt tcgccagcca gcgctgacag gaagcgatct
     1861 tctgccggaa cgtgaacaat gttcagacca aagtgatcgc caaacatatc cagaacctgc
     1921 tctgcttcgt tgagacgcag caggccgttg tcgacgaata cgcaagtcag gtttttaccg
     1981 atagcgcggt gcagtagcat tgcggttacg gaggaatcca cgccaccaga gaggccgagg
     2041 atgactttat cgtcgcctac ctgctcacgg atgcgggcta cagcatcgtc gataattttc
     2101 gctggcgtcc acagggcttc acactggcag atatcacgca caaaacgctc cagcatgcgc
     2161 ataccctggc gggtgtgagt cacttccggg tggaactgta cgccatagaa gcgtttttct
     2221 tcgttagcca taatggcaaa cgggcagctt tcggtgctgg ctacggtgac gaagtcggac
     2281 gggatagcgg taactttatc gccgtggctc atccagacat cgagcagcgg tttaccgtct
     2341 gcggtcagcg catcttcgat accgcgaacc agtgcactgt cgtttacgac ttcaacctgc
     2401 gcgtagccaa attcacgttc gttagaggct tcaacgtgac cgcccaactg cattgccatg
     2461 gtctgcatgc catagcaaac gccgaatacc ggtacgcctg cttcaaagac atactgcggc
     2521 gcacgcgggc tgttttcttc agtagtactt tccgggccgc cggaaagaat aatgccgctt
     2581 ggattgaagt cacgaatttg tgcttctgtc acatcccacg cccacagttc gcagtaaaca
     2641 cccagctcac gcacgcggcg cgcaaccagt tgagtgtact gagaaccgaa gtctagaatg
     2701 aggatgcgat gcttatgaat gttttccgtc attgacgctt attccgaggc aagtgaaaca
     2761 gataatataa atcgcccgac atgaagtcgg gcgaagagaa tcaggagccc agacggtagt
     2821 tcggggactc tttagtaatg gtcacgtcgt gaacgtggct ttcctgaatg cccgcaccgc
     2881 tgatacgtac aaactccgct ttagtacgca gttcgtcgat agtaccacag ccggtcagac
     2941 ccatacagga gcgcaggccg cccatctgct ggtgaatgat ctctttcagg cgacctttat
     3001 aagctacgcg accttcgata ccttccggca ccagtttgtc ggcagcgtta tcgctctgga
     3061 aataacggtc agaggaacct ttggacatcg cgcccaggga acccatacca cggtaagatt
     3121 tgtaagaacg gccctggtag agttcgattt cacccggaga ttcttcagta cccgccagca
     3181 tggaacctac catcaccgcg cttgcgccag cggcgatagc tttggcgatg tcgccggaga
     3241 agcgaatacc accatctgcg ataaccggaa taccggtgcc ttccagggct tcaactgcgt
     3301 cagcaacggc agtgatctgc ggaacaccta cgccagtaac gatacgagtc gtacagatag
     3361 aaccagggcc gataccgact ttaaccgcgc tgcaaccagc ttctgccaaa gcgcgtgcac
     3421 ctgcagctgt tgccacgttg ccgccgatga tttgcagatc cggatattta gcacgggttt
     3481 cacggatacg ctgcaaaacg ccttctgagt gaccgtggga ggagtcgatc agcagaacgt
     3541 caacgccagc ggcaaccagc gcgtcaacac gctcttcgtt acccgcacct gcgccaactg
     3601 cagcaccaac acgcagacgg ccttgctcgt ctttacaggc gttcggttta cgttccgctt
     3661 tctggaagtc tttcacggtg atcatgccga tcaggtggaa ttcgtcatca accaccagcg
     3721 ctttttcaac gcgtttttcg tgcatttttg ccagcaccac ttcacgggct tcaccttcac
     3781 gcacggtgac cagacgctct ttcggcgtca tgtaaacgct aaccggctgg ttcaggtcgg
     3841 taacaaaacg cacgtcacga ccggtgataa tacccaccag ttcgttttct tcggtaacga
     3901 ccggatagcc cgcaaaaccg ttacgctcgg tcagttcttt cacttcgcgc agcgtcgtgg
     3961 ttggcagaac agtctgcgga tcagtcacca caccagattc gtgttttttc acacggcgaa
     4021 cttcttctgc ctggcgttca atggacatgt ttttgtggat aaagccgata ccgccttcct
     4081 gagccagagc aatagccagg cgcgcttccg ttacggtatc cattgctgcg gaaagcatag
     4141 ggatattcag acgaatagtt ttcgtcagct gggtgctgag gtcagcagta ttcggcagaa
     4201 cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt cagagcttct ttagcgatac
     4261 gtagcatggg caatatctcg accagagtgg ttaataaata ttgccgcggc attatacaga
     4321 gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct tgctatcccc gaagggcggg
     4381 ttactatcga ctgaataacc tgctgattta gaatttgatc tcgctcacat gttaccttct
     4441 caatcccctg caatttttac cgttagtcgc ctgaatcaaa cggttcgtct gctgcttgag
     4501 catgagatgg gacaggtttg gatcagcggc gaaatctcta atttcacaca accggcttcc
     4561 ggtcactggt actttacact caaagacgac accgcccagg tacgctgcgc gatgttccgc
     4621 aacagcaacc gccgggtgac cttccgtcca cagcatggac aacaagtttt agttcgcgcc
     4681 aatattacgc tctacgagcc gcgcggtgac taccagataa tcgttgagag tatgcagccg
     4741 gccggtgaag ggctgctgca acagaagtac gaacagctca aagcgaagtt gcaggctgaa
     4801 ggtttgttcg atcagcaata caaaaaacca cttccctccc ctgcgcattg cgttggtgtg
     4861 atcacctcaa aaaccggtgc tgcgctacat gatattttgc atgtgttaaa acgtcgcgat
     4921 ccttctctac cggtgatcat ctaccccacc gccgttcagg gcgatgacgc accggggcaa
     4981 attgttcgcg ccattgaact ggcgaatcag cgcaatgagt gcgacgtgtt gatcgttggg
     5041 cgcggcggcg gttcgctgga agatttatgg agttttaacg acgaacgcgt agcgcgggcg
     5101 atttttgcca gccgcattcc ggtcgtcagc gccgtcgggc atgagacgga tgtgaccatt
     5161 gccgattttg ttgccgatct gcgtgcgcca acgccgtctg ccgccgctga agtagtgagc
     5221 cgtaatcagc aagagttact gcgccaggtg caatcgaccc gtcaacggct ggagatggcg
     5281 atggattatt atctcgccaa ccgcacgcgt cgctttacgc agatccatca ccgattacag
     5341 caacagcatc cgcagctccg gctggcacgc cagcaaacca tgcttgagcg cctgcaaaag
     5401 cggatgagct ttgcgctgga aaatcaactt aagcgtaccg ggcaacagca gcagcggtta
     5461 acacagcggc tgaatcagca aaatccacag ccgaagattc atcgcgcgca aacgcgcatt
     5521 cagcaactgg aatatcgttt agcagaaacc ctgcgcgtac agcttagcgc cacgcgtgaa
     5581 cgtttcggta atgcagtaac gcacctcgaa gccgtaagcc cactgtcaac gctggcgcgt
     5641 ggatacagcg ttactactgc tactgacggc aatgtactga aaaaagtgaa gcaagttaaa
     5701 gcgggtgaaa tgctaaccac acgtctggaa gacggctgga tagaaagtga agttaaaaac
     5761 atccagccag taaaaaaatc gcgtaaaaag gtgcattaag ccagcacgaa ttcaacgcgt
     5821 tttttcgata tcaagccatg cccgtgctga cagaagtaat ctactgcacc gcaggctttt
     5881 aacacctgca acggttgatg gcagtctgga caaagagctt tcatttcaat aatttccccg
     5941 caggatgggc aacgcgcatg gccgttatcc tgatcaagca catgctgaca ttgtggacaa
     6001 tgcagttcca tatgaattcc tccggtagcc atccctctac attcatagag ggaatggcag
     6061 ataaaatact tacggataat tatttatttt tcttgatgtg cttcatcaga cgcttacgtt
     6121 tacgcatctg ggttggcgtc agggtgttac gcttgttcgc atacgggttt tccccttctt
     6181 tgaactgaat acgaatcggc gatcccatta cgtccagcga tttgcggaag tagttcatca
     6241 agtagcgctt gtaggaatca ggcaggtctt tcacctgatt accgtgaatc accacaatcg
     6301 gcgggttata accaccagcg tgggcatatt tcagcttcac acgacgaccg cgtaccagcg
     6361 gcggttggtg atcttcaaca gccatcgtca tgatgcgcgt cagcatagag gtccccacac
     6421 gacgggtgga gctgtcatac gcttcacgta ctgattcaaa caagttacca acaccactgc
     6481 cgtgcaaggc agagataaag tgcacacgag caaaatcgat aaagcccaga cggaagtcca
     6541 gcgtttcttt cacctgctct ttcacttcct ggctcaggcc atcccactta ttcaccacaa
     6601 tgacaagtga gcgcccacta ttgagaataa agcccagcag cgagagatcc tgatcggaaa
     6661 taccttcgcg cgcatcaatc actaacatca ccacgttggt atcttcaatt gcctgcaacg
     6721 ttttgattac ggagaatttc tctacagcat cggtgatttt gccgcgttta cgtacgccag
     6781 cggtgtcaat gagcacatac tcacgtccat cgcgttccat tggaatgtag atactgtcac
     6841 gcgtcgtgcc aggcatgtca taaacaacaa cgcgctcttc accaagaata cggttagtga
     6901 gtgtagactt acctacgttc ggacgaccca caatcgccag tttgatcggc agactttgcg
     6961 ggtcgaagtc gtcttcctct tcttcttcgc cgttctcttc cgcttcaaat tgcgcccagt
     7021 attcagcgtc ttcgtcgact tcctcttgcg gtgcgagatc ttccatccac ggcagcagca
     7081 cacgctccag cagacttaat acgccacgac cgtgagatgc ggcgatcggg tagatttcac
     7141 ctaaaccaag cgagtagaaa tcaaccactg cctgatcggg atccagaccg tcagttttgt
     7201 ttgccaccag gaaggtcggt ttttcacgag agcgcagatg tttggcaatc gcttcatctg
     7261 ccggcatcaa gcccgcgcgc gcatccacca taaacagtac gacgtccgct tcttcaatcg
     7321 ccagcagcga ctgttccgcc atgcgggttt ctacaccgtc ttctgtgcca tcaatcccgc
     7381 cggtatcaat acagataaac tcacggcctt caatttccgc acgaccgtac ttacggtcac
     7441 gagtcagacc cgggaaatcc gcaaccagcg catctcgggt gcgagttaga cggttaaata
     7501 acgtggattt tcctacgtta gggcgcccga caagcgcgac cacaggtacc atgtttaaag
     7561 cctcattttt ataaatcatc agacaacgca cgctatattc gcgtcgttgt taaaaacagg
     7621 aaaacggccc ctgtccagga gccgttttca aagtgaacga cagagacgat taacgtgtaa
     7681 tagagtacac ggttccgtct tttgcctgga tcagcagttt gccgtcagcg gcaaccggtt
     7741 cagtctggaa accggaacta tcaacttttt gctgggcaac gaaacgacca tcttcgacgt
     7801 taatccagtg cagataacct tcactgtcac ctaccaccag gttgccatta tacagcaccg
     7861 gcgaagtcag caggcgatgc agcagatcgc tttgtgtcca cagcgtaacg ccgccatcaa
     7921 tggtcaacgc catcacccgg tcattttgat cgaccagata gatgcgattg ccgtcgacga
     7981 tgaaatcatt caccgaaccc agttcgcgtt tccacataat ctgaccactg cgcagatcaa
     8041 gcgccgtcag gttaccatta taggccagag cgaaaacaac gccgttaaca acgacgggag
     8101 tcgtgtcaac atcgctcaga cggtcaattt cggtagaacc ggtcgcctgg gaaatacgct
     8161 gctgccaaat catctggccc tgttccatca gcactgcgct gacgcgacca ttatcgcccc
     8221 ccacgacggc cgcaccaaaa gccgttgccg gcgcagactc gccacgcaaa gagagcgaag
     8281 gcatatcgag gttaactgtc catttgacag cgccgtcagc ttcgttcagc gcttgtaact
     8341 gaccgttact agtgtggatt aacaccagac cgtcgctgac caccgggcga gaaagtgctt
     8401 cacccgcgac tttagtttgc catgccacag taccatcgct ggtattcagc gcgtaaacct
     8461 gcgccttttc gctgccaatg tagacatgcc caccagacac ggtcacaccg ccagaaagta
     8521 atgcaggctc tttagagaac cagccatctt tctcggccag gctgacagac cagatttctt
     8581 tgccatcatc cgcattcagc gcttttacca aaccagcgcg gtccgctgca tagacaacgt
     8641 tgtccgccag tgccggatga agattggaat agaagttgcc aatgccgcta ccaacggaag
     8701 tgctccacgc cgtggtcggc gtaaactggt tttcaacggt tggcaatggg gacatcttta
     8761 ccacatcttc ttcgctgtta aacagcgaac agccgcttaa aagggtaacg gaaagcagtc
     8821 ctggcagcag taatttacgc aattgcatcg ggtccctctc agatggacaa attattaatt
     8881 ttcatctgca tcatttcgct cagtgccgga gtaacatcgc ttttcacgcc tgcttcccat
     8941 gcactacgcg caccttgctt atcacctttg ctcaacaatg ctttaccacg caggtcggca
     9001 acaatggcag cccacccttc acctttgatg gtatcaaggg ttttcagcgc ggcatcagcc
     9061 tgcttgagct gtacctgaac gcgagcaaga cgcagattta tcacggcttt gagattttca
     9121 tcactcgtgt ctgccagccc ctgttgtaac tgggcggcag ctttctctag ttcatttttg
     9181 tcaacaaatt gctgcgcaag ttccaaagaa gccagcgcac cataagtatt tttattttca
     9241 gcagcaaatt tttccgccgc cgggatgcta tccggtttgc cttcgctcac tgcggtaacc
     9301 gcattttgat aggcaagaga agcggagcgt gcagaatcaa cctgatggct gttccagtag
     9361 cgccagccaa tcagtgcgcc aacgcccaaa atcaccccaa cagccagtgc tttgccattt
     9421 tcagcaaaaa agcgtttaac cgcttctacc tggtcgtttt cgttctcgta aatttccacg
     9481 ctgtccttct ccttccttaa cccagtaacg tgcgcaaatg cgcggctacg ctatcctgcg
     9541 caactgccgt ttgctcacca gagcgcaaat ccttcactac tgctgtgccg ttagccactt
     9601 cagactcacc cagcaccaca gcaacgcggg caccccattt atcagcacgg gcaaactgtt
     9661 tcttaaagtt gccgccgccg tggttggtca tcaatttcac gcccggtaat tcatcacgca
     9721 gacgctcagc taatgccata gccgcagatt gtgtatcagc acctgaagcc accaggtata
     9781 tatcgacaac aggatcggct ttaaattccg gattaacggc ctgtactaac aatacaagac
     9841 gttcgaggcc catcgcaaaa ccgactgccg gtgttgcacg accgcccagt tgttccacca
     9901 gaccgtcata acgaccgcct gcacacacgg tgccctggga gccgaggctg ttagtcaccc
     9961 actcgaaaac ggtacggtta tagtaatcca gaccacgcac cagacgctgg tttacggtgt
    10021 aagcgatccc cgcgctctca agcagtttgc acagaccggc aaaatgctca cgagattcct
    10081 cgtccagata atcacctaat gccggagcgt cgttgagaag cgcctgcact tccggatttt
    10141 ttgaatccag cacgcgcagc gggttagtgt acatgcggcg tttgcagtct tcgtccagct
    10201 tttctttatg ctgctcaagg aatgccacca gcgcatcgcg gtaattggcg cgtgcttcca
    10261 gcgaaccaat agagttcagc tcaagagtta cgtgttcgaa gatacccagc gcacgccacc
    10321 agcgggcagt gagcataatc agttcagcgt cgatatccgg accttgcaga ccgaaaactt
    10381 cgcagcccaa ctgatggaac tgacgataac gccctttctg cggacgctcg tgacggaaca
    10441 tcggcccgat ataccacaga cgctgttcct gattgtacag aagaccatgc tcgatgccgg
    10501 cgcgtacaca gcccgccgtc ccttcagggc gcagagtcag gctgtcgcca ttgcgatcct
    10561 caaaggtgta catctctttt tcaaccacgt cggtgacttc accaatcgca cgtttgaata
    10621 gcggggtctg ctctacaatc ggcaagcgga tttcactgta accgtagctg ccgagcacgt
    10681 ttttcagtgt gccttcaatg cgctgccaga tggccgtttc gccaggcagg taatcgttca
    10741 tgccgcgaat ggcttgaatg ttttttgcca cgtttattct ctttctgaat ataaaaatga
    10801 accctcaacg cttccctcaa tgtttcggga gccatgcggg ttcaatcata cacgggaagc
    10861 gaggcgcttc ccatcacgtt attatttttc aacctgctga acgtcaattc gacgcgcttc
    10921 gtccagctga ctggctttcg cacgaatgcg tgcttccagc tggtcgatca tatcgttgtt
    10981 gtccagacgg tctttgcgca cgccatcttc atagaggccg cttttcttgt tgccgccggt
    11041 gacgccgagt gtagaaacca gcgcctcacc tgggccattc accacgcagc cgataatcga
    11101 aacgtccatc ggagtgatga tatcttccag gcgttgctcc agcgcgttaa ccgtaccgat
    11161 aacatcaaat tcctgacgcg aacaggtcgg gcaggcgatg aagttaatcc ctcgcgaacg
    11221 gatacgcagc gatttcaaaa tatcgaaacc gactttgatc tcttcgaccg gatcggccgc
    11281 cagcgatacg cgcagcgtgt cgccgatgcc ttccgacagc agcagaccta aaccaatggc
    11341 ggattttact gccccgctgc gtgcaccacc ggcttcggtg atccccagat gcagcggctg
    11401 atcgatctgt tttgccagca aacgataaga ctcaacagcg aggaagacgt cagacgcttt
    11461 cacgctgact ttgaactgat cgaagttcag gcgatcgaga tgatcaacat gacgcatggc
    11521 ggattccagc aacgcctgcg gcgtcggttc gccatacttt tcttgcagat ctttttccag
    11581 cgatccggcg ttaacgccaa tacggatcgg aatgttttta tcgcgcgcac agtcaaccac
    11641 catgcgaata cgctcttcat taccgatatt gccagggtta atacgcagac aatcgacgcc
    11701 gtattccgct actttcagcg caatgcgata gtcgaagtgg atgtcagcca ccagcggcac
    11761 gttaacccgc tgtttgatga gtttgaacgc ttctgccgcg tccatcgtcg gtacggagac
    11821 acggacgata tcagcgccaa cgcgttccag cgccttgatt tgattgaccg ttgcttcgac
    11881 atctgtcgta cgcgtattgg tcatggactg tacggcgatg ggagcaccat cgccaatcgg
    11941 cacattccca acgtaaatac gtgttgattt tctacgttga attggagcct ggttatgcat
    12001 gaaaaatctc ccgcgttacc cgtctgttac tgcgccggtg attgttcggc attgagggtc
    12061 agacgcgcaa cctggttagt tctgataaaa cgactcagat cgacaggttt cccttgatac
    12121 tggatctgta ctgcggctgg cgcaccaatt ttcagtttat acggtgcctg gccggttaaa
    12181 ttcaaattac cgtctttacg ctgcatcccg ctaaacaatt ttttaccggt agcatcagtg
    12241 acctccagcc agcaatcggc agtaaagttc atcaccagcg cattcggatc agccgccggc
    12301 gtggtcacgc cagcctgatc ggttggcaac ggcgcagcac catctggcgt tgttgctgcc
    12361 gttggtaccg gggtcgcggc ggtatcaaca tttgcctgcg aaggcgaaac aaccgcattc
    12421 tgttgcggat caacagctgg tgctggcgca gttacggcag gtgtttgcgt gttggttgcg
    12481 gtagtatcca cagacgctgg cggcgtggac gttgtagccg ggtctgtagt tgtcgacgta
    12541 tttaacggga cactctgccc ctgctcgcta ttactgctca gttctgccga agattgatcg
    12601 gccatagtgg tgatctcttc ctgctgagct ttgtggtctt gccaccacca ggcaccgctc
    12661 aggccgataa ccacaaacaa caccagccag gtgaaggtca tcagccagcc atcacgtttt
    12721 ttgcggcgtt taccgaggga aaaactctgc attggcgcaa cttttgcagc ccgtagtgga
    12781 gcctgctttt ccagccctgg cagcagttct tcttctggaa tatgtaccag acgcgcataa
    12841 gagcggatat atccgcgcag gaatgttgaa gcaagatcgg cgggtgcctt atcttcttca
    12901 atgtcgcgta ccgtggaaac cttcaggcaa agtcgctcgg caacggcctg ctgactaagt
    12961 cctagttgtt cgcgagcatt acgcaggcga gcgccggtag taagtgcttc attttggtcg
    13021 tgcgtggctt cagtattcat tcgctgcagg tacgtttaaa tgagaattga gatgccggtg
    13081 aaccatcatg ctcacccaca ccgcgaaaca tccgttaagt taaccgttat catacagtat
    13141 aagaccgtct gtgtgctcgt gacaaagccc acattcacta aggctaactt actgttgcat
    13201 cgttacatac tgccttaaag tcagcaaaaa cgcaccgtta atattgacca gacaattgca
    13261 acttaatact tcattcataa aacattacgc cacggtacat aaagtaaccg tggcgtaatg
    13321 gctatcagac cgctttaatg tcgatggctt caccctgcat ccgtttacgc agggtacgtt
    13381 tcgtacggtc gataacatcg cccgccaact gaccacaggc agcatcgata tcatcaccac
    13441 gagttttacg cacaatagtg gtgaaaccgt agctcatcag cacttttgag aaacggtcga
    13501 tacggctgtt cgagctgcgt ccatacggcg cacccgggaa cgggttccac gggatcaggt
    13561 tgatcttaca cggcgtatct ttcagcagtt ccgccagttg gtgcgcgtgt tcagtgccgt
    13621 cgttaacgtg gtcaagcatc acgtattcaa tagtgactcg gccctgattg gcgttggatt
    13681 tctccagata acggcgcacc gcagcaagga acgtttcgat attgtacttt ttgttgatcg
    13741 gcacaatttc gtcacgaatt tcgtcgttcg gcgcgtgcag ggaaattgcc agtgcaacgt
    13801 cgatcatatc gcccagttta tccagcgccg gaactacacc ggaagtagaa agcgtgacgc
    13861 gacgtttaga caggccaaaa ccgaaatcat caagcatgat ttccatcgcc ggaacgacgt
    13921 tgttcaggtt gagcagcggc tcgcccatgc ccatcatcac tacgttagtg attggccgct
    13981 gaccggtgac ttttgctgcg ccgacgattt tcgccgcccg ccacacctgg ccgataattt
    14041 ccgacacccg caggttgcgg ttaaagccct gctgggcggt ggaacagaat ttacactcca
    14101 gcgcacaccc cacctgcgaa gagacgcaga gcgtggcacg gtcgtcttcc gggatataca
    14161 ccgtttcgac gcgctgatcg ccaacggcga tcgcccattt aatggtgccg tcggatgaac
    14221 gctgttcttc aaccacttcc ggtgcgcgga tctccgccac ctctttcagt ttgccgcgca
    14281 acactttgtt gatgtcggtc atctcatcaa agttgtcgca gcaatagtga tacatccact
    14341 tcatcacctg atcggcgcgg aagggttttt cacctaaatc tttaaaaaac tcccgcatct
    14401 gctgacggtt gagatccagc aggttgattt ttccatcttt cgtggtgacg ttttcaggtg
    14461 tgactaattg ttcagacata tgctattccg gcctcgttat tacacgttat ggcccctgga
    14521 gggttgaaaa aagaaacgcc ccggtgagcg gctgctcgtc cgggggcgct gcattgtaca
    14581 aattctggcg cacggatgcc acgtttgcac gcggcattta cgaaattatt aacgggtgcg
    14641 cgggcacact tcgccttcgc cgaagaaata agcgatttcg cgagcggcag attcgacgga
    14701 atcagaaccg tgggtaccgt tttcggtcag gctgtcagcg taatcagcgc gcagagtacc
    14761 ggccagtgcg tttgccggat tggtcgcgcc cagcagatcg cggtgacgct gaacggcgtt
    14821 ttcaccttcc agcacggaaa ccacgatcgg gccagaggtc atgaattcaa ccagaccatc
    14881 aaagaacggt tttccatcgt gttcagcata aaagccacga gcctgttcaa cggtcaggtg
    14941 cagcattttg gtgccgacaa ttttgaaccc tgcagcttca aagcgcgcaa agatattacc
    15001 aatgacgttt tttgctaccg cgttcggttt gatgatggaa aaagtacgtt caatagccat
    15061 ttttacctct gtaaattgtt ctgttgttgt ctgtaccagc gtacgagatg gcgcggatta
    15121 taatgagcaa cagggccgtt gactattgat gaaggtaaca tttttttaaa ataaaatgag
    15181 ttttagcaac aatcagggac aaaacagact attgcatgac aaatttcact gtcgcgattt
    15241 gccccacgtc atccatcacc agcaattgat aatcaccttt atccgtcaaa tgcagggtca
    15301 cgttgcgccc gcgttcagtt aacggttcgc cattcagaaa ccaccagcgt tcacctgccc
    15361 ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatcg
    15421 cgccatcgcg aacgcctgtc agctgcagag ggagctgggc atcgtgaccg tatggcgggc
    15481 aaattgtcga ggccggtggt aagcgcacag cgcgacgttc tgatgcgggc agccagggtt
    15541 ccagcggtag cggccagaca tttaccattt cttgcttcgc ctgcgggcag tcagcggcga
    15601 cgcgctgccc tttgtcatcc agccagatgg ggaagcgaat gccattaatc ccttcctgct
    15661 ctgacagtaa cagcgttggc ggctggctgc catccagcag ccaggttgcc aggcggcggc
    15721 gacagttacc gtcaccttcc ggcaaagact gtccgcccgg ccagcagata acgccacgag
    15781 tgactgagtt cgggcgcggg tcttccggca gattcgcact gcgcgacagt aagatattat
    15841 tgacctgatt caacaatggt acggcactgg caaagccaaa ctgaccaaca acgggcgtgc
    15901 cgtcaggtct gccagtccag atcccaatga catagcgagc gttaacccca atcgcccagg
    15961 cgtcacgata gccatagctg gtgcccgttt tccatgccag tggggcgacg cgcggcaagg
    16021 cactatccgg caagggttgc gcttcatcag ccataatccg gcgaatgatc cacgccgccc
    16081 ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg
    16141 ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat atcttccagt tttgcaccag
    16201 cgccgccgag aatgagtgaa agattcggcg cagcgccgtt gggcaaatat aacggcaatc
    16261 caacattgcg taactttgcc gcaaaccgtt tcggtccata ggcttccagc acctgcacag
    16321 caggtaagtt cagcgagcgc accagcgcct cgctcatgct gatcgggcca tgaaaaccgc
    16381 tatcaaagtt acctggtcga taatcaccgg tgcgccgggg gacgtcttgc agcagtgatg
    16441 ccgggtggat caagccttca tccagcgcca gaccataaac aaacggtttg agcaccgatc
    16501 ctggcgatcg gatcgcattg accatatcaa catgaccaaa gcgtgaatca tcgttgagat
    16561 caaccgatcc cacccagccg cgaacacgca tatcggtatg atcaaccacg atcatcgcca
    16621 gtgagctgcg cggtggcaac cgccctttcc agttttgcgc cagttcttcc agacgtcgtt
    16681 gaagaccggc atccagcgta gtaacgattt tgtcgctttt gcttttaccg agcatcatgc
    16741 gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc cagccagatg ggttcttccc
    16801 ttgactcttt tacctgctca cgggaccaca caccttgcgc agccatccgt tcgagcactt
    16861 tattacgcgc ggcttcggca cgctccggcc aacgatccgg gcgaagacgg ctgggcgctt
    16921 gcggcaaaac cgccagcatt gccgcctcgg aatagcttaa attcgcaggc gactttccga
    16981 gataagccca acttgccgca ccgatcccct gcaacgtacc gccaaacgga gcgcggttaa
    17041 gatacaaggt cagaatttca cgcttagaca gatgccattc cagttgcaac gcgcgccaga
    17101 gctggcgaat tttgccgcca aatgttttgg ggtgaggatc aagcagacga gcaacctgca
    17161 tagtgagcgt gctgccaccg gaaataaccc gtcccgaagt gagatcttgc catgctgcgc
    17221 gcgccaccga gaatggattc acccccggat gcttccagaa ccagcgatct tcataattga
    17281 tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt taccggataa cgccagatgc
    17341 cgtcagcatc ggcgaagcgc cagagcggcg taccatcctg cgccacgacc actcgtgcgg
    17401 gattgacttc atgcagcggt agaggccaga gtttatctgc tccccacgct gcaagaaaaa
    17461 ggagaaatgg cgcagctgcc agcgttatcc agcagccgcg tttggttaac aagcgaggca
    17521 tttacggtcg gacaatcagc agatcttcag ccgcgccggt cgcccgccat tggggaacat
    17581 acattgattc caccatcggt tgcggtacct gatacgttcc cggcgtcacc gcccgcgcca
    17641 gatacaccag cgttaccggt tggtattcat caacggcaac cgccgccaca aagcgatcgt
    17701 cacggaactc aatgtgctta atactcgcct gctgcatctg gttcagtaag ttctgcactt
    17761 cgccaccact ttgctccagg ctggcgctac cgttcgccag attctggttt tccagttcca
    17821 gacccacagg cagcagatcc acgactaacg catccggcac gctgttactg gcttttacct
    17881 gcaaccacac cagcaccaga tcgccgctac gtaacgagtc cagcgatttg ctcttaccat
    17941 cagtaccaag aatatgacgc tcgatttgca gcacattgtt cgccggtaaa ggtgcggatt
    18001 gcggataacc gctggcatcc atacgcaacc ataacggctg atcgccactg ttgctcacct
    18061 gcaaggtgac aagttgatcg ctattcagat tgctgttttg cgctttctcg cctgtcagcg
    18121 gctcagctga gaaagaggtt tgcgcctgcc atttacccgg taaatcctga atcgtacggg
    18181 cagccaggaa caacgcgtta ctttcctgcg tcgatagcca gcgttcacca aacgcctgct
    18241 gcgaaagtgt gttcagcaaa gtgtactgct catcgggtag cagtttattt tcttccagca
    18301 aggagagcat taacgcgttg tcgcgcagtg gactaccgta atcacccagc catatccgct
    18361 catcactatt tctcggcgtt ttcagcgcca gcgcaatcgc ttcttcacca cgcgtcgcat
    18421 cacccatggt tttcagcgca atgccaagtt gcagcagcgg taaaccagaa gcggcatctg
    18481 cacgatgctc ccagatttca cgcagcgcac ccagtggtgc tttttgctga cgggccagca
    18541 ccagcgcagc gtaagactga acggcgaatt tactggcttt gagattatcc gcgtacggga
    18601 tcgacatcat gcccggatct tgtaaatagc gcagcagacg ctcattaccc cggttaatgg
    18661 cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac caggaaatcc atcacgtaag
    18721 ccgtcagcca gtactcttcg tcaccgtttt tatcccacag cgcaaagccg ccgttatcac
    18781 gttgcatttg cagcaaacgg gaaatgccga tatcgaccga tgcacggcgt ttctcatcac
    18841 tgtcgccttt gatgcccaac gcctgcagtt gggctgcgtt ggtataaagt gacgggaaca
    18901 ggccgctggc ggtttgctca agacagccgt acggatacgc ttttaactct ttgatataac
    18961 gtgcaatgtt cagcggtggt ttgccgctca acaacaattg cccttccagc gtaacaggcg
    19021 agaagttttg caatccatcc gccggaatcg cccatgtctc accaggctgt aacgccgtac
    19081 cgtaattaac cgtttgtgcc gggaacgccg gacggacgcc gattttccac tgcttatgct
    19141 gatcggcaac ggtttcaccc ggtaacgcta acccgctaat ggtggcctga atttctccat
    19201 cgccataacc cggcaatgct cgcaccggga taaacagcgt agtacgcaca cctggcgcta
    19261 attcaacggc tgcgggtgaa tcgctgacca gttcaagcaa accactggcg gtcagggcaa
    19321 cgttcagttt ttgcggttta tcggtaagat tagtgatatc cagcgtcaga cgcgaggtat
    19381 cgccactcgc cataaagcgc ggcatgttca gttcagcaat caccggtgcg gcaactatca
    19441 ctttgctttc gttgctaccg aaatcatctg ccgtccaggc ttgcgccatg acgcgcaatt
    19501 caccgttaaa atcgccaatc ggcagtgtaa ccgagccttc gccctgttcg ttgagcgtta
    19561 ccgacagcgc ctgctgcgcg acaatattga cgtgattgac cggcggttta ccaccacgtt
    19621 tcagctcatc accatcgcca ccgaaacgca gagctgccag acgcccctga ccttcaataa
    19681 cctgaccgta aatatcgtaa atgtctgcgc catagcgttt ctgaccaaag aacgcctgcc
    19741 acggatctgg cgtaacgtag tcagtaatat tcagcacacc actatcaacg gcagacacca
    19801 gcacattcac ctgtttaggc ttctcgccat ttttagtgct ggctttaatt ttcacggtta
    19861 atggttgatt gggacgcatt tttgctggtg tttccagcgc cagatcgagg cgacggtttt
    19921 catcaccaag cggcagatgc aacacaccaa ccgcgcgttt tggcgtcgcg gagcgagatt
    19981 tatcgccagg acgcaccacc agcgtactca aataaagatc atgacgattc caggttttat
    20041 cgaccggaat cgtcagatcc agcccttgag ccggaacatc aatctcttgc caccacagcg
    20101 gcccttcact ggactcgacc atcgcataac ctttacccgc cgttggcgcg gcaatatgca
    20161 acttaatggt atcgccgggg cgataactgg ctttatctag tttcagcgtg acacggtcgg
    20221 gtcgcactgc gccgctcccg tcgctgttgt cctgccagct atagccagcc caaaaacgca
    20281 cgctactgac cgcttcattc ggcgctttga cttccagacg ataagcgccc cactctaccg
    20341 ggaaactgac cttaccggtt tcatccgcct gaagatccag agtttgttca ttttcgatca
    20401 gatctttttg atcaaactgt gactgccagc cttcatcttc tgaccagttc cagtagtaat
    20461 cgcggcgttc gcgaatcagg cgcacctgca agcccgacac ggcttttttc acgccttgcg
    20521 catcgctata aacgatgtca aaagcggcgt tactgccttc atcaacaatg ggctgtttca
    20581 ccgtgctgtc agtgcgataa tcgtaaaccg atttcaaggc gaactgcgga cggagccccg
    20641 gcaatgcatc ggcaggccag atagcctgct cagcgcggcg cgtcaccggg cgaccgcccg
    20701 attccagcaa actgccctgg aaaataacct gcaatgggga atgcgtttcc ttccactggc
    20761 tttctgtaga aacttcgccg cgccctttat catccagcgt caactgaact tcatccagcg
    20821 tgcgggaaag gttttcggca gctatatcgc cgaattcaaa accaggtaag gctgacacag
    20881 cctcacgcag tgggcgcagg aaaagttgcc cttgcaaagt attaccatta gcaggcgcac
    20941 cgtacaggta gtatcccacc acggagaatt tcacttcatc attcggcgta agcggggttt
    21001 tctcaccggt cagattcagc gccatacgct ctggcataaa atcttcgacg tggaaatccc
    21061 acatccggta ctggttatcg cccgtgttgg cgcgaatatg ccacataccg gttgccgcat
    21121 tgctatcgag tggccaggta aagtggtaga ggccattctc cggctgacta acgacgttcc
    21181 tgagtacctg cccgtcaggt ttaatcacgt ctaacttgat gggttgatcg ggcaatgctt
    21241 taccgtctgc atcgcgcagt aaaccattga ggattaccgt ttcacctggg cgataaagat
    21301 cgcgtgggcc aaacatgaaa aactgtttgc tatagcctgg cgcgccagca atgttaaatt
    21361 ctgctaagtc cagcgccgga agttttaaat cgagtagcgt tgtctgaccg tctttacgtg
    21421 ccagcagtaa tgccgcattt ttatcatttt ccagctgcac atgcccctga gcgtcactgg
    21481 ttgcctgagt cagagtctgt cctttctcat ttaataaaga gacttcaatt ccttgctggg
    21541 ccgcgccgtt ttccagactt tgggtaaaga tatccagaca attgtgataa cggtgagctg
    21601 aaacgccgat atcacttaac gtaaacagcg tcgcgggatt actgtaatcg taacgtccag
    21661 cctgattcat cacagccaga tacacgcccg cctgctgaag cggtttgata tcgcccagcg
    21721 gcagcaataa tttttcacgg gtgttacgcg caggattgag atcaaaccgt ccggtgtaga
    21781 ccagatccgc catctgcagc agtttgtctg actgccagtt cgccagcgaa ttgcggtatt
    21841 cccattggct aatgaatgct ggcagagatt ctggcttaac gcggaagaag ttaacatcga
    21901 cattattaac gttgagcgcc attaccggca gcccttcaac gactttgcca ggcagcagcg
    21961 aaccacggct ggcaaaaccg acgctgggtt ggatgtcgcg ggtagttata gttttttcgt
    22021 aatctttact gaaggttgcg ttgttgagtg ctttgacctc cttgccaata gtaacgatca
    22081 aatcacgttt cggttcgagg tggcgtaaac gtagctcttt aagattatct gacagctccc
    22141 aggcaccatc cactttgccg ctttttttat cgacgacatg aataacgcgt gagaaatcct
    22201 gatccgggtc gagagggatg gagaacgtca gcaccagcgt ggctgcacca tcaagttgga
    22261 cttcagagag atccagcaat gtcagcacct taccggcact ctgttgggcc agtttttgtc
    22321 tctccggcac ggagagtttt gctgaactcg cgttttctga agaggccgct ttagtaactt
    22381 cagaaggcgc atcttttttc accgctgttg gcgcgttatc gttgttgtcg caccctgcca
    22441 gcgccagcat tagcatgcag gcggctacgc gtaacttttt catttttcat ccctgccaca
    22501 atggcccgtt agcaacgtcg aataattatt atgcgtgaga atttacgtta tgtaattcag
    22561 tatcacgccc atacaatccg aattttagtg agaattgtgg ctcagacagc caaatcagcc
    22621 tctttcgctt gtcgcttttc actaaacatc cgacaattta gcctaacccc ggcaaaaatg
    22681 gagatgccta tgtccacgac atggtttgta ggagccgact ggctcgccga acatattgat
    22741 gacccggaaa ttcagattat cgatgcccgc atggcgtcgc ctggacagga agatcgtaac
    22801 gttgctcagg agtatctgaa tggacatatt cccggcgcag tgttttttga tatcgaagcg
    22861 ctttctgatc acacttcccc gcttccgcac atgctgccgc gcccggaaac gttcgccgtg
    22921 gcgatgcgtg aattaggcgt taaccaggat aagcacctga ttgtctatga cgaaggtaat
    22981 cttttctcag ccccacgagc atggtggatg ctgcgcacct ttggtgtaga gaaagtgtcg
    23041 attctggggg gtggacttgc aggctggcag cgcgatgatc tgctgttaga agaaggtgca
    23101 gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc ctgaagccgt ggtgaaagta
    23161 accgatgtat tattggcaag ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct
    23221 gcacgtttta acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg acatattccc
    23281 ggagcactga atgttccgtg gacggaactg gtgcgcgaag gtgaactaaa aacgaccgat
    23341 gaactggatg cgatattttt tggtcgcggc gtcagctacg acaaaccaat tatcgtcagc
    23401 tgcggctctg gtgtaacggc agccgtggtt ttgttagcac tcgcgacgct ggatgtgcca
    23461 aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg cgcgggcaga tttaccggtt
    23521 gagccagtga aataagtatt ttacaggcaa taaaaaaccg ccgaatttgg cggtttttta
    23581 ttgctggtct ggttcgcggc ctttccagca ggttgtatta ccgtagtaat gcaagcgcgt
    23641 ctcagcggag acaatactcg ccagtaactc tctttttgtc aagcaaaaga gagttattat
    23701 tgttctgtta gtgtattatc cactgcggcc ctttccgccg tctcgcaagc gggcgctggc
    23761 ttaaggaaag gatgttccgc gaccgttaga gcaagcgcgt ctcagcgctt gctatcgcgg
    23821 caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca atgaagatac
    23881 ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt ttcccaagta aataataacc
    23941 ggagtctctc cggcgacaat ttactggtgg ttaacaacct tcagagcagc aagtaagccc
    24001 gaatgccgcc ctttagggcg gcatatttta gattatccgg ttttgtttaa agtcacgcaa
    24061 aaaaccaccc cagcgacgtt catagaatgg cacaatatgt tcggtaataa agtggctaat
    24121 tcctttttcc ccttttttca cctgacaaat atcgattggt tcatcgccag gtaatgtatc
    24181 ggtcgctaca cttcccgtcg cctgaataat ttcttcgata tcaccatcgg cttcaatgcc
    24241 aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt gaacaaataa aagcacgctt
    24301 caccggctta atggttttaa ataaggtggt gagtgaatca atcatttgtg ctggcggctc
    24361 tgcgacttcc gataatatca gcgattcacc gccttccagg atttcctggc tgctcagcgg
    24421 atttccctct tcaccaatca acaaactgat ttcacgcggc ataaattctt taccggtcgg
    24481 cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc tcaaacaatg tgcgaacggg
    24541 cattacgaca aatgcctgtt cgtcttcaac cgcctgttga agcgcttcta acgaggtgaa
    24601 aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc tgtaaatcaa gcgcactatc
    24661 ttcaaccaca gcctcgccct gcgccgccgt accaggcacc cagacggtgg attccagtag
    24721 agtacggaaa aaggccgggc ggtgcgccgg ttcggttgct gctttttcca gcaggtcttc
    24781 aagttcgttt tttgtttcgg acataagaac cacaattcat tcaacgttcg gggcgcaaat
    24841 gcctgatgcg ctacgcttat caggcctaca agggattcgc aatttgttga atttgcagaa
    24901 tttgtaggcc ggataaggcg tttacgccgc atccggcata ttagtttacg ccgttaacag
    24961 attagctatc gtgcgcacac caagtcccgt agcgcccgca gaccactgtt caaccggcgc
    25021 tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt tctcaacaaa
    25081 gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc ttccggtatt
    25141 gttcagttcg gcaaagttag acggcagctg gctgcggtgg aattccgcca gcggcagacg
    25201 ccagaatggt tcgttctctt gtgaggcact cgccagcaaa cgaccggcaa gcgcatcgtc
    25261 aaaactgaac agcgcgtgat aatcattacc cagcgcagtt ttcgccgccc cggtgagggt
    25321 cgccgcatca atgatcattt ccggtttctg cgcactggca tcaatcagac catcggcaag
    25381 caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt tgcgataggt
    25441 gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac agcagaggaa
    25501 cagcttcacg cgcttgttca gtccgcgcgt aatggcaaat gccagcgccc cggtaaccgt
    25561 tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt tgatgctgta
    25621 gccgccggag tcaaaagtga tacctttacc taccaggcac gcgtacactg gcgcttcttt
    25681 atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg aaccgcgtcc
    25741 gacggtgtgc agccccatat aaccttgctc acgcagatct tcgcctttgg tgatccgata
    25801 agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg ccagttgcga
    25861 tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga tgatcatcag
    25921 gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca ctttacgcgt
    25981 gcctttcggg gctttgtaac cttgccagaa tgcccagcag cgatccgcat cccagccttc
    26041 accgcttaac tgaacatgct tgatgcccag accgtcaatc ttgcgcgccg cacgctggat
    26101 cagccccaga tcgtctgccc cgttcaaatg cagggtaatg ccgtcattat taatgctgta
    26161 agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa tcttcatcgc
    26221 ttctgtcatt ttagttatcc ttctttgtaa aagggccgcc agttggcagc cccaggattt
    26281 ttccgctaaa tgattcgcgt tgcagaaagg cggcaagtga gtgaagcccc aggagcatag
    26341 ataactatgt gactggggtg aacgagcgca gccaacgcat ctgcggcgtg aagcatgacg
    26401 cggaaattat tcggcctcgt ccagccagac taacaaaatc gcttcgagaa ttttctcgtt
    26461 ggatgcctgc gggtcgtcgt cgaaatcttc cagatcgcaa atccactgat gcatatcggt
    26521 gaatcgaacc gttttcggat caagatcagg atacgcatcg tacagtgctt cgccaatttc
    26581 gcggctatcg gtccacttaa gtcccatact aacctctgtt aatgctcacg cgcatggttg
    26641 atagtgtaac gcgggatttc gactactaaa tcttcgtcgg taacacgtgc ctggcagctt
    26701 aaacggcttt cgggctccag tccccaggct ttgtccagca tgtcgtcttc ctgctctgag
    26761 ctttctggca gcgagtcaaa accttcacga acgatgcagt ggcaggtggt gcaagcacag
    26821 gatttttcac aggcgtgttc aatctcgata ccgttacgca gagctgcgtc gagaatggtt
    26881 tcaccgctat tagcttccag aacagcgcca tcaggacaga gatcctgatg aggcaaaata
    26941 acaatctttg gcatattaaa cctcgtccac ggaatggcct ttcagcgcac gacgaaccga
    27001 ctggtccatg cggcgagcgg cgaaatcctg ggtttgtttg tctacgtttt taatcgcttg
    27061 ttcgatggcg tcaacgtcat cgccctgcgc cacttcactc aggtgagcgg cagcatcgtc
    27121 aatgacctga cgttctgcgg cgcttaacag cgcggcatca gcagccagcg cgccgtgcag
    27181 actttccagc acacgcgccg cttctacttt ttgttctgcc agcattcggg cttttacgtc
    27241 ctgctcggca tagctcattg agtctttgat catcgaagcg atttcgctat cggtcagacc
    27301 gtaagacggt ttgacctgaa tagacgcctc aacgccggtg gatttctcca tcgccgtcac
    27361 gctcaaaaga ccgtcggcat cgacctggaa cgtcacgcga atatgcgcac caccagccgg
    27421 tagcgccgga ataccacgca gcgcaaaacg cgccagtgag cggcagtcct gcaccagttc
    27481 gcgctcaccc tgcattacat ggatagacat cgccgtctga ccatctttaa aggtggtgaa
    27541 atcctgagcg cgggccaccg gaatagtggt attacgcgga atcactttct ccaccaggcc
    27601 gcccatcgtt tcgaggccca gcgacagtgg gatcacatca agcagcagca tttcgctgtc
    27661 tggcttgtta cccaccagaa tatccgcctg aatcgccgcg ccaatagcga cgactttatc
    27721 cgggtcgatg gaagtcagcg gtggacgacc gaaaaattcg cctacccgtt cacgcaccag
    27781 cggcacgcga gtagaaccgc ccaccatcac cacttccagc acttcgtcag cttctacacc
    27841 cgcgtcttta agcgcgcgac gacaagccag taaggttcgt tttaccagtg gcgcgatcag
    27901 ttcattgaat tgttcacggc tgatttcgcc ctgccagccc gcaacgttaa cggtcacgga
    27961 gtccgcatcg ctcagcgcga ttttggctgc aatggcggca tccagcagtt cacgctgaac
    28021 gcggttatcg ctacgatcag gaatgcccgc ctgctcgcga atgtaatccg ccagcagatg
    28081 gtcgaaatca tcgccgccga gcgcggaatc accgccggtt gccagcactt caaacacgcc
    28141 gcgacttaag cgcagaatgg aaatatcaaa cgtcccgcca ccgaggtcat aaacagcgat
    28201 cacgccttcc tgaccggaat ccagcccgta ggcgatagcc gcagcggtcg gttcgttaag
    28261 taagcgcaga acatgcaacc ccgccagacg cgccgcgtct ttggtgccct gacgctgggc
    28321 atcgtcaaag tacgccggaa cggtgataac tacaccatcc agctcgcctg ccagggcttc
    28381 agttgcccgc gccgccagtg ctttgaggat gtccgcagaa acgcgcaccg ggttcagcag
    28441 ccccgccgcc gtttcaatca tcggcaggcc gttttcgctg gcctggaatt gataaggcag
    28501 atgcggatag cgttgctgga tatcagccag cgagcgcccc atcaggcgtt taacagaact
    28561 aatggtgttg gcggtgtcaa gcgctgcgtt agtacgcgcg tcataaccca ccgaatgccc
    28621 ttgctgttga tagtgaacaa cagatggcag caggtgacgg ccttcatggt cggctaacgt
    28681 ttcggcctga ccgctgcgca ctgtcgccac cagcgagttg gttgtgccca ggtcaatacc
    28741 agccgccaga cgacgctgat gcggcgcagc actcaaacca ggttcactaa tttgtaataa
    28801 ggccatgttt agcttccaga aattaaaaat cgagcagttt ttcttcgagt tgttcggcac
    28861 tgctccgcag tttatcgaga aaacgcagct tacgcacggt atccgccgcc gcgtcccacg
    28921 cctcgttgtc taactgttca accatcaact gatggcgggt atcaaacatc tttttcacac
    28981 gtttgataaa gctttccagc cgcgcttcat ctttcgcctg ttcgatctcg tccagctctt
    29041 cgcgcagctc caactgttcc atcaggaacg cggtgtcgcg cacagtatgc tgctcgctgg
    29101 cgagatcaaa accgtgtaaa gaaagcaaat attccgcgcg cattaacgga tgacgcagcg
    29161 tttgccaggc ctggttaatg gttgcagatt gctgtacggc ggcgagttgt tccgcctggc
    29221 ttccgctggc gaatttatca ggatgatact gacgttgtag atcctgaaaa cgcaggctca
    29281 gcgcctgggt atcgagttga taacgggcag gcaagccaaa gaaggtgaag taatccataa
    29341 cattctcagg gctgcgatcc gccgcgataa cggcgaatca ggtaaaacaa accccacgcg
    29401 caggcgacca cggtggggtt atcggtatgc gcatcaaacg tggaagcttt cgccgcaacc
    29461 acactcatct ttgacgttcg ggttggtgaa tttaaaccct tcgttcaggc cttcttttac
    29521 gaagtccagc tgcgtaccgt ccagaaattg caggcttttg ccatcgacca cgactttcac
    29581 gcctttgtct tcaaacacga tgtcttccgg cgtcggttcg tcaacaaatt ccagtacata
    29641 agccatacct gaacatccgg aggttctcac gcccagacgc aggccaaacc ctttgccgcg
    29701 gttagccaga aaggtattta ctcgcgctgc tgcactgtcg ctcagtgtaa tcgacataac
    29761 caaacctcaa ctcttatttt gcttcacgtt tgcttttata gtccgcaatg gcggctttga
    29821 tcgcgtcttc tgccagaata gaacagtgaa ttttcaccgg cggcagttca agttcttcag
    29881 caatatcggt atttttgatc gcctgcgctt cgtcgagaga cttccctttc acccattcgg
    29941 tgaccaggga gctggaagcg atagcagaac cgcagccgta agttttaaaa cgcgcgtctt
    30001 caatgatacc ttcatcgttg actttaatct gcaacttcat cacgtcgcca caggccggtg
    30061 cccccaccat gccgctgccg acgttctcgt cgttgttgtc aaaggaaccc acgttacgcg
    30121 gattctcgta atggtcgata actttttcgc tgtaagccat tataaattct cctgattccg
    30181 ataccgatta atgatgagcc cattcgatgc tgttcagatc cacgccctgc ttgtacattt
    30241 cccacagcgg agaaaggtca cgcagacgac cgatggattt acgaactaac tcgatggtgt
    30301 agtcgatctc ttcttcagta gtaaaacgac ctaaagagaa acggatagag ctatgtgcca
    30361 gctcgtcgtt cagccccagc gcgcgcagca cgtaggacgg ttcgaggctt gctgacgtac
    30421 aggcggaacc tgaagaaact gcgaggtctt tcagcgccat aatcagcgac tcaccttcaa
    30481 cgtagttgaa gctgacgttg agaatgttcg gcgcaccgtg ttccaggtca ccgttcaggt
    30541 aaacttcttc gatatctttg atgccgttcc acagacggtt acgcaggccg cgcagacgtt
    30601 ccatctcggt cgccatctct tcttttgcga tgcgataggc ctcgcccatt ccgacgatct
    30661 ggtgaacagg cagagtgccg gaacgcatac cgcgctcgtg accgccgccg tgcatttgcg
    30721 cttcgatgcg tacgcgcggt ttacgacgta catacagcgc accgatacct ttcgggccat
    30781 agattttgtg accggagaaa gacatcaggt caactttcaa ctggctcagg tcgataggca
    30841 gtttgcccac gctctgggtt gcatcaacgt gatagataat gccacgagca cggcacattt
    30901 cgccgatagc cgcgatatcc tgcaccacgc cgatttcgtt atttacgtgc atgatggaaa
    30961 caaggatggt gtcatcacgc atcgctgctt caagttcttt cagatcgata atgccgttac
    31021 gctgcggtgc caggtaggtg acttcaaaac cttcgcgctc cagctgacgg caggtatcca
    31081 gtaccgcttt gtgttcggtt ttgctggtga tgatgtgctt gccttttttc tgataaaagt
    31141 tggctgcacc tttgatcgcc aggttgtcag attcggttgc accagaggta aagacgattt
    31201 cacgcggatc agcgccgacc agatcggcaa tctgattacg ggcgatatct accgcttctt
    31261 cagcctgcca gccgaaacgg tgagaacggg aggccgggtt accaaaggtt ccgtccatcg
    31321 tcataaactg catcattttc tcggcaacac gcgggtccac cggcgtggtt gcggagtagt
    31381 cgagataaat cggtaatttc attgctctat aaactccgta catcactcaa tgcaaggaac
    31441 caggctaccg gctggatgta cgaccgtgtt tacggagcgc gatacgcccc ggcctgattc
    31501 taatatcttg ttatctttaa cctaactgtt tcgggtttca gcaagttgac aaacttacga
    31561 atctccggga gcttacataa gtaagtgacc ggagtgagta tgtgcagtca acgcagatgc
    31621 aacacgaaac aggacggtta aattaagcgc gtaacttaac gtcgatcgcg tcttgtatgc
    31681 gggtgcgtgg cgcgtcgtga gtatgctgac gaccagacac atccagcact tcctggttat
    31741 taaccagttc gcctaaagta atgttgttga gaaaaccggt gagacggtcg ctcaaatcac
    31801 gccacagcgc gtgggtcagg catttatcgc cgccctggca accgccttta ccctgacaac
    31861 gggtagcatc tacagattcg tcaacggcgc taatcacttc gccaacggcg atgctgctgg
    31921 catctttgcc taacagataa ccaccgcctg gtccacgtac gctggaaacc agaccatttt
    31981 tacgcagacg ggaaaacagt tgttccagat aagaaaggga aattccctga cgttcggaaa
    32041 tatcagccaa cggtaccggg cccgcttcag agttgagcgc aacgtcaagc attgcggtca
    32101 cggcatagcg ccctttagat gtcagtctca tgtcttactt cacctcaaac tcgcccctgc
    32161 ccggggtttt ttattgtaaa agtgggggta ttgcatagca gggtcaagtc tgacattccc
    32221 gagtaaattg gtcaactatt tacttgactg atttagtcgg gtatttaacc ttcagtgcca
    32281 tttttttatc gtggcgtttg tacctgttgt ccttccagac catgggcggg ttgagggcaa
    32341 ttattcgcat tagcgctggc ctcttcccct caccctaacc ctctccccga aggggcgagg
    32401 ggaccgtccg tgcatttatc tggcttagcc ctcaggcctt attaccttta ttctgctgct
    32461 ctatagaagc cagaatcccg cgcaggatat tcaattcctg gctttccggg cgcgcacggg
    32521 taaacagacg gcgcaattta ttcatcacct gccccggatg gttttcacgg ataaaaccgg
    32581 ttgccagcag cgtttgttcc agatgaccat aaaaacgctc cagatcatcg accagcggat
    32641 acggcgtctc ttcatgctcg acctgctcgc cattttcctg agttgccagc caggccatac
    32701 gcacttcata ggcaataacc tgaaccgcca ttgccaagtt cagcgaactg tattccgggt
    32761 ttgccgcaat cgcgacatga taatggcatt tctgcaactc ttcattggtc aagccgacgc
    32821 gctcacgacc aaacaccagc gccaccgggg tatttgccgc ttcagcgacg cttttcagac
    32881 cgcattcgcg cgggtcgagc atcggccacg gcagcgtgcg ggagcgtgcg ctggtgccca
    32941 ccaccaggct acaaccggct aacgcttcgt cgagcgtatc gacgatgtga gcattaccaa
    33001 tcacatcgct ggcccctgct gccagggcaa tcgcctggga gtcgggtttc accagtggat
    33061 taaccagcca cagattggtt aatcccattg ttttcatggc acgggcaaca gaacccatat
    33121 tgccggtgtg tgacgtctcc accagcacaa ttcgaatatt ttgcagcatt gtctttcttc
    33181 atctaaagat tattcacgca tcttatcata aaacgaagac agatgccgat ctcgctgcta
    33241 tactctgcgc cgttttcccg ttctttaaca tccagtgaga gagaccgatg catccgatgc
    33301 tgaacatcgc cgtgcgcgca gcgcgcaagg cgggtaattt aattgccaaa aactatgaaa
    33361 ccccggacgc tgtagaagcg agccagaaag gcagtaacga tttcgtgacc aacgtagata
    33421 aagctgccga agcggtgatt atcgacacga ttcgtaaatc ttactcacag cacaccatca
    33481 tcaccgaaga aagcggtgaa cttgaaggta ctgatcagga tgttcaatgg gttatcgatc
    33541 cactggatgg cactaccaac tttatcaaac gtctgccgca cttcgcggta tctatcgccg
    33601 ttcgtatcaa aggccgcacc gaagttgctg tggtatacga tcctatgcgt aacgaactgt
    33661 tcaccgccac tcgcggtcag ggcgcacagc tgaacggcta ccgtctgcgc ggtagcaccg
    33721 ctcgcgatct cgacggtacc attctggcga ccggcttccc gttcaaagca aaacagtacg
    33781 ccactaccta catcaacatc gtcggcaaac tgttcaacga atgtgcagac ttccgtcgta
    33841 ccggttctgc ggcgctggat ctggcttacg tcgctgcggg tcgtgttgac ggtttctttg
    33901 aaatcggtct gcgtccgtgg gatttcgcgg caggcgagct gctggttcgt gaagcaggcg
    33961 gcatcgtcag cgacttcacc ggtggtcata actacatgct gaccggtaac atcgttgctg
    34021 gtaacccgcg cgttgtgaaa gccatgctgg cgaacatgcg tgacgagtta agcgacgctc
    34081 tgaagcgtta atgactcagg cgggtgatgt cactcacccg ccctcgcctt tcaggcgcta
    34141 ttctgaaata cttcctcacc gctttacttt ctttcgtcac tctcccacca tctttccccg
    34201 attaatggat gataaagaag tcacaaggaa aggttatggc gctgccagtg aacaaacgcg
    34261 ttctcaaaat tctgtttatt ctctttgttg ttgccttctg cgtttattta gtgccgcgcg
    34321 ttgccatcaa cttcttctat tatcctgacg ataaaattta cggtcccgac ccctggtcgg
    34381 cggaatccgt cgaatttacg gctaaggacg gtactcgtct gcaaggctgg tttattcctt
    34441 cttcgacggg ccctgctgac aatgccatcg caaccatcat tcatgctcac ggcaatgccg
    34501 gaaatatgtc cgcccactgg ccgctggtca gttggttacc cgagcgtaat ttcaacgttt
    34561 ttatgtttga ttatcgcggg tttggtaaat caaaaggcac gccgtcccag gccggattgc
    34621 tggacgatac gcaaagtgcc atcaatgtgg tgcgccatcg cagtgatgta aacccacaac
    34681 ggctggtgct gttcgggcag agtattggcg gggcgaatat tcttgctgtt attggtcagg
    34741 atgatcgtga aggcatacgt gcggtgatcc tcgactccac atttgcctct tatgcaacca
    34801 tcgccaatca gatgatcccc ggcagtggct acttacttga tgagagttac agcggcgaaa
    34861 attatatcgc cagcgtcagc ccgatcccgc ttttactcat tcacggtaaa gctgatcacg
    34921 ttatcccatg gcagcacagc gaaaagttgt atagcctggc aaaagagaca aaacggttga
    34981 tcctaatccc ggatggcgaa cacattgatg ctttttccga tcgtcacggc gatgtttatc
    35041 gcgaacagat ggtggacttt atccttagcg cgttgaatcc gcagaactaa cccatgatcg
    35101 ctagcacgat aatcattcac aaaaccacct taagacatgc taatccactg gtcagaacag
    35161 tttaagatga gaaaaattct gtgacgcttg ccaacatttc tgatgattag cattcccttc
    35221 gccatttccc tgagcaaact ttagctattc ttatcaatta tgcttatggg agatctacag
    35281 atgatgccta cgcttgctcc accatctgtc ctttcggctc cccagcgccg ctgccagatc
    35341 ttgctgacgc tctttcagcc ggggttgacc gccaccatgg caaccttcag cgagcttaat
    35401 ggtgtggatg atgatattgc cagtcttgat atcagcgaaa caggacggga gatcctgcgc
    35461 tatcatcaac tcacactgac gactggttat gacggtagct accgggttga aggtacagtg
    35521 cttaaccaac gtttgtgttt atttcactgg ctacgacgtg gtttccgtct gtgtccgtca
    35581 tttattacca gccaattcac ccccgccctg aagagtgaac tgaagcggcg cggaattgcg
    35641 cgtaactttt acgacgatac caatctacaa gcgttagtga atctctgctc ccgacggctg
    35701 caaaaacgct ttgaaacgcg cgatattcat ttcctgtgtc tgtatctgca atattgtttg
    35761 ctgcaacacc acgctggaat tacgcctcag tttaatccgc tccaacgtcg ctgggcggaa
    35821 tcctgccttg aatttcaggt agcgcaggaa attggacgcc actggcagcg tcgggcgctc
    35881 cagcctgtac cgcctgatga gccactgttt atggcattac ttttttccat gttgcgggtt
    35941 cccgatccat tgcgtgatgc gcatcagcga gacagacaat tgcgtcagtc tatcaaacgt
    36001 ctggtaaacc attttcgtga gctgggaaat gttcgttttt atgatgaaca ggggttatgc
    36061 gatcagcttt atacccacct cgcccaggcg ttaaatcgca gtttgtttgc catcggtatt
    36121 gataataccc tgccggaaga gttcgccaga ctgtacccac gcctggtgcg caccacccgc
    36181 gcggcgctgg ccggatttga aagtgaatac ggcgtccatc tttctgatga ggaaagcggt
    36241 ctggtcgcgg tgattttcgg tgcctggcta atgcaggaaa acgatctgca tgaaaaacag
    36301 attattctac tgaccgggaa tgatagcgag cgagaagcgc agattgagca gcagttacgc
    36361 gaactaacgt tactgccgct caacattaag catatgtcgg taaaggcatt tttgcagaca
    36421 ggcgctccgc gtggcgcggc actgattatt gcgccttata ccatgccatt accgctcttt
    36481 tcaccaccgc tgatctatac ggacctgacg ctgacaacac atcaacagga gcagatccgc
    36541 aaaatgctcg aatcagcatg agggaacaac tttcgggcgc aaaaacattg ccggaagcgc
    36601 caccagcgcc attacccaga acacgccgtg gcccagatat tgatacagga aaccggcgaa
    36661 aacggtcatg atagcgatac tgccgcccat cgcgacggca gagtaaaccg cctgtaaacg
    36721 gatgacttcg ctaccctggc gaacagcaat atagcgcatg gcggccaggt ggcagaccgt
    36781 gaaggtgccg caatgcaaaa tttgcaccac tatcaaccac ggcaacgccg tagttgctcc
    36841 cataatgccc cagcgcacta cgccgcaaat cgccgagatc aacagcatat cgcgagcact
    36901 acaacggcgg aaaagtttat tactcagcgc aaagataatg acttccgcca ccacgcccag
    36961 cgaccacaaa taccccaccg ccgaggccga gtagccagct gcctgccagt aaatggcgct
    37021 aaaaccgtaa taggccgcat gtgccccctg caataaacaa acgcaggcca gaaagcgcca
    37081 gttctggcga accagcgcca accacgcaga ccaaccggtg ctctcctgct ggcgacttgc
    37141 cccttgtggc tgaatcgtcg gacggatgag aaagccgagc agcatggatg ccacgcccaa
    37201 cgtcaacagc gcgaggatca cccgataatc aaacatagtg accagtttgc ccgtcagcgc
    37261 cgagccaatg acaaacgcca ccgagcccca cagtcgcact ttgccgtaat caagcgggaa
    37321 ctgcttttgc cacgtattcg ccagtgcatc ggtcaacggt accagcggtg agaaaaagag
    37381 gttaaagcca atcatcacca gcatcagcca cgctacgtgc gcccccgccc agaaggcgac
    37441 agcaaagaga agtgtcagca gtgccagcac gcgcaaggcg gaaatcaggc gggaaggatc
    37501 gctgacgcgg ggcgcgatga gcaaactccc gagaaaacgg gcaaccagac ctgcccccaa
    37561 taacaggccg atggtttctg gcgttaaacc aatcccttta agccagacgc tccagaaagg
    37621 tagaaaaatg ccgtaactaa aaaagtatgt gaaatagccg agcgccaacc agcgcgtgga
    37681 ttgcaaaacc atgagtccct cccgtcagga ggcgttagtc tggggttaat cgaatgttgg
    37741 tgcaagttga aaaataagaa tattcgagcc tgcttcccga tgtctgtaac gccagttcac
    37801 aagcactaaa aaactgcgat ccgcgacgca tgttgcaaaa ataatgcgta attatgccgt
    37861 tacgcttgcc aaacgttcct gcacaatggc gatgaaatcg cgtaatgccg gcttcatctc
    37921 ccctttcttc cacgccatca gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa
    37981 aacaacctgc ccggtattaa aattattcat ataaccgggt atcaaagtga cgcccagccc
    38041 catgcccacc agattcatgg tcaccagaat attcgttgcc acctggacga tatttggctg
    38101 gctgttttct tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg aatacgccgg
    38161 atcggtactg acgaaattca cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc
    38221 taaagggtga tcaaccggta acaccaccac taatggttcg tcaaaaagct ccagataatc
    38281 aatctccggg ctataaacag gatggcgcat taagccgacg tcgagttcac cacggcgaat
    38341 tttttcctcc tgttgcgtgg tgattaaact caccagctca atcaaggtgt ctggctgtct
    38401 gagacgaaac attggtaata cttttggcaa taaattcact tccgccgatg gcacaaagcc
    38461 aatggttaat tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg cattttccgc
    38521 ttgttcgagg attgccagcg cgtcctggag aaaacattct cccgccgccg tcaacgcgac
    38581 tttgcgctta tccctcacca gtaacggaac accgacacag ttttcaagat cgcggatctg
    38641 gctgcttaac gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa agttcagtgc
    38701 ctgcgccact gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc
    38761 gaaaaaacgt ctcagctggt agaaattaac tatttcacat attagcaacc aaccaacaac
    38821 atccttatga cacaaaaata gaaggtcaat acatcttatc tttcaggatt aaaaaatatg
    38881 accacaccct cagatttaaa catttaccaa ctgattgata cccaaaatgg tcgggtcact
    38941 ccgcgtattt ataccgaccc agatatttac caactggagc ttgaacgtat tttcggccgt
    39001 tgctggttat ttctcgccca cgaaagccag atcccaaaac ctggtgattt ctttaacacc
    39061 tacatggggg aagatgcggt tgtcgtagtg cgtcagaaag acggcagcat taaggcgttt
    39121 ctcaaccaat gccgccaccg ggccatgcgt gtgagttatg cagattgcgg caacactcgc
    39181 gcctttacct gtccgtatca cggctggtct tatggcatta acggcgagtt gatcgatgta
    39241 ccgctggaac ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg actaaacgaa
    39301 gttccttgtg tggagagtta taaagggctg atttttggca actgggatac cagcgcaccg
    39361 ggcctgcgtg attacctggg tgacattgcc tggtatctgg atggcatgct ggatcgtcgc
    39421 gaaggcggca ccgaaattgt cggcggcgta caaaagtggg tgatcaactg taactggaaa
    39481 ttcccggcag agcagttcgc cagtgaccag tatcatgctc tgttcagcca tgcttctgcc
    39541 gttcaggtat taggggcgaa agatgatggc agcgataagc gcctcggtga tggacaaacc
    39601 gcccgcccgg tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga cggtcacggt
    39661 agcggtttct tctttactga aaaaccggat gctaatgtct gggtcgatgg cgcagtttca
    39721 agctattacc gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg
    39781 cgcctggcgg gtcataacaa tattttcccc acgctttcat ggctcaacgg cactgccacg
    39841 ctccgcgtct ggcatccgcg cggccctgat caagttgaag tgtgggcgtt ctgtattact
    39901 gacaaagccg cctccgatga agttaaagcc gcttttgaaa acagcgccac tcgtgctttt
    39961 ggtcctgctg gttttctcga gcaggatgac tcggagaact ggtgtgaaat ccagaaattg
    40021 cttaaaggcc accgcgcccg caacagcaaa ctgtgtctgg aaatggggct tggtcaggaa
    40081 aagcgccgcg acgacggcat tcctggcatt actaactata tcttttcaga aactgccgct
    40141 cgtggaatgt accaacgctg ggccgatctt ctgagtagcg aaagctggca ggaagtgctc
    40201 gataaaaccg ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt tcactagagt
    40261 tacatcaccg cattagccag tttctctttc acgaagccag cttactggac gactggaaat
    40321 ttcgtgactg gctggagcag ctcgacaaag agattcgtta caccatgcgc accacagtta
    40381 acgcgcaaac acgcgaccgc cgcaaaggcg tccagccacc gacaacctgg atttttaatg
    40441 acaccaaaga ccagctggag cggcgaatcg cccgtctgga aacgggcatg gcctgggcag
    40501 aagagccgcc gtcacgcacc cgtcacttaa tcagcaactg ccagataagc gaaaccgaca
    40561 tcccaaacgt atttgctgtg cgggtaaatt atctgcttta tcgggcacaa aaagagcgcg
    40621 atgaaacatt ctatgttgga acgcgtttcg acaaagttcg ccgtctggaa gatgacaact
    40681 ggcgcttgct ggaacgggat atcgtcctgg atcaagcggt aatcacttcc cataacctga
    40741 gtgtactgtt ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga
    40801 ggctctccgg atcgatacct cgcccgtcat cgccctgttc aacgttggcg gcgagtttta
    40861 tgccattaac gatcgttgca gccatggtaa tgcgtcgatg tcagaagggt atctggaaga
    40921 tgacgccacg gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc
    40981 gttatgcctg cccgccaccg atccgctcac cacttatcca gtacacgttg aaggtggtga
    41041 cattttcatc gacttaccgg aggcgcagcc atgagcgatc tgcataacga gtccattttt
    41101 attaccggcg gcggatcggg attagggctg gcgctggtcg agcgatttat cgaagaaggc
    41161 gcgcaagttg ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt
    41221 ggcgaacata ttctggcggt ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg
    41281 gtcgatcaga tcctgactcg ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc
    41341 tgggatcaca atgcctcact ggttaatact cccgcagaga cgctcgaaac cggcttccac
    41401 gagctgttta acgtcaacgt actcggttac ctgctgggcg caaaagcctg cgctccggcg
    41461 ttaatcgcca gtgaaggcag catgattttc acactgtcaa atgccgccag gtatcctggc
    41521 ggcggtggcc cgctgtacac cgccagtaaa catgccgcaa ccggacttat tcgccaactg
    41581 gcttatgaac tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc
    41641 gacctgcgcg gcccacaggc gctcgggcaa agtgaaacct cgataatgca gtctctgacg
    41701 ccggagaaaa ttgccgccat tttaccgctg caatttttcc cgcaaccggc ggattttacg
    41761 gggccgtatg tgatgttggc atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc
    41821 aacgctgatg cgggtttagc gattcgcggc attcgccacg tagcggctgg gctggatctt
    41881 taaggaagca cgatgaaaga aaaaacgatc attattgtcg gtggcgggca agcggcggca
    41941 atggctgcgg cctcgctacg ccagcaaggg ttcaccggtg agctgcatct gttttccgat
    42001 gagcgacatc ttccttatga acgacctcca ctctcgaaat ccatgttgct ggaagattcc
    42061 ccgcagttac agcaggtgtt acccgctaac tggtggcagg aaaacaatgt tcatctgcat
    42121 tccggtgtaa ccatcaaaac gctgggtcgc gacacacgag agttagtgtt aaccaacggc
    42181 gaaagctggc actgggatca gctttttata gcaatcggcg cggcagctcg accgctgccg
    42241 ttgcttgatg cactgggaga acgctgcttt accctacgcc atgccggtga tgccgccaga
    42301 ctgcgagaag ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg aactattggt
    42361 ctggaactgg ctgccagcgc cacgcagcgc agatgtaagg tgacagtgat tgaactggcg
    42421 gcaaccgtca tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccac
    42481 cagcaggctg gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa
    42541 aaagtagaac tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt
    42601 attggtatca gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc
    42661 attgtcattg atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg
    42721 gcaatcactc gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat
    42781 aaccaggcgc aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg
    42841 ccgtggttct ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc
    42901 gatgactggc tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa
    42961 aacggcgtgc ttatcggtgc ggtcacgctg aatcaggggc gtgagattcg cccaattcgc
    43021 aaatggatcc agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg
    43081 cttaaatcac tgtaaccagg ataattagcg aatatctcaa cgcctggggc gtggcgaggt
    43141 gcaagagtgt gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca
    43201 cta
//
LOCUS       DAZOZD010000008        40456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOZD010000008 DAZOZD010000000
VERSION     DAZOZD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.008"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            113..754
                     /locus_tag="ACHUPB_000334"
     CDS             113..754
                     /locus_tag="ACHUPB_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0757254.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            895..1557
                     /locus_tag="ACHUPB_000335"
     CDS             895..1557
                     /locus_tag="ACHUPB_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFR0757255.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            1824..2444
                     /locus_tag="ACHUPB_000336"
     CDS             1824..2444
                     /locus_tag="ACHUPB_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0757256.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLTP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSCWLGELNPWPDVL"
     gene            2471..4165
                     /locus_tag="ACHUPB_000337"
     CDS             2471..4165
                     /locus_tag="ACHUPB_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0757257.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            4246..4488
                     /locus_tag="ACHUPB_000338"
     CDS             4246..4488
                     /locus_tag="ACHUPB_000338"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0757258.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            4604..4939
                     /locus_tag="ACHUPB_000339"
     CDS             4604..4939
                     /locus_tag="ACHUPB_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757259.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            5166..6236
                     /locus_tag="ACHUPB_000340"
     CDS             5166..6236
                     /locus_tag="ACHUPB_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR0757260.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            6240..6677
                     /gene="pilM"
                     /locus_tag="ACHUPB_000341"
     CDS             6240..6677
                     /gene="pilM"
                     /locus_tag="ACHUPB_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR0757261.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            6709..8328
                     /locus_tag="ACHUPB_000342"
     CDS             6709..8328
                     /locus_tag="ACHUPB_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR0757262.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            8349..9644
                     /gene="pilO2"
                     /locus_tag="ACHUPB_000343"
     CDS             8349..9644
                     /gene="pilO2"
                     /locus_tag="ACHUPB_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR0757263.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            9634..10092
                     /gene="pilP"
                     /locus_tag="ACHUPB_000344"
     CDS             9634..10092
                     /gene="pilP"
                     /locus_tag="ACHUPB_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR0757264.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            10196..11704
                     /locus_tag="ACHUPB_000345"
     CDS             10196..11704
                     /locus_tag="ACHUPB_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR0757265.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            11706..12800
                     /locus_tag="ACHUPB_000346"
     CDS             11706..12800
                     /locus_tag="ACHUPB_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR0757266.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSELVIGYGLWLLAGLLLLAILIRMSFSLYKGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNSGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKSVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            12862..13398
                     /locus_tag="ACHUPB_000347"
     CDS             12862..13398
                     /locus_tag="ACHUPB_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR0757267.1"
                     /translation="MSVITCSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            13443..13928
                     /locus_tag="ACHUPB_000348"
     CDS             13443..13928
                     /locus_tag="ACHUPB_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0757268.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            13944..14570
                     /locus_tag="ACHUPB_000349"
     CDS             13944..14570
                     /locus_tag="ACHUPB_000349"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0757269.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            14588..15949
                     /gene="pilV"
                     /locus_tag="ACHUPB_000350"
     CDS             14588..15949
                     /gene="pilV"
                     /locus_tag="ACHUPB_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR0757270.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            16063..16257
                     /locus_tag="ACHUPB_000351"
                     /pseudo
     CDS             16063..16257
                     /locus_tag="ACHUPB_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            16422..17243
                     /locus_tag="ACHUPB_000352"
     CDS             16422..17243
                     /locus_tag="ACHUPB_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR0757271.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            17345..18547
                     /locus_tag="ACHUPB_000353"
     CDS             17345..18547
                     /locus_tag="ACHUPB_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR0757272.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            18651..19109
                     /locus_tag="ACHUPB_000354"
     CDS             18651..19109
                     /locus_tag="ACHUPB_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFR0757273.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            19106..19942
                     /locus_tag="ACHUPB_000355"
     CDS             19106..19942
                     /locus_tag="ACHUPB_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR0757274.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            19926..21074
                     /gene="traJ"
                     /locus_tag="ACHUPB_000356"
     CDS             19926..21074
                     /gene="traJ"
                     /locus_tag="ACHUPB_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR0757275.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            21071..21361
                     /gene="icmT"
                     /locus_tag="ACHUPB_000357"
     CDS             21071..21361
                     /gene="icmT"
                     /locus_tag="ACHUPB_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFR0757276.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            21425..25486
                     /locus_tag="ACHUPB_000358"
     CDS             21425..25486
                     /locus_tag="ACHUPB_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR0757277.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSEDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEHPAAPSSAPEKAPVSAQPQQPDT
                     PVEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            25503..25853
                     /locus_tag="ACHUPB_000359"
     CDS             25503..25853
                     /locus_tag="ACHUPB_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0757278.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            25865..26560
                     /locus_tag="ACHUPB_000360"
     CDS             25865..26560
                     /locus_tag="ACHUPB_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR0757279.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            26571..27545
                     /locus_tag="ACHUPB_000361"
     CDS             26571..27545
                     /locus_tag="ACHUPB_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFR0757280.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     QGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            27549..28886
                     /gene="traO"
                     /locus_tag="ACHUPB_000362"
     CDS             27549..28886
                     /gene="traO"
                     /locus_tag="ACHUPB_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR0757281.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPPGTVTSQHPRLQETSLATPAARPGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            28883..29596
                     /gene="traP"
                     /locus_tag="ACHUPB_000363"
     CDS             28883..29596
                     /gene="traP"
                     /locus_tag="ACHUPB_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFR0757282.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGSGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            29593..30123
                     /gene="traQ"
                     /locus_tag="ACHUPB_000364"
     CDS             29593..30123
                     /gene="traQ"
                     /locus_tag="ACHUPB_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR0757283.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            30170..30568
                     /locus_tag="ACHUPB_000365"
     CDS             30170..30568
                     /locus_tag="ACHUPB_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFR0757284.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            30625..30876
                     /locus_tag="ACHUPB_000366"
     CDS             30625..30876
                     /locus_tag="ACHUPB_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFR0757285.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            30791..31609
                     /locus_tag="ACHUPB_000367"
     CDS             30791..31609
                     /locus_tag="ACHUPB_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFR0757286.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            31904..34948
                     /locus_tag="ACHUPB_000368"
     CDS             31904..34948
                     /locus_tag="ACHUPB_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0757287.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            34948..35568
                     /locus_tag="ACHUPB_000369"
     CDS             34948..35568
                     /locus_tag="ACHUPB_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFR0757288.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            35526..36731
                     /gene="traW"
                     /locus_tag="ACHUPB_000370"
     CDS             35526..36731
                     /gene="traW"
                     /locus_tag="ACHUPB_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFR0757289.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            36728..37297
                     /gene="traX"
                     /locus_tag="ACHUPB_000371"
     CDS             36728..37297
                     /gene="traX"
                     /locus_tag="ACHUPB_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFR0757290.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            37372..39537
                     /locus_tag="ACHUPB_000372"
     CDS             37372..39537
                     /locus_tag="ACHUPB_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR0757291.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTATSASEYHASKTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWITGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            39625..40278
                     /gene="excA"
                     /locus_tag="ACHUPB_000373"
     CDS             39625..40278
                     /gene="excA"
                     /locus_tag="ACHUPB_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR0757292.1"
                     /translation="MNTTKSRYPSWWAYYYLIRTAYFIFGVPFLLLCSLGFTGMMLTE
                     PYIETDIQAYLYTYGSWLLLILPSLWLYMKTRAKKKKVQQVVQQIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
BASE COUNT         9861 a        11527 c        10553 g         8515 t
ORIGIN      
        1 cacagataca tccttatgta atatattttc cagccattaa tcctgtttgc aggagttgtc
       61 ggttttcgcc cgcctcgaaa ccgtccacaa ggcttacaag agtgcgtaaa tcatgtgcct
      121 gtcagaactg caggccaggc tgaacgtggg tcagccgctg tttccgacac atgctttatg
      181 tattcgccgt tcacaacaac atcgaagccc ccctgcgggg atcgattttc gcggagcgtt
      241 ccggattttg aatatcagtg aattacatca acgcaactgg tatctggcac agtacatccc
      301 tgccggcaaa aaccgcgaac atctcttttc ctggctttcc gaacaacacg tccttccctg
      361 gacacccctg atactgaaaa aggtccgccg cacggataaa gtctgtggtt accgtcgaca
      421 tatacacgcc gtgtttcccg gttacttttt cctgaaagca gaccccgaaa gccacagctt
      481 cactcacctt cgccgtcaca gtgcctttct ggacttcgtg aaaatggccg gtgaaatcaa
      541 aaccgtcagg gaggatatcg ttcagtcttt gatgaaagtg tacccggatc ctgcgctcaa
      601 tccggcagcc agggaggagc tggatgctgc ctccaccctc tggctgacaa aagcgcgtta
      661 ccagtacctc ctccggcttg atgcacagcc tctgccggag tcgaggatcg ccctgctcct
      721 gcacctggta tctgatgacg gagcgttgac atgaaaattg ctgtcttaat ttgacgactt
      781 ttcccggcct ttatggccgg gatttttttc ccttctgttt ccacaatgcc cggcaccggt
      841 gacacctgag ggtgtacgga gagcggctgt cgggcatttt ttcaggacgc ctttatgacc
      901 attctatccc aggaaaaccc tgtcattctg ttactggaga atatgctcga agcactctcc
      961 accgccccgg acaacataaa caacgaacgg cgtcgccggc gttacctgct gaactggctt
     1021 gacactgccc gtcagatgcg cgagttcaga ggaatggctg aagagttcac taccctgcga
     1081 aaactgctgg ccaccaattc agacataccg gtagcaaaca ccctgaaatc actgctggaa
     1141 agtggcaata acgcagcatt atacgacctg taccgcttcc gctcggctct tgccacgtgc
     1201 aggagtctgg gatggcagat tggcacgtgc gcatggaatg accagttact gagtgagaca
     1261 ctttcccgcg cacagaccgg gaaacgtcat atcctgcagc tgaacagcac cggggacacc
     1321 ttttccacca cgggcagaat gatcagaccg gtggcatttt cattaatcca tccggccacc
     1381 atcaacacgt ctgaggttga agagatattc cgggatgaag gttttatgct cgcacacggg
     1441 cgcgaatgca gtctgaacgg cagcggcaga aatatgctgt cacgcattct tcatgtgggc
     1501 cattcaggtt tagcggaaga ggaatggggc atggacaaca gtctgctttt acactgaaat
     1561 accgggacag ccacaacggc agccactctc cgctgacttt ttttcgcaaa tcgcggtttg
     1621 ctgatgtgga tgtcccattt ttgattatcc gtcctctccg gttgtcataa agcgcattcc
     1681 ggcgtgaggc ttctgttgtt atcatcccct cctgcagaca tccccttgtc ttctgatgac
     1741 tgacgatatg cttcttctga caattaaaga agcggaagct tcacgccata atgttgtttg
     1801 actgacctgc cacaggagga aaaatgacac tgacgttact taccgcgtat aacgcaccct
     1861 atctggcagc cctgactttt gttctgctta ctggtatcgc tgaacttatc gccctgctct
     1921 gcggacactc cctctcttca gctatggata cacctgattt accggagggt ctgacaggag
     1981 aggcgctgga ctggctgaac atcggacgga tccccctgct gatcgtcctc tgtatgctgg
     2041 caggtttttt tggtatatcc ggcattctcc tgcagggtct tattattcat ctgctgcagg
     2101 ctcctgcccc gaacattctc cttacaccac tttgcctgct gctgacatgt cctctcgtac
     2161 accgcaccgg cagactgatt gccccctggt taccccgtga tgaaacaacg gcgctcaccg
     2221 aggaggcatt catcggctgt atggccacca tcaccggtta tcacgcagtg gcgggttccc
     2281 cctgtgaagg tcgcttcaca gaccgcttcg ggcaggttca ttacctgttg ctggagccgg
     2341 aggccgggaa agaattcaga cgaggagaca aagtgattat tgtctgccgt ctttccggtt
     2401 cctgctggct gggcgaactg aatccctggc cggatgttct ttaatcccga catatacaga
     2461 ggttctcact atgtttaatc ttccgggatg gttgtttacc gttatagcgc tggtcctcac
     2521 cctgttcgtt atcgggctga tatttgcccg cctgtaccgc cgggcatcgg cagagcaggc
     2581 ctttgtccgc acaggtctgg gaggccaaaa agtggtaatg agtggcggtg caattgttat
     2641 gcccattttt cacgaaacaa ttcccgtcaa catgaacacc ctgaagctgg aagtcagtcg
     2701 tgcagcggct gaaagtctca taacccgcga ccgcatgcgt gttgatgttg ccgttgcctt
     2761 ttttcttcgg gtaaaaccat cagccgaagg aatatccact gcagcccaga cgctgggaca
     2821 acgcacgctc acacctgaag atctgcgtag tctggtcgag gataaatttg ttgatgccct
     2881 acgggccacg gcggccagaa tgagcatgca ggatttacag gatgcccgcg agaactttgt
     2941 tcagggtgtg cagaacaccg ttgcggaaga cttatccaaa aacggtctgg agcttgaaag
     3001 tgtgtcgctg acaagtttca accagactgc acgggtacat tttaatcctg acaatgcctt
     3061 tgatgctgaa ggcctgaccc ttctgaccca ggaaacagaa cgtcgtcgcc gcgaacgcaa
     3121 cgaagttgag caggatgttg aagtggccat cagggaaaaa aaccgggatg cactctcccg
     3181 acgtctggag attgaacagc aggaagcctt catgactctg gagcaacaac aacgggtaaa
     3241 aacccgtacg gcagagcaga gtgccagtat tgctgctatt gaagctgaac gacgtcgtga
     3301 agcagaatcc gcgcgaattc tggctgaacg taagatagag gaagccgaaa tcgaacgaca
     3361 gcagatagtg cgtacccgcc aggtcgaagc cgaacgggaa gtggccatac gggaaattga
     3421 gcaacagcag gcaaccgaaa tcgccagcca ggccagagcc attgccgtgg cggcaaaatc
     3481 ggaagagcag tcacaggcag aagcccgggc aagtaaagca ctggctgagg ccgtacaggc
     3541 ccagcaggat gtggaaacca cccgccagac tgcggaggcc gaccgtacca aacaggttgc
     3601 cctgattgct gctgctcagg aagctgaaac gaaagcggtt gaactgacgg ttcgcgcaca
     3661 ggctgaaaaa gaagcagccg aactccaggc cgcagcaatt attgaactgg cggaggccac
     3721 ccgtcagaaa ggtctggcag aagcagaagc acagcgcgca ctgaacgatg ccattaacgt
     3781 gctttcggat gagcagacca gcctccgctt ccgtcttgcc ctgctggatg cattacctgc
     3841 cgtcatagag aagtccacag aaccaatgaa atccattgac ggtattaaaa tcattcaggt
     3901 ggatggtctg aatggcggtg ttacccgcgg taaaacagca acaaccgaca taagtggcgg
     3961 aaatctcgct gaacaggcag taaccgccgc tctggcttac cgtacacagg ctccgctcat
     4021 cgactccctg ctgaaagagg tgggtttatc cggtgaagga cttacagccc tgacacgtcc
     4081 actggtcatg cctgccccgg aaaaggttcc ggatgtcagc gaaccggtaa ccgctccgga
     4141 taactcagaa ccacaacagg tttaatcccc gtgccggaat ttttccggca tctttttcac
     4201 aaagaatgcc ctcacgggat attctggttt aaaagaggat tgaaaatgtc cgcagaaaaa
     4261 ttcagagttg tcatatacac caatacatgt gaaaagaaag tcagttatat tcctgcaaca
     4321 gaagatataa acatcgctat aatgccattt cagacggcag acaatggctt aattattgtg
     4381 tgtcagaact cccgtataat acgacgcaaa gagcgtattc ccggaagcag ctgtgaatgg
     4441 aaagatgtga cattagcacc aaaatccaga aaccgcgttc agttatagcc tgacagcctg
     4501 cttttcatcc cccataaaag ggggtaaaaa ttaaatattt cacaaccgcg atatttttca
     4561 ggaatgcaga ttattttctg ttatgaccaa ttgagaaaca ggcatgagaa caatcacaac
     4621 acgtgaacaa ttattaatta atgggagagt ccacgaaaga atcgccacac atatagttac
     4681 aggagctcat gggtatgaaa cgctttgcac aaatggctat aacgtcagat acaacaaggc
     4741 gcagcaactg gttgaaagct gcgaaaaaat agctgaaggt aaactacctg ttacctgtcc
     4801 tgtctgtttc accatatggc aggatgtaca cagattcacc cacgttgact ttgacacaca
     4861 atcggggaaa agtgatttta ttgatacctg ccattcggag atcataaccg gacatgtttc
     4921 aataacaggc aagaaataaa ataatgttcc agttctgaaa aacaatatca cttgttttct
     4981 ggaaactatc atttgcagca ggacgttttt ctttttatat ttccggaacc ggaatgtttt
     5041 agttttcata atcaccccga acatgaatgt tcatcatcaa tatcctgatg aaattatatt
     5101 tatctgaaga cctgttacca gccacctgtc ttataccgta acccgataca taccggattt
     5161 attctatgaa aaagaaatca cttacactcc ttctgccgtc attactcagc ggctgtacgg
     5221 catttaatgc cacgcctgtt cctgacacca cgcctccggc tctggtgttc gtcgacggac
     5281 aaattcgcga aagcgctgcc attatcgccc atacgcaaaa acgtctgctt ccgccaccgg
     5341 tgacccgccc tgtgacgccg ccccgcccga caacaacagc gacgcccaca accgcagtca
     5401 gcagccagag taccgcactg cctccccgtc cgctccttca ggccacacag ctgcccgcac
     5461 tggttatgac cggtaaaccc ggtcagtcat ccgtcgttta tttacgtgag gcacatcagc
     5521 gctccgtcag tgactggata cgccagttgc taccacccgg ctggcagttc cggcaacaga
     5581 acgctgccat accggcactg aacaaacgcc tccccgtctg gtccgttaat gaccagtggc
     5641 accgggcact gaatgcgctg ctggccggcc agcagcttta cggacatatg gactggaaca
     5701 ggaaaatact gactgtcacg acctctgcaa caccgccggt cgacgtcacg gcaccgcagg
     5761 gttcacagaa agctgctgat acaccgcgta atccgttcag gggcagcacc gcaacgccgg
     5821 caggcccgac accagtgaca cctgccgtca gcgtttcccg ggctcagacc accaccggga
     5881 catccggaac tccagagagc agaacaacca cgccagcccc ggtggccacg aaaccggtag
     5941 tgccaccaca gcagaaggtc ataccgccac ggcagtggaa aatggaaaag gggaccaccc
     6001 tgaaagacgg actggcccgc tggacatcat ccgaaaaatg cccgcacggc acatggacac
     6061 tgcgctggga aacacctgtc aactacagca tcgatgcccc tctggttttc accggcagtt
     6121 tccgccaggc gctggagcag ctctttgcac tctatacccc ggctgaaaaa ccgctgtatg
     6181 cccggaccag cactgcacaa tgcctgctcc gtgtgacaga ccgcccgcag gaataaccta
     6241 tgggatgggt tacgggtttc attatggtca ttatggccat tggtaatttc tggttccaca
     6301 gtcacctcag ccgtactgtg caccatcagc agaccgctga aatcacacag caggctgcag
     6361 actttattcg ttatatgaat gccatcaacg attacctgta tcagcaccct gaacgacgtg
     6421 ctgccggtgg tcagctcacg tcagcacagc tcggtctgcc cgccacgaaa aacgtcagcc
     6481 acctcatcag tcagcagcgc gtttttgtct gggcaaaaga aaaacccggc cttatgggtg
     6541 ccctcctgga acaaagtggt gattcggccc tgcttgccag agtggaaaac ggtcgcctcc
     6601 tggacacccg tgggcggcgt atttccatca cgctccctgc tgtgatcccc gatcaggtca
     6661 tcatctggat gaattaggcc ggactctgaa taactcacgg atttatctat gaacaaaaaa
     6721 cttcttgcta ttatgattgc agccactctg ctgcagggct gtaccagttt ccgacaggca
     6781 gagaacacca gaaaagacgc cgttgctgaa caacaacggg cccgggaaat gatcgctgag
     6841 ctgcgaaacc ggacaccggt tgtaaaggaa tccccacgtc agtggatcaa tccccgtccg
     6901 gtcacggata aaaaccccgg taatgcggtg cccggctgtc cgattatcat taacacccgg
     6961 cagagcatca cccttcagca ggtggcccag cggatctcgc aaaactgtca tattcctgta
     7021 aggatcaccc ctgatgtaca ggcgtatctg agcgtgacca cctccggcag tacgcagcaa
     7081 atccagggca cagtaccacc gccggatacc ggcggtatgg ttccactggc cgccataggc
     7141 agttctgaca cgacacttcc ccgcctgcag ggaaatacgg cgtcacttcc tgaactgaag
     7201 gcttcaggac tgacatcact gctgaatgcg gtcgcttccc gtctcgggat ttcctggcgt
     7261 tatgatggtt cacatatcac gttctattat cttgataccc ggaccttccc ggtgacctat
     7321 atggacagcc aggttgccta taatgccacc gtggtcagcg gtaccatgtc atcaaatggc
     7381 tcatcaggtg gaacagcatc ggatgccatt tccggtgatg ccagtaatac ccagaccacc
     7441 accgtggata tgaaatctgc gctgtatcag gatgtgaaaa atgctgtctc atcaatgctg
     7501 acgccgggta ttggtcgcat gttcatgtcg acgggcttta tcaccgtcac ggacacccca
     7561 caggtactgg aaacggtccg gacgttcatt gaaaaacgca atgaagaaat gaagcgccag
     7621 gtggtgctga atgtggaaat tctgagtatc cggaaaaccc gtaatgagca ggcaggtatc
     7681 gactggaacg cagtcttcag cgacagaacg ctgggactga gcctcggcag cacattcacc
     7741 agtgcagcca gcgataccgt cactggtggt gtttccattg tgaacgggaa actcaccggt
     7801 tcaaaagcct tcctgaaagc actctcttcc cagggagatg tcagtgtggt aacccggaat
     7861 tccgccgtga ccaaaaacct gaccccggta ccgatgcaga ttgccaatca gcagagctac
     7921 attgagagtg tgacgacgga taccaccgcc aatgtcggct catccacctc cctgaatgcg
     7981 gccaccatca ccaccggttt taatatgacc cttctgccgt tcattctccc agactcacag
     8041 acactgcagc tgctctattc catgagtctc tccgacaaac cggttattga gaattacgag
     8101 agtggtggaa gcaaagccca gctgccgaat gtggatctga aaaccatcaa ccagacagtg
     8161 gacctgaaat ccgggcagac agtcattatc agtggcttcc agcagtcagg gcgtcgcagt
     8221 ggaaaacagg gtgtcggcac accgggattc ttcggtcttg gcggtgggat caacagtgaa
     8281 aacgacgaca ccattctcgt ggtgctgatc acgcccaata tcatctgatt tgcagaagga
     8341 ggactatcgt gacagatatt tcaccgacag agcgacaggt gtttccgctg acggcaggac
     8401 ggaatgtatt tcttgccgga atggactgga aaacactgcc cgcaagccat aaaaaccccc
     8461 ggacgtttgc ccgcagtctg ggggcagtac gctatatttc ctgtgagtat ctcagtacgg
     8521 aagataccga tcgtcacatc atggttgcag ccgtcagcca gaatacactc cccaaaggca
     8581 gccgcaggta tttctctctc gccatgctta tcctgcctct gctcgagcca ggaggatacg
     8641 ctatcgttga actgtcacag gcaaacgata cagaacttta tggttttgtt tctgctgttg
     8701 acggcatcct ggtcagtgac ctggtgggaa cccgggagga aatcagggag gcccgggaaa
     8761 tattcctcac cattaacagc gcgcctgaac acggctggac atgttatgaa ccgccctcgt
     8821 ttaacgggcc tgacggcaga ggtcccctgc cactggaaac cctgacaggt gcagggaaat
     8881 atccggcaga agcacgcctt caccccgtat cccgtgggcc acagcttatt ccggtattac
     8941 tcatactcac ccttctgggt actgcatggt atggctggca gtactatgaa cgcctgaagg
     9001 cagaaaaagc agcacttgct gaagcggcac aaaaagccgc agaggaacgg atcacaccac
     9061 cgtggctgag tatgccggaa accggagatt ttatccgggg atgcagtgac gtatggtcac
     9121 gcctgccggt gtcggtcgcc ggctggcgat attcactcgc cgagtgtgct gccaccggga
     9181 acagcggcaa tctgcgtgca tcatatacaa acatcgccgg gacgacagtg accgcattca
     9241 gccagcgggt cagggaaatt ttcagcatcc ggccgtttat ggttatgccg gacgggaata
     9301 gcggtggttt tgccctgccg gtcaccttca gtatgcctga aactcccgtt gccgttgagg
     9361 cattaccgga aaacaccctc ctgcaggaac ggctgaccac actcgcccgg tcgatgcagc
     9421 tgaaaatgga ctggcaggaa gtcagcaaca gcttcaccga tgaagacgga aataccatcc
     9481 agcccccctg gaaggagtat gacctgcaga tactgacgac acttccggcg caccaggtgg
     9541 cagagcattt cagtgaaccc tccgtccgtt ttatctccgt aacaaggcaa ctggaagagg
     9601 gtcgtttccg ttatcaattc acaggtaaat attatgtcca gtaaaacaaa agctcttctg
     9661 ctttccgtcc tgatcctgcc gacaacggtc gcggccagca ccatcagcaa catgcaacag
     9721 aataaccggc aactccaggt ggaacagatt caggaagaaa ctgcactgta tgaagccatg
     9781 ctggcaagag aaaaggtgcg acatgaactc agaaccctgg agcagggaga accagctgag
     9841 cctgtgcatt cacaggaaag cagttcagcc gtcacgacag atacccctgc ccccattacc
     9901 ggtattcccc ggctgacaga gatatccgga gttcaccccc agataaccgc caggatacaa
     9961 ctgccgggcg gacaaactgc tgatgtaaaa gaagggcaac acattcccgg caccacattc
    10021 cgcgtggaaa agatcaccac tgacagtgtt gtccttgaac aaaatggtat cagaacaaca
    10081 ctacaaccct gactcagtgc cgccatttac agccggctgt cggaacagat tattcccccg
    10141 gcccttaatc acaaaaaaaa tcgtttccgg gtaccgctgc ccgtaaggag attttatgct
    10201 ggcacctgca gaccagccac ctgatgacat tctggattac gtggttgttg atacagataa
    10261 aacaggacaa attcatgtca gagtggtgga aggaaaaaaa caccttcgtg ccgtccagga
    10321 ttatctgacc cgcctgtgct cccgctatcc gggtaaagta tccccgtttg agttcaccac
    10381 acttgatgtg atcgcccgac tcaggcagga gacggtcatt tccggggtgg aaaacggaat
    10441 taaccccgta cagcaaaaaa tgctcgggta tatcagccat gcggccaccc tcagcgcctc
    10501 agatcttcac atcacgccgg ggcgtgacag tacggatttc acgtatctgg aggcccgtgt
    10561 ccacggagag ctggagctgc tcgatatcgt cagaaaagat gaggggctgg aattactggg
    10621 ggccacctat tccggaatga ctgacgtcat aaagggaaca cagtttgatc ccggtgtccc
    10681 ccaggatgcc cgtctggctg aaagattcct gaaactggcc ggactgtttg gtgcgcgcta
    10741 ctcccactat ccgtgtgtgg gcggactcta tgccgtactg cgtctgatca aggatgacag
    10801 tcagcatata ccgacattca gtatgctggg gtatcaccct gagcaggagc gcgctgtccg
    10861 aaggatgctg cagcgaccgg agggcatcgt gatcctctct ggccccaccg gttccggaaa
    10921 atccaccacg ctgaggacgg cttctgccgc ctatctggaa cagtatggct tcaacgatac
    10981 cggagggatc ctgctccccc ggcgacgtct gttcacaata gaatcaccac cagaggggcg
    11041 tatccccggt gcaattcaga cggccgttat ggataccgct cagggctggg tcgattctgt
    11101 taagtccgcc ctgcgccttg atcctgacgc tatcctgaac ggggagatac gtgaccatgc
    11161 ctccgccatc acggcgataa aagccgccat gaccggtcac ctgatgctga ccacccttca
    11221 tgctaacgac ccgataaata ttcttgagcg tctggaaatg gagggcgttc aggcaaggat
    11281 gattgcagat ccgcagttat ttatcggcct gctcagccag cgtctggttc aggtgatatg
    11341 tccccactgc cgcctcccct ggcatgaggt ggagagctcc cggacggatg aagaacgacg
    11401 cctggtggaa aacttctgcc agcccgatgc cgtgtatctt cgtaaccata acggctgccc
    11461 acactgctgg cgtggagtga acggccggac cgtcattgca gaagtgattt ccccggatgc
    11521 gaagtttttc cagatttacc gggaaaaagg ccggattgag gcaaaaacct actggcaccg
    11581 ggagctcggc ggtatgacac gaaatcagca cctgctgggc aaaatcaaca gtggtcaggt
    11641 cgatccgctg gctgcgcatt acatatcccc tgtagacgag gacagctata cgctgctgca
    11701 ctgatatgaa aattctggaa agactcaaca atacggaact ggaactgtcc ggccttaacc
    11761 gctggctggg caaaaagacg ttcaggcgga ctacgtttta cgagtccctt gcaggcatga
    11821 tccgggacgg cacaccggtt atgcgggcac tggagttcat ctgcgatgtg gaaacggatt
    11881 ttggtaaaaa gaaagggcag tccggcctgt attttctggc gacagactgt atcgccagca
    11941 tacgaagcag tggtcagctc tctccggccc tgaaggactg ggtacccaaa gatgaaattg
    12001 ccctgatccg taacggtgaa gagcgcggtg atatcgctga agccatgttt caggttgtca
    12061 aaacggcaaa aggacgacag gagatgatta gcagtctggt gagtgtctgc ctctatccgc
    12121 tcatcctgct gacgctctgt gtggtgaata tgtataacgt tcatacccgg gttattccga
    12181 tgatcagcgc ctttgcaccg gaagaacgct ggtcagtgca aatggtcatt ctggccaaat
    12241 cctccgagct ggttatcggt tatggtttgt ggctgctggc tggactgctt ctgctggcaa
    12301 ttctcatcag gatgtcattc agtctgtata aaggtccccg acgacggtat cttgatcgca
    12361 tcccgccgtg gtccctgtat cagacattcc atggcgtgaa ttaccttttt aatatttcat
    12421 ccatgctgca gataaatatt cctttatctc atgcccttac cagaatagaa aaacatgcag
    12481 aacataaccg atggttaaaa tcacgaataa gtgctatcag aaaaaatgtc ttatccggtc
    12541 agtcactggc actggccatg aaaaacagtg gttatgactt tccgtcaaaa tcctgtgtga
    12601 ataatcttct tttaaattca gaaagggagg acagtgttgc cagtatggcg ctttatgcag
    12661 accgctggct ggaagaagca aaaaagagtg tcagaaaaag cggcattatt attactgcta
    12721 tatcaggtct gctggtattt ctcttcatca tcaatatggt cagtgccatt tacggcatca
    12781 gtgacatgct gaagcagtaa acacagcatc ctttatattc ccctcgatcc aaattctgtt
    12841 ttaccaatct caggagctaa tatgagcgtc ataacctgtt cgtattcaga acataatatt
    12901 cgtcaccttc agaacgatga taaaggcatg accctgctgg aagtcctcgg tgttctggtc
    12961 gtcgctgcca ttgttattgg tgccgtgatg ggacttatgt ccgataccct gtcctcttca
    13021 gacaaccaga aggaactcaa aaacctgcag acaatcgcca ccaaaatgaa agcccagaaa
    13081 ttccaggggc agtatacagg tactgactac gtcaaaatcc tgaccgaaag tggtggcctg
    13141 cctgccgata tgatcgccgg tggtaataag gcgaaaaatg cctggggtgg tgcggtaacc
    13201 atcaaagtca gttcagataa atactcctac gttattgaat ccagcaatgt gccgaagaaa
    13261 aactgtattg atctggtcac cagcctgcgc agttcatcca tgtttacaaa aatcaacggc
    13321 aatgtgacga ataaagttga cccgtctaca gtatgtaacg cagataaaac tactatcaaa
    13381 ctggaaacca acagttaatc attcatcagc gcccgcccgg gcgctttatt taaggtcact
    13441 gtatgaacag gttatttatc ctgacagtga ttttttcact gttatgctca cttccttccg
    13501 cacaggcatc cccacaggac aaacaatatg atcgttgttt tattaccgcc ggaaaacgtt
    13561 attccattga tccaggatta ctcaaaatta ttgcccgcag ggaaagcagt ctgaatcccc
    13621 gggccattaa tcataacagg aatgacagag gaaaaattat cagtaccgat tacgggataa
    13681 tgcagattaa ctccacacat attcccgggc tgaaaaaaat gggcattatc cggagtgaga
    13741 aagatctgtt gaataatgtc tgcctgaata ttcagaccgg agcatggatc ctcgcccggc
    13801 attttcagcg atgcggcgta aactgggaat gcctcggcag ttataacgcc ggcttcagta
    13861 aaagtaacac tcaccgccgg atgaaatacg cccggcagat ttacagcgct tacatgcagg
    13921 gacgttagtc cgggagaact gccatgctcc agataatccc ggcactgctg gtcgccataa
    13981 ccgtggtaat acagtgtctg gccgttcaga tgagtcgcta tcgactctcc cggatgaatg
    14041 cccgcccccc tcaccatggg ctggcgctga tgagcgggat gcttgcagct gccgccatta
    14101 tcctgcgtct ttatcagaat gacagcgctg tcggggtgct gtttatgacg ataatgaccg
    14161 gatgtatgac actgatgacc ctgacggatg ctgcatcctg ccgtcttccc cggcccttca
    14221 ccatcatgta tctgataagc ggcagcgcat ttcgtctctg gcagggagac tggctgacag
    14281 gcctgtgcag tgcagggttc tggttcctgc tgttgcttct gttcaggcaa tacacctctt
    14341 accgcaggaa aacagaaacc ataggcctgg gcgatgtttt tctgattgcc ggtattgcca
    14401 tctggtcccc gccgggagac atcccctgga ttgtcgcaac ggcagcaggc ggagccttac
    14461 tttatctgtg gtgtaacaga caacgacgca ttaccagaga acttcccttt gggccgtttc
    14521 tctgcgcgtc acagtacgca tttacttttt atcccggtgg cctttggtaa taccggaggg
    14581 aacgactatg caaaaagaca acgataaagg ttttgcactt cttgaaattc tgggaggact
    14641 ggttgtcatc agcctgctca tgccgctgtt ctggtcttac attgaagact acctgaatga
    14701 aatgcgcaac cagagcgccg catttcatgc agatgcctac aatactgcag ccaggaccta
    14761 catcgccgat aacaatgccc gcctgcatag cggaacgctt cctgccacct tcacggcgga
    14821 tgagttaatc cggaaaggat atctgaaggg tcttaaccgc tcgccctttg ggcagagtta
    14881 taccaccggc atccgtcgta acacgagtac cggtcgtctg gaggccctga cctgctccac
    14941 cggaggggag aatatcaaag acgatgccct gcgcagtatc gccagcctgc tccccggtct
    15001 gggcggattt ataggaaaaa atggcacagc aaccggtgtt tttggtggat ggacagataa
    15061 acccggcgac tatggactca gctgcaatgg cgggcacatc gccatcgtaa tgatgggtga
    15121 tgacctgcag gaaagcgatc gcctgtatcg ctttcaggta ccgggacgac cggagctgaa
    15181 ccagatgaac acggccatca atatgggagg gaacaacctg aacaatgcag gaaacgtcaa
    15241 tggacaaagt gcaacactga aaggtgatgt tacaagcgaa aacggctggt taatcaccaa
    15301 aaatgataaa ggctggaaga acatcaccta tggtggcggt tttacgatga cagacagcca
    15361 gtggatacgc gcagtcggtg gtaaaggaat tataaccaca ggggaaatca aaggaggaaa
    15421 agtcagtggg gggacagtcc ggtctgacgg tcgcctttcc acaggtgagt atttacagct
    15481 ggataaaaca gccgtagcaa atacaaagtg ttcaccagat ggacttgtag gccgcgacag
    15541 caaaggggca atactttcgt gccaatccgg tgtgtgggtt ggctttgaat cctaccctgt
    15601 gggaagccct atcccgtggc catctgccac tcctccacaa ggctatctgt tgatgaacgg
    15661 ccagtcgttt tcctgttccc ggtatcctca gcttgcccgt gcttatccta gctgtaagtt
    15721 acccgatctg cgcggtgttt ttatccgtgg ctgggataac gggaagggac ttgatggtga
    15781 tcggaacaga cagctcctga gttatcaggc cgatcaatca ggcgtctatc acgaacgtgg
    15841 cggatggctg aaagggcatc atagtggtat gccatactgg gcacagggga gtaccacaga
    15901 aatgcgtccc aaaaacattg cattcaacta cattgttaaa gccagctagg gactcacatc
    15961 tgataggtat acacagagaa aacaccgtta ccacaggcgt aattttgtgt tggtctggag
    16021 atgatttgat atgacgctcc cactggcacg gcaaatgaaa taaatctccg ggaatatcag
    16081 ctggtcagca aactcgatgc caggcgtaaa cagaccagca aaatttcccc ttattttgtt
    16141 ccctaccctg caactgtcgg gtgcagaaac gggctgtttg tcgtgacact gcacgatttc
    16201 gaccttgaaa cccgtgcaga cccccgggga ctggcaatat ctcatgccag catgctcagt
    16261 cacctcctgt ccgaatcctt ttcattcgcc cttaaaacag agttgcaaac actcttgaaa
    16321 cacactcgat cataacgatc gaaataactc gcttccgttt aaaaaacact ctctttagac
    16381 tcaatttatg aacaaaacac gctgacccgc atggaggtga aatgaaaact gacagcacag
    16441 gtattgccgc ccgtatgatg ctctccctgg acagggagcg aatttgcgaa tgcctgctca
    16501 gtcatcgcca gctgcagtcc acacccctgc aggttcgtta tccgcaggga gtcagggatg
    16561 cccttggtat catgagcgaa cagctttctc tttccgtctc tgacctgacg cgtatcctgg
    16621 tcgaagacgc gttaagtgaa atgtttcttc ctgcagataa catcgtccgc cgtcttctca
    16681 gcaggatgga gcacatcatg caggcacatg atatcagcgc aacaacgatg gctgcattac
    16741 tggccccctg gaatatccgc cctgccgtct tccgggagcc ggacagactc actgactatc
    16801 tgaccgggga gattcttgca gcactggctg actggtttta tctcagtccg gaatggctga
    16861 acggcagggt gcattatcca ctgtatcgtc cgggagactg gccagccaca caggagatat
    16921 tctgcaggat tatttcagcc cgcgagaata tggacattat tctctggcat ggttttccgt
    16981 ttgccggaac gcattccgga gaatattgcg gcgttctgct caggcagaaa aaagaaatta
    17041 ataacaccat tatttatccg gtactttcgt tatatccggc cagaatggat atagaaaaag
    17101 aaggctggtt tcagatggcc cgaaaaatat ctccggatat ccctgttcgc gccgtaacac
    17161 tcactccggc acaggcagaa tatctgataa caggaaaaat attacccact gcgttattca
    17221 gggttccgtt atccccctgg taataatact gagcaataaa ccggaataat acaccaactg
    17281 tttttaatgg cacagccgtg ccctctgttg ttatacccaa aatacctcaa tacaggaaac
    17341 tgaaatgatg atgaaaaagc atattacccg tactctcatc gcgtctgccg ttttattttc
    17401 ctttaattcg actgcagcaa cgtcatattt tgaagcccgc aatgatgcga tgggaggaac
    17461 gggggttgcc tcctctcact atggtgtggc cccactggca aacccggcac tgctgacaaa
    17521 atccggtgca aaagatgact tcagtcttct gcttccgtcc gtgggtgcgc agctgtccga
    17581 tccgggtaat atcgccgaca acgccgacaa aatcagcgat gactggaaag ccttcgaccg
    17641 tgccgtcgac agccactccg gggtaccgca gacagcggcc cgcctgaaag agagactgca
    17701 ggacttccgg aacacccatg ccagcgcaca ggccggggta tctgctgtgg cggcccttcc
    17761 cggtgatacc ctcgctgctg cactgatgct gaaaacgctc gggactgtca gcgtcgacgg
    17821 taaagtcagc gatgccgacc tgaactatct ggaaagcatt gcagacagtg gcagccagga
    17881 tgtggataaa aacagactga cctcacaggc ttttgcccgt gccgcactga tcaccgatgt
    17941 gggcgttgcg ctggccactg aactggaaac ggccggacaa aaatggtcac tgggcttcac
    18001 accaaaattc cagcgtgttg acctgtttaa ctacaatacg ctgatcaaaa actatgacag
    18061 cagcgcattc aaaggtgacc gttaccacaa cacccaaaac ggtattaacg ctgatatcgg
    18121 tgccagcatg gacctggatg acaactggac gctgggtctg gtcgcacaga acctgatccc
    18181 acgcagcatt gagaccaaag aggtacatgg tgtcactgaa accttcagga tccgtccgca
    18241 ggtaacaacc ggcgtatcat ggcacaacga catgttcacc acggcactgg atgtggatct
    18301 gaccccggcc agcggcttca catcagacag caaacgtcag tttgccgccg taggtgctga
    18361 gtttaatgcc tggaaatggg cacagttacg taccggttac cgccagaatc tggccagcaa
    18421 caacggcagt gcattcactg ccgggattgg gctctcaccg tttgatgtga ttcacattga
    18481 tgtcgccggc ctcgtgggca ccgacaacaa ctacggtgcc attgctcagt tacagttcac
    18541 cttctgatta tcacgccccg ggacgcccgg ggcttctgac ctgaatctgc tcgtttccgc
    18601 tcttctggcc actcaccgcc agtcctgttc attattttcc ggataaaacc atgcaaaaaa
    18661 aacgtatcac ccttgcaggg ggagttgcca tgctctgtct gagcggctgt catcttcccc
    18721 cggataatac cgcaccggcc aggaagtacg gtgcagcttc tgaagtcgct gtcacacgac
    18781 atgcacagta tcagttgtgg gagcagggca cgtattctgc agccctgcct tcccgccagc
    18841 ccggaggcag cctgcaggct cacagcgggc gggtttcctt tgactgggaa ggcgatgctc
    18901 tggagctcct gagcgagctg gcccgtctgc gcggacaaca gttcagttac agcggcgtgc
    18961 gtcttcccct gcccgtctca ctccatgtac gcgatatgac cttcgctgat gcccttcgtc
    19021 tcacagaggc ccagactgcc tggcgggcag tcctctcaca gtcagccggg cgtttacatc
    19081 tttcctatat gccaacggag aaaaaatgaa aaaacaggtc tgtctcctgc tgtccgggct
    19141 cctgctggca ggctgtgccg gacagcacca tcgcccccct gccggtatgg caggcaattt
    19201 accaccgccg gatctccggg catggctgac gcccactgac aaccaggatg acggcatttc
    19261 agacacccgt cgccagatgc tcacggacgc cggacgaacg ctgggattcc gtggcggaaa
    19321 agcacagcgc tcatgggaac tgattcaggc actgaatgcc cgggaaagta cactgaatgc
    19381 cctgtatgac ttccgtcccc ttatcagcag ggaaggctgg ctaccccctg ttattgatga
    19441 agcgcaggat gttgctcaca ttcagcctga ccagataaga accgcgtccc gtgtctggac
    19501 catcctgcgc cccgaacgtt ttgtcagtaa cccgccgggc tggcgtgact ggcttctgcg
    19561 ggggctttcc actaccgcca ccccgggaac tgagggccgt gttgtaccgg aagaccgcgc
    19621 acagcgcaga ctctgggaaa acgcactccg ccagggctgg caggaaggcc gtgacaacgc
    19681 tgacctcacc ctggaagcca accagaaacg cctgacccgg gattaccggg gaatgatgct
    19741 ttatgccctg ctctggcgtc agggcatgat cacacgcccg gatgtgactg aacagcggca
    19801 gaccgtcacc ggtaacggca ggaaactcat caccggagac cacgtccgcc gtctgaagac
    19861 acacgcggaa ttcaccctgc agaaatcacg ctggcgtccg gtcgtcagca cggaaggagc
    19921 ccccgatgaa attacccgat gagttcggca cattcccttt cgcccgtttc acggccgaag
    19981 agtttcgcta tttttttgcc tggtgtgccc gacaccgcgt cagtgatatc gatctgacag
    20041 gcggctcacc ggtatctgtc agtcgctttg gacggcgggt acgttcgtcc gcacatcctc
    20101 tgccgtcctc catcatgatg gcgctgagtg atgaattgtt tggacgggaa gtccttcccc
    20161 gggtgctgtc cggtcatccc gtggaccgta ccattcaggt cagcggtgat gcctccggtc
    20221 gctacggtct gcaacggggg cagcgtgttc gcctgcgctg tcatctgatt cagggaacat
    20281 cagcaacaga agaaaaagta atctccgtca ccatgcgggt gatcccgtcc gatattccgg
    20341 acatcctcac catgaatatt gaaccggacc tgctggatgc catgctccgg aaaaccggtc
    20401 tgggtttcat ctgcggagag accggctccg gtaaatccac actggcagcg gcactgtacc
    20461 gctacattca gacgcatttc cccgaccgca aaacggtgac ctatgaagat ccggtggaat
    20521 acattctggg gggagagaat gaccttctgc caccccatca ggccgaaatc gggcgtgatg
    20581 tggaaagttt tgcggcagga cttcgctctg ccgttcgccg taacccggac atcatcggta
    20641 tcggtgaaat cagggattat gaaacggcag atgcagccgt ccaggcaggt aacaccggtc
    20701 acttctgcat cggcaccatg cacaccaaat cacccggtga aacctttgcc cggctgctgg
    20761 ggttatttcc accggaaatc agggattcaa tggcagccgc caccctttcc ctggtgcagt
    20821 tcattctggt gcaggtcctg gtcagaacaa atgacggtgg cagacaggcc gtccgggaat
    20881 acatcgtcat aactgacgaa ctgcgtgata cgctctcccg gcagtcccat gcgacatggg
    20941 ggcattacat tgatgaaatc attcgcaagg aaaaacggcg gatacgggat caggttctga
    21001 ccatgtatca gacgggcagc attgaggcat cagaagctgc gctcttcatc ccggccggag
    21061 aattccccaa atgaaacgcg aaggcttctg gcgggatgtc actccgccac tgaatattta
    21121 tggtgtcccg ttgccgttta ccatgattta cctgctgctg tttccgtatc cgtcaaaaac
    21181 aacattctgg gtgtgcagcg gcattttgct gttttttgtg ctcctggggt actcaggatg
    21241 gactgtccgg acgctctgcc ggcgtctgta cagcctggtg cagggtaaac aggcgtcagg
    21301 ccgtccctgg tggtaccgac atcgcacaga attaccccgt gaatggcgcg gaacggaata
    21361 accctctttt gttcatcatc ccggcgtatc acaacgccct catctttaat ccccggagaa
    21421 cgacatgaag tccagatatc gaacctggct tgctgtcccg cctgaggaga cagaagccgt
    21481 aaaaaatgca gttcccccgt taaatggccg caaagctgtc gcatgggatc cagaaaaaaa
    21541 gctctggtat gcccgggcgg gtaccgaact ttcactgctg gagcgctggt taccccggcc
    21601 acaggaactg tcgatggatg ccggcgatcc ggtcactgaa tttgctcagg tcctggaaaa
    21661 cgccgggctg gttattcagg gattgccaca gatggacgga gccatacaca gagtggccac
    21721 ccgggatgac aaaaaagggg ccaaaagtgg cgcatacagg gcctatcttg atggtcgtcc
    21781 cgccggctgg taccgcgact accggagcgc cgatgacagt cccacaaact gggtattttc
    21841 cggcggagaa caacacgatc ctctggccag actgcatctg cgggcattcg ctcagcaaca
    21901 gcgcgatgat aacgctcgta aactgcagca acagtataac aaacaggccg gctatgcccg
    21961 ctcctatatt aacaggttgc cacaggcaac agcacatgaa tacctgacgc gcaagggcat
    22021 ccgggccgcg cctggcgtgc ggctgaacaa taaaaatgag ctggttattc ccttcagcaa
    22081 cggccgggga gaaatacgtt cgtaccagcg tattccggtc accggcggga aggatgcccg
    22141 cattctgaaa gactccgaga aaaccggcaa ctggttcacc ttcggaaccc cggaaaatgg
    22201 ccgccccctg ttatttgcgg aaggttacgc tactgccgcc tccctgcacg aagcaaccgg
    22261 ccttccggtt ctgatgacag ttgatgccag caatatgatt gccgtggcag aaaatgcccg
    22321 ccaaatctgg actgacagcc cctttgtctt ctgtgccgac aacgatcatc aacgggaaat
    22381 taacaaaggg gtttttagtg caacaaaggc cgcagaagtc acgaatggcg aagtcattat
    22441 tccggccttt acggaggcgg aaaaagcaca gggactgacc gatttcaatg acctggacgc
    22501 cagccgggga cgggacaact ttcagaatgc catgaatgca cagttaaaac atatcggtat
    22561 ccccacaccc aacagtgaca ctgccgacca tcgtgaggct gtcgtgatcg acaacctgat
    22621 attcacaccg gtaaaagatg agaagccaca aatgtcccct gaaaacagac agagcacagc
    22681 cccggaaacg gagctggcga cacaggatac gccttatgac acccactccg acgatacaga
    22741 aaaccaggca tatgaagatg actatgccgc ctatgcatcc ctgcaatcag taactgcgga
    22801 gacgttatcc ccggataaac cactggagaa tattgcgtct gccgctgata ccggcacaga
    22861 gaatacctct gtgcctaccg ctggcgacca ggaagacctc aatacagacg ccacaggtac
    22921 agacaccgct cacaacagcg acacagcaga aacaggaacg cttcctgaac ctgccgggat
    22981 gcagtccgtg cagcaggcaa ccactgaacc gaccgcagaa gataaaccac acgagaacgt
    23041 tgtttccgct gccgatacca gagaagaaaa tgcctctgtg gctaccgctg ataccggtga
    23101 agaaaatgcc tctgccacca ccgctgacaa ccagagcacg caggcagaag aacatccccg
    23161 gagtgaagac acaccggaaa cctccctctc cgacgcggcc gggacacagc acaccaccac
    23221 aacgcagtct gaagaacaag tgcaggtttc catggccagc gctgacgact ggagcgcatt
    23281 tgcggaagat ctcaacgctg acactccggg ggcccccact gttcacaaca ccgacaacgc
    23341 ggagacaaac acagtttctg aaccggccgg gatgcggcca gttcagcagg tgaccactga
    23401 actgacggca gaagataaag cacaggaaaa gagcgttgcg tctgccgctg agaccggcgc
    23461 agagcactcc tctgacatca ccgatgacaa caggaacgca cccgctgagg agctcaatgc
    23521 cgatgccacg gttgctgttc acaacaccgg cacagcagaa acagcctcac cttctgaacc
    23581 tgcagaaact cagccagtcc ggcaggccac cgctgaaccg acggctgaag ataaactgca
    23641 ggaagagcgt cttgtgtccg ccactgagac cagcacagcg aatgaagcag cccgccctga
    23701 aacgccatcg cttttcagcc gtctgcgccg ttttctgacc ggtgtgaaat cacgcacctc
    23761 agaaccttcc gttattcagg agaaaaagcc cacggaggct gaaacagcaa cagaggaggt
    23821 cgcaccacca tcaccaccgg aaaccagccc ggaagacaca tcgtcagcgg aaccggacac
    23881 gattatcttt gcaccccgcc gtcccgacag tccgcagcgg cacaacctgg acgagatcct
    23941 taaaaatctt acacacaaag agtttcccga caggactgcg ctctacagcg ttgacggtga
    24001 acccgccttc ctggacagac tctactgtct cgaaatggtg gatggcgcca gcgcggatga
    24061 caaaaaggtc ctggctgccc ttgctgtggc caccaacttc tatggcggcg ttatcgagct
    24121 gaccggcagt gatgccttta agcaaaaagc catgcagctg attgtcgaat acgacatcaa
    24181 ggttcgcatg aaactcccgg cccagcgggc agcactggaa aaactccgca aggaaaaaga
    24241 gatggcgcat gatgccattg tcacccacag acccacgccg gaactcaacc gccagtcacc
    24301 ggaagagaca acaatggccc aaccggctcc ccctgtgtca ccgaaggcag aggctgaaca
    24361 aacagcaggc agtacggctg aaacaccgca tccggaagcg ccggtcacgg caaaagctga
    24421 agccccccgg gccgacagac cggaacaccc cgccgccccg tcgtctgcgc cagaaaaagc
    24481 ccctgtcagt gcacagccgc agcaacccga cacaccggtg gaagagccgc ccggcaagct
    24541 cagccccggt gaaagtgtga ctgccgtcct gacaaactac ggagaaaaag agtatgcgcc
    24601 cggaaaaggg cgctgctttt ttgtggagct caccaaccgt agtggcaaac gggaatactg
    24661 gggaaacggc ctggaagaac tggtcaaaaa ccatcagaag ggtgaccccg tcacactgac
    24721 actgcaggcg agagaaacat tccggctgtc cggtgaaacg cgggatcgta tccgtaatat
    24781 ctgggccata accccggtca gtaacggcat taccgttaat aatgaccaac ctcatgcggg
    24841 tcagcatacc ctggagtttc cgatggcaac attcagccag gtgatgttac agatgcaaca
    24901 acactggcca cagataatgc aggaagttcg ctcccccaaa aatcctccca ataatctgta
    24961 cattggtgaa gacaggcaac ctgcatcagc acctcaggat caatcaatgc gactccccct
    25021 cgctggtaat gcccccaatg aactgatacc agccatcgcc tctgccgata aggacaatcg
    25081 tcagttgaat cttctgctgg tccacagcgc agatgatcat ctgcagggcg tggtccgcct
    25141 gaacggcacc ctttacccgg cactggccac cccgtcagct gacaaccgtc agctggttat
    25201 caatgccctg acggataacg gtctgcagtt tgccggttac ggtgaagctg ttaaccatga
    25261 tgagaataat catcagcgtc cgtccccaca aataatgcag tttcacctga agcaacagga
    25321 ctccccgttg tttgccgcca tccacaaacc ggaagagcaa ccggacaagc ttttccgctc
    25381 tctgggtttt gagcagacct ggaaagagtg gtctgacagc caaaaggcgg aggacaggca
    25441 ggaaaaaact ctgcaacagg cacaatccca cagccccggc cgatgagccg gtaaggacga
    25501 ccatgaacat gaaaaaaacg ctgctggtca tactgaccgg catcaccctg gcatcaccgg
    25561 tggcaggtgc cgccacctgc cgggcggggg ccgctgccga agccggcagc agagccgcct
    25621 gggagcgggc gcgcacagcc agccaggcat ggtcacagcg ggagaatgac gtcgcctcct
    25681 ccctgcagtc ctgcctgtcc cgcatcaagg acatcagtat cactcttccg cagttcccca
    25741 gcctggacaa gctcctccgc gacctggaaa accaggtctg tgatgccgtc gtggatcggg
    25801 tgaatgacca tcttccgggg aatatcgacc cctggaaaga ctacaacctc tgaacaggag
    25861 caccatgagc gaagcacaac ccacctcccc tgtctcaccg gaagcggata acagcggaga
    25921 tatttataac cccgtgctca gaaccatcgc acagtcagaa caacgggccg cgctggtacc
    25981 cggtctggtc agggcgttgc tgtacagcgg cgcagcactc tgcatcagcc tggtggctaa
    26041 tggcatacag tactggcatg ccaccggcgt gacgcgcgaa tactttgcca ctgacaatgg
    26101 ccgactggtc cgtctggcac caaccagcca gccggcctgg agtcagagcg acgtcatgaa
    26161 ctttggcagc atgacgctga gcgaagcctt caatctcgat tttgtccact accgaaaaca
    26221 aatcacgaca caggcccccc gcttttccga agccggcttt gccggctatg tgaatgccct
    26281 tcaggcctcg aacattctcg acactattaa aaaggagaaa tttaacctca caaccactat
    26341 cggggcaggt accctggtca accagggaca gatggagaac ggcgtctggt tctggaaatt
    26401 ccagtatccg gtcaggatgc gcctggtcgg tcagaccagc acccgccccg aacaggcctt
    26461 cacctttgaa atcactgtcc ggcgcgttga cccccgcatc aaacccggcg gcatggaaat
    26521 cagccagatg gtttcccgta acgccgcagc aggaatctga gactcatatc atgcacatac
    26581 atcatcttat tggcgcactg cttattgtgt gcggcactgt cacagctgca gataacccgg
    26641 gctggaaaaa tgcacgaagc ccgcagaccg gcggaaagac cgcacaggca accgggaaca
    26701 ccccggcagg cggtcccccg ccagccgcac aactcccggg aaacctgccg cctcccggcg
    26761 ctgctcttgt acaggaaggc gcatcgctgg attctcccct gaatgccagt gaaattcgtg
    26821 aactccgtgg gataatggcc gataacgaac gggccatcag tgccccgctc accagtgtgg
    26881 tgccccgtat cagttccctc acggtgaatc tgtctccggg ggccagcctg ccccttgtcc
    26941 gtaccgctct gcataacgtc agcgtggtga ccttcacgga cgtcaacggc agtccgtggc
    27001 cacagtcgga tccgccctat aacgctgcgc ccggtcactt tgacgtgcag ttcaacggca
    27061 atatggtgtc cgtcaccccc ctgcgcgcct gggctgccgg gaatgtgacg gtctatctca
    27121 aaggattgtc tgtaccggtc atcctgaata tcaccagtgg tgaaacggac accactaccc
    27181 tgagtcagga aatcgacagt cgtctggatc tgcgcattcc ccggcaggga cctgccagcc
    27241 cggtggtgtc cataccgtca gaaaaaattg ccctgcatga cgccactctg caggcctttc
    27301 tggacggtgt gccaccggat gatcccgcca tccgccgcct gaaattctcc ggtaacgtcc
    27361 cggacaccac catctggcaa cacggcgatg atcttctcgt gcgctcacgg gccatgctgc
    27421 gggatgaatt tgaacagacg ctggcatcgg ctgatggtac gcggctgtgg aaactgccgg
    27481 tgaccccctt actcaccttc tccgtgaatg gtcagtccgt tcacgtcacc ccggaactgg
    27541 agtaaatcat gagcgcagaa caagaacagg acacagccgc aaaatccggc aaaaagctgt
    27601 cagccctgct ggttatgggg ggcgtgatta tcatcggggg cggatacctg ctgctcacct
    27661 ggctgtcccc cgaaaacgct gcatcgctct cgtcagtcaa tatcaacagc acaacgtccg
    27721 gcggcggacg gaacgtgatg gagacacagc actaccggga tttactgcgg gctgacaatg
    27781 aacgtggtgc tgcagaagcg ttacgtaata acgagacgtt tatcgccagt ctgccccggg
    27841 gactggatac cgataccgga aacgatcaga aaagggataa tccccccgat gatcagtcag
    27901 gcacttccgg gcaaaacgca tccccccctc aggaagatca gactgcccgg gaaaaacggc
    27961 gtgaggcatt acagaaactg cttgcccgta tcaatacaca gcatcaggat gcccggccac
    28021 cggtagtggc agcagccatg tggagcacca ccggcactcc cgggacagcc ccacatgcgt
    28081 caccgcccgg taccgttaca tcacaacatc cccgtctgca ggagacgagt ctggcaaccc
    28141 cggcagcacg tccggggatc cagctgatac cggcactgac ccgcgtaccg gcctacattg
    28201 ataccgaagt ggactctgac aatgcggtct cccgtgtggt ggcctccgta cctgccggcc
    28261 catgggccgg ggccgtcctc tacagccagg acgccagact cgcaggtcag ggaatggata
    28321 tccactttga ccggatgatg tggaggggca tgtttctgaa ggtgaatgca tatgccctga
    28381 atgaacgaac aggaatgtca accgtggcca gcgacgtgaa ccaccgctgg tttaagcaca
    28441 tcgttctgcc gtccgtactt ggcggggtgg gcaatgtcgg aagtctttac aaagacgcca
    28501 acacacagat ccttcaggga aactacggca gtattaccgg cagggtggga atgcctgatg
    28561 gagaggcgct ggccggcgtc attgcaggtg gcatggccga acggggcagc cagctgctga
    28621 cccgccaggc agagagcgaa ccttaccgtc aggtgacagt atcacggggc gaggtcgtct
    28681 ccattctgtt tgttgagccg gtcatgagca acgatatcag tacgccgtcc tccggcactg
    28741 ccccttctga gtccccctcc tccggacgtc cgctcaccca ggcggaggca gagcaccagg
    28801 cctctgacag gatccgggcg gcggtcctcc gtaaacagaa tgaaatgcag caacgctacg
    28861 gcatacaaca ggaaagttat ccatgaaaaa cgaacatgaa gatcccggca gctttggcga
    28921 aacgacggca ccggcatccg gcaaatcctt cctgaaacgc cagtatatgg gatacagcct
    28981 gccggtctgg gccggctgtg ccctcatcat cctggtcgcc atctggtaca agtttctgcg
    29041 gacaccctca gagccccctg cagatgcagg ctttgctgtc acaccgcccg ctgctgagag
    29101 ctgggcagag cccatgacca tgacggagtc cgggagcgga aacaacatgc ccgcaccgcc
    29161 ggttacccca caggcagaca tgcagcaact tgcccgtgat ctgaaagcag agctggacag
    29221 acgggatgag gaaatccggc gcaccctgac catgctgaag gacagtatca ccaccctcag
    29281 taacgccatt cagaaggatg aggcctgggc acaggaaacc cgtcagttgc ttgaggagac
    29341 ccgtaagcag gtgcataccc tgcagatgca gggagcactg ccagccccga ccgggaataa
    29401 cagacagcct gcgaaaaccc tgccttcacc tctgcagggc atgaaaatca tgtcggtgga
    29461 gaaaggcatg gcctggatcc gctggcaggg cagtgcctgg gcagtacggg aaggcgacag
    29521 tctgggaaac atcaccatca cccggattga cccgtccagc cgggcagtca tcacctctgc
    29581 cggtacatta cgatgatgag cattgatgcc ctgacagcca tagagaactt tgccagcggt
    29641 attttcagtg caggcatgga gtttctcttt acctggggag agctgctggg gattatcggc
    29701 cttatcggtc atctgatgcg cgcccgggca gaaggacgtc acagtatggg accgggaaaa
    29761 tttattgccg gtatcttcat ctgcggcatg ctggtcagtc tgccctcaat gatcaatgcc
    29821 ggtggtaccc agatgggctt cagggcagat tctttcgccc ccatcgcata cgtgcagccg
    29881 ggctcctttg gtgccgctgc cggtgccgtc aatgccattc tctcactggc aaaactggcc
    29941 ggcgtcggtt ttgtcatgaa cggcatatcc atctggcgca aatcactgct ggacgggcac
    30001 accgccctga gcgccagtga atccatcagt aaagggaacg tcaagtttgt ggccggcgtt
    30061 ctgctggttt tcaacgaccg ggttatcgat gccactctgg caagcctcgg tatcgccttc
    30121 tgacccctgt attttttcag taaatcaatc tgttaataaa ggagacacta tgtcacgact
    30181 tcgtgccatg gcggcgcgtc tgcgcgctgc atctgtttat gttcagttat gcctgatggc
    30241 ctgccgtgac cggctggtac gcctgttact gctgcttccc gtgatgctgc tgccccacac
    30301 cgcaatggca aataccctct tcgatatgat taaaaccgtt ggcgatggtg ccagaggctc
    30361 cagcagttcg tttgtggcta tcggtcaggt tattggcctg gtgctcttta tcggcggact
    30421 gcttggcctc aaacaaatcg gtaaaaaacc cggtgcaggg ctggcctgcc tgctgtcaat
    30481 tctggtcggc gtctgccttg ccgtcgtacc ggaactgatg ggcagcggac agaaaacgct
    30541 cggtctttct ccggtttcaa tcagttaatc cgcaaaggca ccgggaaacc ggtgccgtct
    30601 tcacacaatg aggaggacca gaccatgtcc tgtgagctac cccctttcac cctgctcaga
    30661 acagagcacg gcaacgtgat tgcccgcgac aaccgggata atcaggagtt tgtgtttgca
    30721 gagtgttact ccaccgctat agcagcactg attctggctg ccgtcaccct gtatacagag
    30781 agaaaagcgt atgaaaaaga ccccgccact catcgccccg gtccgggaac gatggctttt
    30841 cccgctcccg caaccggaca cggtattcag cggtgaagcc ctgttaccac cgccagtgct
    30901 ctcaacagag ggacgctgcc cctgctgtcg caggaccgtc acccaccgct tcattctgga
    30961 agattcatgg ccattacagc agatggctga cacctgccgg gatacgatcg tcctgctgga
    31021 aaaaaacctc acccgggtga tgcgcctgaa aaaacgtccg gtaccggaaa acgctgacga
    31081 aaaaaagaaa cacacccgaa ccctgcagga tgcggagcgg tccctggcac aggcccgcct
    31141 gtcggccaga cgactggccc tgcgtcatgt ggaaaagtcg caaatcgtca caacggatgc
    31201 actcagcgaa aatgaaagtg acctgctgca gccggaaggc ccgccctttc atctctgtgc
    31261 cttctgccac gcctggcact gtctgaacgg ctacgctgcc gcacagggag tgatggtctg
    31321 gctccccgat ttgcatccgg cctcggtggt tgccctcaat gcgcgggcac tgcaggagat
    31381 tttcagcgat aaccgtcagc gggtcaggca gggacgggca gtcctgaatg ccctggtaca
    31441 gaaccgtctg gccgttgagg agaaattccg cacctggcgt ccggcggatt ttgctgatgc
    31501 cctgcgccgc tggccgccgg cacagcgtaa aacgttgagg gagaaaatgg acggggtggc
    31561 gctcattctg ctgccggatt cgtttcctga caaaaagtac gtcatgtgac acacaccgct
    31621 gccggtgacg ttttgccgga tatcagttct ccgcagatgc attccggcac tttattatcc
    31681 ggtcgctgac tgacgatatt ccacctgacg gagatgaaaa agagattgcc cggttcattc
    31741 gccgccagca gtttatttat ccgggtgtaa tcacacagaa atacgcccct gcccctcaac
    31801 atattattta tcttctctgc tgttttttca tgccacatcc cctgctgtgg catcaaaaaa
    31861 tataagaccg tgatgattca tccttaccgg agtgccccat gccatgaata tcaaccgcat
    31921 tatttttgcc attgaagact gtatcagtac cctgtcacgg tattccgttt ccgcattctt
    31981 ccccgattac tgtgatctgc acaccgtcat cggtctggac cagcaggacc gcgaacgccg
    32041 cccctggctg aaagccccct acattgccac cacaaaacag gggtattacc tttccgtctt
    32101 tgaaatcagc ggtgccctga aagaaatgga tgaaaacccg gaccagacgg cccccgggac
    32161 actggagtca ctgattgcag cactggctga gcgaatgaac acggcctgga aaaattccgg
    32221 ccacaaaatc agttttgttt tcgaacgcga tccggagcgt ggaagggatg aaatcgaagc
    32281 catgctctta ccccagcgaa aatccatcgc acgcacgggg atcctgcttc aggacgtcct
    32341 ggatgaaaaa gtcaccacac tcaccccctg gcttgtccgg gagcgctgct ggctgaccgt
    32401 ctggagctct gaagaactgc tgagccggac cgatcgcaag gaccatcaga cccgtgtcag
    32461 gaaactcgct gaacacgctc cgccagcccg ttttgcgcag gatccctggc gctggacact
    32521 ttccgccctc aaaatacggc atgacgcctt actggacacg ctggagcagg ccctgacaca
    32581 tgattctgat ggcctgctga ttcgcctgat ggatatccat gaagtgggac gggaaatccg
    32641 ccgccagctg gagcgcaaca gtacaccggc agtatggcag cctcatctgc ctgaagatgc
    32701 ccgtcctgcc ggctggcgac agggcgggga tacctctgta ttccacgcac cgtcgctgaa
    32761 tctgcagtta ttcaccacac agcctgaaac acacggcagc ctgattcagg ccggtgagct
    32821 ctggcacggt atggtcgcca ttaccctgcc ccctcagaat ctgcggacat tcaaccagct
    32881 ggtcagggat gtgccccgtg caatcccctg gcgtatccgg atggatctga tgcccgacgg
    32941 catgaaggcg ctgggggtca agaaaaccct gctgacctac agcagcttta ttcctcctct
    33001 ccggcccatg tatgactcgg taatgatgct gaatgacatc aacaagcatg atccggtctg
    33061 tatcatgacc attgttgcca ccacatgggg aaacagccgg gaagtctgta cccgtaacca
    33121 ggctctcctg aaatctgcgc tggaaggctg gggggtatgc ggcaccacca ccaccttcgg
    33181 tgatccgaga cgtgcctggg tgaacacggt tctggcagcc tcccatagct ccggcccgat
    33241 ccccctttac ccgccccttt cacacgccct ttccctgttt cccctgaaca gggcaggctc
    33301 tgtctggcgg ggacagggaa atctgatgat gcacaccgaa gacggctctg catgggaggt
    33361 ggcgctggcg tcatcacaac agaacaagca cacggagctg acacccggcg cacccggtct
    33421 gggcaaatcg gtactcatca acgcactgtc ggagatccag atagcctcag cccagaagaa
    33481 tctccctttc attgcctata tcgacaaggg attcagtgca cagggactgg tacagcttat
    33541 ccgtgacagc ctgcctgagc agagaaagga cgaagccgtg gggatcattc tgagtaatga
    33601 cccggaccac acccgcaacc tgtttgatgt gatgtacggg gccagaaaac ccgtcacacc
    33661 ggaaaagaac ttcatggtct ccgtcctctg tgcattgtgt gtggataccg gcaccggtca
    33721 gccctgtaat cccggcgaca cccggcaaat catcagcagc ctggtggacc tggccttccg
    33781 ggaatatggc gagaataacc ccagacttta ccgcgcaggt acggaacccc tggtggatct
    33841 cgccctggaa gagagcggca tcgctgaaca acatgatgcc ggatggtgga atgccgccac
    33901 ctggtttgaa atcagggaca tgctgcatat tgcagggaat atccccgccg cccagcgggc
    33961 acactaccag gccatgcccc tgctggctga aatgtccgcc ctgctgggac agccgtccat
    34021 ccgggatgtg ttcggcaccg ttcagcgtga taactcggaa gagcggttgc tggactacat
    34081 ccgccgtgcc ctcgatcagg gacacagtga ctacccgatg atgtccggct gtacccggtt
    34141 tatgctcagc cccgacaccc gcgtggtcgc cgttgatctg aacaatgtgg ccggggacaa
    34201 gacacctgcc ggacgtctgc gcaccggtat catgtatctg ctggccgggc agatcgccgg
    34261 cggggatttt gtcctgccgc agtatcagga ggaaatacgg aaaaatctcg acccacgcta
    34321 ccatgaggtg attttccgcc gcattgaaca gcttgatcag gaggtcaaga ccaaagtcta
    34381 tgacgaactg cacaatgcaa aagggatcaa cttcatctgg gaagccctgg acacccagga
    34441 gctggagcag cgtaagttcg ggatacgcac ggtgctgagc acccagctgc tgcagcatta
    34501 tcctccggcc gtgctcaaat ccgccaacac cctgtggctg ctgcgttacc gccctgacga
    34561 aattccgttc ctgcgggata attttggtgt ccctgaagtc accctgcggc gcttcctgaa
    34621 aatgccggaa ggggccgcac cggacggcag cggtgtcccg gtactggctg tgttccgtgt
    34681 aaaaaacggc acgctggccc ggatcctgaa attcaccctc ggtccactgg agctgtgggc
    34741 actgaactcc tctcctaaag acagcgccct gcgacgtgcg ctgacgcagg aggtggggtc
    34801 actgcgggca cgccagatcc tggccgaaca ctttccccgg ggttcagcca cctcactgat
    34861 agaacaccgc gcccgcacac atgacagtga aaacgtcatc catgagctgg cagcagaact
    34921 catccgtaag cagggatata acctctgatg tacagcattc atatcacccc ccgctgccgg
    34981 cgtcctgccc ggggacaggt cttcctgaaa acgctgcgcg tggacggtga cagtttcagc
    35041 ttcaccctgc cgtccgcctg cttcacactg cgaccgtccc ccaatccccg ggtcattttt
    35101 gccagtgaca gacgggctga cggggagatg agtggtattc gcctggccct gcccggagag
    35161 acaggcacgc tgataacaac ggccacctgg caggttaacg tgccggagcg accgcctttt
    35221 gatatacagc atgtcattca ctggcttctg cccgcaccac tcactgcgct gatgtctgac
    35281 aacacctggc tgtggccgga tgtccccggc acaccggcac tcacagccgg agactggccg
    35341 gtcatggaaa gcgacacccc gacactgaaa cgcgtgaagc gtcgtacctt cacgctcatc
    35401 gataaccgta accgtcaggg atggatcacg cgccgcttcc agtgtttccc gctggctccg
    35461 ctgccggaat cactcagtca tcgcgtacca gacatcctgt atcccccgaa aacggagaaa
    35521 aaagaatgca aagacaatct gcaaccacac tgcttgcact ccttgtaggc tgtgcggtta
    35581 tccctgccac ccatgcctac tctgtgaatg ttgtgtccag tcagccggtc acccgacaga
    35641 tcatgccgga gctggccacc atacagggca tcatggagga aatggtccgt atgcagaccg
    35701 ccaccggcac agccatcacc cagaactcag aaaaactggc caccgtcatt gcccaggacg
    35761 ggcaggccac ccgtcagcag atgatattca gtaatgaaac acaccgcctc gaggaggcca
    35821 ggaaaagctt cagcgtgccg gactccatct gcagtgaatc cgcgtccggc attgcagcag
    35881 aaagccgccg ggcagcagcg tctgcagcgg cacgtctgag tcagggggga ggggtcagca
    35941 gtaaacccat ccgggaacgt ctctcccggg ctgcggactc cccggtccgc gaggcgtatg
    36001 acagtgccgg catccacgcc ggttactgca cggaagcgga gtatgcccgc tttggcggaa
    36061 ctgatgtctg ccctgccgtg ggcgatctgc cgggcggaga cagccaggta cgatcgctct
    36121 atcagggcgc aggcacagca gataccccgg ccgctctcac ctgggatcag aaacagattg
    36181 atgccgcgac ggcttatatg aaaaacaccg ccagaccgtc agcgggtcgt gccccgggga
    36241 aaggagaagt gggcacacag accgggcgca cgtatgtggg cctgcagaat gaatacaacg
    36301 gcatcatcga tgccgcctct catccgcagc tgtcactgat tgctgacagt acccctaatg
    36361 aagccacccg tggggcactg actgaagccc tgcagtcacc gtcagctgcg gcatattttg
    36421 accggaccgc atcatcagaa gcccgcaccc gtggccacat gagccagcgg gagtttgagg
    36481 cctttgaagc cggcagacgg tatgccaaca ccgactggca gcaggatctg cagggcatgg
    36541 aaggcgataa ccttctccgt gagctgctgc gcaccaccgc cctgctgaac tggcagatga
    36601 acgatctgaa agagcagatt cgtcagggta acgttatcgc cgggcagcaa cttgcgctgg
    36661 ccgcacggca gtattacgga cagcgccttg gcgaactgtc acaggccatg agccagggga
    36721 gcgtaagatg aaagacgaca acagcatcga cgaaaaaaaa ccggcccccc gtctccgccg
    36781 catcggtgat atagggataa atctgttcgc accactggcc gaaacccgct ggatgctgcg
    36841 cagctgccgc catatgctgg accagcataa aacgcgcctg aagcgcctca tgcccgaccg
    36901 gcaaccggag agtccggtct gtatgacctg ggaggaagcg gtcagggcca gcggactgac
    36961 ggtcgacgaa ctggatcggc gtctgcgacg tcgcaggatg ttctggcgga caggattctg
    37021 gctgatgatg ataccggtac ttctgctgtg tggtatggcc cttgcggcca ccgcactgcc
    37081 ggtcatcacc ctgatacgta tcctggtctg cctgttactg atgagtacca ttgcagcact
    37141 gtgtgcctca aaagccctga ttgcgtcata ccgcctctgg cagctgaatg aacgtcgggt
    37201 cagcgaggac gaacagggca cattccccga ttacctgcag gcccggcacg gctggaaaca
    37261 ggccacatgg ctggccatta cccaccggca gtactgactg tccggtctgc aatttctttt
    37321 cctcattccg ggggctgatg cccgggcatc cccttcggtt ccggagttac cgtgaaaatc
    37381 atacccagag cactgagtgc aggtctggcg ctggctgccg tacctgcaca ggctttcgtc
    37441 acctatcagg acattgtgag tgccgccacc aatccggatg acctgtcgcg ccaggcactg
    37501 gtcaccattt ttggtgatgt ggtcacaaat cccctgacag ccagcagcaa caccatcatt
    37561 ggcaacctgt tcatggtgtt taacgcaatt atcgccacga tggggatagt ctggtttctg
    37621 tttattggta tccgtcatgc tgtccgcacg ggacaccagg gcagtgtctt taacaacgga
    37681 cgggacgtcg tcggcatact cagtgtcgtg agtggatttt tgatgattgt gcccaccgct
    37741 tccggctgga gtctggcgca actggtcatg ctgtggggcg catccatcat gggcatcggt
    37801 tccgccaata tcatggttca gacagcggca gaaaacattg ctgaaggcta ttcaatgacc
    37861 gtacagccag tacatgtctc cacccgcacg gcagcacggg gaattctgga gatggaactg
    37921 tgcaaacacg ccatgaatac gggcctcgat gactttaacc gtaccgcacg ttcatcaacg
    37981 ccgcccatga gcgaatcctc acggacagag aatggcagat ataccgtcac cgtctcaaat
    38041 ggcagcgcca cctgcgggag tgtgagtctc tccgtgaaag gaaacggcag taacaagcag
    38101 tccgcgctgg cccatttttt caatcccttc attgaaacgg aatatcaggg ggttctgacg
    38161 gcacagcgct ccgccatgga taccatgctc cgtgacctgg acagcgcggc gcaggaattt
    38221 gtgtcggcat accggacccg gcgcgacacg ggtaacggca acctgccgga tattgaaagc
    38281 cgtattcaga aggcagccaa ccggtatgaa gaaacggtgc agaaggcgtt gccggcagat
    38341 aacgcagaaa ctgagcgcag ggagcagctg aaatcctacc tgaccaccta cggctgggtt
    38401 gccctgggcg cctggtacca gacctttgcc accgccaacc agcgactggc tgaactggcc
    38461 gaccgtgctc ctacagtgag tggcgtctcg tcactgggcg aagccgggga tacggaattc
    38521 ctgaaggaag tcaagggggc ttaccaggca cagctgcaga actcagcgta tactcctgca
    38581 ctgggaaccg ccacctctgc cagtgaatac cacgcatcta aaactcaaga ccctcaatca
    38641 gcaatcttaa aattgattgg gtttaaccta acaagaatga ctaactggac agccacttat
    38701 ctgagcggta caggtaccac aactaatcaa ctaaatccgt tgataaaaat gaaaaacatt
    38761 ggcgattaca ccatggttac gaccgaaacc atctgggcgg gttataccac cgtcagggta
    38821 ctttccacgg ccggcagcaa ctccgttttt ggtcgactgg tcaatgccgt cagcggatta
    38881 tctgacgccg caaaggcttt tctggaagca tccgcaccac cactctactt cctgctgttt
    38941 ttgctgtttt gtgccggttt ttcgttatcc atttacctgc cgtttatccc gtttatcttc
    39001 tggataaccg gcatcggcaa ctggatagtc agcgtactga ttggatgtgc tgctggcccc
    39061 ctgtgggccg ccacccatct gggaacaagt caggaccggg gaagccgagc cgcttacggt
    39121 tacatctacc tgatagacag catgatcaga ccgctgctga tggtgttcgg gttctttttt
    39181 gcatcagtcg ccatcgtggc cggcggcaca atcctgaatg cgctgtatgc tgctgcactg
    39241 atgaatgtcc agataaactc tgtgacgggt gtgttcagca tggcgggatt tttgatgatt
    39301 tatgcccgcg cctgtaccac actggttgca gccgtttttg cgcttcaggc ttatctgcct
    39361 gaccatgtaa ttaattttct gggcggacgt gacggtgtta atatgctggg gaatatggtt
    39421 tcttcagtca aagatattat tgccggaggt aataccaata tgcgcagaac acctggcgtt
    39481 cgtgaagaaa aactgaaaaa tattaataga gatgataaag acggaataaa acaataaatg
    39541 aaaatatcga aaaaccacta aaacaccatt gccatattca cctgaagaga ttaaaataca
    39601 ttcaggtgaa tatggaggtt tattatgaac acaacaaaaa gcagatatcc ttcctggtgg
    39661 gcatattatt accttatccg cactgcttat tttatatttg gtgttccttt tttattacta
    39721 tgttctctag gatttaccgg tatgatgctt acagaaccat atatagaaac agatattcaa
    39781 gcatatctat atacttatgg cagttggctc ctattaatac tcccatcact ctggctatat
    39841 atgaaaacca gagcgaagaa aaagaaagtc cagcaggttg ttcagcagat aaaagaaagc
    39901 agtccctttg cgccgaccag cgattatgag caactgtcat tctcgaagag ttgttatttc
    39961 gggatcgaca ttaaaaacgg caccatgctg tatgtgcgta tttatccaaa caatgtgatg
    40021 gatgtgattg gtctggatat tcacaacttt acccgcacgg ttgctgaaga cggcaagctg
    40081 gaaatccaca ccacctatgt gagtctgccg atgattcctc ttgagatctc cggcatctca
    40141 gcaaggacgc tggccaacac catgcacacc atggctgcac gtggatatga gtacaaggaa
    40201 cgtttcccac agatgatccg gtatcgggtt aaagagtggg aaaaagttgc cggtgttcct
    40261 gttgcagaag tattttgagg tagctatcaa ctgttagacg ataatccgtc agccggtttt
    40321 ctctgaaccg gctttttctt ttcaggcaga atgcctgtct gccgtctatc tcaggacgcc
    40381 gcatggagtc tctatgtttt ccccacaaat tcttagcact gtcttgccca ctgtagacaa
    40441 aatcaataca attctg
//
LOCUS       DAZOZD010000009        40113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOZD010000009 DAZOZD010000000
VERSION     DAZOZD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.009"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..77)
                     /locus_tag="ACHUPB_000374"
     tRNA            complement(1..77)
                     /locus_tag="ACHUPB_000374"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(81..173)
                     /locus_tag="ACHUPB_000375"
     tRNA            complement(81..173)
                     /locus_tag="ACHUPB_000375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Ser,seq:gct)
     gene            complement(489..674)
                     /gene="csrA"
                     /locus_tag="ACHUPB_000376"
     CDS             complement(489..674)
                     /gene="csrA"
                     /locus_tag="ACHUPB_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0757293.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(909..3539)
                     /gene="alaS"
                     /locus_tag="ACHUPB_000377"
     CDS             complement(909..3539)
                     /gene="alaS"
                     /locus_tag="ACHUPB_000377"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0757294.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3667..4167)
                     /gene="recX"
                     /locus_tag="ACHUPB_000378"
     CDS             complement(3667..4167)
                     /gene="recX"
                     /locus_tag="ACHUPB_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0757295.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4395..5456)
                     /gene="recA"
                     /locus_tag="ACHUPB_000379"
     CDS             complement(4395..5456)
                     /gene="recA"
                     /locus_tag="ACHUPB_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0757296.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="ACHUPB_000380"
     CDS             complement(5536..6033)
                     /gene="pncC"
                     /locus_tag="ACHUPB_000380"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0757297.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="ACHUPB_000381"
     CDS             complement(6178..7263)
                     /gene="mltB"
                     /locus_tag="ACHUPB_000381"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0757298.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7519..8082
                     /gene="srlA"
                     /locus_tag="ACHUPB_000382"
                     /gene_synonym="gutA"
     CDS             7519..8082
                     /gene="srlA"
                     /locus_tag="ACHUPB_000382"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR0757299.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFLHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8079..9038
                     /gene="srlE"
                     /locus_tag="ACHUPB_000383"
                     /gene_synonym="gutE"
     CDS             8079..9038
                     /gene="srlE"
                     /locus_tag="ACHUPB_000383"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0757300.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            9049..9420
                     /gene="srlB"
                     /locus_tag="ACHUPB_000384"
                     /gene_synonym="gutB"
     CDS             9049..9420
                     /gene="srlB"
                     /locus_tag="ACHUPB_000384"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0757301.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9424..10192
                     /gene="srlD"
                     /locus_tag="ACHUPB_000385"
                     /pseudo
     CDS             9424..10192
                     /gene="srlD"
                     /locus_tag="ACHUPB_000385"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10298..10657
                     /gene="gutM"
                     /locus_tag="ACHUPB_000386"
     CDS             10298..10657
                     /gene="gutM"
                     /locus_tag="ACHUPB_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0757302.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10724..11497
                     /gene="srlR"
                     /locus_tag="ACHUPB_000387"
                     /gene_synonym="gutR"
     CDS             10724..11497
                     /gene="srlR"
                     /locus_tag="ACHUPB_000387"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0757303.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11490..12455
                     /gene="gutQ"
                     /locus_tag="ACHUPB_000388"
     CDS             11490..12455
                     /gene="gutQ"
                     /locus_tag="ACHUPB_000388"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0757304.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12452..13966)
                     /gene="norR"
                     /locus_tag="ACHUPB_000389"
     CDS             complement(12452..13966)
                     /gene="norR"
                     /locus_tag="ACHUPB_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0757305.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14153..15592
                     /gene="norV"
                     /locus_tag="ACHUPB_000390"
     CDS             14153..15592
                     /gene="norV"
                     /locus_tag="ACHUPB_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0757306.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15589..16722
                     /gene="norW"
                     /locus_tag="ACHUPB_000391"
     CDS             15589..16722
                     /gene="norW"
                     /locus_tag="ACHUPB_000391"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0757307.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="ACHUPB_000392"
     CDS             complement(16850..19102)
                     /gene="hypF"
                     /locus_tag="ACHUPB_000392"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0757308.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="ACHUPB_000393"
     CDS             complement(19255..19782)
                     /gene="hydN"
                     /locus_tag="ACHUPB_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0757309.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="ACHUPB_000394"
     CDS             complement(19930..20940)
                     /gene="ascG"
                     /locus_tag="ACHUPB_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0757310.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21200..22657
                     /gene="ascF"
                     /locus_tag="ACHUPB_000395"
     CDS             21200..22657
                     /gene="ascF"
                     /locus_tag="ACHUPB_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR0757311.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22666..24090
                     /gene="ascB"
                     /locus_tag="ACHUPB_000396"
     CDS             22666..24090
                     /gene="ascB"
                     /locus_tag="ACHUPB_000396"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR0757312.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24204..24623)
                     /locus_tag="ACHUPB_000397"
     CDS             complement(24204..24623)
                     /locus_tag="ACHUPB_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0757313.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24620..24931)
                     /locus_tag="ACHUPB_000398"
     CDS             complement(24620..24931)
                     /locus_tag="ACHUPB_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR0757314.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25093..25866)
                     /locus_tag="ACHUPB_000399"
     CDS             complement(25093..25866)
                     /locus_tag="ACHUPB_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757315.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="ACHUPB_000400"
     CDS             complement(25891..26361)
                     /gene="hycI"
                     /locus_tag="ACHUPB_000400"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR0757316.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="ACHUPB_000401"
     CDS             complement(26354..26764)
                     /gene="hycH"
                     /locus_tag="ACHUPB_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR0757317.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="ACHUPB_000402"
     CDS             complement(26761..27528)
                     /gene="hycG"
                     /locus_tag="ACHUPB_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR0757318.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="ACHUPB_000403"
     CDS             complement(27528..28070)
                     /gene="hycF"
                     /locus_tag="ACHUPB_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR0757319.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="ACHUPB_000404"
     CDS             complement(28080..29777)
                     /gene="hycE"
                     /locus_tag="ACHUPB_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0757320.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="ACHUPB_000405"
     CDS             complement(29795..30718)
                     /gene="hycD"
                     /locus_tag="ACHUPB_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0757321.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="ACHUPB_000406"
     CDS             complement(30721..32547)
                     /gene="hycC"
                     /locus_tag="ACHUPB_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR0757322.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="ACHUPB_000407"
     CDS             complement(32544..33155)
                     /gene="hycB"
                     /locus_tag="ACHUPB_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0757323.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="ACHUPB_000408"
     CDS             complement(33280..33741)
                     /gene="hycA"
                     /locus_tag="ACHUPB_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0757324.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33953..34303
                     /gene="hypA"
                     /locus_tag="ACHUPB_000409"
     CDS             33953..34303
                     /gene="hypA"
                     /locus_tag="ACHUPB_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0757325.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34307..35179
                     /gene="hypB"
                     /locus_tag="ACHUPB_000410"
     CDS             34307..35179
                     /gene="hypB"
                     /locus_tag="ACHUPB_000410"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0757326.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35170..35442
                     /gene="hypC"
                     /locus_tag="ACHUPB_000411"
     CDS             35170..35442
                     /gene="hypC"
                     /locus_tag="ACHUPB_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0757327.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35442..36563
                     /gene="hypD"
                     /locus_tag="ACHUPB_000412"
     CDS             35442..36563
                     /gene="hypD"
                     /locus_tag="ACHUPB_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0757328.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36560..37570
                     /gene="hypE"
                     /locus_tag="ACHUPB_000413"
     CDS             36560..37570
                     /gene="hypE"
                     /locus_tag="ACHUPB_000413"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0757329.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37644..39721
                     /gene="flhA"
                     /locus_tag="ACHUPB_000414"
                     /pseudo
     CDS             37644..39721
                     /gene="flhA"
                     /locus_tag="ACHUPB_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39758..40111)
                     /locus_tag="ACHUPB_000415"
     CDS             complement(39758..40111)
                     /locus_tag="ACHUPB_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0757330.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         9457 a        11058 c        10425 g         9173 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcaaa tggcggtgag gcggggattc gaaccccgga tgcagctttt
      121 gaccgcatac tcccttagca ggggagcgcc ttcagcctct cggccacctc accacacgcc
      181 tcttacgagt gcttcgaaga acttgtttat cgctcatcgt cgctgcgtgg cgcacatatt
      241 actttctgag acttataagt caaacaattt ttcccacact tttatcgttt gcacacttca
      301 cgctcaatta gtctgtaaaa acgtcaaaaa gagtgtttta tctacagaaa aacggaggtc
      361 tggcagatag cagtaatgca aaaaaatgga gacttaagtt gaatgaacgg gagtaaagcg
      421 aaaagactat agagtgaagg agaaattttg agggtgcgtc tcaccgataa agatgagacg
      481 cggaaagatt agtaactgga ctgctgggat ttttcagcct ggatacgctg gtagatctct
      541 tcacggtgaa cagaaacttc cttcggggca tttacgccaa tacgtacctg gttgcccttt
      601 acccctaaaa ctgtcacggt gacctcatcc ccaatcatga gggtctcacc aactcgacga
      661 gtcagaatca gcattctttg ctccttgaaa gattaaaaga gtcgggtctc tctgtatccc
      721 ggcattatcc atcatataac gccaaaaagt aagcaatgac aaacacatta catgtaagca
      781 gtcatggcat tacattctgt taaacctaag tttagccgat atacacaact tcaacctgac
      841 tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc gagtccacgg cattgcctga
      901 cgcttatatt attgcaattt cgcgctgacc cagcctttca cactggctaa cgctgcaggt
      961 aaggccgcag catccgtacc accggcttgc gccatgtcag gacgtccacc acccttgccg
     1021 cccacctgct gagcgaccat accaatcagt tcccctgctt tcacacgatc tgtgacgtcc
     1081 ttagatacgc ctgcaatcag agaaacctta ccttcggcta ccgttgccag cacgataatt
     1141 gtcgacccca gctgattttt taaatcgtca accatggtac gcaacatttt cggctcaaca
     1201 ccgctaagct cgctaaccaa cagcttaaca ccattaacat caattgcctt actggaaaga
     1261 tttgcgctct cctgtgcggc agcttgttct ttaagctgtt gtagttcttt ttctaactga
     1321 cgcgtacgtt ccagtactga gcgcacttta tccgccagat tattgctatc gcctttcagc
     1381 agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa cggtggtgat agcgccttct
     1441 ccggttaccg cttcgatacg acgaacgcct gcagcagtac ccgattcaga gatgatgcgg
     1501 aacagaccaa tatcaccagt gcggctggcg tgagtaccgc cacacagctc ggtggagaaa
     1561 tcgcccatgc tcagcacgcg tacacgctca tcatacttct cgccgaacag cgccatcgca
     1621 cctttcgctt tcgccgcttc gagatccatg atgttggttt cgatcggcaa attgcgacga
     1681 atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt ccggtttcat cgcttcgttg
     1741 tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg aacctttctg cgatacatga
     1801 gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg ttgcggagtg attcagacga
     1861 atacgggcgc gacgagcctc atcaacatca gcctgcacag cgtcgcccac tttcagagaa
     1921 cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt atttctgcgt atcttccacc
     1981 gcgaaggaga agttagcgcc tttcagttcg cctttatcac caacctgacc gccggattcc
     2041 gcatagaatg gcgtttgatc cagcacgacc acagcttcct ggcctgcatt gatggcatca
     2101 accgctttac catcaacaaa cagcgcggtc actttgccgt tcagttccag atggtcatag
     2161 cctttaaatt cagatgcact atcaacacgg atcattgcgt tgtaatcggc accaaagccg
     2221 ctggcttcgc gcgcgcgacg acgctgctct tccattgctg cttcaaaacc agcttcgtca
     2281 actttgatgt tgcgctcacg acaaacatca gccgtcaggt caaccgggaa gccataggtg
     2341 tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac cagaaagttt tgccagctct
     2401 tcatccagca acgccagacc gcgctccaga gtacgggcaa actgctcttc ttcagtcttc
     2461 agcacctgct caacctgcgc ctgctggcgt ttcaggtctt cacccgcaga gcccataacg
     2521 tcgatcagcg gaccaaccag tttgtagaag aaggtttctt tcgcgccgag catattgccg
     2581 tgacgcactg cgcgacgaat gatacgacgc agtacataac cacggttttc attggacggc
     2641 attacgccat ccgcgatcag gaacgcacaa gaacgaatgt ggtcagcgat tacgcgcagc
     2701 gatttattgc tcagatcggt cgcgccagtg actttcgcta ccgcctggat caacgtgcgg
     2761 aacaggtcga tgtcatagtt agagttaacg tgttgcagca ccgcagcaat acgctccaga
     2821 cccataccgg tatctacaga cggcttcggc agcggttcca tcgtgccatc tgcctggcgg
     2881 ttgaactgca tgaagacgat gttccagatc tcaatgtagc ggtcgccgtc ttcttccgga
     2941 cttcccggag ggccacccca aatgtggtcg ccgtgatcgt agaagatttc ggtgcacggg
     3001 ccgcacggac cagtgtcacc catctgccag aagttgtcag atgcgtatgg cgcacctttg
     3061 ttatcgccga tgcgaataat acgttcgcgc gggatcccta cttctttttc ccagatttcg
     3121 taggcttcgt cgtcgctttc atagacggta acccacagac gctctttcgg cagggcaaac
     3181 catttttcgc tggtcagcag ttcccatgca aactgaatgg catcgtgttt gaaatagtcg
     3241 ccgaagctga agttgcccag catttcgaag aaggtatggt gacgcgcggt gtaaccgaca
     3301 ttttccaggt cgttgtgttt accacccgca cgcacgcagc gttgggaagt ggtagcgcgg
     3361 gaataattac gcttgtcgag tccaaggaac acatccttga actggttcat cccggcgttg
     3421 gtaaacaaca aagttgggtc gttatggggt accagggagc tgctggcaac tacctgatgt
     3481 cccttactat ggaaaaagtc gagaaacgcc tgacggatct cagcggtgct cttgctcata
     3541 attatcctga aatcaagcta acgaaatatc gccaccagct ccagcgtgtc ttaaccgccg
     3601 ggctggtaac tgaaaagtgg gaataagata agttttcttg agtgggaagt aaaataccgt
     3661 atgcgttcag tcggcaaaat ttagccaaat atcctggata tcttccatca gatagccacg
     3721 atagagcaga aaacgctgga tcttaacttt ttctgaaaag acagttggca aaggttcacc
     3781 atattttcgc gtcgcctgat cgcgcaccag tgcgcaccag tcgatgtcac attcacgcat
     3841 cgctttttct gtcgcttcgc gggaaatacc tttctgattc agttcctgac gaatacgtgc
     3901 aggtccataa cctttgcggc tacggctggc gataaagcgc gcaacaaatc ggctgtcatc
     3961 gagatagcca tgttcatggc accaggcaat aacgcgctcg taatcttctg ccgtagcatc
     4021 aatctcttct gggccatttt tgcccataat cggtgccgcg agtttacgtc gcagttcttg
     4081 ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga tccaacaggc gagcatatgc
     4141 cgggcgacgg gatgttgatt ctgtcatggc atatccttac aacttaaaaa agcaaaaggg
     4201 ccgcagatgc gacccaggtt ttgctgataa acagatttaa tagtcaatga gtgtgcagcc
     4261 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgttggcc tgttcccctc
     4321 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
     4381 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
     4441 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
     4501 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
     4561 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
     4621 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
     4681 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
     4741 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
     4801 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
     4861 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
     4921 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
     4981 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
     5041 actgcgccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
     5101 tgggagcaca gcaggttatc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
     5161 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
     5221 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
     5281 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
     5341 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
     5401 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
     5461 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
     5521 tatactcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
     5581 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
     5641 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
     5701 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
     5761 cagtgcacct atcgccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
     5821 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
     5881 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
     5941 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
     6001 ttcgcttaac tgcatcagtt cactgtcagt catcacaacc tcagtcagtc aaaaatttat
     6061 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
     6121 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
     6181 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
     6241 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
     6301 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
     6361 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
     6421 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
     6481 tttgaaatag ttcgccacac taccgatcgc atcaaccgga tcccacaggt tgatatgccc
     6541 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
     6601 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
     6661 caacaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
     6721 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
     6781 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
     6841 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
     6901 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
     6961 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
     7021 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattctgt cgatgaactg
     7081 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgaggctc
     7141 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
     7201 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
     7261 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
     7321 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
     7381 gctatccttt tattcaaact ttcaaattaa aatatttatc tttcattttg cgatcaaaat
     7441 aacactttca aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
     7501 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
     7561 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
     7621 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
     7681 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
     7741 ggcacgtttg tcttttgcaa tccgatgacc ttaagcctgg gtcgctttat gccggaaaag
     7801 tacaaaccca gctactacgc ggcagcctct tatagctgcc actcaatgaa tggcctcttt
     7861 ctccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
     7921 ctgaacctgc cacttggccc actggcggtg agctatctgc tggttggtct ggtcaccaac
     7981 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
     8041 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
     8101 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
     8161 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
     8221 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
     8281 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
     8341 ttaatatcca ctcgacgggc aagtccagtc cgctggcgca gtacattgtg gaagatattt
     8401 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
     8461 cttccgtggg ccgtgactat gacaccagca agaaaatcac cgaacaaagc gatggtttac
     8521 tggcgaaggt gggaatgggt atgggttctg ccgttgccgt gttgtttcaa tctggtcgtg
     8581 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcgttcgtc tcggcgctca
     8641 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
     8701 cgagccatcc actaggtctg gtcatgctgg cgctcatctg ctccttcccg ctgctttcac
     8761 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
     8821 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
     8881 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
     8941 ttcgcgtcgg tgtcccttct gtactggtga gccacttttt aaccggcgca ccgactgtac
     9001 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
     9061 tatcagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
     9121 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
     9181 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
     9241 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
     9301 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
     9361 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
     9421 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
     9481 cacggtctgg ctgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
     9541 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaag gaacagcgta tagttttggt
     9601 gctgacgcca ccagcgagca aagcgtcctg gcgctctctc gtggggtaga tgaaatcttt
     9661 gggcgcgtgg atttgctggt ctacagcgcc ggaatagcaa aagcagcctt tatcagcgac
     9721 ttccagctcg gcgattttga ccgtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
     9781 ttctcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
     9841 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
     9901 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
     9961 gctcggtaac ctgctgaaat cgcctatgtt ccagtcactg ttaccccaat acgcgaccaa
    10021 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10081 cggctgcgat tatcaggatg tgctgaatat gctgctgctc tatgccagtc ctaaggcgtc
    10141 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttgt aatcaacagc
    10201 ggagatccgt taaggatctc cgtgagacta atagaatgcc tgatgcgcta cgctcatcag
    10261 gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac tcatcaccgt
    10321 cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt ctcgttttaa
    10381 ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg
    10441 ctttaaacca cgggtcgtgg tcgccatcgc cctggacgat cagcagcgca tcgtcgacac
    10501 cttgtttatg aaaggactga ccgtcttcgc acgaccgcaa aaaattcccg caattaccgg
    10561 tatgcatgcg ggtgatttac agcccgatgt gatctttccc catgatccac tatcacagaa
    10621 tgctctatca ttggcgctta aactgaaacg tggataattt cgttgtgaat gttacttgct
    10681 tgcgaagtta tcattttgaa acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg
    10741 tcaggccgcc attctggagt atctgcaaaa gcagggtaaa tgctcggttg aagaattggc
    10801 gcaatacttt gacaccacag gcacaaccat tcgcaaagat ctggtcattc tggaacatgc
    10861 aggaaccgtc attcgtactt atggcggagt ggtgttgaat aaagaggaat ccgatccgcc
    10921 tatcgatcat aaaacactca tcaacaccca caagaaagag ctgatagcag aagctgccgt
    10981 tagttttatt catgatggcg attcgatcat tcttgatgct ggcagtaccg ttttgcagat
    11041 ggttcccctg ctctcgcgct ttaataacat cacggtgatg accaacagtc tgcatatcgt
    11101 caatgcgcta tctgaactgg ataacgaaca aactatcctg atgccaggcg gaacgtttcg
    11161 taaaaaatcg gcctcatttc acggacagct ggcagagaat gccttcgaac atttcagttt
    11221 cgataaattg tttatgggca ccgacggcat cgatctcaat gcgggcgtaa ccacctttaa
    11281 cgaggtttat accgtcagta aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc
    11341 ggactcatca aagtttggcc gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga
    11401 taagctgatt accgacgcag gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg
    11461 gatcgatgtg atcataaccg gagagagcaa tgagtgaagc actactgaac gcgggacgtc
    11521 agacgttaat gctggagttg caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt
    11581 ttgttcgcgc cgccaatatc atcctgcact gcgaaggcaa agtggtggtt tcgggaattg
    11641 gcaaatcggg ccacattggt aagaaaatcg ccgcaacact tgccagtacc ggcacatcgg
    11701 ctttttttgt ccatccggca gaagcactgc acggcgatct ggggatgatt gaaagccgcg
    11761 atgtgatgct gtttatctct tactccggtg gcgcgaagga actggatctg attattccgc
    11821 gtctggaaga taaatctatc gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg
    11881 gcctggcggc aaaagcggtg ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc
    11941 ttgcgccgac ctccagcacc gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg
    12001 tcatgcaggc gcgcggattt aatgaagaag attttgcccg ctcccaccca gccggggcac
    12061 tgggcgctcg cttgctgaat aaagtgcatc atctgatgcg ccgtgacgat gccatcccac
    12121 aggtggcgtt aaccgccagc gtgatggatg cgatgctgga actcagccgc accggtctgg
    12181 ggctggtggc ggtatgtgac gctcaacaac aggtacaagg cgtctttacc gacggcgatt
    12241 tacgtcgctg gctggtaggc ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga
    12301 caggcggcac cacgttacag gcgcaaagtc gcgccatcga cgccaaagag atcctgatga
    12361 agcgcaaaat cactgccgca ccggtggtgg atgaaaacgg caaactcacc ggcgcaatta
    12421 acctgcagga tttctatcag gccgggatta tttaatcctt cattcccaga cgtttcgcca
    12481 gccgatgcag gttggcgacg tcggtttcca gcatccgcgc gcaggcagcc cagttatgat
    12541 gattttgtgc cagtgcctgg cgaatagttt cacgctggaa cgcttctgtc gcttcacgca
    12601 ggttttgctt aacaacgggc accgccgccg cttctggcgg cggcaacgtc acctcaggaa
    12661 aagcaaaatg ttgcgcctca agaatcactt catcgccgtt gcgggtggct ctcgccagca
    12721 ctaccgcccg atgaatagca tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt
    12781 gcagtaaatt tcgcgctccg gcacttaata ccacgcggga gagccccagc cgcaaacgac
    12841 actgctcgca gaaatacccc gccagcagaa tgacatcatc gccccgctca cgcagcggcg
    12901 gcaccgaaag tggaaacacg ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca
    12961 tcacctcttc gcgtaagtcg cggttagtcg ccgccagcac gcgcacatcg acccgcaaac
    13021 tacggtcatc gccaacgcgc tgaatatcgc catactgcaa caccctcagc agcttggcct
    13081 gcaatgccaa cgacaactcg ccgatctcat ccagaaacag agtgccgtta tccgccattt
    13141 caaacttccc gctgcggtta ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca
    13201 actcactttc cgccacactt tccggcagtg cagcacagtt gagatagacc agcggattca
    13261 ccgcccgtgg cgaggcttca tgaatcgctt tcgccaccag ctccttaccg gttcccgtct
    13321 caccgctgat caggacgttg agatcggacg ccgccacaat ctcaatctct tttttcagtt
    13381 gcgtcatgcc aggggacaag ccaatcatct gcgtctgttt caccgcttca aacggcgtgg
    13441 catcgcctgg catcatattc tggctttcca gttgttcaat cagcaacgca ttgcttaacg
    13501 ctcccgccgc cagcgcagca atcagccgta gctcttcgtc gctgaaaaca tcgaactgat
    13561 cgggctgcat cccgtcgagc gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca
    13621 gaccaacgca ggcgtgaacc ttcagactct cctgcccagg aatcaaaccg tcatagggat
    13681 cgggcaattc gctgtctgcg ggaaagcgca ccacatcccc ggcgcgggca atcgcttcca
    13741 gccgtggatg cccttccagc gcaaagcgtc taccgagtac atcctttgcc agaccgtcga
    13801 tggcaagcgg aataaactgc cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca
    13861 gcacctgacg tagcgtggtg atcaggcgct gaaaacgatc ctgatgacca atcccacgct
    13921 gcaattcgat ggcgatattc gccagcacat caacggaaaa actcatcttt gcctcactgt
    13981 caatttgact atagatattg tcatatcgac catttgattg atagtcattt tgactactca
    14041 ttaatgagca taattttatt tatagagtaa aaacaatcag ataaaaaact ggcacgtaat
    14101 ctgcaattag caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat
    14161 tgtggtgaaa aataacattc attgggttgg tcaacgtgac tgggaagtgc gtgattttca
    14221 cggtacggaa tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga
    14281 aaaaaacgtg ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct
    14341 gcgtaatgaa atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga
    14401 ccacgctggg gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac
    14461 tgccaacgct atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt
    14521 ggtgaaaact ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac
    14581 gccaatgctg cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt
    14641 cagtaacgat gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga
    14701 tcagacggag cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag
    14761 ccgcctggta acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat
    14821 agcgacctct cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct
    14881 gaaatgggcg gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa
    14941 taacacccgc atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt
    15001 ggcggtgaaa attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt
    15061 ccgctcaaaa ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat
    15121 cgccggtctg gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt
    15181 cggctctcac ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc
    15241 gggtttcgaa atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga
    15301 gttatgccgt gaacatggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca
    15361 gagcacggtg aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg
    15421 cccacggatg cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat
    15481 gcaggacgtt gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga
    15541 atgctctctc ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc
    15601 attgtgatca ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag
    15661 gacgccagta ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg
    15721 gacctcagcc atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg
    15781 ggtgaatttg ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc
    15841 gatgccgaag cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg
    15901 ctggcaactg gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg
    15961 ttaaatagtc agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg
    16021 ttgattgtcg gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc
    16081 aaagcggtca cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa
    16141 gtaagcagcc gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct
    16201 cagttgcagg ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt
    16261 agtatcgaag tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca
    16321 cgacgcgccg ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt
    16381 aatgacgata tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc
    16441 ctgcagccga ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg
    16501 ccgctgaaac taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg
    16561 gcaggcgaaa cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg
    16621 gtggctcgcg gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg
    16681 atgaaagagg catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa
    16741 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccag
    16801 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    16861 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    16921 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    16981 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    17041 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    17101 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg
    17161 cgagatccag ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg
    17221 aggctgcgag cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    17281 cacagcccag tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg
    17341 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    17401 ctgtttcagg gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    17461 gccacggctg cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    17521 ggtgctcaca ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct
    17581 cccccatacc gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact
    17641 gatgctctgc cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca
    17701 gattcatttc acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt
    17761 attgcggggt aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    17821 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    17881 ccaggcagaa ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa
    17941 agcccggagg caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc
    18001 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    18061 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    18121 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    18181 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    18241 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    18301 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    18361 tcgccgggcg atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc
    18421 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    18481 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    18541 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    18601 cacggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    18661 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    18721 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag
    18781 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cggatggtga
    18841 actcagtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    18901 gcggcgggca gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga
    18961 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    19021 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    19081 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc
    19141 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    19201 ttctacgaat atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa
    19261 catcagcgcc gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac
    19321 acaaatcagc gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt
    19381 gcacaggtca catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct
    19441 gttgcgaatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca
    19501 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    19561 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc
    19621 cgtggaaatg ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag
    19681 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacagca
    19741 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    19801 cagttaatgc gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga
    19861 aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga
    19921 tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa
    19981 ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc
    20041 ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc
    20101 aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc
    20161 gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa
    20221 tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt
    20281 accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata
    20341 cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc
    20401 ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca
    20461 ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact
    20521 gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat
    20581 ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg
    20641 tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa
    20701 gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt
    20761 ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc
    20821 taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac
    20881 gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat
    20941 cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt
    21001 gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa aatgaacaat
    21061 taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt
    21121 ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact
    21181 gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc
    21241 ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt
    21301 tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct
    21361 cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc
    21421 ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa
    21481 acccaaactc acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat
    21541 gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct
    21601 cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg
    21661 tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa
    21721 aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga
    21781 actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc
    21841 ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat
    21901 cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct
    21961 gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat
    22021 cggtagcgcc atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt
    22081 cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac
    22141 gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga
    22201 gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa
    22261 cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc
    22321 cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat
    22381 cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc
    22441 agcgccaggg ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt
    22501 ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct
    22561 cggctttgag gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt
    22621 acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca
    22681 gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa
    22741 ggtggcaaag ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg
    22801 aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag
    22861 gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc
    22921 aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg
    22981 cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt
    23041 atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat
    23101 ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt
    23161 gaagcatttg atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg
    23221 catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg
    23281 aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat
    23341 gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac
    23401 agttgcaagc cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt
    23461 atcgatgtgc aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa
    23521 ggggtaacca tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt
    23581 gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc
    23641 agtgcggcga acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc
    23701 tggggtattg atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag
    23761 aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc
    23821 gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa
    23881 gcgattgcag atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc
    23941 tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac
    24001 gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg
    24061 attgccagta atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct
    24121 tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat
    24181 ctggggtttg gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct
    24241 gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc
    24301 agggatttag agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc
    24361 agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca
    24421 aactcttggt tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga
    24481 ttggtgtcat cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct
    24541 tctgagtcac tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca
    24601 gcgcgtgcag cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat
    24661 actcggcgtg attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg
    24721 caatcaccct taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa
    24781 aattgtcgag gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct
    24841 taataacccg atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact
    24901 cttcaaatgg cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt
    24961 aaacagccac atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc
    25021 aagttcacaa tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca
    25081 ctctgcaaaa agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg
    25141 ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag
    25201 tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca
    25261 gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc
    25321 ggaatcaaac tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc
    25381 atctacgcca gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc
    25441 ttcgcgcgtg aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact
    25501 tggtaaccag ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg
    25561 ccctttgccc gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg
    25621 atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc
    25681 agcaacgccc acttttctaa aatgccaggt ttgctcggca tcataaggca tggtcaggag
    25741 aggtttcagc gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa
    25801 gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat
    25861 attcatttcg ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa
    25921 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    25981 cggctgagtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    26041 ttcgccaatg tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt
    26101 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag
    26161 ccccatatca gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    26221 gatgtcgttt tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc
    26281 ggcgcacttt tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac
    26341 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    26401 gaatatcatg cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg
    26461 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    26521 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    26581 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    26641 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    26701 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    26761 tcatcggata cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg
    26821 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    26881 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    26941 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc
    27001 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    27061 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg
    27121 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaaaatac caccgctgtt
    27181 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    27241 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    27301 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    27361 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga
    27421 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    27481 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    27541 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    27601 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    27661 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg
    27721 cagttgcaca gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt
    27781 tcgtactctt gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    27841 aagatgcagc gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca
    27901 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc
    27961 gggttctgct ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    28021 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    28081 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    28141 actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    28201 gggtccaagc taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag
    28261 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    28321 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    28381 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta
    28441 tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga
    28501 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg
    28561 acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca
    28621 acgttactga aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt
    28681 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    28741 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    28801 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag
    28861 gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg
    28921 agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    28981 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg
    29041 gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg
    29101 taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag
    29161 tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    29221 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    29281 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    29341 ttacgcagcg gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc
    29401 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    29461 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    29521 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    29581 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    29641 accacttccg gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag
    29701 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    29761 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    29821 ctaaaaatgc aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac
    29881 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    29941 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    30001 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    30061 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    30121 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    30181 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    30241 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    30301 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    30361 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    30421 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    30481 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    30541 aggcatccgg gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca
    30601 acacacccgg cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    30661 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    30721 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    30781 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    30841 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    30901 agcggtaatc accattgatt tttcatggtc gtagtcgcac acccaggccg caccgcggga
    30961 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    31021 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    31081 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    31141 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    31201 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    31261 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    31321 ggcaacaaac gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc
    31381 ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    31441 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    31501 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    31561 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    31621 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    31681 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    31741 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag
    31801 tgacagggtt aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    31861 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    31921 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    31981 ttgcatacgc atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca
    32041 gcaagcaatc accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    32101 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    32161 catgccgagg ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg
    32221 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    32281 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc
    32341 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc
    32401 cgcggctgcc gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa
    32461 cgctttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc
    32521 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    32581 gtgagatcgc caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg
    32641 atatcggtgt tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga
    32701 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    32761 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    32821 ttggcgtttg ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    32881 caggcgatgc cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca
    32941 tcaatgcggg tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg
    33001 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    33061 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    33121 cagagcgtgg agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    33181 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    33241 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    33301 ctttaccagt tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc
    33361 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    33421 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    33481 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    33541 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    33601 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    33661 gtagatgtgc cactggtcct gtaggcgaca atgccgctgt gcgatgtaat cggctttctc
    33721 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    33781 attttctatg ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat
    33841 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    33901 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    33961 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    34021 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    34081 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    34141 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    34201 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    34261 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    34321 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    34381 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    34441 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    34501 gggcactcac gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    34561 ggacaaaaat aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    34621 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct
    34681 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    34741 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    34801 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    34861 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    34921 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    34981 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    35041 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    35101 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    35161 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    35221 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    35281 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    35341 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    35401 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    35461 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    35521 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    35581 ccatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    35641 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    35701 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg
    35761 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    35821 atgcgttgaa actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt
    35881 ttgaaaccac catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc
    35941 agaattttta cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg
    36001 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    36061 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    36121 tcgaacccct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    36181 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    36241 aacaggcaat tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    36301 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    36361 atttccgccc agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    36421 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    36481 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    36541 gtcagcagga gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    36601 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    36661 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc
    36721 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    36781 gctggcgatt tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    36841 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    36901 cagcatggca gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt
    36961 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    37021 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    37081 cacgctcggg gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    37141 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    37201 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    37261 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc
    37321 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    37381 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    37441 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    37501 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg
    37561 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    37621 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    37681 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    37741 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    37801 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata
    37861 tgaagacgaa actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac
    37921 gctgcattgc agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct
    37981 ataccccaaa tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg
    38041 tggctgtgaa tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct
    38101 gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa
    38161 caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat
    38221 taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga
    38281 aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa
    38341 caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag
    38401 cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct
    38461 gatcaatctc cacgaacggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta
    38521 gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg
    38581 cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct
    38641 gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat
    38701 ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa
    38761 tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa
    38821 acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac
    38881 gggtaaagag ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat
    38941 ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca
    39001 tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga
    39061 taaaagctct ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt
    39121 gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac
    39181 ggacgtacgt ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga
    39241 gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg
    39301 cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg
    39361 tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga
    39421 gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg
    39481 cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc
    39541 tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct
    39601 gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa
    39661 acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta
    39721 actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac
    39781 gatatttttc cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta
    39841 agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt
    39901 cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc
    39961 cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg
    40021 gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca
    40081 gtttttcacg agagatacgc ttgccggaca tgc
//
LOCUS       DAZOZD010000010        38592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOZD010000010 DAZOZD010000000
VERSION     DAZOZD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.010"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            7..1056
                     /locus_tag="ACHUPB_000416"
     CDS             7..1056
                     /locus_tag="ACHUPB_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0757331.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1130..1987
                     /gene="nfo"
                     /locus_tag="ACHUPB_000417"
     CDS             1130..1987
                     /gene="nfo"
                     /locus_tag="ACHUPB_000417"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0757332.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1991..3079
                     /locus_tag="ACHUPB_000418"
     CDS             1991..3079
                     /locus_tag="ACHUPB_000418"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0757333.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3135..4385)
                     /locus_tag="ACHUPB_000419"
     CDS             complement(3135..4385)
                     /locus_tag="ACHUPB_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0757334.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4485..5426)
                     /gene="rihB"
                     /locus_tag="ACHUPB_000420"
     CDS             complement(4485..5426)
                     /gene="rihB"
                     /locus_tag="ACHUPB_000420"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0757335.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5556..6254
                     /locus_tag="ACHUPB_000421"
     CDS             5556..6254
                     /locus_tag="ACHUPB_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR0757336.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLLDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6321..7571)
                     /locus_tag="ACHUPB_000422"
     CDS             complement(6321..7571)
                     /locus_tag="ACHUPB_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0757337.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7665..8603)
                     /gene="psuG"
                     /locus_tag="ACHUPB_000423"
     CDS             complement(7665..8603)
                     /gene="psuG"
                     /locus_tag="ACHUPB_000423"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0757338.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8591..9532)
                     /locus_tag="ACHUPB_000424"
     CDS             complement(8591..9532)
                     /locus_tag="ACHUPB_000424"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0757339.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(9990..11681)
                     /gene="fruA"
                     /locus_tag="ACHUPB_000425"
     CDS             complement(9990..11681)
                     /gene="fruA"
                     /locus_tag="ACHUPB_000425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0757340.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11698..12636)
                     /gene="fruK"
                     /locus_tag="ACHUPB_000426"
     CDS             complement(11698..12636)
                     /gene="fruK"
                     /locus_tag="ACHUPB_000426"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0757341.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12636..13766)
                     /gene="fruB"
                     /locus_tag="ACHUPB_000427"
     CDS             complement(12636..13766)
                     /gene="fruB"
                     /locus_tag="ACHUPB_000427"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0757342.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14134..15315
                     /gene="setB"
                     /locus_tag="ACHUPB_000428"
     CDS             14134..15315
                     /gene="setB"
                     /locus_tag="ACHUPB_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0757343.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15312..15566)
                     /locus_tag="ACHUPB_000429"
     CDS             complement(15312..15566)
                     /locus_tag="ACHUPB_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0757344.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15721..16293
                     /gene="yeiP"
                     /locus_tag="ACHUPB_000430"
     CDS             15721..16293
                     /gene="yeiP"
                     /locus_tag="ACHUPB_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0757345.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16516..17982
                     /locus_tag="ACHUPB_000431"
     CDS             16516..17982
                     /locus_tag="ACHUPB_000431"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0757346.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18100..19086
                     /gene="yeiR"
                     /locus_tag="ACHUPB_000432"
     CDS             18100..19086
                     /gene="yeiR"
                     /locus_tag="ACHUPB_000432"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0757347.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19125..19838
                     /gene="lpxT"
                     /locus_tag="ACHUPB_000433"
     CDS             19125..19838
                     /gene="lpxT"
                     /locus_tag="ACHUPB_000433"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0757348.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20250..20816
                     /gene="mepS"
                     /locus_tag="ACHUPB_000434"
     CDS             20250..20816
                     /gene="mepS"
                     /locus_tag="ACHUPB_000434"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0757349.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            20997..22553
                     /locus_tag="ACHUPB_000435"
     CDS             20997..22553
                     /locus_tag="ACHUPB_000435"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0757350.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22635..24449
                     /locus_tag="ACHUPB_000436"
     CDS             22635..24449
                     /locus_tag="ACHUPB_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0757351.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24450..25544
                     /locus_tag="ACHUPB_000437"
     CDS             24450..25544
                     /locus_tag="ACHUPB_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0757352.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25544..26569
                     /locus_tag="ACHUPB_000438"
     CDS             25544..26569
                     /locus_tag="ACHUPB_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0757353.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26571..28160
                     /gene="yejF"
                     /locus_tag="ACHUPB_000439"
     CDS             26571..28160
                     /gene="yejF"
                     /locus_tag="ACHUPB_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0757354.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28164..28508)
                     /locus_tag="ACHUPB_000440"
     CDS             complement(28164..28508)
                     /locus_tag="ACHUPB_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0757355.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28841..29898)
                     /locus_tag="ACHUPB_000441"
                     /pseudo
     CDS             complement(28841..29898)
                     /locus_tag="ACHUPB_000441"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(29989..30684)
                     /gene="rsuA"
                     /locus_tag="ACHUPB_000442"
     CDS             complement(29989..30684)
                     /gene="rsuA"
                     /locus_tag="ACHUPB_000442"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0757356.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30834..32594
                     /locus_tag="ACHUPB_000443"
     CDS             30834..32594
                     /locus_tag="ACHUPB_000443"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0757357.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32719..33003
                     /gene="rplY"
                     /locus_tag="ACHUPB_000444"
     CDS             32719..33003
                     /gene="rplY"
                     /locus_tag="ACHUPB_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0757358.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33142..34149)
                     /gene="yejK"
                     /locus_tag="ACHUPB_000445"
     CDS             complement(33142..34149)
                     /gene="yejK"
                     /locus_tag="ACHUPB_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0757359.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34331..34558
                     /locus_tag="ACHUPB_000446"
     CDS             34331..34558
                     /locus_tag="ACHUPB_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0757360.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34578..36338
                     /gene="yejM"
                     /locus_tag="ACHUPB_000447"
     CDS             34578..36338
                     /gene="yejM"
                     /locus_tag="ACHUPB_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0757361.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36413..36489
                     /locus_tag="ACHUPB_000448"
     tRNA            36413..36489
                     /locus_tag="ACHUPB_000448"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36447..36449,aa:Pro,seq:ggg)
     gene            complement(36592..38592)
                     /gene="yejO"
                     /locus_tag="ACHUPB_000449"
                     /pseudo
     CDS             complement(36592..38592)
                     /gene="yejO"
                     /locus_tag="ACHUPB_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         9492 a        10056 c         9371 g         9673 t
ORIGIN      
        1 agggttatga cgaatatcac cttacagaaa caacatcgta cactgtggca ttttattccg
       61 gggttagccc tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc cattcccgcg
      121 gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct tgttggggat ggttttaggc
      181 aacaccatct atccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa
      241 tatttattac gtctgggtat cattctttat ggcttccgtc tgacgttctc gcaaattgcc
      301 gatgtcggta tcagtgggat catcattgac gtgttgacgc tgtccagtac cttcctgctg
      361 gcttgcttcc tggggcagaa agtgtttggt ctggataagc acaccagctg gttgatcggt
      421 gccggtagca gtatctgtgg tgctgccgcg gtactggcga ctgagtcggt agtgaaagcg
      481 gaagccagta aagtaaccgt ggctgttgca accgttgtta tcttcgggac cgtcgcgatt
      541 ttcctctacc cggcgatata tccgctgatg tctcaatggt ttagtccgga aactttcggt
      601 atctacattg gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc
      661 agcccggatg cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat gatgctggct
      721 ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag
      781 aaaagcaaaa tcactattcc gtggtttgcc atcttgttca tcgtcgttgc catctttaac
      841 tcgttccacc tgttaccgca gagcgtggtg aacatgctgg taacgctgga taccttcctg
      901 ctggcaatgg cgatggcggc gctgggtctg accactcacg tcagcgcgct gaaaaaagct
      961 ggggcgaaac ctctgctgat ggcactggta ctgtttgcct ggctgattgt tggtggtggt
     1021 gcgattaact atgtgattca aagcgtcatc gcataaacca ctacatcttg ctcctgttaa
     1081 cccgctatca ttaccgtttt cctccagcgg gtttaacagg agtcctcgca tgaaatacat
     1141 tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg ccgtcgaaat
     1201 cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct
     1261 cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa aaatatcact acacatcggc
     1321 gcaaattctt ccccacgaca gttatctgat taacctcgga catccggtca ctgaagctct
     1381 ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac tggggctttc
     1441 tttgctcaac ttccaccctg gcagccatct gatgcagatt tcagaagagg attgccttgc
     1501 gcgtattgcc gaatccatca acattgcgct ggataaaact caaggtgtaa cagcggtgat
     1561 tgaaaacacc gccggtcagg gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat
     1621 tatcgacggc gtggaagata aattccgcgt cggcgtctgc attgatacct gccatgcttt
     1681 cgctgccggg tatgatttgc gtactccagc cgaatgcgaa aaaacgttcg ctgattttgc
     1741 ccgtattgtc ggctttaagt atctgcgcgg gatgcacctt aacgatgcga aaagtacctt
     1801 tggcagccgc gttgaccgcc atcatagcct cggtgaaggc aatatcggtc atgatgcgtt
     1861 ccgctggatc atgcaagacg accgtttcga cggcattccg ctgatccttg aaaccatcaa
     1921 cccggatatc tgggcagaag agatcgcctg gctgaaagcg caacaaactg aaaaagcggt
     1981 ggcctgatcg atgaacgaca gggaaaagca gattcttaaa attttacggc gtaacccgct
     2041 gattcaacag aatgaaatcg ccgatattct gcaaatcagc cgctcacgtg ttgccgctca
     2101 tattatggat ttgatgcgca agggtttgat taaaggcaaa ggatatatcc ttaccgagca
     2161 ggattactgt gtggttgtgg gtgcaatcaa tatggatatt cgcggaatgg cggatatccg
     2221 ttacccgcaa gcagcctctc atccaggttc tgttcactgt tccgcaggcg gcgtcggacg
     2281 taatatcgcg cataacctgg ctctgcttgg tcgggatgtg catttaatat cagctattgg
     2341 caacgatttt tacggcgaga cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc
     2401 aaactgcatt cgtctgcatg gacacagcac tgcaacttat ctcgctatcg ctaataagca
     2461 ggaagaaact atcctggcaa tcaatgacac acatattctg caacagctaa cgcctcaact
     2521 gctcaatact tcacgggact tgattcgcca tgctggtgtg gtgctggctg attgtaatct
     2581 cacacctgaa gccctggagt gggtctttac cattgctgat gaaattccga tgtttgtcga
     2641 taccgtttcc gaatttaaag cgaacaaagt aaaaaactgg tacagccgga tccacactct
     2701 gaaaccaaca caaaatgaac tggaaatttt gtgggggcaa ccgattaaag acgacaacga
     2761 tcgcatcaga gctgtaaatt cgctgcatca acaaggtgtt aaacgcattt ttgtttatct
     2821 gaaagatgaa tctgtatttt gcagcgataa ggatggagaa caatttttgc tgaccgcgcc
     2881 agcgcatacg acagtagaca gttttggtgc tgacgatggt tttatggcgg gcctggtata
     2941 tagctttctt gaaggataca gtttccgcga cagcgcccgt tttgcggtag cctgcgcggc
     3001 aatttcgcgc gccagcggca gcttaaacaa ccctaccctg tctgccgata acgcgctttc
     3061 attagtgcca atggtgtaac aatgttgccg gattcgacgc ttgaccgcat cttatccggc
     3121 tcataaattg cgcactacgc taaaccaata aagaatcctg caatagtagc actcatcagg
     3181 ttagaaagtg tcgccgccgc cagcgcgcgt aaaccaagtt gggcgatttc cggcgcacgg
     3241 tgtggcgcaa ccgcagaaaa cgcccccacc accaccccga tagaaccaaa gttagcgaaa
     3301 ccgcacaacg cgaaagaaat aatcgccacg gttttagcat ccagagtgcc acccgtttgc
     3361 agatatggcg agaaattgag ataagcgaca aattcattga tcgccagctt ctgcccaatc
     3421 aaactcccgg caagatttgc atcactccag tcaaccccca tcacccacgc caatggggcc
     3481 aacaggtaac ctaaaatcga ctccagcgaa gcatgttcaa aaccaaacca gccgccaaca
     3541 ccgccgataa taccgttaat caacgcaatt attgcaacaa atgccataac aactgtcgct
     3601 acaccggcgg cgattttcag cccggtcatt gcccctgtcg cagcggcttc aataatgctt
     3661 tttggcggtg tttcggtgaa agagaggtta ttaaaagaaa cctgcgaaga ttccgtagcc
     3721 gggcttaaca ggcgggcaaa caagatcccg ccagggatcg ccattaacga tgccgccagc
     3781 aaatattcca caggtacgcc cagtgcggcg taaccaatca ttgtcgaacc agcaatcgag
     3841 gccatgccac tacaaatcgc tgtaaataat tcattgcgat tcagacgatc gataaagggt
     3901 ttgacgatcg ccggaatttc gttttgcccg aggaaaatgg tggtgaccgc gacgaatgac
     3961 tcgatcttgc tgatatttaa agctttctgg aagataccgc cgagaatgcg aattaagatc
     4021 cccatcacac cgatgtagta gagaatactc accagtgcag tgacgaagat aattgccggt
     4081 aataccctga aaccaaagat aaatcctgcg ccatcaaata aggtatccat tttcggtccg
     4141 accagagaac cgaagataaa tgcgctacct gcgtcgctgt acgccatcac tttgtggacg
     4201 ccaaaagcga ctttttctgc gacccaacgc cctggcggta accaaagcat aatcccgcca
     4261 atcacgacct gtaacactaa cgccgcgcca acggtacgca ggctgatctt cttcttgttt
     4321 actgacagta aaaacgcaat tgccagcagt accaccattc ccagaacact tctcatgaca
     4381 tccatagtga ttatctcttc atgtcatttt gggcgacggc tttctgccca tcagaaatag
     4441 ccgggcctgc atcttgcgat actgtcccgg cttgttatca acacttaatg ggtttttatg
     4501 tagccgcgta cgcactcttc gactaatccc cagaaccagt cggtatcaat agtgatgccg
     4561 actttagtat tggcgggctt gccaagaacg cccagctcgt cgcagacggt acgcccatag
     4621 caagggccac tgttgacgtc cacttcgacg tacatctcct gggttttaat gccatcaggg
     4681 ttaatcagat aaccgatgca ggtggcgtcg tgcaccgggc cgccagccag gccgtagttt
     4741 tcgaactgcg ttttgagagt gaagttcatg atgtcgctga acatctctcc ggcagggccg
     4801 cctgcccttt ccattcgtgc aattacgtcc ggggtgcaaa cggtctggtt ggtaagatcg
     4861 aggcccatca tcactaatgg aacgccggag gtgaacacca cgcgtgcggc ttccgggtcg
     4921 gcaaagatat tgaactcggc agatggggtg aagttgcctg taccgtaagc gccgcccatt
     4981 agcacaattt cacggatttt gggcaggatt gcgggttgca tacgcattgc caccgcgata
     5041 tttgaaagcg gaccaaccgg caccagagtg atatcgccat cgcttgccat aagggtatca
     5101 atgatgtatt tcaccgcgtg ggtactttct gcctggcggg tcagcggctc gaataccggg
     5161 ccatccagtc cggtttcacc gtgaatatta tcggcaacga tttgttgacg cataatgggt
     5221 tgcggcatcc ccgcataaac cggaacatta atctccagtt tctggcaaac attcaggcca
     5281 ttaattaatg ttttatcaag cgtctgatta cccgcaacaa tggtgatacc taataaatca
     5341 atcgccggat gtttcgccgc catcattata gcaatagcat catcatgacc cggatcacaa
     5401 tccagaataa tttttctctt ttccattgtt tatttcctct gtttccagtt gcgttatttt
     5461 ttctacagca acgaaatttc gatcgggccg atgattgaat cttaacaaca gcgtacgaat
     5521 gctaaatatg agaaatctca tagcggataa acatcgtgaa agaaatccac aataatgatc
     5581 ttaagcagca attgatgagt gaatccgcgt ttaaggattg ctttttaacg gatgtttcag
     5641 ccgatacgcg gctgtttcat tttttagcgc gtgactacat tgtgcaggaa gggcaacagc
     5701 cgtcctggct gttttacctg acgcgaggcc gcgccaggct ttacgccacg ctagctaatg
     5761 gtcgcgtgtc gctgctcgat ttctttgccg ccccctgttt tattggcgag attgagttaa
     5821 tcgacaaaga ccatgaaccg cgtgcggtgc aggctattga agagtgttgg tgccttgcgc
     5881 tccctatgaa acattaccgc ccgctgttat taaacgacac gctattttta cgaaaactct
     5941 gcgtcacctt aagtcataaa aattatcgta atattgtttc tttaactcag aatcaatcat
     6001 ttccgttagt gaatcgcctg gcagcattta tattactctc gcaggaaggt gatctttatc
     6061 acgaaaagca tacgcaagcg gcagagtatt taggcgtttc ttatcgacat cttttatatg
     6121 ttctcgcgca attcattcac gacggtttat taattaaaag caagaaaggg tatctaatta
     6181 aaaacagaaa gcagttgtca ggactggcgc tggagatgga cccggagaat aaattctccg
     6241 ggatgatgca gtaaaaatta ttggcaatag cacgattgcc ggatgcaacg cttaataagg
     6301 cgtacatccg gcaatgaaac tcaagctaaa ccaataaaga acccggcaat ggtcgcgctc
     6361 atcaggttgg aaagcgtcgc cgccgccagc gcccgtaaac caagctgggc gatttccggc
     6421 gcgcgttttg gcgaaatagc cgaaaacgct ccaacgacaa caccgataga accaaagttt
     6481 gcaaacccac agagggcgaa cgagataatg gcaatggtct tgacatccag cgtgccgctc
     6541 gtttgcagat agggcgagaa attgagataa gcaacaaact cattaatcgc cagtttctgc
     6601 ccaatcaaac ttccggcaag atttgcatca ctccagtcca cccccataat ccatgccaac
     6661 ggggccagca catagccgaa aatgctttcc agagagacat tggcaaaacc aaaccagccg
     6721 ccaactccac caataatgcc gttgatcagc gcaatgatcg ctacaaatgc cattaccacc
     6781 gtcgccacac cggcggcgat tttcagcccg gtcattgcac cgttcgccgc agcttcaata
     6841 atgctttttg gcggtgtttc gctaaacgac agattttcaa acgtaacttg cgaaggttcc
     6901 gtcgccggac taagaatacg tgcaaacagg ataccgccag gaatagccat cagcgaagcg
     6961 gctaacaaat agtcaatcgg cacccccatt ccggcatagc caatcatcat cgaacccgca
     7021 atagacgcca tgccgctaca aatagccgta aatagctcgt tgcgattcat gcgatcaata
     7081 aaaggtttaa cgatcgccgg gatctcattt tgcccgagga aaatagtggt gactgcgaca
     7141 aaagattcga ttttgctgat gttgagggct ttctgaaaaa tactgccaag aatacgaatc
     7201 agcagcccca ttacgccaat gtagtacagc aagctgataa gcgcggtaac gaaaataatc
     7261 gccgggagta cgcgaaaggc gaagataaag cccgcaccgt caaacagaac atccattttg
     7321 ggaccgacca gcgaaccaaa aataaaggca cttcctgcgt cactgtagga catcacttta
     7381 tggacgccta atgccgcctg ctctactgcc cattttcccg ggggaaaata gagcatgatg
     7441 ccaccaatgg caatttgcag cagcagcgca gcgccaaccg tgcgcaagct gatgctcttt
     7501 ttatttaccg acaacacaaa ggcaattgcc agcaatacca ccatccccgc aacacttctc
     7561 attatatcca taatgatttt cccttcatgc cagtaaaccc ggcgtcagcg ccaggttttg
     7621 gtatgtttga tgagtacggg cgacggcttt ctgcccgcca gaaattaacc cacgagacgc
     7681 tgatattctt tggcaatttc gctcgccaga atggcgttat taaagaccag ttgaatattg
     7741 gatttcaggc tatcaccgcc ggttagctcc gcaacacgcg ccagcaggaa cggtgtactc
     7801 tctttcccaa taacaccctg ttcttcggct tcagcaacgg cttgatcaat tgctgcattg
     7861 atggtttctt ctggcatcgc aaactgttcc gggatcgggt tcgctaccac caggccgccg
     7921 ttcaaaccgg tttgccactt cactgccatc gcgcgagcaa tttcgcgggc actgtcaaga
     7981 cgaatgctga cttcaaatgg gctggttcgg cagaaaaacg caggcagcgc ttttgtctga
     8041 taaccaatta acggtacacc gaaggtttct aaatattctg ttgttaatcc taaatcgaga
     8101 atagattttg cccccgcaca aacaacagtg acattggtat ttgccagttc ctgtaaatcg
     8161 gcagaaatat cgaaggtatg ttccgctccg cgatgcacac caccaatacc tccggttgca
     8221 aaaactttaa tcccagccag tgctgcaata atcatggttg atgcaacggt agtcgcgcca
     8281 tttttccctg cagcaacgac aaaaggtaaa tcacgacgac taactttcgt cacgttatgt
     8341 ccttcacggc ccagcaattc gatttcttct ttacttaagc ccactttcat cacaccgcca
     8401 ataatggcga tagtggctgg tactgcgccc tgtttacgaa ttgtttcttc tacttcaatt
     8461 gcggtttggg cattttgtgg gaatggcatt ccgtgagaaa ttatggtcga ttccagcgca
     8521 acaaccggtt ttttattttt taaagcatcc tgtacttcag cggatatttg taataattca
     8581 ggggacagag ttaattcaga cattctgtgt tctccactaa cgatctaaca ttggcaattg
     8641 ataattcagg gttgttggta tattcacagg caagtgccat tgacgaacat ccctgcgcaa
     8701 atcgaacaga atcgataaac ggcataccgt ctacccaaca ggaggccagc cccgccatca
     8761 tggcgtcgcc tgccccagta acattgatga cattcgtttt aataggggcc gaccacccac
     8821 tttcaccgtt gatatcactg tagtaaacgc catcccctcc catgctcagc accaagcggt
     8881 tcaggccgtg ttgatgaaac caggcggcga cctttgcgac atcttcacgt ccggaaagtg
     8941 caattccgct caacgtttcg gcttcaagac ggttaggttt taacgtatgg attttgctca
     9001 gatgatcgcg gattttgacg catttccatg ccgataccgg atcaataaat acaggcgttt
     9061 ccccgctatt ttccagaacc cagaccagca cctcttcact aaggttgcaa tccgcgacaa
     9121 taacctttgc accgcgaata aactcctgat gctgggcaag aaattctgca gaaatacagt
     9181 cactaatact catatcattt atcgcgacaa gcatttctcc tgtattatcg agcagtgata
     9241 aatagctgga tgtattctca cccggaacaa ttaagcattt atcgacataa acaccggatt
     9301 gattagtttg cgccagtaat gattgcccat aaaaatcacc acctactgca cttaaaagcc
     9361 aggcattttt ccccagtaac gccagattat gagcgatatt ccttcccacg ccacctgggg
     9421 taaatttaat ttttccggga ttcgaatccg cataattcaa tggatgatgt gaatacccag
     9481 ccacatcaat attcgccgaa ccaataatta ctatgtagtc cttttcgcgc atagcacgcc
     9541 ccctggcaat aaattgccaa cgaagaaatt gggtaaaata gccattacag tcaataagac
     9601 tattagagca cgtgttcaga tttaaacaca tgttcataat aagcatattt aacgtaatgt
     9661 aaatcgatca gaaaaaacga aatcggccag atatgaaggc ggtcacaaag tttgtctatt
     9721 tatgcagcaa caaaagagaa agttgtgatg tgtaacacac aatatggcag gcaagaaaaa
     9781 gggcagagat gtttctgccc tccagattgt tgacaacgcg cgctttgttc atcctggatg
     9841 tggcgtaaac gccttatccg acctacaaaa gcgcaaaaaa ttcaatatat tgcagggttc
     9901 atgtaggtct gataagcata gcgcatcagg caattttgcg tttgtcatca tcggggcaga
     9961 gatgtctcat ccccgataat gaagataaat tatgcgtttt tttctactat ttgtgtttcc
    10021 gggcgtttca ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc
    10081 aggtaaccca ataccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgt
    10141 ggtgccatca gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccaccaacg
    10201 atacagcacg gtagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa
    10261 ataaagcaca gaccaagtac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat
    10321 ttgcgacgcg ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc
    10381 ataatcgccg ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac
    10441 gctgctttgt ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc
    10501 agcagaaccg cattcgcagt ccccatggtc tgcagccagt gagtcaaccc atcgagaatg
    10561 ccagcaaccg gtttaccgat taggtagatc atcgccagac cgaccaccag actggaaatt
    10621 agcggaatga tcaggatcgg tttcagcgcc tccatactct gtggcagttt caattgcgta
    10681 ctgattaact tcgcaatgta accagccagg aagccagcaa ttataccgcc aataaagcca
    10741 gaaccggtgc tgaccgccag cataccgcca atcagacccg gtgtgaggcc cggacgatcg
    10801 gcaatggaga aggcaatata acctgccagt accggcacca tcagcgcaaa ggctgaacca
    10861 ccaccaatct gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca
    10921 aaagcaaaag aaagcgcgat acacagacca cctgcaacaa ccatcggcag catgtaagag
    10981 acgcccgtca gcaagtgacg gtaagcgcct gcactctctt tcttaccttc agtggtcgct
    11041 gtttgagctt tgcccgctgg ttcatacggc gttgcttcag caaccgcttt atccagttcc
    11101 tgcgcagttt tcttcagcgc cagaccggta gaggtgcgat acatcggttt accagcaaat
    11161 ttcgccagat ccacttcgat atctgccgcc acaatcacca gatccgctgc tgcgacttct
    11221 tcaggggtga ttgcattgcc cgcgccaaca gaaccacggg tttcaacttt cacccaccag
    11281 ccacgttttt tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca
    11341 gtcgggcaag cagtcaccgc aactacgcgt ttcggaccgc tggcagcaac cggtgctgtc
    11401 gcagcgaccg gcgcagtgta aggtttcgca tggcctttgg cttcactcag gaacagctca
    11461 gggtgcgcaa ctgcccggga aatatcaccc agccagacat ttttaccgtt cagcgcgctg
    11521 tcattcggga tggaatcacc gagaacaatc gccatttctg cgtcgttcgg attgtcgatg
    11581 atttccagtt ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt
    11641 gcctgaccga gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca
    11701 gttaaaaggt tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat
    11761 acccacattg ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg
    11821 ttcactggat tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc
    11881 gccaacggtg cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt
    11941 aacccaaagc gcgccttcgg cacccagtga aataacaaca tgcgcaatac cttgttcacg
    12001 tagcgcatgt gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat
    12061 ttccaactcg cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa
    12121 cgcttcacgg ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca
    12181 gtcggtgaac gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc
    12241 gaactgaccg agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgagggtgac
    12301 ttcaaaaccc gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt
    12361 aattcgggtg cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag
    12421 ttgctgaaaa ccatcctgat tgtctttacc caggaagccg ccaacggtga catcaattcc
    12481 caggtctttt aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt
    12541 tttcaccagg ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc
    12601 cggattaagg gtgatcgtag caacacgtct gctcattatg cgccctcccc aagaccagca
    12661 gcgatagcgt cgccaatggc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag
    12721 cgtaggcgat gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt
    12781 gccggtttgc cggtgccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg
    12841 gtattgacca gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac
    12901 tccgcgctta acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt
    12961 gccgcatccg ctttcagcaa gcggtcagct ttattgtcga gcaataaatc agcgagacgc
    13021 ttaagaaccg cgatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt
    13081 tcgccgtcca catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg
    13141 ccttcggcgc tatcgctcag ccagataccc tgtccgagat tcagcggttg ttcattgatg
    13201 gctttggtga cgaaagtggc gtcaactgcc cccacctctt tcagacgcgc agcgttcagc
    13261 gcctgaagag tcagcagatc gctggcgacg atatccagcg tcagcatttc gttgtcgagc
    13321 ttcagctgct cactctgctt ttcgcccatc agtaatgcgc gaagttcttc tgctgttgtt
    13381 gctgacttca gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc
    13441 aggcccagat gttcatccga gctggcagca ataccgattg ccacgtacgc tacctgaccg
    13501 tcaccccagg tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga
    13561 tcgcgggtgt cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt
    13621 tgctgttcgc gcgccagcat gccattgacg tagccttctg ctacattacc ggcctgcacc
    13681 agcgccgcag cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg
    13741 atgtcctgta cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag
    13801 cctctatgag aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac
    13861 gtttcaagaa agcataatac ttctgtttca gcacgcaagg aaagccgtaa agttatgagc
    13921 aaaagtttga tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc
    13981 gttatcagca atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt
    14041 ttgattattc gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa
    14101 ctccgcgtct tcctcttcca gtgatcgacc tgcatgcata actcccccgc agtctccagc
    14161 gcgaaatcgt ttgacctgac ctcgacggcg tttttaatcg ttgcctttct caccggtatt
    14221 gcgggtgctc tgcaaacccc gacactcagt attttcctta ccgatgaagt acatgcccgt
    14281 ccggcgatgg tgggattctt ctttaccggc agcgctgtca ttgggattct ggtaagtcag
    14341 tttctcgctg ggcgctctga taagcgcggc gatcgcaaat cgctgattgt cttttgctgc
    14401 ctgttaggcg tgctggcctg cacccttttt gcctggaatc gcaactactt tgttttgcta
    14461 ttcgttggcg tctttcttag cagctttggc tcgaccgcta acccgcaaat gtttgccctt
    14521 gcccgtgaac atgccgacaa aaccggacgt gaggcggtga tgttcagctc ttttttacgc
    14581 gctcaggttt cactggcatg ggtcattggc ccaccgctgg cttatgcgtt agcgatgggt
    14641 ttcagcttta cggtaatgta tctgagcgca gcggtagcgt ttattgtttg cggcgtgatg
    14701 gtgtggctgt ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg cacggttgaa
    14761 gcgccgcgcc gtaaccgtcg cgatacgcta ctgctgtttg tcatttgtac attgatgtgg
    14821 ggctcgaaca gcctgtacat catcaacatg ccgctattta ttatcaacga actgcatctt
    14881 cccgagaaac tggccgatgt gatgatgggg accgccgccg ggctggaaat cccgacgatg
    14941 ttgattgccg gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg cgttgctgcc
    15001 gtgggtggcg tctgttttta cgcaggaatg ctgatggcgc attcacctgt cattctgttg
    15061 ggcttgcagc tgctaaatgc tatttttatt ggcattctgg gcggcatcgg gatgctctat
    15121 tttcaggatc tgatgcccgg tcaggcgggt tcagccacca cgctctatac caacacttcg
    15181 cgcgtgggct ggatcatcgc aggatcagtg gcgggcatcg tcgccgaaat ctggaattat
    15241 cacgctgtgt tctggtttgc gatggtgatg attatcgcca ctctgttttg cttactgcgg
    15301 attaaagatg tttaaggcgc ggtcagcatt tccagatcga taagccaggt catggcctgc
    15361 tgacgcgagt taccgcacat ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc
    15421 agtggcgagg tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca gggcgtattg
    15481 gcgggcttgc catctggcat accgggaata gggctggaaa ttgaaggggc agtgcaacac
    15541 gccccgcaac ccggacggca ttccatacgt tgttctctta aggttaacca ccggattgat
    15601 gagcgcgcac agtaccacct tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa
    15661 ggcccgtcgc gagtactttg tcgcgatatt tttgacattt tcgactacag gaatttttcg
    15721 atgccaagag cgaacgaaat taaaaaaggt atggtactga attacaacgg caaactgctg
    15781 ctggttaagg atattgatat tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa
    15841 atgcgttttt ctgatgtccg taccgggctg aaagtagaag agcgtttcaa aggtgatgat
    15901 atcgttgaca ccgtgacgct gacccgccgt tacgttgact tctcctatgt cgatggcaac
    15961 gaatatgtct ttatggataa agaagactat accccgtata ccttcaccaa agatcagatt
    16021 gaagaagagt tgctgtttat gccagagggc ggcatgccgg acatgcaggt gctgacctgg
    16081 gacggtcaac tgctggcgct tgagctgccg cagaccgttg atctggaaat cgttgaaacc
    16141 gcaccaggca tcaaaggggc atctgccagc gcccgtaaca aaccggcgac attgagcact
    16201 ggtctggtga ttcaggtacc agaatactta agcccgggcg aaaaaattcg tatccatatc
    16261 gaagaacgcc gttatatggg gcgtgctgac taacttcagc cgcatgcaga aaagggatag
    16321 ctcaggctgt ccctttttta atttattata ccaatcttct attttgcgct tcctgaacac
    16381 cttacgccct caattttcac tcgttgattg atcgccctca cacttcatcg cattaacaat
    16441 ccagaccaat ttcaattgct gtcatataac tttacactgt cattgttaat ttatcgttac
    16501 taagacgtga ctcctatgaa gacaattgcc tccgttacgc tcccgcatca tgtacacgct
    16561 ccacgctacg atcgccagca gttgcaatca cgtatcgttc attttggctt tggcgccttt
    16621 caccgcgctc atcaggcgtt actgaccgat cgtgtgctga atgcccaggg cggcgactgg
    16681 gggatctgtg aaatcagctt gttcagcggt gatcaactga tgagccagct ccgcgcacag
    16741 aaccatttat ataccgtgct ggagaaaggt gcggacggca atcaggtgat aattgtcggt
    16801 gccgttcacg aatgccttaa tgcaaaactg gattccttag cggcaattat tgagaaattt
    16861 tgcgagccac aggtggcaat tgtttccctg acgattaccg aaaaaggcta ttgtattgac
    16921 ccggccaccg gtgcactcga caccagtaat ccgcggatta ttcacgatct acaaacccct
    16981 gaagaacctc actccgcacc gggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc
    17041 ggccttacac cgtttaccgt gctctcctgc gacaatattc ccgacaatgg ccatgtggtg
    17101 aaaaacgcgg tgctgggaat ggcagaaaaa tgttcgccag aactcgccgg gtggataaaa
    17161 aaacacgtca gttttccggg aaccatggtc gaccgcattg ttccggctgc aaccgacgaa
    17221 tcactggcag aaatcagcca gcatctgggg gtaaatgatc cctgcgcgat tagctgcgaa
    17281 ccgtttatcc agtgggtggt tgaagataac ttcatcgctg ggcgtcctgc ctgggaagtc
    17341 gcaggtgtac aaatggtgaa tgatgtcctg ccatgggaag agatgaaact gcgtatgctt
    17401 aatggcagcc actcttttct cgcttatctg ggttacctct caggattcgt ccatatcagt
    17461 gattgcatgc aggatcgcgt atttcgccat gctgccagaa cattaatgct ggatgagcaa
    17521 gcgccgacac tgcaaattaa agatgtcgat ttaacacaat atgcggataa gttaattgca
    17581 cgttttgcta atccgtcgct gaaacataag acctggcaaa tcgcgatgga tggcagccag
    17641 aaattaccgc aacgtatgct ggcaggtatt cgcatacatc tggggcgtga aacggactgg
    17701 tcgttgctgg cattaggcgt tgctggctgg atgcgttacg tcagcggcgt tgatgatgcc
    17761 ggaaatgcca ttgatgttcg cgatccgctt agcgataaaa ttcgcgaact tgttgcgggc
    17821 agcagcagtg aacaacgcgt aaccgccctg ctttccctgc gtgaagtttt cggtgatgat
    17881 ctgccagata acccgcattt tgtgcaggcc atcgaacaag cctggcaaca aatcgtacaa
    17941 ttcggcgcac atcaggcgct attaaacacc ctcaaaattt aacgatttct gcggttaaag
    18001 cggatgaagc tcaccttcgt ccgctctccc cttctctttt ctgccttttt tagccaggat
    18061 taacgctcag ttaacttacc agagtattgc ggagccgttg tgaccaggac caacctcatc
    18121 accggttttc tcggcagcgg gaaaaccacg tcgattcttc atctgttagc ccataaagat
    18181 cccaacgaaa aatgggcggt actggttaat gaatttgggg aagtcggaat tgatggtgct
    18241 ttgctcgccg atagcggcgc attgctgaaa gagatccccg gcggctgcat gtgctgcgtt
    18301 aatggtttac ccatgcaggt agggttgaat accttactgc gtcagggaaa accagaccgc
    18361 ttgttgatag agccgaccgg gctgggccat ccgaaacaga tcctcgatct gttaaccgca
    18421 ccagtctatg aactgtggat agatctgcgc gccaccttgt gcattctcga tccacgcctg
    18481 ctgctggacg aaaaaagcgc cagcaatgaa aacttccgtg accagctggc tgccgcagac
    18541 atcattgtcg ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc gctacagcgt
    18601 tggtggcagc aaaatggtgg cgatcgacaa ttaattcaca gtgagcatgg gaaagttgac
    18661 ggtcatcttc tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat
    18721 tctcatcagc atgtcgtgaa aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc
    18781 tggcgtcgca gtctgaacag cgggcaagga tatcaggcct gcggctggat attcgacgct
    18841 gatacggtat tcgacaccat tggcattctg gaatgggcgc gacttgcacc ggtggaacgc
    18901 gtcaaaggcg tgctgcgtat tcccgaaggg ctggtacgaa tcaaccgtca gggcgatgac
    18961 ctgcacattg aaacgcaaaa cgttgcgcca ccggacagcc gtattgagct gatttccagc
    19021 agcgaagctg actggaatgc tttgcagagc gcgctgttga agcttcgttt agcgactacc
    19081 gcgtaaggtt gcctgcgttt ttcagtaaga taattagaga aaatatgatt aaaaatttgc
    19141 cgcaaatagt gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg tatattcccg
    19201 ttaatcatgg attctggttg ccgattgatg cggatatttt ttatttcttt aatcagaaac
    19261 tggtcgaaag taaggccttt ttgtggctgg ttgcattgac caacaatcgc gctttcgacg
    19321 gttgttctct gctggcgatg ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc
    19381 ctggcagacg acgtatcgtg attatgggtc tggtcatgct attaactgca gtggtattaa
    19441 accagctggg tcaggcatta attcctgtaa aacgggccag cccaacattg acttttaccg
    19501 atattaaccg cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca cgagatagct
    19561 ttcccggcga tcacggcatg atgctgctta ttttttcggc attcatgtgg cgttatttcg
    19621 gcaaagttgc aggccttatc gcccttatta tttttgtggt ttttgcattt cccagagtaa
    19681 tgattggcgc acactggttt actgacatca ttgtcggttc gatgaccgtg atattgatcg
    19741 gtttgccctg ggtgttgctg acgccattaa gtgatcgatt aatcaccttt tttgacaaat
    19801 cactaccggg aaaaaacaaa catttccaaa acaaataact cacagtaatt aacatcatca
    19861 ggcttatttt tatagtgagg ataatcctga tgatgcgcac cgtgctttca tccatcgcgc
    19921 gcaaaaatca ttctctaagt aaatgaatgg attgcatgcg tttcactcaa tcgtacttta
    19981 attgaccaat cccgcttatt aactttctgt atcacttttt cttataaaaa atcatgtaaa
    20041 accgctcgcc aagaccgcac caatcgggta atctagaact cgttttgcct cggcggtaga
    20101 ttatcctcac agcatataat tttgtgcgtt agtccacaga tttggcctta aggaattgtt
    20161 tcaacatgcc caggtaatta gtctcgtgtc gcttggcatt tttttataac gatatttgtc
    20221 gttaaggact tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata
    20281 tcttgcgcgg gatccccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata
    20341 acaccgcaaa gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc
    20401 aagcttctca ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta
    20461 tggatcagta tgctgactgg aaaggcgtac gttatcgtct gggcggcagc actaaaaaag
    20521 gtatcgattg ttctggtttt gtacaacgta cattccgtga gcaatttggc ttagaacttc
    20581 cgcgttcgac ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta
    20641 cgggtgattt agttctgttc cgtgccggtt caacgggacg ccatgtcggt atttatatcg
    20701 gcaacaatca gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg
    20761 aaccgtactg gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa
    20821 ccgtttggat gcaatccctt ggctatcctg acgagttaac tgaaagcact gcttaggcag
    20881 tgcttttttg ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata
    20941 gtccggtcat tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt
    21001 tcatacgcgc tcccaattct ggacgtaagc tcctgcttac ctgcattgta gcaggcgtga
    21061 tgattgcgat actggttagc tgccttcagt ttttagtggc ctggcataag cacgaagtca
    21121 aatacgacac actgattatc gacgtacaaa agtatctcga tacctatttt gccgacctga
    21181 aatccactac tgaccggctt cagccgctga ccttagatac ctgccagcag gctaaccccg
    21241 aactgaccgc tcgcgcggcg tttagcatga atgtccgtac gtttgtgctg gtgaaagata
    21301 aaaaaacatt ctgttcatct gcgactggtg agatggacat tccactcaat gaattgattc
    21361 cggcgctcaa cattaataaa aacgtcgata tggcgatctt acccggcacg ccgatggtgc
    21421 cgaacaaacc cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat agcggtgtgt
    21481 ttgccgctct gaatctcaac ctgacgcctt ctctctttta tagttcacgg caggaagatt
    21541 acgatggcgt cgccctcatt attggcaata ctgcgctatc taccttttct tcacgtttga
    21601 tgaacgttaa tgaattaacc gacatgccgg tccgtgaaac taaaattgcg ggcattcctc
    21661 tgaccgttcg gctttatgcg gatgactgga catggaacga tgtgtggtac gcatttttac
    21721 tgggcggcat gagtggaact gtcgttggcc tgctctgcta ttacctgatg agcgtacgta
    21781 tgcgccccgg cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggcgtatc
    21841 aaccggtggt ggatacacaa gctttgcgag taacgggcct ggaagtactg ctacgctggc
    21901 ggcatcctgt cgcgggagaa attcccccgg atgccttcat taactttgcc gaatcgcaaa
    21961 agatgattgt gccgctgact cagcacctgt ttgagttaat tgcccgcgat gccgcagaat
    22021 tagaaaaagt gctgccagta ggcgtcaaat ttggtattaa cattgcgccg gaccatctgc
    22081 acagcgaaag ctttaaagca gatatccaga aactgctcac ttccctgccc gcacaccatt
    22141 tccagattgt gctggaaatt accgagcgcg atatgctgaa agagcaagaa gccacacaac
    22201 tcttcgcctg gctgcactcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc
    22261 acagcgcgct tatctatctt gagcgtttta cgctcgatta tctgaaaatt gaccgtggat
    22321 ttatcaacgc catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc
    22381 tggcgaaacg cctcaatatg ctgacggttg ctgaaggggt cgaaacgccg gaacaggcgc
    22441 gatggctaag cgaacgcggc gttaatttca tgcaaggcta ctggattagt cgccctttac
    22501 cgctggacga ttttgtccgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc
    22561 ttacgtccct tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag
    22621 gaaatccatt gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg
    22681 gtgtgcaggc gcaggctatc aaggaaagct atgcctttgc cgtgctgggc gaaccccggt
    22741 acgcgtttaa tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga
    22801 taacgttgtc agccctcggc accttcgata atttcaaccg ctatgcactg cgcggcaacc
    22861 cgggcgcacg caccgagcag ctgtacgaca cgctatttac gacttccgat gacgaaccag
    22921 gcagttatta cccgctgatt gctgaaagcg cacgctatgc tgacgattat tcctgggtgg
    22981 aggtcgctat taatccgcgc gcccgttttc atgatggttc gcccattact gcccgcgatg
    23041 tagagtttac ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca
    23101 aaggcaccac cgtcaaagcc attgcaccgt taaccgtgcg cattgagtta gctaaaccca
    23161 gcaaagaaga tatgctgagt ctgttttcgc tgccgatatt tccagaaaag tactggaagg
    23221 atcacaaact tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg taccgcgtta
    23281 cgtcctggaa aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa
    23341 acttaccggt aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg
    23401 atgataatgt cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg
    23461 acgctaaaaa ctgggccacg cgttataccg gtaaaaattt cgataaaaaa tacatcatca
    23521 aagatgagca aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac
    23581 gtccggtatt cagcgatcgc cgggtccggg aagcaatcac tctcgccttt gactttgaat
    23641 ggatgaacaa ggcgttgttt tacaacgcct ggagtcgcac gaacagttat tttcagaata
    23701 ccgaatacgc ggccagaaat taccccgacg ccgcggagct ggtgcttctg gcaccaatga
    23761 aaaaagatct accgccagaa gtcttcacac aaatctacca gccgccggta tctaaaggcg
    23821 atggctacga tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa acgggctggg
    23881 tgctgaaggg tcagcaacgc gttaatgcca caacgggtca gccgctcagc tttgaattat
    23941 tgcttcccgc aagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc
    24001 tgggtatcaa tatggacatt cgcaaggtgg ataactcgca aatcactaac cgcatgcgca
    24061 gtcgcgacta tgacatgatg ccgcgcctat ggcgggcgat gccgtggccc agttccgatt
    24121 tacagatttc ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa
    24181 gcccggttat cgactcgctg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat
    24241 tactgccgtt ggggcgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa
    24301 tgtggtacat ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtac
    24361 gccctgttta cagcctgggt atcgatacct ggtggtatga cgttaataaa gcggccaaac
    24421 tgccgtcagc cagacaacag ggagagtaga tgggcgctta cctgattcgc cgtctgttgc
    24481 tggtgatccc aacgttatgg gctattatca ctatcaactt tttcatcgtg caaattgcgc
    24541 ctggcggtcc ggtcgatcag gccatcgccg ccattgagtt tggtaatgcc ggagtattac
    24601 ccggcgcagg cggtgaaggt gttcgtgcca gccatgcgca aacgggtgtc ggcaatatca
    24661 gcgacagtaa ttaccgtggc ggacgcggat tagacccaga agtgatcgct gagatcactc
    24721 atcgctacgg tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca
    24781 tccgctttga ttttggtgat agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag
    24841 acagtctgcc ggtttccatc accctcggat tgtggagcac gctgattatc tatctggtgt
    24901 cgattccgtt aggcattcgc aaagctgttt ataatgggag tcgctttgac gtctggagta
    24961 gcgcatttat catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg
    25021 tcttcttcgc gggcggcagc tatttcgacc tgttccctct acgcggcctg gtttccgcta
    25081 actttgattc gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctac
    25141 cggtgctagc gacagtgatt ggtggctttg cggcgctgac catgctgacc aaaaactcat
    25201 tccttgatga agtgcgcaag caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaga
    25261 aaaacattct ctggaaacat gtgttccgca acgccatgct gctggtgatt gccggttttc
    25321 cggcgacgtt tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac
    25381 tcaatggtct tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt
    25441 ttggtacctt gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca
    25501 gctatacgct ggttgatccg cgtatagatt ttgagggacg ttaatgtcgc gactcagccc
    25561 cgtcaatcag gcccgttggg cgcgttttcg tcataaccgt cgcggctact ggtcgttatg
    25621 gattttcctc gttttgtttg gtttgagttt gtgttctgaa cttatcgcta acgataaacc
    25681 gttgctggtg cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag
    25741 cgattttggc ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg
    25801 gctggaaaat aacggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa
    25861 ctttgctacc gataagccct tcccttctcc accctcccgg caaaactggc tgggaacgga
    25921 tgccaacggc ggcgatgtgc tggcacgcat tctctatggc acgcggatct cggttctgtt
    25981 tggcctgatg ctgactctct gttccagcgt gatgggcgtg ctggcggggg cgctacaagg
    26041 ctattacggc ggtaaagtcg atctctgggg acaacgcttt attgaagtat ggtcggggat
    26101 gccgacgctg tttttgatta ttttactttc cagcgtcgta cagcctaact tctggtggct
    26161 gctggcaatt actgtcttgt ttggctggat gagtctggtc ggcgtggtgc gggcggagtt
    26221 tttacgtact cgtaatttcg actacattcg tgcggcacag gcgcttggcg tcagcgatcg
    26281 cagtatcatc ctgcgtcata tgttgcctaa tgccatggtc gcgaccctca cctttttacc
    26341 gtttatttta tgtagttcga taaccaccct gacctcgctc gatttcctcg gcttcggtct
    26401 accgctcggt tcaccgtcac tcggtgaact gctgttacaa gggaaaaata accttcaggc
    26461 cccgtggctt gggatcaccg ccttcttgtc ggtggcgata ttattgtctt tgctgatctt
    26521 tattggtgaa gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa
    26581 ctctgttagc gattgaaaat ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag
    26641 tagtcaatga tgtttcacta cagattgagg ctggcgaaac gctggcgctg gtgggtgagt
    26701 caggttcagg caaaagcgtt accgcgctgt caattttacg cctgctccct tccccgccgg
    26761 ttgaatatct ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgatc
    26821 aaacgttgcg cggtgtacgc ggtaataaga tcgccatgat ttttcaggaa ccgatggtgt
    26881 cgttaaatcc attgcatacc ctggaaaaac agctttatga agtgctttca ctccaccgcg
    26941 ggatgcgtcg ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc
    27001 gccaggcggc aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc
    27061 gggtgatgat tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccga
    27121 ccaccgcact ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag
    27181 gcgagctgaa tatgggcatg ctgtttatta ctcataacct cagcattgtc agaaaactgg
    27241 cccaccgcgt ggcggtaatg caaaacggtc gctgtgtcga gcaaaataac gccgctacgc
    27301 tatttgcctc acccactcat ccttacacac aaaagctact caacagtgaa ccgtcaggcg
    27361 atccggtacc gttgccagaa cctgcctcaa cgttgctgga tgttgaacag cttcaggttg
    27421 ccttccccat tcgcaaaggg attttgaagc gcattgtgga tcataatgtg gtggtgaaaa
    27481 acatcagttt tacgctacgg gcgggtgaaa cactgggttt agtgggcgag tctggttccg
    27541 ggaaaagcac gacgggactg gcgctgctgc gactgattac ctctcagggc agcatcgtct
    27601 ttgacggtca gccactgcaa gatttaaatc gccgccagct gttacctact cgtcatcgta
    27661 ttcaggtggt atttcaggat ccaaactcct cgctcaaccc acgactcaac gttttgcaga
    27721 ttattgagga aggcttacgg gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac
    27781 aagtgatagc cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg
    27841 cggagttctc tggtggtcag cgacaacgta ttgcgattgc cagggcatta attcttaagc
    27901 cctcactgat catacttgat gaaccgacat catcactcga caaaacggta caggcgcaaa
    27961 tattgacgct attgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc
    28021 acgatttgca cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag
    28081 tagtggaaca aggaccgtgc gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc
    28141 agctactggc gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt
    28201 gccacaccaa aatttttcaa tcggcatgtt gcagcaaact cgtcctggcg atttacaaac
    28261 aggcacggct caccttcaca ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc
    28321 tggcttagtt tatacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc
    28381 gccaccaggc tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac cttcgaacct
    28441 gcgaaaccag cagaccagcg aaagttctgc ggtagacgat ggacgattga gagttgtaat
    28501 gaagtcactt attttccccg gaagcacatt tacttcacaa tttatttaca tcaattttaa
    28561 cacatcatca acaatccgtc tttatacaga tcgtaaagat tgatgcctcg cctgcatggc
    28621 ctctatggct ctgatttaag taatatctgc gggcgtggtc acgtttgcgg ggctatatgt
    28681 acccgtttct gcgatctaac tcaacctttt taactacaat gatgtgactt tttacataaa
    28741 ttgattttac ataaaataaa catatatcgg gggaatgata gatttgtggt tgacatgcat
    28801 caacaaagga agccttttag cttcctcgtt gtgcaataga tcaccgtttt ttcggtcgac
    28861 tggcgtacag acagaagaga atggagctgg ttgcgcagaa tgcaattgac caaatcatcg
    28921 gccatgcaga gttaaaggtc gcaagagaaa gcaatgcgcc aacaattgcc cctatgccaa
    28981 aacggaaggt tcctgccagc gaagatgccg ttcccgccat atggggaaac tcatcaagaa
    29041 tgaccgccat cgcattggat gacaccatag acacgcagcc cacaaacgcc gcaacgccaa
    29101 ccaccagcga ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    29161 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cggacgaagc
    29221 ggctgttaaa gatggtcatc acgaacagaa aaacaatgtt tagcgcaaag taataaccaa
    29281 agttttccgg tgcaacgtgg ttgatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    29341 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    29401 ggaacagcgc cgcaaagtta ccaatagtgg tacgaatgtg aaaaggctgg cgaggatcca
    29461 gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag
    29521 cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg
    29581 gtaaatatca cgcatcaaag cgttgataac cacgctggcc gcagccgcag ccagcccgtg
    29641 gaagaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc
    29701 ggcggcaaac accagcgtac cgccaagaac caccggctta cgcccgaagc tgtctgccat
    29761 cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactca gggtcatctg
    29821 cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat
    29881 atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga
    29941 cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct
    30001 ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt
    30061 aatgccgcca atacgttcac gatgcagctc aaccacatgg ttacccacgg cggcgaacat
    30121 gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat
    30181 cacttccagc accgccggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc
    30241 aaattgttct gccgtatcgt cagctacggg tgataccagc gtcaccagat aggtcttctc
    30301 gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatctg tcattagtac
    30361 cagaccggtg gtatcgatat ccagccgccc tgcggcgtgc agtttccacg ctaccggttc
    30421 atctagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa cataaccctg
    30481 aggcttattg agcatgaagt aacgtggacc gtgttgctgc gccagcgggt tgccatcgta
    30541 agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cggacgattt cgccatcgac
    30601 ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg
    30661 tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaaacg gaggtcgggt
    30721 aatgacccga atatctgaac tgttgtctgc ccagtatagc ggtctaataa catccctcaa
    30781 gggaaaaaga tccgtggcat actattagca gaataatcta cctacgcgag accatgattt
    30841 ttacacttcg cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc
    30901 ataaaacccc tgccgttatc gtgctgccca ccggtgcagg taaaagcctg gtgatagcgg
    30961 aactggcacg gctggctcgt ggtcgcgtac tggtgctggc acacgttaaa gaactggtgg
    31021 cgcaaaacca tgcaaagtat caggcgctgg ggctggaagc cgatattttt gccgccgggc
    31081 tgaagcgtaa agagagccac ggtaaagtgg tatttggcag tgtgcagtcg gtcgcccgta
    31141 atattgatgc ctttcagggt gaattttcgc tgttgattgt cgatgaatgt caccgtattg
    31201 gtgacgatga agagagccag tatcagcaaa tcctcactca cctgacaaaa gtgaatcccc
    31261 acttacgcct gctggggctg actgccacgc cttttcgatt gggcaaaggt tggatctacc
    31321 agttccatta tcacggcatg gtacgcagcg atgagaaagc ccttttccgt gactgcattt
    31381 atgagctgcc cctgcgttat atgattaaac acagctatct gacgccgcca gaacgactgg
    31441 atatgccagt agtgcaatat gatttcagcc gcttgcaggc acagagcaac gggctgttca
    31501 gcgaagccga tctcaaccgt gagctgaaaa aacagcaacg tattaccccg cacatcatca
    31561 gccagattat ggagttcgcc gaaaagcgta aaggagtgat gattttcgcc gcgacggtcg
    31621 aacacgcaaa agagattgtg ggattactgc ctgccgaaga tgcagcactg attactggcg
    31681 acacccccgg cgctgagcgc gatgtgttaa ttgaaaattt taaagcccag cgttttcgct
    31741 atctggtcaa cgtcgcggta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg
    31801 ccattctgcg ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc
    31861 gtctcgctcc gggcaagact gattgtttaa ttcttgatta tgcgggtaat cctcacgatc
    31921 tctacgcgcc ggaagttggt acaccgaaag gcaaaagtga caacgttccg gtacaggttt
    31981 tctgccctgc ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat
    32041 tgattgaaca ctttggtcgc cgctgtcagg gatggtttga agatgacgac ggtcatcgcg
    32101 aacagtgtga cttccgtttc cgttttaaaa attgcccgca atgtaacgca gaaaatgata
    32161 ttgccgctcg ccgctgccgc gaatgtgaca cggtactggt tgatccggat gatatgttaa
    32221 aagcggcgct acgactgaaa gatgcgctgg tattacgctg tagcggcatg tctttgcagc
    32281 atgggcacga cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggtgcgg
    32341 atgtgagtga gcgtttccgt ctgcaaacac ctgcccagcg taccgccttc gagcagcttt
    32401 ttatccgccc gcatacgcgc acaccgggca tcccgctgcg ctggatcacc gccgccgata
    32461 tcctcgccca gcaagcctta ttgcgacccc cggattttgt cgtcgcccgc atgaaaggcc
    32521 agtactggca ggtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg
    32581 aattacgcgg ttaatggtag ttttgattga tgtataaggc gattgagtat agaatctcac
    32641 ccgcttttgc atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt
    32701 aatttaagag agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag
    32761 ggtgcgagcc gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa
    32821 gaagcgccgc tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct
    32881 gaattctaca gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct
    32941 caggacgtac agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct
    33001 taattgctga ataagttgta aaaaaccccg ctcccgcggg gttttttgta tctgcagatt
    33061 atgcctgatt acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg
    33121 gcattcagaa cacaaaaccg attaattgcc gccagacgtc cggcgctgca attggtcgcg
    33181 caaattcggc ggcgtgcctt taatggtcag ggtatcggtc gccggctccc agaagatgcg
    33241 ttcacctaac agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt
    33301 cgtcaactga cgcagtgtgc tccggtctgc cgggaagctc tcttccagtt cgtagccttt
    33361 ttcagccgca aactccgtga agctcacttc gcttacgcct gccagctctt tcgacagcga
    33421 ttccagttcg atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg
    33481 gcgcacgttc tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc
    33541 ttgcaacagc ccacggtttt gtgctttcgt gttcaatcct tcgctggccc cgaggaaatc
    33601 cataaagaaa tccgccactt tgcgccctac tcgccctttc aggaaagtga gatagcgggt
    33661 ggactctgga ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt
    33721 gatatcaaga taatgggttg ggttgatgtc gagattttcg ttaacacgca tactgctcag
    33781 gttgctcaga actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag
    33841 cacgaatcca ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gaccagttgc
    33901 cgcgcggcta aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc
    33961 cagttcactc tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg
    34021 cagttctgcc accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac
    34081 cagctcaagg ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc
    34141 cagactcatg ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg
    34201 acgcaataaa agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca
    34261 taaaaaagca gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg
    34321 atttcgattt atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc
    34381 agagctgctc aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt
    34441 aggcaacatg gtcactaacc ttatcaacac cagcattgcc ccggcccaac gccaggcaat
    34501 tgccaactct tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg
    34561 gaaacagata acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga
    34621 tggtcagttg ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca
    34681 gccgttacct gtttatcgcc gactggccga caacgcttgc tggtcgcatt tattcctacg
    34741 taagcattat cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc
    34801 cgctgacctt tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa
    34861 cggcgggaat gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc
    34921 ttaatcccat cgtctggcag ttggttatca acccagacga aaatgagatg gcgcgcgact
    34981 ggcagctgat gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt
    35041 ggagctggca aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc ccgctggccg
    35101 cattcttatt tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact
    35161 tctatcgccc gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc
    35221 gacgttttct tgagaagcat ggtctgcttg atgcgcagga gtatcaacgc cgtcttattg
    35281 agcaaggtaa tccagacgcc gtttccgttc agtatccgtt aagcgaactg cgctatcgcg
    35341 atatgggcac cgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct
    35401 tcgagaagca gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc
    35461 atatgagctc cggcaacact acagacaacg gcatctttgg cctgttctat ggtatctcgc
    35521 cgagctatat ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt actgcgctta
    35581 atcagcaagg ctatcagctg gggttattct catcagatgg ctttaccagc ccgctgtatc
    35641 gccaggcatt gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga
    35701 ccgccacgca gtggatcaac tggctgggcc actacgcaca agaagataac cgctggttct
    35761 cgtgggtttc tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga
    35821 aatatagccg ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc
    35881 gtgattctgg caaactggac aatacggttg tgattatcac tgccggtcgg ggtattccgc
    35941 tgagcgaaga cgaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga
    36001 ttcactggcc aggcacgccg gcgcagcgta ttaatgcgct gactgatcat accgatctga
    36061 tgacgacgct gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag
    36121 gtcaggattt gttcaaccct caacgccgtc attactgggt tactgcagcg gataacgata
    36181 cgctggcaat taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca
    36241 cttacaactt acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc
    36301 aagtgctgac agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcga
    36361 aatatcttac ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt
    36421 agcgcagcct ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg
    36481 tgccgaccaa aaatcccaag aaaaaaccaa cctttacggt tggttttttt atatctgcaa
    36541 ttaatttgat aaacagaccg tgacacatca cagcctgttt attttctgtt atcagaacgt
    36601 ccagaccact cccgcctgag tattccacgg cgattctacc cctgcgccat tgccatagct
    36661 gactgacaaa tgaccgctta acgtcggggt aaacgatgac cttatcccag cctgataaac
    36721 gccacgggtt ccagccacat cattattaaa tttgccatcg tcattaactt tcacatggtt
    36781 agaatcggcg tattcctgac gcacggcggc tttcagccag ggttccaacg tcgtaccgtt
    36841 ttgcaggtcc atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt
    36901 tcccacatcc gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc
    36961 ggtaaaggcg agatagggtc taacactcca caatccgtca acccaacgga acccgctctc
    37021 aacatgagca cccgcgccgt tactattgta atcgccaaac gctgttgccc cattactcat
    37081 cttgccatgg atggtgttgg caaaacggtc aactttcacc accccatcaa cataggcacc
    37141 gttctgatgc tcccaaccgg cataagcccc cagggtgtag ctatcaatat tgcctttgcc
    37201 gccgcgatca aaaccaatat cagaatgaga gtaatcaaag aacaagccgc gaattgtgct
    37261 gctttcttca cgggagaaac ggctatcgat accgagcgtc aggcccgtca atgtttgctc
    37321 aaagccagct cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg aactccacat
    37381 cgccgtatcg taattaacgc cttttacgct accaagacgc tcacgcacgg tgtccagttc
    37441 tgcatcaaat accagcggtt gtgctgctgc catgttcagc acatcagtgg ttgaaggggt
    37501 aatttgcgcg cgattctctg cgaggctcca gctatggttg ccattatcca gcaaggtata
    37561 ttcatacgta ccgatatcaa caacgcctcc ggcattgccc aacgtgaatg cagcatcacc
    37621 gccgcccgtc gttaccagtg taaggctatc tcctgctgcc gggctggccc ccgtgtccgt
    37681 cacgaatatt ttgaaatcac ctgttgcctg accgatgacg ttgagctgat cgctctgatg
    37741 attagccata tcggtacgca tataaaattg ccgtttcccg acagggtatt ggtggtcaac
    37801 gtaatgaaat tttccgcact tgccgtcaca gatgaacgag tcactggctc agccatcata
    37861 atcacatttg cgccggacat cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc
    37921 gtagtgatat cggcaatgat ggtccatgcg ctattttcga gcgaaacgtc cgcttggctg
    37981 gtatccacgg caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc
    38041 aacatagcgg gtgcataatt tattttcggc ttcatgacct caagaatgcc atcattccct
    38101 ctgactgaca cgttaaccat tgtgccagcc cagacattag cgcggccatt gttgataacc
    38161 atattttcag agatggcttt agaggaatac gtgatacttc cattctgatc tgatcgaccg
    38221 agatcatata cagcatttgc ctgcactttc gtaccagtat cctttcccag cgattgcata
    38281 gtggcatgct catcaaggat agtgtcaatg gcctgagtgt cctccataac aatgagcccg
    38341 gagccatcat ccagttcata attttttgac acaccatcct ttatactaaa ttggccttta
    38401 ctgtttgtac cactcacttc cagcgcattc gttgagacaa ttaatttccc gcctgctttt
    38461 ttatcaacgc cagttgcagt cccgccatcc ataacctcca gtaatcctcc cttttcgata
    38521 gcggcaaaga cggcaatccc atctttttca acaattaagc gcccgccttt aatcgtggga
    38581 tcattcgccg cg
//
LOCUS       DAZOZD010000011        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOZD010000011 DAZOZD010000000
VERSION     DAZOZD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.011"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUPB_000450"
     CDS             59..1423
                     /locus_tag="ACHUPB_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0757362.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUPB_000451"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUPB_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0757363.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUPB_000452"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUPB_000452"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0757364.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUPB_000453"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUPB_000453"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0757365.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUPB_000454"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUPB_000454"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0757366.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUPB_000455"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUPB_000455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0757367.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUPB_000456"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUPB_000456"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0757368.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUPB_000457"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUPB_000457"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0757369.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUPB_000458"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUPB_000458"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0757370.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUPB_000459"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUPB_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0757371.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUPB_000460"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUPB_000460"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0757372.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUPB_000461"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUPB_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0757373.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUPB_000462"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUPB_000462"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0757374.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUPB_000463"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUPB_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0757375.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUPB_000464"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUPB_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0757376.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUPB_000465"
     CDS             13749..14288
                     /locus_tag="ACHUPB_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0757377.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUPB_000466"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUPB_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0757378.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUPB_000467"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUPB_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0757379.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUPB_000468"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUPB_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0757380.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUPB_000469"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUPB_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0757381.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUPB_000470"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUPB_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0757382.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUPB_000471"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUPB_000471"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0757383.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUPB_000472"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUPB_000472"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0757384.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUPB_000473"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUPB_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR0757385.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUPB_000474"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUPB_000474"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0757386.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUPB_000475"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUPB_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0757387.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUPB_000476"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUPB_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0757388.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUPB_000477"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUPB_000477"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0757389.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUPB_000478"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUPB_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0757390.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUPB_000479"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUPB_000479"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0757391.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUPB_000480"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUPB_000480"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0757392.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUPB_000481"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUPB_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0757393.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUPB_000482"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUPB_000482"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0757394.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUPB_000483"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUPB_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0757395.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHUPB_000484"
     CDS             complement(37465..37920)
                     /locus_tag="ACHUPB_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757396.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHUPB_000485"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHUPB_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZOZD010000012        37768 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOZD010000012 DAZOZD010000000
VERSION     DAZOZD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.012"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUPB_000486"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUPB_000486"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0757397.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUPB_000487"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUPB_000487"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0757398.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUPB_000488"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUPB_000488"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0757399.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUPB_000489"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUPB_000489"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0757400.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUPB_000490"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUPB_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0757401.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUPB_000491"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUPB_000491"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0757402.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUPB_000492"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUPB_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0757403.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUPB_000493"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUPB_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0757404.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUPB_000494"
     CDS             6537..6773
                     /locus_tag="ACHUPB_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0757405.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUPB_000495"
     CDS             6776..7633
                     /locus_tag="ACHUPB_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0757406.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUPB_000496"
     CDS             7654..9645
                     /locus_tag="ACHUPB_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0757407.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUPB_000497"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUPB_000497"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0757408.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUPB_000498"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUPB_000498"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0757409.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUPB_000499"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUPB_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0757410.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUPB_000500"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUPB_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0757411.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUPB_000501"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUPB_000501"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0757412.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUPB_000502"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUPB_000502"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0757413.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUPB_000503"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUPB_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0757414.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUPB_000504"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUPB_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUPB_000505"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUPB_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0757415.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUPB_000506"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUPB_000506"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0757416.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUPB_000507"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUPB_000507"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0757417.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHUPB_000508"
     CDS             complement(21570..22739)
                     /locus_tag="ACHUPB_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0757418.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUPB_000509"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUPB_000509"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0757419.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHUPB_000510"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHUPB_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0757420.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUPB_000511"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUPB_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0757421.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHUPB_000512"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHUPB_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0757422.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUPB_000513"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUPB_000513"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0757423.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUPB_000514"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUPB_000514"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0757424.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUPB_000515"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUPB_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0757425.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHUPB_000516"
     CDS             complement(30320..31576)
                     /locus_tag="ACHUPB_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0757426.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHUPB_000517"
     tRNA            31884..31960
                     /locus_tag="ACHUPB_000517"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHUPB_000518"
     tRNA            31965..32041
                     /locus_tag="ACHUPB_000518"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHUPB_000519"
     CDS             32149..32454
                     /locus_tag="ACHUPB_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0757427.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHUPB_000520"
     CDS             32580..34184
                     /locus_tag="ACHUPB_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR0757428.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUPB_000521"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUPB_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0757429.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUPB_000522"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUPB_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0757430.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHUPB_000523"
     CDS             complement(35794..36462)
                     /locus_tag="ACHUPB_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0757431.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHUPB_000524"
     CDS             complement(36526..37164)
                     /locus_tag="ACHUPB_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0757432.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37768)
                     /locus_tag="ACHUPB_000525"
     CDS             complement(37177..>37768)
                     /locus_tag="ACHUPB_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HFR0757433.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9023 a         9930 c         9380 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaacca cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccg
//
LOCUS       DAZOZD010000013        37457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOZD010000013 DAZOZD010000000
VERSION     DAZOZD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.013"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUPB_000526"
     CDS             complement(<1..716)
                     /locus_tag="ACHUPB_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0757434.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUPB_000527"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUPB_000527"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0757435.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUPB_000528"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUPB_000528"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0757436.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUPB_000529"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUPB_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0757437.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUPB_000530"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUPB_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0757438.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUPB_000531"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUPB_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0757439.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="ACHUPB_000532"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="ACHUPB_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="ACHUPB_000533"
     CDS             complement(7194..8075)
                     /locus_tag="ACHUPB_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0757440.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="ACHUPB_000534"
     CDS             complement(8096..9406)
                     /locus_tag="ACHUPB_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0757441.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIVLPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="ACHUPB_000535"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="ACHUPB_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHUPB_000536"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHUPB_000536"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0757442.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHUPB_000537"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHUPB_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0757443.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHUPB_000538"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHUPB_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0757444.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHUPB_000539"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHUPB_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0757445.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHUPB_000540"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHUPB_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0757446.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="ACHUPB_000541"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="ACHUPB_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0757447.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHUPB_000542"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHUPB_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHUPB_000543"
     CDS             complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHUPB_000543"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0757448.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHUPB_000544"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHUPB_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHUPB_000545"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHUPB_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0757449.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHUPB_000546"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHUPB_000546"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0757450.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /gene="puuA"
                     /locus_tag="ACHUPB_000547"
     CDS             20134..21495
                     /gene="puuA"
                     /locus_tag="ACHUPB_000547"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0757451.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="ACHUPB_000548"
     CDS             21644..21808
                     /locus_tag="ACHUPB_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757452.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="ACHUPB_000549"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="ACHUPB_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0757453.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="ACHUPB_000550"
     CDS             23317..23562
                     /locus_tag="ACHUPB_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0757454.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="ACHUPB_000551"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="ACHUPB_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0757455.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="ACHUPB_000552"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="ACHUPB_000552"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0757456.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="ACHUPB_000553"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="ACHUPB_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0757457.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="ACHUPB_000554"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="ACHUPB_000554"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0757458.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="ACHUPB_000555"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="ACHUPB_000555"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0757459.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="ACHUPB_000556"
     CDS             29225..29578
                     /locus_tag="ACHUPB_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0757460.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="ACHUPB_000557"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="ACHUPB_000557"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0757461.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="ACHUPB_000558"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="ACHUPB_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="ACHUPB_000559"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="ACHUPB_000559"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0757462.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34085..34188)
                     /locus_tag="ACHUPB_000560"
                     /pseudo
     CDS             complement(34085..34188)
                     /locus_tag="ACHUPB_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHUPB_000561"
                     /pseudo
     CDS             34242..36232
                     /gene="pdeR"
                     /locus_tag="ACHUPB_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHUPB_000562"
     CDS             complement(36214..36342)
                     /gene="ymiD"
                     /locus_tag="ACHUPB_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0757463.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHUPB_000563"
     CDS             36379..36552
                     /gene="yciZ"
                     /locus_tag="ACHUPB_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0757464.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36642..37391
                     /locus_tag="ACHUPB_000564"
     CDS             36642..37391
                     /locus_tag="ACHUPB_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0757465.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         8688 a         9911 c         9582 g         9276 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc acaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccag
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcgtccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgccgat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat
    34141 atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    34201 atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc gttagggagt
    34261 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    34321 gcgatgtttt gcgctttttt accaccgaaa ccacagaacc tgatcgtact ttgcagttat
    34381 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    34441 gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    34501 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcagttgct cgtgatttat
    34561 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    34621 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac actgggttga
    34681 aagaacacga cgtcattggc caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    34741 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    34801 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa ataaatttgt
    34861 ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg acattaccga
    34921 agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact
    34981 gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg ctgataacaa
    35041 taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg acgcctatgg
    35101 gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga
    35161 acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg catccaatac
    35221 ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac gcctcccctt
    35281 tcgtattggt ttaattgaag tttataccag ctgttcagta ggtatttcgc tctctcccga
    35341 acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt acacagcgaa
    35401 ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc gggtatttga
    35461 atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca
    35521 ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg
    35581 gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc
    35641 agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa
    35701 gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc
    35761 cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg
    35821 ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc
    35881 tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg
    35941 ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt
    36001 ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc
    36061 tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga
    36121 tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat
    36181 gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga
    36241 atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc
    36301 cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt ttccactttt
    36361 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    36421 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    36481 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    36541 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    36601 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    36661 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    36721 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    36781 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    36841 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    36901 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    36961 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    37021 acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    37081 ttggcccgtt gacacgccag tgcatccaac aggtgcattt cagcaaagca tttattggta
    37141 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    37201 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    37261 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    37321 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    37381 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    37441 taagatgatt cctggtt
//
LOCUS       DAZOZD010000014        37257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOZD010000014 DAZOZD010000000
VERSION     DAZOZD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.014"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..642)
                     /locus_tag="ACHUPB_000565"
     CDS             complement(<1..642)
                     /locus_tag="ACHUPB_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0757466.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="ACHUPB_000566"
     CDS             complement(742..1653)
                     /gene="gsiD"
                     /locus_tag="ACHUPB_000566"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0757467.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="ACHUPB_000567"
     CDS             complement(1656..2576)
                     /gene="gsiC"
                     /locus_tag="ACHUPB_000567"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0757468.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="ACHUPB_000568"
     CDS             complement(2594..4132)
                     /gene="gsiB"
                     /locus_tag="ACHUPB_000568"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0757469.1"
                     /translation="MAKAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="ACHUPB_000569"
     CDS             complement(4152..6023)
                     /gene="gsiA"
                     /locus_tag="ACHUPB_000569"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0757470.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="ACHUPB_000570"
     CDS             complement(6010..6975)
                     /gene="iaaA"
                     /locus_tag="ACHUPB_000570"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0757471.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7179..8414
                     /gene="moeA"
                     /locus_tag="ACHUPB_000571"
     CDS             7179..8414
                     /gene="moeA"
                     /locus_tag="ACHUPB_000571"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0757472.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8414..9163
                     /gene="moeB"
                     /locus_tag="ACHUPB_000572"
     CDS             8414..9163
                     /gene="moeB"
                     /locus_tag="ACHUPB_000572"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0757473.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="ACHUPB_000573"
     CDS             complement(9342..10004)
                     /gene="fsa"
                     /locus_tag="ACHUPB_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0757474.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10135..11034
                     /locus_tag="ACHUPB_000574"
     CDS             10135..11034
                     /locus_tag="ACHUPB_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0757475.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11040..13472
                     /locus_tag="ACHUPB_000575"
     CDS             11040..13472
                     /locus_tag="ACHUPB_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0757476.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13618..14433
                     /gene="ybiV"
                     /locus_tag="ACHUPB_000576"
     CDS             13618..14433
                     /gene="ybiV"
                     /locus_tag="ACHUPB_000576"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0757477.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14585..15850
                     /locus_tag="ACHUPB_000577"
     CDS             14585..15850
                     /locus_tag="ACHUPB_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0757478.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15817..16977)
                     /locus_tag="ACHUPB_000578"
     CDS             complement(15817..16977)
                     /locus_tag="ACHUPB_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0757479.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17215..18516
                     /locus_tag="ACHUPB_000579"
     CDS             17215..18516
                     /locus_tag="ACHUPB_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0757480.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18531..20894
                     /locus_tag="ACHUPB_000580"
     CDS             18531..20894
                     /locus_tag="ACHUPB_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR0757481.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21074..22669)
                     /locus_tag="ACHUPB_000581"
     CDS             complement(21074..22669)
                     /locus_tag="ACHUPB_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0757482.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22888..23808
                     /gene="ldtB"
                     /locus_tag="ACHUPB_000582"
     CDS             22888..23808
                     /gene="ldtB"
                     /locus_tag="ACHUPB_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0757483.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23867..24985)
                     /locus_tag="ACHUPB_000583"
     CDS             complement(23867..24985)
                     /locus_tag="ACHUPB_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0757484.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="ACHUPB_000584"
     CDS             complement(24982..25449)
                     /gene="mntR"
                     /locus_tag="ACHUPB_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0757485.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25635..25763
                     /gene="mntS"
                     /locus_tag="ACHUPB_000585"
     CDS             25635..25763
                     /gene="mntS"
                     /locus_tag="ACHUPB_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0757486.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26035..27618
                     /gene="opgE"
                     /locus_tag="ACHUPB_000586"
     CDS             26035..27618
                     /gene="opgE"
                     /locus_tag="ACHUPB_000586"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0757487.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="ACHUPB_000587"
     CDS             complement(27667..28182)
                     /gene="ompX"
                     /locus_tag="ACHUPB_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0757488.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28235..28300)
                     /locus_tag="ACHUPB_000588"
     CDS             complement(28235..28300)
                     /locus_tag="ACHUPB_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757489.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28535..29422
                     /gene="rhtA"
                     /locus_tag="ACHUPB_000589"
     CDS             28535..29422
                     /gene="rhtA"
                     /locus_tag="ACHUPB_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0757490.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29721..30224
                     /gene="dps"
                     /locus_tag="ACHUPB_000590"
                     /gene_synonym="pexB"
     CDS             29721..30224
                     /gene="dps"
                     /locus_tag="ACHUPB_000590"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0757491.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30628..31374
                     /gene="glnH"
                     /locus_tag="ACHUPB_000591"
     CDS             30628..31374
                     /gene="glnH"
                     /locus_tag="ACHUPB_000591"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0757492.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31513..32172
                     /gene="glnP"
                     /locus_tag="ACHUPB_000592"
     CDS             31513..32172
                     /gene="glnP"
                     /locus_tag="ACHUPB_000592"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0757493.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32169..32891
                     /gene="glnQ"
                     /locus_tag="ACHUPB_000593"
     CDS             32169..32891
                     /gene="glnQ"
                     /locus_tag="ACHUPB_000593"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0757494.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33008..35233
                     /gene="ybiO"
                     /locus_tag="ACHUPB_000594"
     CDS             33008..35233
                     /gene="ybiO"
                     /locus_tag="ACHUPB_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR0757495.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="ACHUPB_000595"
     CDS             complement(35230..36156)
                     /gene="rlmF"
                     /locus_tag="ACHUPB_000595"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0757496.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36432..36692
                     /gene="mcbA"
                     /locus_tag="ACHUPB_000596"
     CDS             36432..36692
                     /gene="mcbA"
                     /locus_tag="ACHUPB_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0757497.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36957..>37257
                     /locus_tag="ACHUPB_000597"
     CDS             36957..>37257
                     /locus_tag="ACHUPB_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0757498.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9032 a        10058 c         9338 g         8829 t
ORIGIN      
        1 caaatcgtga tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg
       61 atcaaagcaa ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca
      121 cggtcgtttc gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg
      181 tccatttact tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag
      241 ataatattta ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat
      301 tttcagaatc acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt
      361 taatgtcagg gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag
      421 ttcaaagcgg ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat
      481 gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt
      541 tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat ctgaacgctg
      601 tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
      661 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      721 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      781 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      841 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc cccactctgg
      901 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      961 gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca agatatgacg
     1021 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
     1081 tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg aaaaaatggc
     1141 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
     1201 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcagcc gatcccacca
     1261 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
     1321 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
     1381 gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
     1441 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tggccaccac
     1501 aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc gaaatcgtcg
     1561 ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca tggcgtttaa
     1621 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1681 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1741 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1801 ccaagtccgg gccagttgaa aactttctcc accacgatag aaccaccgag caaaaagcca
     1861 aactgtaagc ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1921 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1981 acatcgacaa acgacgcgcg ggtaaagcgc gccatcacgg ctgccactgc cgcgccgagc
     2041 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     2101 agttcaacgg agaatacctg aattaaaagc atccccagcg caaatgccgg aaacgagatc
     2161 cccgacaccg caatggtcat actcaatcga tccggccaac gattacgcca gacggcggcg
     2221 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     2281 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     2341 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     2401 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgagcccg
     2461 gcaatcaatc gcgccggatc gccgggcagc atatgcacaa ataaaaacac cagcaccgag
     2521 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     2581 ctccctgcgt tgcttattgc aaatccgcgt cttcaaagct gaagccggtg tctggcatga
     2641 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2701 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2761 ccggatcatt agttttcagt gcctgagcca ggaagtcgtc cacctgttta ttgctgtaaa
     2821 atgcggtatt aaacagcgtc ggtggccagt tttgcgaggc gaacagtggc gatagcgccc
     2881 agtccgcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2941 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgcg gtcacctggg
     3001 ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     3061 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     3121 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     3181 tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca actttcacca
     3241 gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     3301 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     3361 tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa gcaaactgcg
     3421 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     3481 tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc accttcacaa
     3541 aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga tgaaaaccaa
     3601 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3661 gaatattaat aaacgctgag aacggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3721 tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3781 cgctggcccg gtccagattc gcttttaccg ccactgcgtt gaaatcggtg ccatcctgga
     3841 atttaatgcc ttcccgcaat ttcacggtgt aagtaatgcc gtcatcggaa acggtataac
     3901 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3961 acgatttcgc tacggcctga gataacgtat cattcgcgtc atacggatcg agcgtggtga
     4021 aattcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat gccatcaacg
     4081 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agcttttgcc attttattct
     4141 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     4201 cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag atgaatattg
     4261 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     4321 ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa gaccgcgcgc
     4381 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     4441 tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     4501 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga aacggcttcg
     4561 tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4621 tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag gcccacgcgc
     4681 tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc gtgtacacgc
     4741 agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4801 tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga caaggtatcg
     4861 attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4921 cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcgccaggc
     4981 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     5041 ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg ttcgccatcc
     5101 accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag cgatatcaac
     5161 gggaaacgtc ggggataatc caaccctttc atcgcaccaa gttgcggaac agcagctaac
     5221 agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt acccgtttcc
     5281 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacgcccata
     5341 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt gattaattgc
     5401 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     5461 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     5521 tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg atccagcatc
     5581 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aattgattcg
     5641 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5701 atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc gctaagttca
     5761 atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa accgcccgcc
     5821 tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc ggattcgcca
     5881 acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac cgcagctatt
     5941 ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac attaccggca
     6001 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     6061 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     6121 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     6181 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     6241 gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccgatac aagaaaccgc
     6301 cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc caactcgtcc
     6361 gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc cgtctaaatc
     6421 caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     6481 gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac gcaaaggcgt
     6541 ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat tttctgcccc
     6601 ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg caagaaccgg
     6661 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6721 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6781 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6841 cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa tgtagcgtaa
     6901 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6961 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     7021 cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat atggcatgta
     7081 aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     7141 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     7201 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg accgcccagg
     7261 aaacgctgcc actggtacag tgttttggtc gtattctggc gagcgatgtc gtttcgccgc
     7321 ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt ttagccgata
     7381 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtatcatg
     7441 gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     7501 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     7561 ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7621 ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt gcttcactgg
     7681 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7741 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc aaccgtctcg
     7801 ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg attatccgcg
     7861 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7921 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7981 tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc gcgttcggta
     8041 aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     8101 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc agcggcctgc
     8161 ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga cgtcttgatt
     8221 tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg accaccggac
     8281 atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc gtgctggaac
     8341 gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt aacgcgttgt
     8401 tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca accggcaaat
     8461 cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc gtgtgctgat
     8521 cgtcggcctc ggtggactcg gctgcgcagc ttcgctgtat ctggcaagcg ccggtgtcgg
     8581 caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac gccagacact
     8641 gcacagtgat gccacggtcg ggcaaccgaa ggtggaatcc gcccgtgacg ccctgacgcg
     8701 gatcaaccca catatcgcga ttacgccagt caatgcactg ctagatgacg cagaacttgc
     8761 agcattgatt gctgaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8821 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8881 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcgctgcct
     8941 tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga tggcaccgtt
     9001 gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg caggttatgg
     9061 caaaccgacc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt ttcgcgaaat
     9121 gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac ctccaggcct
     9181 gataagacgc gacaagcgtc gcatcaggca tttgctccag acgccagatg cggcgtgaac
     9241 gccttatctg gcctacggtt cgggcacaga tttccaagcg cggcaggcat cggtgcactc
     9301 cgccacgtca tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca
     9361 aacgctccct gccagtcctg ctcaaacttc gccacagcgg cttccaccgc cggatagcta
     9421 atcatctgtt gtgccacatc cagtggcaga gtaattgatt cacatcctgc cagtaagcag
     9481 tccagcgcct gacgcggggt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc
     9541 attttcaata actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca
     9601 atacgattaa cgtaaggcgc aacatattcc gcacctgcca gcgccgacag caacccttgt
     9661 gctgcgccat ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata
     9721 gctgccagcc cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc
     9781 aatttacgtg cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga
     9841 cgcccctgac cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta
     9901 cccgcggcga taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc
     9961 gccttcaccg caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg
    10021 tactttttgt ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca
    10081 atctaacttt cattcgaaag tattttaatg tttgaacgaa acagagaggc gactatgatt
    10141 ttcaatattc agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt
    10201 aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag
    10261 gatctgctgt atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggccgcg
    10321 ccggaagtga ttgagcgcgc gctgaatggt ttgcttattc atcgggaaaa gttaaccccg
    10381 gagcatctga cggcgttaac cgactgctgt ccgacacagg cattaaccgt gtgtggtgaa
    10441 gtgaaaagcg ttgaggagat catgacgacc gttctgcgcg ataaaccgtt ttacgatcgc
    10501 agcggcggcg gtttaacgct ttcgggtggt gagcccttta tgcagccgga aatggcgatg
    10561 gcgctactgc aagccagcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat
    10621 gtgccgtgga aatatatcgc cccttctctg ccctatatcg atctgtttct tgccgattta
    10681 aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc cagagtgctg
    10741 gataacctga aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt
    10801 cagggcttta atgccgacga aacctctgta aaagctatta ccgattttgc cgccgacgag
    10861 ctgcacgttg gcgaaattca tttcctgccc taccacacgc taggcatcaa caaatatcac
    10921 ttacttaatc tgccctatga cgccccggaa aaaccgcttg atgcgccaga actgctcgac
    10981 tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca
    11041 tgaccacact gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg
    11101 tgcatattgt gaaaccgcca gtctgtaccg agcgcgcgca gcactatacc gagatgtatc
    11161 aacaacatct cgataagccg atcccggtac gtcgcgcgct ggcactggcg catcacctgg
    11221 cgaatcgcac catctggatc aaacacgatg agttgatcat tggcaaccag gcaagcgaag
    11281 ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg gatcgaaaaa gagattgatg
    11341 atctggcaga tcgtcccggt gctggctttg cggtgagcga agagaacaaa cgcgttctgc
    11401 atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga tcgctgctac ggcatgttta
    11461 ccgatgagca aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct
    11521 ccggcgatgc gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc
    11581 tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc
    11641 acggcgaaca gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg
    11701 aacgttttgc agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg
    11761 atgaactgct ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt
    11821 tctggcaggc gctgcaactg tgttacttca tccaattgat attgcagatc gaatctaacg
    11881 gtcactcggt atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgcgacg
    11941 ttgaactcaa ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc
    12001 tgaaactgct ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa
    12061 gtcctctgta tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg
    12121 acgcggtgaa tccactctct tacgctatcc tcgaatcctg cggtcgcctg cgttccactc
    12181 agcctaacct cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg
    12241 tacaggtgat ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc
    12301 cggaatttat taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt
    12361 gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca
    12421 acttcgcccg cgtgatgctg gcggcgctgg aaggcgggcg tgatgccacc agcggcaaag
    12481 tgttcctgcc acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa
    12541 tggacgcgtg ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg
    12601 tcgtcgacac catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg
    12661 actgtattga gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg
    12721 gcctgcaggt tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat
    12781 ttgaacaggg cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg
    12841 gcctgactca tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg
    12901 acgatgatac cgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga
    12961 aacagtacca taacccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta
    13021 cctcgtctat ctccgctaac gtaccgtttg gcgcgcaaac tatggcaaca ccggacgggc
    13081 gtaaagccca caccccgctg gcagaaggcg caagcccggc ctccggtact gaccatcttg
    13141 gccctactgc ggtcattggc tcagtgggta aactgcctac ggcagcgatt ctcggcggcg
    13201 tgttgctcaa ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaaac
    13261 tgatgatcct gctgcgtacc ttctttgaag tgcataaagg ctggcatatt cagtacaaca
    13321 tcgtttcccg cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg
    13381 tagtgcgtgt cgcgggctat tccgccttct tcaccgcgct ctctccagac gcacaggacg
    13441 atatcatcgc ccgtactgaa catatgctgt aatctcctcg gcccggcggc gtcgtcgccg
    13501 ggctaaatca ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct
    13561 ctgcgtcaca ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg
    13621 agcgtaaaag ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac
    13681 aaccaaccac gttttatggc gcaatatcag gaactgaaaa agcgcggcat taagttcgtt
    13741 gtcgccagcg gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag
    13801 atctcttttg tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac
    13861 ggcgaactga cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa
    13921 ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca
    13981 tttgtcgcac tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag
    14041 attgacgacg tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg
    14101 atcgacaaac tgcacatagc gctcgatggc attatgaaac ccgtcaccag tggttttggc
    14161 tttatcgacc tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa
    14221 cgctgggatc tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag
    14281 atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa
    14341 atcgcccgtt acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg
    14401 gtgctggata acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg
    14461 ttttcccttc tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat
    14521 ttataactta tattgtcgca tgcaaatcag aatggtttcg aatgaaagtt aatgagaggt
    14581 ggttatggct tctactttta ccagcgacac attgcctgcc gatcacaaag cagctatccg
    14641 tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct
    14701 aagcgatgac attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc
    14761 cgtctggccg gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg
    14821 cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc catttccccc gcgaacaagc
    14881 gctaggctgg gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta
    14941 caaaggcccc ggcgataatt tcttcgggac gctcagcgct tcacgtcccg agatttaccc
    15001 catctactgg tcgcaggcgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca
    15061 gtcgtttctc aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga
    15121 tgtgagcgtc atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg
    15181 tcttggagcg cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg
    15241 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    15301 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    15361 attccaaggc tggacagcgc tctctaatat gctgcctggt caggggctgc tgcacgttgt
    15421 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    15481 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    15541 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    15601 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    15661 gatgtacatt cctgcggcac caatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    15721 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    15781 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggaat
    15841 cgacagctga gtgtcgtaca gcccttcccg ttccacggcg gtgttccctt ttcctgggcg
    15901 aacccgccgc actgactccc gcaccgccaa tcgtccgtgc agcagcagcg agcccaccgg
    15961 agcatccggg cgaatcagcc gctgttgcaa catttgcacc gcttcattgc ctagctcatc
    16021 gcgtggcaca tgcacagccg tcaggggaac atcctcgatt gccgcaaggt taaagccgtc
    16081 gatactcatc accgagacat cctgtggtac gcgcagacca cgacgatgta gcgcatttac
    16141 cgttcccgcc gccataaaat ccccgccgac cagaaacgcc gtcggcaacg tcttgccatt
    16201 ggcggcatcc agccacgctc cgaccttttc ttccgcttct ttagcgctaa aactgctgat
    16261 attcagcaaa tgacggtcat ggtgaaaggc cagattatgc tgacgccatg attctttaat
    16321 ccctgcaaac cgctgctcca tagtgtagcg gcgcaggcac atcacattca tcacctcccg
    16381 gtgacccatt tcaaacagat agcgggcggc gaacgcgcca attaaccgat gatccggcgc
    16441 aatcgccggg aggcgcattc gttcatcacg gcagttgatc agcacacaag gcttagcgag
    16501 atcggcggcc agatcatgga tgtgcggatc gtcaatcccc agcaggatcg ctgcctgcgt
    16561 atctgcttca ttcatccgcg agagaaagag cgcgggatcg ctgtcaaact cctccagcgc
    16621 gcagtaacgc agccgcactt catgcggcga taacgctttg ctgatgctct ggatcacacg
    16681 gtaataaaag atatcagatc gttggtcaaa agcgcgttgc ggcgcgaaaa taatcaggtt
    16741 gttcagcaac atacgacctg ctgccatgcc atccatcacg cccaactcac gcgcgcaggc
    16801 cagcacctgt tcgcgagcgc gtgcactggt attcgctttg ccagccagca cccgcgaaac
    16861 cgtactcggc gatagcccgg tacgtgcggc gatatcggcg attttcagct tcttgtccat
    16921 tttgtgatct gcctctaatt cggaaatgac agaattttca tgagcaaaaa agcccattct
    16981 gtctggcatt aaatccattt cagtaatgta tatcagtcac tcatggaaaa acgtgcacaa
    17041 aacccgcttt ctttcgcttt tagggtctct tactgtgaat tggtagaaca acttccatac
    17101 aaaaataatg gaaaaacgaa caataaagac tttatttatc aaaaagttaa aagtggtgta
    17161 acaactccat tctgacgttg ccgcacaaac cctataccat tctgtggagt gcatatgagt
    17221 cagggtatca ataacgatat gactgccagt aaaccgcgcc atatcgtcaa aaatctgcgt
    17281 tggtggatac tggtcctttt tttgctcggt gttactgtca actacatcac gcgtaactcg
    17341 ctgggtattc tcgcccccga actgaaaagc agcctaggga ttaccaccga acaatattcc
    17401 tggattgtag gtgctttcca gctcgcctat accctattcc aaccgttgtg cggatggctg
    17461 attgacgtca ttggcctcaa actgggcttt atgatctgcg cgggactgtg ggcactggcc
    17521 tgtctgatgc atgcgggggc gggaaactgg ggacatctgg cgctgttgcg cttctttatg
    17581 ggcggtgcgg aagccgcggc aaccccagca aacgccaaaa ccatcggtga atggtttcca
    17641 aaatcagaac gcccgatcgc cgcaggctgg gcaggcgtcg gtttttccat cggtgccatg
    17701 ctggccccac cgattatcta ttttgcccac gcttcattcg gctggcaggg tgcatttatg
    17761 ttcactggca tcctggcgat gctgtgggtg gtgctgtggt gggcgttcta tcacagcccg
    17821 gataagcatc ctaacctcag caaagccgag ctggacttta ttcgccagga taacgaagcg
    17881 cccccggtca agttaccctt tctgaccgca ctgaaaaccg tgggcaaaaa taaacgcttt
    17941 tacggtatcg ctatcccggc ctttatggca gaaccggcgt gggcggtgat gagtttctgg
    18001 gtgcctctat acctgtccaa aacctatggc atggacctga agcagatcgc attattcgcc
    18061 tggttgccgt tcctcgccgc tgatcttggc agtattgcca gcggctatct gaccaaactt
    18121 tacacccgcc tgtttgggct gagtcgtgtg aactccgttg tcgcatcatc ggtcagtggc
    18181 gcattcttga tggtttcgct ggcggtgatg gcctttacca ataacccgta tatcgccatc
    18241 gtccttatct ccatcggcgg cttcggtcac cagatcatct cctgcatgct gagcgcactg
    18301 gtggtggaat ccttcgacaa aggtcagatg gccactgtta acggcatgcg cggttctgcc
    18361 gcgtggatcg caagtttcct gttctccctg attatcgggg ttaccgctga caaaatcggc
    18421 tttaaccccc tgtttatcgc catgggcttc tttgacctga ttggcgcttt cttcctggtc
    18481 acctttattg ctgaacgtcg cgcaaaacgc gcctgagagt ggatgtaaac atgaaaacgc
    18541 tgaaaaactg gaccctgcac aaacagtctg cccaccacgt tgaactgctg gtcgatggcc
    18601 agcatatcct gtgcctgtac gtgctggaag aaaatctgtt ccgcgtgctg ttaaaacgca
    18661 aaggcgagct ggcgctagat cgcacctgga gcattgctcc ggcgcaggat gtgccatggg
    18721 aagggcgcag ccgcgaagat ctcagcggct ttagcctgcc cgcgtggaca gtgatccagc
    18781 acgacgacac cgtggttatc gccaccgagt cactgcgtgc aaccgttcac cagccgctgt
    18841 ggctggagtg gcactatcgt aacaacgaag gcgaatggct gccgttggtg aatgaccgcc
    18901 cgaccagcgc ttatctgctg aatgcccacg gcgatggcgt ggcacactat cagagccgtc
    18961 gtaaagatga acgcttttat ggcctcggag ataaagccgg cgatctgcaa cgcaacggta
    19021 aacgttacga gatgcgtaac ctcgacgcga tgggctacaa cgccgttagt actgacccgc
    19081 tgtacaaaca cattccattc accatcaccc gccgcgatga cattagcttc ggcctgtttt
    19141 atgacaacct cagcagctgc tggctggatc tgggcaacga gatcgacaac taccacaccg
    19201 cctatcgccg ctggcaggcg gaagcgggtg atatcgacta ctacctgttt accggcgaac
    19261 gcgtacttga tatcaccaaa gccttcgtac gcctgaccgg aaaaaccctg ttcggaccga
    19321 aatggagcct gggctacagc ggttcaacta tgcactacac cgatgcgccg gacgcacaaa
    19381 atcagttgat gaactttatc cgcctgtgtg aagaacatgc aattccctgc gattcgttcc
    19441 agctctcttc cggttatacc tcaattaacg gcaaacgcta tgtgttcaac tggaactacg
    19501 acaaagtgcc gcagccgaaa gtgatgagcc aggcatttca caatgctggt ttgcacctgg
    19561 ccgccaatat caagccgtgc ctgttgcagg atcacccacg ctataacgaa gtggcagaaa
    19621 gaggactgtt catccgtgat tcagaaacag atgaaccaga acgttccagc ttctgggatg
    19681 acgaaggttc taacctcgag tttactaatc cgcagaccat ccagtggtgg caggaaggcg
    19741 tgaccacaca gcttctggag atgggtatcg actccacctg gaacgacaac aacgaattcg
    19801 aagtatggga cggtgaagca cgctgtcacg gctttggtca gacgatcgcg atcaaacata
    19861 ttcgcccagt gatgccactg ctgatgatgc gagcctcgat ggaagcccag cagcgtttcg
    19921 ctccggaaaa acgtccgtac ttgatctccc gctccggctg cgcgggaatg cagcgctacg
    19981 tgcagacctg gagtggcgat aaccgcacta gctgggacac tcttcgctac aacacccgca
    20041 tgggtctggg gatgagcctc tccgggctgt ataacctcgg tcacgacgtc ggcggtttct
    20101 ccggtgataa gccggacccg gaactgtttg tacgctgggt acaaaacggc gtgatgcatc
    20161 cgcgctttac gattcactca tggaacgatg accataccgt aaacgaaccg tggatgtatc
    20221 cgggcgtcac tccggcgatc cgcagcgcta ttgagttgcg ctatcgcctg ctgccctact
    20281 tttataccct gatgtggcag gcatacgccg atgatgaacc aatgctacgt ccgactttcc
    20341 ttgaccacga gcacgacgtt cagacttttg aagagtgtga tgacttcctg cttggtcgcg
    20401 atattttggt tgccagcgtc gtagagcaag gcgagcgcca gcgccgtgta tggctgccag
    20461 ataacgaaac tggctggtac gatttctaca acggcgaatg gttcagtggc ggtcagtgga
    20521 tcaccctcga cgcaccgctg gagaaactgc cgctgatggt tcgtgccggg gctggtctac
    20581 cgctcagcaa acgcatcacc tatgtgagcg cagaacaaga cgatacccgc gaactgaaac
    20641 tgttcccgct gaaaggtgtg ggcacaacgt ccggcctgct gtttgaagac gatggcgaaa
    20701 gctggggcta ccaaactggt aatgcgcttt gggtggaatg ggaaatggtt tgcgatgggg
    20761 cgaccatcaa cctgaaagtc aacgcgcgcg gggattatcg tcctgcatgg agtgcactga
    20821 aagtgtcatt accattggaa gaaaaacgca cgctgctggt caatggtgtt gaagggagtg
    20881 aatggatgcg ttaaatcatt accggggccg ggaggcgact gcaccttacc cggtccagcc
    20941 atcgaatcct ttacctcagt atttcacgct gcaggaaggc agcaaacctg taaaccccgg
    21001 gagcttaaat aagtaagtga ccggggtgaa caggtgaagc caatgcatct gccgcatgaa
    21061 agacggcggg gaattaagcg tcgatccctt tactacgcag gtaatcttcg taattaccgc
    21121 taaagtcgat cgcgcgttcc ggggtgattt ccagaatgcg ggtcgccagg gagcttacga
    21181 actcacggtc gtgtgaaaca aagatcagcg tgccctgata cagttccagc gccatgttca
    21241 gtgactcgat ggattccata tccaggtggt tggtcggttc gtccatgatc agaatgttcg
    21301 gcttctgcat cattaactta ccaaacagca tccgcccttt ttccccaccg gaaagcactt
    21361 tagctggctt tttgatgtcg tcctggctga acagcaaacg accgagaatg ctgcgtaccg
    21421 cctgctcgtc atcgccttcc tgcttccact gactcatcca ttcgaacacg gtcagatcat
    21481 tttcaaactc atattcgtgg tcctgggcgt agtaaccaat gcgcgcgttt tcagaccatt
    21541 ttacggtgcc gctgtccggt tgcagatcgc ccaccagcgt tttcagcagc gttgatttac
    21601 cgacgccgtt ggtacccagt accgcaagtt tttcaccgac ctccagcagc aggttgagat
    21661 ttttaaacag tggaccgtta tcaaaccctt tggtcagacc ttccacttcc agcgcgttac
    21721 ggaacagttt cttatcctgt tcaaaacgga tgaacgggtt ctgacggctg gaggctttca
    21781 cctcttccag tttgatttta tcaatctggc gcgcgcgcga agttgcctgg cgagatttcg
    21841 aggcgttggc gctaaagcgg ctaacgaaag attgcaactc agcaatctgc gctttcttct
    21901 tggcgttatc ggccagcaga cgttcacgcg cctgggtcgc cgccgtcatg tactcatcgt
    21961 agttacccgg ataaacgcgc agctcgccgt aatccagatc cgccatgtgg gtacagacca
    22021 tgttaaggaa gtgacggtcg tgcgagatga tgatcatggt gctgtcacgc tcgttcagca
    22081 cctgttccag ccagcgaatg gtgtcgatgt cgaggttgtt ggtcggttcg tcgagcagga
    22141 gaatctccgg atcagcaaac agcgcctgcg ccagaagcac acgcagtttc cagccaggag
    22201 caacttcact catcgggccg tagtgctgtt ccactggaat tcccacgcca agcaacagtt
    22261 caccggcgcg agcttccgca gagtaaccgt ccatttcgcc gtatttaact tccagatcgg
    22321 ccactttata gccgtcttcc tcactcattt ccggcaaagc atagatgcgg tcacgctcct
    22381 gcttcacttc ccacaactct ttatgcccca tgatcaccgt atccagcaca gtgaactctt
    22441 caaaggcaaa ctgatcctga cgcagcttac caatgcgctc gttgggatcg agggaaacgt
    22501 tacccaaagt cggctctaag tcgccgccga ggatcttcat aaaggtggat ttaccactac
    22561 cgttcgcgcc aatcaggccg taacggttgc cgccgccgaa tttgacggaa atgttttcaa
    22621 acaacggctt actgccgaac tgcatggtga cgttactgga aactaacaca ggcgtatcct
    22681 gaaaagagat atgacaaacc gcgcattatg ccataagtct gaaagaagat cctgctcctc
    22741 gcttaaggtc tacactcaag ccaaaggcaa aaaatgtgat ttcgtacaca tctgatttca
    22801 ctgtgagctg gaatgaactt ataatgcgct tccaatactc taatattctc aacccaatgg
    22861 cctgccaggc acaaaatctc gcttaacatg aatatgaaat tgaaaacatt attcgcagcg
    22921 gccttcgctg ttgtcggctt ttgcagtacc gcctctgcgg taacttatcc tctgccaacc
    22981 gacgggagtc gcctggttgg tcagaatcag gtgatcacca ttcctgaagg taacactcag
    23041 ccgctggagt attttgccgc ggagtaccag atggggcttt ccaatatgat ggaagcgaac
    23101 ccgggtgtgg ataccttcct gccgaaaggc ggtactgtac tgaacattcc gcagcagctg
    23161 atcctgccgg ataccgttca tgaaggcatc gtcattaaca gtgcagagat gcgtctgtat
    23221 tactatccga aagggaccaa caccgttatc gtgctgccga tcggcattgg tcagttaggc
    23281 aaagatacgc ctatcaactg gaccaccaaa gttgagcgta agaaagcagg cccgacctgg
    23341 acgccgaccg ccaaaatgca cgcagagtac cgcgctgcgg gcgaaccgct tccggctgtc
    23401 gttccggcag gtccggataa cccgatgggg ctgtatgcac tctacatcgg tcgcctgtac
    23461 gctatccatg gcaccaacgc caacttcggt atcggcctgc gtgtaagtca tggttgtgtg
    23521 cgtctgcgta acgaagacat caaattcctg ttcgagaaag taccggtcgg tacccgcgta
    23581 cagtttattg atgagccggt aaaagcgacc accgagccag acggcagccg ttatattgaa
    23641 gtccataacc cgctgtctac caccgaagcc cagtttgaag gtcaggaaat tgtgccaatt
    23701 accctgacga agagcgtgca aacagtgacc ggtcagccag atgttgacca ggttgttctt
    23761 gatgaagcga ttaaaaaccg ctccgggatg ccggttcgtc tgaattaatc ttcaaaactt
    23821 aaagcaaaag gcggactcat aatccgcctt ttttatttgc cagaccctag ttggccggga
    23881 gtataactaa caaaacatat cccaccaacg ccgcccacaa cagcatcggt attgaataga
    23941 gatggaaacg ccaccagatg cggcgatcgt tcgccatacg tagcgcaatc aaatttgcca
    24001 gcgatccggg taataaccca aagccaccta cgtttaccgc ccataccagt aataaagacg
    24061 gcggcacata gttcagcaac aatatggtac tcggcacgtt actgatcacc tgcgataaac
    24121 cgattgccgt taaccataac ccgggttcag atagatgact cacgttaccc aacacgcctt
    24181 gcaacgctgg aagctgggtc agtaaatgga cgtcgataaa catcgccata aacaccagca
    24241 gcagcgtcca gtccacactg agcaccacgc gacgtgccag cagcgcaaag cctgccgcca
    24301 caatcaccag tccccacagc tcttgtttga actccagcgc cgtcagaaag acgatataca
    24361 gccccagaca actccacacc agccgcggtt tccactccgg tgtttgcacc cccgtatggt
    24421 attgcagcgc ctttccaggg aaacaacacc agcacaggag caggagcgtc agcatcattg
    24481 cgccagccag cggtgccatt tgggcaataa atccggcaaa cgaaagacca gaacgtcccc
    24541 agataagaat attttgcggg ttgccaattg gcgtcagtag cgaaccagcg ttgactgcca
    24601 gcgcctcaaa aataatcagc cgattaaccg ggatctcaca cagtcttttt agcgtgatag
    24661 tcagcggaac aacaataaac agcgcgacat cgttggtcag aaaggtagaa agcagcgccg
    24721 ccgccagcac cataaacatc gccagccgac gctccgtagc aaagcggcgc accattttgc
    24781 gccccagcac atcaaaataa ccgcttaact ccacaccttt ggtcagcagc atcaggccgc
    24841 ttaaggtgat gatggtgtgc cagtcgatag cagcaggcca ggatttcggt gcaaagggca
    24901 cgaaaaagct taatccgata ccaacaagaa ttaataactg aaaaaaacga tcgccttgca
    24961 gcgtgcgtaa aaaaggcagg ctcatttggc accgtgtttc tgggtaaaca aacgaaaagc
    25021 gtcgagcgtc tcttcactaa catggtgctc catgccttcc gcgtcgcgac gggcgatttc
    25081 cggactgacg cccaacacca acaagaaatt ttcgactatc tgatgtcgct cgcggctttc
    25141 ctgcgccagc ttctctcctt ctgccgttaa aaacacgcca cgccagggga tcatttcaat
    25201 cagccccatg gttgccagcc gcttaagcat tttagccacc gtcggttgcg aaactcccag
    25261 acgagcagcc atgtccacct gacgagcttc ccccacttcc ctgatcaagt cagaaatcag
    25321 ctcaacgtaa tcatcaataa gctcgcgccg atgcgcctct ctgacctggc ggaacccttc
    25381 aacgtgctct tccacgttca ctaactgcgt cacttttttt gctgttggcg tacctgcgcg
    25441 acgactcatt gtgcttcctc attatggtga cgcatacata gcgttctcat ttcgtgcgca
    25501 cattgtaaac cagagttgcg aaggtacaaa aaattaacgt tttagcaata gctacataat
    25561 atagcctgtg ctatatctgt atgtaatgca atcatccctc aaggatcgac gggattagca
    25621 agtcaggagg tcttatgaat gagttcaaga agtgtatgcg cgtgtttagt cattctccct
    25681 ttaaagtacg gttaatgctg ctctctatgt tgtgcgatat ggtcaacaac aaaccgcagc
    25741 aagataaacc ttccgataaa tagcggcgtc gcggtacgcc gcttcactcc tgcttttatg
    25801 caggcataac gcgttttggt ctgaaaaacc ccactttttg tcggatttgc aatccccttc
    25861 gcaaaagatt tgttcgtcag tagttgacct gaacggcggc tcgctctatc ttcttgcagc
    25921 cctacgtata ttgcggctcg cggatgcgga cccctttccc ctcttcacgc actcttgcag
    25981 gtattgaccc ttgacgccag ggtaagcaca tggcgtttgt tacgatagtg gcatatgaat
    26041 ttaaccctca aagaatcgct tgttacccgt agccgggtat ttagcccgtg gactgcgttc
    26101 tactttttac agtcgctatt aattaacctc ggcttaggtt accccttcag tttgctctac
    26161 accgctgcgt ttacggctat tttgcttttg ctatggcgaa cattgcctcg cgtacaaaaa
    26221 gttctggtcg gtgtcagttc gctggtggcg gcttgttatt tcccttttgc tcaggcctac
    26281 ggcgcgccta atttcaatac attgctggca ttgcactcca ccaatatgga agagtcgacc
    26341 gaaatcctga cgatttttcc gtggtacagc tacctggtcg gcttatttat ttttgcgctc
    26401 ggcgtaatag caatcaggcg aaaaaaagag aatgaaaaag cgcgctggaa taccttcgac
    26461 agcctgtgtc tggtattcag tgtggcgaca ttttttgttg ctcccgtgca aaacctggcc
    26521 tggggtggcg tatttaaact gaaagatact ggctatccgg tatttcgttt tgctaaggat
    26581 gtcatcgtca ataataacga ggtgattgaa gagcaagaac ggatggcaaa actttccgga
    26641 atgaaagata cctggacggt cactgccgtt aagccgaagt atcagaccta tgtggtggtg
    26701 atcggcgaaa gcgcgcgtcg cgatgccctc ggtgcctttg gcggtcactg ggacaatacc
    26761 ccgtttgcca gcagcgttaa cggtttgata tttgctgact acattgccgc cagtggctcc
    26821 acgcagaaat cacttggctt aacgctcaat cgcgtagtcg atggcaaacc acagtttcag
    26881 gataactttg tcaccctggc aaatcgcgcg ggcttccaga cctggtggtt ttccaaccag
    26941 ggccaaatcg gcgaatacga tactgctatc gccagcatcg ccaaacgtgc agatgaagtg
    27001 tacttcctga aagaaggtaa tttcgaagca gataaaaaca cgaaagacga agcgttactg
    27061 gatatgaccg ctcaagtgct ggcgcaagag cactcgcaac cgcagctgat tgttctgcat
    27121 ctgatgggct cgcatccgca ggcctgcgac aggacacaag gcaaatacga aacctttgtg
    27181 caatcgaaag aaacgtcgtg ctatctctat accatgacgc aaacggacga tttactgcgc
    27241 aagctgtacg atcagttacg caacagcggc agcagcttct ctctggttta cttttctgac
    27301 cacggtctgg cctttaaaga gcgcggcaaa gacgtgcaat accttgccca tgatgataaa
    27361 tatcagcaaa atttccaggt gccttttatg gtcatttcca gcgacgataa agcgcatcgg
    27421 gtgattaaag cccgccgctc agccaatgac ttcttaggct ttttctcgca gtggacggga
    27481 attaaagcga aggaaataaa tatcaaatac ccgtttatat ctgagaagaa agccgggccg
    27541 atatacatca ccaacttcca gttacagaag gtggattaca accatctcgg aaccgatatt
    27601 ttcgacccga aaccttaaaa caaaaaatcc gccccgagag gcggattttt tatatcacca
    27661 aagtgattag aagcggtaac caacaccggc aatccaggtg cctacgtcaa cgctacgaat
    27721 acggctctgc tcgtaagaga agtccagagc aacgttttcc atcgggttga actgcagacc
    27781 agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg taggtcgggt attcagtggt
    27841 ctggaattta ccataaccca cccccactac accgtagatg cttgcccagt cgttaatgcg
    27901 gtaagccgga ccagcagtga tgccgtagta ctggtttttg ttgtagtcac cagagcttgc
    27961 agtacggctt ttctcggtgt aagtgaaaga accgatcaca cccagcgggc tgttgtcttc
    28021 ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc atttggccct gagcgtcgct
    28081 ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg gaagtacctg cggtgaaagc
    28141 cagaactgcg gccagtgctg aaagacatgc aatttttttc ataaccacct caaatgtgat
    28201 tcaaataagt cctaagtttt aaatatatca aaaattaatg ggaaactctt cgcgatttgt
    28261 gatgtctaac gggccatttc atgtaacaga acgtttccat acaccgctat ccatctaaat
    28321 ttaaatcact ttttcagaga actgcgtaag tattacgcat gttttccctg tcattcatcc
    28381 agattattcc taatcaccag actaatgatt ccatcaatcc tggcgcattt tagtcaaaac
    28441 gggggaaaat tttttcaaca aatgctcaac cagcattggg tatatccagt acactccacg
    28501 ctttacttaa gtctagatat ttgtgggaga aaggatgcct ggttcattac gtaaaatgcc
    28561 ggtctggtta ccaatagtca tattgctcgt tgccatggcg tctattcagg gtggagcctc
    28621 gttagctaag tcactttttc ctctggtggg cgcaccgggt gtcactgcgc tgcgtctggc
    28681 attaggaacg ctgatcctca tcgcgttctt taagccatgg cgactgcgct ttgccaaaga
    28741 gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt gggatgaatt atctttttta
    28801 tctttctatt cagacagtac cgctgggtat tgcggtggcg ctggagttca ccggaccact
    28861 ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc gtctgggttg tgctggcggt
    28921 tcttggtctg tggttcctgc taccgctggg gcaagacgtt tcccatgtcg atttaaccgg
    28981 ctgtgcgctg gcactggggg ccggggcttg ttgggctatt tacattttaa gtgggcaacg
    29041 cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt tcgttgattg cagcgttaat
    29101 tttcgtgcca attggagcgc ttcaggctgg tgaagcactc tggcactggt cggttattcc
    29161 attgggtctg gctgtcgcta ttctctcgac cgctctgcct tattcgctgg aaatgattgc
    29221 cctcacccgt ttgccaacac ggacatttgg tacgctgatg agcatggaac cggcgctggc
    29281 tgccgtttcc gggatgattt tcctcggaga aacactgaca cccatacagc tgctggcgct
    29341 cggcgctatc atcgccgctt caatggggtc tacgctgaca gtacgcaaag agagcaaaat
    29401 aaaagaatta gacattaatt aaatttacat ttctgcatgg ttatgcataa ccatgcagaa
    29461 tttctcgcta cttttcctct acaccgtctt tatatatcga attatgcaaa agcatattta
    29521 ttccgaaaat tcctggcgag cagataaata agaattgttc ttatcaatat atctaactca
    29581 ttgaatcttt attagttttg tttttcacgc ttgttaccac tattagtgtg ataggaacag
    29641 ccagaatagc ggaacacata gccggtgcta tacttagtct cgttaattac tgggacataa
    29701 catcaagagg atatgaaatt atgagtaccg ctaaattagt taaatcaaaa gcgaccaatc
    29761 tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa agcaacagta gagttgctga
    29821 atcgccaggt tatccagttt attgatcttt ctttgattac caaacaagcg cactggaaca
    29881 tgcgcggcgc taacttcatt gccgtacatg aaatgctgga tggcttccgc accgcactga
    29941 tcgatcatct ggataccatg gcagaacgtg cagtgcagct gggcggtgta gctctgggga
    30001 ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta cccgctggac atccacaacg
    30061 ttcaggatca cctgaaagaa ctggctgacc gttacgcaat cgtcgctaat gacgtacgca
    30121 aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat cctgaccgcc gcgtctcgcg
    30181 acctggataa attcctgtgg tttatcgagt ctaacatcga ataaatccat cgctgatggt
    30241 gcagaacttt agtacccgat aaaagcggct tcctgacagg aggccgtttt gttttgcagc
    30301 ccacctcaac gcacttattt agtgcatcca tctgctatct ccagctgatt aagtaaattt
    30361 tttgtatcca catcatcaca caatcgttac ataaagattg ttttttcatc aggttttacg
    30421 ctaaataatc actgtgttga gtgcacaatt ttagcgcacc agattggtgc cccagaatgg
    30481 tgcatcttca gggtattgcc ctataaatcg tgcatcacgt ttttgccgca tctcgaaaaa
    30541 tcaaggagtt gcaaaactgg cacgattttt tcatatatgt gaatgtcacg caggggatcg
    30601 tcccgtggat agaaaaaagg aaatgctatg aagtctgtat taaaagtttc actggctgca
    30661 ctgaccctgg cttttgcggt ttcttctcat gccgcggata aaaaattagt tgtcgcgacg
    30721 gataccgcct tcgttccgtt tgaatttaaa cagggcgata aatatgtggg ctttgacgtt
    30781 gatctgtggg ctgccatcgc taaagagctg aagctggatt acgaactgaa gccgatggat
    30841 ttcagtggga tcattccggc actgcaaacc aaaaacgtcg atctggcgct ggcgggcatt
    30901 accatcaccg acgagcgtaa aaaagcgatc gatttctctg acggctacta caaaagcggc
    30961 ctgttagtga tggtgaaagc taacaataac gatgtgaaaa gcgtgaaaga tctcgacggg
    31021 aaagtggttg ctgttaagag cggtactggc tccgttgatt acgcgaaagc aaacatcaaa
    31081 actaaagatc tgcgtcagtt cccgaacatc gataacgcct atatggaact gggcaccaac
    31141 cgcgcagacg ccgttctgca cgatacgcca aacattctgt acttcatcaa aaccgccggt
    31201 aacggtcagt tcaaagcggt aggtgactct ctggaagcgc agcaatacgg tattgcgttc
    31261 ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg cgttgaaaac cctgcgcgag
    31321 aacggaactt acaacgaaat ctacaaaaaa tggttcggta ctgaaccgaa ataataacgc
    31381 tacacctgta aagcgcactg gcagttccct ctcccctctg gggagaggat tagggtgagg
    31441 ggcgcaaacc cgctccgggg ccattaatta ccctgaattt gattatttac accacggtaa
    31501 caggaacaac atatgcagtt tgactggagt gccatctggc ctgccattcc gcttctgatt
    31561 gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc tggctggcgg tctggtaatc
    31621 ggattgctgg caggttttgc acgcaccttc ggtggttgga tagccaacca cgtcgcgctg
    31681 gtctttattg aggtgatccg cggcacacct atcgtcgtcc aggtgatgtt tatttatttc
    31741 gccctgccga tggcgtttaa cgacttacgc atcgacccat ttactgcggc ggtggtcacc
    31801 atcatgatca actccggcgc gtacattgcg gaaatcacgc gtggtgcggt gctgtctatt
    31861 cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt cacgttggga aaccattcgc
    31921 tacgtcattt taccgctggc actgcgtcgt atgctgccgc cgctgggtaa ccagtggatc
    31981 atcagcatta aagacacctc gctgtttatt gtgatcggcg tggcggaact gacccgtcag
    32041 gggcaagaaa ttattgccgg taacttccgc gcccttgaga tctggagcgc cgtggcggtg
    32101 ttctatctga ttattaccct ggtgctaagc tttattttgc gtcgtctgga aagaaggatg
    32161 aaaatcctgt gattgaattt aaaaacgtct ccaagcactt tggcccaacc caggtgcttc
    32221 acaatatcga tttgaacatt gcccagggcg aagtcgtggt gattatcggg ccgtccggtt
    32281 ccggtaaatc gaccctgctg cgctgcatca acaaactgga agaaatcacc tccggcgatc
    32341 tgattgtcga tggcctgaag gttaacgatc cgaaagttga cgagcgcctg attcgccagg
    32401 aagcaggtat ggtgttccag cagttttacc tcttcccgca tctgacggcg ctggaaaacg
    32461 tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga agaggcggaa aaactggcgc
    32521 gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca tcactaccct tccgaacttt
    32581 ctggtggtca acagcagcgt gtggcgattg cccgcgcgct ggcggtgaag ccgaaaatga
    32641 tgctgtttga tgaaccgact tccgctcttg acccggaact gcgccatgaa gtgctgaagg
    32701 ttatgcagga tctggctgaa gaagggatga cgatggtgat cgtgacccac gaaatcggtt
    32761 ttgccgagaa agtagcttcg cggctgatct ttatcgacaa aggccggatt gcggaagatg
    32821 gcgatccgca ggtattgatc aagaacccgc cgagccagcg cttgcaggaa tttttgcagc
    32881 acgtctctta acttctctgc ctccctttca cccgaaaggg aggcacacca gattcctctc
    32941 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
    33001 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
    33061 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
    33121 ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca ctggcggatg tgctggataa
    33181 cgacacctcg cgtaaagaat tgatcgacca gttgcgtact gtcgccgcga cgccccctgc
    33241 ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg tgctgcaaaa
    33301 ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg ggcaacttta
    33361 tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca gcaatgcgct
    33421 gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga ttcgcctgtg
    33481 cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg caaaaaaatc gtgagcgcag
    33541 caactggttg cagcttccgg cgatgattat cggggcgttt attatcgacc tgctgttact
    33601 ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag gcagtcgcac
    33661 catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat ttttcaaagc
    33721 cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca cgattcagga
    33781 cgagagcgcc cgttactgga gtcgtcgcct gagctggtta agcagtttga taggttatgg
    33841 cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag gtgcgctggc
    33901 gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct ttcgtaataa
    33961 aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggcct ttttcagcct
    34021 gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt ttatcgtgct
    34081 gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa tttatgatgg gtgcaacggt
    34141 gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt cccgctggct
    34201 ggcaaaaacc atcactctct cgccacatac tcagcgtaac tatccagagt tgcaaaaacg
    34261 gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg tctgcgtggc
    34321 ggtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc tgcaaaacgg
    34381 cgcggggcag aaaaccgtag atatcctgat ccgtatcgca cttattcttt ttttctcggc
    34441 ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg atattcatgg
    34501 ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta acgcgctggc
    34561 ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg tcaatatcgc
    34621 gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt cgcaaacgct
    34681 ggtgaaagat atcattaccg gggtgtttat tcagtttgaa aacggcatga acactggaga
    34741 tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc gctccgtggg
    34801 cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa ccacctttgc
    34861 taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc gccatgaaga
    34921 tgctgataaa gccaatcagg cactgaaaga tgcggtagcg gaattaatgg aaaacgaaga
    34981 aattcgcggg ctgattattg gtgaaccgaa ttttgccggg attgtcggct taagcaatac
    35041 cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga cggtacgctt
    35101 tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg cgccagtgca
    35161 gacttatcag gtgctgccta ctccgggcgc gaccccggct gaaccgttac cgccggggga
    35221 gccaacgctt taacgctggc gattgacaaa acggcggcgc tgctcgtcgt tcataaaggt
    35281 ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta ccaccttcac
    35341 cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac gagataccag
    35401 cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca tctttttgat
    35461 aaaggcgact tcaccacctt cacaccacaa ctcctgttgc tgaccaccaa agttcagtgc
    35521 atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc gcgctgcagc
    35581 ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt ttttatggat
    35641 gatgccgtta aaaattgccc cactctcttt ttgccgacgc agacgaatgg cgcggttaag
    35701 ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc tggtttcact
    35761 accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac agttcgcacc
    35821 aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt cagccagtaa
    35881 atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga gaaaaccgtc
    35941 ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat tgagcgcctt
    36001 caccgccagc ggattggcaa agtctacgct ttgctccccg gcgggtgtaa gcgtgaggaa
    36061 ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc tgtgatgacg
    36121 gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta agcagcggcg
    36181 taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat tccccaaaac
    36241 cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg catgtcatta
    36301 atttgtctta ttttgtaaaa atatatcgtt caaaaaataa gcacatctct gtcaattttt
    36361 tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt tgtatttatg
    36421 aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg agcggaatct
    36481 ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag ctacggcccg
    36541 ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat aaactggcag
    36601 aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc
    36661 agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc attcgcctct
    36721 ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata
    36781 aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gccaagtgat aatgcttatc
    36841 aaaattatta tcacttgcat gaacactatc acgggattaa cagtggcatc gcatccgcag
    36901 agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc tgcaaaatgg
    36961 aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt gccggtcttt
    37021 gtattggcat cacgcctgtg gctcaggcac tcgccgccga agggcaaact aacgcggatg
    37081 acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa tctgccgatc
    37141 cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt tctgaacaag
    37201 tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgcc cccggcg
//
LOCUS       DAZOZD010000015        36607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOZD010000015 DAZOZD010000000
VERSION     DAZOZD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.015"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUPB_000598"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUPB_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0757499.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUPB_000599"
     CDS             complement(280..873)
                     /locus_tag="ACHUPB_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0757500.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUPB_000600"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUPB_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0757501.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUPB_000601"
     CDS             1788..2183
                     /locus_tag="ACHUPB_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0757502.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUPB_000602"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUPB_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0757503.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUPB_000603"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUPB_000603"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0757504.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUPB_000604"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUPB_000604"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0757505.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUPB_000605"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUPB_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0757506.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUPB_000606"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUPB_000606"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0757507.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUPB_000607"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUPB_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0757508.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUPB_000608"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUPB_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0757509.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUPB_000609"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUPB_000609"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0757510.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUPB_000610"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUPB_000610"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0757511.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUPB_000611"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUPB_000611"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0757512.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUPB_000612"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUPB_000612"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0757513.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUPB_000613"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUPB_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR0757514.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUPB_000614"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUPB_000614"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0757515.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUPB_000615"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUPB_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0757516.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUPB_000616"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUPB_000616"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0757517.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUPB_000617"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUPB_000617"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0757518.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUPB_000618"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUPB_000618"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR0757519.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUPB_000619"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUPB_000619"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0757520.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUPB_000620"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUPB_000620"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0757521.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUPB_000621"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUPB_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR0757522.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUPB_000622"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUPB_000622"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0757523.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUPB_000623"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUPB_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0757524.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUPB_000624"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUPB_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0757525.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUPB_000625"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUPB_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0757526.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUPB_000626"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUPB_000626"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0757527.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUPB_000627"
     CDS             33227..34570
                     /locus_tag="ACHUPB_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0757528.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHUPB_000628"
     CDS             complement(34650..34856)
                     /locus_tag="ACHUPB_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0757529.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHUPB_000629"
     CDS             35181..35735
                     /locus_tag="ACHUPB_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0757530.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..>36607)
                     /gene="ytfI"
                     /locus_tag="ACHUPB_000630"
     CDS             complement(35825..>36607)
                     /gene="ytfI"
                     /locus_tag="ACHUPB_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0757531.1"
                     /translation="ISNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLIS
                     FSLKDNGAEYTMTLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQK
                     KQSDIPKRIKGYIHQAIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHE
                     KIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELA
                     EQVDAIVSANLAADSNITKEKICKKYKSTIEAFKKEQLALPV"
BASE COUNT         8857 a         9823 c         9115 g         8812 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaat
//
LOCUS       DAZOZD010000016        36331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOZD010000016 DAZOZD010000000
VERSION     DAZOZD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.016"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="ACHUPB_000631"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="ACHUPB_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR0757532.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /gene="yqfG"
                     /locus_tag="ACHUPB_000632"
     CDS             1867..1992
                     /gene="yqfG"
                     /locus_tag="ACHUPB_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0757533.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="ACHUPB_000633"
     CDS             complement(1989..2060)
                     /locus_tag="ACHUPB_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757534.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="ACHUPB_000634"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="ACHUPB_000634"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0757535.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="ACHUPB_000635"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="ACHUPB_000635"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0757536.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="ACHUPB_000636"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="ACHUPB_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0757537.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="ACHUPB_000637"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="ACHUPB_000637"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0757538.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="ACHUPB_000638"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="ACHUPB_000638"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0757539.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="ACHUPB_000639"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="ACHUPB_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0757540.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="ACHUPB_000640"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="ACHUPB_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0757541.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="ACHUPB_000641"
     CDS             complement(9465..9872)
                     /locus_tag="ACHUPB_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0757542.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="ACHUPB_000642"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="ACHUPB_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0757543.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="ACHUPB_000643"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="ACHUPB_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0757544.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="ACHUPB_000644"
     CDS             complement(11538..12197)
                     /locus_tag="ACHUPB_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0757545.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /gene="yqfB"
                     /locus_tag="ACHUPB_000645"
     CDS             complement(12361..12672)
                     /gene="yqfB"
                     /locus_tag="ACHUPB_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR0757546.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="ACHUPB_000646"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="ACHUPB_000646"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0757547.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="ACHUPB_000647"
     CDS             complement(14207..14950)
                     /locus_tag="ACHUPB_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0757548.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="ACHUPB_000648"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="ACHUPB_000648"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0757549.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="ACHUPB_000649"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="ACHUPB_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0757550.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="ACHUPB_000650"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="ACHUPB_000650"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0757551.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="ACHUPB_000651"
     CDS             20005..20088
                     /locus_tag="ACHUPB_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757552.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="ACHUPB_000652"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="ACHUPB_000652"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0757553.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="ACHUPB_000653"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="ACHUPB_000653"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0757554.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="ACHUPB_000654"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="ACHUPB_000654"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0757555.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="ACHUPB_000655"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="ACHUPB_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0757556.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="ACHUPB_000656"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="ACHUPB_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0757557.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="ACHUPB_000657"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUPB_000657"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="ACHUPB_000658"
     CDS             25234..25836
                     /locus_tag="ACHUPB_000658"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0757558.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="ACHUPB_000659"
     CDS             complement(25937..25996)
                     /locus_tag="ACHUPB_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0757559.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="ACHUPB_000660"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="ACHUPB_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0757560.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="ACHUPB_000661"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="ACHUPB_000661"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0757561.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="ACHUPB_000662"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="ACHUPB_000662"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0757562.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="ACHUPB_000663"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="ACHUPB_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0757563.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="ACHUPB_000664"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="ACHUPB_000664"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0757564.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="ACHUPB_000665"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="ACHUPB_000665"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0757565.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="ACHUPB_000666"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="ACHUPB_000666"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0757566.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="ACHUPB_000667"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="ACHUPB_000667"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR0757567.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36331)
                     /locus_tag="ACHUPB_000668"
     CDS             complement(35716..>36331)
                     /locus_tag="ACHUPB_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0757568.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9742 c         9170 g         8716 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DAZOZD010000017        34955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOZD010000017 DAZOZD010000000
VERSION     DAZOZD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.017"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUPB_000669"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUPB_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0757569.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUPB_000670"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUPB_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0757570.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUPB_000671"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUPB_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0757571.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUPB_000672"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUPB_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0757572.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUPB_000673"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUPB_000673"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0757573.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUPB_000674"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUPB_000674"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0757574.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUPB_000675"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUPB_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0757575.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUPB_000676"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUPB_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0757576.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUPB_000677"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUPB_000677"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0757577.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUPB_000678"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUPB_000678"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0757578.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHUPB_000679"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHUPB_000679"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0757579.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHUPB_000680"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHUPB_000680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0757580.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUPB_000681"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUPB_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0757581.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUPB_000682"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUPB_000682"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0757582.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUPB_000683"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUPB_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0757583.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUPB_000684"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUPB_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0757584.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHUPB_000685"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHUPB_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0757585.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUPB_000686"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUPB_000686"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0757586.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUPB_000687"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUPB_000687"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0757587.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUPB_000688"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUPB_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0757588.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUPB_000689"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUPB_000689"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0757589.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUPB_000690"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUPB_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0757590.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUPB_000691"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHUPB_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0757591.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUPB_000692"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHUPB_000692"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0757592.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHUPB_000693"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHUPB_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUPB_000694"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHUPB_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0757593.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHUPB_000695"
     CDS             complement(31717..33003)
                     /locus_tag="ACHUPB_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0757594.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHUPB_000696"
     CDS             complement(33054..33995)
                     /locus_tag="ACHUPB_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0757595.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHUPB_000697"
     CDS             complement(34010..34780)
                     /locus_tag="ACHUPB_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0757596.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         8339 a         9711 c         9164 g         7741 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcctg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgta
//
LOCUS       DAZOZD010000018        34337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOZD010000018 DAZOZD010000000
VERSION     DAZOZD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.018"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..268)
                     /locus_tag="ACHUPB_000698"
     CDS             complement(<1..268)
                     /locus_tag="ACHUPB_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0757597.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPV"
     gene            complement(428..640)
                     /gene="rpmE"
                     /locus_tag="ACHUPB_000699"
     CDS             complement(428..640)
                     /gene="rpmE"
                     /locus_tag="ACHUPB_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0757598.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            843..3041
                     /gene="priA"
                     /locus_tag="ACHUPB_000700"
     CDS             843..3041
                     /gene="priA"
                     /locus_tag="ACHUPB_000700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0757599.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3197..4222
                     /gene="cytR"
                     /locus_tag="ACHUPB_000701"
     CDS             3197..4222
                     /gene="cytR"
                     /locus_tag="ACHUPB_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0757600.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4314..5273
                     /gene="ftsN"
                     /locus_tag="ACHUPB_000702"
     CDS             4314..5273
                     /gene="ftsN"
                     /locus_tag="ACHUPB_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0757601.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5366..5896
                     /gene="hslV"
                     /locus_tag="ACHUPB_000703"
     CDS             5366..5896
                     /gene="hslV"
                     /locus_tag="ACHUPB_000703"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0757602.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5906..7237
                     /gene="hslU"
                     /locus_tag="ACHUPB_000704"
     CDS             5906..7237
                     /gene="hslU"
                     /locus_tag="ACHUPB_000704"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0757603.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7304..8230
                     /gene="menA"
                     /locus_tag="ACHUPB_000705"
     CDS             7304..8230
                     /gene="menA"
                     /locus_tag="ACHUPB_000705"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0757604.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8323..8808
                     /gene="rraA"
                     /locus_tag="ACHUPB_000706"
     CDS             8323..8808
                     /gene="rraA"
                     /locus_tag="ACHUPB_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0757605.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="ACHUPB_000707"
     CDS             complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="ACHUPB_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0757606.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9564..10409
                     /gene="glpF"
                     /locus_tag="ACHUPB_000708"
     CDS             9564..10409
                     /gene="glpF"
                     /locus_tag="ACHUPB_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR0757607.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10432..11943
                     /gene="glpK"
                     /locus_tag="ACHUPB_000709"
     CDS             10432..11943
                     /gene="glpK"
                     /locus_tag="ACHUPB_000709"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0757608.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12073..13083
                     /gene="glpX"
                     /locus_tag="ACHUPB_000710"
     CDS             12073..13083
                     /gene="glpX"
                     /locus_tag="ACHUPB_000710"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0757609.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13180..13926
                     /gene="fpr"
                     /locus_tag="ACHUPB_000711"
     CDS             13180..13926
                     /gene="fpr"
                     /locus_tag="ACHUPB_000711"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0757610.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="ACHUPB_000712"
     CDS             complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="ACHUPB_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0757611.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14386..14685)
                     /locus_tag="ACHUPB_000713"
     CDS             complement(14386..14685)
                     /locus_tag="ACHUPB_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0757612.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14897..15337)
                     /locus_tag="ACHUPB_000714"
     CDS             complement(14897..15337)
                     /locus_tag="ACHUPB_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0757613.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15438..16037
                     /locus_tag="ACHUPB_000715"
     CDS             15438..16037
                     /locus_tag="ACHUPB_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0757614.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16145..16912
                     /gene="tpiA"
                     /locus_tag="ACHUPB_000716"
     CDS             16145..16912
                     /gene="tpiA"
                     /locus_tag="ACHUPB_000716"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0757615.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="ACHUPB_000717"
     CDS             complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="ACHUPB_000717"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0757616.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="ACHUPB_000718"
     CDS             complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="ACHUPB_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0757617.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="ACHUPB_000719"
     CDS             complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="ACHUPB_000719"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0757618.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="ACHUPB_000720"
     CDS             complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="ACHUPB_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0757619.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="ACHUPB_000721"
     CDS             complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="ACHUPB_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0757620.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21981..22679
                     /gene="cpxR"
                     /locus_tag="ACHUPB_000722"
     CDS             21981..22679
                     /gene="cpxR"
                     /locus_tag="ACHUPB_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0757621.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22676..24049
                     /gene="cpxA"
                     /locus_tag="ACHUPB_000723"
     CDS             22676..24049
                     /gene="cpxA"
                     /locus_tag="ACHUPB_000723"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0757622.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="ACHUPB_000724"
     CDS             complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="ACHUPB_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0757623.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="ACHUPB_000725"
     CDS             complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="ACHUPB_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR0757624.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="ACHUPB_000726"
     CDS             complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="ACHUPB_000726"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0757625.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27126..28160
                     /gene="rhaT"
                     /locus_tag="ACHUPB_000727"
     CDS             27126..28160
                     /gene="rhaT"
                     /locus_tag="ACHUPB_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR0757626.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="ACHUPB_000728"
                     /pseudo
     CDS             complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="ACHUPB_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="ACHUPB_000729"
     CDS             complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="ACHUPB_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0757627.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30203..31672
                     /gene="rhaB"
                     /locus_tag="ACHUPB_000730"
     CDS             30203..31672
                     /gene="rhaB"
                     /locus_tag="ACHUPB_000730"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0757628.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31669..32928
                     /gene="rhaA"
                     /locus_tag="ACHUPB_000731"
     CDS             31669..32928
                     /gene="rhaA"
                     /locus_tag="ACHUPB_000731"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0757629.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33170..33994
                     /gene="rhaD"
                     /locus_tag="ACHUPB_000732"
     CDS             33170..33994
                     /gene="rhaD"
                     /locus_tag="ACHUPB_000732"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0757630.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34004..34318
                     /gene="rhaM"
                     /locus_tag="ACHUPB_000733"
     CDS             34004..34318
                     /gene="rhaM"
                     /locus_tag="ACHUPB_000733"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0757631.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8378 a         8952 c         8951 g         8056 t
ORIGIN      
        1 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac cttcgcaccg agcagcggat
      121 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
      181 caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag
      301 aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag
      361 aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa
      421 tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac
      481 cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga
      541 ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa gagcagctag
      601 cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg
      661 ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa
      721 tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt atgcaaactg
      781 atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg
      841 ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctgc
      901 tgccagaagg catggcggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc
      961 aggagcgcat cggggttgtg gtatcagtta gcgatgtcag tgagctgccg cttaacgagc
     1021 tgaaagcggt agttgaagtg ctggacgttg agccggtatt tactcactcc gtctggcgat
     1081 tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg tttcatgcct
     1141 tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg
     1201 ccactgaaca aggccaggcg gtggatctga acagcctgaa acgctcaccc aagcaacaac
     1261 aggcgctggc ggcgttacgg caagggaaaa tctggcgcga ccaggttgcc acgctcgaat
     1321 ttaatgatgc cgcgttgcag acgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa
     1381 caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg ttgcgattga
     1441 ataccgaaca ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact ttttctgcct
     1501 ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt gtactggaaa
     1561 acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc
     1621 aaactatcgc ccgttttcgt gaacggttta atgcgcccgt ggaagttctg cattccggtc
     1681 tgaacgacag cgagcgtctt tcggcgtggc tgaaagcgaa aaatggcgag gcggcgattg
     1741 tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg
     1801 acgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc
     1861 tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc gcaacgcccg
     1921 cgctggaaac gttgtgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacgcgcc
     1981 gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt cagaaggtgc
     2041 aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccaacaatc
     2101 aggttatcct ctttcttaac cgccgtggct ttgcgcctgc gctactgtgc cacgactgcg
     2161 gctggattgc cgaatgccca cgttgcgatc actactacac gctgcatcag gcgcaacagc
     2221 atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg
     2281 gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc
     2341 cgctgttccc tgatgtgccc atttctcgta tcgaccgcga taccaccagc cgcaaagggg
     2401 cgctggaaca gcaactggcg gaagtacatc gcggcggtgc gaggattttg atcggcacac
     2461 aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta ctggatgtgg
     2521 acggcgcgct gttttctgcc gattttcgct cggcagagcg tttcgctcag ctttacaccc
     2581 aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcacc
     2641 atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc tttgccgaac
     2701 aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat gtgattgtgc
     2761 gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg cgtaatctga
     2821 tcctctccag cccgctggca gacgacaaat tgtgggttct cggtccggtt ccggcactgg
     2881 cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct tcccgcgtgc
     2941 gcttgcaaca catcattaac ggtacgctgg cactcatcaa tacaataccg gattcccgta
     3001 aggtgaaatg ggtgctggat gttgatccga ttgagggtta aaccgctcac gatgcgaggc
     3061 ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc
     3121 gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc
     3181 caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt
     3241 tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga atcccgataa
     3301 agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg gttatttacc
     3361 gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc
     3421 ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa
     3481 tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt
     3541 tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc
     3601 gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt
     3661 cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg catttgatgc
     3721 agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga
     3781 gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat
     3841 tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc
     3901 gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc atagcgatgt
     3961 gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct
     4021 ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac
     4081 catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat
     4141 gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg
     4201 atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc
     4261 gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac
     4321 aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac
     4381 ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg
     4441 tgcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg
     4501 agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca gaagaacgct
     4561 ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt
     4621 ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg
     4681 aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt ccgtggaatg
     4741 aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag
     4801 cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga
     4861 cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta
     4921 ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc
     4981 cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa
     5041 aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga
     5101 cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc aacaatggct
     5161 ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc
     5221 ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc
     5281 aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct
     5341 gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca
     5401 tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt
     5461 gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc
     5521 ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct
     5581 ggtcaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc tgcgcaaact
     5641 tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga
     5701 cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc
     5761 tgcggcgcgc gcgctgttag aaaacactga acttagcgcc cgtgaaattg ctgaaaaggc
     5821 gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt
     5881 aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga
     5941 actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg
     6001 taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa
     6061 tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa
     6121 gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctatgt
     6181 cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg
     6241 cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct
     6301 cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc agcaggaacc
     6361 gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga
     6421 aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat
     6481 ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc agaagcaaaa
     6541 agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa
     6601 actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat
     6661 cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt
     6721 ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca ccgtttccac
     6781 caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat
     6841 tgcgaaaccg tctgacctga tcccggaact gcaaggtcgg ctgccaatcc gcgttgaact
     6901 gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac
     6961 cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg
     7021 tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc
     7081 tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga
     7141 tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt
     7201 ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat
     7261 tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat
     7321 tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc
     7381 tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc cgctggtcgc
     7441 cctgctggta ctaattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta
     7501 cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac gcggcatgca
     7561 aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat
     7621 ctgtctctcc gggctggcac tggttgcagt ggcatgccat acgctggccg attttgtcgg
     7681 tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg
     7741 tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg gctggttgag
     7801 cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc
     7861 gaccgcgtgt ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa
     7921 tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg
     7981 tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt
     8041 ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac tggtgaagca
     8101 agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc tggaacgtac
     8161 tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc taagccagtg
     8221 ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag cctacatagc
     8281 gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc
     8341 gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt
     8401 ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg
     8461 ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt
     8521 ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa
     8581 tgggaaggtc tggttattta cggcgcggtg cgtcaggtag atgacctaga agagttggat
     8641 atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa
     8701 agcgatgtcc gcgtcaattt tggcggtgta accttcttct ccggcgacca tctttatgcc
     8761 gacaataccg ggattattct ttcagaagat ccgctggata ttgaatgata agaaaggcac
     8821 cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa ttacgcgaaa
     8881 gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac gttcctgcca
     8941 gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc gctgatgctg
     9001 ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt cgatttccat
     9061 ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt tctcaaacac
     9121 ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata aaaaatcctc
     9181 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
     9241 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
     9301 catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg
     9361 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaattgcgc gataacgctc
     9421 attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
     9481 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
     9541 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
     9601 ctgaattcct cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt gcagcactaa
     9661 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
     9721 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca
     9781 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
     9841 aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt
     9901 tcgacttcga gcagactcat cacattgttc gcggcagtgt tgaaagtgtt gatctggcag
     9961 gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga
    10021 tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg
    10081 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    10141 ctatgggccc attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    10201 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    10261 tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    10321 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gagaccacaa
    10381 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    10441 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    10501 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    10561 ggctgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    10621 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    10681 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    10741 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    10801 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    10861 tggatcctcg accatgtgga aggctctcgc gagcgtgcgc gtcgtggtga attgctgttt
    10921 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    10981 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    11041 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    11101 gtgtacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    11161 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    11221 aacacctatg gcactggctg ctttatgctg atgaacaccg gcgagaaagc ggtgaaatca
    11281 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgctg
    11341 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    11401 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    11461 tatgtggttc cggcctttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    11521 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    11581 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    11641 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    11701 tccgatattc tcggcacccg cgttgagcgc ccggaagttc gcgaagtgac tgcattgggc
    11761 gcggcctatc ttgctggtct ggcggtcggt ttctggcaga acctcgacga gctgcaagag
    11821 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    11881 tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgtaatg
    11941 taaatgccgg atgaagcgtt tatgccgcat ccggtagtcc cgaaacgtgc gggggcaacc
    12001 ccgcacacat caataatccc tcccttcccc tgtgctacac ttcgcgccat tccttactgc
    12061 ttagagtttg ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg
    12121 gcgctggctg gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg
    12181 gtaaacgcca tgcgtattat gctcaaccag gtcaatattg acggcaccat cgtcattggt
    12241 gaaggtgaaa tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc
    12301 ggcgacgcgg tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc
    12361 caggctaacg cgctggcggt gctggcagta ggcgataaag gctgcttcct caatgcgccg
    12421 gatatgtata tggagaagct gatcgtcgga ccgggagcca aaggcaccat tgatttgaac
    12481 ctgccgctgg cggataacct gcgcaatgtt gcggcggcgc tcggcaaacc gttgagcgaa
    12541 ctgacggtaa caattctggc taaaccacgc cacgatgccg ttatcgctga aatgcaacaa
    12601 ctcggcgtac gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt
    12661 atgccagaca gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt
    12721 tctgcggcgg tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat
    12781 gacgtcaaag gcgacaacga agagaatcgt cgcattggcg agcaggagct ggcacgctgc
    12841 aaagcgatgg gcatcgaagc cggtaaagta ttgcgcctgg gcgatatggc gcgcagcgat
    12901 aacgtcatct tctctgccac cggtattacc aaaggcgatc tgctggaagg cattagccgc
    12961 aaaggcaata tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc
    13021 cgcattcagt ctatccacta tctggatcgc aaggacccgg aaatgcaggt gcacatcctc
    13081 tgattgattt gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag
    13141 aacgaagata aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc
    13201 aaagtcacta aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc
    13261 gtgcttccgt ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc
    13321 gtccagcgcg cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg
    13381 gtcaccgtcc ccgatggcaa attaagccca cgactggcgg cactgaaacc aggcgatgaa
    13441 gtgcaggtgg ttagcgaagc agctggcttc tttgttctgg aggaagtacc agattgcgaa
    13501 acgctatgga tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta
    13561 ggcaaagatt tagatcgctt caaaaatctg gtcctggttc acgccgcacg ttatgccgcc
    13621 gacttaagct atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc
    13681 attcagacgg tggtcagtcg ggaaacggca gcggggtcgc tcaccggacg gataccggca
    13741 ttaattgaaa gtggggaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc
    13801 catgtgatgc tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag
    13861 acccggcaga tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac
    13921 tggtaagcgg ttacttatcg ataaacggca cgatgagcaa atccgcactc atcttattga
    13981 tcatcccgcg atatgccggc atcaaacggt tgataaatga gtggtgatga ccacagacaa
    14041 ggaggtcgca ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt
    14101 caatacgcag ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat
    14161 ccgacttatt cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat
    14221 acaactcgct taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg
    14281 cgagctccag ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc
    14341 caatgtgttt ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg
    14401 cagtgataac tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag
    14461 ctcaacttgc ccctccagat ccttaatctt ataggccacg ctggcccctt catctgcaga
    14521 ggatgcagcg gcggcaagct ctttcagttt ggtcataaaa gactctgctt cttcgcgggt
    14581 ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc
    14641 aatcgcacat cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat
    14701 attcgcctct ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa
    14761 gtaatgatct tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg
    14821 attttccggg tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta
    14881 agagatatat taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctgag
    14941 tatctttacc atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca
    15001 tcatcaccag aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta
    15061 aaatcgccca gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc
    15121 cggaacgccc gcgatcatgc aagcgcttaa cagttactgc cgctgttggc caaagcaagc
    15181 acacaaggca aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa
    15241 gcaccagcat gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac
    15301 gccctttaaa tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt
    15361 ttccaggatt ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat
    15421 aacaaaaggt actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat
    15481 taaccgttac aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc
    15541 tgctggctgg agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca
    15601 gccagaatcc tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag
    15661 ccaatctcac tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg
    15721 agcgcggtac cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag
    15781 agcaaaaagc agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca
    15841 ccccattaat gaccaaaaca caaagccaga aaaaactgca gtcgctacta acggcgggga
    15901 aaaacaaacg ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaaca
    15961 gcgaaaaggg gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg
    16021 aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc
    16081 acagacctgc tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat
    16141 taaaatgcga catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt
    16201 tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc
    16261 aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat
    16321 catgctgggt gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc
    16381 tgccgctatg ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg
    16441 tacttaccac aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca
    16501 gggcctgact ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac
    16561 tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt
    16621 cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac
    16681 tccggctcag gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc
    16741 taacatcgct gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc
    16801 agaactgttt gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc
    16861 tgacgctttc gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca
    16921 ggtgccggat ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct
    16981 cacactgatg atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt
    17041 gtgtcgccag caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc
    17101 ccatatgttc ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta
    17161 ccagttcgct ttccgttacc cgacgcgcca ggtattcatg cccacgcaaa ccacctggca
    17221 gtggcaacca gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag
    17281 gacgaataca agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga
    17341 tcgccaaaga aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac
    17401 gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt
    17461 cagtaccgtt aatacgatac gttggcatca acagatattg cagtggacca ttaagatctt
    17521 ttaaaaccac gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt
    17581 gctgctgatt gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac
    17641 cggttaattt ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa
    17701 aaagaagacc cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac
    17761 gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc accgggtttg ataattatcc
    17821 tggggaaatc agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt
    17881 tgcgctttcg tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac
    17941 gcattttcgt actttttcgc tacttcagcg tcgcgcggac ggtagtagtt tttcgcggca
    18001 atttcctgac cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg
    18061 ccttttttct cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca
    18121 ctcggcgtga cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg
    18181 ttttcccagg cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg
    18241 cgcgcgccag aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct
    18301 tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc aggcagctag gtagttccag
    18361 cgtgcgccac cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga
    18421 tcgttccagt catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa
    18481 gtgtacggtg cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccacgt
    18541 tccgcaatcg cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg
    18601 ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta
    18661 tcaccagttt gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg
    18721 cgcgttggat catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc
    18781 gccagcaaaa atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg
    18841 ttgtgatgag caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg
    18901 ctataacttg cgggtatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg
    18961 ctgacaaagt gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa
    19021 agtttgcaaa ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg
    19081 catttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta
    19141 atacagtttt ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat
    19201 agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc
    19261 gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc
    19321 gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg
    19381 accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat
    19441 cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc
    19501 ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc
    19561 agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg
    19621 agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa
    19681 gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat
    19741 gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat
    19801 aaccaccagc gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt
    19861 ctcgtcgcgg aattccggga agcgcgcaga accgaggaac gtaccgccac ggttgatcat
    19921 gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc
    19981 gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat
    20041 tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat
    20101 gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat
    20161 cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag
    20221 gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga
    20281 ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat
    20341 aattacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat
    20401 atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt
    20461 cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac
    20521 gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac
    20581 cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa
    20641 cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc
    20701 gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt
    20761 ccggcgcacc acccaccgct gaaacgagac aaggataatc gtacaaatta gcgccacaat
    20821 tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatacca gatgttgaat
    20881 acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc
    20941 gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata
    21001 tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc
    21061 ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcag
    21121 cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt
    21181 cataaatact cccactatca actgacgcta gtataacgga agcaaatcat ctgcaatgca
    21241 ttaagcagca ggcaaattga ggataaaaaa aaccccacat catgtggggg aagacaggga
    21301 tggtgtctat ggcaaggaaa acagggttta ctactgggaa cgtgagttgc tactactcaa
    21361 tagcttcaac gatgaacttt tttgccattg cgtcacgtca cgcaactgct ccattcgttg
    21421 ttgatgtttc tcgtttaaaa ccgcttgctg ctccggcgtt aacaggcgat acatttggtt
    21481 gcggactttt gccatctcaa cctgacgagc aatttgctca ttcgccattt tttctgcctg
    21541 tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca aggcgatgca ttgtctccag
    21601 ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg
    21661 ctgacgctga tgttcggtta aacttatgcc gtcgaacata tggctctgcg tactgcgctg
    21721 cgtaagttct tcacccggat gccagttatc gcctgaaccg acttcagcag cgtggcttaa
    21781 tgaactgact gccagcgttg aggccatgac ggcagcggta actatgcgca tcatttgctc
    21841 ccaaaatctt tctgtcgcga ttcaacgata gagagtttac gattcaggct gcaaacatgc
    21901 gtcagggggt gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct
    21961 gcctcggagg tatttaaaca atgaataaaa tcctgttagt tgatgatgac cgagagctga
    22021 cttccctatt aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg
    22081 gggaacaggc gcttgatctt ctggacgaca gcattgattt acttttgctt gacgtaatga
    22141 tgccgaagaa aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg
    22201 tcattatgtt gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg
    22261 cagatgacta tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga
    22321 tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa cgacaacggt tcaccgacac
    22381 tggaagttga tgccttagtg ctgaatccag gccgtcagga agccagcttc gacgggcaaa
    22441 cgctggagtt aaccggtact gagtttaccc tgctctattt gctggcacag catctgggtc
    22501 aggtggtttc ccgtgaacat ttaagccagg aagtgttggg caaacgcctg acgcctttcg
    22561 accgcgctat tgatatgcac atttccaacc tgcgtcgtaa actgccggat cgtaaagatg
    22621 gtcacccgtg gtttaaaacc ttgcgtggtc gcggctatct gatggtttct gcttcatgat
    22681 aggcagctta accgcgcgca tcttcgccat cttctggctg acgctggcgc tggtgttgat
    22741 gttggttttg atgttaccca agctcgattc acgccagatg accgagcttc tggatagcga
    22801 acagcgtcag gggctgatga ttgagcagca tgtcgaagcg gagctggcga acgatccgcc
    22861 caacgattta atgtggtggc ggcgtctgtt ccgggcgatt gataagtggg caccgccagg
    22921 acagcgtttg ttattggtga ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat
    22981 gcagatcatt cgtaacttta ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa
    23041 gtatggccgc gtggaactgg tcggtccgtt ctccgtgcgt gatggcgaag ataattacca
    23101 actttatctg attcgtccgg ccagcagttc tcaatccgat ttcattaact tactgtttga
    23161 ccgcccgcta ttactgctga ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt
    23221 ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca
    23281 gggaaactta cgccagcacc cggaactgga agcggggcca caggaattcc ttgccgcagg
    23341 tgccagtttt aaccagatgg tcaccgcgct ggagcgcatg atgacctctc agcagcgtct
    23401 gctttctgat atctctcacg agctgcgcac cccgctgacg cgtctgcaac tgggtacggc
    23461 gttactgcgc cgtcgtagcg gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca
    23521 acgtctggac agcatgatca acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc
    23581 gctggttagc gaaaccatca aagccaacca gttgtggagt gaagtgctgg ataacgcggc
    23641 gttcgaagcc gagcaaatgg gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc
    23701 gctgtacggc aatccgaacg ccctggaaag tgcgctggaa aacattgttc gtaatgctct
    23761 gcgttattcc catacgaaga ttgaagtggg ctttgcggta gataaagacg gtatcaccat
    23821 tacggtggac gacgatggtc ctggcgttag cccggaagat cgcgaacaga ttttccgtcc
    23881 gttctatcgg accgatgaag cacgcgatcg tgaatctggc ggtacaggat tggggctggc
    23941 gattgttgaa accgccattc agcagcatcg tggctgggtg aaggcagaag acagcccgct
    24001 gggcggttta cggctggtga tttggttgcc gctgtataag cggagttaaa ctccgcattt
    24061 gtaggcagga taaggcgttt acgccgcatc cggcatttga gcaggatgcc tgatgcgacg
    24121 ctgatagcgt cttatcaggc ctacactccg ggggttattt tccccacaac cggcgggaaa
    24181 aatcttcgat cttgccgctc agtcggcgct tttgcatcgt ccgcgtccag cttttcgata
    24241 acccggcggc ggagagtaaa cggtgatact gatcgtcatc aaacggcata tgccatgcga
    24301 tggcggcagc ctcctgcacg gtcacatcgc tgacacggga aaccaactcc agcggcgcgt
    24361 ccgcagacac tttccctggc gctatcacgc tatacaacca ccccacctta cctgtgtttt
    24421 gcatcaactg cgctatatcg ctgatatcaa aatggtaatt gagtttatag caaggcgagc
    24481 gcggctggct gacctgaatt aatgcctctc cccagcggaa aatatcgccc atatgaacat
    24541 tactttccgt gagaccgtcg gttgagagat tttcgccaaa cgcaggcgca acaaacaact
    24601 ccgcctgttc cggaaattcc cgcacccagt agagatagtg ctcacgagga taatgacaca
    24661 gcgctctgtc tggcccgccg tgaacctttt tctccgcctg ctcgtcacct tccagcccca
    24721 gctccgtcag catcaactca ccatcaacct ggattttggc aattgcgctg ggtttgcctt
    24781 cgggataagc ctgaatcttg cctgtgtata catcaaccgg atatcgcatc tgttccctct
    24841 ccacgagaaa tttccccatt agaccatatt tattttatgt caggcgtgct gttttcgcgg
    24901 taagtatcca ggcaggaact caatagcaaa ccggacgaac gttagttcat ccggctgtgg
    24961 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
    25021 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
    25081 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
    25141 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
    25201 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
    25261 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
    25321 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcactgc tttgctgaaa
    25381 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
    25441 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
    25501 gtcatgagcg gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta
    25561 ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc
    25621 agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg
    25681 cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg
    25741 gatttacgca gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac
    25801 cacaccgcca gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat
    25861 ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc
    25921 atcccccccg ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat
    25981 atttttaagg gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat
    26041 agacattcat ttaatcaata aaagtgatca aaaccacatc aattgaaacg ctgttttatt
    26101 tttatcggat cattgttttt attttgaaat tgatcacaaa aaaaccaccg ccgttggcga
    26161 tggttcattt tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga
    26221 ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc
    26281 tttaatgtag tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc
    26341 caggcccaga atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc
    26401 ctggttagca gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc
    26461 ggaaccaaag cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc
    26521 gaagtcacgt tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc
    26581 tttccagaac aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt
    26641 gtctgctggc agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc
    26701 tggcaggctt tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg
    26761 gtggatttcc atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag
    26821 ggatggcagg gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc
    26881 cgcgtaagca gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc
    26941 gtacttttcg taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc
    27001 acttaatgcc gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga
    27061 cagcacgatt tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata
    27121 agaagatgag taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg
    27181 cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt
    27241 cagtcggtgg gattgtttcg tggattattc tgccgtggac catcagcgcc ctgttactac
    27301 cggatttctg ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt
    27361 tcggcgctat gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca
    27421 tgtcgatggg aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc
    27481 caattatcaa cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttgc
    27541 tcggcgttct ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag
    27601 agcgcaagat gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg
    27661 tgatgtgcgg cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc
    27721 atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt cgctctgcca agctatgttg
    27781 tcatcatggg cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag
    27841 tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg
    27901 tgttactctc ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctggg
    27961 gccacgcccg cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct
    28021 atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc
    28081 gtccggtaac ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg
    28141 gcatcggcat ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca
    28201 tcccggtttc ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg
    28261 tcgaaatatc actgattaac aggcggctat gctggagaag atattgcgca tgacacactc
    28321 tgacctgtcg cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca
    28381 aaacgcgctc actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca
    28441 ggctagccgc caaccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca
    28501 acgaatcact ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact
    28561 cagccatttc gttagcaaac gacacatgct gactactttc atgctcaagc tgaccgataa
    28621 cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg
    28681 cgctaaatcc cggaatcgct ccctgtcagt caagattcag cttcagccgc tccgggcaat
    28741 aaataatatt ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat
    28801 gaatataaaa gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac
    28861 cattaccgcg ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat
    28921 cttgcggata acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt
    28981 tgagaagttt taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat
    29041 tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt
    29101 ccctggcgaa tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg
    29161 ttactgtcgc tgaatccaca gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt
    29221 agcagatgtc gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc
    29281 gtttgctgct taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg
    29341 gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga
    29401 cgtgatgcgc tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat
    29461 tgcataaaca agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt
    29521 tccatctgtg caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga
    29581 gacggatact gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga
    29641 aatcgatccg gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac
    29701 agatgccgat catgagcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc
    29761 ccattaaaca catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga
    29821 tgatgttcag gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacca
    29881 gacggaaaaa aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac
    29941 ggcgaaatag taatcacgag gtcaggttct taccttaaat tttcgacgga aaaccacgta
    30001 aaaaacgtcg atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat
    30061 caccacaatg aagctaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
    30121 ccattttcct gtcagtaacg agaaggtcac gaattcaggc gctttttaga ctggtcgtaa
    30181 tgaaattcag caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc
    30241 atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg
    30301 cgaaatccat cgttttaaca atggcctgca tagccagaac ggttatgtca cctggaatgt
    30361 ggatagcctg gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg
    30421 tatcgatagc attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg
    30481 tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc tccaatggcc taatggcgca
    30541 ggcacagcaa caactcggca aacgcgatat ttatcaacgt agcggcatcc agtttctgcc
    30601 cttcaatacg ctttatcaac tgcgtgcgct gacggagcaa caacctgaac ttattccaca
    30661 cattgctcac gctctgctga tgccggatta cttcagctat cgcctgaccg gcaagatgaa
    30721 ctgggagtac accaacgcca cgaccacgca actggtcaat atcaatagcg acgactggga
    30781 cgagtcgcta ctggcgtgga gcggggctaa caaagcctgg tttggtcgcc cgacgcatcc
    30841 gggtaatgtc attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt
    30901 tgccagccat gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgctgc
    30961 ttatctctct tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa
    31021 tgacacggcg ctggcagcca acataaccaa tgaaggcggg gcggaaggtc gctatcgggt
    31081 gctgaaaaat attatgggct tatggctgct tcagcgagtg cttcaggagc ggcaaatcaa
    31141 cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa
    31201 tcccaatgac gatcgcttta ttaatcctga ggcgatgtgc agcgaaattc aggctgcgtg
    31261 tcgggaaatg gcgcaaccgc tcccggaaag tgatgctgaa ctggcgcgct gcattttcga
    31321 cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctac gcggtgaaga
    31381 tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg
    31441 tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa
    31501 tatcggcatc cagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt
    31561 cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca
    31621 ctatgtggcg cagcttcact cgacacgaca gacaaaggag ctttgcgcat gaccactcaa
    31681 ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag
    31741 gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat
    31801 gtttccggtt ttgaaaaccc ggaaggttcg ctgaccggtg ggattcaggc tattggtaat
    31861 tatccgggca aagcgcgtaa tgccagtgag ctacgagccg atctggaaca ggctatgcgg
    31921 ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc agacacgcca
    31981 gtctcgcgcg accagatcaa acctgagcac ttcaaaaact gggttgaatg ggcgaaagcc
    32041 aatcagctcg gtctggattt taacccctcc tgtttctcgc atccgctaag cgccgatggc
    32101 tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc
    32161 agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc
    32221 tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg
    32281 ctggcagcac tggatgaggt gatcagcgag aagctggatc cggcgcacca tatcgacgcc
    32341 gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt
    32401 tacatggggt atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcatttccac
    32461 ccgactgaag tgatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg
    32521 ctgcacgtca gccgtccggt tcgctgggac agcgatcacg tggtgctgct ggatgatgaa
    32581 acccaggcga ttgccagtga gattgttcgt cacgatctgt tcgaccgggt gcatatcggc
    32641 ctcgacttct ttgatgcctc tatcaaccgc attgccgcgt gggtcattgg tacacgcaat
    32701 atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg caagctggaa
    32761 gcggcgggcg attacactgc gcgtctggcg ctactggaag aacagaaatc gttgccgtgg
    32821 caggcggtct gggaaatgta ttgccaacgt cacgatacgc cagcaggtag cgaatggctg
    32881 gagagcgtgc gggcttatga gaaagcgatt ttgagccagc gtgggtaaac actgccggat
    32941 gcggcgcgag cgccttatcc ggcctacagg tcgaatgacc atgcgtgctg ccaccctcac
    33001 cccggccctc tccctgaaag ggcgagggag aaaaacatag cctcgtgcgg caacggagct
    33061 gaacaccgct ccggacagtc ccctcgcccc tacggggaga gggttagggt gaggggaaca
    33121 agccgcacca tgtctaaaca aggcaaccta atttacagga acacagaaca tgcaaaacat
    33181 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg
    33241 ctgggacgag cgcaatggtg gtaacctgac gctgcgcctg gatgacgccg atatcgcgcc
    33301 ttatcacggc aactttcatg cacaaccgcg ctatattccc ctcagccaac ccatgccttt
    33361 actggcaaat acgccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
    33421 tgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gacggcgcgg gctaccatat
    33481 tctctggggg ttaaccaacg aagccgttcc aacttccgaa cttccggctc acttcctttc
    33541 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
    33601 caccaacctg atcgccctca cctatgtact tgaaaacgac accgcggtct ttactcgcca
    33661 actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc
    33721 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
    33781 ttcgctggtg ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac
    33841 attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
    33901 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
    33961 cgttacacca ctagctagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
    34021 tgtcatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg
    34081 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    34141 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc
    34201 ggtagccagc actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
    34261 taacccggat aacagcccgg tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt
    34321 gcccaatgtg ccagaga
//
LOCUS       DAZOZD010000019        33814 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOZD010000019 DAZOZD010000000
VERSION     DAZOZD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.019"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(53..187)
                     /locus_tag="ACHUPB_000734"
     CDS             complement(53..187)
                     /locus_tag="ACHUPB_000734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757632.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            263..1153
                     /gene="metF"
                     /locus_tag="ACHUPB_000735"
     CDS             263..1153
                     /gene="metF"
                     /locus_tag="ACHUPB_000735"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0757633.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            1482..3662
                     /gene="katG"
                     /locus_tag="ACHUPB_000736"
     CDS             1482..3662
                     /gene="katG"
                     /locus_tag="ACHUPB_000736"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0757634.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            3756..4661
                     /gene="yijE"
                     /locus_tag="ACHUPB_000737"
     CDS             3756..4661
                     /gene="yijE"
                     /locus_tag="ACHUPB_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0757635.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(4688..5305)
                     /locus_tag="ACHUPB_000738"
     CDS             complement(4688..5305)
                     /locus_tag="ACHUPB_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR0757636.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(5579..6682)
                     /gene="gldA"
                     /locus_tag="ACHUPB_000739"
     CDS             complement(5579..6682)
                     /gene="gldA"
                     /locus_tag="ACHUPB_000739"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR0757637.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(6693..7355)
                     /gene="fsa"
                     /locus_tag="ACHUPB_000740"
     CDS             complement(6693..7355)
                     /gene="fsa"
                     /locus_tag="ACHUPB_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0757638.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(7367..9868)
                     /gene="ptsP"
                     /locus_tag="ACHUPB_000741"
     CDS             complement(7367..9868)
                     /gene="ptsP"
                     /locus_tag="ACHUPB_000741"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0757639.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            10177..11256
                     /locus_tag="ACHUPB_000742"
     CDS             10177..11256
                     /locus_tag="ACHUPB_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0757640.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            11271..11591
                     /locus_tag="ACHUPB_000743"
                     /pseudo
     CDS             11271..11591
                     /locus_tag="ACHUPB_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            11642..13938
                     /locus_tag="ACHUPB_000744"
                     /pseudo
     CDS             11642..13938
                     /locus_tag="ACHUPB_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            13904..14782
                     /locus_tag="ACHUPB_000745"
     CDS             13904..14782
                     /locus_tag="ACHUPB_000745"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0757641.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            14784..15125
                     /locus_tag="ACHUPB_000746"
     CDS             14784..15125
                     /locus_tag="ACHUPB_000746"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0757642.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(15112..15963)
                     /locus_tag="ACHUPB_000747"
     CDS             complement(15112..15963)
                     /locus_tag="ACHUPB_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0757643.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(16178..17911)
                     /locus_tag="ACHUPB_000748"
     CDS             complement(16178..17911)
                     /locus_tag="ACHUPB_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0757644.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(18094..20745)
                     /gene="ppc"
                     /locus_tag="ACHUPB_000749"
     CDS             complement(18094..20745)
                     /gene="ppc"
                     /locus_tag="ACHUPB_000749"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0757645.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(21097..22248)
                     /gene="argE"
                     /locus_tag="ACHUPB_000750"
     CDS             complement(21097..22248)
                     /gene="argE"
                     /locus_tag="ACHUPB_000750"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0757646.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            22402..23406
                     /gene="argC"
                     /locus_tag="ACHUPB_000751"
     CDS             22402..23406
                     /gene="argC"
                     /locus_tag="ACHUPB_000751"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0757647.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            23414..24190
                     /gene="argB"
                     /locus_tag="ACHUPB_000752"
     CDS             23414..24190
                     /gene="argB"
                     /locus_tag="ACHUPB_000752"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0757648.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            24251..25624
                     /gene="argH"
                     /locus_tag="ACHUPB_000753"
     CDS             24251..25624
                     /gene="argH"
                     /locus_tag="ACHUPB_000753"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0757649.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            25891..26808
                     /gene="oxyR"
                     /locus_tag="ACHUPB_000754"
     CDS             25891..26808
                     /gene="oxyR"
                     /locus_tag="ACHUPB_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0757650.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(26791..28191)
                     /gene="sthA"
                     /locus_tag="ACHUPB_000755"
     CDS             complement(26791..28191)
                     /gene="sthA"
                     /locus_tag="ACHUPB_000755"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0757651.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            28512..29162
                     /gene="fabR"
                     /locus_tag="ACHUPB_000756"
     CDS             28512..29162
                     /gene="fabR"
                     /locus_tag="ACHUPB_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0757652.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            29162..29521
                     /locus_tag="ACHUPB_000757"
     CDS             29162..29521
                     /locus_tag="ACHUPB_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0757653.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(29561..30661)
                     /gene="trmA"
                     /locus_tag="ACHUPB_000758"
     CDS             complement(29561..30661)
                     /gene="trmA"
                     /locus_tag="ACHUPB_000758"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0757654.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      30775..30965
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            31030..32874
                     /gene="btuB"
                     /locus_tag="ACHUPB_000759"
     CDS             31030..32874
                     /gene="btuB"
                     /locus_tag="ACHUPB_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0757655.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            32819..33676
                     /gene="murI"
                     /locus_tag="ACHUPB_000760"
     CDS             32819..33676
                     /gene="murI"
                     /locus_tag="ACHUPB_000760"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0757656.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         7730 a         8495 c         9305 g         8284 t
ORIGIN      
        1 ggattaagcg tttacgacga atccggcaag aagcaataag tacatggtta gtttatattt
       61 gcagtccggt ttgctttgca taccggattt tctttttctt accatcctga agttttttca
      121 tcttccctga tttttcctca ccatcattgg tcatttttcg gttgacgccc ttcggctttt
      181 ccttcatctt tacatctgga cgtctaaacg gatagatgtg cacaacacaa catataacta
      241 caagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg atgccctgaa
      301 tcagagcctg gcagaagtcc aggggcagat taacgtttcg ttcgagtttt tcccgccgcg
      361 taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca gcctgaaacc
      421 gaagtttgta tcggtgacct atggcgcgaa ctccggcgag cgcgaccgta cgcacagcat
      481 tattaaaggc attaaagatc gcactggtct ggaagcggca ccgcatctta cttgcattga
      541 tgcgacgccc gacgagttgc gcaccattgc acgcgactac tggaataacg gtattcgtca
      601 tatcgtggcg ctgcgtggcg atctgccgcc gggaagtggt aagccagaaa tgtatgcttc
      661 tgacctggtg acgctattaa aagaagtggc agatttcgat atctccgtgg cggcgtatcc
      721 ggaagttcac ccggaagcaa aaagcgctca ggcggatttg cttaatctga aacgcaaagt
      781 cgatgccgga gccaaccgcg cgattactca gttcttcttc gatgtcgaaa gctacctgcg
      841 ttttcgtgac cgctgtgtat cggcgggcat tgatgtggaa attattccgg gaattttgcc
      901 ggtatctaac tttaaacagg cgaagaaatt tgccgatatg accaacgtgc gtattccggc
      961 gtggatggcg caaatgttcg acggcctgga tgatgatgcc gaaacccgca aactggtggg
     1021 cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga aagatttcca
     1081 cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacgc tgggtgttcg
     1141 acctggttta taaatagtgt ggcttttgtg aaaatcacac agtgatcaca aattttaaac
     1201 agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttgtcag ccttgttttc
     1261 tccctcatta cttgaaggat atgaagctaa aacccttttt tataaagcat ttgtccgaat
     1321 tcggacataa tcaaaaaagc ttaattacga tcaatttgac ctacatctct ttaaccaaca
     1381 atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt ctctctaacg
     1441 ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg tcagacgata
     1501 tccataacac gacagccact ggcaaatgcc cgttccatca gggcggtcac gaccagagcg
     1561 cgggggcggg cacaaccact cgcgactggt ggccaaatca actccgtgtt gacctgttaa
     1621 accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc aaagaattca
     1681 gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca gaatctcagc
     1741 cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg gcctggcacg
     1801 gcgcaggtac ttaccgttca atcgatggac gcggcggcgc gggtcgtggt cagcaacgtt
     1861 ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt cgcctgttgt
     1921 ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt atcctcgcag
     1981 gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc ggtcgtgaag
     2041 acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg ctgactcacc
     2101 gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc ctgatttacg
     2161 ttaacccgga aggcccggat cacagcggcg aaccgctttc tgcggcagca gctatccgcg
     2221 cgaccttcgg caacatgggc atgaacgacg aagaaaccgt ggcgctgatt gcgggtggtc
     2281 atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct gatccagaag
     2341 ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc ggcgttggcg
     2401 cagatgccat tacctctggt ctggaagttg tctggaccca gacgccgacc cagtggagca
     2461 actatttctt cgaaaacctg ttcaaatatg agtgggtaca gacccgcagt ccggctggcg
     2521 caatccagtt cgaagcggta gacgcaccgg aaattatccc ggatccattc gatccgtcga
     2581 agaaacgtaa accgacgatg ctggtgaccg acctgacgct gcgttttgat cctgagttcg
     2641 agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc tttgcccgtg
     2701 cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgctacatc gggccggaag
     2761 tgccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac aacccgaccg
     2821 agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct gttagtgagc
     2881 gggtatcggt ggcctgggca tctgcttcta ccttccgtgg tggcgacaaa cgcggtggtg
     2941 ccaacggtgc gcgtctggca ttaatgccgc agcgcgactg ggatgtgaac gccgcagccg
     3001 ttcgtgctct gcctgttctg gagaaaatcc agaaagagtc tggtaaagcc tcgctggcgg
     3061 atatcatcgt gctggctggt gtggttggtg ttgagaaagc cgcaagcgcc gcaggtttga
     3121 gcattcatgt accgtttgcg ccgggccgcg ttgatgcgcg tcaggatcag actgacattg
     3181 agatgttcga gctgctggag ccaattgctg acggtttccg taactatcgc gctcgtctgg
     3241 atgtttccac caccgagtca ctgctgatcg acaaagccca gcaactgacc ctgaccgcgc
     3301 cggaaatgac cgcgctggtg ggcgggatgc gcgtactggg tgccaacttc gatggcagca
     3361 aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc gtgaacttgc
     3421 tggatatgcg ttacgagtgg aaagcgaccg acgaatcgaa agagctgttt gaaggccgtg
     3481 accgcgaaac cggcgaagtg aaatacactg ccagccgtgc ggatctggta tttggttcta
     3541 actctgtcct gcgtgcggtg gctgaagttt acgccagcag cgatgctcac gagaagtttg
     3601 ttaaagactt cgtggcggca tgggtgaaag tgatgaacct cgaccgtttc gacctgctgt
     3661 aatctgacct tcttcagcga ctgcctttca ggcagtcgct gaagtttctt taccggcgta
     3721 tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga aagagcaacc
     3781 cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc tggattttca
     3841 tgaagcaagt caccagctac atcggtgcct tcgactttac cgccttacgc tgcattttcg
     3901 gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc ccgacaccgt
     3961 ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggc ctggcgcagt
     4021 gggcgctggt cagcggcgga gcggggaagg tggcgatcct aagctatacc atgccgttct
     4081 gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg caatatttcg
     4141 cgatactgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa ctcgatttct
     4201 cttcgatgaa aagtgccatg ctggcaatcc tctccggcgt cagttggggg gcgagcgcga
     4261 ttgttgctaa acgtctgtat gcccgtcatc cgcgcgtgga tttattgtcg ttaacatcct
     4321 ggcagatgct ctatgcggcg ctggtgatga gtgtggtcgc tttactggtg ccgcaacgtg
     4381 aaattgactg gcagcccacc gtgttctggg cgctggccta cagtgcgatt ctggcgacgg
     4441 cactggcgtg gagcttatgg ttgtttgtat tgaaaaactt gcctgccagt attgccagct
     4501 taagcacact ggccgttccc gtttgcggcg tactcttttc ctggtggctg ctcggcgaga
     4561 atccgggggc cgttgaaggt agcggtattg tgctgattgt gatggcactg gcgctggtga
     4621 gccgtaagaa aaaagaagcc gttagtgtaa aaaggatctg aattttttct tcatgtgggg
     4681 cgatcgctta tttaacaaaa taacgataat gccccaccat gcgccagcta aacagcacat
     4741 cttcttcctg cgcacctgcg ccaatgttat gtatcaccag cggcgtaccg tcgcgggcga
     4801 agccatctga aaccacccca atatgtgcca gcccgttatc taatcgccag gagacaatat
     4861 cgcccgcttg atagtcactg gggttcttgc tgatggggcg tgttttatcg tggtgggtaa
     4921 accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg cgctttaact
     4981 gccatttttg cgggtactcg gcaaaattct tcgccatatc ttcgtgcacc agtttctgca
     5041 aatcgacctt ctggctgcgc aatgcacgga tcaccacatc ggaacatacc ccgcgttttt
     5101 gcgggacatc accgccagga taggtaagct gcatatacgc cggatcgtaa aatagcgtgc
     5161 tgccgatttg ctgtctggca ccgtctgcga tggcaaggtt ggtattggcc tggatttgta
     5221 ccacggttgg tggaacggcg ggagatttta aggagtggct ggtaaatgcc gttagcaggc
     5281 tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga aaataagagt cacagattcg
     5341 accttccggg ataacgattg ttctgtagag aaaatgtcca gaagcggata aaaaactcct
     5401 gaacaacgtg ggagtttcag attactggca cagagtgccg gatgcggcgt gaacgcctta
     5461 tctgtcctaa aaaaaaagaa aattcaatat attgcagaga ttgcgtaggc ctgataagcg
     5521 tagcgcatca ggcaattttg cgttcaaact cccggacaag ccgggagttt ggagtaggtt
     5581 attcccactc ttgcaggaaa cgctgaccgt actggtcggc taccagcaga gcggcgtaaa
     5641 cctgatctgg cgtcgcgccg ccaggcatgt tgtggatggt ttcacattct gcacatgccg
     5701 cttcagccac aattcgcatt ttcgccggga catcttcttt aatatccagt tgagcgagag
     5761 ttattggcaa acctaccgca tggctaagtg cagctacggt ttcgatttcc tcaaccggtg
     5821 cgttttccag aaccagctgc gtcagcgtac caaatgccac tttttcaccg tgataatagt
     5881 gatgcgcgtc cgggatagcg gtcaggccgt tatgcactgc gtgcgccgca gccagaccac
     5941 cactttcaaa accaacaccg ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg
     6001 gagtcactac atgctgttcg gcagcaagca tcgctttttc gccttcttcc agcagggtgt
     6061 tgtagcacag ttcagccagt gccagcgcag cctgggtgca cttgccgccc gccatggtgg
     6121 tcgcgccgct acgagagcag gcacgcgctt caaaccaggt tgccagcgca tcgccgatac
     6181 ccgccgctaa cagacgtgca ggtgcgccag cgacgatttt ggtgtcgaca atgaccatat
     6241 tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg tagataacag
     6301 acaatgcgct gcacggtgca tcggtagagg cgatagtcgg tgcgatcgct accggaacac
     6361 ccatgaaatg tgccagtgct ttggctgtat cgagggtttt tccgccaccg ataccgagaa
     6421 ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac
     6481 atccaccgcc aaacggcgca atttctacta ccagtccagc atctttaaag cttttctcga
     6541 cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag cgttctgcca
     6601 gcggcttcag gtattcgccc agacgattaa tcacatcagc gccctggatg tatttacccg
     6661 gtgattgaat aatgcggtcc ataattgctc ctttagagat gagtagtgcc aaatgcggca
     6721 ttccagtcgt gttcgaactt ctctatagct gactctaccg caggggtgtt gagcatttgt
     6781 tgcgctacat ctaagggcag ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc
     6841 tgacgcggcg ttttaaagct ggctgccagc accatgcttt ctggcgcgtg catttctaac
     6901 agcgcttgca gctcctgaac cgtacgaatg ccgtctccgc cctgggcatc tacgcggtta
     6961 acatacggag caacgtattt tgcccctgcc agcgcggcca gtaacccttg tgcagcactg
     7021 tacactgcgg tgccaagcgt agtaataccc tcttttttca gtattttaat tgctgccaga
     7081 ccttcggaag tcaccgggat tttcaccaca atacccggaa tagcgtcgcg caggcgcttc
     7141 gcttcttcca ccatcccctg cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca
     7201 tcaccaatcg ctttttgcag acgcggcagc acttcccata tggactcctt gctggcagcg
     7261 ataatgctcg ggttagttgt caccccggca atggggaata tgcgtgccag acgttcgact
     7321 tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac agttccagtt
     7381 catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc gcgttacgga
     7441 attcttcgtg catgatgcgg cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg
     7501 cagcgtgttt gttcagggtt aacatgatga tgaattgcgc ttcatcatca ccccagcgca
     7561 ccggcgcttg cagacgcgca acgctgatgg tggattgctc aatgtgttct gatttgctgt
     7621 gtggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc gcccacaagt
     7681 cggcttccag tttacgcgga tagcggcagc gccctgccag cagcaggtta tcggtcatgc
     7741 ctttgagcac ttcttctttg ctgcgccagt cgctttccag tgtgatgcac tcggcggtga
     7801 ccagcggtgc gtcttgttgg gtcatgcgga attgcgccag caggtgctcc acttccagcg
     7861 aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta tcaagttgcg
     7921 ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca tccagcccta
     7981 agccgaccag caacggcagc acggaacctt tcgctcccag ctcaccgcac agaccaatcc
     8041 atttaccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat
     8101 tcaggctgtt gtagtgacga gtaaccttag cgttatcgcg atccactgcc agcagatatt
     8161 gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat tgatcgatga
     8221 tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca tcaaacggaa
     8281 tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttctttcacc cataagatct
     8341 cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagcg gaggcgcgca
     8401 ggatcgagcg tagttgtgtg gtaaataacg acgcgtattc ttcataaata cgcacggcgc
     8461 gataaccgag gaacgggttt gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc
     8521 cgccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc agcgcctgac
     8581 aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata tagagcattt
     8641 cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc gcttccacgg
     8701 agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgg gcttgttggg
     8761 tcagccagac acgctgttgc tcacgcaggg cgtcctgtac gcgggcttct tgctgataat
     8821 aacgagctac ggcttctcct ggctcaacca caatcgcccc ggcgttgccg tcgatataaa
     8881 tcgtttgatg ctgccacgga gtgagggcat caatatccac accaaccagc gtcggaatgt
     8941 tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccacttttc agcaacagac
     9001 ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca tcagccatac
     9061 aaatggcgga ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct
     9121 ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg tagctgctgc
     9181 tggaacgggc gaactcttcg caaaagtgat tcgcactggc gacaatcgct tcggcgcagc
     9241 ttaatccctc gctgacgcct gccagtaagt gctgacgtaa agatgtatcg ccagccaggg
     9301 atcggtgagc ttccagtatc gcgctggtag caccgtcgct atccagcaga cgaaactcaa
     9361 tgtttttcag taccagcgtc aggccgtttt ccagtgtgga ttgctcggcg tcaacgtctt
     9421 tggctgcggg aagattactc agcgcattga gatctaaaga agagatcggc gtcagaatgc
     9481 cgcctgcact accgctgcac acggtgcggg cgcggataat ttgcggattc agattggtca
     9541 gtgaaaccgg cagtggttcc agttcgtcag atttaacttc cgccagcggc gcatcgcagt
     9601 gggggaattc atcgcgcagc cattggctta aacgctggtg cgcttcctgt tcgtcggccc
     9661 cggaaatcag taactggcag ttatcgcccg ccagcgtatc ggtgccaatc agcgcaaggg
     9721 cgcttttggc gttgcccttg cggtcagtgt gcaggttatg ccactcaatt tgtgatgaaa
     9781 aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgtacgccg ttaggtagct
     9841 cacaaataaa ttccacaatc agggccattg cctctctccc ggtacggttt tctgtttaca
     9901 ggataagagc gcacggcaac ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg
     9961 taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc atgcttccgg
    10021 tttatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa gtccagtaaa
    10081 tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttcct ccatcaaaaa
    10141 tatggacatg ggctttcgca agcccgggag cagcttatga atgagttggt gcagatcctg
    10201 aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt cgtggtatcg
    10261 ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc
    10321 gtagccgatc caaatctgaa aaaactgttt gatatcggcg ttgcgggctt gacgctgatg
    10381 gtgcctttcc tcgccgctta cattggttat tccattgcag aacgttctgc gctggctcca
    10441 tgcgctatcg gtacctgggt tggtaacagc tttggtgcgg gcttctttgg tgcactgatc
    10501 gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccggt gcataaagtt
    10561 ctgcgctcgg tgatgcctat cttcattatt cctatcgtcg gcacactgat taccgcaggc
    10621 atcatgatgt ggggcttggg cgagcctgta ggggcgttga ccaacagcct gactcagtgg
    10681 cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg tctgatgctg
    10741 gcgttcgata tgggcggtcc ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt
    10801 gctcagggtg tttataccgt ggtggccatc gctgccgtgg gtatttgtat cccgccgctg
    10861 gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc
    10921 ggcaaagcgg cactggtgat gggctgtgtt ggcgttactg gaggggcgat tcctttcgcc
    10981 gctgccgatc cgctgcgtgt cattccttcc atcatggtcg gttcagtttg tggtgcagta
    11041 actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat tgtgctgccg
    11101 gtggttgaag gcaagctggg ttatatcgca gcagtggctg tcggagcagt ggtgacggct
    11161 gtttgtgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa
    11221 gaagacgacc tggatttgga ttttgaaatt aattaattga ggaacgagcc atgacgaaaa
    11281 ttattgcagt aaccgcatgt ccttcaggtg ttgcccatac ctatatggca gcagaggcgc
    11341 tggaaagtgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacctag gggtcaattg
    11401 gtctggaaaa tgaactgact gcggaagatg tggcgagcgc tgatatggtg attctgacca
    11461 aagatatcgg catcaagttt gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca
    11521 tcagcgatgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct cacctggcgc
    11581 aaaccgctta agttcttctc ccgctcgcaa gggcgggttc gctttcccac aggagttcct
    11641 catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc gtgaaatctc
    11701 gctggagcgg gcgctgcttt ataccgccag ccatcggcaa accgaaggcg aaccggtgat
    11761 attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat gttgaaattt cgattcgtga
    11821 tgaagaactg attgccggta accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga
    11881 aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg
    11941 ctttgctatc agcgaagaag ataaacgtat ctaccgtgaa gagttgttcc cgtactggga
    12001 aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac
    12061 caacacgcag attttcagca tcaaccagac ggataaaggc caggggcaca ttattattga
    12121 ttacccacgc ctgttgaatc acggtctggg tgagctggtg gcacagatgc agcaacattg
    12181 tcagcaacag ccggagaatc acttttatca ggccgcgttg ttactgctgg aagcctcgca
    12241 gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaaact gcacagatgc
    12301 acagcgtcgc gaagaactgc tgactattgc ggagatctct cgccataacg ccgaacataa
    12361 gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca ttctgcaata
    12421 cgaatccaac gccagttcgc tgtctttagg gcgcttcgac cagtatatgt tgccgttcta
    12481 tcaggcatct ttaacccagg gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt
    12541 atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agcagcgcgc gttatttcgc
    12601 aggtttcccg accggctata ccgcactgct cggcgggtta accgagaacg gacgtagcgc
    12661 ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa agcgtgcaat taccgcaacc
    12721 gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga aaaccgccga
    12781 aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg tggtaccagc
    12841 gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt
    12901 ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt ttaatctgct
    12961 gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg
    13021 tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg ttgagggcag
    13081 caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctgctct cgtcttttat
    13141 cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc
    13201 cggcgtacag gggatcggta tcgccaacct gagcgattct ctccatgcgt tgaaagggat
    13261 ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa aagccaactt
    13321 tgcaacgcca gaaggcgaaa aagtccgtgc tcgcttaatt aaccgcttcg agaaatacgg
    13381 taacgatatc gacgaggtgg ataacatcag cgccgaactg ttacgccact actgcaaaga
    13441 agtggaaaaa taccagaacc cgcgcgcggt tacttcacgc cgggatcgta taccgtttct
    13501 gctcacgtcc cgttgggatc ggtggttggc gcgacgccag acggtcgttt tgccggggaa
    13561 caactggcag acggcggctt gtcacctatg ctgggccagg acgcacaagg gccaacagcg
    13621 gtactgaagt cagtcagtaa gctcgataat acgctgctgt ctaacggtac gttgctgaac
    13681 gtgaaattca ctccggcgac cctggaaggt gaagcgggat tacgcaaact ggccgacttc
    13741 ttacgggcgt ttacccagct taagttgcag catattcagt ttaacgtggt gaacgccgac
    13801 acgttgcggg aagcgcaaca gcgcccacaa gattatgccg ggctggtggt gcgcgttgcc
    13861 ggatacagcg ccttctttgt cgaactgtcg aaggagatcc aggatgacat catccgccgg
    13921 acagcgcatc agctgtaacg ttgtggaaac gcgccgcgat gatgtggcgc gcattttcaa
    13981 cattcagcgt tattcactga atgacggcga gggcattcgt acggtggtct tttttaaagg
    14041 ctgtccgcat ctttgcccgt ggtgtgctaa tccggagtcg atctccggca aaatccagac
    14101 ggtacgcaga gaggcgaaat gtctgcactg tgcgaaatgt ttgcgtgatg cggatgaatg
    14161 cccctccggg gcgtttgaac ggattggtcg cgatatcagc cttgacgctc tggaacggga
    14221 agtgatgaaa gatgacattt tctttcgcac gtccggcggc ggcgtcacgc tttctggcgg
    14281 cgaagtgtta atgcaggcgg agtttgctac ccgtttttta cagcgactgc ggctgtgggg
    14341 tgtgtcatgc gccattgaaa ctgccggaga cgcgccagca agcaagctgt taccgctggc
    14401 gaaattgtgc gatgaagtgc tgttcgattt aaaaattatg gacgcgactc aggcgcggga
    14461 tgtggtgaag atgaacctgc cacgcgtgct ggagaatctg cgtttgctgg tgagtgaggg
    14521 tgtcaacgtg atcccgcgtt taccgttgat ccctggtttc acgctcagca gggagaatat
    14581 gcagcaggcg ctggatgtac tgatcccgct gaaaatcagg cagatccatc tgttaccgtt
    14641 tcatcagtac ggcgaaccga aataccgcct gctggggaaa acatggtcga tgaaagaggt
    14701 gcctgcgccg tcgtcagccg atgtgacaac gatgcgcgaa atggcagaac gggccggatt
    14761 tcaggttacc gtgggaggtt aaaatggcat acctggtggc agtaaccgcc tgcgtaagcg
    14821 gcgtggcgca tacttatatg gcggcggaac ggctggaaaa gttgtgccag ttagagaagt
    14881 ggggcgtcag cattgaaact cagggcgcgc tgggaacgga gaatcgttta gcggacgagg
    14941 atatccgtcg ggcggatgtt gctctgttga ttacggatat cgagcttgcc ggtgccgagc
    15001 gatttgaaca ttgccgctat gtgcaatgca gcatctacgc attcctgcgt gagccgcagc
    15061 gggtaatgag cgcggtgcgc aaagtgcttt ctgcgccgca gcaaacccat cttattctgg
    15121 agtagtcggt ttttctgtca gttggctgtg gtactgccgg cgatattccg acggcgagcg
    15181 ttcggtgttt ttacgaaaca gacggcagaa gtagttgctg tcgacaaagc cgcaggcgtg
    15241 cgccacttct tttaccttca ggtcatagcc tttcaataac gtcttagcgt gctccagtcg
    15301 cgtgtgattc aggtattcgt taaaaccaat ggcccccgtt ttttgaaaca ggtgcgagag
    15361 atagtttggt gaaatataga atgcctgtgc gacagattcg cgggtaagcg cggaggcata
    15421 gcgttcgtcg atataatcgc gaatagcttc aaatagtgcc tggctgcgtg aggcggtctg
    15481 gatttggctg ccgagcaaat cgcggcagtg gctaagcagg ctggctacga taagccttgc
    15541 cgtttgctgc tcctgcggct gcatttgcat ttcattgagc gtttgtagca gaaaagaacc
    15601 tatacgtggg ccgcgtcggg cgacgtgttg cttcgccaga ttttgatatt gtttgccatc
    15661 ccattgcacg acgctgaagc cgagttgctg tttgccaaac agcacgctaa aggtggtagc
    15721 gggtgttttc cattgtggga aattccagcc accagcggca acgtacagca catcgccggg
    15781 aactaacgtt gctccagcgc cggtatcaac aaactcacct tccagcacaa tttccagccg
    15841 tggaaaatcg acctgatacg caagatcggg caccgggccg ttactgctgg caaagtaaat
    15901 ctggcgcagg gacagcgggc cgttgatcag gcgggagagc agatagctga cgccgtgata
    15961 catgttattc ccttaggtat gtagacggga aaacagtaac tggcattatt aaaaaaggcc
    16021 acctgaaaag tgtggcctga ggggagttcg atgtaaacat tgcggcagtt gtattgccgg
    16081 atgtggcgta aacgccttat ccagcctacc gccgcgatct gtaggccgga taagacgcgt
    16141 caagcgtcgc atccggcaaa tagcgcctgg ctgataatta ctgattacct acctggtcgc
    16201 catacggcag agtgtcgtaa tcgatcagcg cgtttttctt gtacgggtta ccaatccagc
    16261 gggtagtttc tttgaactgc ggattcacca ctgaacgggt tggatcgtaa ccgtcgtaag
    16321 ataagcccgc caaatctgac caggtgtgga tcagttccgc caggctgtat ttacgatcga
    16381 catcctgtga gaaatcacgg ggatgagtcg cttgccattt ttccgacgtc cacagcagga
    16441 acggaatggt gtacatgtgg cgcgtcgggt tgtcttcgtt acgcccctga gttttgtgcg
    16501 gcggcgtgtc gtaaacctct tcaccgtggt cagagaagta aaccaggaat ccgttcgggt
    16561 ctgccgcttt aaagtcttta atcaggctgg caaccacatg atcgttatac aggttagcgt
    16621 tgtcataatc gttatatgat tccagctctt ctgcgcttaa tcctggcgga acatgatcgg
    16681 tattgccatc aaacttgccc tgattttccg ggtagcggta tttgtatttg atatgcgtac
    16741 ccagcagatg aacgatgatc agtttcttcg gcgcagggtc attcagcaca tcctggaacg
    16801 gcttcagcac gttggtgtcg tattcacgcg cactctgcgt acgttgctgg ttcatgtagt
    16861 actgcttgtc ggtctggcgc gaaaataccg tcagcatggt attgcgggcg gtcatcgtct
    16921 gctggttggt gatccagaag gttttataac ccgcctgttt catcatgttc atcagcgacg
    16981 gctgcgtcag atacagatcc gggttctttt cattggcaaa ggtcagcgcc tgttgcagga
    17041 tttcaatggt gtacggacga gacgtaacta cgttattaaa cacggtcaga ttcggatcgg
    17101 ttttatgcag cgcatccagc tccggcgtgg tttcacgcgg ataaccgtac agactcatgc
    17161 gtccgcgctg ggtcgactcg ccaatcacca gcactaaagt gcgcggttcg ttacccgatt
    17221 catctttgaa attagccagt ggcggcaagg cattattttc attcagtaac tttgttagcg
    17281 agtttagttg ctgacgatac tgataatagc cggtcaggaa ttgccacggt gcggcaggct
    17341 ccatacgcga ggccaggtta tccaacgttt tctcaaacgg cttgtttttg ataaacgtat
    17401 tcatggcgat cggatgcaga atcaagccat aaagcagggc aaaagagaca acataacgcc
    17461 acggctttgg aatatagacc gggcgcaggc gtgtccacag cagaactgcc accgccgtat
    17521 aggccagcgc gataagcaca attttcaggc tgaaatactg gcttaaatac tcgctggctt
    17581 cgttggtgtt ggtttcgaac atcacaaaca gaacgctctg cgagaactcc tgcccgtaga
    17641 tgacgtagta gcatagcgcc gccagagagg ccgcccatag caccacgccg attactgcgg
    17701 caataatttt aatccgcttc ggaaagagga ataccgggat caaccacagc gagctgaata
    17761 acagcgagtc gcgaatgccg ttggtgccac tataaccact gatgtaaata atggcctgta
    17821 gcagagtaga gaaaaaccaa aagtagagca gtgcccaacc cagggctttc cagctaaaaa
    17881 ggggtttagc ctggacttct gtggaatgca tagtaagaac ctgtcttgaa aaaaatatcg
    17941 ccgaatgtaa cgacaattcc ttaaggatat ctgaaggtat attcagaatt tgaataaaat
    18001 gcagacagaa atatattgaa aacgagggtg ttagaacaga agtatttcag aaaaccctcg
    18061 cgcaaaagca cgagggtttg cagaagagga agattagccg gtattacgca tacctgccgc
    18121 aatcccggca atagtgacca ttaacgcttg ttcgacgcga ggatccggtt cctggccttc
    18181 tttttctgcc tggcgggagc ggtgcagcaa ctcggcctgc aatacgttca gcgggtcggt
    18241 gtaaatattc cgtagctgaa tagactctgc aatccacggc agatcggcca tcagatggga
    18301 atcgttggca atcgccagca ccactttgat gtcttcttct tgcaggttgc gtaactcttt
    18361 acctaacggc cacagtgctt tgtctaccag acgttgatca tagtattccg ccaaccacag
    18421 gtctgctttg gcgaagacca tctccagcat gccgagacgc gtcgagaaga atggccaatc
    18481 gcggcacatg gcttccagct cgctctgttt gccatcttcg accacttttt gcagcgccgt
    18541 acctgcaccc agccaggcgg ggagcatcag gcggttttgc gtccaggcaa aaatccacgg
    18601 tatggcgcgc agcgactcga cgccgccggt tgggcgacgt ttcgccggac gtgaacccaa
    18661 cggcagtttg cccaattctt gttccggcgt agcggagcgg aagtaaggca caaaatcttt
    18721 gttttcacgt acgtagccac ggtagagatc gcaggagatg actgacagtt catccataat
    18781 gcgacgccag ctctctttcg gctcaggtgg tggcagcagg ttggcttcca gaatcgcccc
    18841 ggtgtaaagc gacaggctgc tgacggtgat ttctggcaga ccatatttaa agcggatcat
    18901 ctcgccctgt tcagtcacgc gcaggccacc tttcaggctt cctggcggtt gtgacagcag
    18961 cgccgcatgg gcaggtgcgc cgccgcgacc aatggaaccg ccgcgaccgt ggaacaacgt
    19021 cagctcaata cccgcttttt cgcaggtttt gattaatgca tcctgtgcct gatattgcgc
    19081 ccaggaagct gccatcacgc ccgcatcttt tgctgagtcg gaatagccaa tcatcaccat
    19141 ctgtttgccc tgaatcaggc cgcgatacca gtcgatattg agcagctggg tcatgacatc
    19201 gttggcgttg ttcaggtcat cgagggtttc aaacagcgga gcaaccggca tcgcaaaccc
    19261 gatacccgct tctttcagca gcaggtggac agccagtacg tcggacggcg ttttcgccat
    19321 cgagatcacg taggcggcaa tggagccttg cggcgcttcg gcaatcacct ggcaggtatc
    19381 gagcacttcg cgcgtttcgg cgcttggttg ccagttgcgc ggcagaagcg gacgtttgga
    19441 gttcagttcg cggatcagga acgcctgttt gtcggcctct gaccagcttt cgtagtcgcc
    19501 gataccgagg tagcgggtca gctcgcccag cgcttcggta tggcgggtgc tctcctgacg
    19561 gatatcaata cggaccagcg gtacgccgaa acatttcacg cggcgcaggg tgtcgagcag
    19621 atcgccgttg gcgataatac ccatgccaca cgcctgaagt gactggtagc aagcgtagag
    19681 cggttcccac agttcttcgt tttgtgtcag cagcccttct ggttttggca gttcttcgcc
    19741 tttcaggcgc gcttccagcc atgcctgtgt cgccatcagg cgagaacgca ggtttttcat
    19801 cagatagcga tacggttctg cggcaccttc ttcgccaacc agcgccagca gttcaggggt
    19861 cgcttcaacc atcgacagtt cagaaaccag aacctgaata tctttcagga acagatcggt
    19921 ggctttccag cgactgagta gcaggacgtg gcgggtgata tcggcagtga cgttcgggtt
    19981 gccgtcgcgg tcaccgccca tccacgaagt aaaacggacc ggaacaaatt cgacgggcag
    20041 tttgtagccg aggttctctt ccagttgttc gttcagttcg cgcaagtaat tcggtacgcc
    20101 ttgccacagg ctgttttcta ctacggcaaa gccccatttg gcttcatcta ccgggcttgg
    20161 acgcagctta cggatttcat cagtatgcca tgactgggcg attaactggc gcaggcgacg
    20221 catcagctgg ttgtgttcgt agtcagcgat atctttgtta tcgagctgtt ttaagcaggc
    20281 gttcacttcc accattttgt ggatcagtgt acgacgggta atttcggttg ggtgagccgt
    20341 gaggaccagt tccagcgaca gcgattccac tgcttttttg atggtgtctt cgctcagttc
    20401 cggctggttt ttcagtttgc gcagggtgcg ggcgatcact tccgggttgc tggcagcttc
    20461 gcctttcggc gaaatgctgt ggtattgctc ggcggtgttg gccaggttca ggaactgact
    20521 aaacgcacgc gcaacgggca gcagctcgtc gttcgacaaa ttttgtaagg tggtgagcaa
    20581 ctcctggcgg ttagcatcat tgccagcgcg tgaagatttc gacaacttac ggatagtttc
    20641 tacgcgttca agaatgtgtt ctcccaacgc atccttgatg gtttctccca gcactttgcc
    20701 gagcatactg acattactac gcaatgcgga atattgttcg ttcatattac cccagacacc
    20761 ccatcttatc gtttgatagc cctgtatcct tcacgtcgca ttggcgcgaa tatgctcggg
    20821 ctttgctttt cgtcgtcttt tataaagcca cgtaaaagcg gtgacgtcaa atgctgcgaa
    20881 atcgcttcag caaacgaata aatagcagga atttacgcca ttaaattcac gacgctttaa
    20941 ataagcgtaa cttatggaaa tgttaaaaaa tcgccataca taacaccaaa ggtgtaggtc
    21001 ggataagatg cgcaagcatc gcatccgaca ttattgcggc accggagttt ggcaacggtg
    21061 ccggatgcgg cgcgagcgcc ttatccggcc tacgttttaa tgccagcaaa aatggtgaat
    21121 tacctggata atcaactcac gggtgggttt aataaaccgc gtctccagat attcatcagg
    21181 ctgatgagct tgattgatgg aacccggacc gagcaccagc gtcgggcata acgtttgaat
    21241 aaacggcgct tcggtacagt aattcaccac ttcggttttt gctccgagca atttctcaac
    21301 cacttcaacc agttgatgat tcggtgggca ttcatagcca gggatcggcg gatgcagctc
    21361 gtcgaccgtc agacgacccg gccagcgttc gctcaccgga gccaatgcat cgttgagcaa
    21421 accattaagt tcattgagtg tcatgctagg cagcggacga atatccatat gcaactcaca
    21481 gcaagcgcaa atacggttag aagcgtcgcc accgtgaata tgcccgaggt tgagcgtagg
    21541 gtatggcacg gtaaatgctt cgtagtgata acgttctttc aggttatcgc gcaattgcaa
    21601 aatatgcccg atggcgtcgt gcattagttc gatagcgtta actccgcgtg ctggatcgct
    21661 ggagtgcccc gactggccct gaatacggat ggcgttagag atatgacctt tatgtgcgcg
    21721 taccggttgt agtgacgtcg gttcgccaat gatggcgcaa tccgggcgca gggcggtagt
    21781 ttcggcaaaa taacgcgctc cggccatact ggtttcttca tcagcagtcg ccagaatgta
    21841 gagcggtttt ttcagtttcg tgacgtcgac atcgcgtagc gtatcaagga taaacgcaaa
    21901 aaagcctttc atgtcggcgg tgcctaagcc gtaaagcttg ccgtcatgct ccgtcagtgt
    21961 aaacggatcg cgcgtccagc gaccgtcatc aaatggcacc gtatcggtat gccccgccag
    22021 caacaagccg ccagccccct gtccgatact tgccagcata ttgaatttgt tgcgagttcc
    22081 tggcacaggc tgcacttcca cattgaagcc taaatcttta aaccagtccg ccagcagagt
    22141 gattaaatct gcattgcttt gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc
    22201 aatcagagca cggtaaatct cgataaatgg cggtaatttg tttttcattg ttgacacacc
    22261 tctggtcatg atagtatcaa tattcatgca gtatttatga ataaaaatac actaacgttg
    22321 agcgtaataa aacccaccag ccgtaaggtg aatgttttac gtttaacctg gcaaccagac
    22381 ataagaaggt gaatagcccc gatgttgaat acgctgattg tgggtgccag cggctacgct
    22441 ggcgcagagc tagtgaccta tgtaaatcgc catccgcata tgaacataac cgctttgact
    22501 gtttcagcgc aaagcaatga tgcgggaaag ttaatctccg atttgcatcc gcagctaaaa
    22561 ggcatcgttg agctgccgtt gcagccgatg tcggatatca gcgagtttag cccaggggtg
    22621 gacgtagtgt ttctcgccac cgcccatgaa gttagccacg atttagcgcc gcagtttctt
    22681 gaagcgggct gcgtggtgtt cgacctttcc ggtgcgtttc gtgttaacga cgccaccttc
    22741 tatgaaaaat attacggctt tacccatcaa tacccggaac tgctggaaca ggcagcctac
    22801 ggtctggcgg agtggtgcgg taataaatta aaagaagcga atttgattgc ggtgccaggc
    22861 tgttatccga cggcggcaca gctggcgctg aaaccgttga ttgatgccga tcttcttgac
    22921 ctcaatcagt ggccggtgat caacgccacc agcggcgtga gcggtgcagg acgtaaggca
    22981 gcgatttcaa atagtttttg tgaagttagc ctgcaaccgt atggcgtctt tactcatcgc
    23041 catcaaccag agatcgccac acacctcggt gctgacgtta tcttcacccc acatctgggc
    23101 aatttcccgc gcggcattct cgaaaccatt acctgccgcc tgaaatcggg tgtgacccag
    23161 gcgcaagtcg cgcaagtgtt acagcaggcg tatgcccata aaccgctggt gcggctgtat
    23221 gacaaaggcg ttccggcgct gaaaaatgtc gttgggctgc cattttgcga tatcgggttt
    23281 gccgttcagg gcgagcatct gattattgtg gcgaccgaag acaacttact gaaaggcgcg
    23341 gcggcacaag cggtacagtg cgccaatatt cgtttcggct atgcggaaac gcagtctctt
    23401 atttaagggt gcaatgatga atccattaat tatcaaactg ggcggcgtac tgctggatag
    23461 tgaagaggcg ctggaacgtc tgtttagcac actggtgaat tatcgtgagt cacatcagcg
    23521 tccgctggtg attgtgcacg gcggcggttg cgtggtggat gagctgatga aagggctgaa
    23581 tctgccggtg aaaaagaaaa acggcctgcg ggtgacgcct gctgatcaga tagacattat
    23641 caccggagca ctggcgggaa cggcaaataa aaccctgttg gcatgggcga agaaacatca
    23701 gattgcggcc gtaggtttgt ttctcggtga cggcgacagc gtcaaagtga cccagcttga
    23761 tgaagagtta ggtcatgttg gactggcgca gccaggttcg cctaagctta tcaactcctt
    23821 gctggagaac ggttatctgc cggtggtcag ctccattggc gtaacagacg aagggcaact
    23881 gatgaacgtc aatgccgacc aggcggcaac ggcgctggcg gcaacgctgg gcgcggatct
    23941 gattttgctc tccgacgtca gcggcattct cgacggcaaa gggcaacgca ttgccgaaat
    24001 gaccgccgcg aaagcagaac aactgattga gcagggcatt attactgacg gcatgatagt
    24061 gaaagtgaac gcggcgctgg atgcggcccg cacgctgggc cgtccggtag atatcgcctc
    24121 ctggcgtcat gcggagcagc ttccggcact gtttaacggt atgccgatgg gtacgcggat
    24181 tttagcttaa gttttgttgg ccggaggcgc agctttccgg cattgaattt caaaataagg
    24241 aaacagagtt atggcacttt ggggcgggcg ttttacccag gcagcagatc aacggttcaa
    24301 acaattcaac gactcactgc gctttgatta ccgtctggcg gagcaggata ttgttggctc
    24361 tgtggcctgg tccaaagccc tggtcacggt aggcgtgtta accgcagaag agcaggcgca
    24421 actggaagag gcgctgagcg tgctgctgga agatgttcgc gccaggccac aacaaatcct
    24481 tgaaagcgac gccgaagata tccatagctg ggtggaaggc aaactgatcg acaaagtggg
    24541 ccagttaggc aaaaagctgc ataccgggcg tagtcgtaac gatcaggtag cgactgacct
    24601 gaaactgtgg tgcaaagata ccgttagcga gttactgacg gctaaccgac agctgcaatc
    24661 ggcgctggtg gaaaccgcac aaaacaatca ggacgcggtc atgccaggtt acactcacct
    24721 gcaacgcgcc cagccggtga cgttcgcgca ctggtgcctg gcctatgttg agatgctggc
    24781 gcgtgatgaa agccgtttgc aggatgcgct taagcgtctg gatgtcagcc cattaggctg
    24841 tggcgcgctg gcgggaacgg cctatgaaat cgaccgtgaa cagttagcag gctggctggg
    24901 ctttgcttcg gcgacccgta acagtctgga cagcgtttct gaccgtgacc atgtgttgga
    24961 actgctgtcg gctgccgcta tcggcatggt gcatctgtcg cgttttgctg aagatctgat
    25021 tttctttaac accggcgaag cggggtttgt ggagctttct gaccgcgtga cttccggttc
    25081 atcattaatg ccgcagaaga aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg
    25141 tcgggtgcag ggtgcgttaa ccggcatgat gatgacgctg aaaggtttgc cgctggctta
    25201 caacaaagat atgcaggaag acaaagaagg tctgttcgac gcgctcgata cctggctgga
    25261 ctgcctgcat atggcggcgc tggtgctgga cggcattcag gtgaaacgtc cacgttgcca
    25321 ggaagcggct cagcagggtt acgccaacgc caccgaactg gcggattacc tggtggcgaa
    25381 aggcgtacct ttccgcgagg cgcaccatat cgtgggtgaa gcggtggtgg aagccattcg
    25441 tcagggcaaa ccgctggaag atctgccgct cgacgagttg cagaaattca gcccggtgat
    25501 tggcgaagat gtctatccga ttttgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa
    25561 aggcggcgtc tcaccgcagc aggtggccca agcgattgct tttgcgcagg cgcggttggg
    25621 gtaagaacat ttatatgtac aaatttgagc ctggcttatc gccgggcttt tttatggcaa
    25681 aaaaaagcgg atcctggaga tccgcaaaag ttcacgttgg ctttagttat tcgagttgag
    25741 aaactctcga aacgggcagt gacttcaagg gttaaaagag gtgccgctcc gtttctgtga
    25801 gcaattatca gtcagaatgc ttgataggga taatcgttca ttgctattct acctatcgcc
    25861 atgaactatc gtggcgatgg aggatggata atgaatattc gtgatcttga gtacctggtg
    25921 gcattggctg aacaccgcca ttttcggcgt gcggcagatt cctgccacgt tagccagccg
    25981 acgcttagcg ggcaaattcg taagctggaa gatgagctgg gcgtgatgtt gctggagcgg
    26041 accagccgta aagtgttgtt cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc
    26101 gtgctgcgtg aggtgaaagt ccttaaagag atggcaagcc agcagggcga gacgatgtcc
    26161 ggaccgctgc atattggttt gattcccacg gttggaccgt acctgctacc gcatattatc
    26221 ccgatgctgc accagacctt tccaaagctg gaaatgtatc tgcatgaagc acagacccac
    26281 cagttactgg cgcaactgga cagtggcaaa ctcgattgcg tgatcctcgc gctggtgaaa
    26341 gagagcgaag cattcattga agtgccgttg tttgatgagc caatgttgct ggctatctat
    26401 gaagatcacc cgtgggcgaa ccgcgaatgc gtaccgatag ccgatctggc aggggaaaaa
    26461 ctgctgatgc tggaagatgg tcactgtttg cgcgatcagg caatgggttt ctgttttgaa
    26521 gccggggcgg atgaagatac acacttccgc gcgaccagcc tggagacgct gcgcaacatg
    26581 gtggcggcag gtagcgggat cactttactg ccagcgctgg ctgtgccgcc ggagcgcaaa
    26641 cgcgatgggg ttgtttatct gccgtgcatt aagccggaac cacgccgcac tattggcctg
    26701 gtttatcgtc ctggctcacc gctgcgcagc cgctatgagc agctggcaga ggccatccga
    26761 gcaagaatgg atggctattt cgataaagtg ttaaaacagg cggtttaaac cgtttaacgc
    26821 agctacccga taggcttccg ccatggtcgg gtagttaaag gtggtgttga cgaagtactc
    26881 aatagtgttg ccgccacctt tctgttccat aatcgcctga ccgatatgaa taatttcggc
    26941 agcgcgctcg ccaaagcagt gaatacccag aatctctttt gtttcccgat ggaacaaaat
    27001 tttcagcgtg cccacgttca tgccgacgat ttgtgcgcgt gccagatgtt taaactgggc
    27061 gcggcccact tcatatggca ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga
    27121 gctgatttcc gggatggtgt aaatgccggt agggatatct tcaatcagat gcgcggtggc
    27181 ttcgcctttt accagcgcct gcgcggcaat gcgcccctgg tcataggccg ctgatgccag
    27241 gctcggataa ccaatcacgt cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat
    27301 gctgttgacc ttcagctgtc cgcgactgtc agtttccagc ccaatgttct gtaacgccag
    27361 cgaatcggta ttaccggtac gaccgttggc atagagcagg cagtcagctt tcagtttttt
    27421 acccgacttc agatgcatga tcacaccgtc gtcacagcct tcgatcttct cgtactcttc
    27481 gttgtgacga atcactacgc cactgttcca gaagtgatag gagagagaat ctgacatctc
    27541 ttgatcgaga aatgccagca ggcgatcgcg ggtgttgatc agatccactt ttacatccat
    27601 accgcggaag atcgacgcat attcacagcc gatcactcca gcaccataga taagtacatg
    27661 gcgcggttcg tggtgcatgc taagaattga gtcgctgtcg taaatgcgtg gatgggtgaa
    27721 atcaacatct gttggatgat atggacgaga gccgcaggca ataacaaatt tttcagcggt
    27781 tagtgtttcg acgctgccgt ccgggcaatc cagcgccaac gtatgctcgt caacaaagcg
    27841 agcgttcccc tgcaatattt cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt
    27901 ttgttgatta atcacgttat cggcatggtt aaggatatcg gcaaaagaag agcggagcag
    27961 tcgcgaatgg tcgctgtata gtgggttttg attgaattct ataatgcggc tgacggcgtg
    28021 acggagagct ttcgacggga tggtgcccca gtgggtgcaa ccgccgccaa cattttgata
    28081 acgctcgata actgcgacgc gcacaccttg cttaaccagg cccattgcag cgccttcgcc
    28141 gccggggccg gaacctatta ctatggcatc gtaatcgtag gaatgtggca tggtagggct
    28201 tacctgttct tatacataaa agcaacagaa tggtaacatt ctatcgcggg taagccaatt
    28261 gatccccgtc atttatctgg ctatatcctg agcggccttt gctttgtctg tttcttactt
    28321 ttgccctgac gttttattgg atttttatcg acgatactct ccgtttaagc ggcaggtttc
    28381 cgctgtacgt aaaagaaccg gccagtaaat atgttttatt gcgttaccgt tcattcacaa
    28441 tactggagca atccagtatg ttcattctct ggtatagtgc cagcagtact tttggcaagg
    28501 attcagacat cgtgatgggc ataagagcgc aacaaaaaga aaaaacccgc cgttcgctgg
    28561 tggaagccgc atttagccaa ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg
    28621 aagtggcgcg tgaagcgggc attgctccca cctcttttta tcggcatttc cgcgacgtag
    28681 acgaactggg tctgaccatg gttgatgaga gcggtttaat gctacgccaa ctcatgcgcc
    28741 aggcgcgtca gcgtatcgcc aaaggcggga gtgtgatccg cacctcggtc tccacattta
    28801 tggagttcat cggtaataat cctaacgcct tccggttatt attgcgggaa cgctccggca
    28861 cctccgctgc gtttcgtgcc gccgttgcgc gtgaaattca gcacttcatt gcggaacttg
    28921 cggactatct ggaactcgaa aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag
    28981 caatggtgac aattgtcttc agtgcgggtg ccgaggcgtt ggacgtcggc gtcgaacaac
    29041 gtcggcaatt agaagagcga ctggtactgc aactgcgaat gatttcgaaa ggggcttatt
    29101 actggtatcg ccgtgaacaa gagaaaaccg caattattcc gggaaatgtg aaggacgagt
    29161 aatgaaacaa gcaaatcaag atagaggtac gctgctgctg gcgttagttg ctggcttatc
    29221 gattaatggt actttcgcag cgctgtttag ctccattgtg ccattttctg tattcccgat
    29281 tatttccctg gtgctgacgg tttactgcct gcatcaacgt tatcttaatc gcactatgcc
    29341 ggtaggcttg ccgggtctgg cagctgcctg ttttattctc ggcgtactgc tgtacagcac
    29401 ggtagttcgt gcggaatatc cggatatcgg ctctaacttc ttcccggcag tactctccgt
    29461 cattatggtg ttctggattg gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta
    29521 atcggtatta tgccggatgc tggcgcatcc ggcatgggtt ttacttcgcg gtcagtaata
    29581 cgccgcactc catatggtgc gtgtagggga actgatcaaa cagagccaga cgttcgacct
    29641 tgtgcgtctg gcttaatgtt tccagattct tgcataacgt ttccgggtta caggagatgt
    29701 acaaaataca cggatacgcc tgcaccattt tctcggtttc actgtccaga ccgctgcgcg
    29761 gtgggtcgac aaaaatggtt tcgcactgat aactctttaa gtcgatccct tgcaggcggt
    29821 taaactcgcg cacaccattc atcgcctgag taaattcttc tgccgccata cgaataattt
    29881 gtacgttatc aatatggtta gctgcgatgt tgtattgcgc agcagcaacc gacggcttag
    29941 cgatttcggt ggctaatacc cgatcaaaat tgcgcgccag cgctaatgaa aagttaccgt
    30001 tgccgcagta cagctccagt aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt
    30061 ccagcatctg aatattcatc gccgcgttcg gctgggtaaa gctgttttct acctgacggt
    30121 agatcatctc tttccctgcg accggcagac gctcgtcgat gtaatcttga tccagctcga
    30181 ttttggtttt cgttgcccga ccaatcagat gcacattcag attctgcgcg cgcagtgcat
    30241 cgcgcagggc ctccgcttcc tgacgccact catcatccag cttcttatgg tatagcaggg
    30301 aaaccaccgc ctgattactc agcgtggtga ggtaatcaat ctggaacaac ttgtggcgca
    30361 gaacgggatt attacgcaca cccgcaatca tcgccgtcat caactggttg ataagttcac
    30421 tggcggcggg gaagctatcc acgcggatac ggcttttggt ttgttgatcg aaaatgatgt
    30481 gatacaggtc atcgccatcg tgccagatgc ggaactccgc acgcatccgg taatgactga
    30541 ccggcgagcg aaacacttcc ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac
    30601 gtaccacttt ttcggctaac tgcgcttcat actgttctgt tggaaggtgt tcgggggtca
    30661 tgatgtatcc tgaaaaatta aagtacgcgg cgattgtagg gattgctcat cagatgtcca
    30721 gatcttgatg aattcctatt tgtgagctac gtctggacag taacttgtta caacctgtag
    30781 catccacttg tcggtcctgt gagttaatag ggaatccagt gcgaatctgg agctgacgcg
    30841 cagcggtaag gaaaggtgcg atgattgcgt tatgcggaca ctgccatccg gtgggaagtc
    30901 atcatctctt agtatcttag atacccctcc aagcccgaag acctgccggc caacgtcgca
    30961 tctggttctc atcatcgcgt aatattgatg aaacctgcgg catccttctt ctattgtgga
    31021 tgctttacaa tgattaaaaa agcttcgctg ctgacggcgt gttctgtcac agccttttcc
    31081 gcttgggcac aggataccag cccggatact ctcgtcgtta ctgctaaccg ttttgaacag
    31141 ccgcgcagca ctgtgcttgc accaaccacc gttgtgaccc gtcaggatat cgaccgctgg
    31201 cagtcgacct cggtcaatga tgtgctgcgc cgtcttccgg gcgtcgatat cacccaaaac
    31261 ggcggttcag gtcagctctc atctattttt attcgcggta caaatgccag tcatgtgttg
    31321 gtgttaattg atggcgtacg cctgaatctg gcgggggtga gtggttctgc cgaccttagc
    31381 cagttcccta ttgcgcttgt ccagcgtgtt gaatatatcc gtgggccgcg ctccgctgtt
    31441 tatggttccg atgcaatagg cggggtggtg aatatcatca cgacgcgcga tgaacccgga
    31501 acggaaattt cagcagggtg gggaagcaat agttatcaga actatgatgt ctctacgcag
    31561 caacaactgg gggataagac acggggaacg ctgttgggcg attatgccca tactcatggt
    31621 tatgatgttg ttgcctatgg taatacagga acgcaagcgc agacagataa cgatggtttt
    31681 ttaagtaaaa cgctttatgg cgcgctggag cataacttta ctgatgcctg gagcggcttt
    31741 gtgcgcggct atggctatga taaccgtacc aattatgacg cgtattattc tcccggttca
    31801 ccgttgctcg atacccgtaa actctatagc caaagttggg acgccgggct gcgctataac
    31861 ggcgaactga ttaaatcaca actcattacc agctatagcc atagcaaaga ttacaactac
    31921 gatccccatt atggtcgtta tgattcgtcg gcgacgctcg atgagatgaa gcaatacacc
    31981 gtccagtggg caaacaatgt catcgttggt cacggtagta ttggtgcggg tgtcgactgg
    32041 cagaaacaga ctacgacgcc gggtacaggt tatgttgagg atggatatga tcaacgtaat
    32101 accggcatct atctgaccgg gctgcaacaa gtcggcgatt ttacctttga aggcgcagca
    32161 cgcagtgacg ataactcaca gtttggtcgt catggaacct ggcaaaccag cgccggttgg
    32221 gaattcatcg aaggttatcg cttcattgct tcctacggga catcttataa ggcaccaaat
    32281 ctggggcaac tgtatggctt ctacggaaat ccgaatctgg acccggagaa aagcaaacag
    32341 tgggaaggcg cgtttgaagg cttaaccgct ggggtgaact ggcgtatttc cggatatcgt
    32401 aacgatgtca gtgacttgat cgattatgat gatcacaccc tgaaatatta caacgaaggg
    32461 aaagcgcgga ttaagggcgt cgaggcgacc gccaattttg ataccggacc actgacgcat
    32521 actgtgagtt atgattatgt cgatgcgcgc aatgcgatta ccgacacgcc gttgttacgc
    32581 cgtgctaaac agcaggtgaa ataccagctc gactggcagt tgtatgactt cgactggggt
    32641 attacttatc agtatttagg cactcgctat gataaggatt actcatctta tccttatcaa
    32701 accgttaaaa tgggcggtgt gagcttgtgg gatcttgcgg ttgcgtatcc ggtcacctct
    32761 cacctgacag ttcgtggtaa aatagccaac ctgttcgaca aagattatga gacagtctat
    32821 ggctaccaaa ctgcaggacg ggaatacacc ttgtctggca gctacacctt ctgaaccacg
    32881 tcccaccgtg ctggtgtttg actccggcgt cggtgggttg tcggtctatg acgagatccg
    32941 gcatctctta ccggatctcc attacattta tgctttcgat aacgtcgctt tcccgtatgg
    33001 cgaaaaaagc gaagcgttta ttgttgagcg agtggtggca attgtcaccg cggtgcaaga
    33061 acgttatccc cttgcgctgg ctgtggtcgc ttgcaacact gccagtaccg tttcacttcc
    33121 tgcattacgc gaaaagttcg acttcccggt tgttggtgtc gtgccggcga ttaaacctgc
    33181 tgcacgtctg acggcaaatg gcattgtcgg attactggca acccgcggaa cagttaaacg
    33241 ttcttatact catgagctga tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg
    33301 ctcggcagag atggttgagt tggctgaagc gaagctacat ggcgaagatg tttctctgga
    33361 tgcactaaaa cgtatcctac gcccgtggtt aagaatgaaa gagccgccag ataccgttgt
    33421 attgggttgc acccatttcc ctctactaca agaagaactg ttacaagtgc tgccagaggg
    33481 aacccggctg gtggattctg gcgcagcgat tgctcgccga acggcctggt tgttagaaca
    33541 tgaagccccg gatgcaaaat ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc
    33601 agaagctgaa caattattgc ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact
    33661 ggcagtttta ggctgatttg gttgaatgtt gcgcggtcag aaaattattt taaatttcct
    33721 cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac
    33781 acgccgccgg gtcagcgggg ttctcctgag aatc
//
LOCUS       DAZOZD010000020        32520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOZD010000020 DAZOZD010000000
VERSION     DAZOZD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.020"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            153..1526
                     /gene="aroP"
                     /locus_tag="ACHUPB_000761"
     CDS             153..1526
                     /gene="aroP"
                     /locus_tag="ACHUPB_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0757657.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1569..2423)
                     /gene="ampE"
                     /locus_tag="ACHUPB_000762"
     CDS             complement(1569..2423)
                     /gene="ampE"
                     /locus_tag="ACHUPB_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0757658.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2420..2971)
                     /gene="ampD"
                     /locus_tag="ACHUPB_000763"
     CDS             complement(2420..2971)
                     /gene="ampD"
                     /locus_tag="ACHUPB_000763"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0757659.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3059..3952
                     /gene="nadC"
                     /locus_tag="ACHUPB_000764"
     CDS             3059..3952
                     /gene="nadC"
                     /locus_tag="ACHUPB_000764"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0757660.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4155..4595
                     /gene="ppdD"
                     /locus_tag="ACHUPB_000765"
     CDS             4155..4595
                     /gene="ppdD"
                     /locus_tag="ACHUPB_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0757661.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4605..5991
                     /gene="gspE"
                     /locus_tag="ACHUPB_000766"
                     /pseudo
     CDS             4605..5991
                     /gene="gspE"
                     /locus_tag="ACHUPB_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            5981..7183
                     /gene="hofC"
                     /locus_tag="ACHUPB_000767"
                     /pseudo
     CDS             5981..7183
                     /gene="hofC"
                     /locus_tag="ACHUPB_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7218..8261)
                     /gene="guaC"
                     /locus_tag="ACHUPB_000768"
     CDS             complement(7218..8261)
                     /gene="guaC"
                     /locus_tag="ACHUPB_000768"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0757662.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8417..8461)
                     /locus_tag="ACHUPB_000769"
     CDS             complement(8417..8461)
                     /locus_tag="ACHUPB_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757663.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8486..9106
                     /gene="coaE"
                     /locus_tag="ACHUPB_000770"
     CDS             8486..9106
                     /gene="coaE"
                     /locus_tag="ACHUPB_000770"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0757664.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9106..9849
                     /gene="zapD"
                     /locus_tag="ACHUPB_000771"
     CDS             9106..9849
                     /gene="zapD"
                     /locus_tag="ACHUPB_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0757665.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9859..10056
                     /gene="yacG"
                     /locus_tag="ACHUPB_000772"
     CDS             9859..10056
                     /gene="yacG"
                     /locus_tag="ACHUPB_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0757666.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10156..10545)
                     /gene="mutT"
                     /locus_tag="ACHUPB_000773"
     CDS             complement(10156..10545)
                     /gene="mutT"
                     /locus_tag="ACHUPB_000773"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0757667.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10605..13310)
                     /gene="secA"
                     /locus_tag="ACHUPB_000774"
     CDS             complement(10605..13310)
                     /gene="secA"
                     /locus_tag="ACHUPB_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0757668.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13372..13884)
                     /gene="secM"
                     /locus_tag="ACHUPB_000775"
     CDS             complement(13372..13884)
                     /gene="secM"
                     /locus_tag="ACHUPB_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0757669.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14115..15032)
                     /gene="lpxC"
                     /locus_tag="ACHUPB_000776"
     CDS             complement(14115..15032)
                     /gene="lpxC"
                     /locus_tag="ACHUPB_000776"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0757670.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15133..16284)
                     /gene="ftsZ"
                     /locus_tag="ACHUPB_000777"
     CDS             complement(15133..16284)
                     /gene="ftsZ"
                     /locus_tag="ACHUPB_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0757671.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16345..17607)
                     /gene="ftsA"
                     /locus_tag="ACHUPB_000778"
     CDS             complement(16345..17607)
                     /gene="ftsA"
                     /locus_tag="ACHUPB_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0757672.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17604..18434)
                     /gene="ftsQ"
                     /locus_tag="ACHUPB_000779"
     CDS             complement(17604..18434)
                     /gene="ftsQ"
                     /locus_tag="ACHUPB_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0757673.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18436..19356)
                     /gene="ddlB"
                     /locus_tag="ACHUPB_000780"
     CDS             complement(18436..19356)
                     /gene="ddlB"
                     /locus_tag="ACHUPB_000780"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0757674.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19349..20824)
                     /gene="murC"
                     /locus_tag="ACHUPB_000781"
     CDS             complement(19349..20824)
                     /gene="murC"
                     /locus_tag="ACHUPB_000781"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0757675.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20878..21945)
                     /gene="murG"
                     /locus_tag="ACHUPB_000782"
     CDS             complement(20878..21945)
                     /gene="murG"
                     /locus_tag="ACHUPB_000782"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0757676.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21942..23186)
                     /gene="ftsW"
                     /locus_tag="ACHUPB_000783"
     CDS             complement(21942..23186)
                     /gene="ftsW"
                     /locus_tag="ACHUPB_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0757677.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23186..24502)
                     /gene="murD"
                     /locus_tag="ACHUPB_000784"
     CDS             complement(23186..24502)
                     /gene="murD"
                     /locus_tag="ACHUPB_000784"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0757678.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24505..25587)
                     /gene="mraY"
                     /locus_tag="ACHUPB_000785"
     CDS             complement(24505..25587)
                     /gene="mraY"
                     /locus_tag="ACHUPB_000785"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0757679.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25581..26939)
                     /gene="murF"
                     /locus_tag="ACHUPB_000786"
     CDS             complement(25581..26939)
                     /gene="murF"
                     /locus_tag="ACHUPB_000786"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0757680.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26936..28429)
                     /gene="murE"
                     /locus_tag="ACHUPB_000787"
     CDS             complement(26936..28429)
                     /gene="murE"
                     /locus_tag="ACHUPB_000787"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0757681.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28416..30182)
                     /gene="ftsI"
                     /locus_tag="ACHUPB_000788"
     CDS             complement(28416..30182)
                     /gene="ftsI"
                     /locus_tag="ACHUPB_000788"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0757682.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30198..30563)
                     /gene="ftsL"
                     /locus_tag="ACHUPB_000789"
     CDS             complement(30198..30563)
                     /gene="ftsL"
                     /locus_tag="ACHUPB_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0757683.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30560..31501)
                     /gene="rsmH"
                     /locus_tag="ACHUPB_000790"
     CDS             complement(30560..31501)
                     /gene="rsmH"
                     /locus_tag="ACHUPB_000790"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0757684.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31503..31961)
                     /gene="mraZ"
                     /locus_tag="ACHUPB_000791"
     CDS             complement(31503..31961)
                     /gene="mraZ"
                     /locus_tag="ACHUPB_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0757685.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7720 a         9141 c         8024 g         7635 t
ORIGIN      
        1 gctgccgcat accattattc ttgatctgac ggaagtcttt ttgtaacaat tcaaacttct
       61 ttgatgtaaa aaaattaata caacaaacgg aattgcaaac ttacacacgc atcactgcgt
      121 agatcaaaaa aacaatcacc gcacgaggtt tcatgatgga aggtcaacag cacggcgagc
      181 agctaaagcg cggccttaaa aaccgccata ttcagcttat cgcgctgggt ggcgcgatag
      241 ggacagggct attcctgggt agcgcctccg taatacagtc cgcagggcca gggattatcc
      301 tgggttacgc cattgctggt tttatcgcct ttctgatcat gcgtcagctg ggtgaaatgg
      361 tggtcgaaga acctgtcgca ggctccttta gccactttgc ttataaatac tggggcagtt
      421 ttgccggttt cgcctctggc tggaactact gggtactgta cgttttagtt gccatggcag
      481 agctgactgc cgtgggtaaa tacattcagt tctggtatcc ggaaatccca acctgggttt
      541 ctgccgccgt attctttgtg gtgattaacg ccatcaacct gaccaacgtt aaagtgtttg
      601 gcgagatgga gttctggttt gccattatca aagttattgc gatagtagcg atgatcatct
      661 tcggcggctg gctgctgttc agtggcaacg gcggcccgca ggcgagcgtt agcaacctgt
      721 gggatcaagg tggtttcctg ccgcacggct tcaccggact ggtgatgatg atggcgatta
      781 tcatgttctc gttcggtggt ctggaactgg tggggatcac cgcagcagaa gctgataacc
      841 cggagcaaag tatcccgaaa gcgactaacc aggttatcta ccgcatcctg attttctata
      901 ttggttcgtt agccgttctg ctctcactga tgccgtggac ccgcgttacc gccgatacca
      961 gtccgtttgt gctgatcttc cacgagttag gcgatacctt tgtggcgaat gcgctgaaca
     1021 tcgtggtact gactgcggcg ctctccgtgt acaacagctg cgtatattgc aacagccgta
     1081 tgctgtttgg tctggcacaa cagggtaatg cgccaaaagc gctggcgtct gtcgataaac
     1141 gcggcgtacc ggttaacacc attctggtgt ctgcgctggt tacagcattg tgcgtattga
     1201 ttaactatct tgctccggaa tccgcatttg gcctgttaat ggcactggtg gtatccgcac
     1261 tggtgatcaa ctgggcgatg atcagtctgg cgcatatgaa gttccgtcgc gccaagcagg
     1321 aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc gctgggtaac tggatctgcc
     1381 tgctgtttat ggcggcggta ctggtgatta tgctgatgac cccaggaatg gcgatttcgg
     1441 tatacctgat cccggtatgg ctgatcgtgt taggtatcgg ctatctgttt aaagagaaaa
     1501 ccgccaaagc cgtaaaagcg cattaatctc tctacgccct cacccatgca gggtgagggc
     1561 aataatcttt acaccaacgc tccgtaaatc gtcagtagcg caatcaccac cacgaccacg
     1621 aacgaggttt tcttcgccat tgaaaccgct gccttcggcg tctccacctt atcgacatgc
     1681 ggttcacgcg ccagagagaa ctgcgccaga cgcgttaaca cctgatactg cgaagtatgg
     1741 aagtcaccca gcgaagcaaa ccaggccggt aacgctttct ccccatgacc gatcaaggca
     1801 tataccacac ccgcaagacg aaccggcacc caatccagta catgaagcac ggcatcaatg
     1861 ccggactgta aacgatgatg cggcgtctgg tatcgcgcca gccagtattg ccatgcacgc
     1921 aaaaacgcat accccatcag cgtaacgggt ccccaggttc cccccacaat caaccagaac
     1981 agcggtgcaa gataaaaacg aaagttaatc cacagcaatg cattttgcag ctcacgcaaa
     2041 tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca tggtgagttc gccagccatc
     2101 gtggcacggg catggctatc attacgtgaa gcagctgtca gataagcatg ataatgaaga
     2161 cgaactttac ctgcgccaat acacagcaaa ccaatcagca gccacaccag cagcgtggga
     2221 acgttgaaca atactccctg caatgcgcgt aacagtaaaa aagtcacgcc catcgcaata
     2281 atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca cccgccgaaa gaacgcttca
     2341 agacgatgat caagctgcca gtgctcgccc aacttaaaca ggcgctcgaa aattaacacc
     2401 agtaaggttg taaatagcgt catgttgtct ccttgctgac cagcgcacga aaccgtgccc
     2461 aatcaaatgc aggaccggga tcggttttcc gatccggcgc aatatcacaa tggcccgtca
     2521 tgttattagc gatatcggga tagcgatcta tcagtgcccg cgtaaccgcc gcaagctgtt
     2581 gatactgcgc atcggtatac gccagagtat cggtgccttc aagctcaatc ccaatagaaa
     2641 aatcattgca gcgttcacgc ccctgatact gagagactcc cgcatgccag gcacgtttat
     2701 cgaaaggaac atactggact atttcaccat cacggcgaat caaacagtga gcggagacgc
     2761 gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg atcaatagtt ccagtgaata
     2821 atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag gctaatattg tgcaccacca
     2881 gcagggtggg tgtttcgtca tccgggcggc aatcgtaatg tggtgaggga acgcggcgcg
     2941 cgccaaccag ccacccctgt tctaacaaca tgcaggatct ccttatatgt ggtgctaata
     3001 cccggttcag agtagcatgt ttctacctta tgattcgtta gctatctgga gttttaacat
     3061 gccgcctcgc cgctataacc ctgacacccg acgtgacgag ctgctggaac gcattaatct
     3121 cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat ttaggcggaa cagtcgatgc
     3181 caacaatgac attacggcaa aacttttacc ggaaaactct cgctctcatg ccacggtgag
     3241 cacccgcgag aatggcgtat tttgcggcaa acgctgggtt gaagaggtgt ttattcaact
     3301 ggcaggcgac gatgtcacca taatctggca tgtggatgac ggcgatgtca tcaacgccaa
     3361 tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg ttaacgggcg aacgcactgc
     3421 gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag gtacgccact atgtcgaatt
     3481 actggaaggc accaacacgc agttgttgga tacgcgcaaa accttacctg gcctgcgttc
     3541 agctctgaaa tacgcggtac tttgcggcgg cggagcgaat caccgtctgg ggctttctga
     3601 tgccttcctg atcaaagaaa atcatattat tgcctccggc tcagtgcgcc aggccgtcga
     3661 aaaagcgtcc tggctgcacc cggatgcgcc agtagaagtc gaagtagaga atctggaaga
     3721 acttgatgaa gccctgaaag caggagccga tatcatcatg ctggataact tcgaaacaga
     3781 acagatgcgc gaagccgtca aacgcaccaa cggcaaggcg ctactggaag tgtctggcaa
     3841 cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc gtggacttta tctccgtcgg
     3901 tgcgctaact aaacacgtac aagcactcga cctttcaatg cgttttcgct aattgctgac
     3961 agatttatcc gctccatcgc gggcggataa tctccccttc cccaactttc ttcgtaacgc
     4021 ctcgcaaatt tcttgtcttt tgctgcaacg aaataaaaaa cctcggaagc tgctttcccg
     4081 tttcgttttt tgcccctcgc ttgtgctgat ggcgtttgcc aaagtagcgc caaccaaatc
     4141 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
     4201 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
     4261 aagctgcact caccgacatg ctacaaacct ttgtgcctta ccgtacagcc gtagagttgt
     4321 gcgcgctgga acatggtgga ttagatacct gcgacggtgg cagcaatggc attccctcgc
     4381 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa aggcgtggtg tcgctgaccg
     4441 ggcaagaaag tctcaatgga ctaagcgtcg tcatgacacc gggttgggat aacgcaaacg
     4501 gcgtcaccgg ctgggcgcgc aactgcaata ttcaaagtga cagcgcattg cagcaagcct
     4561 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
     4621 tcaccgccct gtgtctgcgt tatcagggag tcttgctgga tgccagcgaa gaggtggttc
     4681 atgttgcggt agtcgatgca ccttcgcatg agttgctgga cgcattgcat ttcgctacca
     4741 ccaaacgtat tgagatcacc tgctggacgc gccaacaaat ggaaggccac gccagtcgca
     4801 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctga
     4861 ctcgaacgtt acaatctgcg ctggaacaac gtgcgtctga tattcatatc gaaccagcgg
     4921 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggatgttt
     4981 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg
     5041 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
     5101 catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa ggtggtatta aggttgttac
     5161 agcaggtgaa ccaggcactg gatgttaaca cgctgggaat gcagccgtta caactggcgg
     5221 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
     5281 gcggcaaaac ggtcacgctt tatagtgccc tgcaaaagct gaataccgct gacattaata
     5341 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg actaaaccag acgcaaatcc
     5401 atccgcgtgc cgggctcacc tttcagggcg ttttgcgtgc gttattgcgc caggatcctg
     5461 acgtcatcat gatcggagag atccgctatg gctttaacgg cagaaattgc cattaaagcc
     5521 gcgcaaaccg gtcacctggt gttgtctacc ctacacacta attccacctg cgaaacgctg
     5581 gtacgtttac agcaaatggg agtcgcccgc tggatgctct catcagcgct tacgctggta
     5641 atagcccagc gtctggtacg taaactttgc ccacattgtc gccagcagca aggggagccc
     5701 atccatattc cagtcaatgt atggccgtcg ccgctgcccc actggcaggc acccggttgt
     5761 gtacattgct accacggttt ttatggtcgt acggccttat ttgaagttct gcccataacg
     5821 ccggtcattc gtcagcttat ttccgctaat accgacgttg aatcgctgga aacgcacgca
     5881 cgacaggcgg gtatgcgtac gctttttgaa aacggctgcc tggccgtgga gcaaggctta
     5941 accacctttg aagagttaat ccgcgtactg gggatgccgc atggcgagta agcaactctg
     6001 gcgctggcat ggcataaccg gcgacggcaa tgcgcaagat gggatgctat gagcagagag
     6061 ccgtgctttg ctgctcatgg cactacagca acagatggtt accccactta gcctgaagcg
     6121 aatcgccatc aattctgcgc agtggcgagg agataaaagc gcggaagtca ttcatcaact
     6181 ggcgacgcta ctcaaagccg ggttaacgct ttctgaaggg ctggcactgc tggcggaaca
     6241 gcatcccagt aagcaatggc aagcgttgct gcaatcgctg gcgcacgatc tcgaacaggg
     6301 cattgctttt tccaatgcct tattaccctg gtcagaggta tttccgccac tctatcaggc
     6361 gatgatccgc acgggtgaac tgaccggtaa gctggatgaa tgctgctttg aactggcgcg
     6421 tcagcaaaaa gcccagcgtc agttgaccga caaagtgaaa tcagcgttac gttatcccat
     6481 catcatttta gcgatggcaa tcatggtggt tgtggcaatg ctgcattttg ttctgccgga
     6541 gtttgccgct atctataaga ccttcaacac cccactaccg gcactaacgc aggggatcat
     6601 gacgctggca gactttagtg gcgaatggag ctggctgctg gtgttgttcg gctttctgct
     6661 ggcgatagcc aataagttgc tgatgcgccg accgacctgg cttatagtgc ggcagaaatt
     6721 gctgttacgc atcccgatta tgggttcact gatgcgggga caaaaactca cgcagatctt
     6781 tacgattctg gcgctgacac aaagtgcagg cattactttt ttgcagggcg tagagagcgt
     6841 cagagaaaca atgcgctgcc cgtactgggt gcaacttctg acacaaatcc agcacgatat
     6901 cagtaacggt catcccatct ggctggcgct aaaaaatacc ggggagttta gcccgctctg
     6961 tttgcaatta gtgagaacag gagaggcatc cggctcgctg gacctcatgt tagacaacct
     7021 cgcccatcat catcgggata acacaatggc gctggcggat aacctcgcag ccttactgga
     7081 accgacgttg ctgatcataa cgggaggaat tatcggtacg ctggtggtgg cgatgtatct
     7141 gccaattttc catttaggcg atgcgatgag tgggatggga taacgctggc gtgttgctcc
     7201 acaccagcgt tggaagatta caggttgttg aagatgcggt tttcttgttc ctgcacacgg
     7261 ataaatgtgg tgcgcttggt cagttctttc aggcgtgaag ccccaacgta tgtacaagct
     7321 gaacgcaggc cgcccaaaat atcacgcgcg gtattttcaa ccgggcctcg cagcggcagc
     7381 ttaacggttt taccttctgc tgcgcgatat tccgcaacgc cgccaacgtg acgtttcatc
     7441 gcagactcgg agctcatgcc gtagaacagc ataaatttct cgccgttctc ctcaacgatg
     7501 cgaccgccgc tctcttcgtg gcccgccagc atgccgccaa gcatgacgaa atcggcaccg
     7561 ccgccaaagg cttttgcgac atcgcccggc gtggtgcagc caccgtcgct gacgatcatt
     7621 ccgcccagac cgtgcgccgc atcggcacat tcgattaccg cagaaagctg cggataaccg
     7681 acgcctgttt tgacgcgagt tgtacaaaca gaacctgggc caatgccaac tttaacgata
     7741 tcagcaccgg agaggataag ctcctcacac atttcaccag tcactacgtt gccagcacaa
     7801 atggttttgg tcggccacgc ttcacgcgct ttcgcaacga actgcacgaa gtgttcggaa
     7861 taaccattcg ccacatcaat acaaacgaag tttaatgccg ggttcaggtc gagaatctgt
     7921 ttagtttttt cgaaatccgc atcagacgta ccggtagaaa ccatcacatg tttcagcaca
     7981 tcagcggaag aattgttgat aaacgcttgc cactcttcga cagaatagtg tttatgcaca
     8041 gcagtcaaaa tatcaaaaga agccagcgca gaggccatag aaaatgtacc tacggtgtcc
     8101 atatttgcgg cgataatcgg cacgccggac cagctctgac ctgaatgttt gaaggtgaat
     8161 tgacgttcca gttcaacatc ggaacggctt ttaagagtgg agcgtttagg gcggatgaga
     8221 acgtctttaa aacctaactt cagatcttct tcaatacgca tgtgcggatt cctggggtta
     8281 atggctataa gggtaagagc gcaactccag tgacgctatc atacgcacta atcaatgcgg
     8341 cgcaagactg cgaaattgtc tttttttacg ctacaatccc ataaatttac ctggtaaata
     8401 gtgagcgaac gtttcatcaa tttcgctttc gtgcactcat ttttaacgtt ttgataatca
     8461 tgatgaattc ccgggaatat aaattatgag gtatatagtt gccttaacgg gaggcattgg
     8521 cagtggcaag agtaccgttg ccaatgcgtt tgctgatctc ggaattaacg tcattgatgc
     8581 cgatattatt gcgcgtcagg tggttgaacc aggtgcacct gcgctacatg ccattgctga
     8641 tcactttggc gctaacatga ttgctgctga tggaacattg cagcgccggg ccttgcgcga
     8701 gcggatcttc gccaacccgg aagagaaaaa ctggcttaac gccctgctgc atccgctgat
     8761 tcagcaagag acgcaacacc agatccagca agctacttcc ccctatgtac tgtgggttgt
     8821 gccattgctg gtagaaaact cactgtataa aaaagcgaat cgagtgctgg tggtggatgt
     8881 cagcccagaa acgcaactta agcgcaccat gcagcgcgat gatgtaactc gcgagcatgt
     8941 cgaacaaatc cttgctgctc aggcaacgcg cgaagcccgc cttgccgtgg cagatgacgt
     9001 cattgataat aacggcgcac cggatgctat cgcatcggat gttgcccgcc tgcacgcaca
     9061 ctatttgcag cttgcgtcgc agtttgtctc acaggaaaaa ccgtaatgca gacccaggtc
     9121 ctttttgaac atccactaaa tgaaaaaatg cgtacatggc tgcgcattga gtttttgatt
     9181 cagcaactca ccgttaattt acccatcgtt gaccacgctg gcgcgctgca tttcttccgt
     9241 aatgtcagtg aattactgga tgttttcgag cgcggcgaag tccgcactga gctgttgaaa
     9301 gaacttgacc ggcagcaacg taaactccag acctggattg gcgtgcctgg cgtggaccag
     9361 agccgtattg aagcattaat tcagcagtta aaagcggcgg ggagcgtatt aatttccgcg
     9421 ccgcgtatcg ggcaatttct gcgtgaagat cgtttgattg ctctggtgcg tcagcgactg
     9481 agcatcccag gcggctgttg cagctttgat ttacctacat tgcacatttg gctgcatcta
     9541 ccccaggcgc agcgcgacag ccaggtagaa acctggattg ccagcctgaa cccgctcacc
     9601 caggcactta ccatggtgct ggatttaatt cgccagtcgg cccccttccg taaacaaacc
     9661 agtctgaatg gtttttatca ggataacggt ggcgatgccg acttgctgcg cctgaatctg
     9721 tcgctcgatt cacagcttta tccgcaaatt tccggacata agagccgttt tgccattcgt
     9781 tttatgccgc tggacactga aaacggacag gtaccggaac gtctggattt cgaactggcc
     9841 tgttgctaag gagtaaaaat gtcagaaact attacggtga attgcccaac ctgcgggaaa
     9901 acggtggtgt ggggtgaaat cagcccgttt cggccatttt gctccaaacg ttgtcagctg
     9961 atcgacctcg gagaatgggc tgctgaagaa aaacgaatcc ccagcagtgg cgatctttcc
    10021 gaaagcgatg actggagcga agagccaaag cagtgacatt tgtctgatgc cgcacgtagg
    10081 cctgataaga cgcggacagc gtcgcatcag gcatcttgtg caaatgtcgg atgcggcgtg
    10141 aacgccttat ccgacctaca gacgcttaag cttcgcaatt accggttcat tggctggcgg
    10201 aaaatcatcg gcattaagac cgactagcga catccactca ccgggttgcc cttctttacc
    10261 ccacggctcc ccttcccagc tttcgaccag ccagaaccac agagttatat gcctgtccgg
    10321 gaattcatat tccagttttt caaatagcga aaaatgttgt ggggtaatcc cgacttcttc
    10381 ctgaagttca cgcactaccg cctgttccgg cgtttcgccc atttcaattt taccgccggg
    10441 aaactccagt ttattcgcca tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt
    10501 gttctcgttg cgaataatac ctaccgcaat ttgcagcttt ttcattgtct taaacctata
    10561 aaaaaggcgc agaatcctgc gccttttact tcaactgtta gcttttattg caggcgacca
    10621 tggcactgct tgtatttttt accagaaccg cacgggcaag gatcgttacg tcctactttg
    10681 cgctcaccgg tttgcgccgc cagtgcagct gcggctgcag agtcgtcatc ctgatggcta
    10741 agctgctgca tttgcgctaa acgctcggct tccatacgac gctgttgttc cagctcctca
    10801 acctcttcag gcatacgtac ctggactttg ctcagcgtgc tgataacttc atattttaac
    10861 gactccagca tcgctgcaaa catggagaac gattcacgtt tgtattcctg cttcggatct
    10921 ttctgtgcat agccacgcag gtggataccc tgacgcagat agtccatcgc cgctaggtgc
    10981 tctttccaca gagagtcgag agtttgcagc atgacgcctt tttcgaagtg acgcatcatc
    11041 tcagcaccaa ccacttcttc tttacgctga tacacttcga tggactgcgc cagaattcgc
    11101 tcacgcagcg tctcttcatg cagttctggt tctttatcca gccactcggc aattggcaaa
    11161 tcgaggtcga aatcgttctt cagacgttcc tgcagccccg gaatatccca catttcttcc
    11221 agtgactgtg gcggaatgta ggcatcaatg gtcgctttga acacatcttc acgaatgctg
    11281 ttgatggttt cgctcacatc gctgacatcc agcagttcgt tacgctggga gtaaatggcg
    11341 cgacgctgat cgttagccac gtcatcatat tccagcagtt gcttacgaat gtcgaagtta
    11401 cggctttcaa ctttacgctg ggcgttggca atcgctttag tcacccacgg gtgttcaatg
    11461 gcttcgcctg gcttcatacc cagtttacgc atcatgccgg atactcggtc ggaagcaaaa
    11521 atacgcatca gcgcatcttc catcgacagg tagaaacggg aagaaccagc atccccctga
    11581 cgaccagaac gaccgcgcaa ctggttatcg atacgacggg attcgtgacg ctcggtaccg
    11641 atgatatgca ggccacctgc ttccagtacc gcatcgtgac gtacctgcca gtcggcttta
    11701 attttttcaa tttgctctgc ggtcggattt tccagcgcgg caacttctgc ctgccagcta
    11761 ccaccgagca caatatctgt accacgaccc gccatattgg tcgcgatagt caccgcagcc
    11821 ggataacctg cctgagcaac aatcgccgct tcgttggcgt ggaatttggc gttcaggacg
    11881 ttgtgcttaa taccggcttt ggtcagttcg tttgacacca gctccgattt ttcgatggag
    11941 atagtaccca ccagcaccgg ctggcctttc gcagtacgtt ctttgatatc ttcaatgatc
    12001 gcctgaattt tttccgcttc agtcatgtag accaggtccg gcagatcttt acgaatcatt
    12061 ggacggttgg tcggaacaac gacggtatcc agcttgtaga tggagctaaa ttcgaaagct
    12121 tcggtatcag cagtaccggt catccccgcc agtttttcat acagacggaa gtagttctgg
    12181 aaggtgatcg aagccagcgt ttggttttcg ttctggatct gcacaccttc tttcgcttcc
    12241 acagcctggt gcagaccatc ggaccagcga cggccctgca tggtacgacc ggtgtgttcg
    12301 tcaacgatga taacttcacc atctttaacg atgtagtcga cgtcgcgggt aaacagcgca
    12361 tgagcgcgca gcgccgccgt tacgtggtgc atcagcatga tgttggccgg agagtacaga
    12421 gactcccctt catccatgat gccctctttc accagcagtt cttcaatcag caccagacca
    12481 cgttcggtca ggttcacctg gcgagatttt tcgtccaccg agaagtggcc ttcgccctgg
    12541 aaggtttcgg agtcttcttt ttcctgacgg atcaggtgcg gaataatttt attcacgcgt
    12601 ttatacattt ccgagctgtc ttctgccggg ccggaaatga tcagcggtgt acgcgcttca
    12661 tcgatcagga tggagtccac ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt
    12721 tcttcagggc tgaacgccat attgtcgcgc aggtagtcaa agccgtattc gttgttcgta
    12781 ccgtaagtga tgtcagctgc gtaagcttcg cgctttgccg gtgctggcat gcccggcagg
    12841 ttgataccga cagtcaggcc aaggaattca aacagcggac ggttgttttc ggcgtcacgt
    12901 tgcgccaggt agtcgttgac ggtaactacg tgcacgcctt taccggttag tgcgttcagg
    12961 taagcaggca gcgttgcggt cagggtttta ccttcaccgg tacgcatttc ggcgatgcag
    13021 cgttcgttaa gaaccatacc gccgagtaac tgaacgtcga agtgacgcat accaaagacg
    13081 cgcttacttg cctcacgtac cacggcgaaa gcttccggga tcagattttc cagcacttcg
    13141 cctttttcca gacgtgcacg aaactctgcg gttttccctt tcagttcttc gtcggagagt
    13201 ttttccatct ccggttccat ggcattgatg atgttgacca ctttgcgcat ccggcgcagg
    13261 gtgcgatcgt tacgactacc gaaaacttta gttaacaatt tgattagcat aataaaatct
    13321 caaacgcccc gcgttgcgga gttaataaaa tgaagtaaag gtttattgtt gttaggtgag
    13381 gcgttgaggg ccagcacgga tgccttgcgc ctggcttatc cagacgggcg tgctgaattt
    13441 tgcttgtggg gtaaaatgcg cataatcaat gcgataaccc ttttcagacg gcgtgccttc
    13501 ctgggtcagc agcgcgctga gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa
    13561 agactcttca gcaacgggca gtgtttgcgg tgccattgcg aaagaaagat gacggattac
    13621 cgtgcgaatg gcatgttgat gccagtaatc aacggaatag ttcgaattcg ggcggcgtgt
    13681 gttcgcttcc agcaaggcca attgaccaaa gttaactttg gctgaaggct cgtggttgcg
    13741 ggttgtcgct tttgcgggcg cgtttggttc ggcggcgttg ctgagcgcag gcaaacctaa
    13801 actcgccgca accatcccta ataagagatg cggccagaag tagcgtttac caaactgtcg
    13861 ccagcgcgtc agtattccac tcacgttatt gccatcccga tcgtattcaa acgtatttag
    13921 cgcacaaatc ttatcattaa agcctgcagt ccacagcacg aatgaagata agttgtgcgg
    13981 aaaagtgctt aagaaaacag cgttcgcacc gttttttata gctcttataa aattcaaaaa
    14041 aaaatgactg gttcacctgg ccggagagag tgccagattt gccagtcgaa ttttatacga
    14101 cagtataaat gtcgttatgc cagtacagct gaaggcgctt tgaaggccaa cggcagttct
    14161 gcgtcgtcct ggaaggtcac atattcccag gcttcctgtt tcgccaggac agcctgcagc
    14221 agtttgttat tcagtgcatg accggattta taagcggtaa atgcaccaat aatattgtga
    14281 ccacacatga acaagtcacc gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca
    14341 aaacgcaggc cgtcttcgtt cagtacgcga taatcgtcaa caacgatggc acaatcgaag
    14401 ctgccgccca ggcacaaacc acgggactgc agatattcga tatcacgcat gaaaccgaac
    14461 gtacgcgcac ggctgatctg gcgcataaac gcatcagcgg agaagttcat cgcatagcgc
    14521 tggttgctgg aatcaatagc cggatggtta aaatcgatgg tgaaatccag cgaaaaacca
    14581 ttgtacggct taaattcagc ccacttatcg ccatcttcga cacgaacagt ctctttgatg
    14641 cgaacaaatt ttttggcgca gttcaactcg tcgataccgg cgtcaagcag caggtataca
    14701 aacggagcgg cgctgccgtc catgatcggg atttccggcg cgttaacttc gataacaatg
    14761 ttatcgatgc ccaggcccgc gagagcagca ttgaggtgct ctacggttga aatccgtaca
    14821 tcatgctcgt tgaccagaca cgtacagagc atggtatcac gcacagattt ggcatcggcc
    14881 gggaaatcta ccggtggatt caagtcggtg cgacgataga tgaccccggt gttggccggc
    14941 gcagggcgta acgtcagagt gactttcttg ccggtatgta aaccgacacc cgtcgcctga
    15001 acgatacgtt taagtgtcct ttgtttgatc atcgtattat ctcgccaaat tacctatcca
    15061 accgaagtgt actatacatt cggcgggcca gtttagcaca aagagcctcg aaacccaaat
    15121 tccagtcaat tcttaatcag cttgcttacg caggaatgct gggatatcca gataatccgg
    15181 ctctttcgca gtttgcggcg cattgtcatt cacgacttta gcaaccggct tctgctcctg
    15241 ggtcagcgga gccatcccat gctgctggta gcgatccatc actggctgct gaacctgctt
    15301 attggtcacc agagtgattt caggacgttt gtccatgccg atacctgtcg caacaacggt
    15361 tacgcgcagc tcgtcattca tatccgggtc aagagaagta ccgataacca cagtcgcgtt
    15421 gtcggaagca aatgcacgga tggtgttacc taccgtttcg aactcatcca gacgcaggtc
    15481 gaagcccgcc gtgatgttaa ccagcacgcc gcgcgcgcca gacaggtcga tatcttccag
    15541 cagcggagaa gagatagcca tttcagcagc ttcttccgca cggtcttcac cgctcgccac
    15601 gccagaaccc atcattgcgt agcccatctc agacattacg gtgcgtacgt ctgcaaagtc
    15661 cacgttcatc aaacccggac gagtaatcag ttcagcgata ccttgcacag cgcctttcag
    15721 tacatcgttc gctgcgccaa acgcatccag cagggagata ccgcggccca gaactttcag
    15781 cagtttgtcg ttcgggatag tgatcagaga gtccacatgc ttggacagtt cagtgatccc
    15841 ctgctccgcg aatgccatac gcttcttgcc ttcaaagttg aaaggcttag tgacgacagc
    15901 aacggtcagg atacccaaat cttttgccac ttcagcgacg actggtgctg cacctgtacc
    15961 ggtaccacca cccatacccg cagcaataaa gaccatgtct gcaccttcca gcgccgcacg
    16021 caatgcatcg cgatcctcat cagccgcatt gcgaccaact tctggattag cgccagcgcc
    16081 cagtcctttg gtgataccgc taccgatttg aatcgtctgt ccaaccgctg ttttacgcag
    16141 cgcttgtgca tcggtattta ccgcgaagaa ttcaacacct tcaatacgct cgcgcaccat
    16201 gtgttcaaca gcattaccgc cgccgccgcc gacgccgatg actttaatca ccgcgtcatt
    16261 ggtaagttcc attggttcaa acatagtttc tctccgattt gtgcctgtcg cctgaggccg
    16321 taatcatcgt cggcctcata aaaattaaaa ctcttttcgc agccaactat tgagtcgctt
    16381 gatccacgag ccaactgatg ctgtaacacg tttttctact tcagcttcac cgttaagatg
    16441 tgactctttc ccatagtgaa gcaatcccac cgccgtcgaa taatacggct cctgagcata
    16501 atccgttaaa ccggtaatgt tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg
    16561 ctgagcacag gctgcaagac cttcgatctg cgctgcgcca ccggttaata caatgcctgc
    16621 cgccaggtga tgtttaaccc cttgttggcg aagcttttcc tgcaactgca atatctcttc
    16681 gttgaccagg ttgagcagct cggtatagcg cggctcgatc acctctgcca gggtctgacg
    16741 ttgcagactc cgtggcggac gaccgcctac gctcggcact tccacgctct catcttttcc
    16801 aacgatggaa cccagcgcac aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg
    16861 cgtgccaaag gcgtaagcga tatcactggt cacgacattg ccagcataag gaattacctt
    16921 agtgtggcgc aatgccccac cggtataaac ggcgatatcc attgtaccac caccgatatc
    16981 gacgacgcag acacccagtt cacgttcatc ttccgtcaat accgaataac ttgatgccag
    17041 tccggcaaat atcagttggt caactttcag cccacaacgt tcaaccgctt tgacgatgtt
    17101 tttcgccata tcgttgtgac atgtgatcag gtgcactttt gcctgcatcc gcacgcccga
    17161 aagtcctacc ggattcttga tcccttcctg atagtcaatc gcatactctt gcgggatcac
    17221 atgcagcaca cgatgttcat cgcgcacacg caccgatttc gcggtatgga cgacgttttc
    17281 cacatcttct tgcgtcactt cttcttcaga aataggcacc ataccaattt cattctggca
    17341 gctgatgtgc ttaccagaaa gcgccagata taccgaagag atctgacaat ctgccatcaa
    17401 ttctgcctgg tcaatggcgc gttgtacgca cttgaccacg gattcgaggt cgttcacccc
    17461 gcctttatcc ataccacgtg acgggcagct gcccacgcca atgatattga ccataccgtc
    17521 gggcagaact tcccctacta aagcggcaac cttcgcggta ccaatctcca gtcctactac
    17581 cagttttctg tccgtcgcct tgatcattgt tgttctgcct gtgcctgatt ttgttgctga
    17641 gtagattcct ctggcggcaa gggcgcccag cctactgccg ctccagagtc ataacgcaaa
    17701 tcaacgtagc taatccgttt gccatcggtt tgcgcctgct gctgtaaaac cggataaagt
    17761 tctacaaagc gagccaaacg tttcatcgta tcgccccggc caagattgag cttaatatcg
    17821 ttattcagcg tcaactgcca ggaacgccgc gcggtcatcg ccgcttcctt cagagtaaat
    17881 ctgtcctttg ccagcatctg ccccatttcg cgatagccct gcaacacttc attggcgctg
    17941 ccttccgggc catacagcat tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg
    18001 aaggtgtttc cttccgcgtc taccatatgt tgatcattcc accgcgcaat cggcacatat
    18061 tcaaccagat gaatcttcaa ttcatcaggc cactgctttc tgacgctcac ctgcttaatc
    18121 cacggcaggc gttgttctat ttgcgtctgg atgatgttga catcctgggt cataaaggta
    18181 cccggctcac ccaatgccag gatcgactgc cggatatcgt cattacgcgt gtaatggcgt
    18241 tcaccggtca acaccagctt tgagagcggc aggcgttgcg catcttccat ccagcccaac
    18301 acgacccagc cgctcaccaa cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc
    18361 agacgcgttc cattattgcg gcgagaagaa acctcttctt cgctatttcg cgtgttcaga
    18421 gcagcctgcg acatattagt ccgccagttc cagaattcgt actaccaact gcgagaagct
    18481 catacctgcc tgacgtgccg ccatcggcac caggctgtgg ctggtcatac ccggtgaggt
    18541 attggcttcc agcagataaa actgtccatc gctgtccagc ataacgtcaa tacgtcccca
    18601 tcctttgcaa cctaacgtcg tccatgcttt cagcactaat gcctgcaaat tggcctcttg
    18661 tgacgcttcc agacctgcgg ggcagaaata ctgtgtctca tcagagagat acttcgcctc
    18721 ataatcatag aaggttccgg acggttgaat acgtattgac ggtaaaattt cttcaccgag
    18781 tatcgcaacc gtgaactccg gcccacttag ccatttttca atcaatactt cttcatcgtg
    18841 ctgaaatgcc aatcttaatg catcttgtag agcattttct gctactactt ttgacattcc
    18901 cacactggaa ccttcgcggc tcggcttaac gataaccggc aaacccagag cagaaatttc
    18961 tgctaactgc ttatcgctca ggcctttttc aaactctgcg cgggttaacg ctacccacgg
    19021 cgcgaccggt aaaccggcac cttgccacag cagtttgctg cgtagtttat ccattgaaag
    19081 cgcagatgcc atcactccgc tgccggtata aggcaagccc atcagctcaa gcatgccctg
    19141 cagcgtacca tcttcaccgc cgcgaccgtg tagcgcgata aacactttct gaaaacccat
    19201 cgacttcagt tgcgtcacgt cgacttcttt cgggtcgaca ggatacgcat caataccgcc
    19261 ttcacgcagc ccggctaaca ccgctgcgcc agaattcaga gaaacttccc gctcagcgga
    19321 agtcccgccc aacaggaccg caattttatc agtcatgttg ttcttcctcc ggagtttgcg
    19381 gcttcagttt gatttcagct aaagaacggg caatttttcc gatattacca gccccctgaa
    19441 cgagaatcag gtcgttaccg attaataccg gtgccagcat ctcggctacc tgcgcagggt
    19501 ccggcaccag aatgggatca attttcccac gtccacgaat tgtgcgacac agcgaacggc
    19561 tgtccgctcc cggaattggc gcttcgccag ccggatacac ttccagcatc aacagggtat
    19621 caacctgcgt cagcacattg gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt
    19681 gcggctgaaa cagcattact aggtttttat ccggccagcc tgcgcgcgcc gctttaatgg
    19741 tggcgtcaac ttccgtcggg tggtggccgt agtcatcgac cagcattgcc gtaccgcttt
    19801 taccattcac tggctccagc gggaattcac cgaggaaatc aaaacggcga ccagtcccct
    19861 ggaagctctc cagcgcacgc aaaatagcct cgtcgtcaat tccctcttcc gtagcaaccg
    19921 caaccgcagc tgcggcgttc agcgcgttat gacggcctgg cgcattcagg gtgacgcgca
    19981 tcggctcttt gtcctggcgc agcagcgtaa agtgcccctg cgggccaatc tgctgataat
    20041 cttctacacg cacgtcggca tcttcgctga agccgtaagt cgtggtctga cgtcccacac
    20101 gcggtaacaa ttcgcggatc accggatcat caacacacat caccgcacga ccgtaaaacg
    20161 gcaggttgtg cagaaaatta ataaaagtct gttttaaatt ctcaaagtcg ccctggtagg
    20221 tatccatgtg gtcggcttcg atattggtga caatcgccac catcggttgc agatgcagga
    20281 acgatgcatc actctcatct gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat
    20341 gaacccccgc cgcttttacc agcccgccgt taacgaaggt tgggtcgagc cccgcttctg
    20401 cgtagatgct ggaaaccatc gcggtggttg tcgttttgcc gtgcgttcct gcaatggcga
    20461 tgccatgacg aaaacgcatt aactcagcca gcatttcggc acgacggatc accggaatac
    20521 gcgcttcatg agctgcgaca atttccgggt tatcggcaga aatggcgctg gaaacaacga
    20581 ccacgctggc atcacgtacg ttttccgggc gatggttgaa ataaatcgtc gctcccagat
    20641 tcattaactg ctgcgtgacc ggatttggcg ctaaatcgga accactgatc tgataacctt
    20701 cattggccag aacttcggca ataccgccca taccggcacc accgatgccg acaaaatgta
    20761 tgtgccgaac gcgacgcatt tcgggcacga tggaacgcag ttttgccaat tgttgtgtat
    20821 tcattcttta cgccattaac ttcttaaatt catacgatgc aaaaggcatc gctacaatta
    20881 cgcccgggca gcccggctca cttcatttgc cactcgctcg gtggcatccg gaatggatgc
    20941 agcgcgggcg cgttctgcca tggttaataa ggtttctcgc gaccacccgg ccagggtgtt
    21001 ggcgacagca tccacgctaa gctgtggctg ctcgataatt ttggctgcgc ccgctttttc
    21061 cagcggtagc gcattccagt attgctggcg gtctttatgt tgaaacggca caaacaacgc
    21121 cggtagtcct gccgcggcga tttcactcac cgttaaagca ccggagcggc aaacgacgac
    21181 atccgcccac gcatacgccg ccgccatatc atcaataaat tccgtcactt tatgctgcgg
    21241 ttgccccgct tcggcatacg cctgttcaac ggattgttgc gaacctttac cgctctgatg
    21301 ccagatagtg actgaatcac ccagtttcgc agcaacctgc ggcattgtct ggttaagaat
    21361 gcgtgcgccc tgggaaccac ccactaccag cacacgaacc ggaccttcac gtccagccaa
    21421 acgttgctgc ggcaacggca gcgccagcac atcggtacgc accgggttac ccaccacttc
    21481 cgcattaggg aaagcgcctg gaaacgcctg catcactttg gtggcaatct tcgccagcca
    21541 tttattggtt aagcccgcaa taccgttttg ttcatgaagt acaaccggaa tgcctaacga
    21601 ccacgcggcc agaccacctg gacctgatac atagccgccc ataccgagta ccacgtcagg
    21661 tttgtacgct ttcataatcg cccgcgcctg acgccaggcg ttgaagatac gcagcggcgc
    21721 agctatcagt gcttttatac cttttccacg cagaccagag atacgaatga aatcaatttc
    21781 gatgccatgt tttggcacta agtccgcttc catacggtcg gcagtcccca gccagcgaac
    21841 ttgccaaccc tgagccatta gatggtgcgc aaccgccagt cccgggaata catgtccacc
    21901 ggttccgcct gccatcacca ttaatcgctt tccttgacca ctcatcgtga acctcgtaca
    21961 aacgcctgcg ctttctccag acgcgtttca taatcaatac gcaacagcat catgatggct
    22021 gtcgacataa tcagtaagct cgaaccaccg taactgatca gcggcaatgt cagacctttg
    22081 gtcggtaaca tccccgccgc cgcgcctacg ttaaccagcg cctggaagct aaaccagatg
    22141 ccaatagaac aggcgagaaa accggaaaaa cggtggtcaa tttctaacgc tttgcggcca
    22201 atcgacatcg cgcgaaaagc gacgaagaat accattaaaa gtgccagcac cacaccgaca
    22261 taccccagtt cttcgccgat aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac
    22321 tccagttttt gtaccgagtt acctaaacct tgtccccaaa gttcgccgcg accaaacgcc
    22381 atcagcgatt gcgttaactg atagccgctg ccaaagggat cttcccacgg gttccagaat
    22441 gcggtaacac ggcggatacg gtacggttcg gcgagtatca gcaacacaac cgctgaaatg
    22501 cccataccga taatggcaat gaactgccac aatttcgctc ccgccaggaa caacatcgcc
    22561 agcgtagtca caaacaacac caccaccgta ccaaggtctg gctgtgccag cagtaacact
    22621 gccaacacca gaatcacgcc catcggtttc aggaagccgc gcaggttatt acgtacttcg
    22681 tcgcctttac gcaccagata gttggcgata taacaaaaca gcgacagttt tgtcagctcc
    22741 gcaggctgga tacgcagcaa accgagatcg atccaacgcg atgccccttt aaccgagcta
    22801 cccactacca gcacgatcat cagcaggatg atagatccga gcagcatcgt ggcactgtag
    22861 cgttgccaga actccatcgg cagacgcagc gtaatgatcg ccagaatgaa tgccaggatc
    22921 agatagacgc catcacgctt cgcgaagaag aacggatcgt tggttaagcg ttgccctatg
    22981 ggcattgacg ccgaggtcac catgataaag ccaatcgccg caaggccgaa ggtcagccac
    23041 agtaaggtgc gatcgtacat gatcaggctg tcggtatctt tttcccgcga gcccatcacc
    23101 cagcctttta gcgccgtgga gatccagacc aggatactga atcctggcag gcgcggcatt
    23161 ttcaggcgag ggagagataa acgcatcaac ctaactcctt cgccagacgg gcaaactcat
    23221 tgcctcgttg ttcaaagttc ttgaactgat caaggctggc acaggccggg gagagcagaa
    23281 ccatatcgcc cagctgaaca cgcggagcca gcaagcgcat cgcttgttcc atggtttcgg
    23341 tttgttctgc cacttccggg cgtagcgccg ccagctgcgc accgtcacga ccaaaacagt
    23401 acaaacgcac gttgtcgcca ttcagatagc gcgccagtgg actgaagtcc gccgatttac
    23461 catcgccgcc cagcaacaaa tgcagcgtgc cgtctacctg caagccattc aacgccgctt
    23521 ccgtactgcc gacgttagtg gctttcgaat cgttaatcca gcgtacgcca ttgtgctcca
    23581 gcaccacttc aaagcgatgc ggcagaccgg tgaaggtggt taacgctttc aggctgctgg
    23641 cgcgcggcaa accagctgcg tctgccagtg ccagtgctgc cagcgcattg gtgtagttat
    23701 gctgcccgga aagtttcatc tctttcacat tcagcacttt ctcgccttta acccgcagcc
    23761 aggtttcgcc ctgctgatga ttcaggtgat agtcacccat gttgacgcca aagctgacgc
    23821 agcgttcatc agcaccgcga atcggcattg ttaaggcatc atcagcattg acgacacaaa
    23881 ctttcgcatt ttcgtaaata cgcaattttg ccgcacggta ttgctgcaaa ccaaacggat
    23941 agcggtccat atgatcttca gtcacgttca gaatggtcgc cgccaccgcc tgtaagctgg
    24001 aagtggtttc cagctggaag ctcgacagtt ccagcacgta cagttcgcac tcagcatcaa
    24061 gcagcatcaa cgctggcagg ccaatattgc cacccacacc aacgttaacc cccgccgctt
    24121 tcgccatttc acccactagc gtggtgaccg tgcttttgcc gttagaaccg gtaatcgcca
    24181 caatcggtgc ttgtgcttcg cgacagaaca gctcgatatc gccaacgatt tcgattccgg
    24241 catcagcggc agcgcttaag gatggatgcg cctgtgcaat accgggactg gcgacaatca
    24301 gatctgccgc catcagccat tcatcattca gactgcccgt gtggcgttct acggcttcgg
    24361 gtaatttatc caggccaggc ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc
    24421 gagcgaggaa aaagtccacg caggaaagcc cggtgaggcc caggccgata atgacgacat
    24481 ttttaccctg ataatcagcc atgattaacg taccttcagc gttgccagac caatcagaac
    24541 cagcatcagc gaaataatcc agaaacgcac aatgacgcgc ggttccggcc agcctttcag
    24601 ttcatagtgg tgatgaatcg gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga
    24661 gccgacctgc aggatgacag aaagcgtttc taccacgaac acgcccccca taatcaccag
    24721 caggaattcc tgacgtagca gtacggcgat aatgcctaac gcaccaccta acgccagcga
    24781 acctacatcg cccataaaga cctgcgccgg ataggtgtta aaccacagga agcccagtcc
    24841 tgccccgact atcgccgtgc agacaatgac cagttcccct gcgtgtcgca gatacggtat
    24901 atgcaagtag ctggcaaagt tcatattgcc ggtcgcccac gccaccagcg caaaaccacc
    24961 ggcgacaaat acggtcggca taattgccag gccgtcgaga ccatcggtca ggtttaccgc
    25021 gttgccagta cccacaatga cgaagtaagc cagcagaatg tagaacagcc ccagctgcgg
    25081 catcacatct ttaaagaatg ggaccaccag ctgcgttgcg ggcgtgtctt tgccggcaag
    25141 gtacagggcg aaggcgacac ccagcgcaat gaccgacatc cagaaatact tccaacgagc
    25201 gatcaaccct ttggtgtctt tacgcaccac tttgcgataa tcatcaacaa agccaataac
    25261 accgtaacct accagcacca ccaacacgca ccagacgtac ggattggacg ggtaagccca
    25321 cagcagtacg gagatcacaa tcgccgtcag gatcataatc ccgcccatgg tcggcgtacc
    25381 gcgcttgctg aagtgtgatt caggaccgtc gttacgcacc acctgaccaa aggaaagttt
    25441 ttgcaaatga gctatcatac gcgggcccat ccacaatgag atgaacagcg cggtcagcag
    25501 gctgacgatg gcgcgaaacg tcagatagga aaagacgtta aagccggaat aatatttgac
    25561 caaatgttcg gccagccaaa ctaacatgtc ccattctcct gtaaagcgcg tactacctct
    25621 tccatggcgg cactacgtga acccttaact aaaatcgtaa ttacctgttg ctcagcaatc
    25681 agtgatttaa gacgcgtaat taacgcagtt ttatcagcaa aatgttcgcc aacgccgctg
    25741 gcggtgctga tagcatggct ttgtttaccc acgcttaaca cgcggtcaat accagcagct
    25801 tttgccgcct cgcccacctg tacatggcag gcttcgcttt cagcgcccag ttccgccata
    25861 tcgcccacca ccagcacgcg gtagcccggc atttcagcca gtacctgaac agcggcggtc
    25921 attgaaccga cattggcgtt gtaggagtcg tcgagcagca actggttttc tgccagttgg
    25981 atggggaaca gacggcctgg aacagctttc agatttgcca gccccgcttt gatagcatca
    26041 agcgttgcgc ccacggacat ggagagcgca gcggctgcca gcgcattcgc aatattgtga
    26101 cgccccggca acggcagcag aacatcgacg ctaccggttg gggtttgtag ggtaaattcc
    26161 gtaccgtgcg aggtcacatg gatattggtg gcggtgaaat cgctgttggc ggcattgggt
    26221 gagaaacgcc acactttgcg tgagccaatt acgctctgcc agttcagcca gtcgttgttg
    26281 tcggcgttca taatggcgat accgttttcc ggcaggccgc taaagatttc acctttcgct
    26341 ttcgcgacac ccgcaagcga gccaaaacct tccagatgcg ccgctgccag gttgttgacc
    26401 agcgcagctt ccgggcgagt cagactcaca gtccaggcta tttcgccctg atggttcgcg
    26461 ccaagttcaa taactgcgta atcgtattcc ggcgtcaagc gcaacaacgt catcggtaca
    26521 ccgatgtcgt tgttgagatt gcctgccgta taaagcgtgt tgccgcactg gcttaaaatc
    26581 gccgccgtca tctctttaac ggaggttttg ccggaggaac ctgtcagagc aaccacgcgc
    26641 gccggaactt gctggcgaac ccatgcagcc agttcaccaa acgccagacg cgtatccttg
    26701 acgattaact gcggcaggtc gatatccagc ggacggctga ccagtagtgc gcccgcgccg
    26761 ccagctttcg cctggtcggc aaaatcatga gcatcgaaac gttcgccttt cagggcaaca
    26821 aacaggcagc ccggcgtcag ttttcgggta tcagtggtta cagcatcaag ggtgatatct
    26881 gcaccttgca gttcaccgtt gagaatgtcg gtaagttggc taagggttac gctaatcatg
    26941 caatcacccc cagcagacgc gccaccgtga cgcgatcgga gtagtccaga cgctgattgc
    27001 caacaatctg gtaatcttca tggcctttgc ccgcgaccag taccacatca ttctctttag
    27061 cctgcataac ggcgcaagtc accgcttcag cacggccttc catcactttg gcatgtccgg
    27121 catctaacat tcccgccaga atatcgttga tgatggcacg cggttcttcg gtacgcgggt
    27181 tatcgtccgt caccaccgcc acgtcagcaa actcttcggc aattgcgccc atcagtggac
    27241 gcttaccttt atcgcgatcg ccaccacagc caaagacgca ccacagcttg cccgcacagt
    27301 gcaggcgcgc cgcctgtaag gctttttcca gtgcatccgg cgtatgcgcg taatccacca
    27361 ccaccgtcgg tttgcctggc gcagtgaaca cttccatacg tccgcaaacc ggttgcagac
    27421 gcgcggcggt tttcagcaga tcagccagtg gatagccgag tgccagcagt gtcgccagcg
    27481 cgagcagcag gttgctgacg ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc
    27541 cccaacttga gctaaagcga atcgtcgcac cgctgtcgtg atagttcacg tcgatcgctt
    27601 ttaaccagcg tccgtgacag ttcggattaa tatgatcttc cattgatacc gcaaccgcgt
    27661 ccggcagttt tgccagccag cggcggccca cttcatcgtc ggcgttaata atcgcctgac
    27721 cgcaatgatg ttcagaataa agcagccatt tcgcagcttc gtagtgttcc atatcaccat
    27781 gataatcaag gtgatcgcgg cttaagttgg taaagaccga cgccgcaaat ttcaatgccg
    27841 ccacacggtg ctgtaccagc ccgtgggagg aaacttccat tgcgcaaaac gtcgcgccct
    27901 gctccaccag ccccgccagc tcatgctgaa catcgactgc ggaaccggta gtattttctg
    27961 tcgggatcac tttcccaagc agaccgttac caacggtgcc cattaccgcg ctggtttcgc
    28021 caagcagttg gctccactgc gccagcagct gggtagtcgt ggttttgccg ttggtgcccg
    28081 ttacgcctac gagacgtaaa ttgtcagagg gttcatggta aaagcggccc gccagtgcag
    28141 ataaacgctc attgagctgg ctgagataga tgaccggtac gccgtgcatt tcacggattt
    28201 caccgtcggt cgcctcatct ttcgctttcg cctctgcaat aatggcagcc acaccttgcg
    28261 ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg acctactaca gctacaaaga
    28321 gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc tcgcagtgct cgcgaaggtg
    28381 cgtctggcac ccacggagca agaaggtcgc gcaaattacg atctgccacc tgtcccctcg
    28441 ccttgattaa tcacaaattc atttttatcg cccgttgtca gcgcatccgg ctcgatgttc
    28501 atggtacgca atacgccgcc catgatggca ccaaagaccg gcgcggaaac ggcgccgccg
    28561 tagtatttac ccgcctgcgg atcgttgata acaacaacca gcgcgaagcg cggctgactc
    28621 gcaggcgcaa cgcctgcggt ataagcaata tatttattga tgtagcgacc gtccggcccg
    28681 acctttttcg cggtaccggt tttaatggcg atacgatagc ctttaatcgc cgccttcacg
    28741 ccgccgccgc ctggtagcgc cacgctttcc atcatatgca ccacagtgcg gacaatggat
    28801 cccgggaaga cacgttcacc gggaaccggg gggtcaactt tggtaatcga cagtgggcga
    28861 taaatgccgt agctgccgat agttgcgtag actcgcgcta actgtaacgg tgttaccatt
    28921 agcccgtagc cgaaagagaa ggtggccctc tctatgtcag accaccgttg tttttgagga
    28981 tataagccac tgcgttctcc gaccaacccc aaattggtcg cttttcccag tccaaaacgt
    29041 gagtaagtat ctactaacgc tgaggacggc atcgctaacg ccagcttgga aacaccgacg
    29101 ttactcgact tctgtaatac cccggtcagg gttaattcgc tgtagcgtgc cacgtctttg
    29161 atttcgtggc cgttaattcg ataaggaatg gtattgagta ccgagttttc ccgcaccacg
    29221 ccacgttgca acgcggtcat taccaccatc ggtttaaccg ttgagcccgg ttcaaacacg
    29281 tcggtgatgg tacggttacg catcgcctct ttcggcgtgc cgctcagatt gttagggttg
    29341 tatgacgggc tgttagccat cgccagcact tcaccggtgt tgacatccac cagcacggcg
    29401 ctaccagatt cagccttgtt aaaggccacc gcgttgttca gttcgcgata aaccagcgcc
    29461 tgcaggcgtt catcaatact cagcgccagg ttgtgcgctg cctggctgtc agtagaagaa
    29521 atatcttcaa ttacgcgacc atagcggtct ttacgcacaa tgcgctcacc cggctgcccg
    29581 gtaagccatt tatcgaaact cttctcaacg ccctcaatcc cttgactatc gacgttagta
    29641 aagccgatga ggtgagcagt cacttcgccg gacggatagt aacggcgaga ctcttcacgc
    29701 agatgaatcc ccggcagttt cagttttttg atgtagtccg ccatgtcagg gttcacctga
    29761 cgcgccagat aaataaaacg ccctttcggg ttggcgttaa tgcgggctga aagctgatcc
    29821 agcggaatat tgagcgcgtt agccagcgcc ttccagcggt caccgacgct gataccgcca
    29881 gcgtcatgca cttctttcgg gtcagcccaa atcgctttta ccggcacgct caccgctaac
    29941 gggcgaccag aacggtcagt aatcatgccg cgggaggtgg aaacttgctg aacgcgaaga
    30001 gaacgcatgt cgccctcttt caccagcata tccggggaga taacttgtaa ccacgctacg
    30061 cgtccgagca gaaaagccag cgccaggaga atacagccgc ataacaacgc aaaacgccaa
    30121 ctgataaagt tggcatgttc ttcctgacgt tttggtttct gcgttttcgc cgctgctttc
    30181 atgcgtcgcg tttatcctta tttttgcact acgatatttt cttgtgacgg atcaacatgc
    30241 tgcatttgca gcttttccgt ggcgatcctt tccacccggc tatggtcgcc gagcgcattc
    30301 tcttcaagga tcaggttgcg ccattcaatg tctaaagcat ctcgctccag caccagttgt
    30361 tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg ttaccacagt caccgccgtc
    30421 aaaataatgc aaatgaacag gcagagtggc agcttcccaa atcgcaaaag atcgtcaccg
    30481 ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg atcctttaac tttgcttaga
    30541 gcttctgtca ctctgctgat catgcattcg tcctctctgc aatacgcaga actgaactac
    30601 gggcacgagg gttctcagcc acctcttctt cgcccggcat taacttgcct agtgctcgca
    30661 gctgacggcc acccagtttt ttgagctgct cttcagtcat cggtaaccct gccggaactt
    30721 gcggaccgcg gctgttttca cgcataaaac gtttcacaat acggtcttcc agcgagtgga
    30781 agctgatgat cgaaagccgc ccacccgggg ccagcacgtt gagcgagctt tttagcgcct
    30841 gctctatctc ctccagttca ctgtttaccc aaatgcgcac cgcctggaag gtacgggtcg
    30901 cgggatgttt aaacttatct ttcaccggcg ttgcaacagc cacgacttcc gccagttctt
    30961 tggtgcgggt catcggctgt tcgcggttac gctcgacaat ggcgcgggca atgcgtttgg
    31021 caaaacgctc ttcaccatag gttttcaata cccaagcgat atcggcttct tctgcggttt
    31081 gtagccattc agcggctgac tgcccacggg ttgggtccat acgcatgtcc agcggaccat
    31141 cgcgcataaa ggaaaagccg cgctcagcat catcaagttg cggtgaagag acgccaagat
    31201 cgaggagaat gccgtcgatc ttgccgataa gatcgcgctc ggcaacgtat tcgcccagcg
    31261 cggagaaagg tccgtggatg atggagaagc gcggatcatc aatagtcttc gcaacggcga
    31321 tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc ttcgccaagc tgcgagagga
    31381 tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat gtagatgcca tcaggacgga
    31441 tattgaggcc attaacggct tcatccagca gcaccgtagt atgtttatag ttttccatca
    31501 ttttatagag acaagtcctg cagtcgctcc gataagtctc cggtagccaa ctgctctgcg
    31561 tcgatatctt ccttgacctg ttgatgccag gttgtttcat cccacagctc aaacttgttg
    31621 aactgtccaa ccagcatcac ttcttttgtc agcccggcat gttgccgcag tactggcgcg
    31681 attaacaatc gacctgcgcc atccatctga cattcgctgg catgacctaa cagtaggcgc
    31741 tgcacacggc gctcaaccgg gttcatgctc gacagacgcg ataatttttg ctcgataatt
    31801 tcccattcag gcagggggta aagcagcagg cacgggtgat gaatgtcaat ggtgcaaacc
    31861 atttgaccga cagcgttctc aagcagctgt tcccggtaac gggtaggcac tgataagcgc
    31921 cctttgctgt cgagattgac taacgttgct ccccggaaca tgccagcctc accccttatt
    31981 cccactttgc cccacaattt cccactgaaa ggagtttacg gagcggagga aaagcttgtc
    32041 aagccagtca caaggcatac cgaggtaaaa aacccgatgt ttacggctaa tatcagcgtt
    32101 gtttatttta cgtccaacgt gcgcagcaaa ttaacgttat gaatagctgt aagaaaaaat
    32161 ccctcatcgt catcaccgtt ttaaaatgac tcactatttt ccacagcaaa tataaagtgt
    32221 cagtttgcga cgcgagcggc attttaggac atatcttccc cggttaacag tccctgttgc
    32281 gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc aaagaaaagt gcttgttaaa
    32341 tcggcaattc atctctgcat gtaacaaaat aatgcaaaaa cgcgctgtcg atatcactta
    32401 attattgtga atttcagctg gtgagttaat taacgggcgg gtttaaaaga acttatcaat
    32461 aatagtcagg aatagtctta tttactttaa ggatattgat gtccattccc gtactctacg
//
LOCUS       DAZOZD010000021        32380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOZD010000021 DAZOZD010000000
VERSION     DAZOZD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.021"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            139..702
                     /locus_tag="ACHUPB_000792"
     CDS             139..702
                     /locus_tag="ACHUPB_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0757686.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            784..3426
                     /gene="yfcU"
                     /locus_tag="ACHUPB_000793"
     CDS             784..3426
                     /gene="yfcU"
                     /locus_tag="ACHUPB_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR0757687.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3446..4197
                     /locus_tag="ACHUPB_000794"
                     /pseudo
     CDS             3446..4197
                     /locus_tag="ACHUPB_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4172..4720
                     /locus_tag="ACHUPB_000795"
     CDS             4172..4720
                     /locus_tag="ACHUPB_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0757688.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4717..5187
                     /locus_tag="ACHUPB_000796"
     CDS             4717..5187
                     /locus_tag="ACHUPB_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0757689.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5184..5708
                     /locus_tag="ACHUPB_000797"
     CDS             5184..5708
                     /locus_tag="ACHUPB_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0757690.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5695..6567
                     /locus_tag="ACHUPB_000798"
     CDS             5695..6567
                     /locus_tag="ACHUPB_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0757691.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6689..7240)
                     /gene="smrB"
                     /locus_tag="ACHUPB_000799"
     CDS             complement(6689..7240)
                     /gene="smrB"
                     /locus_tag="ACHUPB_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0757692.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7406..8338
                     /gene="prmB"
                     /locus_tag="ACHUPB_000800"
     CDS             7406..8338
                     /gene="prmB"
                     /locus_tag="ACHUPB_000800"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0757693.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8373..9458
                     /gene="aroC"
                     /locus_tag="ACHUPB_000801"
     CDS             8373..9458
                     /gene="aroC"
                     /locus_tag="ACHUPB_000801"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0757694.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9462..10286
                     /gene="mepA"
                     /locus_tag="ACHUPB_000802"
     CDS             9462..10286
                     /gene="mepA"
                     /locus_tag="ACHUPB_000802"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0757695.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10286..11095
                     /locus_tag="ACHUPB_000803"
     CDS             10286..11095
                     /locus_tag="ACHUPB_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR0757696.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11095..11643
                     /gene="epmC"
                     /locus_tag="ACHUPB_000804"
     CDS             11095..11643
                     /gene="epmC"
                     /locus_tag="ACHUPB_000804"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0757697.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11677..11955
                     /locus_tag="ACHUPB_000805"
     CDS             11677..11955
                     /locus_tag="ACHUPB_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0757698.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12076..14082)
                     /gene="mnmC"
                     /locus_tag="ACHUPB_000806"
     CDS             complement(12076..14082)
                     /gene="mnmC"
                     /locus_tag="ACHUPB_000806"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0757699.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14241..15461
                     /gene="fabB"
                     /locus_tag="ACHUPB_000807"
     CDS             14241..15461
                     /gene="fabB"
                     /locus_tag="ACHUPB_000807"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0757700.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15736..16914
                     /locus_tag="ACHUPB_000808"
     CDS             15736..16914
                     /locus_tag="ACHUPB_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0757701.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16911..17906)
                     /gene="flk"
                     /locus_tag="ACHUPB_000809"
     CDS             complement(16911..17906)
                     /gene="flk"
                     /locus_tag="ACHUPB_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0757702.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18005..19141
                     /gene="pdxB"
                     /locus_tag="ACHUPB_000810"
     CDS             18005..19141
                     /gene="pdxB"
                     /locus_tag="ACHUPB_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0757703.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19207..20220
                     /locus_tag="ACHUPB_000811"
     CDS             19207..20220
                     /locus_tag="ACHUPB_000811"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0757704.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20220..21032
                     /gene="truA"
                     /locus_tag="ACHUPB_000812"
     CDS             20220..21032
                     /gene="truA"
                     /locus_tag="ACHUPB_000812"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0757705.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21115..21774
                     /locus_tag="ACHUPB_000813"
     CDS             21115..21774
                     /locus_tag="ACHUPB_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0757706.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            21930..22844
                     /gene="accD"
                     /locus_tag="ACHUPB_000814"
     CDS             21930..22844
                     /gene="accD"
                     /locus_tag="ACHUPB_000814"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0757707.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22914..24182
                     /gene="folC"
                     /locus_tag="ACHUPB_000815"
     CDS             22914..24182
                     /gene="folC"
                     /locus_tag="ACHUPB_000815"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0757708.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24172..24834
                     /gene="dedD"
                     /locus_tag="ACHUPB_000816"
     CDS             24172..24834
                     /gene="dedD"
                     /locus_tag="ACHUPB_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0757709.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25093..25581
                     /gene="cvpA"
                     /locus_tag="ACHUPB_000817"
     CDS             25093..25581
                     /gene="cvpA"
                     /locus_tag="ACHUPB_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0757710.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25618..27135
                     /gene="purF"
                     /locus_tag="ACHUPB_000818"
     CDS             25618..27135
                     /gene="purF"
                     /locus_tag="ACHUPB_000818"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0757711.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27230..27799
                     /gene="ubiX"
                     /locus_tag="ACHUPB_000819"
     CDS             27230..27799
                     /gene="ubiX"
                     /locus_tag="ACHUPB_000819"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0757712.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28065..28847
                     /gene="argT"
                     /locus_tag="ACHUPB_000820"
     CDS             28065..28847
                     /gene="argT"
                     /locus_tag="ACHUPB_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0757713.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            29068..29850
                     /gene="hisJ"
                     /locus_tag="ACHUPB_000821"
     CDS             29068..29850
                     /gene="hisJ"
                     /locus_tag="ACHUPB_000821"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0757714.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            29940..30626
                     /gene="hisQ"
                     /locus_tag="ACHUPB_000822"
     CDS             29940..30626
                     /gene="hisQ"
                     /locus_tag="ACHUPB_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0757715.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30623..31339
                     /locus_tag="ACHUPB_000823"
     CDS             30623..31339
                     /locus_tag="ACHUPB_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0757716.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31347..32120
                     /gene="hisP"
                     /locus_tag="ACHUPB_000824"
     CDS             31347..32120
                     /gene="hisP"
                     /locus_tag="ACHUPB_000824"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0757717.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32317..>32380
                     /locus_tag="ACHUPB_000825"
     CDS             32317..>32380
                     /locus_tag="ACHUPB_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757718.1"
                     /translation="MTISTTSTPHDAVFKSFLRHP"
BASE COUNT         7593 a         8008 c         8749 g         8030 t
ORIGIN      
        1 aacgaataca attttctacg acctgttctt tttggatcgc atgccgtatt caatggctgg
       61 gtataaacat tggttattga ctgaatcaat gtattgcaat gtttatattt ttaattattt
      121 atagtttaag gacaaataat gagtaagttt gttaaaacag ctattgctgc ggcaatggtg
      181 atgggcgtgt tcacctctac ggcaacaatc gctgcgggta acaacggtac tgcacgtttt
      241 tacggcacca ttgaagactc agtgtgttct atcgttccgg acgatcacaa actggaagtt
      301 gatatgggtg atatcggtgc cgaaaaactg aaaaataacg gcaccaccac gccgaaaagt
      361 ttccagattc gtctgcaaga ttgcgtattt gatactcagg aaacaatgac cactaccttt
      421 actggtaccg tttcttctgc aaatagcggc aattattaca ccattttcaa taccgatacc
      481 tgtgcggcat ttaacaatgt tagcctggcg atcggtgact ctctgggtac ctcttacaaa
      541 agcggcatgg gtattgacca gaaaatagtg aaagacactt ctaccaataa aggcaaagcg
      601 aagcagacac tgaactttaa agcctggctg gtcggcgcag ctgatgcgcc agatctgggt
      661 aatttcgaag caaataccac cttccagatt acttacctgt aaggcatacc cccatagcga
      721 cactgctatg ggggatttaa atggattttg accgaacaag aataaaaacg gatagaaacg
      781 tgtatgcctg accattctct ttttcgatta cggattcttc cgtggtgcat tgcgctggca
      841 atgtcaggga gttatagcag tgtctgggct gaagacgaca ttcagtttga ttcccgtttt
      901 ctggaattaa aaggcgacac aaaaattgat ctgaagcgtt tttccagcca gggatatgtt
      961 gagcccggaa aatacaattt acaggttcaa ctaaataaac agccattggc ggaagagtac
     1021 gatatttact ggtatgctgg tgaagatgac gcgagcaaaa gctatgcttg tctgacaccg
     1081 gaactggtag cgcagtttgg tttaaaagaa gacgtggcga aaaatctgca atggagccac
     1141 gatgctaaat gcctgaaatc cggtcaactg gaaggcatgg aaattaaggc tgatttaagc
     1201 cagtccgcat tagtcatttc actgccacag gcttacctcg aatatactta tcccgactgg
     1261 gatccgcctt cacgttggga tgacggcatc tccgggatcg tcgcggacta cagcatcaac
     1321 gcacaaaccc ggcacgaaga aaatggcggt gatgatagta acgagatcag cggcaacggg
     1381 acggtcgggg ttaacctggg gccgtggcgt atgcgtgctg actggcagac taactatcaa
     1441 catactcgca gtaatgatga cgatgaattc agcggcgatg aaactcaaaa aaaatgggag
     1501 tggagtcgct actatgcctg gcgggcgtta ccatcattaa aagccaaact ggcgctgggc
     1561 gaggattacc tcagatccga tatttttgat ggttttaact atgttggtgg cagtgtcagt
     1621 actgacgatc aaatgttgcc tcccaatctg cgcggctacg cgccagacat ttccggcgtg
     1681 gcacacacca cagcaaaagt gaccgtcagc cagatggggc gtgtgattta cgaaacgcag
     1741 gttccggctg gaccgtttcg tattcaggat cttggtgatt ctgtctccgg tacgttgcat
     1801 attcgcattg aagaacagaa cggccaggtg caggaatatg acatcagcac cgcctcgatg
     1861 ccatacctca ctcgtccagg tcaggttcgc tataagatca tgatgggccg tccgcaagag
     1921 tggggacacc atgtcgaggg tgaatttttt tctggtgctg aagcttcctg ggggatcgct
     1981 aacggctggt cgttatatgg cggcgcactg ggagatgaaa actatcagtc tgcggcgctt
     2041 ggcgtcggtc gcgatttgtc tacattcggc gcggtcgcgt ttgatgttac tcactcgcac
     2101 accaaactgg ataaagacac cgcttatggc aaaggttcgc tggacggtaa ctccttccgt
     2161 gtgagttatt ccaaagactt tgaccagctc aacagccgcg ttacttttgc tggatatcgc
     2221 ttctcggaag agaactttat gaccatgagc gagtatctgg atgccagtga cagcggaatg
     2281 gtacgcacgg gcaacgacaa agagatgtac actgccactt ataaccagaa cttccgcgat
     2341 gcgggtgttt cggtttatct caactatacc cgccatacct actgggatcg cgaggagcag
     2401 acaaactaca acatcatgct ctcgcactat ttcaatatag gcagtattcg taatgtcagc
     2461 atctcgatga ctggctaccg ttacgagtat gacaaccagg ccgacaaagg catgtacatt
     2521 tcgctcagta tgccgtgggg tgacaacagt accgttagct ataacggtaa ctatggcagt
     2581 gggacggaca gcagtcaggt cggttatttc agccgtgtcg atgacgcgac tcactatcag
     2641 ttgaacgtcg gcaccagtga caaacacacc agcgttgacg gctattacag ccatgatggt
     2701 tcgctggcgc aggttgacct cagtgcgaac taccatgaag ggcaatacac ctctgcgggc
     2761 ttgtcgttac agggcggcgc aacgcttact gcccacggtg gcgcacttca ccgtacccag
     2821 aatatgggcg ggacacgctt gttgattgat gccgatggcg ttgccgatgt tccggtagaa
     2881 ggtaacggtg ctgctgttta taccaatatg tttggtaaag ccgtcgtttc tgacgtcaat
     2941 aactattacc gcaatcaggc gtatatcgac ctcaacaaac tgccggaaaa cgccgaagca
     3001 acccagtcgg tggtacaagc cacgctaact gaaggggcga ttggctaccg caaatttgcc
     3061 gtcatcagtg gtcaaaaagc gatggcggtg ctgcgtttac aagacggcag ccatcctccg
     3121 tttggcgcag aagtaaaaaa tgataacgag cagacagtgg gccttgtcga tgatgacggc
     3181 aatgtttatc tggctggggt gaaacctggc gaacacatga gtgtgttctg gagtggtgtt
     3241 gcgcattgcg atatcaacct gccggacccg ctgcctgccg atctgtttaa cggcttgtta
     3301 ctgccatgcc agcataaagg caacgtcgcg ccggttgttc ctgatgacat aaagcctgtc
     3361 attcaggagc agacgcaaca ggtgacaccc accaatcccc ctgtttctgt ttcagctaac
     3421 caataacgtg attaaggaat gatccatgtc tgatttactt tgctctgcca aattgggtgc
     3481 tacgacgcta gcgttactgc ttagcgctgc cagtttgtcg cccaggcttc tgtaacgcca
     3541 gatcgtaccc gtttgatatt taatgaaagc gacaaatcaa tcagcgtgac gttgcgcaat
     3601 aacgatccga aactgcctta tctggcccaa agctggatcg aagatgaaaa gggtaacaag
     3661 atcagttcac cgttaactgt tctgccaccg gtgcaacgca tcgattcaat gatgaacggg
     3721 caggtaaaag tgcagggaat gccggatatc aacaaattgc ctgccgatcg tgaaagcctg
     3781 ttctacttca acgtgcgtga gatcccgcca aaatcaaaca aagccaacac gctgcaaata
     3841 gcactacaaa cgcgtattaa gttgttctgg cgaccgaagg cgctggagaa cgtcagcatg
     3901 aaaaacccct ggcaatataa ggttacttta acccgcaacg gtcaggaatt taccgtcaat
     3961 aacccgacgc cgtattacgt cattttcagt aatgccagca cacagaaaaa tggcaatcct
     4021 gcagccggtt tctcgccaat agtgatgtcg ccgaaaacct ctgcaccgct gaatgtgaag
     4081 atgggtagcg tgccggtact gacctatgtc aacgactatg gcgctcggat gccgctcttt
     4141 ttcacttgca acggtaacag ttgccaggtc gatgaagagc agagccgcaa aggctgaggc
     4201 cgggagagcc agtatgaaag gatcttgttc tgtctgggcg gtaactgggt tactgatatg
     4261 ttgtgttggt agcggctttg cagttgcagc gagtaagtca gtaagtatga atttacgggt
     4321 gcttgtcgat gcccctcctc cttgtacggt aaatggtgcg gcagttgagt ttggtaatgt
     4381 attcattaat aagatcaatg gggttgatta taaacgtccc attgactact cgcttgtttg
     4441 taacaatctg gcaatggatg acctgcgtct acagatgcag gcgacaacgg tcgtgattaa
     4501 cggtgaaaca gttatcagca cgggtattcc tgggtttggt attcgggttc agaaaagtag
     4561 tgatcacaca atactcgact taacctctgg ctcatggttg ccatttaatt tttcctcggg
     4621 agttcccgtt ctggaagctg tgccggtgaa acagagtggt acaacgttag cggcagctga
     4681 atttaacgcc agtgccacaa ttgtggtgga ttaccaatga aacggatctc gcttattttg
     4741 ctgtggggct tttgctcgat ggcactctca aacgtctctt ttcacggtta tttagtgcag
     4801 ccgcctaact gcacgattag caacgctcaa accattgaaa taacttttca ggatgtgctt
     4861 attgatgaca ttaatggcag caattatgag caaaccgtgc cgtattccat tacctgcgat
     4921 acggcggttc gcgacccatt aatggaaatg accttgagct ggagtggcac actaagcgat
     4981 tttgataatg ctgcagtaag cagcaatatc actggtttag gcattcagct caaacaggca
     5041 gggcagagtt ttacgatcaa tacgccactt atggtgaatg aaaccgattt accggtgtta
     5101 acggctgtgc cagtaaaaaa gagtggtgtc gttctgccag aggcggactt tgaggcctgg
     5161 gccacgttac aggtagatta ccaatgaaga aaaagcgtac gttgtttttt attagtagcc
     5221 tgatgttgct gggcagtgga accacaatag caggagataa tttgcacttt accggcaatt
     5281 taatcagtaa atcgtgtacg ccagtgatta atggtagcca gttggcagaa gtacattttc
     5341 ctgccattgc cgccagcgat ttaatgaacc ttgggcagtc agagcgagtg ccattggtgt
     5401 ttcaattgaa agattgtcat agttctacat tgtttaatgt caaagtaacg ttgacaggaa
     5461 cagaagatag tgctctaccc ggctttctgg cctttgactc ctcgtcatca gcatccggcg
     5521 ctgggattgg tattgaaact gcggcaggca catcggtgcc aatcaacaat acaacaggcg
     5581 tcacgctccc gctgaatcag ggaaataaca gcctaaattt taatacctgg ttacaggcaa
     5641 agagcgggcg agatgtaacg tccggagact ttagtgccac agtgacggca acctttgagt
     5701 atttctgaaa tgaaaaaatg gaccataatt ttcgcgagcc taatgctgct cgttctgagt
     5761 gttgtcggcg catctaagag ttatgctgga gataaactta tgagtgcgag ttttgacagt
     5821 gtgagagttt attatgccat ggacaaggta accggtgctg tagcgagcag tgttttcaat
     5881 gttacggtga taacgccaaa ggaggttaca tatgggaaat atgattcttt tgcgtataaa
     5941 ggcgatacgt tgcgggttat ttcctggtct gggtcgggtt cagctccaac tttggtactg
     6001 actgactttg atacaattaa taactcaaat tgccctggca tagatacgaa aatttttcgt
     6061 tgtgcatata tgacgtttaa aattaccgtt gcatctgatg attatggttg cccttggatc
     6121 gcatcctttt attcctatac agatttaccc ggatttggaa gctatacggc ccctacagtg
     6181 cataacacaa tatgcccaac gataccggtc acttcatacg atatctcctg gagtgaaaat
     6241 tatgtcagcc ataataaggc gttacgaatt cagtccacgg ggtcaacagt gaccaccacg
     6301 ctttcgacct acctgatgga aggcggcaga ctctgtgatg gcagtaattt ttctgataat
     6361 gatggcagag gagcatactg ccgcgcggta tcagaattgt taacctttac ctcttacggt
     6421 tgtgataaat caacggtaac ggttacgcca actcgtcatc cggtaacgga taaagtacta
     6481 cacgatattg tggtgaatgt gaataccagt agtgggcaac cgattgattc gacgtgtcgt
     6541 tttcagtatg tattgaatga actgtagttc gtagtattgc tctgatgcgt cttatcaggc
     6601 ctacgaagtg aattgcaatt tattgaatta tcacatattt gtaggccgga taaggcgttt
     6661 acgctgctcc ggcatttgct gccagaattc agggcaactc cggcggcaac cactcttcca
     6721 cttcaatcaa caccaacaaa gccgcatcac cgccgtactc tttcggtgcc tggtgaaatg
     6781 ccatcacatg cggatgttgt gccagccaca gcggtgtttg ttgcttcaaa atatgcttcc
     6841 cgtgcccatg catcacgcag gcacaaaaca catgttcgcg gcgacaggcg gcaatcaacg
     6901 cgcccagttc ctgcttggcc tgcagctgcg tcagaccgtg taaatccaaa aacaattccg
     6961 gtgaataatc gccgcggcgc agtttcttcg cctcaaaatg gctgacatcc gggcgaacat
     7021 atttcaccgg accttcggta tttaataacg gctgaaactc atcggagaaa taatggctgg
     7081 catcagcctg ctccgggatc aagcgtttca ccggcacttc gctgattttt ttacgctgcg
     7141 gtcggtggac aatcgtgtcc tgcttaatct tgcgagtccc cgccatcaac tggcgaaaca
     7201 gagcctggtc ctcctcgcta agtgttgttt tctttttcat ttatctgtct ctatttctaa
     7261 ttttcctaca gtttacccga ctcgtagcac aatcgatcgt tttaaacgta tttttcctca
     7321 ctcaccgcgc gtgaatgctg atttatcgtc atcttcatgg caaactagcc gccgaaatta
     7381 ttgcgatcat gccctggagg aatacgtgga taaaattttc gttgatgaag cagtaaatga
     7441 gctgcaaacc attcaggaca tgttgcgctg gtcggtaagc cgcttcagcg cggcaaatat
     7501 ctggtacggt cacggcaccg ataacccgtg ggatgaagcc gtacagctgg tgttaccatc
     7561 gctctacctg ccgctggata tcccggaaga tatgcgcacc gcgcgtctga cctccagcga
     7621 aaaacaccgt attgttgaac gcgtgatccg ccgcgtcaac gagcgcattc cggtggctta
     7681 cctgaccaac aaagcctggt tctgcggtca tgaattttac gtcgatgaac gcgtgctggt
     7741 gccgcgctcg ccgattggtg aactgatcaa caataaattt gccggactca ttagcaagca
     7801 accgcagcat attttagata tgtgtaccgg tagcggctgc atcgccattg cctgtgctta
     7861 tgccttcccg gaagcggaag tcgacgcggt agatatctct cccgacgcgc tggcggttgc
     7921 cgaacagaac atcgaagaac acggtctgat ccacaacgtc attccgattc gttccgatct
     7981 gttccgtgat ttgccgaaag tgcagtacga tctgattgtc actaacccgc cgtatgtcga
     8041 cgcggaagat atgtccgacc tgccaaacga ataccgtcac gagccggaac tgggcctggc
     8101 atctggcacc gacggcctta aactgacgcg tcgcattctt ggaaacgcgg cagattacct
     8161 tgctgatgat ggcgtgttga tttgtgaagt cggcaacagc atggtacatc ttatggaaca
     8221 atatccggat gttccgttca cctggctgga gtttgataac ggcggcgatg gtgtgtttat
     8281 gctcaccaaa gagcagctgc tggcggcccg tgaatatttc gcgatttata aagattaagt
     8341 aaacacgcaa acacaacaat aacggagccg tgatggctgg aaacacaatt ggacaactct
     8401 ttcgcgtaac caccttcggc gaatcgcatg ggctggcgct cggctgcatc gtcgatggtg
     8461 ttccgccagg cattccgctg acggaagcgg acctgcaaca tgacctcgac cgtcgtcgcc
     8521 ctgggacatc gcgctatacc acccagcgcc gcgagccgga tcaggtcaaa attctctccg
     8581 gtgtttttga aggcgttact actggcacca gcattggctt gttgatcgaa aacactgacc
     8641 agcgctctca ggattacagc gcaattaaag acgttttccg cccaggccat gctgattaca
     8701 cctacgaaca aaaatacggt ctgcgcgatt atcgcggcgg tggacgttct tccgcccgcg
     8761 aaaccgccat gcgcgtggcg gcaggggcga ttgccaaaaa atatctcgcc gagaaatttg
     8821 gtattgaaat ccgcggctgc ttgacccaga tgggcgacat tccgctggaa atcaaagact
     8881 ggtcgctggt tgaacaaaat ccgttcttct gcccggaccc ggacaaaatc gacgcgttag
     8941 atgaactgat gcgcgcgctg aaaaaagagg gcgactccat cggcgcgaaa gtcaccgttg
     9001 ttgccagtgg tgtccccgcc ggacttggcg agccggtctt tgaccgcctg gatgccgaca
     9061 tcgcccatgc gctgatgagc atcaacgcgg tgaaaggcgt ggaaattggc gacggttttg
     9121 acgtagtggc gctgcgcggc agccagaacc gcgacgaaat caccaaagac ggtttccaga
     9181 gcaaccatgc gggcggcatt ctcggcggta tcagcagcgg gcagcaaatc attgctcata
     9241 tggcgctgaa accgacctcc agcattaccg tgccggggcg cactattaac cgctttggcg
     9301 aagaagttga gatgatcacc aaaggccgtc acgatccctg tgtcgggatc cgtgcagtgc
     9361 cgatcgctga agcgatgctg gcgatcgttt taatggatca cctgttacgg cagcgggcgc
     9421 aaaatgccga tgtgaagact gatattccac gctggtaaaa aatgaacaaa accgcgattg
     9481 cgctgctggc tctgcttgcc agtagcgcta gcctggcagc gacgccgtgg caaaaaataa
     9541 cccaacctgt gccgggtagc gcacaatcga taggcagttt ttctaatggc tgtattgtcg
     9601 gcgctgacac gttgccgata cagtccgaac attatcaggt catgcgtacc gatcagcgtc
     9661 gctatttcgg tcacccggat ctggtgatgt ttatccagcg tctgagtagc caggtgagca
     9721 atctgggcat gggtacggtg ctgattggcg atatggggat gcccgctggt gggcgtttca
     9781 acggcggtca tgccagccac cagaccggac tggatgtcga tatctttctg caactgccga
     9841 aaactcgctg gacctccgcg cagctcttgc gcccgcaagc actggactta gtttcccgcg
     9901 acggtaaaca cgttgtctcc acgctgtgga agccagaaat tttcagcttg atcaaactcg
     9961 ctgcccagga caaagacgtc acgcgcattt ttgttaatcc ggcgattaaa caacaacttt
    10021 gccttgatgc gggcaccgat cgcgactggt tgcgcaaagt gcgaccctgg ttccagcatc
    10081 gcgcgcatat gcatgtacga ttacgttgcc ctgccgatag tctggagtgt gaagatcaac
    10141 ctttaccgcc atcaggcgat ggttgcgggg cagaactgca aagctggttt gcacctctaa
    10201 aaccgggaac aacaaagcct gagaagaaga caccgcctcc gttgccgcct tcctgccagg
    10261 cgctactgga tgagcacgtg atctaatgga aacgtttaat agcctgttta tggtttcccc
    10321 gctgttgctg ggagttctct tttttgtcgc catgctggcg ggatttatcg actcgattgc
    10381 cggtggcggt gggttactca ccattccggc attgatggct gcgggaatgt ctcccgctaa
    10441 cgcgctggca accaataaac tgcaagcctg cggcggctct atttccgcca ctatctactt
    10501 tattcgccgc aaagtggtta gtttaagcga tcagaaactc aatattgcca tgacctttgt
    10561 cggctcaatg agcggcgcac tgttggtaca gtacgttcag gctgatgtct tacggcagat
    10621 tttgcccatt ctggtgattt gtatcggcct ctatttcttg ctgatgccaa agctgggtga
    10681 agaagatcgc cagcgccgga tgtacggttt acccttcgcg ttaattgccg gtggctgcgt
    10741 cggtttttat gacggattct ttggcccggc tgccggatcg ttttacgcgc tggccttcgt
    10801 tacgctgtgc ggattcaacc tcgccaaagc cacggctcac gccaaattac tcaacgcaac
    10861 gtcaaacatt ggcggtctgc tgctgtttat tctcggcggc aaagtgattt gggcgacggg
    10921 ctttgtcatg ctggtggggc agttcctcgg cgcgcgcatg ggttcacggc tggtgttgag
    10981 caaaggacaa aagctgatcc gcccgatgat tgttatcgtc tcggcggtga tgagtgccaa
    11041 attactttac gatagccacg gacaggagat cctccactgg ttggggatga actaatgaac
    11101 agtacacacc actatgagca gttgattgaa atttttaata gctgctttgc cgatgaattt
    11161 aatacccgtc tgattaaagg cgacgacgaa ccgatctatc ttcctgctga tgcggaagta
    11221 ccgtataacc gaattgtctt tgcccacggc ttttatgcca gcgcaattca tgagatttcg
    11281 cactggtgta ttgccggaaa agcgcgccgt gagctggttg atttcggcta ctggtattgc
    11341 ccggatgggc gtgatgccca aacgcaaagc cagtttgaag atgttgaagt gaagccgcag
    11401 gcactcgact ggttgttctg tgtggcggcg ggatatccgt ttaacgtcag ttgcgacaat
    11461 ctggaaggcg acttcgaacc ggatcgtgtt gtgtttcaac ggcgggtaca tgcgcaggtg
    11521 atggattatc tgacaaatgg cattcccgag cgtccggcac gcttcattaa agcactacaa
    11581 aattattatc acacgccgga acttacggcg gaacagttcc cgtggccgga agcgctcaac
    11641 tgaagccggt gccacgtttt acaatgagga aagaagatga tcgcggagtt tgaatcacgc
    11701 attctggcat taatcgacgg catggttgac catgccagtg atgatgagtt gtttgccagt
    11761 gggtatttgc gtggccacct gacattagcc atcgcagaac tggaaagtgg tgatgaccac
    11821 tccgctcagg cggtgcatac gaccgttagc cagagtctgg aaaaagccat tggtgccggt
    11881 gaattgtcgc cgcgtgacca ggcgctggtg accgatatgt gggaaaacct gtttcagcag
    11941 gcgtcacagc agtaagttgc ggcgtctgga gcaccatgcc tgatgcgacg cttatcgcgt
    12001 cttatcaggc ctacacgtca cacgtaggtc ggataaggcg ttcacgccgc atccggcaaa
    12061 cactgcgaga gcagattacc ccgccttaac cgctttaccc ttcaacaatt tccgcaccca
    12121 taaccgattc gggtttaacg ccgtcagcgt actggcatcc atcggaatcg gttcgtcgct
    12181 catctgcgcc gccagaatct cggcacacag cggggcagaa cacaaaccgc gagaacctaa
    12241 agccgcaaac ataaagagat cgtcaaaaac cggcgcgctt accgcctcat ctttctgctc
    12301 cgccaacgac gcatattcca cgagtgttgc ctcataatcg ggaacattgc ctaccattgg
    12361 cagatgatcg cgggtggcac aacgcacacc gcagcgcgcc tctttatcac tgacatcaac
    12421 ctcttttgcc cactgtgcct gcgggaaaca atcaatcaac cgctggcgat tctgctgctg
    12481 atcgtcctca ctgtacgccg tatcttcgct gccgcgatga taactggcac caatacaatg
    12541 atgttgattc gccggatttt gtggcgtgag ataaccgtca tagcacagca cctgcttcag
    12601 ctctgccagt tccggcgttg tcggaatatg gctgacctgc ccggcaaccg aatacaccgg
    12661 gagagtcgac gtttggctga aacggctgat ttgatgcccg ttcgccagta ccactacgct
    12721 gtgtgttgct tgctgatctc ctgcaaaatt caacaaccaa cagtcatcct tacgggataa
    12781 attctgtaac tgatattgat aataaatctg caaaccctgc tgttgcgcca gttccagcac
    12841 attacgggtc agttctgctg ggcacagcca accaccttgc ggataagtaa tgccgctgca
    12901 atttgtcgca acgcccgtaa tttgttcaac cgcatttgcc tcaacggcta cagccagttc
    12961 tgcgggtaaa tccattgaca acatctgtgc gattttatgc tggcttttct catcccagcc
    13021 taactgcgtg acgccgcacc agtcatgatc aaatttaacg ggtaattggt cgtaaaaccg
    13081 acgagcaaaa gtaaacgcat tagagaaaaa gcggtttagc gcctcatcgt gtttgcttaa
    13141 taacggatac agcgccccct ggcgattgcc ggaagcaccc agcgcggggg cttcatccgc
    13201 gcaataaagc gctacctgcc agccgcgccg taataacgcc agcgacaaca acgcgctggc
    13261 aataccaccg ccgataatcg ctacttcccg tttgctgctg cccgtacgat taaaccacgg
    13321 cgtggagcag gggagtggta atgtctgttc catcacccca caaagcattt cccgtttgcg
    13381 cccaaagccc ttacgttttt gcatcgtgaa tccggcttcc tgcaaaccgc ggcggacaaa
    13441 accggcagac gtaaatgtcg ccagcgtgcc gcctggacgc gccaaccttg ccatggcgtt
    13501 aaacagattt tgcgtccaca tatccgggtt tttagctggc gcaaagccgt ccagaaacca
    13561 ggcatctact ttttgattta gcgaatcgtc cagttggctg gtcagttcgt taatatcgcc
    13621 aaaccataaa tccagcgtca cgcggccttc attgagcaat aaacgatggc aaccgggcaa
    13681 gggcattggc cactgcgcct gaagttgttc tgcccacgga gccagttccg gccagtgttg
    13741 atgcgctaag gctaaatccg cacgggtgag gggaaatttc tcaaaactaa tgaaatgtaa
    13801 gcgttgtaat tgcgcttgcg gatgcgcttc gcgaaactga tcaaatgcct gccatagcgt
    13861 caggaagttt aatccggtgc cgaagccgct ctctgctacc acaaacagag gatgtggatg
    13921 ctcaggaaag cgtacctcta attggttgcc tcccagaaaa acataacgcg tctcttccag
    13981 cccgttatcg ttggaaaaat agacatcgtc aaaatctcgg gaaacaggtg taccctcagc
    14041 attaaattcg aggttggcag gttgtatgga gtagtgtttc acgtaagtta ctcgtcttac
    14101 aggcggtggc tcgatcttag cgatgtgtgt aaggctgcgc aaatttctct attaaatggc
    14161 tgatcggact tgttcggcgt acaagtgtac gctattgtgc attcgaaact tactctatgt
    14221 gcgacttaca gaggtattga atgaaacgtg cagtgattac tggcctgggc attgtttcca
    14281 gcatcggtaa taaccagcag gaagtcctgg catctctgcg tgaaggacgt tcagggatca
    14341 ctttctctca ggagctgaag gattccggca tgcgtagcca cgtctggggc aacgtaaaac
    14401 tggataccac tggcctcatt gaccgcaaag ttgtgcgctt tatgagcgac gcatccattt
    14461 atgcattcct ttctatggag caggcaatcg cagatgcggg cctttctccg gaagcttacc
    14521 agaataaccc gcgcgttggc ctgattgcag gttctggcgg cggttccccg cgtttccagg
    14581 tattcggcgc tgacgcaatg cgcggcccgc gcggcctgaa agcggttggc ccgtatgtgg
    14641 tcaccaaagc gatggcatcc ggcgtttctg cctgcctcgc cacgccgttt aaaattcatg
    14701 gcgttaacta ctccatcagc tccgcgtgtg cgacttccgc acactgtatc ggtaacgcag
    14761 tagagcagat ccaactgggc aaacaggaca tcgtgtttgc tggcggcggc gaagagctgt
    14821 gctgggaaat ggcctgcgaa ttcgacgcga tgggtgcgct gtctactaaa tacaacgaca
    14881 ccccggaaaa agcctcccgt acttacgacg ctcaccgtga cggtttcgtt atcgctggcg
    14941 gcggcggtat ggtagtggtt gaagagctgg aacacgcgct ggcgcgtggt gctcacatct
    15001 atgctgaaat cgttggctac ggcgcaacct ctgatggtgc agacatggtt gctccgtctg
    15061 gcgaaggcgc agtacgttgt atgcagatgg cgatgcatgg cgttgatacc ccaatcgatt
    15121 acctgaactc tcacggtact tcgactccgg ttggcgacgt gaaagagctg gcagctatcc
    15181 gtgaagtgtt cggcgataag agcccggcga tttctgcaac caaagccatg accggtcact
    15241 cactgggcgc tgcgggtgtg caggaagcta tctactctct gctgatgctg gaacacggct
    15301 ttatcgcccc gagcatcaac attgaagagc tggacgagca ggctgcgggt ctgaacatcg
    15361 tgaccgaaac gaccgatcgc gaactgacca ctgttatgtc taacagcttc ggcttcggcg
    15421 gcaccaacgc cacgctggta atgcgcaagc tgaaagatta attcgcagta ggccggagta
    15481 ggtcggataa gacgcgccag cgtcgcatcc gacgttacgc gccaatgcgg cctccggcac
    15541 taacgcaaaa gggaacctga tggttccctt tttcacatca ttgacaatcg ccaccagttc
    15601 caggcaaact tcccgctttg tcgatttcct tctgaaaaga cgtacgcgtt aaatcctgcc
    15661 aacgcaccgt aaccctgaaa ccagagagat gagacgggga tactcctcgc cttgcgctgc
    15721 attctggagt aatgcatgac tgctgtaagc caaaccgaaa cacgatcttc tgccaatttt
    15781 tcgctcttcc gcatcgcttt tgcggttttt ctcacctaca tgaccgtagg gctgccgttg
    15841 ccggttatcc cgctgtttgt tcatcatgaa ctgggctatg gcaataccat ggtcggcatt
    15901 gccgtcggga ttcagtttct ggctacggtg ctgacgcgtg gctatgccgg gcgactggcc
    15961 gatcaatatg gcgcaaaacg ttcggcgctt caggggatgt tagcttgtgg tctggctggc
    16021 ggcgcgttgc tgctggcggc gattttgcct gtctccgcac cgttcaaatt tgccctgttg
    16081 gtcatcgggc gtttgattct tgggtttggt gaaagccagt tactgacagg cgctctgacc
    16141 tgggggttag gcatcgtagg gccaaaacac tctggcaaag tgatgtcatg gaatggaatg
    16201 gcgatttacg gtgccctcgc tgttggtgct ccgcttggcc tgttgattca tagccattac
    16261 ggttttgccg cactggcgat caccacaatg gtattaccct tactggcgtg ggcctgtaac
    16321 ggcacagtgc gcaaagtacc ggccctggcg ggagaacgtc catcgctgtg gagcgttgtc
    16381 gggtttatct ggaaaccagg gttagggctg gcactacaag gcgttggttt tgcggttatc
    16441 gggactttcg tttcgctcta ctttgccagc aaaggatggg cgatggcggg ctttactctt
    16501 accgagtttg gcggcgcatt tgtcgtgatg cgcgtcatgt ttggctggat gccggaccgt
    16561 tttggcggcg tgaaagtggc gattgtctct ctgcttgtag aaacggtggg cttgttgctg
    16621 ctctggcaag ccccaggtgc gtgggtcgca ttagcgggcg cggcgttaac cggagccgga
    16681 tgttcgctta tctttcctgc gctgggcgtg gaggtggtta aacgcgtccc ctcacaagtt
    16741 cgcggcaccg cactgggcgg ttacgccgcg tttcaggata tcgccctcgg cgtctccggg
    16801 ccgctggcgg gaatgctggc gaccacgttt ggttactctt cggtatttct tgccggggcg
    16861 atctctgcgg tgctgggaat tattgtcacg atactgtcat ttcgtcgggg ctaacgaacc
    16921 agccagacca gcatcaggat aatcgcgact aacagtatcc acagcgcggg acgcgttgcc
    16981 atattttgca acgtgtccat catcggtgga aaaggattaa gcagcggttg catatcacgc
    17041 ggatcaatcc ctttaacccg ccgttgataa agctgattaa gaatatcctc ggcctgggca
    17101 gaagaaagcg atgattgcgc agaaagacca tatttttgct ggatatacgc cgataacgct
    17161 gccagttcac tggcatctaa aggttgtttt agcgccatct gtagtgattc cagcgtcggc
    17221 gtattttgct ggcttaacgt ctggcgcgcc tgcaaccagg tcaccagatg gttaaacagt
    17281 ttcgctggaa ttaactcgcc atcgttcacc ccggaaagtt ccagcatcga ttgccagatc
    17341 tgtttgctgg gttcccccgt tgccgccgca agtttggtca ccagctgttt tagcgcattg
    17401 tgctccgccg gtaataaagg acggtcggtc gcctcgcgct gctgtggttg cggaataacc
    17461 atcttccctt cctgcaacag ggtgagaatg gtttttaatt gctccggtga gagctgattc
    17521 agcggcgtct gaccaaagtt atgacggata taatccgtga ccgcctgacg attattcccc
    17581 agacgtaaat actcccctaa ttgcgctaaa agctggcggg cagaatggct tttttgcgcg
    17641 gccagcagac gttgcgccag attatgctca gcggcaggga agtgacgcga aagcagcggt
    17701 gaatctcccg acagaccaat atcgtgcctg atacccgccc acagttctgc tctttgttgt
    17761 tgcgtcagtg aggtcacttt cgtcattaag ctttccagcg aagtacgttg ctgactggat
    17821 aaaggctgat tgcccgcgcc agacggcagg ttatctccct gacctggtgg ttgcccagga
    17881 ggagggccgg aaataggttg tatcattacg tatccttata cctgaaatct tcgcaagtat
    17941 gcctggccgc gagattatgg cacacttgtc cggttaactc tcgtctcata caggtaacac
    18001 aaacgtgaaa atccttgttg atgaaaatat gccttatgcc cgcgacttat ttagccgttt
    18061 gggtgaggtg accgcggttc ccgggcgtcc aatccccgtc gctcaactgg cagacgcgga
    18121 tgcgctgatg gtgcgttcgg tcacgaaagt gaatgaatct ttgctggcag gaaaacccat
    18181 taaatttgtt ggcactgcca ctgcggggac cgaccatgtc gatgaagcat ggttgaagca
    18241 ggcgggaatt ggtttttccg ctgcacctgg ctgtaatgcg attgcggtgg tggaatatgt
    18301 tttctcctca ctactgatgc ttgccgaacg cgatggattt tcactgcacg accgtacggt
    18361 ggggatcgtg ggcgttggta acgttggacg tcgattgcag gcgcgactgg aagcgttagg
    18421 gattaaaaca ttgctttgcg atccgcctcg cgctgaccgt ggggatgagg gtgatttccg
    18481 ctcgctggat gaattagttc agcatgcgga tattctgact ttccatacgc cactcttcaa
    18541 agatgggccg tacaaaacgc tgcatctggc ggatgaaaaa ctgatccgta gcctgaagcc
    18601 gggagcgatt ctgattaacg cctgccgtgg cgcagtcgtc gataatactg cgttgctgac
    18661 ctgcctgaat gaaggccaga agttaagcgt agtgctggat gtctgggaag gcgaaccgga
    18721 acttaacgtg gagttgctga caaaagtgga tatcggcacg ccgcatatcg caggttatac
    18781 cctggaaggt aaagcacgcg gtactacgca agtgtttgaa gcctatagca agtttattgg
    18841 gcatgaacag cacgttgcgc tggatacatt actgcccgcg ccagagtttg gtcgcattac
    18901 gctgcatggc ccgctcgatc aaccgacgct gaaaaggctg gtgcatttgg tgtatgatgt
    18961 gcgccgcgat gacgcaccgc tgcgtaaagt cgccgggata ccgggtgagt tcgataaact
    19021 gcgcaaaaac tatcttgagc gccgtgaatg gtcatctctg tatgtaattt gtgatgacgc
    19081 cagtgcggca tcattgctgt gtaaactggg ttttaacgcc gttcatcatc cggcacgtta
    19141 atctcttctt catgctctct gctgtaacat tggcagggag ctttgctatt tctggagtaa
    19201 accaccatgt ctgaaggctg gaacattgcc gtcctgggcg caactggcgc tgtgggcgaa
    19261 gccctgcttg aaacgctggc tgaacgtcag ttcccggttg gggaaattta tgcactggca
    19321 cgtaacgaaa gcgcaggcga acaactgcgc tttggtggta aaacaatcac tgtgcaggat
    19381 gccgctgaat tcgactggac gcaggcgcag ctggcatttt ttgtcgcagg caaagaagct
    19441 accgctgcct gggttgaaga agcgaccaac tcaggttgcc tggtgatcga cagcagcgga
    19501 ctgtttgctc tcgaacccga cgtaccgctg gtggtgccgg aagtaaaccc gtttgtactg
    19561 accgattacc gcaaccggaa tgtcatcgcc gtaccagaca gtctgaccag ccagctgctg
    19621 gcggcactga aaccgttaat cgatcagggc ggtttgtcgc gtatcagtgt taccagcctg
    19681 atttcagcct ccgcccaggg caaaaaagcg gtcgatgcgt tagcggggca gagtgcgaaa
    19741 ttgctcaacg gcattccgat tgacgaagaa gatttcttcg gtcgtcaact ggcgttcaat
    19801 atgctgccgt tgttgccgga tagcgaaggt agcgtgcgtg aagaacgtcg tatcgttgac
    19861 gaagtacgca aaatcctgca ggacgaaggg ctgatgattt cggcaagcgt cgtccaggca
    19921 ccggtattct acggtcatgc ccagatggtc aactttgaag cactgcgtcc gctggcggca
    19981 gaagaagcgc gtgatgcgtt tgctcagggc gaagatattg tgctctctga agagaacgaa
    20041 ttcccgactc aggtagggga tgcttcgggt acgccgcatc tttctgttgg ctgcgtgcgt
    20101 aatgactacg gtatgccgga gcaagtccag ttctggtcgg tggccgataa cgttcgcttt
    20161 ggcggcgcgc tgatggcagt aaaaatcgcc gagaaactgg tgcaggagta tctgtactaa
    20221 tgtccgacca gcaacaactg ccagtttata aaattgcgct gggcattgag tacgacggca
    20281 gtaagtatta cggctggcaa cggcagaatg aagtccgcag tgtgcaggag aagctggaaa
    20341 aggcgctctc ccaggtggcg aacgaaccca tcaccgtctt ctgcgccggg cgtactgacg
    20401 caggggtaca cggtaccggg caggtggtgc atttcgaaac aaccgcgcag cgcaaagacg
    20461 cggcgtggac cttgggcgta aatgcgaatt tacctggtga catcgccgtg cgttgggtta
    20521 aagctgtacc tgatgatttt catgcccgat ttagcgccac ggctcgccgt tatcgctaca
    20581 tcatctacaa tcatcggctg cgcccggcgg tattgagtaa aggggtaacc catttttacg
    20641 aaccgctgga cgctgaacgg atgcatcggg ctgcgcaatg cttgctgggc gagaatgatt
    20701 tcacctcgtt ccgtgcggtg cagtgccagt cccgaacccc gtggcgcaac gttatgcata
    20761 ttaatgtcac gcgtcacggt ccttatgtgg tggtagatat caaagcgaat gcctttgtac
    20821 atcatatggt caggaatatt gtcggcagcc tgatggaagt cggtgcccac aaccagccgg
    20881 agagatggat agcagagttg ctggcggcaa aggacagaac gctggcggca gcaacggcaa
    20941 aagcggaagg gctgtatctg gtcgcggtgg attaccctga ccggtatgat cttccaaaac
    21001 cgccaatggg cccgctattt ctggcggact aacgagaata atgctcggaa catttcaggc
    21061 tgtagccaac gtagttacag cctgaaagat gacgagtaca aggcataggc aaatatggac
    21121 ctgatttatt tcctcatcga ttttatcctg cacattgacg tgcatctggc agagctggtc
    21181 gcggagtacg gcgtctgggt ttatgccatt ctgtttttga ttctgttctg cgaaacgggg
    21241 ctggtggtaa cgccattcct gcctggtgat tcgctgctgt ttgttgctgg tgcattggcg
    21301 tcgctggaaa ctaacgatct caatgtccat atgatggtgg tgcttatgtt gattgccgct
    21361 attgtcggtg atgcagtgaa ctataccatt ggacggttat ttggtgacaa actgttcagt
    21421 aacccggatt cgaaaatctt tcgtcgcagt tatcttgata agactcatca gttttacgag
    21481 agacatggcg gcaaaacgat tattctcgcc cgttttgtac cgatcgtcag aacgttcgcg
    21541 ccgtttgttg cgggaatggg ccacatgtcg taccgtcatt tcgccgctta taacgtgatc
    21601 ggcgcactgt tgtgggtact gatttttacc tacgcgggtt atttcttcgg cacgctcccc
    21661 ttcattcaga gcaaccttaa gctgatgatt gttggcatta tttttgtttc aatattgcct
    21721 ggggttttcg aatttattcg tcacaaacgc gctgcggcac gcgccgcaaa ataaaaagta
    21781 actccgcggt tcgaccactt ttttatccaa agtttcgggc tgttatgttt taatgtgcaa
    21841 cattcatggt ctgttggggg caaaaatggc attatgcgtc cccaaagata aaactggcat
    21901 cgaaccaggt tcagacagaa aggtccctaa tgagctggat tgaacgaatt aaaagcaaca
    21961 ttactcccac ccgcaaggcg agcattcctg aaggggtgtg gactaagtgt gatagctgcg
    22021 gtcaggtttt ataccgcgct gagctggaac gtaatcttga ggtctgtccg aagtgtgacc
    22081 atcacatgcg tatgacagcg cgtaatcgcc tgcatagcct gttagatgaa ggaagccttg
    22141 tggagctggg tagcgagctt gagccgaaag atgtgctgaa gtttcgtgac tccaagaagt
    22201 ataaagaccg tctggcttct gcgcagaaag aaaccggcga aaaagatgcg ctggtggtga
    22261 tgaaaggcac tctgtatgga atgccggttg tcgctgcggc attcgagttc gcctttatgg
    22321 gcggttcaat ggggtctgtt gtgggtgcac gtttcgtgcg tgccgttgag caggcgctgg
    22381 aagataactg cccgctgatc tgcttctccg cctctggtgg cgcacgtatg caggaagcac
    22441 tgatgtcgct gatgcagatg gcgaaaacct ctgcggcact ggcaaaaatg caggagcgcg
    22501 gcttgccgta catctccgtg ctgaccgacc cgacgatggg cggtgtttct gcaagtttcg
    22561 ccatgctggg cgatctcaac atcgctgaac cgaaagcgtt aattggcttt gccggtccgc
    22621 gtgttatcga acagaccgtt cgcgaaaaac tgccgccagg attccagcgc agtgaattcc
    22681 tgatcgagaa aggcgccatc gacatgatcg tccatcgtcc ggaaatgcgc ctgaaactgg
    22741 cgagcattct ggcgaagttg atgaatctgc cagcgccgaa tcctgaagcg ccgcgtgaag
    22801 gcgtagtggt acccccggta ccggatcagg aacctgaggc ctgataactg ataagggcag
    22861 ggccactggc tctgcccttt tgctattctc accgtaacga atcagcggat accatgatta
    22921 tcaaacgcac tcctcaagcc gcgtcgcctc tggcttcgtg gctttcttat ctggaaaacc
    22981 tgcacagtaa aactatcgat ctcggccttg agcgcgtgag ccaggtcgcg gcgcgtcttg
    23041 gcgtcctgaa accagcgcca tttgtgttta ccgttgcggg tacgaatggc aaaggcacca
    23101 cctgccgtac gctggagtcg attctgatgg cggcagggta caaagtgggc gtctacagtt
    23161 caccgcatct ggtgcgttat accgagcgcg tacgtgtgca ggggcaggaa ttgccggaat
    23221 cggcccacac cgcctctttt gcggagattg aatcggcacg cggtgatatt tccctgacct
    23281 atttcgagta cggtacgctg tcggcgttgt ggctgttcaa acaggcacaa cttgacgtag
    23341 tgattctgga agtagggctg ggcggtcgtc tggacgcaac caatattgtc gatgccgatg
    23401 tggctgtagt aaccagcatt gcgctggatc ataccgactg gctgggacca gatcgcgaaa
    23461 gtattggtcg cgagaaagca ggcatctttc gcagcgcaaa accggcaatt gtcggtgagc
    23521 cggaaatgcc ttctaccatt gctgatgtgg cgcaggaaaa aggtgcactg ttacaacgtc
    23581 ggggcgttga gtggaactat tccgtcaccg atcatgactg ggcgtttagc gatgctcacg
    23641 gcacgctgga aaatctgccg ttgccgcttg tcccgcaacc gaatgccgca acagcgctgg
    23701 cggcactgcg tgccagcggg ctggaagtca gtgaaaatgc cattcgcgac gggattgcca
    23761 gcgcaatttt gccgggacgt ttccagattg tgagcgagtc gccacgcgtt atttttgatg
    23821 tcgcgcataa tccacatgcg gcggaatatc tcaccgggcg tatgaaagcg ctaccgaaaa
    23881 acgggcgcgt gctggcggtt atcggtatgc tacatgataa agatattgcc ggaactctgg
    23941 cctggttgaa aagcgtggtt gatgactggt attgtgcgcc actggaaggg ccgcgcggtg
    24001 ccacggcaga acaactgctt gagcatttgg gtaacggcaa atcatttgat agcgttgcgc
    24061 aggcatggga tgccgcaatg gcggacgcta aagcggaaga caccgtgctg gtgtgtggtt
    24121 ctttccacac ggtcgcacat gtcatggaag tgattgacgc gaggagaagc ggtggcaagt
    24181 aagtttcaga atcggttagt gggcacgatc gtgctggtgg cgctgggggt gattgtactt
    24241 ccagggctgc tggacgggca gaaaaaacat tatcaggatg agtttgcggc tatcccgctg
    24301 gttccaaaag cgggcgatcg cgatgagcct gatatgatgc cagccgccac ccaggcgttg
    24361 ccgacgcagc cgccggaagg cgcagcggaa gaggtgcggg caggtgatgc cgcagcaccg
    24421 tcgctcgatc cggccactat tgcagccaat aacaccgagt ttgaaccgga acctgcaccg
    24481 gtcgccccac cgaagccgaa accggtggag ccgcctaaac cgaaggttga agcaccacct
    24541 gcgccgaagc cagaaccgaa gccggtcgtg gaagaaaaag cagcgccaac gggtaaagcc
    24601 tatgttgtgc aactgggtgc gctgaaaaat gccgataaag tgaatgagat tgtcggtaag
    24661 ctgcgcggtg ccggttatcg ggtttatacg tcgccatcca cgccagtgca gggtaaaatt
    24721 acccgtattc tggttgggcc ggatgcctcg aaagataagc tgaaaggttc gctgggtgag
    24781 ttgaagcaac tttctggctt aagtggcgtg gtaatgggct atacgccgaa ttaatacagt
    24841 cttgcctgat gcgacgctgg cgcgtcttat caggcctggg cagtgttaga accgtaggtc
    24901 ggataaggcg tttacgccgc atccgacacg cattgcacga tgccgcaaag gcataaaaag
    24961 tcgatggcgt tgaatatttt ttcagcgcca tttttattga tgcgtgggaa ggaaatccct
    25021 acgcaaacgt tttctttttc tgttagaatg cgccccgaac aggatgacag ggcgtaaaat
    25081 cgtgggacac atatggtctg gattgattac gccataatcg cggtgattgc tttttcctct
    25141 ctggttagcc tgatccgcgg ctttgttcgt gaagcgttat cgctggtgac atggggttgt
    25201 gctttctttg ttgccagtca ttactacact tacctgtcag tctggtttac gggctttgaa
    25261 gacgaactgg ttcgaaatgg aattgccatc gcggtactgt ttatcgctac cctgattgtt
    25321 ggtgctatcg tgaacttcgt gataggccag ttggtggaga aaacggggtt gtcaggcacc
    25381 gatcgggtgc tgggcgtctg tttcggtgcg ttgcgcggtg tgttgattgt tgccgccatt
    25441 ctcttctttc tcgactcctt taccggggtg tcgaaaagcg aagactggag caaatcacag
    25501 ctgatcccgc agttcagttt tatcatcaga tggttttttg attatctgca aagctcgtca
    25561 agtttcttgc ccagagcgta agtgctctga gatgtggctt aacgaggaaa aagacgtatg
    25621 tgcggtattg tcggtatcgc cggtgttatg ccggttaacc agtcgattta tgatgcctta
    25681 acggtgcttc agcatcgcgg tcaggatgcc gccggcatca tcaccataga tgccaataac
    25741 tgcttccgtt tgcgtaaagc gaacgggctg gtgagcgatg tatttgaagc tcgccatatg
    25801 cagcgtttgc agggcaatat gggcattggt catgtgcgtt accctacggc tggcagctcc
    25861 agcgcctctg aagcgcagcc gttttacgtt aactccccgt atggcattac gcttgcccac
    25921 aacggcaatc tgaccaacgc tcacgagttg cgtaaaaaac tgtttgaaga aaaacgccgc
    25981 cacatcaaca ccacttccga ctcggaaatt ctgcttaata tcttcgctag cgagctggac
    26041 aacttccgcc actacccgct ggaagccgac aatattttcg ctgccattgc tgccacaaac
    26101 cgcttaatcc gcggcgcata tgcctgtgtg gcgatgatta tcggccacgg tatggttgct
    26161 ttccgcgatc caaacgggat tcgtccgctg gtactgggaa aacgtgatat tgacgagaac
    26221 cgtacagaat atatggtcgc ttccgaaagc gtagcgctcg atacgctggg ctttgatttc
    26281 ctgcgtgacg tcgcgccggg cgaagcgatt tacatcactg aagaagggca gttgtttacc
    26341 cgtcaatgtg ctgacaatcc ggtcagcaat ccgtgcctgt ttgagtatgt atactttgct
    26401 cgcccggact cgttcatcga caaaatttcc gtttacagcg cgcgtgtgaa tatgggcacg
    26461 aaactgggcg agaaaattgc ccgcgaatgg gaagatctgg atatcgacgt ggtgatcccg
    26521 atcccagaaa cctcgtgtga tatcgcgctg gaaattgctc gtattctggg caaaccgtac
    26581 cgccagggct tcgttaaaaa ccgctatgtt ggccgcacct ttatcatgcc gggccagcag
    26641 ctgcgtcgta agtccgtgcg ccgtaaactg aatgccaacc gcgccgagtt ccgcgataaa
    26701 aacgtcctgc tggtcgacga ctccatcgtc cgtggcacca cttctgagca gattatcgag
    26761 atggcacgcg aagccggagc gaagaaagtg tacctcgctt ctgcggcacc ggaaattcgc
    26821 ttcccgaacg tttatggtat tgatatgccg agcgccacgg aactgatcgc tcacggtcgc
    26881 gaagttgatg aaattcgcca gatcatcggt gctgacgggt tgattttcca ggatctgaac
    26941 gatctgatcg acgccgttcg cgctgaaaat ccggatatcc agcagtttga atgctcggtg
    27001 ttcaacggcg tctacgtcac caaagatgtt gatcagggct acctcgattt cctcgatacg
    27061 ttacgtaatg atgacgccaa agcagtgcaa cgtcagaacg aagtggaaaa tctcgaaatg
    27121 cataacgaag gatgatgcct tcgctgaggg tgccggtctg gcaccctgac ttgcaactcc
    27181 cgccgaaata ctgcaaagtc tgcctgcaag tctgacaggg caactattta tgaaacgact
    27241 cattgtaggc atcagcggtg ccagcggcgc gatttatggc gtgcgcttat tacaggttct
    27301 gcgcgatgtc acagatatcg aaacgcatct ggtgatgagc caggcggcgc gccagacctt
    27361 atccctcgaa acggattttt ccctgcgcga agtgcaggca ttagctgatg tcacgcacga
    27421 tgcgcgcgat atttccgcca gcatctcttc cggttctttc cagacgctgg ggatggtgat
    27481 tttaccctgt tcaatcaaaa ccctttccgg cattgtccat agctatactg atggcttact
    27541 gacccgtgcg gcagatgtgg tgctgaaaga gcgtcgcccg ttggtgctct gcgtgcgtga
    27601 aacaccattg cacttaggcc atctgcgttt aatgactcag gcggcagaaa tcggtgcggt
    27661 gattatgcct cccgttccgg cgttttatca tcgcccgcaa tcccttgatg atgtgataaa
    27721 tcagacggtt aatcgtgttc ttgaccagtt tgcaataacc cttcctgaag atctctttgc
    27781 ccgctggcag ggcgcataac tccgctgttg ccctgtttca gggcaatttt gcaaccgcga
    27841 tcaaatcctc gacattttgt ttctgccatt caatcgaaac gctgcgattc aaccgctata
    27901 cctgctatct tcaacttcag gacaataatg caacgtctta ttaacatatt taacgttgaa
    27961 tgttactgtt gtcgtcaaga tggcataaga cctgcatgaa agagcctgca aacacacaac
    28021 acaatacaca acataaaaaa gccattttca cttgagggtt atgtatgaag aagtcgattc
    28081 tcgctctgtc tttgttagtc ggtctctccg cagcggcttc cagctatgcg gcgctaccgg
    28141 agacggtacg tatcggaacc gataccacct atgcgccgtt ctcgtcgaaa gatgctaaag
    28201 gtgattttgt tggctttgat atcgatctcg gtaacgagat gtgcaaacgg atgcaggtga
    28261 aatgtacctg ggttgccagt gactttgacg cgctgatccc ctcactgaaa gcgaaaaaaa
    28321 tcgacgccat tatttcgtcg ctttccatta ccgataaacg tcagcaggag attgccttct
    28381 ccgacaagct gtacgccgca gattctcgtt tgattgctgc caaaggttca ccgattcagc
    28441 caacgctgga ttcactgaaa ggtaaacatg ttggtgtgct gcagggatca acccaggaag
    28501 cttacgctaa cgagacctgg cgtagcaaag gcgtggatgt ggtggcctat gccaaccagg
    28561 atttggtcta ttccgatctg gctgcaggac gtctggatgc tgcgttacaa gatgaagttg
    28621 ctgccagcga aggattcctc aagcaacctg ctggtaaagc tttcgccttt gctggctcat
    28681 cagtaaaaga caaaaaatac ttcggtgatg gcaccggtgt agggctacgt aaagatgatg
    28741 ctgaactgac ggctgccttc aataaggcgc ttggcgagct gcgtcaggac ggcacctacg
    28801 acaagatggc gaaaaagtat ttcgacttta atgtctacgg tgactgatac gtcgctggga
    28861 agctgtacct gatggaatga tcatcatggt gcacgccagg tttgttgcac tatcgtggtg
    28921 cattgaaatg catacttaag catttttaat gaaaaataat acgtctaacg gggcggaata
    28981 ttttgccttg atggtcaatt ttatggcacg ataagtgtaa caaacctgta aatattccct
    29041 ataaaaagac tgtcagttga ggacattatg aaaaaactgg tgctatcgct ctctctggtt
    29101 ctggccttct ccagcgcaac tgcggcattt gctgcgattc cgcaaaacat ccgcatcggt
    29161 accgacccga cctatgcgcc atttgaatca aaaaattcac aaggcgaact ggttggcttc
    29221 gatatcgatc tggcaaagga attatgcaaa cgcatcaata cgcaatgtac gtttgtcgaa
    29281 aatccgctgg atgcgttaat cccgtcctta aaagcgaaga agattgacgc catcatgtca
    29341 tcgctttcca ttacggaaaa acgtcagcaa gaaatagcct tcaccgacaa actgtacgct
    29401 gccgattctc gtttggtggt ggcgaaaaat tctgacattc agccgacagt cgagtcgctg
    29461 aaaggcaaac gggtaggcgt attgcagggc accacccagg agacgttcgg taatgaacat
    29521 tgggcaccaa aaggcattga aatcgtctcg tatcaggggc aggacaacat ttattctgac
    29581 ctgactgccg gacgtattga tgccgcgttc caggatgagg tcgctgccag cgaaggtttc
    29641 ctcaaacaac ctgtcggtaa agattacaaa ttcggtggcc cgtctgttaa agatgaaaaa
    29701 ctgtttggcg tagggaccgg catgggcctg cgtaaagaag ataacgaact gcgcgaagca
    29761 ctgaacaaag cctttgccga aatgcgcgct gacggtactt acgagaaatt agcgaaaaag
    29821 tacttcgatt ttgatgttta tggtggctaa tcgccatcgg tgaaagaaag cccgctccct
    29881 tcgggtaacc ggagggagac aagaggcggt aattcaccac acacgacagg acaggcagca
    29941 tgttgtatgg gttttcaggt gttattttac agggtgcgct cgtcacgctg gagctggcta
    30001 tcagctctgt agtgctcgct gtaatcatcg gtttaattgg cgctggcggt aagctctcgc
    30061 aaaatcggct ttcggggctg attttcgaag ggtacaccac gctgattcgt ggcgtgccgg
    30121 acttagtgtt gatgctgctg attttctacg gtttgcagat tgcgctaaac acggtgacgg
    30181 aggcgatggg cgtcgggcag attgatatcg atccgatggt cgctggtatt atcactctcg
    30241 gttttatcta cggtgcttat tttaccgaaa cgttccgtgg tgcttttatg gcagtgccga
    30301 aaggacatat agaggcggcg acggcgttcg gttttactcg tgggcaagtg tttcggcgga
    30361 tcatgtttcc ggcgatgatg cgttacgccc tgccaggcat tggcaacaac tggcaggtga
    30421 tcctcaaatc taccgcactg gtttcgttac tcggcctgga agatgtggtc aaagccaccc
    30481 aactggcagg caaaagtacc tgggaaccgt tctatttcgc catcgtctgt ggcgtgattt
    30541 acctggtttt caccactgtt tccaatggtg tgctgctgtt ccttgagcgc cgctactccg
    30601 tgggtgtgaa gagggctgac ctgtgatcga aatcttacat gaatactgga aaccgctgct
    30661 gtggaccgac ggttatcgct ttactggtgt ggcgatcact ctgtggctgc ttattttgtc
    30721 ggtagtgata ggcggagtcc tggcgctgtt tctggcgatt ggtcgtgtct ccagtaataa
    30781 atacatccag tttccaatct ggttatttac ctatattttt cgcggtacgc cgctgtatgt
    30841 tcagttgctg gtgttctatt ccggcatgta cacgcttgag attgttaagg gaaccgaatt
    30901 ccttaacgct ttcttccgca gtggcctgaa ctgtaccgtg ctggcgctga cgcttaacac
    30961 ctgcgcttac actaccgaga tttttgctgg ggcaatccgt tcggttccgc atggggaaat
    31021 tgaagccgcc agagcctatg gcttctcgac ttttaaaatg tatcgttgca ttattttgcc
    31081 ttctgcgctg cgtattgcgt taccggcata cagcaacgaa gtgatcctga tgctgcactc
    31141 tactgcgctg gcgtttacgg ccactgtgcc ggatctgctg aaaatagccc gcgatattaa
    31201 cgccgccacg tatcaacctt ttaccgcctt cggcattgcc gcggtgctct atttaatcat
    31261 ctcttatgtc ctgatcagcc tctttcgcag agcggaaaaa cgctggttgc agcatgtgaa
    31321 accttcttca acgcactgag aatacgatgt ccgagaataa attaaacgtt atcgatttgc
    31381 acaaacgcta cggcgaacat gaagtgctga aaggggtctc actgcaagcg aatgccggag
    31441 atgtaataag catcatcgga tcgtcgggat cggggaaaag tacctttctg cgctgcatta
    31501 acttcctcga aaaaccgagt gaagggtcga tcgtggtcaa tggccagacg atcaatctgg
    31561 tgcgcgacaa agacggtcaa ctcaaagtcg ccgataaaaa tcaactgcgc ttactgcgca
    31621 cacgcctgac gatggtattc cagcacttca atctctggag ccatatgacg gtgctggaaa
    31681 acgtcatgga agcgccgatt caggtgttgg gcctgagcaa gcaggaagcg cgcgagcggg
    31741 cggtgaagta tctggcgaaa gtcgggatag acgaacgtgc gcaggggaaa tatccggtgc
    31801 atctttccgg cggtcagcaa cagcgtgttt ctatcgcgcg ggcgctggcg atggaaccgg
    31861 aagttttact gtttgatgaa cctacctcgg cgctcgatcc tgaactggta ggcgaagtgt
    31921 tacgtattat gcagcaactg gcagaagagg ggaaaaccat ggtggtagtg actcacgaaa
    31981 tgggctttgc tcgccatgtt tctactcacg tcattttcct ccatcagggg aaaatagaag
    32041 aagagggcgc gccggagcag ttatttggca acccgcaaag ccctcgtctg caacagttcc
    32101 ttaagggatc gctgaaataa tttcctctcc ggtggctaac gctgccggag tattttcatg
    32161 gggaatttgc gagtcagctt ccgctgaaat gtaaaagttg cagtccgcta aaaatacttc
    32221 tgaattcgct gtccaaagat aaaaactgcc tgctgcattg ggtgttaatc gtggttattg
    32281 tcgcgtcatc ttaaatttga ggggtgacgg aacgccatga caatatcgac aacttccacg
    32341 ccgcatgatg cggtatttaa atctttttta cgccatccag
//
LOCUS       DAZOZD010000022        31844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOZD010000022 DAZOZD010000000
VERSION     DAZOZD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.022"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(146..1504)
                     /gene="plaP"
                     /locus_tag="ACHUPB_000826"
     CDS             complement(146..1504)
                     /gene="plaP"
                     /locus_tag="ACHUPB_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0757719.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1494..1556)
                     /gene="yoeI"
                     /locus_tag="ACHUPB_000827"
     CDS             complement(1494..1556)
                     /gene="yoeI"
                     /locus_tag="ACHUPB_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0757720.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1771..2700)
                     /locus_tag="ACHUPB_000828"
     CDS             complement(1771..2700)
                     /locus_tag="ACHUPB_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0757721.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2746..3570)
                     /locus_tag="ACHUPB_000829"
     CDS             complement(2746..3570)
                     /locus_tag="ACHUPB_000829"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0757722.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3653..3907)
                     /gene="yoeB"
                     /locus_tag="ACHUPB_000830"
     CDS             complement(3653..3907)
                     /gene="yoeB"
                     /locus_tag="ACHUPB_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0757723.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3904..4155)
                     /gene="yefM"
                     /locus_tag="ACHUPB_000831"
     CDS             complement(3904..4155)
                     /gene="yefM"
                     /locus_tag="ACHUPB_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0757724.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4438..4488
                     /gene="hisL"
                     /locus_tag="ACHUPB_000832"
     CDS             4438..4488
                     /gene="hisL"
                     /locus_tag="ACHUPB_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR0757725.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4464..4587
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4634..5533
                     /gene="hisG"
                     /locus_tag="ACHUPB_000833"
     CDS             4634..5533
                     /gene="hisG"
                     /locus_tag="ACHUPB_000833"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0757726.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5539..6843
                     /gene="hisD"
                     /locus_tag="ACHUPB_000834"
     CDS             5539..6843
                     /gene="hisD"
                     /locus_tag="ACHUPB_000834"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0757727.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6840..7910
                     /gene="hisC"
                     /locus_tag="ACHUPB_000835"
     CDS             6840..7910
                     /gene="hisC"
                     /locus_tag="ACHUPB_000835"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0757728.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7910..8977
                     /gene="hisB"
                     /locus_tag="ACHUPB_000836"
     CDS             7910..8977
                     /gene="hisB"
                     /locus_tag="ACHUPB_000836"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0757729.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8977..9567
                     /gene="hisH"
                     /locus_tag="ACHUPB_000837"
     CDS             8977..9567
                     /gene="hisH"
                     /locus_tag="ACHUPB_000837"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0757730.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9567..10304
                     /gene="hisA"
                     /locus_tag="ACHUPB_000838"
     CDS             9567..10304
                     /gene="hisA"
                     /locus_tag="ACHUPB_000838"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0757731.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10286..11062
                     /gene="hisF"
                     /locus_tag="ACHUPB_000839"
     CDS             10286..11062
                     /gene="hisF"
                     /locus_tag="ACHUPB_000839"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0757732.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11056..11667
                     /gene="hisIE"
                     /locus_tag="ACHUPB_000840"
     CDS             11056..11667
                     /gene="hisIE"
                     /locus_tag="ACHUPB_000840"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0757733.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11764..12741)
                     /gene="wzzB"
                     /locus_tag="ACHUPB_000841"
     CDS             complement(11764..12741)
                     /gene="wzzB"
                     /locus_tag="ACHUPB_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0757734.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12887..14053)
                     /gene="ugd"
                     /locus_tag="ACHUPB_000842"
     CDS             complement(12887..14053)
                     /gene="ugd"
                     /locus_tag="ACHUPB_000842"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR0757735.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14303..15709)
                     /gene="gndA"
                     /locus_tag="ACHUPB_000843"
     CDS             complement(14303..15709)
                     /gene="gndA"
                     /locus_tag="ACHUPB_000843"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0757736.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15873..17243)
                     /gene="cpsG"
                     /locus_tag="ACHUPB_000844"
                     /gene_synonym="manB"
     CDS             complement(15873..17243)
                     /gene="cpsG"
                     /locus_tag="ACHUPB_000844"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0757737.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(17348..18784)
                     /gene="cpsB"
                     /locus_tag="ACHUPB_000845"
     CDS             complement(17348..18784)
                     /gene="cpsB"
                     /locus_tag="ACHUPB_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0757738.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(18787..20010)
                     /gene="wcaI"
                     /locus_tag="ACHUPB_000846"
     CDS             complement(18787..20010)
                     /gene="wcaI"
                     /locus_tag="ACHUPB_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0757739.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(20007..20486)
                     /gene="gmm"
                     /locus_tag="ACHUPB_000847"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(20007..20486)
                     /gene="gmm"
                     /locus_tag="ACHUPB_000847"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0757740.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(20489..21454)
                     /gene="fcl"
                     /locus_tag="ACHUPB_000848"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(20489..21454)
                     /gene="fcl"
                     /locus_tag="ACHUPB_000848"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0757741.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(21457..22578)
                     /gene="gmd"
                     /locus_tag="ACHUPB_000849"
     CDS             complement(21457..22578)
                     /gene="gmd"
                     /locus_tag="ACHUPB_000849"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0757742.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(22604..23152)
                     /gene="wcaF"
                     /locus_tag="ACHUPB_000850"
     CDS             complement(22604..23152)
                     /gene="wcaF"
                     /locus_tag="ACHUPB_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0757743.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(23168..23914)
                     /gene="wcaE"
                     /locus_tag="ACHUPB_000851"
     CDS             complement(23168..23914)
                     /gene="wcaE"
                     /locus_tag="ACHUPB_000851"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0757744.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(23925..25142)
                     /gene="wcaD"
                     /locus_tag="ACHUPB_000852"
     CDS             complement(23925..25142)
                     /gene="wcaD"
                     /locus_tag="ACHUPB_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR0757745.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTKALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(25117..26334)
                     /gene="wcaC"
                     /locus_tag="ACHUPB_000853"
     CDS             complement(25117..26334)
                     /gene="wcaC"
                     /locus_tag="ACHUPB_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0757746.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(26331..26819)
                     /gene="wcaB"
                     /locus_tag="ACHUPB_000854"
     CDS             complement(26331..26819)
                     /gene="wcaB"
                     /locus_tag="ACHUPB_000854"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0757747.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(26822..27661)
                     /gene="wcaA"
                     /locus_tag="ACHUPB_000855"
     CDS             complement(26822..27661)
                     /gene="wcaA"
                     /locus_tag="ACHUPB_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR0757748.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(27754..29916)
                     /gene="wzc"
                     /locus_tag="ACHUPB_000856"
     CDS             complement(27754..29916)
                     /gene="wzc"
                     /locus_tag="ACHUPB_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0757749.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(29919..30362)
                     /gene="wzb"
                     /locus_tag="ACHUPB_000857"
     CDS             complement(29919..30362)
                     /gene="wzb"
                     /locus_tag="ACHUPB_000857"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0757750.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(30368..31507)
                     /gene="wza"
                     /locus_tag="ACHUPB_000858"
     CDS             complement(30368..31507)
                     /gene="wza"
                     /locus_tag="ACHUPB_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0757751.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         7706 a         8423 c         7978 g         7737 t
ORIGIN      
        1 attattcatg actggtggaa tgcagaccaa taaccaaatt ctctaaatta gaacaaatgg
       61 ttattaatga ggatttcgat aaaaaaaaat aaaaaaaccg gagccaaata ttcattcgac
      121 tccggttgat tattagaaaa gatgattatg caacgtcttc gtactgcgga actgggttac
      181 ggaagctctt ggtgacgcaa gccaggtaaa tcagaccgat agcagcccaa atcagaccca
      241 gaaccattga gctttcttcc aggttaaccc acagcgcacc aacggtcagc gcaccacaca
      301 tcggcaggaa cagatactgg aagtgatctt tcagcgtctt attacgcttc tcacggatcc
      361 agaactgcga gatgaccgac aggttaacga aggtgaacgc caccaacgca ccaaagttaa
      421 tcagcgctgt agccattacc aggtcgaagt tgattgccag cagggcaatc gcgccaacca
      481 ggatgatgtt catcgccgga gtacgccatt tcgggtgtac ataaccgaag aagcttttcg
      541 ggaatacgcc gtcacgaccc attacgtaca tcagacgcgc tacgcctgca tgcgccgcca
      601 taccggacgc cagtacggta atggtggaga agatcagcgc gccaacctgg aacgctttac
      661 ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg atctttaaag cgagagatat
      721 ccgggaagta cagctgcagg aagtaagttg caaagatgaa gatcatgccg ccaatcagcg
      781 cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc tttggtttct tccgacaggt
      841 tgctgatgcc gtcaaagccg gtaaaggaaa agcacaggat tgtcgccccg gtaatcatcg
      901 gaataacatg cgcatcgcca gaccagaacg gacgagtgct cgccagcgta ccagcgcctt
      961 caccttcaaa tacgccataa acaaccatgc ccagaatcac cgcgatcagc actacctgca
     1021 acacgacgat tacagtgttg aagttcgcta cggatttcag actacgcagg ttaaaggcgg
     1081 tcataaaggc caccagcgcc accacaaaca tccacgatgg gatggaaggc accagagctt
     1141 caaaatagat tttcgccagc agaatgttga tcatcggcgc gaacagatag tcgagcagag
     1201 aagaccaacc caccataaag ccgacagtcg ggctaatgga tttctgggcg taagtatatg
     1261 cagagccagc agaaggatag cggcgaacca gcttcccgta gctcagagcc gtaaacagga
     1321 tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc atccgtgagg cctgaaacga
     1381 taccaaaagt atcaaacagc gtcatcggct gcatataggc aagacccatc attacaaccg
     1441 gaactaacgt aagcgtttta cgcaattcca cgcgagaggt gtttggagta acgttatgcg
     1501 acatggtcat tctcctttac ggtgataacc gtcgcgtaag caaaaaattg ccccattttt
     1561 ttggattcct cagcgacaac aactgtcgat ttttagtaaa tatctatccg gtacgaagcc
     1621 cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata accgacgctg atgaaacgtc
     1681 ggtttttagt cattttttga cagcggcgca ttgtgcctaa atggggggga aatgacaaga
     1741 gaatgagaag cttgtcataa taatttttct ttaaatggct gattttccgt catcagattt
     1801 tgcataaaca ccggcgatcg cactatttgc taaaatttca tcccgccacc agccgctggc
     1861 aagccctgcg gtctgttcgt tccagccaat ccagacgggt tcaaagagag tttgggcgac
     1921 cactttcttc tcgattaacg cgccactatc gagaaaacgt tgcaccagat aacggggtaa
     1981 atagccgcag ccgaggccgc taatttgcag ctccagcttg gttttaaaat cgaaaacggt
     2041 aatggcttct tgttcgtcga gaagttgtga ggccgtagaa gcgcctgcct gcgcagtgtc
     2101 ccccaccaca atcgcccgat atcgcttaat gatacgacgg tttaacggct cttcttcctg
     2161 ggctaacgga tgatgaggcg cgaccgcaaa gacttgttcc agatcgccca accgcgagaa
     2221 accaaactca ctggaagatg gtggctcatg catcgcgcca acaataatat ccgctcgccc
     2281 ctgagtcagg gcatcccagg aaccaccgag tacgccattg ataaacttca ggcgcgtcac
     2341 gctatgatgt tgataaaaag cttcaatcag tggcgcaagc agagaaaaag gaaacgtgtc
     2401 gtccacgcca atcaccagct cgttttccca gccttcatgc agtttgatcg cctgcttttc
     2461 cagttctcgc acggtatgca gaacttcccg ccccttctct aataacattt ttccggtacg
     2521 ggtgaattta gcgcggtgac cgctacgatc aagtaattga atattaaggt cgctttccag
     2581 cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag agtttagccg acgccgccgc
     2641 aaaactgcct tctttttcta aggcatcgag gatcatcaac acatccagca gttgtttcat
     2701 acacgccccc ttgcagtgca tgcggcgtcc tgctggcgtg atcgtttact ccagcggcat
     2761 taccagcgga tcgggatact ggtattcaaa tcccagttca ttacaaatcc gactgccatc
     2821 aataatctta cctttgccgc tgtccagact atttctgaac tgcggaggtt ccagccccag
     2881 taaacgggcc atctgcggat agaaaacatt acgcgcaggg tgagctggcg cacatatatt
     2941 atagatgtgt ccgcctttag gtgcctgtaa caacagagtg atagcgccaa taacatcttc
     3001 taaatggact aaattaacac catgttcacc atcaggcgcg gtttttccgg caaagaagcg
     3061 tccgggatga cgtcccggtc cgaccagccc cgcaagacgc agaatatcga ccgaagtacc
     3121 gggtaaattg tgcagccagt cttcgagttc ttctaatact cgtccactgt tggtcaccgg
     3181 attacgcggg gtggtttctt tcaccgtgcc ttgcgcgtcg ccatagacag acgttgagct
     3241 ggtaaaaata atgcgaggaa tacgatgggc cagcgcgcta tccactaact cttgtaccgc
     3301 ttgtaaatag aactcatcgc cggggccgct acgacgtgcc ggaagcgtaa tgaccagcgc
     3361 atcggcatcc atcagggcat ccagatcgtc agaatcgcaa actaactcag gctccatgcg
     3421 aagcagatag ctatcaatgc cactcattcg ggccgcttcg acaccatctt gtgtggtttt
     3481 actcccggtg acttgccagc ctcgcgctga aagtgacatc gccagcggca tgcctaacca
     3541 ccctaacccg acaattgcga cctttttcat ccgttatctc ctgatacacc ttagatctat
     3601 aaggctacgc tagcgtatca aaactgacaa ttcattctat gaatgaatct gttcaataat
     3661 gataacgaca tgctgcaatg agcagtgaat cgtcggtaac cgcgtatacc agacggtgct
     3721 cctctgtaat gcgtcgggac cagaaacctg acaaattatg tttcaggggt tctggcttcc
     3781 ccttaccttc aaatggcgtt ctgcgggtat ctttgataag ttcattgatc tttttaacaa
     3841 ttcgcttatc tgtttcctgc cagtacagat aatcatccca tgattcctca gaccagatta
     3901 gtttcactca ataatgtcct tttctgttcc tttgcctgat ttcaggctat cgattgagtc
     3961 catcaatctc cgggcgttag cgggggagcg cagtagataa gccgtctctt ccagcgagtt
     4021 gtattcttcg agtgacatca gaacacaagc ctctccattc tgacgagtaa taaggatcgg
     4081 ggcatgatct tcaacggctt tcatcattgt tgccgacaaa ttctgacgcg cttcgctgta
     4141 gctaattgta cgcatgtcaa tctcctcttt tgtacagttt attgtacaat gatgggcgtt
     4201 aattaactat ttattaatta gtttgtagat caaggtattg tcagtgagac gaaaatccag
     4261 gcttcgctat ttttggtgcc gtcagcttag aggacagtcc tcttagcccc ctcctttccc
     4321 cgctcattca ttaaacaaat ccattgccat aaaatatata aaaaagccct tgctttctaa
     4381 cgtgaaagtg gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg
     4441 acacgcgttc aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga
     4501 tgtgtgctgg aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg
     4561 aagatcacct tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa
     4621 gaggaataac aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggccg
     4681 tttaagtgat gactcacgcg aattactggc gcgctgtggc attaaaatta atcttcacac
     4741 ccagcgcctg atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga
     4801 cgacattccc ggtctggtaa tggatggcgt ggtagacctt gggattatcg gcgaaaacgt
     4861 gctggaagaa gagctgctta accgccgcgc ccagggtgaa gatccgcgct actttacctt
     4921 gcgtcgtctg gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg
     4981 ggacggcccg ctctccttaa acggtaaacg tatcgccacc tcttatcctc acctgctcaa
     5041 gcgttatctc gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga
     5101 agtcgccccg cgtgccggac tggcggatgc gatttgcgat ctggtttcca ccggtgccac
     5161 gctggaagca aacggcctgc gcgaagtcga agttatctac cgctcgaaag cctgccttat
     5221 ccagcgcgat ggtgaaatgg aagaatccaa acagcagctg atcgacaaac tgctgacccg
     5281 tattcagggt gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga
     5341 acgtctggat gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct
     5401 ggcgggtgac caacaacgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga
     5461 aacgatggaa aaactgaaag cgctgggtgc cagttcaatt ctggtcctgc cgattgagaa
     5521 gatgatggag tgatcgccat gagctttaac acaatcattg actggaatag ctgtactgcg
     5581 gagcaacaac gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc
     5641 actgttaacg atattctcga taacgtgaaa gcacgcggtg atgacgccct gcgggaatac
     5701 agcgcgaagt ttgataaaac cacggttaac gcgctgaagg tgtctgctga ggagattgcc
     5761 gccgctagcg aacgcctgag cgacgagcta aaacaggcga tggcggtggc agtaaaaaat
     5821 attgaaacct tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc
     5881 gtgcgttgcc agcaggtcac gcgcccggtc gcttcagttg ggttgtatat tcctggcggc
     5941 tccgccccgc tcttctcaac ggtattaatg ctggcgactc cggcgcgtat tgcgggctgt
     6001 aaaaaagtgg tgctgtgctc accgccgccg attgccgatg agatccttta tgcggcgcag
     6061 ctgtgcggtg tgaaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcgctggcg
     6121 tttggtacgg aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc
     6181 accgaagcga aacgtcaggt gagccagcgt ctggacggtg cggctatcga tatgcccgca
     6241 ggcccgtcgg aagtgttggt cattgccgac agcggcgcaa cgccggattt cgtggcttct
     6301 gatttgcttt ctcaggctga acacggcccg gactcacagg tgattttact gacgcctgac
     6361 gctgatatgg cgcatcaagt tgccgaagcc gtcgaacgcc agttagcaga actgccgcgt
     6421 gccgaaaccg cccgccaggc actgaacgcc agccgcctga tcgtgactaa agatttagcg
     6481 cagtgcgtag agatctccaa ccagtacggc ccggagcacc tgatcatcca gacccgcaac
     6541 gcccgcgatc tggtcgatgg catcaccagc gccggttcgg tatttcttgg tgactggtca
     6601 ccggaatcgg caggtgatta cgcctccggc accaaccacg ttctgccgac ttacggttac
     6661 accgccacct gttccagcct cgggctggca gatttccaga agcgtatgac cgtgcaggaa
     6721 ctgtcgaaag aaggtttctc cgcgctggct tcgaccattg aaacattggc cgccgccgag
     6781 cgcctgaccg cccataaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca
     6841 tgagcaccgt gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc
     6901 agtcggcgcg tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatacccca
     6961 ctgccgtgga gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgtcagccga
     7021 aagcggtgat tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca
     7081 gtcgtggcgc ggacgaaggt attgaactgc tgattcgcgc tttttgcgaa tcgggtaaag
     7141 acgccatcct ctactgcccg ccaacgtacg gcatgtacag cgtcagcgcc gaaacgattg
     7201 gcgtcgagtg ccgcacagtg ccgacgcagg acaactggca actggactta cagggcattt
     7261 ccgacaagct ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccgacagggc
     7321 aactgatcaa tccgcaggat tttcgcactc tgctggagct aacccgcgga aaagccattg
     7381 tggttgccga tgaagcctat atcgagtttt gcccgcaggc atcgctggct ggctggctga
     7441 cggaatatcc gcacctggct attttgcgca cactgtcgaa agcttttgct ctggcgggcc
     7501 ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg
     7561 ccccctaccc gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg
     7621 gaatcgtcgc tatgcgcgaa cgagtgacgc aaattattgc agaacgcgaa tacctgattg
     7681 ccgcactgaa agagatcccc tgcgtagagc aggttttcga ctccgcaacc aactacattc
     7741 tggcgcgctt taaagcctcc agcgcagtgt ttaaatcttt gtgggatcag ggcattatct
     7801 tacgtgatca gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc
     7861 gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa
     7921 gtatcttttt atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt
     7981 ggaccgtttt gacaaactcg cctttgaacc aggcgtgatc ccggaactgc tgaagctgca
     8041 aaaagcgggc tacaaactgg taatgatcac caatcaggat ggtctgggaa cacaaagttt
     8101 cccgcaggcg gatttcgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg
     8161 cgtacagttt gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt gcgactgccg
     8221 taagccgaaa gtaaaactgg tggaacgtta tctggctgag caagcgatgg atcgcgctaa
     8281 cagttatgtg attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa
     8341 tggtttacgc tacgaccgcg aaatcctgaa ctggccaatg attggcgagc agctcactaa
     8401 acgagaccgt tacgctcacg tagtgcgcaa caccaaagag acacagattg acgttcaggt
     8461 gtggctggat cgcgaaggtg gcagcaagat taacaccggt gttggcttct ttgatcacat
     8521 gctggatcag atcgccaccc acggtggttt ccgtatggaa atcaacgtca aaggcgacct
     8581 ctatatcgac gatcaccaca ccgtcgaaga taccggcctg gcgctgggcg aagcgttaaa
     8641 aatggctctt ggcgacaaac gcggtatttg ccgctttggt tttgtgctac cgatggacga
     8701 atgccttgcc cgctgcgcgc tggatatctc tggtcgcccg cacctggaat acaaagccga
     8761 gtttacctac cagcgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc
     8821 gctctcatac accatgggcg tgacgctaca cctgaaaacc aaaggtaaaa acgatcacca
     8881 ccgtgtagag agcctgttca aagcctttgg tcgcaccctg cgccaagcca tccgcgtgga
     8941 aggcgacacc ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatacc
     9001 ggctgcgcca atctgaactc ggtgaagtca gccattgcgc gccacggtta tgaaccaaaa
     9061 gtcagccgtg acccggacgt ggtgttgctg gccgataaac tatttttacc cggcgttggc
     9121 actgcgcaag cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc
     9181 tgtacccaac cggtactggg catctgctta gggatgcaac tgctgggtcg gcgcagcgaa
     9241 gagagcaacg gcgtcgactt gctgggcatc attgacgaag acgtgccgaa aatgaccgac
     9301 tttggtctgc cgctgccgca tatgggctgg aatcgcgttt acccgcaggc aggcaaccgc
     9361 ctgtttcagg ggattgaaga cggcgcgtac ttttactttg ttcacagtta cgcaatgccg
     9421 gtcaatccgt ggaccattgc ccaatgtaat tacggcgaac cgttcaccgc ggcggtacaa
     9481 aaagataact tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag
     9541 ttgctgaaaa acttcctgga gatgtgatga ttattccggc attagattta atcgacggca
     9601 ccgtggtgcg tctccatcag ggcgattacg gcaaacaacg cgattacggt aacgacccgc
     9661 tgccgcgctt acaggattac gccgcgcagg gtgccgaagt gctgcatctg gtggatctga
     9721 ccggggcaaa agatccggca aaacgtcaaa tcccgctgat taaaaccctg gtcgcgggcg
     9781 ttaacgttcc ggtgcaggtt ggcggcggtg tgcgtagcga agaagacgtg gcggcgttac
     9841 tggaagcggg cgttgcgcgc gtggtggttg gctccaccgc ggtgaaatca ccagaaaggg
     9901 tgaaaggctg gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta
     9961 ttgacgagca aggcaacaag caggtggcgg tcagcggctg gcaagagaac tcgggcgttt
    10021 cactggaaca actggtggaa acctatctgc ctgtcggcct gaaacatgtg ctgtgtaccg
    10081 atatctcgcg cgacggcacg ctggcaggct ctaacgtctc tttatatgaa gaagtgtgcg
    10141 ccagataccc gcaggtggca tttcagtcct ccggcggtat tggcgacatt aacgatgttg
    10201 ctgccctgcg aggcactggc gtgcgcggcg taatagttgg tcgtgcatta ctggaaggta
    10261 aattcaccgt gaaggaggcc atcgcatgct ggcaaaacgc ataatcccat gtctcgacgt
    10321 tcgtgatggt caggtggtga aaggcgtaca gtttcgcaac catgaaatta ttggcgatat
    10381 tgtaccgctg gcaaaacgct acgccgaaga aggtgcagac gaactggtgt tctacgatat
    10441 caccgcttcc agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt
    10501 gatcgacatt ccgttctgcg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat
    10561 tctttccttt ggcgcggata aaatttccat caactcccct gcgctggcag acccaacctt
    10621 aattacccgc ctggcggatc gctttggcgt gcaatgcatt gtggtcggca ttgatacctg
    10681 gtacgacggc gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac
    10741 ccgcgtcact cagtgggaaa cactcgactg ggtacaggaa gtgcaaaaac gtggagccgg
    10801 agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt aacggttacg atctcgaaca
    10861 actgaaaaaa gtgcgtgaag tttgccacgt cccgctaatt gcctccggtg gcgcgggcac
    10921 catggaacac ttcctcgaag ccttccgcga tgccgacgtt gacggcgcgc tggctgcttc
    10981 cgtattccac aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg
    11041 cgtggagatc aggatatgtt aacagaacaa caacgccgcg aactggactg ggaaaaaacc
    11101 gacggactaa tgccggtaat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc
    11161 tatatgaacc cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg
    11221 cgcactaaac agcgactgtg gaccaaaggc gaaacgtcgg gcaatttcct caacgtagta
    11281 aatattgcgc cggactgcga caacgacacg ctactggtac tggcgaatcc tatcggacca
    11341 acctgccaca aaggcaccag cagctgcttc ggcgacaccg ctcaccaatg gctgttcctg
    11401 tatcaactgg aacaactgct cgccgaacgc aaatctgccg acccggaaac ctcctatacc
    11461 gccaaactgt atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgta
    11521 gaaaccgcac tggcagcgac ggtacatgac cgctttgagc tgaccaacga ggcatcggac
    11581 ttgatgtatc acctgctggt gctgttgcag gatcaggatc tggatttaac gacggtaatt
    11641 gagaacctgc gtaaacggca tcagtgagtt gcagggtaag cggatgcgat gttgttgtcg
    11701 catccggcaa aaaaaaaggg caaggtgtca ccaccctgcc cgttttcttt aaagctgcaa
    11761 agattacttc gcgttataat tacgcagagc gttacgcccc agcacaatcc ccgcgccaac
    11821 catgccgccc agcagcaccg ccagaatcaa ggtaattgcc tttttcgggc tatcgcgacg
    11881 aataggtaac gtcggtttca tcacataacg gtaagcatga atatcaagat catcaacctt
    11941 taagttttca atatccaaca ggttttgacg tgtctgatag tagtttggtg agaacaccaa
    12001 cggacgggtc gcctcatgct taatcatcga ctccagcgct tcgctcccca aaaggaacaa
    12061 cgtatcttgt gtcacatctt cagtctgctg aacctgtggc tttgccacct gcgcctgatt
    12121 cgcatactgc aacgcttcct gaatctgacg gatacgcaga tctttctgct cctgcgcgac
    12181 tacttcctgc gttcttaaag agtcctgcaa gtttttccgt cccagagcaa tgttgtcttt
    12241 gagatccttt tctagctctt gattcacttt atcatcaact tgctgaatgt attgggccaa
    12301 cttcatttgt gctccctctg cagtttgccc aacataagaa acagtcaatg gtaattgctg
    12361 gttcttaaca gaaggttcga tggtaagttt ttctggttct tcctgattat ccagcgtttc
    12421 tgctaatgca gagaaggcag aactgaagcg accaattaac gtctcctgta aatccgatac
    12481 tttcggtgca gcctgaccat agataacatt catggcattg ttatagccag caatttgccc
    12541 cacgtcgggc tgagtgataa ttgctgttga cgtccatttc tccttcgcta ctgccaaata
    12601 tccaatagcc agggcaatag ccacaatgac ggaaatgatg attgtcatct tgccacgcca
    12661 caactgcgct agtaaatcaa tcaaatcaat ctgttccggg tcatggtttt gcccagaaac
    12721 attattattt tctactctca tcattaccct aactgacgga aatttccaag tccgcatagt
    12781 ttagcgataa tcctagtgaa tgctatagga taagtgataa aaataatgaa ttattgggga
    12841 ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac aagatgttag tcgctgccaa
    12901 agagatcgcg ggtgtatacc ttatctgcca catctttaag ctcttctgcc attcggttag
    12961 agataatgac gtcggcttgt tgtttgaaga tggcgagatc acgttccagc cgagagttga
    13021 agaatgagtc ttctttcatc actggctcgt agatgatcac ttcaacgcct ttcgccttga
    13081 tacgcttcat aatcccctga atagaagacg cacggaagtt atctgaaccg ctcttcataa
    13141 tcagacgata aatacccacc acttgcggtt tgcgtgacaa aatggcatcg gcaataaaat
    13201 ccttacgcgt gcggtttgca tcgacaattg ccgagatcag gttattcggc acagactgat
    13261 agttcgccag taactgcttg gtatctttcg gcaagcaata accaccataa ccaaacgacg
    13321 ggttgttgta atggttgcca atacgcggat cgagacaaac gccttcgata atctggcgag
    13381 tattcagacc taaactttct gcgtagctat ccagttcgtt aaagtatgcc acgcgcatcg
    13441 ctaggtaggt gtttgcaaaa agtttaatcg cttctgcttc ggtcgaatcg gtgaacaggg
    13501 tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc agcaaaacgt tctgcgcgtt
    13561 ctgaacgctc accgatgaca atacgtgaag gatggagatt atcgtaaagg gctttacctt
    13621 cacggagaaa ttccggagag aaaataatat tttcagtacg atatttctta tgcatcgctt
    13681 cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataagga tttatctcaa
    13741 ctacgtcttt aattactgat tctacactgg atgtattgaa ataattagtt ttaggatcat
    13801 agtcggttgg agtggcgatg atgacataat cagcatcccg gtaagcttca tttttatcta
    13861 atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa
    13921 taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa
    13981 cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc tacatagcca gtaccggaaa
    14041 tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc
    14101 atcctgataa gagctaaatg ttttaacgct acgttattag ctggtcaacg ctacgaaaaa
    14161 caattcagat tttctttata agaatagctg atatttatta agttaatatt aagcagtgaa
    14221 atttagtcta taagataaat ggcaaaaaaa aagcccggtg caatatacgc cgggcctcaa
    14281 ttttactgtt ggttaaatca gattaatcca gccattcagt atggaacaca ccttctttat
    14341 caatgcgctt ataagtatgt gcaccgaaat agtcacgctg tgcctggatc aggttcgcag
    14401 gcagaacagc ggcacggtag ctatcgtaat aggcaaccgc agcggcgaag gtcggaaccg
    14461 ggataccgtt ctgtactgca taagcaacga catcacgcag cgcctgctgg tagtcatcgg
    14521 caatttgctt gaagtacgga gccagcagca ggttagcgat ctgcggattt tcggcatatg
    14581 catcggtgat tttctgcagg aactgcgcac ggatgatgca gccagcacgg aaaatcttcg
    14641 cgatttcgcc gtagttcaga tcccagttgt attcttcaga cgcagcacgc agctgagaga
    14701 agccctgagc gtaagaaacg attttgccca gatacagcgc acggcgaact ttttcgatga
    14761 actcagcctt gtcgcctgct gactgagctt gcggaccaga gagaacttta gatgcggcaa
    14821 cgcgctgatc tttcagagaa gagatataac gtgcaaacac agactcggta atcagcgaca
    14881 gcggttctcc gagatccagc gcgctctggc tggtccattt accggtacct ttgttagccg
    14941 cttcatccag gatcacatca accaggtagt taccgtcttc atctttttta gtgaagatat
    15001 ctttggtgat gtcgatcagg tagctgctca gttcaccgtt attccactcg gtaaaggtct
    15061 gtgccagttc ttcgttagag agattcaggc cgcctttcag gagagaatag gcttcagcaa
    15121 tcagttgcat atcaccgtat tcaataccgt tgtgaaccat cttcacataa tggcctgcgc
    15181 catcagcacc gatataggta acgcacggtt cgccatcttc agcaacggcg gcgattttgg
    15241 tcaggatcgg cgcaaccagt tcataggctt ctttctggcc accaggcatg atggacggcc
    15301 ctttcagcgc cccctcttct ccgccggaaa caccggtacc gatgaagtta aagccttcgg
    15361 cagaaagctc acggttacga cgaatggtgt cctggaagaa ggtgttacca ccatcgatga
    15421 tgatgtcgcc tttatcgaga tatggtttga gcgaatcaat agcagcatcc gtgcctgcac
    15481 ctgctttcac cattaacagg atgcgacgag gcgtttccag agattcaaca aactctttca
    15541 ccgtatagta aggaaccagt ttcttgcctg gattttcggc aatcacttct tccgtctttt
    15601 cacgggaacg gttgaaaata gagacggtat aaccacggct ttcgatgttg agcgcaaggt
    15661 tgcgccccat cactgccata ccgactacgc cgatctgttg ctttgacatt gtttactcct
    15721 gtcagggggt ggtcaccgcc gagtgtgcgg cttaaatgtg gtatagatgt taactcactc
    15781 atagagatgg tgaatagcat tacctttgct ataccacact gaatatctca ccttttccgt
    15841 cttttaaaac ttatttatat gaaattactg aattacttgt tcagtaactc aagaataagt
    15901 tttgtctttt cttccatcag cggcacatca ccgcgagatt ccacattcaa ccgcaccacc
    15961 ggttcggtgt tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca
    16021 tcggtgcgat ccaccgccag cgcctcgcgg ctaaaatgct gttccacgcg gttaatcgcc
    16081 tcaaagggtt gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc tgccatccgg
    16141 tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc
    16201 cacgggatca tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt
    16261 tcaccaccgt agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt
    16321 ttcgacatca ccggcgtgcc gcctgcggca gtcaccacat caacggtgtt ccaggagaga
    16381 cgcggatcgt ggatgatctt cgcgccggga tttttttcga ggaatgcttc tgccagcagg
    16441 ccgacaatgt agtagccctc aataaactgc cctttttcgt caaacaggaa gcagcggtca
    16501 aaatcgccat caaaagcaat gcccatatcc gcaccgtgtt tgatgaccgc attgcgggtg
    16561 tcgtcgcggc attccggcag tagcgggtta ggaataccgt tggggaaatt gccgtccggc
    16621 gtgttgtgca ctttgattaa ttccacgggt gcgccaaggg ctttaaagcg ggcttcgatg
    16681 gcgtccacca ccggacccgc tgcgccattc ccggagttaa tcaccagctt gagcggcgtg
    16741 aggtttttga cattgatata accgaacagg tgatcaacgt aagcgtcacg cagattgatt
    16801 tgctgatagc gaccgcgttt ggtttcatca acgggaggaa agtcgttggc ttctgccaga
    16861 cgctggacat cgcgcagtcc ggtatcaccg ctgatcgggc gagccccttc gcgcaccagt
    16921 ttcatgccgt tgtaatccat cgggttatgg ctggcggtaa cttcaatgcc gccatccacg
    16981 ccgagatgga acgtggcgaa atagatctct tcggtgccgg acatgccaat atccagcaca
    17041 tcgacgcccg catcctgtaa acctctcgcc agcgccagtt ttaaggtttc gctggtgagg
    17101 cggacatcac cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca
    17161 atgcgccagg cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct
    17221 ttaaagcagg ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc
    17281 gaaacggcca attttgtttt tgtcgttatt tacatctcgg tttcgcattc attgcctgat
    17341 gcgacgttta cacccgtccg tagcgatccg cgaaacgcac cacatcatcc tcttcgagat
    17401 aagagccgga gcgcacttca attaaatcga gcggaatttt ccccgggttt tccaggcaat
    17461 gcatcgcccc cagcggaata taaatggact cgttttcacc aagcagtttg atatcaccat
    17521 caatggtgac ttttgccgtt cccgcgacaa ccacccagtg ttccgcgcgg tgatggtgca
    17581 tctgtaccga caagccctcg cccggtttca cggtgatgcg tttcacctgg tagcggtcgc
    17641 ccgcgtcgat agagtcatat ttgccccacg ggcgatagac ttcgcgatgc acccgatgct
    17701 catggcgacc atcggctttg atctgctcga ccactttttt cacatcctgc accgcgttac
    17761 ggtcggcaat cagcaccgca tctttggtct gcaccactac cagatctttc accccgacgg
    17821 tggttacgag gccagattcg gcgtacacat agctgttttc agttttgtga ttaatcacat
    17881 cgccgtggca aacgttgccc tcggcggtgt gggcgctaat ttcccataat gaggaccagg
    17941 agccgacatc gctccagccc gcatccatcg gcaccacaac ggcatctgcc gtacgttcca
    18001 tgaccgcgta atccaccgac tcttccgggc aggcgagaaa cgcttcttca tccacacgaa
    18061 taaaatcgag atccggatcg acggcgctca tcgctttttc acaggcatcg agaatatccg
    18121 gacgatattt tttcagttct tcgagatagc gtccggcgcg gaacaggaac atgccgctgt
    18181 tccagtaata ttcgccgctt gccacatagg cctgggcggt ttccagattc ggtttttcga
    18241 caaactgcgc cacttcaaag gccaccgtat cctgctcacc cgcagacact tcaccgcgac
    18301 gaatatagcc ataaccggtt tctggtagat ccggcacaat gccgaaggtc accagcttgc
    18361 ccgcttcggc atatggcatg gcattacgca cggcggcacg gaacgcgtct tcatcggcaa
    18421 tcacatgatc cgccgccaat accagcatta acgggtcgct ctccgggcta tgacgttttg
    18481 ccgccagcgc cgccagcgca atggcaggtg ccgtgtttcg ccctgccggt tcgagaataa
    18541 tgttctcggt aagtttgttc agttgacgca gctgttccgc gacaataaag cggtgctgct
    18601 cattgcaaat caccaccggg ctttcgcact ccacgccgtt caggcggcag atggtggttt
    18661 gcagcatggt gagatcgcct ttcaggcata aaaactgctt ggggtaaagt acgcgggaaa
    18721 gcggccataa gcggctaccg gagccacctg ccatcacaac tggatagagt ttcgactgcg
    18781 ccataattat ccccgaatat catttataaa ttgacgtaac acgttctctt tatcgagtgt
    18841 gcgttcggca tattcacgtg ccaccgtgtt gtgtttgggc agcaggagcg cctgacggat
    18901 ccccgccacc agcgcctcga ccgattccgg ttctacgcaa accgcaatgc ccggaaaggt
    18961 ttcgcaaagc tgccccagtt ctgtgtgggc ctctgcggta atcaccgcgt taccgcctac
    19021 cgccagaata ttggtcagtt tcgacggcaa tacggcatct gccgcgccgc gtttttgcac
    19081 caccagatgg caatcgccca tcttcagcag tgcgggtaaa gcgtcatacg attgcagcgg
    19141 gaaaaattgc atgttgcgca gtccacgctg ctgcgccatt ttttccagcc gcgctttgcc
    19201 gccgccctgc ccgacaatgg caaaaatcag cggttcatcg cgcagacgat cggcagcttc
    19261 aataacgttt tccagcccct gcttttcacc aatattgccg gagtaaagaa tgattttttt
    19321 gttatccggc aggtcaagct ggttacgaag ggcgtcaaca tcggcatctg caacatgctg
    19381 aaaacgggca atttccgacc agttggggaa gaagatgacg ttttccgccg ccacgccttt
    19441 ttcgatggct ttattcatca tcgaacgcga aatcgtggag acgttatcga cgttatgcag
    19501 tccgctacgt tcgaacgccg ttgccagctg tgccactttg ctgcctttgc cttttccggc
    19561 aaggcccagc cccagcatgg cgtccacttc gtaatcctga atatgcagca cggtacgcgc
    19621 accagaaagt ttcgccagca ggcgcattcc cggcgtgcaa aacagcgttg gcaccacgcc
    19681 gataatgcga tccggcttcc agcgacgttg cgccatcaga ggaaagaaac tgctgacggc
    19741 aaaactgccg agatgtaaca ggcgtttcag ggtgctcggc tgttttggca cgtacagcgg
    19801 gcagcgccac accgtggcgg ccccctcttc tcgtttgtac cgccaggcgg aatagttctc
    19861 gcccacctgc cactgcgggt agtaaggcgg tgcggtaata acccgcacct catgaccttg
    19921 tgccgccagc cattccacca tctcgccggt gtatttgccg ataccggtta actccggcga
    19981 gtagttaatg ccgtagacca gtattttcat aatccgggta ctccggcacg cttctcagcg
    20041 agaaaatagg cgcggctgtt agcgtgaaca tcgttactgg cgagcagcgc gtccggcgtc
    20101 agccagcggt aatcgtcatg ctgctcatcc ggcagtatca gctcttcttc agcgactctg
    20161 aagcgaaaac cgagcaccac atagtgagtg gtgaaatccg tgccggagaa gttatcgtca
    20221 taaaagtgct gccagacacc gtaaaactgg cctgctgtta tcggcagacg cagccccagt
    20281 tccgccatcg tcagccgctc gaatgcggct tccagcgttt cgtctttctg cacgcgccct
    20341 cccggcacaa accagtaacc ctgcgccggg cggttggttc ttttgccaag cagaaactca
    20401 ccgcgactgt tctcgacaat aaagtcgaga gagacaagcg gggtggagcg cactaccgtg
    20461 gcaaagtctt cctgacgtaa aaacatcatt acccccgaaa gcggtcttga ttctcaagga
    20521 accactggta agtgctggca agccccgctt ccagtgagat ttcgtgatac cagccaagct
    20581 gatgcaggcg cgtcacatcc agcagtttgc gcggcgtacc atccggtttg ctggcatcaa
    20641 aaaccacccg acctttgtaa cccaccactt tggcgatggt ttgcgccagc tcgcggatag
    20701 tgcagtcaac gcccgtgccg acgttaatgt gcgacagcat cggctgggtg ttctccagcc
    20761 agacttcgtg cgccagctcc atgacatgaa tgctcgccgc cgccatatca tcgacgtgca
    20821 ggaattcacg catcggtgta ccgctgcccc ataccaccac gtccggtgca ttctgtgccg
    20881 tcgcctcgtg gaagcgacgc agcaatgcag ggatcacatg cgaattactc gggtggaagt
    20941 tgtcatgcgg gccatacagg ttggtcggca tgactgagcg gtaatcgcgt ccgtactggc
    21001 ggttgtatga ttcgcacagt ttgatcccgg cgattttggc aatggcgtaa ggctcgttgg
    21061 tcggctccag cgtgccctgc aataactcgc tttctgccat cggctgtttt gccagtttcg
    21121 ggtagataca ggacgatcca agaaacagca gtttgttcac gtcgttctga tgcgcggcgt
    21181 gaatgatgtt gctctcaatc atcatgttct ggtagatgaa atccgccgga taggtgttgt
    21241 tggcaacaat gccgcccact ttcgccgccg ccagatagac ctggtcaatg cgttcgctgg
    21301 caaagaaatc atgcaccgcg cggctgtcca gcaggttcag ctcgtcgcgg gtgcgtaata
    21361 ccagttccac atcaccgcgc tgttcgagct gccgcctgat ggcagaaccg accatcccac
    21421 gatggccagc gataaaaatg cgttgtttac tcatgcttat gactccagcg cgatcgccac
    21481 gtcgtagccg tgagatttca gcagagagtg ttttttcgcc gcttcgaggt cattagccac
    21541 catttcagac accatctctc tgagggtgat ttccggtttc cagcccagtt tttcgtgcgc
    21601 tttggtcggg tcgccgagca gcgtttcaac ttcagccgga cggaagtaac gcggatcaac
    21661 ggcgataatc acatcacccg gtttaacgcc cggcgcgtca tgcccggtga cggaaaccac
    21721 aatgcccttc tcttcaacac ccgtgccttc aaagcgcagt ttgatgccca gctgtgccgc
    21781 tgccatttcc acgaactgac gcacggagta ctgaacgccg gtcgcgataa cgaaatcttc
    21841 cggctgttcc tgttgcagca tcatccactg catttttacg tagtctttgg cgtggcccca
    21901 gtcacgcagg gaatccatat tgccgaggta caggcacgac tccagcccct gggcgatgtt
    21961 ggcgattgcg cgggtgattt tgcgggtaac gaaggtttcg ccgcggcgcg gggattcatg
    22021 gttgaagaga attccgttac aagcgtacat gccgtaggat tcgcggtagt taacggtgat
    22081 ccagtaggcg tacagtttgg cgaccgcata cggagatcgc gggtagaacg gcgtggtttc
    22141 tttctgcgga atttcctgca ccagaccata cagttcagag gtggaagcct gatagaaacg
    22201 agttttcttt tccagaccga ggaagcggat cgcctccagc aggcgcagcg tacccatcgc
    22261 gtcgacgtca gcggtatatt ctggtgactc aaaagagacc gcaacgtggc tcattgcgcc
    22321 caggttgtac acttcatccg gctgtacttc acgcaaaatg cgcgtcaggt tagaggtatc
    22381 actcaggtcg ccataatgca gatggaattt cgggttgcag gtgtgcggat cctgataaat
    22441 gtgatccacg cgctcggtgt tgaatgacga tgcgcgacgc ttaataccat gcacctcgta
    22501 acctttttcc agcagaaact ctgccaggta agaaccgtct tgtccggtta caccggtgat
    22561 gagagcgact tttgacatgt cttattcctc tgtatttttg aatttattca gtttcaacgc
    22621 gttcgcgtat caccactgcg ggattccccc ggcaaaccac atttgccgga agcgatttaa
    22681 aaacactgct tcgtgcaccc acgacggtgc cgtcgccgat tgtgacgcct ggggcaacaa
    22741 agacatcggt tgccagccag catttctcgc caatcacaat aggcgtggcg ttaatggtga
    22801 aatgttgact tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg
    22861 aatgtgcgcc aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccgacccacg
    22921 cgtaatcacc taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta
    22981 cgttttttcc tatttttgct ccgaataaac gtaataaaaa agcccgccag cgatacaata
    23041 cttgtggcga ccaggcaaat attgttgcct gtactgccca ccataattgc actttaatag
    23101 cgttgccgcc ccggaaccct ttcggcaccg agaaaccgct taaatcttgc attggttttc
    23161 cttatattca gactttgtta tataaggctt tcgtctttga ggtagtacgt tggcgtaaat
    23221 gccaggataa ttcagcccaa aagccaggca catgtaatat ttgtcgttgg acttttttcg
    23281 cgtcagcaca caattccata ttattggtgg tagatacccc acccatggaa aattcagaca
    23341 ccaggccatt gagtttttta aatgcataac cggctttata cattttggct gccagcgcgt
    23401 agtcggaaga aactttatat tccaggtcat aacgccattt tttcaagccg gatacaggga
    23461 aaaatatcgc ctgatgactg gcgggcaggc tgtgataaat ataccagccc ggtttggcgc
    23521 tacgtttaat tttatgcccg tcgccaaaat ccagcagcgc atcgccggtg atcatcatgt
    23581 tatctttttg catttttaac ttacggacaa aatttgcggc atcctgatga aaaatatcgc
    23641 ccgaattgag aaacaacgcg aacttgcctt gtgccatcgt aatgcctttg ttcatggcgt
    23701 cgtagatacc gttatctggc tcgctgacaa agcgtaggtt atagatacca ttgagatttt
    23761 ccagatactc acgggtgccg tcattggaac cgccatcgac aacaatccat tcgaagctga
    23821 tatcttccgc ctgcgccaga tgcgccagcg aggcatgtgt tttgactatc ccttcgaggt
    23881 tacgaaacgc gacagtgatt atgctaagca acatgttctt attcttacct cgtaatattt
    23941 aacgcttttc gcaaaataaa cggacagacg attaaaaatg catattccgg gctaaatatc
    24001 gaaccggtaa aaaacagcga taccggagta aaaagatata attgcacgcg aaaattgcgg
    24061 ttatcaccaa aagcgttgat catcattttt atcactttcc ccatgtacca cagcgataaa
    24121 aacactgcga accaggaaaa ataaataatc agcagataca aaccattgtc tatggttttt
    24181 ccgacatccg caccgttaaa tattccgaat gatgcgacgt attcataaag tgagccaaat
    24241 ctgactacac cgtcaatatg ggtcaaagaa taaccgacca tcaccagcgg accgacaata
    24301 cgataatacg atgacgatcc ttccgtacct aaatcgccca gacgggtgga aatataagga
    24361 aacgcgatta ccacaccaac ccggaataca gccagagaaa tcaatgccaa cggtaacttt
    24421 ttcttaatcg cctctttatt cagatattga aatgcccact ccagcaaata aaacaggata
    24481 aaagtcataa cccctgaaaa cgatccggat aatattatcc ctgcgagaat catagcatcg
    24541 gttttaggcg ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga
    24601 gcgaaaaatg ccggttcgaa ataaagtgct ttcgtgcgct tgccgccgaa tttaatgaaa
    24661 ttcagtacat agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg
    24721 ccaccggtga ggataatttg cgccatctcc accgccgcca gcgccaccac cagcccgatc
    24781 accagataaa agaaacgtaa tatcttgcga tggttgtgtg gcgaaatcgt tttaaagcga
    24841 atactccaca ccatgccaat aatgatcaca atatagacaa acagcatggt tgaagtgacg
    24901 tatttactgg catccagcga ctgaccaaac agatagttaa acgccgtgag tcccgcacca
    24961 atccctaagg caatcatcaa ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag
    25021 acaggtaaaa aagtgacgat ggtgatgggg aaactttcgc caagctgggc gattttgacg
    25081 ttaaccagca ggtagatcag cggcagcagc aggtagctac agattctgat agaagttgac
    25141 atactcctcc agcatctgtt gtccactgta ggtggcgcgg ctgcgttggc tgaactcagc
    25201 cagcgtggta ccaaatatcg cctgcgcgat ttccggtttg cttaactgca ccagttgcag
    25261 cacgtcttct tcgtcgacgg ttttaccgcc ggatttttgc aacacttccc gtgccgcgtc
    25321 gctatgggtg gcaatcaccg gcacgccaat tgataacgcc tcacacaaaa tcagcgggta
    25381 gttatcgacg cgagaactga ataccagcgc atccatctga ttgagcgcgc tcatcaactt
    25441 gcgcttgtca gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt
    25501 accaaaggta tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg
    25561 gttagtcttg ccgtcgtaac gcaagtcatg cgccaccacc gcgattttcg gcttgccctg
    25621 ggtttcgcgc accggaggca agtccgccag aatcgcttcg gttgccatat caataccatt
    25681 attgataatc cggcaacgcc ctggaccgta caggctattg aaagcgtcag ccacatgctg
    25741 gctgggggaa ataaactgac agcccagcgc cagcatctca cggaataact ggcgtttgcc
    25801 cgccaccagc tggtgtgcgc gatcaatctt caccggcgga tagttattta aggtcgggca
    25861 tttctggcag cccgttttcc agccttcgca accgtcggta aaggcgcagc gtccggtaac
    25921 actccagtgg tcgtgcagcg tccagaccag agtgacgtcc ggtttatggt ttttcacttt
    25981 ttcgcaaaag cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg
    26041 caggaccacc ggacccggtg tgcgagtaat ggtgcgatat aactcattga aattgccaaa
    26101 cagatcgcga ttaaacagac gaaacagggc aatattcgcc atcgcggtca tccgcggcgt
    26161 atgtttgatg acctgcggat agttctgatg gctgacgctc tctttgccgc ctttgccata
    26221 accgtagaca aaatgtgacg ccagcccctg ttgcagcgca cgctggtgga gatctaacgc
    26281 cacacctgct gccccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt
    26341 accttcactc gcgctttttc tcccaccacc agcgcgttgt ccgggacaga gtcgagcacc
    26401 acgctgcccg cacccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg
    26461 ttggcaccga gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga
    26521 ttgccgatag tgacgccgtg gcgaatggtg aaatcatccc ccgctaccac gtttttattg
    26581 atcactacgg cgtaaccgtg atggatagta aagcggcggc caatggtcgc ggcggcctgg
    26641 atttcataac cgaaaaagca ttcggtgata atgcgataca gcaccagcag cggggccgcc
    26701 cacagattgt tgaggacgtt ctttttgcgc cacaccgaac aaaaatgagc aacacgatag
    26761 gcaagaacca tgcagcacgg gcgtaaactc cagctgttgg cacgcagatc ttccagcatc
    26821 tttagcgccc ccgaatacca tcagccagac gtttgccgtt acgcaccgac agcagcgtca
    26881 gcaaagtgcg ccaggtcata cgtttattgc ggatctggta gagggtaaac agctgatatt
    26941 ttttgcttgc acggtcaaat ttatctttgt gctttcggta aaagtggaag tacccggaga
    27001 attttttcgg tgacgaggtg atctgcattt caccgtgatt gatatgcagg atctgcgtcg
    27061 cttcttccac tttccacggt tcgccgtact ccaccaccat ccgcaggaag atgtcgtaat
    27121 cctgtgctgc tttcagttcg gtatcgaaca aacactcttt gaaacgccat gcccaagtaa
    27181 agacctgatt gccaatgata ttgcgtttat agaacaggcg gcgtgagtac ggcgatttgg
    27241 gatacagtgg taagcttgcc ggttgggaat aaacttctcc ctggcatacg tagtcgttag
    27301 cgtacaaaaa ggcgtgagtg accagttgct gtttatgggc gaggaagacg ctcagacggt
    27361 tgggtgtcca ttcgtcatcg tcatcgatcc cggtaatgta ttccccttgc gccagcataa
    27421 tcgcctggtt acgtaccgcg cacgccccgc tgttaatgtc gttgtgaatg taggtgatac
    27481 gcggatcgtt gagggcagtg acgtactgtt gcagttgttc ccaggaggtg gagcaatcat
    27541 ccacgatgat catctcccag ttgctgtagt cctggcgcag gaccgatttt atcgcccgaa
    27601 tcgccagttg ttgacggttc caggtcggca tatagattga aatcagcgga ttctctttca
    27661 taggttgttc tcccggctta tgagctggtt tgcttgccgg atgcggcgtg agcgccttat
    27721 ccggcctacg gggcggtgcg aatgcaggcg tcgttatttc gcatccgact tatattcgta
    27781 ttcgtaatag ccataatcct gatacgcgct ggcgcggcgg aagatggagt tcagaatcac
    27841 ccctttcacc ggaataccgt tttgctcaaa gcggctcaga ctggtttcca cttctttcaa
    27901 tgtgttgacc gcatagcgcg ccaccattaa cgtggttcca gcgtgacgcc cgacaattgc
    27961 tgcatcggtc actgccagaa tcggcggcgt atcaatcaac accaggtcgt agtttttact
    28021 cgcccagctc accaattcgg caaagcgttc gctcatcaac agttcagaag gatttggcgg
    28081 cacctgaccg cgcgggatca ggtcaaattt ggcaatagag gtcggtttag ctgcggtagt
    28141 aatatcgccc tggccaatca gaatttccga caggccattg acgttattgg tacccaacag
    28201 ctcgtgggtg tagcctttgc gcatatcgca gtcgatcaac aacacccgtt tattggtctg
    28261 actgatcacc gccgccaggt tggcgcagac aaaggtttta ccgattgacg ggctcacccc
    28321 tgtcatcatc aacacattgt tctgcgcctg catcatcgcg aagtgcagac tggtacgcag
    28381 actgcggatg gcttcaatcg ccagatcggt tggattcccc accgccagta gctggctctg
    28441 tttatagcgt ttaatccctt tgatggtttt gacgctatcg cgcgctttct gccattccga
    28501 cagcgggatg ctggcataga cgctgatacc atgttcttcc agcacctgcg ggctttcgat
    28561 gccgcgatta aacaacgagc gcagcagcac acccacgata gagagcatca ggccgaggat
    28621 aattgcgccg aggataatca gtcctttctt cggtttcagc acgccaggct gggtgattgc
    28681 cgggtcaaca atgcgcacat cgccgacggt gctggcttcg gtgattttca gctcctgctc
    28741 tttattcagc agttgcatat agacctgctg accagactcg acatcgcggg tcaggcgcac
    28801 aatctcctgc tgggttttcg gcatcgccgt tacgcgaccg ttaagtttgg ctttctcttc
    28861 ttccagcgcc tgacgtttct ccagcagcgt gcggtacgcc gggtgaactt tggtgtacag
    28921 cttggagatt tccgcctctt taaaggtcag ttcgttcaac tgcgcgtcga tgttcaccat
    28981 cgaatcgagc accgctttcg cttccagcgg cagatcaaca gaatctttat cctgacggaa
    29041 ggcattcagt ttgttttcgg caacatcgag gcggctacgt acttccggta actgttgcgc
    29101 gaggaaagcg aggcttttcg acgcttccgc cgatttgcgc tcaatatttt gttcctgata
    29161 gttacgggcg atgctgttaa gaatgtcgcg gatctgttcg cgatcttcac cggtataagt
    29221 caggctcagt acgcctgcgt ctttgccgtt ctccgttacc gtcaggctgt tttgcagttg
    29281 gttgatcatc cccaacgtgg agtatttggt gacggtaaac tcactgcccg ggcgggcgtg
    29341 aatggcttca accatcagcg tgacgccttc ttttttcagc atctggcccg cttgcccacg
    29401 ggcgctaaag ccgccatcac tgctcagggt gtagttttta ttgtccagca cattaagcgt
    29461 aaacacctgg cccgccatct ctttcgggcg gttaaaggta gtcactttca ccgtctcgtt
    29521 ctggcgtccc atcagtcgct cccagcccgc cccgaaaatc gggaacgtgt ttttgctcac
    29581 tgcaatatcg aggtcgagat cgtccaccgt tttaccaagc accagacgcg aacgaatcaa
    29641 ctggatctcg gcgtccgatg caggcggttt gttggctaat gccgaaccaa tgtcctgcac
    29701 taacgaattg ccgctgtttt gctcgatttg taccagtgcg tcggcactat aaatcggcgt
    29761 tgcgaagaag gtgtaaacca cggcacagag ggcaaataca gcggtgatgc caatcaccca
    29821 ccagcgcgct tcaatgacgg tgccgaccag gcgaccaata tcgatttcat cactgcccgt
    29881 taccggagcg gcatgttgtt ttactttttc tgtcattctt atacctgctc tgcgttcaat
    29941 gcctgcgccc actggcgggc agaccgttca agtaatgtgt acaccgctgc aaacgtttcc
    30001 cggcttttgc gatacggatc ggggatttca cattcgttat cccagtgacc aaacagcatc
    30061 actttgccgc gcatctcagg tgccatctcg cataagcgtt cgatatggcg cttttccatg
    30121 gtcaaaatca ggtcatagtt gcgacacagg cggcggctga tttgtcgtgc acagtggcct
    30181 tccagagaca gttgatgttc tgcggcgacg ctgatagcgg taggatcagc gcccttaccg
    30241 accagcgcgc cgagtccagc ggactccact ttcagttccg ggtgataacg ttgcagtaag
    30301 cgttccgccg tcggggaacg gcaaatattg ccgacacaga caactaatat gttgttaaac
    30361 atgacgatta ccagttatga atgtcgctgg ctgtatccgt catgtaacgg acaccgctaa
    30421 tagttggcaa caactgattg atcagacggt tccagcggga aaccggggcg gtggtgacat
    30481 acaccacgtc ataaggttgc aggcggaatt ctgtcgccat caccagcgac gtggcatcgg
    30541 acatatccag ctggtagata ttggcaatct tgccattacg cccgccctcg cctttcagcg
    30601 gacgaatgac aaagatgccg ctggcgttgg aggtggtcat gtcgatacct tcagcattac
    30661 ccagggcttc agtcagggtc atgccgctaa agtccatttt cagggtgctc tgtttcttca
    30721 cttcacccat cacaaatact ttcagatcat cattacgcgg cacgtagaga atatcgccgg
    30781 gataaagcag tcggttctga ttgaggtcgc cgttttgcat cagcgcctgc aaagaaatgc
    30841 gttcttcacg accattgtgt gttagcacta cgttgcgcca gtcagcggtg tcggtcaggc
    30901 cacctgcggc gttgatggcg tcgagaatag tcagtggcac gttggtgatc gcctgttgac
    30961 cggatttatt cacctgaccg gagatatagg ccttttgtga gcggaaggcg gcgatattaa
    31021 cgtccacctg cgggtcagcg atgtacgtcg ctaagcgccc ggtaatatca ctgcggattt
    31081 cagcgagcgt tttcccgact acgtgaacct tgccgatata cgggtaaaac atagtgccgt
    31141 caggctgtac ccagttgccg gtgtcgctgg agctgcggta ctgaccggct ggcgtggtga
    31201 gttccgggtg atcccagacg gtgacattca gaacgtcgcc cggcccgacg cgatactggt
    31261 aattcgcgat ctcgctttcc agcgtcatat tggggcgcgc tacattcggg cgtgggcgta
    31321 attggtcaat caggcgcggg gtcagcggat aaacattcac cattttgtcg agatcgaaat
    31381 cagcgtcctg ctgtttgatg acgtctttgc ccatcgtcga catattgctg cccggaagta
    31441 ctgtgcaacc gcttatcaag gttactgaca ccaataatgg catcaatttc attttggatt
    31501 tcatcattgt ttatttatca ctttggcaga gtaattatcc tgtgcactat taatagcaat
    31561 atcgccatgc acatttacct tgcagttaat tgaataaaaa tttaactggc atcagtccta
    31621 aaaaaattga tttcatccgc aggctattga cagaataatt cagactggtc tttcaggcat
    31681 ccagacacgc taccgcccct ggctttttag ccaccaacac actgatttag cttaattttt
    31741 caaaccctct ctgcatacaa tcgttgatga gaaagggtta ttgcggaaat taacttccga
    31801 atataaggtg acattatggt aattgaatat tggctctcca ataa
//
LOCUS       DAZOZD010000023        30951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOZD010000023 DAZOZD010000000
VERSION     DAZOZD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.023"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUPB_000859"
     CDS             267..1925
                     /locus_tag="ACHUPB_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0757752.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUPB_000860"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUPB_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0757753.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUPB_000861"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUPB_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0757754.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUPB_000862"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUPB_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0757755.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUPB_000863"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUPB_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0757756.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUPB_000864"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUPB_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0757757.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUPB_000865"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUPB_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0757758.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUPB_000866"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUPB_000866"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0757759.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUPB_000867"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUPB_000867"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUPB_000868"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUPB_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0757760.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUPB_000869"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUPB_000869"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0757761.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUPB_000870"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUPB_000870"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0757762.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUPB_000871"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUPB_000871"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0757763.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUPB_000872"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUPB_000872"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0757764.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUPB_000873"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUPB_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0757765.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUPB_000874"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUPB_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0757766.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUPB_000875"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUPB_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0757767.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUPB_000876"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUPB_000876"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0757768.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHUPB_000877"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHUPB_000877"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0757769.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUPB_000878"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUPB_000878"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR0757770.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUPB_000879"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUPB_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0757771.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUPB_000880"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUPB_000880"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR0757772.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUPB_000881"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUPB_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0757773.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHUPB_000882"
     CDS             29803..30951
                     /locus_tag="ACHUPB_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0757774.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7623 a         7623 c         8348 g         7357 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcac
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtacgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcttc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggct tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta a
//
LOCUS       DAZOZD010000024        30782 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOZD010000024 DAZOZD010000000
VERSION     DAZOZD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.024"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHUPB_000883"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHUPB_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0757775.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHUPB_000884"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHUPB_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0757776.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHUPB_000885"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHUPB_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0757777.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUPB_000886"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUPB_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0757778.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUPB_000887"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUPB_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0757779.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUPB_000888"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUPB_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0757780.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUPB_000889"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUPB_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0757781.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUPB_000890"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUPB_000890"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0757782.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUPB_000891"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUPB_000891"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0757783.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUPB_000892"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUPB_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0757784.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUPB_000893"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUPB_000893"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0757785.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUPB_000894"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUPB_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0757786.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUPB_000895"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUPB_000895"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0757787.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUPB_000896"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUPB_000896"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0757788.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUPB_000897"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUPB_000897"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0757789.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHUPB_000898"
     CDS             complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHUPB_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR0757790.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUPB_000899"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUPB_000899"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0757791.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUPB_000900"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUPB_000900"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0757792.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUPB_000901"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUPB_000901"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0757793.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHUPB_000902"
     CDS             21688..22278
                     /locus_tag="ACHUPB_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0757794.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUPB_000903"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUPB_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0757795.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHUPB_000904"
     CDS             22750..23445
                     /locus_tag="ACHUPB_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0757796.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUPB_000905"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUPB_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0757797.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUPB_000906"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUPB_000906"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0757798.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUPB_000907"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUPB_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0757799.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUPB_000908"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUPB_000908"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0757800.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUPB_000909"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUPB_000909"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0757801.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
     gene            30310..>30782
                     /locus_tag="ACHUPB_000910"
     rRNA            30310..>30782
                     /locus_tag="ACHUPB_000910"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7234 a         7995 c         8488 g         7065 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaaatcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaagcgcgtc gcaactgctc tttaacaatt
    30181 tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca agacgaaaaa
    30241 tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt ctttgagcat
    30301 caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg gcaggcctaa
    30361 cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga gtggcggacg
    30421 ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta
    30481 ataccgcata atgtcgcaag actaaagagg gggaccttcg ggcctcttgc catcggatgt
    30541 gcccagatgg gattagctag taggtggggt aacggctcac ctaggcgacg atccctagct
    30601 ggtctgagag gatgaccagc cacactggaa ctgagacacg gtccagactc ctacgggagg
    30661 cagcagtggg gaatattgca caatgggcgc aagcctgatg cagccatgcc gcgtgtatga
    30721 agaaggcctt cgggttgtaa agtactttca gcggggagga agggagtaaa gttaatacct
    30781 tt
//
LOCUS       DAZOZD010000025        30663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOZD010000025 DAZOZD010000000
VERSION     DAZOZD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.025"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            103..507
                     /locus_tag="ACHUPB_000911"
     CDS             103..507
                     /locus_tag="ACHUPB_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0757802.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            533..1246
                     /gene="msyB"
                     /locus_tag="ACHUPB_000912"
     CDS             533..1246
                     /gene="msyB"
                     /locus_tag="ACHUPB_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0757803.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            1395..1961
                     /gene="satP"
                     /locus_tag="ACHUPB_000913"
     CDS             1395..1961
                     /gene="satP"
                     /locus_tag="ACHUPB_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0757804.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1996..2583)
                     /gene="mog"
                     /locus_tag="ACHUPB_000914"
     CDS             complement(1996..2583)
                     /gene="mog"
                     /locus_tag="ACHUPB_000914"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0757805.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2698..3651)
                     /gene="tal"
                     /locus_tag="ACHUPB_000915"
     CDS             complement(2698..3651)
                     /gene="tal"
                     /locus_tag="ACHUPB_000915"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0757806.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3930..5359
                     /locus_tag="ACHUPB_000916"
                     /pseudo
     CDS             3930..5359
                     /locus_tag="ACHUPB_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            5429..6205
                     /gene="yaaA"
                     /locus_tag="ACHUPB_000917"
     CDS             5429..6205
                     /gene="yaaA"
                     /locus_tag="ACHUPB_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0757807.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6358..6654)
                     /locus_tag="ACHUPB_000918"
     CDS             complement(6358..6654)
                     /locus_tag="ACHUPB_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0757808.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(6868..8154)
                     /gene="thrC"
                     /locus_tag="ACHUPB_000919"
     CDS             complement(6868..8154)
                     /gene="thrC"
                     /locus_tag="ACHUPB_000919"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0757809.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(8155..9087)
                     /gene="thrB"
                     /locus_tag="ACHUPB_000920"
     CDS             complement(8155..9087)
                     /gene="thrB"
                     /locus_tag="ACHUPB_000920"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0757810.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(9089..11551)
                     /gene="thrA"
                     /locus_tag="ACHUPB_000921"
     CDS             complement(9089..11551)
                     /gene="thrA"
                     /locus_tag="ACHUPB_000921"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0757811.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11578..11697)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11633..11698)
                     /gene="thrL"
                     /locus_tag="ACHUPB_000922"
     CDS             complement(11633..11698)
                     /gene="thrL"
                     /locus_tag="ACHUPB_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0757812.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11912..12598)
                     /locus_tag="ACHUPB_000923"
     CDS             complement(11912..12598)
                     /locus_tag="ACHUPB_000923"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0757813.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(12998..13138)
                     /gene="yjjY"
                     /locus_tag="ACHUPB_000924"
     CDS             complement(12998..13138)
                     /gene="yjjY"
                     /locus_tag="ACHUPB_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0757814.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            13234..13950
                     /gene="arcA"
                     /locus_tag="ACHUPB_000925"
     CDS             13234..13950
                     /gene="arcA"
                     /locus_tag="ACHUPB_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0757815.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(14010..15362)
                     /gene="creD"
                     /locus_tag="ACHUPB_000926"
     CDS             complement(14010..15362)
                     /gene="creD"
                     /locus_tag="ACHUPB_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0757816.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(15420..16844)
                     /gene="creC"
                     /locus_tag="ACHUPB_000927"
     CDS             complement(15420..16844)
                     /gene="creC"
                     /locus_tag="ACHUPB_000927"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0757817.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(16844..17533)
                     /gene="creB"
                     /locus_tag="ACHUPB_000928"
     CDS             complement(16844..17533)
                     /gene="creB"
                     /locus_tag="ACHUPB_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0757818.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17546..18019)
                     /gene="creA"
                     /locus_tag="ACHUPB_000929"
     CDS             complement(17546..18019)
                     /gene="creA"
                     /locus_tag="ACHUPB_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0757819.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            18230..19099
                     /gene="robA"
                     /locus_tag="ACHUPB_000930"
     CDS             18230..19099
                     /gene="robA"
                     /locus_tag="ACHUPB_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0757820.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(19096..19743)
                     /gene="gpmB"
                     /locus_tag="ACHUPB_000931"
     CDS             complement(19096..19743)
                     /gene="gpmB"
                     /locus_tag="ACHUPB_000931"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0757821.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19795..20316
                     /gene="yjjX"
                     /locus_tag="ACHUPB_000932"
     CDS             19795..20316
                     /gene="yjjX"
                     /locus_tag="ACHUPB_000932"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR0757822.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(20401..20727)
                     /gene="trpR"
                     /locus_tag="ACHUPB_000933"
     CDS             complement(20401..20727)
                     /gene="trpR"
                     /locus_tag="ACHUPB_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0757823.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(20817..22754)
                     /gene="sltY"
                     /locus_tag="ACHUPB_000934"
     CDS             complement(20817..22754)
                     /gene="sltY"
                     /locus_tag="ACHUPB_000934"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0757824.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22965..24632
                     /gene="ettA"
                     /locus_tag="ACHUPB_000935"
     CDS             22965..24632
                     /gene="ettA"
                     /locus_tag="ACHUPB_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0757825.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(24793..24900)
                     /gene="nadS"
                     /locus_tag="ACHUPB_000936"
                     /pseudo
     CDS             complement(24793..24900)
                     /gene="nadS"
                     /locus_tag="ACHUPB_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(24939..26171)
                     /gene="nadR"
                     /locus_tag="ACHUPB_000937"
     CDS             complement(24939..26171)
                     /gene="nadR"
                     /locus_tag="ACHUPB_000937"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0757826.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(26192..27574)
                     /gene="radA"
                     /locus_tag="ACHUPB_000938"
     CDS             complement(26192..27574)
                     /gene="radA"
                     /locus_tag="ACHUPB_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0757827.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(27623..28591)
                     /gene="serB"
                     /locus_tag="ACHUPB_000939"
     CDS             complement(27623..28591)
                     /gene="serB"
                     /locus_tag="ACHUPB_000939"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0757828.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            28697..29341
                     /locus_tag="ACHUPB_000940"
     CDS             28697..29341
                     /locus_tag="ACHUPB_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0757829.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            29369..30385
                     /gene="lplA"
                     /locus_tag="ACHUPB_000941"
     CDS             29369..30385
                     /gene="lplA"
                     /locus_tag="ACHUPB_000941"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR0757830.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            30417..30663
                     /locus_tag="ACHUPB_000942"
                     /pseudo
     CDS             30417..30663
                     /locus_tag="ACHUPB_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         7298 a         8351 c         7651 g         7363 t
ORIGIN      
        1 aattgtaata aaaagaaaag attacgtacc tgaatcttct ctttatcagc agtaaactag
       61 tgggtattca tccctctacc tcttcccact aagagaatcc ttatgaaatc cgtttttacg
      121 atttccgcca gcctggcgat tagcctgatg ctgtgctgca cggcgcaggc aaacgaccat
      181 aaactcctcg gcgtcattgc aatgccgcgt aacgaaacca acgatctggc gctgaaactt
      241 cctgtttgtc gcattgtgaa acgcatacaa ctctctgccg accatggcga tttacagtta
      301 agcggtgcat cggtttattt caaagccgcc cgtagcgcca gtcagagtct gaatattcct
      361 tcagaaataa aagaagggca aaccactgac tggatcaaca ttaacagtga taacgacaat
      421 aaacgctgcg tctcaaaaat caccttttcc ggtcatacgg tgaactcatc ggatatggcc
      481 acgctgaaaa ttatcggcga cgactaacgc cagaatatag aaaccacaaa aaatgaatgt
      541 taattacctg aatgattcag atctggattt tcttcagcat tgtagtgagg aacagttggc
      601 aaatttcgcc cgattgctca cccataatga aaaaggcaaa actcgcctct ccagcgtact
      661 gatgcgcaac gaactgttta aatcgatgga agggcatccc gagcaacatc gccgcaactg
      721 gcagctgatt gctggagaat tacagcattt tggtggcgat agtatcgcca acaaactgcg
      781 cggacacggt aaattgtatc gggccatttt gctcgatgtt tcaaaacgat tgaagctgaa
      841 agccgacaaa gagatgtcta cgtttgagat tgagcaacag ttactggaac aatttctgcg
      901 taacacctgg aagaatatgg acgaggaaca taagcaggag tttctgcacg cggtcgatgc
      961 cagggtgaat gagctggaag agctgctgcc gctgctgatg aaagacaaat tattggcaaa
     1021 aggcgtgtcg catttgcttt ccagccaatt gacccgcatt ttacgcaccc acgcagcaat
     1081 gagcgtgctt gggcatggtt tgctgcgcgg cgcgggtctg ggaggccctg tgggcgcagc
     1141 attaaatggg gttaaagcgg tcagcggcag tgcctatcgc gtgacaattc cagccgtaat
     1201 acaaatcgcc tgcctgcgcc ggatggttag cgccactcag gtctgacacc gtaaaccggg
     1261 gaaaattttc attttcccct caaaagatcg tagacactgc cccactggct gattattatg
     1321 ccgcgccctg aaaacactac tgttattcag ggaaattatt tcaccattca ttcgatgatg
     1381 atttttgagg aattatgggc aacactaagt tggctaatcc ggcaccgctg ggcctgatgg
     1441 gcttcggcat gaccaccatt ctgcttaacc tgcacaacgt gggttatttc gctctggacg
     1501 gtattattct tgccatgggc attttctacg gcggcatcgc gcaaattttt gccggtctgc
     1561 tggagtacaa aaaaggcaac actttcggtt taaccgcatt cacctcttac ggttctttct
     1621 ggttgacgct ggttgcgatt ctgctgatgc cgaaactggg tctgaccgat gcgccaaatg
     1681 cacagttcct tggtgtctac ctgggtctgt ggggcgtatt tacgctgttt atgttcttcg
     1741 gcacgctgaa aggcgcacgc gttctgcaat tcgttttctt tagcctgact gtgctgtttg
     1801 ccctgctggc gatcggtaac attgccggta acgccgcaat catccacttt gccggctgga
     1861 ttggtctgat ctgtggtgcc agtgcaatct atctggcgat gggtgaagta ctgaacgagc
     1921 agtttggtcg caccgttctg ccgattggtg aatcccacta attgttttga gatccccccg
     1981 ctcgggggga tttttttatt cgctaacgtc gcgtcttgca ctcttcggtc tgaatgcagc
     2041 aaccacttct ggtgccgttt caacgtatgg cccttccagc aactgaatgc agtacggtac
     2101 gctggcaaaa ataccgtgta ccacaacgtt acccgcagcg tccttcacac cttccagcgt
     2161 ctctttaata gacttcggct gaccgggtaa gttaaggatc agcgcctgtt tgcgaatcac
     2221 accaacctga cgcgaaagga tcgcagttgg tacaaaatgc aggcttatct ggcgcatctg
     2281 ttcaccaaag ccaggcatct cgcggtccgc tactgccagc gtcgcatcgg gcgttacgtc
     2341 acgacgcgcc gggccagttc cgcccgtggt gagcaccaga tggcaactca tttcatccac
     2401 tagctcacac aacgtttgct cgatgatcgc ctgctcatcg gggattaagc gggtttccag
     2461 ttcaaacggc gtggttagcg ccgatgtcag ccattcttcc agcgcaggga tgcctttatc
     2521 ctgataaacg ccgctggatg cgcgatcaga gatggaaact aagccaatac gtaaagtatt
     2581 catcaagatt tttccgttta aacagtctcg ttaaacagaa tgatacactg cgaagggagt
     2641 gacagacagg cttcagaaga ggtagcgtga ccgacttccc ggtcacgcta agaatgatta
     2701 cagcagatcg ccgatcattt tttccagttt ttcctggtca atagcaaact tacggatacc
     2761 ttccgccagt ttatctactg ccattggatc ctggttgtgc tgccacagga actcggactc
     2821 agtgatacgc gccggacgcg ctttcacttc gccggtgtaa gacagtttac gttcgatagc
     2881 cccttcgctc tcagccagct ctttcagcag tgccggtgcg atggtaagac ggtcgcagcc
     2941 tgccagttcc agaatttcgc cgatgttacg gaagcttgcg cccataacca cggtttcata
     3001 accgtgctct ttgtagtact ggtagatttc agatacagaa accacgcccg gatcttctgc
     3061 cggagcgtac tctttcttat cagtattcgc tttgtaccag tcaagaatac ggccaacaaa
     3121 tggcgagatc aggaacacgc ctgcttccgc acaagcacga gcctgagcga aggagaacag
     3181 cagggtcagg ttacagttga tgccttcttt ttccagctgt tctgcagcac ggataccctg
     3241 ccaagtagaa gccagtttga tcagaatacg atcgttgcta ataccagcat cgttgtagag
     3301 tttgatcagg cgttttgctt tcgcaattga cgcttcggtg tcataggaaa gacgcgcatc
     3361 aacttcagtt gagatacggc ccggaaccag tttcaggatt tccagaccaa tatttactgc
     3421 cagtttgtcg gtcgcgtcca cgatctgctg cgcgcgatcg ttgctctgct gtttcgccca
     3481 ggcgacagca tcatcaatca acttacggta ttccggaatc tgcgctgcgt taagaatgag
     3541 agaagggttg gttgtggcat cctgcggttg atacagcttc attgccgcga tgtccccagt
     3601 gtcggccact acggtggtgt actgacgaag ggaggtcaat ttgtccgtca tgatagtatt
     3661 tctctttaaa cagcttgtta gggggatgta accggtctgc cctgatgata tcacgacgca
     3721 acgtcagcgc aaccgccgac aagacggtta tcgccaggcg agactgtttc ggatttctga
     3781 cactgcgttg atatcgcccg ccatttttat acaaaacctc atgtatgcta cgcagaagtt
     3841 atcaagtacc tcgtagcgta tatacttctt aaacaattgg taacgtttac acaggaaagt
     3901 catcgcgacc ggcaataaga gggatatgca tgccagattt tttctccttt attaacagcg
     3961 tcctttgggg atcggtaatg atttacctgc tcttcggcgc aggttgttgg ttcacttttc
     4021 gcaccggatt tgtgcagttt cgctacatcc gccagtttgg caaaagtctt aaaaatagca
     4081 ttcatccaca gccaggcggt ttaacctcat ttcagtcatt gtgtaccagt cttgcggcgc
     4141 gcgtgggtag cggcaacctg gccggcgttg cgctggctat taccgccggt ggacctggag
     4201 ccgtcttttg gatgtgggtt gccgcgttta tcggcatggc gacctcgttt gccgaatgtt
     4261 cccttgcaca actttataaa gaacgtgacg tcaatggaca gtttcgtgga ggaccggcat
     4321 ggtatatggc gcgcgggctg gggatgcgtt ggatgggcgt tctgttcgcc gtctttttgc
     4381 tcatcgccta cggcataatt ttcagcggag ttcaggcgaa cgccgttgcc cgagccctga
     4441 gttttctttt gattttcccc cgctggtgac aggcattatt ctcgctgtct ttgctctgct
     4501 ggcaatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat
     4561 ggcgataatc tgggtgctga ccagcatggt gatttgcgta ataaatatcg ggcaacttcc
     4621 ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg caggcggcgc
     4681 ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa
     4741 tgaggcggga atgggttcga cgccaaacgc ggcagcggca gcggcgtcct ggcctccgca
     4801 tccggcagcg caagggattg tccagatgat tggcattttt atcgacaccc tggtcatctg
     4861 tacggcaagc gccatgctga tattactggc gggtaacgac acaacctaca tgccgctgga
     4921 aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctggg gtgctgagtt
     4981 tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc
     5041 cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtttgcggat
     5101 ctgcaccttc gcaacggtca tcggcggcac cttgctaagt cttccgctga tgtggcaact
     5161 ggcagatatc ataatggcct gcatggctat taccaatttg accgccattt tactgctctc
     5221 gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgccc
     5281 ggtgtttgat ccgttgcgtt atccggatat cggtcgccag ctttctccgg acgcgtggga
     5341 tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt
     5401 cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct
     5461 tgattaccaa agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaattc
     5521 ccagcacttg atccatgaag cgcggaaact gacgcctccg cagattagca cgctgatgcg
     5581 catcagcgac aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccagattt
     5641 cacgccggag aatgcccgcc aggctatttt ggcgtttaaa ggtgatgtct acaccggctt
     5701 gcaggccgaa actttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct
     5761 ttccggcttg tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat
     5821 ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat
     5881 caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga ttaatctggc
     5941 gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc
     6001 ggtgttcctc gatgagaaga acggcaaatt taagataatc agcttctacg ctaagaaagc
     6061 acgcggtctg atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac
     6121 tggttttaat agcgaaggtt acttctttga tgaagcttcc tccagcaatg gcgaactggt
     6181 gtttaaacgc tacgagcagc gttaagtttg cagtcaatgc cggatgcggc gtgaacgcct
     6241 tatccggcct acaaaagcat gcagattcaa tatattgcag aattcacgta ggcctgataa
     6301 gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat ttgtcattta
     6361 gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgaggagctt tcttatggtg
     6421 gcgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt cataatgttg
     6481 tttccaccag ccgtggtcgc gccagtgacc tccatcccag taatagccac gattatcacg
     6541 atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct gtgctgccat
     6601 gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt tcacaggtta
     6661 ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg agaacgacgg
     6721 ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat ttctcctttt
     6781 tccctgtcgt ttttctccat aatttcttca taaaaaagcc gggctgcata aaagcaaacc
     6841 cggcctgatt gagataatga atagatttta ctgatgattc atcatcaatt tacgcaacgc
     6901 agcaaaatcg gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt ctgccagctc
     6961 ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa atttcgccgg
     7021 atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac acagcgcacg
     7081 atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact cacgcattgt
     7141 ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt gccagatttt
     7201 gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt ccatcgcgtt
     7261 ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga aacgtggcac
     7321 ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga gtgacttcgc
     7381 cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa ccagctggtt
     7441 gcgcgcttcc tgcggcagct gcgcaactgc ttcaaagtag tagcaaatct gcgccagcaa
     7501 acggctgatg ttaatcgagt tagccgagtt taaccccagc gccactttca gttcttcatc
     7561 atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga tggcaacagt
     7621 ttcgatattg ccgcccaacg tacagaacag tttttcttgc agtggactga ttttgcctcg
     7681 tggatagagg ataaccactt tcacattcgg caaaccgtag aaagcatgag ccactgccgc
     7741 tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg caatatgggt
     7801 cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg gcccgtggaa
     7861 caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga aggcaaacgc
     7921 cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa acgccgagag
     7981 gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt cagtcaggct
     8041 gaattccggc aggtcatgcg gaaaaaacag cccctgattt ttgcccaacc cctgggttac
     8101 ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt tcatttagtt
     8161 ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac cttcctgatt
     8221 ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg gcttgtcaca
     8281 tagagcgaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc cgatttccgc
     8341 gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg gttcagcgat
     8401 aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg cgtgaatgaa
     8461 gccagccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg ccggtaaaat
     8521 agcccgggct tctgccgtcg agactttaat tcccggatac gccagcaccc acagccactc
     8581 atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca actgcatacc
     8641 accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac gtccttccag
     8701 ctcgcccatc aaagccagca aacgggtgtc attaagtggc ttgccgcagt gttcattcat
     8761 cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgagc cgatcggcat
     8821 attcttttcc agagtcatcg ccactggaat ttgcttgccc agctccaggc aaaaacgctc
     8881 ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg caaagcgtcc
     8941 gaggttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc cgagcaatgc
     9001 accatcaacg ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca tattggcact
     9061 ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag agggtacgta
     9121 gcagatcggc aaagacacca gcagcggtaa cgtcattgcc cgcaccatat ccgcgcagca
     9181 ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg ccatttttca
     9241 ctttgaacag cggatcattc ccatccactt cggcaatctt cacgcggcag acaccatctt
     9301 catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc gccacgcgcg
     9361 cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca tcaccctcgg
     9421 cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagttcc agttcacgtc
     9481 ccgtttcacg agcgagaatc aatagtttac gcgccacatc cataccagaa agatcatctc
     9541 gcgggtccgg ttcggtataa cccatttccc gcgccagcgt ggtcgcctcg gagaaactca
     9601 tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg ccggagaact
     9661 tcaccaattc atcacctgca ttgagcagat tttgcaggtt ctcaataacc ggtaatccag
     9721 ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca taacgcaact
     9781 gatgatagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca acgtggaaac
     9841 cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg cagtcaacaa
     9901 tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg agattaaacg
     9961 gctctttggc ttgtgcaagt tcttcctgcc agttttccag attaaggcca tgcacactag
    10021 tgagcagagc cttcgagttg gcaacgccgc agacacgtaa gtcgatatgt ttattcttca
    10081 gccagctttg ctgacgcttc agttgctcca gcagcgcacc gccaacgcca ccgacgccaa
    10141 tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta acgcgcacgc
    10201 cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa gatccctgag
    10261 caatggcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc gccgagatcc
    10321 cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt tccatcaccg
    10381 ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc attgcccgtt
    10441 cagcccgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg gaagatgatt
    10501 gcgtaatcag caccacggaa atacgggcgc gtgacatcgc ggcaaagacg cgcgccgcca
    10561 tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccatg ttattcagat
    10621 tggaaatgcc cttgaccggt aattcgcctt catcacgact ggcaccaatg agcgtacctg
    10681 gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg gcgatggggg
    10741 taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc gcttcctggt
    10801 aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag gtatagaccc
    10861 cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc agcaccgccg
    10921 cggagtagtc ggaaccgttg cgtccaagta ccaccagttc gcctttttca ttaccggcgg
    10981 tgaagcctgc catcagcacc atgtgatcag ccggaatacg acttgccgca atacggcggg
    11041 tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagt agtttttcga
    11101 ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc ataatggcga
    11161 tcgacatttt ctcgccacgg caaatcagcg cagcgttaat gctatccggg cactgcccta
    11221 acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg acgaaagttt
    11281 tcaattgcgc cagcgggaat cccggctggg cggcggcgag tcccgtcaaa agttcggcaa
    11341 aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg gttttttcaa
    11401 tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg gccacctgcc
    11461 cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct gcatttgcca
    11521 ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct ttggtcgaaa
    11581 aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg cgtcagcccg
    11641 caccgttacc tgtggtaatg gtgatggtgg tggtaatggt ggtgctaatg cgtttcatgg
    11701 atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat gcctatattg gttaaagtat
    11761 ttagtgacct aagtcaataa aattttaatt tactcatggc aggtaaccag ttcagaagct
    11821 gctatcagac actctttttt taatccacac agagacatat tgcccgttgc agtcagaatg
    11881 aaaagctgaa aaatacttac taaggcgttt tttatttggt gatatttttt tcaatatcat
    11941 gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa aagccccagg cgttgctgta
    12001 accagtcgac cagttttatg tcatctgcca ctgccagagt cgtcagcaat gccatgactc
    12061 gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt gtcacttttc gccggttgtt
    12121 gtattaatgt tgctaattga tagcaataga ccatcaccgc ctgccccaga ttgagcgaag
    12181 gataatccgc caccatcggc acaccagtaa gaacgtcagc caacgctaac tcttcgttag
    12241 tcagcccgga atcttcgcga ccaaacacca gcgcggcatg gctcatccat gaagattttt
    12301 cctctaacag cggcaccagt tctactggcg tggcgtagta atgatatttc gcccgactgc
    12361 gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga ttcagccagt gttgggaaaa
    12421 ctttaatatt atcaataaca tcaccagatc catgtgcgac ccagcgggtg gctggctcca
    12481 ggtgtgcctg actatcgaca atccgcagtt cgctaaaacc catcgttttc attgcccgcg
    12541 ccgctgcccc aatattttct gctctggcgg gtgcgaccag gataatcgtt atacgcatat
    12601 tgccactctt cttgaccaaa taaccgcgaa cagggtgatc actgtcaact tattacgcgg
    12661 tgcgaattta caaattctta acgtaagtcg cagaaaaagc cctttactta gcttaaaaaa
    12721 ggctaaacta tttcctgact gtactaacgg tttaattgtt aaaaaatgct acatatcctt
    12781 ctgtttactt aggataattt tataaaaaat aaatctcgac aattggattc accacgttta
    12841 ttagttgtat gatgcaacta gttggattat taaaataatg tgacgaaagc tagcatttag
    12901 atacgatgat ttcatcaaac tgttaacgtg ctacaattga acttgatata tgtcaacgaa
    12961 gcgtagtttt attgggtgtc cggcccctct tagcctgtta tgttgctgtt aaaatggtta
    13021 ggatgacagc cgtttttgac actgtcgggt cctgagggaa agtacccacg accaagctaa
    13081 tgatgttgtt gacgttgatg gaaagtgcat caagaacgca attacgtact ttagtcatgt
    13141 tacgccgatc atgttaattt gcagcatgca tcaggcaggt cagggacttt tgtacttcct
    13201 gtttcgattt agttggcaat ttaggtagca aacatgcaga ccccgcacat tcttatcgtt
    13261 gaagacgagt tggtaacacg caacacgttg aaaagtattt tcgaagcgga aggctatgat
    13321 gttttcgaag cgacagatgg cgcggaaatg catcagatcc tctctgaata tgacatcaac
    13381 ctggtgatca tggatatcaa tctgccgggt aagaacggtc ttctgttagc gcgtgaactg
    13441 cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc gtgacaacga agtcgataaa
    13501 attctcggcc tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa
    13561 ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa
    13621 gaacgtcgta gcgttgaaag ctacaagttc aatggttggg aactggacat caacagccgt
    13681 tcgttgatcg gccctgatgg cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg
    13741 cttcacttct gtgaaaaccc aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg
    13801 accggccgtg agctgaaacc acacgaccgt actgtagacg tgacgatccg ccgtattcgt
    13861 aaacatttcg aatctacgcc ggatacgccg gaaatcatcg ccaccatcca cggtgaaggt
    13921 tatcgcttct gtggtgatct ggaagattaa tcggctttac caccgtcaaa aaaaacggcg
    13981 ctttttagcg ccgtttttat ttttcaacct tatttccaga tacgtaattc atcgtccgtt
    14041 gtaacttctt tactggcttt cattttcggc agtgaaaacg cataccagtc gatattacga
    14101 gtcacaaaca tcatgccggc cagcgccacc accagcacac tggttcccaa caacagcgcg
    14161 ctatcgacag agttgagcag tccccacatc actccatcca gcaacaacag cgcgagggta
    14221 aacaacatgc tattgcgcca gcctttcaat accgcctgca aataaatacc gttcattaac
    14281 gccccaatca gactggcgat tatccatgcc acggtaaaac cgatatgttc agaaagcgcc
    14341 agcaagagca aataaaacat caccaatgaa agccccacca gcaaatattg cattgggtgt
    14401 aaacgttgcg cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag tgcaatcagc
    14461 agaatggcgt acttagtcgc ccggtccgtt aattggtatt gatcggctgg cgtcgtcact
    14521 gcgacgctaa acgcgaggaa gttttcccag ccggtatcat tgcctgaagc aaaacgctca
    14581 ccgagattat tagcaaacca gctgctttgc cattgcgcct gaaaacctga ctcgctaact
    14641 tcccgtttgg ctggcaaaaa atcacctaaa aaactgggat gcggccagtt gctggttaag
    14701 gtcatttcgc tattacgccc ggcaggcacc acagaaagat cgccggtacc gcttaaattc
    14761 agggccatat tcagcttcag gttctgcttc cgccagtccc cttcgggtaa agggatatgc
    14821 acgccctgcc cgccttgctc taacccggtg ccgggttcaa tggtcagcgc cgttccgtta
    14881 acttcaggcg ctttcaccac accaatacca cgcgcatccc cgacgctaat cacaataaat
    14941 ggcttgccaa aggtgatatt tggcgcgtcg agttcgctaa gacgcgaaac atcgaaatcg
    15001 gcttttagcg ttaaatcact gtgccagacc tgaccggtat aaatccctat cttgcgttct
    15061 tccacgttct gattgccatc aaccatcaat gactcaggta accaaaaatg gataaaactt
    15121 cgtttccgct ccacggtttt atcctcttcc tgtaccgtat aaagctcggt cacaggaata
    15181 gcgataagcg gcccaacaag tttttgcggc ccgctggtac tttggcgaat cgcatcttcc
    15241 acatcgctac ggtaatcggc acgttcgaca atcacctgcc gaatcagcat tatcggaatc
    15301 aacaacagca atactgcacc aaacagggtg gtcattttcc agaacaggga ggatttcaac
    15361 atagtcttct cctttgcaat ggcagcagga tacgaaggct atgtgggaag aatttgaagc
    15421 tatgtgaagt gacggtgaag tcgaagcgag gccagcacgc caccttcctg cacgttgcgc
    15481 agcgtgactt cgccgttaaa caaacgggcg acctcactga cgaacgccaa ccccagaccg
    15541 ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc
    15601 gcgtagtcag gaataccact accggtatcc agcaccttaa gcgtgacgtg ttcctgatcc
    15661 acttcggcgc ttagcgttat acgaccgctc tcgggggtaa aatcgatggc gttatccagt
    15721 aaattcccca gcgcctgatc cagtaacgcc ggttcagcag caacattaac ctcggtgggc
    15781 gtaacatgta gagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg
    15841 cggaataatg ccgccacatc aacagcagtc aaaacgactt cctgacgatt ctccagtctt
    15901 gcctggcgta gtaacgtttc caccagtgcc tgcattcgcg cattttgcgt cagaatgttg
    15961 tcagtaaaac gagccactac ttcaggcggc ggaccttcgc gtaaaatttc cgccgcgcca
    16021 cgaatcgccg ccagtgggct ttttagctca tgggttaacg cataaacata ctgctcaata
    16081 tagtttttcc cttccagctt cacgcgcata ctttccagcg cctgcgcgag tttacgcagc
    16141 tcgctactac cgagatcggg aagaggaacg ggcttattgt cagtgacgga atcagcatag
    16201 cgagtgagcc gggcaatgga gcggttgatc caccaaacca tgcctgcgcc aatcaccagt
    16261 gcgatcccca acaaaatggc gctggcccat aaaattcgcc gctcgctgcg tttaatgacc
    16321 ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg cgagccgtcc
    16381 ataatcggcg cggcaacata catcaccgaa ctttcgggat cggcaggatt ttgcaacgtg
    16441 ctacgcgcac catactgacc acgcaacgtt agccagacgt cattccagcg cgaataatcc
    16501 tgcccaacgg ctttatttgc cgaatcgaac aatactttgc cctgcgcatc ggtcatatag
    16561 acatgatatt cattgcgcac tttgttaatg ccaccgatat tggcgcgaaa cgggcgatgt
    16621 tgtagctgat taaacgcctg agccagttgc ccatgcgttg ggtccccaga gagcaaatcg
    16681 ggacgcgcca gctccgccag caacgttgcg gtatcgatta acgtcccctc cgttgctctt
    16741 cgcacgcccg gtttaacttc tttgacaaaa atagccagta cgaaccaggc cgccaccgcc
    16801 accagtaaaa aatagcccag cagcaaccgc atgccgatac gcattacagg cccctcaggc
    16861 tatatcccat gccgcgatga gtattaatcg gagaaagatc ggggttgatg gcgcgcagct
    16921 tggcacgcaa cgttttaatg tgggtatcga cggtgcgatc gtaggtgtcc tgcgcatctt
    16981 cccacacgct atccattagt tgctggcggg accagacgcg gcctggcgac ttgagtaacg
    17041 tcttcagcaa taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct
    17101 gcgccgcggg ttcattcaat tcaaaatgac caatacggat gacgggagac ggcgacgaga
    17161 acttcttcac ccgacgcagt aaggtgcgca ccctggcgca cacttcgcgg ggtgaaaacg
    17221 gtttagccac atagtcgtca gcgccaattt ccagcccaag caggcgatcg acctcttcac
    17281 ttcgggccgt caggaacagt acaggtaacg ccggatggag cgccagtaac tggcggcaca
    17341 attcaaagcc gctgatatca ggcaaaccaa catcgagaat catgacgtcg ggaacctgct
    17401 ggcgagcttt atccagcacc ggcaagcctc gctcaaagac ctcgacggca aaaccttcct
    17461 gctgcaacat gtagaccagc gtgtcggcta tcccttgctc atcttccact aaccagaccg
    17521 tttcccgttg cataaatcgc ctctgttatt gccgccacgg catgacagga accgcgctaa
    17581 tcgcgttttt cggcgaacct tctacaactt tgtcggagta agccagataa gcgagcgcgt
    17641 tgcgtttggc gtcataaaag cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt
    17701 ttttgaatac tacctcgccc tgagctttgc cgtttttaat acgatccgac agttcaatcg
    17761 gcccgacttg ctgacaagaa atagccgcat cggaggtatc ttccgccaga cccaatcccc
    17821 ctttaatacc acctgttttc gcccggctca cataacaggt gacatttttc acatcgggat
    17881 cgtcaaaggc ttccacaaca attttgtgat ccgggccgat cattttaaat acggtgtcga
    17941 ccgaaccaat ctcttctgca tgagccaatg gccccagcat tatcaggctt aaagacagga
    18001 tcaaatgctt gtatttcata ttgttaccat ttttatctat tacacgagca attagttcgt
    18061 cacggtaaat aacatattga ttaagaacaa aatctcaata cttttatttc cgtcaagccc
    18121 taaaacatac tctactaaga aaaaaacact gaatgctaaa acagcaaaaa atgctattat
    18181 ccaattacct gatgtcaggt gctcgttgtt gaaaggatga ggatatttta tggatcaggc
    18241 cggcattatt cgcgaccttt taatctggct ggaaggtcat ctggatcagc ccctgtcgct
    18301 cgacaatgta gcggcgaaag caggttattc caagtggcac ttacagagaa tgtttaaaga
    18361 tgtcactggc catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt
    18421 cgcactacgc ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc gcttcgactc
    18481 tcaacagaca tttacccgcg cattcaagaa gcagtttgcc cagactcctg cactttaccg
    18541 ccgttctcct gaatggagcg cctttggtat tcgcccgccg ctacgtctgg gtgaattcac
    18601 aatgccagag cacaaatttg tcaccctgga agatacgccg ctgattggtg ttacccagag
    18661 ctactcctgt tcgctggagc aaatctctga tttccgccat gaaatgcgtt atcagttctg
    18721 gcacgatttt ctcggcaacg cgccgaccat tccgccagtg ctctacggcc tgaatgaaac
    18781 gcgtccgagt caggataaag acgacgaaca agaggtattc tataccaccg cgttagccca
    18841 ggatcaggca gatggctatg tactgacggg gcatccggtg atgctgcagg gcggcgaata
    18901 tgtgatgttt acctatgaag gtctgggaac cggcgtgcag gagtttatcc tgacggtata
    18961 cggaacgtgc atgccaatgc tcaacctgac gcgccgtaaa ggtcaggata ttgagcgata
    19021 ctacccggca gaagacgcca aagcgggaga tcgcccaatt aatctgcgct gtgaactgct
    19081 gattcctatc cgccgttaac gctgcagctc atctaatgca ggggcgtcca gatgcgagat
    19141 gtcccctgcg gtttcaacga cccagccaga cgccagccac aggctttcct gataatccac
    19201 gcgcgaaata gaacagttac gcagacgtaa gcggcgctct gcccatgctg gtaatccgag
    19261 aatcgtactc accagacatc ccagtgcaat accatggctc accagcagcg gtcggcttcc
    19321 ctgcggtaag tcccggcaag attccagtgc cgcattgacg cgatcgctga gctcctgcat
    19381 tgactcgcct tcaggaatac gcccgtcaac ggtgccattg accagctgcc gacgccagtt
    19441 ctcttcttct tcggtcagag aatcgatatg tcttttctcc agcacaccca tgtttaattc
    19501 acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt
    19561 acgccgggtg cgtcctaaat cgctactgat aatatgcgta atgccaagct ctttggcacg
    19621 ggttgccacc tgcatcgcct gttgctcacc tttggcggtc agcgggctgt cagactggcc
    19681 ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt atacctgtaa
    19741 catgcttttt ttccgtatac tgctgtaacg ttaattttct gagtaatgct gattatgcac
    19801 caagttatct gtgcgaccac caatcccgct aaaattcagg ccattctgca ggcatttcac
    19861 gagatcttcg gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag cggtgtaccg
    19921 gaacagccct ttggcagtga ggaaacgcgc gctggcgcac gaaatcgggt agcaaatgcc
    19981 cgccgtttac ttccagaggc tgatttttgg gtggcgattg aagctggcat cgatggtgac
    20041 agcactttca gttgggtggt gattgaaaac accagccagc gcggcgaggc gcgttcggct
    20101 actttgccgt tacctgcggt gattctgcag aaagtgcgtg aaggtgaggc gcttggtccc
    20161 gtgatgtcgc gttataccgg tattgatgag attggtcgta aggaaggggc gatcggcgtg
    20221 tttaccgccg ggaaactcac tcgcgccagc gtctatcacc aggcggtgat cctggcgctg
    20281 agtccgtttc acaatgccgt gtatcaggca ttgtaggacg gataaggcgt tcacgccgca
    20341 tccggcattc ggtgcacgat gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa
    20401 tcaatcgctt ttcagcaaca cctcttccag ccactggcgc agctccacgg gcgcggcttt
    20461 caggctgtta gatccacgcg taatcgtcgc gatgcccgcg ccgagttcat tttttaactc
    20521 acgctgactc atttcgccac gcaacagctc ttcgacaata cgcacgcgag tccccaacgc
    20581 ttcgcgctca tctggcgtca gcatcaggtt taacaacggt aaatggagat cgttttggta
    20641 ggcattctta agcaggtcga caaaacgtaa ccactcctgg tgacgctgtt ctgccatcgc
    20701 tgctgaatag ggtgattgtt gggccataat atgtcgccat tgttagcggg ggaagcaaaa
    20761 tgcctccccc ggttgtactc gctaaagagt acgatagcat atcataaacg tgcggatcag
    20821 taacgacgtc cccattccgt ggcgctcatc aacgtcggtt tatcccccat gaaataacgg
    20881 tagtaagcgt cataagccag cacgttcttc acataaccgc gcgtctcgga gaacggaata
    20941 ctctcgacaa atgccacagc gtcgatacgc ccggcgctgt tgccaagcca ggttcgcacc
    21001 cgtccaggtc cggcgttata agctgctgag gagaaaatac ggttattgcc aaactgctga
    21061 taaacatatt gcaggtaact ggtgccaatg ttgatattcg tttccggatc cagcaattgc
    21121 ccagggctgc tataaccagg aatagagaac atcttcaccg tatgggtcgc tgtaccaggc
    21181 ataatctgca tcaggccgct ggcccctacc ggtgatttca ctttcggatt ccaggcgctc
    21241 tcctgacggg caatcgccat cgcatagctt tgcgggatct ctttcccact ggtgtaacgt
    21301 ttgaaaagat cgttgtaagc cagcgggaat cgctcttcca gatgatccca aagcttcccg
    21361 gcgatcgttg cctgaacgct aagatcccac cattggttat tgaaagcata ccgagccagt
    21421 tgagcctgct ctgtttttga cttgctcttc accagattgg cccactcgct acgcgcggtg
    21481 ttatcgagat tccagtacat caactcgcgc acgcgcgcca tctccggccc ctgagtcagg
    21541 gcgctgtcaa cattctgcgg cgctttatca atcttcagct catactcttc tccgatgcgt
    21601 tgtgctgcaa ccatcgggta gaaaccacgc tgttgcatga gttgatgcaa aatctctttc
    21661 gcttcagctt cacgtccgcg ttccagcaat aaatccacct gccagtaacg ccattcatct
    21721 ttctcttttg cttccatcgg cagacgcgcc agccaggtat tcaggccgcg acgatcgccg
    21781 gtgccaagtg ccattcgcac acggcgttca atcaacgacg tggattgcga gcgcataatg
    21841 acgtcatcgc gccatttcgc ctgctcgtcg gtgacatcgt tgcccatcaa acgccaggcg
    21901 acgatatcgc gcagctcctg aatctgatct tcattaagct gctgcacctg ggcaagcgac
    21961 gggatcatca gccgtgcatt ttcagcatcc tgccgcgcca cactggcaaa cgccaccgct
    22021 gccatttgac gggtaaaatc ggtcgctccg gttgtacgcg cgaaggtcag taccgtatta
    22081 gggttgttcg ccagtgaaat gattgccgag gcgatagtct ggtaatcggc aggcatctgc
    22141 cctgccagca ctgttaccag gccagtgtta cccgctttca tcgccagacg gatacgctct
    22201 aaatacgcca gcggatcttg tttacctgac gcacgccaga cgctaaataa cttgtcacag
    22261 gcgttaggct ggctcttgcc ggttagccac agctctttcg ccccttgcca ggcttcttca
    22321 ctctgcccgg tgttccattt cgcatagtag taattacatt gcgcttcggt agttccgggc
    22381 ttttccgggc taaaggctaa caagccacgc cagtcttcac gccgcgccag ttcattgacg
    22441 aaacgagatt gcagcgtgcg agcgggagga agcgtggggt tagcgcgaac aaagttagtg
    22501 accgtcaccg ccggttgatt catcagatca tcggtgatct ggcggtattc caggtaggga
    22561 taaagcggat aatccttcag tccaggcatc atttgttcga ccacatccat ttgtcgatta
    22621 tcccaggcct gcttgatttg cgcgtaacgg ctacgctgct catccagtga gtcggctcgc
    22681 gccacgctgc tgaccgtcag cagacagaca ccggcagcca acagccgcca ggtaacttgt
    22741 ttggcttttt ccacaagcgc atcctctaag tgtaaataca tcaatgcagc gtcatgccgc
    22801 gtaatagatt tatgctaacc agtcattgcc gtttacgcca cgttacggac acttttttac
    22861 ttttactgcg aaggtgatcg accaactggg attaggcggg gaaaaaggct acacttcgcc
    22921 tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct caattcgttt
    22981 ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg aaaaacatct
    23041 ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc gcgggtaagt
    23101 ctaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa gcgcgtccgc
    23161 agccagacat caagattggt tacctgccgc aggaaccgca gctgaacccg gaacacaccg
    23221 tgcgtgagtc cattgaagaa gcggtttctg aagtggttaa cgccctgaaa cgcctggatg
    23281 aagtgtatgc gctgtacgcc gatccggatg ccgattttga caagctggcc gctgaacaag
    23341 gccgtctgga agagatcatt caggctcacg acggtcataa cctgaacgta cagctggagc
    23401 gtgcggcgga tgcgctacgt ctgccggact gggacgcgaa aatcgctaac ctctccggtg
    23461 gtgagcgtcg tcgcgtagcg ttgtgccgcc tgctgctgga aaaaccagac atgctgctgc
    23521 tcgacgaacc gaccaaccac ctggatgccg aatccgtggc ctggctggaa cgcttcctgc
    23581 acgacttcga gggcaccgtg gtggcgatta cccacgaccg ttacttcctc gataacgttg
    23641 caggctggat cctcgaactt gaccgcggtg aaggtattcc gtgggaaggc aactactcct
    23701 cctggctgga gcagaaagat cagcgcctgg cgcaggaagc ttcacaagaa gcggcgcgtc
    23761 gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc cgccagtcga
    23821 aaggtaaagc acgtctggca cgctttgaag agctgaacag caccgaatat cagaaacgta
    23881 acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa gtgctggaag
    23941 tcagcaacct gcgtaaatcc tacggtgatc gcctgctgat tgatgacctg agcttctcga
    24001 tcccgaaagg ggcaatcgtc gggatcatcg gtccgaacgg cgcgggtaaa tcgaccctgt
    24061 tccgtatgat ctctggtcag gaacagccgg acagcggcac catcacttta ggtgaaacgg
    24121 tgaaactggc gtcggttgat cagttccgtg actcaatgga taacagcaaa accgtttggg
    24181 aagaagtttc cggcgggctg gatattatga agatcggcaa caccgagatg ccaagccgcg
    24241 cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgcgtt ggtgaacttt
    24301 ccggtggtga gcgcggtcgt ctgcatctgg cgaagctgct gcaggttggc ggcaacatgc
    24361 tgctgctcga cgaaccaacc aacgacctgg atatcgaaac cctgcgcgcg ctggaaaacg
    24421 ccctgctgga gttcccgggc tgtgcgatgg ttatctcgca cgaccgttgg ttcctcgacc
    24481 gtatcgccac gcacatcctg gactaccagg atgaaggtaa agttgagttc ttcgaaggta
    24541 actttactga gtacgaagag tacaagaaac gcacgctggg cgcagacgca ctggagccga
    24601 agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg cggcgtgaac
    24661 gccttatccg gcctacaaac caagaaattt caatacgttg tgaatctcca ggcctgataa
    24721 gacgcggtaa gcgtcgcatc aggcattgag caccgaatgc cggatacggc gtgaacgcct
    24781 tttccggcct acaacagcct tagtatccgc aatttcgtag cgggtcaccc gtgcaggttt
    24841 tttcaaacct tgtttaatct cgacagcctc ttccagagag gttttcaact catcaaaaaa
    24901 cttattttat acctcgcctt tgagccgttt catcgcggtt atctctgctc ccccatcatc
    24961 tcccgcacca gctccacgca gcgcaggaaa cggctgtcat aatcgtcctc ttcaacccgc
    25021 acgaattcga tattgttctc ttccagcatc tccaccagca agttctggaa ctctttgcga
    25081 tccaccgaac tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt gttctccagc
    25141 aggatcacca gatcgaaacg gtattcatca atcaacgcct gtacgaacgg atgctcacgc
    25201 ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cagtgtcaat aaacgccact
    25261 ttattggcat atttcactgc aaaatcaatg tattgtgcgt ggcccagcgc gattttatcg
    25321 taatcggaat actgcaatgc gatctcatca ccgccgaggt gtgaaaagac ataatcgcga
    25381 ccatattccc acgcactggt ggtattgaag atattggcaa gtttgtttac cagggtggat
    25441 ttaccgctcg actcgccacc aaggatcgcc acggtacgta caaagaacgg cttcacttcc
    25501 gtaggaatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc gctgatactc
    25561 ataaaggtac gtttcggatc gaccagcacc gtatcgatcc ccagatgttc catatactgt
    25621 ggcgcatcgg cttcttccga ggtgtagatc aggtccggct gaatcccctt ttcagccata
    25681 aactttttga tgccgttgct ccacacatcc cagccgtgcg gatacggctc catgccctct
    25741 tcgttgaaag catgaatgcg aatatttttc tgatatttaa aagtttgtaa taaccagcgc
    25801 agacgatccg gcaccgtggg ctgctgcgac atggcgctgt cttcaaacaa cgcgcggtcg
    25861 cgggtatcgt caaaacccat aatgatatgc agctcgtcaa cctggctaca ggcgcgctgg
    25921 ataaggtaga tatgtccggt atgcagcggg tagaacttac cgaatacgac accaatggtt
    25981 ttcttctgtc ggggaaattc aagccccaaa aaacggtgca acgcctccag cttttgcgcg
    26041 ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt cataccgctg
    26101 gcatcagcta cctgctgtag cgtgcagccc tgttgcttga tggcagtttt caggtaatca
    26161 aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg ctaagcgcgt
    26221 cggagagttt tttaacgcca aaaatctgca tcccttccgg cgcttttttc ggtacgttag
    26281 ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttctgaaatt cgttcctgac
    26341 cgctgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc accagatcct
    26401 gtggcagcgg tctgtcacgc aggctggaaa ccatcgccag cagtaacgct aaatcggcac
    26461 tggtttcggt taccttcacg ccgccgacca cgttcacaaa tacatcctga tcggccattt
    26521 gcagaccacc gtgacggtgc aagacagcca gcaggattgc cagacggttt tgttccagcc
    26581 ccactgccac gcggcgcgga ttcgccatca tcgagtgatc gaccagcgcc tgaatctcca
    26641 ccagcaacgg acgcgttcct tcccacacca ccatcacgga gctgccggag gtcacttcat
    26701 ctccgcgact taagaaaatt gccgaagggt tgctgacttc acgcagcccc tgttcggtca
    26761 tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt tttatggctg cgcaaggtgc
    26821 gaaaacggga gtcggcatca ccatccagaa gcaccgaaca gtcgatacag tgttccagca
    26881 ctttagggcc agccagcgag ccatctttgg ttacgtgccc aaccatgaca atcgccacac
    26941 cgcgcgtttt ggcgaagcgc gtcagataag ccgccgtttc acgcacctgc gccacgctac
    27001 caggcgatga ttgtacatcc gccatatgca tcacctggat cgagtcaatt accatcagtt
    27061 tcggttgctc ttcttcggca atcaggcaga tctgttcgat gctggtttcc gacaacatat
    27121 tgagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt tgcagcgact
    27181 cttcgccagt gacatacagc gttttcatct gctgggccag tttgcacagc gtttgcagta
    27241 gcagcgtgga tttccccgca cctgggttac cgccaatcag aatggcgctt cccggcacca
    27301 cgccgccgcc tagtacgcgg tcgaactctt taaatccggt ggaaaaacgc ggcagctctt
    27361 caaggctgat atcggagagt ttctggactt ttgccacccc ggcgctaccg gcatagccgc
    27421 tgagacgctc gttacgcgcc accgttggcg acgcagcaag acgcacctcg gtgatggtgt
    27481 tccaggcatg acaggcactg cactgcccct gccagcgcgg ataatcggcc ccgcattcat
    27541 tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat gctgtgccgc
    27601 ccgcaggatg gcgggcgagc aattacttct gattcaggct gccagagagg atgcagaata
    27661 cccccatcag gtcagcgtga cggatggtga cttccgcctt ttcattcact tttggcttgg
    27721 catggtaggc aatccccagc cctgctgctt tgatcatcgg caggtcatta gctccatcgc
    27781 caatcgccac ggtctgcgcc agcgggattt catactcctg cgcgaggcga gtcaaagttt
    27841 tcgctttgta ctgcgcgtct acgatgtcgc caatcacatt gccggtaaat ttaccgtcca
    27901 tgatctccag ttcattggct accacggcgg tcaggcgcag cttgtcgcgc agatattcag
    27961 caaagaaagt aaagccgccg gaggcaatcg ccactttcca gcccagcgtt tccagcttga
    28021 gcaccagttg cgttaagcct ggcatcagcg gcagattttc acgcacctgt tgcagaatat
    28081 tggcgtcagc gcctttcagc gtcgccacac ggctgcgcag gctggcggta aaatcgagtt
    28141 cgccgcgcat cgcccgttcg gttacttccg ccaccatctc gcccgttccg gccagtttgg
    28201 caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc accagcaaac
    28261 ccggcgtgcg caggtgcggg attttcccca gcggggcgac atccagttgc gcttcatggg
    28321 ccaggcgcgt agcccgtgcg gtgagtgaac ctgccagacg aatcacctga taatcttcca
    28381 cgcaccaggc ggcaacaatc accatcgccg cacccagttt gctctggtat tgggtcagac
    28441 gctgtttatc cagcccacga ccatacagca gccagccgct acgacctgcg tggtaatcca
    28501 gtggcatcac ttcatcacca cttaatgaaa gaggcagacc cggccataaa gagacatctt
    28561 caggcaggtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg ttcgaagcag
    28621 ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta agtctcaaat
    28681 tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc cggctgcatc gggcagtgat
    28741 tgtcctgttc tgtcttgcct tgttagtggc actgatgcag ggggcgtcat ggtttagtca
    28801 aaaccaccag cgacagcgta atccacagct ggaagaactg gcccgcaccc tggcgcgtca
    28861 ggtgacgctg aacgttgcac cgctgatgcg taccgactca ccagatgaaa aacgcattca
    28921 ggcgatcctc gatcagttaa cggatgaaag ccgcatcctc gacgcgggtg tgtatgacga
    28981 acaaggcgat cttatcgccc gttcaggcga aagcgtcgaa gtgcgcgacc ggctagcgct
    29041 cgacggtaaa aaagcaggcg gctattttaa ccagcagatt gtcgagccaa ttgcgggtaa
    29101 aaacggaccg ctcggctatc tgcgcctgac actcgacacc catacgctcg ccaccgaagc
    29161 ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct cactggcaat
    29221 cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc agcaatcgcc
    29281 cttcctgtta accgccagca aaccggtgcc ggaagaggaa gaaagcgaga aaaaagagtg
    29341 acccattact acaagaaagg aaatcgttat gtccacatta cgcctgctca tctctgactc
    29401 ttacgacccg tggtttaacc tggcggtgga agagtgtatt tttcgccaaa tgcccgccac
    29461 gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtgattggtc gcgcgcagaa
    29521 cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc tggcgcggcg
    29581 cagtagcggt ggcggcgcgg tgttccacga tctcggcaat acctgcttta cctttatggc
    29641 tggcaagccg gagtacgata aaaccatctc tacgtcgatt gtgctcaatg cgctgaacgc
    29701 gctcggcgtc agcgccgaag cgtccggacg taacgatctg gtggtgaaaa cagtcgaagg
    29761 cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgtggct tccaccacgg
    29821 cactttgctg ctcaatgccg accttagccg cctggcaaac tatctcaatc cggataaaaa
    29881 gaaactggcg gcgaaaggca ttacctcagt gcgttcccgc gtgaccaacc tcaccgagct
    29941 gctgccgggg atcacccatg agcaggtttg cgaggccata accaaggcct ttttcgccca
    30001 ttatggcgag cgtgtagaag cggaaatcat ctccccggac aaaacgccag acttgccaaa
    30061 cttcgccgaa acctttgccc gtcagagtag ctgggaatgg aacttcggtc aggctccggc
    30121 attctcgcat ctgctggatg aacgctttag ctggggcggc gtggaactgc atttcgacgt
    30181 tgaaaaaggc catatcaccc gcgcccaggt gtttaccgac agcctcaacc ccgcgccgct
    30241 ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc tgcaacaaga
    30301 gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa gagctacggg agttgtcgac
    30361 gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat accaaaggaa gggaatatga
    30421 tcgatctgga aaaccaggag cgggagatta tcaatttaat gttaagccag cgtatttcct
    30481 ggctagccgc cgtgcgtatc aggcataaat tatcacttgc tgaagtaagt aaaatgttag
    30541 gtatctcgat taattctcta aaatagatcg aaaagacgga acggcttagc tcaaacatca
    30601 aaagcaaaat gggagaaatt tatggttgtc caccagagtt gctgatatgc ccttcctgga
    30661 tga
//
LOCUS       DAZOZD010000026        29549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOZD010000026 DAZOZD010000000
VERSION     DAZOZD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.026"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..690
                     /gene="dgt"
                     /locus_tag="ACHUPB_000943"
     CDS             <1..690
                     /gene="dgt"
                     /locus_tag="ACHUPB_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR0757831.1"
                     /translation="LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSR
                     SNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDL
                     LKLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKR
                     FPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAW
                     DEYRRLMAVEQ"
     gene            820..2244
                     /gene="degP"
                     /locus_tag="ACHUPB_000944"
     CDS             820..2244
                     /gene="degP"
                     /locus_tag="ACHUPB_000944"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0757832.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2399..3556
                     /gene="cdaR"
                     /locus_tag="ACHUPB_000945"
     CDS             2399..3556
                     /gene="cdaR"
                     /locus_tag="ACHUPB_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR0757833.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3645..4031)
                     /locus_tag="ACHUPB_000946"
     CDS             complement(3645..4031)
                     /locus_tag="ACHUPB_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0757834.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="ACHUPB_000947"
     CDS             complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="ACHUPB_000947"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR0757835.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="ACHUPB_000948"
     CDS             complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="ACHUPB_000948"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0757836.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="ACHUPB_000949"
     CDS             complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="ACHUPB_000949"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0757837.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8649..9443)
                     /gene="map"
                     /locus_tag="ACHUPB_000950"
     CDS             complement(8649..9443)
                     /gene="map"
                     /locus_tag="ACHUPB_000950"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0757838.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9811..10536
                     /gene="rpsB"
                     /locus_tag="ACHUPB_000951"
     CDS             9811..10536
                     /gene="rpsB"
                     /locus_tag="ACHUPB_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0757839.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10794..11645
                     /gene="tsf"
                     /locus_tag="ACHUPB_000952"
     CDS             10794..11645
                     /gene="tsf"
                     /locus_tag="ACHUPB_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0757840.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11792..12517
                     /gene="pyrH"
                     /locus_tag="ACHUPB_000953"
     CDS             11792..12517
                     /gene="pyrH"
                     /locus_tag="ACHUPB_000953"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0757841.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12809..13366
                     /gene="frr"
                     /locus_tag="ACHUPB_000954"
     CDS             12809..13366
                     /gene="frr"
                     /locus_tag="ACHUPB_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0757842.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13458..14654
                     /gene="ispC"
                     /locus_tag="ACHUPB_000955"
     CDS             13458..14654
                     /gene="ispC"
                     /locus_tag="ACHUPB_000955"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0757843.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14843..15601
                     /gene="ispU"
                     /locus_tag="ACHUPB_000956"
                     /gene_synonym="uppS"
     CDS             14843..15601
                     /gene="ispU"
                     /locus_tag="ACHUPB_000956"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0757844.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15614..16471
                     /gene="cdsA"
                     /locus_tag="ACHUPB_000957"
     CDS             15614..16471
                     /gene="cdsA"
                     /locus_tag="ACHUPB_000957"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0757845.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16483..17835
                     /gene="rseP"
                     /locus_tag="ACHUPB_000958"
     CDS             16483..17835
                     /gene="rseP"
                     /locus_tag="ACHUPB_000958"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0757846.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17865..20297
                     /gene="bamA"
                     /locus_tag="ACHUPB_000959"
     CDS             17865..20297
                     /gene="bamA"
                     /locus_tag="ACHUPB_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0757847.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20419..20904
                     /gene="skp"
                     /locus_tag="ACHUPB_000960"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20419..20904
                     /gene="skp"
                     /locus_tag="ACHUPB_000960"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0757848.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20908..21933
                     /gene="lpxD"
                     /locus_tag="ACHUPB_000961"
     CDS             20908..21933
                     /gene="lpxD"
                     /locus_tag="ACHUPB_000961"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0757849.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22038..22493
                     /gene="fabZ"
                     /locus_tag="ACHUPB_000962"
     CDS             22038..22493
                     /gene="fabZ"
                     /locus_tag="ACHUPB_000962"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0757850.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22497..23285
                     /gene="lpxA"
                     /locus_tag="ACHUPB_000963"
     CDS             22497..23285
                     /gene="lpxA"
                     /locus_tag="ACHUPB_000963"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0757851.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23285..24433
                     /gene="lpxB"
                     /locus_tag="ACHUPB_000964"
     CDS             23285..24433
                     /gene="lpxB"
                     /locus_tag="ACHUPB_000964"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0757852.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24430..25026
                     /gene="rnhB"
                     /locus_tag="ACHUPB_000965"
     CDS             24430..25026
                     /gene="rnhB"
                     /locus_tag="ACHUPB_000965"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0757853.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25063..28545
                     /gene="dnaE"
                     /locus_tag="ACHUPB_000966"
     CDS             25063..28545
                     /gene="dnaE"
                     /locus_tag="ACHUPB_000966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0757854.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28558..29517
                     /gene="accA"
                     /locus_tag="ACHUPB_000967"
     CDS             28558..29517
                     /gene="accA"
                     /locus_tag="ACHUPB_000967"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0757855.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7109 t
ORIGIN      
        1 cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac
       61 gaggcgtggg gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg
      121 gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta
      181 cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg
      241 cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc
      301 gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc
      361 gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct
      421 ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt
      481 ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc
      541 gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac
      601 cgttgccgcc tactgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa
      661 taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt
      721 tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca
      781 cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca
      841 ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct
      901 gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag
      961 gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt
     1021 atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca
     1081 ccgtttcaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa
     1141 cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc
     1201 accaacaacc atgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt
     1261 aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gatccaaatc
     1321 cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt
     1381 gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac ttccgggatt
     1441 gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt catccagacc
     1501 gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg
     1561 atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct
     1621 atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa
     1681 cgcggtgagc tggggattat ggggactgag ctgaactccg aactggccaa agcgatgaaa
     1741 gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa
     1801 gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat cagcagcttt
     1861 gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggctta
     1921 ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag ccagaatcag
     1981 gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa caaaggcaaa
     2041 gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg
     2101 aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg
     2161 cgtaaagtgc tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc
     2221 accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg ggaaggggtt
     2281 ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc tttgggcatt
     2341 tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat
     2401 ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
     2461 catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca gcggcgatcg
     2521 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
     2581 cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga ttaatctacc
     2641 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
     2701 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
     2761 gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga acctgattca
     2821 ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga ttgatctcaa
     2881 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     2941 aatggcggag ttacaacaac tgcaaaatgc gctgactacg cccgagcgta ataatctggt
     3001 ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
     3061 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
     3121 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
     3181 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
     3241 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
     3301 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataatgg
     3361 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggctacgtc
     3421 aaaggcgctg tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact
     3481 gaccgggctt gatttgggta attttgatga caggttgctg ctgtatgtgg cgttacaact
     3541 ggatgaagag cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc
     3601 ccgaagggta gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt
     3661 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
     3721 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
     3781 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
     3841 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttcacgctc
     3901 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
     3961 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
     4021 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
     4081 catggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
     4141 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
     4201 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
     4261 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
     4321 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
     4381 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
     4441 gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg cattgtccgg
     4501 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
     4561 cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt aagtgatttt
     4621 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
     4681 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
     4741 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
     4801 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
     4861 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagccta
     4921 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
     4981 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
     5041 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
     5101 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
     5161 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
     5221 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
     5281 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
     5341 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
     5401 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
     5461 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
     5521 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
     5581 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
     5641 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
     5701 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
     5761 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
     5821 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
     5881 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
     5941 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
     6001 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
     6061 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
     6121 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt
     6181 cggtttcaac agtaaagtga cgtagtttag ccggttgacg acgcggctgt ggtggctgcc
     6241 agctactttg tgtcagtacc tgctccagac caaaccgaat aacctcatga cgatctgcgg
     6301 atagcgggct accatcgggt tccagcacga tgaaggtatc catcgccata ccgtcgcgag
     6361 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
     6421 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
     6481 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
     6541 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
     6601 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
     6661 ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
     6721 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
     6781 cgttggtggc gcaaatgtca gccactgtca ggcataccag atagtgcaga cgattttccg
     6841 tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg
     6901 ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg
     6961 agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt
     7021 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
     7081 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
     7141 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
     7201 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
     7261 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
     7321 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
     7381 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
     7441 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
     7501 ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg
     7561 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
     7621 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
     7681 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg cgattatcgt
     7741 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
     7801 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
     7861 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
     7921 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
     7981 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
     8041 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
     8101 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
     8161 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
     8221 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
     8281 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
     8341 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
     8401 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
     8461 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
     8521 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
     8581 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
     8641 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
     8701 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
     8761 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
     8821 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
     8881 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
     8941 tattcacgaa cgacggagaa gccttctgct tcgataaact tctgaatcgc cgcaccgatt
     9001 tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
     9061 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
     9121 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
     9181 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
     9241 gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg
     9301 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
     9361 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
     9421 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
     9481 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
     9541 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
     9601 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
     9661 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
     9721 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
     9781 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
     9841 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
     9901 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
     9961 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    10021 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    10081 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    10141 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    10201 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    10261 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    10321 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    10381 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    10441 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    10501 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    10561 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    10621 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    10681 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    10741 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    10801 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    10861 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    10921 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    10981 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    11041 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    11101 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    11161 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    11221 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    11281 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    11341 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    11401 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    11461 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    11521 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtggggg
    11581 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    11641 cttaattatc gaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    11701 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    11761 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    11821 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    11881 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    11941 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    12001 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    12061 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    12121 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    12181 tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    12241 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    12301 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    12361 cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga
    12421 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    12481 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    12541 taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag
    12601 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    12661 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    12721 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    12781 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    12841 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    12901 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    12961 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    13021 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    13081 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    13141 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    13201 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    13261 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    13321 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    13381 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    13441 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    13501 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    13561 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    13621 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    13681 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    13741 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    13801 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    13861 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    13921 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    13981 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    14041 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    14101 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    14161 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    14221 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    14281 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    14341 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    14401 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    14461 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    14521 atatcgctgc gttgaattta tctgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    14581 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    14641 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    14701 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    14761 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    14821 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    14881 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    14941 gcaagggagg attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    15001 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    15061 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    15121 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    15181 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagctgggaa
    15241 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    15301 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    15361 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    15421 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    15481 ttactttaca gatgttctct ggcctgattt cgatgaacaa gactttgaag gggcgttaaa
    15541 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    15601 atggggggcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    15661 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    15721 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    15781 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    15841 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    15901 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    15961 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    16021 ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat ggctgctcta
    16081 tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca aattgtttgg
    16141 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    16201 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    16261 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    16321 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    16381 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    16441 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    16501 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    16561 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    16621 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatggccct gattccgctg
    16681 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    16741 catgccttca ataataaatc tgttggccaa cgagcggcga ttattgccgc aggtccggtt
    16801 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    16861 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    16921 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    16981 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    17041 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    17101 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    17161 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    17221 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    17281 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    17341 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    17401 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    17461 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    17521 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    17581 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    17641 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    17701 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    17761 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    17821 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    17881 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    17941 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggca ctcctcagta
    18001 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    18061 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    18121 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    18181 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    18241 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    18301 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    18361 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    18421 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    18481 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    18541 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    18601 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    18661 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    18721 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    18781 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    18841 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    18901 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    18961 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    19021 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    19081 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    19141 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    19201 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    19261 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    19321 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    19381 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    19441 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    19501 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    19561 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    19621 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    19681 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    19741 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    19801 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    19861 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    19921 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    19981 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    20041 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    20101 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    20161 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    20221 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    20281 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    20341 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    20401 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    20461 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag cctgttccag
    20521 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    20581 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    20641 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    20701 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    20761 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    20821 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    20881 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    20941 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    21001 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    21061 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    21121 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    21181 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    21241 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    21301 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    21361 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    21421 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    21481 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    21541 tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt
    21601 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    21661 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    21721 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    21781 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    21841 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    21901 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    21961 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    22021 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    22081 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    22141 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    22201 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    22261 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    22321 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    22381 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    22441 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    22501 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    22561 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    22621 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    22681 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    22741 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    22801 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    22861 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    22921 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    22981 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    23041 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    23101 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    23161 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    23221 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    23281 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    23341 tatcctgggg gccggtttaa tccgcgctct gaaagaacat gtgcccaacg cccgctttgt
    23401 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcttggtacg aaatggaaga
    23461 actggcggta atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    23521 tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc
    23581 gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    23641 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    23701 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    23761 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    23821 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccggg
    23881 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    23941 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    24001 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    24061 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    24121 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    24181 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    24241 gggcagagag ttagttaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    24301 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    24361 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    24421 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    24481 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    24541 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    24601 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    24661 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    24721 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    24781 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    24841 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    24901 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    24961 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    25021 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    25081 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    25141 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    25201 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    25261 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    25321 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    25381 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    25441 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    25501 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    25561 tttctcgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg
    25621 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    25681 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    25741 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    25801 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgt
    25861 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    25921 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    25981 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    26041 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    26101 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    26161 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    26221 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    26281 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    26341 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    26401 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    26461 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    26521 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    26581 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    26641 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    26701 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    26761 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    26821 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    26881 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    26941 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcagtcaggg
    27001 atggtggata actttatcga ccgtaaacat ggtcgcgaag agatttccta tccggacgta
    27061 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat
    27121 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    27181 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta
    27241 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    27301 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    27361 gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta
    27421 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    27481 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    27541 gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga aggtccgatt
    27601 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    27661 gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg
    27721 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    27781 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    27841 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    27901 caggtggtgt tagatgggga acgtgaaacg ttaggtctgt accttacggg acaccctatc
    27961 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    28021 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    28081 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    28141 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    28201 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    28261 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    28321 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    28381 gcccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    28441 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    28501 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    28561 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    28621 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    28681 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
    28741 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    28801 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    28861 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    28921 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    28981 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    29041 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
    29101 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
    29161 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    29221 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    29281 gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg
    29341 aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg
    29401 atggcggtat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga tgtgttaagc
    29461 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    29521 gcaaaagttc tgaaaaaggg tcacttcgg
//
LOCUS       DAZOZD010000027        28525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOZD010000027 DAZOZD010000000
VERSION     DAZOZD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.027"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(293..520)
                     /locus_tag="ACHUPB_000968"
     CDS             complement(293..520)
                     /locus_tag="ACHUPB_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0757856.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            712..1917
                     /gene="csdA"
                     /locus_tag="ACHUPB_000969"
     CDS             712..1917
                     /gene="csdA"
                     /locus_tag="ACHUPB_000969"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0757857.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            1917..2360
                     /gene="csdE"
                     /locus_tag="ACHUPB_000970"
     CDS             1917..2360
                     /gene="csdE"
                     /locus_tag="ACHUPB_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0757858.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2411..3217)
                     /gene="tcdA"
                     /locus_tag="ACHUPB_000971"
     CDS             complement(2411..3217)
                     /gene="tcdA"
                     /locus_tag="ACHUPB_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0757859.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3294..4391)
                     /gene="mltA"
                     /locus_tag="ACHUPB_000972"
     CDS             complement(3294..4391)
                     /gene="mltA"
                     /locus_tag="ACHUPB_000972"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0757860.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4600..4676
                     /locus_tag="ACHUPB_000973"
     tRNA            4600..4676
                     /locus_tag="ACHUPB_000973"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4634..4636,aa:Met,seq:cat)
     gene            complement(4751..6004)
                     /gene="amiC"
                     /locus_tag="ACHUPB_000974"
     CDS             complement(4751..6004)
                     /gene="amiC"
                     /locus_tag="ACHUPB_000974"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0757861.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            6236..7567
                     /gene="argA"
                     /locus_tag="ACHUPB_000975"
     CDS             6236..7567
                     /gene="argA"
                     /locus_tag="ACHUPB_000975"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0757862.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7629..9455)
                     /gene="recD"
                     /locus_tag="ACHUPB_000976"
     CDS             complement(7629..9455)
                     /gene="recD"
                     /locus_tag="ACHUPB_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0757863.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(9455..12997)
                     /gene="recB"
                     /locus_tag="ACHUPB_000977"
     CDS             complement(9455..12997)
                     /gene="recB"
                     /locus_tag="ACHUPB_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0757864.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12990..15878)
                     /gene="ptrA"
                     /locus_tag="ACHUPB_000978"
     CDS             complement(12990..15878)
                     /gene="ptrA"
                     /locus_tag="ACHUPB_000978"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0757865.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16054..19422)
                     /gene="recC"
                     /locus_tag="ACHUPB_000979"
     CDS             complement(16054..19422)
                     /gene="recC"
                     /locus_tag="ACHUPB_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0757866.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19435..19758)
                     /locus_tag="ACHUPB_000980"
     CDS             complement(19435..19758)
                     /locus_tag="ACHUPB_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0757867.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19743..20150)
                     /locus_tag="ACHUPB_000981"
     CDS             complement(19743..20150)
                     /locus_tag="ACHUPB_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0757868.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(20147..20710)
                     /locus_tag="ACHUPB_000982"
     CDS             complement(20147..20710)
                     /locus_tag="ACHUPB_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0757869.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(20701..21171)
                     /locus_tag="ACHUPB_000983"
     CDS             complement(20701..21171)
                     /locus_tag="ACHUPB_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0757870.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(21356..22150)
                     /gene="thyA"
                     /locus_tag="ACHUPB_000984"
     CDS             complement(21356..22150)
                     /gene="thyA"
                     /locus_tag="ACHUPB_000984"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0757871.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22157..23032)
                     /gene="lgt"
                     /locus_tag="ACHUPB_000985"
     CDS             complement(22157..23032)
                     /gene="lgt"
                     /locus_tag="ACHUPB_000985"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0757872.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(23183..25429)
                     /gene="ptsP"
                     /locus_tag="ACHUPB_000986"
     CDS             complement(23183..25429)
                     /gene="ptsP"
                     /locus_tag="ACHUPB_000986"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0757873.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25442..25972)
                     /gene="rppH"
                     /locus_tag="ACHUPB_000987"
     CDS             complement(25442..25972)
                     /gene="rppH"
                     /locus_tag="ACHUPB_000987"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0757874.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(26325..26471)
                     /gene="ygdT"
                     /locus_tag="ACHUPB_000988"
     CDS             complement(26325..26471)
                     /gene="ygdT"
                     /locus_tag="ACHUPB_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0757875.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            26657..27346
                     /gene="mutH"
                     /locus_tag="ACHUPB_000989"
     CDS             26657..27346
                     /gene="mutH"
                     /locus_tag="ACHUPB_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0757876.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            27415..28128
                     /locus_tag="ACHUPB_000990"
     CDS             27415..28128
                     /locus_tag="ACHUPB_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0757877.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            28266..28484
                     /gene="ygdR"
                     /locus_tag="ACHUPB_000991"
     CDS             28266..28484
                     /gene="ygdR"
                     /locus_tag="ACHUPB_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0757878.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         6812 a         7789 c         7144 g         6780 t
ORIGIN      
        1 tagttttttt aatctgagcc attataaatt gtccgttgag cttctaccag caaataccta
       61 tagtggcggc acttcctgag ccggaacgaa aagttttatc ggaatgcgtg ttctggtgaa
      121 cttttggctt acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac
      181 catggtagca aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga
      241 ttaatgtagc accgcctaat tgcggtgctt ttttttacct tgcgatcgcg aattactgat
      301 ccagttcgac catctctttc acgtcagtac ggttgatctg ctgtttgttg ccattagcgt
      361 ctttatacga aatcataccg gtatcgttat cagtctgtgg tttgccgtca gagacgatgg
      421 tacgtccgtc attggtgtgc atcacatagt tcgaaccgga acaggcgctc agggcaaaag
      481 tcagcataca ggcagaaata attgcggcag tctttttcat catcttctcc ttaaagcgat
      541 taataaatct taatagtatt gattaacagg ctaaaattaa cgcctaacac tattcagcat
      601 atgttactta cgcggcttcg ccaggatatc cagataattc tgatggttag cactctcctt
      661 gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt
      721 tttaatcccg cgcagtttcg cgcccagttt cccgcactac aggatgcggg cgtctatctc
      781 gatagcgccg cgaccgcgct taaacctgaa gccgtggttg aagccactcg acagttttac
      841 agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg
      901 cgttatgaag ctgcacgaga gaaagtggcg caattactga atgcaccgga tgataaaact
      961 atcgtctgga cgcgcggcac cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt
     1021 ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg cagaacacca cgctaacctc
     1081 gtcccctggc tgatggtcgc ccaacaaact ggagccaaag tggtgaaatt gccgcttaat
     1141 gcgcagcgac tgccggatgt cgatttgttg ccagaactga ttactccccg tagtcggatt
     1201 ctggcgttgg gtcagatgtc gaacgttact ggcggttgcc cggatctggc gcgagcgatt
     1261 acctttgctc attcagccgg gatggtggtg atggttgatg gtgctcaggg ggcggtgcat
     1321 ttccccgcag atgttcagca actagatatc gatttctatg ctttctctgg tcacaaactg
     1381 tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg
     1441 ccctggctgg gtggcggcaa aatgattcac gaagtgagtt ttgacggctt cacgactcaa
     1501 tctgcgccgt ggaaactgga agcaggaacg ccaaatgtgg ctggtgtcat aggattaagc
     1561 gcggcgctgg aatggctggc agattacgat atcaaccagg cagaaagttg gagtcgcagc
     1621 ctggcgacgc tggcggaaga tgcgctggca aaacgtcctg gcttccgctc attccgctgc
     1681 caggattcca gcctgctggc ctttgatttt gctggcgttc atcacagcga tatggtgacg
     1741 ctgctggcgg agtacggtat tgccctgcgg gccgggcaac attgcgctca gccgctactg
     1801 gcagaattag gcgtaaccgg cacactgcgc gcctcttttg cgccatataa tacaaagagt
     1861 gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg aattattggt ggattaatga
     1921 cgaacccgca attcgccgga catccgttcg gcacaaccgt aaccgcagaa acgttacgca
     1981 ataccttcgc accgttgtcg caatgggaag ataaatatcg ccagttgatc atgctgggga
     2041 aacagcttcc ggctttgcca gacgagttaa aagcgcaggc taaagagatt gccggatgcg
     2101 aaaaccgcgt ctggctggga tataccgtga ctgaaaacgg taaaatgcac ttctttggcg
     2161 atagcgaagg gcgcattgtg cgcggcctgc tggcggtgct gttgactgcc gttgagggga
     2221 aaaccgccgc cgaattgcag gcacagtcgc cgctggcatt gtttgatgag ctgggattgc
     2281 gtgcgcaact tagcgcctca cgcagccagg ggttaaatgc gttaagcgag gcgattatcg
     2341 ctgcgacgaa gcaggtttaa gcatagaacc gggtcggata agacgttcgc gtctcatccg
     2401 acctgattgt ttaaccctga cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa
     2461 ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc gccaaatcca gatgcacaat
     2521 ccatccgctt cggcccttct gccgtggctt tcatcgcaca cacagtaccg tctgactgcg
     2581 ggtacaccag cgcttcagta gaaaacacgc aatcgacgcc gagcttacct ttactgtttt
     2641 tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa
     2701 tcgttttcgc cagatcggta acctgaatct gcgtcgggtc aatctgccca cccgcgccac
     2761 cggtcgtgac cagcgggatt ttattgcgcc gacaataagc aatcagcgcc gctttgggcc
     2821 gtacactatc aatggcatca atcacgtacg aataacccac gctcatatac tgcgctacgt
     2881 tatccggcgt cacgaaatca tccaccaccg ttacacggca ctccgggtta atctggcgaa
     2941 tacgctccgc catgacttcc gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt
     3001 gccgattggt attggtgacg cacacatcat ccatatcgat aagcgtgatt gcgccaatcc
     3061 ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc accgatacca accacacaaa
     3121 tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata cagacgcgct gtgccaccaa
     3181 aacgctgacg ccatgcatca ctaattacca cagacatagc acctcagaat taaaaagggt
     3241 gaggttatcc ctcaccctgt cgtatccgta aaaacggtat acagaataac acatcagccg
     3301 ctaaagacgt tacctgcgcc cggggcggtt ttcagcaccc agacacgtcc atagtggttg
     3361 taccaacctg cgcggtgtcc ggcttccggc ccgatccctt gatagatatc gaagtgttgg
     3421 cctttgattg caccaccgac atccagcgcc accatcagac gcagttcgta ctgaccatta
     3481 aatttgccgt tattatccag caacggcact tctgccagca aggtagtacc tggcggaata
     3541 atggaacgat cagaggcaac tgacgcgcga ccaaccagcg gcaccgcact tgcccctttc
     3601 accggtgcaa aagattgcgg tttaaagaag acgaaagacg ggttctgttc cagcagctcg
     3661 cgaacctcgg cttcactgtg tgtttcgccc cagtgacgaa tcgcctgcat cgacatatct
     3721 tcttttttca cttcgccacg gtcgatcagc accttaccaa tgctgcgata ggcatgaccg
     3781 tttttccctg catagctgaa aaagttaagc ggactgccat caccaaagtc gatataccca
     3841 ctaccctgca catccataat gaagttatcc atcagggagt tactgtaagc gagaatatat
     3901 ttatcactca atgcccccgc gtagatctcc gcacgagacg gcagacgacc acgttttggc
     3961 ggcatacggt aaataggata ctggaactcg ccctggcggg tatggcgcgc ctgaattacc
     4021 ggcgtgtaat agcctgtaaa ctgcacgtta ccatagttgt cggcaccttc catctgccag
     4081 gcatcaatgc cgaactggcg catattgcgg gtatcaccgc ctgcgcgcag ccactcttgc
     4141 accgcgttat aaacattact ctggttgcca tacagacgcg gtgacgaatt acggatatgg
     4201 ttaatttgct cggcaaaatc accggcgtta atcggcgcgc caacggcatc tggctggttc
     4261 accagagaga aaggctgggt aaatttcccg tctttatatt gctgtccgcg atcggttggt
     4321 ttggaagagc aggcggcaag cattgccaca accgtgccca taagaaggta ctttacccaa
     4381 cgtcctttca ttgttctctt cttcaggtta aaaaataagg cgcaacgaag ataacaaacc
     4441 gccgcgttta atgaaatgag cagcctctcc ctgacgcaaa ttttgcacaa aaaataggct
     4501 ttagtgattt gtttttgttc aaaatcatgc cagatccgtg atcggggtaa aaaaaaggtt
     4561 gcatgaaaac gcgagcggag tatagtgcgc atccacggag gcggggtgga gcagcctggt
     4621 agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg caaccaatta
     4681 ttgaacaccc taacgggtgt ttttttgttt ctactctccc ataaaaaaag cgccattcag
     4741 cgccttttta tcatcccctt ctcgccagcg ttgccccatc ggcaaaatac gctttaatcc
     4801 ccgcaagaat agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt
     4861 cctcaacgtt actgataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta
     4921 gtacggcaaa cccggcctgt tcaacttgat ttttatgcag cttgttgatt ttaccgagct
     4981 tattcagcac tgctttacca aacttaaggc tgtcagcaat ggtcagcgac tgcaccatat
     5041 cgaacatggt gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg
     5101 aggcgttctg ggtttgtgcc agatattttg ccgcagtact ggttgcacct ttggttgaga
     5161 gcgcaaacac cgaggaaccg ctcggctggc gactggtaaa ggcgtcggca tggatagaga
     5221 caaacaggtc agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga
     5281 tgtcttcatt gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca
     5341 aacggcgagc tatttgcaat accacatctt tttcgcgcgt tttgtatttc cccaccgcac
     5401 cggagtcttc gccaccgtgg ccagggtcaa gcataatgac aatcggacga tcgcgccctg
     5461 ctttacctgg ttgtggacca ctttgcgctg gcggcacctg cttttcgagg tcgcctttgt
     5521 tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga
     5581 ggtccatcac cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt
     5641 ttacgttttg ctttaattca aaaaccatcc gtacggtttg cgggtcaaat tgcccgacgc
     5701 gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg
     5761 agttcaggtt tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact
     5821 gcttatattt cagctgacga tttgattcta ccgtcacgcg ggtgtagctg gacgcaggcc
     5881 agacgcgcac cgccacgacc tggctaaccg cagccaggct gacctgactt acgctcaata
     5941 gccacatagc gcccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg
     6001 acatgcctct cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac
     6061 cggaaacttt agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta
     6121 tattcacggc attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc
     6181 gaaaaaacag aataaaaata caataatttc gaataatcat gcaaaggggt gtgccgtggt
     6241 aaaggaacgt aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac
     6301 ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt
     6361 ctccagtatc gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt
     6421 ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg catcaccacg aaccgctgta
     6481 tcacaagaat atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg
     6541 aacattgcaa ctggatatta ctgctcgcct gtcgatgagt ctcaataaca cgccgctgca
     6601 gggcgcgcat atcaacgtcg tcagtggcaa ttttattatt gcccagccgc tgggcgtcga
     6661 tgacggcgtg gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatccg
     6721 tcgtcaactg gacagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg
     6781 cgagagcttt aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc
     6841 tgagaagatg attggttttt gctcttcaca gggcgtcact aatgacgacg gtgatattgt
     6901 ctcagaactt ttccctaacg aagcgcaagc gcgggtagaa gcccaggaag agaaaggcga
     6961 ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt
     7021 gcgtcgctgt catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc
     7081 acgcgacggt atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac
     7141 aatcaacgat attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct
     7201 ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagct
     7261 cgataacacg actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga
     7321 aatggcctgt gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct
     7381 ggaacgcatt gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac
     7441 gcgcagtatt cactggttcc aggaacgtgg atttaccccg gtggatattg atttactgcc
     7501 cgagagcaaa aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt
     7561 agggtaagcg atgaaaatcg ccggatgcgg caggcgtaac actcgtgcgt cgcatccggc
     7621 aattacgttt atccccgcga actaaacaac gccgccagac cactgcgccg ctcagtacga
     7681 gtggcgattg ccgcacttaa tatacgctca tcggcataca gcgacagacg gcgacgcgcg
     7741 cgggtcaccg cggtgtatac cagctctcgc gttactatcg gcgtgcgttg gctcggtaaa
     7801 atcaacgccg catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgta
     7861 gtttcgtgct ctggcaagcg actcggttgc acagacttaa tattgccgtc cggcatcgca
     7921 aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac
     7981 ccaagcgcgc tgtcattacg ggcaatcatc accggtcgac cttcgtacca acgagagtgc
     8041 gaatgacgat gaattttgcg cttctgttgc ataaactgct caattcgctc attcagtcca
     8101 gccacgccaa atggcccttc ccgcagggcg cacaaaagtt ggtactcatt gaacgcctga
     8161 atgattaaat ccggctcggc acgcgcttgc agcagatcca gataacgtcc ataacccgca
     8221 agagcttcct caagcatcgc aatataatct tcgccgctct gtaaaagccg tttttcaata
     8281 tcagtaaaat cctgctgaaa aacggttttc actgccgttt tatcaccacg gttgatcgcc
     8341 gcagctaact gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag
     8401 agactgtcgc gcaaagatgc cgcttctgtg ccggttcctg ccggaacgtg agttcccgtc
     8461 aggcggctta gctgcccggc acgctcggcg gtaaagccgg cattggcata agcgcagata
     8521 tcgcccagca cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc
     8581 actcgcgcat gatcgggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt
     8641 gacgcttcat ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa
     8701 cgctggctac ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc ttccggaatg
     8761 cgtctctttt gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt
     8821 aagcgcgcgg cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg
     8881 gccatttgaa ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg
     8941 ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg ctgccgccac tttttgccag
     9001 ttaatttcat cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca
     9061 acctcaatgg catgattcac ctcattgaag aagcgcgcta ccgtccgctc gttacaccac
     9121 atgcgattca aataaagacg atcgccacag aggatcatcg gcgtgggttc atctcccctg
     9181 ctgaccgctt gagaagccag caaggattct tcccaatttt gtagctcacc gatttcactg
     9241 acacaggtcg ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga aagcggcaaa
     9301 cagacgtgtc cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga
     9361 tgttcatctc ccgccacggt cagggcaaat tgcacatcca gcgggcgtag ctgtttgtgc
     9421 tccacagctt ccagtaattg cttttgcaat ttcattacgc ctcctccagg gtcataccgg
     9481 caaacatctc atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatccctt
     9541 gttgcggatg ttctttatca acgccacgca ggaacagata gataacgccg ccaaagtgac
     9601 gatcatagtc gtaatcagca atgcgatggc gcagatagcg atgcagcgcc agggtataaa
     9661 gctgatattg cagatcatag cggtgtgcct gcatcgctgc tgccatagcc tgttgggtgt
     9721 aagccgaact gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt
     9781 cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc gcgtacctgc atgaactcca
     9841 gcggcgggca gccagcggat agcgggtcaa actggcgaat tagtgcatca agctggctgg
     9901 cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc cacctgttta tcgcggtcgg
     9961 aaagctgact caggctaaca cccgtttcat tgagaggtgc ctggaggaca gccgtgatcc
    10021 actcggtcaa caccggttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta
    10081 cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg
    10141 tccccggtga cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct tcaacgacgc
    10201 tgaccacgcc tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac
    10261 cacgctgctg caagccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta
    10321 gtgtcctcgc attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac
    10381 cagtttgtgc cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa
    10441 gccctgccgc atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga
    10501 cgtcggtgtc acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc
    10561 aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt
    10621 cgacctccgc gaggtcgacg ctttctggcg cagcattaag atccaaaact gcctcaaacg
    10681 agtggcgatc gtgataaaac gcctgatcct ggacgcggaa attggtaata aatggcaacc
    10741 agaccagcgg atattccagc cctttcgatt tatggatcgt gacaatctgc accagatgtt
    10801 tatcactttc gaggcgcatt tgttggctgg aggcattact gtctggctcg aggatatgtt
    10861 gcgataacca gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgaagca
    10921 gttcgctgat atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt
    10981 tttctgcaat gttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc
    11041 gccagatttg ccgataacca tcgaactctt cgactaccgc atcccacgca tgttcgtcat
    11101 tattcagcgt ttcaatatcc agcgcattca gccccatcat tgacgttgcc agcgcactgc
    11161 gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa cagccaaagc atttcctgcg
    11221 cttccagagt ttcaaaaaca ctatcgcggt tcgaaaggta aacggaaggg atttccagca
    11281 acgttaaggc atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acgctgatgt
    11341 ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc
    11401 cggcttgtag ccagtcgcgg atttgcgcag cacatacctg cgccatggta ctttgatagt
    11461 cgccaacgcc gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac
    11521 ctttaaagac aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta
    11581 tttcgcgaaa catgaacgcg tcatcagtct ggctgaaaag cttgttcaca ctgttcacca
    11641 ttccaggtgc ggagcgccag ttggtgtcta aagtgtagtg ggcgtgaact tcactacgcg
    11701 ccttcatata agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc
    11761 caatcagcaa caatgcggtt tctggctgat ggtgccagat acggcgaaaa attcggtact
    11821 gctgggggtc ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg
    11881 ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt
    11941 catcaaaacc caattcgcca cggcggcgtt tttcacgcgc tactgtttcg cggatctcag
    12001 ccaatgcgcg ggtgatcacc agatcacgga tcgacaatgg ttctgcaagc agttgatcga
    12061 tcgcctcaaa cagtggatgt cgcggggttt cccccccggc tttcgtgcga tcttctaaga
    12121 aacgctggga gaatttttcc agcgactccg gcaactgata actgtttgtc tcttcttctg
    12181 cccaggcgct gatcttgtcg atccatttag cctgattgct acggttaaac ttgcgtcgat
    12241 caataccaga agattcgatc agcgcatcca gttcacccac tgcgtcgcgc cactgctgtt
    12301 ttaccgtatc aatacgcgcc acaatttgcg cgtgacggga agctagcgtt tcatcatcgg
    12361 gcggcggtgc tttgataacc ggcgcttcgc cttgcagata acgattaata tcgcgcagca
    12421 acgcctgcgg ccctttccag gtttcaaaga cgacctgggc tatttcacgc ggcagcgggt
    12481 agcagtggcg acgccagaaa tcggcgcagg cctggtagcg tagcagagac tcatcttcaa
    12541 tcagctgctg ctcaaacagc atgccggatt caaaggcatt caggttgagc atgcgctggc
    12601 aaaagccgtg aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc
    12661 actgcgcggc ttgcgcttta tcgtcgacct cttccagcag gtgttcgtac aatggattgt
    12721 cggtggtttc acgcagacag gcgatgcgca actcgtggat attgctacgg atacgaccgc
    12781 gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acggtcagcg
    12841 ggcggggaaa ggcggcggaa ccgcctagtc caagtaacag gcgcaaataa agcgccgcaa
    12901 tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag gcgctcgccc tgtaagggca
    12961 agcgcaaagg atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt
    13021 gtttgctgca acgcgctgac gttctcccac actttccagc cttcaggatg cacatattcg
    13081 gctttcccgt tttggctgcc ggaaatctgc gacaggatag ccatgccttg cggctcgact
    13141 accgcctgat ggaagaaatc ggccagtttt tgcggcgtca gcagtttaat ctgggccacg
    13201 attttatcac gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat
    13261 gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg taattaccgc ctgctggatt
    13321 tgcgcaaact catctggctt catcgctcgc aattttgcct ctgcggttgg gaaaaacgcc
    13381 ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag gaagcccatg
    13441 ccccactgac gccccacgct cattggaaac gcaaacacgg catagcccaa ttgttcttcg
    13501 gtacgcaact gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct
    13561 gagctggtgt attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg
    13621 ctgttaccgg ctttttcaaa gatgacggat tgttttttat cgaccactac atctttgttt
    13681 cgacaccact ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc cagcgttgtt
    13741 gcctgggcct cggtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag
    13801 gcgtcgcgat aagccagcac ctctttcaac gtaatggagg gcaaaatttt acgccgttca
    13861 tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg gcataatcgc ctgctcaaac
    13921 gctttaccct tttctgcgga atccatcatc tggttatacc aggacttcgc ctgctcaagc
    13981 tgatcttccg tagcggtata gctaaagtac ccctcgagca atgcctggaa cagctgcggc
    14041 agacgctggg tgtaaccatt agcattaacc ataaggccgt tgttagcgtt ggtggaaaaa
    14101 cttatgccac caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa
    14161 tcattgagcg caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa
    14221 atcaggctga catcagcttt gggttcgctg gaaaaataac ggcttggcgc atacaccacg
    14281 cgcagattcg actcatcaac aatcagctct ggatggtcat atttcttctc tgacttaatc
    14341 agcgagaaat cgtcaggaat ataggggtta agctccggta aggagagcgc aatattggca
    14401 gctttttgct gccagtcagc gaaagtttgt tcgctgattt tatcgacctg atacggcgca
    14461 tcgacaaaat aagccgtttt attgtgcggc tctttcgggc tgatatacca gatacgcgca
    14521 ttctgcggcg tcatcatcgc cagacgttct tttactgctt tagcatcgta ccgatcggca
    14581 atattgactg catccagcgt atgctcaacc ggaacgcgaa tcatggtatc tgccagccat
    14641 tcgacgtaat ccatatcacg ggtgatcgac ggataacgga agtcgatatc cagcacattc
    14701 gccagttcat cgaagtattg tttatcaatg cctttttcac gtaacagatt gagatagcta
    14761 aaaattgccg ccacaacctg atcgcgatta gccaggcctt tatcggttaa agacgcagag
    14821 atcgctaata cgccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca
    14881 actaatccct gcttttgcag ccagtcggaa agtgtacctg ggctgcgatt accaatcaga
    14941 taggtaatca attcatccgt tttactacgg aacttcgctg agttgttatc gatgcgaaac
    15001 tcaacgcgca gaactttacg cggcattgcc gggacgtaat gaatgataat gcccttttgc
    15061 gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga
    15121 ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg gtttattgct gtaaataacc
    15181 gccttcatca gattggcgga atagtacttc tcgtggaaat ctttcagcgc ctgctgcacc
    15241 ggattaccag gtttatcgct taaagtttcg aggttaccac cagaaaactt tgaaccgggg
    15301 tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc
    15361 gccatggtta attcagcgtt caccgcatta cgctcacgtt cggcatattt cttgtcgagc
    15421 aaaggttcag caatagcatc ggccaggcgg tctaccgcac caggcaaggc gtcgttctca
    15481 acttccagat agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc
    15541 attttgagat attcggccag actgtcagcc tgcgggtact ttttcgaccc catcagactc
    15601 atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg
    15661 ggcaccacca gcgccgagag cgatttaact gcctgcggat cagaaaccag caagaccacc
    15721 ataccgttat ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg
    15781 gtttcctgaa tcggctgcca tcccgtttct gcctgactta agggtgccca aagggcaact
    15841 aacaacaata atgctttgaa ccaggtgctg cggggcattc tcggacctca taatcaactt
    15901 aattttctgt ccagattcaa cacgttaacg caaatcatct gccagaattt aatcttgtgc
    15961 tgcacgagtc agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt
    16021 aagcggatag attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg
    16081 taacaggaaa cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg
    16141 ccagagcctt tgataccaga tatcatcacc ttcgccacgc accatcatgt taccttcgta
    16201 agcctgaagg aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc
    16261 gttttgcgcg tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa
    16321 tggcgcggac atcccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg
    16381 ttcggctgca agcggcggaa aacgccactc gccgtctttg cgtagaaaaa ggcgactttc
    16441 accattacca ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc
    16501 cacacttaat aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca
    16561 gccagttatc tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg
    16621 acaggcaatg actctgtcgg caagctgctg catctcctgg cactgagttt cccagaaaat
    16681 ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg aagcgacgga acaagcgttc
    16741 ggcatcatcc tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag
    16801 cccttccaga ataaatggct cggtgtcggg gatttcgctg tcttcagtac ggaagttcac
    16861 ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc
    16921 cagcggcacg gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt
    16981 accagcctgg ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg
    17041 atagttttgt ggatcaaacg gcattcgggt atggaggcaa gtaagatgcg cttttaccct
    17101 tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat
    17161 gtagtcgatc agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga
    17221 acgcccgata tagctgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag
    17281 atagcggtcg tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc
    17341 aaagcccaat ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac
    17401 cactttgaac ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag
    17461 aaaacgctgg ctgatacgtt cttgatccag acgctgtgcc agttcatcac gcaatagtga
    17521 cagcggcacc gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg
    17581 ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgctgag
    17641 catatcgcga caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg
    17701 ccagatgttt agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc
    17761 gctcgattca tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta
    17821 gcccagcaac atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc
    17881 cagctcgcga acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg
    17941 gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc
    18001 cagtaatgcc agcacgtctt ccgacacaaa gcggctgtct ggcaacgata acaggctgat
    18061 aaacgcttcc agtaccggat gcgactgccg cgcccgacgg tcggaaatgg cgtaaggcag
    18121 gtaacgatcc gcaggtgcgc taccaaacac agcccgaata aacggactgt agctgtcgat
    18181 atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc gggtcttctt ccagcatcgc
    18241 cagcaggcga tcgtgcaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaaggt
    18301 gatactgcta tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat
    18361 gttcacacca gcaacggcac ggttttccag ttccagaatg tcagactgaa tgttatgcag
    18421 caggttatct ggcgtgacat cgacaaaggc gtccagctcc tggctgctct ccaggtcaga
    18481 aaggagataa atgtagtcgc gcccaagctt accccatgaa gccagcagcg ggttgccgac
    18541 atcctgttca ccatcgctgt taaagagctg cccggcattt tcgctatcac gaaatagcgg
    18601 caattcgcga tcttcaaaac tgtgtcgacg ctgacgggtc agcagtttcg ccagataagc
    18661 aggatcttta atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc
    18721 aatatgttta cccagcgcct gtagcgcctg gagataaaca ggcggtaacg cggaaatacc
    18781 gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc
    18841 gataaagcgc tgatagagat tggcgcggtg ccagcgcggt tgcccgagtt gatgggtata
    18901 ttccaccagc gccttccaca acggggcttg ccaggcctgt gcttctccca gcccttcaac
    18961 caaatgtcct gtttcccact gtgccagcca gtccggacga tagaccagat actggtcaaa
    19021 caggtccgcc gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag
    19081 ataatgccgc aacagggtaa agtcttcgcg ctccagcaat tgcggcagca gagtcatcag
    19141 tttccagctc atgctctgtt tgttaaaggc gctctctttg gggatttccg gtaacacccg
    19201 gacgaacata tcccagataa agctcgctgg cagcggaaaa tcaatgtttg ccgcaatact
    19261 aaacttttgc gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat
    19321 catctctggt tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc
    19381 ttccagcacg tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg
    19441 acgattcggg caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt
    19501 aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc
    19561 aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca tgccgccaga gctgttggta
    19621 ctggtttctg ctggcgagac tgttcattaa tgttcgctgg atacccgata acgcagtgac
    19681 aatcatcacc atcagcacca tcgcaaccat tacctccggc aggctaaagc cttgttgatt
    19741 cttcagggaa gctgacataa cgccctctct ttcagcggac aaaaatcgct ccagccgcgt
    19801 ggcgaaaaaa caatgtttcc atcaatgact tcgcctgttc gccacaacga aacgccttca
    19861 taaccggcaa ccaataaggc ttcattatct gccagtaaac gcaaacaaac ccgggcatcg
    19921 gttccagcgt actgcgagca ctgaactgcg ggctgcgtct gccaggagtg cattttcccc
    19981 cacgccagcg ccgactgaac gatggcctgg cggcgcaatg actgactttc tatgctcacg
    20041 cgagaggcaa aactgcgatc ctgctgactc attccttgca atagcaagct acccaaaacc
    20101 agcagcatca ggaccagagc cagtgacgaa acaccttttt cgcggttcac aggttgaatc
    20161 ctgtcacgct atagctggca tccaccacgg tttgcggttc agacttactg gcagcacgca
    20221 tattaaccgt caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt
    20281 cgataatgat ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg
    20341 caccgcgtag cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcagactctt
    20401 ttaccggttc gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt
    20461 caccctgtcc gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc
    20521 tctggagatg cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agcgccagct
    20581 tacgggtgct cgttaaactt tcacgctgta acgcaggcag aaagcgtgca gcccccagca
    20641 acaatacgct actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt
    20701 ttacaggcat ccttctgttt ctccttgctg acaaagccgg agtcttcccc acggcgaaac
    20761 caccagccac cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt
    20821 gcgcaggcca aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt cgggccagcg
    20881 tggcacaaag accaatggtg aactgccatg acaggtattg gccccagcag cggaactcac
    20941 aaggcaccat aacgtcccct ccctgataac gctgatactg tggtcgcggt tatgccagtt
    21001 ggcatcttca cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccatagccg
    21061 ttgcgactgc tgccagtatt gccagccata gagtccactt gcgcttagca tgaccaaaat
    21121 cagcatcgcg accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt
    21181 atggagcgag gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc atcttccgga
    21241 ctatttacgc cgttgcagga cgttgcaaaa tttcgggaag gcgtctcgaa gaatttaacg
    21301 gagggtaaaa aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga
    21361 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    21421 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    21481 gttcgcggct taattgcaga tgagtttgat ccatatgatt gctgtacagg tgcgtatcgc
    21541 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    21601 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    21661 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    21721 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgggaat
    21781 ccgggtcgtt tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatctg
    21841 gcgttggcca ggcgcgccac tgtttaccat acactggtcc gaggtcgccg ttttcatcgg
    21901 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct
    21961 gcagaaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgttta gttgtcacca
    22021 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    22081 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    22141 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    22201 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    22261 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    22321 acgcgccgta gccaatcagg aacaagccag agacagctcc cattgggcgc ggtttacgaa
    22381 tatacaggtt gaggataata aacagcacca caccttccag cagcagctcg taaagctgtg
    22441 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    22501 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    22561 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    22621 caaacggaat gagtggtgcg ataaaatcag aaacctggaa gaaggaacgt ttagtacggc
    22681 gggcaaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    22741 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    22801 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    22861 caacttcatt tttggtccag ccgctgcccg gacgattcgc ccgtcgtgtt gccagccaca
    22921 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    22981 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    23041 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    23101 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    23161 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    23221 gctccataaa ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac
    23281 gctgcgcaag attttctgct tcggcataat caatgcgccg cagcaggtat tttacccgcg
    23341 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    23401 acatgggatc gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt
    23461 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg
    23521 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    23581 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    23641 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    23701 gtccggcacg ttcaatcagg cggcgtgctt catcaacttc atcgaggctt gtgaccatcg
    23761 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    23821 tcaagaagat ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat
    23881 tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    23941 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    24001 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    24061 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    24121 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    24181 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    24241 gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg
    24301 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    24361 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    24421 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    24481 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    24541 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt
    24601 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    24661 cgcgacgcag ccgggtgtcc gaaagtaggt gcgagtaaag atcgaaaata gccgccgttt
    24721 ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    24781 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    24841 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    24901 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacaaggca gtcaactgcg
    24961 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    25021 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    25081 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt
    25141 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    25201 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    25261 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    25321 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    25381 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    25441 cttaacctct tttacgtcga taagcagatg tgttttgtgg ttttggcgta ttttcctgca
    25501 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    25561 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    25621 tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag agaaaccatt
    25681 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    25741 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    25801 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    25861 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    25921 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    25981 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaagaagac ggtaaaccac
    26041 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    26101 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    26161 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
    26221 tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa
    26281 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
    26341 ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg
    26401 cacgtcaagg ctgtaaatgg aaacagtagt ggaggttttt cacagttatc ccagctttct
    26461 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg
    26521 cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac
    26581 aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
    26641 accaaggtat catgacatgt cccaacctcg cccactcctc tctcctcccg aaactgaaga
    26701 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
    26761 tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
    26821 ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg
    26881 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
    26941 ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca
    27001 caagctcaaa cgcgtactgt ggataccggt tgaaggtgag cgcagcatcc cgctggcgca
    27061 gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg
    27121 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc
    27181 tcgtcacggg gagtatttac agatacgacc gaaagcagcg aatgcgaaag cgcttaccga
    27241 agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa
    27301 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
    27361 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
    27421 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
    27481 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gctccccaca
    27541 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
    27601 ctgctggcat caatcgcctg ggttacgcgc ctgacaaatc cgctttttac aatattcagt
    27661 caggaaattt ccgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa
    27721 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
    27781 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac
    27841 tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc cgccgtggtg
    27901 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat
    27961 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tcggctttac cctgattctg
    28021 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
    28081 gcggttgaaa gcctcaacct gattcgcaac aaaaagaacc cgctctgata ctccgtacgc
    28141 tctcctgcgg gagggcgtac tcactcctgt taccctcaca gtttaagatt ttcctgcttt
    28201 caagatatat aacgtcggtt tataaacaga ctattatcat aggtgggcca aatgaggata
    28261 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
    28321 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
    28381 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
    28441 tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat
    28501 ccggctgtaa tgccgatcag ttaag
//
LOCUS       DAZOZD010000028        28300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOZD010000028 DAZOZD010000000
VERSION     DAZOZD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.028"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHUPB_000992"
     CDS             <1..468
                     /locus_tag="ACHUPB_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0757879.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHUPB_000993"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHUPB_000993"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0757880.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHUPB_000994"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHUPB_000994"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0757881.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUPB_000995"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUPB_000995"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0757882.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHUPB_000996"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHUPB_000996"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0757883.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHUPB_000997"
     CDS             complement(4084..5313)
                     /locus_tag="ACHUPB_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0757884.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHUPB_000998"
     CDS             complement(5377..5817)
                     /locus_tag="ACHUPB_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0757885.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHUPB_000999"
     CDS             complement(5922..6692)
                     /locus_tag="ACHUPB_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0757886.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHUPB_001000"
     CDS             complement(6689..7615)
                     /locus_tag="ACHUPB_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0757887.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHUPB_001001"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHUPB_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0757888.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUPB_001002"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUPB_001002"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0757889.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUPB_001003"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUPB_001003"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0757890.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUPB_001004"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUPB_001004"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0757891.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHUPB_001005"
     CDS             13322..13669
                     /locus_tag="ACHUPB_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0757892.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHUPB_001006"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHUPB_001006"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0757893.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHUPB_001007"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHUPB_001007"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0757894.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUPB_001008"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUPB_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0757895.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUPB_001009"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUPB_001009"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0757896.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHUPB_001010"
     CDS             18978..20735
                     /locus_tag="ACHUPB_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR0757897.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUPB_001011"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUPB_001011"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0757898.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUPB_001012"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUPB_001012"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0757899.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUPB_001013"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUPB_001013"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0757900.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUPB_001014"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUPB_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0757901.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         6834 a         7690 c         6950 g         6826 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaatt
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac
//
LOCUS       DAZOZD010000029        28105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOZD010000029 DAZOZD010000000
VERSION     DAZOZD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.029"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUPB_001015"
     CDS             complement(<1..215)
                     /locus_tag="ACHUPB_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0757902.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUPB_001016"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUPB_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0757903.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUPB_001017"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUPB_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0757904.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUPB_001018"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUPB_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0757905.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUPB_001019"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUPB_001019"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0757906.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUPB_001020"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUPB_001020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0757907.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUPB_001021"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUPB_001021"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0757908.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUPB_001022"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUPB_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0757909.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUPB_001023"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUPB_001023"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0757910.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUPB_001024"
     CDS             6526..6744
                     /locus_tag="ACHUPB_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0757911.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUPB_001025"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUPB_001025"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0757912.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUPB_001026"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUPB_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0757913.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUPB_001027"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUPB_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR0757914.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUPB_001028"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUPB_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0757915.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUPB_001029"
     CDS             10175..12088
                     /locus_tag="ACHUPB_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0757916.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUPB_001030"
     CDS             12088..13110
                     /locus_tag="ACHUPB_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0757917.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUPB_001031"
     CDS             13104..13322
                     /locus_tag="ACHUPB_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0757918.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUPB_001032"
     CDS             13376..14245
                     /locus_tag="ACHUPB_001032"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0757919.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUPB_001033"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUPB_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0757920.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUPB_001034"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUPB_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0757921.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="ACHUPB_001035"
     CDS             15689..17779
                     /locus_tag="ACHUPB_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0757922.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUPB_001036"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUPB_001036"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0757923.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUPB_001037"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUPB_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0757924.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUPB_001038"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUPB_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0757925.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUPB_001039"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUPB_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0757926.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUPB_001040"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUPB_001040"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0757927.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUPB_001041"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUPB_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR0757928.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUPB_001042"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUPB_001042"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0757929.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUPB_001043"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUPB_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0757930.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUPB_001044"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUPB_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0757931.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUPB_001045"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUPB_001045"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0757932.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6790 a         7559 c         7112 g         6644 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agaga
//
LOCUS       DAZOZD010000030        27804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOZD010000030 DAZOZD010000000
VERSION     DAZOZD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.030"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..335
                     /gene="tnpA"
                     /locus_tag="ACHUPB_001046"
                     /pseudo
     CDS             1..335
                     /gene="tnpA"
                     /locus_tag="ACHUPB_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            395..469
                     /locus_tag="ACHUPB_001047"
                     /pseudo
     CDS             395..469
                     /locus_tag="ACHUPB_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(466..1789)
                     /locus_tag="ACHUPB_001048"
                     /pseudo
     CDS             complement(466..1789)
                     /locus_tag="ACHUPB_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1789..3405)
                     /locus_tag="ACHUPB_001049"
     CDS             complement(1789..3405)
                     /locus_tag="ACHUPB_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0757933.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3701..4417
                     /locus_tag="ACHUPB_001050"
     CDS             3701..4417
                     /locus_tag="ACHUPB_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0757934.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4414..6075)
                     /locus_tag="ACHUPB_001051"
     CDS             complement(4414..6075)
                     /locus_tag="ACHUPB_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0757935.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6271..6699)
                     /gene="ibpB"
                     /locus_tag="ACHUPB_001052"
     CDS             complement(6271..6699)
                     /gene="ibpB"
                     /locus_tag="ACHUPB_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0757936.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6811..7224)
                     /gene="ibpA"
                     /locus_tag="ACHUPB_001053"
     CDS             complement(6811..7224)
                     /gene="ibpA"
                     /locus_tag="ACHUPB_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0757937.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7218..7290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7530..7862
                     /locus_tag="ACHUPB_001054"
     CDS             7530..7862
                     /locus_tag="ACHUPB_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0757938.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7864..9078)
                     /locus_tag="ACHUPB_001055"
     CDS             complement(7864..9078)
                     /locus_tag="ACHUPB_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0757939.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9179..10243
                     /gene="cbrA"
                     /locus_tag="ACHUPB_001056"
     CDS             9179..10243
                     /gene="cbrA"
                     /locus_tag="ACHUPB_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0757940.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10240..11577)
                     /locus_tag="ACHUPB_001057"
     CDS             complement(10240..11577)
                     /locus_tag="ACHUPB_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0757941.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11652..12800)
                     /gene="dgoD"
                     /locus_tag="ACHUPB_001058"
                     /pseudo
     CDS             complement(11652..12800)
                     /gene="dgoD"
                     /locus_tag="ACHUPB_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12797..13414)
                     /gene="dgoA"
                     /locus_tag="ACHUPB_001059"
     CDS             complement(12797..13414)
                     /gene="dgoA"
                     /locus_tag="ACHUPB_001059"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0757942.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13398..14276)
                     /gene="dgoK"
                     /locus_tag="ACHUPB_001060"
     CDS             complement(13398..14276)
                     /gene="dgoK"
                     /locus_tag="ACHUPB_001060"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0757943.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14273..14962)
                     /gene="dgoR"
                     /locus_tag="ACHUPB_001061"
     CDS             complement(14273..14962)
                     /gene="dgoR"
                     /locus_tag="ACHUPB_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0757944.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14931..15563
                     /locus_tag="ACHUPB_001062"
                     /pseudo
     CDS             14931..15563
                     /locus_tag="ACHUPB_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15609..16421)
                     /gene="yidA"
                     /locus_tag="ACHUPB_001063"
     CDS             complement(15609..16421)
                     /gene="yidA"
                     /locus_tag="ACHUPB_001063"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0757945.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16536..16934)
                     /locus_tag="ACHUPB_001064"
     CDS             complement(16536..16934)
                     /locus_tag="ACHUPB_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0757946.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17174..19588)
                     /gene="gyrB"
                     /locus_tag="ACHUPB_001065"
     CDS             complement(17174..19588)
                     /gene="gyrB"
                     /locus_tag="ACHUPB_001065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0757947.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19617..20690)
                     /gene="recF"
                     /locus_tag="ACHUPB_001066"
     CDS             complement(19617..20690)
                     /gene="recF"
                     /locus_tag="ACHUPB_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0757948.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20690..21790)
                     /gene="dnaN"
                     /locus_tag="ACHUPB_001067"
     CDS             complement(20690..21790)
                     /gene="dnaN"
                     /locus_tag="ACHUPB_001067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0757949.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21795..23198)
                     /gene="dnaA"
                     /locus_tag="ACHUPB_001068"
     CDS             complement(21795..23198)
                     /gene="dnaA"
                     /locus_tag="ACHUPB_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0757950.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23495..23575
                     /locus_tag="ACHUPB_001069"
     CDS             23495..23575
                     /locus_tag="ACHUPB_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0757951.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23805..23945
                     /gene="rpmH"
                     /locus_tag="ACHUPB_001070"
     CDS             23805..23945
                     /gene="rpmH"
                     /locus_tag="ACHUPB_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0757952.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23962..24321
                     /gene="rnpA"
                     /locus_tag="ACHUPB_001071"
     CDS             23962..24321
                     /gene="rnpA"
                     /locus_tag="ACHUPB_001071"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0757953.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24285..24542
                     /gene="yidD"
                     /locus_tag="ACHUPB_001072"
     CDS             24285..24542
                     /gene="yidD"
                     /locus_tag="ACHUPB_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0757954.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24545..26191
                     /gene="yidC"
                     /locus_tag="ACHUPB_001073"
     CDS             24545..26191
                     /gene="yidC"
                     /locus_tag="ACHUPB_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0757955.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26297..27661
                     /gene="mnmE"
                     /locus_tag="ACHUPB_001074"
     CDS             26297..27661
                     /gene="mnmE"
                     /locus_tag="ACHUPB_001074"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0757956.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6689 a         7523 c         7094 g         6498 t
ORIGIN      
        1 gacgtcagat ttttgactat gacgcgacag aaaaactacg cacttacttt tcaaatgtat
       61 gtgctgattt tgaagctgaa ctggtttaaa tggatggcga accagatcac gtccatttgt
      121 taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcgtat
      181 cgggtaggtt actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
      241 ttttgtggag tcctggctat tttgccaata gctgcggagg tgcgccaata tccgtcatcc
      301 gccaatacat tgaacaacag caaacacctg gttaggtgga aaaccgcgcc ttatatcccc
      361 gccctgaagg acggggtttt acggcgcacc ggataaattt ggttatgcgc cgtcagccgt
      421 gctaaagaat acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg
      481 ccagtacgct ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct
      541 cggtaccgtt ttcgataacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca
      601 ggcgtccacg accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg
      661 cgggatatcg ccgaccacca gtggttctgg tccgttgtgc ccgaccagac acgggatctc
      721 gaccatcgct tcatcatcaa agttatggat tgccccgttg ttaggcacaa tcagcaacat
      781 ccgctcctga gtgttgaagg caattgccgc cgccagatcg acgatgtatg acgcatgttc
      841 gtcaatctcc agcttggccg ccgctgaatt tcctgccgct gtaatggcgc ggcaagcatc
      901 gaaaacctgt ttctcgcgat gttccattca cctcattcgc gcgggtatgt tcagggttgg
      961 aatgctgaac aacataatcc gggaagagat aatatttcag ataggtgttc ggcaatgtat
     1021 ccgggtccag tgcctggaca tcccgcgctt ttgcataggt gtcgttccag ctcgcttcag
     1081 tatgtgtatc ttgctgcggt ggaacataac cgtgcttcga gacatattgc cgcagctggg
     1141 gcatcaggtc gttgccctgc aaatcttcaa ttgatgtcca ccagccaaag tggttcaggc
     1201 cgtagtagcg cacgcgcatc tgtttgcgat cttgcagccc aacaatttgc gccatccggc
     1261 tttcaatacc gattggcatg tcacagatgt tgaggatttt cgcattcggg cgcagacgac
     1321 gcgtggcttc tgcgacaatg gctgccgggt tggagtagtt gagcatccag gcatttggtg
     1381 aatatttttc catataatcc accagttcca ggacgccgcc aatggaacgc atgccgtacg
     1441 ctattccgcc gggtccgcaa gtttcctgac caacaacacc gtggcgcagt gggatttttt
     1501 catccagttc gcgcatcggg tacttgccga cgcggatgtg cgccatgaca aaatcaacgt
     1561 cgctgaacgc cacttcagga tcggtggtgt aactaaacgc aatgtccggc gctttctctt
     1621 taaggatgac tttacaggct tcggcaatca cttcctgccg cgcgccatcg ttatcataaa
     1681 atttcagtgc acgaagcggg aaacgctcct gattcgccag gagcatcaac acgatgcctg
     1741 gcgtaaaggt gcttccaccg cctgcgacaa ccactgagaa tttggtcatt atactgcctc
     1801 cgtaatggca acattttctg ctgattgatg agaattaatt aagctatcga gctgttcacg
     1861 cagctgggat acatgcagac caatgatcac ctgaatggca tcgccactac ggaagacgcc
     1921 gtgcgctccc agctttttaa agacttcgtc atccagcgtt tgtgacatgt catgcagtgc
     1981 aatgcgtaga cgcgtcgcac aattgttaat gctggagata ttgccgaccc cgcccagggc
     2041 ttgcaggata ccggcagcct gatccagctc tttttttggc tctgccgcgg tggtttggcc
     2101 tcgcgaggct ttgtattcgg cttttgagta gagtttcact tccgcatctt cacgtcccgg
     2161 cgtgcacatg ttgaactgca gaatcagggt gcggaaaacc acgaagtaca gcagggtaaa
     2221 gcacaaccca atggcgattt gggtcagcat catatccgcg tggttgctga acatcgggat
     2281 ccagttttgc ggtaaaacct ggtcaatcag acctccgccc atgttgccca ccacaccaaa
     2341 gagatacatc acggtcgaca ttgaggccgc aagcacagcg tgtaccgcaa acagcaacgg
     2401 tgaaatgaac aggaaggtaa attccagcgg ttcggtaatt cccaccagca tggcggttaa
     2461 ggttgcggga atcagcaagc ccgccacttt tacccgattt tccggtgcgg cagtgaagta
     2521 catcgctaaa gaaatgccca cggcaccaaa gatttttgag ttaccgtgca gggcaaaacc
     2581 gccttccggg aacaacgatt tcagcggctc ggcgctcaaa ctgaactctt gcagatgctg
     2641 cgcccagtac atctgaatgc cgccttcaac agctgccgga cccaagataa acggtccgta
     2701 gatgaagtgg tgtaaaccgg ttgggatcag aatacgttcg aggaaggtgt aaacccagac
     2761 cccaagcgca cccgccgaac gcaggaacgc ttgcagagat tcaatccccg tttgtacttt
     2821 tggccagccg agcaatgtca gccaggcaca ggggatcatc accaggaagg cgataatcac
     2881 cacataagac gtcccctgga aaatgccgag aaaaaccggc agttttttat cgaacagacg
     2941 gttatgcagc gccgtcacaa tgccggaaat gataattgcg ccgataatgc tggtatcgag
     3001 ggttttaatc ccggccatca ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc
     3061 gacgccgaag tagcttcccc aggtcattcc catcgcgttg atgaaatagt tccaggtcag
     3121 gaaactcacc atcaccgcca gacaagcacg tccctgcgct tgcttagcaa ggccaatggg
     3181 taaaccgaca gcaaaaatca gcggcatatt acggaatacc gtccaaccgc cctcttcaat
     3241 aatgtgtacg atttgcgcga ataaactgtt cggatcggtc agtgattccc cgacaaacat
     3301 cgggttttgc agcaagatgg caagacccac cacaatcccg gcgaagggaa acagcagcac
     3361 tggcgtaaac atcgcgccgc caaagcgttg aatttgactg agcatttcca catcctcttt
     3421 tctcaattct gtaagtagga agcctgattg gttatgcggc aggcctggag caaggatagg
     3481 aagatgcgat cggcagaaca tgacgaccca atgggattcg tgatcgcttt catgcttttc
     3541 gttgggcggt agagatctac gttttcgcga aaaagtagac atctgtgagc acaggtacac
     3601 agcatcgaac acatctttaa aaaaaagatg ttttttcaat cgattaagca gaacttgtgt
     3661 gcgcattacc cgggcttgca ggcaaaaaag agatctagag atgatctaca aaagcattgc
     3721 ggagcggtta agaattcgac ttaactccgc agatttcacg ctaaacagcc ttcttccggg
     3781 tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg accatccgta aagccattga
     3841 cctgctggta gcgtgggggc tggtggtccg ccgccacggt agtggcactt acctggtgcg
     3901 caaagatgta ctgcatcaaa ccgccagtct gaccgggctg gtggaggtgt taaaacggca
     3961 gggaaaaact gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc ctccggccat
     4021 tgccagccag ttacggattc aaatcaacga gcagatctac ttctcccgtc gcgttcgttt
     4081 tgtggaaggg aaaccgctga tgctggaaga cagctatatg ccggtaaaac tgttccgtaa
     4141 tctttcgctg caacatctgg aagggtcgaa gtttgaatat attgaacaag agtgcgggat
     4201 tttgattggc ggtaattatg aaagcctgac gccggtgctc gccgatagac tgctggcgcg
     4261 gcaaatgaag gtagcggaac acacgccact gctgcggatc acctcgttgt catatagcga
     4321 gagcggggag tttttgaatt attcagtgat gttcagaaat gccagcgaat accaggtgga
     4381 gtaccattta cggcgactcc acccggaaaa gagttaaccg atactccaga agagcaccgc
     4441 cagtaattgg ggggtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga
     4501 gagcgccgcc gcaccgctag ttggatgaag attattagca aacgccagcg ccggaggatc
     4561 ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg
     4621 cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca
     4681 gcttagccct tcgccattga ccagggtatt cacaaaatcc ccaccagatt tcagaccaac
     4741 gaccgagagg aacagcacga tccccagctc ccgcagcgcg aggttggcac ttggcggcat
     4801 aaaccagtac agcttgccga tactgccgat acgcccgagg atcaacgcca taatcagcag
     4861 accgcccgcc agccccagtt tcaacgcggc cgggaatccc ggcacaaaga cgggaataga
     4921 acctaacaat acgccaagcc cgatgccaat aaacactggc agcatctgaa cctgttgcag
     4981 tttttgctgc gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac
     5041 caggttgagg atatcgccga actgcaggct gatatcgcca ctggcgacca gttcgacccc
     5101 ggcacggttc aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat
     5161 acgttttccg agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgtc
     5221 tttcgtggac agcgaagtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc
     5281 cgctggctga cccaccagat gcagcaaatc gcccagttgg ataatggtat ctggcgaagg
     5341 aacttttagg gtttcttcgc gtttcagacg cgagcagata attttgtcgc cgttgagaat
     5401 cggtacatct ttaatggcta aatcatgcag gttagggttc tcaacgcgaa tattgatagt
     5461 cttgatcagc gcgccgccat tggtgcgtga agactcgtgc tgctgagctt ctgtctcgac
     5521 attgacgcgg aaaataaccc gcaacatcca catggtgaac aaaatcccgc aaatgccgaa
     5581 tggatacgcc atcgcgtaac tcatccccat ctgatcgacc atttccattg gtgtacccag
     5641 gtcgcgcaaa atctgctgcc ctgcccccag cgctggcgta ttggtaaccg caccggagaa
     5701 aatccccagc actaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag
     5761 accaccgatg atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa
     5821 tgaggcaaag aagcccggcc ctacctgaat cccgatggta taaacgaaca ggatcaggcc
     5881 aaattcctga ataacatgca gcatatcgct acttaatgtc atccccgcct gagaaacaaa
     5941 atggccgacg atgatcccac caaacagcac gccgccaata cctaatccta tgccgcgaaa
     6001 tttgacgttg ccgataaaca aaccgacgac tgccaccaaa gccagaatac tgaccgttaa
     6061 tgctatatca ctcatcgtcc atttctcttg agataacatt ttaattgcta cggattatgg
     6121 ctgaatcggt gaagcagatt tgccttatag cgcacattat tacgggaaag aacgtgccga
     6181 aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca
     6241 ccagaatggc ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat
     6301 agcgatacgc tgcgctgcga tgggttcagg ctcattacga attaaatcaa tatgcagtaa
     6361 accgtttacg aaagttgcgc cagagacttc catattttca gccagcgtaa agctcaggct
     6421 aaatggctga ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg
     6481 cgtgcctttt acgctcaggc gcgtaccttc cagttgaatc tctaaatctt cctgacggaa
     6541 acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg
     6601 cgggaagctc tgactttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat
     6661 ccattgacgc atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa
     6721 gcgagtaagt acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga
     6781 tgtcaggccg cgccaggcgg ccttaaggaa ttagttgatt tcgatacggc gcggtttttt
     6841 cgcttccgga atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcgcc
     6901 acgaacatga atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc
     6961 ctggtacaga taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag
     7021 attatcctgg gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat
     7081 gcggtaatgg ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg
     7141 gctctggttg ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag
     7201 cggggataaa tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac
     7261 cttccacaat ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcgactac
     7321 gatgtaaaaa tgggtctgga aatgaacttt tcaagcctta tcagcggact tttttttgca
     7381 gtttatggtc tattgcatag actgaggggg cagcacgaaa cgttaaagtg tgatagccgc
     7441 cacagagcga cgtaatgatg atgttatttt cagcaaggga tcgctataac tcatcaggcc
     7501 aacacccacg atcaacagat gaatttatta tgataagaaa tgtgttgtta gcgttcatga
     7561 tatgcagcgg aatggcatta ctcggaggat gctccagcgt gatgtcccat accggaggga
     7621 aagaaggaac gtatcccggc acccgcgcca gcgccaccat gattggcgat gatgaaacca
     7681 actggggcac taagtcgctg gcgattctcg atatgccgtt taccgctgtg atggacacgc
     7741 ttttattacc gtgggatgtg ttccgcaaag acagctcggt gcgctcacgc gttgaaaaaa
     7801 gcgaagccaa cgcgcaagcc accaatgcgg ttattccacc ggccagaatg cctgataact
     7861 gatttagcga tcagtttcgg tgatccacag ctggccacct tccatccacg ccagccattg
     7921 accatccggc gagaagacca cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa
     7981 atactcaacc tcgccgcttt gcgcatgggc acaagcaatt cgattatcca gcacaaagcc
     8041 caaccattct cctgacggat gccagttaaa tgcagactga atatccgttt tgttatgggt
     8101 taactggcgc ggctcgccgc cctgtggcga gataagccac agttgcacaa tgccgttatc
     8161 atcacgcatt aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg
     8221 gacgttgact aaccccggat aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac
     8281 gccacgcggt ggcgcgggca gggtggtttc cgttccactt aacggcgcat cacctgccgc
     8341 tttccagcca gcttcatctt gcggcaactc aacgataaac agctccggca ctttctcacc
     8401 ctttggcgaa agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc atccttcttc
     8461 ataagcacga ttgatttcat cactgccagg ctgcggcgtg ggcgtggttt tactcaccag
     8521 cacacaccag tggctgccgc tgtattcacg cggatgctgc ttttgtacgt tgaccggacc
     8581 aaacggcgca gcaacaccga cgtttcgcaa atccagcgcc ggatcgagtt catgcattac
     8641 atggtcgtta taggtaaagc tcaccctttc accgttcggg ctaaagacat gcacatggct
     8701 gccgccgcgc agcgctcctg gcgtatacgg cgcggtaata tccattgcat cgagattgct
     8761 caccttgccg ccttcagcaa tgactccgcg ccgatgatgg aagtcgtaat gccatgtttc
     8821 atcaggattt tccgggccgt gaataaagac atatttctct gacttcggat gaacggtcac
     8881 cacgccgacg tgtgcgccct gtgacgcaca gtagataacc tcgacctcac cggtatggat
     8941 attcacacgc tcaatggtct cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac
     9001 cagccactgg ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat tacggggagc
     9061 aaaggttatc tgtttcatgg cgcggtcctg atacaaaacg ggtccagcca tcatacttca
     9121 caaggacttc actttcagac aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat
     9181 ggaacatttc gacgtggcga ttattggcct cggcccggca gggtcggcgt tggcacgaaa
     9241 gttagccggc aaaatgcagg tgatcgcgct ggataaaaag caccagcatg gtactgaagg
     9301 tttcagcaaa ccttgtggcg gtctgctggc accggacgcg cagcgatctt ttattcgcga
     9361 tgggctgacg cttcctgtcg atgtgatcgc taatccgcag attttcagcg tcaaaaccgt
     9421 cgacgtcgcc gcatcgctca cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca
     9481 tgctttcgac ttgtggatga aatcgctgat ccccgccagc gttgaggttt atcacgacag
     9541 cctgtgtcga aaaatctggc gtgaggatga taaatggcat gtcatttttc gtgcagacgg
     9601 ctgggagcag catattaccg cccgctatct ggtcggtgcc gatggtgcca actcgatggt
     9661 gcggcgacat ctctacccgg atcatcaaat ccgtaaatat gtcgcaatcc agcagtggtt
     9721 cgcagagaaa catccggtgc cgttctactc ctgcatcttt gataatgcga taactgactg
     9781 ttattcatgg agtatcagca aagacggtta ttttatcttt ggcggtgcct atccaatgaa
     9841 agacggtcag acgcgtttca cgactctgaa agagaaaatg agcgcctttc agttccagtt
     9901 tggcaaagcg gtgaaaagcg aaaaatgcac ggtgctgttt ccctcacgct ggcaggattt
     9961 tgtctgcggt aaggacaacg cctttctgat tggtgaagcg gcgggattta tcagcgccag
    10021 ctcgctggag gggattagct atgcgctgga tagcgcagac attctgcgtt cggtgttact
    10081 gaagcagcca gagaagctca ataccgctta ctggcgcgcc acccgcaaac tgcgtttaaa
    10141 actcttcggc aagatagtaa aaagccgatg cctgaccgca ccggctttaa gaaagtggat
    10201 tatgcgcagt ggtgtggcac atattccaca gttgaaggat tagccaacgc gcttcacatc
    10261 gcccaccagc aggatataag agagcgcgcc aatcaacgcg acggcggaga tataaaccag
    10321 tgcaggtgcg aaaccgtaac cctgcgccag gtaccccacc accagcggaa cggtgatgcc
    10381 gcccagacca ccggcgaagt taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc
    10441 cagagaagaa accagcgacc aggtaattga agcaaaaccg ttaccgaaga atgccagcgc
    10501 catcaggcac ataatcatca tcggatcgtt agtgtagtta gcgcccataa tgcaggtgga
    10561 gatcagcaag ccgcagataa tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg
    10621 taccagcaga tccgctaccc agccagagag caggacgccg acaaacgccg cgaggaatgg
    10681 caccgtggtc ataaagcctg ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt
    10741 cgggaaccag gttaagaaaa accacagtgt agaagccacc gcaaattgcc caagatagac
    10801 gccgatcagt ttacgatgga acaccagttt ccagtctttg gctgttaacg gctgacgtgc
    10861 ctctttcttc actggcgcat caccatccac cagaccgccg ccatcacgaa tgtaatccag
    10921 ttcagctttg ctgatgcctt tggtcaggcg cggcggctga taaaccttaa accaaatcag
    10981 cgaccagata atgccgatac caccagtcac aatgaacacc cagtgccagc tcaacatctc
    11041 ctgaatccag atcagcagcg gagtcaaaaa cgccagaccg acaaactgac cagacgtata
    11101 aaaaccaacg gcggaggcgc gttcatgttc cgggaaccag ctggtcacca tccggttatt
    11161 ggtcgggaaa gcgggcgctt cgaaaatacc ggttatcgca cgcagaccaa ttaatgacat
    11221 taatcccgtg gcaaagccct ggaataaagt cgccactgac cagccaaata tcgcaataaa
    11281 ataagtcacg cgagaaccta cgcgatctaa aaaccaaccg ccggggatct ggcatagcgt
    11341 ataaagccag gcgaaggccg aaaatacata gcccatttcc gctttcgtaa tgccgaactc
    11401 ttcctgaata tgggcggaag ccacggccag gttagcgcgg tcaacataac aaatgaccac
    11461 cgtaataaag atcatcacca gcgtcagata acgccgacgc cccggctttg ctgcattaac
    11521 gggaatatcc atagcgagct ttctccagat tttgggcata gcgaagccgc tcaccatgcc
    11581 ctgtaattta cagagggtta tatttttgta ttgctgtttt agtgcccgat gaggggctta
    11641 cgtggcagga attaccactc tgctacgctg ttatcttcat gacgccagag tggattacgc
    11701 cagtccgggg catttttact gaactcaata actttagctt cgtcgatttc cacgcctaag
    11761 cccggtttcg ttagcggttt aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc
    11821 acaaagtcga gtaactccgc gcctttgttg taatgaattc ccatactttg ttcctgaagt
    11881 accgcgttat aggaaacaaa gtcgatatgc agacaagccg ccagtgcaat cggtccgagc
    11941 ggacagtgcg gcgcaagggt cacgtcatag gcttctgcca ttccggcaat tttgtagcat
    12001 tcggtaatac cgcctgcatg ggagagatcc ggttgtagaa tcgaaatacc gcctgcctcc
    12061 agcacgcgtt taaaatcgaa acgtgagaac atgcgctcac ccgccgccag tggaatatgc
    12121 gtttgtgccg ccaatttcgg gtagtattcg gcttgttccg ccagcaccgg ctcttcaata
    12181 aacagcgggc gatacggctc cagttcttta atcagcactt tcgccattgg cgcgctgacg
    12241 cgaccgtgga aatcaagacc aaactcaatc tgattgccaa aagcttcacg aatttgtgcc
    12301 acggtgttga ctgccgcatc taccgcgcgg gagttatcaa ttagccccag ttcttcacaa
    12361 ccgttcagtt tgaaggtatc gaagccgatt tcgcgcaggg ttttaatacc gtcgatcaca
    12421 tccgccggac gatcgccgcc gacccaactg taggctttaa ttttgtcgcg aaccaggccg
    12481 cccatcagtt gccagaccga cgcattcagc actttacctt tgatatccca taacgcctgg
    12541 tcgatcccgg caatggcgct catcaggatt ggaccgccac gataaaatcc ggcgcgatac
    12601 atcacttgcc ataagtcatt gatgcgctat ggatcctgac caatcaaata gtcacccagc
    12661 tcgtgaactg ccgcttccac cgtgcgggcg cggccttcaa tcactggctc gccccagccg
    12721 acgacgcctt catcggtttc aattttcagg aacatccagc ggggaggtaa acgatacgtg
    12781 gtaattttgg tgattttcat tgcactgcct ctcgatacgc cttaacaaat gctgctgcct
    12841 gctgcgcggt gcgttctacg gactgcccgg cgcgatagag atcgctgcct aagcccgccc
    12901 cagcacaacc tgcgtctatc cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa
    12961 agactgcgat gtccgatggc aataccgctt ttaacgcttt gatgtattgc ggaccaaaag
    13021 ccgatgacgg aaatattttc agcgcctgcg cgcccgcttc gagcgcggta aaggcttcgg
    13081 tcgccgtcgc gcagccgggg cagacggtca tgccgtagcc caccgcacgg cggatcactt
    13141 cactatggat attgggcgta acgatgagct gacagcccat cctggcgagc gcatcgacct
    13201 gttcaggttt cagtaccgta cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga
    13261 tggcgggaat gctttgctcc cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc
    13321 cggcgtcaat caccgcgcca acatgcgcca gcgcctcgtc gggcttaatg ccgcgcaaaa
    13381 tggcgatcag cggaagttta gtttgccact gcatgagcga tgctccttat accagcctga
    13441 aatgccgtgt cgcccgccac cgccgtcacg tcgcaaccca tcgcctgaaa ggcttgctgg
    13501 tagcgcgcgg tcaacgatgt tccggcgaca agggtgatgg cgtgttgatg ggccacatag
    13561 tcgcgcatac tggcgacctc tgcgccaatc aacaaaccag agagaaattc actgacctgt
    13621 tcacggggaa gtgttcccag cacatgcgag gcacgaactt caaaaagctg cggcaatatg
    13681 gcgggcgtat taagaccacg ctcaaggcca gccgtgaagg catcggcaga gttttcctgc
    13741 ggcggcaaac ctgcaccaat caatgagtga tttaacagta aatgatgtaa ttcaccggtc
    13801 atcacggtgc gaaaatcgtt gatttgctgg ctatcggcct gcacccattt gcaatgcgta
    13861 ccgggcatga cataaagaga ggaaggagcc agagctcgcg cgccgatcaa ttgagtttct
    13921 tcgccgcgca tcacattgtg gttatcatca tgagagacgc ataatccggg aataatccag
    13981 atattgtcgc caacagacgt taattgttcg ccaatagacg aaaaatgggc aggaacagat
    14041 aaatacggag caactttcca gccgacattg ctgccgacca ttcctgccat taccactggc
    14101 gtattctctt cacgccagtc ggtcgtgact tctgctaaca ccgcagccgg agattttccg
    14161 ttcaggcgcg tgacgcctgc ttctgattgc ctgctctcca ggcagtggtc gccctgataa
    14221 agccaggcgc gcagattggt cgatccccag tcaattgcga tgtagcgagc tgtcatgtga
    14281 tttcctttaa ccttcgtgtc gagctggcga tcatggtaag cgccgcctgc tctgccgcat
    14341 cgccgtcctg atgccgtatc gcatcgaaca gcgccttatg ttcctggagc gtttgcggca
    14401 tgttggcctc atcgcccatc caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg
    14461 caatgctaag ttgctgtaac accgggttat gcaccgactg cagcaccgcc tcgtggtagc
    14521 gaatatccgc ttcgttaaac gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg
    14581 ccgattcaat ctgcgccaga tcgctggagg tcgcgcgctc tgctgcccaa cgggcaatcg
    14641 ccggttccac cagatttcgc acttcgctca tggcactgat aagccgtggg tcgtagtcgt
    14701 tttccagcac ccattgcagt acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca
    14761 caaacgcgcc gcgataacgt ttcatttcaa tcagccgttt cgccatcaac gaacggaaca
    14821 cctcacggat gatgttgcgc gaggttgcaa actcctcaca gagttccgcc tcagccggaa
    14881 gcggcgagcc tggtacgtat ttgccgtgaa cgatctgttt acccagcgta atgacgatgc
    14941 gatcggtttt attgagagtc atggagagtc cttgtgctct gtatgttctt ctctacttta
    15001 ccccgatcga tgcataatca ggaaagtatc aatcgcagtc agacaccatc acggggctat
    15061 cgcaggcaaa ggatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt
    15121 accttatcac agatggtgcc gatgacatcg ttaagatgct caaggatcca gctcttaacc
    15181 ggcacaatat tcaggttgct gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat
    15241 ttaccggcac aatatcactc tattacaact ggagcacaga agaagaaaaa gcacgagtga
    15301 cacaatatcg ttttgcttgt ggtgttcagc actgtacccg gacagtagaa aacctggaag
    15361 gtactatcca tgagaaaaat aaaaacatgg attactcaaa cgtaatggct ttctaccatc
    15421 catttaaagt gcgattttat gaataccatt cacccagagg cattccggat ggtgtttccg
    15481 cagcattact gccagtgact gttacgctgg acatcattac tgcaccgctg caatttctgg
    15541 ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg
    15601 cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggtta
    15661 gatttggtga caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca
    15721 ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt
    15781 tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg
    15841 cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc
    15901 acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc aatcatcatc
    15961 actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaata
    16021 gtggcaacga aagattcatg cactgtgtag tagctgatat cgcggttggc ggtgtacagc
    16081 gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa
    16141 cgatagtcgt catagctgag agcagtttgc gccacggtgc taccatcagc tgccttctgt
    16201 actagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc
    16261 aggtagttgt gcacacctgc atacgggcga cccgtcgtta gcacgacatt cacgccacgg
    16321 gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga
    16381 agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttcccc aggtagattg
    16441 gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag
    16501 ggggatgcaa cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc
    16561 agttccatac ctgcggaaag cagatcgtta ttggcttgtg gtgacacttc accttgcggt
    16621 gagagcgcat caataatctt cggcaattgc tctgccagta aactggaagc tgtactggta
    16681 tccacgccaa gtttttgccc gagatcggac accgcatttg tgccgagcgc cgattccagt
    16741 tgctcgccac taaccgattg attgccctgt tgattactca gccaggttga gagaatggcc
    16801 cctaagccgc cactttgcag tttttccagc agcacctgaa tgccgccctg ctcctcaacc
    16861 caacttaaaa tagcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca
    16921 tcaaaaagcc ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat
    16981 ttgcgggatg ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt
    17041 tcttatagct tcttgccgga tgcggcgtga acgccttatc cggcctacaa aatcatgaaa
    17101 attcaataca ttgcaagatt ttcgtaggcc tgataagcgt agcgcatcag gcacgctcgc
    17161 atggttagcg ccattaaata tcgatattcg ccgctttcag ggcgttctct tcaataaacg
    17221 cacggcgcgg ttcaacggcg tcgcccatca gcgtggtgaa caactggtcg gcagcaatcg
    17281 catctttaac ggtaacgcgc agcatacgac ggctttccgg gtccatggtg gtttcccaca
    17341 gctgttccgg gttcatctcg cccagacctt tataacgctg gatggagagg ccgcgacggg
    17401 actctttcac cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc
    17461 cacgttcgat aaacgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga
    17521 tacgacgata ttcgccaccg gtgataaact cgtgatccag cggatagtca gtatccacac
    17581 cgtgggtacg cacgcgaaca atcggttcaa acaggttttg ttcggcattg gtgtggacat
    17641 caaacttcca ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca
    17701 cccagcgggt aacggtctgc tcatcagaga ggtcggcttc cgtcagcgtc ggctgataga
    17761 taagctcttt cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg
    17821 tcgcgttgta ctcagacacc aatttctcta acgcttcgcc agccaatgcc ggtgcgctgg
    17881 cgttggtgtg cagcgttgcg ccatccagcg cgatagagat ctggtactga tccatcgctt
    17941 cgtcgtcttt aatgtactgt tcttgcttgc ctttcttcac tttgtacagc ggcggctgag
    18001 cgatgtagac gtgaccgcgt tcaacgattt ccggcatctg acgatagaaa aaggtcaaca
    18061 gcagcgtacg aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat
    18121 aacgcagttt gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa
    18181 gcgtcgccac ttcctgagaa gagagcatct tatcgaagcg cgctttctca acgttgagga
    18241 ttttaccctt cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc
    18301 cgcccgcgga gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc
    18361 agtctgccag tttgcccggc aggcccgcta agtcgagcgc acctttacgg cgggtcattt
    18421 cacgcgcgcg acgcgccgct tcacgggcac gggcagcatc gataattttg ccaaccacga
    18481 ttttcgcgtc ggttgggttt tccagcaggt attctgccag cagttcgttc atctgctgtt
    18541 caaccgctga tttcacctca gaagaaacca gtttgtcttt ggtctgggag gagaatttcg
    18601 ggtccggcac tttcacggaa acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg
    18661 cgctaacttt ggcttttttg ctgtagcctt ctttgtccat gtaggcgttc agggtacggg
    18721 tcatcgccgc acggaagcct gccaggtgag taccgccgtc acgctgcgga atgttgttgg
    18781 taaagcagta gatgttttcc tggaagccat cgttccactg caacgccact tcgacgccaa
    18841 taccgtcttt ttcagtggag aagtagaaga tattcgggtg gatcggcgtt ttgttcttgt
    18901 tcagatattc aacgaacgcc ttgatgccgc cttcatagtg gaagtggtct tctttgccgt
    18961 cgcgcttgtc gcgcagacga atggaaacgc cggagttgag gaacgacaac tcacgcagac
    19021 gtttcgccag aatttcatat tcgaactcgg tcacattggt gaaggtttcg aggctgggcc
    19081 agaaacgcac catggtgccg gttttttcag tctcgccggt aaccgccaac ggtgcctgcg
    19141 gtacaccgtg ttcgtagatc tgacggtgaa ttttaccctc gcgctggata accagctcca
    19201 gtttttgcga cagggcgttt actaccgaaa caccaacgcc gtgcagaccg ccggacactt
    19261 tataggagtt atcgtcaaat ttaccgcctg cgtgcagaac ggtcatgatc acttccgccg
    19321 ccgatacgcc ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag
    19381 agacagagtt atcggcgtga atggtgacga taatttcttt acagtgaccc gcgagcgctt
    19441 cgtcgatagc gttatctacc acctcgaata ccatgtggtg cagaccggtg ccgtcatccg
    19501 tgtcgccgat atacataccc gggcgcttac gcaccgcatc cagccctttc aggactttga
    19561 tactggagga gtcataagaa ttcgacatca acgtttctcg ctcatttata cttgggttaa
    19621 tccgttattt tacccttttc cacggtaaac atcttcgaat tttcgtccga catgtctata
    19681 acgtgttcag cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt taagcggctg
    19741 gcaagcagcc cgcgacgctc atcatcaagc tcagaggcaa aatcatctat caggtagaga
    19801 caccgccgcc cgctttcacg ggtgaggaac tctccttgcg ccagacgtaa ggcgcacatc
    19861 aacagcttaa gctgcccacg cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg
    19921 cgtaagtccg ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa
    19981 ttacgttcca gcacctcagc atattctgtc tctttctccc agccgcgctg gaaagagaaa
    20041 gtcagagaaa actcagggag aaattgctta caagtatcgg ccatatcagc cgcgataccg
    20101 gcgctatact ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag ctctttatcc
    20161 cacgggcgta gctgttcgta acgtgtcacc tggcgcagcg ccgcattgcg ctgcttgagc
    20221 aatcgcttga gattgctcca ggcggtgaaa aatccgggtt cgttgtgaaa gcatccccag
    20281 tcgaggaatg ctcttctgta tttggggccg ccgttgagta aagtaaaccc ttctggcgtt
    20341 atcaactgca ttggcatcag gtgcgccagt tccgcgacct tatgcccgtc tgtaccgtcg
    20401 atgcggactt tgctgtcgcc ctgtttgtct ttggttaagc caatcgctgt ctcgcgctct
    20461 tcgccctgta atcgcccgtg gagaacaaac gcctcctgct catggcgaat gacgcgacca
    20521 atctgcaaac tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc cagcacgctg
    20581 gttttgccac tgccgttggc acctaccaga aagttaaagc cgggagataa agcgagatcc
    20641 gcggtttcaa tgttgcggaa atcgcggatc aacaagcggg tgagggacat tacagtctca
    20701 ttggcatgac aacataggca gccgactgtg atgcggcatc ttcaatctgc acgctggaaa
    20761 ccgaatcggt cagcatcatg cggacgtttt cacatttcag cgcgttcaga acatccagca
    20821 cgtagctgac gttgaagccg atttccatct ccgcaccgct ataggtaacg tcgaggatct
    20881 cttccgcttc ttcctgttcc gggttgttgg cggtgatttt cagctggttt tcgctgacat
    20941 aaagacgtac gccgcggaat ttctcgttag agagaatcgc cgcgcgagca aacgcctgct
    21001 tgagcagatc gcagccagct tccagatgtt tgtccgggtt cttcggcaga acgcggcgat
    21061 aatccgggaa gcgaccatcc accagtttgg aggtgaagat aaagtcgcca acgtgggcgc
    21121 gaatgttgtt gctgccaatc tgtacgcgca gcggattgtc gccgccgtcg agcatacgca
    21181 tcagttcaat cacgccttta cgcggcacga tcaccgaatg gcttggcaaa gattgaccaa
    21241 ttggcattga acagactgcc agacggtggc cgtcggtcgc cacggtgcgc agttcttcgc
    21301 cttcggtttc aaacagcata ccgtttaagt aatagcgaac gtcctgatgc gccatagaaa
    21361 actgggtcgc ttcaatcaga cgcttcatcg tcgcctgcgg cagggtaaat tcgacttcgc
    21421 tctgccagtc atcgaggttc gggaaatccg ccgctggcag ggtagacagc gaaaaacggc
    21481 tacgcccgga gcgcaccagc atccgttcac cttccagctg tacggcaatt tccgcgcctt
    21541 caggcagacc acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct
    21601 cgtgtggctg aaccagcgca acgcgtgcca ccatttccat ctcgagatca gtaccggtca
    21661 gcgacaacgt accgtcagca acctgtaaca gcagattacc gagaatcggt agcgtaggac
    21721 gaccacctaa cggcccgctc acctgttgta gcggttttaa taaatgctca cgttctacgg
    21781 taaatttcat aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata
    21841 tcgtggctct cttcacgcaa ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg
    21901 tcacgaccac caaacgcatc gccaatctcc ggcagactgt ggttagtcag ctctttcgcc
    21961 agcgccatcg ccatctggcg cggacgcgcc accgagcggg atcgacgctt ggaaaggaga
    22021 tccgcgactt tgatcttgta gtactccgcc accgtcttct gaatattgtc gatggtgacc
    22081 agtttttcct gcaatgccag caagtcgcgc agtgcctcac gcacgaagtc gatggtgatc
    22141 gcccgtccgg taaagttggc gttagcaata acgcggttca gcgccccttc cagctcacgt
    22201 acgttagatc gtagacgctt ggcgataaag aacgccactt cgcctggcaa acgaatgtca
    22261 ttttcgtcgg cctttttcat caggatcgcc acgcgggttt ccagctctgg cggttcgatc
    22321 gccacagtca gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct
    22381 ttcggatagc gatccgaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag
    22441 gtgtggaaaa actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc
    22501 agcagtgcat ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg
    22561 gctttaacca tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc
    22621 ttgcgcgcca taatgccgtt acccaccgca tgcagcaggt gagttttacc cagacccgtg
    22681 ccgccataaa ggaacaatgg gttataggca ccgccagggt tatccgccac ctggcgagcc
    22741 gccgcgcgcg ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg
    22801 tttacgttag aacgataggt cggttctgcc ggggccggga cgttatccca acctgagcgc
    22861 gtagaaggcg cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg
    22921 ctcgtcactg ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc
    22981 tgtggggcat ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg
    23041 tcccgtaccc aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc
    23101 agttccgcct gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc
    23161 aatcgggcaa gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa
    23221 gtcgataatg actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc
    23281 ttgagggtga cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac
    23341 gatcgggacc gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt
    23401 tcccgattta tccacaggac tttccagacc tcgctaagtg taaacgatcc tgccgcgagg
    23461 cgggcacgat ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa
    23521 tcgatctaat aaagatctag gacgatcctt gcgctttacc catcagcccg tataatcctc
    23581 cacccggcgc gccatgctgg ttttcactgg tgtgaggtcg tacattttcc ctgcgaaaag
    23641 gtgcggaaaa gcgcggtaaa taaggaaaga gaattgactc cggagtgtac aattattaca
    23701 atccggcctc tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat
    23761 ccaataaagc cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc
    23821 aaccgtctgt actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa
    23881 atggtcgtca ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta
    23941 agtaataaag ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta
    24001 actcccagtc aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt
    24061 accattctcg gccgcctgaa ttcgctgggg catccccgta tcggtcttac agtcgccaag
    24121 aaaaacgttc gacgcgccca tgaacgcaat cggattaaac gtctgacgcg tgaaagcttc
    24181 cgtctgcgcc aacatgaact cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt
    24241 gccgacctcg ataaccgtgc tctctcggaa gcgttggaaa aattatggcg ccgccactgt
    24301 cgcctggctc gcgggtcctg atagccctca ttcgggtcta tcaacgcctg attagtccgc
    24361 tactcgggcc gcattgtcgt ttcactccaa cctgttcaag ctacggaatt gaggcattgc
    24421 gcaggtttgg agtgataaaa ggcagttggt tgacggtgaa acgcgtatta aaatgccacc
    24481 ctttacaccc tggtggtgac gatcccgtcc cgcccggacc atttgatacc agagaacact
    24541 aacgatggat tcgcaacgca atcttttagt catcgctttg ctgttcgtgt ctttcatgat
    24601 ctggcaagcc tgggagcagg ataaaaaccc gcaacctcag gcccaacaga ccacgcagac
    24661 aacgaccacc gcagcgggta gcgccgccga ccagggcgta ccggccagtg gccaggggaa
    24721 actgatctcg gttaagaccg acgtgcttga tctgaccatc aacacccgtg gtggtgatgt
    24781 tgagcaagct ctgctgcctg cttacccgaa agagctgaac tctacccagc cgttccagct
    24841 gctggaaact tcaccgcagt ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg
    24901 cccggataac ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt
    24961 gctggctgaa ggtcaaaacg aactgcaggt gccgatgacg tataccgacg cggcaggcaa
    25021 cacgtttacc aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg tcaactacaa
    25081 cgtgcagaac gctggcgaga aaccgctgga aatctccacc tttggtcagt tgaagcaatc
    25141 catcactctg ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc acaccttccg
    25201 cggcgcggcg tactccacgc ctgacgagaa gtacgagaaa tacaagttcg ataccattgc
    25261 cgataacgaa aacctgaaca tctcttcgaa aggtggttgg gtggcaatgc tgcaacagta
    25321 tttcgcgacg gcgtggatcc cgcataacga cggtaccaac aacttctata ccgctaatct
    25381 gggtaacggc atcgccgcta tcggctataa atctcagccg gtactggttc agcctggtca
    25441 gactggcgcg atgaacagca ccctgtgggt tggcccggaa atccaggaca aaatggcagc
    25501 tgttgctccg cacctggatc tgaccgttga ttacggttgg ttgtggttca tctctcagcc
    25561 gctgttcaaa ctgctgaaat ggatccatag ctttgtgggt aactggggct tctccattat
    25621 catcatcacc tttatcgttc gtggcatcat gtacccgctg accaaagcgc agtacacctc
    25681 catggcgaag atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc gtctgggcga
    25741 tgacaaacag cgtatcagcc aggaaatgat ggcgctgtac aaagctgaga aggttaaccc
    25801 gctgggcggc tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt tgtactacat
    25861 gctgatgggt tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc
    25921 ggcacaggac ccgtactaca tcctgccgat cctgatgggc gtaacgatgt tcttcattca
    25981 gaagatgtcg ccgaccacag tgaccgaccc gatgcagcag aagatcatga cctttatgcc
    26041 ggtcatcttc accgtgttct tcctgtggtt cccgtcaggt ctggtgctgt actatatcgt
    26101 cagcaacctg gtaaccatta ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg
    26161 cctgcatagc cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa aataagggcg
    26221 gtcagttgac cgcctttttt cttttcgtag ggtggataag caccgcgcat ccgccacaca
    26281 aagcaacagg aacatcatga gcgataatga cactatcgta gcccaggcca cgcctccggg
    26341 acgtggcggc gttggcatcc tgcgcatctc cggcctaaaa gcccgtgaag ttgccgaaac
    26401 cgtgctgggt aaactgccta agccgcgcta cgccgattat cttccgttta aagacgccga
    26461 cggcagcgtg ctcgatcagg ggattgcgct atggttccct ggcccgaact cgttcaccgg
    26521 cgaagatgtg ctggaactgc agggtcatgg cggtccggtg atcctcgacc tgctgttaaa
    26581 acgcattctg accattcccg gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc
    26641 gtttcttaac gataaactcg acttagccca ggccgaggcg attgccgatc ttatcgacgc
    26701 cagttcggaa caggcggccc gttcggcact taactcgctg caaggcgcat tctccgcacg
    26761 ggtcaaccat ctggtggaag ccctcaccca cttgcgcatt tacgtcgaag cggcaattga
    26821 tttccctgat gaagagatcg atttcctctc cgacggcaaa attgaagccc agcttaatga
    26881 cgtgattgcc gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga
    26941 agggatgaag gtggtgattg ccggacgtcc taacgccggt aaatcgagcc tgttaaacgc
    27001 gctggcgggc cgtgaagcag caatcgtaac tgatatcgcc ggaaccacac gtgacgtgct
    27061 gcgtgagcat atccacattg acggaatgcc gctgcatatc atcgataccg ctgggctacg
    27121 tgaagccagt gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca
    27181 ggccgaccgc gtgctgttta tggtcgatgg caccacaaca gacgccgtgg atccggcaga
    27241 gatctggccg gaatttatcg cccgtctgcc agcgaaacta ccgatcaccg tggtgcgcaa
    27301 taaagccgat atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat
    27361 tcgtctctcg gcaaggacag gtgaaggcgt ggaggtgctg cgtaaccatc tcaaacagag
    27421 catgggcttt gacaccaaca tggaaggcgg cttcctggcg cgtcgtcgcc acctacaggc
    27481 gctggaacag gcagcggagc atttgcagca aggtaaagcg caactgttag gtgcctgggc
    27541 gggtgaactg ctggcggaag agttgcgcct ggcacagcag aacttaagcg aaatcaccgg
    27601 ggaatttacc tctgatgatt tgctggggcg gattttctcc agcttctgta ttggtaagta
    27661 accgcgctta cgaagccgta ttctgactgt cagatgcggc ttcgcttcat tgttagcact
    27721 cctgttattc ctcaaccctt tttttaaaca ttaaaattct tacgtaattt ataatcttta
    27781 aaaaaagcat ttaatattgc tccc
//
LOCUS       DAZOZD010000031        27671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOZD010000031 DAZOZD010000000
VERSION     DAZOZD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.031"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            14..676
                     /gene="acrS"
                     /locus_tag="ACHUPB_001075"
     CDS             14..676
                     /gene="acrS"
                     /locus_tag="ACHUPB_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR0757957.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(679..858)
                     /locus_tag="ACHUPB_001076"
     CDS             complement(679..858)
                     /locus_tag="ACHUPB_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0757958.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(942..1826)
                     /gene="yhdJ"
                     /locus_tag="ACHUPB_001077"
     CDS             complement(942..1826)
                     /gene="yhdJ"
                     /locus_tag="ACHUPB_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0757959.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1912..2208)
                     /gene="fis"
                     /locus_tag="ACHUPB_001078"
     CDS             complement(1912..2208)
                     /gene="fis"
                     /locus_tag="ACHUPB_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0757960.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2234..3199)
                     /gene="dusB"
                     /locus_tag="ACHUPB_001079"
     CDS             complement(2234..3199)
                     /gene="dusB"
                     /locus_tag="ACHUPB_001079"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0757961.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3528..4409)
                     /gene="prmA"
                     /locus_tag="ACHUPB_001080"
     CDS             complement(3528..4409)
                     /gene="prmA"
                     /locus_tag="ACHUPB_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0757962.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4421..5872)
                     /gene="panF"
                     /locus_tag="ACHUPB_001081"
     CDS             complement(4421..5872)
                     /gene="panF"
                     /locus_tag="ACHUPB_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0757963.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5862..6104)
                     /locus_tag="ACHUPB_001082"
     CDS             complement(5862..6104)
                     /locus_tag="ACHUPB_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0757964.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6213..7562)
                     /gene="accC"
                     /locus_tag="ACHUPB_001083"
     CDS             complement(6213..7562)
                     /gene="accC"
                     /locus_tag="ACHUPB_001083"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0757965.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7573..8043)
                     /gene="accB"
                     /locus_tag="ACHUPB_001084"
     CDS             complement(7573..8043)
                     /gene="accB"
                     /locus_tag="ACHUPB_001084"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0757966.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9021..9995)
                     /locus_tag="ACHUPB_001085"
     CDS             complement(9021..9995)
                     /locus_tag="ACHUPB_001085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0757967.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10147..12087
                     /gene="csrD"
                     /locus_tag="ACHUPB_001086"
     CDS             10147..12087
                     /gene="csrD"
                     /locus_tag="ACHUPB_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0757968.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12392..13435
                     /gene="mreB"
                     /locus_tag="ACHUPB_001087"
     CDS             12392..13435
                     /gene="mreB"
                     /locus_tag="ACHUPB_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0757969.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13501..14604
                     /gene="mreC"
                     /locus_tag="ACHUPB_001088"
     CDS             13501..14604
                     /gene="mreC"
                     /locus_tag="ACHUPB_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0757970.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14604..15092
                     /gene="mreD"
                     /locus_tag="ACHUPB_001089"
     CDS             14604..15092
                     /gene="mreD"
                     /locus_tag="ACHUPB_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0757971.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15101..15694
                     /gene="yhdE"
                     /locus_tag="ACHUPB_001090"
     CDS             15101..15694
                     /gene="yhdE"
                     /locus_tag="ACHUPB_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0757972.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15684..17153
                     /gene="rng"
                     /locus_tag="ACHUPB_001091"
     CDS             15684..17153
                     /gene="rng"
                     /locus_tag="ACHUPB_001091"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0757973.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17221..21021
                     /gene="yhdP"
                     /locus_tag="ACHUPB_001092"
     CDS             17221..21021
                     /gene="yhdP"
                     /locus_tag="ACHUPB_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0757974.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21177..22622
                     /gene="tldD"
                     /locus_tag="ACHUPB_001093"
     CDS             21177..22622
                     /gene="tldD"
                     /locus_tag="ACHUPB_001093"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0757975.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22750..23615)
                     /locus_tag="ACHUPB_001094"
                     /pseudo
     CDS             complement(22750..23615)
                     /locus_tag="ACHUPB_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            23798..24001
                     /gene="aaeX"
                     /locus_tag="ACHUPB_001095"
     CDS             23798..24001
                     /gene="aaeX"
                     /locus_tag="ACHUPB_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0757976.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24009..24941
                     /gene="aaeA"
                     /locus_tag="ACHUPB_001096"
     CDS             24009..24941
                     /gene="aaeA"
                     /locus_tag="ACHUPB_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0757977.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24947..26914
                     /gene="aaeB"
                     /locus_tag="ACHUPB_001097"
     CDS             24947..26914
                     /gene="aaeB"
                     /locus_tag="ACHUPB_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0757978.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27006..27278
                     /locus_tag="ACHUPB_001098"
     CDS             27006..27278
                     /locus_tag="ACHUPB_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0757979.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27334..27597)
                     /gene="yhcN"
                     /locus_tag="ACHUPB_001099"
     CDS             complement(27334..27597)
                     /gene="yhcN"
                     /locus_tag="ACHUPB_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0757980.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6643 a         6930 c         7358 g         6740 t
ORIGIN      
        1 tgaataatta atcatggcaa aaagaaccaa agccgaagct ctgaagaccc ggcaagaact
       61 gattgaaact gccatcgccc agtttgcgca acatggcgta agcaagacga cgttcaacga
      121 cattgccgat gccgctaacg ttacgcgtgg cgctatctac tggcacttcg agaacaagac
      181 ccaactgttt aatgagatgt ggttgcaaca gccttcattg cgggagttaa tccaggaaca
      241 cttgacggct ggattagagc atgacccgtt tcaacaattg cgtgaaaaat tgattgtcgg
      301 cttgcaatat attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct tatatcacaa
      361 atgtgaattt aatgatggga tgctggccga gggagtgata cgcgaaaaga tgggctttaa
      421 tccgcagact ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg tagcaaataa
      481 cctcgattta gatgttgtga tgattattat tgatggtgcc ttcagcggaa ttgttcaaaa
      541 ctggttaatg aatatggcgg gttatgatct ttataaacaa gcacccgcgc tggtcgataa
      601 cgtattaaga atgttcatgc cagatgaaaa cataacgaaa ttaattcatc aaacaaatga
      661 attaagtgtc atgtaaattc acattctgtc ctgaaaattc agttcgtcac agttaaccag
      721 ttttagtgga taaactgagt tcatattacg gcatttgtct gtttctgttg tcagcagttc
      781 aatccactgc atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa cgacgagcca
      841 ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg gcaggaaata
      901 tacacgaaat gaataatctg aaaaccaggc ttacacttcc tttactttgc aatgagatcg
      961 gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct ttttttcact
     1021 ttcgccagtt cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag ccccattttg
     1081 atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat ggctacggca
     1141 ccggtagtaa agctaccggc aaacgggtcg agaacgatat cgcctgggtt ggaagaggcg
     1201 agaataatgc gtttcagtaa ggcttccggt ttttgcgtcg ggtggttctc atattcatcc
     1261 atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg atgattgtat
     1321 ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt tttggcttct
     1381 accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat catcaggatg
     1441 ggttcgtaca tggagccgta gtgttttttc gcctgtactc cagaactgtc atatgaccag
     1501 acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa gggcatgttt
     1561 tccgtactgt tcataatgta catgctgccc tgctttttca gaacgcggtg gcactctaca
     1621 atcacttcaa acagccagtc gataaacaga tcttctttcc aggcttcgat cagaccatca
     1681 aaatttttac cgatgttata cggtgggtcg gcaaagatca gatcgacgct ttcagtaggt
     1741 agctttttaa gttcggcaag ggcatcaccg tgaataatgg tcttagcttc attaccaaac
     1801 cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat aaaaaaggcg
     1861 cttccccatg ccgagtagcg cctttttaat caagcattta gctaacctga attagttcat
     1921 gccgtatttt ttcaattttt tacgcagcgt accacggttg atgcccatca tcagcgcagc
     1981 acgggtctgg ttaccacggg tgtattgcat caccatgtcc aacaggggct gttctacttc
     2041 agccagtacc agctcataga ggtcattcac atcctgacca ttcagttgag caaaatagtt
     2101 cttcagtgcc tgtttaaccg agtcacgcag gggtttttgg gttacctgat cctgagagtt
     2161 aacggtagaa acggtcagta cgtcagaatt tacgcgttgt tcgaacatag ttctgtcagc
     2221 tctttatttc tgtttacgca aaattttcga agtatgcctc caacgcctcc agctgttcgc
     2281 tggcatcctc aatggcgttg aatgtgcgcc gaaactggtc atttggagcg tgttcctgga
     2341 gataccagga aacgtgttta cgtgcaattc ggtacccttt tgccggacca taaaagtcat
     2401 gcagttcccg aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc agcgggggca
     2461 gcaactcccc agtgtccaga taatgctgga tttcccgaaa gatccagggt cttccctgag
     2521 ctgcgcggcc tatcatcagg gcatccgccc ctgtatagtc gagcacagct ctggctttaa
     2581 gcgggtcagt aatgtcgcca ttcgcgataa ccggaatgga aactttctgc ttaactgccc
     2641 gaatactgtc gtactcagct tctccattga acaaacaggc gcgtgtacgg ccatgaatgg
     2701 tcagagcctg aatgccacag tcttcagcca gttgggcaat ctcttcgcag ttacggtgtt
     2761 ccggtgccca gccggtgcga atcttcaggg taacaggaac gtccactgca ttgacgacct
     2821 cggtaaggat cgatttaacg acatccgggt actgcaagag ggctgaacct gcgagcttgc
     2881 gattcacttt tttagccggg caacccatat tgatatcaat aatctgggca ccgctttcca
     2941 cgttaatacg tgctgcatct gccatttctt tcggatcgct accagcaatt tgcacggtgc
     3001 gaataccggg ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt tcccaaacct
     3061 gtgggttaga agacatcatc tcggatactg tcaatccggc tcccatctcg tagcacaacg
     3121 tccgaaaagg tctgtctgta atgccagcca tgggcgctgc gatcaggcga tttctgagct
     3181 gatattgtcc gatgcgcatg agttaagaaa tgaccatact gtgactgcaa ggcggcgtat
     3241 attacgcatt ttttgcacga gatgaaaggc caaactttga ccaatcctct gagatggatc
     3301 aaagaattgc atttaaaatg agcgtggtga gataattact cataaaaatc atcatattag
     3361 aaaatagtga ctaaaattta cactcaaaga aatttgagta agttctcaat ttttctttat
     3421 gaatgaaaat tttggcacgc aaatttgcgt aaataaccgg caaatttacg tgccttttgt
     3481 gagcttgctc gcacttcgcc ccgcgtcacc ctacggcgat gcgaaggtta attcttacga
     3541 ccggtaatgc ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa gctgtcgaca
     3601 taagcttcac aaacgctctc tgcctggctt gccagaatac cggaaaggcc cagcaaaccg
     3661 cctgaaaccg gcaggacgct gattaacggt gccagttcac gtaatgggcc tgcaaggatg
     3721 ttagcgacca ccacgtcggc tttcatttct tctggctgat ctttcggtaa gtagagttcc
     3781 agacggtcag aaacgccatt acgttcggcg ttgtcgcggc tagcctgaat cgcctgcgga
     3841 tcgatatcaa taccaatggc ttttgctgca cccagtttca gcgccgcgat agccagaatg
     3901 ccggaaccac agccaaagtc gatgactatt ttaccggtta aatcgaggct gtcgagccac
     3961 tgcaggcaca gagaggtagt tggatgggta cccgtaccaa acgccagccc tggatctaac
     4021 atcacgttga cggcgttttc gtccggcaca tcacgccagc tagggcagat ccacagtcgt
     4081 tcaccaaagc gcatcgggtg gaaattatcc atccattcgc gctcccagtc tttatcttcc
     4141 agttgttcga ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc
     4201 accacgtcgt tcatatcggt ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac
     4261 aggcgcgttt cgcccggcag cggctcaaat actggcgtat cgtgggtatc ctgaaaagtg
     4321 atagaaacgg cacccgcttc catcagcgca tcgctaagat cttcagcgtt cgcgccggtg
     4381 gtgttcagtt tcagttggat ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa
     4441 cggtagcttg cgggacggat gtaccgaaac ggtttccgac caggaaagcc agcaaactta
     4501 gtagtaacga gggcacgata gggtggaagc ccaggtactg aatattcagc gtcgcgagta
     4561 cggcatacag cacgccgcca acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt
     4621 cccagtaaag acccagcacc agcggccaca ggaaaacggc ttccagccca ccgaaggcca
     4681 gcaaattcag ccagatgatc atttctggcg gcttccaggc ggcaagcagc agcaacgcgc
     4741 cgagaactaa cgtaattacc gccgacatcc gcttcagacg cgtctcgttt tgcatttgat
     4801 ccggacggat attcagatag agatctttaa tgatcgtagc ggaactttgt agcaattggg
     4861 cgttaattgt cgacatgatc gcagccatag gtgcagccag aaagatcccg gcagcaaacg
     4921 gtggcagcac ttttaccatt aacgtcggga tcaccagatc cggtacggtg agatcgggga
     4981 tcactgcacg acctaacgct ccggctaggt gcataccgaa catcaggatt gcgactacaa
     5041 tcgtaccgat gatgatcccc cggtgtaccg ctttgctgtc tttataagag atacagcgca
     5101 ccgcagtatg cggcaggcca atcacgccaa agcacaccag tacccagaac gacgtcataa
     5161 aggcaggcga cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt
     5221 gcaaggtctg tactgcgttg cttaagccgc cagcggcatg taccacgcca ataagcagca
     5281 caacggtgcc aatcagcatc acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa
     5341 agccgccaaa ggcggtatac aacgcaatgc tgataccgaa aatcagcagc ccggtttcat
     5401 aaggaatacc cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc acggtcattg
     5461 ccccaacgaa cgcaaccagc aaactcaaac tcgccagcca caccagaaga cgactctggt
     5521 agcgggcaaa cagcatgtcg ttcagcgtca ctgcattgta gcggcgcgca agaatcgcaa
     5581 acttcttgcc gagaataccg agtgaaagcc agactgcagg aagctgaatc atcgccagca
     5641 atacccagcc cagcccgtat ttataagcag ctcctggccc gccgataaac gaactggcac
     5701 tgatataggt cgcggtgagc gtcatcgcca gcacaatacc gcccatagag cggctgccga
     5761 ggaaatactc attaaggaag gtgccggtgc tccgtttacg catcgcataa accgagatac
     5821 cgaacaccac caccagatag gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc
     5881 tccagtggga tatcgcgata gataaatttc accatcgccc agcacagtcc aataaacagt
     5941 agcggcgtca ggatgcaggc catctcaaac cagcgcggaa agccggtaaa accgggggca
     6001 acgccaggta agtaagcggc tactaaccaa actgccagat acaaaagggt cagccccagc
     6061 gcccagcgcg cctctttatg ggcctgaaca aaacgagtgt ccattttttg tccctgatgg
     6121 gtgaagaaag cgaggattgt accttatggg ggttgtcgat ccccagtaat aaaaaaggcc
     6181 ggaaaatccg gccttttggc gcttttgcag tcttattttt cctgaagacc gagttttttc
     6241 tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat gatgcggatc
     6301 tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc attcttcatg
     6361 cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat cattgagtca
     6421 tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac gccaaaaccg
     6481 ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt cggatcttcg
     6541 gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac
     6601 agcggttgac cggcagcgat acggagctgt tctttgatca ggtcaacgcc agtgatcatt
     6661 tcggtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata gaactcgccg
     6721 ttttcgaaca gaaactcgaa agtacctgca ccgcgatagc cgatatcaac acacgcttta
     6781 gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc tggcgcttct
     6841 tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag atagatagcg
     6901 ttgccccgac cgtcagccag tacctgaatc tcgacgtggc gaggattttc caggtatttc
     6961 tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt catggagatg
     7021 gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccacc gccgccgccg
     7081 gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcacggtt tttatccata
     7141 tcgtcgccca acgggccgtc agaacccggt acgcaaggga cgcccgcttt tttcatcgcc
     7201 gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg gccgatgaag
     7261 ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag gaagccgtaa
     7321 cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc cgggatgttc
     7381 agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc cagtaatacg
     7441 tgttttagat cgcgatccgc gctggagtgc acagcgacag tcttgatgcc cagttcttta
     7501 caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat tttatccagc
     7561 atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg gttgtccact
     7621 ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt tcatcatttt
     7681 catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca cttcgatgaa
     7741 cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg aacgtacgat
     7801 gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc
     7861 tgcgttagat tgagctggct gctgcatcat tggtgcagcg taagcttgtt gcatcacagg
     7921 gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt cagaaatttc
     7981 cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct tacgaatatc
     8041 catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc accgccgtct
     8101 gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg ccgggacgcc
     8161 ggtctatttt gccccagagt atcaatgtta gacttgacgg acattgtgca gcgatctgct
     8221 gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag
     8281 cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt agcaattggc
     8341 agctaaatac tggtctcatc agggaagata atcaacagct aacatgtaaa taaccttcaa
     8401 caccgtgtaa tttgcaacaa gccgcacaat tcacgaaatt agcgccatca tcgacggaac
     8461 ttcttataac gtaaggtgaa aagctgtaaa gccagccctg cgaagatgag gggctgcaat
     8521 gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac aagatagacg
     8581 acattacgca gcggttgctg gggattaccc aattttcact gcatcgtctg gatagatgtg
     8641 aatgctaaag cacgcaaaat caaccgactg acaattctca gcattaaata ggttcgagtg
     8701 gttaatttgt agtcaaataa aatcagatta ttcaccccat ggtaccagaa atcagtaaca
     8761 ggttaagcgt agtgtcgccc aggcaaagca ccataaacct aacaggcgac ggaggcgtat
     8821 caataatggc tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt cgaaatcatg
     8881 actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac gaaagctgtc
     8941 cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag ttccctctct
     9001 tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg gatctggtta
     9061 ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt tgccgcctgg
     9121 gtatagaatg attccggtaa atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct
     9181 ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat aaatggcatg
     9241 accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca gccgccgtaa
     9301 ttcatttgcg ccagcacttt tgccagcact ttgtcgccaa cagtgtcaat tgccccagcc
     9361 cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag aatacggctg
     9421 gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc aacgacctga
     9481 taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact ggcacccgtc
     9541 acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat cacacacagc
     9601 atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag cccttgcggc
     9661 atggcaacca gccagtcacc tttcactcgc gcctgctccg ccagcccacc ccagtggttt
     9721 tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg cggatcttcg
     9781 ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt acggatgatt
     9841 tttcccttac cggtaatcgc cagcgcatct ttatagttca ggctcgacca gtgaacatcg
     9901 accgtgacat cgccctccgg caggcgactt tcgtccagag tctgtactga tgcgagagtt
     9961 ttgccgtcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga
    10021 ttggaaaata atttctgaag attatactcg ctaatggaaa agcaaagccg atgaagcgca
    10081 ataaattgcg agataaatct gatttgctag tatgcccgct tcctcactat cggagttaac
    10141 acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac cgggttaaca
    10201 atttttgtga ctttgctggg ctgttcgcta agtttctaca acgcaattca gtataagttt
    10261 agtcatcgcg ttcaggcggt ggcgacggcg attgataccc accttgtgtc gaatgacttc
    10321 agcacattaa ggccacaaat taccgaatta atgatgtcgg cagatatcgt tcgtgtagac
    10381 ctgctccatg gtgataaaca ggtttatacc ctggccagaa atggtagtta tcgtccggtt
    10441 ggctccagcg atctgtttcg cgaactgagt gttccgttga taaagcatcc ggggatgtcg
    10501 ctgcgtctgg tttatcagga tccgatgggc aactatttcc attctttaat gactaccgcg
    10561 ccgctcacgg gggcgattgg ctttatcatt gttatgctct tcctggcggt acgctggtta
    10621 caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat cttaaacggt
    10681 gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac cagcagtgcg
    10741 ctagatacgc tgcttcgtga aattcagaac gcacgcgaac aacacagccg tcttgatacg
    10801 ctgatccgct cttatgccgc ccaggacgtg aaaaccggcc tcaataaccg actctttttc
    10861 gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca cgggatcgtg
    10921 atgatgattc gtctgccgga tttcaatatg ttgagcgaca cttgggggca cagccaggtt
    10981 gaagaacagt tcttcactct gactaatctg ctgtcgacat ttatgatgcg ctaccctggc
    11041 gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc agttaccgca ccggacgtta
    11101 aaagaggcag agagcatcgc cggtcagtta atcaaagccg tcgatacctt gccgaacaat
    11161 aaaatgctcg atcgcgacga tatgatccac attggtatct gcgcctggcg tagtggtcag
    11221 gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg attgcagggc
    11281 ggcaatagct gggctattta cgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc
    11341 tggcgtacgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg
    11401 gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat cttcgatggt
    11461 aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg cttatcggaa
    11521 gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg gccagaggaa
    11581 aatctggcga ttcaggttac cgttgagtcg ctgattcgcc cgcgttttca gcgttggctg
    11641 cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat tgaacttgca
    11701 gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt agtgaatgct
    11761 ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac cagttggatc
    11821 aaagaactta atgttgagtt actcaagctc catccggggc tggtcagaaa cattgagaag
    11881 cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg gaccagcacc
    11941 cacgtttacg ccaccggcgt gcgttcgcga agcgagtggc agaccctgat tcagcgcggt
    12001 gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac taacgtgaaa
    12061 aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac gtagaataac
    12121 gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc
    12181 agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt
    12241 tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt tcaccgtagc
    12301 agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg gattttcttt
    12361 tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg gcatgttttc
    12421 caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa aaggacaagg
    12481 catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa
    12541 aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat
    12601 tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat
    12661 gctccagcac ttcatcaaac aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt
    12721 tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc
    12781 gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg ccgcaattgg
    12841 tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg gtggtggtac
    12901 cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg tgcgcattgg
    12961 tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat
    13021 cggtgaagcc accgcagaac gtatcaagca cgaaatcggt tcggcttacc cgggcgatga
    13081 agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac gcggttttac
    13141 cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc
    13201 ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct ccgagcgcgg
    13261 catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt taatggaaga
    13321 aaccggcatt ccagtagttg ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg
    13381 caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag agtaatcgga
    13441 tgcaggcagg ggaaatgtct gtttaccctg cctggtctga tacgagaata cgcataactt
    13501 atgaagccaa tttttagccg tggcccgtcg ctacagattc gccttattct ggcggtgctg
    13561 gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca aatccgtact
    13621 tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg tgaattgctg
    13681 gatggcgtat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa ccgggcgtta
    13741 cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata caaacaggag
    13801 aacgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg
    13861 actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat cgataaaggt
    13921 agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt tgttggtcag
    13981 gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg
    14041 ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa cggttgtacg
    14101 gatgatttgc agcttgagca tctgccagcg aatacggata ttcgtgttgg tgatgtgctg
    14161 gtgacctccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc
    14221 gtaaaactcg atacccagcg cgcttatact gtgattcagg cgcgtccgac tgcaggactg
    14281 caacgtttgc gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc taacccgatg
    14341 acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat gccgcaggta
    14401 ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg gatcgctcag
    14461 ccgactccgc agcaaccgac gacaggaaat gcagctactg cgcctgctgc gcccacacag
    14521 cctgctgcta atcgctctcc acaaagggct acgccgccgc aaagtggtgc tcaaccgcct
    14581 gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgt tgggtaatct
    14641 ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat aacctgattg
    14701 ttttccggcc aaactgggtg ttactcatct tgttgtattg gatcctggcc ttgcctcatc
    14761 gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc agcggctcga
    14821 cgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg ctgaaatacc
    14881 agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg
    14941 tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct ttcagaccgg
    15001 aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc ttgctgatgc
    15061 gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc tgtatttagc
    15121 ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggtgtgacct ttgaacgtat
    15181 tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg
    15241 tctggcgcgc gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc tcccggtgct
    15301 gggtgcggat actatcgtta tcctgaatgg agaagtgctg gagaaaccgc gcgacgcaga
    15361 gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga tgacggcagt
    15421 ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg tgactttcag
    15481 aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc
    15541 aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa atggcagcta
    15601 tcacgccgta gtcggcttac ctctggttga aacgtatgaa ttattaagta attttaacgc
    15661 actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta acgccttcgg
    15721 aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt gaacgtgagg
    15781 cgcgacgcgg aatagtaggc aatatctaca agggtcgtgt aagtcgtgta cttccgggta
    15841 tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat gcatccgata
    15901 tcatgccgca caccgaatgt gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca
    15961 tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg
    16021 gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat ctggtcttta
    16081 tgccaggggc ttctcacgtt ggggtttccc aacgtattga aagcgaatca gaacgtgaac
    16141 gcctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc atccgtaccg
    16201 cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg aaacgcgtct
    16261 ggaccaaagt tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg tacggcgaac
    16321 tggcgctggc tcagcgtgtt ctgcgtgatt tcgccgacgc cgaactggac cgcattcgcg
    16381 ttgactcacg cctgacttac gaagcattac ttgagttcac ctcggagtac attcccgaga
    16441 tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc tttgatgtcg
    16501 aaaacgaaat ccagcgagcg ctggaacgca aagtagaact gaaatccggt ggttatctca
    16561 ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg tttgtcggtc
    16621 atcgcaatct ggacgacacc attttcaata ccaatattga agcgacgcag gctatcgctc
    16681 gccagttacg gttgcgtaat ctgggcggga ttatcattat tgatttcatc gatatgaata
    16741 atgaagatca ccgccgccgc gtgctgcact cgctggagca ggcgttgagc aaagaccggg
    16801 tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg cgtaaacgca
    16861 cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac ggtcgcggaa
    16921 cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt cgtgtccacc
    16981 atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc gaagccttga
    17041 aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa caggttaaag
    17101 tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg taaacagatg
    17161 ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa ggagtgacgg
    17221 gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt gatcgctgcc
    17281 ctgctggtta gcggcctgcg tattgcttta ccgcatcttg acgcctggcg tccggaaatc
    17341 ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct ctcagccagc
    17401 tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact aaaagatggc
    17461 ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag cctgttacat
    17521 atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac caacactcct
    17581 atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga tctgtttctt
    17641 cgtcaatttg accatttcga tctccgcgac agtgaagtca gtttcctgac gccatccggt
    17701 cagcgtgccg agctggcgat cccacaactc acctggctga acgatccacg tcgacaccgt
    17761 gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat gcaggtgcgc
    17821 atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct acaggcggat
    17881 gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc tctggaaacg
    17941 gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac cggcggtgac
    18001 gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg
    18061 gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt ctctattccc
    18121 gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac gctggcctgg
    18181 ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact ccggattcgc
    18241 gccagtaatc tggagctggc aggcctggag ggcatacgcc cgctggccgc gaaactttca
    18301 cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa cactctggcg
    18361 ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg gagcgatctg
    18421 gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac gctttccggc
    18481 agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg
    18541 gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg gctgaacaat
    18601 gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc cgtccatgcg
    18661 cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg tattctggct
    18721 ggcatcagta ccgatgatgg ttcacaagcc tggcactatt tcccggaaaa cttgatgggt
    18781 aaagacctgg ttgattactt aagtggcgcg attcagggcg gtgaagcgga taacgcgacg
    18841 ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg tcagtttgaa
    18901 gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc tgcattaact
    18961 aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac cgatggcgtt
    19021 aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta ctcgaaagaa
    19081 aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc ttactttgat
    19141 gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct cgacggcgat
    19201 gtgaatgctc gcttacatct tgatatcccg ctgaacggcg agctggtaac cgcgaaaggt
    19261 gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcac cctgaaaaat
    19321 ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact gacagcaagc
    19381 tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa agcctaccag
    19441 gttgcggtga atctcaacgg taactggcaa ccggcgaaaa ccggcgttct gcctgaagcg
    19501 gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat tgatctgcct
    19561 tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa tgtgagtagt
    19621 cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa cgttaaggtt
    19681 gatggcaatc tcaacagctt tgaattaacc ggacaggctg gtgcggataa ccatttcaat
    19741 agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc ggcagacagt
    19801 aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac tcaatatgcc gccgatgaat
    19861 ggtgccgagt ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg tggtgcagcg
    19921 agtttcccac aatacataac gttacgtacg cctatgttgt cactgggaaa tcagcaatgg
    19981 aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga ggcgcaaggg
    20041 cgtgaaatca acgccacgct agcgatgcgt aataacgcgc cgtggctggc gaatatcaaa
    20101 tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct
    20161 ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat acgatgcaca
    20221 gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatatcac catttctggc
    20281 gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct tactgccgat
    20341 ggtgaatggg ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa actgcgcggg
    20401 cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgcccatacg ccagtcgtca
    20461 tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga tgaagcgacg
    20521 ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat caataccgga
    20581 catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt
    20641 gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag caccgcgtgg
    20701 attaaagacg gggttatgca caccgacgac acgctggtgg atggcctgga ggcggatatc
    20761 gccatgaaag ggtcggtaaa tctggtacgt cgcgacctga atatggaagc ggttgtcgca
    20821 ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc cattgttggc
    20881 gcggcagttt ttgccgctag taaagtgctg gggccgctgt ggagcaaagt ctccattttg
    20941 cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg
    21001 cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc tcatatgata
    21061 atcgttgcca aaggccaacg agccagaaca taaccgtagg ccggataggg cgttcatgcc
    21121 gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca aaaacgatga
    21181 gtctgaacct ggtaagtgaa caattgctag cggcgaacag cctgaaacat caggacttgt
    21241 tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc tattttcagt
    21301 cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt tcttacaaca
    21361 tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt gcttacgctg
    21421 accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc atcgtccgtg
    21481 atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg ttgtatacct
    21541 cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg
    21601 ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc ctcagtggtg
    21661 tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat gtccgtccgc
    21721 tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa cgcggtgcca
    21781 gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac ggcgaagtcc
    21841 gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt tctgccgttg
    21901 ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc
    21961 atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgcggcact tcagtattta
    22021 gtgggcaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat gacggcacga
    22081 tggtcgatcg ccgtggttcg gtggcgattg atgacgaagg tacgccaggc cagtacaacg
    22141 tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac gcgcgtttga
    22201 tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg cccatgccgc
    22261 gtatgactaa cacctatatg ctgccgggta aatcgacccc gcaggaaatt attgaatccg
    22321 ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc acctccggca
    22381 aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta acgaagccgg
    22441 tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt tcgatggttg
    22501 gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc
    22561 cggttggcgt gggccagcca acgctgaaag ttgataacct gactgttggc ggtactgcgt
    22621 aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa cggtcccctc
    22681 gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtagaa cgtaacagta
    22741 caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt taccgacctc
    22801 aacaaaataa tccgtcagcg agttgatcac gacctgtacc ttcagcggca gcttatcttt
    22861 ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca gcaggatctc
    22921 cagctcaccg cgattgatct cgttgatcac ccacatcagc ggcacgtagg cgatcccggc
    22981 acccgccgtc agccagcgca ccagcgtcat cggatcatta gtcacaaatc ttccttgtgg
    23041 gatcaggcga gtcgagatcc cttccggtgc gatcagttca aattcattgt cgggccgcac
    23101 gctgtattca agccatgaat gactactcaa atcggcgggt ttttccggta tgccgtattg
    23161 tgtgagatag cttttcgcgg cgcacaccac catcggcatc gcgcccagac ggcgggaaaa
    23221 caggctggaa ttccggtaac cagattgacg ctcaaacctg ggtattcttt cagcattttt
    23281 gctgtcagcc cggcgagaac attttgtgcc atagttgaag aacagccaat gcgcagcgtc
    23341 ccgatggggg tgttattgaa ggcatacagt tgctcatgaa catcctgcac ttcatgaagc
    23401 atccgacggc agccctggta gtaaattctg ccggcttcgg tcaggccaat gctgcgtgtg
    23461 ctacggttta acagctttac ctgcaactca tcttccagtt ttgacaccgt ctgactgatg
    23521 gacgaaacgc tcatctgtag ctgtctggcg gcggcggtaa aagagccaaa ttcaactact
    23581 ttggcaaaca ccgacatgcg ttttagtcgt tccattattc actctgactt aaaagtgatt
    23641 tagatcacat aatatagata acagcataac agttacgcta atatattaaa tatcaatcta
    23701 cagcaatgtt gctctcgccc ggctttccat gcccttctct gcggcgacag atgctgaaaa
    23761 taataacgcc tgctctctct ttacaaccaa ggtcaacatg agtctgtttc ccgttatcgt
    23821 ggtgtttgga ctgtccttcc caccgatatt ttttgaattg cttttatcac tggcgatttt
    23881 ctggctggtg cgccgggtac ttgtgccaac aggtatctac gactttgtct ggcatccggc
    23941 gttgttcaac accgcgctct attgctgctt gttttatttg atatcgcgac tgttcgtttg
    24001 aggttgaagt gaaaacacta ataagaaaat tctcccgtac ggccatcacg gtcgtattag
    24061 tcattctagc cttcatcgca atttttaatg cctgggtcta ttacaccgaa tccccctgga
    24121 cgcgtgacgc gcgctttagc gctgacgtcg ttgcgatcgc gccggacgtt tctggactca
    24181 ttacccaggt gaatgttcat gataaccagc tggtgaaaaa aggacagata ctgttcacca
    24241 tcgaccagcc gcgctatcaa aaggcgcttg aggaagcgca agccgatgtt gcttattatc
    24301 aggtactggc acaggagaaa cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg
    24361 cgatgtctcg cgaagagatc gaccaggcca acaacgtact acaaacggtt ctgcatcagt
    24421 tagcgaaagc gcaggcgacc cgcgatctgg caaaactgga tcttgaacgc acggtgatcc
    24481 gcgcgccagc agatggctgg gtgaccaacc tcaacgtcta taccggtgag tttattactc
    24541 gaggatcaac ggcggttgcg ctggtgaaac agaactcctt ctatgtactg gcctatatgg
    24601 aagaaactaa gctggaaggg gggcgtccgg ggtatcgtgc agagatcacg ccgcttggca
    24661 gtaacaaagt gctgaaaggg actgttgata gtgttgccgc aggggtcacc aacgccagca
    24721 gcacgcgtga cgacaaaggg atggcgacta tagactctaa tcttgaatgg gtgcgtcttg
    24781 cgcaacgtgt tccggttcgt attcgtctcg acaaccagca agagaacatc tggcctgcgg
    24841 gcaccactgc tacagtggtg gtcactggca aacaagatcg cgacgaaagc caggattcgt
    24901 tcttccgtaa aatggcccat cgcctgcgtg agtttggtta atcacgatgg gtattttctc
    24961 cattgctaac caacatattc gctttgcggt aaaactggcg accgccattg tactggcgct
    25021 gtttgttggc tttcacttcc agctggaaac gccacgctgg gcggtactga cagcggcgat
    25081 tgttgccgcc ggtccggcct ttgctgcggg aggtgaaccg tattctggcg ctattcgcta
    25141 tcgtggcttt ttgcgcatca tcggcacatt tattggctgt attgccggac tggtgatcat
    25201 cattgcgatg atccgcgcac cattattgat gattctggtg tgctgtatct gggccggttt
    25261 ttgtacctgg atatcctcgc tggtacgaat agaaaactcg tacgcgtggg ggctggccgg
    25321 ttataccgcg ctgatcattg tgatcaccat tcagccggaa ccattgctta cgccgcagtt
    25381 tgccgtcgaa cgttgtagcg agatcgttat cggtattgtg tgtgcgatta tggcggattt
    25441 gctcttttct ccgcgatcga tcaaacaaga agtagatcga gagctggaaa gtttgctggt
    25501 cgcgcaatat caattaatgc aactctgtat caagcatggc gatggtgaag ttgtcgataa
    25561 agcctggggc gacctggtgc gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa
    25621 tatggaatct tcccgctggg cgcgggccaa tcgacgttta aaagcgatca atacgctatc
    25681 gctgacgctg attacccaat cctgcgaaac ttatcttatt cagaatacgc gcccggaatt
    25741 gatcactgat actttccgcg aattttttga cacgccggta gaaaccgcgc aggacgtcca
    25801 caagcagctc aaacgcctgc ggagagttat cgcctggacc ggggaacggg aaacgcctgt
    25861 caccatttat agctgggtcg cggcggcaac gcgttatcag cttctcaagc gcggcgttat
    25921 cagtaacaca aaaatcaacg ccaccgaaga agagatcctg caaggcgaac cggaagtaaa
    25981 agtagagtca gccgaacgtc atcatgcaat ggttaacttc tggcgaacca cactttcctg
    26041 cattctgggc acgcttttct ggctgtggac gggctggact tccggcagtg gtgcaatggt
    26101 gatgattgcg gtcgtgacgt cactggcaat gcgtttgccg aatccacgca tggtggcgat
    26161 cgactttatc tatgggacgc tggccgcgct gccgttaggg ctgctctact ttttggtgat
    26221 tatccctaat acccaacaga gcatgttgct gctgtgtatt agcctggcag tgctgggatt
    26281 cttcctcggt atagaagtac agaaacggcg actgggctcg atgggggcac tggccagcac
    26341 cataaatatt atcgtgctgg ataacccgat gactttccat ttcagtcagt ttctcgacag
    26401 cgcattaggg caaatcgtcg gctgtgtgct cgcgttcacc gttattttgc tggtgcggga
    26461 taaatcgcgc gacaggactg gacgtgtact gcttaatcag tttgtttttg ccgctgtttc
    26521 cgcgatgact accaatgtgg cacgtcgtaa agagaaccac ctcccggcac tttatcagca
    26581 gctgtttttg ctgatgaata agttcccagg ggatttgccg aaatttcgcc tggcgctgac
    26641 gatgattatc gcgcaccagc gcctgcgtga tgcgccgatc ccggttaacg aggatttatc
    26701 ggcgtttcac cgacaaatgc gccgcacagc agaccatgtg atatctgccc gtagcgatga
    26761 taaacgtcgt cggtactttg gccagttgct ggaagaactg gaaatctacc aggaaaagct
    26821 acgcatctgg caagcgccac cgcaggtgac ggaaccggtt catcggctgg cggggatgct
    26881 ccataagtat caacatgcgt tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg
    26941 ttttttcatg gctatactta gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc
    27001 aacagatgaa tatttatacc tttgattttg atgagattga gagtcaggag gatttttatc
    27061 gtgactttag ccaaaccttt ggtctggcta aagataaggt acgcgatctc gactcactat
    27121 gggatgtgtt aatgaacgat gtcctgccgc taccacttga gattgaattt gttcatctgg
    27181 gagagaaaac gcgtcgccgt tttggcgcgt taatattgct gtttgatgag gcagaggaag
    27241 agctggaagg gcatttgcgt tttaatgttc gtcattagcg caaaaaaaag cccccgaacc
    27301 gggggcaata tcgtcggaca agacgatgag ggtttatttg tacagttcag ccgtagcgtg
    27361 ccaggtgtca ccgctacgag cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc
    27421 ttttttgttc agcatttcac gcatatccat tggcgaagac gccacaccac ttacggatac
    27481 ggtcccgatt gcttcacgat tttgtgcttg tgcagtatca atggagtcgg cagcgaatgc
    27541 accgaaagag agaacagaaa gtacgcttaa tgcagctaca gtggttttga ttttcatgat
    27601 ttttacctcg acataatctt ttagctgggc ctttgtttcg tgatccatat cacaaaatca
    27661 agcatacact a
//
LOCUS       DAZOZD010000032        27126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOZD010000032 DAZOZD010000000
VERSION     DAZOZD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.032"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHUPB_001100"
     CDS             <1..411
                     /locus_tag="ACHUPB_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0757981.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(459..1331)
                     /locus_tag="ACHUPB_001101"
     CDS             complement(459..1331)
                     /locus_tag="ACHUPB_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0757982.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(1343..2437)
                     /locus_tag="ACHUPB_001102"
     CDS             complement(1343..2437)
                     /locus_tag="ACHUPB_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0757983.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2470..3468)
                     /locus_tag="ACHUPB_001103"
     CDS             complement(2470..3468)
                     /locus_tag="ACHUPB_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0757984.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3493..5004)
                     /locus_tag="ACHUPB_001104"
     CDS             complement(3493..5004)
                     /locus_tag="ACHUPB_001104"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0757985.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(5027..6010)
                     /locus_tag="ACHUPB_001105"
     CDS             complement(5027..6010)
                     /locus_tag="ACHUPB_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR0757986.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(6107..9384)
                     /locus_tag="ACHUPB_001106"
                     /pseudo
     CDS             complement(6107..9384)
                     /locus_tag="ACHUPB_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            9502..10695
                     /locus_tag="ACHUPB_001107"
                     /pseudo
     CDS             9502..10695
                     /locus_tag="ACHUPB_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(10893..12146)
                     /gene="glyA"
                     /locus_tag="ACHUPB_001108"
     CDS             complement(10893..12146)
                     /gene="glyA"
                     /locus_tag="ACHUPB_001108"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0757987.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            12474..13664
                     /gene="hmpA"
                     /locus_tag="ACHUPB_001109"
     CDS             12474..13664
                     /gene="hmpA"
                     /locus_tag="ACHUPB_001109"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0757988.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13709..14047)
                     /gene="glnB"
                     /locus_tag="ACHUPB_001110"
     CDS             complement(13709..14047)
                     /gene="glnB"
                     /locus_tag="ACHUPB_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0757989.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14108..15442)
                     /gene="glrR"
                     /locus_tag="ACHUPB_001111"
     CDS             complement(14108..15442)
                     /gene="glrR"
                     /locus_tag="ACHUPB_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0757990.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(15432..16145)
                     /gene="qseG"
                     /locus_tag="ACHUPB_001112"
     CDS             complement(15432..16145)
                     /gene="qseG"
                     /locus_tag="ACHUPB_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0757991.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(16310..17737)
                     /gene="qseE"
                     /locus_tag="ACHUPB_001113"
     CDS             complement(16310..17737)
                     /gene="qseE"
                     /locus_tag="ACHUPB_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0757992.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(18295..22182)
                     /gene="purL"
                     /locus_tag="ACHUPB_001114"
                     /gene_synonym="purI"
     CDS             complement(18295..22182)
                     /gene="purL"
                     /locus_tag="ACHUPB_001114"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0757993.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            22440..23996
                     /gene="mltF"
                     /locus_tag="ACHUPB_001115"
     CDS             22440..23996
                     /gene="mltF"
                     /locus_tag="ACHUPB_001115"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0757994.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(23993..24496)
                     /gene="tadA"
                     /locus_tag="ACHUPB_001116"
     CDS             complement(23993..24496)
                     /gene="tadA"
                     /locus_tag="ACHUPB_001116"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0757995.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(24554..25189)
                     /gene="yfhb"
                     /locus_tag="ACHUPB_001117"
     CDS             complement(24554..25189)
                     /gene="yfhb"
                     /locus_tag="ACHUPB_001117"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0757996.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            25398..26246
                     /locus_tag="ACHUPB_001118"
     CDS             25398..26246
                     /locus_tag="ACHUPB_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0757997.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(26585..27126)
                     /locus_tag="ACHUPB_001119"
                     /pseudo
     CDS             complement(26585..27126)
                     /locus_tag="ACHUPB_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         6470 a         7462 c         6835 g         6359 t
ORIGIN      
        1 cgttattttg attttggagc tgcccgcccc gttttgttat taattgcccg tatcgccgtg
       61 gtcttaattt tcattatttt tggttttccc aaaatgatgg gctttgacgg tacggtccaa
      121 tatatggcct cgttgggcgc gccaatgccg atgctggcag cgattattgc ggtagttatg
      181 gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca cccgtccgct ggcggtgctg
      241 tttattttct acacgctggg tacggcggtg attggtcacc attactggga tatgaccggc
      301 gatgcggttg ggccaaatat gattaatttc tggaagaatg tcagtatcgc tggcgcgttc
      361 ttgctattgg caattaccgg gccgggggca atttctctcg atcggcgtta ggaaagatgc
      421 cggatgcggc gtgaacgcct tatccggcat ttaataaatt acagccactc tacccgcaac
      481 gacatctctg aagttgttga ttcccctggt gctagcgcaa tgagatcacc cccttccggg
      541 cgatgatgat catccggcgc atggctcatc ggttccagac agaagaaatc aaacgcatat
      601 cctttatcaa acgcagggtc ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt
      661 tccatgatga tggcataacc ttcctgcggc tgctcgatgc gggcctgacc attccatccg
      721 gcaaaaccat tgttcaccca ctggcgcggc aacggcgcga gctggttaaa atccagttcc
      781 tgcggtagct gctcgcaaaa ctcacccgcc agccactgct cccgttccag ccagtaaccg
      841 ctcgcctgcg cctgaatccg cgtttgtggt gacaacggaa aataaggatg ccagccggta
      901 ccaaatggca gcgtctctgc cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc
      961 gccgttaaat gaaacgcctg acttacccga tagtgataga caccgctgcg atgttcatac
     1021 accagacaca aactatcatc gctgtgcgag acacattgcc actcgcccag ccagccatcg
     1081 ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca gttgatactc acgcccctgc
     1141 cagacaaaac ggttgccgct cacccggttt gcaaatggca ccagcggaaa acatgaggca
     1201 tcggttgcca caccgctttt tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag
     1261 ccttcgatta cgccgccctg gtcagaaacg tccagcttta gcgacccgtg ggataaggta
     1321 tagatggtca tccgctaact ccttaatccg ggaagttaat cacaactttc ccgcatttgc
     1381 cgctcgccat cagcgcatag gcatcacctg cctgttccag cgagaagcga tgggtaatgg
     1441 cgttacgcgg ccacagcttc cagtcagtca aatcgtgggc gcatttttcc atatggaaca
     1501 gactggtcac ccaggagccg ataatccgcc gttgatggtg catcagatcg gcgctgacct
     1561 cgaattccac ttttccggtt tcaccaatgt aaaccacccg tccccagtca gcggtggatt
     1621 gcagtgccag caagcgacct gcggcattac cagaacaatc gagcgcaaca tccgcgccac
     1681 cgtgggtgag ttcggcgata atctgcggca gaccttcggt ggttgctaaa tagccgtgat
     1741 ccatcacccc taactgtttt gccatcgcca gacgttccgg cagcatatca acgccgatga
     1801 tccgttttgc accgcgacct ttcgccagca tcatcgccat catgccgacc ggccccagac
     1861 cgaccaccag cacgttatcg ctgccggaaa cttcgccgcg caaaatccct tcataggccg
     1921 tgccaacgcc gcaactgata aacgcaccat cttcgtagct cagcgcatcc ggcaggagga
     1981 tcagatcttt ttcttccgcc agcagatatt cggcatgacc gccgtcacgc tgccagccgt
     2041 aagccgcttt tcctttgcca gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa
     2101 aaccacagcc agaaatgtga tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc
     2161 cttgccccat cgccacaatc tgcccgcacg gttcatgacc gttgataaat ccctggtata
     2221 acggtttatc gggtgccgcc gccgtggcac ggtgttgatg atagatatag tggacatcgc
     2281 ttccgcaaat cccggaggat ttcattttga tcagtacctg gttaatcccc ggcgtcggca
     2341 ccgcaacttc ccgcagatcg acggttgaat ttcctggtaa ataagctgcc agcatcgttt
     2401 tcataaatcc ctcattaaca atacgattaa ttttcatccc tgccgcaccc gcgccagggg
     2461 cgttaaggtt tagcgtttcg ctttactgct tcgctgggtc agcaagatat tcgccagcac
     2521 cgccaccacg atgatgacgc cgcgtaccac ctgctggaaa aaggagttaa taccgagcag
     2581 caccagaccg ttaccgatta gcgtaatcac cagcacacca agcaatgtac cgaacaggga
     2641 accgcgaccg ccggaaagtg ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc
     2701 cagaccgttt gcggcacctg cgttaccgga accgaggcgt gccgccagca aaatgccggt
     2761 caccgccgct aataatcccg aaagggtaaa gataagaatg cgcacccgac gaacgttaat
     2821 accgcacaac tgcgccgccg tggcattacc gccaacggca aaaaccgagc gcccgaaggc
     2881 ggtttttcgg ctaatgaaca caaacagcgc aaacaacacc atcatgatca gcgcggatac
     2941 tggcacaccg agaaattgtc cgcccagcca gtccagcacc tcgttttcgt caatcggcac
     3001 tggcagtgcg ttggtcataa acagccccat tccgcgcagg gcgctccaca gccccagtgt
     3061 ggcaacgaaa cttggtacgt taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag
     3121 cgcgcctaac agcaacacca gcagacaagc aatcgccaac ggaacttcaa attgcagcaa
     3181 aaatgccagg cacaccgaga caaaagccac catcggccca acgctgacat caatttcacc
     3241 ggagataata atcagcgtca tcgcccaggc ggcaatccca atggtggcgg catcgcgcag
     3301 cacgttcatc tggttattca atgagataaa gccaggcgcg ttcagggaga agaccagata
     3361 aagaatggca atcaccacca gcaaaccgat ctcattaata tggcgactga caaattgttt
     3421 gagcgagacg ctcttgccct gcggcaatgg taatgacgaa gcagacatct tcaggttcct
     3481 cggtaaaatt catcagtgca cagacagaat ggcggacatc agctcatcca cattgaccgg
     3541 cgagtgaaac tcctgcgaga acgtgccgtg ctgtaataac aggatgcggt cacacaccag
     3601 cggcaactcc tccacttcac tggagataaa caccacgctt tttccttcgg cagccagctc
     3661 acggacaata cggtaaatct gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc
     3721 gagcaacaaa atctggctgg cggcatagac ccaacgaccg atcaccactt tttgctgatt
     3781 gccaccagaa agcgtgccga tgggcgtttc gctactagcg gccttgaccg tcatccgctg
     3841 catcacctct tcggtcaggc ggcggatggt ggaccattgc agcactccgt tggcgctgat
     3901 tttttgccga ttggtcagca ctgtattttc gtcaacgccc aaccagggaa tgatccccgc
     3961 ttctttgcgg ttttctggcg tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg
     4021 gcgcgtgatt ttctcgccgt tgataacaat ttcgccctgt tcatacgtct ccagcccaac
     4081 aatcgccttc agcaattcac tgcgccctgc ccccagcagg ccagcaatgc caagcacttc
     4141 gccgcgacgt aacgtaaagc tgatatcctc cagcttgggc ttatggcgta acgcacggac
     4201 ttccagtacg gcctgatcca caatttcctg aggggcaacc ggcgcaatat caacgtgatc
     4261 acgccctagc attagcgaca caatatgatg cgtggaggtg ttttcgagca tcacatcgcc
     4321 cgccacctga ccatcgcgca taacggtggc acaggaggca atgcggcgga tttcttccat
     4381 ccggtggctg acataaatca ccgccacgcc cagtgccgac atctttttca ccgcactgat
     4441 caccagttca acttctgcac tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg
     4501 cggctcgccc ttcatcaccc gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt
     4561 tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc
     4621 ctgcgccatt tgcaggtaat caatcatgcc gttgcggcga ggccattgac caaggcagag
     4681 gttttctgcc accgtcagcc cttccaccag acttaattcc tgataaaccg cacgaacccc
     4741 cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat
     4801 ccagatatca ccgctatccg ggcgttcgct gccggtaagc attcgaatga gagtcgattt
     4861 gcccgcgccg tttttaccta acagcgcacg aacttcgcct ttattgagcg tgaagttaac
     4921 gttatccagc gcaacgacgc cggggtaacg cttatttcct gccaccactt ttgctaccgg
     4981 gactgcctct gttgccgtga acatagggac ctctgcgaat cagcgattag ggcagaccat
     5041 caacgtgcgt tgccagccac tgtttaccat cttccgtttt ggtatagaga tcgattggca
     5101 cctgaatcac tttttcaccg tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa
     5161 cagcattgcc cattttctta ccggaaatat ccactaccgc tttcagcacc tgattgtttt
     5221 ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa aacagcaatt tttccggcct
     5281 gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc accgcctgac tcccccataa
     5341 tggcgttgag atccggcgtg gagataatca gtttttcacc aacggaaatc gctttattta
     5401 aaacagtccc ttcctgatta gcgacaattt gcgcgccggg aacgcgggat tttaatactt
     5461 cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa
     5521 ttttcggctg gtcaatttta ttggcaataa aataatcggc ggcagcgtta cccagttttt
     5581 taccaaattc cagtggatcg ccgaccagat acgccgagac atatttatcg acaccctttt
     5641 gattaataca ggtgttgtag caaatcaccg gaatacccgc ttcactggcg cgacgaacgg
     5701 tacggctact gccattttca gacactgccg ataaaataat ggcatcgaca ttacgcgcca
     5761 cgagggtatc aacaaaggta ctttctttcg aaatatcacc ctgggcgtta gtttcaatta
     5821 actgtacctg tactgaagaa tcttttgccg catcctgaac gccctgacgc actccagcgt
     5881 aatatcccag ggtatcaagg tatattgcac caatggtcat ttctttatcc gcagccctgg
     5941 caaatagtgc gctacctaat agcgtagcca ttaataatag attacgggtt gttctcattt
     6001 ttttaggcat agtgcctcct gtagagtttt tattaacaac ggcttattct aattattttg
     6061 tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa tagggtttag tgaacgtagt
     6121 taaaaataaa aggcatcacg gtggtgaata atgccgcttt cggccaggcc ggattaatcg
     6181 ccgtcaattc agcgcgagca gattcatatt gcgcaacctc cccgagtccc gcggacgcca
     6241 gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca tacagcgaca
     6301 acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat actggttttc gccatctctt
     6361 gcgcccactg tttcatttca ctaaacagtt gctgtgcgat ttgttgttcg cccagcagtc
     6421 gcagcgccat tccttgccag aagagataat caaccggctg atcgttgtaa taactgtgga
     6481 tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca acgcgtcgct tcagtttcat
     6541 cgccctgcgc gttggcgcat atcgcctgcc agaaccagat gtcgttatca gtttgccccg
     6601 gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc agttcgcagg
     6661 cctgctgcgg ctgtctggca tcaagatgct gccaggcgcg taataactgg ttgaggataa
     6721 actgactggt gaccttccct tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa
     6781 tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa attgagcagt tctgcggtca
     6841 tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt cgtttctccg
     6901 gtgtggctcc acttaactta tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg
     6961 gcgcaagctg ataagcatta tcaagataac gcgcggccag ttcataatcg tgttgcttat
     7021 tccacgcatg gatcgccaac ccgcgccagc catcggcaaa ctccggcgac atctctacgc
     7081 aacgttgcca taaagtaatg gctttgttgt agctgcgctt gttgtagtag aaacaggcca
     7141 gtaaatgacg ggcaaaccag cactcttcaa tactctccag cgccgccact tcttccagcg
     7201 tattcgggaa acggacaaac tgcgggaaga catcaatggc ttttgcgacc agttcgccgc
     7261 gttcggcttt cggcagcaag ctggcttgta gataaagcga taacgtgcgc tgacagtcca
     7321 gtgcgttcag catctctgtc gccagagtgg gcattcccca gttaatcagt tgcccggcgg
     7381 tcatcagggc gttaacgtcg cgtccctgac acagaccgcg ccactgcgcg agggctgatt
     7441 cgctacgacc atcgaaccag ttcagccacc acagaatggc gttcagcgga taatcacgca
     7501 gcagtttcgc gcgctgcaaa cgcgcgttgt cctgacgacc acttaacacc agcagcagat
     7561 tatgcaggca aagcacttcc tgattggttg ggcaggcgcg aagactttgt tggcaaaaat
     7621 ccagaccagc gtcgaagtta ccattacgcg ccgccagtcg tgccaggcca taatagccac
     7681 cggctttgct gttgccgctc cagaccgcac gccagaaatc ctcttcggct tgttgatatt
     7741 gtccctgacg ttcgtaagca ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt
     7801 tgttcagcgc atgtgcgcgt ttcagcgcct gactggcata cgccaccgct tgcgggaaat
     7861 ctgcgcggtt atattccagc atcgccagcg ccaggttaca gcgataatcc agcgggtcca
     7921 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
     7981 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gccagtggcg
     8041 ctttggcgac gtccggtaac ggcaacgctt gcggctgatg ttcctgataa ctcagtacaa
     8101 tattgccgtc ggcatcggaa agctcaatgg tcagcctatc aggattgata ccgtgcaaca
     8161 cgccctggat ggcggtcgca ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt
     8221 tgccgatttc gtggatcgcc aggcggtatc cgttcaacgg agagatggca tacagccccc
     8281 actcaatccc ctgctcacta cgctggagtt ttatcaccgc atcgcgggag gcattttgca
     8341 ccatgcccag agaatgataa ggcaggaaat actgctcaaa acgcttctct tcgtaagcat
     8401 caagccaggt aaaatcgggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
     8461 ggccgttatt gtcggtcaga ctcttatccc acgcctggcc aaattcactg tgtccccaac
     8521 tccactgttt tttacctggc gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt
     8581 catcgtgaca ccacgcgccg acaaaatcgt actgcgattt ttcggccata tatgaggttg
     8641 gaacgggcac atttttatag cgagaaatgt ccactccagc ggagtagtcc actttgtagt
     8701 aagtgccggt agcgatgggg aaagcggaga cggcccgttt gccgtgatca aacaccgcag
     8761 tcaaatccgg tgggaaaacg ctctgatgcc cttcaccccc tttcactgcc gggttggccc
     8821 accacaagaa atgacgcggc gtggcgttgc cgttatagac gcggctggcg atttccagcg
     8881 ccgcccaggg cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc
     8941 gcctacccac accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat
     9001 aaaggtggtc gggcgatgat gttgcggcca gttaaactca atcccaccgg aaatccacgg
     9061 ccccagcagc cccaccagcg caggtttaat gacttcattg tgataaacaa aatcgcgctg
     9121 tttcacttta tcccacgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt
     9181 gatgtagtcg ttttccagcc acaccgcctg ccaggatttc agggttttct gctcgctcag
     9241 cgtatcggtc acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc
     9301 caggaacatg ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc
     9361 ttgccacact tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa
     9421 agatttgtgg cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta
     9481 actaaacaag gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat
     9541 aacaaatgcg tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg
     9601 gcccggctgg cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc
     9661 gaaaaacggg tggtgaatat cgacgatgaa agccagacgc gcgggcatag tagcggtaca
     9721 tggctgattg cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt
     9781 gagtgtcagg ttgctaatgc ttgtttaagt ccgaaaggtg aatttgagta tttacagatt
     9841 gatgcaccga cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt
     9901 aaattgtggc cggaccgcac catcaatctg gcgctggcaa tccacggtca ggttgatccg
     9961 gtgaccggcg tgtcgcaaac catgccgcaa gcgccgtgga caacgccggt tgaggtgaag
    10021 tatctgctgg aagagaagct cggcattcgg gtgatggtcg ataatgactg cgtgatgctg
    10081 gcgctggcgg agaaatggca aaataattcg cagaaacggg atttctgcgt gatcaacgtt
    10141 gattacggca ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat
    10201 ggtagcggac agattagtca caccatcgtt aatccggatg gcgtcgtctg cgactgtgga
    10261 cgttacggct gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta
    10321 tggctaaaat cacaaccggt taatactcag cttgatccgg aaaaactgac tacagcgcag
    10381 ttaatcgctg cctggtagag tggagaaccg tggatcacca gttgggttga tcgcagtgcc
    10441 aatgccattg gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg
    10501 ttgtacggtc gcagttgtgc ctttggtgag cactggctta ataccattat tcgccagacg
    10561 ggatttaacc cgttcgaccg cgacgaaggg ccgagcgtga aagcaacgca aattggcttt
    10621 gggcaattaa gccgcgcaca acaggtgttg gggattggct atttgtatgt tgaggcgcag
    10681 ttacgacaga tttgatggcg caataacgta gaaaggcttc ccgaaggaag ccttgatgat
    10741 cacataccta agattgcctg atgcgctacg cttatcaggc ctacacggat attgcaatat
    10801 attgaatttg caaagttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    10861 acgagcacat tgacagcaaa tcaccgtttc gcttatgcgt aaaccgggaa acgtgcgcag
    10921 atgtcgagaa ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc
    10981 acgtcacaca tccagccagc cagttctttc gcttctgctt ctttgaagcc gcgacgcgta
    11041 atcgccggag tacccacgcg aataccggag gtcacaaacg ggctcttcgg atcgttcggt
    11101 acgctgtttt tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg
    11161 gtcaggtttt tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc
    11221 actttgtagc cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc
    11281 tgctggtaag ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg
    11341 attacgtgca tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg
    11401 tacagctctt cgctaccacc tttcgccagg atcaggccgc cgcgcggacc cgccagggtt
    11461 ttgtgagtgg tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca
    11521 gcaaccaggc ccgcaacgtg cgccatatca acgaacaggt aagcaccgat gctgtcagcg
    11581 atttcacgca ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc
    11641 attttcggct tgtgttcttt ggcttgtttt tccagatcgg cgtagtcgat atgaccggta
    11701 gcatcgatac cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg
    11761 tgagtcaggt gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc
    11821 agcgcggtgt agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag
    11881 tcagcgccga acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac
    11941 tcgcaaccgc cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga
    12001 gaaccctgcg cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg
    12061 tgctcttcct gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg
    12121 gcaatgttca tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt
    12181 tggcctttat aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga
    12241 taacaggtct tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg
    12301 gagaataaag agtttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt
    12361 cacttcatca cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt
    12421 ttataagatg catttgagat acatcaatta agatgcaaaa aaaggaagac catatgcttg
    12481 acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa
    12541 agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt
    12601 ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg
    12661 cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc
    12721 agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt
    12781 tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag
    12841 cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg
    12901 ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc
    12961 gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat
    13021 accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa
    13081 ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatt gcggtgaaac
    13141 gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg
    13201 tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga
    13261 cgttaatctc tgccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa
    13321 aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc gatgttcacg
    13381 cctttgccga tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct
    13441 ggcatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga
    13501 tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctatgcg
    13561 gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg
    13621 aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat
    13681 ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac
    13741 ggatgcgaat gacccgtgcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg
    13801 tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa
    13861 tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg
    13921 tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga
    13981 cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct
    14041 ttttcatgct attccttgaa aaggtcgcct gtcttttgat ctgctaaacg taacacataa
    14101 cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa
    14161 attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt
    14221 gcagcagctt acgcaaatag ttgagttcaa actgattacg tgcctcaaca aaggttggca
    14281 gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg
    14341 aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc
    14401 tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc
    14461 gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg
    14521 ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt
    14581 ccccgcgcgc catcgctttt ggcagatcac ggtgagtggc agaaataatc cgcacattga
    14641 tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca
    14701 gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt
    14761 ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat
    14821 gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa
    14881 atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg
    14941 tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt
    15001 ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg
    15061 tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga
    15121 caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag
    15181 aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa
    15241 atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt
    15301 cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt
    15361 cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata
    15421 aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca
    15481 ctctcgtgcg gcgtatccgg cgagaaattt ccagtcggtt tgcgggtcga gagctggcgt
    15541 tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg
    15601 tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa
    15661 cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac
    15721 agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg
    15781 cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt
    15841 tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga
    15901 tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc
    15961 agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt
    16021 ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat
    16081 gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt
    16141 cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga
    16201 catggacagc gcggagaaac cggagcgtac atatcgtacg tgaagatttc gagcactgcc
    16261 cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt
    16321 ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat
    16381 acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc
    16441 ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc
    16501 gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat
    16561 gtgaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt
    16621 aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga
    16681 gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg
    16741 tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac
    16801 ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcggctgctg ctatcaagaa
    16861 tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctggtca gccagtaatt
    16921 cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa
    16981 attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc
    17041 caaccgagcg taactcgctc ggtccactga acgaaacgct attgcccaga gaacgccctt
    17101 cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga
    17161 aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt
    17221 gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg
    17281 tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag
    17341 catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg
    17401 cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac
    17461 gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac
    17521 ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa
    17581 taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg
    17641 cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt
    17701 gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga
    17761 atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt
    17821 tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc
    17881 tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac
    17941 gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact
    18001 gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat
    18061 ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag
    18121 ataaacttat tagattgcaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt
    18181 aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga
    18241 tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc
    18301 caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc
    18361 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    18421 gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc
    18481 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    18541 gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga
    18601 gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt
    18661 tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg
    18721 ccacaactcg ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca
    18781 tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat cgcgtacacg
    18841 gtcattgaac aggattgact tcgcccaacc ttcaccggca cccagcacat caccgtagga
    18901 gaaaccaccg cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc cggtcagcag
    18961 gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg tggaaagctg ccgccatttc
    19021 aacatgcgag ttcacgccct gctcacgcag cacagcaact ttcggacgtg cgccagtggc
    19081 aatatacggt gctgccacat cttcgttgat atcgaacgac agtttcacat tcaggcccgg
    19141 atcggcgtcg tttgatttcg cctggtgctc ctggtcagca cactccgggt tgtcacgcag
    19201 gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtggtgc ggctttcgct
    19261 gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgccctac
    19321 ataatggaca caatcagcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc
    19381 agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc
    19441 gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga ccagcaaagg ccatttccgc
    19501 cagcgttacc agcaggccgc catcagagcg gtcgtggtat gcgagcagct tacgctgtgc
    19561 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacgtcgc gtacatctgc
    19621 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    19681 gcctttgccc aaatcaatca gcagcagtgt gttatcttcg gtagaaagct gtggcgtgat
    19741 ggtgtgacgc acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt
    19801 catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc
    19861 caccgggatc gtcaggccca gcgccggaca aagctcttcg cccacggctt taacggcttc
    19921 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    19981 aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc
    20041 cagacgggca gaggcggcga aatccagcag tgcaaccggc gcacgctcgc caatcgccat
    20101 cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    20161 ctgccacggc cccaccatct gatcgcgcgc taccatgccg gttacgctgc ggtcgccgat
    20221 ggtcaccagg aaggttttct ctgccacggt cggcagatgc agtacgcgtt tcaccgcgtc
    20281 tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc
    20341 gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt
    20401 atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    20461 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac
    20521 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg
    20581 ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgcccgccgt cgctcaccag
    20641 ttccggcatc gcgttagaaa gaccgccagc gccgacgtcg tggataaaca ggattgggtt
    20701 ggcatcacca agctgccagc aacggtcgat cacttcctgg cagcgacgct ccatctccgg
    20761 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgacctg acgccatgga
    20821 agacgccgct ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    20881 cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc
    20941 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc tgttcacttt
    21001 ttcttcataa gtacggaagt agccattcag tgccggacga ccaaattcgt tgttaaacgc
    21061 cgcgccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc gctcaggctt
    21121 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg aaacggagaa
    21181 accaaccaga ccagccttcg gcttcgcacc gcgtccggtg gcgccttcat cacgaatttc
    21241 gccgccggaa cccgtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    21301 ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc ccgtttcgtg
    21361 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    21421 agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcttga acagcgattt
    21481 cggctgctgt tcaccgtcga taatccagtc ggcattaaaa attttgtggc ggcagtgctc
    21541 agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt
    21601 gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt
    21661 agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgctggtaa ccggagtcgg
    21721 ttgatggtgg gcaaacaact gctctgcatc atctaaagca aaaaagaccg tttccatcat
    21781 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    21841 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc
    21901 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    21961 gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca ggcgttcaag
    22021 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    22081 gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    22141 gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    22201 gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac
    22261 gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg
    22321 ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac
    22381 ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat
    22441 tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg
    22501 ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag
    22561 cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg
    22621 ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggtgtaa
    22681 aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctc gataatggta
    22741 acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc
    22801 ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgagt cagtatcgcc
    22861 cgcgcacact gggcaacctg acggcggagc aactcaccgt tgcaccgggt cacgtggtgg
    22921 ttaacgatct ccaaaccctg aaagacacaa aattcccgga attaagctgg aaggtagacg
    22981 acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca
    23041 ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc
    23101 tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc
    23161 tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca
    23221 ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt
    23281 tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag
    23341 aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg
    23401 ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc
    23461 tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc
    23521 aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc tggtttgccc
    23581 tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa
    23641 ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga
    23701 aaccctatta cagcaagctg acttacggct acgctcgtgg acatgaagcc tacgcttatg
    23761 tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc
    23821 aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt
    23881 tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga
    23941 cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg
    24001 tcgagggttg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac
    24061 tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca
    24121 tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg
    24181 caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg
    24241 gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac
    24301 cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt
    24361 tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc
    24421 gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat tcgtggctaa
    24481 attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac
    24541 gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat
    24601 gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc
    24661 gcagcggagt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca
    24721 tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg
    24781 gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca
    24841 accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa
    24901 aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg
    24961 cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac
    25021 gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat
    25081 tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca
    25141 atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct
    25201 ctggactgaa atatcctgat taattttcgc tactacatag cctaacagat agatcatcac
    25261 ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag
    25321 cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt
    25381 tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg
    25441 cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt
    25501 taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg
    25561 cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg
    25621 caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt
    25681 tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa
    25741 acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga
    25801 taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga
    25861 tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc gcaacagtac
    25921 aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt
    25981 taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct
    26041 ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac
    26101 aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc
    26161 tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg
    26221 aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tacaatgccc gcccggtttg
    26281 tgttgttttg agagtttcct tatggcgttg ttaatcacta aaaaatgcat caattgtgat
    26341 atgtgtgaaa tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga
    26401 atgtgttgtt ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca
    26461 ccactttctt taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg
    26521 tgacatacca cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc
    26581 ggttttatat aaccctggct ggaaacctct tatacaaaaa agtcgattcg ccaacatcat
    26641 agatgatcgc caccttctgg cgagaaactc ctgatgcaat tagtcgcccg gcctgcgccc
    26701 attattctgg tgaaagtttg ggacggcgac cgccaattcg tccctatgcg cgagcagctt
    26761 ccagtcctgc ttttgttcgt tcaacaatca gttcacgttc catttcagcc agggcaccca
    26821 tcacatggaa gaaaaagcgt cccattggtg tgctggtatc aattgaatca gtcagactac
    26881 gaaagttgat gcctcgttcg cgcaactcct ccaccagcac gacaagatgc cgcatactgc
    26941 gccccagccg atccagcttc cagacaacca gtgtgtcgcc tgccgataat gtcctgagca
    27001 gttttttcag ccccggcctt tcggactttg tgccacttat cttgtcttca aaaatcagct
    27061 cgcatcctgc acagttcaac gcattacgtt gtagatcggt attctggtca tttgttgata
    27121 cgcgta
//
LOCUS       DAZOZD010000033        27107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOZD010000033 DAZOZD010000000
VERSION     DAZOZD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.033"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..924
                     /locus_tag="ACHUPB_001120"
     CDS             <1..924
                     /locus_tag="ACHUPB_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0757998.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHUPB_001121"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHUPB_001121"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0757999.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHUPB_001122"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHUPB_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0758000.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="ACHUPB_001123"
     CDS             3885..4280
                     /locus_tag="ACHUPB_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0758001.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="ACHUPB_001124"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="ACHUPB_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0758002.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="dolP"
                     /locus_tag="ACHUPB_001125"
     CDS             4900..5475
                     /gene="dolP"
                     /locus_tag="ACHUPB_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0758003.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="ACHUPB_001126"
     CDS             complement(5589..6629)
                     /locus_tag="ACHUPB_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0758004.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="ACHUPB_001127"
     CDS             complement(6702..7337)
                     /locus_tag="ACHUPB_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0758005.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHUPB_001128"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHUPB_001128"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0758006.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="ACHUPB_001129"
     CDS             complement(7963..8406)
                     /locus_tag="ACHUPB_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0758007.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHUPB_001130"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHUPB_001130"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0758008.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="ACHUPB_001131"
     CDS             complement(8746..9249)
                     /locus_tag="ACHUPB_001131"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0758009.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="ACHUPB_001132"
     CDS             complement(9243..9767)
                     /locus_tag="ACHUPB_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0758010.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="ACHUPB_001133"
     CDS             9976..10971
                     /locus_tag="ACHUPB_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0758011.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="ACHUPB_001134"
     CDS             10980..11858
                     /locus_tag="ACHUPB_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0758012.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="ACHUPB_001135"
     CDS             12064..13071
                     /locus_tag="ACHUPB_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR0758013.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHUPB_001136"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHUPB_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0758014.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHUPB_001137"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHUPB_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0758015.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHUPB_001138"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHUPB_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0758016.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHUPB_001139"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHUPB_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0758017.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHUPB_001140"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHUPB_001140"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0758018.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHUPB_001141"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHUPB_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0758019.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHUPB_001142"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHUPB_001142"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0758020.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHUPB_001143"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHUPB_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0758021.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHUPB_001144"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHUPB_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0758022.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHUPB_001145"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHUPB_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0758023.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHUPB_001146"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHUPB_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0758024.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="ACHUPB_001147"
     tRNA            complement(26829..26905)
                     /locus_tag="ACHUPB_001147"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6421 a         7518 c         6811 g         6357 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aagtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc cccctta
//
LOCUS       DAZOZD010000034        26866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOZD010000034 DAZOZD010000000
VERSION     DAZOZD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.034"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(85..807)
                     /gene="baeR"
                     /locus_tag="ACHUPB_001148"
     CDS             complement(85..807)
                     /gene="baeR"
                     /locus_tag="ACHUPB_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0758025.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(804..2207)
                     /gene="baeS"
                     /locus_tag="ACHUPB_001149"
     CDS             complement(804..2207)
                     /gene="baeS"
                     /locus_tag="ACHUPB_001149"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0758026.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2204..3619)
                     /locus_tag="ACHUPB_001150"
     CDS             complement(2204..3619)
                     /locus_tag="ACHUPB_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0758027.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3620..6697)
                     /gene="mdtC"
                     /locus_tag="ACHUPB_001151"
     CDS             complement(3620..6697)
                     /gene="mdtC"
                     /locus_tag="ACHUPB_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0758028.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6698..9820)
                     /gene="mdtB"
                     /locus_tag="ACHUPB_001152"
     CDS             complement(6698..9820)
                     /gene="mdtB"
                     /locus_tag="ACHUPB_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR0758029.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9820..11061)
                     /gene="mdtA"
                     /locus_tag="ACHUPB_001153"
     CDS             complement(9820..11061)
                     /gene="mdtA"
                     /locus_tag="ACHUPB_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR0758030.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11340..11396
                     /locus_tag="ACHUPB_001154"
     CDS             11340..11396
                     /locus_tag="ACHUPB_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0758031.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11667..11723
                     /locus_tag="ACHUPB_001155"
     CDS             11667..11723
                     /locus_tag="ACHUPB_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0758032.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11996..12055
                     /gene="ibsA"
                     /locus_tag="ACHUPB_001156"
     CDS             11996..12055
                     /gene="ibsA"
                     /locus_tag="ACHUPB_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR0758033.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12276..12935
                     /locus_tag="ACHUPB_001157"
     CDS             12276..12935
                     /locus_tag="ACHUPB_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0758034.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12932..13693
                     /gene="pphC"
                     /locus_tag="ACHUPB_001158"
     CDS             12932..13693
                     /gene="pphC"
                     /locus_tag="ACHUPB_001158"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR0758035.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            13690..15630
                     /gene="yegI"
                     /locus_tag="ACHUPB_001159"
     CDS             13690..15630
                     /gene="yegI"
                     /locus_tag="ACHUPB_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR0758036.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(15643..16995)
                     /gene="yegD"
                     /locus_tag="ACHUPB_001160"
     CDS             complement(15643..16995)
                     /gene="yegD"
                     /locus_tag="ACHUPB_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0758037.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            17129..17977
                     /gene="alkA"
                     /locus_tag="ACHUPB_001161"
     CDS             17129..17977
                     /gene="alkA"
                     /locus_tag="ACHUPB_001161"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR0758038.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(18087..21404)
                     /locus_tag="ACHUPB_001162"
     CDS             complement(18087..21404)
                     /locus_tag="ACHUPB_001162"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0758039.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            21722..22363
                     /gene="udk"
                     /locus_tag="ACHUPB_001163"
     CDS             21722..22363
                     /gene="udk"
                     /locus_tag="ACHUPB_001163"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0758040.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            22455..23036
                     /gene="dcd"
                     /locus_tag="ACHUPB_001164"
     CDS             22455..23036
                     /gene="dcd"
                     /locus_tag="ACHUPB_001164"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0758041.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            23058..24911
                     /gene="asmA"
                     /locus_tag="ACHUPB_001165"
     CDS             23058..24911
                     /gene="asmA"
                     /locus_tag="ACHUPB_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0758042.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(25185..26768)
                     /locus_tag="ACHUPB_001166"
     CDS             complement(25185..26768)
                     /locus_tag="ACHUPB_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0758043.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         6371 a         7188 c         7225 g         6082 t
ORIGIN      
        1 gtcagtttgc tgaccatgcg tgggataatg ttgccggatc tggtctgaac gccatctccg
       61 gctaacaaag tcatgtcgct aaaactaaac gatgcggcag gcgtcggctt cccagcggta
      121 accaacgccg taaacggcgc ggataaatga ctgttcggcg tcgagagatt ccagcttgcg
      181 gcgcaggttt ttaatgtggc tgtcaatggt gcggtcggtt actacgcggt agtcgtcata
      241 aagatgattg agcaattgct cgcgggagaa cacttttcct ggttcatgag agagcgtttt
      301 caacagacga aattccgcag gcgtcaggtc aagcatttta ccgcgccatg aagcctgaaa
      361 acgaccttcg tcgataatca acgggctttc ggcatcctgt tgctgcaatt cgcgctgcgg
      421 tttgcaacgg cgcaaaatgg ttttgacgcg cgctaccact tcccgtgggc tgtacggctt
      481 gcagatgtag tcatctgcgc caatctccag tccgagcagg cggtcgatct cttcgatttt
      541 tgccgtcacc atcacgatag gaatgtcaga aaaacgacga atttcccggc acagcgtcaa
      601 gccatcggtg ccagggagca tcagatccaa caggatcaga tccggtggtg tctggcgcac
      661 atacggcagt acctgatcgc cgtggctgat aagcgtcggc gcatagctcg cagcacgcag
      721 ataatcaatg agcaactgcc ccagcttcgg ttcatcttcc acgatcaaaa tacgcggtgt
      781 gttttcgtcg attggtaact cggtcatact tctctctgta aatcccgttc cagcggtaac
      841 tctactgtaa tgcttacccc gccaaaaggc gaatgggcag caataatgcg gccattatgt
      901 gcttcaacaa tgttcaggca aatcgccagc cccagcccgg aaccaccgct ggcgcggttg
      961 cgggaacctt cggtgcgata aaaacgttca aataattttt gtagctgctc gtcactgaca
     1021 cctggcgcac tgtcggcaaa ggtcaggcgc accgttttgt cgtgctgctc ggcagagatt
     1081 ttcaggctgc cgccgctgtc agtgtagcgc aggctgtttt caagtaaatt attgaataac
     1141 tgcattaaac ggtcgcgatc gccaaatacg gtaatactgt ctggcaggga aaattgcagt
     1201 ttcaggccac ggctggcgaa tcgttcgcga aatgcgccgc ccgccacttc cagcagtggg
     1261 atcaaatcta ccggtgcttt ttgataggcg agggcgcctt catcagacat cgacaactga
     1321 tggagatcgt caaccagttt ggtcagtgta ccgacctccg cctgtaaaga cgccaccgtc
     1381 tccggcgtga atttgcgcac gccatcctga atagcttcca gctcaccgcg cagcaccgct
     1441 aatggcgtac gcagttcgtg agaaatatcg gccataaaat cgcgccgcat ttgctggttt
     1501 ttctccagtg tgctggcaag ctggttgaag tcttgcgcca gtttgcccag ttcatcttca
     1561 ctggtgggcg ttacgcgggt agtgaaatcg cccgccgcca gtttgtgcgt gccatcgaca
     1621 agtcgtttta ccggtgccag taaaccgcgc gccagcagaa aagtggcaag tgccgcgagt
     1681 aacgttgcca gggcgacaat caaccagctg gtttgccgct gttgtttatc gaaattgata
     1741 tcagtattgc gcgttaaccg ctcaacgggg gaggcgatca ccgcgccaac ttccgcaccg
     1801 ttgaccagaa tgggtcgccg tgtaccgtca ggtggaatcg gcgctcgcgg accaaccagc
     1861 actttgttgt tttgatcaac cacccagaac tgggtacgcc agccgtgcgg tggcataccc
     1921 gggccgggtt tatcttccga attatcgtgt tcaaatgaac gcaggatctg aaagacaaag
     1981 cgatcattgt tgcgcaggaa gcgccagttg ccatgctgcg catactgctc gccaagcgca
     2041 tcacttaaca gttgtaatcg ctgttcatta ccatgcttga tgtaatcaat aaagccacgc
     2101 tcaaaactga tacgcaccgc ccagtgcata ctgatcagca aaacaatgca ggtggcgaaa
     2161 atcgccagaa acagtttgcc ggtaatacca ggtcgccaga acttcattgc gcgctccttt
     2221 ttcgccgcga aatagctaca ttttgatgcg tatcgttcgg cactctggca aagatgaacg
     2281 ccggaagggc gatgataaac gccatgctaa gccaggtgta cataaagacg gtttgtgtgg
     2341 tgccgctgtc gacgctgaca tgctgcgaac caaaaagtcc cagcaacagc ccggcgatgg
     2401 tgacgccgat actcatcgac aattgcataa tcatcgacag cagactgttg ccgctgctcg
     2461 ccagattgtc cgggagatct ttcagcgtca gggtgttcat ggaggagaaa cgggtcgagt
     2521 tgaccatccc ttgtaaaaac aggacgaacg gcaaaacgta gtaccagccc aacagagcgg
     2581 tagtcataaa caacagggtg atcagcgaca gacccagcgt ggtcgctacc agtacccgac
     2641 gataaccaaa gcgattcacc acctgaacca caattcgctt cattcccatg ctgccaagta
     2701 ccatcgggat catcatcagt ccggcatgaa acggcgagaa accgaggcca atttgcagga
     2761 aaaccggtgt cataaagggc aacatgccac tgccaatacg tccggcaaag ctccccgcca
     2821 ggcccagcga aaaggtacga gtgcggaaca gtttcagact gaacagggcg cggttgttat
     2881 ttctggcgtg cagcagataa agcaccagtg ccaccacgcc aactgcgacc aggccagcaa
     2941 tcgccagcgg cgataaacct gtacctttac tgccgtccag cgccagggtt aatacagcca
     3001 tgccaaccgc cagcaataaa aagccggaaa gatcaaagcg ccgcgtctgc atggtgtagt
     3061 tcggcattaa cagcaatgtg gcgatcgcac cgataatccc caccggaatg ttgatcaaaa
     3121 agatccagtg ccacgacgcg tactccacca gcagaccgcc gagcgccgga ccgagcagcg
     3181 gaccgacctg accgggtaac gtgacaaagg tcatcgccgc catatattgc tcgcgcggta
     3241 cgattttcat caccgtcaac ctgccgaccg gcaccatcat cgcaccgcca acgccctgta
     3301 acgcgcgtgc cagcaacagt tcgttcagcg tgccggaaag cgcgcaaaac agtgaaccga
     3361 gagtaaacag cacgatggcg gtaaagaaaa tattgcgcac gccgactttg tccgccagcc
     3421 agccgctggc gggcagcatc accgccacgg tcagcacata agagacaatg accatgtgca
     3481 tatgcaacgg actttccccg aggctttgcg ccattgaggg aagggcggtg ttaacgatgg
     3541 tggtgtccag cgactgcata aagaagccga aagccacaat ccacaattgc cagcgggtgc
     3601 tgtcgggaag ttctgtcatt tactcggtta ccgtttgttt aggtttacgc gaaaaacgca
     3661 gccgcagacg gtcgaaaaag agatacacca ccggcgtggt atacagcgtc agaagctggc
     3721 tcattaccag tccaccgaca atggtgatcc ccaggaggtg ccgcagctcc gagccgtcac
     3781 cgcccgacaa caccagcggc agcgcgccaa acagcgccgc cagggtagtc atcataatcg
     3841 ggcgaaaacg cagcagacag gcctggaaaa tagcttcctg cggcgtcagg ttaccgtgcc
     3901 gttgggcttc gagcgcaaaa tcgaccatca taatggcgtt tttctttacg atgccgatta
     3961 atagcatgat ccctatcagg gctattaggc tgaacggggc attgaacagc tccagcgcca
     4021 gcagcgcacc aacgcccgcc gagggcaggg tggagagaat cgtcagtggg tgtacgtaac
     4081 tctcgtaaag gatacccagc acgatataca ccgtggcgat ggcggcgata atcaggatca
     4141 cctgtgagtt catcgtctcc tggaacacct gcgctgttcc ggcaaaactg ccgcgcaccg
     4201 tcgaaggcac accaagctgg gtcattgcgc gatcgatcgc cgcactggcg tccgagagcg
     4261 attttccggt cggcaggttg aacgagatgg tcgaggccgc cgataatccc tgatgattca
     4321 ccgacagcgg agcgttcgcc ggttgccatt tagcgaaata cgacagcggg atcgctttgc
     4381 cttcgttatt gataacgaac attttttcca gcgcactgat gtcctgggta tagcgcggat
     4441 ccacttccat caccacttta tactggttca tcggctggta aatggtcgag atttgccgct
     4501 gaccgaaggc gttatttaac aggctgttgg cggcctgcac gtcgattccc agccgtgcca
     4561 tggtgtcgcg gtcgtaaacc agattcatct ccgcgccgtt atcctgctga tcggagttca
     4621 cgtccgccag ttccggcaac gtcgccagtt ttttgcggat tttcggctcc cattcgcgca
     4681 gtgccgccag gtcgtcggat aacaacgtgt actggtagct ggcgttcgac tgacgcccac
     4741 caacgcgaat atcctgtacc gccatcagga acagattcgc ccccggttct ttcgccagtt
     4801 ttacgcgcag acggtcgata atttgctgcg ccgtttcgct gcgttcgtcg cgtggcttga
     4861 gggtgataaa catcatcccg ctgttcactc gcgaaccgcc tgtaaagccg gtgacattat
     4921 ccactgccgg atcgtcacgg ataattttca tgaaatcctg caacttaccg cgcatcgcct
     4981 gaaacgaaat actctgatcc gcctgaatcc cgcccatcaa cacgccagtg tcctgctccg
     5041 ggaagaaggt tttcgggatc gagatataca gccagatatt cagcgcaatg gtgccaagca
     5101 gcaccacgcc caccagacgg gtatgattga gcacccattt cagtgacttg ccgtagcctt
     5161 gttgcagggc taccaacatg cgaccaaaac cacgcagtcg cttttgctcg cgcggcttgc
     5221 tggctttcag catccagcca cacatcattg gcgttaatgt cagagaaacc agcaacgaaa
     5281 taccaatggc gacagaaagc gtcacggcaa actcgcgtaa cagtcggccc ggcaatccgc
     5341 ccatcaacag cagcggcagg aacaccgcca ccagtgacag actcatcgac agcaccgtaa
     5401 aaccgacttc gcgagtacct tgcagtgcgg cttgcaacgg tttcattccc gcttccagat
     5461 gacgtgcaat gttttccagc accacgatgg cgtcatccac cacgaaacca gtagcgatgg
     5521 tgagcgccat taacgaaagg ttattgagac tgaatccgca caggtacatc gccgcaaacg
     5581 taccaatcag cgaaaccggc accgaaacgg cgggaataat agtggcgcga cccgagcgca
     5641 ggaataaaaa caccaccaga atcaccagcg ccaccgagat aatcagcgtt tgctcgactt
     5701 cttccagcga ggcgcgaatg gtgggggagc gatcctgggc aatttgcaga tcaatcgccg
     5761 ccggaatggt ttcctgcaac tccggtaatt ttgcccggat gctgtcaacc gtctggataa
     5821 tattggcttc cggcagtttg cggatcatca gtaaaatagc cggtttggcg ttggtcatcc
     5881 cggcgttgcg cacatcctgc actgagtcgg tcaccgtcgc cacatcgccc agacgaaccg
     5941 cgccgccgtt gttgtagtga ataatcaacg gctgatattc agcggcggtt tttagctcat
     6001 cattggtctg gatctgccag cggtgagtgc catcttccag cgcgccctgc ggtttacgca
     6061 cgttggcatt gctgatggcg gtgcgtacgt cgtccagcga cacgccctga ttaaacagcg
     6121 cctgcggatt cagcccgacg cgtacggcgg gcagtgagct gcctccgaca tcgacatcac
     6181 caacaccgtc gatttgcgaa atcgtcggag ccagctgcgt cgaggcgaaa tcgtacagtt
     6241 caccctgcga ataagtatcg gatgtcagcg tgaggatcat aattggcgca tccgacgggt
     6301 tcgctttgcg ataggtcgga cggctgggca tcccactggg cagcaaactt tgtgcagcgt
     6361 tgatcgccgc ctgcacatca cgcgctgcgc cgttgatgtc ccggtcaaaa tcaaactgta
     6421 aaataatacg tgtgctgccg agcgaactgc tggaggtcat ttcgctgacc ccggcaatgc
     6481 gcccaagcga gcgctccagc ggcgtggcaa cggaagacgc cattgtttct ggtgacgcac
     6541 cgggcagcga ggcgctgacc ataatcaccg gaaaatcgac ctgcggcagc ggggcgaccg
     6601 gcagcatacg gaagcccagt atgccgcaca gggtaatggc gaccgacagt aaaatcgtcg
     6661 ccaccgggcg gtaaatgaag agggcaaaaa acttcactta cgcctcctct tcatggcgag
     6721 caaagcgact tttggtccac aatgccaggc ggtcgaacag cagataaatc accggcgtgg
     6781 tgaacagcgt cagcacctgg ctgacaatca gaccgccgac catgccgata cctaacggac
     6841 gacgcagttc cgcgccgacc ccggtactca acatcagcgg cagcgcgcca agcagagccg
     6901 ccagggtggt catcaggatc ggacgaaaac gcaacagaca agcctggtag attgcatcgc
     6961 gcggcgacat gccttgctcg cgctcagcag ccagcgcgaa gtcgatcatc atgatggcgt
     7021 tcttcttcac aataccgatc agcaaaataa tgccgataat cgcaatcaca tccagttcgc
     7081 taccggcaat cattaatgcc agcagcgcgc cgacccctgc ggtgggtagc gtcgagagaa
     7141 tggtgatcgg gtgaataaag ctctcgtaca gaatgccgag cacgatatac atcgccacca
     7201 ccgccgcgac aatcagccag acagtgctgc ccagcgccga ctggaaggcg agggtgctgc
     7261 cctggaattg cgtggtgata tccaccggca gattcagcgt cttttcggtg tccataatct
     7321 cctgcaccgc atcgcccagc gaatagttat ccggcacgtt aaaggagatg gtcgttaccg
     7381 ggaactgatc cagatggttg atggaaagcg gcgcaaaacg ctgttcaact ttggcaattg
     7441 agcttaacgg caccacgccg ccgtcgctac tggtcaggcg aatggtatcc agcgccgcga
     7501 ggcccggggt aatttcggtg ttgtgctcca gcactacgcg atactggtta gcctgagtat
     7561 aaatagtgga aatcagtcgc tgaccaaacg cgttgtacag ggcgttatcg acatccgcca
     7621 tgctgatccc cagacggctg gcgctgtcgc gatcaacatt gacatacgcc accagccctt
     7681 ggtcctgcca gtcgctggag acatcagaaa gctgtggcag ttgctggagt ttttccatca
     7741 actgtggcac ccaggtactg agcgcatcca gtgaggtggc ctgcaaggta aactggtact
     7801 gggtgcggct gacctgagta tcaatagtca gatcctgcgt tggttgcagg aagagatcga
     7861 cgcccggcac tttatctacc gccgtttgca gacgggcgat gactttttgc acccgatcat
     7921 cacgttcatc caacggtttg aggttgattt gtaaacgtgc actattcagc gacgggttag
     7981 tgccatcaac gccaacaaat gaggtcaggc tttgcactgc cggatcctgc aaaatcacgt
     8041 ccgcgacctg gcgttgtcgc tgggccatat tggtaaagga gctggattgc ggtgcctgca
     8101 aagtgccctg aataatgcca ttgtcctgta ccgggaagaa acctttcgga atgaacaccc
     8161 acagcagcac gctaagcagc agcgtgctga gtgccacgct taaggtcagc cacggatgat
     8221 tcagcacttt cgccagtcca cgaccatagg cggcgattat cctgtcgaac attttttccg
     8281 aggcacggga gaagcggttc tgtttacgca acgactcctg gctgagcatc cgcgcgcaca
     8341 tcatcggtgt cagggtcagc gacaccaccg ctgagatcaa aatcgctacc gccagggtaa
     8401 tagcaaattc gcggaacagt cgcccgacga tatcgcccat aaacagcagt gggatcaaca
     8461 ccgcaatcag tgagaaggtc agcgagataa tggtaaagcc gatttcacct gcgcccttga
     8521 gcgccgccgc caacggtttt tcgccttttt cgatatagcg ggaaatgttt tcgatcacca
     8581 cgatggcgtc atcgaccacg aatccggtgg cgatagttaa cgccatcagt gtcaggttat
     8641 tgattgaaaa atcgagaaac accataaccg cgaaagtgcc gattaacgac agcggtacag
     8701 caacaccggg aatgatggtc gccggaatat tgcgcaaaaa caggtagata atcatgacta
     8761 ccagcgcgat agccatcatc aattcaaact gagtatcatc gacggatgcg cggatattgg
     8821 tggtgcgatc ggaaagcact gtcaccttca ccgatttcgg cagactctca gtgagctgtg
     8881 gcagcatctg ccgaatgctg tcggcggtgg agataatgtt agcaccgggc tggcgctgaa
     8941 cattcatcac aatggcctgt tctttgttcg cccacgcgcc gagccagctg ttttctgcac
     9001 cttgctctac agttgcgaca tcgcccagac gaattggcgc gccgttctgg taggcgatga
     9061 ttagctggcg atactcttcg gcggattgca tctggtcgtt cgcggaaagc gtgaccgcac
     9121 gggaagggcc gtcgaggcta ccttttgccg agttaacgtt agcgccggta atggcggtgc
     9181 gcacggtttc gctggtcagg ccgagggcgg caatcgcctg agcgttaagt ttgacgcgaa
     9241 cagccggacg ctgaccgccg gaaagcgtca ccaggccgac gccggaaatc tgcgagattt
     9301 tctgcgcgac gcgggtttcc accatatctt ccacttgcgt catcggcatg gcggttgagg
     9361 tgacggcgag cgtcatgatc ggcggatctg ccgggttcac tttgctgtaa accggcgggt
     9421 taggcagatc gctcggcaac aagttggtcg cagcgttaat cgcggcctgc acttcctgct
     9481 cggcgacatc gagcggtaat gttagctgga actgcaaagt gataactgac gcaccgccgg
     9541 aactttgcga cgacatctgt ttcaggccag acatctgccc gaactggcgt tcgagcggcg
     9601 cggtaacggc agaggtcatg acatccgggc tggcacctgg gtagagcgtg accacctgaa
     9661 tggtcggata gtccacttcc ggcagcgccg aaacgggcag ggcgcgataa ccgataatcc
     9721 cggcgagtaa gatcgccacc atcagcagcg tggtggcgac aggacgcata ataaacaggc
     9781 gcgacgggcc gcctgtgctg ctcgggggta acacctgcat caggagcgtg ctcctttttt
     9841 cgcgtattcg cggctggtgg ctttctcttc cggagtggtg gcgctctggg cttccaccac
     9901 ttccactttc gccccttcgg tcaggcgatc aatgccgtct gtcaccacgc gatcgcccgc
     9961 agaaatacct gcacggatca ccactttctg actgtcctga atgcccggcg tcaccagatg
    10021 tttgctgacc ttgttttcgc tattcagcac ccagacaaaa tggccttcat tgcccatttg
    10081 cagggcggct gttgggatca ctacggcgtt ttgttcggtg tcgactaaca tgcgcgcgtt
    10141 aacaaactga ttgggaaaca gcgcatcatc ctgattatta aagcgtgctt tcactttaat
    10201 cgtaccggtt gtggcatcga tttggttatc gaggcttaac agcgtgcctt cacttaattt
    10261 cttcgaattg gtgcgatccc aggcttctac caccagcgtt tttccggctt tttgcgcctg
    10321 cactacagta gcgatatcgc tttccggcag ggtaaagact aaatcgatag gatgcgtctg
    10381 ggtgatcacc acaattccgg tggtatcacc actggagatt tggttaccaa catcaacctg
    10441 cttgagacca acgcgaccat cgactggtgc ggtgatgcgg ctccagtcga gttgcaactg
    10501 cgcgctggcg acgcttgctt catcagcctt aatggtgcct tcggtttcac tgaccagcgc
    10561 ctgttgggca tccagctcct gacgggagac gagattggtt tttgccagtt gctgataacg
    10621 cgccagatca cggcgggcgt tagcaagcgt ggctttatct tttgccagtt ggccctgggt
    10681 ttgtgctaat gcaactttga actggctggg gtcaatttct gccagtaaat cgcctgcttt
    10741 gacctgctgg ccttcctgga aatgtaacgc catcagttga ccgtccacgc ggctgcgcac
    10801 cgtaacagta ttagcggcga taatggtgcc aagcccggtg aggtaacgcg gaactgcctg
    10861 ttctacggcg gtcgccgcct gaaccggggc taatgggccg gaacgcatac cacggcgacc
    10921 acccgctggc gattgctgcg cttgtttcgt cgcccctggg gctgcactct gggagtcatt
    10981 gcggccttgc cagaaccaga atgcggcgat ggcggcgata accaccacga ttacccaacg
    11041 ggatttataa ctgcctttca tcgttaagag tttctcttcc tgaaacgttt cgcggaatga
    11101 tactagtgta gccagccaat aagggagaaa aatggaggaa ttatgaaaca ctgtcgggaa
    11161 tcgtctgaat gacgggcaca tttgcgagca cgcatccagt aataacacag gaaactattt
    11221 tatctacgcg ttagcgatag actgcttgca tggcgaaagg aggtaagccg ccgatttcag
    11281 cgggacgctg aaacgggaaa gcccctcccg aggaaggggc cataaataag gaaagggtca
    11341 tgatgaagct actcatcatc gtggtgctct tagtcattag ctggccagct tactaaggct
    11401 accagggcgg gggaaacccc gctctaccct cactcctgaa agtatgcctt cacgataaga
    11461 ttgtcaatct gcaggctttg cagtctgcga tcctgccagc aaatattctt tgcgagacgt
    11521 tacgcaataa tcacactgga aactatttta tccgcaagtc agcgatagac tgcatacagg
    11581 gcgaaaggag gtaagccgac gatttcagcg ggacgctgaa acgggaaagc ccctcccgag
    11641 gaaggggcct taataaggaa agggtcatga tgaagctact catcatcgtg gtgctcttag
    11701 tcataagctt ccccgcttac taagactacc agggcggggg aaaccccgct ctaccctcac
    11761 tcctgaaatt atgccttcac gataagattg tcaatccgca ggctttgtag tctgcgatcc
    11821 tgccagcaaa tattctttgc gagtcgttac gcaataatca cagatgaaac tattttgttc
    11881 acgcgttagc gatagactgc attcagggcg aaaggaggta agccgacgat ttcagcggga
    11941 cgctgaaacg ggaaaccctc tcccggagaa gagggctttt aataaggaaa gggttatgat
    12001 gaagcacgtc atcatactgg tgatactctt agtgattagc ttccaggctt actaagaaca
    12061 ccagggggag ggggaaacct ctccctaacc ctcactcctg aaattatgtc actccgccct
    12121 tagtgtcaat cgacggctct ccgttcttgc gaggcgaata gcaaaatttt ctgcgccgac
    12181 gggcctaatt ctgaaagttt cttcgtttgc tcttatttca ttattgcaat cggtatgata
    12241 ccggacctcg ataattctct aagaaaggat ttcttatgag cgaacaaatc acgtttgcca
    12301 ccagcgattt tgccagtaac ccggaaccac gttgcccctg cattttgctg ctggatgttt
    12361 ctggctcgat gagcggcaga cctattaatg aacttaacgc cggattggtt acctttcgtg
    12421 atgaactgct tgctgatccg ctggctttaa aaagagtgga actcggcatt gtgacgttcg
    12481 gcccggtgca tgtggaacaa ccctttacca gcgccgctaa ctttttcccg cccatcctgt
    12541 ttgcccaggg ggatacgcct atgggggccg ccattaccaa agccctggat atggttgaag
    12601 agaggaaacg tgagtaccgc gcaaatggta tttcgtatta tcgcccgtgg attttcctga
    12661 tcaccgatgg cgctccaacc gatgagtggc aggctgccgc caacaaagtg tttcaggggg
    12721 aagaagataa gaagtttgcc ttcttttcca tcggtgttca gggggcggat atgaagactc
    12781 tggcacaaat cagcgttcgt cagcctttgc cactgcaggg attacaattc cgtgaactgt
    12841 ttagctggtt atccagttcg ctgcgttcgg tttcccgctc cacgccggga acggaagttg
    12901 tgctggaagc gccaaaaggc tggacgtcag tgtgagctgg cgtctggttt atgcatccgc
    12961 tgttggaaca tcgcacatta gtgctgattt gccttgtcag gatgcctgcc agatgcaggt
    13021 tgtctggtta aacgaccagc aaccattact ggtgatgttc cttgctgatg gcgcaggtag
    13081 cgtttcgcag ggaggagaag gcgcgacgct cgccgtcaat gaagcgatgg cgtttatggt
    13141 gcaaaaagta caggacggtg aattggggct taatgatgtc ctcgccacaa atatggtact
    13201 gactattcgt caacgacttt ttgccgaggc agaagccaaa gcattagcag ttcgcgattt
    13261 tgcctgcacg tttctgggat tgatttcatc gcctgacggc accttgatta tgcagattgg
    13321 cgatggcggc gtggtggtag atttgggtca cggtctgcaa ttgcctctca cgccgatggt
    13381 gggtgaatat gccaacatga cgcacttcat taccgacgaa gatgccgttt ccagactgga
    13441 aactttcacc agcactgggc gtgcgcataa agttgcagca tttacggatg gtattcagcg
    13501 gctggcgtta aatatgctgg ataattctcc tcatgtgcct ttctttaccc cgtttttcaa
    13561 tggactggcg tcagcaacgc aggaacaact cgatttatta cctgaattgt taaagcagtt
    13621 tttgtccagc ccggcggtaa acgagcgtac ggatgatgat aaaacgttgg cactggcgct
    13681 gtggttacca tgaaacccac tttatatact gctactggtg agtgcgttac gccaggccgt
    13741 gaactgggca aaggcggtga aggcgcggtt tatgatatca atgagtttgt cgatagcgtc
    13801 gccaagattt atcacacgcc gccacccgcc ttaaaacagg acaaacttgc ctttatggct
    13861 gcgacagctg acgcgcaatt gttgaattat gtcgcctggc cgcaggcaac gcttcacggt
    13921 ggacgaggcg gaaaagttat cggttttatg atgccaaaag tttctggtaa agaaccgatt
    13981 catatgatct atagcccggc acatcgtcgt caacgttacc ctcattgtgc gtgggatttt
    14041 ctactctatg ttgcgcgcaa tattgcttca tcttttgcta cggttcacga gcacgggcac
    14101 gtcgtgggtg acgtaaacca gaacagtttt atggtaggtc gcgacagcaa agtggtgttg
    14161 attgatagcg actcctttca gattaacgcc aatggcacac tgcatttatg cgaagtcggc
    14221 gtgtcgcatt ttacgccgcc agagctacaa accttgtcgt catttgtcgg ctttgaacgc
    14281 acagaaaatc acgataattt tggccttgcg ttgctgattt ttcacgtctt gtttggtggc
    14341 cggcatcctt attctggtgt gccgcttatc tctgatgcgg gtaatgcgct ggagacggat
    14401 attacccatt tccgttatgc ctacgcgtca gataatcagc gacgtggttt aaaaccgccg
    14461 ccacgatcga ttccgctatc gatgttaccg agtgatgttg aagccatgtt tcagcaggcg
    14521 tttacggaaa gtggcgtggc aaccgggcgt ccgacggcta aagcgtgggt atcagcactg
    14581 gattctctac gccaacagtt aaagaaatgt accgtttcgg caatgcatgt ttatcccggt
    14641 catttggctg actgcccatg gtgtgcgctg gataatcaag gtgttatcta ttttattgat
    14701 ctcggcgaag aggtcattac caccggcggg gattttgtgc tggcaaaagt ctgggcgatg
    14761 gtgatggcgt cagtagcacc gccagcattg caactgccat tacccgatca tttccaaccg
    14821 actggcaggc cgcttccttt aggcctgtta cggcgcgaat acatcattct gcttgagatc
    14881 gcactgtcag cattatcgct gctgctttgc ggccttcagg cagaaccgcg ttatattatt
    14941 ttggttcctg tgctggcggc tatctggatt attggcagtt tgacaagcaa agcgtacaaa
    15001 gcagaagttc agcaacgccg tgaggcattt aatcgtgcga aaatggacta tgaccattta
    15061 gtccgccaga tccagcaggt aggcggcctg gaaggtttta tcgccaaacg gacgatgctc
    15121 gaaaaaatga aggacgaaat tctcgggtta ccggaagaag agaaacgcgc tctggcagca
    15181 cttcatgaca ctgcaaggga acggcagaag cagaagtttc tggagggatt ttttattgat
    15241 gttgcctcta ttcccggcgt tggccctgcg cgtaaagcgg cgttacggtc ttttggtatt
    15301 gaaacagccg cagatgttac ccgtcgtggc gttaagcaag tgaaagggtt tggcgaccat
    15361 ctgacccagg cggttatcga ctggaaagcg aactgtgaac gccgttttgt gttcaggccg
    15421 aatgaagcga taacgccagc agacagacaa gcggtaatgg cgaaaatgac cgccaaacga
    15481 catcggctgg aatcggcgtt gacagtcggc gcgacagagt tgcagcgatt ccgccttcat
    15541 gctccagcac ggaccatgcc gttgatggaa ccgttacgtc aggcagcaga aaaactggct
    15601 caggcgcagg cagatttaag ccgctgctga gccatttttc aattaacgaa acaccacttc
    15661 cgcccagcgt gccagcccgg cggtgacgga gccaaagtca tcgccgcctg caatcggaat
    15721 gcccggcaac tgttctgcca gcgctttttt aatctgcgga gaacgggcgc taccgccggt
    15781 caggtagata acgtccggtt tttcctgggc gttatccagg gccagttgca cctgttccag
    15841 aatccgcgcc agtggctggc tgagggcgct ttccagccct cgctggctaa tcagcgtagc
    15901 cagttcattg ctgataaacg gcagtgaggc gcgggtttcc gctacgcttg aaagagcaat
    15961 tttgcactct tctgcgctac gcaccaggcg atagcttaaa cgctgacgcc agactttctg
    16021 taacagggcc actttttccg gttcgcgggc atcgcgtacc agatcgttaa gcagacgacc
    16081 gttggcacta ctgtagaaat cactctgcgc aggtacgtcg ttgatggcaa ccgcattcca
    16141 ccacggcagg atcggcaggg cgatgccttt ttcggtttcg ccacccatac ccagcagtgg
    16201 catcaggttt ttaaacgcca gggcgatatc cagatcgtta ccgccaatac gacaaccact
    16261 gtgacccagc agactggctt cacgatcgag acgcgaacgc cactgcggcc ccatcagcag
    16321 caatgaacag tcagtcgtac caccgccgat atccaccacc agcacccgtt tttcttcctg
    16381 caaggtggct tcgtaatcca gcccagccgc gatcggctcg tactggaata ccacgtccct
    16441 gaatccggca cgcttcgccg cgcgttccag aatctcttgc gcctgggcgt ttgcttcatc
    16501 gccgcccagc ccctggaagt tgatcgggcg accaatcacc gcctgagtaa tcgcttctgg
    16561 cagctgcgcc tgcgcctgct ggcgaatgtg caacatcatt gcgcagacca gatcctcaaa
    16621 cagcgctacc tgctgcggtt ttaagccgct ggcaccgagg aacgattttg gtgatttcac
    16681 aaaccacact tcttctggat catcaatgta ctgtgccagt gaggaaagac cgaactgcac
    16741 gcttttcgcc gtaacatcga tatcttcttc gcggttataa cgaatcgccc gacgtaacag
    16801 cgcctgcgtt tcatcgtcgt ctgccggaac atcatgatgg cggtacagcc attcgcttac
    16861 cgattcacgc gttggcgcgc aaagcattga aggcagcagc gtgctgtcgt tttccatttt
    16921 cagtaattgc ggtttaccgt cacgcatgac cgccactgaa cagtttgctg taccgtaatc
    16981 aaaaccaata aacacgaatt aatccccatg ccggtgaaga aggggcgtga ctttagcgaa
    17041 atgttgctgt cgcgacaacc ggaatatgaa agcaaagcgt aacgtctgaa taacgtttat
    17101 gctgaaagcg gattaacaag gagatgcgat gtataccctg aactggcagc cgccgtatga
    17161 ctggtcgtgg atgttgggat ttctcgccgc ccgtgcggtg agcggtgtgg aaacggtcgc
    17221 ggacaattat tatgcccgta gtctggcggt gggcgaatat cgcggcgtag tgactgctat
    17281 tccggatata gcccgccata ctctgcacat aaatttaagt gcaggtttag aacctgttgc
    17341 cgcagagtgt ctggcgaaaa tgagccgcct gcttgatctg caatgtaacc cgcagattgt
    17401 taacggtgcg ttgggaaagt taggcgcggg gcggcccgga ttgcgtttac ccggctgtat
    17461 tgatgctttt gagcagggtg tgcgggcgat tttaggccaa ctggtgagcg tggcgatggc
    17521 ggcaaaattg actgccaaag tggtacagct ttatggcgaa cggctggatg attttccgga
    17581 gtatatctgc ttccccacgc ctcagcggct ggcagcagcc gacccgcagg cattaaaagc
    17641 gttaggcatg ccgttgaaac gggcagaggc actgattcat ctggcaaatg cggcgctgga
    17701 gggcagctta ccaatgacaa ttccgggcga tgtggagcag gcgatgaaaa cgctgcaaac
    17761 ttttccgggt atcgggcgct ggacggcgaa ttattttgct ttgcgtggct ggcaggcgaa
    17821 agatgttttt ctgccggatg attatctgat taaacggcga tttccgggaa tgacaccggc
    17881 acaaatccgc cgttatgccg agcgctggaa gccctggcgt tcttatgcgc tgttgcatat
    17941 ctggtatacg gaaggctggc aaccagacga agcatgacgt tggtcgccgg atgcggtgcg
    18001 agcgccttat ccggtctggg gaatgagcga tttgtaggca tgataagacg cgttagcgtc
    18061 gcatcaggca atgggcaaac gctctctcag ttaatcgcga aataactgct attcaccagc
    18121 aaatccagcg gttgggcatc ggcaatcaca tcaccataaa tcagatcgac gccgatgcca
    18181 gaaagcgtat ccatcactaa gggtaaaacg actggcccgg cgatggtttt catcccgagt
    18241 cgctgagcgt gcccctgaat aatcgtaatc agcatctcat ccatcaaatt accctgcacg
    18301 ttggcgcata actcaccatc aagtagcagg taatctgcca tattcgcttt cagcgagttg
    18361 aagatttgca gatcgcggcc cacctggctg agcactatcc gacatcccgc cagctgcagt
    18421 ttttgcacgc tttctgcgtg atctaaaatc gcttcagccg gaataatcag atgtaataac
    18481 cgtggtggta gagggctatt ttccagctgc tcaagcagat cattcaccag cgtggcgcta
    18541 ctcaaaccgg caacggaaag ggggagggcg atgcttatgc ctttactggc aaccgctttg
    18601 gcggcctgct ggaaaaattc gtggaatacc cgtcggtcaa gagcatggct aagtgccgga
    18661 tcgctgaagc tacgacgaaa tgtttgttca tcaataattt cgccttcgca actccagagc
    18721 ttaagcgaaa tcagccacaa attacgcgct tccgggatcc gcggcgaagc gacaccgtgg
    18781 gcgatcatca tcaactgatt ctctttaatc atccgccact gttcatcaag cgacatcgcc
    18841 gcccgttcgc tatgtgcggc agcttgctgt ggttcgtaaa ccgtcacccg gccccgtcca
    18901 ccatttttgg aggcataaca ggcgatatca gcctgcgaca tcacttctgc cgcctgatga
    18961 ttgttgtcat cgatcaaggt aatcccggcg ctggcaccta cccgatgtac ccggccttcc
    19021 catataaagt gatagtcatt cacagcactg ataatacgtg tagcgataaa acgagcgctt
    19081 tcaacattgc agtctggcag cagcagacca aattcatcac cgccgagccg cgccagcacg
    19141 tcactggagc gcagcatact cagcatcaac gacgccagtt cgcgcagcaa agcgtcgccc
    19201 gccgcatgcc cggcgctgtc attcaccgct ttaaagcgat caagatcgat aaacaccagg
    19261 gcatgtcgct gatgcgtact gtttaccgtt tgcagcagga tacgcagttg tttctcaaaa
    19321 ctggcgcggt tggcgagatg cgtcagtgca tcatgggagg cgctgtagct cagctggcgc
    19381 agcattttgc gtgattcggt gacgtcctga atcaccagaa ccgaaccaat attgctgccg
    19441 tccagagtac ttaacggcgt aatactgtaa tgcacgtcgt aactgccgcc gctccgacag
    19501 tgcaacacca catcttgttc gattgcggaa cgtgaggtgt cggcactgta aatgttctcc
    19561 attaatggtc cgttgtcgcc aaaagtaata tgcaacaccg tcaggagcgg aacacctaac
    19621 gcttcttcct gcgtccagcc gctcatcttc tccgccactg gattcataaa ggtaattttc
    19681 atcgccatat caatacagac cacggcttca ccgatggaat caagcgtaat gtgcaggcgc
    19741 tctttttcct gaaacaatgc ctcgttaagc tgtttcactt cggtcatatc catattgatg
    19801 ccgaggagac gttcgacttc gccttcttta ttcagtaccc ggttggcgag ggcgcggata
    19861 tggcgaatac cgtcttttac ggtaatgcga aattccagtt taaagggcga gcgtgattgc
    19921 aacgaatcac gaatcacttt ttcggcatgc tggcgatctt ccggcagcac gcactcgtac
    19981 cacacctgcc agttcggttt gatatgtgga ggaatttcat acagctcgaa catccgctta
    20041 tcccagctaa aaacattcgg cttcaactcc ctctcccaga tgccaatccc gcccgcttcg
    20101 ttagccagcg tgatgcgctc catcagttgc tgattgacct gttcggtgcg ttttagctcg
    20161 ttaatgtctt caatctgagc gataaaatag agcggcgtgc catccgtgtg gcgcaccagt
    20221 gagacggcaa gcaacgccca gacaacatcg ccattgcggt tgtagtagcg tttttccatt
    20281 gaataggtgt ttatttcacc gcttatcagc ttttcaacct gttggagatc tttattgaga
    20341 tcctccggcc aggtcagttg ctgaaaggtg agtccgcgca gctcttcctg actgtacccg
    20401 agaaactggc agagcgcttt gttggtttgc agccattgtc cctcggtgcc cactaacgcc
    20461 ataccgatag cggaatattc catcgcgttc cgaaagcggg tttcgctttc ggaaatgtgt
    20521 ttgcgttccg cacgaaaggc atacatcacc atggtcatga tgttggcggg cagcaggatc
    20581 agcaaaaacg gtagccacgg catatggctc atcaggtacg tacgcggcgt agcaagcagg
    20641 gagggatccg cggccatcat cagcgacacc atcatcaccg tggtaaggaa gatcaaaaag
    20701 gcttccattc gtggcaggcg cacggcgctc cacatcaaca gcacaataat gaaagtaaaa
    20761 ggccacggca gatacagcat cgaaagccag cttaacgtca gtgtgatggc taacgtgagc
    20821 agcgactcaa aaagcaaccg tgggttgcga tggcgcagca gatagtgtgg tttaaataac
    20881 aatcccagcg gcaccagtgc cagagcgccg atggattctg acagtaccca tatcaaaaat
    20941 gccctgagag gatcgtctcc gggcgtcagc aggacaacca gaacaccccc caacagaggc
    21001 ggaacaatgg cgctgccgag tgccagacgc agccagtcag ccagattttg caaggggtta
    21061 taccacggca gcaatttacg tagcagcact gccccgacca cggcttcaac aatattgatg
    21121 gtcgtccagg tcatgttcag cgagctggtg gaaaaaagca ggatggatgc ggcgatattt
    21181 cccagcgaac agctcagcgc aattcccggc cacatgcgcc cggcatggcg ataaaacgcc
    21241 accatcatga tggacgtcgg gaaccacagt ggggcgagtt gggtgccaaa ctgcgaaagc
    21301 tcaagcgaga aaagggtaaa gataaacgag actaaaccta aactgaccag gtgaagcagc
    21361 gggtggggca gggcaattaa tacatgctgt gattgtttgc tcattaccgc tttatccgca
    21421 agaccaggtc gccagtattc gtttctgtgt ttatgctagt acaaaagttt tacattttat
    21481 atgcgaattg ctcataaagt gacgtaaagg cggataattt gcgcaactgc gtataacatt
    21541 ttttacctta cataaaactg atcaacgtaa tttgccaccg ggaattcttt gtcaacaatt
    21601 aactctgagc gcgcggcaac gctattcgac tggtatcaga cggatgaaat ccctataatt
    21661 gccgcgtttg gcgcttcgtc gcccccttcc taacatccag gttaatcagg tcgctaaata
    21721 tatgactgat cagtctcatc agtgcgtcat tatcggtatc gctggcgcat cggcttccgg
    21781 caagagtctt attgccagta ccctttatcg tgaattacgt gagcaagtcg gtgatgaaca
    21841 catcggcgta attcccgaag actgctatta caaagatcaa agccatctgt cgatggaaga
    21901 acgcgttaag accaactacg accatcccag cgcgatggat cacagcctgc tgcttgagca
    21961 tttacaagcg ttgaaacgcg gctcggcaat tgacctgccg gtttacagct atgttgaaca
    22021 tacgcgtatg aaagaaacgg tgacggttga gccgaagaag gtcatcattc tcgaaggcat
    22081 tttgttgctg acggatgcgc gtttgcgtga cgaacttaac ttctccattt tcgttgatac
    22141 cccgctggat atctgcctga tgcgccgcat caagcgtgac gttaacgagc gtgggcgttc
    22201 aatggattcc gtgatggcgc aatatcaaaa aaccgtgcgc ccgatgttcc tgcaattcat
    22261 tgagccttct aaacaatatg cggacattat cgtgccgcgc ggcgggaaaa accgcatcgc
    22321 gatcgatata ttgaaagcga aaataagtca gttctttgaa taagcttgat aaattgtgta
    22381 ccgttcagtg ataacctggt atgcccttga cgtaattggc gttaagggag tgatgcgcga
    22441 aaggagaaaa tgccatgcgt ctgtgtgacc gagatattga agcctggctt gatgaaggcc
    22501 gtttgtcgat caacccacgt ccgccagtgg agcgtattaa cggcgcgacg gtggatgtac
    22561 gcctgggcaa taaatttcgt accttccgtg gtcacacggc agcgtttatc gatctgagcg
    22621 gtcccaaaga tgaagtgagc gccgcgcttg accgcgtgat gagcgatgag atcgttctcg
    22681 acgagggcga ggcgttctat cttcacccag gagagctggc gctggcggtg acgctggagt
    22741 cggtgacgct gccagccgat ctggtgggct ggctggacgg gcgttcctct ttggcgcgtc
    22801 tggggctgat ggtgcacgtc accgcgcacc gtatcgatcc gggctggtct ggttgcattg
    22861 tgctggagtt ctacaactcc ggtaagctgc cgctggcgct gcgtccgggc atgttaattg
    22921 gtgcgctgag ctttgagccg ctttccggcc cggcggcgcg accttacaac cgccgtgaag
    22981 atgcgaaata tcgcaaccag cagggcgcgg tagccagccg aatcgataaa gactaattca
    23041 ggcccattga ggataccatg agacgatttc tgacgacgct gatgatactc ctggtcgtgc
    23101 tggtggccgg gttatctgtg ttagtgttgc tggtgaatcc gaatgatttc cgcgactata
    23161 tggtcaagca agttgctgca cgtagcggtt atcaattgca gctcgacggg ccactgcgtt
    23221 ggcacgtctg gccgcagctt agtatcctct ccgggcgaat gtctctcacc gcccagggcg
    23281 caagccagcc actggttcgc gccgacaaca tgcgtctgga cgtggcgctt ttaccactac
    23341 tgagtcatca actgagcgtt aagcaggtga tgctaaaagg ggcagtgatc caactgacgc
    23401 cgcagacgga agcggtgcgc agtgaagacg ctccggttgc accgcgcgac aataccttgc
    23461 cggatctgtc agacgatcgc ggatggtcgt ttgatatatc cagtcttaag gtggcggaca
    23521 gcgtgctggt gttccagcat gaagatgacg agcaggtgac aatccgcaat atccgcctgc
    23581 aaatggaaca agatccccaa catcgtggct catttgagtt ctccgggcgg gttaatcgcg
    23641 atcagcgcga tctcacaata tcccttaacg gtacggtgga tgcttctgat tatccgcatg
    23701 atttaacggc ggctattgaa caaattaact ggcagttgca gggtgccgat ttaccaaaac
    23761 aaggtattca ggggcagggg agtttccagg cccagtggca ggagtcacat aaacgccttt
    23821 catttaacca aatcagcttg accgccaatg acagtacgct gagcgggcaa gcacaggtca
    23881 cgctgacaga gaaaccggaa tggcagctga gactgcaatt cccgcaactg aatcttgaca
    23941 acctcatccc gcttaatgaa accgcgaatg gtgaaaacgg tgccgcgcag caggggcaga
    24001 gccaatcaac gttgccgcgc ccggtcattt cttcgcgtat tgatgaaccg gcctatcagg
    24061 gactgcaagg ctttacggct gatattttgt tgcaggccag taacgtgcgc tggcgcggaa
    24121 tgaattttac agatgttgcc acgcaaatga ccaacaagtc gggtttgctg gaaattactc
    24181 aactgcaggg caaacttaac ggtggacagg tttcactgcc gggcacgctg gacgcgacat
    24241 caataaatcc gcggataaac ttccagccac ggctggaaaa cgttgagatt ggtaccattc
    24301 tgaaggcgtt taactatccg atttcgttga ccggaaaaat gtcactggct ggtgatttct
    24361 ccggtgctga catagatgcc gacgcattcc gccacaactg gcaaggacag gcacatgtcg
    24421 aaatgaccga cacgcgcatg gaagggatga acttccagca gatgattcag caagcggtag
    24481 agcgtaatgg cggtgatgtg aaggccgctg aaaactttga taacgtgacg cggcttgacc
    24541 gctttaccac cgatttgacg ttgaaggatg gcgtcgtgac gttaaacgac atgcaaggtc
    24601 aatcgcctgt gctggcgctg acaggggaag gcatgttgaa tctggcagat caaacctgcg
    24661 atacccagtt tgatattcgg gtcgtgggtg gctggaacgg ggaaagtaaa ctgattgatt
    24721 tcctgaaaga aacgccagta ccgctgcggg tttatggcaa ctggcagcaa ctcaattaca
    24781 gcctgcaagt ggatcagtta ctgcgcaaac atctacagga cgaagcgaaa cgtcgcctga
    24841 atgactgggc cgagcggaat aaagattccc gcaatggcaa ggatgtgaag aagttgctgg
    24901 agaagatgta ggttaccagg tgaccagtcc tgtaggcctg ataagacgcg taaagcgtcg
    24961 catcaggcgt tcgtggcggc atcattatat tctgaatatg taagatcgaa ggctgttccg
    25021 ttcacttgat gttcctcgct actatggaac gctcaagccg gtgaacgtgc cagggtggct
    25081 caatcgccac caccctggca acccgggctc ccggcggtaa atcgccgctg gcgcggttcc
    25141 ctcatcttat tcctttcggc tattgggtaa aatgaccgat accatcacac ctcgtactcc
    25201 aactcgccat ccttatgcag cggaataatc tgcaccttct gcacgcgatg gctttccacc
    25261 tgcaacgttt taagcaaata atcccccacc tgaacttctt cgccaggttt tggaatacgc
    25321 tgtaaatatt ccatcaacag cccggcaatg gtgtgatatt cacgtttttc atccagcggc
    25381 agcggcacat attgcaccag atcttccagc ggcatatgac cattcgccgt ccaggaaccg
    25441 tccgcattct tctgaatatc atggcgggcg tcgatctctt ccacttcgtt cggtaagtta
    25501 ccgacaatgg tttcagtgac gtcacttaat gtcacaatcc cttccaccga gccaaactca
    25561 tccaccacaa aagcaaagtg cgtgcgggca ttacggaact gctccagggc aggtaacaac
    25621 ggcaaggttt ccgggaacac cagcggctgg cgaatcaaca cccgcaggtt gagcggttcg
    25681 ccgcgcagag actgttgtag caggtcgata acgtgaacaa caccgagcaa atcttctgcg
    25741 tcatcaccgt cggtaaccac cagccgcgta tgctgatttc gctccagcaa ctggcggatt
    25801 tcttcttccg gcgcgttgag atcgatatgc tcaatatcgt ggcgcgacgt cataatgctg
    25861 ctgacggtac gctggttaag attaagtacc cgctcaatca tccgccgttc ctgcggatta
    25921 aagatctgct ggttaccatg atccaccagc atggacgcgg tttcggcgtc cagttccgca
    25981 tcttcttttt gcccgctaag caggcgcatt accgcttcgg tagtccgctg gcgcagcgtc
    26041 tggttagcgg aaagaaaacg tcgccggtta aagatagcca actgattgag cgcctcgatc
    26101 atcaccgaga aaccaatggc agcgtacagg tagcctttcg gaatgacgaa gccgaaacct
    26161 tctgccacca ggctaaagcc aatcatcaac aggaagctta agcagagaat aacgatcgtc
    26221 gggtgactgt taacaaattg cgttaacggc ttgctggcca tcaacatcag gctgatagcg
    26281 ataaccacgg cggccatcat gaccagtaaa tggtcgacca tcccgacggc ggtaatcact
    26341 gagtcgagtg agaaaatggc gtccagtacc acaatttgcg tcaccacgcc ccagaacttc
    26401 gcacctttgc gttgtgtggg attattgctg tctttccctt ccagccgttc gttcagctcc
    26461 atcgtggctt tgaacaacag gaagaaacca ccaaacagca tgattaagtc gcgggcgcta
    26521 aaggtaaacg agcggaagct gaacagcggt tgagtcaggg tgaccagcca ggagattgac
    26581 gccagcagta acaggcgcat taacatcgcc agcagcagcc cggtaacccg tgcgcggtcg
    26641 cgctgcctcg gcggtagttt ttcggcgagg atggcaataa agaccaggtt atcaatgccg
    26701 aggaccagtt cgatcacaat cagcgtgatt aacccggccc agatagacgg atcggcaatc
    26761 cattccatag taagtacaaa acctattcgt tgtatgacaa atgtcacaat tcgatcatgg
    26821 ataaatcggg gcaaaaatgc aatgttgcag cactatttat ctcata
//
LOCUS       DAZOZD010000035        26499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOZD010000035 DAZOZD010000000
VERSION     DAZOZD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.035"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..324
                     /locus_tag="ACHUPB_001167"
     CDS             1..324
                     /locus_tag="ACHUPB_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0758044.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(427..1677)
                     /gene="icd"
                     /locus_tag="ACHUPB_001168"
     CDS             complement(427..1677)
                     /gene="icd"
                     /locus_tag="ACHUPB_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0758045.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1849..2502
                     /gene="rluE"
                     /locus_tag="ACHUPB_001169"
     CDS             1849..2502
                     /gene="rluE"
                     /locus_tag="ACHUPB_001169"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0758046.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2512..2973
                     /gene="nudJ"
                     /locus_tag="ACHUPB_001170"
     CDS             2512..2973
                     /gene="nudJ"
                     /locus_tag="ACHUPB_001170"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0758047.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3027..4133
                     /gene="mnmA"
                     /locus_tag="ACHUPB_001171"
                     /gene_synonym="trmU"
     CDS             3027..4133
                     /gene="mnmA"
                     /locus_tag="ACHUPB_001171"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0758048.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4169..4810
                     /gene="hflD"
                     /locus_tag="ACHUPB_001172"
     CDS             4169..4810
                     /gene="hflD"
                     /locus_tag="ACHUPB_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0758049.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4814..6184
                     /gene="purB"
                     /locus_tag="ACHUPB_001173"
     CDS             4814..6184
                     /gene="purB"
                     /locus_tag="ACHUPB_001173"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0758050.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6352..7023
                     /gene="phoP"
                     /locus_tag="ACHUPB_001174"
     CDS             6352..7023
                     /gene="phoP"
                     /locus_tag="ACHUPB_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0758051.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7023..8483
                     /gene="phoQ"
                     /locus_tag="ACHUPB_001175"
     CDS             7023..8483
                     /gene="phoQ"
                     /locus_tag="ACHUPB_001175"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0758052.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8559..9680
                     /gene="roxA"
                     /locus_tag="ACHUPB_001176"
                     /gene_synonym="ycfD"
     CDS             8559..9680
                     /gene="roxA"
                     /locus_tag="ACHUPB_001176"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0758053.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9729..10955)
                     /gene="pepT"
                     /locus_tag="ACHUPB_001177"
     CDS             complement(9729..10955)
                     /gene="pepT"
                     /locus_tag="ACHUPB_001177"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0758054.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11205..12341
                     /gene="potA"
                     /locus_tag="ACHUPB_001178"
     CDS             11205..12341
                     /gene="potA"
                     /locus_tag="ACHUPB_001178"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0758055.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12325..13182
                     /gene="potB"
                     /locus_tag="ACHUPB_001179"
     CDS             12325..13182
                     /gene="potB"
                     /locus_tag="ACHUPB_001179"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0758056.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13179..13973
                     /gene="potC"
                     /locus_tag="ACHUPB_001180"
     CDS             13179..13973
                     /gene="potC"
                     /locus_tag="ACHUPB_001180"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0758057.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13970..15016
                     /gene="potD"
                     /locus_tag="ACHUPB_001181"
     CDS             13970..15016
                     /gene="potD"
                     /locus_tag="ACHUPB_001181"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0758058.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15172..15993)
                     /gene="cobB"
                     /locus_tag="ACHUPB_001182"
     CDS             complement(15172..15993)
                     /gene="cobB"
                     /locus_tag="ACHUPB_001182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0758059.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16009..16920)
                     /gene="nagK"
                     /locus_tag="ACHUPB_001183"
     CDS             complement(16009..16920)
                     /gene="nagK"
                     /locus_tag="ACHUPB_001183"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0758060.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16949..18193)
                     /gene="lolE"
                     /locus_tag="ACHUPB_001184"
     CDS             complement(16949..18193)
                     /gene="lolE"
                     /locus_tag="ACHUPB_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0758061.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18193..18894)
                     /gene="lolD"
                     /locus_tag="ACHUPB_001185"
     CDS             complement(18193..18894)
                     /gene="lolD"
                     /locus_tag="ACHUPB_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0758062.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18887..20086)
                     /gene="lolC"
                     /locus_tag="ACHUPB_001186"
     CDS             complement(18887..20086)
                     /gene="lolC"
                     /locus_tag="ACHUPB_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0758063.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20348..21421
                     /locus_tag="ACHUPB_001187"
     CDS             20348..21421
                     /locus_tag="ACHUPB_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0758064.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21549..24995
                     /gene="mfd"
                     /locus_tag="ACHUPB_001188"
     CDS             21549..24995
                     /gene="mfd"
                     /locus_tag="ACHUPB_001188"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0758065.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25139..26095
                     /gene="ldtC"
                     /locus_tag="ACHUPB_001189"
                     /pseudo
     CDS             25139..26095
                     /gene="ldtC"
                     /locus_tag="ACHUPB_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26178..26435)
                     /gene="bhsA"
                     /locus_tag="ACHUPB_001190"
                     /gene_synonym="ycfR"
     CDS             complement(26178..26435)
                     /gene="bhsA"
                     /locus_tag="ACHUPB_001190"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR0758066.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6468 a         6874 c         6712 g         6445 t
ORIGIN      
        1 atgaagtggg tagtaattga cacggtaatt caacctacat gcggcatatc tttttcagcc
       61 atatggggtg atatgaaaaa gatcatctgg tatcaatcta ctatatttct tcctcctgga
      121 agtatattta caccggttaa gcttggtatt atccttaagg aaaaagaata tcctattact
      181 atttataaca ttgcaccatt caacaagaat ttatggagtt tactcaaaag cagtcaagag
      241 tgtcctccag gagaaagcaa aataacaaat aaatgtttac ataatagttg cattataaaa
      301 atatgcccat atgggctcaa gtaatgggct tcatataaat atgctcgata tgctattaat
      361 aatatccaca taaataaaac aacgggcgtg ttatacgccc gtttcaatat ttaactcatg
      421 gagagattac atgttttcga tgatcgcttc accaaactct gaacatttca gcagcttagc
      481 gccttccatc agacgttcaa agtcataagt tacggttttc gcgttgatag caccttccat
      541 acctttaaca attaaatcag ccgcttcagt ccaccccata tggcgcagca tcatctcagc
      601 ggagaggatg atagagcctg gatttacttt gtcctgaccg gcatatttcg gcgcagtacc
      661 gtgggtggct tcaaacaggg cgcattcgtc accgatgttt gcgccagggg cgataccgat
      721 accgccaacc tgcgctgcca gggcgtcaga aatgtagtca ccgttcaggt tcatacaggc
      781 gataacatca tattcagccg gacgcagcag gatctgttgc aggaatgcat cagcaatcac
      841 gtctttaatg acgatctctt tgccggtgtt cgggttttta actttcagcc acgggccgcc
      901 gtcgatcagt tcaccgccaa actcttcacg cgccagctgg tagccccagt ctttaaacgc
      961 gccttcggtg aacttcatga tgttgccttt gtgcaccaga gtcacagagt cacgatcgtt
     1021 agcaattgcg tattcgatcg ctgcacgaac cagacgtttg gtgccttctt ccgaacacgg
     1081 cttaataccg ataccacaat gttccgggaa gcgaattttc ttcaccccca tctcttcacg
     1141 caggaattta atcactttct cggcgtcggc agagtctgct ttccattcga tacccgcata
     1201 aatgtcttcc gagttttcac ggaagataac catatcggtc agttcagggt gtttaaccgg
     1261 gcttggagtg ccctgatagt aacgtaccgg acgcaggcag atgtagagat ccagttcctg
     1321 gcgcagggca acgttcagag agcgaatacc gccaccaaca ggagtggtca gcggaccttt
     1381 aatggcaacg cgatattcac gaatcagatc aagggtttca gcaggcagcc agacatcctg
     1441 accataaacc tgtgtggatt tttcaccggt gtaaatttcc atccaggaga ttttacgctc
     1501 gcctttatag gctttctcga ctgcagcgtc gaccactttc agcatggctg gggttacatc
     1561 tacaccgatt ccatcacctt caatgtaagg gataatcgga ttttcaggaa cgttgagttt
     1621 gccgttttgc agggtgatct tcttgccttg tgccggaaca actactttac tttccattca
     1681 cctctccttc gagcgctact ggtttgctcg tctgccacca cgttgcatat gcgtttgcgt
     1741 cctgcgatac ggatgcttta gagcaatttt ttgttaatga tttgtaattg gcttgtcaat
     1801 gctaccctat tgtttgccgt catgaaagat gcgcgttatt aggctataat gcggcaattc
     1861 ataatctctg aaaataccat gcaaaaaact tcttttagaa atcaccaggt taagcgattc
     1921 agctcgcaac gttctactag gcgtaaacct gaaaaccagc ccacgcgtgt gatcctgttc
     1981 aataaaccct acgatgttct tccgcagttc accgatgaag ccggacgcaa aacattaaaa
     2041 gaattcatcc cggttcaggg cgtttatgca gcaggtcgtc ttgaccgcga taccgaaggg
     2101 ttactggtgc tgaccaataa cggcgcgttg caggcgcgtt taacccagcc gggcaaacgc
     2161 accgggaaaa tctattatgt gcaggtggaa ggtattccca cacaagacgc acttgaagcc
     2221 ttgcgcaatg gcgtaacctt aaatgatggc cctaccctgc ccgccggcgc ggagctggtt
     2281 gacgaaccag cgtggttatg gccgcggaat ccaccaattc gtgaacgcaa aagtattccc
     2341 accagctggc tgaagatcac cttatatgaa ggacgtaatc gccaggtgcg ccgcatgacc
     2401 gcccatgttg gtttccccac gctgcgactg attcgttatg cgatgggtga ttacactttg
     2461 gataaccttg ccaatggcga atggcgagaa gcaacagaat aaggatgcac aatgtttaaa
     2521 ccgcacgtta ccgttgcttg cgtagtgcac gcagagggta aatttttagt cgttgaagag
     2581 acgattaatg gtaaagcgtt atggaaccaa cctgccgggc atctggaagc cgatgaaacc
     2641 ttagtggaag ctgccgcccg tgagctatgg gaagaaaccg gcatcagcgc gcaaccgcaa
     2701 cactttattc gtatgcatca gtggattgcg ccagataaaa cgccgttttt gcgtttcctc
     2761 tttgccattg agcttgagca aatatgcccg acccagcctc atgacagcga tatcgactgc
     2821 tgtcgttggg tcagcgccga agaaatttta aaggcgtcaa atcttcgttc gccgctggtg
     2881 gcggaaagta tccgttgtta tcaaagtggg caacgctatc cgctggagat gattggcgat
     2941 tttaactggc cttttacaaa gggtgtcatc taaaaggctg cgtgatagaa tacgccgcct
     3001 tgaagttcaa tgtcgtgagt gatccaatgt ctgaaaccgc aaaaaaagta atcgtcggca
     3061 tgtccggcgg tgtcgattcc tccgtttctg cctggctgtt gcaacaacag ggatatcagg
     3121 ttgaaggcct gtttatgaag aactgggaag aagacgacgg tgaggaatat tgcacagcgg
     3181 cagcggatct ggctgatgcc caggctgtct gcgacaagct cggcattgaa ctgcacaccg
     3241 ttaactttgc tgccgagtac tgggacaatg tcttcgaact gttccttgcc gaatataaag
     3301 ccggtcgcac gccgaatccg gatattctgt gcaacaaaga gatcaaattt aaagctttcc
     3361 tcgaatttgc cgccgaagat ttaggtgccg attatatcgc taccggtcat tacgtacgtc
     3421 gggccgatgt cgatggcaag agccgcctgc tgcgtggtct ggacagcaat aaagaccaga
     3481 gctacttcct ttatacgctc agccatgagc agattgcgca aagcctgttc ccggtcggcg
     3541 aactggaaaa accgcaggtg cgtaagattg ctgaagatct tggtctggtc accgcgaaga
     3601 aaaaagactc taccggcatt tgcttcattg gcgaacgtaa attccgcgag ttcctgggcc
     3661 gttatctccc ggggcaaccg ggcaaaatca ttaccgtcga tggcgatgaa attggtgagc
     3721 accaggggct gatgtatcac actctcggtc agcgtaaagg tctgggtatc ggtggcacca
     3781 aagaaggcac cgaagaacct tggtatgtgg tggacaaaga cgtcgaaaac aacattctgg
     3841 ttgtcgctca gggccatgaa cacccgcggc tgatgtctgt cgggttgatt gcccagcagt
     3901 tgcactgggt cgatcgcgaa ccattcaccg gcactatgcg ttgcacggta aaaacccgct
     3961 atcgccagac cgatatccct tgcaccgtca aggcgctgga cgatgatcgc attgaagtga
     4021 ttttcgatga accggttgcc gccgtgacgc cgggccagtc tgccgtcttc tataacggtg
     4081 aagtgtgcct cggtggcggt attattgagc agcgtctgcc gctgccggtc tgattattat
     4141 ctttacttaa aacaaggaag cagtgaacgt ggcaaagaat tactatgaca tcaccctcgc
     4201 cctggccggt atttgtcagt cggcacgcct ggtgcaacaa ctcgctcacc aggggcattg
     4261 tgatgccgat gcgctacacg tctcactcaa cagtattatt gatatgaacc ccagctcgac
     4321 gctggcggtt tttggcggta gcgaagccaa cctgcgcgtc gggctggaaa ccctgctcgg
     4381 cgtgctcaat gccagcagtc gccagggctt aaacgccgaa ttaacccgct acacactcag
     4441 cttgatggtg cttgagcgca aactctcctc agcgaaaggc gcgctcgaca ctctgggcaa
     4501 ccggatcaac ggcctgcaac gccagctcga acacttcgat ttacagtccg aaacgctgat
     4561 gagcgcgatg gctgctatct atgttgatgt gatcagcccg cttggcccac gcattcaggt
     4621 caccggttcc cctgctgtac tgcaaagccc acaagtgcag gcgaaagttc gcgcaaccct
     4681 gctggcaggc attcgcgccg ccgtgctctg gcaccaggtc ggcggcggac gtctgcaact
     4741 gatgttttct cgtaatcgcc tgaccactca ggcaaaacaa attcttgctc atttaacccc
     4801 ggagttgtga tctatggaat tatcctcact gaccgccgtt tcccctgtcg atggacgcta
     4861 cggcgataaa gtcagcgcgc tgcgcgggat tttcagcgaa tatggtttgc tgaaattccg
     4921 tgtacaagtt gaagtacgtt ggctgcaaaa actggccgcg cacgcagcga tcaaggaagt
     4981 tcctgctttt gctgccgacg caatcggtta ccttgatgca attgtcgcca atttcagcga
     5041 agaagatgcc gcacgcatca aaaccatcga gcgtaccact aaccacgacg ttaaagcggt
     5101 tgagtatttc ctgaaagaaa aagtggcgga gatcccggaa ctgcacgcgg tttctgaatt
     5161 catccacttt gcctgtactt cggaagatat caataacctc tcccacgcat taatgctgaa
     5221 aaccgcgcgt gatgaagtga tcctgccgta ctggcgtcaa ctgattgatg gcattaaaga
     5281 tctcgccgtt cagtaccgcg atatcccgct gctgtctcgt acccacggtc agccagccac
     5341 gccgtcaacc atcggtaaag agatggcaaa cgtcgcctac cgtatggagc gccagtaccg
     5401 ccagcttaac caggtggaga tcctcggcaa aatcaacggc gcggtcggta actataacgc
     5461 ccacatcgcc gcttacccgg aagttgactg gcatcagttc agcgaagagt tcgtcacctc
     5521 gctgggtatt caatggaacc cgtacactac ccagattgaa ccgcacgact acatcgccga
     5581 gctgtttgat tgcgttgcac gcttcaacac cattctgatc gactttgatc gtgacgtctg
     5641 gggttatatc gcccttaacc acttcaaaca gaaaaccatt gctggtgaga ttggttcttc
     5701 caccatgccg cataaagtta acccgatcga cttcgagaac tccgaaggga atctgggcct
     5761 ttccaacgcg gtattacagc atctggcaag caaactgccg gtttcccgct ggcagcgtga
     5821 cctgaccgac tccaccgtgc tgcgtaacct cggcgtgggt atcggttatg cgctgattgc
     5881 gtatcaatcc accctgaaag gcgtgagcaa actggaagtg aaccgtgacc atctgctgga
     5941 tgagctggat cacaactggg aagtgctggc tgagccaatc cagacagtta tgcgtcgcta
     6001 tggcatcgaa aaaccgtacg agaagctgaa agagctgact cgtggtaagc gcgttgacgc
     6061 cgaaggcatg aagcagttta tcgacggtct ggcgttgcca gaagaagaga aagcccgcct
     6121 gaaagcgatg acgccggcaa actacattgg tcgcgccatc accatggttg atgagctgaa
     6181 ataaactcgt atcagtgccg gatggcgatg ctgtccggcc tgcttattaa gattatccgc
     6241 tttttatttt ttcactttac ctcccctccc cgctggttta tttaatgttt acccccataa
     6301 ccacataatc gcgttacact attttaataa ttaagacagg gagaaataaa aatgcgcgta
     6361 ctggttgttg aagacaatgc gttgttacgt caccacctta aagttcagat tcaggatgct
     6421 ggtcatcagg tcgatgatgc agaagatgcc aaagaagccg attattatct caatgaacat
     6481 ttaccggata ttgcgattgt cgatctcgga ttgccagacg aggacggtct gtcactgatt
     6541 cgccgctggc gtagcaatga tgtttcactg ccgattctgg tattaaccgc ccgtgaaagc
     6601 tggcaggaca aagtcgaagt attaagtgct ggtgctgatg attacgtgac taaaccattt
     6661 catattgaag aggtgatggc gcgaatgcag gcattaatgc ggcgtaatag cggtctggct
     6721 tcacaggtca tttcgctacc accgtttcag gttgatctct ctcgccgtga attatctatt
     6781 aatgacgaag tgatcaaact gaccgcgttc gaatacacca ttatggaaac gttgatacgc
     6841 aataatggca aagtggtgag caaagattcg ttaatgctcc aactctatcc ggatgcggag
     6901 ctgcgggaaa gccataccat tgatgtactg atgggacgtc tgcgcaaaaa aattcaggca
     6961 caatatccgc aagaagtgat tactaccgtt cgcggccagg gctatttgtt cgaattgcgc
     7021 tgatgaaaaa attactgcgt ctttttttcc cgctctcgct gcgggtacgt tttctgttgg
     7081 caacggctgc ggtagtactg gtgctttcgc ttgcctacgg aatggtcgcg ctgatcggtt
     7141 atagcgtcag tttcgataaa actacgtttc ggctgttacg tggcgagagc aatctgttct
     7201 atacccttgc gaagtgggaa aacaataagt tgcatgtcga gttacccgaa aatatcgaca
     7261 agcaaagccc caccatgacg ctaatttatg atgagaacgg gcagctttta tgggcgcaac
     7321 gtgacgtgcc ctggctgatg aagatgatcc agcctgactg gctgaaatcg aatggttttc
     7381 atgaaattga agcggatgtt aacgatacca gcctcttgct gagtggagat cattcgatac
     7441 agcaacagtt gcaggaagtg cgggaagatg atgacgacgc ggagatgacc cactcggtgg
     7501 cagtaaacgt ctacccggca acatcgcgga tgccaaaatt aaccattgtg gtggtggata
     7561 ccattccggt ggagctaaaa agttcctata tggtctggag ctggtttatc tatgtgctct
     7621 cagccaatct gctgttagtg atcccgctgc tgtgggtcgc cgcctggtgg agtttacgcc
     7681 ccatcgaagc cctggcaaaa gaagtccgcg aactggaaga acataaccgc gaattgctca
     7741 atccagccac aacgcgagaa ctgaccagtc tggtacgaaa cctgaaccga ttgttaaaaa
     7801 gtgaacgcga acgttacgac aaataccgta cgacgctcac cgacctgacc catagtctga
     7861 aaacgccact ggcggtgctg caaagtacgc tgcgttctct gcgtagtgaa aagatgagcg
     7921 tcagtgatgc tgagccggta atgctggagc aaatcagccg catttcacag caaattggct
     7981 actacctgca tcgtgccagt atgcgcggcg ggacattgct cagccgcgag ctgcatccgg
     8041 tcgccccact gctggacaat ctcacctcag cgctgaacaa agtgtatcaa cgcaaagggg
     8101 tcaatatctc tctcgatatt tcgccagaga tcagctttgt cggtgagcag aacgattttg
     8161 tcgaggtgat gggcaacgtg ctggataatg cctgtaaata ttgcctcgag tttgtcgaaa
     8221 tttctgcaag gcaaaccgac gagcatctct atattgtggt cgaggatgat ggccccggta
     8281 ttccattaag caagcgagag gtcattttcg accgtggtca acgggttgat actttacgcc
     8341 ctgggcaagg tgtagggctg gcggtagccc gcgaaatcac cgagcaatat gagggtaaaa
     8401 tcgtcgccgg agagagcatg ctgggcggtg cgcggatgga ggtgattttt ggtcgccagc
     8461 attctgcgcc gaaagatgaa taaatatgtc catacttcac gcattacgtt aagcatccgt
     8521 tataatcggt tgcagatacc agcctgtgga tgcttaacat ggaataccaa ctcactctta
     8581 actggcccga ttttcttgaa cgtcactggc agaaacgccc ggtggtgtta aaacgcggct
     8641 ttaataattt tattgacccg atctctccag acgagttggc gggtctggcg atggaaagcg
     8701 aagttgacag tcgactggtc agtcaccagg atggcaaatg gcaggtcagc cacggcccgt
     8761 tcgaaagcta cgatcatctc ggtgaaacca actggtcatt actggtacag gcagtgaacc
     8821 actggcatga gccgaccgcc gcgctgatgc gaccgttccg tgaactaccg gactggcgta
     8881 ttgatgatct gatgatttct ttttctgtac ctggcggcgg cgtcggcccg catctcgatc
     8941 agtacgacgt gtttatcatt cagggtaccg gacgtcgtcg ctggcgagtg ggcgaaaagc
     9001 tgcaaatgaa acagcactgc ccacatccgg atctgttaca ggtcgatccg ttcgaagcca
     9061 tcatcgatga agagctggag cctggtgata ttctttatat tccgccagga ttcccgcatg
     9121 aaggctacgc gctggaaaat gcgatgaact attccgtggg ctttcgcgcg ccaaatacgc
     9181 gggaactgat tagtggattt gccgattatg tgctgcaacg tgaactgggc ggcaactact
     9241 acagcgatcc tgatgttcca cctcgcgctc atcctgcgga tgttctgcct caagagatgg
     9301 ataaactgcg tgagatgatg ctcgaattga tcaaccagcc ggaacacttt aagcaatggt
     9361 ttggcgagtt tatatcccag tcacgtcatg aactggatat cgcgccgcca gaaccgcctt
     9421 atcagccaga tgaaatctac gatgcgctga aacaaggtga tgtgctggtg cgcctgggtg
     9481 gtctgcgcgt attgcgcatt ggcgacgacg tgtatgccaa tggtgagaag atcgattccc
     9541 cgcaccgtcc ggcactggat gcactcgcca gcaacattgc gctgactgcg gagaattttg
     9601 gcgatgcgct ggaagatccg tcattcctcg cgatgctcgc ggcgctggtc aatagcgggt
     9661 attggttctt cgaagggtaa gtttggattt aagccggatg cggcatcaca tgccgcatcc
     9721 cttttcgctt acttccgttg cgccgttaac tcggcaatac ggacgatcac ctgcaccgct
     9781 ttttccatac cttccagagt cacaaactca tgcttaccat gatagttgta accgccagtg
     9841 aacaagttcg ggcacggtaa tcccataaac gacaactgcg cgccgtcggt accaccgcgg
     9901 atcggtttca gttccggttc aatatcgcag tcacgcatcg cctgctgggc gatatcgaga
     9961 atatgcggat gctcaaccac tttctctcgc atattgtagt aactgtcttc aatcaccagt
    10021 tcaatgtagc aatcaggatg taaccctttg cccacttttt tggcgatctc catcatttta
    10081 cgtttacgcg cttcaaactg tttacggtcg aaatcacgga tgatgtagtg catatcggcc
    10141 cgttcaacgg tgcctttcat gctcgccagg tgatagaaac cttcatagcc ttctgtcatt
    10201 tccgggcttt catccgccgg aacttccgca tgaatacgtg ccgccagcga cagcgcattt
    10261 accatcactc ctttcgccgt gcccggatga acattgttac cgacaatttt gatattgacc
    10321 gatgcggcgt tgaagttttc aaactccagt tcgcctacgc caccaccgtc aacagtgtaa
    10381 gcccagcggg catcgaaggc atcaacatca aaatgtttcg cccctttgcc cacttcttca
    10441 tccggggtaa aggcgacgcg aatatcacca tgcggaatgt ttttctgttg caataccgcc
    10501 agcgcggtca tgatttctgc aatacctgct ttgtcatcgg cacctaacaa ggttttacca
    10561 tcggtggtaa tcaacgtctg acccagtagc tgatgcagca ccgggaacat aaccggtgat
    10621 aaaacttcat cgccgatacc cagcgcaata tcgccaccgc gatagttttc aacaatttgc
    10681 ggattaacat ttttgccgct gcaatccggt gaggtatcca catgagaaat aaagccaatc
    10741 gccgggatat cgccagggac gttagccggt aacgtcgcca tcaaagtgcc cttctcactt
    10801 aaggtcacat tgataagccc catttcttcg agctgctctt tcagcagatg caataacttc
    10861 cattggcctt ccgtgctggg aacctgtctc acccctgctt ttgattgggt atccagagat
    10921 acgtagttca aaaatcgctc aagtagttta tccatgtagt caccctcact ttttgtgaca
    10981 acattattaa gaagcaagaa aagacaaata ttgcgtcagg tcacttttac ccctgcaagc
    11041 gggaatattt atcagcatta ccttaatgaa tataaagcta agccagtaat tcacaacaaa
    11101 gattggcgat tcaagcggtt tgccgtagaa ttaccgccct cattaagagt caccaaggtg
    11161 gttaaccaca aaccccgcat cggtaagcca tccgttgcgt ttacatggga cagagtaaaa
    11221 aattgaataa acaaccgagt tcgctttcac cgctggtgca attggcggga attcgcaaat
    11281 gctttgatgg taaagaggtc attccccagc tggatctgac tatcaacaat ggcgagttcc
    11341 tcacgctgct tggcccttct ggctgcggta aaacaaccgt tcttcgcctg attgcaggtc
    11401 tggaaactgt tgattccgga cgcatcatgc tggataacga ggacatcacc cacgttccgg
    11461 cggaaaaccg ctatgtgaac actgttttcc aaagctacgc acttttcccc cacatgaccg
    11521 tgttcgaaaa tgtggccttt gggttgcgca tgcaaaaaac ccccgctgct gaaattacgc
    11581 cccgcgtgat ggaagccctg cggatggtgc aactggaaac cttcgctcaa cgtaaaccgc
    11641 atcagctctc tggtggtcaa cagcaacgcg tcgccattgc tcgcgcggtg gttaacaagc
    11701 ctcgtctgtt gttgctggat gagtcgctct cagcgctgga ttacaaactg cgtaagcaaa
    11761 tgcagaacga gctgaaagcg ttacagcgta agcttggtat taccttcgtc tttgtcactc
    11821 acgatcagga agaagcgctc accatgtcag acaggattgt ggtgatgcgc gagggtcgta
    11881 ttgagcaaga tggtacaccg cgcgaaattt acgaagagcc gaaaaacctg tttgttgccg
    11941 gcttcattgg cgaaatcaat atgtttaacg ccactgtcat cgaacgtctc gacgagcagc
    12001 gcgtacgcgc caacgttgaa ggccgcgaat gtaatatcta cgttaacttc gccgttgaac
    12061 cggggcaaaa actgcatgtt ctgctgcgcc cggaagactt acgtgttgaa gagattaacg
    12121 acgacaacca cgctgaaggg ctgattggtt acgttcgtga gcgtaactac aaaggcatga
    12181 cgctggagtc ggttgttgaa ctggaaaatg gcaagatggt gatggtcagc gaattcttca
    12241 atgaagacga ccctgacttt gaccactctc tcgaccaaaa aatggccatt aattgggtag
    12301 aaagctggga ggtcgtactg gctgatgaag aacacaagta agttccagaa tgtagtgatt
    12361 gtcactattg tcggttggct tgtgttgttt gtctttctgc ccaacctgat gatcattggc
    12421 accagctttt tgacccgcga cgacgccagt ttcgtcaaaa tggtctttac gctggataac
    12481 tacacgcgtc tgctcgatcc gctctatttt gaagtgctat tgcactcgct gaatatggcg
    12541 ctgatcgcca ccctcgcctg cctggtgctg ggctacccgt ttgcctggtt tctggcgaag
    12601 ctgccacaca aggtgcgtcc gctgctgctg tttctgctga ttgttccctt ctggaccaac
    12661 tcactgattc gcatctacgg gctgaaaatt ttcctcagca ccaaaggcta tctcaacgag
    12721 tttttgctct ggctgggcgt tatcgacaca ccaattcgta tcatgttcac gcccagtgcg
    12781 gtgattatcg gtctggttta cattctgcta ccgtttatgg tgatgccgct gtactccagt
    12841 atcgaaaaac tggataagcc gttgcttgag gcggcgcgcg atctcggtgc cagcaagtta
    12901 cagactttta tccgtatcat tattccactg acgatgccgg gaattattgc cggatgtctg
    12961 ctggtgatgc tgccagcgat gggcctgttc tatgtatccg acctgatggg cggtgcgaaa
    13021 aacctgctga tcggtaacgt catcaaggtc cagttcctca atattcgtga ctggccgttt
    13081 ggtgcagcca ccagcattac gctgactatc gtaatgggcc tgatgttgct ggtttactgg
    13141 cgcgcttctc gtctgctgaa taaaaaggtg gaactcgaat gatcggtcga ctgcttcgcg
    13201 gcggttttat gaccgctatc tacgcgtacc tgtatatccc aatcattatt ttgattgtga
    13261 actcctttaa cagctcgcgc tttggcatca actggcaggg ttttaccacc aaatggtata
    13321 gcctgctgat gaacaacgac agcctgttac aggcagcgca gcattcacta acaatggcgg
    13381 tgttttcggc gacatttgct acgcttatcg gttcactgac ggcagttgcg ctgtaccgtt
    13441 atcgctttcg tggtaagccg ttcgttagcg gaatgctgtt tgtggtgatg atgtcaccag
    13501 atatcgtgat ggcgatttct ctgctggtgc tgtttatgct gctgggtatt cagcttggct
    13561 tctggtcgct gctgttctcg catatcacct tctgcctgcc gtttgtggtg gtgacggtgt
    13621 attcgcgcct gaaaggtttt gacgtgcgga tgctggaagc ggcgaaagat ctcggtgcca
    13681 gtgaatttac cattctgcga aaaatcattc tgccactggc aatgccagcg gtggcggcgg
    13741 gctgggtgtt aagctttacc ctgtcgatgg acgatgtggt agtttcttcg ttcgtcaccg
    13801 gaccaagtta tgaaattctg ccgttaaaaa tttattcgat ggtcaaagtc ggcgtatcgc
    13861 cggaagttaa cgcgctggca accatattac tggtgctgtc gctggtgatg gttattgcca
    13921 gccagcttat tgctcgtgat aaaacgaaag gtaacacagg ggacgttaaa tgaaaaaatg
    13981 gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc atgagcgccg ctcacgccga
    14041 tgacaacaac acgctgtatt tctacaactg gaccgagtac gtgccgccag gactgcttga
    14101 acagttcacc aaagaaaccg gtattaaggc tatctattcg acttacgagt cgaacgaaac
    14161 catgtacgcc aagctgaaaa cgtacaaaga cggtgcctat gacctggtgg ttccttcaac
    14221 ctattacgtc gataaaatgc gtaaagaagg gatgatccag aagatcgaca agtcgaagtt
    14281 aacgaacttc agcaatctcg atccagacat gctcaacaag ccgtttgacc cgaataacga
    14341 ctactccatt ccgtatatct ggggtgcgac ggcgattggc gttaacggtg atgcggtgga
    14401 tcctaaatct gtcaccagtt gggcagatct gtggaagcca gagtacaaag gtagcctgct
    14461 gctgaccgac gatgcccgtg aagtgttcca gatggcgctg cgcaaactgg gctactccgg
    14521 taacaccacc gatccgaaag agattgaagc tgcatataac gagctgaaaa aactgatgcc
    14581 aaacgtcgca gcgtttaact ccgataaccc ggctaacccg tacatggaag gcgaagttaa
    14641 cctcggcatg atctggaacg gctctgcttt cgttgcacgc caggcgggta cgccaattga
    14701 cgtggtgtgg ccgaaagaag gcggcatttt ctggatggac agcctggcga tcccggcaaa
    14761 tgccaaaaac aaagaaggcg cgctgaaatt gatcaacttc ctgctgcgcc cggatgtggc
    14821 aaaacaggtt gccgaaacta tcggttatcc aacgccaaac cttgcggcgc gtaagctgtt
    14881 aagcccggaa gtggcgaacg ataaaacgct ctacccggat gctgaaacca ttaaaaatgg
    14941 tgaatggcag aatgacgttg gcgcagccag cagcatttat gaagagtatt atcagaagct
    15001 gaaagcagga cgttaattca gcaaacgggc ggtggtatta ccgcccgttt tttagttagc
    15061 aaggatgtgg ctaatgagtc aggacagtaa agttttatcc cgctctttcg tcggtattgt
    15121 ggtccccttg taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc
    15181 cttcagcaac ttttcaacaa actcaggcac cacctggctt gccgggccgt aatatttctc
    15241 ggcaaattca ttaccgacct gacttggttc gagattcagt tccacggtgt gcgcgccatg
    15301 cagtttcgct tcgtgaacaa acccagccgc cggataaaca tgcccggaag taccaatggc
    15361 aatgaaaata tcagccatcg acaacgccat ataaatttca tccatgccga gtggcatttc
    15421 gccaaaccaa acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc
    15481 ttctggcgta acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt
    15541 cagcaattcc ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc
    15601 tatattctgc gttaccagca aaaagcgatc gccgagggcg tcttgcagtt tagccagcgc
    15661 aagatgcgcg gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata
    15721 aaacgcttgc accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac
    15781 ccgatgttct tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga
    15841 aattcctgcc cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt
    15901 atctctgaaa aaaatacgct gacgcaaacg ctcgcgcagg cggcgtttat ttttacgaaa
    15961 acgacttaac cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag
    16021 atgtaggaag gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg
    16081 cggaacacga gctacaggta agagatgacg aggcagcctg tccgccagtt gcgttgtgat
    16141 tgccgggaaa ttcgataagc caccaccaat gacgaccagg tcagagtcaa cgatggtcag
    16201 gatatttccc agacaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg
    16261 ctcatcgcct tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata
    16321 gtgttgccag agccacgcaa agccccgacc tgatagataa ttttcgatac aaccaatttg
    16381 accacagccg cagcggcgta acgggaaatc cagccccatc atggttaacg catcaaccgg
    16441 cagacgcata tggccaaact cgccggtaat gtagctttta ccggtaatcg gtttaccgtt
    16501 gaaaatcagc ccgccgccaa cgccggtgcc gagaattaac cccatcacca gtggatattg
    16561 cgtaaattca tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg
    16621 tacatcgcga tcaagacgtg cgctcaggtc ggcacgcagc ggtttaccgc tggcagcggg
    16681 gacattggcg gcatacagcg taccatcttc tgtttccggc attcccggaa taccgatgcc
    16741 aacagaacct ttacagccaa aacgttggtc agcttcggct accagctcgc atactgcatc
    16801 taaaaatgcg tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg
    16861 accgctatca aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat
    16921 tgccgctcct tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa
    16981 tattactggc gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga
    17041 ccagtacgta gaagacgtcc agccagtgca attccgatgg caggaagtca ataaaataga
    17101 tatcgctgga gaggaactga tgaccgatca gcttttcaat ccgctcaata atcggggtaa
    17161 gttgcagtga aacaactacg ccgataatca caccacacag gctaccgaac agccctgcca
    17221 gcaatccata ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta
    17281 atactgcgat atcgccactc ttgtctttca ccgccatcac taaggtggag acgatgttga
    17341 aacaggccac gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga
    17401 tatcgcgata catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg
    17461 tcacttcacc cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg
    17521 caatacctga cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca
    17581 tggcaaaact gtgatcgagt tgaccactca actgcaaaat accggcaatg tgcaaacgca
    17641 cacgttttgg ctgcatcagt ttatgctcgg gattcgagtt gggaatcatg atcgacaccc
    17701 aatcgccctg cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt
    17761 cgcccgcttt aaaattgcgc caggcatccc cctgaacaaa cgagggtaat gcgctcagac
    17821 gctgttcctg ttgcgggtta acgcccttca cctgaattgc gcgcagattg gtaccacttt
    17881 ctaccagtcc ggtgaaattg atatacggcg cagcggcggc aatacccggc actttttgca
    17941 cgttatccag tgcttcctgc cagttagtcc atggctgatt taccgcctca atttcaccgt
    18001 gcggtaccac agcaagaatg cggttattca gttcgcgttc aaagccgttc atcgcgctta
    18061 agccgacgat caataccgcc acgccgaggg caatgccaat ggtagaaatg acagagatta
    18121 gcgacaccat gccgccgcgt cgccgtccgc gactaaaacg caggccaatc aataacgata
    18181 aaggcatcgc cattactccg cccccatcag gctcagttcc gccgtcagac gtccatcacg
    18241 catttccagt tggcggctca tacgtttcgc cagttgcagg tcgtgagtaa ccaccaggaa
    18301 ggcggtgccc tgcaagcgat tcaattcccc aagcaactgg aagatgctgt cggcgttacg
    18361 cgcatcgagg ttaccggtag gctcatccgc aagtaccaga cgcggattat tgaccagcgc
    18421 acgggcaatc gccacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc
    18481 acgatgctcc agccccaccg cttttaacat ctccagcgca cggctgttga tttcagcggg
    18541 ctttttcttg ccaatcagca gtggcatagc cacgttttcc agggcagtaa aatccggcag
    18601 caggtggtga aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc
    18661 cgaagacagt ttgctcatcg gttgaccgtt aaagatcaca tcgccggagg ttggtgtatc
    18721 cagcccgccc agcaggtgca gcaaggtact tttaccggaa ccagagctac cgacgatcgc
    18781 catcatttca ccttcgccga cgctgaaact gacattgtgc agtacatcgg tttgcacact
    18841 gccttcctga tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta
    18901 aagcctcagc gggttgagtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg
    18961 cgatagccat cgccaccagc gcaataacaa tgacctgtaa aggttcgata gcgaccggca
    19021 gcgccgcgcc atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa
    19081 gcagtgcgcc aagcgccgct ccgaggatcg caccgataat cccggcgctg gccccttgca
    19141 ccataaagac catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt
    19201 cgccctgctt ctccattacc atcagcccta gtgaggtaat aatgttaaac gccgcaacgg
    19261 cgacaatcag gctcagcagc aagcccatca tatttttttc catgcgtacg gcctggaaca
    19321 gctcgccttt acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac
    19381 ttaaagagtc aactttcagc ggctcatcca gccacaaacg ccagccggta atattgcctg
    19441 ccggataacg catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga
    19501 cttcactgtt ggcggcgaaa ttgccaatca cattgaacag gcgctggctt ggaatacgcc
    19561 ccatcggcgt gaactggctg gcagatggta ccatcacgcg gatttgatca ccgcgattaa
    19621 cgcctagctg tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt
    19681 cagtttgttt cacattgacc agatacggcg taagtggatc tttttgcgcc gggtcgatac
    19741 cgagcatcac cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa
    19801 taggtgcgac gcgattaacg ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt
    19861 taagagagcc atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt
    19921 gcagctcgcg ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga
    19981 gggtaatgcc gatggtagaa agccaggaga cgaaacgacc gaagcgatcc gctgcacgcc
    20041 cacgcatgta acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt
    20101 tgctgtagca aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta
    20161 agtgatcgtc agggctaatc tgaaaagccc ccgtaaatca atcggctaaa ttgccatgtt
    20221 ttatcatcaa tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc
    20281 cattacacct cgggtcataa cccgcaggat caaccgcatg taaattaaaa aagagcgtgt
    20341 tgtcctgatg aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct
    20401 ggtggtgatt tatcactcgg tcattacctt ttatccgcat atgaccactt ttcagcatcc
    20461 gttatcggaa gtcctgagta aatgctggat ctatttcaat ctttaccttg caccctttcg
    20521 tatgccggtt tttttcttta tatccggcta tttgattcgc cgctatatcg acagcgtacc
    20581 gtggggaaat tgtctcgata aacggatctg gaacatcttc tgggtgctgg cgctttgggg
    20641 cgtggtgcag tggctggcgt taagtgcgct gaaccagtgg ctggcacctg agcgcgattt
    20701 aagcaatgcc tccaatgccg cttatgccga ttccaccggt gagttcctgc acgggatgat
    20761 caccgccagc accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa
    20821 aatttttagc cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt
    20881 taatttcgtt cccacgccgt ggtggggaat gaacagtgtg atccgcaatt tgccttatta
    20941 cagccttggc gcatggtttg gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg
    21001 ccgccatctg ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac
    21061 tatctcgctg ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcagta
    21121 cgagcaacga ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat
    21181 tgctatctac accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca
    21241 aatgaacgca gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg tttatccctt
    21301 tgtcagtttg ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgtgc
    21361 attcagcgat ctgttgttct ccccgccttc actgcccgct gccgtcagtt actctcgtta
    21421 aaccatcgcc ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa
    21481 cagcattgct tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga
    21541 caaccgttat gcctgaacaa tatcgttata cactgcccgt caaagcgggt gagcagcgtc
    21601 tgctgggcga gttaaccggc gcagcctgtg caacgctggt agcggaaatt gccgaacgtc
    21661 acgccggtcc ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg
    21721 aaatcagcca gttcaccgat cagatggtga tgaatctggc ggactgggaa actcttccct
    21781 acgacagttt ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc
    21841 taccgacgat gcagcgtggc gtactgattg ttccggtgaa tacgcttatg cagcgcgttt
    21901 gcccacacag ttttctccac ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac
    21961 gagatgcatt acgaacccaa ctggacagcg ccggttatcg ccatgttgac caggtgatgg
    22021 agcacggcga atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc
    22081 tgccttatcg tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg
    22141 acagccagcg cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc
    22201 cgaccgataa agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga
    22261 agcgcgatcc ggaacatatt taccagcaag tgagtaaagg cacattacct gccgggatcg
    22321 agtactggca gccattgttc ttcagcgaac cactgccgcc gctgttcagt tatttccctg
    22381 ccaatacctt gctcgtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg
    22441 acacgctggc gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac
    22501 aatcgctctg gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgggtgc
    22561 agctaaaaac tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac
    22621 tgccagacct ggccgttcag gcacaacaaa aagcgccgct ggatgcgctg cgtaagttcc
    22681 tcgagacttt cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc
    22741 tgggtgaact gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag
    22801 ccagcgaccg tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg
    22861 tgcgtaatct ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc
    22921 gtcaggattc tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc
    22981 atattggtca gccggtggtc catctggagc acggtgtcgg gcgctatgcc ggaatgacca
    23041 cgctcgaagc gggcggcatt actggcgagt atttgatgct cacctatgcc aacgacgcca
    23101 aactgtatgt tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag
    23161 aaaacgcccc gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg
    23221 cggaaaaagt gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca
    23281 aagagggctt cgcgtttaaa cacgatcgtg agcagtatca gttgttctgc gacagcttcc
    23341 cgtttgaaac cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc
    23401 agccgctggc aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg
    23461 cgatgcgcgc cgctttcctg gcagtagata accacaagca ggtggcggtg ttggtgccta
    23521 ccacccttct cgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg
    23581 tacgtatcga aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg
    23641 aagtggcgga agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg
    23701 tcaagtttaa agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc
    23761 ataaagagcg cattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc
    23821 cgatcccacg cacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca
    23881 cgccgcccgc ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctagtgg
    23941 tccgggaggc gatcctgcgt gaaattttgc gcggggggca ggtttattat ctctacaatg
    24001 atgtggaaaa catccagaaa gctgccgaac ggctggcaga actggtgcca gaagcgcgga
    24061 tcgccatcgg tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc
    24121 atcatcaacg tttcaacgtg ctggtttgta caaccattat cgaaaccggg atcgacatcc
    24181 cgacagccaa caccattatc attgaacgcg cggatcactt cggtctggcg cagctgcacc
    24241 agttacgcgg tcgcgtcgga cgttcgcatc atcaggcata tgcatggttg ctgacaccgc
    24301 atccaaaagc gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag
    24361 atctcggggc aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac
    24421 tgcttggcga agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt
    24481 tgctggaaaa cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca
    24541 ccagccagca aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcattc
    24601 ctgacgtgaa tacgcgtttg tcgttctata aacgtattgc cagcgccaaa acggaaaacg
    24661 aactggaaga gatcaaagtc gagcttatcg atcgcttcgg cctgctgcca gatccggcgc
    24721 gtaccctgct ggatattgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc
    24781 tggaaggtaa tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg
    24841 cctggttgat tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc
    24901 gcctgaaatt tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt
    24961 ttatgcgtga actggaagag aacgcgatcg cttaatggta aatcgggggc gtttctgcgc
    25021 ccccatctgc aacatttaca aattctttgc acttccctgc actatccgac actgtcacca
    25081 tccataattc aggcttatct gtttattaca ataaccttat atttattatt gatttttggt
    25141 gatgatcaaa acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc
    25201 gctaccggca aaagccaaca cctagccgct gccgccagcg agcagtcgtc tggttggcga
    25261 aaacaaattt catgtggtgg aaaatgacgg tggttctctg gaagccatcg ccaaaaaata
    25321 taacgtcggc tttctcgctc tgttacaggc taaccccggc gttgatcctt acgtaccgcg
    25381 cgcgggcagc gtgttaacga tcccgttgca aaccctactt ccagacgcgc cgcgcgaagg
    25441 cattgtgatc aacattgcgg agctgcgtct ctattactac ccgccgggta aaaattcggt
    25501 aaccgtgtat ccaataggta ttggtcagtt aggtggtgac acgctgacac cgacaatggt
    25561 gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa acatccgcgc
    25621 ccgttataaa gcacagggaa ttgagttgcc tacggtagtg ccggctggac cggataaccc
    25681 aatgggccat catgcgattc gtctggcggc ctatggcggc gtttatttgc ttcacggtac
    25741 gaacgccgat ttcggcattg gcatgcgggt aagttctggc tgtattcgtc tgcgggatga
    25801 cgatatcaaa acactcttta gccaggtcac cccaggcacc aaagtgaata tcatcaacac
    25861 tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac atcagccgct
    25921 gtcagagaag attgatgacg atccgcagct gctgccaatt acgctgaata gcgcaatgca
    25981 atcatttaaa gatgcagcac aaactgacgc tgaagtgatg caacatgtga tggatgtccg
    26041 ttccgggatg ccggtggatg tccgccgtca tcaagtgagc ccacaaactc tgtaaatcct
    26101 ggataaaaaa aatccccgcg acacggcatg tcgcggggaa aaaatcagca gtagcaggca
    26161 ttaatgaggg ttaatgctta tttataaatt actgctgttc catggagggt attcggaccg
    26221 gttacagaag taatacggaa agattttgcg cccatctcat ccgctttttg cgccagctgc
    26281 tcttccagcg atcccagatt tgtccccgcg ttagcactga tagtaccgac tttttgttgg
    26341 ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg caaatgacat ggagcttaaa
    26401 atcgccgcag cgatgagggt ttttacgttt ttcataatag tggccttatg cagatgaatg
    26461 acgtcgcaag aaattactta acgatcgata actaaatga
//
LOCUS       DAZOZD010000036        25758 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOZD010000036 DAZOZD010000000
VERSION     DAZOZD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25758
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.036"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            129..1604
                     /gene="xylE"
                     /locus_tag="ACHUPB_001191"
     CDS             129..1604
                     /gene="xylE"
                     /locus_tag="ACHUPB_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0758067.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(1648..2058)
                     /gene="psiE"
                     /locus_tag="ACHUPB_001192"
     CDS             complement(1648..2058)
                     /gene="psiE"
                     /locus_tag="ACHUPB_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0758068.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(2184..2327)
                     /locus_tag="ACHUPB_001193"
     CDS             complement(2184..2327)
                     /locus_tag="ACHUPB_001193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758069.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            2328..2606
                     /locus_tag="ACHUPB_001194"
     CDS             2328..2606
                     /locus_tag="ACHUPB_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758070.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(2653..4749)
                     /locus_tag="ACHUPB_001195"
     CDS             complement(2653..4749)
                     /locus_tag="ACHUPB_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0758071.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4749..5486)
                     /locus_tag="ACHUPB_001196"
     CDS             complement(4749..5486)
                     /locus_tag="ACHUPB_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0758072.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(5483..6121)
                     /locus_tag="ACHUPB_001197"
     CDS             complement(5483..6121)
                     /locus_tag="ACHUPB_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0758073.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(6235..6477)
                     /gene="yjbE"
                     /locus_tag="ACHUPB_001198"
     CDS             complement(6235..6477)
                     /gene="yjbE"
                     /locus_tag="ACHUPB_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0758074.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6939..8588)
                     /gene="pgi"
                     /locus_tag="ACHUPB_001199"
     CDS             complement(6939..8588)
                     /gene="pgi"
                     /locus_tag="ACHUPB_001199"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0758075.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      8825..9021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            9113..10462
                     /gene="lysC"
                     /locus_tag="ACHUPB_001200"
     CDS             9113..10462
                     /gene="lysC"
                     /locus_tag="ACHUPB_001200"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0758076.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            10714..10986
                     /locus_tag="ACHUPB_001201"
     CDS             10714..10986
                     /locus_tag="ACHUPB_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0758077.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(11119..11991)
                     /gene="rluF"
                     /locus_tag="ACHUPB_001202"
     CDS             complement(11119..11991)
                     /gene="rluF"
                     /locus_tag="ACHUPB_001202"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0758078.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            12339..13176
                     /locus_tag="ACHUPB_001203"
                     /pseudo
     CDS             12339..13176
                     /locus_tag="ACHUPB_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            13246..14052
                     /locus_tag="ACHUPB_001204"
     CDS             13246..14052
                     /locus_tag="ACHUPB_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0758079.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            14062..14469
                     /locus_tag="ACHUPB_001205"
     CDS             14062..14469
                     /locus_tag="ACHUPB_001205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0758080.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            14469..14963
                     /locus_tag="ACHUPB_001206"
                     /pseudo
     CDS             14469..14963
                     /locus_tag="ACHUPB_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            15029..15826
                     /locus_tag="ACHUPB_001207"
     CDS             15029..15826
                     /locus_tag="ACHUPB_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0758081.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15837..16661
                     /locus_tag="ACHUPB_001208"
     CDS             15837..16661
                     /locus_tag="ACHUPB_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR0758082.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            16713..17941
                     /gene="sorE"
                     /locus_tag="ACHUPB_001209"
                     /pseudo
     CDS             16713..17941
                     /gene="sorE"
                     /locus_tag="ACHUPB_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            18324..19014
                     /gene="pepE"
                     /locus_tag="ACHUPB_001210"
                     /pseudo
     CDS             18324..19014
                     /gene="pepE"
                     /locus_tag="ACHUPB_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(19105..20736)
                     /locus_tag="ACHUPB_001211"
     CDS             complement(19105..20736)
                     /locus_tag="ACHUPB_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0758083.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(20956..24639)
                     /gene="metH"
                     /locus_tag="ACHUPB_001212"
     CDS             complement(20956..24639)
                     /gene="metH"
                     /locus_tag="ACHUPB_001212"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0758084.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            24839..25663
                     /gene="iclR"
                     /locus_tag="ACHUPB_001213"
     CDS             24839..25663
                     /gene="iclR"
                     /locus_tag="ACHUPB_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0758085.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         6048 a         6768 c         6692 g         6250 t
ORIGIN      
        1 atcacaatta agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc
       61 cttaactgcc tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc
      121 aggtctgaat gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta
      181 cattaggtgg tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac
      241 tcaataccgt ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag
      301 gattttgcgt agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt
      361 gcagtaaccg cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt
      421 ctggtgtagg ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag
      481 tacctattta tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta
      541 ttggcgttgg tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc
      601 atattcgcgg aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag
      661 tttactgcgt aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg
      721 gctggcgtta tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt
      781 ataccgtgcc agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa
      841 gtatcctgcg caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac
      901 actccctgga tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga
      961 ttgtcatcgg cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt
     1021 actacgcgcc ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga
     1081 ccattattgt cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata
     1141 aatttggtcg taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta
     1201 gcctcggtac cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt
     1261 tctatgttgc tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa
     1321 tcttcccgaa tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg
     1381 cgaactactt cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc
     1441 atttccacaa cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat
     1501 ttatgtggaa atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct
     1561 gggaaccgga aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac
     1621 gccgcgccag ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag
     1681 ccacagagtg atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga
     1741 tttatgatcg acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg
     1801 tagcgggaag tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata
     1861 gagaaagtaa accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa
     1921 cagcacatct gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag
     1981 gcacagcagg ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg
     2041 acgagagagt gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca
     2101 aaaatgtgac ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc
     2161 gcctccacca aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca
     2221 tccggcaaga caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc
     2281 cagccaccag caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc
     2341 tgataaaggt ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc
     2401 ttcctgctta tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg
     2461 tctccagccc aatgatggcc cagtcgctca ggcaatcaga cctgcaaccg atatcgacgg
     2521 acaggaagac agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg
     2581 ttcctgatgg ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac
     2641 ctttcccatg acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa
     2701 cttacgccca agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg
     2761 gctgcgtgtc gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt
     2821 gaagtcccct tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac
     2881 caccacgccg ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag
     2941 atactgcccg acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc
     3001 ggtcagatgt ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg
     3061 ccagtcgcgc tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg
     3121 acgatacaac acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac
     3181 ctgaccgtag aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata
     3241 ggcatcgttc tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg
     3301 tggcggattg gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc
     3361 gatggtcagc agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata
     3421 catgtaaaag ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc
     3481 aaagcgtgat ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac
     3541 gcgtttttgc gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag
     3601 gctggcgaca tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg
     3661 gatcgttttg atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc
     3721 ttcgcgcgaa tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc
     3781 ctggatctgc ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac
     3841 caccgagtgc tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc
     3901 gatgtaggac gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt
     3961 gttgccacgt tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc
     4021 gacgttaaac ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc
     4081 ttcatactcc agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa
     4141 cagtgaggcc gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct
     4201 gttatcgcga tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct
     4261 ggtgcccaga tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct
     4321 ggcaacaaga tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc
     4381 cacttgcggc agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc
     4441 tttatacgtt tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc
     4501 ggtgtaacgc agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg
     4561 gtactgatcg ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt
     4621 gggagtttgt aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg
     4681 atatgtttcg ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct
     4741 ttttttcatt attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca
     4801 tcaggcgtct cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc
     4861 ggctctacgt gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg
     4921 taaacgaccc acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag
     4981 ctcgccacgt cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac
     5041 aacgtgaccg tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg
     5101 ttaccgcgtt cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc
     5161 acttccaatg cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg
     5221 gaatctgcgc cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc
     5281 gccgttgcca gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt
     5341 tgccctacca gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca
     5401 ttgctgaaca ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc
     5461 aacgcgacaa tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg
     5521 ggaaataatc cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc
     5581 agtagcggtt atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca
     5641 gggtttcatc gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg
     5701 cggtggcgta gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac
     5761 catcaacaat ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca
     5821 ggttgtcgcc gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt
     5881 gatcctgagt gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt
     5941 gcggcccgcc attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt
     6001 ccgtcagctg tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt
     6061 tagtcgtggc tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca
     6121 cgactcttct ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc
     6181 atatttcaag ccacagatgc aatgactcga attattttgg ggatacatac tttattactg
     6241 ggtactggtg gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc
     6301 cgcgagcgcg acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc
     6361 cgccgaaacc gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca
     6421 cacagaagtc gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac
     6481 aatttccctt cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta
     6541 tacacaacta aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat
     6601 tggaatgaat atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac
     6661 ttaaaataaa aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa
     6721 tctattttag gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat
     6781 ctgataaaac aatgccgact tattgtcggc attgttttta aggttattta actcctcata
     6841 ctatgcatta atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct
     6901 tatccggcct acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag
     6961 cggttaatca gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc
     7021 tctggcagaa tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta
     7081 aagatgttca ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca
     7141 cccaggctga acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg
     7201 aacactttga acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat
     7261 tcctgctcaa ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag
     7321 ttagacagca gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg
     7381 aaatcgcacg gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg
     7441 ttagtgcctg gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta
     7501 cggtcaacat acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg
     7561 tgcatatact ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac
     7621 cagatgccaa tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa
     7681 tgcttgtcca tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag
     7741 agaacaatcg acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag
     7801 aactcgaaca tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa
     7861 agcgccgcaa agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca
     7921 cgcgcgctat gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg
     7981 aacagcgtgg tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg
     8041 ttagaaacaa agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg
     8101 tacgggccga ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg
     8161 gtataaccct tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc
     8221 accgcgttga cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg
     8281 ttacgcagcg ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag
     8341 aacatcgact taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc
     8401 agcgtctctt cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt
     8461 gcggagaact tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct
     8521 ttcatttcat cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg
     8581 tttttcatta gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc
     8641 agtcacagaa aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta
     8701 gcgcagtgaa gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca
     8761 tgaccccgtc gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct
     8821 agcgcaggcc agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga
     8881 taacacctga gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc
     8941 tacaggggct gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag
     9001 tggtggagca cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc
     9061 cgctcttccc ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga
     9121 aattgttgtc tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag
     9181 cgctgatatt gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg
     9241 tatcactaat ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa
     9301 actcgacgct attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt
     9361 tatccgtgaa gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc
     9421 gctggcaacg tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac
     9481 cctgctgttt gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg
     9541 taaagtgatg cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc
     9601 ggaactggcc gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg
     9661 atttatcggt agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta
     9721 tacggcagcc ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt
     9781 cccgggcatc tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat
     9841 cgcgtttgcc gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac
     9901 gttgctaccc gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg
     9961 cgcaggtggt acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc
    10021 gcttcgtcgc aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg
    10081 tttcctcgcg gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac
    10141 cacgtcagaa gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga
    10201 tacgttgctg acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga
    10261 agaaggtctg gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg
    10321 caaagaggta ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc
    10381 cagccataac ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact
    10441 gcatagtaat ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt
    10501 gattttcttg tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt
    10561 atcgagaata attgccttta atttttttat ctgcatctct aattaattat cgaaagagat
    10621 aaatagttaa gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta
    10681 cactgaactc tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa
    10741 gagatgactg aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg
    10801 catggtcgcg tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa
    10861 ttaatggtcg agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct
    10921 tataaaccgg atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg
    10981 cgctaatgaa caatcaacgc ctgagatttt cactcgtcag tgtgaacaca attttcccct
    11041 caccctaacc cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc
    11101 atcagtctga ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt
    11161 accgttggac gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg
    11221 tttgatgccc gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt
    11281 ttcaatgagc ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag
    11341 cggaatgccg cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata
    11401 gccgaaatgc tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg
    11461 gaagacaaac ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg
    11521 cactcccgcc cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag
    11581 atactctttc tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt
    11641 ggtgaggaaa atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt
    11701 gctgtggtta acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat
    11761 accgacgggc ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa
    11821 ctgaccgttt actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt
    11881 gccattaagg aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat
    11941 tccgctttcg ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc
    12001 tgtaaaacgc ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt
    12061 cgaaattaaa tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc
    12121 aaatttgcct ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg
    12181 atgactacta cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag
    12241 agtgaaaaat cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa
    12301 agcgtatttg cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc
    12361 gtttgattgt gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg
    12421 cgcgcgaact gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc
    12481 agggaattgt caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc
    12541 aactgaagca aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg
    12601 aagagactca actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg
    12661 agcctggcga tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact
    12721 tgccgcaagc ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg
    12781 gtaaactcga aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga
    12841 aaggggaatc gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg
    12901 ggatcatgca gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg
    12961 ccctggtggg aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg
    13021 gtggtgaaga gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt
    13081 gaagaaaaat acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta
    13141 acgaatagca gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa
    13201 taaaaattac ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta
    13261 aatttgcagg ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt
    13321 gtagaggaat tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat
    13381 ggtcaatatg aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa
    13441 gaggtaaatc atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc
    13501 aataacgccg gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag
    13561 tatgaactca acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt
    13621 ctgatgtcgc aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat
    13681 gtttcctcag aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa
    13741 gccgcgctca atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt
    13801 gtggtcggta tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa
    13861 gaggcgctgg cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa
    13921 aacgccattc ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat
    13981 ctgctgtctg aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa
    14041 acgcgcggat aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct
    14101 tgcgccatgc tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa
    14161 tttgtaccag gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt
    14221 cacaatcaag atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg
    14281 gcagcgatgc ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg
    14341 ctggcgctcg aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac
    14401 ctgacgcaaa tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa
    14461 gatttctgat gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca
    14521 ctaccgtctg gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt
    14581 ataacgatga agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta
    14641 acgtggtcaa tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa
    14701 ccgtttttta tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa
    14761 aaataggcac cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca
    14821 aagcggtttc attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg
    14881 tcataatgga tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa
    14941 acgaacagtt aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg
    15001 gatgacgaca cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat
    15061 atttcttttt tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg
    15121 cccgttaatt gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat
    15181 actcggtggt acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc
    15241 tccggattct gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag
    15301 catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt
    15361 gtttgcccgt accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc
    15421 gcggtttcgc actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt
    15481 tgctattccg gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt
    15541 aaacgccatt cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt
    15601 ggtcggttac gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt
    15661 cctcggtttc ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg
    15721 cgtgatcatg gccctggtct acatccagct aaatccacag tggcgtaaag ctgaacctca
    15781 actccaggcc accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg
    15841 aacagagaaa aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac
    15901 aggcgtcctt taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg
    15961 ccatcaagcg actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg
    16021 tgttcttcaa taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg
    16081 aagaggcgcg ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg
    16141 gtctgatggg accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga
    16201 ttaccgctgc gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt
    16261 ttttctttat tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct
    16321 ttcgcaaagg ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg
    16381 aaggcgcgtc gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa
    16441 tcaacgtacc gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga
    16501 ccgtgcagaa cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac
    16561 tgatggttcg tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg
    16621 gcttagggat tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac
    16681 tgccggggcc atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg
    16741 gtaaacgtga tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc
    16801 tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga
    16861 aaaccataaa aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt
    16921 ttgcggcgat attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg
    16981 ttatgtgatt caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt
    17041 cccgtgggtg ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga
    17101 ttgcctgctg gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc
    17161 ctgcgtgatt ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac
    17221 gatggggatt cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt
    17281 gttggcgatt gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga
    17341 taccgacaac gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact
    17401 gattcattat ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg
    17461 tcacggcttc gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc
    17521 ttccttgctg gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt
    17581 cagcgcgcca attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc
    17641 aggcggcaat accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca
    17701 ggccgcaaaa gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga
    17761 attgcctgcc gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac
    17821 gtcactcacg cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat
    17881 ctggtccggt gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta
    17941 atcgcgctac ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat
    18001 gtgcggctga ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc
    18061 ttaaggggcg ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag
    18121 ccttattcgg cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg
    18181 taagcgtagc gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac
    18241 gtgacatcgt gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg
    18301 atttcttact aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg
    18361 gtaaagcctg gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct
    18421 cagcggtgtt tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa
    18481 cggctgcggt tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc
    18541 ccgttgccgc gattgaaaat gctgagatcg tgattgtcgg ggggcgggaa tactttccag
    18601 ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt
    18661 ggtgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc
    18721 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgctg
    18781 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag
    18841 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa
    18901 ggtaactgga tcacggtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat
    18961 gtgtttaagg ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt ttaagtgaat
    19021 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt
    19081 atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg
    19141 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca tatctcccag
    19201 taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc
    19261 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc tgcggcgcaa
    19321 acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact
    19381 gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg
    19441 tgctgccagc gatttatcag caatttcgct gcccatgcgc tcgacgatat cggaagcctg
    19501 ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt cttctgccag
    19561 ttcctctttt ggcattcgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc
    19621 atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag
    19681 cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc
    19741 cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
    19801 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa
    19861 tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc
    19921 cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa acggcaggat
    19981 aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc gggctgcggc
    20041 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag caccaatcac
    20101 cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg cggtcagcag
    20161 tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag
    20221 cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg
    20281 cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac
    20341 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
    20401 aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt
    20461 cccgacatct gcacccagca caatcaccag agccggtgcg agggctacca gatcctgagc
    20521 gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc
    20581 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa
    20641 acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag
    20701 ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc cttatcgtta
    20761 tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac
    20821 tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt
    20881 gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag
    20941 tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac
    21001 atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc gctgaatttg
    21061 cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa ccgatgcacc
    21121 aggccacatg gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag
    21181 ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg gtgccggacg
    21241 gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata
    21301 gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg
    21361 gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc
    21421 agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt
    21481 accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc
    21541 ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta
    21601 gatttcaatg tcatcgccca cacggtttgc cgggaacaag cccaccacgc cacgcggatt
    21661 cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg
    21721 cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg ccagcgacca
    21781 ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc tggcttcaac
    21841 ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaaagcgaa
    21901 gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct tgcgcccgtg
    21961 ctgaatacgt acggtttcat actccttgcg ggtacgagcg acaaaatcat cgcgctgagt
    22021 atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca catacaccgt
    22081 cgggccgctg tagttctgct caattttcac cgccgtgtgc gcttttgagg tcgtcgcgcc
    22141 accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca cgttaaccat
    22201 ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat tcacttcttt
    22261 agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt
    22321 gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt cgcccttcac
    22381 ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt caataaacgg
    22441 ttcgaggtag gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa
    22501 cattttcccc tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc
    22561 aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct gctcgataaa
    22621 ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc acgagcgcca
    22681 ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt tctcggcaag
    22741 ctccagtaaa cgttcagtgc catcatcgcg acgattaaga atcacatctt ccaccgcgtc
    22801 gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat
    22861 atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt cgcgcaccgg
    22921 ttcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg
    22981 cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt tgttgtgctc
    23041 ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa
    23101 gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg
    23161 ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc accgctgcac cgtaacgacg
    23221 caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga tagagttaac
    23281 aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt ttgaggagtc
    23341 gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac
    23401 catcgccgct tcggcgtcga gcatcccttc atccatattg atatcgataa tctgcgcgcc
    23461 gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt ctttgatcag
    23521 gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca caaacaggct
    23581 atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg ggatttccgg
    23641 cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgtggcgt
    23701 ggtgccacag cagccgccga cgatattgag aaagcccgct tccgcccatt cacgtatttg
    23761 ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta gcccggcgtt
    23821 cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt actggcgcag
    23881 ttcatcgggc cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga
    23941 gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt
    24001 gccagagatc ataatcggca gctcaacgcc cagcgtttca aactccgttt ttaccgcaaa
    24061 taccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc
    24121 accttccacc agcgctttgg tggactctcg ataagccacc accagcccgt caaaagtgat
    24181 attacgaaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc
    24241 gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca
    24301 ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta
    24361 atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc
    24421 ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc
    24481 gttgttgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc
    24541 gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca gaatacgttc
    24601 atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgctcaa
    24661 cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt ggtaaaagcg
    24721 accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt
    24781 gcattaacta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg agactgtcat
    24841 ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac
    24901 tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa
    24961 tggcagtgtg gcactcacgg aactggcgca acaagccggg ttacccaatt ccacgaccca
    25021 ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg aactgggaca
    25081 ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt
    25141 gttagcgatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa
    25201 tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgatcagg tacagtgtac
    25261 tcacctgatg cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg cttccggtgc
    25321 aggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa
    25381 agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa aagaagatct
    25441 cgcccaaacg cgcaaacggg gctattcatt tgacgatgag gaacatgcac tggggctacg
    25501 ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa tttctatttc
    25561 cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga tggtgattaa
    25621 agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag
    25681 aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca
    25741 ttgacgccaa aataactt
//
LOCUS       DAZOZD010000037        25724 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOZD010000037 DAZOZD010000000
VERSION     DAZOZD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.037"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..485
                     /gene="gspA"
                     /locus_tag="ACHUPB_001214"
     CDS             <1..485
                     /gene="gspA"
                     /locus_tag="ACHUPB_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0758086.1"
                     /translation="SLQTLVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTW
                     TLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAE
                     WRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK
                     "
     gene            487..906
                     /gene="gspB"
                     /locus_tag="ACHUPB_001215"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="ACHUPB_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFR0758087.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUPB_001216"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHUPB_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0758088.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUPB_001217"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="ACHUPB_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0758089.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUPB_001218"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="ACHUPB_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0758090.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUPB_001219"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="ACHUPB_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0758091.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUPB_001220"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="ACHUPB_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0758092.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUPB_001221"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHUPB_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0758093.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUPB_001222"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="ACHUPB_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0758094.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUPB_001223"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHUPB_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0758095.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUPB_001224"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="ACHUPB_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0758096.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUPB_001225"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHUPB_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0758097.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUPB_001226"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHUPB_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0758098.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUPB_001227"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="ACHUPB_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0758099.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUPB_001228"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="ACHUPB_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0758100.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUPB_001229"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="ACHUPB_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0758101.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUPB_001230"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHUPB_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0758102.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUPB_001231"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHUPB_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0758103.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUPB_001232"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="ACHUPB_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0758104.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUPB_001233"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="ACHUPB_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0758105.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUPB_001234"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHUPB_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0758106.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUPB_001235"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHUPB_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0758107.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUPB_001236"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="ACHUPB_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0758108.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="ACHUPB_001237"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="ACHUPB_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0758109.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUPB_001238"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHUPB_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0758110.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUPB_001239"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHUPB_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0758111.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUPB_001240"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHUPB_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0758112.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUPB_001241"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHUPB_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0758113.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUPB_001242"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHUPB_001242"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0758114.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUPB_001243"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHUPB_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0758115.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="ACHUPB_001244"
     CDS             14905..15273
                     /locus_tag="ACHUPB_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR0758116.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUPB_001245"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="ACHUPB_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR0758117.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUPB_001246"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="ACHUPB_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR0758118.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUPB_001247"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHUPB_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR0758119.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUPB_001248"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHUPB_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0758120.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUPB_001249"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHUPB_001249"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0758121.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUPB_001250"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHUPB_001250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0758122.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUPB_001251"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHUPB_001251"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0758123.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUPB_001252"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="ACHUPB_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0758124.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /gene="smg"
                     /locus_tag="ACHUPB_001253"
     CDS             21950..22423
                     /gene="smg"
                     /locus_tag="ACHUPB_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0758125.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="ACHUPB_001254"
     CDS             22452..22994
                     /locus_tag="ACHUPB_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0758126.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUPB_001255"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHUPB_001255"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0758127.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUPB_001256"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="ACHUPB_001256"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0758128.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="ACHUPB_001257"
     CDS             24391..24648
                     /locus_tag="ACHUPB_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0758129.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="ACHUPB_001258"
     CDS             complement(24624..25178)
                     /locus_tag="ACHUPB_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0758130.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25651..>25724
                     /locus_tag="ACHUPB_001259"
     rRNA            25651..>25724
                     /locus_tag="ACHUPB_001259"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6567 a         6071 c         6727 g         6359 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    25501 cgcaactgct ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc
    25561 ttaacgtcgc aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac
    25621 gaagtttaat tctttgagca tcaaactttt aaattgaaga gtttgatcat ggctcagatt
    25681 gaacgctggc ggcaggccta acacatgcaa gtcgaacggt aaca
//
LOCUS       DAZOZD010000038        25647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOZD010000038 DAZOZD010000000
VERSION     DAZOZD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.038"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUPB_001260"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHUPB_001260"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0758131.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUPB_001261"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHUPB_001261"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0758132.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUPB_001262"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHUPB_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0758133.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUPB_001263"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHUPB_001263"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0758134.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="ACHUPB_001264"
     CDS             complement(5499..5696)
                     /locus_tag="ACHUPB_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0758135.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUPB_001265"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHUPB_001265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0758136.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUPB_001266"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHUPB_001266"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0758137.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUPB_001267"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHUPB_001267"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0758138.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUPB_001268"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHUPB_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0758139.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUPB_001269"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHUPB_001269"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0758140.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUPB_001270"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHUPB_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0758141.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUPB_001271"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHUPB_001271"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0758142.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUPB_001272"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHUPB_001272"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0758143.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUPB_001273"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHUPB_001273"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0758144.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="ACHUPB_001274"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="ACHUPB_001274"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0758145.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="ACHUPB_001275"
     CDS             complement(17138..17191)
                     /locus_tag="ACHUPB_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758146.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="ACHUPB_001276"
     tRNA            complement(17425..17500)
                     /locus_tag="ACHUPB_001276"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="ACHUPB_001277"
     tRNA            complement(17536..17611)
                     /locus_tag="ACHUPB_001277"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="ACHUPB_001278"
     tRNA            complement(17769..17844)
                     /locus_tag="ACHUPB_001278"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="ACHUPB_001279"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="ACHUPB_001279"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0758147.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="ACHUPB_001280"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="ACHUPB_001280"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0758148.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="asd"
                     /locus_tag="ACHUPB_001281"
     CDS             19844..20812
                     /gene="asd"
                     /locus_tag="ACHUPB_001281"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0758149.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="ACHUPB_001282"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="ACHUPB_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0758150.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="ACHUPB_001283"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="ACHUPB_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0758151.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7194 c         6499 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAZOZD010000039        25104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOZD010000039 DAZOZD010000000
VERSION     DAZOZD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.039"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            194..382
                     /gene="bcsR"
                     /locus_tag="ACHUPB_001284"
     CDS             194..382
                     /gene="bcsR"
                     /locus_tag="ACHUPB_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0758152.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHUPB_001285"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHUPB_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0758153.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="ACHUPB_001286"
     CDS             1143..1307
                     /locus_tag="ACHUPB_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR0758154.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHUPB_001287"
                     /pseudo
     CDS             1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHUPB_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHUPB_001288"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHUPB_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0758155.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHUPB_001289"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHUPB_001289"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0758156.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHUPB_001290"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHUPB_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHUPB_001291"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHUPB_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0758157.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="ACHUPB_001292"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="ACHUPB_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0758158.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="ACHUPB_001293"
     CDS             14457..15953
                     /locus_tag="ACHUPB_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0758159.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHUPB_001294"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHUPB_001294"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0758160.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHUPB_001295"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHUPB_001295"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0758161.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="ACHUPB_001296"
     CDS             18047..20107
                     /locus_tag="ACHUPB_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0758162.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKFSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="ACHUPB_001297"
     CDS             complement(20289..21611)
                     /locus_tag="ACHUPB_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0758163.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHUPB_001298"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHUPB_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0758164.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="ACHUPB_001299"
     CDS             complement(23084..23983)
                     /locus_tag="ACHUPB_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0758165.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="ACHUPB_001300"
     CDS             24503..>25104
                     /locus_tag="ACHUPB_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0758166.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6990 g         5855 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaat tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAZOZD010000040        24578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOZD010000040 DAZOZD010000000
VERSION     DAZOZD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.040"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            285..2306
                     /gene="uvrB"
                     /locus_tag="ACHUPB_001301"
     CDS             285..2306
                     /gene="uvrB"
                     /locus_tag="ACHUPB_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0758167.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2498..3406)
                     /gene="yvcK"
                     /locus_tag="ACHUPB_001302"
     CDS             complement(2498..3406)
                     /gene="yvcK"
                     /locus_tag="ACHUPB_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0758168.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3671..3815
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3803..4792
                     /gene="moaA"
                     /locus_tag="ACHUPB_001303"
     CDS             3803..4792
                     /gene="moaA"
                     /locus_tag="ACHUPB_001303"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0758169.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4814..5326
                     /gene="moaB"
                     /locus_tag="ACHUPB_001304"
     CDS             4814..5326
                     /gene="moaB"
                     /locus_tag="ACHUPB_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0758170.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5329..5814
                     /gene="moaC"
                     /locus_tag="ACHUPB_001305"
     CDS             5329..5814
                     /gene="moaC"
                     /locus_tag="ACHUPB_001305"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0758171.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5807..6052
                     /gene="moaD"
                     /locus_tag="ACHUPB_001306"
     CDS             5807..6052
                     /gene="moaD"
                     /locus_tag="ACHUPB_001306"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0758172.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6054..6506
                     /gene="moaE"
                     /locus_tag="ACHUPB_001307"
     CDS             6054..6506
                     /gene="moaE"
                     /locus_tag="ACHUPB_001307"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0758173.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            6643..7347
                     /locus_tag="ACHUPB_001308"
     CDS             6643..7347
                     /locus_tag="ACHUPB_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0758174.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7618..8265
                     /locus_tag="ACHUPB_001309"
     CDS             7618..8265
                     /locus_tag="ACHUPB_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0758175.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(8330..9041)
                     /locus_tag="ACHUPB_001310"
                     /pseudo
     CDS             complement(8330..9041)
                     /locus_tag="ACHUPB_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(9041..10282)
                     /gene="clsB"
                     /locus_tag="ACHUPB_001311"
     CDS             complement(9041..10282)
                     /gene="clsB"
                     /locus_tag="ACHUPB_001311"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0758176.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(10279..11040)
                     /locus_tag="ACHUPB_001312"
     CDS             complement(10279..11040)
                     /locus_tag="ACHUPB_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0758177.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11173..11583
                     /locus_tag="ACHUPB_001313"
     CDS             11173..11583
                     /locus_tag="ACHUPB_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0758178.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11545..12651)
                     /locus_tag="ACHUPB_001314"
     CDS             complement(11545..12651)
                     /locus_tag="ACHUPB_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0758179.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12662..13795)
                     /locus_tag="ACHUPB_001315"
     CDS             complement(12662..13795)
                     /locus_tag="ACHUPB_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0758180.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(13788..15524)
                     /locus_tag="ACHUPB_001316"
     CDS             complement(13788..15524)
                     /locus_tag="ACHUPB_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0758181.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15517..16512)
                     /gene="hlyD"
                     /locus_tag="ACHUPB_001317"
     CDS             complement(15517..16512)
                     /gene="hlyD"
                     /locus_tag="ACHUPB_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0758182.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(16515..17186)
                     /gene="cecR"
                     /locus_tag="ACHUPB_001318"
     CDS             complement(16515..17186)
                     /gene="cecR"
                     /locus_tag="ACHUPB_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0758183.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17415..18779
                     /gene="rhlE"
                     /locus_tag="ACHUPB_001319"
     CDS             17415..18779
                     /gene="rhlE"
                     /locus_tag="ACHUPB_001319"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0758184.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(19011..19493)
                     /gene="ybiA"
                     /locus_tag="ACHUPB_001320"
     CDS             complement(19011..19493)
                     /gene="ybiA"
                     /locus_tag="ACHUPB_001320"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0758185.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            19613..21763
                     /gene="dinG"
                     /locus_tag="ACHUPB_001321"
     CDS             19613..21763
                     /gene="dinG"
                     /locus_tag="ACHUPB_001321"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0758186.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            21791..22753
                     /gene="ybiB"
                     /locus_tag="ACHUPB_001322"
     CDS             21791..22753
                     /gene="ybiB"
                     /locus_tag="ACHUPB_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0758187.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            22894..23979
                     /gene="hcxB"
                     /locus_tag="ACHUPB_001323"
     CDS             22894..23979
                     /gene="hcxB"
                     /locus_tag="ACHUPB_001323"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0758188.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            24046..24105
                     /gene="ybiE"
                     /locus_tag="ACHUPB_001324"
     CDS             24046..24105
                     /gene="ybiE"
                     /locus_tag="ACHUPB_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0758189.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(24208..24468)
                     /gene="ybiJ"
                     /locus_tag="ACHUPB_001325"
     CDS             complement(24208..24468)
                     /gene="ybiJ"
                     /locus_tag="ACHUPB_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0758190.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         5808 a         6374 c         6620 g         5776 t
ORIGIN      
        1 ataaatgcaa tggcagtcac tgaacaggca tctcttgcca taaaactgtc atcactcatc
       61 ttgacaaatg ttaaaaaagc cgttgctttg gggataaccc ggtaaggccg gagttttatc
      121 tcgccacaga gtaaattttg ctcatgattg acagcggagt ttacgctgta tcagaaatat
      181 tatggtgatg aactgttttt ttatccagta taatttgttg gcataattaa gtacgacgag
      241 taaaattaca tacctgcccg cccaactcct tcaggtagcg actcatgagt aaaccgttca
      301 aactgaattc cgcttttaaa ccttctggcg atcagccaga ggcgattcga cgtctcgaag
      361 aggggctgga agatggcctg gcgcaccaga cgttacttgg cgtgactggc tcagggaaaa
      421 ccttcaccat tgccaatgtc attgctgacc ttcagcgccc aaccatggta cttgcgccca
      481 acaaaacgct ggcggcccag ctgtatggcg aaatgaaaga gttcttcccg gaaaacgcgg
      541 tggagtattt cgtctcctac tacgactact atcagccgga agcctatgta ccgagttccg
      601 acactttcat tgagaaagat gcctcggtta acgaacatat tgagcagatg cgtttatccg
      661 ccaccaaagc gatgctggag cggcgtgatg tggttgtagt ggcctctgtt tccgcgattt
      721 atggtctggg cgatcctgat ttatatctca agatgatgct ccatctcacg gtcggtatga
      781 ttatcgatca gcgcgcgatt ctgcgccgac tggcggagct gcaatacgct cgtaatgatc
      841 aagcattcca gcgtggtact ttccgcgttc gtggcgaggt gatagatatc ttcccggcag
      901 aatcggatga cattgcactt cgcgtggaac tgtttgacga ggaagtggaa cgattgtcgt
      961 tatttgaccc gctgaccggg cagattgttt ccactattcc acgttttacc atctacccga
     1021 aaacgcacta cgtcacaccg cgcgagcgca tcgtacaggc gatggaggag atcaaagaag
     1081 aactggccgc cagacgcaaa gtgctgttgg aaaacaacaa actgctggaa gagcagcggc
     1141 tgacccagcg tacccagttt gatctggaga tgatgaacga gctgggctac tgttcgggga
     1201 ttgaaaacta ctcgcgcttc ctctccggtc gtggaccggg tgagccaccg ccgacgctgt
     1261 ttgattacct gcctgccgat gggctgctgg tggtcgatga atctcacgtc accattccac
     1321 aaattggcgg catgtatcgc ggtgaccggg cgcgtaaaga gacactggtg gagtacggct
     1381 tccgcctgcc atcagcgctg gataaccgtc cgcttaagtt tgaagagttc gaagcattag
     1441 cgccgcaaac catctatgtt tcggcgacgc cgggtaatta cgagctggaa aaatccggcg
     1501 gcgatgtggt ggatcaggtg gtgcgtccaa ccggattgct tgacccgatt atcgaagtgc
     1561 ggccggtggc gacacaggtt gatgatcttc tttcggagat tcgtcagcga gcggcaatta
     1621 acgaacgcgt actggtcacc acactgacca agcggatggc ggaagatctt accgaatatc
     1681 tcgaagaaca tggcgagcgc gtgcgttatc ttcattcaga tatcgacacc gtcgaacgta
     1741 tggagattat ccgcgacttg cgtctgggtg agttcgacgt gctggtaggg atcaacttac
     1801 tgcgcgaagg tctggatatg ccggaagtgt cgctggtggc gatcctcgac gctgacaaag
     1861 aaggcttcct gcgttccgaa cgttcgttga tccagactat tggtcgtgcg gcacgtaacg
     1921 ttaacggtaa agcgattctc tacggcgata agatcacccc atcaatggcg aaagcgattg
     1981 gcgaaaccga acgtcgccgt gagaaacagc agaagtacaa cgaggaacac ggaattacgc
     2041 cgcaaggctt gaacaagaaa gtggtcgata tcctggcgct ggggcagaac attgccaaaa
     2101 ccaaagcgaa gggcagagga aaatcgcgcc cgattgttga gccggataat gtgccgatgg
     2161 atatgtcgcc taaagcgttg caacagaaaa tccatgagct ggaagggtta atgatgcaac
     2221 acgcgcagaa tctggagttc gaagaagcgg cacaaattcg tgaccagttg catcagctgc
     2281 gtgagctgtt tatcgcggct tcgtgacagg ataacgaaga agacagatga caaaagcaaa
     2341 attgcctgat gtgctacgct tatcaggcct acatgatctc tgcaatacat tgaattagcg
     2401 cggtttgtag gccggataag gcgttcacgc cgcatccggc ataaacaacg cgcactttgt
     2461 cttcaatagg aagccggaat ttttcttcgg atttccgtta acctaaagcc tgtaacgcct
     2521 tttccagcgc gttatgtaac aactggcggt catgacgata cggaatatcg ctggcctcca
     2581 gtacctcctg aatcacaatc cgctctttca ccgccgagac atccactttt ggcccgacga
     2641 tgaccgcatc aatgaccttt ttaccaacat actgctccat aattgccagc ttgctttcca
     2701 gcttcaaatt agccgcaggt aaactcaact cacgccccag attgccgata tagaccatcg
     2761 gcgctggcgt gcggcgtaat gcctgggcga tttccttcag cagcagaatt ggcatcaggc
     2821 tggtataaaa actgccaggc ccaataatga tgagatccgc ttcattgata gcgtgaaccg
     2881 cctcacgcgt tgcgggtaca ttcggcgtta ataataactc ttgaatcggc gtagttaact
     2941 ggtcgatatt gacctcgccg taaacttcat gcccctgatc gtcaatcgcc atcagatcga
     3001 cgggatgctc tgacattgga atcaaatgcg tatccacttt cagcagatta cgaattaaat
     3061 tgatggcttc cagaggccgc acgctaaggt gatccagcgc ctttaacatc aagtttccga
     3121 gattatgacc ggaaagttcg ccattgccgc caaaacggta ttcaaacatc gcggaggcga
     3181 cgctcggttc cgttatcagt tggttgaggc agttgcgcat atcgccccag gcaatgccgc
     3241 cttctgaacg gcgaatacgc cccgtcgagc caccattatc cgtggtggtg acgatacccg
     3301 ttaaacgaga acccaaagac gaaagtgatg agagaacgcg tcccagtcca tgccctccgc
     3361 cgagagcaac gacacgatca agatcagcca gcgtacgatt gcgcatatag tttcctggag
     3421 tcagattatc cgcgctacag tagcgcaaat tgcggggaaa cagcaattaa cctgccaata
     3481 ttgaggatac aaactatttt cttctacctc taaaggacga tgcacgctat gcctccctga
     3541 tgatgtatat caaagtaaaa ctgccatttt cccttattct gtagcgaaat agcacgatca
     3601 tgacgctata tacatgatta catagcgaaa gtgtggatgg taaaaatctc atttacacgc
     3661 tagtatcggc ttaaccacta aacactctag cctctgcacc tgggtcaact gatacggtgc
     3721 tttggccgtg acaatgctcg taaagattgc caccagggcg aaggaagaaa tgacttcgcc
     3781 tcccgtatct ggaaaggtgt acatggcttc acaactgact gatgcatttg cgcgtaagtt
     3841 ttactacttg cgcctgtcga ttaccgatgt gtgtaacttt cgttgcacct actgcctgcc
     3901 ggatggctac aaaccgagcg gcgtcaccaa taaaggcttt cttaccgtcg atgaaattcg
     3961 ccgggttacg cgcgccttcg ccagtctggg caccgaaaaa gtgcgcctga caggaggaga
     4021 gccgtcttta cgccgcgact ttaccgatat catcgccgct gtgcgggaaa acgacgctat
     4081 ccgccagatt gcggtcacca ccaatggtta ccgtctggaa cgcgatgtgg cgaactggcg
     4141 cgatgcggga cttactggca ttaacgtcag tgtcgacagt ctggacgccc gccagtttca
     4201 cgctattacc gggcaggata aattcaacca ggtcatggca gggatcgatg ctgcatttga
     4261 ggtcggtttt gagaaggtca aagtcaatac cgtgctgatg cgtgatgtta atcatcacca
     4321 gctcgacacc tttctgaact ggatccagca tcgccctatc cagctgcgtt tcatcgaact
     4381 gatggaaacg ggcgagggca gtgagctctt ccgtaagcat cacatctctg gtcaggttct
     4441 gcgtgacgag ctactgcgtc gcggctggat ccaccaatta cgtcaacgca gcgacggtcc
     4501 tgcgcaagtc ttttgccatc cagattacgc cggagagatt ggccttatca tgccgtatga
     4561 aaaagacttc tgcgccactt gcaaccgcct gcgcgtttcc tccattggta aactccatct
     4621 ctgcctgttt ggtgaaggcg gcgttaacct gcgcgatctg ctggaagacg atacccagca
     4681 acaggcgctg gaagcgcgta tttcagcggc gctgcgggag aagaaacaga cccatttcct
     4741 gcatcaaaac aacaccggta ttacgcaaaa cttatcgtac attggcggct aaaacgtcaa
     4801 aaggagagat cagatgagtc aggtaagcac tgaatttatc ccgacccgta ttgctattct
     4861 tacggtttct aatcgtcgcg gtgaagaaga cgatacctcc ggtcactatt tgcgcgattc
     4921 ggcgcaagaa gcgggccatc acgttgtcga taaagccatt gtgaaagaaa accgctacgc
     4981 tattcgcgct caggtatctg cgtggatcgc cagcgacgat gtacaagtgg tgttgattac
     5041 gggggggact ggcctgacgg aaggtgatca ggctcccgaa gcattgctgc cgttgttcga
     5101 ccgtgaagtt gaaggttttg gtgaagtgtt ccggatgttg tcgtttgaag agattggcac
     5161 ttccacgttg caatctcgtg cggtagcggg cgtcgccaac aaaacgctga ttttcgccat
     5221 gccgggttcg accaaagcgt gccgtaccgc atgggaaaat atcatcgcgc cgcagctgga
     5281 tgcccgtacg cgtccgtgta atttccatcc acatttgaag aaataagtat gtcgcaactg
     5341 acccatatca acgccgctgg cgaagcgcac atggtggatg tctccgccaa agcggaaacc
     5401 gtgcgtgaag cgcgggcgga agcctttgtc accatgcgca gcgagacgct ggcgatgatt
     5461 attgatggtc gccaccacaa aggcgacgta tttgccactg cgcgtattgt cggtattcag
     5521 gcggcaaaac gcacctggga tctgatcccg ctgtgtcacc cgctgatgct cagcaaagtt
     5581 gaagtcaatt tacaggccga gccggagcac aatcgggtgc gtatagaaac cttatgccgc
     5641 ctgaccggga aaaccggtgt cgaaatggaa gcgttaaccg cggcctccgt ggcggcgctg
     5701 accatttatg acatgtgcaa agcggtgcaa aaagatatgg tgattggtcc ggtacgtttg
     5761 ctggcgaaga gcggcggcaa gtcgggtgac tttaaggtgg aagcggatga ttaaagttct
     5821 ttttttcgcc caggtgcgcg agttggtggg aacagatgca accgaagtgg ctgcggatta
     5881 cccaaccgtt gaagcgttac gccagcacct ggctgcgcag agcgatcgct gggcgctggc
     5941 gctggaagat ggcaaattgc tggctgccgt caaccagacg ctggtgagtt ttgaccatcc
     6001 gctgactgac ggcgacgaag tagctttctt cccgccggta accggaggtt aagatggcag
     6061 aaaccaaaat tgttgtcggc ccgcagccgt tcagcgtagg agaagagtac ccgtggctgg
     6121 ctgagcgtga cgaagacggt gcagtggtca cgtttaccgg taaggtgcgc aaccataacc
     6181 tgggtgacag cgtcaaagca ttaaccctcg aacactatcc ggggatgact gaaaaagcac
     6241 tggcagaaat cgttgatgaa gcgcgtaacc gctggccgct ggggcgcgtc acggtgattc
     6301 accgcatcgg ggaattatgg ccgggcgatg aaatcgtttt tgtcggtgtc accagtgcgc
     6361 atcgcagcag tgcgtttgaa gccgggcagt ttattatgga ttatctcaaa acccgcgcac
     6421 cgttctggaa gcgcgaagcc acgccggaag gcgaccgctg ggttgaagcc cgggagagcg
     6481 atcagcaggc ggcaaaacgc tggtagtttt ttgttagccg gataaggcac cgctgcgtcc
     6541 ggcaatttca ccgctcagta catgcgttta cgctgcgtcg atgcacagcc tcatcatttt
     6601 gcagtatcct taagatattc cttatatctt caggagatcg tcatggacag attcccacgt
     6661 tctgattcaa tcgtacaacc ccgggctggc ttgcaaactt atatggcgca agtctatggc
     6721 tggatgaccg ttggcttgtt gctgaccgca tttgttgcct ggtatgcggc taattccgcg
     6781 gccgtgatgg agctgttgtt cactaaccgt gtctttttaa tcggtctgat catcgcgcaa
     6841 ttagcattgg ttattgtgtt atcagcgatg attcaaaagc tgagcgcagg tgtaacgacg
     6901 atgctcttta tgctttattc ggcgctgacg ggtcttacgc tttccagtat attcattgtc
     6961 tataccgctg cttctatcgc cagtactttc gtcgttactg ccgggatgtt cggcgcaatg
     7021 agcctgtacg gttacaccac gaagcgcgat ttaagtggct ttggcaatat gctgtttatg
     7081 gcgttaatcg gcattgtgct ggcatcgctg gtcaacttct ggctgaaaag cgaagcattg
     7141 atgtgggcag ttacctacat cggcgtgatt gtctttgtcg gattgacggc gtatgacact
     7201 cagaaattga aaaatatggg tgagcagatt gatacccgcg acacgtcgaa cctgcgcaaa
     7261 tattccattc ttggcgcgtt aaccttgtat ctggacttca tcaacctgtt cctgatgttg
     7321 ttacggatct tcggcaaccg ccgttaattc tttttcacca tcactatccc ggccatgtgt
     7381 cgggattttt ttcttactaa aaacgacact taacaagtac caggtataaa atatgcctta
     7441 tgagcgagcg gaatcgtttt ccgtatccat aaatacaaaa gtattttgta tgcgtggttt
     7501 taaatttatt gatggtgaat attaatattt tttctaagga caactaaaag aatggaatca
     7561 tactcgcaaa acagcaataa attagatttt cagcacgagg ccaggatata aaacggtata
     7621 tggctcatta ccgctttagg tttggtggca accgcaggac tagcctgggg agctaagtat
     7681 ctcgaaatta cggcaaccaa atatgattca ccaccaatgt atgtcgctat agggttatta
     7741 ttactttgta tgtatggctt aagtaaggat atcaacaaga taaatgccgc catcgcgggc
     7801 gtaatatatc tgtttttact ctctttggtg gcgattgtcg ttgcaagttt agttcctgta
     7861 tatgccatta tcatcgtgtt cagcactgcg ggcgcgatgt ttttaatcag tatgctggcc
     7921 ggtttattat ttaatgttga gcctggttct caccgtttta tcattatgat gacgttgaca
     7981 gggttggccc tggtaatcat cgtgaatgcg gcattaatga gtgaacggcc catttgggta
     8041 ataagttgct taatgattgt gttatggcca ggcattatct cgcatggacg aaataagctc
     8101 cttgaactgg caggaaaatg ccatagtgaa gagttgtgga gtccggttcg ttgcgctttt
     8161 acaggtgcat taacactcta ttactatttt atcggcttct ttgggatact tgccgcgata
     8221 gctataacgc ttgtctggca aaggcatacg cgtttttttc attagccgta aatgcacata
     8281 ctaaagaact taactaaact tcacatcgcc gcttcatttt tcgcccgtag cccagtacaa
     8341 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
     8401 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
     8461 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
     8521 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtgat
     8581 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
     8641 agttggtggt aatactgagc gagaaaatcc gcgtaatagt gctaccgggc aaccccagac
     8701 gagagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
     8761 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
     8821 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcgacc
     8881 gcactaagca gcgcaacgcg attgtagtcg cggatgacct tccagacctc ttcccagtcc
     8941 acttttttgg cgtagaccac cagcaacaca atcaccgcga taaaaaacag ccaggtgagg
     9001 atcttctttg ctaagcgcca gcgcgggtgt gatttactca tcagggtttt acccccgtgt
     9061 tttcagtttc tacccgatcc tgcgtttcca ttgtcggttg tgcgggcgga tcaacctgcg
     9121 ccagacgtgg cgtgtgtgcc ggaagccagc caaccagcgc cgggaagtgg cgtaaaaagt
     9181 ggaacgccag cacgcttttg gtcaggttcc accaggtgcg tttgggcagc atggtttcat
     9241 ccacctgctg acaatctgcg gcgataatgc cgttcagatt atcgcgcagc gtctggttaa
     9301 aattacgatc gtggatgatg acatttgctt cgagattcag tgacaaactg agcggatcga
     9361 gattactgga ccctactgtc gcccagtgat cgtccatcaa tgctactttg ccgtggagcg
     9421 ggcggcggcg gtactcaaac acctgaacgc cgcctttaac cagatagtta tacagcaagc
     9481 gcgcgccgac tctgacaatc ggcatatccg gttcgccctg aatgatcagt ttgatccgca
     9541 ccccgcggcg tgccgcttta cgcaaggcgt gtaaaaatcg atagccgggg aagaagtagg
     9601 cgttggcgat aatgacttcc cgccgcgcct gagtgagcat tttcagatag tggcgttcta
     9661 tatcatcgcg gtgttcttcg ttatcgcgcc agaccagcaa tacctgcgcg tctcccggct
     9721 ggcggttctc ttccgctttg tgatgacgtc gccaccagcg tcgtgccgcg ctctgtccag
     9781 gcaggttttc cagctcaaac tggagaatat cttcgacaat cggcccttca aggcgtaccg
     9841 cgtaatcctg tttagcctct ggaccgtagc tggacatatg ctcggcggag taattcagcc
     9901 cgccaataaa ggcgatacgc gcgtcgatca ccacaatttt gcgatgcatc cggcgaaaca
     9961 cattggtgcg cataccaaaa aggcgagggc ggggatcgta gtagcggaac actacgccag
    10021 ctgccgtcag ttcattgaca aactcatcgc tgagatccgg cgaaccgtag ccatccagca
    10081 agacttccgc tttaaccccg cgttgcgctg ctgccagtag tgccgcatgc agctgtttgc
    10141 cgacgttatc ctcaaaccag ataaacgttt caagaatgat gcgttcttgt gcctcgccaa
    10201 tcgccttaaa caccgcggga taatattgct cgccgttttc cagcaactgg atcttattgc
    10261 cttcgcgcca gctacatttc ataaatgaat ctccgcactt aaaggggcat gatcagaaag
    10321 gtgtcgccat gtccgcagcg gcaacgcggt tggcgcgctg gcgctggcat ttttgacgta
    10381 gatcctgtcc agtcgtagta gaggaaattg caccggaaac gtgcgcgccg ggcgtccgtg
    10441 ggcgcgggta aaaatctcat ccagtccggc ctgcactttt aacggatgat tagctttttg
    10501 ccgccagtca ttgaaatcac ccgccaccaa taccggttcg ccgtccggta gctcattcac
    10561 ccattcggcg agcatcgcaa gctgcgcctg acggtgcgcc tcacgcaggc ccagatgtac
    10621 gcacatcaca tgaatcgctt ttccggtcat cggcggcaca atgcggcagt agagcacgcc
    10681 gcgcttttcc gcaccatcga ccgaaacatc gcgattctca taatgttcaa tgggataacg
    10741 cgacagtacg gcgttgccgt gatgcccttc cgggtatacg gcattgcgac cgtaggcaaa
    10801 atcgctccac atagtgtcgg cgagaaactc gtagtgcgag gtatcgggcc agttttccac
    10861 atgcagcgga tgaacttcgt gcgcgcccat cacttcctgc aggcaaacaa tatcggcgct
    10921 gacggtacgc acggcatcgc gaagttccgg caaaatgaag cgtcggttaa acgcggtaaa
    10981 gcctttgtga atgttgatgg tgagcacctt gaacgaaaat tgttgtgttt gatcgggcat
    11041 aattttcctg tctttgcctc ttatctcatt gaaatagtgt agtcggcgtc acaaaaaggt
    11101 gcggtcttac ggaattttcc gtaaagttcg gtactctgag taagtagaga taaattcttc
    11161 aggagagaag ccatgaagtg gcaacaacgt gttcgtgtcg caacgggtct aagttgctgg
    11221 cagattatgt tgcatttact ggtagtggcg ctgctggtgg tgggctggat gagtaagact
    11281 ctggttcacg tcggcgtggg attatgcgca ctgtattgtg tcacggtagt gatgatgctg
    11341 gtgtttcagc gccaccccga gcaacgctgg cgtgaggtgg cagacgtgct ggaagagctg
    11401 accacgacct ggtattttgg cgcagcgctg attgtgctgt ggctgttgtc ccgcgtactg
    11461 gaaaacaact ttttgctggc aattgcaggg ctggcgatcc ttgctggccc ggcggtagtg
    11521 tctttgctgg cgaaagataa gaagttacat caccttacgt ctaaacatcg cgtacgccgc
    11581 tgaccctgtc gtggccgtta tcaccagtag cggccacaaa ctattccaca caatatccag
    11641 actcgcatcc ttcaaataaa tctgcttggt aatgtccgta aagtggcgaa tagggttaat
    11701 ccacgtcagg ttttgcagcc atactggcat gttttccacc ggagaaacgt aaccggaaag
    11761 gagaatggcg ggcatcataa aaacaaacac gccgataaac gcctgctgtt gtgtcgagca
    11821 gagtgatgaa atcaacagac cgaatcccac cagcgataaa ccgtaaatca ccatcgtaaa
    11881 gtagaacagc gccagcgatc cggcgaaggg gatttgatac gcccagatac caatcgccag
    11941 cacaatggtg gcctggaagg tggcgacaat taacgccggt acggctttgc cgatgaagat
    12001 ctgccaggtg gtgagcggcg aaaccagtag ctgatcgagc gtaccttgtt cacgttcgcg
    12061 ggcgacggaa agtgaagtga cgatcattac gccgatagtg gtgatcatgg cgatcagcga
    12121 cggcaccaca aaccatttgt agtcaagatt cgggttatac cagttgcgta ccaccagctc
    12181 gctgttgtta ggtttcggtt ttccttccag cagctcctgc tgataatttt tgacgatctg
    12241 ttgcaggtag ttggcggcaa tttgcgcact gttggagtta cgcccgtcga ggatcaactg
    12301 caacggcgcg gtctggaagg tatccagttt gcgcgagaag tcagccggga aacgcaccag
    12361 tagtaacgcc ttttgtgtgt cgatggttgg gcggatctcc tgtgggcttt tcagcagcag
    12421 cacatgggta aaggcgctgg cgcgggcaaa acgttgggtc agctccaccg aatgctcgcc
    12481 gttatcttca tcgtagatgg cgatggtggc gttagtcact tccagcgtgg cggcgaacgg
    12541 gaacaggatc acctgaataa gcacaggtaa aatcagaatc gcgcgggttt gtggttcgcg
    12601 cagcaacgac tgcaactctt tgcggattaa cgtccataag cgatgaaaca tgctcttctc
    12661 cctaatccag ccgacgtttg gttttcagcc acgtcaggcc gataaacatc accgccgaag
    12721 cgatcaaaaa cagcacgttt accaccagca ccactggaat attcccggcg aggaacaggc
    12781 tttgcagggt gctgacgaaa taacgagcgg gaataatgta cgtcaccgcg cggatcaccg
    12841 cgggcatact gtcgatctga aaaataaagc cggaaagcat aatcgacggc aggaacgcgg
    12901 cgttcagggc gacctgagcg gcattgaact ggttgcgggt aatcgtggaa atcagcagcc
    12961 ccatccccag ggtactgagt aaaaacaggc tggagataaa aaacagaata agcagcgaac
    13021 cgcgatacgg cacgccgaga ataaacactg acaccagcat acacagcagc atcgccagca
    13081 tcccgagaaa gtaataaggg atcagcttac acagcagcag ttccgtgcgg gtaatctccg
    13141 tagagagcag agcctccatg gtgccgcgtt cccattctcg cgccaccacc agcgaggtga
    13201 gaatcgcgcc gatgaccgtc atgatgatgg tcactgcacc ggggataatg aagtgctggc
    13261 taatcgccgc cgggttaaac cagtagcggg tttgtacatc aataagcggt tcaaaagtct
    13321 gcccgttgtc ctccgctcgc tgcatttgcc agatctgcca gatcccttcg acatacccct
    13381 gtacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg
    13441 cgttggcgcg ctccatctgt tccgcaaaat ccaccggaat aaccaccaga ccgcgaattt
    13501 tccccgcctg cattttggcg atcagttcct gacggttatc gctgatggtg gcgtcgatgt
    13561 agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc tgttccagca
    13621 agatcccgac ccgcagcttg ctggagtcga ggttaatgcc ataaccaaaa ataaacagca
    13681 gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg atctgccgcg
    13741 tctctttgac gcacagcgcc cgcacgcgac gccaggaaag gatcgggtta ctcattgcta
    13801 tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt gggatcgggt
    13861 tgctcatcgt tagccgactg tgctttcaaa tcgtccggcg taccgctggc gattaatttc
    13921 ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa gtgggtggtg
    13981 accatcaccg tgacgccttt ctctaccatg ctgttgatat gtagccaaaa ttcacggcgg
    14041 gtgaggggat caacgccgga cgtcggttcg tcgagaaaca gaatgtccgg ttcatgcatc
    14101 agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc ggtggcgtgg
    14161 gaggcgatac ttttcaggcc gaacgcctcg ctcatgcggg agattttttc gttctgcgcc
    14221 cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac cgtcaggtta
    14281 ccgtagagcg aaaacttttg cgccatatag ccgagatgct ggcgcgcttt accagaactc
    14341 tctttcagat ccatccccag taccagcgcc tgaccagagg tcggcaccag caaaccgcac
    14401 atcatcttaa aggtggtcga tttgcccgcg ccgtttggcc ccagcaaacc aaaaatctcc
    14461 ccgtgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc
    14521 agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg taatattgcg
    14581 cccagcggcg attccgaggt tccggcaccg cccagcaaat caataaacgc atcttcaaaa
    14641 cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg aatatcgtct
    14701 ggtgtggtct cttttttgag gatcagacgt accgatttcc cctgaatcat gccgtcgctg
    14761 acctgcggca gtttcaatgc ccgttgcaac agtttgcggt tgccctcgtg tggacttgtc
    14821 atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttcccc ctgatacagc
    14881 aactcgcctt cattcatcag taacacgtca cggcactgct cggcttcgtc gagatacgag
    14941 gtactccaga ggattaacat cccttcgcct gccagctcat gcaccatctg ccacagttcg
    15001 cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac tttcggttcg
    15061 cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag cttgcccgcc
    15121 aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa agtttgctta
    15181 cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc catcaccgtg
    15241 agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg cagcgcgccg
    15301 tcgtttttga ttggatcaaa gccaatcacc gtggcattgc cgctgtcggg tttcagtaat
    15361 cccgccagca tccgcattag cgtggtttta cctgcaccat ccggccccac cagccccgtc
    15421 acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc catgcccgga
    15481 aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg cctcgttacc
    15541 gaattgcacc gtcaccggca ttccctggcg taacgcatca tcggcgtcgg tcaccacaat
    15601 acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt tcggggtaaa
    15661 ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg ggcgaccatc
    15721 ggtataaagc agcactttgc gccccggctg ggcctggtca agataccgtt catcaacata
    15781 agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac cttcattgag
    15841 gaccgtgcct ggctccaccg cgcgcgttaa cagcgtgcca tcagacgggg ctatcaacgt
    15901 tgagtcctgt aaattcaact ccgcctgcgc cagttgcgcc tgcgcctgtt cgaggctggc
    15961 tttcgcctgg gcgatgtcct gttcacggtt accggaacgg tactgacgca atttatcctg
    16021 tgctgatttc agcgttgcct gcgcctggtc gcgcgaggag cgggcatttt ccaggtcatt
    16081 tgccgaaata gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt tctgcgcata
    16141 gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt cttcatcgcg
    16201 atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg ctttcgcctg
    16261 catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc ccgctttgat
    16321 agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga aactaagatt
    16381 caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc ggctttgata
    16441 ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtt cgatcacgac
    16501 aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag gtcgatatga
    16561 caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt ccagccggta
    16621 cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat caacgcatgg
    16681 gtatgaagga tcatgcgggt gtcattggcg tcgctgccgg tccaggcggc aatcaggcgt
    16741 gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta ggctgccgtg
    16801 ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc ctgggtgagc
    16861 agcttaatca tgttcctgca ggcgcgaagg agcagttcac ggatggcagc ccgatcaggc
    16921 tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg ctcgccaata
    16981 aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag
    17041 gtgatggcag cgatattctg cccggcttgg gcggctatct cgcgggtggt ggcgttcatt
    17101 ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc ctgttcaccc
    17161 ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat gattgattaa
    17221 gattatgact ccatatggga gttgtccagt atggctaaga attttagcta cgccagtcac
    17281 agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctcgctc atgacattgt
    17341 ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa cggtcggggc
    17401 ggttcggagt agtcatgtct ttcgattatt tgggtttaag ccctgatatc ctgcgcgccg
    17461 ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc cctgcggtgc
    17521 tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca gcgggcttta
    17581 cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg cgtcgtccgg
    17641 tacgtgcgct cattcttacc ccgacccgtg aactggcggc gcagattggc gaaaacgtcc
    17701 gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggtggt gtgagtatta
    17761 acccgcagat gatgaaactg cgtggcggcg ttgatgtgct ggtggcaacg ccgggacgtt
    17821 tgctggacct ggaacatcag aatgcagtga agctggatca ggttgaaatc ctcgtcctcg
    17881 atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc gtgttaacaa
    17941 aactacctgc gaagcgccag aacctgttat tctccgcgac cttctctgac gatattaaag
    18001 ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc cgcaataccg
    18061 cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc
    18121 tgtcgcacat gattgggaaa gggaactggc agcaggtgct ggtgtttacc cgtaccaaac
    18181 acggcgctaa ccatctggct gaacagctca ataaagatgg catccgtagt gcggcgatcc
    18241 acggcaataa atcgcaaggt gcgcgtactc gtgcgctggc tgattttaaa tcgggcgata
    18301 ttcgtgtact ggtggcaact gacatcgctg cgcgcggcct ggatattgaa gagctgccgc
    18361 acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccatcgt atcgggcgta
    18421 ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat gaacacaaac
    18481 tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg attccgggct
    18541 atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag caacgtggcg
    18601 gcggcggtcg tgggcaaggt ggtggtcgcg gtcaacagca accacgccgt ggggaaggtg
    18661 gcgcaaaatc tgcaagcgcg aaacctgcag aaaaaccgtc tcgccgcctc ggcgatgcca
    18721 aaccggcagg cgaacaacaa cgtcgccgcc gtccgcgtaa acctgccgct gcgcagtaat
    18781 cttttatgcc gggctatgcc cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc
    18841 gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg cttttgtagg
    18901 ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc agcaatttga
    18961 gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg ttacttctct
    19021 atagccaatt gttctcgcaa ctccattaaa aggtagccca gtcgattctt gcccttacca
    19081 ttaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt tgcgggcgcg
    19141 gttgccagca agagcacacg cagttctgca tgctgttcga atttagcacg aagtgcttta
    19201 cgcatcacct gttctttaac cgactcccag tttttacgaa gtggcttgga acgattacgc
    19261 cccatgcgag cggcgaccat gggggaagaa acccgacgga tctcttcgcg gtatttttca
    19321 tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg gccaggtttt cccgtcaacc
    19381 ttgatgggcc aggcggcaaa gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa
    19441 tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg
    19501 cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat tacccctgaa
    19561 aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc tcatggcatt
    19621 aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg aacagatccc
    19681 cgactttatt ccccgtgtgc cgcagcggca gatgattgcg gacgtcgcca aaacgctggc
    19741 cggagaagaa ggccggcatc tggcgattga agcccccacc ggcgttggga aaacgctctc
    19801 ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac
    19861 cgccaacgtg gcgttgcagg atcagattta cagcaaagat ttaccgctgc tgaaaaagat
    19921 cattcccgat cttaaattca ctgccgcttt cgggcgtggg cgctacgttt gtccgcgtaa
    19981 tctgacggcg ctcgccagca ctgaacccac gcaacaggat ctgctggcgt ttcttgacga
    20041 cgaactgacg gcaaataatc aggaagagca aaaacgttgt gcgaagctga agggcgatct
    20101 cgacacttat agatgggatg gtctgcgcga tcatacggat atcgttatag atgatgatct
    20161 ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt actactatcg
    20221 tgaatgcccg ttttttgttg ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa
    20281 ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct
    20341 gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg cgctggagat
    20401 gagcgctgaa atcaccgcgc cctggtatcg actacagctg gacttgttca cgaaactggt
    20461 cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga tccctgaacg
    20521 tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca acattctcaa
    20581 tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg aactgcctga
    20641 tgaagtgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc gtggtctggc
    20701 ggagttattt cttaacgatt taagtgagaa aaccggcagc catgacattg tacgcctgca
    20761 tcggttgatt ttgcagatga accgcgcgtt ggggatgttc gaggcgcaaa gcaaactctg
    20821 gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg cgacgcggga
    20881 agagcgcgaa gggcaactgc atctctggtt tcactgcgtg gggatacgtg tcagcgatca
    20941 gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg caaccttgcg
    21001 ttcgctgaat agtttttcgc gtttgcagga gatgagtggt ctgaaagaga aagcgggcga
    21061 ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc
    21121 ccggatgcgc gttgagcctt ccatcgacaa cgaagagcag catattgccg aaatggcggc
    21181 ctttttccgt aagcaggtgg agagcaaaaa acatctcggt atgttggtac tgtttgccag
    21241 cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgtctga tgttgctggt
    21301 tcagggcgat cagccgcgtt accgtttagt tgaactgcac cgcaaacgcg tcgccaacgg
    21361 tgagcgcagc gtgctggtgg gcttacagtc atttgccgaa gggcttgatt tgaaaggtga
    21421 tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca gccccgtggt
    21481 gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct
    21541 gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa gccacggttg
    21601 ctggggcgaa gtggttatct acgacaaacg cttgctgacc aaaaattatg gcaagcgact
    21661 actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag gtatagttaa
    21721 aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa
    21781 ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga aaaaccacgc
    21841 gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct catatgctca atggtgaagt
    21901 ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt atcaaaggag aaggggaagc
    21961 agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgccagc
    22021 aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggccaacct
    22081 gacgccgttg ttggcgattc tcctgcacaa actcggtttt cctgtggtgg ttcacggggt
    22141 tagcgaagat ccaacccgcg tgctgactga aaccattttt gaattgatgg gtattacgcc
    22201 aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc
    22261 agtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt
    22321 gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgcggcgct
    22381 gcgtctttcc agcgtttcgc atccggaata tattggacgc gtcgcgaagt tctttagcga
    22441 tatcggcggg cgggcgctac taatgcatgg tactgaaggt gaagtgtatg ctaatccgca
    22501 gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca
    22561 ggacactgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg
    22621 gattgagcgt tgccttgctg gcagcgaacc gattcccgaa tcgctgaaaa tccagatggt
    22681 ttgctgcctg gtggctacgg gtgaagcggc aactatcagc gacggcctgg cgcgcgttaa
    22741 tcaggcattt taattctttt ctcagccgga tgagccatgt catccggctt ttaccccacc
    22801 gcaatatgaa attcctgcat ctttattgac cttcccacgc ccggcgtgca gcataaaaat
    22861 acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct
    22921 cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa
    22981 gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttcacatggt
    23041 attggcatga tcccaagcta tgtacgctcc tggagtcagg ggcacctgca aattaaccat
    23101 catgccaaaa ccgttaaaga ggcgggggcg gcggtcacgc tcgatggcga tcgcgcattt
    23161 ggtcaggtcg cggcacatga agcgatggcg ctggggattg agaaagcgca tcagcacggt
    23221 attgccgccg tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag
    23281 cagtgtgcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg
    23341 gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atccgttctg tgtggttttc
    23401 cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc
    23461 aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt
    23521 aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg ttcgctgttg
    23581 acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg
    23641 ctttccggcg gtaaaacgac gcatcaggaa acgttacaaa ccagtcccga tgccattctt
    23701 aactgcatga ccactatcat catcaacccg gaactcttcg gcgcgccgga ttgtaacgcg
    23761 cagaccgaag cctttgccga gtgggtgaaa gcctcgccac atgatgatga taagccgatt
    23821 ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcagaagca ggggattcca
    23881 ctggatgcgg gaagctggca ggccatttgt gatgcagcgc ggcagattgg tatgccggaa
    23941 gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg
    24001 gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca
    24061 aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc
    24121 ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg
    24181 gcgtaaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt
    24241 attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg cggctttctc
    24301 agccagtttc gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat
    24361 tttattcatg ttctgtgcct gggatgccgt tatcggttcc gccgcgaaca cgccaaatga
    24421 cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc
    24481 gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt
    24541 acgagagata aaatcgaaga gaattgaccg ccttgttc
//
LOCUS       DAZOZD010000041        24343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOZD010000041 DAZOZD010000000
VERSION     DAZOZD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.041"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            230..367
                     /locus_tag="ACHUPB_001326"
                     /pseudo
     CDS             230..367
                     /locus_tag="ACHUPB_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(475..1710)
                     /locus_tag="ACHUPB_001327"
     CDS             complement(475..1710)
                     /locus_tag="ACHUPB_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0758191.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1857..1933)
                     /locus_tag="ACHUPB_001328"
     tRNA            complement(1857..1933)
                     /locus_tag="ACHUPB_001328"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1897..1899),aa:Pro,seq:tgg)
     gene            complement(1976..2062)
                     /locus_tag="ACHUPB_001329"
     tRNA            complement(1976..2062)
                     /locus_tag="ACHUPB_001329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2026..2028),aa:Leu,seq:cag)
     gene            complement(2083..2158)
                     /locus_tag="ACHUPB_001330"
     tRNA            complement(2083..2158)
                     /locus_tag="ACHUPB_001330"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2123..2125),aa:His,seq:gtg)
     gene            complement(2217..2293)
                     /locus_tag="ACHUPB_001331"
     tRNA            complement(2217..2293)
                     /locus_tag="ACHUPB_001331"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2257..2259),aa:Arg,seq:ccg)
     gene            complement(2394..3779)
                     /locus_tag="ACHUPB_001332"
     CDS             complement(2394..3779)
                     /locus_tag="ACHUPB_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0758192.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3970..4710)
                     /gene="wecG"
                     /locus_tag="ACHUPB_001333"
     CDS             complement(3970..4710)
                     /gene="wecG"
                     /locus_tag="ACHUPB_001333"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0758193.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4713..6065)
                     /gene="wzyE"
                     /locus_tag="ACHUPB_001334"
     CDS             complement(4713..6065)
                     /gene="wzyE"
                     /locus_tag="ACHUPB_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0758194.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6062..7141)
                     /gene="rffT"
                     /locus_tag="ACHUPB_001335"
                     /gene_synonym="wecF"
     CDS             complement(6062..7141)
                     /gene="rffT"
                     /locus_tag="ACHUPB_001335"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0758195.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7138..8388)
                     /gene="wzxE"
                     /locus_tag="ACHUPB_001336"
     CDS             complement(7138..8388)
                     /gene="wzxE"
                     /locus_tag="ACHUPB_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0758196.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8390..9520)
                     /gene="rffA"
                     /locus_tag="ACHUPB_001337"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8390..9520)
                     /gene="rffA"
                     /locus_tag="ACHUPB_001337"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0758197.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9525..10199)
                     /gene="rffC"
                     /locus_tag="ACHUPB_001338"
                     /gene_synonym="wecD"
     CDS             complement(9525..10199)
                     /gene="rffC"
                     /locus_tag="ACHUPB_001338"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0758198.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10177..11058)
                     /gene="rfbA"
                     /locus_tag="ACHUPB_001339"
     CDS             complement(10177..11058)
                     /gene="rfbA"
                     /locus_tag="ACHUPB_001339"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0758199.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11077..12144)
                     /gene="rffG"
                     /locus_tag="ACHUPB_001340"
     CDS             complement(11077..12144)
                     /gene="rffG"
                     /locus_tag="ACHUPB_001340"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0758200.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12144..13406)
                     /gene="wecC"
                     /locus_tag="ACHUPB_001341"
     CDS             complement(12144..13406)
                     /gene="wecC"
                     /locus_tag="ACHUPB_001341"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0758201.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13403..14533)
                     /gene="wecB"
                     /locus_tag="ACHUPB_001342"
     CDS             complement(13403..14533)
                     /gene="wecB"
                     /locus_tag="ACHUPB_001342"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0758202.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14589..15635)
                     /gene="wzzE"
                     /locus_tag="ACHUPB_001343"
     CDS             complement(14589..15635)
                     /gene="wzzE"
                     /locus_tag="ACHUPB_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0758203.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15647..16750)
                     /gene="wecA"
                     /locus_tag="ACHUPB_001344"
     CDS             complement(15647..16750)
                     /gene="wecA"
                     /locus_tag="ACHUPB_001344"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0758204.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16990..18249)
                     /gene="rho"
                     /locus_tag="ACHUPB_001345"
     CDS             complement(16990..18249)
                     /gene="rho"
                     /locus_tag="ACHUPB_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0758205.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18334..18435)
                     /gene="rhoL"
                     /locus_tag="ACHUPB_001346"
     CDS             complement(18334..18435)
                     /gene="rhoL"
                     /locus_tag="ACHUPB_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0758206.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18576..18905)
                     /gene="trxA"
                     /locus_tag="ACHUPB_001347"
     CDS             complement(18576..18905)
                     /gene="trxA"
                     /locus_tag="ACHUPB_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0758207.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19036..20301
                     /gene="rhlB"
                     /locus_tag="ACHUPB_001348"
     CDS             19036..20301
                     /gene="rhlB"
                     /locus_tag="ACHUPB_001348"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0758208.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20435..21919
                     /gene="gppA"
                     /locus_tag="ACHUPB_001349"
     CDS             20435..21919
                     /gene="gppA"
                     /locus_tag="ACHUPB_001349"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0758209.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21966..23987)
                     /gene="rep"
                     /locus_tag="ACHUPB_001350"
     CDS             complement(21966..23987)
                     /gene="rep"
                     /locus_tag="ACHUPB_001350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0758210.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24204..>24343
                     /locus_tag="ACHUPB_001351"
     CDS             24204..>24343
                     /locus_tag="ACHUPB_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758211.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6128 a         6665 c         6007 g         5543 t
ORIGIN      
        1 taaaaaagca ttaataatca acgcaatata ataaacccgc cttacatatc aactgcgcca
       61 gaggtaggat tgaaaacgct ctcctgattt tccaattcat tttctggata aataaataat
      121 ttatttttgt cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca
      181 atgcttaatt acgtcatcat tttgatgaca tgaaaccgga gcattttgca tggaattttc
      241 gttttcaccc aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc
      301 agcagatacc ccgtcaactg ccaccgcacg caaaggcttt gccggatacg atcacccaaa
      361 ccagtatcca cagaacgctg caatttattg aatttgcacg atcatgtagg ccggataagg
      421 cgtttacgcc gcatccggca atcaaccgca ggcggccaca aaatggccgc ttttttactt
      481 attcaccacc agcaaatgtg cctgcataat gtcactggcc gggcgaccgt gcgccagcaa
      541 atcagccatt gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca
      601 caaataattc agccccggtt tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc
      661 tccccagcac gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt
      721 atcttcacca aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctgatg
      781 catattcccc agccgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt
      841 gtgctcaaca atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc
      901 accgacaaaa ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg
      961 tttgatccag tggtcgaata tcgccaccag aaattcacca aattcatcgg ggcgcaccga
     1021 ccattccgtc agttccccct gaatatcacc aggcgcatgc aatttcagtc catcgcgggc
     1081 agctgtttca tcagccagac gctcgaccac cggaataaac tggatgaatt cgactcccgc
     1141 atcgcacaaa aaatcataaa cctgcaacgg ttgctgcgcg ctggtgcggt tgacgcagac
     1201 cagcacgtta tagtcgacat gatgtttttg cagcagcgtc agggcacgca tcaccagctt
     1261 atgcgttggt ctgccacctt tggtcacgcg atattgattg tggatctcag ccggaccgtc
     1321 cagcgataac ccaacaagaa aatgattttc tgccagaaat gcacaccatt tatcatcgag
     1381 cagcacgccg ttagtctgga agctgttact tatcttcctg ccagcaccgt atttcgcctg
     1441 taactttacg gcacggcggt aaaaatccag cccgagtaac gttggttcgc cgccctgcca
     1501 ggtaaaagcc acttcgtttt gcggttcgct ggcagcgata tagtgacgga catacgcttc
     1561 cagcgtgtca tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag
     1621 ataaaaacag tagtcacagt tcagattaca atcggaacca ctcggtttcg ccatcacatg
     1681 aaaagcacgc gttggaacct gttgcagcat ggtcactcct tgcctgaagt catccttcaa
     1741 tacgttaagg gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag
     1801 ggagggtgac gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt
     1861 cggcgagaga ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct
     1921 gcgctactcg ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg
     1981 cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt
     2041 ctaccaattc cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat
     2101 tcgaacccac gacaactgga atcacaatcc agggctctac caactgagct atagccacca
     2161 ctacaaatct tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc
     2221 gcgcccgaca ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct
     2281 gagctacggg cgcttagcgc cgttgcgggg ggataatacg gacttcccac ccctctgtct
     2341 agtccctttt taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc
     2401 tccagtttat gcgctttccc gtggcgatta aggccaaaaa ctttataaat cgccgtcacc
     2461 gccagcataa agatgatgcc aacaaacagc gacatacgcg tatcttcatt aaagtacatg
     2521 ccgatcaaaa cgcaaatcag gaatgccatt gttacgtagt tggcccacgg gaacaggatt
     2581 gagcggaacg gatggctggc aatcgccgct ttatgtgcac gccgaaaacg cagctggctt
     2641 atcaatatca caaaccatgg caccatcccc ggaagcacgc tggcactgta gacgtagaca
     2701 aacacacgct gcggattggg aatgatgtag ttcaggcatg agccaattag cagaatagca
     2761 atagatactg ccacacccgc aactggtacg ccgtgacggg aaactttcgc catcgccgcc
     2821 ggtaactgac ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta catgccgctg
     2881 ttacagccag agagcgcagc cgtcagcacc acaaagttga taatgcccgc cgctgcggta
     2941 ataccgattt tggcaaaagt cagtacgaac gggctgccgt tgctgccaat ttcattccac
     3001 gggaagatgg tgacgataac gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc
     3061 actttgccta cagcactgcg cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg
     3121 ccaatcagct ccacgccctg gtaggacgcc accacaatac acagcgcggt caggaaccct
     3181 ttccagccac ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc aatcgactgc
     3241 ccgccattgc caaagccaaa gaaaatcacg cccaggccaa tgacaatcat cacgataatc
     3301 gtggtgactt tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc
     3361 agattcgcca acgccaccag cgccaccgcg atcaatgcag gtatccactg cgccatctcc
     3421 gggaaccaga actggacata aacgccaatg gcggtgattt ctgagatccc caccgccatc
     3481 cacataaacc agtaagacca ggcggtgaga taaccaaaaa acgggctcat ataacgatgc
     3541 gcataaacgg cgaacgaacc ggtaacaggt tcaaggaaca acatttcgcc cattgaacgc
     3601 atgatgaaaa agacgaacag cccggcgatg atataggcca acaatacgga tggcccggcc
     3661 catttcaggg tactggcggc ccccataaac aggccgacgc caatagtgcc ccccagggcg
     3721 atgagttcga tatgtcgagc ttccagccca cgctgtagct ctggtttgtt atctgccata
     3781 aatcctcgtg ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat gggtacatcg
     3841 agtgttgcga atgttttcgt aattcaggag aaatggcaaa taaagcatta aaaatttgaa
     3901 tgctttgtgt aataaaaaag cagacaggcg acggagtgac cactccgtcg ctttacaaag
     3961 agaggaaaat cagaggttgc cggtgtagtg ccagcgtaaa taacgcagca aacgaagctg
     4021 acgcttaatg cggctcggct gcgaaagcag gcggtagagc cactccagtc ccagcgtttg
     4081 ccagattttc ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc
     4141 catatacagc gcatctggat gcaccagacg gcagtcgcgc atgaagatct cctgctttgg
     4201 cgatcccatc gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc
     4261 ctgacgctgc tcgggtttaa aataaccatc ctgactgcca acgatattca cattccattg
     4321 gttgcgcagt ttagcttcag tttgcgccag cacttcaggt ttaccgccca caagaaatac
     4381 cggcgtccct tctttgcctg cgcgcgccat cagctcttcc cagagatcgg caccggcaac
     4441 gcgggaaacc tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc tgatgccatc
     4501 cgcatattta aattcggcag cgttaattaa ctccctgacc tcggcgttat cttcaatggt
     4561 cagcattttt tcagcattaa tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa
     4621 cagataatcg agggcgtgct gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt
     4681 ataggtcggt gccgtggtgt tgttattcat tgttatcctt caacctgcgt ccggagcgat
     4741 gattttgtac gtttatgaat gagtccggcg ctttcaaaaa gccagtacag cagttttgcg
     4801 atcatcagac atgcgccgaa gaccacgata aaaaagacta cgcgtgagac aaacgaatcc
     4861 agcccttcac gcgccagcac gatcatattg aagatcgccc caaagcagaa actgtgcaat
     4921 atcgcagcct tatagcgatt aggctcgcgg ttgcccagct catacagcca gtcgaaccat
     4981 ttgatgatca gaccaaccac gatcgccccg agcgggatga acaacgcgcc gcccatcacc
     5041 accagtgagc ctataagcgt aggcgagatc gccagtccgg agtggttatt cagcacttcc
     5101 caggtaaagt agttggctga gttcagcacc atactcgggc gacccggcca cagccaggaa
     5161 gggataaaga catagaaatc gcggacaatt ggagccaggc cctggaagtc gatgttgtcg
     5221 tagttctgca acagcaacgc cagattctcc cacggggaga aggtgtcgcg agtgagatag
     5281 agaaacgtat agaacgcttc atcgccgctc acattcattc catagcgttt tagcgccagc
     5341 cagaacatgc caacaatccc cagcacgccc gccgccgcca gcatccacaa cgaaatccag
     5401 ccgcgaataa tgccaataaa caggaagata gcgaatgcga tgatgatatt ggcgcgagtg
     5461 ccgccgacaa tcatataagt cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc
     5521 cacgctttgc tgtcctggcg cagaaagtag accaccagca tcgccgggat gaaaaagtaa
     5581 aagaagcgtt ttaacgccac gccggagact tcactggaga agatctgact gtaggagtta
     5641 agccggaaca gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac cagcgcgata
     5701 cccatcagga tcacccacgt aagattggtc tccacgcggt tcatggtaaa cagcggacgg
     5761 cgcggtacat cagcaacgcg tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg
     5821 tagaagcagc ccgcagaaag caacgcctgc aacaagattt ctggcggcgc gacaccaaca
     5881 tcaaagcgaa ataccagcac gctggtcagc gggaagccga agaaaaaggt gagcaaaaac
     5941 agcaatgaaa agaagacatt gaagttaaag cgcacacggc gaaactcaaa ccaggtcagc
     6001 gtggcaataa acagcgtgca gagcagccag accacaaaca ggccactgaa ttgcagcaga
     6061 ctcatgcgac ctccccggcg gcaatagcca acgcccgctg ccagccttgt agatagttag
     6121 ggctaaagaa ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc gcttcacgca
     6181 caatatcctc gttgagatcg tcggtagtaa acagcaccgg caaatgctgt tccgtcatat
     6241 cctgccagaa cggattttcc cggttaagca cacaaggaat gcccgcctga atcagtaagc
     6301 aaagcgtacc aatgccctgc tggcgggcaa aaataaagta accaagatcg cactgacgaa
     6361 gtagcgccag ataggcgtca aattccagtt tttcgctcaa aatttgcaga ttttcttcgc
     6421 tgaataactc cagccccgcc tgacgaactt cctcaatgta cgcttcgtta ttaggcggat
     6481 atcccatcgg caccaccact tttaccgtat cgccaaattg ctgatgaacg gcgcgcaagg
     6541 cagcaatatg gtcattgctg cggtcaccgg agttccccac cagaatggtc attttccctt
     6601 cacgttgccg atcattcgcc atcgtattga gagaagggtc catccgcgtc gggaagtaca
     6661 gcagttcgcc ccgcaccttt ggatgcgttt tggcaaaaaa gctcaaatca ccgcgggtgg
     6721 caaatacaca gccgactcgc ttttgcgcca ggcgacgtag cgggtaaaaa agcttatatc
     6781 tcaagccact ggaaagctca tacaggtctg ccccccagat atgccagaaa aactggctgg
     6841 gcttaatgcc accactcagc agagccagcc acagtgtggg attgaactga ccgtggaaaa
     6901 aaaatcgctg ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc agcgattttt
     6961 tcccagggaa aaattgcaca gaaagcgccg gacagctatc acttaagccg tcgtccttgc
     7021 caacaaccat aaactcgcgc gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc
     7081 gcaaaacggt tcggttatgg tgagggatat ccgatcccag tacgtgaatc agtacagtca
     7141 tgcccgccta cgccagagta aaaacacgcc acaacaaaga gaaaaataga cgatataagt
     7201 tgccatatat gcctgcgccg cgcccagtgc accatgcgca gggattagcc agtgggcaaa
     7261 taccatcaat aaagtgaact ggctgacttc cgccagaata taaaaccgca gtgacgcttt
     7321 ggcgatcacc agataaccaa agacataagc gcccactttt aacacatcac ccactaactg
     7381 ccaggcaaag agatcgcgca tagcggtaaa tttattcgac aacagcaacc agatagcaaa
     7441 atcacgcagc aaccagacgg taaaactcgc cgccgccact gccggtaaga cgaatttcag
     7501 cgatttaacc acttcccggg tgatatcgcg cttttccgtt agccgcgaca acgtgggcag
     7561 caaatatacg ctgaacgatg ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac
     7621 cccttgccag atccccactt catcccagct atactgcgcc gccagcagtt tacgcatcat
     7681 gatgtaagca acaggcaagg tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa
     7741 ctgccctgcc agaccgttat cccagctggg tttcagatag cttaacggga tgacaccacg
     7801 tttgatcaac atgatggcgg caggaatcac caccagcgcg ggaatcagcg ccagacccag
     7861 caacgcccct tcataaccgc ccaaacggta actgacgtag tacgcgagaa cgccaatcaa
     7921 gctgccgaca atcagcgata acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc
     7981 cagtaacagg ttgccccagg cgatccccat ttgcaccagc gccactaaac gcaccagccc
     8041 ctgatagtcg gtattaccaa acagtccctg gctgattggc gcagctgcca gcacaaaaac
     8101 cagcgccatc agcgtagaga aaccaagtac catcgctgat gaagtgccga ccacgcggcg
     8161 cagctgttgc ggattatcat ggtactgggc aacgtatttg gttacaccgt taaagatgcc
     8221 agctccggca agcacgccga gcacggtaat caactggcgg aaatttgccg ccagcccaag
     8281 ccccgccgga ccaaatgaca cagccagcaa cttaccgacc agtaacccgg caccaatctt
     8341 gaccagtgta ctggccgccg tccacaagga cgcttttgcc aacgacatat caggagaagt
     8401 agttcaacaa agtcgcaatt accgtacgct gattgacggg cgacaggttg tagaacagcg
     8461 gcaggcgcag caggcgctcg ctctctttgg tggtgtagcg atcttcaccg tggaactcac
     8521 caaagcgttc ccccgcaggg caaccgtgca gcggaatgta atggaacacc gccatgattt
     8581 ccgcttcttt cagaaagtta atcaacgcgc tccggtcatc aatatcccgc agtttaatgt
     8641 agaacatatg cgcgttctgc acgcagccat cgggaatcga cggcagctcg atacgcccgg
     8701 ctttcgccag aggcgctaac gcatcgtagt agttttgcca cagcgccaga cgttgctggt
     8761 tgatacgatc cgctgcttcc agttgcgccc acaggtacgc agcttgcaga tcggacatca
     8821 aatagctgga gccaatatcg cgccaggtat atttatcgac ctgaccacgg aagaactggc
     8881 tgcggttagt gcccttttca cggatgatct cggctcgttc gattaacgct ttatcgttaa
     8941 tcagcgtcgc gccgccttca ccacccgccg tatagttttt ggtttcatgg aagctaaagc
     9001 agccaatatg accaatggtt cccagtgcac gccctttgta agtggacatc acgccctgag
     9061 cggcatcttc taccacaaac agattatgct ttttcgccaa cgccataatg gtgtccattt
     9121 cgcaggccac acccgcgtaa tggaccggca cgataacgcg cgttttgtcg gtgatcgccg
     9181 cttcaatcag cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca aaaacgattt
     9241 ttgcgccacg cagcacaaag gcattggcgg tggagacaaa ggtgtagctc ggcatgatca
     9301 cttcatcgcc aggctggata tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg
     9361 acggcgtcag taacactttg gcgctgccaa aacgttgctc cagccactgc tggcagcgac
     9421 gggtaaaacc gccatcgcca cacagtttgc cgctacccat tgccgactgc atatagtcga
     9481 gttcggttcc caccaccggc ggtgcgttaa atggaatcat gtgatcacct gtataaccag
     9541 tacgcggtgc tttctacatt cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg
     9601 cccatttggg tcgccacccg caaagttgtt ttaccgcgag catacgccca gtttagcgcc
     9661 gtttgcatca gctcagcacc tgcaccgcgt ccagccagca ggccaattcg cgcatctgtc
     9721 gcattgagtt cccgtaaaga gacataaccg cgaataccgc cggacgccgc acgtaaaatc
     9781 agacattgat gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc ataaaagcgt
     9841 ccgctggcgt caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg
     9901 gctaactgac gtaatgcggg aatatcggtc tcttgtgcca ctacagcacc actatcactg
     9961 acattgttca cgggtagtgc caaatcaact tcaccttcta ccagggagaa tcccagctgt
    10021 tgcagggcat ccagttcacc cgtatttgat gccgcaattt tggcctgcac ccgtgaccac
    10081 ggcgctaaca cgtctggcgt caggagctgt gcttcagacg taatgcgcac gatggcgctg
    10141 ttaacaccaa agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg gacgggcacg
    10201 aagtaactcc agcagatatt ggccgtagcc agttttcgct aatgaactgg cagcacgctt
    10261 cacaccctcg tcatcgagcc agccgttacg ccaggcgatc tcttccaggc aggcaatctt
    10321 aaagccctgg cgtttttcta ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg
    10381 agtgccagtg tccagccagg caaatccgcg cccgagcagt tcaacggtca ggttgcccgc
    10441 ctcgaggtac atctggttga tggaggtaat ctccagttca ccacgctccg acggcttcac
    10501 ctgctttgcg tactccacga ctttactgtc gtagaaataa agcccggtca ccgcccagtt
    10561 tgacttcggc tgttttggct tttcttccag cgagatagcg cggaaattgt cgtcaaactc
    10621 caccacgcca aagcgttccg ggtccatcac ctgatagcca aataccgtcg ccccttccgt
    10681 gcgcgccgca acatgacgca gcttcggact gaagccctga ccgaagaaga tgttatcgcc
    10741 cagcaccaga caagaaggtt caccgttgag gaaggtttca ccgatgataa aggcctgcgc
    10801 cagaccgtcc gggctgggct gttcggcgta ttccagctgg ataccgaact cactaccatc
    10861 gcccagcagg cgctggaaat aacctttatc ttccggcgta gtgatgatga gaatttcgcg
    10921 gatatcggcc aacatcagca ccgacagcgg atagtaaatc attggcttat cgtaaatcgg
    10981 caacagttgc ttcgatacgc cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc
    11041 caggataata cctttcatgt ttgcctccgc tgaaaattag cctttcagac ctaaacgctc
    11101 gccctgatag ctgccgtcct gcacctgctt ccaccagctt tcattagcca gataccactg
    11161 caccgtttta cgcattccac tttcaaaggt ttcctgtggc agccagccaa gttcacgggc
    11221 aattttcgaa gcatcaatgg cataacgcag atcatgcccc ggacggtcag cgacaaaggt
    11281 gatcaagtca cgatagtgca ccacgccgtg cggcttgttc ggagccagtt cttccagcag
    11341 ctcgcaaatg gtttccacaa catcgagatt cttacgctcg ttgtgaccac caatattata
    11401 ggtttcaccg actttcccgg tggtcgccac gcaatacagc gcgcgggcgt gatcttccac
    11461 atacagccag tcacggattt gctgcccgtt gccatatacc ggcagcggtt tacccgccag
    11521 cgcgttgagg atcatcagcg ggatcagttt ttccggaaag tggtaaggac cgtagttatt
    11581 cgagcagttg gtgataagcg tcggcagacc gtaggtccgc agccaggcgc gcaccagatg
    11641 gtcgctgctg gcttttgacg cggaataagg gctgctcggc gcatacggcg tggtttcggt
    11701 gaagaaatca tccgtcgagt gcaggtcacc atatacttcg tcggtggaga tatgatgaaa
    11761 acggaacgct gattttttat cttccgtcag cgtattccag taagcccgcg ccgcttcaag
    11821 caatgtataa gtcccgacaa tgttggtttc aataaacgct gccgggccgt caatagaacg
    11881 gtcaacatgg ctttcggctg ccagatgcat gacacagtct ggctgatgct cagtgaatac
    11941 gcgtgccagt tctgcccgat cgcagatatc aactttctca aaggcaaagc gctcgctttg
    12001 cgcgaccggt gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga ccactaccac
    12061 cgcgtcgctc gtttcgttga tgatataacg caccagcgcc gagccaataa acccggcacc
    12121 acctgttatc agaatttttc tcatcagcgc cagactcctt tggcatcgac gacatactgc
    12181 tgatggacat tgtcgccatt gataacttta aactgactat gatcgaccag catcaccagc
    12241 acgtctgccg ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt
    12301 ttcggcaact ggtggatgtt aggctcaaca accagagttt cgccgctatg ccactgggcg
    12361 atcagttcag cgatttccat cgccgggctt tcgcgcaggt catcaatatt cggtttaaac
    12421 gccagaccaa agcaggcgat tttcagttca ctggcgcgtt tatcggtagc cgccaggcaa
    12481 tcagccaccg ccgctttcac ctgatcgata acccagaacg gtttgtgatc gttcacttcg
    12541 cgcgcggtac ggataagccg cgcctgctgg gggttctgtg ccacgataaa ccacggatca
    12601 acagcaatgc agtgaccgcc cacgccaggg ccaggctgaa gaatattaac gcgagggtga
    12661 cgattcgcca ggcgaatcag ttcccagacg ttaatcccct gatcggcaca aatcagcgac
    12721 aattcattag caaaagcgat attcacatcg cggaagctgt tttcggtgag cttacacatt
    12781 tccgccgtcc gcgagttagt gacgacacac tcaccttcga ggaaaatttt gtacagttcg
    12841 ctggcgcggg tcgaacaaac cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc
    12901 tcgaccataa cctggcccgg taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc
    12961 tgctcgccca cctgctgtgg gaaagtgaga tccggacgca tctctgctaa ccattctgcc
    13021 atcttctcgg ttgaccccac cggcgaggtg gattcaagga tcaccagcgc gccttttttc
    13081 agcactggcg caatggagcg agcagccgat tcaacgtagg tcatatctgg ctcatgatcg
    13141 cctttaaacg gcgtgggtac agcaatcagc caggcatccg cttcaactgg cgtcgtgctc
    13201 gctcgtaaaa aaccgccttc tacggcagtt tttactacgc tcgccaaatc aggctcgacg
    13261 atatggattt cgccacgatt gatggtatca accgcgtgtt ggttgatatc gacaccaatc
    13321 acctgttttt gccgtgaggc aaacgctgct gctgtaggca gcccgatgta acccagtccg
    13381 ataacagaaa tggtcgcaaa actcatagtg atatccgatt attttttaac gcttccagaa
    13441 tgcgagagca tgcctgacca tcaccatacg ggttatgggc gcggctcata gtttgatatt
    13501 cgttttcgtc ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta tccgtgccta
    13561 ccagacgcac cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg cgcatcacca
    13621 gcaccggttt ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca
    13681 gccaggcgtg gttcatcagc cagacaaacg gtaaatactc ctggggatcg atcagaatga
    13741 catttttcac atgccccaga atgcgattga ccggctctct gacgttcggg ttgagatgca
    13801 ccggatagac aatctggatg tcctggtgcg tggtggcaat gtctgccaac gcgtggcaga
    13861 tttcttcaaa gccacgaccg aaactttcac gcctgtgacc ggtcaccaga atcatctttt
    13921 tatcggggtc gataaacggg taatttgccg ccagttctga acgcagcgta tcgctgctca
    13981 tcacctggtc acgcacccat aacagtgcat caataactgt attaccagta atgaagattc
    14041 ggctatccgc aacgttttca cgcagcaagt tttgccggga agtttcggtt ggagagaagt
    14101 gatacatcgc cagatgcccg gtcaatgtac ggttagcctc ttccggccac ggcgaataaa
    14161 gatcgcccgt gcgcagacca gcctcaacgt gaccaacagg aatacgctga taaaacgccg
    14221 ccaggctggt tgccagcgtc gtcgttgtgt cgccgtgaac cagcacgacg tctggtttga
    14281 actcggcaag aataggtttt agcccttcca gaatccgaca ggttatctct gtcaggccct
    14341 gtcctggctg cattatgttg agatcgtagt caggtacaat ggaaaagagt ttcagcacct
    14401 gatcgagcat ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt
    14461 ttgccaacgc atgcaccaac ggcgccatct tgatggcttc cgggcgcgta ccaaatacag
    14521 tcagtacttt cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg ctcaccgcag
    14581 cagtgttgct atttcgagca acggcgggtt aatgcgacac cagccccgat cagccccccg
    14641 acaatgcccc acataatcat caggaaggca cgacgtgggc tatcgcgttt taccggttct
    14701 tccggcgtac gcaaatagcg ataggtctga aaacgcggat ccagggttgg accaacattc
    14761 agggtgttta acatggcccg attctgatca tagtcgagat caaaggccgg accgacggcc
    14821 tgtaaatttt ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat ttctgaatca
    14881 ggtaattcct cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt
    14941 ttcagcgcct gttcaatact gttcatccgg cggtcgtaga tggctttcgc cacctcttcc
    15001 tgacgcttca cctgtgcttt catctggatg gtacgcgccg cccatgcgcc tttcagctca
    15061 tcattcagat ggctggctgc acgctggctg gcaaaagcaa catactgacg taacaggtta
    15121 ttagcgtcag gcgcggtttc agcaataagc ttcacgctgt cattgaccgc gcgggtaaag
    15181 tctccgggga taaactggat gttgttaatc atttcatcca gcaacgccgc atcggctttg
    15241 ctgttgccca ccatccgctg tttgtaatag tcggtttgca gccagaactc tctgcgggta
    15301 tcccacgagg ccagttgcat aacaaactct ttataggctt cgtccatgac cgatggttgg
    15361 tcggcagaag ccatgtttga acggacatcc aggttacgca aaaattgctg ctgcgagtaa
    15421 tatcccccca gcatattcac cgttggacga tcggtaatcg ccgtcgagct ccactcctga
    15481 cgagcaaaaa aagtatacgc cagcgcgatt aacgcaaatg ccagccccat gccaataatc
    15541 catagcttcc cagcccacaa ggtacgaaac aacccacgaa tatccagttc attttcagcg
    15601 tcttcggccg gtttcccagg cattggttgt gtcatcacat cctcatttat ttggttaaat
    15661 tggggctgcc accacgattt ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa
    15721 ctttccaggc acgcttaatg caatatccat agaggaagaa tgctagcaaa aagagcacca
    15781 gcatgaccca ctccgggaca aaatgagaat attctgccag cacgccaatg gaagcgagca
    15841 gtgccgcagc aagggtaatc agcacaaacg cctgacggga agtaaacccg gcacgcatga
    15901 tcaaatggtg aatatgctga cggtcaggag agaatgggct catgccttta cgcaggcgac
    15961 ggtacataat cgccaccata tccattagcg gaatggcgat tatccacaaa gcggtaaccg
    16021 ggctgatggg atgggttttg ccctgggtcg tttcgagcag gatccagata acggtaaaac
    16081 caatcagcgt actgcccgca tcacccataa agactttgta gcggcgaccc aggataccaa
    16141 ggttaagcat gatgtatggc aggatggcgg cgatcatcgc aaagcaccag attgcgaggc
    16201 tggtttgccc gtcgaaccac aaaatcatac cgattgctgc aaacgagacg caggacaacc
    16261 cgcccagcaa gccatcaatg ccatcaacca tgttgaacgc attaatggcc gcccagacgg
    16321 caaatagcgt caggaagtaa ccaaacggtc cgagcaccat ctcccaggag ccaaagatat
    16381 aacccaggct actgagataa agtttgccga acaccatcat aacaatgcca acagcggcct
    16441 gtatggtggc acggattttt acgctgatat caaaacggtc atccagcgcg ccaatgaaaa
    16501 caagcacacc ggcacaagcg agatagagag atgcatgcgg aatatagtaa tcgacaattc
    16561 cgaacgtgaa gcaaatccct gcgtaaaccg aaatcccccc aacgagaggt atcaatccct
    16621 ggtgacgttt gcggaagttt ggtttatcca ctaaaccgac tttttttgcc accttacggg
    16681 caaaaaacag aaacagtgtc gtgaataaaa aaatactgat gagatcagta ctcactgtca
    16741 gtaaattcac aatgcatgct ctcagagaaa attattagca gaagtataac cacgaagacc
    16801 tttattcaga agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt
    16861 gatatgcaaa ttgcataaca cgccagcaaa ttccaggaat tttcctaaca tcgccagcgc
    16921 ggcattaaga ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat
    16981 aagacaaatt tatgagcgtt tcatcatttc gaagaaatcg tcattggtct tggtcatggc
    17041 cagtttatta atgaggaatt ccattgcatc gatttcgccc atcgggtgaa tgattttgcg
    17101 caggatccac attttctgca gttcttcctg agtagtgagc agttcttctt tacgggtacc
    17161 ggaacggttg tagtcgatag ccgggaagac gcgtttttca gcgatcttac gagagaggtg
    17221 cagttccatg ttgcctgtac ctttaaactc ttcgtagata acttcgtcca ttttagaacc
    17281 ggtatcgata agcgccgtcg cgataatagt caggctgccg ccctcttcca cgttacgcgc
    17341 cgcaccaaag aagcgtttcg gacgatgcag ggcgttggca tccacaccac cggtcaacac
    17401 tttacctgac gccggaacaa cggtgttgta agcgcgcgcc agacgagtga tggagtcgag
    17461 cagaatgata acgtctttct tgtgctcaac caggcgtttg gccttctcga tcaccatttc
    17521 cgcaacctga acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt
    17581 taccagacgc tgcatctcgg ttacttcttc cggacgttcg tcgatcagca gaaccatcag
    17641 cacacaatcc gggtggttgt aagcaatgct ctgggcaatg ttctgcagca gcatggtttt
    17701 accggctttc ggcggtgcca caatcagacc acgctgacca cgaccgatag gtgatgccag
    17761 atccagtacg cgagcagtta aatcttcagt agaaccgtta ccacgttcca tacgcagacg
    17821 agaatttgcg tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg cgttttcagg
    17881 tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa tagcgttcac cttctttcgg
    17941 cgggcgaatc ttaccagaga tggtatcacc agtgcggagg ttgaaacggc ggatttggct
    18001 aggggaaacg tagatgtcat caggaccggc gaggtaggag ctgtctgcgg aacggaggaa
    18061 accaaatcca tcctgcaata tctccagtac gccatcacca aagatatctt cgccactctt
    18121 tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta cgcatgcgag ccaggttttc
    18181 cagccccata ttttcgccga gagtgatcag ctcagaaacc ggcgtattct ttaattcggt
    18241 aagattcata gtggtgtgag ttcttaaact tggggtaaat ctcgaactta atgttgtgaa
    18301 tggtatggca ggatcatcca tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct
    18361 ggtcacagga gagtacgcag aactgaaacg acaagacgga ttgagtgacg agccagaaat
    18421 ctgttcactt cgcatttaag ataaaacggg aagcgttgga aattacaaga ttcaaactta
    18481 ataaggtatg tttaatacga agtcaacact aagttagcat gactcacgcc gggcgtccag
    18541 tttttagcga cggggcaccc gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac
    18601 tctttcaact gacctttaga cagtgcgccc actttggttg ccgccacttc accgtttttg
    18661 aacagcagca gagtcgggat accacggatg ccatatttcg gcgcagtgcc agggttttga
    18721 tcgatgttca gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga
    18781 atcggggcga tcattttgca cggaccgcac cactctgccc agaaatcgac gaggatcgcc
    18841 ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg
    18901 ctcatatata actccacagg aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt
    18961 aaccaactaa aggttgactt tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg
    19021 atattctacc acactatgag caaaacacat ttaacagaac agaagttttc cgacttcgcc
    19081 ctgcatccga aggttgtaga agcccttgaa aaaaaagggt ttcataactg tacgcccatt
    19141 caggcactgg cccttccgct gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc
    19201 ggtaccggga aaacgatggc gtttcttacg tcaacgtttc attatcttct ctctcatcct
    19261 gcgattgccg atcgcaaggt gaatcaaccg cgtgccttaa ttatggcacc gacgcgtgaa
    19321 cttgccgtgc agatccatgc cgacgcagaa ccgctggcgg aagctactgg cctgaagctg
    19381 ggtctggctt acggtggtga tggctacgac aaacagctga aagtgctgga aagcggcgtt
    19441 gacattctga ttggcaccac gggtcgttta attgactacg ccaagcagaa ccacattaac
    19501 ctcggtgcca ttcaggtagt ggttctggat gaagccgatc gcatgtacga tctgggcttt
    19561 attaaagata tccgctggct gttccgccgt atgccgcctg caaaccagcg cctcaacatg
    19621 ctgttctccg ccacgctttc gtaccgggta cgtgaactgg cgttcgagca gatgaacaat
    19681 gccgaatata ttgaagtgga accggaacag aaaacgggcc accgtattaa agaagagctt
    19741 ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg
    19801 ccagacagag cgattatttt cgccaatacc aaacaccgtt gtgaagagat ctggggccac
    19861 ctggcggcag atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca gaaaaaacgt
    19921 ctgcgtattc ttgatgaatt tacccgtggc gatctggata ttctggttgc caccgacgtt
    19981 gccgcgcgtg gtttgcatat tccggcagtg acgcacgtct ttaactacga tttacccgat
    20041 gactgtgaag attacgttca ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac
    20101 tctatcagcc tggcgtgtga agagtatgca ttgaatttgc ctgctattga aacctatatt
    20161 ggtcactcaa ttccggtaag caaatacaat ccggacgcat tgatgaccga tctgccaaaa
    20221 ccgctgcgcc tcacgcgccc acgtacaggc aatggtccgc gtcgtactgg cgctccgcgt
    20281 aatcgtcgtc gttcaggtta aataaaatgt catactcaaa atcattcaaa ctgtatcaag
    20341 atggcaagag aatgaatccc gatgagcata ctgtagtaag tgattcaggt gaaagaatgc
    20401 agccaacaca gagacagatt gaaggatgaa gagtatgggt tccacctcgt cgctgtatgc
    20461 agccattgat ctcggttcga atagttttca tatgctggtt gtgcgcgagg tggctggaag
    20521 catccagacg ctgacgcgaa taaaacgcaa agtgcgtctg gctgctggcc tgaatagcga
    20581 aaatgccctg tctaatgaag caatggagcg cggttggcaa tgtctgcgcc tgtttgctga
    20641 acgtctgcaa gatatccctc cctcgcaaat tcgcgttgtc gctacggcga cattacgtct
    20701 tgccgtcaat gcgggtgatt ttattgccaa tgcgcaggaa atcctcggtt gtccggtaca
    20761 ggtgatcagc ggtgaagagg aagcacgtct gatttatcag ggcgttgctc acacaactgg
    20821 cggtgccgat cagcgcctgg tggtggatat aggcggtgcc agtactgaac tggtaaccgg
    20881 cacgggtgca caaaccacct cgttgttcag cctgtcgatg ggctgcgtca cctggctgga
    20941 acgctatttt gccgatcgta atctggggca ggaaaatttt gatgctgcag aaaaagcggc
    21001 acgcgaagtg ttacgtccgg ttgccgatga attacggtat cacggctgga aagtgtgcgt
    21061 tggtgcttcc ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg ggatggatga
    21121 acgcattacc ctggaaaagt tgcagcaatt gaaacagcga gccattcatt gcggtcggct
    21181 ggaggaactg gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc cgagtggtct
    21241 ggcgatcctg atcgccattt ttaccgaact gaatattcag tgtatgaccc tggcgggcgg
    21301 tgcgctgcgt gaaggcctgg tctacggcat gttgcatctg gccgtcgagc aggatattcg
    21361 cagccgtacg ctgcgtaata ttcagcgccg ctttatgatc gatattgatc aggcgcagcg
    21421 cgtagccaaa gttgcggcta acttcttcga tcaggtggaa aaggaatggc atcttgaagc
    21481 aataagccgc gatttgctca tcagcgcctg tcagcttcat gaaatcggcc tgagcgttga
    21541 ctttaaacaa gcgccgcaac acgctgctta tctggtgcgt aatctggatc ttcccggttt
    21601 tacccccgca cagaaaaaat tgctggcgac gctactgctc aaccagacta atccggtcga
    21661 tctctcatcg ctgcatcagc aaaatgccgt accgccgcgc gtcgcagaac aactctgccg
    21721 tttactccgc ctggccatca tttttgccag ccgtcgccgt gacgatctcg tgccagagat
    21781 gacattacag gctaaccatg aactgttgac cttgacgctt ccgcaaggtt ggctaaccca
    21841 acatccgctg ggtaaagaga ttattgctca ggaaagccag tggcagagct atgtccactg
    21901 gccgctggaa gtgcattaat gagtaagtgc cggatgcgat gctgacgcat cttttccggc
    21961 cttgattatt tccctcgttt tgccgccatc atcgctttca gattcgccag atggctttgc
    22021 cctttctgca tccgttcttc ggcgctgacc actttgcgct cctgttccca aatcagatca
    22081 tcctgcggca gctccagcaa aaagcggctc ggctccgggc gcaccagctc gccgtactga
    22141 cggcgttctt tacacagcgt aaaggtcaat tccttctggg cgcgggtaat gccgacatag
    22201 gccagccgcc gctcctcatc gatattatct tcatcgatgc tgctctggtg cggcaaaaac
    22261 ccttcttcca taccgaccat gtagacataa ggaaactcca gcccttttga cgcgtgcaga
    22321 gtcatcagtt gcacctgatc cagctcttct tcactctcac cacgctccat catgtcgcgc
    22381 aaagtaaagc gcgtcaccac ctgggtgagc gtcatcggct catccagttc gctgccttcc
    22441 agcatctccg tcatccagct aaacagttgg ttgacgttct tcatgcgcat ttcggcggct
    22501 ttcgggctgg gcgatgtttc gtacagccag gattcataat ccatgccgtg gatcagatcg
    22561 cgcaccgcgg caatcggctc ccgctccgcc agacgctgga tttctgccaa ccagtgagta
    22621 aagcgggtca atgcttcata accacgtccg ctaagcgtct gactcaggcc catatcaaag
    22681 ctggcggtaa acatgctttt attgcgcgtc atcgcccact cacccagctt tttcagcgtc
    22741 gccgggccaa tctctctctt cggcgtgtta acgatacgca gaaatgcgct gtcatcgtcc
    22801 gggttagtca gcacgcgcag ataagccagc aagtccttga tttcaggacg agagaaaaac
    22861 gacgtaccac cagaaatttt gtacgggatg cggttttgca tcaggaattt ttcaaacact
    22921 cgcgactgat ggttaccgcg ataaagaata gcgtaatctt tgtactgcgt tttattgacg
    22981 aagtgatgcg caatcagttc accagtgacg cgctcggctt catgttcttc gttattcgcg
    23041 cttaaaactt taagctctgt gccgtaaccc agttcggaga acagacgctt ctcaaagacg
    23101 tgcgggttat tggcgatcag gatgttcgcc gctttcaaaa tacgtccgga agagcggtag
    23161 ttctgctcaa gcttaatcac cttcagcgcc ggaaaatcct gactcagcag caccaggttt
    23221 tgtggacgtg cgccgcgcca ggagtagatc gactggtcgt catcgcccac cacggtaaag
    23281 cgcgcgcggc tgcccaccag cagtttcacc agttcatact ggctggtgtt ggtgtcctga
    23341 tactcatcca ccagcagata gcgaattttg ttctgccagc gctcgcggac ttcttcattg
    23401 cgttgcagca gcaacgtcgg caataaaatc agatcatcga agtcgagaac attgcacgct
    23461 ttcaggtgtg catcatacag cccataacaa tgggcaaaaa tacggtcccg ctcgccaatc
    23521 gcactggctg ccgcctggga cggtgttttg agatcgttct tccagttaga gatggtcgaa
    23581 atcaactgtt gcaggagaac tttgtcatct tcaatcagcc cttcggtcaa ctctttgagc
    23641 aacgccagct gatcggtatc gtcaaacagc gaaaagttcg ccttcatccc cagcgccgca
    23701 tactcacgct tgatgatatc cagccccagc gtgtggaagg ttgagatcat cagcccacgc
    23761 gcctctttgc gacccagcgt ctgtccgaca cgctctttca tctcgcgcgc cgccttattg
    23821 gtaaaggtca ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat cagatgggcg
    23881 attttattgg tgatcacacg agttttaccg gaacccgcgc ccgccagcac caggcagggg
    23941 ccggtaacga attcgacagc ttgttgttgg ccggggttta gacgcatagg tgtattgctc
    24001 aatcttcgaa cggggggagg attgtagcag aatcgcattt gaatgtgacc gcgtgtaagc
    24061 cgctaaccga ttggctgcgg atgtttaaat cagagaggga agaccgaaac cggagttcat
    24121 cgtgaatcca ttagacttag aaaatatcgg gtcgtaacgt tgtgttatta ctttttcaga
    24181 gtttactaag gagaggtgta ctcgtggcga ttaatttcag tcctaaggtt ggtgaaatac
    24241 tggattgcaa ctttggtaac tatcctgtta gccagaatgg acagttcagc accacgtatt
    24301 acgatggccg cattcctcct gaaatgataa aaaacaggtt agt
//
LOCUS       DAZOZD010000042        24048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOZD010000042 DAZOZD010000000
VERSION     DAZOZD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.042"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..91)
                     /locus_tag="ACHUPB_001352"
     CDS             complement(<1..91)
                     /locus_tag="ACHUPB_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0758212.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRH"
     gene            complement(149..382)
                     /locus_tag="ACHUPB_001353"
     CDS             complement(149..382)
                     /locus_tag="ACHUPB_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR0758213.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            771..1316
                     /locus_tag="ACHUPB_001354"
     CDS             771..1316
                     /locus_tag="ACHUPB_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0758214.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1291..3216
                     /locus_tag="ACHUPB_001355"
     CDS             1291..3216
                     /locus_tag="ACHUPB_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0758215.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3213..3419
                     /locus_tag="ACHUPB_001356"
     CDS             3213..3419
                     /locus_tag="ACHUPB_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0758216.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3416..5017
                     /locus_tag="ACHUPB_001357"
     CDS             3416..5017
                     /locus_tag="ACHUPB_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0758217.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4998..6317
                     /locus_tag="ACHUPB_001358"
     CDS             4998..6317
                     /locus_tag="ACHUPB_001358"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0758218.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6327..6659
                     /locus_tag="ACHUPB_001359"
     CDS             6327..6659
                     /locus_tag="ACHUPB_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0758219.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6715..7740
                     /locus_tag="ACHUPB_001360"
     CDS             6715..7740
                     /locus_tag="ACHUPB_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0758220.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7782..8180
                     /locus_tag="ACHUPB_001361"
     CDS             7782..8180
                     /locus_tag="ACHUPB_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0758221.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8192..8545
                     /locus_tag="ACHUPB_001362"
     CDS             8192..8545
                     /locus_tag="ACHUPB_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0758222.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8557..9135
                     /locus_tag="ACHUPB_001363"
     CDS             8557..9135
                     /locus_tag="ACHUPB_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0758223.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9132..9527
                     /locus_tag="ACHUPB_001364"
     CDS             9132..9527
                     /locus_tag="ACHUPB_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0758224.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9535..10275
                     /locus_tag="ACHUPB_001365"
     CDS             9535..10275
                     /locus_tag="ACHUPB_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0758225.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10291..10713
                     /locus_tag="ACHUPB_001366"
     CDS             10291..10713
                     /locus_tag="ACHUPB_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0758226.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10695..11129
                     /locus_tag="ACHUPB_001367"
     CDS             10695..11129
                     /locus_tag="ACHUPB_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0758227.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11122..13683
                     /locus_tag="ACHUPB_001368"
     CDS             11122..13683
                     /locus_tag="ACHUPB_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0758228.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13680..14009
                     /locus_tag="ACHUPB_001369"
     CDS             13680..14009
                     /locus_tag="ACHUPB_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0758229.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14009..14707
                     /locus_tag="ACHUPB_001370"
     CDS             14009..14707
                     /locus_tag="ACHUPB_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0758230.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14713..15456
                     /locus_tag="ACHUPB_001371"
     CDS             14713..15456
                     /locus_tag="ACHUPB_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0758231.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15453..16025
                     /locus_tag="ACHUPB_001372"
     CDS             15453..16025
                     /locus_tag="ACHUPB_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0758232.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16086..19583
                     /locus_tag="ACHUPB_001373"
     CDS             16086..19583
                     /locus_tag="ACHUPB_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0758233.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19654..20170
                     /locus_tag="ACHUPB_001374"
                     /pseudo
     CDS             19654..20170
                     /locus_tag="ACHUPB_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20235..23318
                     /locus_tag="ACHUPB_001375"
     CDS             20235..23318
                     /locus_tag="ACHUPB_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0758234.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23330..23899
                     /locus_tag="ACHUPB_001376"
     CDS             23330..23899
                     /locus_tag="ACHUPB_001376"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0758235.1"
                     /translation="MSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTDIAPP
                     DIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGEF
                     QKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKETLLL
                     EAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5682 a         6024 c         7352 g         4990 t
ORIGIN      
        1 tgtgacgttt tagttcagaa taaaaccagt gatagtttaa attatttcgc actttatcga
       61 atatttgttt aaaaatggca acctgagcca ttgtagtacc ttccatgtga tatgaggggg
      121 cgtagtctgc acgattatct aaattgcttc aatctggtct aacctgtttt ctgagcaatt
      181 cagtaatgtc actcttttct ttgtttgctt caggcgaaac tcttttttct gagcacagtc
      241 tccggcggca ggcttcaatg acccaggctg agaaattccc ggaccctttt tgaacaagag
      301 cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt gttgttctgc
      361 gggttctgtt cttcgttgac atgaggttgc cctgtattca gtgtcgctga tttgtattgt
      421 ctgaagttgt ttttacgtta agttgatgca gatcaattaa tacgatacct gcgtcataat
      481 tgattatttg acgtggtttg atggcgtaga tgcacgttgt gacatgtaga tgataattat
      541 tatcattttg cgggtccttt ccggcgatcc gacaggttac ggggcggaag gcgcgcgggt
      601 tttcgctatt tatgacaatt ttccggttta aggcgtttcc gttcttcttc gccgtaacct
      661 aatgttttta tttaaaacac cccctgaaaa gaaaggaaac gacaggtgct gaaaacgggc
      721 tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca atggaagtca
      781 acaaaaagca gctggctgac attttcggtg cgagtatccg taccattcag aactggcagg
      841 aacagggaat gcccgttctg cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg
      901 ccgccgttat aagatggtat gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc
      961 gggaagttga agaactgcgg caggccagcg agacagatct ccagccaggg actattgagt
     1021 acgaacgcca tcgacttacg cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag
     1081 actccgctga agtggtggaa accgcattct gtactttcgt gctgtcgcgg atcgcaggtg
     1141 aaattgccag tattctcgac gggatccccc tgtcggtgca gcggcgtttt ccggaactgg
     1201 aaaaccgaca tgttgatttc ctgaaacggg atatcatcaa agccatgaac aaagcagccg
     1261 cgctggatga actgataccg gggttgctga gtgaatatat cgaacagtca ggttaacagg
     1321 ctgcggcatt ttgtccgcgc cgggcttcgc tcactgttca ggccggagcc acagaccgcc
     1381 gttgaatggg cggatgctaa ttactatctc ccgaaagaat ccgcatacca ggaagggcgc
     1441 tgggaaacac tgccctttca gcgggccatc atgaatgcga tgggcagcga ctacatccgt
     1501 gaggtgaatg tggtgaagtc tgcccgtgtc ggttattcca aaatgctgct gggtgtttat
     1561 gcctacttta tagagcataa gcagcgcaac acacttatct ggttgccgac ggatggtgat
     1621 gccgagaact ttatgaaaac ccacgttgag ccgaccatcc gcgatattcc gtcgctgctg
     1681 gcgctggctc cgtggtatgg caaaaagcac cgggataaca cgctcactat gaagcgtttt
     1741 tccaatggtc gtggcttctg gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag
     1801 tcggtggatg tggcgggtta tgatgaactt gctgcctttg atgaggatat tgaacaggaa
     1861 ggctctccga cgttccttgg cgacaaacgt attgaaggct cggtctggcc aaagtccatc
     1921 cgtggctcca cgcccaaagt gagaggcacc tgccagattg agcgtgcagc cagtgaatcc
     1981 ccgcatttta tgcgttttca tgttgcctgc ccgcactgcg gggaggagca gtatcttaaa
     2041 tttggcgaca aagagacgcc gtttggcctc aaatggacgc cggatgaccc ctccagcgtg
     2101 ttttatctct gcgagcataa tgcctgcgtc atccgccagc aggagctgga ctttactgat
     2161 gcccgttata tctgcgaaaa gaccgggatc tggacccgtg atggcattct ctggttttcg
     2221 tcatccggtg aagagattga gccacctgac agtgtgacct ttcacatctg gacagcgtac
     2281 agcccgttca ccacctgggt gcagattgtc aaagactgga tgaaaacgaa aggggatacg
     2341 ggaaaacgta aaaccttcgt aaacaccacg ctcggtgaga cgtgggaggc gaaaattggc
     2401 gaacgtccgg atgctgaagt gatggcagag cggaaagagc attattcagc gcccgttcct
     2461 gaccgtgtgg cttacctgac cgccggtatc gactcccagc gggatcgcta cgaaatgcgc
     2521 gtatggggat gggggccggg tgaggaaagc tggctgattg atcggcagat tattatgggc
     2581 cgccacgacg atgaacagac gctgctgcgt gtggatgagg ccatcaataa aacctatacc
     2641 cgccggaatg gtgcagaaat gtcgatatcc cgtatctgct gggatattgg cgggattgac
     2701 ccgaccattg tgtatgaacg ctcgaaaaag catgggctgt tccgggtgat ccccattaaa
     2761 ggggcatccg tctacggtaa gccggtggcc agcatgcctc gtaagcgaaa caaaaacggg
     2821 gtttacctta ccgaaatcgg tacggatacc gcgaaagagc agatttataa ccgcttcaca
     2881 ctgacgccgg aaggggatga accgcttccc ggtgccgttc acttcccgaa taacccggat
     2941 atttttgatc tgaccgaagc gcagcagctg actgctgaag agcaggtcga aaaatgggtg
     3001 gatggcagga aaaaaatact gtgggacagc aaaaagcgac gcaatgaggc gctcgactgc
     3061 ttcgtttatg cgctggcggc gctgcgcatc agtatttccc gctggcagct ggatctcagt
     3121 gcactgctgg cgagcctgca ggaagaggat ggtgcagcaa ccaacaagaa aacactggca
     3181 gattacgccc gtgccttatc cggagaggat gaatgacgcg acaggaagaa cttgccgctg
     3241 cccgtgcggc actgcatgac ctgatgacag gtaaacgggt ggcaacggta cagaaagacg
     3301 gacggcgagt ggagtttacg gccacttccg tgtctgacct gaaaaaatac attgcggagc
     3361 tggaggtgca gaccggcatg acacagcgac gcaggggacc tgcaggattt tatgtatgaa
     3421 aacgcccacc attcccaccc ttctgggacc ggacggcatg acatcgctgc gtgaatatgc
     3481 cggttatcac ggcggtggca gcggatttgg tgggcagttg cgggcgtgga acccaccggg
     3541 tgaaagtgtg gatgcagccc tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga
     3601 tctggtacgc aataacggct atgctgccaa cgccatccag ctgcatcagg atcatatcgt
     3661 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga
     3721 agacgcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga
     3781 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt
     3841 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc
     3901 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa
     3961 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct
     4021 gggatattac gtcagcgagg acggctatcc tggctggatg ccgcagaaat ggacatggat
     4081 accccgtgag ttacccggcg ggcgcgcctc gttcattcac gtttttgaac ccgtggagga
     4141 cgggcagacc cgcggtgcaa atgtgtttta cagcgtaatg gagcagatga agatgctcga
     4201 cacgctgcag aacacgcagc tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat
     4261 tgaaagtgag ctggatacgc agtcagcgat ggattttatt ctgggcgcga acagtaagga
     4321 gcagcgggaa aggctgacgg gctggattgg tgaaattgcc gcgtattacg ccgcagcacc
     4381 ggtccgtctg ggaggcgcaa aagtgccgca cctgatgccg ggggactcac tgaacctgca
     4441 gacggctcag gacacggata acggctactc cgtgtttgag cagtcactgt tgcggtatat
     4501 cgctgccggg ctgggtgtct cgtatgagca actttcccgg aattacgccc agatgagcta
     4561 ctccacggca cgggccagtg cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt
     4621 cgtcgcatcc cgtcaggcga gccagatgtt tctgtgctgg ctggaagagg ccatcgttcg
     4681 ccgcgtggtg acgttacctt caaaagcgcg cttcagcttt caggaagccc gcagtgcctg
     4741 ggggaactgc gactggatag gctccggtcg tatggccatc gatggtctga aagaagttca
     4801 ggaagcggtg atgctgatag aagccggact gagcacctac gagaaagagt gcgcgaaacg
     4861 cggtgacgac tatcaggaaa tttttgccca gcaggtccgt gaaacgatgg agcgccgcgc
     4921 agctggtctt aaaccgcccg cctgggcggc tgcggcattt gaatccgggc tgcgacaatc
     4981 aacagaggag gagaagagtg acagcagagc tgcgtaatct cccgcatatt gccagcatgg
     5041 cttttaatga gccgctgatg cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg
     5101 caggccagct tgggatcagc tgcctgacgg atgcagtatc cggcgacagc ctgactgccg
     5161 gagaggcacc cgcggcgctg gcgttatccg gtgatgatga cggaccacga caggcccgca
     5221 gttatcaggt aatgaacggc atcgccgtgc tgccggtgtc cggcacgctg gtcagccgga
     5281 cgcgggcgct gcagccgtac tcggggatga ccggttacaa cggcattatc gcccgtctgc
     5341 aacaggctgc cagcgacccg atggtggacg gcattctgct cgatatggac acaccgggcg
     5401 gaatggtggc gggagcattt gactgcgctg acatcatcgc ccgtgtgcgt gacataaagc
     5461 cggtatgggc gctggccaat gacatgaact gcagcgcagg tcagctgctt gccagtgccg
     5521 cctcccggcg tctggtcacg cagaccgccc ggacaggctc catcggcgtc atgatggctc
     5581 acagtaatta cggcgctgca ctggagaaac agggcgtgga aatcacgctg atttacagcg
     5641 gcagccataa ggtggatggc aacccctaca gccatctacc ggatgatgtc cgggaaacat
     5701 tgcagtcccg gatggatgca acccgccgga tgtttgcaca gaaggtgtcg gcatataccg
     5761 gcctgtccgt gcaggctgtg ctggataccg aggctgcagt gtacagcggt caggaggcca
     5821 ttgatgccgg actggctgat gaacttgtta acagtaccga tgcgatcacc gtcatgcgtg
     5881 atgcactgga tgcacgtaaa tcccgtctct caggagggcg aatgaccaaa gagactcaat
     5941 caacaactgt ttcagccact gcttcgcagg ctgacgttac tggcgtggtg caagcgacgg
     6001 agggcgagaa cgccagcgcg gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg
     6061 cggcagaaaa cagtcgcatt atggggatcc tcaactgtga ggaggctcac ggacgcgaag
     6121 aacaggcacg cgtgctggcc gaaacccccg gtatgaccgt ggaaacggcc cgccgtattc
     6181 tggccgcagc accacagagt gcacagacgc gcagtgacac tgcgctggat cgtctgatgc
     6241 agggggcacc ggcaccgctg gctgcaggta acccggcatc tgatgccgtt aacgatttgc
     6301 tgaacacacc agtgtaaggg atgtttatga cgagcaaaga aacctttacc cattaccagc
     6361 cgctgggcaa tagtgacccg gctcataccg caaccgcgcc cggcggattg agtgcgaaag
     6421 cgcctgcaat gaccccgctg atgctggaca cctccacccg taagctggtt gcgtgggatg
     6481 gcaccaccga cggtgctgcc gttggcattc ttgcggttgc tgctgaccag accagcacca
     6541 cactgacgtt ctacaagtcc ggcacgttcc gttatgagga tgtgctctgg ccggaggctg
     6601 ccagcgacga gacgaaaaaa cggaccgtgt ttgccggaac ggcaatcagc atcgtttaac
     6661 tttacccttc atcactaaag gccgcctgtg tggctttttt tacgggattt ttttatgtcg
     6721 atgtacacaa ccgcccagct gctggcggca aatgagcaga aatttaagtt tgatccgctg
     6781 tttctgcgtc tctttttccg tgagagctat cccttcacta cggagaaagt ctatctctca
     6841 caaattccgg gactggtaaa catggcgctg tacgtttcgc cgattgtttc cggtgaggtt
     6901 atccgttccc gtggcggctc cacctctgaa tttacgccgg gatatgtcaa acccaagcat
     6961 gaggtgaatc cgcagatgac cctgcgtcgc ctgccggatg aagatccgca gaatctggcg
     7021 gacccggctt accgccgccg tcgcatcatc atgcagaaca tgcgtgacga agagctggcc
     7081 attgctcagg tcgaagagat gcaggcagtt tctgccgtgc tcaagggcaa atacaccatg
     7141 accggtgaag ccttcgatcc ggttgaggtg gatatgggcc gcagtgcggc gaacaacatc
     7201 acacagtccg gcgtcacgga gtggagcaag cgtgacaagt ccacgtatga cccgaccgac
     7261 gatatcgaag cctacgcgct gaacgccagc ggtgtggtga atatcatcgt gtttgacccg
     7321 aaaggctggg cgctgttccg ttccttcaaa gccgtcaagg agaagctgga tacccgtcgc
     7381 ggctctcatt ccgagctgga gacagcggta aaagacctgg gcaaagcggt gtcctataag
     7441 ggaatgtatg gcgatgtggc catcgtcgtg tattccggac agtacgtgga aaacggcgtc
     7501 aaaaagaact tcctgccgga caacacgatg gtgctgggga acactcaggc tcgcggtctg
     7561 cgcacctatg gctgcattca ggatgcggac gcacagcgcg aaggcattaa cgcttctgcc
     7621 cgttacccga aaaactgggt gaccaccggc gatccggcgc gtgagttcac catgattcag
     7681 tcagcaccgc tgatgctgct ggctgatcct gatgagttcg tgtccgttca actggcgtaa
     7741 tcatggccct tcggggccat tttctctctg tggaggagtt catgacgaaa gatgaactga
     7801 ttgcccgtct tcaggagctg ggtgagcaat tgaaccgcga tgtcagtttg acgggaacga
     7861 aagaagaact ggcgcttcgt gtggcagagc tggaagaaga gcttgatgac acggatgaaa
     7921 ctgccggtca ggacacccct ctcagccggg aaaatgtgct gaccgggcat gaaaatgagg
     7981 tggtatcagc gcagccggat accgtgattc aggatacggc tgaactggtc acggtcgtgg
     8041 cactggtgac gctgcatact gatgcacttc acgccacgcg ggataaacct gtggcatttg
     8101 tgctgccggg aacggcgttt cgtgtctctg ccggtgtggc agccgaaatg acagagcgcg
     8161 gcctggccag aatgcaataa cgggaggcgc tgtggctgat ttcgataacc tgttcgatgc
     8221 tgccattgcc cgcgccgatg aaacgatacg cgggtacatg ggaacgtcag ccaccatgac
     8281 atccggtgag cagtcaggtg cggtgatacg tggtgttttt gatgaccctg aaaatatcag
     8341 ctatgccgga cagggcgtgc gcgttgaagg ctccagcccg tccctgtttg tccggactga
     8401 tgaggtgcgg cagctgcggc gtggagacac gctgaccatc ggtgaggaaa atttctgggt
     8461 agatcgggtt tcgccggatg atggcggaag ttgtcatctc tggcttggac ggggcgtacc
     8521 gcctgccgtt aaccgtcgcc gctgaaaggg ggatgtatgg ccataaaagg tcttgagcag
     8581 gccgttgaaa acctcagccg tatcagcaaa acggcggtgc ccggtgccgc cgcaatggcc
     8641 attaaccgcg ttgcttcatc cgcgatatcg cagtctgcgt cacaggttgc ccgtgagaca
     8701 aaggtacgcc ggaaactggt aaaggaaagg gccaggctga aaagggccac ggtcaaaaat
     8761 ccgcaggcca gaatcaaagt taaccggggg gatttgcccg taatcaagct gggtaatgcg
     8821 cgggttgtcc tgtcccgccg caggcgtcat aaaaaggggc agcgttcatc cctgaaaggt
     8881 ggcggcagcg tgcttgtggt gggtaaccgt cgtattcccg gcgcgtttat tcagcaactg
     8941 aaaaacggcc ggtggcatgt tatgcagcgt gtggccggga aaaaccgtta ccccattgat
     9001 gtggtgaaaa tcccgatggc ggtgccgctg accacggcgt ttaaacagaa tattgaacgg
     9061 atacggcgtg aacgtcttcc gaaagagctg ggctatgcgc tgcagcatca actgagaatg
     9121 gtaataaagc gatgaaacat actgaactcc gtgcagccgt actggatgca ctggagaagc
     9181 atgacaccgg ggcgacgctt tttgatggtc gccccgctgt ttttgatgag gaagattttc
     9241 cggcaattgc cgtttatctc accggcgctg aatacacggg cgaagagctg gacagcgata
     9301 cctggcaggc ggagctgcat attgaagttt tcctgcctgc tcaggtgccg gattcagagc
     9361 tggattcgtg gatggagtcc cggatttatc cggtgatgag cgatatcccg gcactgtcag
     9421 atttgatcac cagtatggtg gccagcggct atgactaccg gcgcgacgat gatgcgggcc
     9481 tgtggagttc agccgatctg acttatgtca ttatctatga aatgtgagga cgctatgcct
     9541 gtaccaaatc ctacaatgcc ggtgaaaggt gccgggacca ccctgtgggt ttataagggg
     9601 agcggtgacc cttatgcgaa cccgctttca gacgttgact ggtcgcgtct ggcaaaagtt
     9661 aaagacctga cgcccggcga actgaccgct gagtcctatg acgacagcta tctcgatgat
     9721 gaagatgcag actggactgc gaccgggcag gggcagaaat ctgccggaga taccagcttc
     9781 acgctggcgt ggatgcccgg agagcagggg cagcaggcgc tgctggcgtg gtttaatgaa
     9841 ggtgataccc gtgcctataa aatccgcttc ccgaacggca cggtcgatgt gttccgtggc
     9901 tgggtcagca gtatcggtaa ggcggtgacg gcgaaggagg tgattacccg cacggtgaag
     9961 gtcaccaatg tgggacgtcc gtcgatggca gaagatcgca gcacggtgac ggcgacgacc
    10021 ggcatgacgg tgacacccgc cagtgcttcc gtagtgaaag ggcagagcac cacgctgacc
    10081 gtggcattcc agccggatgg cgcaaccgac aagagcttcc gtgcggtgtc tgcggataaa
    10141 acaaaagcca ccgtgtcggt cagtggtatg accatcaccg tgaaaggtgt tgctgcaggc
    10201 aaggtcaaca ttccggtcgt atctggtaat ggtgagtttg ctgcggttgc agaaatcaac
    10261 gtcaccgcca gttaatccgg agagtcagcg atgttcctga aaaccgaatc atttgaacat
    10321 aacggtgtga ccgtcacgct ttctgaactg tcagccctgc agcgtattga gcatctcgac
    10381 ctgatgaaac ggcaggcaga acaggcggag tcagacagca accggaagtt tactgtggaa
    10441 gacgccatca gaaccggcgc ttttgtggtt gcgatgtccc tgtggcataa ccatccgaag
    10501 aagacgcaga tgccgtccat gaatgaagcc gttaaacaga ttgagcagga agtgcttacc
    10561 acctggccca cagaggcaat ttctcatgct gaaaacgtgg tgtaccggct gtccggtatg
    10621 tatgagtttg tggtgaatga tgctcctgaa caggcagagg acgccgggcc tgcagagcct
    10681 gtttctgcgg gaaagtgttc gacggtgagc tgagttttgc cctgaaactg gcgcgtgaga
    10741 tggggcgacc cgactggcgc gccatgcttg ccgggatgtc atccacggag tatgccgact
    10801 ggcaccgctt ttacagtacc cattattttc atgatgttct gctggatatg cacttttccg
    10861 ggctgacgta caccgtgctc agcctgtttt tcagcgatcc ggagatgcat ccgctggatt
    10921 tcagtctgct gaaccggcgc gaggctgacg aagagcctga agatgatgtg ctgatgcaga
    10981 aagcggcagg gcttgccgga ggtgtccgct ttggcccgga cgggaatgaa gttatccccg
    11041 cttccccgga tgtggcggac atgacggagg atgacgtaat gctgatgaca gtatcagaag
    11101 ggatcgcagg aggagtccgg tatggctgaa ccggtaggcg atctggtcgt tgatttgagt
    11161 ctggatgcgg ccagatttga cgagcagatg gccagagtca ggcgtcattt ttccggtacg
    11221 gaaagtgatg cgaaaaaaac agcggcagtc gttgaacagt cgctgagccg acaggcgctg
    11281 gctgcacaga aagcggggat ttccgtcggg cagtataaag ccgccatgcg tatgctgcct
    11341 gcacagttca ccgacgtggc cacgcagctt gcaggggggc agaatccctg gctgatcctg
    11401 ctgcaacagg gtggtcaggt taaggactcc ttcggcggga tgatccccat gttcaggggg
    11461 cttgccggcg cgatcaccct gccgatggtg ggggccacct cgctggcggt ggcgaccggt
    11521 gcgctggcgt atgcctggta tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg
    11581 gtcctttccg gtaatcaggc gggactgacg gcagatcgta tgctggtcct gtccagagcc
    11641 gggcaggcgg cagggctgac gtttaaccag accagcgagt cactcagcgc actggttaag
    11701 gcgggggtaa gcggtgaggc tcagattgcg tccatcagcc agagtgtggc gcgtttctcc
    11761 tatgcatccg gcgtggaggt ggacaaggtc gctgaagcct tcgggaagct gaccacagac
    11821 ccgacgtcgg ggctgacggc gatggcacgc cagttccata acgtgacggc ggagcagatt
    11881 gcgtatgttg ctcagttgca gcgttccggc gatgaagccg gggcattgca ggcggcgaac
    11941 gaggccgcaa cgaaagggtt tgatgaccag acccgccgcc tgaaagagaa catgggcacg
    12001 ctggagacct gggcagacag gactgcacgt gcattcaaat ccatgtggga tgcggtgctg
    12061 gatattggtc gtcctgatac cgcgcaggag atgctgatta aggcagaggc tgcgtttaag
    12121 aaagcagacg acatctggaa tctgcgcaag gatgattatt tcgttaacga tgaagcacgg
    12181 gcgcgttact gggatgatcg tgaaaaaaaa cgcctggagc gggatgcagc ccaaaagagg
    12241 gtagatcaac agcgtcaaca ggacaaaaat gcgcagcagc agagcgatac cgaagcgtca
    12301 cggctgaaat ataccgaaga ggcgcagaag gcttacgaac ggctgcagac gccgctggag
    12361 aaatataccg cccgtcagga agaactgaac aaggcactga aagacggaaa aatcctgcag
    12421 gcagattaca acacgctgat ggcggcggcg aaaaaggatt atgaagcgac gctgaaaaag
    12481 ccgaaacagt ccggcgtgaa ggtgtatgcg ggcgatcgtc aggaagacag tgctcatgcg
    12541 gccctgctga cgcttcaggc agaactccgg acgctggaga agcatgccgg agcgaatgag
    12601 aaaatcagcc agcagcgccg ggatttgtgg aaggcagaaa gtcagttcgc ggtactggag
    12661 gaggcggcac aacgtcgcca gctgtccgca caggagaaat ccctgctggc gcataaagac
    12721 gagacgctgg agtacaaacg ccagctggct gcacttggtg acaaggttac gtatcaggag
    12781 cacctgaatg cgctggcgca gcaggcggat aaattcgcac agcagcaacg ggcaaaacgg
    12841 gcagccattg atgcgaaaaa ccgggggctg actgaccggc aggcagcgcg ggaagccacg
    12901 gaacagcgcc tgaaggaaca gtatggcgat aatcctctgg cgctgaataa cgtcatgtca
    12961 gagcagaaaa agacctgggc ggctgaagac cagcttcgcg ggagctggat ggcaggcctg
    13021 aagtccggct ggagtgagtg ggaagagagc gccacggaca gtatgtcaca ggttaaaagt
    13081 gcagccacgc agacctttga tggtattgca cagaatatgg cggcgatgct gaccggcagt
    13141 gaacagaact ggcgcagctt cacccgctcc gtgctgtcca tgatgacaga aattctgctt
    13201 aagcaggcaa tggtggggat tgtcgggagt atcggcagcg ccattggcgg ggctgttggt
    13261 ggcggcgcat ccgcgtcagg tggtacagcc attcaggccg ctgcggtgaa attccatttt
    13321 gcaaccgggg gatttacggg aaccggcggc aaatatgagc cagcggggat tgttcaccgt
    13381 ggtgaatttg tcttcacgaa ggaggcaacc agccggattg gcgtggggaa tctctaccgg
    13441 ctgatgcgcg gctatgcgga aggtggttat gtgggcggtg ccggaagtcc ggcgcagatg
    13501 cggcgggctg aaggcattaa ttttaatcag aacaatcacg tggtgattca gaacgacggt
    13561 acgaatggtc tgccaggtcc acagatgatg aaggccgtgt atgacatggc ccgcaagggt
    13621 gcccgtgatg aaattcagac acagatgcgt gatggtggcc tgttctccgg aggtggacga
    13681 tgaaaacctt ccgctggaaa gtgaaacccg gtatggatgt ggcttcggcc ccttctgtaa
    13741 gaaaggtgcg ctttggtgat ggctattccc agcgagcgcc tgccgggctg aatgccaacc
    13801 tgaaaacgta cagcgtgacg ctttctgtcc cccgtgagga ggccatggta ctggagtcgt
    13861 ttctggaaga gcacgggggc tggaaagcct ttctgtggac gccgccttat gagtggcggc
    13921 agataaaggt gacctgcgca aaatggtcgt cgcgggtcag tatgctgcgt gttgagttca
    13981 gcgcagagtt tgaacaggtg gtgaactgat gcaggatatc cggcaggaaa cactgaatga
    14041 atgcacccgt gcggagcagt cggccagcgt ggtgctctgg gaaatcgatc tgacagaggt
    14101 cggtggagaa cgttattttt tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg
    14161 gcaggggcga cagtatcagc cgtatcccat tcaggggagt ggttttgaac tgaatggcaa
    14221 aggcaccagt acgcgcccca cgctgatggt ttctaacctg tacggtatgg tcaccgggat
    14281 ggcggaagat ctacagagtc tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg
    14341 ttttctggat gcggtgaact tcgtcaacgg aaacagtgac gccgatccgg agcaggaggt
    14401 gatcagccgc tggcgcattg agcagtgcag cgaactgagc gcggtgagtg cctcttttgt
    14461 actgtccacg ccgacggaaa cagatggcgc tgtttttccg ggacgtatca tgctggccaa
    14521 cacctgcacc tggacctatc gcggcgatga gtgcggttat cacggtccgg cggtcgcgga
    14581 tgaatatgac cagccaacgt ccgacatcac gaaggataaa tgcagcaaat gcctgagtgg
    14641 ctgtaagttt cgcaataacg tcggcaactt tggcggcttc ctttccatta acaaactttc
    14701 gcagtaaatc ccatgacaga gacagaatca gcgattctgg cgcacgcccg gcgatgtgct
    14761 ccagcggagt cgtgcggctt cgtggtaaga acgccggagg gggaaagata tttcccctgc
    14821 gtgaatatct ccggtgagcc ggaggcgtat ttccgtatgt cgccggaaga ctggctgcag
    14881 gcagagatgc agggtgagat tgtggcactg gtccacagcc accccggtgg tctgccctgg
    14941 ctgagtgagg ccgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc
    15001 cggggggcga ttcacaagtt ccgctgtgtg ccacatctta ccgggcggcg ctttgagcac
    15061 ggggtgacgg actgttacac gctgttccgg gatgcttacc atctggcggg gattgagatg
    15121 ccggattttc atcgtgagga tgactggtgg cgtcacggtc agaatctcta tctggataat
    15181 ctggaggcca cagggctgta tcaggtgccg ttgtcagcgg cgcagccggg cgatgtgctg
    15241 ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgtgg tgacggcgag
    15301 ctgctgcacc atattcctga acaactgagc aaacgagaga ggtataccga caaatggcag
    15361 cgacgcacac actccctctg gcgtcaccgg gcatggcacg catctgcctt tacggggatt
    15421 tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac gggtgccgaa gccatccggg
    15481 cgatggccac acagctccct gcgtttcgtc agaagctgag cgacggctgg tatcaggtgc
    15541 gtattgccgg gcaggatgtt agcacgtccg gattaacggc acagttacat gaggctctgc
    15601 ctgacggcgc tgtgattcat attgttccca gagtcgccgg ggccaagtca ggtggtgtat
    15661 tccagattgt cctgggagca gccgccattg ccggatcatt ctttaccgcc ggagccaccc
    15721 ttgcagcatg gggggctgcc attggtgccg gtggtatgac cggcattctg ttttctctcg
    15781 gtgccagtat ggtgctcggc ggtgtggctc agatgctggc accgaaagcc agaactcccc
    15841 gcacacagac aacggataac ggtaagcaga acacctattt ctcctcactg gataacatgg
    15901 ttgcccaggg caatgttatg cctgttctgt atggtgaaat gcgcgtgggg tcacgtgtgg
    15961 tatctcagga gatcagcacg gcagacgaag gggacggtgg tcaggttgtg gtgattggtc
    16021 gctgatgcaa aatgttttat gtgaaatcgc ctccgggcgg ttttatcgtt tatggagcat
    16081 gacgaatggg taaaggcagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga
    16141 agtccacgca gctgctgagt gtgatcgatg ccatcagcga agggccggtt gaaggtccgg
    16201 tggatggatt aaaaagcgtg ctgctgaaca gtacgccggt gctggacagt gaggggaata
    16261 ccaacatctc cggcgtcacg gtggtgttcc gggcaggtga gcaggagcag tcaccgccgg
    16321 agggatttga atcctccggc tccgagacgg tgctgggtac ggaagtgaaa tatgacacgc
    16381 cgatcacccg gaccatcacg tcggcaaaca ttgaccgact gcgttttacc ttcggtgtac
    16441 aggcactggt ggaaaccacc tcaaagggag acaggaatcc gtctgaagtt cgcctgctgg
    16501 ttcagataca gcgtaacggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa
    16561 ccacctcgca gtatctggcc tcggtggtgg tggataacct gccgccgcgc ccgtttaata
    16621 tccggatgcg cagaatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct
    16681 ggtcgtcata caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg
    16741 gcgtacaggt ggattcggag cagttcggca gccagcaggt gagccgtaat tatcatcttc
    16801 gcgggcgcat tctgcaggtg ccgtcgaact ataatccgca gacgcggcaa tacagcggta
    16861 tctgggacgg aacgttaaaa ccggcataca gcaacaacat ggcctggtgt ctgtgggata
    16921 tgctgaccca tccgcgctat ggcatgggga aacgtcttgg tgcggcagat gtggataaat
    16981 gggcgctgta tgtcatcggc cagtactgtg accagtcggt gccggacggc tttggcagca
    17041 cggagccgcg catcacctgt aatgcctggc tgaccacaca gcgcaaggcg tgggatgtgc
    17101 tcagtgattt ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt
    17161 tcgtgcagga ccgacagtcg gataaggtgt gggcctataa ccgcagtaat gtggtgatgc
    17221 cggatgatgg cgcgccgttc cgctacagtt tcagcgccct gaaagaccgc cataatgccg
    17281 ttgaggtgaa ctggattgac ccggataacg gctgggagac ggcgacagag cttgtggagg
    17341 atacgcaggc cattgtccgt tacggtcgta acgtcacgaa gatggatgcc tttggctgta
    17401 ccagccgggg gcaggcacac cgcgccgggc tgtggctgat taaaacggaa ctgctggaga
    17461 cgcagaccgt ggatttcagc gtgggtgctg aagggcttcg tcatgtaccg ggcgatgtca
    17521 ttgaaatctg cgatgatgac tatgccggta tccgcaccgg cgggcgcgtg ctggcggtga
    17581 acagccagac ccggacgctg acgctcgacc gtgaaatcac gctgccatcc tccggcacca
    17641 cgctgataag cctggttgac gggcagggga atccggtcag cgtggaggtc cagtccgtca
    17701 ccgacggcgt gaaggtaaaa gtgagccgtg ttcctgacgg tgttgctgaa tacagcgtgt
    17761 gggggctgaa gctgccgacg ctgcaccagc gattgttccg ctgcgtgagt atccgtgaga
    17821 acgacgacgg cacgtatgcc atcaccgccg tgcagcatgt gccggaaaaa gaagccatcg
    17881 tggataacgg ggcgcacttt gacggcgacc agagcggcac ggtgaatggt gtcacgccgc
    17941 cagcggtgca gcacctgact gccgaagtca ccgcagacag cggggaatat caggtgctgg
    18001 cgcgctggga cacgccgaag gtggtgaagg gcgtgagctt cctgctccgt ctgaccgtaa
    18061 cagcggatga cggcagtgag cggctggtca gcacggcccg gacgacggaa accacttacc
    18121 gcttcacaca actggcgctg gggaactaca ggctgacagt ccgggcagta aatgcgtggg
    18181 ggcagcaggg cgatccggcg tcggtatcgt tccggattgc cgcaccggca gcgctgtcgc
    18241 ggattgagct gacgccgggc tattttcaga taaccgccac gccgcatctt gcggtttatg
    18301 atccgacggt acagtttgag ttctggttct cggaaaagcg gattgcggat atcaggcagg
    18361 ttgaaaccac agcacgctat cttggcacgg cgctgtactg gatagccgcc agtatcaata
    18421 tcaaaccggg ccatgattat tacttttata tccgcagtgt gaacaccgtt ggcaaatcgg
    18481 cattcgtgga ggctgttggt cagccgagtg atgatgcatc cggttatctg gattttttca
    18541 aaggcgagat agggaaaacc catctggctc aggagctgtg gacgcagatt gataacggtc
    18601 agcttgcgcc tgacctggct gaaatcagga cgtccattac ggatgtcagc aatgaaatca
    18661 cgcagaccgt caataagaaa ctggaagacc agagtgcggc aattcagcag atacagaagg
    18721 ttcaggttga tacaaataat aacctgaaca gcatgtgggc tgtgaagctg cagcagatgc
    18781 aggacggacg cctttatatc gcgggtattg gtgccggtat tgagaacacc cctgacggca
    18841 tgcagagtca ggtgctgctg gcggcggaca ggattgcgat ggttaatcct gcgaatggca
    18901 acacaaaacc gatgtttgtt ggtcagggcg atcagatatt catgaacgac gtgttcctga
    18961 aacgcctgac ggcccccacc attaccagcg gtggaaatcc atcggcattt tccctgactc
    19021 cggacggaaa gctgaccgct aaaaatgcgg atatcagtgg cagtgtgaat gcgaacgccg
    19081 ggacgctcaa caatgtcacg gtaaatgaaa actgtacgat taagggcatg ctggaggcga
    19141 ctcaggtcag aggtgacttc gttaaagctg tatccaaatc atttccgaaa caggctggta
    19201 cgtggggtaa cacggaaaca ccaaacggga cggttacagt caccataagc gatgatcata
    19261 actttgaccg ccagattatt attccgccca ttatttttaa cggtatagcg tatgacgatc
    19321 cggggagcgg aaataaccca ggaggcacgc gatacacggg ttatggtttt gaagttcgca
    19381 aaaacggcgt attaatcgca tccagagaaa ctaaaggtgc cattcccggt agttacagtg
    19441 cagttattga tatgcctagt ggtggtggta gcgtcactct ggagtttaag attttccaga
    19501 aaggcaatca gggggcaggc aatatcaccg actgtacggt gattgtgacc aaaaaagcgg
    19561 cttccggcat cagtattcgt tgaaatattt ataaccccaa taaatggcgt caggaatgac
    19621 gcctttttta ttgcagaaaa gcgagaggta attatgcgta aagtttgtgc agccattttg
    19681 tccgcagcca tttgcctggc cgtatccggt gcgcctgcat gggcgtctga acatcagtcc
    19741 acgctgagcg cggggtatct tcatgcccgg accaacgttc ccggcagtga tgatctgaac
    19801 gggattaacg tgaaataccg ttatgagttt acggatacgc tggggctggt gacgtcattc
    19861 agctatgcag gagacaagaa tcgccagctg acccgttaca gcgatacccg ctggcatgaa
    19921 gattccgtgc gtaaccgctg gttcagcgtg atggcggggc cgtctgtgcg cgtgaatgaa
    19981 tggttcagcg cgtatgcgat ggcgggtgtg gcttacagcc gtgtgtcgac tttttccggg
    20041 gattatatcc gcgtaactga caataagggg aaaacgcacg atgtgctgac cggaagtgat
    20101 gacggtcgcc acagcaacac gtctctggcg cactgacggt ttcatcgtgg gtgtcggtta
    20161 taaattctga ttagccaggt aacacagtgt tatgacagcc cgccggttca ggcgggcttt
    20221 tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagacggc acaggaaaac
    20281 cggtacagaa ctgcacaatc cagctgaaag caaaacgtaa cagcaccacg gtggtggtga
    20341 acacgctggc ctcagaaaat ccggatgaag ccgggcgtta cagcatggac gttgagtacg
    20401 gtcagtacag cgttattctg ttggtggaag gattcccgcc gtcacatgcc gggaccatca
    20461 ccgtgtatga agattcccga cccggtacgc tgaatgattt tctcggtgcc atgacggagg
    20521 atgatgcccg tccggaggca ctgcgccgtt ttgagctgat ggtggaagag gtggcgcgta
    20581 acgcgtccgc ggtggcacag aacacggcag ccgcgaagaa atcagccagc gatgccagca
    20641 catcagcccg tgaggcggca acccatgcga ctgatgctgc agattcagca cgtgcagcca
    20701 gcacgtcagc cggacaggcc gcttcgtcgg ctcagtcagc gtcttccagc gcaggaacgg
    20761 catcaacaaa ggccactgaa gcctcaaaaa gtgctgccgc tgcagagtcc tcaaaaagcg
    20821 cggcggctac cagtgccggt gcggcgaaaa cgttagaaac gaatgcggca gcgtcacaac
    20881 aatcagcagc cacttctgca tccaccgcga ccacgaaagc gtcagaagct gccacctcag
    20941 cccgggatgc gtcggcttca aaagaggcgg caaaatcatc agaaacgaac gcatcatcaa
    21001 gcgccagtag cgcagcttcc tcggcgacgg cggctgcaaa ttctgcgaag gcggccaaaa
    21061 cgtccgagac gaacgccagg tcttctgaaa cggcagcggg acagagtgcc tcagctgcgg
    21121 caggctcaaa aacagcggct gcgtcgtctg ccagcgccgc gtcaacaagt gccgggcagg
    21181 cctcagccag tgccaccgcc gccggaaaat cggcagaaag cgccgcatca tccgcttcaa
    21241 cagccacaac gaaggctggc gaagccactg agcaggccac ggcagcagcg aggtcagctt
    21301 ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga aacccgtgca gaatcctcaa
    21361 aaacggctgc cgcatcgtcc gccagttcgg cggcgtcatc ggcatcatcg gcgtctgctt
    21421 caaaagatga ggcgaccaga caggcgtcag cagcgaaggg cagcgccacg acggcatcca
    21481 cgaaggcgac agaggctgct ggtagtgcga cggcagcagc tcagagcaaa agtacggcgg
    21541 aatctgcagc aacgcgcgct gagacagcgg caaaacgggc agaggatatt gcatccgccg
    21601 tggcgcttga ggatgcgagc acgacgaaaa aggggatagt acagctcagt agtgcgacta
    21661 acagcacttc cgagtcactg gcggcaacac caaaagccgt taaggccgcg tatgacctgg
    21721 ctaacgggaa atacaccgca caggatgcaa cgacagcaca gaaagggata atccagctaa
    21781 gcagcgcgac caacagcacg tctgaaacgc tggcggcaac gccaaaggca gtaaaagcag
    21841 ccaatgacaa tgctgagaaa cgtctgcaga aagatcagaa cggtgcggat atccctggca
    21901 aagacacctt tacgaaaaat attggtgcct gccgtgcctt cggtgggtca gtaagcacaa
    21961 caacaggaaa ctggacgact gcacagttta tcgagtggct ggattctcag ggagcattta
    22021 accatccata ctggatgtgc aagggttcct ggtcttatgg caataataaa atcattactg
    22081 atactggctg cggtaatatt catctcgccg gagctgtcat tgaagtaatg gggataaagt
    22141 cagcgatgac gatccgcatt accactccga ccacatccag cggtggcgga acaaccaacg
    22201 cccagtttac ctatattaat cacggaacag attattcacc tggctggcga agggactata
    22261 actccagaaa taagccaacg gcatcagaga tcggggcgct accgtcaggt ggaacagcag
    22321 tatcatcagt taatctggct tcaaaaggtc gggtagccgc gctgacagac aatacgcagg
    22381 gggcagcagg tcttgagtta tacgaggtgt ataacaacgg atatccaaca gcgtatggaa
    22441 atatcattca cctgaaaggg atgacagccg ttggcgaagg tgagttactc atcggctgga
    22501 gtggtacaag cggtgctcat gctccggcat ttattcgttc acgacgggat acgaccgacg
    22561 caaactggtc gccgtgggcg cagctttaca cctcggctca tcctcctgca gagttttatc
    22621 cagtcggtgc accaatcccg tggccatcag ataccgttcc gtctggttat gccctgatgc
    22681 aggggcagac ttttgacaaa tctgcttacc cgaaacttgc agccgcttat ccgtcaggcg
    22741 tgatccctga tatgcgtggc tggacgatta agggcaagcc cgccagtggt cgtgccgtat
    22801 tgtctcagga acaggatggc attaaatcgc acacccacag cgccagcgca tccagtacgg
    22861 atttggggac gaaaaccaca tcgtcgtttg attacggcac taaatccacg aataacaccg
    22921 gggcgcatac gcacagtctg agcggctcta caagttcagc tggggcccac caacatagtc
    22981 agactgggcc cagaacgaat agcgggagtc aacctactgg aatgtttccg gcagggagta
    23041 ctcaagttag cgggactaat caggttggta tcagtggctc attaaccagt ggcacctccc
    23101 aatgggtagg taaatcatct tcagaaggaa accatacaca ctcactgtcc ggcacagcag
    23161 catctgcggg tgcacacgcg catactgtcg gtattggtgc tcatacgcac tctgttgcga
    23221 ttggttcaca tggacacacc atcaccgtta acgctgctgg taacgcggaa aacaccgtca
    23281 aaaacatcgc atttaactat attgtgaggc tcgcataatg gcattcagaa tgagtgaaca
    23341 agcacggacc ataaaaattt ataacctgct ggccggaact aatgaattta ttggtgaagg
    23401 tgacgcatat attccgcctc atacaggtct gcctgcaaac agtaccgata ttgcaccgcc
    23461 agatattccg gctggtttcg tggctgtttt caacagtgat gagtcatcgt ggcatctcgt
    23521 tgaagatcat cggggtaaaa cggtttatga cgtggcttcc ggcgacgcgt tatttatttc
    23581 tgaactcggc tcattaccgg aaaatgtcac ctggttatcc ccggaagggg agtttcagaa
    23641 gtggaacggc acagcctggg tgaaggatac ggaagcagaa aaactgttcc ggatccggga
    23701 ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt gagcatatag cgccgcttca
    23761 ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc ttgttgctgg aagcttggaa
    23821 aaagtatcgg gtgttgctga accgtgttga tacatcaact gcaccggata ttgaatggcc
    23881 agtagcacct atagggtaaa ttcgtaatga ttacctaaat acgttattct tttgttaaaa
    23941 aagtgatttt gctataggta gggagtatgt tcttggatta agaataaaaa atcgccatga
    24001 aagagcttca tggcgagtac ataaacgtac aaaatgtgat gaaagtag
//
LOCUS       DAZOZD010000043        23450 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOZD010000043 DAZOZD010000000
VERSION     DAZOZD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23450
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.043"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUPB_001377"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUPB_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR0758236.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUPB_001378"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUPB_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR0758237.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUPB_001379"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUPB_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0758238.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUPB_001380"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUPB_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0758239.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUPB_001381"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUPB_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0758240.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUPB_001382"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUPB_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR0758241.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUPB_001383"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUPB_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0758242.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUPB_001384"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUPB_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0758243.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUPB_001385"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUPB_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUPB_001386"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUPB_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0758244.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUPB_001387"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUPB_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR0758245.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUPB_001388"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUPB_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0758246.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUPB_001389"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUPB_001389"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0758247.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHUPB_001390"
     CDS             complement(14171..14743)
                     /locus_tag="ACHUPB_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0758248.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUPB_001391"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUPB_001391"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0758249.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUPB_001392"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUPB_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0758250.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHUPB_001393"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHUPB_001393"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0758251.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHUPB_001394"
     CDS             17912..18760
                     /locus_tag="ACHUPB_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0758252.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUPB_001395"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUPB_001395"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0758253.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUPB_001396"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUPB_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0758254.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHUPB_001397"
     CDS             complement(21672..21872)
                     /locus_tag="ACHUPB_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0758255.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUPB_001398"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUPB_001398"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0758256.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHUPB_001399"
     CDS             complement(23031..23387)
                     /locus_tag="ACHUPB_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0758257.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5870 a         6229 c         6274 g         5077 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctagccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagttatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacatt actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgctattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcgggtcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc
//
LOCUS       DAZOZD010000044        22804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOZD010000044 DAZOZD010000000
VERSION     DAZOZD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.044"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            76..705
                     /locus_tag="ACHUPB_001400"
     CDS             76..705
                     /locus_tag="ACHUPB_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0758258.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            732..2393
                     /locus_tag="ACHUPB_001401"
     CDS             732..2393
                     /locus_tag="ACHUPB_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0758259.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            2632..2691
                     /locus_tag="ACHUPB_001402"
     CDS             2632..2691
                     /locus_tag="ACHUPB_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0758260.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3008..3067
                     /gene="ibsE"
                     /locus_tag="ACHUPB_001403"
     CDS             3008..3067
                     /gene="ibsE"
                     /locus_tag="ACHUPB_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR0758261.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3187..4620)
                     /gene="hldE"
                     /locus_tag="ACHUPB_001404"
     CDS             complement(3187..4620)
                     /gene="hldE"
                     /locus_tag="ACHUPB_001404"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0758262.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4668..7508)
                     /gene="glnE"
                     /locus_tag="ACHUPB_001405"
     CDS             complement(4668..7508)
                     /gene="glnE"
                     /locus_tag="ACHUPB_001405"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0758263.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7531..8832)
                     /gene="ygiF"
                     /locus_tag="ACHUPB_001406"
     CDS             complement(7531..8832)
                     /gene="ygiF"
                     /locus_tag="ACHUPB_001406"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0758264.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9074..9694
                     /locus_tag="ACHUPB_001407"
     CDS             9074..9694
                     /locus_tag="ACHUPB_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0758265.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9758..10996
                     /gene="cca"
                     /locus_tag="ACHUPB_001408"
     CDS             9758..10996
                     /gene="cca"
                     /locus_tag="ACHUPB_001408"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0758266.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11159..11980)
                     /gene="bacA"
                     /locus_tag="ACHUPB_001409"
     CDS             complement(11159..11980)
                     /gene="bacA"
                     /locus_tag="ACHUPB_001409"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0758267.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12071..12439)
                     /gene="folB"
                     /locus_tag="ACHUPB_001410"
     CDS             complement(12071..12439)
                     /gene="folB"
                     /locus_tag="ACHUPB_001410"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0758268.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12544..13161
                     /gene="plsY"
                     /locus_tag="ACHUPB_001411"
     CDS             12544..13161
                     /gene="plsY"
                     /locus_tag="ACHUPB_001411"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0758269.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWSVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13174..14106)
                     /gene="ttdR"
                     /locus_tag="ACHUPB_001412"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13174..14106)
                     /gene="ttdR"
                     /locus_tag="ACHUPB_001412"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            14313..15224
                     /gene="ttdA"
                     /locus_tag="ACHUPB_001413"
     CDS             14313..15224
                     /gene="ttdA"
                     /locus_tag="ACHUPB_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR0758270.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15221..15826
                     /gene="ttdB"
                     /locus_tag="ACHUPB_001414"
     CDS             15221..15826
                     /gene="ttdB"
                     /locus_tag="ACHUPB_001414"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0758271.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15875..17338
                     /gene="ttdT"
                     /locus_tag="ACHUPB_001415"
     CDS             15875..17338
                     /gene="ttdT"
                     /locus_tag="ACHUPB_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0758272.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17381..18394)
                     /gene="tsaD"
                     /locus_tag="ACHUPB_001416"
     CDS             complement(17381..18394)
                     /gene="tsaD"
                     /locus_tag="ACHUPB_001416"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0758273.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18631..18846
                     /gene="rpsU"
                     /locus_tag="ACHUPB_001417"
     CDS             18631..18846
                     /gene="rpsU"
                     /locus_tag="ACHUPB_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0758274.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18957..20702
                     /gene="dnaG"
                     /locus_tag="ACHUPB_001418"
     CDS             18957..20702
                     /gene="dnaG"
                     /locus_tag="ACHUPB_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0758275.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20897..22738
                     /gene="rpoD"
                     /locus_tag="ACHUPB_001419"
     CDS             20897..22738
                     /gene="rpoD"
                     /locus_tag="ACHUPB_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0758276.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5520 a         6226 c         5839 g         5219 t
ORIGIN      
        1 taacaaagat aaatgtaagc aaggaaacta tcccaaagta aatattgcgt cactaaatgg
       61 acattggagt gacatatgat tttattcgcc gactataata ccccttatct tttcgctatt
      121 tcctttgtac tgttgatcgg ccttttagaa attttagcgc ttatctgcgg tcacatgctc
      181 tccggtgctc ttgatgcaca tcttgaccat tataattcca taaccacagg tcatattagc
      241 caggcacttc attatctcaa tatcggaaga ttgccagctc tcgtcgttct ctgcttgctg
      301 gctggttttt ttggtcttat tggcatcctg ttgcagcatg cctgcatcat ggtctggcaa
      361 tcaccactgt cgaacctgtt cgtggttcct gtcagtttac tgtttacgat tattgcggtg
      421 cattataccg gtaaaatagt tgcgccctgg attccacgcg accatagttc agctattaca
      481 gaagaagaat atattggtag catggcatta attaccggtc atcaagccac ttcaggtaat
      541 ccctgcgaag gaaaactcac cgatcaattt ggccaaatac attatttact actggagcct
      601 gaagaaggaa aaattttcac taaaggagtc aaagtgttaa ttatttgccg actttctgca
      661 acgcgatatt tggcggaaaa caatccctgg ccacaaatat tgtaatttat taatcaaaag
      721 gaaatttaat aatggatgat attgttaatt ctgtgccctc ctggatgttt accgcgatta
      781 ttgccgtatg cattctgttt attattggaa ttattttcgc caggctctat cgtcgcgctt
      841 cggcagagca agcttttgtt cgtactggtt taggtgggca aaaagtggtg atgagcggtg
      901 gcgcaatcgt gatgccgatc tttcatgaaa taatccccat caatatgaat actctgaagc
      961 tggaagtcag ccgctcaacc attgatagcc tgattacgaa agatcgtatg cgtgtcgatg
     1021 tagtagtcgc tttctttgta cgggtaaaac cgtctgtaga aggcatcgcc actgcggcgc
     1081 agacgttggg gcaacgcacg ctatcaccag aagacttacg tatgttggtt gaagataaat
     1141 ttgtcgatgc cctccgtgca acagctgcac aaatgaccat gcatgagtta caggataccc
     1201 gcgagaactt cgtgcaggga gtacaaaata ctgtcgcaga agacctatcg aaaaacggtc
     1261 ttgagctgga aagcgtttca cttaccaact ttaaccagac ctcgaaagaa catttcaatc
     1321 cgaacaatgc ctttgacgcc gaaggtttaa ccaaactgac tcaggagacg gagcgccgtc
     1381 gtcgcgaacg taacgaagtt gaacaggatg tagaagttgc ggtgcgtgag aaaaaccgtg
     1441 atgcactttc gcgcaagttg gagattgaac aacaagaagc gtttatgacg cttgagcagg
     1501 agcagcaggt taaaacccgt actgccgaac agaatgcacg tattgcggct tttgaagctg
     1561 aacgtcgtcg tgaagcagag cagacgcgaa ttctggctga acgacaaatt caggaaacgg
     1621 agatcgagcg cgagcaggcc gtccgctcaa gaaaggttga ggctgaacgt gaagttcgta
     1681 ttaaagagat cgaacagcag caggtcaccg aaatcgccaa ccagacgaaa tcgatcgcta
     1741 ttgccgccaa atcggaacaa cagtcccagg cagaagcgcg tgctaacctt gcactcgcag
     1801 aagcagtaag tgcgcaacaa aacgtagaaa ccactcgcca gaccgcagaa gccgatcgtg
     1861 ctaaacaagt tgccctaatc gctgccgcgc aggatgcaga aaccaaagcg gttgaactga
     1921 ccgtgcgggc gaaagcagaa aaagaagccg cagagatgca ggcggcggct atcgttgagt
     1981 tagccgaagc tacacgtaaa aagggtctgg cggaagcaga agcacaacgt gcgctgaacg
     2041 atgctatcaa cgtactttct gatgaacaaa ccagccttaa attcaaactg gccttgttgc
     2101 aggcgctacc tgcggtaata gagaaatccg ttgagccgat gaagtccatt gacggtatca
     2161 agattattca ggtcgatggt ctgaatcgtg gcagcgctgc gggtgatgca aacacgggta
     2221 atgtgggggg cggaaaccta gcggaacaag cattatcagc cgctctctct taccgcacac
     2281 aggcaccgct gattgactcc ttgctcaatg aaattggcgt ttcaggcggc tcactggcgg
     2341 cattgacttc acccttaacc tcaacaactc ccgtcgccga aaacgtagaa taaattttca
     2401 gctatgaacg tccctggcac aaaaagccgg ggatttttta tatctgcgtt ccgctaaaaa
     2461 ctgcaaatga tcaggccgtt gcaacgtttt gcgtgaccgc tcggggaagg caaaactgcc
     2521 tctgggaaag cgttgcgcga ggtccggcgc tcatcaacaa tcggggagtc agcaaagggc
     2581 tgaaacggga aagcccctcc cgaggaaggg gccttacaga aggaaagggt tatgatgaag
     2641 ctcgtcatca tactgattgt gttgttactc gtaagtttcg cagcttatta acagccaatc
     2701 agagggggga gaaatcctcc ctcacccttg ttcctttact ttaggttgaa aaaaaatcag
     2761 cgtcaagaag gtttcattaa cacatatccc tggcccaaaa agccggggat tttttatatc
     2821 tgcgttccgc taaaaggtgc aaatgctcag gacgttgcag cgttttgcgt gatcgctcgg
     2881 ggaaggcaaa actgcttcag ggaaagcgtt gcgcggggtc cggcgctcat caacaatcgg
     2941 ggagtcagca aggggctgaa acgggaaagc ccctcccgag gaaggggcct tatagaagga
     3001 aagggttatg atgaagttcg tcatcatact ggttgtgttg ttactgttaa gtttcccgac
     3061 ttactaacaa ctgatcagag ggggaagaaa tcctccctta cccttgttcc tttactttag
     3121 gttgaaaaaa caacagcgtc aataggcctg cctgctacaa agcgagatct gtgaaccgct
     3181 ttccggttag ccttttttat cctgttggat cttcttgatg atgttagtgg tcgagcaacc
     3241 gtcttcgaag ttgagcacca gcacttcgcc gccgttggcc cagacttctt tactcccggc
     3301 gatctcttct ggtttatagt cgccgccctt caccagtaaa tctggcagga tcccggcgat
     3361 caagcgctgc ggcgtgtctt cttcaaacga caccacccag tcgaccgctt ccagtgcgcc
     3421 cagcacaatc atacgctgtt cgagcgggtt aacggggcgg gaatcccctt tcagccgttt
     3481 ggtggaggca tcgctgttca cagcgacaat cagacggtca ccctgtttgc gagcatttgc
     3541 cagataggag acgtgcccgg cgtgcaggat gtcaaagaca ccgttggtca ttaccacctt
     3601 ttcgccgcgt ttacgcgctg ccgctacggc cagcttcagt tcctcttcgg tcatcacgcc
     3661 aaagccggta tctgcgcgcc cacgcactgc attttccagc tcgatcggcg aaaccgtgga
     3721 ggttcccagt ttgccgacca ccacgccagc cgccgcattg gcaaagaagc aggcttcttc
     3781 cagcgaatta cccgctgcca gcgttgccgc caggacgcca atcaccgtgt cgcccgcacc
     3841 ggtaacgtca tacacttcct gcgcctgggt tggcatatgc agcggcgctt tacccggttg
     3901 cagcagcgac ataccctgtt cggaacgggt cactaacaga gccgagagtt cgtaatcggc
     3961 aatcagtttc atgccgcgct caacaatctc ttcttcggtc ttacatttac cgacaacagc
     4021 ttcaaattcc gagagattcg gcgttaacag cgtagcgccg cggtagcgct caaaatcggt
     4081 accttttgga tcaatcagca ccggaacgcc cgctttacgc gccagttgga tcatctgctg
     4141 tacgcttgcc agcgcacctt tggcgtagtc agaaagcacc agcgcgccaa tcgaactcag
     4201 cgcctgatta atccgctcgt gcagcggctg cggatcaacg ccttcgaaac cttcttcgaa
     4261 atccagacgg atcagctgtt ggttgcggga aagtacccgt aatttggtga tggtcggatg
     4321 cgtcggtaca gaaacgaagt cgcatttgac gttgacgtcg gccagagatt tactcagcgc
     4381 gcgcgctgca tcgtcaatgc ccgtcaaccc gaccaggcgt gcattagcac cgagagaagc
     4441 gatattcatc gccacgttag ccgcgccgcc cggacgttct tcgatggtat tcactttaac
     4501 cacgggcacc ggcgcttccg gcgagatacg gctggaggga ccgtaccagt aacgatccag
     4561 cattacatca ccaaccacca tcactcctgc acgttcaaac tctggcagcg ttactttcat
     4621 tcctgtctcc tgagagattc aaaatttgcg cgcgataata ccatacttca ttcttccacc
     4681 agccacttct gccagcttgc ccgcaccaga tcacgctctg cggtgaagca atcccccgac
     4741 acatggcccg gcaattcctg taatgccaga tgatgaagtt catcgcgcag cgtcgtgtaa
     4801 gcacgggtca gcgccatcgc ttcctgctct tccataatgt cgttttgcgc cagcagctcc
     4861 agaatacgca cgttgtctga ccagcgcgtt aacttcggtt tttcatgagc gtagcgcaac
     4921 accagatatt gggtaataaa ttcgatatcg gtaattccgc cttcatcagc tttgatatca
     4981 aagcgatcgc gatgtttatt gccgagatga gcgcgcattt tctcgcgcat ttcccgcact
     5041 tccgtttgca gagttctacc ttcacgcggc agcgtcataa tctcgcgacg cactgcgtca
     5101 aagtgcgcgg tgagctgcgg atcgccgtac actacccgcg cacgcaccag cgcctgatgt
     5161 tcccacgtcc aggcctcgtt tttctgataa tcggcaaatg cttctgtgga ggtaaccagc
     5221 attcccgccg ccccggacgg gcgcagccgc gcatccactt cgtacaaaat gccggaagag
     5281 gttcgcgtgc tgaacaggtg cataatgcgt tgcgccagac gaagataaaa ctgtcgtccg
     5341 tcgatttccc gctcgccgtc agtcatcgca tccattgggc aatcatggag gaagatcagg
     5401 tcaagatcgg aactgtagcc taactcccag ccgcccagct tgccgtagcc gaccactgca
     5461 aaaccacgcc cttcgcgatc gttgaggtga ttcggcttac cgtagcgggc aaccatttga
     5521 acccacgcct gctgaacgac ggcatctatc atggcttccg ccagccaggt taagtgatcg
     5581 ctcactttca tcaccggtag cgtaccagcg atatccgctg cggcgatgcg taacagctgc
     5641 gcctgtttga actgacgcag cgcctcaagc tgttgctctt cgtcatcttc cggcacgcgc
     5701 agcaaatact ggcgcaactc atcgcggtag gcatcggtcg ccgtcggctg gtaaagagtg
     5761 tttggatcga gcaattcatc cagcaataat ggataacgcg ccagctggct ggcaatcatc
     5821 ggcgatgcgg cacacagaga aatcaaatgg ttaagcgccg cagggaattc actgagcaat
     5881 tctaaatagg tggtgcgggt aacaatcccc accagcaagg cggtaatgcg cgacagcgtg
     5941 acagcagcgt cttcacgcgc acagacatca cttagcagat gcggcatcag atggtcgagc
     6001 acctgacgtc ctcgcggccc gatggtgcgc ttatccagct ctttgcggaa atcggaaatc
     6061 agcgttagca cctgtttgcg atcatcctcg ctaagatgcg ccagcactgg cgtagtgtca
     6121 tcttcctgca acgcatcctg ccacagctca cgccactgtt ccgacagcga ctcttcctga
     6181 gtttcacttt cgtcgtcgcc aatcaattca ttaaacaccc ggcgcacatt gttcatatgt
     6241 gcggtcagcg ccccggtcag ttgcggccag tcagcaaagt ccatcgccca cgccagccgc
     6301 gcacggttaa gctcatcaga aggaagcgtc tgggtttgtt cgtcgttaat gctttgcagc
     6361 aggttttcca gacgccgcag gaacagatac gccactcgca attgttcagc atcgttttca
     6421 gaaagcagat gcagcgcagc aatggcgctg agcgttggca gtaaagagca cgattgcagc
     6481 gacggttcgc gcccgccgcg aatgagctgg aatacctgga cgataaattc aatttcgcga
     6541 ataccacccg cgccaagttt gatgttgtcg gtcaaaccgc gccgacgcac ttcacgggca
     6601 atcattcctt tcatgttgcg cagcgactga atcacgctga aatcgatgta acgacggaaa
     6661 acaaacgggc gtagcatcgc acgcaactcg ttagcataga cgccttcgct atcgcccata
     6721 atccgcgctt tgaccatcgc gtaacgctcc cagtcgcgcc cctgctcctg gtaataatcc
     6781 tccaacgcgg caaagctcag caccagcggg ccactttcgc caaacggacg cagtcgcata
     6841 tccacgcgat agacgaagcc atcctgcgtt ggttgatcca gcactttaat cagccgctgc
     6901 cccatgcggg taaaaaactg cgcgttatcc agttcccggc gtccgccctg cgtacaaccg
     6961 tgttccggcc aggcaaaaat cagatcgata tcagaggaga aattcagttc cccgccgccc
     7021 agcttaccca tgcctaagat cagcagcggt tgcgcttcgc cctgcgcatt gcacggcgtt
     7081 ccccactcgc ggcagcaggc gtcatacagc cagtcacgcg ccgcaacaat cagcgtctcc
     7141 gccagataac tgagctgctg caatatgctc tcttcagtaa ccagtgccag cgtttgcgcc
     7201 catgcgatgc gcaccataat gcgccgccgg aatagccgca gctcgcgcat taacccggct
     7261 tcgtcactca cattactgag cgcctcctgc aaccatgcag cgtaatgctg ccattcatcg
     7321 gcctgcggcg gttggctttc cagttccgtc aaccactctc gatgcgcaat tatgctgtcc
     7381 tgcacaaaat cactgaaagt aagtactgac ttcgcctgtg cacttagtgg ttcctcggct
     7441 aaaggctctg gcagccgctc aacaacggtc tgcctgtact gctgtaacgg tgaagagagc
     7501 ggcttcataa gcgattttat ccttgcctga ttaacgtttt ccgctgtgca accagaacgg
     7561 ttcctgattg tttgcctcat tacggaaatg ctcaatttca atgcgttgcc cggtcgcaat
     7621 agcgtgacgc agcccctgcc agttctccag ccaggcatgc gcgacgacag gatcatagta
     7681 acccgccagc aacagtattg agtcaatgtc acgcgtcagg cgtggcaact ggtcacggta
     7741 gagatcgcct aacggctggc agaaaacgct tttcagttca gcggcatggt gggaaagatg
     7801 gatatcggca aagcgtttga aggagtcgct gattttgccc tgcgctttgg catctaaaaa
     7861 tggctgccat gctttgctta ccaaccattc ggtcaacgcc agcttcgcca ttgccgtttc
     7921 ggtagagtag accgccgtca cggcagaaac cgcagaagca atggtcgctt cgcattgggt
     7981 cagcaaatca cgtaagtgag tgctcgcttt acgcggcaca ataccaccga acagcatcag
     8041 cgtatgacgg accaggctaa tggctgccag cacctgttct ttcgccgcat cgttaccgcg
     8101 tacccacagt tcttcatgat actgccattg cgctaacgcc agctcgagcg ccgcttccag
     8161 cccctgttct acatcggctt ttgccgcaac atgcaaaatg gtggtcggtt tgatttcacg
     8221 cgccggattg ccctgcgcca gatgatagcc acgcgccgct ttgctcaggc tgccctggcg
     8281 taatccggtt tgcgatacca gttggttcgc cagtttcagc accgcgcgcg tgtcgccgct
     8341 aagcagttcc agttccagct cacagatagg ttcagcaaat tcccccgctt tcacctcccc
     8401 ctgatcgaga gcgatttcaa ttcggctgcc atcgaccgcc accagccatt tttcgcgata
     8461 aaaatcggtg ctgaacagcg gttgtacgcg ggaagcgaga tcggcaggca attcaccgtt
     8521 cggccagact tccgagggaa actgcgccag gtcgagcgtc ggttcgctca acgccacgtt
     8581 atattccggg cgctggtgta agccgcctgt cactcttcct gcaactttca tggtcatctc
     8641 atagcgaccg ttttcgccac gaatacgtaa gcccatatcg tgcctacgca gccagttatc
     8701 cggcgtttcg tagtaaatat tcagcaactg cacggggtca tggtgctcgc cgcccagcgt
     8761 attgagatgg tcacgcaacg cctcaacggc actgtgatta acaataaact ttaattcgat
     8821 ttcctgagcc atggccttta cttatcggtt atgtcacata agtgacgatg aacggcgaac
     8881 ttaatgcgat cttttttgtc agtagatagt attttgcgcc aaattgccat gcaacgggca
     8941 atttgacggg cgtaaaagtt tgaagcagtg gcaagacctg acacagagga ttccgattga
     9001 tgtcgaatgc tttgcgtagc gacactgatg ccactactat cgttccactt tccatgacaa
     9061 taacgacagc ctgatgccaa aattacgcct gatcggatta actttactcg cacttagcgc
     9121 gactgccgtc tcacacgctg aagaaacgcg ctatgtttct gacgaactga atacctgggt
     9181 ccgtagcggt ccgggagatc attatcgcct cgtgggcacg gttaacgccg gcgaggaagt
     9241 gaccttatta caaactgacg ccaacaccaa ttatgcccag gtgaaagaca gctctggccg
     9301 taccgcctgg atcccgttga aacaacttag cactgagcca agcctgcgct cccgtgtgcc
     9361 agatctggaa aatcaggtca aaaccctgac cgataaactc accaatatcg ataacacctg
     9421 gaatcagcgc accgcagaaa tgcagcaaaa agtggcgcag agcgacagcg tgatcaacgg
     9481 gttaaaagaa gaaaatcaga aactgaaaaa cgagctgatt gtcgcgcaga aaaaggtcga
     9541 tgccgccagc gtacagctgg atgacaaaca gcgcaccatc atcatgcaat ggtttatgta
     9601 tggcggtggc gtgctggggc ttggcttgct gctcggtctg gtactgccgc acctgatccc
     9661 aagccgcaaa cgcaaagatc gctggatgaa ctaaatcgcc ttcttcgttg cactgtctta
     9721 ttattaggaa ttgtgtattt gacgaaggga tgatggcgtg aagatttatc tggtcggtgg
     9781 tgctattcgg gatgcattgt tagggctacc ggtcaaagac agagattggg tggtagtcgg
     9841 cagtacgcca caggagatgc tcgacgcggg ctaccagcag gtaggccgcg attttcctgt
     9901 gtttctacat ccgcaaacgc atgaagagta tgcgctggca cgtaccgaac ggaaatccgg
     9961 ttccggttac accgatttta cttgctatgc cgcaccggat gtcacgctgg aagatgatct
    10021 taagcgtcgc gatctgacca ttaatgcgct ggcccaggac gataacggcg agattatcga
    10081 cccgtacaac ggtctgggcg atctgcaaaa tcgtctgttg cgccatgttt cccccgcttt
    10141 tggcgaagat ccgttacgcg tactgcgcgt ggcgcgtttt gctgcgcgtt atgcccacct
    10201 cggttttcgt attgccgatg aaactctgac gctgatgcgc gagatgaccc atgcgggtga
    10261 actggaacac ctgacgcctg aacgggtatg gaaagagacg gaaagcgccc tcaccacccg
    10321 caatccacag gtgttcttcc aggtactgcg cgattgcggc gcactgcgcg ttttattccc
    10381 ggaaattgac gcactgtttg gcgttccggc ccctgccagg tggcatccgg aaatcgacac
    10441 gggtattcat accttaatga cgctctcaat ggcggcgatg ctgagtccgc aggtcgatgt
    10501 ccgtttcgca actttatgcc acgatctcgg taaaggactg acgccgccag aactttggcc
    10561 gcgtcatcat ggtcacggtc cggcgggtgt gaaattagtg gaacaactct gccagcgtct
    10621 gcgcgtgcca aatgaaattc gcgatttagc cagactggtg gctgagtttc acgatctcat
    10681 ccacaccttc ccaatgctga acccaaaaac tatcgtcaaa ttgtttgatt ccatcgacgc
    10741 ctggcgtaaa ccgcagcgtg tcgagcaact ggcgctgacc agcgaggctg acgtacgcgg
    10801 cagaaccggt tttgaatcag cggactaccc gcaaggccgt tggttgcgcg aagcctggga
    10861 agtggcgcag tcagtgccga caaaagcggt cgttgaagcg ggatttaaag gtgtggagat
    10921 tcgcgaggag ctgacccgac ggcggattgc ggcggtagcc agttggaagg aacaacgttg
    10981 cccaaagcct gactgagtat aaaactaaac gttaacacga accatcccct cgccccttcg
    11041 gggagagggt tagggtgagg ggaacagacc agcaccggtg cgaacaatca cccccacccc
    11101 ggccctctcc ctacagagcg agggagaaaa ccaggcctgc tcccgaacct tttctcaatc
    11161 aaaagaacac gacgtacacc gcagccgcca caataaagcg ataaatggcg aacgggataa
    11221 acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc tatcagcgcc accacaaaag
    11281 cggtgataaa cccaacggca aacatcgaga tatcgccggt tgtcaggaag ccccagcttt
    11341 tgtagagatc gagcgccgtt gcgcccatca tcatcggtac cgccagcagg aacgagaact
    11401 cggaagcagc gtaacggctc acgcccatca gcatcccacc tgaaatggtg gccccggaac
    11461 gggagaaacc cggccacagc gccagacact ggaaacagcc aatcataaac gcctgacgat
    11521 aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg cttcaggcat tcggcggcaa
    11581 tcagcaacaa accgccaacg accagtgcat acatcacatt tatcgggtta aacaatgact
    11641 taatcgtgtc gtggaacaac agccccagta ccaccgccgg aatcatcccc agcaaaatgt
    11701 ggatcagcgt taaacgacct ttgctttctc cttcgtgctg caacgggcgg ccaaagtgga
    11761 tgccaatcag gccaaacaga cgccgccaga acatcactac taccgccaga attgatccta
    11821 actggatcac aacttcaaag gttttcgccg tgtcgccctc gaaccccaac aagtgaccga
    11881 caataatcat atggcccgtg ctggataccg gcagaaattc tgtcaatcct tcgaccacac
    11941 ccaatattgc cgctatcagc agcgagtgca tatcgctcat caataaaccc ctaaattatt
    12001 aaaatgtacc gcttgtccgg actactgcag taagaccagg ttataaccgt ttggtttaac
    12061 agctgtaaaa ttaattattt tctttcagat tattgccacg ctcaatgatt acaccaacat
    12121 tcgccgcccg cgccattgcg cctggcttgc tgagtttgat gcgcacccac ggcgagttga
    12181 agcgtgctaa cagcagctcc gccacctctt cagccacgcg ttccaccagc gcaaaacgcg
    12241 ccccctcgac gtggctgacc accgtttctg caatgtcagc gtaactgagg caatccgcca
    12301 catcatcact tttcgccgct ttacggttat cccatgccat ttcgatatcg aacactaact
    12361 tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt ggtgattacc gaaagttgct
    12421 ctataaatac aatatccatc acgtcctgcc tgctttttgg ctaaccggat accacttccg
    12481 gcgaaatgtg cgtattatcc acagattcat cgttgaacac gaattttcaa aacggaacag
    12541 cttatgagtg caatcgcgcc tggaatgatc ctcatcgcgt acctctgcgg ctccatttcc
    12601 agtgccattc tggtttgccg cttgtgtggg ctgcccgatc cgcgaaccag cggctccggc
    12661 aatccaggcg caaccaatgt gttacgtatc ggtggcaagg gagcagccgt agcagtactg
    12721 attttcgacg ttctgaaagg aatgttgccc gtctggggcg cgtatgaatt aggtgtcagc
    12781 cccttctggt taggcttaat tgccatcgcc gcctgtcttg gacacatctg gtccgttttc
    12841 ttcggattta aaggaggaaa aggcgttgct accgcttttg gtgccatcgc acccattggc
    12901 tgggatctca ccggagtaat ggcgggaacc tggttactga ccgtgctatt gagcggatac
    12961 tcgtcgctgg gagcgattgt cagtgcactg attgctccgt tttatgtctg gtggtttaag
    13021 ccacaattca ccttcccggt ttcgatgctc tcttgcctga tcctgctgcg tcatcatgac
    13081 aacatccagc gtctgtggcg tcgtcaggag acaaaaatct ggacgaaatt caaaagaaag
    13141 cgcgaaaagg atcccgagtg atttctggtg gatctacatg acctgatagc cttcatcggg
    13201 cttgcccagc cgttgctggc accatgccgc cagaaattcc acgcagacac gtaatttcat
    13261 gctgcgatag agcggctccc ggtaaacagc ccagatattg gcgctttgtg catactccgg
    13321 caatacttgc accagtttgc cactctccag aaacggcaac acatcccact cggaacgcag
    13381 cataatccct ttgccctcca gcgcccattg caacactatc tcgccgctat tggaggaaag
    13441 atgcccgcta accttcaccg attttttctc ctgaccgttc cccaactccc atattccgtg
    13501 ggtcatatcg cgctctttgg tcaccagaca gtcatgacga cttaattcct gtaatgattg
    13561 tggctgcgga tatttttgca gatactcagg tgctgcgcat aatattcttt tatttttcgt
    13621 taacagatgc gcaatataat aatcaggaat ttcgtcatta atacgaatat ccagatcaat
    13681 attatcctgc actaaatcaa tttgccgatc gaacaattca aaatgcacct gtaactcagg
    13741 ataattgcgc ataagttcgg taatggctgg cgcaatatgg ctgcgcccaa aaccaaaact
    13801 gcaaccaata cgaatcatcc cctccgggcg cgttttgatt tacgtgacgt catcgaccag
    13861 tcgctgatat tgggtgagga tttccagcgc atgttcgtaa caacgttggc cactttcagt
    13921 cagcgccacg ccgcgagcgg agcggttcag cagcgttgtt gccagcgtat tttcaaggat
    13981 ttggatccgc ttggtgacaa atgcaggcgt ctgcccaagc gtcgccgctg cggcgctaaa
    14041 actcccgctg tggacgattt ctaccagtac ctgcaagtct ttggctaaag gccagctatt
    14101 cagcatcgtt aattatccgc agttgtgata agcgcagtgt atttcgcaaa acattgccat
    14161 tccctgctct ggcgcaatat tcattcttat tttcgcgttg attcacgaat tgttaattac
    14221 atattcacgg tggcaaaaaa tataaaacca catttttagt ggtagtttgt ggcggtgaat
    14281 ttttccagac aaatacaaaa actggagttg ccatgatgag cgaaagtaat aagcaacagg
    14341 cagtgaataa gttgacggag attgtcgcta actttaccgc catgatttct acccgaatgc
    14401 ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc cgaaacgtcg tcgatgggga
    14461 aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc gattgacctg aatcgtcctg
    14521 cctgtcagga caccggggag attatgttct tcgttaaagt cggttcccgc ttcccactgc
    14581 ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga ggcaaccgtc aaagcgccac
    14641 tacgtcacaa tgcggtagaa atttttgacg aagtaaacac cggcaaaaat accggtagcg
    14701 gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga cgatgcggaa atcgaagttt
    14761 acatggcagg cggcggctgc acgctacctg gccgctcgaa agtgttaatg ccgtcagaag
    14821 gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc caccctcgcc gtaaacgcct
    14881 gtccaccggt actggtgggc gtgggcatcg ccacctcggt ggaaaccgcc gccgtactct
    14941 cgcgtaaagc cattttgcgc ccgattggca gccgccatcc caatccaaaa gcggcagaac
    15001 tggagctacg cctggaagaa ggactcaacc gtctggggat tggtccacaa gggctgactg
    15061 gcaacagttc agtgatgggc gtacatatcg aatctgccgc ccgccatccg tcaaccatcg
    15121 gcgttgctgt ctctaccggc tgctgggcgc atcgtcgcgg cacactgctg gttcatgccg
    15181 atctcacctt tgaaaatctg tctcacaccc ggagcgcgtt atgaaaaaga tcctgacaac
    15241 cccgatcaaa gctgaagatc tgcaagatat tcgcgtcgac gatgtgatct acctgaccgg
    15301 tacgctggtg acctgccgtg acgtttgtca ccgccgcttg atcgaactga aacgtccgat
    15361 cccttacgat ctcaacggca aagcgatttt ccacgctggc cccatcgtgc gcaaaaacgg
    15421 cgataaatgg gagatggttt ccgttggccc gacaaccagt atgcgtatgg aaagttttga
    15481 acgtgaattt atcgagcaga ccggcgtgaa actggtggtt ggcaaaggcg gaatggggcc
    15541 gctgaccgaa gaaggctgcc agaaattcaa ggcgctacat gtgattttcc cggcaggctg
    15601 cgcggtgctg gcggcaaccc aggtggaaga gattgaagaa gtgcactgga cagagctcgg
    15661 aatgccggag tcgctgtggg tctgtcgggt caaagagttc ggcccgctga ttgtctctat
    15721 tgatacccac ggcaacaacc tgatagccga aaacaaaaag ctgttcgccg aacgccgcga
    15781 tcccatcgtg gaagagatct gcgagcacgt ccattacatc aaataaccct cccggagagg
    15841 ctcgccccct ctcctttttc gcaggcataa cacgatgaaa ccttccactg aatggtggcg
    15901 ataccttgcg ccgctggcgg tcatcgccat tattgctcta attccggttc ccgcagggct
    15961 ggagagtcat acctggctct actttgccgt ttttactggc gtgatcgttg gactgatcct
    16021 cgaacccgtg ccgggtgccg tggtggcgat ggtgggtata tccatcatcg ccatactctc
    16081 tccctggctg ctgttcagcc cggagcagct cgctcagcca ggctttaaat tcactgcaaa
    16141 atccctctcg tgggccgttt ccggtttttc taattcggtt atctggctga ttttcgccgc
    16201 ctttatgttt ggcacaggct atgaaaaaac cgggcttgga cgccgcattg cgctgattct
    16261 ggtgaaaaag atgggacatc gcacgctgtt tctcggctat gcggtgatgt tctccgagct
    16321 gatcctcgca cctgtaacac cgtccaactc ggcgcgtggt gcggggatta tctatcccat
    16381 catccgtaac ctgccaccgc tctatcaatc acaaccaaac gacagcagtt cgcgcagcat
    16441 tggctcgtac atcatgtgga tggggattgt tgccgactgc gtgaccagcg ccattttctt
    16501 gacggcgatg gcaccaaact tgctgttaat tggactgatg aaaagcgcat ctcacgccac
    16561 gctgagttgg ggcgactggt tcctcgggat gttgccgctc agtattttgc tggtgctgct
    16621 ggttccctgg ctggcttatg tgctgtaccc gccggtactg aagtctggcg atcaggtgcc
    16681 gcgctgggca gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat
    16741 gctggggctg atagtaggcg cgctggtgct gtggattttc ggcggtgatt atatcgatgc
    16801 cgcgatggtc ggttacagcg tggtggcact gatgctgctt ctgcgcatta tcagttggga
    16861 cgacattgtc agtaataaag cggcgtggaa cgttttcttc tggctggcct cgcttatcac
    16921 cctcgctacc ggactcaaca acaccggttt tattagctgg tttggcaaac tgttagcagg
    16981 cagcttaagc ggttattcgc caacgatggt gatggtggcg ttgattgtgg tgttttatct
    17041 gctgcgctac tttttcgcca gcgccacggc gtatacctcc gccctcgcac cgatgatgat
    17101 tgccgctgcg ctggcgatgc cggaaatccc gctgccggtg ttctgcctga tggttggcgc
    17161 ggcaattggt ctggggagca ttcttacacc atacgccacc ggacccagtc cgatttacta
    17221 cggtagtggt tatctgccaa cggcggatta ctggcgactg ggggcgattt ttgggctgat
    17281 attcctcgtg ttgctggtga ttaccggctt actgtggatg cccgtggtgt tgctttaagt
    17341 gaggacggtg ccggaaattg ctctccggca cccaatagtt ttacgcagcc ggtaactccg
    17401 ccagcggcca gcgcggacgc acgctaacgc cgagatccgc cgtcgcgcct gctttaaacc
    17461 gcaccattcc ggcataggcg atcatcgcgc cgttatcagt acaaaactcc ggacgcgcgt
    17521 agaacacttc gccgcggcgt tttttcatca tttcagccag cttcgcccgt aacgtgcggt
    17581 tagcactcac gccgcccgcc atgaccagtc gcttaaagcc cgtctgatcc aacgctcgct
    17641 tacacttaat catcaacgta tcgaccaccg catcttcaaa ggcgcgggcg atatcagcac
    17701 gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc cgcaaaggtt ttcagaccag
    17761 aaaagctgaa atccagcccc ggacggtcgg tcatcggacg cgggaaaaca aagcgcccgg
    17821 cagtaccctg agccgccatt ttcgacagta acggtccgcc aggataatcc agccccagca
    17881 gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc gatagactcg ccgagcagct
    17941 cgtactgacc aatgccagtc acgctgatta actgcgtatg accgccggac accagcagcg
    18001 cgacaaacgg aaattccggc gggttatctt ccagcatcgg cgctaacaga tgcccttcca
    18061 tatggtgtac cgggattgcc ggaacgttcc aggcaaacgc cagagaacgc cccacggttg
    18121 cgccaaccag cagtgcgccg actaatccag ggcctgcggt ataggccaca gcatcaatgt
    18181 cttttgccgt taagccagac tcctttagcg ccgcctggat caacggtacg gttttgcgca
    18241 cgtgatcgcg ggaggccagt tcaggcacga cgccgccata gtcagcgtgc aatttcacct
    18301 gactatacaa ttggttggct aacaaacctt tttcatcgtc gtaaatggcg atgccggttt
    18361 catcgcagga agtttcaata cccagtacac gcatgacttg ttttacctcg ctttattacc
    18421 gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg agaaagcctc
    18481 gtgtatactc ctcaccctta taaaagtccc tttcaaaaag gccgcggtgc tttacaaagc
    18541 agcagcaatt gcagtaaaat tccgcaccat tttgaaataa gctggcgttg atgccagcgg
    18601 caaaccgaat taatcaaagg tgagaggcac atgccggtaa ttaaagtacg tgaaaacgag
    18661 ccgttcgacg tagctctgcg tcgcttcaag cgttcctgcg aaaaagcagg tgttctggcg
    18721 gaagttcgtc gtcgtgagtt ctatgaaaaa ccgactaccg aacgtaagcg cgctaaagct
    18781 tctgcagtga aacgtcacgc gaagaaactg gctcgcgaaa acgcacgccg cactcgtctg
    18841 tactaattcc ccgagagcgt tgctctccga tcagaccgag ttgtagttgt aaggccgtgc
    18901 ttccgaaagg aatgcgcggc ttattttcgt ttatgaattg ctaaaaatcg gggcctatgg
    18961 ctggacgaat cccacgcgta ttcattaatg atctgctggc acgcactgac atcgtcgatc
    19021 tgatcgatgc ccgtgtgaag ctgaaaaagc agggcaagaa tttccacgcg tgttgtccat
    19081 tccacaacga gaaaaccccg tcattcaccg ttaacggtga gaaacagttt taccactgct
    19141 ttggatgtgg cgcgcacggc aacgcgatcg acttcctgat gaactacgac aagctcgagt
    19201 tcgttgaaac ggtcgaagag ctggcagcaa tgcacaatct tgaagtgcca tttgaagcag
    19261 gcagcggccc cagccagatc gagcgccatc agaggcaaac gctttatcag ttgatggacg
    19321 gtctgaatac gttttaccaa caatctttac aacaacctgt tgccacgtct gcgcgccagt
    19381 atctggaaaa acgcggatta agccacgagg ttatcgctcg ctttgcgatt ggttttgcgc
    19441 cccccggctg ggacaacgtc ctgaagcggt ttggcggcaa tccagaaaat cgccagtcat
    19501 tgattgatgc ggggatgttg gtcactaacg atcagggacg cagttacgat cgtttccgcg
    19561 agcgggtgat gttccccatt cgcgataaac gcggtcgggt gattggtttt ggcgggcgcg
    19621 tgctgggcaa cgataccccc aaatacctga actcgccgga aacagacatt ttccataaag
    19681 gccgccagct ttacggtctt tatgaagcgc agcaggataa cgctgaaccc aatcgtctgc
    19741 ttgtggtcga aggctatatg gacgtggtgg cgctggcgca atacggcatt aattacgccg
    19801 ttgcgtcgtt aggtacgtca accaccgccg atcacataca actgttgttc cgcgcgacca
    19861 acaatgtcat ttgctgttat gacggcgacc gtgcaggccg cgatgccgca tggcgagcgc
    19921 tggaaacggc gctgccttac atgacagacg gccgtcagct acgctttatg tttttgccgg
    19981 atggcgaaga ccctgacacg ctggtacgaa aagaaggtaa agaagcgttt gaagcgcgga
    20041 tggagcaggc gatgccgctt tccgcatttc tgtttaacag cctgatgccg caagttgatc
    20101 tgagtacccc tgacgggcgc gcacgtttga gtacgctggc actgccattg atatcgcaag
    20161 tgccgggcga aacgctgcga atatatcttc gtcaggaatt aggcaacaaa ttaggcatac
    20221 ttgatgacag ccagcttgaa cgattaatgc caaaagcggc agagagcggc gtttctcgcc
    20281 ctgttccgca gctaaaacgc acgaccatgc gtatacttat agggttgctg gtgcaaaatc
    20341 cagaattagc gacgttggtc ccgccgcttg agaatctgga tgaaaataag ctccctggac
    20401 ttggcttatt cagagaactg gtcaacactt gtctctccca gccaggtctg accaccgggc
    20461 aacttttaga gcactatcgt ggtacaaata atgctgccac ccttgaaaaa ctgtcgatgt
    20521 gggacgatat agcagataag aatattgctg agcaaacctt caccgactca ctcaaccata
    20581 tgtttgattc gctgcttgaa ctgcgccagg aagagttaat cgctcgtgag cgcacgcatg
    20641 gtttaagcaa cgaagaacgc ctggagctct ggacattaaa ccaggagctg gcgaaaaagt
    20701 gatttaacgg cttaagtgcc gaagagcgat cgggaagccc ccgacagccg cactgagagg
    20761 cagcggcaaa tatataagta cgccctcgta attatcgttg gcggtaaaca accgttggat
    20821 ttcagcgtta acggctgaag gacatcgggt caatcgccca acaccaacct catgaaataa
    20881 gtgtggatac cgtcttatgg agcaaaaccc gcagtcacag ctgaaacttc ttgtcacccg
    20941 tggtaaggag caaggctatc tgacctatgc cgaggtcaat gaccatctgc cggaagatat
    21001 cgtcgattca gatcagatcg aagacatcat ccaaatgatc aacgacatgg gcattcaggt
    21061 gatggaagaa gcaccggatg ccgatgatct gatgctggct gaaaacaccg cggacgaaga
    21121 tgctgccgaa gccgccgcgc aggtgctttc cagcgtggaa tctgaaatcg ggcgcacgac
    21181 tgacccggta cgcatgtaca tgcgtgaaat gggcaccgtt gaactgttga cccgcgaagg
    21241 cgaaattgac atcgctaagc gtattgaaga cgggatcaac caggttcaat gctccgttgc
    21301 tgaatatccg gaagcgatca cctatctgct ggaacagtac gatcgtgttg aagcagaaga
    21361 agcgcgtctg tccgatctga tcaccggctt tgttgacccg aacgcagaag aagatctggc
    21421 acctaccgcc actcacgtcg gttctgagct ttcccaggaa gatctggacg atgacgaaga
    21481 tgaagacgaa gaagatggcg atgacgacag cgccgatgat gacaacagca tcgacccgga
    21541 actggctcgc gaaaaatttg cggaactacg cgctcagtac gttgtaacgc gtgacaccat
    21601 caaagcgaaa ggtcgcagtc acgctgccgc tcaggaagag atcctgaaac tgtctgaagt
    21661 attcaaacag ttccgcctgg tgccgaagca gtttgactac ctggtcaaca gcatgcgcgt
    21721 catgatggac cgcgttcgta cgcaagaacg tctgatcatg aagctctgcg ttgagcagtg
    21781 caaaatgccg aagaaaaact tcattaccct gtttaccggc aacgaaacca gcgatacctg
    21841 gttcaacgcg gcaattgcga tgaacaagcc gtggtcggaa aaactgcacg atgtctctga
    21901 agaagtgcat cgcgccctgc agaaactgca gcagattgaa gaagaaaccg gcctgaccat
    21961 cgagcaggtt aaagatatca accgtcgtat gtccatcggt gaagcgaaag cccgccgtgc
    22021 gaagaaagag atggttgaag cgaacttacg tctggttatt tctatcgcca agaaatacac
    22081 caaccgtggc ttgcagttcc ttgacctgat tcaggaaggc aacatcggtc tgatgaaagc
    22141 ggttgataaa ttcgaatacc gccgtggtta caagttctcc acctacgcaa cctggtggat
    22201 ccgtcaggcg atcacccgtt ctatcgcgga tcaggcgcgc accatccgta ttccggtgca
    22261 tatgattgag actatcaaca aactcaaccg tatttctcgc cagatgctgc aagagatggg
    22321 ccgtgaaccg acgccggaag aactggctga acgtatgctg atgccggaag acaagatccg
    22381 caaagtgctg aagatcgcca aagagccaat ctccatggaa acgccgatcg gtgatgatga
    22441 agattcgcat ctgggggatt tcatcgagga taccaccctc gagctgccgc tggattctgc
    22501 gaccaccgaa agcctgcgtg cggcaacgca cgacgtgctg gctggcctga ccgcgcgtga
    22561 agcgaaagtt ctgcgtatgc gtttcggtat cgatatgaac accgaccaca cgctggaaga
    22621 agtgggtaaa cagttcgacg ttacccgcga acgtatccgt cagatcgaag cgaaggcgct
    22681 gcgcaaactg cgtcacccga gccgttctga agtgttgcgt agcttcctgg acgattaatc
    22741 ggtaggccgg atcaggcgtt acgccgcacc cggcactagt ccctctgcac aaacgccacc
    22801 tttt
//
LOCUS       DAZOZD010000045        22741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOZD010000045 DAZOZD010000000
VERSION     DAZOZD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.045"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..459)
                     /gene="folK"
                     /locus_tag="ACHUPB_001420"
     CDS             complement(<1..459)
                     /gene="folK"
                     /locus_tag="ACHUPB_001420"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0758277.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAF"
     gene            complement(456..1853)
                     /gene="pcnB"
                     /locus_tag="ACHUPB_001421"
     CDS             complement(456..1853)
                     /gene="pcnB"
                     /locus_tag="ACHUPB_001421"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0758278.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1913..2809)
                     /gene="gluQRS"
                     /locus_tag="ACHUPB_001422"
     CDS             complement(1913..2809)
                     /gene="gluQRS"
                     /locus_tag="ACHUPB_001422"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0758279.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2876..3331)
                     /gene="dksA"
                     /locus_tag="ACHUPB_001423"
     CDS             complement(2876..3331)
                     /gene="dksA"
                     /locus_tag="ACHUPB_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0758280.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3509..4213)
                     /gene="sfsA"
                     /locus_tag="ACHUPB_001424"
     CDS             complement(3509..4213)
                     /gene="sfsA"
                     /locus_tag="ACHUPB_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0758281.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4228..4758)
                     /gene="thpR"
                     /locus_tag="ACHUPB_001425"
     CDS             complement(4228..4758)
                     /gene="thpR"
                     /locus_tag="ACHUPB_001425"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0758282.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4832..7261
                     /gene="hrpB"
                     /locus_tag="ACHUPB_001426"
     CDS             4832..7261
                     /gene="hrpB"
                     /locus_tag="ACHUPB_001426"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0758283.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7458..9992
                     /gene="mrcB"
                     /locus_tag="ACHUPB_001427"
     CDS             7458..9992
                     /gene="mrcB"
                     /locus_tag="ACHUPB_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0758284.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10212..12401
                     /gene="fhuA"
                     /locus_tag="ACHUPB_001428"
     CDS             10212..12401
                     /gene="fhuA"
                     /locus_tag="ACHUPB_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0758285.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12452..13249
                     /gene="fhuC"
                     /locus_tag="ACHUPB_001429"
     CDS             12452..13249
                     /gene="fhuC"
                     /locus_tag="ACHUPB_001429"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0758286.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13249..14139
                     /gene="fhuD"
                     /locus_tag="ACHUPB_001430"
     CDS             13249..14139
                     /gene="fhuD"
                     /locus_tag="ACHUPB_001430"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0758287.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14136..16118
                     /gene="fhuB"
                     /locus_tag="ACHUPB_001431"
     CDS             14136..16118
                     /gene="fhuB"
                     /locus_tag="ACHUPB_001431"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0758288.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16276..17556)
                     /gene="hemL"
                     /locus_tag="ACHUPB_001432"
     CDS             complement(16276..17556)
                     /gene="hemL"
                     /locus_tag="ACHUPB_001432"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0758289.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17722..17769
                     /gene="yadX"
                     /locus_tag="ACHUPB_001433"
     CDS             17722..17769
                     /gene="yadX"
                     /locus_tag="ACHUPB_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0758290.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17781..19202
                     /gene="clcA"
                     /locus_tag="ACHUPB_001434"
     CDS             17781..19202
                     /gene="clcA"
                     /locus_tag="ACHUPB_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0758291.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19226..19291
                     /gene="yadW"
                     /locus_tag="ACHUPB_001435"
     CDS             19226..19291
                     /gene="yadW"
                     /locus_tag="ACHUPB_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0758292.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19284..19628
                     /gene="erpA"
                     /locus_tag="ACHUPB_001436"
     CDS             19284..19628
                     /gene="erpA"
                     /locus_tag="ACHUPB_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0758293.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19675..20298)
                     /locus_tag="ACHUPB_001437"
     CDS             complement(19675..20298)
                     /locus_tag="ACHUPB_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0758294.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20336..21136)
                     /gene="btuF"
                     /locus_tag="ACHUPB_001438"
     CDS             complement(20336..21136)
                     /gene="btuF"
                     /locus_tag="ACHUPB_001438"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0758295.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21129..21827)
                     /gene="mtnN"
                     /locus_tag="ACHUPB_001439"
     CDS             complement(21129..21827)
                     /gene="mtnN"
                     /locus_tag="ACHUPB_001439"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0758296.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21911..>22741
                     /gene="dgt"
                     /locus_tag="ACHUPB_001440"
     CDS             21911..>22741
                     /gene="dgt"
                     /locus_tag="ACHUPB_001440"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0758297.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         5144 a         5796 c         6358 g         5443 t
ORIGIN      
        1 aaatgctctt gtatgtaaga cttcacgcaa cgtctcccca tcaggaaacg ccagctccgg
       61 tgcaatctca aacagcggcc acagcataaa tccacgattc ttcatatcgt agtgcggaac
      121 ggtcaggcgt tcggtattta tcacttcatt accaaacagc atgatatcga gatccagcgt
      181 gcgtggtccc cagcgttcag ctttacgaac acgaccctgt tgcagttcta tgcgctgtgt
      241 atggttgagt aactcttccg gggcgagcga agtttcaagc gccacggcgg cgttgaggta
      301 atcgggttga tcttgcggcc ccagcggtgg agtgcggtaa aacgaagaaa cggcaagaat
      361 gtggctttca gggatatcgc ctaatgcttt cagggcagca ttgacctgct ccagcggaga
      421 ggccagattg ctgcctatgg caatatacgc cactgtcatg cggtaccctc acgacgcggt
      481 gcgcgtttgc gtggacgacg agtacggcga cgcggtgacg gctcttcatc caactcgtta
      541 agcatccctt tttgatctgg tggcgcggaa acctggaact caccccacca tttcaccaga
      601 cgctgcagtt cagcgttacg ttcaacttca gctcgcaagg ccaacaggtc ataagccgca
      661 cggaacttag gatgctccag cagtttccat gcgcgtttac cctgacgtcg ggacatacgc
      721 aactgcaact gccagatatc gcgggttaac gtcgtcaggc gtttcgggat tgccagtgaa
      781 cggcaggctt cgtccagcac gtcgttcatc gccagcgcga aagcgtcgtg ataggtcagg
      841 ccgctttcct gggcgatctt ctgtgccgtc tccagcagtg ggtaccagaa catggcggca
      901 aacaagaacg ccgggttcac gcgcatatcg ttatggatac gcgtatcggt attcttcagc
      961 acctgttcaa tgatccgctc catcgggctg tcgccatttt ccgtgaagta gcgggtaatg
     1021 gtcgggaaca gcggctggaa cagatgatat tcacacaaca gcttataggt ttcgtaaccg
     1081 tagcccgctt gtagcagttt aagcgattct tcaaacaggc gtgccggtgg gatatcgttc
     1141 agcagggtag cgaggcgagg gatcggttct gcggtttccg ggctgatgcg catacccaat
     1201 ttggcggcaa aacgtaccgc gcgcagcatc cgtaccggat cttcacggta gcgcgtttcc
     1261 gggttaccaa tcagacggat aacgccgtcc ttcagatcct tcataccgcc aacgtaatca
     1321 cggacggtaa aatccgctac gctgtaatag aggctgttga tagtgaaatc gcggcgctgg
     1381 gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca acatgccgtt ttgcccgcgt
     1441 tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc cacggaaggt cgcaacttcg
     1501 ataatctccg ggccaaacat tacatgagcc agacggaaac ggcgacccac caggcggcag
     1561 ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag tggttacgtc aaaatctttc
     1621 ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa ccagccaggc ttcgtatccc
     1681 gctttgttga gcctgtacat taccttcagg gcattttcac tgatatcttt gcgggaaata
     1741 gcatgctgct cacgcgggat caccgtcacc tgtggacggg cgactgcctg ttcagcctcg
     1801 ctttcctcgc ggcttagcac cttgcggcaa aaattagcga ctcgggtaaa aatagtacac
     1861 ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg gctaatcata gctcagcatg
     1921 acgcatttga gaatgttgaa tttacaattg ccgactcggg cacggcggtc agcgtccagt
     1981 ttttgactgc tgactgaagg atttgttcga cgctgaaatc ctgccagtgt gcttctgcct
     2041 gctgacccag aaattgaagt gccgcgatta gtaccggacg tggatcgcct ttcggcaacg
     2101 caggcgcatg attctgcttg gaaagtttag cgccttgtgg attaagcgcc agcggcagat
     2161 gaatgtaatc tggcactttc cagccaaaaa gctggtacag cgagatttgc cttactgttg
     2221 gttcaatcag atcagcccca cgcactattt ctgtaacgcc ctggaaatga tcatcaacca
     2281 caacagccag gttgtaggcg aacaacccat cacggcgatg aatgataaaa tcttcccgtg
     2341 ccagtttttc gtcggcgtga ataatgccgc gcagctggtc agtaaattgc gtgaccggat
     2401 gctgctggcg gatacgcact gcggcattgt ctggtccatg atgcaacacc cggcaatgac
     2461 cgtcgtaaat accgccaatg ctttgaatac gcgcacgcgt acaggtgcag taataactta
     2521 gtccttgttc atgtaaccag gcgagtgctt cacgataggc gtcgtgacgt tgcgattgcc
     2581 agagaacatc gccgtcccag tgcagaccgt aatgttccag ctggcgcagg atagtttctg
     2641 cggcaccggg aacttcacga ggcgggtcga tatcttctat gcgtaccagc cagcgacctt
     2701 gccgggcgcg agcctgcaaa tagctgccga gcgcggcgat cagagagcca aaatgaagct
     2761 cgccggaagg agagggggcg aagcggccaa tatactgtgt gtctgtcatc tctttgaaca
     2821 aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg atggaacggc tgtaattagc
     2881 cagccatctg tttttcgcga atttcagcca gcgttttgca gtcgatgcac agatcggctg
     2941 tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc gcaggattcg cagtagccga
     3001 aatcttcgtc ttccactttt ttcagcgtct tctcgatctt tttgatcagc ttacgctcgc
     3061 gatcgcggtt acgcagttcg aggctgaact cttcttcctg ggctgcacgg tctaccgggt
     3121 ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt gcgatcgact tcatccctga
     3181 gttgattacg ccatgcttcc agaatacgac ggaagtgcgc cagctgggct tcattcatat
     3241 actcttcgcc cggcttctcc tgatatggtt ccaccccagc gatggcgaga atactcaggg
     3301 acgatgtttt acggttttgc ccttcttgca tgttgcttct ccttaacacg cactatcgat
     3361 ccccatgttc gggggaaaaa tgaggccgct ataaatagca gatgcttttc cggatagcaa
     3421 ttatctaaac gtaacacttg acaactgtgt gaggaaaagc gtatttgcgc acgcgaccag
     3481 aatgtaaatt aaccagttac ttactttact acaatgtaac cgacagtgat tttttaagag
     3541 ccatgccttc agcagaaatt tccgctttgt aagccagaat ttctaccccc ctctgttgag
     3601 cttctgacaa tagttgcgcg tatttctcat cgatgtggcg cgcgggtgaa aaccgtgtaa
     3661 tggctgaatg cagcacggcg aaaaagataa ccgcacgctg gccttcagcc gctacgttca
     3721 tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc atcgggaaaa tatccctgtt
     3781 cgttctccgc taacgtaacc gatttcactt caatatagca gtctggacgc gaatccgcct
     3841 gcaacataaa gtcaatacgg ctgcgttctg cgccgtattt tacttcgctt ttcagcgagc
     3901 tatagcctga cagttctgaa attgattcat taaggatagc ctctttcgtc aacctgttag
     3961 cccaaagcgt attgacgcaa ataaatgcgc cgctctggct ttgagttagt tcccaggtgt
     4021 gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca gacggtatcg ccacgcgttg
     4081 cacaaccggt catcgcaccc gtattcgggc agtgtagcgt taattcgcga ccatcgggtg
     4141 tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag cgtcgcgcgc tgtagagggg
     4201 gagagaattc cattgcgaca atccttgtta ttgcgttagc gcccagcgtt ttagcggcgt
     4261 gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg gtgaactccg tcaccgcata
     4321 agaccagtta aaacctggcg gcgggattgt caccgcctcg ctggcgtcgc gcaataaggt
     4381 aatatgtgga tgaaacggac gattgctttg aaaacaaccg ctgcgggcag cctgtgaacg
     4441 gagcatattc gccagctgga ttaagccgcg tggcggctga cgcatcccta accacaccac
     4501 acgcgaacgc agccattgtc cggcgtcatc aagcgtgagt gtgaaaccag gttgacgaat
     4561 ccgtccggct aaaagagaaa gcgccttctc tttctcggcg ctcacttcgc ctaaaaatgc
     4621 cagagtcaga tgcaaattat cggcggcgac cggacgtccc gcctcaggtg ggaagtgtgt
     4681 ggcgcgccag tggataatct gttcgcggat ttctgcaggt aagtcgatag caaagaacag
     4741 acgttgcggt tcagacatgt gaggcactcg gttatgaatt accgcgatgc tacaatgtgg
     4801 cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg ttgcccgttg ctgccgtctt
     4861 acctgaatta cttaccgctc tcgattgtgc gccgcaggta ttattaagtg cgccgaccgg
     4921 ggccgggaaa tcaacctggc tgccgctgca actgctggcg catcccggca ttaacgggaa
     4981 aattatcctg ctggagccgc gtcgtctggc ggcacgtaac gtcgcgcaac ggctggcgga
     5041 gctgcttaac gaaaagccag gcgataccgt tggctaccgg atgcgtgcgc aaaactgcgt
     5101 cgggccaaat acccgtctgg aagtagttac cgaaggcgtg ctgacgcgga tgatccagcg
     5161 tgacccggag ttgagcggtg ttggattggt gatccttgat gaatttcatg agcgcagctt
     5221 gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa caaggtctgc gtgatgacct
     5281 taaactgctg attatgtcgg ctacgctgga taacgaccgc ttgcagcaaa tgctgccaga
     5341 agcgcccgtc gtcatctcag aagggcgctc gtttccggtt gaacgccatt atttaccgct
     5401 gcccgcgcat cagcgttttg acgaagccgt tgcggtagcc accgccgaaa tgctgcgtca
     5461 ggaaagcgga tcattactgt tatttttacc tggcgtcgga gaaattcagc gtgtgcagga
     5521 acaactggct tcgcgcatcg gcagtgatgt gttgctctgc ccgctgtatg gcgcgttgtc
     5581 gctgaacgat cagcgaaaag cgatcctccc ggcaccgcaa gggatgcgca aagtggtgct
     5641 ggcgaccaat attgctgaaa ccagtttaac cattgaaggt attcgtctgg tggtggattg
     5701 tgcccaggag cgtgtggcgc gttttgagcc gcgcacgggg cttacgcgac tgattactca
     5761 acgcgttagc caggcatcca tgacgcagcg tgccgggcgc gccgggcgtc tggagccggg
     5821 tatctgcctg catttaatcg ccaaagaaca agcagaacgc gctgccgcgc aaagtgaacc
     5881 ggagatctta caaagcgatc tttccggttt gctgatggaa ttactgcaat ggggatgcag
     5941 cgatccggcg cagatgagct ggctggatca accgccagta gtgaatctaa tggccgcgaa
     6001 acgtctgtta caaatgctgg gggcgctgga cggtgaacgg cttagtgcgc aagggcaaaa
     6061 aatggcagcg ctgggtaacg atccgcgttt agcggcaatg ctggttagcg cgaagaacga
     6121 cgacgaagct gctaccgcgg caaaaattgc cgccattctc gaagagccgc cactgatggg
     6181 caatagtgac ctgggcgtgg cgttttcgcg caatcaacca gcctggcagc aacgtagtca
     6241 gcaactgtta aaacgcttaa acgtacgcgg cggtgaggca gacagttcgc ttatcgcgcc
     6301 gctacttgcc ggggcgtttg ccgatcgcat tgctcatcgc cgtgggcaag atggacgcta
     6361 tcaactggca aacggcatgg gagcgatgct cgatgccgac gacgcgctaa gccgccacga
     6421 atggttgatc gcaccgttat tattgcaggg cagcgcctcg ccggatgcgc ggattttact
     6481 ggcgctaccg gtcgatattg atgaattagt acaacgctgt ccgcagctgg tgcagcaatc
     6541 tgacaccgtg gaatgggatg acgcgcaagg tacgctgaaa gcctggcgtc ggctacaaat
     6601 cggtcagttg acggtgaaag tgcagccgct ggcgaaaccg tcagaagacg agttgcatca
     6661 ggcgatgctt aatggcatcc gtgataaagg tttaagcgtg ctcaactgga cggcggaagc
     6721 ggaacagcta cgcttgcgtt tgttatgcgc cgcaaagtgg ttgccggaat atgactggcc
     6781 agcggttgat gatgaaagtt tattggcagc gctggaaacg tggctgctgc cacatatgac
     6841 aggcgtacat tcactacgcg gcctgaaatc actggacatt tatcaggcac tacgcggatt
     6901 acttgattgg ggaatgcagc aacgtctgga tagtgaattg cctgcgcatt acactgtgcc
     6961 gacgggaagc cggatcgcca ttcgttatca tgaagataac ccgcccgcac tggcggtgag
     7021 aatgcaggag atgtttggtg aggccactaa tccgactatc gcccaggggg gcgtgccgct
     7081 ggtgctggag ttgctttcac ccgcccaaag gccactacaa atcacacgag atttgagcgc
     7141 cttctggaaa ggagcgtacc gtgaggtgca aaaagagatg aaagggcgtt atcccaaaca
     7201 cgtctggccg gacgacccgg caaacacggc accgacgcga cggacgaaaa agtattcgta
     7261 aggttggttt tctccctctc cctgtgggag agggccgggg tgagggcatc agcgtgcgca
     7321 tttccccctc accctaaccc tctccctcaa gggagagggg accgatcgag cacaaatttt
     7381 gagagatatc ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa
     7441 tattgcggag aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac
     7501 cgacgcgtcc ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg
     7561 acgattatga tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca
     7621 aagggcgtaa gcctcgtggc aaacgcggct ggctatggct gctgttaaaa ctggctatcg
     7681 tttttgccgt gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta
     7741 ttgatggcaa ggtctggcaa ctgcccgcgg cagtttatgg ccgaatggtc aaccttgaac
     7801 cagatatgac catcagcaag aacgaaatgg tgaagctgct ggaggcaaca cagtatcgtc
     7861 aggtgtcgaa aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga
     7921 tccgccgtcc gtttgatttc ccggacagca aagaagggca ggtgcgtgcg cgtctgacct
     7981 ttgatggcga tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggtttct
     8041 tccgtcttga tccgcgcctg atcaccatga tctcttcgcc aaacggtgag cagcgtctgt
     8101 ttgtgccgcg cagtggtttc ccggacttgc tggtggatac tttgctggcg acagaagacc
     8161 gtcatttcta cgagcatgat gggatcagtc tctactccat cggacgtgcg gtgctggcaa
     8221 acctgaccgc cggacgcacg gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa
     8281 acctgttcct ctccagcgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc
     8341 tgatcatgga cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt
     8401 atctcggtca gagcggcgac aacgaaatcc gcggcttccc gcttgcgagc ttgtattact
     8461 ttggtcgccc ggtagaagag ctcagcctcg accagcaggc gctgttagtc ggtatggtga
     8521 aaggggcgtc catctacaac ccgtggcgta acccgaaact ggcgctggag cgacgtaatc
     8581 tggtgctgcg tttgctacaa caacagcaga ttatcgatgg tgaactgtat gaaatgctca
     8641 gcgcgcgtcc tctgggcgta cagccgcgcg gtggggtaat ctctccacag ccagccttta
     8701 tgcaactggt gcgtcaggag ctgcaggcga aattaggcga taaggtaaaa gatctctccg
     8761 gcgtgaagat cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg
     8821 tggaaggcat tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcaattg
     8881 tggtcgtcga ccgctttagt ggtgaagttc gtgcgatggt gggaggttct gagccgcagt
     8941 ttgcgggcta caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag
     9001 cgacttatct gacggcttta agccagccga aaatctatcg cctgaacacg tggattgccg
     9061 atgcgccaat cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aacgatgacc
     9121 gtcgttatag cgaaagcggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg
     9181 tgccgacggt aaatctgggg atggcgctgg ggctgcctgc agttacggag acctggatca
     9241 aactgggcgt gccgaaagat cagctgcatc cggttccggc gatgctgctg ggggcgttga
     9301 acttaacgcc aatcgaagtg gcgcaggcat tccagaccat cgccagcggc ggtaaccgtg
     9361 caccgctttc tgcactgcgt tcggtaatcg cggaagatgg caaggtgctg tatcagagct
     9421 tcccgcaggc agaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc
     9481 agcaggtggt acaacgtggt acgggtcgtc aacttggggc gaaatacccg aacctgcatc
     9541 tggcagggaa aacagggact accaacaata acgtagatac ctggtttgcg ggcattgacg
     9601 gcagcacggt gaccatcacc tgggtaggcc gtgataacaa ccagccgacc aaactgtatg
     9661 gtgccagcgg ggcaatgtct atttatcagc gttatctggc taaccagacg ccaacgccgc
     9721 tgaatcttgt tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg
     9781 tttgcagcgg tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc
     9841 agcagagcga gatgcagcag cagccgtcag gcaatccgtt tgatcagtct tctcagccgc
     9901 agcaacagcc gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg
     9961 gttggatcaa ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga
    10021 attgccatcc ggtaaaataa catcccacct aaagtattaa ccctttcttt tcatctggtt
    10081 gtttattaac ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc
    10141 gaatattatc ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca
    10201 gagatatacc aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg
    10261 cagttgtagt agccacagcg gttagcggca tgtctgttta tgcacaggca gcggttgaac
    10321 cgaaagaaga cactatcacc gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc
    10381 ctgctgcaac tattgcggcg aagcactctg ctactgcgac taaaacggat acaccaattg
    10441 aaaaaacgcc gcagtctatt tcggttgtga caaatgaaga gatgcagatg cgtcaatttc
    10501 agtctgtaaa agaagcatta ggttatacac ctggcgttac ggttagcagt cgtggtgctt
    10561 ctaatacata tgattttgtt atcattcgtg gtttctcatc tgttggtctg aatcaaaata
    10621 attaccttga tggactaaaa cttcagggca acttttataa cgatgctgtg attgatcctt
    10681 acatgctcga gagggttgaa ctgatgcgtg ggccgacgtc tgttctctat ggaaaaagta
    10741 atcctggtgg gattatttct atggtgagta agcgtccgac cactgaaccc ctgaaagaaa
    10801 ttcagtttaa aatggggacg gataatctgt ttcagaccgg attcgatttc agtgatgcac
    10861 tggatgataa cggtgagttc tcttaccgtt tgacgggcct cgctcgctca acaaacgaac
    10921 agcagaaaaa ctctgaatct cagcgttata ccattgctcc atcattctca tggcgtccag
    10981 acgacaaaac caattttact ttcctgtcct atttccagaa tgaaccagaa acgggttatt
    11041 acggttggtt gccgaaagag gggaccgttg agccattgcc taatggtaag cgtctaccga
    11101 ctgacttcaa tgaaggtgca tcgaataata catactcccg taaccagaaa atggtgggat
    11161 atagttttga acatggtttt aatgacacct tcaccgtgcg tcagaatctg cgtttcagtg
    11221 aaatgaaaac ctcacagaaa agtgtttatg gcacagggat tgctggtgat ggtcataccc
    11281 taaaccgcgg gacagtggtg gataatgagc gtctacaaaa ctttagcgtt gatacccaac
    11341 ttgaaagtaa gtttgctaca ggtgacgtcg agcatacttt gctgacaggg gtagacttca
    11401 tgcggatgcg taatgatatc aatgccagct ttggatccgc accatccatc gatctttata
    11461 acaaatatca ccctgaatac tttgcatttg gtagcgcaga gccataccag atgaatgaaa
    11521 gcaaacaaac aggtatttat gttcaggatc aggcggaatg gaataaatgg gtattcactc
    11581 tggggggacg ctacgattgg tctaagcaag cgaccactgt tcgtgaaaac tcttatacgc
    11641 cgactgaagg ttatattgag cgcaatgatc atcagttcac ctggcgcggt ggtgtaaatt
    11701 acttattcga taatggtatt tcaccttact ttagctatag ccagtccttt gaaccgagtg
    11761 ctttcgatct gtggagcaac ccgcgcattt cctataagcc atcgaaaggt gaacagtatg
    11821 aagctggcgt aaaatatgtt ccgaatgata tgccggtcgt tgttacgggc gcagtctatc
    11881 aattgacgaa aacaaataac ctgacagcag acccaacaaa cccattagcg caagtcccag
    11941 caggtgagat tcgcgctcgt ggtgtggaac ttgaagcaaa agcggcgtta aatgccaata
    12001 ttaacttgac ggcttcttat acctacaccg atgcggaata caccaaagac accaatctca
    12061 aaggtaaaac tccagaacaa gtaccggagc atatggcatc tctctggggg gattatacct
    12121 tcaatgaagg gccgctttct ggtttaacat tgggaacagg tggtcgtttt attggttcca
    12181 gctatggtga tccggcaaac acttttaaag tgggtagcac agctgtaatg gatgctgttg
    12241 taaaatatga tctggcacgc tttggtatgg cgggatccag ccttgctgtg aacgtcaaca
    12301 atttgctcga tcgtgagtat gttgccagtt gcttccagac ttatggctgc ttctggggcg
    12361 cagaacgtca ggtcgttgca accgcaacct tccgtttcta atttctcttt tgggacacgg
    12421 atttccgtgc ccatttcaca agttggctgt tatgcaggaa tacacgaatc attccgatac
    12481 cacttttgca ctgcgtaata tctcctttcg tgtgcccggg cgcacgcttt tgcatccgct
    12541 gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt ggtcacaacg gttctggtaa
    12601 atccactctg ctcaaaatgc ttggtcgtca tcagcctccg tcagaagggg agattcttct
    12661 tgatgcccag ccgctggaaa gctggagcag caaagcgttt gcccgcaaag tggcttattt
    12721 gccgcagcag cttcctccgg cagaagggat gaccgtgcgt gaactggtgg cgattggtcg
    12781 ttacccgtgg catggcgcgc tggggcgctt tggtgcggca gatcgcgaaa aggtcgagga
    12841 agctatctcg ctggttggct taaaaccgct ggcgcatcgg ctggtcgata gtctctctgg
    12901 cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg caggatagcc gttgtctgtt
    12961 gctcgacgaa ccgacttcgg cgctggatat cgcccaccag gttgatgtgc tggcgctggt
    13021 gcaccgttta agtcaggaac gtggcctgac ggtcattgcc gtgttgcacg atatcaatat
    13081 ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt ggtgaaatga ttgctcaggg
    13141 aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg atttatggca tcccgatggg
    13201 tattttgccg catccggcgg gtgctgcacc tgtgagtttt gtttattgat gagcggctta
    13261 cctcttattt cgcgccgtcg actgttaacg gcgatggcgc tttctccgtt gttatggcag
    13321 atgaataccg cccacgcggc ggctattgac cccaatcgta ttgtggcgct ggagtggttg
    13381 ccggtggaat tactgctggc gctcggcatc gtgccttacg gcgtggcgga taccatcaac
    13441 tatcgcctgt gggtcagcga accaccattg ccggactcag tgattgacgt cggtttgcgt
    13501 acagaaccta accttgaact gctgaccgaa atgaaaccgt cgtttatggt ctggtcggca
    13561 ggatatggcc cttcatcaga aatgctggca cgtattgcgc cgggtcgcgg atttaacttc
    13621 agtgacggca aacagccgtt ggcgatggcg cgtaaatcgc tgacggaaat ggcagattta
    13681 cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat acgaagactt tatccgcagc
    13741 atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac tgctgacgac gcttatcgat
    13801 ccgcgccata tgctggtctt cggtccaaac agcttgttcc aggaaattct cgatgagtac
    13861 ggcatcccaa atgcctggca aggggaaacc aacttctggg gcagtaccgc cgtcagtatc
    13921 gatcgtctgg cagcgtataa agacgttgat gtgctctgtt ttgatcacga caacagcaaa
    13981 gacatggatg cgctaatggc aaccccgctg tggcaggcca tgccgtttgt ccgcgccgga
    14041 cgctttcagc gcgtacctgc agtctggttt tatggtgcga cactctcggc aatgcacttt
    14101 gtgcgcgttc tggataacgc catcggaggt aaagcgtgag taaacgaatt gcgctttttc
    14161 cggcgttatt gctggcgctg ttagtgattg tcgctacggc gctcacctgg atgaacttct
    14221 cgcaggcgct gccgcgtagc cagtgggcgc aggctgcctg gtcgccagac atcgatgtca
    14281 tcgagcagat gatttttcac tacagcttgt tgccgcgtct ggcgatttcg ctgctggtgg
    14341 gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaac ccgctggcgg
    14401 agccgacgac gcttggcgtt gctacaggcg cgcaactggg aattaccgtc accacacttt
    14461 gggcgatccc aggtgctatg gcgagccagt ttgccgcact ggtgggcgct tgtgtcgttg
    14521 gcttaatcgt ctttggcgtc gcgtggggga aacggctttc gccggttacg cttattctcg
    14581 cggggctggt ggtgagcctt tattgcgggg caattaatca gctactggtt atcttccatc
    14641 acgaccagct gcaaagcatg ttcctgtgga gcaccggaac gctgacgcaa accgactggg
    14701 gcggcgttga gcgtttatgg ccgcagttgc tgggcggcgt gatgttgacg ttattgctac
    14761 ttcgcccgtt aaccctgatg gggcttgatg atggcgtggc gcgcaatctc gggctggcct
    14821 tgtcgctcgc gcgtctggcg gcgctgtcgc tggcgatagt catcagtgcg ctgctggtga
    14881 atgcggtggg gattatcggc tttatcggtt tgttcgcgcc gctgctggcg aaaatgctgg
    14941 gggcgcggcg tttgttgcca cggttgatgc tggcgtcgct gattggggcg ctgatcctct
    15001 ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg gatggaagtg tccaccggtt
    15061 cggtcactgc gttgatcggt gcgccgctgc tactgtggct gttgccgcgt ttacgcagca
    15121 ttagcgcgcc ggatatgaag gtcaacgatc gtgtcgcggc tgaacgccaa catgtgctgg
    15181 cgtttgccct cgcgggcggc gtgctgctgt tgatggctgt ggtggtggcg ctgtcgtttg
    15241 gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt gctcgaggat ttgatgccct
    15301 ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt catgctggcg gtggcgggct
    15361 gtattattca gcggctgacc ggaaacccga tggcaagccc ggaagtgctg gggattagct
    15421 ctggcgcggc gtttggcgtg gtgttaatgc tgtttctggt gccgggtaat gcctttggct
    15481 ggctgttacc cgcgggcagt atcggggcgg cggtgacgct gttgatcatt atgatcgccg
    15541 ccggccgcgg tggattttcc ccacaccgta tgttactggc ggggatggcg ttaagtaccg
    15601 cattcaccat gcttttgatg atgttgcagg cgagcggtga cccgcgaatg gcgcaagtgc
    15661 tgacctggat ttccggttcg acctacaacg cgaccgatgc gcaggtctgg cgtaccggaa
    15721 ttgtgatggt gattttgctg gcgattaccc cgctgtaccg ccgctggctg accattttac
    15781 cgctgggtgg tgataccgca cgagccgtag gaatggcgct gacgccgacg cgaattgcgc
    15841 tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat gactattgga ccgttgagtt
    15901 ttgttggttt aatggcaccg catattgcgc ggatgatggg ctttcgacgg acgatgccac
    15961 acatcgtaat ttcggcgctg gtgggtggtt tactgctggt attcgctgac tggtgtgggc
    16021 ggatggtgct gtttccattc cagatcccgg cggggctgct gtcgaccttt atcggcgcgc
    16081 catattttat ctatttgctg agaaagcaga gccgttaatt tttccatata acggccctgt
    16141 acgcctggac ggataaagcg tttacgcgac aacggatgcc cgatgcgacg ctggcgcgtc
    16201 ttatcgggcc tacaaacggc cccgaatcgt aggtcggata aggcgttcac gccgcatccg
    16261 acaaaccatg ctggatcaca acttcgcaaa cacccgacgt gcagcatcga tggtgttatt
    16321 gatatcttcc atgctgtgcg ccacggacat aaagcccgct tcaaacgctg acggtgccag
    16381 gtaaacacct tcgtccagca tcatatggaa gaaacgctta aagcgttcca cgtcacaggc
    16441 catcacatcc tgatagcacg tcacggactc ggcgtcggta aagaaaatac cgaacatgcc
    16501 gccaacgtgg ttaacgacca gcggaattcc ggcttcttct gccgcttcca gcagaccttc
    16561 tgccagacgt gttgtcagct catccaacgt ttcgtgaacg cccggttgcg cgacttcatt
    16621 cagacaggcg aaaccggctg ccatcgcaat tgggttaccg gaaagcgtac ccgcctgata
    16681 gaccggaccc gtcggggcca gcgcatccat tacatcacga cgaccaccga atgcgcctac
    16741 cggcattcca ccgccgatga ttttgcccag gcaggtgaga tccggttcca caccgtaata
    16801 atcctgtgcg ccagccagtg ccacgcggaa gccggtcatt acttcatcga tgatcagcaa
    16861 tgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg aagtctggca gcggtggaac
    16921 acagttcata ttgcctgcca ccggctcgat gataatacag gcaatctctt gcgggtattg
    16981 ctcaaacgcg gcgcgtacag aagccagatc gttataagta caggttaagg tatgtttggc
    17041 gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc
    17101 tttcaccagc aggcagtcag cgtgaccgtg gtaacaccct tcaaatttaa tgattttgtc
    17161 gcgaccggta aaaccacggg ccagacggat ggcgctcatc gtcgcctcgg tgccggagtt
    17221 caccatgcgc accatatcca tggtcgggac caattcggtc accagttgcg ccattttcac
    17281 ttccatttcg gttggtgcac caaagcttaa accacgctcg gcggcttcaa tcacggcatt
    17341 gcggattgcc ggatggttat ggcccagcac catcggcccc caggaaccga cataatcgat
    17401 ataggctttg ccatcaacat cgtacagata agcgccgtcc gctttttcga taaacagtgg
    17461 agtgccgccc acgccagtaa aggcgcgaac aggggagttc acaccgccag ggatcagctc
    17521 gcgcgctgcg ctgtaaagat tttcagactt actcatggag ggttcctgat tcgtagaaaa
    17581 agtgaatggc tgctattcta tgttattcat aacaagttaa atactcgtca aacatcaggc
    17641 tgcttgtact ggtgcaatcc tgatttctta gagtataaaa gttttgtgca tttgaaacat
    17701 tacgctttgc aaaggatttt catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat
    17761 gaccaataag tgatcattgg atgaaaactg atactccctc tttagaaaca ccgcaggccg
    17821 cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga gcgcgataaa accccgttag
    17881 ccattttgtt tatggcggca gtcgtcggca cgcttgttgg gctggcagcg gttgcttttg
    17941 acaaaggtgt cgcctggttg cagaaccaac gtatgggggc gctggtacat actgctgata
    18001 attatccgct tctgttaacc gtcgcttttc tctgttcggc ggtgctggcg atgtttggct
    18061 actttttggt gcgcaaatac gcgccggaag caggtggttc ggggatcccg gaaattgaag
    18121 gggcgctgga agatcaacgt cccgttcgct ggtggcgtgt attgccggtg aagttctttg
    18181 gcgggctggg gacactcggc ggaggcatgg tgttggggcg cgaagggcca accgtgcaga
    18241 tcggcggtaa cattggccgt atggtgcttg atattttccg cctgaaaggt gacgaagctc
    18301 gccatacgct gctggcaacc ggtgctgctg cggggctggc tgcggccttt aacgcgccgc
    18361 tggcgggtat tttgtttatt atcgaagaga tgcgtccgca gtttcgctat acgttaattt
    18421 cgataaaagc ggtatttatt ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc
    18481 atgaagttgc gttgattgac gtcggtaaac tttctgatgc gccgcttaat acgctgtggc
    18541 tttatctgat cctcggtatt atttttggca ttttcggccc tatttttaat aaatgggtgc
    18601 tggggatgca ggatttgctg caccgtgtgc acggcggcaa tattaccaaa tgggtactga
    18661 tgggcggtgc gattggcggt ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg
    18721 gcggcggttt taacctgatt cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg
    18781 tgtttatctt cgtcgctcgg gtcattacca ccttactctg cttctcttcc ggcgcgccgg
    18841 gcggtatttt tgccccgatg ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg
    18901 ttgccgttga gctgtttccg caatatcacc ttgaggcggg gacgtttgct attgccggaa
    18961 tgggggcatt actggcggca tctattcgcg cgccgttaac ggggatcatt ctggttctgg
    19021 agatgaccga taactaccag ctcattttgc caatgattat taccggtctt ggcgcaacac
    19081 tattagcgca atttaccggc gggaaaccgc tatactcggc gattcttgcg cgcacgctgg
    19141 caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc atcagccagc gagaatactt
    19201 gaacgaaata ccagggtatt agataatggc gattattatt gggttagaat ttgcccaatt
    19261 gccgatgtcg tttggagcaa aatatgagtg atgacgtagc actgccgctg gagtttaccg
    19321 acgcagcagc caacaaagtt aaaagcctga tcgctgacga agataacccg aatctgaaat
    19381 tacgcgtgta tatcaccggt ggcggttgca gcggcttcca gtatggtttc acctttgatg
    19441 atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg cgttggcttg gtggttgatc
    19501 cgatgagcct gcaatatctg gtcggcggtt ccgttgacta taccgaagga ctggaaggtt
    19561 ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg cggttgcggt tcttccttta
    19621 gcatctaatc tgttgttcat gtgccggata aactatccgg cgcatcttct ctcttcaacg
    19681 cccattctca tccagcgcaa acgtcggtag cttaagatgc caacgaatag ccgccagccg
    19741 aatcaatagc gtcacgacca tgcccatcat actggctgtt tccagtggta cggaaaatgt
    19801 gtaataagcc gtagcgtgga caataccgcc gataatacag gcagttgcgt agatttctgt
    19861 acgtaaaatc atggggatct cgcgggccag tacatcacga atgatcccgc cgccaacgcc
    19921 agtaatgacg accatacaaa ccgcgattaa cggaccgact tccgcattaa aggctttatt
    19981 cacgccaatg ccgacaaaca ccgccagacc aacggcgtcc agcaccggca acatccattt
    20041 tggtaagcgt cttggctggc gcaccagcac gatggtcagc atgctggtta ccattgcaac
    20101 gaccagatcg gtgggatctt tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg
    20161 aattgtcccg ccgcctacag cggtaaccac gcccagtacc agaacaccaa aagggtccat
    20221 acgcaatttt ccggctaaca aaacgccgga gatggcaaat accgctgtgc cgactatatc
    20281 cagccaatag acgagcattg ttcaatcccc actgagcacc taaaaaggtc aggcgctaat
    20341 ctacctgtga aagcgcatta cagagctgtt gtgcagcgag gataatacgt gggcttgcac
    20401 gttcaaacca gtcactcgtg agaggaataa cgggaatttt gagctgttca ccccagtatt
    20461 gtttgatttt aggaatttgg tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg
    20521 ctaacacctg ttcgcggcta acttgcggcc agggaacccg gctgtcttta aagatgtttt
    20581 ctccgccaca aacttcgagt acctggtttt gaatcgactc ttttccactg gtaaatggcg
    20641 gattaatgcc gaattgcaga aaaacacgtt ttttaggttt atcagcatat tgcgctttca
    20701 attgcgcgta ctgatccagc agggattgcg cggcttgttc ggccttatcc ggttgcggac
    20761 tccagggggc caattgacgt aacgcattgg caatttgttc aatgcttgtc gcatcgaccc
    20821 acatcacttt tattcccagc gaagccagct ggtcaacctg ccgctcggca ttacctccac
    20881 gccaggcaat caccagatcg ggtttcagcg cgacaatgcg ttccagattc atcccctgcc
    20941 aggtggaaac ctgctcaatc ttttgcgctt gtggaggata gtcggaatag ctgctgaccc
    21001 caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga
    21061 cgcgcggcgc ggcgttgagc cacagtggcg caagaaaaga cagggcgacc agcgccctga
    21121 acagtgactt agccatgtgc cagtttctgc accagtgact caaccatcag gctggactgt
    21181 ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag actgttgatc ggccacgtcg
    21241 gagatggcgc gtacgacaac aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc
    21301 gtcgcttcca tctctacagc aatggcctgt gggaagttgt ggcggatttt cgccagacca
    21361 acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc cacgtacagc gttaagattc
    21421 agttcggcaa tgcaggcctc agcggcagcg atcagtttat cgtcagcttt aaagcctgcc
    21481 ggacagcctg gtaactgacc gtattcataa ccaaatgccg tgacatccgc gtcgtgataa
    21541 cgcgcttcgt ccgagacaac gatatcgccc actttcagcg ttggtgccag gccaccggca
    21601 gaaccggtgt taataatcac atctggcttg cagtgttcca acagcagagt ggcacccagc
    21661 gccgcagcga ctttaccgat gcccgatttc agaagcgcaa cctcggttcc attcagttgg
    21721 ccggtataga tttcgcaacc gccgagactg atagtttgac ggttttcgat tttgtcacgc
    21781 agcagcgtaa cttcttcttc cattgcacca atgatgccga ttttcataga tttactcgcg
    21841 ataagcccga tttgaaagca tagtttacca tgcgcttacg gggaagcgta tttctcacgc
    21901 gggagaggac atggcacaga ttgatttccg aaaaaaaata aactggcatc gtcgttaccg
    21961 ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg
    22021 gcgtatcatc aactctccgg caattcgtcg tctgcaacaa aagacccagg tttttccact
    22081 ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg atggaagtcc agcaggtggg
    22141 acgctacatc gccaaagaaa ttttaagccg tctgaaagag cttaaattgc tggaagcata
    22201 cggcctggat gaactgaccg gcccctttga aagcattgtt gagatgtcat gcctgatgca
    22261 cgatatcggc aatccgccgt ttggtcattt tggcgaagca gcgataaatg actggtttcg
    22321 ccaacgtttg cacccggaag atgccgaaag ccagcctctg actgacgatc gctgcagcgt
    22381 ggcggcacta cgtttacggg acggggaaga acagcttaac gagctgcggc gcaagattcg
    22441 tcaggactta tgtcattttg aggggaatgc acaaggcatt cgtctggtgc atacattgat
    22501 gcggatgaat ctcacttggg cacaggttgg cggtatttta aaatataccc gtccggcgtg
    22561 gtggcgtggc gaaacgcctg agacacatca ctatttaatg aaaaagccgg gttattatct
    22621 ttctgaagaa gcctatattg cccggttgcg taaagaactt aatttggcgc tttacagtcg
    22681 ttttccatta acgtggatta tggaagctgc cgacgacatc tcctattgtg tggcagacct
    22741 t
//
LOCUS       DAZOZD010000046        22604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOZD010000046 DAZOZD010000000
VERSION     DAZOZD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.046"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            18..719
                     /locus_tag="ACHUPB_001441"
     CDS             18..719
                     /locus_tag="ACHUPB_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0758298.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            744..3343
                     /locus_tag="ACHUPB_001442"
                     /pseudo
     CDS             744..3343
                     /locus_tag="ACHUPB_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3334..4404
                     /locus_tag="ACHUPB_001443"
     CDS             3334..4404
                     /locus_tag="ACHUPB_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0758299.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4416..4958
                     /locus_tag="ACHUPB_001444"
     CDS             4416..4958
                     /locus_tag="ACHUPB_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0758300.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4966..5481
                     /locus_tag="ACHUPB_001445"
     CDS             4966..5481
                     /locus_tag="ACHUPB_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0758301.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5447..6183
                     /locus_tag="ACHUPB_001446"
                     /pseudo
     CDS             5447..6183
                     /locus_tag="ACHUPB_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6294..7304
                     /gene="pyrD"
                     /locus_tag="ACHUPB_001447"
     CDS             6294..7304
                     /gene="pyrD"
                     /locus_tag="ACHUPB_001447"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0758302.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7478..8020
                     /gene="zapC"
                     /locus_tag="ACHUPB_001448"
     CDS             7478..8020
                     /gene="zapC"
                     /locus_tag="ACHUPB_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0758303.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8017..9126)
                     /gene="ycbX"
                     /locus_tag="ACHUPB_001449"
     CDS             complement(8017..9126)
                     /gene="ycbX"
                     /locus_tag="ACHUPB_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0758304.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9370..11478
                     /gene="rlmKL"
                     /locus_tag="ACHUPB_001450"
     CDS             9370..11478
                     /gene="rlmKL"
                     /locus_tag="ACHUPB_001450"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0758305.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11490..13397
                     /locus_tag="ACHUPB_001451"
     CDS             11490..13397
                     /locus_tag="ACHUPB_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0758306.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISHLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13527..14780
                     /gene="pqiA"
                     /locus_tag="ACHUPB_001452"
     CDS             13527..14780
                     /gene="pqiA"
                     /locus_tag="ACHUPB_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0758307.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14785..16425
                     /gene="pqiB"
                     /locus_tag="ACHUPB_001453"
     CDS             14785..16425
                     /gene="pqiB"
                     /locus_tag="ACHUPB_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0758308.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16422..16985
                     /gene="pqiC"
                     /locus_tag="ACHUPB_001454"
     CDS             16422..16985
                     /gene="pqiC"
                     /locus_tag="ACHUPB_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0758309.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17241..17408
                     /gene="rmf"
                     /locus_tag="ACHUPB_001455"
     CDS             17241..17408
                     /gene="rmf"
                     /locus_tag="ACHUPB_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0758310.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17478..17996)
                     /gene="fabA"
                     /locus_tag="ACHUPB_001456"
     CDS             complement(17478..17996)
                     /gene="fabA"
                     /locus_tag="ACHUPB_001456"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0758311.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18065..19825)
                     /locus_tag="ACHUPB_001457"
     CDS             complement(18065..19825)
                     /locus_tag="ACHUPB_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0758312.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20011..20463
                     /gene="matP"
                     /locus_tag="ACHUPB_001458"
     CDS             20011..20463
                     /gene="matP"
                     /locus_tag="ACHUPB_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0758313.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20539..21579)
                     /gene="ompA"
                     /locus_tag="ACHUPB_001459"
     CDS             complement(20539..21579)
                     /gene="ompA"
                     /locus_tag="ACHUPB_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0758314.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21934..22443)
                     /gene="sulA"
                     /locus_tag="ACHUPB_001460"
     CDS             complement(21934..22443)
                     /gene="sulA"
                     /locus_tag="ACHUPB_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0758315.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
BASE COUNT         5780 a         5328 c         5930 g         5566 t
ORIGIN      
        1 tttaagagtg actatttatg aaaacttgca taacaaaggg aattgtgacc gtaagtttaa
       61 cggcaatatt actcagctgc tcatccgcat gggcggcagg taaaggcggg attggacttg
      121 cagcaacacg tcttgtttat tcagaaggtg aagagcaaat ttcactgggt gtacgtaaca
      181 ccagtccgga tgttccttat cttattcagt catgggtgat gaccccagat aataaaaaat
      241 cagcagattt tattattaca ccaccgctat ttgtgctgaa tccggctaat gagaatctgt
      301 tacgcattat gtacattggt gcgccgttgg cgaaagacag agaaaccctt ttcttcacta
      361 gcgtacgggc agtcccttca acaacgaagc gggaagaggg aaataccctg aagattgcca
      421 cacaaagcgt catcaaactt ttctggcgac caaaaggttt agcgtatccc ttaggcgagg
      481 ctccggcgaa actgcgttgc acttcgtcag ctgacatggt tacggtcagt aacccaacac
      541 cttatttcat taccctgaca gacctgaaaa tagggggaaa ggtagttaaa aatcaaatga
      601 tttccccctt tgataaatac caattctctc tgccaaaggg ggccaaaaat agcagcgtaa
      661 cgtatcgaac catcaatgat tacggggcgg aaacgccaca actcaactgt aaatcgtaag
      721 ccgtcttcag ttaagagagc gagatgtata gaactcatcg acaacacagc ctgttaagct
      781 ctggtggagt gccatcgttt attggtgggc tggtggtgtt tgtgtcggca gcgttcaatg
      841 cacaagctga aacctggttc gatcctgctt ttttcaaaga tgatccctca atggtggccg
      901 atttgtctcg tttcgaaaaa ggacaaaaaa taacgccagg ggtttatcgt gtcgatattg
      961 ttctgaatca gacaattgta gatacgcgca acgtcaattt tgttgagata acgccagaga
     1021 aggggattgc cgcctgtttg acgactgaaa gcctggatgc aatgggtgtt aatactgatg
     1081 cgtttccggc ttttaaacaa ctggacaaac aagcgtgtgt gccattggcg gagattattc
     1141 cggatgccag cgtaacgttt aatgtgaata aactccgtct ggaaatttca gtaccgcaaa
     1201 tcgccatcaa aagtaatgcc cgtggttatg tcccccctga acgttgggat gaagggatca
     1261 acgcgctatt actgggatat tcgtttagcg gggctaacag tattcatagc agcgcagaca
     1321 gtgattctgg cgacagctat tttctgaatt taaacagtgg cgttaattta ggcccatgga
     1381 gattgcgcaa caattcaaca tggagtcgca gtagtggcca aaccgcagaa tggaagaatc
     1441 tcagcagcta tttgcagcgg gcggttattc cactaaaagg cgaactgacc gtaggtgatg
     1501 attatactgc aggcgatttt ttcgatagtg tcagctttcg tggtgtgcag ctggcgtcag
     1561 atgacaacat gctgccagac agcctgaaag ggtttgcgcc tgtggtgcgt ggtatcgcca
     1621 aaagcaatgc ccagataacg attaagcaaa atggttacac catttaccaa acttatgtat
     1681 cgcctggtgc ttttgaaatt agtgatctct attccacgtc gtcgagcggt gatttgttag
     1741 ttgaaatcaa agaagcggac ggcagcgtca atagctacag cgtaccgttt tccagcgtgc
     1801 cattactcca gcgtcagggg cgaatcaaat acgcggtgac actggcgaaa tacagaacca
     1861 atagtaatga acagcaggag agcaaatttg cctaggccac gttgcagtgg ggcggaccgt
     1921 ggggaacgac atggtatggt ggtggacaat atgctgaata ttaccgtgcc gccatgtttg
     1981 gtctgggatt taaccttggc gatttcggag caatttcgtt cgatgcgacc caggcgaaga
     2041 gtacgctggc agaccaaagc gaacataaag gtcagtcata tcgttttctg tatgccaaaa
     2101 cgctcaacca attgggcact aattttcaat tgatgggcta tcgctattcg acgtcgggtt
     2161 tctacaccct ttccgacacc atgtataaac atatggatgg ctacgaattt aatgacggtg
     2221 atgatgaaga tacgccgatg tggtcgcgtt attacaattt gttttacacc aaacgtggca
     2281 aactgcaggt caatatctcc cagcaattag gcgagtacgg ttcgttttat ttaagtggta
     2341 gccagcaaac ttactggcat accgatcaac aggatcggct attacagttt ggctacaaca
     2401 cgcaaattaa agatctctcg ctggggggtt cctggaacta cagtaagtcc cgtggtcaac
     2461 ctgatgctga tcaggtgttt gcactaaatt tttccctgcc gctcaatctg ttgctcccca
     2521 gaagtaatga tagctatacc aggaaaaaaa ttacgcctgg atgacctcta acaccagcat
     2581 cgataacgaa gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg
     2641 taacctgagc tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag
     2701 cgccagcatg gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga
     2761 taacggcagt caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca
     2821 gggcattacc ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc
     2881 agagaatact cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt
     2941 tgtcccctat gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa
     3001 acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt
     3061 tctggcggag ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg
     3121 tataccgctg cgttttggcg cgatagcgac gctggacggc gtacagacta atagcggcat
     3181 tattgatgat gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt
     3241 acgctggggc gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca
     3301 aattaactct gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc
     3361 gtgttattac tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct
     3421 gcttatagcg ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc
     3481 gttaaaaccc tggaggaaga agaaaatacg gctggagtag ttaaagacaa attttatcag
     3541 tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt
     3601 ggccgatggg cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa
     3661 attaatgact atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg
     3721 gttcctttcg aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc
     3781 ggtcaacgtt taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata
     3841 ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa
     3901 tgctacaaca tacccgcagg tgattcctgc acgactacag gtacaccggt tttagtgtat
     3961 tacctgtctg gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc
     4021 gaggtcgatc tgggcgacgc atttgcggct aactttcgtg ttgtagggca taagcctctt
     4081 ggggccagaa cggcagaact tgcaattcca gtcaggtgta acacgggaaa cgcggggtta
     4141 gttaacgtca acctgagtct gacggcaacc acagacccca gctatcccca ggcgattaag
     4201 acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc agaacaacat tatttcccct
     4261 gctggtggaa cattaccgct ctctattcct gatgatgcag acagtatcgc gcgaatgaat
     4321 gtctatccag tcagcacgac aggtgtacca ccagaaaccg ggcgatttga agccacggca
     4381 acggtgagaa taaattttga ttaacgcgtg aacgtatgaa gaaagaaacg atatttcagt
     4441 gcgttatctt gttctttagc attcttaaca tccatgtcgg gatggctggg cctgaacaag
     4501 ttagtatgca tatttatggg aatgtggtcg atcagggctg tgatgtcgcc accaaaagtg
     4561 cattacaaaa tattcatatt ggtgatttta atatcagtga ttttcaggcc gcgaataccg
     4621 taagcactgc tgctgatttg aatatcgata tcaccggttg tgccgctagt attactggcg
     4681 cggacgtcct ttttagcggc gaggctgaca cccttacgcc gacactgctc aaactaactg
     4741 acacaggcgg aagcggtggt atggcaacgg ggattgccgt gcaaattctt gatgcgcaaa
     4801 gtcagcaaga aatcccgctc aatcaggtcc agcctcttac gcccttaaaa gccggggata
     4861 acacactcaa atatcaactt cgttataagt ccacaaaggc gggagcaacg ggcggtaatg
     4921 cgacggcggt tctctatttt gatctggttt accagtgaag gagtgatgtt gaaacggata
     4981 atctggattc tgttcttact gggattaacg gggggctgtg agctatttgc ccatgatggc
     5041 acggtcaaca ttagcggatc gtttcgccgt aatacatgcg tgctggcaca ggatagcaag
     5101 caaatcaatg tgcagttagg cgatgtctcg ctgacacgtt tttttcatgg gaattatggc
     5161 ccggagaaat ctttcatcat taatttgcag gattgcggaa cagatgtcag cacggttgac
     5221 gtcacctttt caggaacccc cgacggagtg cagagcgaaa tgctgtccat tgaaagtggt
     5281 acggatgcag ccagcgggct ggcaattgcg attctggatg atgcgaagat attgattccg
     5341 cttaaccagg cgagtaaaga ctacagccta catagcggta aggtgccatt aactttttat
     5401 gcgcaattgc gacctgtcaa tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca
     5461 tttgtacttc attatgacta atacgtggaa cagattggcg ctcttgattt tcgccgtttt
     5521 atcgctgctg gtggcgggtg aattgcgggc aggagtggtg gtcggcggaa cgcgatttat
     5581 ctttccggca gacagagaat cgatatctat tttactgact aataccagtc aggaatcctg
     5641 gcttattaac agtaaaatca accgcccaac gcgttgggcc gggggggaag cgtcgacagt
     5701 gccagcacca ttactggccg ctccgccact tattctcctg aagcccggta ctacaggcac
     5761 gttgcgcttg ctgagaacgg aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga
     5821 gttaagcatt gccagcgtgc catccggcaa agttgaaaat cagagcgtaa aagtggcgat
     5881 gcgctcggta tttaaactgt tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc
     5941 ttatcaacaa ttacgctgga cacggaattc cagggtgtac aactcactaa cccaacgcct
     6001 tactacatta acctgattca ggtgagcgta aatggtaaag cgttaagtaa tgcgggagtt
     6061 gtgccgccta aaagccagcg tcagacaagc tggtgtcagg caatcgcacc gtgtcatgtc
     6121 gcctggcggg cgataaatga ttacggcggg ttgagtgcaa agaaggagca aaatctgccc
     6181 tgaaacaggt tcggaaaacg tttgcgtttt ttttgccgca ggtcaattcc cttttggtcc
     6241 gaactcgcac ataatacgcc cccggtttgc acaccgggaa tccaggagag ttcatgtact
     6301 accccttcgt tcgtaaagcc cttttccagc tcgatccaga gcgcgctcat gagtttactt
     6361 ttcagcaatt acgccgtatt acaggaacgc cgtttgaagc actggtgcgg cagaaagtgc
     6421 ctgcgaaacc tgttaactgc atgggcctga cgtttaaaaa tccgcttggt ctggcagccg
     6481 gtcttgataa agacggggag tgcattgacg cgttaggcgc gatgggattt ggatcgatcg
     6541 agatcggtac cgtcacgcca cgtccacagc caggtaatga caagccgcgt ctctttcgtc
     6601 tggtagatgc cgaaggtttg atcaaccgta tgggctttaa taatcttggc gttgataacc
     6661 tcgtagagaa cgtaaaaaag gcccattatg acggcgtcct gggtattaac atcggcaaaa
     6721 ataaagatac gccagtggag cagggcaaag atgactatct gatttgtatg gaaaaaatct
     6781 atgcctatgc gggatatatc gccatcaata tttcatcgcc gaatacccca ggattacgca
     6841 cgctgcaata tggtgaagcg ctggatgatc tcttaaccgc gattaaaaat aagcaaaatg
     6901 atttgcaagc gatgcaccat aaatatgtgc cgatcgcagt gaagatcgcg ccggatcttt
     6961 ctgaagaaga attgatccag gttgccgata gtttagttcg ccataatatt gatggcgtta
     7021 ttgcaaccaa taccacactc gatcgttctc ttgttcaggg aatgaaaaat tgcgatcaaa
     7081 ccggtggctt aagtggtcgt ccgcttcagt taaaaagcac cgaaattatt cgccgcttgt
     7141 cactggaatt aaacggtcgc ttaccgatca tcggtgttgg cggcatcgac tcggttatcg
     7201 ctgcgcgtga aaagattgct gcgggtgcct cactggtgca aatttattct ggttttattt
     7261 ttaaaggtcc gccgctgatt aaagaaatcg ttacccatat ctaattattt cttcgaattc
     7321 ctctttataa gataatcagg gctttatttt cagccctggt tgttttatat tcatcgctgt
     7381 tgcttattta gacattttgt acttttattg ttgaggttat taagcgaagc gacaatggat
     7441 tgtgttgctg cggttttata gggtagggga gaggcagatg cgaattaaac cagacgataa
     7501 ctggcgttgg tattacgatg aagagcacga tcgaatgatg ctcgatttag ccaatggtat
     7561 gctatttcgc tcacgttttg cgcgcaagat gttgactcca gatgcctttt ctcccgcagg
     7621 cttttgcgtt gacgacgccg cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa
     7681 tttatctaaa gaacaaaaag ccgaactggt gctgaatgca ctggtagcaa ttcgctatct
     7741 caaaccgcaa atgccgaaaa gctggcattt tgtttcccat ggtgaaatgt gggtgcccat
     7801 gccgggcgat gcggcctgcg tttggttgag cgatactcac gagcaggtca atttgctggt
     7861 cgttgaatct ggcgaaaatg ccgcactatg cctgctggca caaccctgcg ttgttattgc
     7921 gggtcgcgcg atgcagttgg gcgacgccat taaaatcatg aacgacaggc tgaaaccgca
     7981 ggttaatgtt gacagcttca gcctcgaaca ggcagtctaa cgcgccaact taagtgcagt
     8041 cttcggtaca cagctacagc aaagaatggt gccatcatcg cccattgctg attttttcag
     8101 cggcatgact tcgccttcta aaagctgaac acggcaactt ccacaaatgc ccgcgcggca
     8161 agaataaggg atacgaattc cctgattttc taattgctcc agcaacacct gttggttatt
     8221 tccacgaaat gcctgtccct gccagtcaat atctacattt gcgtccggtt gttgcgtgat
     8281 gttggcggta tcatcagcgg cagctgcgcc gtaaattttt gccggagccg ttgccagaat
     8341 ttccacctca tcgccaacgc gaatcacgcc gctattacgg gcaattaaat tctgaccaaa
     8401 atcgacatcg ccgttatcct gggcagtgcg gaaagattgc aatgttttta atggttcgcc
     8461 tgccggatgt ttttgccctt tttctgggct gacggtggtg aaaatacagc ggctacaagg
     8521 tttaaccaca tcaaacacca catcaccaat gcgaatcact ttccagcgat cttcttccca
     8581 ggctgacgcg ccggaaacca ccagattggg gcggaattgc tccattttta cactggccgg
     8641 acaacgttgt tggagatcac gtaacgaggc ttcgttagca agaaggtaag gatagccatc
     8701 agcaaatgac agaggtacag tgttgtggcg tttcacgcgc cgggtcattt gtggccccac
     8761 ccagcgtaat tgcacttcgc gggagaaaaa tccacttagc catttgttga tcgcgtctgg
     8821 cgcaattcgc gcggtaaaat gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa
     8881 atcagcaaaa cgaacatatg cgctactgcc atctggtgcg gtgagatgca agccatcatg
     8941 cacaggtgaa ggagtaaacc gtaccatctg gggaaactgg cgagcggtaa taaacgtacc
     9001 gtcaggttcc gtgatcataa agatgcgatc gaaggccaga ccactgacat cagccagagc
     9061 atgtgtaaga ccaatgccgc gcatcgattt aacaggatga ataaaaagcc gggttaatgt
     9121 cgccacggtg cggtcctcaa atgaaaataa gccctcaact ttatgacatg acgcgcttat
     9181 tagctataat gcgcaacaat tttcttagcg cctgtcccat taggctattt tatttgtcag
     9241 tttggccctg ggttgtgctc gaaatcctca cgtacttgtg tacgctccgg tttctccgcg
     9301 ctgtccatgt ccagactgac tgcaacaatt acgcctactg cggtaggctc ttagagtaaa
     9361 agtgacgata tgaattctct gtttgccagt acggcccgtg ggctggaaga gctgttaaaa
     9421 actgaactgg aaaacctggg ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc
     9481 aagggcgaca cacggcttgt ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt
     9541 atgttgccgc tgggcgagtg taaggtttac agcgatttag acctctatct cggtgttcag
     9601 gcgatcaact ggacagagat gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt
     9661 ttgaatgaca ccatccgcaa cagtcagtac ggcgcgatga aagtgaaaga cgcgatcgtc
     9721 gatgctttca cgcggaaaaa tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc
     9781 cgcgttaacg tctggctgca taaagaaacc gccagtatcg ctcttgatct gagtggtgat
     9841 ggtttacatc tgcgtggcta tcgcgatcgt gctggtattg cgccgatcaa agaaaccctg
     9901 gcagccgcta ttgtgatgcg aaccggctgg cagccaggaa caccgctgct cgatccgatg
     9961 tgtggttccg gtacgttgct gattgaagcg gcgatgctag cgaccgatcg cgcaccaggc
    10021 ttgcaccgtg ggcgttgggg ctttagcggc tgggtgcagc atgatgaagc tatctggcag
    10081 gaagtgaaag cggaagcgca aactcgtgcc cgtaaaggcc tggctgagta tagctctcat
    10141 ttttacggtt cggacagcga cgcacgggtg attcaacgtg cgcgcactaa cgcccgtctt
    10201 gcggggattg gtgaactgat cacctttgag gtgaaagatg tcgcgcaact gaccaatccg
    10261 ctgccgaaag ggccgtacgg cacagtgttg agcaacccgc catacgggga acgtctggac
    10321 agcgaaccgg cgctgattgc gctgcatagc ctgctgggtc ggatcatgaa aaaccagttt
    10381 ggtggctgga atctctcttt gtttagtgcc tcgccggatc tgctaagctg cttgcagctg
    10441 cgtgcagaca aacagtacaa ggcgaaaaac ggcccgctgg actgcgtaca gaaaaattac
    10501 cacgttgccg aaagcactcc agacagcaaa ccggcgatgg tagcggaaga ctacgccaac
    10561 cgcctgcgta agaacctcaa aaaattcgag aagtgggctc gccaggaagg aattgaatgt
    10621 taccgcctgt atgacgccga tctaccggaa tataacgttg ccgttgaccg ttatgccgac
    10681 tgggtggtgg tgcaggagta tgcgccgcca aaaactattg atgctcacaa agcgcgtcag
    10741 cgtctgttcg atattatcgc tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg
    10801 gtgctgaaaa cccgtgaacg ccagaagggc aaaaaccagt accagaaact gggcgagaag
    10861 ggcgagtttc ttgaagttac cgaatataac gctcacttgt gggtgaacct gacggattat
    10921 ctcgatactg gtctgttcct cgatcaccgt atcgcccgcc gtatgctcgg tcagatgagc
    10981 aaaggcaaag atttcctcaa cctgttctct tataccggta gcgccaccgt gcatgcggga
    11041 ttaggcggcg cgcgcagcac caccaccgtg gatatgtcgc gtacttatct ggagtgggca
    11101 gaacgcaacc tgcgtctgaa tggcctgacc gggcgtgcgc atcgcctgat tcaggccgat
    11161 tgcctggcgt ggctgcgtga ggcaaatgaa cagtttgatc tgatctttat cgatccgcca
    11221 accttctcta actcaaaacg aatggaagat gcgtttgatg ttcagcgcga tcatatggcg
    11281 ctgatgaaag atttgaaacg tctgctgcgt gcaggtggga cgatcatgtt ctcgaacaac
    11341 aaacgtggct tccgtatgga tctcgacggc ctggcaaaac tgggactgaa agcacaagaa
    11401 attacgcaaa aaacgctctc ccaggatttc gcccgtaacc gccagatcca caactgctgg
    11461 ctgattaccg cagcctgaaa ggaatagtaa tgtcattaat cagtatgcat ggcgcatggc
    11521 tgtcgttcag cgacgcgccg cttctcgata acgcagaact gcatatcgaa gataacgaac
    11581 gtgtttgtct ggtgggtcgc aacggcgcgg gcaaatcgac gttaatgaaa atcctcaacc
    11641 gtgaacaagg gctggatgac ggtcgcatta tttacgagca agatttgatt gtagctcgtc
    11701 tgcaacagga gccgccgcgt aacgttgagg gtagcgttta tgatttcgtt gccgaaggca
    11761 ttgaagaaca agcggaatat ctgaaacgct atcacgatat ttcgcacctg gtgatgaacg
    11821 acccgagcga gaaaaatctc aacgaactgg cgaaggttca ggaacagttg gatcaccaca
    11881 acctgtggca gctggaaaac cgcatcaacg aagtgctggc gcaactgggg ttagatccta
    11941 atgttgcgct gtcgtcgctt tccggcggct ggttgcgtaa agcggcatta ggacgcgcgc
    12001 tggtgagtaa tccgcgcgtg ctgttgcttg atgaaccgac aaaccacctg gatattgaaa
    12061 ccatcgactg gctggaaggg ttccttaaaa ccttcaatgg gacgattatt ttcatctccc
    12121 acgaccgttc gtttatccgc aatatggcga cgcgcattgt tgatctcgat cgcggcaagc
    12181 tggtgaccta tccagggaat tacgaccagt acctgcttga aaaagaagaa gccctgcgcg
    12241 tggaagaact acaaaatgcc gagttcgatc gcaaactggc gcaggaagag gtgtggatcc
    12301 gccaggggat caaagcacgc cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcaa
    12361 tgcgtcgcga gcgtggtgaa cgtcgcgaag tgatgggtac cgcaaagatg caggtggaag
    12421 aggccagccg ctccggtaag atcgttttcg aaatggaaga cgtttgctac caggttgacg
    12481 gtaagcaact ggtgaaagat ttttcggccc aggttctacg tggcgacaaa attgccctga
    12541 ttggtccgaa tgggtgcggc aaaaccacgc tgctgaaact gatgctcgat cagcttcaag
    12601 cggacagcgg gcgtattcac gttggcacca aactggaagt ggcttatttc gatcagcacc
    12661 gcgcggaact ggatcccgat aaaacggtga tggataacct tgccgaaggt aagcaagagg
    12721 tgatggttaa cggcaagcca cgccacgtat tgggctattt gcaggacttt ctgttccatc
    12781 cgaaacgggc gatgacgccg gtacgtgcgc tttctggcgg tgagcggaac cgcttgctgc
    12841 tggcgcgttt gttcctcaaa ccaagcaact tattgattct tgacgaaccg accaacgatc
    12901 ttgatgtcga aacgctggaa ctgctggaag aactgatcga cagctatcag ggcacggtat
    12961 tgctggttag ccacgatcgt cagtttgtcg ataacaccgt tacagaatgt tggatcttcg
    13021 aaggcggcgg taaaattggt cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag
    13081 agcagtatgt ggcgctcaaa cagcctgcgg tgaaaaaaac cgaagaagcc gccgcggcaa
    13141 aagcagaaac tgtaaaacgc agcagtagca aactaagcta taaattgcag cgcgaactgg
    13201 agcagctacc gcaattgctc gaagatctgg aggcgaagct ggaagcccta cagacgcaga
    13261 tggcggatgc ttccttcttc agtcagccac atgagcagac acaaaaagtg ctcgccgata
    13321 tggctgctgc agagcaggag ctggagcaag cctttgaacg ctgggagtat cttgaagcgt
    13381 taaaaaatgg tggctgatcg caaaatagtc gatttaaaca gagacagctg acaacggtaa
    13441 aattgcctga tgaactgggt ttatcaggca tgaaaaccaa cactttgtta gccatctgta
    13501 aaatagcgca tcattaagga gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc
    13561 tgtgctcgca gtgtgacatg ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg
    13621 catgtccccg gtgtggcaca acgttaaccg tggcgtggga tgcccctcgg cagcgtccga
    13681 ccgcctatgc gttggctgca ctgttcatgc tgttgctgtc caacttgttt ccttttgtga
    13741 atatgaacgt tgcaggagtt accagtgaaa ttacattact ggaaattccc ggcgtgcttt
    13801 tttctgagga ctacgccagc ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg
    13861 cgttttgtct gataaccatt ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa
    13921 aagagcaact ggcacgggtg ctttttcaac tcaaaacctg gggaatggcg gagattttcc
    13981 tcgctggtgt gctggtcagt ttcgttaaac tgatggctta cggcagcatt ggggtaggca
    14041 gcagctttct cccctggtgt ttattttgtg tcctgcaact gcgcgctttt cagtgcgttg
    14101 atcgtcgctg gttatgggac gacatcgccc cgatgccaga actgcgccag ccgctaaaac
    14161 caggcgtcac ggggatacgt caggggctgc gttcgtgctc ctgttgtacg gcaatccttc
    14221 ctgctgatga acccgtgtgc ccgcgttgta gtaccaaagg gtacgttcgg cgtagaaaca
    14281 gccagcagtg gacactcgcg ctgcttgtaa cgtccatcat gctgtatctt ccggctaata
    14341 ttttgcccat catggtgacg gatttattag gctcgaagat gccgtcgacg attctcgctg
    14401 gggtcattct gttatggagc gaaggctctt atcccgtcgc tgcggtgatc tttctggcca
    14461 gtattatggt gccaacgtta aagatgatcg ccatcgcgtg gctgtgttgg gatgccaaag
    14521 ggcatggcaa gcgcgacagt gaaagaatgc atttgattta tgaagttgtt gagtttgtag
    14581 gccgctggtc gatgattgac gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg
    14641 gaggtttaat gagtatttat ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa
    14701 tgacaatgtt ttctgctatg acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat
    14761 cagagcatga ggagtcctga cagtatggaa tctaataatg gggaagccaa aatccagaaa
    14821 gtgaagaact ggtctcccgt gtggatattt cctatcgtca cggcgctcat tggggcctgg
    14881 gttctttttt atcattacag ccatcaggga ctggaagtga ccctgatcac cgcgaatgcg
    14941 gaaggaattg aaggtggcaa aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt
    15001 gaaagcgcca cactggctga tgatttgacg cacgttgaaa tcaaagtgcg gctgaattct
    15061 ggtatggaaa aattgctgca taaagacacc gtcttttggg tggtgaaacc gcagattggt
    15121 cgcgaaggga ttagcggcct gggaacgctg ctgtctggag tttatatcga actgcagcca
    15181 ggcgcgaaag gcagcaaaat ggataaatac gatttgctgg actcgccacc gttggccccg
    15241 cctgatgcga aaggtatccg tgtgattctc gatagcaaaa aagccgggca gctctcgcca
    15301 ggagatccgg tgctgttccg tggctatcgg gtaggttcgg ttgaaaccag caccttcgat
    15361 acgcaaaaac gcaatatcag ttatcaactg ttcatcaatg caccttatga ccgactggtg
    15421 accagcaatg ttcgcttctg gaaagatagt ggcattgcgg ttgatctgac gtcagcgggg
    15481 atgcgtgtgg agatgggctc attgacaacg ctgcttagtg gcggtgttag ctttgatgtg
    15541 ccggaaggtc tggatttagg gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat
    15601 gatcagaaga gcattcagga ttcgttgtac accgatcaca ttgattatct gatgttcttt
    15661 aaagattcgg tacgcggtct gcaaccggga gctccggtag aattccgggg tattcgcctg
    15721 ggtaccgtaa gcaaagtgcc attctttgcg ccgaatatgc gtcagacatt taacgatgat
    15781 taccgtattc cggtactgat tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa
    15841 aatgcggatg ttgttgagca ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg
    15901 aaaaccggaa acctggtcac tggtgcactg tatgttgatc ttgatttcta tccaaatacg
    15961 cctgcaataa ccggtattcg tgaatttaat ggttatcaga ttatcccgac tgttagcggc
    16021 ggcctggcgc aaatccagca acgactgatg gaagcgttgg ataagatcaa caaactgcca
    16081 ttgaatccga tgattgaaca ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa
    16141 aacctgcaaa cgacgctgga tagcatgaac aagatcctcg ccagccagtc gatgcagcag
    16201 ttgccgacgg atatgcagtc aacgttgcgt gaattgaatc gcagcatgca gggcttccag
    16261 cctggctccg cagcctacaa caagatggtg gcggatatgc agcgccttga tcaggtgttg
    16321 cgagaactgc aaccggtgct gaaaacgctc aatgagaaga gtaacgcgct ggtatttgaa
    16381 gcgaaggaca aaaaagatcc agagccgaag agggcgaaac aatgaaaaag tggctagtga
    16441 cgattgcagc actgtggctg gccggatgca gctccggcga aattaataaa aactattacc
    16501 agttacctgt ggtgcagagc ggtacacaaa gtaccgccag ccagggcaat cgtctgttat
    16561 gggtagagca ggtcactgtt cctgactatc tggcggggaa tggtgtggtt tatcaaacca
    16621 gtgatgtgaa gtatgtgatt gccaacaaca acttgtgggc cagcccgttg gatcaacaat
    16681 tgcgcaacac cctggttgcc aacctgagta cgcaactgcc cggctgggtg gttgcctccc
    16741 agcctctggg aagcgcccag gacacgctca atgttaccgt aacggagttt aacggtcgct
    16801 atgatggcaa ggtcattgtc agtggtgagt ggctgttgaa ccaccaggga caactgatca
    16861 aacgtccgtt ccgtctggaa ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag
    16921 tactggccag tgtctggagt caggaagccg cttctattgc gcaagagata aagcgtctac
    16981 cttaattata aagatttgta aatataaccg tctccggtat gttgcctgag gcggtttttt
    17041 tgtttctaac gtgcggaaaa agttgttcct ctttacattt tttgtacaac cgacataccc
    17101 gtgtagctca caaatatgac agtggcgtga attttgcgca ttgacggcag ttatgattcg
    17161 cggtattgct taactgtgat tgcacattta gtaatcactg ttttcttttc caccagaaac
    17221 cagtatgagg gaaacgaggc atgaagagac aaaaacgaga tcgcctggaa cgggcacatc
    17281 aacgtggtta tcaggccggc atcgccggac gctcaaaaga aatgtgtccc tatcagacgc
    17341 tgaatcaaag gtcacaatgg ctgggaggct ggcgagaagc catggcggac agggtagtaa
    17401 tggcctgatt ctgtctcttt aaaaagaaac ctccgcattg cggaggtttc gccttttgat
    17461 actctgtctg attataatca gaaggcagaa gtatcctgga acagaccgac tttcaggtcg
    17521 ctggcggtat agatcaaacg accatcaacc agcacttctc catccgccag gcccataatc
    17581 agacgacggt taacaatgcg tttaaagtga atacggtagg tcactttttt cgctgtcggc
    17641 agtacctgac cagtgaattt cacttcgcca acgcccagtg cgcggccttt accttcgccg
    17701 cccagccagc cgaggtagaa ccctaccagc tgccacattg cgtccaggcc caggcatccc
    17761 ggcataaccg gatcgccaat aaagtggcat ccgaagaacc acagatccgg attgatatcc
    17821 agttctgctt caacataccc tttgtcgaag ttaccacccg tttcggtcat tttgaccaca
    17881 cggtccatca tcagcatatt cggtgctggc aattgcgggc ctttagcgcc aaacagttca
    17941 ccgcgaccag aggcaagaag gtcttctttt gtataggatt cgcgtttatc taccatgttc
    18001 tctgtaagcc ttattttatt gaagcacgca ggatagctaa cacgtgtacg ctgaacaagt
    18061 ccgatcagtt cggaataaac cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc
    18121 cttcctgttg cgatgcttgc gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc
    18181 cttcgccatc ccacaccaga tttaataata acggcagtgc gtcagtcaca tcgtctactg
    18241 cccagatggt gaatttgcct tcttctaccg ctttcactag ttcactgtga agacttaaat
    18301 ggcgaacgtt agctgtgggg ataatgacac cttgtttccc ggttaactca cgttgctggc
    18361 aaatagcaaa gaagccttcg attttctcat ttaaaccacc gaccggctgg gcgcgaccga
    18421 actgatcgac tgaacctgtg atagcgatac tctgattcac cggcacatcg gcgagggcgc
    18481 ttatcagggc gcagagttca gccatcgagg cactatctcc atcaacttca ctgtatgact
    18541 gctcaaatgt cagcgatgct gagaagggga tctgttgctc aagctgtagt tccgacatca
    18601 ggaacgcttg catgatcatc atccctttcg catggatatt gccgccaagc tccgctttgc
    18661 gttcgatgtc ggtgaattca ccatcgccaa tatgcacaac gcagctaatg cgagaaggtt
    18721 cgccaaaagc gcgtggatga cccggaaatt caatgaccga aagggcgtta atttgcccga
    18781 tgcgttcgcc ttcggtttca atcaggattt gctcctgaag gatctcatcc tgcatacgtt
    18841 cagcgaggaa accttcgcgc cattcacgct gctgcagcat taagtttagc tgctcgccgg
    18901 agaaggtgtc gccatcacac agggaggcga cctctttaca ctggcggagg atccactgcg
    18961 ggctaagcgg aagtgtttct tgttcaccgg tgtagcgtgc tgcttcgcgg ataagtaccg
    19021 gccaggcatc cgctcccggt gcaggtaagt gattatgtct ggcggtaaat gtcacccagc
    19081 gacaccactg ggttactgac tccgcatcga caatctgcag agtatcttca aattcgctat
    19141 aaatagcctg ctctgaaagc tctggctcca tctcctggaa atcagccaat gattcgcgtt
    19201 cgcctaccag aatgactttc agcttcaatg gcatcgaagg cacagagacg gggagagggc
    19261 gcgactcatc aaacgcaacc cagtcaaaac gctcgcggtt aacgatattt ttcagccgca
    19321 tccacagcag aggttgcgcc agcagtgtac gcaaagagat gatgagaata ccgccatttg
    19381 cctgatgcac cagaccaggc tgcagggtaa tgtcgccatt aaactggcgc aggcagccaa
    19441 agagttgctc cgcttctacc cagtcggcag cgacaacttg cgttaaagtc gcaaaattat
    19501 catctgcact cactgcgtga cgtaagcgga tggagtggcc ggaaacctca tagtgaccgc
    19561 ccaccagttg gcctgcatcg ctttgtagcg tacgcgcggc attggcaata agattcagat
    19621 actcagactc ttccggggcc ttcgccagca taaaagagga ggatgctcgc gtatgcagca
    19681 actgctccag cgcaaattgc agccgcggtt gagtatcact gaataaagga tcgttttcgt
    19741 caatcaaatg tggctgagca aatatttcct gatagctatc ggtatcagga accaggtcac
    19801 gccatgcaag tttcgtaatg gtcaaagttg atgtttttta gtctgttgtc aaagccgcga
    19861 ttataccgta accggcacta cagcacacgt agaaagcacc gacaatactc caggcatggg
    19921 cgttaaagct cacaggatgg agattctttt cttcactggc ctaaaaagct gatattctgt
    19981 aaagagttac acggtaacat tgagatcgct atgaaatatc aacaacttga aaatcttgaa
    20041 agcggttgga aatggaagta tctggtgaag aagcatcgcg aaggggagtt aatcacccgt
    20101 tacatagaag ccagtgccgc ccaggaagcc gtcgatgtgt tgctctcgct ggaaaatgaa
    20161 cccgtgctgg taaatggctg gattgacaag catatgaatc cggaactggt caatcgcatg
    20221 aagcaaacca ttcgggcaag acgaaagcgc cattttaatg cggaacacca gcatacgcgc
    20281 aaaaaatcga tcgatctgga atttattgtc tggcaacgtc tggctggtct tgcgcagcgt
    20341 cgcggtaaaa cgctttctga aacgattgtt cagctgattg aagatgcgga aaacaaagag
    20401 aaatacgcga ataaaatgtc ttctctgaag caggatctgc aggcattgct gggtaaggaa
    20461 taactgacga aagtcagttc aatttactaa aggcaaaaaa aaccccgcag cagcggggtt
    20521 tttctaccag acgagaactt aagcctgcgg ctgagttaca acgtctttga tacctttaac
    20581 ttcgatctct acgcgacgat ccggagccag gcagtcgatc agtgcagcac gctgtttcac
    20641 gttgtcacag gtgttgccag taaccgggtt ggattcgccc ataccacgtg cggagatctt
    20701 gtctgccggg atacctttgg agatcaggta atcaacaaca gactgagcac ggcgctcgga
    20761 cagaccctgg ttgtaagcgt cagaaccgat gcggtcggtg taacccagaa caactacgga
    20821 accgtctttc ggatccaggt tgctcagctg gctgtacagc tgatccagag cagcctgacc
    20881 ttccggtttc agggttgctt tgttgaagtt gaacagaacg tcagacttca gagtgaagtg
    20941 cttggtctgt acttccggtg ccggagctgg agccggagca actactggag ctgcttcgcc
    21001 ctgaccgaaa cggtaggaaa cccccaggct cagcatgccg ttgtccggac gagtgccgat
    21061 ggtgtgtgcg tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg
    21121 agtgatcgcg tactcaacac cgccagcgaa gaccggagaa acgccggtgt cgtggttttt
    21181 accataaacg ttggatttag tgtctgcacg ccataccata ccacccagac gagtgtagat
    21241 gtccaggtcg tcagtgattg ggtaacccag tttagcggtc agttgaacgc cctgagcttt
    21301 gtatgcaccg ttttcaacgc tgcctttgta cggcatacga cctaaccagt cgtaacccat
    21361 ttcaaagcca acatacgggt taacctggta accaccaaaa gcaccagcgc ccagttggtt
    21421 ttcatgggtc gggccattgt tgttgatgaa accagtgtca tggtactggg accagcccag
    21481 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21541 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21601 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21661 tgctcaatta actctaccga tatcttcggc ttatgccgag cacccctggc gatgtaaagt
    21721 ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa aaaaaagtct
    21781 tgtataaggt atgtttaatc ttttttgtca gcgacaattt acagaagaaa atcgcggaaa
    21841 ccgcttcaga caagcctccg caaggaaaat tagtcacgac tgaaagcatt ggctgggcga
    21901 caaaaaaagt tccaggatta atcctaaatt tacttaatga tacaaattag agtgaatttt
    21961 tagcccggaa agttgtctcg tggcgtgaga ggatgcgctt accggacgca taataaaccc
    22021 catagcgtta ccttcatttg ccgcatcaac aagttcagca tgctcttctt cagtcaaatc
    22081 atctgccaac caaccgatca ccacactgta attgcccgtg cgtaaagcgc gaaccattga
    22141 ctccacagtg tggcaagggg agagctggct aatctgcatt actttcgtta agggtagccc
    22201 agatgcctga acccattccc gactcagttt ttgttgcggt gttaaccaga gttgccagcg
    22261 cgattgctga ccgagttgct gtaacaatgg caacagtaga agttgcgtca tcatgggctg
    22321 atcttcgcga tagacaactt cactgataag cccggctgta gtgttttccg tagagacacg
    22381 cgcaatttta cttgctgcgg atgagaacga cgaagaacga tgtgcatagc ctgaagtgta
    22441 cataatcaat ccagccccta tgagttactg tatggatgta cagtacatcc agtgacaaca
    22501 aagatcaacc ctattttcgg aaagagcctc gcaaattttg tcgttggtga cgggaaaaca
    22561 taaattaatc ttgcccctta agaataagtt gcctattttc gtag
//
LOCUS       DAZOZD010000047        22578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOZD010000047 DAZOZD010000000
VERSION     DAZOZD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.047"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHUPB_001461"
     CDS             complement(<1..130)
                     /locus_tag="ACHUPB_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0758316.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVIN"
     gene            complement(140..691)
                     /gene="ybcL"
                     /locus_tag="ACHUPB_001462"
     CDS             complement(140..691)
                     /gene="ybcL"
                     /locus_tag="ACHUPB_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0758317.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(860..1192)
                     /gene="emrE"
                     /locus_tag="ACHUPB_001463"
     CDS             complement(860..1192)
                     /gene="emrE"
                     /locus_tag="ACHUPB_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0758318.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1373..1501
                     /locus_tag="ACHUPB_001464"
                     /pseudo
     CDS             1373..1501
                     /locus_tag="ACHUPB_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1526..1840)
                     /gene="fliE"
                     /locus_tag="ACHUPB_001465"
     CDS             complement(1526..1840)
                     /gene="fliE"
                     /locus_tag="ACHUPB_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0758319.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2056..3714
                     /gene="fliF"
                     /locus_tag="ACHUPB_001466"
     CDS             2056..3714
                     /gene="fliF"
                     /locus_tag="ACHUPB_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR0758320.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3707..4702
                     /gene="fliG"
                     /locus_tag="ACHUPB_001467"
     CDS             3707..4702
                     /gene="fliG"
                     /locus_tag="ACHUPB_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0758321.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4695..5381
                     /gene="fliH"
                     /locus_tag="ACHUPB_001468"
     CDS             4695..5381
                     /gene="fliH"
                     /locus_tag="ACHUPB_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0758322.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5381..6754
                     /gene="fliI"
                     /locus_tag="ACHUPB_001469"
     CDS             5381..6754
                     /gene="fliI"
                     /locus_tag="ACHUPB_001469"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0758323.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6773..7216
                     /gene="fliJ"
                     /locus_tag="ACHUPB_001470"
     CDS             6773..7216
                     /gene="fliJ"
                     /locus_tag="ACHUPB_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR0758324.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7213..8340
                     /locus_tag="ACHUPB_001471"
     CDS             7213..8340
                     /locus_tag="ACHUPB_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0758325.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8445..8909
                     /gene="fliL"
                     /locus_tag="ACHUPB_001472"
     CDS             8445..8909
                     /gene="fliL"
                     /locus_tag="ACHUPB_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0758326.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            8914..9918
                     /gene="fliM"
                     /locus_tag="ACHUPB_001473"
     CDS             8914..9918
                     /gene="fliM"
                     /locus_tag="ACHUPB_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0758327.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9915..10328
                     /gene="fliN"
                     /locus_tag="ACHUPB_001474"
     CDS             9915..10328
                     /gene="fliN"
                     /locus_tag="ACHUPB_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0758328.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10331..10696
                     /gene="fliO"
                     /locus_tag="ACHUPB_001475"
     CDS             10331..10696
                     /gene="fliO"
                     /locus_tag="ACHUPB_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0758329.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10696..11433
                     /gene="fliP"
                     /locus_tag="ACHUPB_001476"
     CDS             10696..11433
                     /gene="fliP"
                     /locus_tag="ACHUPB_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR0758330.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11443..11712
                     /gene="fliQ"
                     /locus_tag="ACHUPB_001477"
     CDS             11443..11712
                     /gene="fliQ"
                     /locus_tag="ACHUPB_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0758331.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11721..12506
                     /gene="fliR"
                     /locus_tag="ACHUPB_001478"
     CDS             11721..12506
                     /gene="fliR"
                     /locus_tag="ACHUPB_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0758332.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12791..13414
                     /gene="rcsA"
                     /locus_tag="ACHUPB_001479"
     CDS             12791..13414
                     /gene="rcsA"
                     /locus_tag="ACHUPB_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0758333.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13458..13646)
                     /gene="dsrB"
                     /locus_tag="ACHUPB_001480"
     CDS             complement(13458..13646)
                     /gene="dsrB"
                     /locus_tag="ACHUPB_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0758334.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13809..14036
                     /gene="yodD"
                     /locus_tag="ACHUPB_001481"
     CDS             13809..14036
                     /gene="yodD"
                     /locus_tag="ACHUPB_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0758335.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14334..15149
                     /locus_tag="ACHUPB_001482"
     CDS             14334..15149
                     /locus_tag="ACHUPB_001482"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR0758336.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15146..16840)
                     /gene="dgcQ"
                     /locus_tag="ACHUPB_001483"
     CDS             complement(15146..16840)
                     /gene="dgcQ"
                     /locus_tag="ACHUPB_001483"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR0758337.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17011..17193)
                     /locus_tag="ACHUPB_001484"
     CDS             complement(17011..17193)
                     /locus_tag="ACHUPB_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0758338.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17272..18189)
                     /locus_tag="ACHUPB_001485"
     CDS             complement(17272..18189)
                     /locus_tag="ACHUPB_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0758339.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18362..19283
                     /gene="yedA"
                     /locus_tag="ACHUPB_001486"
                     /pseudo
     CDS             18362..19283
                     /gene="yedA"
                     /locus_tag="ACHUPB_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19272..19742)
                     /gene="vsr"
                     /locus_tag="ACHUPB_001487"
     CDS             complement(19272..19742)
                     /gene="vsr"
                     /locus_tag="ACHUPB_001487"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0758340.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19723..21141)
                     /gene="dcm"
                     /locus_tag="ACHUPB_001488"
     CDS             complement(19723..21141)
                     /gene="dcm"
                     /locus_tag="ACHUPB_001488"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0758341.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21208..21864)
                     /locus_tag="ACHUPB_001489"
     CDS             complement(21208..21864)
                     /locus_tag="ACHUPB_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR0758342.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21904..22185)
                     /gene="drpB"
                     /locus_tag="ACHUPB_001490"
     CDS             complement(21904..22185)
                     /gene="drpB"
                     /locus_tag="ACHUPB_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0758343.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5537 a         5927 c         5705 g         5409 t
ORIGIN      
        1 aattgataac tttttttccc tgacggatac gacatgaagc tggcataaga ggcttaaccc
       61 tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta gtcctatctt
      121 ttgccagcat actcttatcc tactttacct cataaacggg tattatctca gcggttgcaa
      181 ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa
      241 tcttatcagt ttttagagcc catattttaa actggtaatg atggggttta tctcctttgg
      301 gaggacatgc tccaccaaat cccgcatagc caaaatcatt tcggccttgg acagcaccag
      361 tcggaagttt tgtaccatca cgtcttcctg catcagcggg caaatatgtt accgttgctg
      421 gaatattagc aacagtccag tgccaccatc cgctgcctgt aggcgcatct ggatcataca
      481 cagttacggc aaagcttttg gtgccttctg gagcaccaga ccaggttaat gagggcgatg
      541 tattaccccc ctcacaccca actccagaaa atacatgaga cgttgtaagt tgctctcctg
      601 tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg
      661 ccaatacact tgaaacgata agttttttca taaaaacctc tttgttgtga cctatcgtta
      721 ttttatttga tgtgcattga tctcattatg caaaaaggcg caatgatcgc gcaaatgcaa
      781 tcataattat aaagttgccc cgaacattag ccataatatg cacaaatcag attcctgtta
      841 tctcaaggat gattattttt taatgcggtg cgcttcgtga caacaagtta atgactaaca
      901 caccggcaca aatcaacatc atgcctatga tggctggcag gtccagtcgt tggccaaaaa
      961 atccccagga aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa
     1021 tccctgtagg tatataagcc agtgtctgag ctaataacca aaatgatgca caatagcaaa
     1081 taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg
     1141 taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt
     1201 ctttcctgtt catactggag agaattgtac tacagtttga acacaactca ccgttttcat
     1261 cctggtgtat gtgtatccag ggggctctgg cgtggcaaga gataaaggca ggaacaataa
     1321 tgatcagaag gaaccccagg ctaacaaaag agttcactct tcctatgagc aaagcgaaga
     1381 caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aactttcagg atcaggtatc
     1441 cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgcgg tgagatcgga
     1501 tgtaattaac aacaggttac aaaacctaca cctgcatgga catcacttcc tgataagccg
     1561 ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat
     1621 cggtcatcac atcgtttaac gccacgccgg gttcaccgag agtgaatttt tctgcctgcg
     1681 tgcgggcggc tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc tgcccggcaa
     1741 aactaatggt cggttgtggc agtgattcct gcgcacgcgc actcatcgcc gtagcctgta
     1801 actggctgat aaccccttca atcccctgta tcgctgacat tttcatctcc cggataattt
     1861 ctggtagcaa agcttaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa
     1921 acgggtttat tggcggatag aaaaaaacga aagcacaaat aatggcagcg tcaatttttc
     1981 gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca
     2041 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
     2101 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc agtggcggtc
     2161 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
     2221 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttactgc
     2281 ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga actgcgtctg
     2341 cgcctggcac aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact gcttgatcag
     2401 gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc
     2461 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
     2521 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
     2581 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcacctggtt
     2641 tccagcgccg ttgctggtct gccgccggga aacgtcacgc tggtggatca gggcggacat
     2701 ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc
     2761 agcgatgtcg aaggccgtat tcagcggcgt attgaagcaa tcctgtcgcc tattgttggt
     2821 aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg
     2881 gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt
     2941 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
     3001 caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc
     3061 cagcagcagg cgagcaccac cagcaatagc gggccgcgta gcacacagcg gaatgaaacc
     3121 agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa
     3181 cgtctgtcag tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct
     3241 ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaagcgat gggcttttct
     3301 gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt tcaatagcag tgacgaaagc
     3361 ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagttgct ggctgccggt
     3421 cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc ggaaagcggt acgcccgcag
     3481 ttaacacgtc gcgccgaggc gatgaaagct gtacagcaac aggcgcaggc ccgtgaggaa
     3541 gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactccaaca acgacgcgct
     3601 aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     3661 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     3721 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
     3781 gttcaagcac ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac
     3841 gcagatctcc aacaagcagc taacctatgt gctggcggag tttgagcaag aagctgaaca
     3901 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     3961 gggtgaggaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg ataccgccag
     4021 cggtattgaa acgctcaact ttatggagcc gcagagtgcc gccgatctga ttcgcgatga
     4081 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcgcagg ccgccgatat
     4141 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     4201 cggcgtgcag ccagccgcgc tggcggaact gaccgaagta ctgaatggct tgctcgacgg
     4261 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     4321 gatgaaaact cagcaggaag aagccgttat taccgccgtg cgcgaattcg acggcgagct
     4381 ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat ctggtggatg tcgacgatcg
     4441 cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg
     4501 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     4561 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
     4621 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag
     4681 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg
     4741 cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc attgaagagg
     4801 ccgaacctag ccttgagcag caactggcgc aactgcaaat gcaggcccat gagcaaggtt
     4861 atcaggcggg gattgccgaa ggtcgccagc aaggtcatga gcagggctat caggaaggac
     4921 tggcccaggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg
     4981 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga
     5041 ttgcttcgcg cctgatgcag atggcgctgg aggcggcacg tcaggtgatc ggtcagacgc
     5101 caacagtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     5161 tattcagcgg taaaccacag ctgcgcgtgc acccggatga tctgcaacgt gtggatgata
     5221 tgctcggcgc aaccttaagt ttgcatggct ggcgcttgcg gggcgatccc acactccatc
     5281 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct
     5341 ggcaagaact ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg
     5401 ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg
     5461 ctatgggcga ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
     5521 gctcggcgca acctgtgtca ttgaacgcca gaacggcagc gaaacgcacg aagtagaaag
     5581 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg
     5641 tgtcctgccc ggcgcgcggg tttatgccaa aaacatttcg gctgaaggac tgcaaagcgg
     5701 caagcagttg ccgctcggtc cggcgttatt aggtcgcgtt ctggacggca gcggtaagcc
     5761 gctcgatggc ctgccctccc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt
     5821 taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
     5881 tgccctgctt accgttgggc gtgggcagcg tatggggctg tttgccgggt ccggcgttgg
     5941 taaaagtgtg ctgctgggga tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg
     6001 tttgattggt gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgctga
     6061 agggcgtgca cgctccgtgg tgattgccgc tccggcggat gtctctccgc ttctgcgaat
     6121 gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt
     6181 gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc
     6241 gattggcgaa ccacccgcca ccaaaggtta tccaccgtcg gtgtttgcca aattaccggc
     6301 actggtcgag cgtgccggaa atggcattag cggcggcggc tcgattaccg cgttttatac
     6361 cgtgcttact gaaggcgatg accagcagga ccccattgca gactccgcac gggcgatcct
     6421 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
     6481 tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg
     6541 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
     6601 cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca
     6661 gctggagggc tatttacaac aaggcatttt tgaacgtgcg gactgggaag cgtctctcca
     6721 ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggt agatggcaga
     6781 acatggtgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg
     6841 cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa gaacagctca aaatgctgat
     6901 cgattatcag aatgaatatc gcaataacct caacagcgat atgagtgccg ggatgaccag
     6961 caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca
     7021 tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga
     7081 aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact
     7141 gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat
     7201 gaggaaacct gaatgattcg cttagcgccc ttgattaccg ccgacgttga caccaccaca
     7261 ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca
     7321 ttagcaagcg agacaactac cgacaaagcg gccccccagg tgctggtggc aacagataag
     7381 cccacgacaa aaggcgagct gctgatcagc gatattgttt ccgacgcgca acaagcggat
     7441 ttactgatcc ctgtggatga aacactgcct gtcatcaacg tcgaacaatc cacatcgaca
     7501 ccgttaacca ccgctcatac gatgacgttg gctgcggtgg ctgacaaaaa tacgacaaaa
     7561 gacgaaaaag cggatgatct gaatgaagac ctcaccgcaa gcctgagcgc cctttttgcg
     7621 atgttgccgg gttttgacaa tacgcccaaa gtgactgatg cgccgtcaac cgtgttaccg
     7681 gcagagaaac caacgctctt cacaaaactg acttctgcgc aactcacaac agcacagcct
     7741 gatgatgccc ccggcacgcc agctcagcca ttaacaccgc tggtagcaga agcccagagt
     7801 aaagcggaag tcatcagcac accttcaccg gtgaccgctg ccgccagccc gctaatcact
     7861 ccacaccaga cacagccact gctcaccgtc gccgcgcctg tcttgagtgc accgctgggt
     7921 tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg ccagggacaa
     7981 caaagtgcag agttgcgtct gcacccgcag gatttaggtg aagtgcaaat ctccctcaaa
     8041 gtggatgata accaggcgca aatccagatg gtttcgccgc atcagcacgt acgcgccgcc
     8101 ctggaagcag cgctgccggt actgcgtacg cagctggccg agagtggcat tcagttaggg
     8161 caaagcaaca tcagtggcga aagctttagt ggtcagcagc aggccgcttc ccaacaacag
     8221 caaagccaac gcacagtaaa ccatgaacct ctggcggggg aagaagacga tacgcttccg
     8281 gttcccgtct ctttacaagg gcgcgtaaca ggcaacagcg gcgttgatat tttcgcctaa
     8341 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
     8401 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
     8461 taagcaagaa aagcaagcga tcgctttggc tcccgattct ggtattcatt accctcgcgg
     8521 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gaagacaaag
     8581 cgcagcatcg cgtcgtgccc tcaccggtct tctatgcgct ggataccttt acggtcaatt
     8641 tgggcgatgc ggatcgcgtg ctttatatcg gtataaccct gcgactcaaa gatgaagcta
     8701 cccgctcgcg gctgagtgag tatttgccgg aagtccgtag ccgtttgctg ttactgtttt
     8761 cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
     8821 ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
     8881 tgatgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
     8941 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agccagtgtt
     9001 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
     9061 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
     9121 ttcaacctgc tgcgtcgtag cccggatata accgtcgggg ccatccgcat tcagccgtac
     9181 catgaatttg cccgcaacct gccggtgccg accaacctga accttatcca tctgaaaccg
     9241 ctgcgcggca cagggctggt ggtgttttca ccgagtctgg tgtttatcgc cgtggataac
     9301 ctgtttggcg gcaatggacg cttcccgacc aaagtggaag gtcgcgagtt tacccatacc
     9361 gaacagcgcg tcatcaaccg catgttaaag ctggcgcttg aaggctatag cgacgcctgg
     9421 aaggcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc
     9481 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
     9541 aacctgaccg gcgaatttaa tatctgtctg ccattcagca tgatcgagcc gctacgggaa
     9601 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
     9661 ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg ccaactttgc cgatatctcg
     9721 ctacgcctgt cgcagatttt aaaactgaaa cccggcgacg tcctgccgat agaaaaaccc
     9781 gatggcatca tcgcccatgt tgacggcgtc ccggtgctga ccagtcagta cggcactctc
     9841 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
     9901 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
     9961 acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac cagcagcaaa agcgctgccg
    10021 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
    10081 tgataatgga tattccggtc aagctgaccg tcgagctggg ccgtacgcgg atgaccatca
    10141 aagagctgtt gcgtctgacg caagggtccg tcgtggcgct ggacggtctg gcgggcgaac
    10201 cactggatat tctgatcaac ggttatttaa tctcccaggg cgaagtggtg gtcgttgccg
    10261 ataaatatgg cgtgcggatc accgatatca ttactccgtc tgagcgaatg cgtcgcctga
    10321 gccgttagtg atgaataacc acgctactgt gcaatcttcc acgccggttt ctgctgcgcc
    10381 actgctgcag gtcagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
    10441 gctggtaaaa cggttgggat ttgccccgaa acgcactggc gttaacggtc tgaaaattag
    10501 cgccagtgct tcgctgggcg cgcgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg
    10561 gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc ccccttcagc
    10621 accaacagaa gagataccgc agaccgattt tcagtcggtc atgaaaaatt tgcttaagcg
    10681 tagtgggaga tcctgatgcg tcgtttgttg tctgtcgcac ctgtccttct ctggctggtt
    10741 acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctgcc tggcggtgga
    10801 caaagctggt cgctcccggt gcagacgctg gtgttcatca cctcgttgac gtttattccg
    10861 gcaattttac tgatgatgac cagtttcacc cgcatcatca ttgtttttgg tttattgcgt
    10921 aacgcgctgg gaacgccctc cgcgccacct aaccaggtat tgctggggct ggcactattt
    10981 ttgacctttt ttattatgtc atcggtgatc gacaaaattt atgtcgatgc gtaccagcca
    11041 ttcagcgaag agaaaatatc aatgcaggag gcgctggaaa aaggggcgca gccgctacgt
    11101 gagtttatgc tgcgtcagac ccgtgaggca gatttagggt tgtttgccag actagcgaat
    11161 accggcccgt tgcagggacc tgaagccgtg ccgatgcgca ttttgctccc ggcctacgtg
    11221 accagcgaat tgaaaaccgc atttcagata ggcttcacga ttttcattcc ttttttgatt
    11281 atcgacctgg tgattgccag cgtgttgatg gcattgggga tgatgatggt cccccccgcc
    11341 accattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg
    11401 gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc
    11461 atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc gctgttgctg
    11521 gtggcgctgg tcacgggcct tatcatcagt attttgcagg ccgccacgca gatcaacgaa
    11581 atgacgctgt cgtttattcc aaaaatcatc gccgtattta ttgccattat tattgccgga
    11641 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
    11701 atcatcgggt aggccgtaca atgatgcagg tgacaagcga tcaatggctt tcctggttaa
    11761 gcctgtactt ctggccgtta ctgcgcgtgc tggcgctcat ttctaccgca ccaattctga
    11821 gcgaacgcag cgtgccgaaa cgggtaaaac tggggctggc aatgatgatc acgttcgtca
    11881 ttgccccatc attacctgcc aacgatgttc ctgttttttc gttctttgct ctgtggctgg
    11941 ccgtgcagca gatcctgatc ggcattgcgc ttggttttac catgcaattt gcctttgccg
    12001 ctgtgcgaac cgctggcgaa attatcggtc tgcaaatggg gctgtcattt gcgacgtttg
    12061 tcgatccggc cagccatctc aatatgcccg ttttagcgcg tatcatggat atgctggcgt
    12121 tactgctgtt cctgacattt aacggtcatt tatggttgat ttcactgctg gtcgatacct
    12181 ttcacaccct gccaattggt ggcgaaccgt tgaacagcaa tgcgtttctg gcactcacca
    12241 aagcggggag tttgattttc cttaacgggc tgatgctggc gttaccgctc attactctgc
    12301 tgctgaccct gaatctggca ttaggtttac tgaatcgtat ggccccgcaa ttatccattt
    12361 ttgttattgg atttccatta actctgactg tcggtatctc tttaatggcg gcattaatgc
    12421 cgttaattgc acctttttgc gaacatttat tcagtgagat gtttaatttg ctggctgata
    12481 ttattagtga attgccatta atataattcc gtaacgttta tcatgttatc ctaaggatta
    12541 tccgaaaaat aatacctacg aacatcttcc aggatactcc tgcggcgaaa tatttgtttt
    12601 aagctcactc acatatcgca acatttactt taagacaatt ccaggcaaat tatacaacac
    12661 tttacgggat agtaagtccg cctgaaaaat cgcgagagtg gcgcattagg tgacccatgt
    12721 tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac gcattgagtg
    12781 agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac taggtttaac
    12841 cgggtatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg aaaccgttga
    12901 tgaccttgcc atagcttgtg attcacagcg cccttcagtg gtgtttatta atgaggactg
    12961 tttcatccat gatgcttcta acagtcagca catcaagcac atcattaatc aacatcccaa
    13021 tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat atctattggt
    13081 cagaaaaaaa ttattgataa gttctaaatc gattaaaccg gaatctctcg acgatatcct
    13141 tggcgatatt ctgaaaaaag agacaacgat aacctctttt ttaaatatgc cgacgttatc
    13201 attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg gaaccattca
    13261 aatctctgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag gtaatattaa
    13321 acgtaagatc aaaacacata ataaacaggt tatctaccat gtcgtccgac tgacggataa
    13381 tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg gtttcccacc
    13441 agtcaggctg aataagatta ctctgctttc tccacaaaga taccgtcctg atgccctgct
    13501 tcattaaaga accagatgcc gagcgggtag tcttccagcg aaaccaggta cattgtgcct
    13561 tcactaaact cctcaactgc cagtaccacg ccaggacgac gcggaccgcc atccgttttg
    13621 actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa
    13681 caatttcgtt tttctggcag ctccaggcgc gcgcgagtgc tgattttctc gacggtctat
    13741 acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa cggttgcgag
    13801 gaaagatgat gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag gtcagcgtcg
    13861 atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt catcgcagcc
    13921 agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg cgtgaattgg
    13981 cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat cgttgaatgc
    14041 acaataaaaa aatcccgacc ctgagggggt cgggatgaaa cttgcttaag caagaagcac
    14101 ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatccg caattttttt
    14161 cgctgacaag aaaatattcg cgatatgaat aattaacgct acttatgaat agaaatgtga
    14221 cgtgcgtcac cccttggtgg ctctggcgtc aacgggatcg ctcccgaaaa aatcatcttt
    14281 gattatttgt gctgttttga gaatcatcct ggtaagggga gaactgcacg accatgtttt
    14341 caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg gacagtcata
    14401 gttatgactg gcaaccgact gccctctggc tcagccgttt acgcgaagcg aatgttcccg
    14461 tcattctctg tagcagtaaa acttcagcgg aaatgctgta cttgcaaaaa acgttggggc
    14521 tacaagggtt accactgatt gccgaaaatg gcgcagtgat ccagcttgct gaacaatggc
    14581 aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa atcagccagg
    14641 ttttaaatac gctacgcgag aaagaacatt ttaaattcac gactttcgat gatgtcgacg
    14701 atgcaaccat cgccgaatgg acgggattaa gccgtagcca ggcggcgctg acgcagctgc
    14761 atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca caatttaccg
    14821 ctcgtctgaa cgaactgggc ttacagttta tgcagggtgc gcgcttctgg cacgtcctgg
    14881 atgcctctgc cggaaaagat caggccgcca actggattat cgcgacctat caacaattat
    14941 caggcaaacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg cccttactgg
    15001 aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggtg catctgcatg
    15061 atgaggatcc gacccgcgtc tggcgaacgc agcgtgaagg accggaaggc tggcgtgaag
    15121 ggctggacca ttttttctcc gctcgttaag cgttatcgct cgcgcatacc cgattacgcc
    15181 cggcctgttt agcgagataa agccgacggt cagccagtga ctggagttgt tcaaaatcat
    15241 aatcaccggt ttcctcgctg ctacttaccc ccaacgaggc actgatgcgt atcgtcgtac
    15301 tcttagcaat caacatctct ttttcattta acttcaggcg aatacgttct gcgacttccg
    15361 cagcctccgt cagactcgcg cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc
    15421 cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga gaaagaacac
    15481 ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca aggtcgacct
    15541 gaatgacaga aaaaggatgt tggtgcgtct gacacaattt cgcgagcgga cgggcttttt
    15601 caaacagtgc gccacgatta tataaacggg ttaaggtgtc gtgccacgcc tgccactgca
    15661 acgagctttg cagaacatac atgttgctaa ccatccggcg aatcacatac caggagatga
    15721 gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata ctgccgaaat
    15781 cgccgcgcac gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg aaatggtcca
    15841 gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg tcatgttcca
    15901 tcgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt ggatggtcag
    15961 gattggaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca ccatcgaggt
    16021 ttttatcgat ggcgtttctt aaaaattgct gcatggtacg cacgggaata ctcatcccca
    16081 gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc tgcggttcag
    16141 tattgctggc gtgttccggt tgcgaagtaa accagcgtac cgcacggtga cgattttctc
    16201 gttgcgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc gttggtacat
    16261 tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc ggcacgcgag acatacatcg
    16321 cctgttcaac catcgacgag gtgttgtgcg ccagtcgcag caagtaacca acttccagtg
    16381 cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggagattc ccctcgctga
    16441 ctaatgcatc cgatacgcca ttgactgaca gggtgcgtcg tcggttgagt tcaatttgcc
    16501 aggcgtgctc gtcgcgatgc tgttcgaact cggttacagc attacgcagt gaagtgaaat
    16561 ccagtggcgc tacgagagct tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt
    16621 tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta cgctggctgg
    16681 agatataggc atcttccagc actaccactt cacgccaggt cagcaaggtg gaaaaaagca
    16741 ataccacgat aaagcagaga ttaacgacat gaccaggccc caggcggcgt gcgagttttt
    16801 tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact cccaacctgc
    16861 ttttttatga ttctggcata acctggcgat agcgtaccgc aaaacagcga taattttata
    16921 atggtggcat gataagacgc gactatcatt cattgcaggc attggcacac caggccggaa
    16981 gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac gactcccctc
    17041 ccccggaaca agctcggttt catgaaaagc ttcccgcttg accccgtaac cgtcatacca
    17101 acgacactca accataccgc tggagtatcc agtgacaatc atccgcgggc cgccctcttt
    17161 aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc gagtgaaact
    17221 tgttcacctt agttgaagat ggcgaatttt gcataatagc ctgtacgagt attaatgtgc
    17281 ctgaccgcgc attttcgcta cggctttcac tcccagcacc acgatgccgc cgatgataaa
    17341 tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac tttgctgccc
    17401 ggcgaaatgt tcaatggcgt gatgcagcgg cgcaatacca tgtaccacaa taccgccgcc
    17461 gacgaggaac atcgccagcg tgccgacaat cgataacgcc ttcatcaacc agggcgcaat
    17521 gatcaataac cctttaccta atgcctgcat cagcgcgctg gatttttccg ccagccaata
    17581 ccccaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca cggtcactac
    17641 cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcttcgg ccacaatccc
    17701 cagcgtgatg gcgacgattt cggcagacaa tataaaatcg gtacgaatcg ccccttttat
    17761 tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac gctgctggct
    17821 ctgcgccgga tcttctttat gtttacgcgc ctccagcata tgcagcactt tctctactcc
    17881 ttcaaagcag agaaacgcgc caccaatcat caacagaggc gtaatcgccc acgggataaa
    17941 cgcactgatg atcagcgcca gcggcaccag aatcacttta ttaatcagcg atcctttcgc
    18001 cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt gttgcgcatt
    18061 gagcgataag tcatccccta atacaccggc ggttttcttc gccgccagtt tgcccatcac
    18121 ggagatatcg tccagtagtg tggcgatatc atcgagcaac gtcagtaaac tactcccggc
    18181 caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca aaaacggcag
    18241 ctctgatact tgcttcacag gtcaacattt tttttacaat caagtgcaat tatatctctc
    18301 gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccac cgtgagggat
    18361 tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt atatcatttg
    18421 gggctcaacc tattttgtca ttcggattgg cgtggaaagc tggcctccgt taatgatggc
    18481 aggcgttcga ttcctggcag ccggtatttt attgatggca tttttgctac tgcgcggaca
    18541 caagctcccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt tattgctggc
    18601 tgtcggtaat ggcatggtga cggttgccga gcatcaaaat gttccttccg gcatcgccgc
    18661 cgttgtggtt gcaaccgtgc ccctctttac cctggtgctt cagccgcctg tttggcatta
    18721 aaacgcgcaa actggaatgg gtgggtatcg ccattgggct tgccggaatc atcatgctca
    18781 atagcggtgg aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga
    18841 ttagctgggc gtttggctcg gtttatggct cgcgcattac cttacctgta gggatgatgg
    18901 cgggtgcgat tgagatgctg gcggcaggcg tggtgttaat gatcgcgtcg atgattgcag
    18961 gtgaaaaact gacggcgctc ccttcccttt caggcttcct tgcggtcggc tatctggcgc
    19021 tgtttggttc gattatcgcc atcaacgctt atatgtattt aatccgtaat gtcagtccgg
    19081 ctctcgccac cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac
    19141 tgggcggaga gacgctgtcg aagattgaat ggctggcgct cggcgtaatt gtcttcgcgg
    19201 tggtactggt cacgttggga aaatatctct tcccggcaaa acccgtagtt gcgccagtta
    19261 ttcaggacgc atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct
    19321 tcgccgcaga tccactcttc cagacgctcg gtaagcgctt catccgtcag cttctcgcgc
    19381 ccacgtaatg cacactccca gacaatcagt acgcgccagc cgagttcctg caaacgactg
    19441 atatcgcggc gatcgcgttc aacattttta cctatcttct ccagccagaa ctcggttcga
    19501 gtcgcaggca ctttaaacag atagcagtga tgatgatgcc agaagcagcc atgggtaaat
    19561 atcacgcagc gatattcatc aacgacaaaa tccgggcttc cgggcagact ggcgtcctga
    19621 acgcgaaacg ccaggccctg cccggttaac agactggcga ggcgcttctc aatcgccgta
    19681 tcacgcgtgg caatcgcgcg catatttttg ctgcgagtgg ccttatcgtg aacgtcggcc
    19741 atgttgtgcc tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt
    19801 tgccaccgcg gcaaagaccg gcacgaccac cgagttaccg aactggcgat aggcctgagt
    19861 gtccgaaacc ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc
    19921 gcattcccga ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaaatc
    19981 tttctcaccc gtggccatat cccagccgcg atcgattaaa atttccgcgc catctttgta
    20041 ataacgcgca gacagcgtac gcgtgacgct ttgcggattg ttcggataaa ccattccata
    20101 accgaagccg ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag
    20161 caccggcgtc aggatatatt tcgcctcgac catcgggtcc aacagctgcg ccagcgtcac
    20221 tcgctgtgca gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattaag
    20281 atcgcgacga aaacccacca gcacgatgcg ttcacggtgc tgcggcagaa aatgtttacc
    20341 atcgatgatt ttcggatcgt ccggcccgtt atcttctgca tcagccacgt catagcccag
    20401 ttcgtccagc gtctgcatga tgatgcggaa cgttttaccc tggtcgtgac ttttcaggtt
    20461 tttgacgttt tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gtaccacatc
    20521 aaaaaacagc gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt
    20581 tttcgatacg ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg
    20641 ttcaggaatg tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttctttatg
    20701 gctgagggtg atgtcgcgga tatcttcatt aaaatgatgc gtcgccggat cgcaataatg
    20761 gttggcttta taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcattgtcc
    20821 gccaatcgat tcaaaaccgc gacggatgcc gccaattccg gcaaatagat cgataaagcg
    20881 aaacgcataa tgcggatgat gtgccgatgg tttgggtaac aacgtttgca gatgggcgaa
    20941 ctctttctca cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc
    21001 cgcgctccag tgattctcac ccacaccatt aagctgcgcc accagcgttt taacatcata
    21061 aatttgcagc agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt
    21121 tactgatata ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca
    21181 taattacagc catttagggc cacgcgctta tcccgcgggt gaaaacgcat ctatcacctt
    21241 gtgatcgaca ccttcattct cccccgccag ttcggcactg agcttcgcca taaactcgac
    21301 taaaaaatgc gcgttgtgct gcgccagctg cttgcctctt gcggtttgca tggtttgcgg
    21361 tagcttcagc aacttagtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg
    21421 atgctgtgcg aacggatctt caccatcaaa cagcgccacg cccaatgccc ctgaaacggc
    21481 aaacacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat
    21541 tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg
    21601 acaaacggcc tcgattttct ccgccggaaa ttgctcgaac tcttcacgga gcaggcgacg
    21661 cgtctcttct gccgccagga ttgaagaacg ctgcctttgc ggatgatttt tcgccaggct
    21721 cacaatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc
    21781 cgccagcttc tgtgccgcgt cctggtgctg atgatggttt ttcaaccagt tttcgaactg
    21841 tgcctgccag tgttgtaagt ccatacgcgc tccgttgcca aagagtcgcc accttagcag
    21901 tttttattca tagcgtctgc tacgtgcggg acgaggtcgg gtgtaccagg caatgctgcc
    21961 taacaatgcc ccgaccagag ccataaataa aaatccgacc agtgccccta accactttcc
    22021 cgcggtagag cctaaatcgc tttcaaatcc cgacaccggg gcatcaggga tccggctcat
    22081 cacccagatg taacgcgcca ttgcccagac gaaatagcca caccatgtat agaaagccca
    22141 caaagccaat ttgccgccag ggctgcgaga gagtctctct tccatctttg tcgaaccgta
    22201 ttccattttg agataacccg gaaaattcag acttagtact attatgtgat aaaagtcaca
    22261 tttttccaca ttggatgagc cagtcaagac tcaatcattt tttgaacaaa tagataaatt
    22321 ttgcgcaacg gcaataatta ctggaaaaac agcaataaag atttatttat atccttggta
    22381 atagttttat taattcaccc aaacaatata ttagcactga atacaattat accttttttg
    22441 atttctgtgt catgataacc ccgttattag ccttttatcg tcttgtttat attttttggg
    22501 ccggcatgat gccggctttt tttatacctt cattaatgtg cgcgtgatca cgcctgccgt
    22561 ttggcgcaac aatcattg
//
LOCUS       DAZOZD010000048        22309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOZD010000048 DAZOZD010000000
VERSION     DAZOZD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.048"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(110..3259)
                     /gene="acrB"
                     /locus_tag="ACHUPB_001491"
     CDS             complement(110..3259)
                     /gene="acrB"
                     /locus_tag="ACHUPB_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0758344.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3282..4475)
                     /gene="acrA"
                     /locus_tag="ACHUPB_001492"
     CDS             complement(3282..4475)
                     /gene="acrA"
                     /locus_tag="ACHUPB_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0758345.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4617..5264
                     /gene="acrR"
                     /locus_tag="ACHUPB_001493"
     CDS             4617..5264
                     /gene="acrR"
                     /locus_tag="ACHUPB_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0758346.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5392..8754
                     /gene="mscK"
                     /locus_tag="ACHUPB_001494"
     CDS             5392..8754
                     /gene="mscK"
                     /locus_tag="ACHUPB_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0758347.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8815..8913
                     /locus_tag="ACHUPB_001495"
                     /pseudo
     CDS             8815..8913
                     /locus_tag="ACHUPB_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8966..9127)
                     /gene="rsmS"
                     /locus_tag="ACHUPB_001496"
     CDS             complement(8966..9127)
                     /gene="rsmS"
                     /locus_tag="ACHUPB_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0758348.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(9141..9668)
                     /gene="priC"
                     /locus_tag="ACHUPB_001497"
     CDS             complement(9141..9668)
                     /gene="priC"
                     /locus_tag="ACHUPB_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0758349.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9738..10115
                     /locus_tag="ACHUPB_001498"
     CDS             9738..10115
                     /locus_tag="ACHUPB_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0758350.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10268..10819
                     /gene="apt"
                     /locus_tag="ACHUPB_001499"
     CDS             10268..10819
                     /gene="apt"
                     /locus_tag="ACHUPB_001499"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0758351.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10948..12873
                     /gene="dnaX"
                     /locus_tag="ACHUPB_001500"
     CDS             10948..12873
                     /gene="dnaX"
                     /locus_tag="ACHUPB_001500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0758352.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12206..12270
                     /gene="dnaX"
                     /locus_tag="ACHUPB_001500"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12926..13255
                     /locus_tag="ACHUPB_001501"
     CDS             12926..13255
                     /locus_tag="ACHUPB_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0758353.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13255..13860
                     /gene="recR"
                     /locus_tag="ACHUPB_001502"
     CDS             13255..13860
                     /gene="recR"
                     /locus_tag="ACHUPB_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0758354.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13970..15844
                     /gene="htpG"
                     /locus_tag="ACHUPB_001503"
     CDS             13970..15844
                     /gene="htpG"
                     /locus_tag="ACHUPB_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0758355.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16025..16669
                     /gene="adk"
                     /locus_tag="ACHUPB_001504"
     CDS             16025..16669
                     /gene="adk"
                     /locus_tag="ACHUPB_001504"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0758356.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16801..17763
                     /gene="hemH"
                     /locus_tag="ACHUPB_001505"
     CDS             16801..17763
                     /gene="hemH"
                     /locus_tag="ACHUPB_001505"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0758357.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17760..18719)
                     /gene="aes"
                     /locus_tag="ACHUPB_001506"
     CDS             complement(17760..18719)
                     /gene="aes"
                     /locus_tag="ACHUPB_001506"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR0758358.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18871..20175
                     /gene="gsk"
                     /locus_tag="ACHUPB_001507"
     CDS             18871..20175
                     /gene="gsk"
                     /locus_tag="ACHUPB_001507"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0758359.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20308..21984)
                     /gene="ybaL"
                     /locus_tag="ACHUPB_001508"
     CDS             complement(20308..21984)
                     /gene="ybaL"
                     /locus_tag="ACHUPB_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0758360.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22222..>22309)
                     /locus_tag="ACHUPB_001509"
     CDS             complement(22222..>22309)
                     /locus_tag="ACHUPB_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758361.1"
                     /translation="IELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5433 a         5915 c         5853 g         5108 t
ORIGIN      
        1 atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa tgttcgtagg
       61 ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat caatgatgat
      121 cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg cgaaccacca
      181 caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc ccgcccatta
      241 caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata accagcggca
      301 taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc atccgcactg
      361 catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct ttggcgaatt
      421 cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg cctacctgga
      481 agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg ataaccccca
      541 gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc gccgccagac
      601 acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg ccggagagac
      661 gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt ttgctcgcca
      721 gttgttccat cagctccatt gcttcaccgg tacttttacc cggtgccgcc tggcctaaga
      781 tttccatgga tggcaggccg ttgtaacgtt ccagacgcgg cgaaccgtac tcccaacgag
      841 aagaggagaa cgccgagaat ggcaccatct gaccatcagc agcacgaaca taccagtcgc
      901 cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact ttcttcacac
      961 gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc agagaggtgt
     1021 taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata tcaatcttaa
     1081 actgcggggt atcttccaga ccgtttggac gtacgctggt cagcatatca gggtgcttcg
     1141 ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca aggccagcct
     1201 ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt gcgggcaggt
     1261 taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca cgcatggtaa
     1321 tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag gaaacgaacg
     1381 caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg aacaccgact
     1441 caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc actttctgcg
     1501 tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg ccctggtcct
     1561 catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg accacgatga
     1621 tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg ctgtcggtgt
     1681 agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct tttttacctt
     1741 ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc ggagtcagga
     1801 tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag aactgacgat
     1861 agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgcc gacagtacca
     1921 tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta gcttcttttg
     1981 gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg atagcgtcat
     2041 ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt atcgagaagc
     2101 caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca atggtcggaa
     2161 tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg aggatgatcg
     2221 cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc gtggtgtcgt
     2281 acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc agttcagcac
     2341 ggattgccgc agcggtatcc agcgcgtttg caccggtcgc cagcttgatc cccagaccgg
     2401 aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc agctcaatct
     2461 tcgccacatc acgcagcagt acgcgggaac catcctgatt cactttcagc aggattttgc
     2521 cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta agctgttggc
     2581 ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc tgcgctttga
     2641 tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc gggttcatcc
     2701 agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc gacgtacggc
     2761 tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc gtcatggtgc
     2821 catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc tcaacgctca
     2881 ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt ttgttctgca
     2941 cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg atctgcacgg
     3001 tacccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg ttcatattct
     3061 gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg gggtaggagg
     3121 cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc agtttgagga
     3181 tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa atcgggcgat
     3241 cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt ggactgttca
     3301 ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg tgcttttacc
     3361 tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc tgctttcaga
     3421 ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg acgggtttcc
     3481 actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt acgggttacg
     3541 ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg tgcgcgcacg
     3601 aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag ggtgatagac
     3661 ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg cgggaactta
     3721 atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt cagcgtgcca
     3781 ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac atcaaggtag
     3841 atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg taccaatgcg
     3901 ccttccgtca cgttcgattt accaatgcga ccgctaatcg gagaggtgac tttggtgtaa
     3961 gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc agcattcgcc
     4021 tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg agtaccgagc
     4081 agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg ggctttcgcc
     4141 agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat ctgatagaga
     4201 gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat aatcccgcta
     4261 acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag ctcggttgtg
     4321 atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg ctggccacct
     4381 tgttgggcct gtttgtcgtc acatcctgtt agggctaagc tgcctgagag catcagaacg
     4441 accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt gtccgatttc
     4501 aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta tcgtcgtgct
     4561 atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac gaacatatgg
     4621 cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat gtggctctac
     4681 gtcttttctc acagcagggg gtatcatcca cctcgctggg cgagattgca aaagcagctg
     4741 gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg ttcagtgaga
     4801 tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag gcaaaattcc
     4861 ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa tccacggtga
     4921 cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa tttgtcggag
     4981 aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat gaccgtatag
     5041 aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta atgacgcgtc
     5101 gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg ctctttgccc
     5161 cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta ctggagatgt
     5221 atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga atctgactcc
     5281 aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg tgatattctt
     5341 gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat catgactatg
     5401 ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc ttttgttttt
     5461 gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga tctgccgaca
     5521 aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct ttctgctcag
     5581 gacaaactgg tgcagcagga tctgacagat acattagcca ccctcgataa aatcgatcgc
     5641 ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga aaaaatgcgc
     5701 caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga aacgcgcaaa
     5761 attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc gctggacgat
     5821 ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc gttacagacg
     5881 cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca acaaattcgc
     5941 agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca gaaagtgtta
     6001 atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa aagcctggaa
     6061 gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac ggcgaacagc
     6121 gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa gcgcctgact
     6181 ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg tattcaggct
     6241 aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg tctgattacc
     6301 gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa ctggctggag
     6361 cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa gggcagcctg
     6421 ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga tgaactggaa
     6481 aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa ccagcagcgt
     6541 gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca caccaacgaa
     6601 gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg cgaattgctg
     6661 gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct gcaaatcaac
     6721 cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca gcaaatcttt
     6781 tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc gcaaagcctg
     6841 aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg gcctgccgtt
     6901 tttatcgctt tcctcgccgg tttgccgctg ctgttgattg ccgggctgat ccactggcgt
     6961 ctgggctggc tgaaagcgta tcaacagaaa ctggcttccg ctgtgggttc cctgcgtaac
     7021 gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc gctgccggtg
     7081 tgcctgatta ttctcgcggt tggcctgatt ctgttgacca tgcagctcaa catcagcgaa
     7141 ctgctatggt cgttcagtaa aaaactggcg attttctggc tggtgtttgg cctgtgctgg
     7201 aaggtactgg agaaaaatgg cgttgccgtg cgtcacttcg gcatgccgga gcagcagacc
     7261 agccactggc gtcggcaaat tgtccgcatc agtctcgcgt tgctgcctat ccatttctgg
     7321 tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg gcaggcgatg
     7381 attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg cgaaagctgg
     7441 cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc gattatcccg
     7501 attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg cctctccggg
     7561 cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca gacggtattg
     7621 cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc gcgtcggcag
     7681 aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac cattgcactg
     7741 gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc gctgttcggt
     7801 gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatct cgacagcatc
     7861 acgctctggc attacaacgg cactgaagct ggcgctgcgg tggtgaaaaa cgtcaccatg
     7921 ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat tcgcaacctg
     7981 cctggtttac tggaagtgct ggtgctctcg cgactgaata tgcgtcaggg cgcgtcgtat
     8041 gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac ggtgttcgga
     8101 tcgctgggcg tctcttggga taaactccag tggctggccg cagcattatc cgtaggtctt
     8161 ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat tctgttcgaa
     8221 cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac ggtaagtaag
     8281 atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat catcccgaac
     8341 aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac tacgcgtctg
     8401 gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa agtgttactg
     8461 aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt cttctttacg
     8521 gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga actgcgtgac
     8581 cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg tgaaaacgac
     8641 atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg cgatgaggtg
     8701 acggaagtca aacgcgacta caaaggcgat gacccgacgc cagcggtagg gtaataacca
     8761 aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt tcatgccgga
     8821 tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta ttgcaggagc
     8881 ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac aatcttaggg
     8941 gacatttagc ggccccattt atttctcact tttccgcctt atcatcgcgc gttaatttct
     9001 tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct gggatctgat
     9061 tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg gcattttcaa
     9121 gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc gattttttcc
     9181 agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg atgcagcgtt
     9241 tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc aaccatctca
     9301 cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg ggcaattttc
     9361 ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc gatggcttcc
     9421 agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg ctgctcaacc
     9481 gcatgacaaa gcgcagccag attatcgccc gcctcgtcga gacaagcctg tagtgttgtc
     9541 gcacgagtct gaaaaagatg cctgtcgaaa cgagcactta gcgtggcgaa ctgtgacacc
     9601 ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag cagcagggcg
     9661 gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag ccactctgta
     9721 agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg ctggcggtag
     9781 tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt atcctgctgg
     9841 cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg taccgctcat
     9901 ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc ggcgtcaaac
     9961 cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc gtccagatgc
    10021 catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat atgtggcgaa
    10081 ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca gttgcaatta
    10141 ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt caaaggttaa
    10201 acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa caagcaggca
    10261 tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca tcaaaagcat
    10321 tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac tggaagaccc
    10381 gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg cgggcattac
    10441 caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag ctctgggtct
    10501 gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa ccatcagtga
    10561 aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg ccatcaaacc
    10621 gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta tcgaagcgac
    10681 cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca ttatcaacct
    10741 gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct acagccttgt
    10801 cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg gacagcacaa
    10861 gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc ttcgtgaatc
    10921 caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg aaaatggcgc
    10981 ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact ggcgaacggc
    11041 ttgtcgttag ggcgtattca tcatgcttat cttttttccg gcacccgtgg cgtcggaaaa
    11101 acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat taccgcgacg
    11161 ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt cgatctgatt
    11221 gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct ggataacgtc
    11281 cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt gcatatgctg
    11341 tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga gcacgttaag
    11401 tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc gcgctgtttg
    11461 caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga gcacatcctc
    11521 aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg cgccgctgaa
    11581 ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg tgacggccag
    11641 gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg atgacgatca ggcgctgtcg
    11701 ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat taatgaagcc
    11761 gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct gttgcatcgt
    11821 attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc cattgagctg
    11881 cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta ctatcagacg
    11941 ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg cgttgagatg
    12001 acgctgctgc gcgcgctggc gttccatccg cgtatgccgc tgcctgagcc agaagtgcca
    12061 cgccagtcct ttgcacccgt cgtgccaacg gcagtaatga cgccaaccca ggtgccgccg
    12121 caatcagcgc cgcagcaggc accgactgta ccgctcccgg aaaccaccag ccaggtgctg
    12181 gcggcgcgcc agcagttgca gtgcgtgcag ggagcaacca aagcaaaaaa gagtgaatcg
    12241 gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc tggaaagact ggcttcggtc
    12301 accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg aaaaagcgtc agccaaaaaa
    12361 gaagcgtatc gctggaaggc gaccactccg gtgatgcagc aaaaagaagt ggtcgccacg
    12421 ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc cggaactggc ggcgaagcta
    12481 gcggcagaag ccattgagcg cgacccgtgg gcggcacagg tgagccaact ttcgctacca
    12541 aaactggtcg aacaggtggc gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt
    12601 ctgcatttgc gctcctctca gcggcatttg aacaaccgcg gtgcacagca aaaactggct
    12661 gaagcgttga gcatgttaaa aggttcaacg gttgaactga ctatcgttga agatgataat
    12721 cccgcggtgc gtacgccgct ggagtggcgt caggcgatat acgaagaaaa acttgcgcag
    12781 gcgcgcgagt ccattattgc ggataataat attcagaccc tgcgtcggtt tttcgatgcg
    12841 gagctggatg aagaaagtat ccgccccatt tgatcgtaag cacagcttac gttcgtcatc
    12901 cttaacgtga ttgagagaga aacctatgtt tggtaaaggc ggtctgggta acctgatgaa
    12961 gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga
    13021 agtcaccggc gaatctggcg caggtctggt aaaagtgacc atcaacggtg cacacaactg
    13081 ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac aaagagatgc tggaagacct
    13141 ggtggctgca gcattcaacg acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat
    13201 ggcctctgta tcctccggaa tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa
    13261 accagcccgc tgttaacaca gcttatggaa gcactgcgct gtctgccggg cgttggcccg
    13321 aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt
    13381 ttggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc actgcgccga ttgccgcact
    13441 ttcaccgaac aggaagtctg taacatctgt tcgaatccgc gtcgtcagga aaacggtcaa
    13501 atctgtgtgg tggagagtcc ggcggacatc tacgccattg agcagacggg gcagttttct
    13561 ggtcgttatt ttgtgttgat ggggcatctg tcaccgctgg acggcatcgg tccggatgat
    13621 atcgggcttg atcgtctgga acagcgtctg gcagaggaaa aaatcactga agtgatcctc
    13681 gccaccaatc ccacggttga aggtgaagct accgctaact acattgccga gctttgcgcg
    13741 caatatgacg tggaagccag ccgaatcgct catggcgtac cggttggcgg cgaactggaa
    13801 atggtcgatg gcaccacgct gtcacactcc cttgccgggc gtcataagat tcgtttttaa
    13861 gcaaacgaga gcaggatcac ctgctctcgc ttgaaattat tctcccttgt ccccatctct
    13921 ccctcatcct gtttttaacc ttaaaatggc attattgagg tagacctaca tgaaaggaca
    13981 agaaactcgt ggttttcagt cagaagtgaa acagcttctg cacctgatga tccattctct
    14041 ctattccaat aaagaaatct tcctgcgtga gcttatctct aacgcctccg atgcggcgga
    14101 caagctgcgt ttccgtgcgc tatctaaccc ggacctgtac gaaggtgatg gcgaactgcg
    14161 cgttcgtgtc tctttcgata aagacaagcg cacgctgacc atctctgata acggcgtggg
    14221 gatgacccgc gacgaagtga ttgaccatct ggggactatc gctaaatccg gtaccaaatc
    14281 attcctcgaa tccctgggtt ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg
    14341 tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg accgtgcgta ctcgcgcggc
    14401 aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct ggcgaaggtg aatataccgt
    14461 tgccgacatc accaaagaag atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga
    14521 agacgagttc ctcgatgact ggcgcgtgcg ttccatcatc agcaaatact ccgaccatat
    14581 cgcgctgccg gtagagatcg aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg
    14641 ggagaaaatc aacaaagcgc aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga
    14701 agagtacaaa gagttctaca aacacatcgc ccacgacttt aatgatccgc tgacctggag
    14761 ccacaaccgc gttgaaggta agcaggagta caccagcctg ctgtacattc cgtcccaggc
    14821 tccgtgggat atgtggaacc gtgatcataa acacggcctg aaactgtacg tccagcgtgt
    14881 gttcatcatg gacgacgcag aacagttcat gccgaactat ctgcgcttcg tgcgtggtct
    14941 gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa atcctccagg acagcacggt
    15001 aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc
    15061 gaaagacgac gcggaaaaat accagacctt ctggcaacag tttggcctgg tactgaaaga
    15121 aggtccggcg gaagatttcg ctaaccagga agcgatcgcc aaactgctgc gttttgcttc
    15181 tacccatacc gattcttctg cgcagaccgt atctctggaa gactacgttt cccgcatgaa
    15241 agaagggcag gagaaaatct actacatcac cgcagacagc tatgcggcag cgaagagcag
    15301 cccgcacctg gaactgctgc gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat
    15361 cgatgagtgg atgatgaact atctgactga gttcgacggt aaaccgttcc agtcggtgtc
    15421 taaagttgac gagtcgctgg aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc
    15481 ggagaaagca ctgactccgt tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa
    15541 agatgtccgt ctgactcacc gtctgaccga tacgccagcg atcgtttcga ccgacgcgga
    15601 cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt
    15661 gaaatacatc ttcgaactga acccggatca cgtactggtg aaacgtgcgg cagatactga
    15721 agatgaagcg aagttcagcg agtgggtaga actgctgctg gatcaggcgc tgctggcaga
    15781 acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc
    15841 ctgatgtcat gccggatgac ctttgtgtca tccggcattt ttcttttcat catctgcact
    15901 ttccgcaaat tatctcgcca ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg
    15961 cctgttggtg gtatcgttta tcgctttttc aaaaaaatcg acacatttta aggggatttt
    16021 cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg aaagggactc aggctcagtt
    16081 catcatggag aaatatggta ttccgcaaat ctccactggc gatatgctgc gtgctgcggt
    16141 caaatctggc tccgagctgg gtaaacaagc aaaagacatt atggatgctg gcaaactggt
    16201 caccgacgaa ctggtgatcg cgctggttaa agagcgcatt gctcaggaag actgccgtaa
    16261 tggtttcctg ttggacggct tcccgcgtac cattccgcag gcagacgcga tgaaagaagc
    16321 gggcatcaat gttgattacg ttctggaatt cgacgtaccg gacgaactga ttgttgatcg
    16381 tatcgtaggc cgccgcgttc atgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc
    16441 gccgaaagta gaaggcaaag acgacgttac cggtgaagaa ctgactaccc gtaaagacga
    16501 tcaggaagaa accgtgcgta aacgtctggt tgaataccat cagatgactg caccgctgat
    16561 cggctactac ttcaaagaag cggaagcggg taacaccaaa tacgcgaaag ttgacggcac
    16621 caagtcggtt gctgaagttc gcgctgatct ggaaaaaatc ctcggctaat caagccgcag
    16681 cagatctgct ttcgggcagg tctgctcatc cacctcaggc aatacctctc acagcaatta
    16741 gttcttcttc ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta
    16801 atgcgtcaga ctaaaatcgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca
    16861 cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc
    16921 tcacggttgt tatggtggcc attgctgcgc ggcgttattt tgccgctgcg ctcgccgcgt
    16981 gtggcgaagc tgtacgcctc ggtctggatg gaaggtggct cgccgctgat ggtttacagc
    17041 cgccagcaac agcaggcgct ggcacaacgt ttaccggaga cgcccgtagc gctgggaatg
    17101 agctacggct cgccatcact ggaaagcgcc gtcgatgaac tcctggcaga gcatgtagat
    17161 catattgtgg tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg
    17221 gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt
    17281 gattacgccg ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt
    17341 gccaaacatg gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat
    17401 gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa cgactcgcga actggcttcc
    17461 gcactgggga tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa
    17521 ccctggctga tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt
    17581 catatacagg tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt
    17641 gccgagcaaa accgtgaggt cttcctcggt gcgggcggga aaaaatatga atatattcca
    17701 gcgcttaatg ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc
    17761 taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc
    17821 atccgtgaat aatgcaaaaa ggcatgcagc gtacctgggt agagtttgaa ctcacagggc
    17881 tgctgatgcg ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac
    17941 tccgccccgg caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag
    18001 taatacggtg actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa
    18061 tcctgttgcg ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt
    18121 aatccgtaaa gcccatacca cagcaaaaca cccgcaattt taccgcaatc gatctgttta
    18181 tcacgcaacc acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag
    18241 ccaatgcggg acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca
    18301 gccacaattt cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggt gtaatcaata
    18361 ccaatcaccg tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg
    18421 agattgccga gaataaaacc gcctccatgc aaataaaata gcgtcgccgg gctatctggc
    18481 tgcggacaaa agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg
    18541 gttgccattt ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt
    18601 tgctcagcaa tcgttcccgt tgcgggccag gacggtaaat ccggctgaag agtattcaca
    18661 acggtcttca tttcagcaga aataaggtcc agaacaggta gtttgttttc cggcttcata
    18721 caaaaactcc tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg
    18781 cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg
    18841 ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa
    18901 cattacttcc ctgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa
    18961 accagcgccg cctgggtcgt aggtattgac cagacgctgg tcgatattga agcgaaagtg
    19021 gatgatgaat tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat
    19081 gacgtagccg aagcgcttta tcaggagcta aaacagaaaa acctgattac ccatcagttt
    19141 gcgggtggca ccattggtaa caccatgcac aactactcgg tgctcgcgga cgaccgttcg
    19201 gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca gttatgccta tcgttacctg
    19261 tgtaacactt ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt
    19321 cgttgcttta cgctgattgg cgagtccggg gaacgtacct ttgctatcag tccaggccac
    19381 atgaaccagc tgcgggctga aagcattccg gaagatgtga ttgccggagc ctcggcactg
    19441 gttctcacct cttatctggt gcgttgcaaa ccgggtgaac ccatgccgga agcaaccatg
    19501 aaagccattg agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggcaccaag
    19561 tttgtcattg ccgagaatcc gcagtggtgg cagcaattcc tcaaagatca cgtctctatc
    19621 cttgcgatga acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca
    19681 tctgacaagg cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg
    19741 tatatggcgg gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg
    19801 ggcgctatag cggaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc
    19861 cagaatccgc tgcgtgtcta ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc
    19921 atgaacacta atggagcggg ggatggcgca ttggcagcgt tgctgcatga cattaccgcc
    19981 aacagctacc atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact
    20041 tattcatcgt tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac
    20101 cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct
    20161 tactgggatc gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct
    20221 ggcatttggc tctcgatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt
    20281 tcatatttta catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt
    20341 ttccagcagt tccagcatag tacgggcaat ttcacgttca cccatcacta cctgattcgc
    20401 gccacgttcg gtgatatacg tcacttcatc gtcataatgg gcgcgggcga taatctcaat
    20461 atccgggttt ttcgcgcggg cagacgccac aatctcaccc gcttcataac cgttggggat
    20521 cgtcaggatc agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc
    20581 tgcattgccc aatacagcgc ggaccccacg ctctcgcagc tcatcaacac gggttcgtga
    20641 cgtctcaatc accaccagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc
    20701 tacacgaccg taacccacca atagcgcatg gttgcaaata tccactggga tctgtttctc
    20761 ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt
    20821 ctccagtagt gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac
    20881 caggttttgt ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa
    20941 cgcgaactca ccaatctgcg ccaggctggc ggcgatggtt aatgccgtac gttgggagtg
    21001 accaaacaga cggacaagga acagggccgc gaccgattta ccaaacagaa taattgccag
    21061 cgtcgccagc actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac
    21121 ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg
    21181 gtgactcagt tcagactcgt tcagtaccat cccggcaaag aacgcgccaa gtgcgaagga
    21241 gacgtcaaac aactccaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag
    21301 ggtaaatagc tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg
    21361 gcgacctacc agcatcataa tggcgataaa tgcgatcact ttgccgatgg tgatccccat
    21421 atcgactgca agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg
    21481 cagcaacacc agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat
    21541 ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt
    21601 actggcggtg gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac
    21661 ggcagagagc gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat
    21721 ggcgatggcc tttaccgcca tcaaatcctt cagcgaaaag tgcaaaccga cgccaaacat
    21781 caacagaatg acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc
    21841 cggagtgaat ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg
    21901 tagtttattg gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt
    21961 gataagcggg gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt
    22021 ggccgggaaa accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg
    22081 aataattatt gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa
    22141 acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt
    22201 ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa
    22261 catccccaat agtggcagga aagcacagat tttatagact aactcgatg
//
LOCUS       DAZOZD010000049        21950 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOZD010000049 DAZOZD010000000
VERSION     DAZOZD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.049"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            54..527
                     /gene="dfrA1"
                     /locus_tag="ACHUPB_001510"
     CDS             54..527
                     /gene="dfrA1"
                     /locus_tag="ACHUPB_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0758362.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            622..1146
                     /gene="sat2"
                     /locus_tag="ACHUPB_001511"
     CDS             622..1146
                     /gene="sat2"
                     /locus_tag="ACHUPB_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0758363.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1215..1712
                     /locus_tag="ACHUPB_001512"
     CDS             1215..1712
                     /locus_tag="ACHUPB_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758364.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1773..2144
                     /locus_tag="ACHUPB_001513"
     CDS             1773..2144
                     /locus_tag="ACHUPB_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758365.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2554..2931
                     /locus_tag="ACHUPB_001514"
     CDS             2554..2931
                     /locus_tag="ACHUPB_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758366.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            2954..3106
                     /locus_tag="ACHUPB_001515"
                     /pseudo
     CDS             2954..3106
                     /locus_tag="ACHUPB_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3162..4778)
                     /locus_tag="ACHUPB_001516"
     CDS             complement(3162..4778)
                     /locus_tag="ACHUPB_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0758367.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(4779..6305)
                     /locus_tag="ACHUPB_001517"
     CDS             complement(4779..6305)
                     /locus_tag="ACHUPB_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR0758368.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(6308..7975)
                     /locus_tag="ACHUPB_001518"
     CDS             complement(6308..7975)
                     /locus_tag="ACHUPB_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0758369.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(7972..10080)
                     /locus_tag="ACHUPB_001519"
     CDS             complement(7972..10080)
                     /locus_tag="ACHUPB_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0758370.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10067..10888)
                     /locus_tag="ACHUPB_001520"
     CDS             complement(10067..10888)
                     /locus_tag="ACHUPB_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0758371.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11048..12877)
                     /gene="glmS"
                     /locus_tag="ACHUPB_001521"
     CDS             complement(11048..12877)
                     /gene="glmS"
                     /locus_tag="ACHUPB_001521"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0758372.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(13039..14409)
                     /gene="glmU"
                     /locus_tag="ACHUPB_001522"
     CDS             complement(13039..14409)
                     /gene="glmU"
                     /locus_tag="ACHUPB_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0758373.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(14760..15179)
                     /locus_tag="ACHUPB_001523"
     CDS             complement(14760..15179)
                     /locus_tag="ACHUPB_001523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0758374.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(15200..16582)
                     /gene="atpD"
                     /locus_tag="ACHUPB_001524"
     CDS             complement(15200..16582)
                     /gene="atpD"
                     /locus_tag="ACHUPB_001524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0758375.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(16609..17472)
                     /gene="atpG"
                     /locus_tag="ACHUPB_001525"
     CDS             complement(16609..17472)
                     /gene="atpG"
                     /locus_tag="ACHUPB_001525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0758376.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(17523..19064)
                     /gene="atpA"
                     /locus_tag="ACHUPB_001526"
     CDS             complement(17523..19064)
                     /gene="atpA"
                     /locus_tag="ACHUPB_001526"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0758377.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(19077..19610)
                     /gene="atpH"
                     /locus_tag="ACHUPB_001527"
     CDS             complement(19077..19610)
                     /gene="atpH"
                     /locus_tag="ACHUPB_001527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0758378.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(19625..20095)
                     /gene="atpF"
                     /locus_tag="ACHUPB_001528"
     CDS             complement(19625..20095)
                     /gene="atpF"
                     /locus_tag="ACHUPB_001528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0758379.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(20157..20396)
                     /gene="atpE"
                     /locus_tag="ACHUPB_001529"
     CDS             complement(20157..20396)
                     /gene="atpE"
                     /locus_tag="ACHUPB_001529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0758380.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(20443..21258)
                     /gene="atpB"
                     /locus_tag="ACHUPB_001530"
     CDS             complement(20443..21258)
                     /gene="atpB"
                     /locus_tag="ACHUPB_001530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0758381.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(21267..21647)
                     /gene="atpI"
                     /locus_tag="ACHUPB_001531"
     CDS             complement(21267..21647)
                     /gene="atpI"
                     /locus_tag="ACHUPB_001531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0758382.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         5650 a         5653 c         4952 g         5695 t
ORIGIN      
        1 ggtgacaaaa cgagcatgct tactaataaa atgttaacct ctgaggaaga attgtgaaac
       61 tatcactaat ggtagctata tcgaagaatg gagttatcgg gaatggccct gatattccat
      121 ggagtgccaa aggtgaacag ctcctgttta aagctattac ctataaccaa tggctgttgg
      181 ttggacgcaa gacttttgaa tcaatgggag cattacccaa ccgaaagtat gcggtcgtaa
      241 cacgttcaag ttttacatct gacaatgaga acgtattgat ctttccatca attaaagatg
      301 ctttaaccaa cctaaagaaa ataacggatc atgtcattgt ttcaggtggt ggggagatat
      361 acaaaagcct gatcgatcaa gtagatacac tacatatatc tagaatagac atcgagccgg
      421 aaggtgatgt ttactttcct gaaatcccca gcaattttag gccagttttt acccaagact
      481 tcgcctctaa cataaattat agttaccaaa tctggcaaaa gggttaacaa gtggcagcaa
      541 cggattcgca aacctgtcac gccttttgta ccaaaagccg cgccaggttt gcgatccgct
      601 gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc ctgagcaggt ggcggaaaca
      661 ttggatgctg agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa
      721 ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat
      781 gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag
      841 attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac
      901 acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta
      961 agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc tgcctgcaat
     1021 ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tgttcacgta taaaactaga
     1081 cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc acaggatgac
     1141 gcctaacaat tcattcaagc cgacaccgct tcgcggcgcg gcttaattca ggagttagag
     1201 agctggggaa gactatgcgc gatctgttga aggtggttct aagcctcgta cttgcgatgg
     1261 catcggggca ggcacttgct gacctgccaa ttgttttagt ggatgaagct cgtcttccct
     1321 atgactactc cccatccaac tacgacattt ctccaagcaa ctacgacaac tccataagca
     1381 attacgacaa tagtccatca aattacgaca actctgagag caactacgat aatagttcat
     1441 ccaattacga caatagtcgc aacggaaatc gtaggcttat atatagcgca aatgggtctc
     1501 gcactttcgc cggctactac gtcattgcca acaatgggac aacgaacttc ttttccacat
     1561 ctggcaaaag gatgttctac accccaaaag gggggcgcgg cgtctatggc ggcaaagatg
     1621 ggagcttctg cggggcattg gtcgtcataa atggccaatt ttcgcttgcc ctgacagata
     1681 acggcctgaa gatcatgtat ctaagcaact agcctgctct ctaataaaat gttaggcctc
     1741 aacatctagt cgcaagctga ggggaaccac tagtgtcata cgaacctcca agagacggtt
     1801 acacaaacgg gtacattgtt gatgtcatgt atgacaatcg cccaagtaag tatccagctg
     1861 tgttcagaac gtacgtccga attccagact atgtcgaact agataacgcg gaggagctgg
     1921 ccgacgcaat cattcgagag gttgagttaa aggaggcatg gctcacaagg gtcttgggga
     1981 actcagccga tcctgacgct tctgtgggca agtgggaccg gcgcctagcg cagtgcggtg
     2041 gtattgatga gatgtcaagt ggggtgcagg caacatactt cagtccaaac ggtcagctgg
     2101 tcggcttagt ggcatacatt gatgccgaat ggatggatga atagctctga cgttacgatt
     2161 caggtgagac aaccagatgg gcattgcgta caagctcagt tgccaccgat gtggctacga
     2221 gtctgatctt ctttacctag gccaaggtat ggcgatgctt cctgagcaaa tagtcggaac
     2281 gtgcacatgc tgcgacaatc taacgacgat ttctataaat gaaagctacg gagtatgctc
     2341 actttgtggg gctaaggatc gtgtgatatt tacaagctat cagcatgagg caaggccaag
     2401 aaatccctgc tatctagaga gaagtttcaa atatcaatgc ccaagatgcc atgccttcag
     2461 tatggagcct ccggatctac cggagatgct ttgggactaa tgctccgctt tgcctcgggt
     2521 atccgttcga cctatgttta agaggaaacg cacgtgtctg ccgccaaagc tcttgcctat
     2581 gcactttgtg acaagttgga aagcaaaaaa gttgatgaag tcgtacgaaa tcttgtttct
     2641 agcggctgga atattaggga agtggcctgg gaagcttcgt cctttgatca tgaaatgccc
     2701 tactttgaca cggggcggga cttcgatatt ggatttcctg acacgctgtc atccaagatt
     2761 gagtttttga ccgcgaatgg atcaatcgtc caagcacgtg ctcgcctgca cttcaagcag
     2821 gctctaattt ttgccaaggc gtccaaacat gtcgccgcgc tcaagaactc attggattac
     2881 tactatggat cgtctgtaat cccccctcaa aaccacggag gctatttgta gaaaattcca
     2941 ttaatatgaa ctcaaggtgt tccctacgga cgaatcagcg atgaaggtgg tgtacctggc
     3001 tatccaggcg gcgaccaaga aatggacgat gccgatccgc aactggaagc cggccatgaa
     3061 ccgttttatg atcgaatttg gtgaccgttt aaatggccac ctttaactga ccggcaaaag
     3121 cacttacaca aaagggttta caggctcttt cagtcattcc tttaatgcgt aaattgctct
     3181 ctaaccactc tctcagccca cccttccata tcctctctcg atacttgcag ctcccccaac
     3241 ccctttggat gattcacccc tctatgcccg tcttgtccat ataattgatc aaacaaacta
     3301 tttggccaat tcaatgagct cttcaccact ccacgccgta tcttttcaaa atcattccgc
     3361 catgtttcgc tatcaacgcc actcaacact ttggtagaaa gcattttcac cccatccgat
     3421 gcatccacct ctagcaatac aaattcactg ccattacgtt gcaccccaac ggctttaatc
     3481 actcttggtg agccatcttt acacagatgt aatcggctac gcccaacttt tggcaaaacc
     3541 aaggtttctt caaaaagcac ccgacatgca aatttagttt ttagaattga aagtagctca
     3601 tcaaaagcag caaatcgatt agcaaaaata gagttgtaat tggttgcatc ctgcttccca
     3661 cccacatctg ccgctgctag gacaccacct aagtgtggct catcagtact aaccaaatta
     3721 ctattttcac ttgttgttgc ttcttctttt tctaaaatag tttggctact ttttcttgcc
     3781 ctgcgagatt tttcaattcg actaggtcta ctaaaactta tccatgtcgg ctcagcctct
     3841 atgactcgtg tgtctgtgtc tatattggct gtttcctcat cgtccaactg tagatgctca
     3901 tcatcaacaa ctgactctgt tgaaaccgct atgtgctgcg tactaccatc acctgcctcc
     3961 ttttcctgaa aagaggcatg gctaatagct gttgtgctag gaagcatagc gttgatttct
     4021 aaacctacta tttcctcaac taaataatcc ttatcctcgt tagaagaacg tccttttaca
     4081 tgtaatttcc aaccctgcat gggcggaggg tcaaagctaa agcaccagct ttcaacgccg
     4141 ttcttaattt ctctattttg ttgatagtgc ctaaaaatac tttcatacga atccataaca
     4201 tcttgatccg aaaacaacca aaccaaaagc tgcactacgg ccgactgctc taacgcccct
     4261 ttaggaaacg atgaactggg taagatcctt atctctaaat gatctcgctc aacctcatac
     4321 tgaacgtcga actcttgctg taaagcggta ctgctcaaac agcttcgaca gaaataggag
     4381 ttaattaaaa ataatgaccg agctagctct aactgtggga tgtgataacg agtcttgcta
     4441 tatggtgttt cagcttcgta aataaatgaa ctctgcgctc catccttgtt gcgtacggaa
     4501 gcaaaggacg agagattagg aaaatcgatt attttggctc gttgaagatc cgatgcttca
     4561 aactcaatca cacgatccgc cttatttatt tgctttgttg tactgttaac caccttagct
     4621 cgacttaata aaggcaaatg agtaaatcga gtccatttcc gttcttgcat tggattaaac
     4681 caaacaaaaa tacgccactc cttatgaccc gagttacgga ataaatgacc aatatgtaca
     4741 acctgcacat tgtcattaaa tgtagctagc ctaaccactc actcttcccc ataaaccatt
     4801 tccaagaatc tttgtgcttc ctcagttatc cgctctttag ataacgttgc acttcttaat
     4861 aatcgccagc gcctaagctc aacatcttcc tgtttaagct taataaaagc ttggctaatc
     4921 cgtctaattt gataatcttc cacactctct gagtaacgct ttaagcaaag cgctaccaaa
     4981 ggcagtttct gtagattttt tgcaagagag gttccgttag gagtttgctt taacagccag
     5041 ctcgatgtcg ctcttgggtg atcaaggcta ctatctagac gctttatgat tcttaatagt
     5101 tgtcgtacag caattcgatc tctttggttc caatcaactc taggggcggg tgccagacgc
     5161 tcttgttgat gctgttgatt ccagtgaact agccaatccc tgtcatgtcg gtaaagccaa
     5221 gcgtatagca ccccaccctc taaagactgt cttgccgcct taattccttg gtatttatgc
     5281 actagttgct gccagtcttt atgcttaaca gataaatctt cagagttagg ttgcacagac
     5341 tggctaacag gtcttgtcgt tatagagtgc tcagtaagag cacttgcctg ctgtagcgct
     5401 tctataaccg ttagttttgg caataaggct tgccacacaa tactatgctg taaataacta
     5461 aaggcttttc tatgcttacg gaatatactt ttcagccaac acgtgtcctt gttctctgcc
     5521 aactttaaat ccagtttctc tagtgcctca tcactaaaag tctgcctcac tctctccgcc
     5581 accaagtcat gacgaatgtg cttgcttttg gttagcccta gatcctgcgc taagcgctga
     5641 taaaacagcg tccactgctc aaggcttggg gaaagctctt gcgctcgtgg agcatctaac
     5701 agaggggcta tataagcagc tagtgctgtt aattgagata gggagtcttt ggggtagtct
     5761 gaaagcagct cagtatgacc caaagcccaa aattgatgtc ggtgatcatc tacagctcta
     5821 tcaaagaaga ctaaagcacc gtgttttgga caatatggca aagcgggcaa ataccaatct
     5881 cgttgccaaa aggcttcccc atacctattt agctgaagag caacgcaatc agggcagtat
     5941 ctaaagcggt tatcgctctt aactctagaa gcagcgactc ctagcattaa atgcaccgca
     6001 ccttgcgctt ggtactccat taaccgaata gcttcgtctc ggcgctcctt gcctacaaac
     6061 ggagcatata aagggaataa ggtatgctca taaataagct gctgaacagc gtaacgtcct
     6121 gtttgatgta gatgtcttgc tatcacacct aaatgcgagg gcagacctaa ggtagcgacc
     6181 accttgcggt tgccatacac ctcatccaac agctgcttag gactaacaat cccttgataa
     6241 acgcctgccc gtgcaatagt gctataaatc agctcattcg agtacggaac aggaaagttt
     6301 ctcatggcta acctgcttgt ttaaataagc tcgccatatc cactattacc cctttccctt
     6361 ttaaccgttc atgcatggtt ttttcaggtt ggcgttgtga atagatataa cgtaaatccg
     6421 tatcaggcaa gctatcccag tcgctgggct tgactacctt tatagccgaa acctttttac
     6481 tcttggaggg cttttctagt tctgacacta ccgtttcgcc ttccatcaac cactgcaaaa
     6541 caagaggcag taacttttgt cttgtcatcg ttggattctg gctaaacgct tttttaatag
     6601 tgggaattaa caggcttgaa tcgtaatcct ctttcagcat cagatataaa tgacgctgat
     6661 cttcggtatc taactcttga agggcttttt cttctggtgt ttgttcttgt atcgctgcga
     6721 tatctagctg aagttggatt aaccgtttat caatctcggg aacgactaga tcagaataac
     6781 gagcaatgcg ttctgggata cccgagcgta atgcctctag catggggtgc acaggcttta
     6841 actcatcttg atacacttgc cgcaataaac cagcggtaat acgctcattg cctaaagcta
     6901 gcgcacggag ctgagcgagt acaaaaagtt ttactacaat gtccatcact ccttggctta
     6961 gctcatacca cacatcacgg acctcatccg ataacagcgc atctttgcgt tgtaaaagct
     7021 gtaattgcca aagattatcc gtaaaagcga tccactcttg attgggcttt ccacgttgcg
     7081 tttgttgtat aggatcccag aatatagctc caaaccctgc ccctctacgt gcagaccgca
     7141 aatcagcctc aaaaatctct cgtgctttag gggtaccaat caacatcact ggtacgccaa
     7201 taatattcac catcgtcaca aaaaagttca gcatctcttg agatccaccc gaacgagagc
     7261 ggcttaaatg ctgaatttca tcaataacca acaaccctaa agcatgtgca ttggctattt
     7321 gcgacatcaa agccaacatg gtttctatac catgacgttt taagccataa cgacgctcat
     7381 agttcgagcc caaggctcga tccaacgctc tgaaaaaatt caagcagatt tcttttagcg
     7441 aaccattatg cgagcagtct attttcaaat acaccacctg ctctacattg agttcacgat
     7501 ggtaaatcac ctgaggatac gtggctagaa tacgatgaag agaggtcgtc ttcccactac
     7561 cagaacaacc aattaacaat aagctttgtg ccgtagatcg tgcctcctca aagcgaaatg
     7621 tctccaactc tcccgtttga acacgctcat aaccattttg taaatgcttt tgtaaatctc
     7681 ctgttttagg atttctgcct acgtagccac ctcgaatcat gaccgaaata cgctcactta
     7741 gtagcaaatg cgtacctaat ggctgaaaat agtcatctgg aatacgacaa atggtatgag
     7801 ctctgataac acgggacttt tgcaagtcag aggaagtaag ctgtaaagag gatttcagtg
     7861 atgcagcact attcactgac tcttgtaatg gtggtaaggc ctcgataaaa ggattatcac
     7921 gataagcctc tacccccgta tcacgataaa ctgcttgaat ccgggtagca ctcatgactc
     7981 atccttttct ggtggatcct gaaataattc aggcacgtat gtgggtaggc tgtaatcttc
     8041 ttgatcatcc gcttccactg cgttgaaagg aataacttta gcctcatcac ctgaagagct
     8101 tggcttcaaa tgctccgcac gttttttacg ctccgaggtc acggcttctt ttttattagt
     8161 tttaatctgc ttaatgcgtg ttgatttggg ctcagtagta ctgggcgtta acttattcgc
     8221 tttctgaatg gtttgctgaa taaacgcctc aagctccctg cgtttagtta actcatcctg
     8281 cttcgcattg gctttattgt gtttttcttg tgcttgtata tcccaaacct cccaaaatga
     8341 gagaccttta aactgccgac tacgttccgt cagattacag cgccaaaata cacggctgcc
     8401 aacttgcgga aacaaataaa tcgtatcaac cagcactggg tcataagccg cttctaaatg
     8461 ttgaggtcta gctatatcag tgctccgctg caaccaaccc tcacgcagaa tctctgaccc
     8521 cgagtaatac aaaccccaca aattaacgcc aaatgaagaa atagagacct ttcggcgagg
     8581 cagtaacgct actcgcaact gctcttgctc cacagccctt aaactacctg tacgatgctg
     8641 cataccccat tgccatagct ggacaggaat agacggtaaa tctgtaggaa aatcagcatc
     8701 tcgatcgtat ttatccatca ccagatggtt atttctgaat aagatcgtac gcaaaataat
     8761 ttgtgtgaac tcaaataccg acagagatgc atctaaccta tagtctgttt caccatggct
     8821 tttgatccga ctgccctcta caatgccagg tgcaaaggac ttaaactcgg cttgtagtgt
     8881 tctaaaagtg ctttccacta tgcctttagc atcgccacgt ctaggtggag cactttccac
     8941 tcgcacatta aaactagaaa ctaaggcttc gacctgatga ctcattaatt cgccacggtc
     9001 cgctagcaac acatctggca aacctacaca cggccagtct gagctactaa tctcaatatc
     9061 atgctgggca caaatggccg ttttgtcaga gcaagcattt acaaaagcct gcatcgccac
     9121 cacataagac ggattttcaa agccgatata aaagcccgtg atcatccgac taaacacatc
     9181 aatcacaatg taaagcgttg gtcttcctat gattttttgg cgatcatgat gatccactaa
     9241 ataaatatca gcaatcgtgg catcaatctc ataacgactc ccagggccta acgcctgaga
     9301 agtggctgta ctacttaagg gtcgtacgtc ttttttatat acccctgctt taactctaga
     9361 ctttaagcgc tgagctttag ggtattctcg atcataaaaa taacgaaact gacgtagtgt
     9421 tgggtaatcc tcttggggaa tgcgaggaaa atactgagca aacaagtcca caaatcgtct
     9481 ataggcaacg gtggtctttg tacctttttg atttaacagg tgcttttcta tggtcaacct
     9541 aaaaaggcgt tcaatctcgg gcgttacctt ggttccttca cccttaccat attctctggc
     9601 tcggccaatc tttgctgttc ctgtcgctga acgtctttcc cctggtgcac cgctgttttt
     9661 gtagtcagga attaatgcat taggcgtttg accacgctgc cagtaacggc gtaacaactt
     9721 ataaactgta gccttagtaa ccttatgttc ttggaccaca tgctcaacga gttcgcttct
     9781 gactttaggg tcgaaacgat ccttactatt aataatagga agaatttttc gataatcctc
     9841 ctcgcgcttc tggaagctga cagaatctac agacggctct tctaaatcaa gatgtacata
     9901 agggtcatca gttctaacta agcggccttc atctaaatac tgcattagca acaactcagc
     9961 cctagcttgt ggaactcctt tatcagcgct tatttgcatc cagacaactt ggccatcctc
    10021 tatagccaaa atgcgatacg gatcattatc aaatagcaca acctcattaa tttgccacat
    10081 agcgcaactc ctccatattc actacttggc taatacagag atctgcacac ttatttgccc
    10141 tgaaagactt ataaatattg aacttaataa aaccatttgc ggttaaggct cgtatctcac
    10201 tcaatgtttt gcctaactcc aaatcataag caatatcaac ctgcttacag acattgataa
    10261 tgttttcatc tcctttttct tgcaggatat gggccaatgg ggatagttgt gctaaaagct
    10321 ccgcagaaac ttcttctgtt ttcactgaat aaagccattc aatattttct tttactacgg
    10381 gatttatttc tttatcagta aaaatgaacc aaggaatttg cttttgctgc caatagcgac
    10441 gctctagttc tagtttttct aaggtacgct cgtcttgtaa ggctgctgca ggtttgactt
    10501 gaatagcaaa ctgctcaaaa ggaccatctt tgcagtccac taaaaaatca gtagacataa
    10561 cctgatctac accacgaata acaggatgct taataccact atctattgca atctgcctgg
    10621 tatcactagg taataagggg aactgctcgc gtatatctag cacgctgctc tcccactcaa
    10681 gactgagaaa aacagcaagc tctaagtcag atagcaaatg atggactcgt cccgtcttat
    10741 gagaataaat acggtgggaa cgacctgaag aaggaacttc ttgtactgtt agccatggaa
    10801 tatagtcttt accatgccct tggccacgcc cctctttaat acgacgggca atttgcactt
    10861 cagaaaatga agagtttgct ttagccataa caaaagtcca gtatgctttt tcacagcata
    10921 actggactga tttcagttta caactattct gtctagttta agactttatt gtcatagttt
    10981 agatctattt tgttcagttt aagactttat tgtccgccca caagcccttt tttatctatg
    11041 atagtgatta ctcaaccgta accgattttg ccaggttacg cggctggtca acgtcggtgc
    11101 ctttgatcag cgcgacatgg taagccagca gctgcagcgg aacggtgtag aagatcggtg
    11161 caatcacctc ttccacatgc ggcatctcga tgatgtgcat gttatcgcta cttacaaaac
    11221 ccgcatcctg atcggcgaag acatacaatt ggccgccacg cgcgcgaact tcttcaatgt
    11281 tggatttcag tttttccagc aattcgttgt tcggtgcaac gacgataacc ggcatatcgg
    11341 catcaataag cgccagcgga ccgtgtttca gttcaccagc agcgtaggct tcagcgtgaa
    11401 tgtaggagat ctctttcagc ttcaatgcgc cttccagtgc gattgggtac tgatcgccac
    11461 ggcccaggaa cagtgcgtga tgtttgtcag agaaatcttc tgccagagct tcaatgcgtt
    11521 tgtcctgaga tagcatctgc tcaatacggc tcggcagcgc ctgcagacca tgcacaatgt
    11581 catgttcaat ggaggcatcc agacctttca ggcgagacag cttcgccacc agcatcaaca
    11641 gcacagttaa ctgagtggtg aatgctttag tggacgccac gccgatttcc gtacccgcgt
    11701 tggtcatcag cgccagatcg gattcgcgca ccagagaaga acccggaacg ttacagattg
    11761 ccagcgaacc aaggtaaccc agctctttcg acagacgcag gcccgccagg gtatccgcgg
    11821 tttcgccaga ctgtgacaag gtgatcatca ggctgttacg acgcacggca gatttacgat
    11881 agcggaattc agaggcgatt tcgacgtcgc acggaatacc tgccagcgat tcaaaccagt
    11941 agcgggaaac cataccggag ttataagaag taccacaggc gaggatctga atatgctcaa
    12001 ccttcgacag cagttcgtcg gcgttcggtc ccagctcgct taaatcaacc tgaccgtggc
    12061 tgatgcgccc ggtaagggtg ttttttatcg cgttcggctg ttcgtagatc tctttctgca
    12121 tgtagtgacg gtaaatacct ttatcgcccg cgtcatattg cagattggat tcgatatcct
    12181 gacgttttac ttccgcgcca gttttatcga agatgtttac cgaacggcga gtgatttccg
    12241 caatatcgcc ctcttcaagg aagataaagc gacgggtcac cggcaacagc gccagctggt
    12301 cagaagcgat aaagttttcg cccataccca ggccaatcac cagcggacta ccagaacgtg
    12361 ccgccagcag ggtatccggg tgacgggagt ccatgatcac tgtaccgtac gcaccgcgca
    12421 gctgcgggat agcacgcaga acagcctcac gcagagtccc gccttgtttc agctcccagt
    12481 tcaccagatg ggcaatcact tcggtgtcgg tttcagaaac gaaggtatag ccacgcgctt
    12541 ttaactcttc acgcagcggt tcatggtttt cgatgatgcc gttatgcacc accacaatgt
    12601 gttcagaaac atgcggatgc gcgttagctt ctgaaggttc accgtgggtc gcccagcgag
    12661 tgtgagcaat accggtgccg ccatccagag gatgttcttc cgctgcctga gccagcatct
    12721 ggactttacc gaggcgacgc aggcgggtca tatgaccttc cgcatcaaca acggccagac
    12781 cggcagagtc atatccgcgg tattccagac gacgtaaacc ttcaagaagg atttctgcta
    12841 catcacgttg cgcgatcgcg ccaacaattc cacacatagt ttttgattcc gatttatatc
    12901 gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc gagccttgta gagagtgggg
    12961 ttatttttat agttactgct tgtgggaggg agattatgtt atctcctcat cccagatgac
    13021 cggattaccc ggccagaatt actttttctt taccggacga cgccagcctt ctttctgagt
    13081 ctgcggcaca cggctgatag ctaatgcatt ttcgccgaca ttacgcgtca cagttgtacc
    13141 cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc accagctgag tgtcggaacc
    13201 gacaaacaca tcgtcgccga taatggtctt aaatttattc gcaccatcgt agttgcaggt
    13261 aatggttccc gcgccgatgt taacgttatc gccaatttcc gcatcgccca ggtaagtcag
    13321 atgcccgact ttcgagcctt tacccagacg cgcttttttc atctcaacga agttaccaac
    13381 gtgtgcacct tccagcaact cagcaccagg acgcaaacga gcaaacgggc caatagtaca
    13441 ggccgctgcc agattcgcgt cttccacaac ggtatacgga ctgatttcgc aatcatcgcc
    13501 aatcacgctg tttttaatca cgcaaccggt gccgattttc acgcgatgac cgagagtcac
    13561 gttgccctcg atgataacgt tagtatcaat ttcaacatcg cgcccgtgag taagcgtacc
    13621 gcgcagatca aaacgcgccg gatcgcgcag cataacgcct gctaacagca gtttttcagc
    13681 ctgttcggac tggtaaacgc gctccagacg ggagagttgc aggcggttat tcacaccttc
    13741 tacttcgctt aaacgttgcg gatgaacggc gacgatttca cgcccttcct gatacgccag
    13801 cgcaataatg tcggtgatgt agtattcgcc ctgagcatta ttgttggtca gcttcgccag
    13861 ccagcgtttc atatctgcgc cgttagcgat cagaatgccg gtgttgatct cctgaatctg
    13921 acgctgctcg tcggtggcat ctttgtgctc aacaatgccg gtaactttgc cattttcacg
    13981 ggtgatacgt ccataaccgg tcggatcatc cagtttcacc gtcagcagac caatgccacc
    14041 ctgcggttta gcatcacgca ggcgctggag tgtttcgaca gagatcagcg gcacgtcgcc
    14101 gtagagcatt aaaatgtctt catcatcggc aaagaaaggt gcggcctgct gcattgcatg
    14161 acccgtaccc agctgctctg cctgaagcac ccagttaagg ttgtcgtctt tcagcgcctg
    14221 tttaagcaga tcgccgccgt gaccgtacac caagtgaacg tgcgctgcgc ctaattcatt
    14281 cgcagcatca atgacatgct gaaccatcgc tttcccggca agggtatgca gcactttcgg
    14341 aagatcggaa tacatgcgcg tgcctttgcc tgcggcaagg atcactacgc tcatagcatt
    14401 attcaacata cgcgtcctga ctgtaatttg agaacgaatt taaaccgctt caccttgaaa
    14461 aaactacatt tttttcatcg tgaaatggac agaggataaa ttgttcaata acggattatc
    14521 cccgctgacg aaagtgccat tttcgaccat cgtcacgcct tttgtctgca gaaaattaag
    14581 gcagtacctt catttaagcg tcaggtggat gtttttgctc ttatttcgat caatgaataa
    14641 acgggaaaac aggggtgttt tatatcagca ggatctatgt gaacgctctt caggacggat
    14701 cacgcgtaaa aaaaagccag cctgtttcca gactggcttt tgtgcttttc aagccggtgt
    14761 tacatcgctt ttttggtcaa ctcgataacg cgcagctgcg cgatcgcttt ggccagttcc
    14821 gcagacgcct gagcgtaatc tacgtcgccg tgagagctgc taatgtgctc ttcagcctta
    14881 cgtttcgctt ccatggctcg cgcttcgtcg agatcctggc cgcgaattgc ggtgtcggcc
    14941 agaacggtca cgttgccagg ctgcacttca agaatgccgc cagacagata gataaactct
    15001 tcgtgaccgt gctgtttcac gatgcgaatc ataccaggct taatggcggt gagcagcggt
    15061 gcgtggccag ggtagatccc cagttcacct tcgctacccg ttacctggat tttctcgacc
    15121 agaccagaga acatttgttg ctctgcgctg acgacgtcca ggtggtaagt cattgccata
    15181 tcaccctccg attaaggcgt taaagttttt tggctttttc cacagcttct tcgatggaac
    15241 cgaccatgta gaacgcctgc tccggcaggt gatcgtattc gccttccatg atgcctttaa
    15301 agccacggat ggtgtctttc agggagacgt atttacccgg agaaccggtg aatacttctg
    15361 ccacgaagaa cggctgggac aggaagcgct ggatcttacg agcacgcgct accaccagtt
    15421 tgtcttcttc agacagttca tccataccca agatggcgat gatgtctttc agttcctgat
    15481 aacgttgcag gatggactga acgccacgcg cggtgtcgta gtgttcctga ccaaccacca
    15541 gcgggtccag ctgacggctg gtggagtcca gcgggtcaac ggccgggtag atacccagag
    15601 acgcgatctg acggctcagt accacggttg cgtcaaggtg cgcaaaggtg gttgccggag
    15661 acgggtcagt caagtcatcc gcaggtacgt ataccgcctg tacggaggtg atagaaccag
    15721 ttttggtgga ggtgatacgt tcctgcagaa cgcccatctc ttccgccagg gtcggctgat
    15781 aacctaccgc tgaaggcata cggcccagca gtgcggatac ttccgtaccg gccagggtgt
    15841 aacgatagat gttgtcaacg aacagcagaa cgtcacgacc ttcgtcacgg aatttctcag
    15901 ccatggtcag accggtcaga gcaacgcgca gacggtttcc cggcggctcg ttcatctggc
    15961 catacaccag ggatactttg tcgataacgt tggagtcggt catttcgtgg tagaagtcgt
    16021 taccctcacg agtacgttca cctacgcccg caaacacaga gtaaccggag tgctcgatcg
    16081 cgatgttacg aatgagctcc atcatgttta cggttttacc tacacccgca ccaccgaaca
    16141 gaccaacttt accgccctta gcgaacggac acatcaggtc gataactttg ataccggttt
    16201 ccagcagttc ctgagagttt gacagctctt cgtaggaagg tgctgcgcgg tgaatcgccc
    16261 aacgctcttc ttcaccgatc tcgcctttca tgtcgaccgg ttcacccagt acgttcatga
    16321 tacggcccag agtcgcttta cctaccggga cttcgatcgg gtgttcgagg tcttttacat
    16381 ccagaccgcg acgcagaccg tcggaggaac ccattgcgat ggtacgcacg ataccgccgc
    16441 cgagctgctg ctgaacttcc agcaccagac gctcattacc attttgcacc tcaagagcat
    16501 cgtacacgcg cggtacggca tcctgaggga attcgacgtc aactacggcg ccgattacct
    16561 ggacaatctt tccagtagcc atcttaaatc ctctacgaaa taacctgttt aaaccgcggc
    16621 ggcccccgag acgatctcgg tgagttcctg agtaatgctg gcctgacgag ctttgttgta
    16681 taccaactgc agctctttaa tcaggctgcc gccattgtcg gtcgcggctt tcatcgccac
    16741 catacgggcg gcctgctcgc tggccaggtt ttcaaccacg ccctgataaa cctgagattc
    16801 gacataacga cgcagcaggg tatccagcaa cgccttcgga tcgggttcgt acaggtaatc
    16861 ccaggattta tgtttcagat catcatcatc tgatgccggt aacggcagca gctggctgat
    16921 ggtcggaacc tgagacatgg tgttaataaa tttgttgctg acaatgtaaa gcttgtccag
    16981 acggccttcg tcgtaagcct gcaacatcac ttttaccgga ccgatcagtt cggacaggga
    17041 agggttatcc cccatgccgg tgacctgggc aacaacattg ccgcccacgg agttgaagaa
    17101 cgacacgcct ttcgagccga tcattgcgag gtcgcattga acgcttttgt cggtccaggt
    17161 cttcatttcc gccagtagtt ttttgaacag gttaatgttc aaaccaccgc acaaaccacg
    17221 gtcggtcgac accaccaggt agcccacgcg tttaacgtcg cggtcttcca ggtaagggtg
    17281 cttatattcc agattaccgt gtgcaaggtg accaatcact ttgcgcatgg tttctgcata
    17341 aggacggctg gccgccatgc gatcctgcga tttacgcatt ttggaagcgg cgaccatctc
    17401 catcgcttta gtgatctttt gcgtgttctg gacgcttgcg atcttactac gtatctcttt
    17461 tgcgccggcc atgagcttct cctcaatgcc ttgcggcctg ccctaaggca agccgccaga
    17521 cgttaccagg attgggttgc tttgaaggaa tcgaggatgc ctttcagctt gccttcgatt
    17581 tcgtcgttgt agccaccggt ctggttgatc tcttgcatca acggagcgtg atcacggtcg
    17641 acgtaagcca gcagagcggc ttcgaagctg ccaattttcg acagttcaac atccgccagg
    17701 taaccacgtt ctgctgcgaa cagaaccaga gactgctgcg caacggacat cggcgcatac
    17761 tgtttctgtt tcagcagttc ggtcactttc tgaccgtggt caagctgctt acgtgttgca
    17821 tcgtcaaggt cggatgcaaa ctgagagaac gctgccagtt cacgatactg tgccagagcg
    17881 gtacggatac caccggacag ttttttcatg atcttggtct gtgctgcacc accaacacgg
    17941 gatacggaaa tacccgggtt aaccgcagga cgaataccgg cgttgaacag gttggtttcc
    18001 aggaagatct gaccatcggt aatggagatt acgttggtcg gaacgaacgc agaaacgtca
    18061 cccgcctgag tttcgataat cggcagtgcg gtcagagaac cggttttccc tttcacttca
    18121 cctttggtga aggcttcaac gtattcggcg ttaacacgtg cagcacgctc cagcagacga
    18181 gagtggaggt agaaaacgtc gcccgggaat gcttcacgtc ctggcggacg acggagcagc
    18241 agggagatct gacggtaagc aacagcctgt ttagacaggt catcgtaaat gatcagcgca
    18301 tcttcaccgc ggtcacggaa gtattcgccc attgcgcaac cggcatacgg tgccaggtat
    18361 tgcagtgcag cggattcaga cgcggttgct accacaacga tggtgttagc cagtgcgccg
    18421 tgctcttcca gtttacgtac cacgttagaa atggtggacg ctttctggcc gatagcgaca
    18481 tagatacatt tgataccgga atcgcgctgg ttgatgatgg catcgatagc cagtgcggtt
    18541 ttacctgtct gacggtcacc gatgatcaat tcacgctgac cacgaccgat tgggatcatg
    18601 gagtcaacgg ctttataacc ggtctgtacc ggctgatcta cggactgacg ttcgataacg
    18661 cccggagcga ttgcttctac agcagagaag ccgtcgtgat ccagcggacc tttaccgtcg
    18721 attggtgcac ccagagtgtt aaccacacgg cccagcaggc cacggccaac cggaacttcc
    18781 aggatacggc cagtacactt aactttcatg ccttcggcaa ggtcagcgta cggacccata
    18841 acaaccgcac ctacagagtc gcgctcaagg ttcagtgcga tagcgtaacg gttacccggc
    18901 agggagatca tttcaccctg catacaatcg gccaggccgt gaatgcggat aacaccgtca
    18961 cttacagaaa caatagtacc ttcgttgtga gcttcactca caacattgaa ctgagcaatg
    19021 cgctgcttga tcagttcgct gatttcggtg gaattcagtt gcatgctcca gtccccttaa
    19081 gactgcaaga cgtctgcaag gcgctcaaga cgaccgcgta cgctgccatc aatgaccata
    19141 tcacccgctc ggatgataac gcctgccatt acagacttat cgattttgca attcagctta
    19201 actttgcgtg acagacgttt ttccatcgca gcagaaattt tcgcgagctg ttgttcactc
    19261 agtgcggcag cggaaatgac gtctacctca gcggtagcct cactcacggc acgaaggtga
    19321 ataaactgct ccagaacatc cgggagcgcg ttaagacgac cattttcagc cattacccga
    19381 atcaggttct gaccgttttc gtccagttgc tcaccacaaa ctgcgataaa cgactcggcg
    19441 agcgtttctg gcgcaagcgc gccagagaga agctctgcca tttgttcgtt tttggttacc
    19501 tcggcggcaa acgccagcat gtcctgccag cgttctacac tttggtgttc gacggcaaag
    19561 tcaaaagctg ctttggcgta ggggcgagct accgtaataa attcagacat cagcccctcc
    19621 ctccttacag ttcagcgaca agtttatcca cgatgtcgct gttagcagct tcatccacgg
    19681 aacgttcgat gatcttctcg gcgccagcaa cagccaggat agcaacttgc ttacgcagct
    19741 cttcacgggc acgtttacgc tcggcttcaa tttctgcctg cgcctgggcc acgattttag
    19801 tacgttcctg ttctgcctca gctttcgctt cgtccagaat ctgcgagcgg cgtttgttcg
    19861 cctgctcgat gattacctgg gcttccgctt tcgctttttt cagctggtcg gtcgcgctgg
    19921 cctttgcaag gtcaaggtcc ttatgtgctc gttctgcgga agcaaggccg tcagcaattt
    19981 ctttttgacg tttttcgatg gctgccatta atggcggcca tacgtacttc atgcagaaca
    20041 gaacgaacag gacaaacgcg atggcctggc cgaggattgt tgcgttaaga ttcacagcac
    20101 aatgcctcta tttagttaac gttctgatat tgctctttaa ataaaagcaa cgcttactac
    20161 gcgacagcga acatcacgta cagacccaga cctacagcga tcatcgggat agcatccacc
    20221 agacccataa cgataaagaa ctgagtacgc agcagaggaa tcagatcagg ttgacgcgct
    20281 gcgccttcca ggaatttacc cccgaggatg ccgataccga tcgcagcacc gattgccgcc
    20341 agacccatca tcacagcggc agccatgtac agcagatcca tattcaggtt ttccatgaca
    20401 gtctccagtt tgtttcagtt aaaacgtagt agtgttggta aattaatgtt cttcagacgc
    20461 catcgacaga tagacgatcg tcagaaccat gaagatgaag gcttgcagcg taatgatcag
    20521 gatgtggaaa atggcccacg gcacattcag gatccactgt gaccaccacg gcaacagacc
    20581 agcaatcaga atgaaaatca gctcaccggc atacatgtta ccgaacagtc gcaaaccgag
    20641 tgaaactggt ttggacagca ggcttacccc ttcaaggatt aagttgacag gaatgaacgc
    20701 ccagtgattg aacggctgca gcgtcaactc tttcgtgaag ccgccgatgc ctttcatttt
    20761 gatgctgtag aacagaatca ggataaatac gcccagtgcc atagacagcg ttacgttcac
    20821 gtccgcagac ggaaccacac gcagtgcagg cagacccagt acatgttcag caatgtacgg
    20881 cagcaggtcg ataggcagta aatccatcag gttcatcagg aatacccaga cgaagatcgt
    20941 cagggccagc ggagcaatca gcttgctttt gccatggtac atgtctttca cgctaccatt
    21001 aacaaagccg atcaccagct caatcgcggt ctgaaactta cctggcacac cgctggtcgc
    21061 ctttttggct acgctacgga ataaaaccag gaacaacaga cccagcacca ccgagaagaa
    21121 catggagtca atattgattg tccagaaggt ggctgggggg ttttgtggat ccaccagcga
    21181 gaatgtacgc aggtccagct gaaggttatt caggtggtgt cctatgtaat cctgcggcgt
    21241 catattttct gaagccatga tgccttttac cctttgttgt taattacagc cggtgccagt
    21301 atctgaacca ccagcaccaa aacccacgta acgatcagcg gcaagaatac cgcctttaaa
    21361 accgccaacg ccaccaccag taacaccaac atcgccagaa ctttgaaagc ttcgccaaat
    21421 gcgaatgtcc aggccacccg gcctttcgct ggtgtatacg cctggtgacg ccaggcaaat
    21481 atcataaaca aaacgttagg cagaaagact gccaggcccc cgcttattgc agagacgccc
    21541 cagaaggggt ctttgaggct gaacagcaat ccacttgcta tcaccaccag taactgaacg
    21601 agcagaagct tccgagcaac gtttcgactc acgagcgaca cagacatcac gtttttcact
    21661 cctgctccct tcgaggtatg ccgcgtgtcg tataaaactt tctttaaggc ttagagtcaa
    21721 gcatcaaaaa gcggtcaaat tatacggtgc gcccccgtga tttcaaacaa taagtagcca
    21781 aaaggtgaat aaatgtttaa atatttttcc agtgcataca attgcgactt ttccgctaac
    21841 cctgttcgat catgaaaaac tgtaaataac gcgtaaacac tagtgataaa gcgtgcttca
    21901 gatcacatat tgcgcatgtt cgcgcacagt atatttattt acttggcaaa
//
LOCUS       DAZOZD010000050        21642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOZD010000050 DAZOZD010000000
VERSION     DAZOZD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.050"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUPB_001532"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUPB_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0758383.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUPB_001533"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUPB_001533"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0758384.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUPB_001534"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUPB_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0758385.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="ACHUPB_001535"
     CDS             complement(3716..4510)
                     /locus_tag="ACHUPB_001535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0758386.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="ACHUPB_001536"
     CDS             4648..4989
                     /locus_tag="ACHUPB_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0758387.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUPB_001537"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUPB_001537"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0758388.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUPB_001538"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUPB_001538"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0758389.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="ACHUPB_001539"
     CDS             8905..10197
                     /locus_tag="ACHUPB_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0758390.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10228..10325
                     /locus_tag="ACHUPB_001540"
                     /pseudo
     CDS             10228..10325
                     /locus_tag="ACHUPB_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUPB_001541"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUPB_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0758391.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="ACHUPB_001542"
     CDS             complement(10730..11188)
                     /locus_tag="ACHUPB_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0758392.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="ACHUPB_001543"
     CDS             complement(11185..12102)
                     /locus_tag="ACHUPB_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0758393.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUPB_001544"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUPB_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0758394.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUPB_001545"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUPB_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0758395.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUPB_001546"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUPB_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0758396.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUPB_001547"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUPB_001547"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0758397.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUPB_001548"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUPB_001548"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR0758398.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUPB_001549"
     CDS             16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUPB_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0758399.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUPB_001550"
     CDS             16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUPB_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR0758400.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUPB_001551"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUPB_001551"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0758401.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUPB_001552"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUPB_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5144 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccgcggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgaccaggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccaatgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccagtttta tcgaacacga tggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttta actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAZOZD010000051        21637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOZD010000051 DAZOZD010000000
VERSION     DAZOZD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.051"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUPB_001553"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUPB_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0758402.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUPB_001554"
     CDS             567..650
                     /locus_tag="ACHUPB_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758403.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUPB_001555"
     CDS             750..1571
                     /locus_tag="ACHUPB_001555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0758404.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUPB_001556"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUPB_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0758405.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUPB_001557"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUPB_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0758406.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUPB_001558"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUPB_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0758407.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUPB_001559"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUPB_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0758408.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUPB_001560"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUPB_001560"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0758409.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUPB_001561"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUPB_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0758410.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUPB_001562"
     CDS             8347..9729
                     /locus_tag="ACHUPB_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0758411.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUPB_001563"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUPB_001563"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0758412.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUPB_001564"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUPB_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0758413.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUPB_001565"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUPB_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0758414.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUPB_001566"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUPB_001566"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0758415.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUPB_001567"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUPB_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0758416.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUPB_001568"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUPB_001568"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0758417.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHUPB_001569"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHUPB_001569"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0758418.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="ACHUPB_001570"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="ACHUPB_001570"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0758419.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="ACHUPB_001571"
     CDS             18642..20150
                     /locus_tag="ACHUPB_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0758420.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..>21637)
                     /gene="uidC"
                     /locus_tag="ACHUPB_001572"
     CDS             complement(20331..>21637)
                     /gene="uidC"
                     /locus_tag="ACHUPB_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0758421.1"
                     /translation="KIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRADK
                     PSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGDKS
                     FGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALGYW
                     ALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSR
                     YSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALV
                     SGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYR
                     LTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGRE
                     KRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
BASE COUNT         5410 a         5587 c         5224 g         5416 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttc
//
LOCUS       DAZOZD010000052        21445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOZD010000052 DAZOZD010000000
VERSION     DAZOZD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.052"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(321..866)
                     /gene="hemG"
                     /locus_tag="ACHUPB_001573"
     CDS             complement(321..866)
                     /gene="hemG"
                     /locus_tag="ACHUPB_001573"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0758422.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(878..2329)
                     /gene="trkH"
                     /locus_tag="ACHUPB_001574"
     CDS             complement(878..2329)
                     /gene="trkH"
                     /locus_tag="ACHUPB_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0758423.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2368..2982)
                     /locus_tag="ACHUPB_001575"
     CDS             complement(2368..2982)
                     /locus_tag="ACHUPB_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0758424.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2982..4313)
                     /gene="pepQ"
                     /locus_tag="ACHUPB_001576"
     CDS             complement(2982..4313)
                     /gene="pepQ"
                     /locus_tag="ACHUPB_001576"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0758425.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4503..6692
                     /gene="fadB"
                     /locus_tag="ACHUPB_001577"
     CDS             4503..6692
                     /gene="fadB"
                     /locus_tag="ACHUPB_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0758426.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6702..7865
                     /gene="fadA"
                     /locus_tag="ACHUPB_001578"
     CDS             6702..7865
                     /gene="fadA"
                     /locus_tag="ACHUPB_001578"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0758427.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8055..8756)
                     /gene="fre"
                     /locus_tag="ACHUPB_001579"
     CDS             complement(8055..8756)
                     /gene="fre"
                     /locus_tag="ACHUPB_001579"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0758428.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8803..10296)
                     /gene="ubiD"
                     /locus_tag="ACHUPB_001580"
     CDS             complement(8803..10296)
                     /gene="ubiD"
                     /locus_tag="ACHUPB_001580"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0758429.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10463..10951
                     /gene="rfaH"
                     /locus_tag="ACHUPB_001581"
     CDS             10463..10951
                     /gene="rfaH"
                     /locus_tag="ACHUPB_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0758430.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10948..11730)
                     /gene="tatD"
                     /locus_tag="ACHUPB_001582"
     CDS             complement(10948..11730)
                     /gene="tatD"
                     /locus_tag="ACHUPB_001582"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0758431.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11772..12548)
                     /gene="tatC"
                     /locus_tag="ACHUPB_001583"
     CDS             complement(11772..12548)
                     /gene="tatC"
                     /locus_tag="ACHUPB_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0758432.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12551..13066)
                     /gene="tatB"
                     /locus_tag="ACHUPB_001584"
     CDS             complement(12551..13066)
                     /gene="tatB"
                     /locus_tag="ACHUPB_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0758433.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13070..13339)
                     /gene="tatA"
                     /locus_tag="ACHUPB_001585"
     CDS             complement(13070..13339)
                     /gene="tatA"
                     /locus_tag="ACHUPB_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0758434.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13418..15058)
                     /gene="ubiB"
                     /locus_tag="ACHUPB_001586"
     CDS             complement(13418..15058)
                     /gene="ubiB"
                     /locus_tag="ACHUPB_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0758435.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15055..15660)
                     /gene="ubiJ"
                     /locus_tag="ACHUPB_001587"
     CDS             complement(15055..15660)
                     /gene="ubiJ"
                     /locus_tag="ACHUPB_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0758436.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15674..16429)
                     /gene="ubiE"
                     /locus_tag="ACHUPB_001588"
     CDS             complement(15674..16429)
                     /gene="ubiE"
                     /locus_tag="ACHUPB_001588"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0758437.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16524..17951)
                     /gene="rmuC"
                     /locus_tag="ACHUPB_001589"
     CDS             complement(16524..17951)
                     /gene="rmuC"
                     /locus_tag="ACHUPB_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0758438.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18092..18853)
                     /gene="udp"
                     /locus_tag="ACHUPB_001590"
     CDS             complement(18092..18853)
                     /gene="udp"
                     /locus_tag="ACHUPB_001590"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0758439.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19115..19927
                     /locus_tag="ACHUPB_001591"
     CDS             19115..19927
                     /locus_tag="ACHUPB_001591"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0758440.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20321..21004
                     /gene="tssD"
                     /locus_tag="ACHUPB_001592"
                     /pseudo
     CDS             20321..21004
                     /gene="tssD"
                     /locus_tag="ACHUPB_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            20984..>21445
                     /locus_tag="ACHUPB_001593"
     CDS             20984..>21445
                     /locus_tag="ACHUPB_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758441.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5261 a         5737 c         5376 g         5071 t
ORIGIN      
        1 acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag tgcccacaca
       61 gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc ggcgcggggt
      121 gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc tctgtcggaa
      181 ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg
      241 cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt ttctgaccgc
      301 gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct
      361 cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt
      421 tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca gcgataacgt
      481 gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg cgagttcatc
      541 agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc
      601 aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca
      661 aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg
      721 tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc
      781 cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt
      841 gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg
      901 taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga
      961 tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc
     1021 ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc acgcccgtgg
     1081 cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa
     1141 cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt
     1201 tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga
     1261 tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa
     1321 gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg
     1381 tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga
     1441 cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa
     1501 acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa
     1561 agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata gtgttaatag
     1621 tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag
     1681 tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg
     1741 cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc
     1801 gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc
     1861 cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa
     1921 accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca
     1981 gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg
     2041 tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc
     2101 agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc tctttacggt
     2161 tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc
     2221 ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa
     2281 ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt
     2341 ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc
     2401 acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac
     2461 ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag
     2521 caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt cggttaatgg
     2581 cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt
     2641 cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagaggtaat
     2701 ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcctgccgg
     2761 ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc
     2821 cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc
     2881 cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt tgatctcttc
     2941 aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc
     3001 cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat acgaatgccg
     3061 ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca
     3121 cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt taacaccata
     3181 cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt
     3241 gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatga
     3301 ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc
     3361 atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg gaactggatg
     3421 tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag ttgttcatca
     3481 tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc
     3541 aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct
     3601 tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc
     3661 acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg
     3721 ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg
     3781 accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg
     3841 tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt
     3901 tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc
     3961 aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag
     4021 gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc
     4081 ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac
     4141 gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac
     4201 agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc
     4261 cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac
     4321 ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga
     4381 tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat atttttaaca
     4441 caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg
     4501 acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac
     4561 tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg
     4621 gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga
     4681 acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc
     4741 ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag
     4801 atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat
     4861 gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa
     4921 ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg
     4981 ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga
     5041 aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg
     5101 ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag ccgaagctgg
     5161 aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct aaaggaatgg
     5221 tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa accattgaag
     5281 ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt tttgtcccgc
     5341 tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat caatatgtaa
     5401 aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct gctgtgctgg
     5461 gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc gtgccggttg
     5521 tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc
     5581 tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc gtgatctcca
     5641 cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg gtagaagcgg
     5701 ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc
     5761 aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc
     5821 tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac cgaatgccgt
     5881 tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct
     5941 gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta
     6001 accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt
     6061 tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc ccggcatatc
     6121 tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct
     6181 tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc
     6241 gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc
     6301 cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc
     6361 gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg
     6421 tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct
     6481 acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta
     6541 gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc
     6601 cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag
     6661 cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg
     6721 tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg
     6781 cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag
     6841 cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta
     6901 atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg gcggttaccg
     6961 tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga
     7021 ctggcgatgc gcaggcaggt ctggttggcg gcgtggagca tatgggccat gtgccgatga
     7081 gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga
     7141 tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg
     7201 cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa
     7261 atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg
     7321 acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg
     7381 atccagtaag cggtacggta acggcgggta catcttctgc actttctgat ggtgccgcgg
     7441 ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg cgcgcccgtg
     7501 tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc ccggttcctg
     7561 cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga
     7621 tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc
     7681 agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggtt
     7741 gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa gacgttcagt
     7801 ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg
     7861 tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat
     7921 gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg
     7981 acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg
     8041 cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc ttcccgcgca
     8101 ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg tccggcaata
     8161 tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa cacggtgcca
     8221 gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg cagaccagga
     8281 tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc ttcacgccca
     8341 ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa taaaatcgag
     8401 cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc ttcatcatcg
     8461 cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt gaggatgcgg
     8521 tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag ctcgataaac
     8581 cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc atccatcact
     8641 accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat tcggacacgg
     8701 tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt tgtcatgcgc
     8761 tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc ttttaccgtt
     8821 gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat caggatcttt
     8881 tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg tggcatccag
     8941 ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat caataggcgt
     9001 attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg cccaaatcac
     9061 atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt tagtgtacat
     9121 aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc cggcgtactg
     9181 ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca ggtaaaaatc
     9241 gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca gtgcgacacc
     9301 cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa tcgcatcttc
     9361 acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt tatagtaacc
     9421 ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat acccttccag
     9481 cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca ccacttcggt
     9541 cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg gaacgggggt
     9601 gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg ggaaacgttc
     9661 gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat gcgacagcca
     9721 gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca gattctgccg
     9781 ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg cggcatcttc
     9841 cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag agacgatttt
     9901 ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt gcttaaactg
     9961 cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt tcagaaacgc
    10021 caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc ccatcgccac
    10081 gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt tagggttttc
    10141 gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag tgatttccag
    10201 atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa gcagcgtcaa
    10261 gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat ctacgcccat
    10321 tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa ctctgacggt
    10381 ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc cttgcgcccc
    10441 gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca agcgcgggca
    10501 acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg caccgatgat
    10561 caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat tgtttcccaa
    10621 ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca acgcgacccg
    10681 cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat cggcggtgat
    10741 tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc cttatccggg
    10801 agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt tcaccgaacc
    10861 cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga ttaagcacag
    10921 tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg ttttgacatt
    10981 agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg caatacgttg
    11041 caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg gcgtgagatc
    11101 gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg gaatcaacgg
    11161 caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg taatgccgat
    11221 ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa agcaatgaag
    11281 aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc gctcgtgggc
    11341 atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta gctgggcaac
    11401 aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga gaccacattc
    11461 accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt cagtcgcagc
    11521 ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg acgaatactg
    11581 acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga tgagtagccc
    11641 attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg caaattgcga
    11701 actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac cgccctgacg
    11761 ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt ttcctcttcc
    11821 cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc aaacagacag
    11881 tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag caacatcccg
    11941 acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc tggcgaggta
    12001 atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac accaaacgcc
    12061 ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac ctgcacccct
    12121 tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa gtaggcgaat
    12181 gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac caggcgacgt
    12241 tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat caccggcgct
    12301 gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg cgaggccacg
    12361 tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc ggataccagg
    12421 tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac cgcgataatg
    12481 cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg agtatcttct
    12541 acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt ttcggttcag
    12601 cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt tccggcgaac
    12661 tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca ttatctttca
    12721 ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg tttgcaacgt
    12781 aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct ttcagttcgg
    12841 gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga aactcctgga
    12901 gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc aacgcgcgaa
    12961 tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga acgacgaggc
    13021 cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat tacacctgct
    13081 ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta tccgcctgct
    13141 tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc tgctttggtt
    13201 catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg gaaccgatgg
    13261 agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc aataactgcc
    13321 aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa caaggccgca
    13381 ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg cgccaaccga
    13441 caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc cattcaggtc
    13501 ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg agaaaataac
    13561 gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta tcaacactgt
    13621 gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt tctggcattt
    13681 tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga ataccgacct
    13741 gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg agctgcggat
    13801 aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac accagttgcg
    13861 gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt acatgtccaa
    13921 acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga atggcaaatt
    13981 cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt agctctgcca
    14041 ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag cgtttatctt
    14101 ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg ttttccgggt
    14161 gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag ctgtcgcgaa
    14221 agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg ttggtgccat
    14281 ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc atcaccatca
    14341 tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg ctgtcttcaa
    14401 aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt tcatcaatca
    14461 aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca tccggcagca
    14521 aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta ataaccggca
    14581 aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc aatcgcgcgg
    14641 tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg tcaaaccacg
    14701 cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc ttgccatcaa
    14761 acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt ggaaaaagat
    14821 cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt tcttgcaggg
    14881 ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc cagaataatg
    14941 agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt tcatcaagtc
    15001 cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct ggcgtcattt
    15061 agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga cggcagccgt
    15121 ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact cttcagtaat
    15181 ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact ttgcgcctcc
    15241 gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg ccagcagttc
    15301 cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca cctgaatatc
    15361 gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc gatcgcgaag
    15421 tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg cccattcgcc
    15481 cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa agccttttac
    15541 ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg cgggtgagcg
    15601 atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt taaaaggcat
    15661 ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct gccgtcagat
    15721 tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg gtatcctgat
    15781 cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg ttcgcgacca
    15841 gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg ctcagcggct
    15901 cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc agcacgcgat
    15961 acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac gaaatggtga
    16021 tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga acatactcaa
    16081 cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc atggattcat
    16141 tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat ttcgctgtca
    16201 ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg ccgctgcaat
    16261 caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca ttcatgacat
    16321 cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt tgttccttcg
    16381 cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc tcaatgcctg
    16441 ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct agatttctac
    16501 ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc atcgcgttga
    16561 taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc attctggctc
    16621 accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc taaaccgcga
    16681 aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt cattgcctgg
    16741 cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc atcgatgaac
    16801 aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc gttgcggctt
    16861 tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag cgtagtcggg
    16921 ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg gcggtcaagc
    16981 gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag agttcgcagc
    17041 cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg caccgacgcg
    17101 atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa atagcgttca
    17161 taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg cagccgcacg
    17221 atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg ggtttcatat
    17281 tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac ctcgccccag
    17341 ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc ttcctgggcc
    17401 atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg gcgttcttgt
    17461 gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg ttcacgtagc
    17521 ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg attgctgtgc
    17581 tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc gctgttaatc
    17641 atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt ggttacttca
    17701 cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc gttattgagt
    17761 aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc cgcgcttaac
    17821 tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc atgttgataa
    17881 ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta aaccatgatt
    17941 gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga attgtgctgt
    18001 acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata caaaaggccg
    18061 aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc gcttccacca
    18121 cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg atctcttgct
    18181 gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga cttgcacaca
    18241 tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc tgccactctt
    18301 ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa cgttcctgac
    18361 ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg ccaatggatt
    18421 tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg aattccagcg
    18481 gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc aggacatcac
    18541 ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag gtgcgaatgc
    18601 ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata ccggtagagc
    18661 agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg cggtgagatg
    18721 ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc gggtcgccag
    18781 ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga tgaaaaacat
    18841 cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag caaaaagaca
    18901 ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca aatgcgttgc
    18961 ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt ctgcaggact
    19021 gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta tagttaacat
    19081 tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac aatctggatt
    19141 tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg acgcgattgt
    19201 ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc atgccagacc
    19261 aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt ttggcgtgca
    19321 tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg ctatcgcacc
    19381 tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca cgttgcttag
    19441 cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc atgccgccag
    19501 ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat tctgctgggg
    19561 tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg cagtggcgtg
    19621 gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg ttgatatcgc
    19681 aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca gcattccgca
    19741 ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag cagagattat
    19801 cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct atcatgccga
    19861 atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg gagagaagcg
    19921 gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg ttttgctgcc
    19981 atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac tccgctattt
    20041 gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg aagacacctt
    20101 agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa cacattcaca
    20161 ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa acattttgtt
    20221 tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac aacaatacta
    20281 tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata ttatttacct
    20341 gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga gcgcatcaat
    20401 aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc tctctaatag
    20461 tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta aattgattga
    20521 caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt tcatggagtt
    20581 tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca ttcaactccg
    20641 cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg atacagaagc
    20701 catcactgtc agttattaat atatactctc caggcatctg attgctaata ctgaatttag
    20761 ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa aaactaaagc
    20821 tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg ctgctgtcgg
    20881 catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg ggggagatgc
    20941 acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg gtgcttcaat
    21001 agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac ttgagcattt
    21061 cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg gtggtccaat
    21121 agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta ttgttgaaaa
    21181 acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata gactgaaaga
    21241 cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata ctcatgagtg
    21301 tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg atggtgacgc
    21361 aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca tctgttatca
    21421 cttaacccat tacaagcccg ctgcc
//
LOCUS       DAZOZD010000053        21250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOZD010000053 DAZOZD010000000
VERSION     DAZOZD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.053"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(10..411)
                     /gene="nikR"
                     /locus_tag="ACHUPB_001594"
     CDS             complement(10..411)
                     /gene="nikR"
                     /locus_tag="ACHUPB_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0758442.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(417..1223)
                     /gene="nikE"
                     /locus_tag="ACHUPB_001595"
     CDS             complement(417..1223)
                     /gene="nikE"
                     /locus_tag="ACHUPB_001595"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0758443.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1220..1984)
                     /gene="nikD"
                     /locus_tag="ACHUPB_001596"
     CDS             complement(1220..1984)
                     /gene="nikD"
                     /locus_tag="ACHUPB_001596"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0758444.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(1984..2817)
                     /gene="nikC"
                     /locus_tag="ACHUPB_001597"
     CDS             complement(1984..2817)
                     /gene="nikC"
                     /locus_tag="ACHUPB_001597"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0758445.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2814..3758)
                     /gene="nikB"
                     /locus_tag="ACHUPB_001598"
     CDS             complement(2814..3758)
                     /gene="nikB"
                     /locus_tag="ACHUPB_001598"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0758446.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3758..5332)
                     /gene="nikA"
                     /locus_tag="ACHUPB_001599"
     CDS             complement(3758..5332)
                     /gene="nikA"
                     /locus_tag="ACHUPB_001599"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0758447.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5443..6030)
                     /gene="acpT"
                     /locus_tag="ACHUPB_001600"
     CDS             complement(5443..6030)
                     /gene="acpT"
                     /locus_tag="ACHUPB_001600"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0758448.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6085..7134)
                     /locus_tag="ACHUPB_001601"
     CDS             complement(6085..7134)
                     /locus_tag="ACHUPB_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0758449.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7266..8483
                     /locus_tag="ACHUPB_001602"
     CDS             7266..8483
                     /locus_tag="ACHUPB_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0758450.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8487..9044)
                     /gene="dcrB"
                     /locus_tag="ACHUPB_001603"
     CDS             complement(8487..9044)
                     /gene="dcrB"
                     /locus_tag="ACHUPB_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0758451.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9117..9782)
                     /locus_tag="ACHUPB_001604"
     CDS             complement(9117..9782)
                     /locus_tag="ACHUPB_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0758452.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            10003..10248
                     /gene="tusA"
                     /locus_tag="ACHUPB_001605"
     CDS             10003..10248
                     /gene="tusA"
                     /locus_tag="ACHUPB_001605"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0758453.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10350..12548)
                     /gene="zntA"
                     /locus_tag="ACHUPB_001606"
     CDS             complement(10350..12548)
                     /gene="zntA"
                     /locus_tag="ACHUPB_001606"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0758454.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12622..13248)
                     /locus_tag="ACHUPB_001607"
     CDS             complement(12622..13248)
                     /locus_tag="ACHUPB_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0758455.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            13389..13748
                     /locus_tag="ACHUPB_001608"
     CDS             13389..13748
                     /locus_tag="ACHUPB_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0758456.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13751..14020)
                     /locus_tag="ACHUPB_001609"
     CDS             complement(13751..14020)
                     /locus_tag="ACHUPB_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0758457.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14010..14606)
                     /gene="rsmD"
                     /locus_tag="ACHUPB_001610"
     CDS             complement(14010..14606)
                     /gene="rsmD"
                     /locus_tag="ACHUPB_001610"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0758458.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14756..16252
                     /gene="ftsY"
                     /locus_tag="ACHUPB_001611"
     CDS             14756..16252
                     /gene="ftsY"
                     /locus_tag="ACHUPB_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0758459.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16255..16923
                     /gene="ftsE"
                     /locus_tag="ACHUPB_001612"
     CDS             16255..16923
                     /gene="ftsE"
                     /locus_tag="ACHUPB_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0758460.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            16916..17974
                     /gene="ftsX"
                     /locus_tag="ACHUPB_001613"
     CDS             16916..17974
                     /gene="ftsX"
                     /locus_tag="ACHUPB_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0758461.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18219..19073
                     /gene="rpoH"
                     /locus_tag="ACHUPB_001614"
     CDS             18219..19073
                     /gene="rpoH"
                     /locus_tag="ACHUPB_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0758462.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19344..20447
                     /gene="livJ"
                     /locus_tag="ACHUPB_001615"
     CDS             19344..20447
                     /gene="livJ"
                     /locus_tag="ACHUPB_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0758463.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20646..21029)
                     /gene="panM"
                     /locus_tag="ACHUPB_001616"
     CDS             complement(20646..21029)
                     /gene="panM"
                     /locus_tag="ACHUPB_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0758464.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5059 a         5611 c         5871 g         4709 t
ORIGIN      
        1 agaaataact caatcttcct tcggcaagca ctgcaaatgc ccatgccgca cgccgcgctg
       61 ggcgataacg tcatcggcaa aatgctgcac gtcgcccata tcacctttca gcacggcgat
      121 ttccagacag tcgtcgtgat tgatatgcac atgcagcgtg gcgacggaga gatcgtggtg
      181 atggtgctgg gtggagacaa tgcggctggc taagtcgcgt ttttcgtgtt catacacata
      241 ggagagcacc gcgaaacctt gcgtgccgtg ctgctgagtg gcctcctggg ccagggcgct
      301 acgcagaata tcgcggatag cttcggaacg gttgttataa ccacgacgct ggctcaggct
      361 gtccagcgtc tccagtaaat cgtcatcaag cgtgatggtg actcgttgca tttgcgttaa
      421 accttttctg tggtgcgacg gcgcacgggg aatgcgggta ataccgcgtt ttgtagcaca
      481 cgtccggcgt cagaggaaaa ggttaattta tctcccacca cctgggtttc gacgatttgt
      541 ccgttgtcca tcaccatcac ccgctgacag aagcgttcca ccaggcgtaa gtcgtgggtg
      601 atgaacaggc aggcggtgcc aaactgttgt tgtagctttt tcagcaggcg aatgacgccc
      661 gcctgtaaca cgagatcaag gttagaaacg gcttcatcca gaatcagtag tttcggttcg
      721 accgccagcg cgcgagccag gcagacgcgc tggagctggc cgccgcttaa ctgcggtggg
      781 cgtttgtcga gaacgctgtc atcgagatcg accgccttca gcatttcgct ggcgcgcgcc
      841 agttgttcgg attttttcag tgatagcagg tggcgcatcg gttcacgcag gatctcgcgc
      901 acggttttgc gcggattcac ggcgctgatg gagtcctgaa ataccatctg aatatcgcgg
      961 cggaacgctt tacgctgggc gcgattgagt ttcgccagcg gttcgccacg ccagctaata
     1021 ttcccctgcg cgggcgattc taaacccacc agcaaccgcg cgagggtact tttcccgcag
     1081 ccgctgcgcc ccagcagggc gacggtttcg ccgcttttca gggtcaggga aacgttattc
     1141 agcaccgcct gatgttgatg ttttccgtta aatccaccgt gggcatagtg atgggaaagg
     1201 ccggagacgt taagtaaagt catgatgcca gctccatacc gtagagggcg agatgagcgg
     1261 aaaccaggct gcgcgttacc gcatgtttgg gggcgttaaa cagcgtttct acatcgccct
     1321 gttcgacaat tttaccttgt gacatcaccg ccacgtcatc cgccagacgc gccacaacgc
     1381 ccatatcatg ggtgaccagc agcattcccg gcgcttgttt ttgcataatg ctttccagca
     1441 gatcgaggat gcgcgcctgt gctaccacgt cgaggtcggt ggtcggttca tcggcgatga
     1501 taaacggtga ttcacacagc accgccatcg caatcatcat gcgctgcaac atgccgccgc
     1561 tcatctcgaa cgggtacagc ttcagcacgc gcgcggcgtt ttccagcccc accgcttcta
     1621 tggcagcggt aagcgtagcg tcatcggcgg gtttccctaa cgccaggcag gtttcccgcg
     1681 cgtgggtgtg catggtgtgt agcggattaa aggcgctgcg cgggttctgc atgatggtgg
     1741 caattttgat gccgcgcagg gcgcagggat aaaccgcttt gccatcggct aaaatttccc
     1801 ccgccgtctg gcgaacgcca gcgggcaaaa tgcccagcgt cgcggcgcag gttaatgatt
     1861 tcccgctgcc gctaccgccg actaacgcca gcacgcgccc gcgttgcagg gttaacgata
     1921 caccgtgtac cagcggctgc gcggcctgta gcgcgatatt acgtagttca atctgttgcg
     1981 gcattagtgt gcgtgctccg tcaccagatg aggatccaga tgatcgcgca gtgcgtcacc
     2041 caccaggtta aaggccatca cgctgataaa cagcgccagc cccggccaga acatttgcag
     2101 cggctgggtc cagatatact ggcgcgcgtc gttaatcatc acgccccatt cggcggtcgg
     2161 cgcggtcaca ccgaggccaa ggaaagacat ccccgcgacg tgcagcatca tatggccgat
     2221 atccagcgtt gccagcacca gcagcgaagg gatcaccgcg cctgccagat gatcgacaaa
     2281 cacccgcaca tggcccgcac cggaaagccg tgacgccagc acaaactcgc gttggcgtag
     2341 tgaaatcacc aagctgcgca ccatgcgtgc ataccacgcc cagtgcgaca gggcgatggc
     2401 gataattacg ttggtcagcc cggtgccgag cacgccaacc ataaagaacg acagaatcga
     2461 ggtcgggaag gtcataaaca tatcggcgac gcgcatggtg gcctgatcaa cgcgcccgcc
     2521 aatcaacccg gcgctgccgc caataaccag ccctaatgtc agcaccagca gcaggcaggc
     2581 cattaccgaa ccgagcgaca cgcgggtcgc tgccatcagc cgcgagaaaa tatcgcgacc
     2641 taagtgatcg gtgcccagcc agtgctgcgc atccggcgaa agcaggcgcg acggcaaatc
     2701 aatcgcctgg gggtcatacg gcagccacca ctggctggtg agcgcaatca gcgccagcag
     2761 ggcgataatg atcagcgcca ggcgtaccga ccagcgggaa gagaggaaaa agttcacgcg
     2821 tgcgctcctt catgacgacg aatgcgcggg tccagcgcgg cgttgagcaa atcgacaatc
     2881 aaattacaga ccacaaaaac caccaccatc atcagcgtaa agcactgaat caccggatag
     2941 tcacggttaa aaatcgccga caccgcatag cgcccgacgc ccggccaggc aaagatgttt
     3001 tcgataatca tcgtcccgcc aatcagttcg ccgatgtgca tccccacggc ggtgatcatc
     3061 ggcagcgagg cattgcgcag aatatggcgg cgttcggtct gtttgtcgtt caggccgcgc
     3121 agacgcgccc aagtgacgtg acgctgaccg gcgacgtcca gcatactggc gcgcagcagt
     3181 cgcgcgttaa tcgccagcga cataaaggca atggaaaccg caggcaaaat gatgtgctgc
     3241 cagccgccgt aacccatcgc gggtagccat tgcagataca ccgaaaacgc catcaccagt
     3301 aaaaacgcca gccagaagtt aggcatcgac acgccaagaa acgcgatgaa acgtacggcg
     3361 aaatccggca gacggtcgcg atggcgcgcc gcccagatac cgagcggtac ggaagtgagc
     3421 agaattaata ccagcgccgc acctgcaagt tccagcgtgg cgggcaggaa gttcagcata
     3481 tcgtccagca ccgggcgttg gctggcgaat gagataccaa agtcaagatg cagcgccttc
     3541 cacaaccagg taccgtactg gacgtacagc ggctgatcca gcccgagcat ggtgcgggta
     3601 gaggccagca tctccggcgt cggcggcagg ttagataaac gcagataatc gagcgccggg
     3661 tcgccggtac cgaggcgcag catcagaaaa atgatcaccg aggcggcgag caccatcggg
     3721 atcagcagca gaaagcggcg taatacgtaa cgcaacatta aggtttcacc ggtttaatct
     3781 gttcgaacgg aatttcggtg gcgatcggcg cgtaggggat attacccagc tccggttttg
     3841 ataccaccat catcgagatg tagctgatag gcagataaac cgcctcgtca tgcagacggg
     3901 tcaaaatgtc gcgatacagc gcctgacgtt gcgtttcgtc atgggtcgcc agcacttcgc
     3961 cgatctcttt atcaatcagc ggcttgtcgg ctaatccttg ctgtgcctgg aagtcagcgt
     4021 gcgacggtac gcgcattgaa ctcaggaagg cgtgtggatc gtatggcgcg ccccaggtgc
     4081 ggtggaaaat catgccaaaa cgaccgtcgc gctgacgagc atagatactg ctctcttctt
     4141 cgccaatcag cgagacatct gcgccaatct ggcgcatatc agcctgaatg atttccgcca
     4201 tcgatttgct taacgcatcg gtgccgatga acgacagttc aatgcgcagc ggctgaccat
     4261 ttttctcgcg gatgtctttg cccgcaggca gcgtccaacc ggctttttcc agcaactctt
     4321 tcgctttctg cggatcgtac tggcgcggtt tcaggccgag attagcgtaa ggcacagaag
     4381 gggcaaacag ggtgtcggcg acctgcgggg tgccatataa cgcgttatca atcagcgatt
     4441 ttttgtttac cgcgtaatta agagcttcac gtactgccag ctcgttggtg ggggctttgg
     4501 cggtattgag cgccagcatc acggtttcga tcggctgtga cagttgggtg tggtaagccg
     4561 gattctggct aaagcgggcg aaggtatcga gcggtaataa cccttcgttt ccgtacagca
     4621 ggtcgatatc gccagtttca aacgccaccg cgcgggtagt cgggtccggg atgacgttaa
     4681 aggtgatctt tttaatcgct ggcttttcgc cccagtagtt ttcgttacgg acgaagacat
     4741 cgtactgatt cagtttcgat tcctgcaaaa tccacggtcc ggtgccaatc ggcgctttaa
     4801 ttccgttcat ggtttcatgg tttttaaact gcgagggagc gataaaacgg aaaggacggg
     4861 gcagggccag ttcttgcagg aaaggatagt aggcgctttt cagggtaatt tgcagctctg
     4921 ttttactgag tgctttaaca tcaacaatct ggtttgccag ctccagccag gcgtgacgtt
     4981 gacggttatc gagcactgcg cggaagtttt ctgccgccgc ctcggcatcg aacggttcac
     5041 cgttggagaa tttcacgtca tcacgcaggg tgaaggtcca ggttttacca tcttctgaat
     5101 gagtccagct ttttgccagc cacgggatca ccgaaccgtc tgcctgatat ttcaccaatg
     5161 gttcataaac catgctctgg gcgaacatct ggttaggcgt gtaaaggtgc gggtttagtg
     5221 gcccgacatt caccggccag gcggtagtga tttcatctgg tgcagcggca tggacgataa
     5281 aagacgcaca agccagcagc gcaaatagag tgcggcggag tgtggagagc atggtaaccc
     5341 caatggatta aaatagatgg cggaaataag tatgacgatt ttaagtattc gtcatactga
     5401 ttacctgttc tggatcaata gatgggcagt cgggtgggcg gatcagttaa ctgaatcgat
     5461 ccattgcaca ctgtcggcgg tgagcgtaaa gggggtgggg gtgcagatcg caaggcttaa
     5521 attttctagc tgacaatggc tgactgacag cgaggagtga taggtgctat ctacgctgac
     5581 gatttgccag gcgctgccgc cacgctgttt aacgatggct tctttgcgcg tccagatgcg
     5641 ccagaacatt tccagttgct gatcaggatg cacggcgtcc atttcagcgt gttccccgag
     5701 gctgaacacg gcgttcgcca gccagcgcca gttggcgcgc gggcgaatca cttcgatatc
     5761 gcagccgact tcgccttcat cgctcaacag cagagcgata tcgtcaccgc tatggcttaa
     5821 gttgaaccat agcggcgttt ccggcgcgaa tgcaggtttg ccttgttcgc catagatgat
     5881 ctccggtagc ggggaaagcg tgtgcgaaag caatgcacgc cccgccagcc agcgttcgcg
     5941 tcgtggacct tgcggtgctt gctcgcgtaa acccggtggc agtggagctg cgcttaaggt
     6001 cgaaactttc cccagaacta tccgatacat atcagggcca acgtttaatg gaaaatgaaa
     6061 gtgcgtatcg tatcacttgt cgcctcatcc cggtaaccga cttttcggtc tgcctggccc
     6121 cagtaaaatc gccagtttgc taccgccttt ggtggtttcc atccagattt tacacacact
     6181 ggttaatggc accgaaagta gcatccctac cgggccgagc agccatcccc aaattaacaa
     6241 tgaaagaaat accaccatgg tggacatccc caggcgatgg cccatcatcc gtggttctaa
     6301 aatattgccg atgaccatat ggaccactaa aaacaatgcg ccgaccagaa tacattcgta
     6361 aacaccatta aacagcagca cctgaatcat tggcggtacg gcggaaatta ccgcgccgat
     6421 attgggcacg tagttgagca aaaacgccag tactgcccac atcagcgcaa actgcacccc
     6481 catcagctcc agccccagcc agacgatgac acctgtccat aaactgagta gcgtcttcaa
     6541 tgcaagatag tgcgaaacgc ctttaagtgc gcggtgtaat cctgcgatgt gaatctgtgg
     6601 gttattcagc gcaaaacgca ttttgtaagg gacgtggcgc acttcaaaca gcataaaaac
     6661 tacggtcatc accagcaaaa gcacgctcgc cattgccccg gaaagcccgg tcattagcgc
     6721 tgtggtgaag gtaaccactt tttccgagtc catccgctgc agcattcgct ccggcgacat
     6781 atgcaaatta agaaaaggca acatctcctg caatttaaaa agtttgcgcg tcagctcctt
     6841 attaaacttc ggcagcatag agataaattc gttaaacgat gccgccagta cgccgaccag
     6901 cgcggttagt gcgatcagca tcaccaccac tacaatcgta atggcaacgg ggcgttgtac
     6961 tccccgacga ataaaccagg taacgagcgg gttgaggacg atggcaaaaa acagcgccag
     7021 taacagctgc acaatgatat ctgccgctgc gtgaatgccc gcgaggatca ctaccagcga
     7081 ggccagcttg agcagaatgt gcatgcccgt tttatcgggt tgagggattt ccattggggc
     7141 ttccttgtga ctttttgtat taagtgtagc gggagtcacg ccagcaatat tctgattctt
     7201 actgctgatt ttcacgtcag cacgtggtaa aaatgaaaca ctgttgtaaa aatgtggtga
     7261 tcctcatgcc cgaacccgta gccgaacccg cgctaaacgg attgcgcctg aatttgcgca
     7321 ttgtctccat tgtcatgttt aacttcgcca gctacctcac catcgggttg ccgctcgctg
     7381 tattaccggg ctatgtccat gatgtgatgg gctttagcgc tttctgggca ggattggtta
     7441 tcagcctgca atatttcgcc accttgctga gccgtcctca tgccggacgt tacgccgatt
     7501 tgctgggccc caaaaagatt gtcgtcttcg gtttatgcgg ctgctttttg agcggtctgg
     7561 gatatctgac ggcaggatta accgccagtc tgcccgtcat cagcctgtta ttactttgcc
     7621 tgggacgcgt gatccttggg attgggcaaa gttttgccgg aacgggatcg accctgtggg
     7681 gcgttggcgt ggttggctcg ctgcatatcg ggcgagtgat ttcgtggaac ggcattgtca
     7741 attacggggc gatggcgatg ggtgcgccgt taggcgtcgt gttttatcac tggggcggct
     7801 tgcaggcgtt agcgttaatc attatgggcg tggcgctggt ggccattttg ttggcgatcc
     7861 cgcgtccgac ggtaaaagcc agtaaaggca aaccactgcc gtttcgcgcg gtgcttgggc
     7921 gcgtctggct gtacggtatg gcactggcac tggcttctgc cggatttggc gtcatcgcca
     7981 cctttatcac gctgttttat gacgctaaag gttgggacgg tgcggctttc gcgctgacgc
     8041 tgtttagctg tgcgtttgtc ggtacgcgtt tgttattccc taacggcatt aaccgtatcg
     8101 gcggcttaaa cgtggcgatg atttgcttta gcgttgagat aatcggcctg ctactggttg
     8161 gcgtggcgac tatgccgtgg atggcgaaag tcggcgtctt actggcgggg gcagggtttt
     8221 cgctggtgtt cccggcattg ggcgtagtgg cggtaaaagc ggttccgcag caaaatcagg
     8281 gggcggcgct ggcaacttac accgtattta tggatttatc gcttggcgtg accggaccac
     8341 tggctgggct ggtgatgagc tgggcgggcg taccggtgat ttatctggcg gcggcgggac
     8401 tggtcgcaat cgcgttatta ctgacgtggc gattaaaaaa acggcctccg gaacacgtcc
     8461 ctgaggccgc ctcatcatct taaaacttac tgaataacca gcgtattaat gatgttttct
     8521 gcggtggtct gcgctttttg ctgatcgtca gctggcagag taatttgcat ggtcagcagt
     8581 tgattaccca cgttacccag aataacggaa gagtacgccg tctggccttt cgcggagata
     8641 atactgtcta actgctgcat tttgtgacct ttcagctcaa tggctttgtt ggttaccact
     8701 tgcagctgcg gatcgcggct acgttgctga tcttccagac gcttcgccaa caccgccaga
     8761 tcttctttcg gatcatcgcc catgatgacg atgactgctt tctgcccggt ggcgtcggac
     8821 cagacatgca tgttattggc ctgcgttccc agcttaccgc tctggtcggt catatccgct
     8881 ggcagcgaga aacttaactt gccatcaagc aggttgacgg gattcccggt agcgttactt
     8941 tccgcgaccg aaccctgcgc cgtagcgtta gtgtctttat catcacaggc cgcaagcccc
     9001 ataaccagca ggccaattcc gacatattta accagattgc gcattgactt cttcctttcg
     9061 ataaacggcc ataacggctc attcatccat cttatcacaa ctctgataac gaacctttaa
     9121 ctcgcctgca aagcgttgat ttcggattta tctgccagtc ttttcaacag aatattgagt
     9181 aatacgccat acattggcag gaagaaaacg atactgatta acactttgaa acagtaatcg
     9241 accagcgcga tttccatcca gtgttcagcc ataaaggcat ccgggctacg ccagaaggca
     9301 atgaagaaaa aggccagcgt gtcgctgacg ttaccgaaca gtgtggacgc tgtcggtgcc
     9361 agccaccagc ggcgactctg acgcaggcgg ttaaaaacgt gcacgtcgag gatctgtccc
     9421 agcgcgtagg ccatgaaact ggcggtggcg atacgggcga caaacaggtt gaagtgggcg
     9481 agtgcgccga atccctgcca ggaacccata tagaatagcg acgagatgac gtaggagatc
     9541 agcaacgccg ggatcatcac cgcaaagatg atacgacggg ccagcggtgc gccaaaaata
     9601 cgcacggtca ggtcggtagc aaggaaaata aacgggaagc taaacgcgcc ccaggtggta
     9661 tggaaaccca aaatggagac gggaagctga accagatagt tactggaggt gatcaccagc
     9721 agatgaaata acgataacca gaacaacgcc ttatagcgtt gagtttgcga gaaaacgttc
     9781 atattgtacc tttttgatta accattgggg tgagggaacc caatacgtac gacacgtctg
     9841 ttaccttaac ataataacgt gcttaccctt ttttcgagcc gccgcatgat actgctttac
     9901 gttgacaatg caatggttaa ttttcacgca atcgttaacc tggtttgctt acggactcac
     9961 agggcgtaaa ctagcgccgt ttttttatgt gatgagaaga aaatgaccga tctcttttcc
    10021 agccctgacc acacactcga cgcacttggc ctgcgctgcc cggaacccgt aatgatggtg
    10081 cgcaaaactg tgcgcaatat gcagcctggc gaaacgttgc tgattatcgc cgacgatccg
    10141 gccactaccc gcgatattcc tgggttttgt acctttatgg aacacgaact ggttgcgaaa
    10201 gagacggatg gactgcctta tcgttatttg attcgtaaag gcggttgatg gggaaagatt
    10261 ggcttcgatg ccgccttttc ccctcaccct aaccctctcc ccagaggggc gaggggaccg
    10321 atcgcgctca atgttgcgat cggtttgcct tatctcctgc gcaacaatct taacgcattc
    10381 gctgtcacca gcaccgtcgc acccgtatct gccagcaccg ccagccacag gccggtcatc
    10441 cctaacagcg tggtgacgag gaagatccct ttcagcccca gcgcaatggt gatgttctgg
    10501 cggatattgg cgtgagtggc gcgtgccagt tcaatcattt gcaccaggcc gcgcaggtgg
    10561 ttatgggtta atgctgcatc agcggtttcc agcgccacgt ctgtgccact gcccattgca
    10621 atcccaatgg cggcagcttt catcgctggc gcgtcgttaa taccatcacc gaccatcgcc
    10681 aggggcgcat gttgattcag cttggtcacc gctttgactt tatcttccgg caataggccc
    10741 gctttaaact ccagccccag ctccccggcg attgccgccg ctgcacgtgg attatcgccg
    10801 gtgaggatca ctcctttgac ccccagcgcg ttcagttcac tgatggcagt tgcagcatcg
    10861 gcgcgcaggg tatcctgcaa tgcaatgata cccagcacgt catcattacg tactaccagc
    10921 acaaccgttt gcccggcgct ttccagttca ttaatcaaac cagcaaatgc atcagcggga
    10981 tgtttcccgg cagcgcaaat caatacgcgc tcaccgttaa cctgcgcttc aatgccagac
    11041 ccgaccagcg cccgctgtga ttcggcggtg ggaatggcga gttcagcaac ctgtgcttcg
    11101 cgtacgatgg cttgcgccag tggatgcgtc gcgccttgct cgaccgccgc cgccagtgtc
    11161 agcagttcag attcactaat acccgttgcc ggatgaatcg cggtaacgcg cggtttaccg
    11221 acggtcagcg taccggtttt atcaaacgcc acctgagtaa cacgacctag ttgttccagc
    11281 gccgctccgc ctttaatcaa cgccccacga cgcgctgccg ccgccagccc ggaggtaatc
    11341 gccgcaggcg ttgagataac taacgcacac gggcagccaa tcagcagcag cgtcagccct
    11401 ttataaatcc actcctgcca gctggcggca aacagcagcg gtggcaccag cgtcaccagc
    11461 agagcgacgg ccataatcgc gggcgtatag atacggctga aacggtcgat aaaccgctca
    11521 atgggggcgc gacgctcttc ggcttcttcg atcagtttca gaatccggtc aatggcgctg
    11581 gcacccggtt ctgacagcac ttccagcgtt accagacggt ctacgctggt ggctcctgca
    11641 ggaactttat cgcccgtcgc gcgctccacc ggaatggatt ctccggtcag ggcgctttca
    11701 tcaaaactgg caaacggtga gagcagttta ccgtcggcag gcaaacgccc acctgcggcg
    11761 acttcaatca catcgccagg ccgcaggctg ttaatcgcca cctcttcccg ctcaccgtta
    11821 cgcaggcgcg tggcggtttc tggtttcagc gccattaacg cgctaacgcc ctgacgcgcg
    11881 cggctggcgg cccagccttc cagtcgttca ccaatcaaaa acagcagcaa caccatcgca
    11941 gcttcagccg ttgcgccaat aaacagtgca ccaatagcgg ctacgctcat taaggtttca
    12001 atggcgaagt agctgccgga tttgatcagc cgtaatgcct gacgagcaat cgggtacagc
    12061 ccaaccagcg tggtcgcgat aaacgccagt tgcccgaacg gatgattgaa ctgctccaga
    12121 ccccagctga ttgccatcat cacgattagt gtaatcagcg gcagattctc tttcaggcgc
    12181 gatgcttgtg gctcgtcggc ggcctgctca tcgcgcaggg aatagcccgc tttttgcacc
    12241 gcagattcaa cttgtgcacg aatgtcattg tcggcatcaa ccaccagttt ttcggtggcg
    12301 aacaacacct gcacctgatt cacacctgca agctggcgca cggcattttc taccttgcgc
    12361 gcacaggcgg cgcagtccat gccactgact ttccagctat agcgggtgcc ggagacgttt
    12421 tcagagagcg ttggcgagct ggagcatgcg ccgtcgcagc aacagtcgtt ggcgttctgt
    12481 accgtggtta gcggtttgaa cgcagcaaat tgaggggctt tcttgccgtg attgtcaggt
    12541 gtcgacatgg catactccgg ttaagttttt tctcattaac cgaaggatac actctggagt
    12601 cgactccaga gtcaagtttt atcagagata cagtgagcgg atgatgagga agtgaccggc
    12661 aaagtagcag gccgcagcaa tcgcgttatc cgcacggaag cgtcggcgat agtggctccc
    12721 cagccagaca aagttactga taaacagcaa cgaagcgcca acaaacgcag agagcgccgg
    12781 agcggtcgga cggaagaacc acagttcacc tgccagccac accatcacca gcgtcatgcc
    12841 gataaacgtg cagataggcc aacgatactc ttccagccgc gtccagataa tcgccagtaa
    12901 cagcgcaccc agaaccagca acaccagtgg cagaggccag aagaaagaga gcgtcatctg
    12961 actggcgaaa tagatggtgt acagcaggtg cgagaggaaa aacgcgccga tggcgtacat
    13021 cagacgttga cgtggcaaca gggttagcgc atcgcccagc agtgaggcgc acagccctgc
    13081 cagcaccaga tagctaatgg cgtcgaacat cggcgcttgc caggccagta acagcaggag
    13141 aagaagggtt aacggtttaa acacccagcg ttgccaggtt ggcccacgat acgatgcatc
    13201 cacagatagc catgcggaaa gacagacagc gataaacgac caaagcatct tagttccttg
    13261 aattcgttat tttttcgtaa gcggaatgcc tacggtcttg cttccagttt agtggcgagg
    13321 gtggacggat gacaacacca taactggcag ggtatgctta tttccgcatt ttccgatgag
    13381 ggataaagat gagcaaacca cctcttttct ttattgttat cattggctta attgtcgtcg
    13441 ccgcatcgtt tcgttttatg caacagcgac gggaaaaagc tgataatgat atggctccgc
    13501 tccagcaaaa gctggtggtg gtgagcaaca agcgggaaaa accgattaac gatcgccgtt
    13561 cgcgccagca ggaagtgact ccggcaggca ccagtatgcg ctacgaggca agcttcaaac
    13621 cgcaaagcgg aggaatggag cagacgtttc gcctcgacgc ccagcagtac cacgccctga
    13681 cagtgggcga taaaggtacg ctgagctata aaggaacgcg ctttgttagc tttgttggcg
    13741 aacaataagg ttatttttta actttaaatt tcttctgcca gaccagcagt tcaaacacgc
    13801 cgaaaaggaa aatccgtacc ttttcggcgg tggtcatctg cgggccatct ttcggtaaag
    13861 tggatttcaa tagcgccagc tgcataccat gcatcagcac ggtaaaaatc agcgccacgt
    13921 taacgaaaat atttagcggg cgcgggaagg gatgcaccag attgaggatt aaaaatcccc
    13981 aaacgcagag cattaacaaa cgaccaatat taatcagcat cactttctcc ttgtgcttcg
    14041 cgttgataca gccgataagc cacctgaccc gccacttttt cccgatgtaa cgaccagttt
    14101 gccggaacgg tgggcagacc gttttcgact tcgctttcga cataaatcag ggcttcgtca
    14161 gccagccagc cgttatcttc cagtaaattt atcgtctctt ctaacaagcc acggcggaac
    14221 ggtggatcga caaacacgat attatgcggt gtgccttttt gcgccaggaa tgacatcgcg
    14281 ttgctgttca ccacgcgtgc attgcctgct tttagtgtcg ccagattctt aattaactgc
    14341 tgagaaactg cacgatccat ctcaattaac gtggcccccg cagcgtagcg cgataacgct
    14401 tccagcccca gcgcgccgct cccggcgaag caatccagac attgggcgtc aacaatgacc
    14461 ggagccagcc agttaaacaa cgtttcgcgt acgcggtcgg tggtggggcg cagacctggg
    14521 ctatcaggaa ccgggagttt acggcctcgc cactgcccgc caataatgcg gatttggccg
    14581 ctgccggaat gattcggttt tttcatgata aattgctcaa tccgccagat aacacatact
    14641 tgcaggcggt gatgtgaatt aagttaagtg atagactatt tcatcatttt tttagctgct
    14701 atgtacatag cgttaacgct gtgccatgaa gcaacagcga ggagtgtagt cgcaaatggc
    14761 gaaagaaaaa aaacgtggct ttttttcctg gctgggcttt ggtcaaaaag agcagacccc
    14821 ggaaaaagag acagaagttc agaatgaaca accggttgta gaagaaatcg ttcaggcgca
    14881 agagcctgtg aaggcttcgg aacaagccgt tgaagagcag ccgcaggcgc atactgaagc
    14941 cgaggcggaa acttttgctg ccgacgttgt ggaagtcact gaacaggttg ttgaaagtga
    15001 aaaagcgcag cctgaagcgg aagttgttgc acagccggaa ccggtcgtag aagaaacgcc
    15061 ggagccagtg gctatcgaac gtgaagagct gccgttgccg gaagacgtca acgccgaaga
    15121 ggtttcgcca gaagagtggc aggctgaagc ggaaaccgta gagattgtca aagcggcgga
    15181 agaagaagcg gctaaagaag aaatcaccga cgaagagctg gaagcacagg cgctggcagc
    15241 cgaagcagca gaagaggcgg tgatggtggt tcctccggca gaagaagagc agccggtgga
    15301 agaaatcgct caggagcagg aaaaaccaac caaagaaggt ttcttcgcgc gcctgaaacg
    15361 cagcctgtta aaaaccaaag aaaatctcgg ttccggattt atcagcctgt tccgcggtaa
    15421 aaaaatcgac gatgatctgt ttgaggagct ggaagagcag cttttgatcg ccgatgtggg
    15481 cgtggaaacc acacgtaaaa ttatcaccaa tctgacggaa ggcgcatccc gcaagcagct
    15541 tcgtgatgcc gaggcgctct atggcctgct gaaagaagag atgggtgaga ttctggcgaa
    15601 agtcgatgag ccgctgaatg ttgaaggtaa aacgccgttc gtgatcctga tggtgggcgt
    15661 caacggtgtg ggtaaaacca cgacgattgg taagctggca cgtcagtttg agcagcaggg
    15721 taaatcggtg atgctggcgg cgggcgatac tttccgtgca gcagcggttg aacagcttca
    15781 ggtctggggt cagcgcaaca atattccggt gattgcccag cataccgggg cggattccgc
    15841 ctctgttatc ttcgacgcca ttcaggcagc taaagcgcgt aatatcgacg tcctgattgc
    15901 cgataccgcc ggacgcctgc agaacaaatc gcacctgatg gaagagttga agaaaatcgt
    15961 ccgcgtgatg aagaaactcg acgttgaagc gccgcatgaa gttatgctga ctattgatgc
    16021 cagcaccggg cagaacgcgg taagccaggc caaactgttc cacgaagctg ttggcttaac
    16081 cggcatcacg ctaacgaaac tggacggcac ggcgaaaggc ggggtaattt tctcggtggc
    16141 tgaccagttt ggtatcccta tccgctacat tggtgtcggc gaacgtattg aggatttgcg
    16201 tccgtttaag gcggacgact ttatagaggc actttttgcc cgagaggatt aacaatgatt
    16261 cgctttgaac atgtcagcaa ggcttatctc ggtgggagac aggcgctgca gggcgttacg
    16321 ttccatatgc agccgggtga gatggcgttt ctgaccggtc attccggcgc agggaaaagt
    16381 accctcctga agctgatctg tgggattgag cggcccagcg ccgggaaaat ctggtttagc
    16441 ggccatgaca tcacgcgtct gaaaaaccgt gaagttccgt ttctgcgccg ccagattggc
    16501 atgattttcc aggatcacca tctactgatg gaccgtactg tctacgataa cgtggcgatc
    16561 ccgctgatta tcgctggtgc cagcggtgac gatattcgtc gccgggtgtc ggcggcactg
    16621 gataaagtcg ggctactgga caaagcgaag aacttcccta ttcagctttc cggcggtgaa
    16681 caacagcgtg ttggcattgc ccgcgcggtg gtgaataagc ccgcggtact gctggcggac
    16741 gaaccgaccg gtaacctgga cgacgcgttg tcggaaggga ttctacgtct gtttgaagag
    16801 tttaaccgcg ttggggtaac cgtattgatg gcaacgcacg acatcaacct gatttcacgg
    16861 cgttcccatc gcatgctcac cctgagcgat ggtcacttgc atggaggcgt gggccatgaa
    16921 taagcgcgat gcaatcaatc atattcggca gtttggtggg cgtctggatc gcttccgtaa
    16981 atcggtcggc ggctcaggcg acggcggtcg taacgcaccg aaacgcgcga aatcctcgcc
    17041 aaaaccggta aatcgcaaaa ccaacgtttt caacgaacag gtgcgctatg ccttccacgg
    17101 cgcattgcag gatctgaaaa gcaaaccgtt cgccacgttt ttaacggtga tggttatcgc
    17161 catctccctg acgctgccca gcgtctgtta tatggtgtac aaaaacgtta atcaggcggc
    17221 gacgcagtat tatccgtcac cgcaaatcac tgtttacttg caaaaaacgc tggacgatga
    17281 cgccgctgcg ggcgtggtgg cacagttgca ggccgagcaa ggcgtggaga aagtgaacta
    17341 tctttctcgt gaagacgcac tgggcgagtt ccgtaactgg tctggttttg gtggtgcgct
    17401 ggatatgctg gaagaaaacc cgcttccggc agtggcggtg gtgatcccga aactcgattt
    17461 tcaggggacg gaatcactca atacgctgcg tgatcgtatc acgcagatta acggcattga
    17521 cgaagtgcgg atggatgaca gctggtttgc ccgtctggcg gcgttgaccg ggctggtcgg
    17581 gcgcgtttcg gcgatgatcg gcgtgttgat ggtggcggca gtgttcctcg tcatcggcaa
    17641 cagcgtacgt ctgagcatct ttgctcgccg tgactccatc aacgtgcaga aactgattgg
    17701 tgcgacggat ggtttcatcc ttcgcccgtt cctgtatggt ggcgcactgc tgggattttc
    17761 tggcgcattg ttgtcattaa ttttgtcaga aattctggtg ctgcgattgt catcggcggt
    17821 tgcggaagtg gcacaggttt tcggaacgaa gtttgatatc aatgatttat cgttcgatga
    17881 atgcctgcta ttgctgctgg tatgctcgat gattggctgg gtggcagcgt ggcttgccac
    17941 ggtacaacat ttacgccact ttacgcctga ataataaaag cgtgttatac tctttccctg
    18001 caatgggttc cgtagcaggg aaagagatcc cgttgtctct tcccggtatt tcatctctat
    18061 gtcacatttt gtgcgtaatt tattcacaag cttgcattga acttgtggat aaaatcactg
    18121 tctgataaaa cagtggatga taacctcgtt gctcttaagc tctggcacag ttgttgctac
    18181 cactgaagcg ccagaagata tcgattgaga ggatttgaat gactgacaaa atgcaaagtt
    18241 tagctttagc cccagttggc aacctggatt cctacatccg ggcagctaac gcgtggccga
    18301 tgttgtcggc tgacgaggag cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg
    18361 aagcagctaa aacgctgatc ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt
    18421 atgcgggcta tggcctgcca caggcggatt tgattcagga aggtaacatc ggcctgatga
    18481 aagcagtgcg ccgtttcaac ccggaagtgg gtgtgcgcct ggtctccttc gccgttcact
    18541 ggatcaaagc agagatccac gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga
    18601 ccaccaaagc gcagcgcaaa ctgttcttca acctgcgtaa aaccaagcag cgtctgggct
    18661 ggtttaacca ggatgaagtc gaaatggtgg cccgtgaact gggcgtaacc agcaaagacg
    18721 ttcgtgagat ggaatcacgt atggcggcac aggacatgac ctttgacctg tcttccgacg
    18781 acgattccga cagccagccg atggcaccgg tgctctatct gcaggataaa tcatctaact
    18841 ttgccgacgg catcgaagat gataactggg aagagcaggc ggcaaaccgt ctgaccgacg
    18901 cgatgcaagg tctggacgaa cgcagccagg acatcatccg cgcgcgctgg ctggacgaag
    18961 acaacaagtc cacgttgcag gaactggctg accgttacgg tgtttccgct gaacgtgtac
    19021 gccagctgga aaagaacgcg atgaaaaaac tgcgcgctgc tatagaagcg taatttccgc
    19081 tattaagcag agaaccctgg atgcgagtcc ggggtttttg ttttttgggc ctctacaata
    19141 atcaatttcc cctccggcaa aacgccaatc cccacgcaga ttgttaataa actgtcaaaa
    19201 tagctattcc aatatcataa aaatcgggta tgttttagca gagtatgctg ctaaagcacg
    19261 ggtagccatg cataaaacga aataaagtgc tgaacaacaa catcacaaca cacgtaataa
    19321 ccagaagaat ggggattctc aggatgaaca taaagggtaa agcgttactg gcaggatgta
    19381 tcgcgctggc attcagcaat atggctctgg cagaagatat taaagtcgcg gtcgtgggcg
    19441 caatgtccgg tccggttgcg cagtacggtg accaagagtt taccggcgca gagcaggcgg
    19501 ttgcggatat caacgctaaa ggcggcatta aaggcaacaa actgcaaatc gtaaaatatg
    19561 acgatgcctg tgacccgaaa caggcggttg cggtggcgaa caaagtcgtt aacgacggca
    19621 ttaaatatgt gattggtcac ctctgttctt catcaacgca gcctgcgtct gacatctacg
    19681 aagacgaagg cattttaatg atcaccccag cggcaaccgc gccggagctg accgcccgtg
    19741 gctatcagct gatcctgcgc accaccggcc tggactccga ccaggggccg acggcggcga
    19801 aatatattct tgagaaagtg aaaccgcagc gtattgctat cgttcacgac aaacagcaat
    19861 acggcgaagg tctggcacga gcggtgcagg acggcctgaa gaaaggcaat gctaacgtgg
    19921 tgttctttga tggtatcacc gccggggaaa aagatttctc tacgctggtg gcgcgtctga
    19981 agaaagagaa tatcgacttc gtttactacg gcggttacca cccggaaatg gggcaaatcc
    20041 tgcgtcaggc acgcgcggca gggctgaaaa ctcagtttat ggggccggaa ggtgtggcta
    20101 acgtttcgct gtctaacatt gcgggcgaat cagcggaagg gctgctggtg accaagccga
    20161 agaactacga tcaggttccg gcaaacaaac ccattgttga cgcgatcaaa gcgaaaaaac
    20221 aggacccaag cggcgcattc gtttggacca cctacgccgc gctgcaatct ttgcaggcgg
    20281 gcctgaatca gtctgacgat ccggctgaaa tcgccaaata cctgaaagcg aactccgtgg
    20341 ataccgtaat gggaccgctg acgtgggatg agaaaggcga tctgaaaggc tttgagttcg
    20401 gcgtatttga ctggcacgcc aacggcacgg cgacagatgc gaagtaatca ttaatcggca
    20461 actttgggtt gccgccaaat tgctaatatc gagtacgttg cttcatgccg gatgcggcgt
    20521 aaacgcctta tccggcctat aagatccaaa gaaatcagta aattgcaaca cacattgtag
    20581 gcctgataag cgtagcgcat caggcaattt ctcgttggtc atcacttttg aaatcaactt
    20641 gacgattaac gcttctccca gccgccctgt tgcgccgtaa atcccagtgc ctgcataaac
    20701 gctgtcatca caccgcgatc ttccacgcct gcatccgcca tccaccagca tgaaacgcca
    20761 ggattgttac gcaaaacctc ttccagcaga tattgcccca caccgcgacg tcgggtgact
    20821 tcccgcacac gcagggaatc cagtgctccc tcggtgccgc ttaaggttac ccgtacggca
    20881 gcgagcaggc gctcgttaaa acgcgcggcg tagatacggt ggttatcgtc aacctgtaaa
    20941 gaggaagggg aatactccgg ccagatcttt tgcaggtcaa tccggtcctg gtcgctaaag
    21001 ttttctaatc gaatgatggt cagcttcatg ggtaacccgt gtaaatcaca aaagtggaac
    21061 cagtgtagcg aaataattta atcggaggct ttctcttttt tatttctttt ggcaggtgat
    21121 taatttttta acagcaataa ttacaaagtt aaaacattag agaatgaaaa atacccagca
    21181 taatcccctg aatggtagtg aattattccg ccctttgtgc cgttatttta tgctgacaaa
    21241 ggcacttttt
//
LOCUS       DAZOZD010000054        21189 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOZD010000054 DAZOZD010000000
VERSION     DAZOZD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.054"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="ACHUPB_001617"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="ACHUPB_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR0758465.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="ACHUPB_001618"
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="ACHUPB_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFR0758466.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="ACHUPB_001619"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="ACHUPB_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR0758467.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="ACHUPB_001620"
     CDS             2861..3529
                     /locus_tag="ACHUPB_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR0758468.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="ACHUPB_001621"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="ACHUPB_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR0758469.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="ACHUPB_001622"
     CDS             4072..5904
                     /locus_tag="ACHUPB_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR0758470.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5894..6244
                     /locus_tag="ACHUPB_001623"
     CDS             5894..6244
                     /locus_tag="ACHUPB_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR0758471.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHUPB_001624"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHUPB_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR0758472.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="ACHUPB_001625"
     CDS             6563..6985
                     /locus_tag="ACHUPB_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR0758473.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="ACHUPB_001626"
     CDS             6988..7620
                     /locus_tag="ACHUPB_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR0758474.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="ACHUPB_001627"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="ACHUPB_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR0758475.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /gene="pduN"
                     /locus_tag="ACHUPB_001628"
     CDS             8112..8387
                     /gene="pduN"
                     /locus_tag="ACHUPB_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR0758476.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="ACHUPB_001629"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="ACHUPB_001629"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR0758477.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="ACHUPB_001630"
                     /pseudo
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="ACHUPB_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10799..11911
                     /locus_tag="ACHUPB_001631"
     CDS             10799..11911
                     /locus_tag="ACHUPB_001631"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR0758478.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            11908..13253
                     /locus_tag="ACHUPB_001632"
                     /pseudo
     CDS             11908..13253
                     /locus_tag="ACHUPB_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            13256..13810
                     /gene="pduT"
                     /locus_tag="ACHUPB_001633"
     CDS             13256..13810
                     /gene="pduT"
                     /locus_tag="ACHUPB_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR0758479.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13810..14160
                     /gene="pduU"
                     /locus_tag="ACHUPB_001634"
     CDS             13810..14160
                     /gene="pduU"
                     /locus_tag="ACHUPB_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR0758480.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14165..14608
                     /locus_tag="ACHUPB_001635"
     CDS             14165..14608
                     /locus_tag="ACHUPB_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0758481.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14709..15038)
                     /locus_tag="ACHUPB_001636"
     CDS             complement(14709..15038)
                     /locus_tag="ACHUPB_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0758482.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15210..16268)
                     /locus_tag="ACHUPB_001637"
     CDS             complement(15210..16268)
                     /locus_tag="ACHUPB_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0758483.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16466..16939)
                     /gene="sbmC"
                     /locus_tag="ACHUPB_001638"
     CDS             complement(16466..16939)
                     /gene="sbmC"
                     /locus_tag="ACHUPB_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR0758484.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17058..18224)
                     /gene="dacD"
                     /locus_tag="ACHUPB_001639"
     CDS             complement(17058..18224)
                     /gene="dacD"
                     /locus_tag="ACHUPB_001639"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR0758485.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18433..19860
                     /gene="sbcB"
                     /locus_tag="ACHUPB_001640"
     CDS             18433..19860
                     /gene="sbcB"
                     /locus_tag="ACHUPB_001640"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0758486.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19903..20130)
                     /gene="tsuB"
                     /locus_tag="ACHUPB_001641"
     CDS             complement(19903..20130)
                     /gene="tsuB"
                     /locus_tag="ACHUPB_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0758487.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20144..>21189)
                     /gene="tsuA"
                     /locus_tag="ACHUPB_001642"
     CDS             complement(20144..>21189)
                     /gene="tsuA"
                     /locus_tag="ACHUPB_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0758488.1"
                     /translation="LSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAI
                     SVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLI
                     GSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITL
                     WVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGR
                     MFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQT
                     TLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVR
                     PQRKARLATAAAN"
BASE COUNT         5450 a         5542 c         5264 g         4933 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcgggtgcc gcagctgcgc ataacgtcgg cgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccgaaaa ccactcatca aatacgagag acggctatgg cagaaaaaag
      481 ctgcacgcta acagaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aagcgctatc gttccattgg
      601 cattctgggt gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgc gatactaaag gcggtgccgg
      721 acacggctca ctgatcctct taggtggcaa cgatgtttcc gacgtgaaac gcggtattga
      781 agtcgccctg aaagaactcg atcggacctt tggcgatgtc tatgccaacg aagccggaca
      841 tcttgaactg caatataccg cccgcgccag ctatgcactg gaaaaagcgt ttggtgcacc
      901 actgggacgg gcttgcggcg ttattgtcgg cgctccggct gctgtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgttgtggca tacagctccc cagctcaaga
     1021 caccagctac agcaatgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cacccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttt attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccagccag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgccgaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcacgtcac
     1621 caacgtcaaa gataacccag tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccaatgg tgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcttctc gtggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttac ctcgaggctc gttgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggt tcggtaagct gcatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggcaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggt gggctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtgga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccttggcg caaggcagtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa cccagcgaca agtccgtttc
     2941 atttcgcgca cctgtctctg caactgtccc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acatcttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtaattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg cgaagcgcga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc actggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagggacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcat caatactgag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacagcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccct ggatatgcgc
     3781 attacccctg aaacattgcg tattcaggct gcgattgcca gagatgctgg tcgcgatcgt
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccagatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcaactaagc aagaacttat tgccattgct
     3961 gacgatcttg aacagcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgttcgt gagtagcgcc ctggaggaaa ccaccggaat caaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aactatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagtggg gctgggcgta gggttcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcgacga tgataaacgc ctccgtacgc gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcctggaaa ttcctttacc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg cagcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt attcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ctctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgcgttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga accaggcacc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg cggaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcagcgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga gtggggaaat cgttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tggcagaaga aatcaaaaag
     5461 tacccgttag caaaagtaga aagcctgttc cacttacgcc atgaagacgg tagcgttcag
     5521 ttcttcccta ctcccctatc cccccatgta tttgcccgcg tctgtgtagt gaaacccgac
     5581 gaactggtgc ctattcccgg tgatttgacg ctggaaaaag tacgcgctgt tcgtcgcagt
     5641 gcgaaagagc gtgtcttcgt aaccaatgcc ctgcgcgcgc tacgtcaggt aagccctgcc
     5701 ggaaatattc gcgacattcc gttcgtcgtt ctggtgggtg gttcttcgct ggatttcgaa
     5761 gtaccgcaat tggtcaccga cgcgctggca cattatcgac tggtcgccgg gcgggggaat
     5821 atccgtggta gcgaaggtcc gcgcaacgcg gtagctactg ggctactcat cgcctggcac
     5881 aaggagtcga ttcatggaaa gtaaagagcg tgcaccagcc atcgttgtca tggagatcgg
     5941 tgactgtatc actacatggg atgaggtgct tcttggcatt gaagaggaag gtattccctt
     6001 tcgcattcaa cacattccgt caggtgaagt tatcgatagc gcctggctgg ctgctcgtca
     6061 atctcccctg ttggttggca ttgcctgcga ccgggaagaa ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gacgtatcaa caagataacc atacccggcg
     6181 cagcatcggc actaacgctg cccgcctggt aaaaggaatt cccttccggg aattccactc
     6241 ataatccaca ggagaaacgc tgtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg cagctggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cggtgatggt ccgcggtgat gttggcgcag
     6421 taaaggccgc cgttgatgcg gggagcgctg cggcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcggcataac
     6541 cccatttgct aaggagcaca gcgtgaagca atcactggga ttacttgaag ccagtggtct
     6601 ggctctcgcc atcagttgcg cagacgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatcgcga cgggagccag tttcgccgga cagcgaaatg gccttgtcgc
     6781 acacaaagtg attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 cgtgacggtt gaaacaagca aaacaccagc aacaaccgtc acctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccttgcagga aacagtgagc
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatata
     7081 catctttcat cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aacctggcca atacgctgct gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagcca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gtcattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggcgtt
     7441 acgcatggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcgctg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgag
     7561 gccaacgccg caggcgctga tagccctgac gtttttgcca cactggtgtc agtgccatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaat tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcatctatcc agatacacga agcgttagcc tggggaatgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat cgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcagccgat ctaccttcat cataatcgac
     7981 ttttaagtta tgccgatatc gcacagttaa gctcgggaat actggtgtta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaacc tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccgggt cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc agtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttcaggc cctaacgagg
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga gctaaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacagcgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agcgaacagc cgtcagcgca
     8641 acagcgttac atcggtacgg aagaaattgc cgcgttggaa aacgcgatcg atagtgcaat
     8701 gaacgccgtc cccccagttc acagcttcat tctgcctggt cgatgtgaag ccgcaagccg
     8761 catgcatttc gcccgcacgg tggctcgacg ggctgaaaga cggctggttg aactgacaac
     8821 ggaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgcctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacacacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcggatg aactgcatat
     9061 tcccgttgtc atcagcattg ttgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaagc ttgctgatac cgtgcaaccc gacgcgccgc tttacgggct
     9241 cgaaagccat atgcaaggaa aagtcgtcac ttttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgcccaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgtgag caacttgtcc ccgctacacc acgaacgcaa
     9481 agaaacgcta ttttccagac tgtcgatgaa gcggtctgcg cagcacacta ggccttttta
     9541 cgctttcaac agtgcccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcactcgata acacgccagg aattgaagat
     9721 ttaaccacca cagcactaac cggtgatggt ggcatggttt tgtttgagta ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg tgccaaaaac
     9901 gtttcgttaa aactgattac cacaatcgaa gacatcgcgt ttcgctgtag tggcattcat
     9961 aacttgatcg ttaccgtatc tgaaccgacc tttgctgcca ccgaacagat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggc ggtccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaaccctc cgtgcatcgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtattg ctgaaaaaag cctgattgtc gtggcatcag tggcagatta tttaattcag
    10261 cagatgcaaa gttttggcgc acttcttctt aactatgagc aaactgagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcgg caggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtc
    10441 gacgccgatg atagttgggt tacttgtgag caactaatgc caatgctgcc cattgtgaag
    10501 gtcaaggatt ttgacagcgc tctaaccctg gcgctgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactctca gaacgtctca cgactcaacc ttgccgcgcg cgttatgcaa
    10621 acctcaatat ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccgact ggagagggta caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcaa ttcgctagag aaacggaaat
    10801 gaatactttt tcactgcaga cgcgtttata cagcggacca ggaagtctca aggtacttag
    10861 ccgtttctct catagacaca tctggattat ttgcgatgcc tttctggcac gctcaccact
    10921 tattgacacg ttgcatcagg cgctgcccga cagtaatcgt ctcagtattt ttagcgaaat
    10981 aacgcccgcc ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgta gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcaattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtatcggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatatcgcg atccttgatc catctttggt
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcctc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaaaaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatggcc tttagccagg ccggactcgg
    11521 tttaaaccat gcaattgctc atcaactcgg cggacagttt catctgccgc atggactggc
    11581 gaatgcgctc ctgctcacgg cagttatccg ctttaatgct ggcgatccgc gcacagccaa
    11641 acgctatgcc cggctggcga aaacgtgcca tttatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaat acaacctgta cgctgccgac
    11761 tcttgccaat gcgctgaaag aaaaaaaagc agaatggtca atacgcatac cagatatggt
    11821 tcaggcggcg ctggcggatg caacgttgcg taccaacccg cgtgcagctg atgcctccgc
    11881 gattgcagaa ctgctcaagg ggttgttatg aacgtcggat taatctcctg cgacgaccag
    11941 accatccgcg agcgagtaca agcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgctgac actttcctgg tgaatgcggc ggaatgcgaa
    12061 cccatgttga aagtagatca acagttaatg gcgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagaaaaa
    12181 tatcaacggg caataaacgc actcacacca ctgttaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttaccccgc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttagc gtcggtgtgg tggtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcacc
    12421 gttaatggtg cagtggcgaa gccaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtggatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaagtttgat cacatcgctc gacacaccag tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gaatgcagaa tgaccgcagc
    12661 gtacttgccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttaa tcggtcacga actggcaccc catttattag tacgtgcgcg gttaactacc
    12781 aacagctcgc cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct
    12841 gcgccagtgt cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc
    12901 aggaattacg cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg
    12961 caaattatcg actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt
    13021 atcaggatgc gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac
    13081 gccagcacat cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg
    13141 ggcaatgtgt aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg
    13201 atggcatggt cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc
    13261 gaaagcggta ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc
    13321 catgctgaaa agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt
    13381 tctgctgatg ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc
    13441 ccttgctggt gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct
    13501 ttctgcaatc agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac
    13561 ctggagtgtc gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat
    13621 tcttgttcgc gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg
    13681 cgatatttca gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg
    13741 tttgttggtc tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt
    13801 ggagggataa tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt
    13861 aagcaggtca cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg
    13921 ggtttaccag acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct
    13981 atcatcgcat gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt
    14041 tttaccggtg ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag
    14101 gttacccgta cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga
    14161 cgttatgcaa cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca
    14221 atgtctgcgt ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac
    14281 caccattgat acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt
    14341 cagtgcctgc gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc
    14401 tttttcgcca ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc
    14461 agatttagcc aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc
    14521 gcacaacgtg ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt
    14581 gctgcataca gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg
    14641 gtgatacaca gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa
    14701 aacaggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag
    14761 atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct
    14821 tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc
    14881 ttgatgtaat cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt
    14941 ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat
    15001 tccagtacgt cctgaaatga aggcttggta gtttccattt ttatacccct gataatgtga
    15061 gagtcggatt cgtttaagca accgctattc gttagggcca acattactca tagctgccgc
    15121 agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc
    15181 agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc
    15241 gatagcaact caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt
    15301 gtttccacta ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca
    15361 tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga
    15421 acaaagctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc
    15481 aataacacga aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata
    15541 caaaccagat tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt
    15601 tctttgctgg cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt
    15661 agatggcttt ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc
    15721 cgattatact cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt
    15781 ggccagatac cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc
    15841 cataacgccg tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca
    15901 atcaataatc cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc
    15961 gccgcgcacc agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga
    16021 cctaaaatcg aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta
    16081 ccccagaacg agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc
    16141 gggatagtaa acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta
    16201 ccatgcacta tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg
    16261 gcacgcacgc agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca
    16321 gaaggcgcgc ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg
    16381 agcgaaaaaa ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg
    16441 cgttaacaca ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt
    16501 cgatatccca gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg
    16561 gtaacatttc ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag
    16621 caaaatcatc accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg
    16681 tcagaatgac gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg
    16741 tgtcgcagcg taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc
    16801 actccttcgg cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct
    16861 ttaccgtctg ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct
    16921 gcgtaatctc gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc
    16981 aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt
    17041 ctgctgcaaa agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg
    17101 ccgccttccc cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg
    17161 tcgtaaagtt caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta
    17221 ccatcaaggg tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag
    17281 aactcttgtt ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt
    17341 ccgacctttt tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc
    17401 agtaattttc ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca
    17461 atgagacgac gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta
    17521 tgacccgttt tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt
    17581 tgctgggtga taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc
    17641 tcgccgtgga tgatagcgcg agaaaacaca gctaaatcat aagccgagct atgctggcca
    17701 ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca
    17761 tagttgttca tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca
    17821 acacaagcgt catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg
    17881 cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc
    17941 cacgcatcgc gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg
    18001 cgatccacga cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc
    18061 tcattacccg cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca
    18121 tgaatctctg gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag
    18181 ttaaaaacga acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga
    18241 gtttcaaata gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag
    18301 aaaatgtgac taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt
    18361 gtggcgctaa agctgattag cacggtgata tttgatactc tggcagacag cagaaataac
    18421 ggatttaacc taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac
    18481 gaaacctttg gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc
    18541 gataacgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac
    18601 tatttacccc agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg
    18661 aaaggagaaa acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag
    18721 acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt
    18781 tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat
    18841 ttgctggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa
    18901 aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa
    18961 catagcaacg cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg
    19021 gtaaaaacgc gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa
    19081 ctgatggcgt tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt
    19141 ggggcatggc gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc
    19201 aatgccgtaa ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc
    19261 gacacattgc gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt
    19321 ccggttaagc tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc
    19381 ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt
    19441 ctgcgtgaaa atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg
    19501 tttacgcctt cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat
    19561 cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc
    19621 acttttgtcg ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg
    19681 gggacgctgg attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg
    19741 cccgagtttt tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat
    19801 aacaaagaga aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa
    19861 tttgaagcct ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt
    19921 gatactccac gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc
    19981 cgcccactgt gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc
    20041 gccgctggcc atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg
    20101 cgtcaccaca tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag
    20161 ttgccagtcg cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc
    20221 cgagaatcat aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca
    20281 gtccattacc gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac
    20341 ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg
    20401 ctttggctgc aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa
    20461 tgtatttcac gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc
    20521 ctaacccaaa catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac
    20581 cgatgagtac agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc
    20641 cagttcggcg cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca
    20701 ccacccagag cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag
    20761 tctcagcaat agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg
    20821 catgtggaga acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc
    20881 cgatcaatcc ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa
    20941 ttcccagccc gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt
    21001 cgtaagtcag taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg
    21061 caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac
    21121 ctgtcaggca aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc
    21181 cgcttaata
//
LOCUS       DAZOZD010000055        21160 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOZD010000055 DAZOZD010000000
VERSION     DAZOZD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21160
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.055"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUPB_001643"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUPB_001643"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0758489.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUPB_001644"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUPB_001644"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0758490.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUPB_001645"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUPB_001645"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0758491.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUPB_001646"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUPB_001646"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR0758492.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUPB_001647"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUPB_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR0758493.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUPB_001648"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUPB_001648"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0758494.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUPB_001649"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUPB_001649"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0758495.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHUPB_001650"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHUPB_001650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0758496.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUPB_001651"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUPB_001651"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0758497.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHUPB_001652"
     CDS             complement(12799..14139)
                     /locus_tag="ACHUPB_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0758498.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUPB_001653"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUPB_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0758499.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHUPB_001654"
     CDS             complement(15928..16377)
                     /locus_tag="ACHUPB_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0758500.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUPB_001655"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUPB_001655"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0758501.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHUPB_001656"
     CDS             complement(17177..17506)
                     /locus_tag="ACHUPB_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0758502.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUPB_001657"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUPB_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0758503.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHUPB_001658"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHUPB_001658"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0758504.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUPB_001659"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUPB_001659"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0758505.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5160 a         5679 c         5053 g         5268 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca
//
LOCUS       DAZOZD010000056        21003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOZD010000056 DAZOZD010000000
VERSION     DAZOZD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.056"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUPB_001660"
     CDS             complement(<1..170)
                     /locus_tag="ACHUPB_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR0758506.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUPB_001661"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUPB_001661"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0758507.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHUPB_001662"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHUPB_001662"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0758508.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUPB_001663"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUPB_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0758509.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUPB_001664"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUPB_001664"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0758510.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUPB_001665"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUPB_001665"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0758511.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHUPB_001666"
     CDS             4288..5151
                     /locus_tag="ACHUPB_001666"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0758512.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUPB_001667"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUPB_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0758513.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHUPB_001668"
     CDS             6489..7106
                     /locus_tag="ACHUPB_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0758514.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUPB_001669"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUPB_001669"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0758515.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUPB_001670"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUPB_001670"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0758516.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUPB_001671"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUPB_001671"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0758517.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUPB_001672"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUPB_001672"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0758518.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUPB_001673"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUPB_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0758519.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHUPB_001674"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHUPB_001674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0758520.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUPB_001675"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUPB_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0758521.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="ACHUPB_001676"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="ACHUPB_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0758522.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="ACHUPB_001677"
     CDS             18072..19781
                     /locus_tag="ACHUPB_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0758523.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHUPB_001678"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHUPB_001678"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0758524.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5713 c         5385 g         4822 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccacg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAZOZD010000057        20711 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOZD010000057 DAZOZD010000000
VERSION     DAZOZD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.057"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUPB_001679"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUPB_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0758525.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUPB_001680"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUPB_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0758526.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUPB_001681"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUPB_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0758527.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUPB_001682"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUPB_001682"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0758528.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUPB_001683"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUPB_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0758529.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUPB_001684"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUPB_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0758530.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUPB_001685"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUPB_001685"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0758531.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUPB_001686"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUPB_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0758532.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUPB_001687"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUPB_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0758533.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUPB_001688"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUPB_001688"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0758534.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUPB_001689"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUPB_001689"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0758535.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="ACHUPB_001690"
     CDS             complement(14275..16539)
                     /locus_tag="ACHUPB_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFR0758536.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUPB_001691"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHUPB_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0758537.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUPB_001692"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHUPB_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0758538.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUPB_001693"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHUPB_001693"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0758539.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUPB_001694"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHUPB_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0758540.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAZOZD010000058        20697 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOZD010000058 DAZOZD010000000
VERSION     DAZOZD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.058"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..696
                     /locus_tag="ACHUPB_001695"
     CDS             <1..696
                     /locus_tag="ACHUPB_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0758541.1"
                     /translation="EQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSSWGIN
                     NRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFVAQTA
                     MCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMHYFSA
                     YSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQ
                     PECPVTLKDVSDL"
     gene            693..2147
                     /gene="djlC"
                     /locus_tag="ACHUPB_001696"
     CDS             693..2147
                     /gene="djlC"
                     /locus_tag="ACHUPB_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0758542.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(2207..3877)
                     /gene="hscC"
                     /locus_tag="ACHUPB_001697"
     CDS             complement(2207..3877)
                     /gene="hscC"
                     /locus_tag="ACHUPB_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR0758543.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(3969..4904)
                     /gene="rihA"
                     /locus_tag="ACHUPB_001698"
     CDS             complement(3969..4904)
                     /gene="rihA"
                     /locus_tag="ACHUPB_001698"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0758544.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5022..5747)
                     /gene="gltL"
                     /locus_tag="ACHUPB_001699"
     CDS             complement(5022..5747)
                     /gene="gltL"
                     /locus_tag="ACHUPB_001699"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0758545.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5747..6421)
                     /gene="gltK"
                     /locus_tag="ACHUPB_001700"
     CDS             complement(5747..6421)
                     /gene="gltK"
                     /locus_tag="ACHUPB_001700"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0758546.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6421..7161)
                     /gene="gltJ"
                     /locus_tag="ACHUPB_001701"
     CDS             complement(6421..7161)
                     /gene="gltJ"
                     /locus_tag="ACHUPB_001701"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0758547.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7331..8239)
                     /gene="gltI"
                     /locus_tag="ACHUPB_001702"
     CDS             complement(7331..8239)
                     /gene="gltI"
                     /locus_tag="ACHUPB_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0758548.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8694..10571
                     /locus_tag="ACHUPB_001703"
     CDS             8694..10571
                     /locus_tag="ACHUPB_001703"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR0758549.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10649..12187)
                     /gene="lnt"
                     /locus_tag="ACHUPB_001704"
     CDS             complement(10649..12187)
                     /gene="lnt"
                     /locus_tag="ACHUPB_001704"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0758550.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12212..13090)
                     /gene="corC"
                     /locus_tag="ACHUPB_001705"
     CDS             complement(12212..13090)
                     /gene="corC"
                     /locus_tag="ACHUPB_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0758551.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13180..13647)
                     /gene="ybeY"
                     /locus_tag="ACHUPB_001706"
     CDS             complement(13180..13647)
                     /gene="ybeY"
                     /locus_tag="ACHUPB_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0758552.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13644..14684)
                     /locus_tag="ACHUPB_001707"
     CDS             complement(13644..14684)
                     /locus_tag="ACHUPB_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0758553.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14837..16261)
                     /gene="miaB"
                     /locus_tag="ACHUPB_001708"
     CDS             complement(14837..16261)
                     /gene="miaB"
                     /locus_tag="ACHUPB_001708"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0758554.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16407..17582
                     /gene="ubiF"
                     /locus_tag="ACHUPB_001709"
     CDS             16407..17582
                     /gene="ubiF"
                     /locus_tag="ACHUPB_001709"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0758555.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17736..17810)
                     /locus_tag="ACHUPB_001710"
     tRNA            complement(17736..17810)
                     /locus_tag="ACHUPB_001710"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17776..17778),aa:Gln,seq:ctg)
     gene            complement(17847..17921)
                     /locus_tag="ACHUPB_001711"
     tRNA            complement(17847..17921)
                     /locus_tag="ACHUPB_001711"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17887..17889),aa:Gln,seq:ctg)
     gene            complement(17970..18046)
                     /locus_tag="ACHUPB_001712"
     tRNA            complement(17970..18046)
                     /locus_tag="ACHUPB_001712"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18010..18012),aa:Met,seq:cat)
     gene            complement(18062..18136)
                     /locus_tag="ACHUPB_001713"
     tRNA            complement(18062..18136)
                     /locus_tag="ACHUPB_001713"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18102..18104),aa:Gln,seq:ttg)
     gene            complement(18171..18245)
                     /locus_tag="ACHUPB_001714"
     tRNA            complement(18171..18245)
                     /locus_tag="ACHUPB_001714"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18211..18213),aa:Gln,seq:ttg)
     gene            complement(18269..18353)
                     /locus_tag="ACHUPB_001715"
     tRNA            complement(18269..18353)
                     /locus_tag="ACHUPB_001715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18317..18319),aa:Leu,seq:tag)
     gene            complement(18362..18438)
                     /locus_tag="ACHUPB_001716"
     tRNA            complement(18362..18438)
                     /locus_tag="ACHUPB_001716"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18402..18404),aa:Met,seq:cat)
     gene            complement(18818..20482)
                     /gene="asnB"
                     /locus_tag="ACHUPB_001717"
     CDS             complement(18818..20482)
                     /gene="asnB"
                     /locus_tag="ACHUPB_001717"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0758556.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20436..>20697)
                     /locus_tag="ACHUPB_001718"
     CDS             complement(20436..>20697)
                     /locus_tag="ACHUPB_001718"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758557.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPDPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         5026 a         5203 c         5368 g         5100 t
ORIGIN      
        1 gaacaatatc ttctttttgc gctttctgcg cccatggaaa tacttaatca gagttgcaaa
       61 cccgcgcatg actcgccaaa aatgtatact gggataaaag aattcgatct ctcatcatcc
      121 tggggaataa ataatcgtga tgatttaatt cagactattt atcagatgac tgatgatggt
      181 catgccaatg atttagctgg cttatatctg acatggcaac gctcttcccc tgaagagagg
      241 aaagcattaa ttgctggtgg ctcggagaga gggttgattt atacccaatt cgttgcccaa
      301 accgctatgt gctgtggaga agggggaatc aaagcctggg attatgttcg aatggggttt
      361 ctgtcacggg tcggtgtgct caacaactgg ctgacggaag aagagagttt atggctgcaa
      421 tcgcgcgtct atgcacgagc tcaccattac tatcacagct ggatgcacta tttttctgcc
      481 tattcactag ggcgtctcta ctggcaatct tctcagtgcg aagacaacgc ttcgctacgc
      541 gaggcgctaa ccctgtacaa atacgatagc gccgggagtc ggatgttcga agagctggca
      601 gcgggaagcg accgtttcta tgccacgctt ccctggcaac cattaactgt ccagcctgaa
      661 tgcccggtaa cgcttaagga tgtgagcgac ctatgaaaac atgttggcaa atcctcgaaa
      721 ttgaaagcac gacgcaaata gacattatcc gccaggctta tcttgctcgc ttaccgttgt
      781 gtcatcccga aaccgatccg caggggttta aagcattacg ccaggcctat gaagaggcgc
      841 tgcgactggc ggtaaatcct gtcgaggaag ccgatgatga agaaaaagat gccaccgctg
      901 aacatgaaat actacgtgca ttcaggacat tactggattc agaaagtgat cgttttcagc
      961 cctccgcctg gcagaaattt attcagcaat taaatacctg gaacatggag gatgtcgatc
     1021 aattacgctg gccgctgtgt gcaatcgcta tagaagcgcg atatctttca ttaaattgtg
     1081 cttctttgtt ggcagagcgt ttgaactggc attcgtttaa tgacagcgaa ggaatggatg
     1141 atgaggaaga aagggaggct tttcttgagg ccattcaggc tggtgattgt ttcgatttcc
     1201 ttagccttct ggaatatccc gtcgcgttac agaaccagac tgttgaatat tacttcgcgc
     1261 tggaacgttg ctgccgttac catcctgact atgtcactgc gtttttggcg atggaaggac
     1321 cgtggttcat tcctgatgat gcaaagttac atcgcaaact gttgcgctgg tacagctcgg
     1381 tgcaaacagg tatggcggaa ctcattcctg tagccaaaca gtggcaagcg gaagaaccag
     1441 aaagcgaaga tgcccggtat taccagtgtg cacaacgttt gtactgcggc gagggggaaa
     1501 gcctgcttgc cgatctctgc gcgtactggg aaagttaccc atctacacaa gctgataatt
     1561 tgttgttgca gtggagcaag cgtcattgcc cggattattt cgcgttatta gtgatggtta
     1621 tcgaagcgcg aagcatggta gatgcgcaag gtaaaccgtt gaaatatgtt cctggtgaga
     1681 gcgcccggac gcggctgtta tgggcggaga ttttacatag cggaaaatta tcgccgttag
     1741 gtcaatcgtt tattgagtcg ttattcttca agcgcaaagc atgggcgtgg tggaaatcga
     1801 gagtcggtag cgagacagag caagattcac cgttactgga tttgtatcgg gtagcggaac
     1861 aggttgtgct ggaagcgttc ccgaaacaag agatgctggc ccgtcttaat acaaggctgg
     1921 aaggtggaga tactcatcca ttagaggcca ttatcaccca gatgctgttg acgaaagtga
     1981 aactcgagcc ggaggatgaa gatgtcgatg agccaacacc tgaaaaccat gaagaaaaaa
     2041 atgatgagga tgaaaaacca cagagcatta ccagtattat caaaatcagc ttaacggtgc
     2101 tgatgatagg ttatgcgctc ggaaaaatcg cgatgttgtt tagctgagga tgtgtttttt
     2161 gcgccgatgc cggatgcagc gtttatgccg catccggcag ccgtgtttaa gggatctcaa
     2221 tagccaaata atcgctcgcc cgtcgacgaa cttcatcgac gcgggccata tcgtttgact
     2281 gcaacgccgc atcaaaatcg gtgatcaccc ggccaatctc ttctcgctcg tcacccagcg
     2341 cccgcgccca caactcttcc agtttggcct taaaggtgcg gttgattagc atatcgcgcg
     2401 gataaatttt caatgcggat aaccgcgtcc gactctcttc aatctgctgc gcgctcaatg
     2461 ttaccgggct atgattaatg actctggact taacgctgcc atcttccaga agcacgtcaa
     2521 cttcaagcaa gccgttgata tcataactaa agcgaatatc aatcgactga taagccccgg
     2581 ttttcttcaa cggcacatcg aaggattcaa ccagaatgtt atttttaact ttgtggtttt
     2641 ctccctgata gacgttaacc gtaatagaat cctgttccgg gtgcatggtc gagtaagttt
     2701 ctacacgcga cacgggtact gtggtgtttc gttcaataat tggcgagaaa atgccggaaa
     2761 cgccctggcg gttaacttca acgcccaacg agtaagggca aatatcagtg aggatcacct
     2821 cttcaatatc ttcactgcgt aagcggcagg cggcctggat tgctgcgccc agcgcgacaa
     2881 tggtgctcgg gtcgtaactt tgatacggta atttgccaaa cagacgcacg gcgattcgct
     2941 gcaccagcgg catttgtgac gcgccgccaa ccagcaccag actgtcgatt tgactcggct
     3001 tcagacgtgc atcgcgcaac gcctgttcaa tcggcactcg caagcgattg agcagcggca
     3061 accacaaatc ttccagttcg ttctcgtaaa attcgcactc ccgcgtttca tcctggtact
     3121 gccagcgaat gtgcaatggc gattgattgc tacattttgc cgcttccaca caggcgtaca
     3181 aggctgccag ttcactctcg ttaagcgtgg tcctggcgac atccgcgcgc tttaaaacgt
     3241 catcgaccaa catatgggta aaatcttcgc caccgaggaa gttatcgcca gcggaggcgt
     3301 gaacttcaat caccggcgtg gcgtattcga gcaccgtgac gtcaaacgtg ccgccaccga
     3361 gatcaaaaac cagcgagcgg gtattttgtt gcgtatgcag gccatatgcc atcgcagctg
     3421 ctgtgggttc attaattaag cgtaccgcat ttaacccggc taactccgct gctaaacggg
     3481 tatgcttgcg ttgttcatcg ctgaaataag ccggaacgga gatcaccaca tctttaatcg
     3541 gacgttgcag aaattcttcg gcatcttctt ttaatgagcg taataccaaa gaggacagtt
     3601 ctggcgcgtt aaatgtgtcg ctgcctaacc gccagttggt attactgccc attgcacgtt
     3661 taaataacgc tgccgtttta tccggatgcg aagtacgccg tgatacagcc ggttttccga
     3721 ctaaaatatg attattttca tccatgctaa ttatggatgg tgttaaatat tcaccgaact
     3781 tatttggaat taattgcgcg gcaccgtctt tccagacggc aattaaacta ttggtagtgc
     3841 cgagatcgat accaatggcg agttctgcat tatccattgc atttatatct tgaaagaaaa
     3901 agagataaac agataaacag attaagacca cccaggtaac gcgtggtgat cttaatcaat
     3961 gacgtgtgtt aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga
     4021 tcaacatcga ccattacagt ggcattcggt ttattgccag tcagataata ataatcaaca
     4081 accgtcatac cctggctata tttcccctgt gtttccacgc caacccagcg ctcaacagtg
     4141 gtaaacaact cgggtttcaa cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg
     4201 acaaagcccc atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca
     4261 atggttgaaa cagggttgcc aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgt
     4321 gctttgtgag taacatccag accggccatc accactggga tccctgactg gaagacaatt
     4381 tctgccgctt ccgggtcaac gtaaatatta aattcagccg caggcgtcca gttaccaagc
     4441 cccattgcgc cgcccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta
     4501 ttgagcagca aggcaacgtt agtttgcggt ccggtagaca caatcgtgac aggttccgca
     4561 ctttcgcgca gcgttttcgc catcagctct accgccgtac agttttgcgg tgcgaacgtc
     4621 ggttccggta atgccgggcc atccagaccg ctttcgccgt gcacattgtc cgcgataatc
     4681 aactcacgca ttaacggttt taccgcgccg cctgctaccg gaatatcggt gcgattaagc
     4741 aaggtcagca tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa
     4801 gacgtaattg ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg
     4861 tcgtcatgac ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt
     4921 gtgcttcttt gcgacaaggg taacgccagg atgtaacaga tacgaggggc gaaacgataa
     4981 agcgtgagat ggcgcgcact tgggtatgcg cgccagagtg attaatgcag gattttcgcg
     5041 aggaagtctt tcgcgcggtc cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct
     5101 tcgacaattt taccctcgtc cataaagatc acccgattcg ccactttgcg ggcaaagccc
     5161 atttcgtggg tcaccaccat catggtcatt ccttcgttcg ccagttccac catcacgtcc
     5221 agcacttcgt tgatcatctc cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca
     5281 ataggatcca tacacaatgc gcgagcgatt gccacacgct gctgctgacc gccggaaagc
     5341 tgcgccggaa atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct
     5401 ttttcacgag cgggcgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt
     5461 tcgataatcg acagatgagg gaacagctcg aaatgctgga ataccatccc gacgcgggaa
     5521 cgcagctttg ccagatcggt tttcttgtca ttaaccacga taccatcgac ggtgatttca
     5581 ccttgctgca ctggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca
     5641 gacgggccgc aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc
     5701 acctgaaagt gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc
     5761 ttttcaagta gctgaccaac aacgacgcgc taagactaat aacgaaataa acaaatccgg
     5821 caaacaggat catctcaacc tgcgtaccat cacgctcacc aatggttgag gcggtacgga
     5881 agaaatcggc cagagataac acatacacca gtgaggtatc ctggaacagt acgatgccct
     5941 gagtgagcag aagcggcacc atcgcgcgga acgcctgcgg cagaataatc agtttcatcg
     6001 actgccagtg agtcattccc aacgccagcg cggcgctcga ctgaccacga gaaatacttt
     6061 gaataccagc acggataatc tctgaatagt aggccgcttc aaacatcgaa aacgccacca
     6121 tcgccgaaat taaacggata tcattttttg gtgataatcc cagcacgttt tgcagaaaac
     6181 ctggcacgat caggtaaaac cacagcaaaa ccataactaa aggaatcgag cggaatacgt
     6241 taacgtaggc tttggcaaac cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca
     6301 gcatggtgcc ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agcgtgatca
     6361 ccagcccgtc gagcagatat ggcagggaag ggacaatgga actccagtca aactcgtaca
     6421 ttatttgccc cccatgttgc caggcaggcg aactttacgt tcaaccagcg tcattaccag
     6481 catgataacc gcgttaatca acacatacgc cagcgtaata gcggtaaacg actcccaggc
     6541 gtgggctgag taatcaagca atttacccgc ctgcgccgcc atatccacca gaccaatagt
     6601 cgaggcgatg gcggagtttt tcaccaggtt catcatctcg gaagtcatcg gcgggacgat
     6661 aacgcgataa gcattaggca gcagtacgta tcgataagcc tgcggtagcg tcaggcccat
     6721 cgccagcgcg gcattttttt gcccacgcgg caacgactga atggcggcgc gtacctgttc
     6781 gcaaacacgg gcggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat
     6841 attgggatcc agctcggcct taaaccacat gccgattttc tccggcaaca gctccgggat
     6901 caccagatac caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata
     6961 caacgtaccc agaccggaga gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga
     7021 accgacgagg aaagcgataa tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc
     7081 actccagatc caaccaagat aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc
     7141 ccagttccag tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg
     7201 ctaccctcga agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca
     7261 tcaggcgtat agtctgtccg tgctacgtaa caatcgagag ggctggaatt tccgcccctg
     7321 gttcttgtaa ttagttcagt gccttgtcat tcggttcttt gaacagtgct ttcatttcgt
     7381 ctgacagttc gaaattcatg ttcaggtttt tcggcggaat gggatttttg aaccatttat
     7441 caaaccattt ttccgcttca ccggaggtct gcacctgcgc ggtggtgtca tccatcagct
     7501 ttttgaactg cggatcgtct ttacgcaaca tgcaaccgta ggcttcctga gactgcggct
     7561 tgccgacgat ttcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcaggg
     7621 catcatccat cataaaggca acggcacgac cgctttccag ggtgcggaaa gagtcactgt
     7681 gatctttggc gctgatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca
     7741 aaacttcaga ggtagtgccg gaagtgacga ctacggcttt gtctttcagg tcggcaaaat
     7801 ctttgatatc gccgcccttt ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag
     7861 agaaagccgc ctgtttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcga
     7921 aagtgccgtt ttgcagcagt ggaatacggt tttgtgaggt aatcggaatc agttttacct
     7981 gcaagtccgg tttgttgagt ttctttttca ctgcttcaac aatggcgttg gagtaatcct
     8041 gcgagtaacc caccactttt tgctgattgt cgtaataaga gaaaggtact gaagattcac
     8101 ggtgaccgac gacaatcaca ccgtttttgg cgattttgtc cagcgtactg cctgctgccg
     8161 gggcggcgtc atctgcctgt gccagtcctg cggaaagcgc cagggcgagg attgctgtgg
     8221 caggtttacg taattgcata tccaactcct ttatcttctg cgttaagaac gcatggatac
     8281 ccgttgtgag tgtttgtgtt gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt
     8341 aggtaacatt tagtttggct aaatgtaaag atattgctgt tttattgttt gtttttgcga
     8401 gatgcgccgc accattccga agcaaaattc ttaaaccgca cctttttggt gctaccgctg
     8461 atttgtggtg gtgcaacctg gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa
     8521 agcaacttac atgccaaaaa atacttaggg gcgaattaat tacgattgta attttaggct
     8581 gaaaaagtca tatttcggac agaattatct tttcggcatg ggacgtttgc cgggatgata
     8641 actgcacacg ggaaaaatgc tttttacctg tcttttcaag gaagaaacac cttatgtctg
     8701 catcttcggt taagccgtta aatgttcaat tacccgcaat aacccttatc ctttttgcac
     8761 tctgtattgg gatattttgt tatctcgcac aatggatgag ttatgaagag gtcgatcaat
     8821 ccgcacttat ccatctcggc gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc
     8881 gcttattgag cagtatcttt ctgcacagta gtgtttctca tctgctgatg aatatgtttg
     8941 cattcctggt cgtgggaggc gtggcggaac agattctggg gaaatggcga ctcctgatta
     9001 cctggttatt ctccggcgtc tttggtgggc tcatcagcgc ctgttatgcg ttacgcgaaa
     9061 gtgagcagat agtcatcagc gttggggcat ccggggcgat tctgggaata gctggtgcag
     9121 cgatagcaac acagtttgct tcaggtacgg gcacatacca taaaaaccag cggcgagtat
     9181 ttcctctgtt gggtatggtg gcgctgacac tgttgtacgg tgcccggcaa acaggaatag
     9241 ataacgcttg ccacattggc ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc
     9301 gtttatctgg gcaaaaccga ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc
     9361 ttctgaccgg ggctatctgg cttgcgcagc agcagatgga tgagtcagtt ttacaggtca
     9421 ggcaaagcct gcgtgaagcg ttttatccac aggagattga acaagagcga cggcaaaaaa
     9481 agcagcagtt agcggaggaa cgcaacgccc tcagggaaac attatccgct ccggtaagtc
     9541 gtgaacaggc cagtggtgat ttgctcgctg agattgccga tatccatgat atggcgatca
     9601 gtcgggatgg taatatgttg tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg
     9661 acctcggaca aaagaaaatc ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag
     9721 tcaaacattg tggtggctgt aaagatcagg gcgtcagatc gctggcatta agcccggatg
     9781 aaaagttgat ttatgcgact tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga
     9841 caggggagat tattcagtcg attaccaccg gtgcacatcc tgacagcctt atcctctcgc
     9901 gtgatggcac aaaagcctgg gtgatgaatc gcaccagtaa tagtgtgcca gcgattgatc
     9961 tggtgactta tcagcatgtg gcggatatcc cgctggagaa atacgacggg acggggacga
    10021 gcggtaaacc aggcgcctgg gttatggcac tttccccgga tgaaagaaca ttactggttc
    10081 cgggagcagg cagaggtaac atcgtgcgga tcaataccat cacgcatcag aaagaagact
    10141 ttcccgcagg tgatgcgcgt ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg
    10201 aggttatttt tgccgatagt caggggattt cacgtataag cgtaggggat caacaagcca
    10261 gcattatgac gcaatggtgt agcaggagcg tttattccgt tgagggtatt agcccggacg
    10321 gtcaatattt agcgttggtg tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta
    10381 atgccgggca gattattggc gtttatcctg ccagctacgt taatcacctt cgtttttcag
    10441 cggatggtag aaaaatattt gtcatggcga agaacgggtt gatccaaatg gacaggacgc
    10501 gctcgcttga tccgcaggca attattcgtc atccccaata tggcaatgtg gcttgtatcc
    10561 ctgaaccgta agaaaaaggg gcatgataac catgccccaa agctcaatga gaaagtacgt
    10621 tttgctcatg caggatacat caggcacatc aacgtttacg tgctcgcaga ctcatcaaca
    10681 cagcggcaaa accaaacagc gccgtcagca cccacagtgg ccagttgccg gtacgtgcat
    10741 acggtgtgag tccggtggtc ggtgtcacat tagtggtcaa cacctcgcgg gtgaactgcg
    10801 ggatcatcgc ctgaacctcc ccctgcgggc caatcaccgc cgtaatgccg ttgttggtgc
    10861 tgcgcaacag tgggcgcgcc agctccagcg cacgcattcg cgccatctgg aagtgctgcc
    10921 atggaccaat agacttacca aaccacgcat cgttggagat agtcagcaga tagtcggtat
    10981 ccgggcggaa gttatcgcgc acttgctcgc cgagaatgat ctcgtagcaa atagccgcag
    11041 taagctcaat accatttacc gacagcggcg gctggatata tggcccacgg ctgaacgacg
    11101 acatcggcag atcaaagaac ggtgctaacg gacgcagaat cgactccagc gggacaaact
    11161 cgccaaacgg cactagatgg tttttgttat agcgatcggc tgattcgtag ctgtatggtg
    11221 cacctttacc cagtgtgatg atggtgttgt aggtatcgta gcggttctgc ttattgagac
    11281 gcgcgtcgac aatcccggtt accagcgagc tacctttatc gcgcaactca ccgtccagtg
    11341 ctttgaggaa cggttgctga ttaatttcca gatcggttat cgccgactcc ggccagataa
    11401 tcaacgatga tttgcccatc agcggagccg ttgcgttgta gtaaatcttc agcgtattaa
    11461 gaagctgatc tccatcccat ttcagtgatt gcggaatatc gccctgaacc atcgaaacct
    11521 gaatggtttt ctccggttgc ggggtaaacc attggatgta acgcagcggg aagggtaggg
    11581 caaacagcac gacggccacc accagcggac gccagttgcg tttgaccaac gccagtgcca
    11641 gcaggccact aaccatcatc agcaggaagt taatggcttc cacgcccatt atcggtgcca
    11701 gcccttttaa cgggccatca atctggctat agccgaactg taaccacggg aagcctgtca
    11761 gtacccaacc gcgcagaaac tcggtcactt gccagagagc aggggcggca atcgccacgc
    11821 gcagccaggt ggttttcggc cacagacgcg acagcacgcc agcaaacagt ccggtataca
    11881 gcgacaaata cgccgccagc agcaccacca ggaagatgtt aaccgggcca ggcattccgc
    11941 caaaggtcgc gatgctgaca tagacccagt taataccgct gccaaagagg ccaaatcccc
    12001 agcaaaagcc aatagcggca gactggagtg gacggcggtt aaaggtcaac gcctgaagcc
    12061 ccatcaacga aataatcgcc gcaggccaga cgtcgtaagg agagaaggcc agcgttccgc
    12121 aggcaccgaa taataacgcc agcagcaggc gaatgcgctg gcgttcaatt aatgaggcaa
    12181 aagccatgta gttatctatc cagtttcggt tttattcatc cagcttcggc tggggtgagt
    12241 catccgggat tttgacatga acctgaataa tacgccgact gtcggccatc gccactttga
    12301 actggtaacc gtcgatgtcg atagtttcgc cacgcgccgg aagatgccca aatgcctgca
    12361 tcaccagacc accgatagtg tcaacctctt catcgctaaa gtgggtgccg aacgcttcgt
    12421 tgaagtcttc aatggaagcc agtgcacgca cggtccaggt atgacgactc agctgacgga
    12481 agtcgatatc atcttcttcg tcatactcgt cttcaatctc accaacaatc agttccagga
    12541 tgtcttcaat ggtcaccaga ccggaaaccc caccgaattc gtcaatgacg atcgccatgt
    12601 ggtaacgctg agagcgaaac tctttcagca tccggtctac gcgcttactt tcaggaacga
    12661 caaccgcctg acgtaacact ttgtccatgc tgaaggcttc agcatcgctg cgcataaacg
    12721 gcagcaagtc tttcgccatc agaatccctt caatgtgatc tttgtcttcg ctaatcaccg
    12781 ggaaacgtga gtgggcggac tcgatgatga catcaaggca ttcgtccagc gtctggttgc
    12841 gtttcagggt aatcatctgg gagcggggga tcatgatgtc gcgaacgcgt tggtctgcga
    12901 tgtccatcac cccttcgagc atatcgcgcg tatcttcgtc gataaggtcg ttctgcccgg
    12961 aatcacggat cagcgccagc agttcgtcac ggtttttcgg ttcaccgtgg aaaagttggc
    13021 tgagtaacag ggagaaaaat cccttcttgt tgcttatcgt gtcactactg tgtgaattgt
    13081 cgtcgctcat ggcgtcgtat gggttctcat gttagttaat caaaacgtcg tcgttaatca
    13141 ccaacggcgg ggacgtctgc cagtcaaatg cctggcaaat tattctttct cggcaatgta
    13201 cggatcctca tagcccagag caagcataat ctctgtttcg agggcttcca tttcttctgc
    13261 ttcgtcatct tcgatgtgat cgtaacctaa caaatgcaga ctgccgtgca ccaccatatg
    13321 cgcccagtgc gcctccagtg gtttgccttg ctcctgagct tccttctcaa ccacctgacg
    13381 gcagataacc agatcgccca gtagcgacat ttccatgcca ggcggcactt caaacgggaa
    13441 ggagagcacg ttggtcggct tatccttacc gcgataggtc agattcagac tgtggctttc
    13501 ggcggtatcg accacgcgaa tagtcacttc cgattcttcc tgaaactgcg ggatcaccgc
    13561 attcagccat gtttgaaact ggctctcttc cggtaacccg gaattatctt cacatgccag
    13621 ttgtaaatcg aggatcacct gactcatttt tgttcctgtt cttcgcgctt gcgttctgcc
    13681 gccagcgccg cttttcgttt ttgttcggct tcttcccagg cttcataggc gttaacgata
    13741 cgcgccacca cagggtgacg aaccacgtct tcgctgtgga agaagttaaa gctgatctct
    13801 tcgacatcgg ccagcacttc gatggcgtga cgtaagcctg atttagtatt acgcggcagg
    13861 tcgatctgtg tgacgtcgcc ggtgataacc gcttttgagt taaaaccgat acgggtcagg
    13921 aacatcttca tctgttcgat ggtggtgttc tggctctcat cgagaatgat aaacgcgtcg
    13981 ttcagcgtac gaccacgcat ataggccagc ggcgcgactt caataacgtt gcgctcaatc
    14041 agtttctcaa ctttctcaaa gcccagcatt tcaaacagcg cgtcgtacag tgggcgcaga
    14101 tacgggtcta ctttctggct taaatcgcca ggcaggaagc ccagtttctc accggcttcg
    14161 accgccggac gagtcagcag aatacggcga atctcctgac gctccagggc atcaactgcc
    14221 gcagccactg ccaggtaggt tttacccgta cccgccgggc caacgccgaa ggtaatgtca
    14281 tggtcgagaa tattggcgat gtactgcgcc tggtttggcg tgcgcggctt aattacgccg
    14341 cgtttggttt tgatattgac cgctttgccg tactccggca cgctctccgc actttgctcc
    14401 agtactcgcg cttctttaat cgcaaggtgg atctgttccg gttcgatatc ctgaatctga
    14461 ccgcgcatcg gggctgtatc gacatacagg ctacgcagaa tgtctgccgc agcggtgacg
    14521 caaatcggac ggccggtcag tttaaagtgg ttgtcgcggc gattgatctc gatgccgaga
    14581 cggcgttcga gctgcttgat gttgtcatca aacgggccgc acaggctcaa cagacgcgcg
    14641 ttgtctgctg gctccagggt gatttcgcga gtgtctatgt tcaaaccgtt cctcttatct
    14701 gtatgccgcc ggaagctgaa cattcaccgg cctataagga aattattcac gccacaggaa
    14761 aaaggcgcaa gcattgcgat aaagatgggg ataaagagag aaaaaacaag gcccaccgga
    14821 acggcgggcc tgagaattac ggctgataat aacccacgcc aaggtcgttt tctttgcggg
    14881 tacgggcaat caccgattcc ggtgtttctg ccacgcgcag acccatttca tcttcagtac
    14941 gcaccacttt accgcgcaga gagttcgggt agacgtcggt aatttctaca tcgacgaatt
    15001 taccgatcat atccggtgtg ccctcgaagt tgaccacgcg gttattttcg gtacgcccgg
    15061 aaagctccat gatgctctta cgcgaagtcc cttctaccag aatacgctgg gtggtgccga
    15121 gcatccggcg gctccatgcc atcgcttgct gattgatacg ttcttgcaga atatacagac
    15181 gttgcttctt ctcttcttcc ggaacatcat caaccatatc ggcggctggt gtacccggac
    15241 gtgcagagaa gataaagctg tagctcatgt cgaaattgac gtcggcaatc agcttcatcg
    15301 ttttctcgaa gtcttcggtg gtttcgccag ggaagccaac aatgaagtca gaactgatct
    15361 gaatatccgg acgcgccgca cgcagtttac ggatgatcgc tttgtactcc agcgccgtgt
    15421 gagtacggcc catcaggttc agaatgcgat cggaaccgct ttgtactggc agatgcagga
    15481 agctcaccag ctccggcgtg tcgcgataca cttcgatgat atcgtcggtg aattcgatcg
    15541 gatggctggt ggtaaagcga atacgatcga tcccgtcgat cgcagcaacc agacgcagca
    15601 gatcggcaaa cgatccggtg gagccgtcgt agttttcacc acgccaggcg ttcacgttct
    15661 gaccgagcag gttgacttca cgcacgccct gagccgcaag ctgggcaatc tcaaacagaa
    15721 tatcgtcaga cggacggctt acctcttcac cacgggtgta aggcaccacg cagtaggtgc
    15781 aatatttatt gcagccttcc atgatggaga caaacgcggt cggcccttcg gcgcgcggtt
    15841 ccggtagacg gtcaaacttc tcgatttccg ggaagctgat atctacaacc gggctgcggt
    15901 cgccgcgcac ggagttgatc atctccggca gacggtgcag cgtttgcggc ccaaaaataa
    15961 tatcgacata gtgggcgcgc tggcgaatgt gctcgccttc ttgcgatgcc acgcagccac
    16021 cgacgccgat aatcaggtct ggattcttct cttttaacag tttccagcga cccaactgat
    16081 ggaagacttt ttcctgagcc ttctcgcgga ttgagcaggt gttcagcagc agcacatccg
    16141 cttcttccgc cacgtcggtc agttgatagc cgtgggtggc atccagcaga tcggccatct
    16201 tcgatgaatc gtactcgttc atctgacagc cccaggtttt aatatggagt tttttggtca
    16261 tcgacttgct cttgcgaaat agtagccagg aatgcagggc gtatagtgta atgctttgct
    16321 gccgttgtga ccagtatgag cgttatcagc ccttgggggt aaaaatcctg taaacttaaa
    16381 gcatattgct aacaggatga ttgaccatga caaatcaacc aacggaaatt gccattgtcg
    16441 gcggaggaat ggtcggcggc gcgctggcgc tggggctggc acagcacgga ttttcggtaa
    16501 cggtgatcga gcacgcagaa cctgcaccgt ttgtcgctga tagccaaccg gacgtgcgga
    16561 tctcggcgat cagcgccgca tcggtatcat tgcttaaagg gttaggggtc tgggatgcag
    16621 tacaggctat gcgttgccat ccttaccgca gactggaaac gtgggagtgg gaaacggcgc
    16681 atgtggtgtt tgacgccgct gaacttaagc taccgttgct tggctatatg gtggaaaaca
    16741 ccgtcctgca acaggcgctg tggcaggcgc tggaagcgca tccgaaagta acgttacgtg
    16801 tgccaggctc gctgattgca ttacatcgag ataatgatct tcaggagctg gaactgaagg
    16861 gcggtgaaac gatccgcgcg aaactggtga ttggtgccga cggcgcaaat tctcaggtgc
    16921 ggcagatggc gggaattggc gttcatgcct ggcagtatgc gcagtcgtgc atgttgatta
    16981 gcgtacagtg tgagaacgat cccggcgaca gcacctggca gcaatttacc ccggacggac
    17041 cgcgtgcgtt tctgccgttg tttgataact gggcatcgct ggtgtggtat gactctccgg
    17101 cgcgtattcg ccagttgcag aatatgaata tggcgcaatt ccaggcggaa atcgcgaagc
    17161 atttcccgtc gcgtctgggt tacgttacac cgcttgccgc tggtgcgttt ccgctgacgc
    17221 gccgacatgc gttgcagtat gtgcagccgg ggcttgcgct ggtgggcgat gccgcgcata
    17281 ccatccatcc gctggcgggg cagggggtga atcttggtta tcgtgatgtc gatgccctga
    17341 ttgatgttct ggtgaatgcc cgcagctacg gtgaagcgtg ggccagttat cctgtcctca
    17401 agcgttacca gatgcggcgc atggcggata acttcattat gcaaagtggt atggatctgt
    17461 tttatgccgg attcagtaat aatctgccgc cgctgcgttt tgtgcgtaat ctcgggttaa
    17521 tggcggcaga gcgtgctggc gtgttgaaac gtcaggcgct gaaatatgcg ttagggttgt
    17581 agccttacaa cattgccgga tgcgtgccaa ccgtaggtcg gataagacgc ggcagcgtcg
    17641 catccgacat tgaaggataa gacgtgtcaa catcgcattc gacattgaat gaacgcagaa
    17701 aagcaaaaag ctcgccgaag cgagcttttt taatgtggct ggggtacgag gattcgaacc
    17761 tcggaatgcc ggaatcagaa tccggtgcct taccgcttgg cgatacccca actggtgcac
    17821 ttacgatgta agcgtcttga ataaattggc tggggtacga ggattcgaac ctcggaatgc
    17881 cggaatcaga atccggtgcc ttaccgcttg gcgatacccc aacaaattgg tttttgaatt
    17941 tgccgaacgt attcgacaca ttcagaattt ggtggctacg acgggattcg aacctgtgac
    18001 cccatcatta tgagtgatgt gctctaacca actgagctac gtagccagat tgtttcttcg
    18061 atggctgggg tacctggatt cgaaccaggg aatgccggta tcaaaaaccg gtgccttacc
    18121 gcttggcgat accccaataa ccggtcggtg aaccgcttac tcgaagaaga tggctggggt
    18181 acctggattc gaaccaggga atgccggtat caaaaaccgg tgccttaccg cttggcgata
    18241 ccccatccgt acaacgcttt ctggtgaatg gtgcgggagg cgagacttga actcgcacac
    18301 cttgcggcgc cagaacctaa atctggtgcg tctaccaatt tcgccactcc cgcaaaaaag
    18361 atggtggcta cgacgggatt cgaacctgtg accccatcat tatgagtgat gtgctctaac
    18421 caactgagct acgtagccat ctttttttcg cgatacctta tcggcgttgc ggggcgcatt
    18481 atgcgtatag agccttgcag cgtcaacctc tttttcaagg aaaattgctc gaaagtgact
    18541 gtttggttag gttgcgaaca gcgtggcgct atattcgtca attattgttt actttgtgtt
    18601 ttttccccac cctacagcca ttcttttgtc atacaggatg aaattcggaa ttcaacaatg
    18661 gtggtgggaa attaatctat gaaatactgg cctacagtga tgagttgtca aacagtgatg
    18721 tggcaaaccc ggaacatttc cttactgcat atcagaatca acaagcacct caataactga
    18781 aacagccccg gatttcaccg gggctgtttc gcatttctta cttatacgcc gactggtgaa
    18841 cgccaaccgc acgaccagac ggatcgtcca ttttcttgaa cgcctcatcc cactcaatcg
    18901 ctttagcgga agaacacgcg acggacgggc cgcccggcac gcactcagcg gcgctcggaa
    18961 gcgggaacag ttcttcaaag atctcccggt acagatacgc ttcttttgag gttggcgtgt
    19021 tgtacgggaa gcggaagcgg gcagtttcca gttgctgatc agaaacctgc tgcgccgcca
    19081 cttctttcag ggtgtcgatc caactgtaac cgacgccatc ggagaactgc tctttctgcc
    19141 gccaggccac gcttgcgggc aggtatgact caaaacattc acgcaggatg tgtttttcca
    19201 ttttgccgtt accgcacatt ttatcctgcg ggttaatgcg catcgccaca tcaaggaatt
    19261 ttttgtcgag gaacggaacg cgtgcttcca cgccccaggc tgacatcgct ttgttggcgc
    19321 gcgcgcagtc atacatatgc agggccagca gtttacgcac cgtctcttca tgcagttctt
    19381 tagcgttggg cgctttatgg aagtaaagat aaccgccaaa cacttcatca gaaccttcac
    19441 cggacagcac cattttaatg cccatcgcct tgatcttacg cgacattaaa tacatcggtg
    19501 ttgaagcgcg aatagtggtc acatcataag tttcgatgtg gtaaatcacg tcgcggatgg
    19561 catccagacc ttcctgtaca gtgaagtgaa tttcgtgatg caccgtgccc agatggtttg
    19621 ccacttcctg ggctgcttta agatccggtg aacccggcag acctacagca aaggagtgta
    19681 actggggcca ccaggcttca gagcgttcct gatcttccac gcgacgggct gcgtatttct
    19741 tggtgatagc ggaaataatt gaggaatcca gaccaccaga aagcagcaca ccgtaaggca
    19801 catcagacat cagatggctt ttaacggaat cttccagagc ctgacgcagc tcgtttttgt
    19861 cggtcacgtt atctttcact gcatcaaagt cgaaccagtc gcgatgataa taagaacgga
    19921 tttcgccgtc ctggctccac aaatagctcc ccgccgggaa ctctttaatc gtgcggcaaa
    19981 ctggcaccag cgatttcatt tctgaggcca catacagctg accgtgttcg tcatacccca
    20041 tatacagtgg gatgatcccc agatggtcgc gaccaatcag gtaggcatct ttttcgctgt
    20101 cgtacagggc aaaggcaaac atgccctgca agtcgtcgag aaattccggc cctttttcct
    20161 gatacagcgc gaggatcact tcacagtcag accctgtctg gaactgataa cgatcgccat
    20221 attcagcgcg caatgcctgg tggttgtaga tttcaccgtt taccgccagt acgtgggttt
    20281 tttgttggtt gtagagaggt tgcgcccccg cgttaacgtc aacaattgac agacgttcgt
    20341 gagcgagaat ggcgttatcg ctggcataaa taccggacca gtccgggcca cgatgacgca
    20401 tcaggcgtga cagctcgagt gctttcttac gctgctcaac tgcgtctgtt ttgatatcga
    20461 atacgccaaa aattgaacac ataaccttct ccgttaacct ggtatttgtt gcttgttgtg
    20521 tttgcttgtt taaaaaaatg ccgcaaagca gcactgtgcg caagcgattt ggcggtgaaa
    20581 aaataaaaaa cgtaatggtg attgtcgatt ggtgaaaaag ggtctggtgt gaggatgtat
    20641 ttattgatga atcgataatt tttagcgggt tttattgaat ggttatattt tacgggg
//
LOCUS       DAZOZD010000059        20467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZOZD010000059 DAZOZD010000000
VERSION     DAZOZD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.059"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUPB_001719"
     CDS             <1..1133
                     /gene="ypdA"
                     /locus_tag="ACHUPB_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0758558.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUPB_001720"
     CDS             1148..1882
                     /gene="ypdB"
                     /locus_tag="ACHUPB_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0758559.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1895..2752
                     /locus_tag="ACHUPB_001721"
     CDS             1895..2752
                     /locus_tag="ACHUPB_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0758560.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUPB_001722"
                     /pseudo
     CDS             complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="ACHUPB_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUPB_001723"
     CDS             complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="ACHUPB_001723"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0758561.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUPB_001724"
                     /pseudo
     CDS             complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="ACHUPB_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7412..8659)
                     /locus_tag="ACHUPB_001725"
     CDS             complement(7412..8659)
                     /locus_tag="ACHUPB_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR0758562.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8681..9007)
                     /locus_tag="ACHUPB_001726"
     CDS             complement(8681..9007)
                     /locus_tag="ACHUPB_001726"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0758563.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUPB_001727"
     CDS             complement(9227..10192)
                     /gene="glk"
                     /locus_tag="ACHUPB_001727"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0758564.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10396..11652
                     /locus_tag="ACHUPB_001728"
     CDS             10396..11652
                     /locus_tag="ACHUPB_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0758565.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUPB_001729"
     CDS             11767..12093
                     /gene="ypeC"
                     /locus_tag="ACHUPB_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0758566.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12140..13378)
                     /locus_tag="ACHUPB_001730"
     CDS             complement(12140..13378)
                     /locus_tag="ACHUPB_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0758567.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13714..14916
                     /gene="nupC"
                     /locus_tag="ACHUPB_001731"
     CDS             13714..14916
                     /gene="nupC"
                     /locus_tag="ACHUPB_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0758568.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14966..17154)
                     /locus_tag="ACHUPB_001732"
                     /pseudo
     CDS             complement(14966..17154)
                     /locus_tag="ACHUPB_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(17363..17438)
                     /locus_tag="ACHUPB_001733"
     tRNA            complement(17363..17438)
                     /locus_tag="ACHUPB_001733"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17403..17405),aa:Ala,seq:ggc)
     gene            complement(17478..17553)
                     /locus_tag="ACHUPB_001734"
     tRNA            complement(17478..17553)
                     /locus_tag="ACHUPB_001734"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17518..17520),aa:Ala,seq:ggc)
     gene            17774..18133
                     /locus_tag="ACHUPB_001735"
     CDS             17774..18133
                     /locus_tag="ACHUPB_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0758569.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18135..18527
                     /locus_tag="ACHUPB_001736"
     CDS             18135..18527
                     /locus_tag="ACHUPB_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0758570.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="ACHUPB_001737"
     CDS             complement(18579..19994)
                     /gene="gltX"
                     /locus_tag="ACHUPB_001737"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0758571.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20253..20328
                     /locus_tag="ACHUPB_001738"
     tRNA            20253..20328
                     /locus_tag="ACHUPB_001738"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20286..20288,aa:Val,seq:tac)
     gene            20373..20448
                     /locus_tag="ACHUPB_001739"
     tRNA            20373..20448
                     /locus_tag="ACHUPB_001739"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20406..20408,aa:Val,seq:tac)
BASE COUNT         5086 a         5459 c         5085 g         4837 t
ORIGIN      
        1 ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag accaagctgg
       61 cgctggatat cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac
      121 gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata
      181 ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact
      241 tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg
      301 atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag
      361 gggtcgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat
      421 tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg
      481 ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta
      541 atccccattt tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg
      601 ataccgctcg ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa
      661 aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca
      721 ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga
      781 attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta
      841 ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg
      901 tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg
      961 aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt
     1021 tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt
     1081 acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag
     1141 tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg
     1201 gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt
     1261 gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc
     1321 gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt
     1381 gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta
     1441 cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc
     1501 ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac
     1561 cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc
     1621 cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat
     1681 gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc
     1741 attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat
     1801 tctgcgactg aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt
     1861 tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga
     1921 tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg
     1981 cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc
     2041 ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg
     2101 aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa
     2161 caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta
     2221 cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca
     2281 acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga
     2341 gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa
     2401 tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg
     2461 ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat
     2521 cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta
     2581 tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat
     2641 tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg
     2701 gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa
     2761 ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga
     2821 ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt
     2881 catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga
     2941 ctgccagtca accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg
     3001 agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg
     3061 ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt
     3121 gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt
     3181 ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc
     3241 aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct
     3301 atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc
     3361 atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca
     3421 aatgcctacc catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag
     3481 gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc
     3541 atacagatac tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa
     3601 gtggtcgatg atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc
     3661 tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac
     3721 ccataagatc tgatcgaggc tgttaaccat cgggatcatc aactgggcgt tgccgaaact
     3781 ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat
     3841 acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat
     3901 gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag
     3961 tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat
     4021 atagagcatt tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg
     4081 cgcttccaga gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata
     4141 ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc
     4201 gacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc
     4261 gtcaagcact gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag
     4321 taccgggatc gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc
     4381 cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc
     4441 ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg
     4501 ctaggtgata tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag
     4561 ataatcgctg gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc
     4621 cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc
     4681 atcctgaatc agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg
     4741 caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc
     4801 ttgcgcactg gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag
     4861 cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg
     4921 gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc
     4981 gctgtcgata aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc
     5041 gctgccgcta atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc
     5101 cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct
     5161 ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg
     5221 caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct
     5281 gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag
     5341 ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg
     5401 ggtggcaggc cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc
     5461 gtccgtgccg ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac
     5521 ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag
     5581 catcggcccg ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca
     5641 gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc
     5701 gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc
     5761 ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa
     5821 ggctttcccc atcacccgct ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg
     5881 atcgcctgga cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac
     5941 gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca
     6001 ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc
     6061 aaccggcagc acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc
     6121 gtccatatgc gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac
     6181 cgatcccaga ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat
     6241 ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag
     6301 cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact
     6361 tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc
     6421 aaataaatcc ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc
     6481 ggtgaaaagc gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca
     6541 aaatagtcgc caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag
     6601 cagcgtacgc cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga
     6661 tagacgttaa acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg
     6721 cgcgtcatat cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg
     6781 cctgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg
     6841 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     6901 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     6961 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7021 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7081 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7141 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     7201 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7261 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7321 ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc
     7381 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt
     7441 tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg
     7501 cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     7561 ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga
     7621 ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     7681 taatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     7741 acaggcgttt gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg
     7801 gaataacgat agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga
     7861 aggcaacaaa acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg
     7921 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga
     7981 taccgccgtt gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa
     8041 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8101 actggatttt ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg
     8161 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8221 agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg
     8281 ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa
     8341 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac
     8401 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8461 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8521 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     8581 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     8641 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     8701 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     8761 tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt
     8821 ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg
     8881 cgccctgggt ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct
     8941 gagcggccat aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct
     9001 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     9061 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9121 gtgtgacgca ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat
     9181 ttaaaagatt atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc
     9241 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga
     9301 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
     9361 ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa
     9421 ccccaccaaa ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc
     9481 aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
     9541 tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca
     9601 aattcaccag cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac
     9661 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
     9721 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
     9781 ctgttccggc accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact
     9841 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
     9901 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
     9961 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    10021 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    10081 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    10141 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    10201 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    10261 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt
    10321 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    10381 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    10441 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    10501 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    10561 cctatctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    10621 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt
    10681 ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    10741 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    10801 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    10861 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    10921 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    10981 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    11041 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    11101 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    11161 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    11221 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    11281 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    11341 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    11401 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    11461 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    11521 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    11581 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    11641 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    11701 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    11761 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    11821 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    11881 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    11941 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    12001 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    12061 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    12121 tgatgcgacg ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca
    12181 gcgccacgac cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca
    12241 gatcgcccat caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga
    12301 taccaaaact taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca
    12361 gaatgacaat aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga
    12421 agcgaatgaa tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca
    12481 gtccggcagc aaccagactt aacccaaaga ccgttgccgc agcatggctt aacagcggtt
    12541 gcagcgtcag ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg
    12601 ccgccgcagc tgtagccatc atcgccagat tgacaaaacc ggcaatcgtc atggcgatag
    12661 ccacatccca tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag
    12721 tgagcgaaga gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg
    12781 ccaggaagac cgcctccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg
    12841 ccaggttagg ctgggagaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta
    12901 acccgccaat cactttctcc agtggttttt gcccgcgacg ttgcagcatt aaaatcagga
    12961 aagtcgcgat ccccgtcagc accgcgccct gcaacaacga gacaccaaga atgagtttaa
    13021 aaccgatcgc cgcaccaata aattccgcca ggtcggttgc catcgcaata atttctgcct
    13081 gaacccaata gaaccacact acgggacgcg gatagtgatc acgaatctgc tccgccagat
    13141 tttttccggt ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt
    13201 tggcccaaac gacaacccac agtagctgat agccgaagct ggcacccgcc tgaatattgg
    13261 tcgcaaagtt accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg
    13321 cgagcctcat cttgcgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct
    13381 tgtgcctcta aaacatagcc tttgctatgt ttcatgctat gataaacgag aatgattatc
    13441 aaattcattt aaatggattg tggtgatttc tctgatagac caggattatg actacgaaaa
    13501 gattgatgac ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc
    13561 ttccatacac aacatagcag aaatgtatga cagatcacta tttttgaagc ctgtcacagg
    13621 acgtcattat agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac
    13681 ggaaatttaa cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg
    13741 gcacttgccg ttgttgcgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt
    13801 atccgttatg ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac
    13861 tccgacgttg gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcaga
    13921 tttgccaacg aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc
    13981 ttcttcctga aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag
    14041 cacattcgcg tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac
    14101 ggtatgggca aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtctgaa
    14161 aactttattg cctataaaga tatcctcggc aaaatctccc gtaatcgtat gtacaccatg
    14221 gctgccacgg caatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg
    14281 gaaccgaaat acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt tatcgtgctg
    14341 tcgctgatca atccttaccg tgttgatgcc agtgaagaaa acatccagat gtccaacctg
    14401 cacgaaggtc agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaagtt
    14461 gccattatcg ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca
    14521 ctgtttgcca ccgttactgg ctggtttggc tacagcatct ccttccaggg catcctgggc
    14581 tacatcttct atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg
    14641 ggcagtatca tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag
    14701 aaaattgctt ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctggtttcc
    14761 ttcgctaact tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag
    14821 caaggtaacg tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt
    14881 gtgctgtctg cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga
    14941 tcattccccg gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat
    15001 acccttgcag atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga
    15061 caaactccgc gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa
    15121 tcatcgcatc cagcgtattc gtgacaatat ctttcacaaa gacgccatca attttgatga
    15181 tatcagcctg caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa
    15241 tcgcaatccg gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt
    15301 tagaaaacgc ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac
    15361 gcttaaacag acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta
    15421 aattgactga aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca
    15481 aggattccag cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca
    15541 gaaacttatc cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat
    15601 aaccttcacc ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta
    15661 acgccgtgcg aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct
    15721 cctctctgct atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc
    15781 ttaactgccc caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag
    15841 caccatagcc catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt
    15901 taaccatatg ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac
    15961 tacccggcaa ttgatataac ttttcgtttt cctgcatcaa cggcagcagc gtacggtaaa
    16021 ttgatcggat acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat
    16081 tatcaatgcg caggcagcaa aactcttgcc cgcctcttgc tccggcgctt gttccaacgc
    16141 acggaagttg ggcaggagcg ttaacggatc ggtcagcgcc tgcaaatgcc agcggcgatt
    16201 aagccattca ctgcgatgat aaatgcgcac catatagagc aggcaaacgc taaaggaaat
    16261 cagcaccgca agaataaatg ccagcgaata ttcggtttcc accccttgca aaaagttctg
    16321 gttgtaattc agaaggcaaa gcgttgaaac agccctggtg agatttaaaa acggatagcg
    16381 aagcttaccg accccgaggg taaagatgat gaagaaaacg ggtaccaggt aaccggcaat
    16441 aaagtcgttt tcataaggtg tgcacaacag aagtagaagc acgcttaacg ctgccagcca
    16501 gcttaaggta aatgcgcgtt tctctttgcc caacgacggc gcgatatccc tgcgccacaa
    16561 tatctgcgca aagtggggac ttacaatcat gcgagtgaga tagtagaaaa gcatgttgta
    16621 aataagcaca gcggtgaaaa ggcttagcaa atcaacgacc gtgaaaatgg catccgcatc
    16681 gccgaaaaag gtggatatct ttagcgggaa atcaaagaag ctccccacaa gatacatgct
    16741 gcatttgatg ccgatcggcg tcaccaaacc aagccagaac agacgttgcc agacatatcg
    16801 gctggtcaat ccataacgcc aacgcgtccc cagctgccag cgtaatattg cgcaggcaca
    16861 caacacgaca aacgtctggc taccgagcat gaccgcagtc tgtaaaaaag agagatgaaa
    16921 gttccacaga ttggtgcaaa acatcccgca taaaacggga acgacaccgc gccagccaaa
    16981 gataaacaag attgacagca ttacgcacag tggcatccac gccagaaaga tataactgga
    17041 atggataatg gcaagtggcg aaataaaacg ggatagctga ataagtacca cggtgagcgt
    17101 aaacgctagt gtgaatatct tgatattttt tatcaggtta tgctccacaa acatgagtac
    17161 actactctca ctgatcgggg ttttcaggca cacaaataat aagtttattc gcatcgcgaa
    17221 gcaaacaggg ggccagcctg ttgccgattt ttcatccaat gatgctttca ccaggcggca
    17281 ctaacccgga cgtagactgt attacaaaag cggcaaaaag cagagacaaa aaacccccgc
    17341 tttgcagcga gggttggaaa tttggtggag ctaagcggga tcgaaccgct gacctcttgc
    17401 atgccatgca agcgctctcc cagctgagct atagccccac gatgcgttta cgtaccaaat
    17461 ttgctgggtg caaaatttgg tggagctaag cgggatcgaa ccgctgacct cttgcatgcc
    17521 atgcaagcgc tctcccagct gagctatagc cccgtcacgt aaagcttgtc gagttgacgg
    17581 gcggcatcat atgaattccg cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa
    17641 tcgctgaaaa atcaggcaaa tgaacaattt tggaaaaccg ctcgcattca gtagttattc
    17701 atgtcacggt ttcctgtaaa gtggtgttat aaaatgaact actaatagac ccacatacat
    17761 tcagggaatt gttatgttca aggagaggat gacaccagat gaacttgcca gactgaccgg
    17821 ctatagccgc cagaccatta ataaatgggt acgcaaggag ggctggacaa cgtcaccaaa
    17881 acctggcgtc cagggtggca aagcccgact ggttcacgtc aatgaacagg ttcgtgaata
    17941 tattcgcaat gctgaacgtc cagaaggtca gggagaagcg cctgcccttt ctggtgatgc
    18001 gccgcttgaa gtattgctgg tgacactggc gaaagagatg acgccagttg aacaaaaaca
    18061 gtttacatcc ttgcttctgc gggaagggat tatcggattg ttacaacgct tagggattcg
    18121 cgatagcaaa taatatgaaa agattacgca ataaaatgac caccgaagaa ctggctgaat
    18181 gcctcggtgt ggctaaacaa actgttaacc gttggatcag agaaaaaggc tggaaaacgg
    18241 aaaaatttcc cggcgtgaaa ggcggtcgtg ccagacttat tctggtcgat acgcaagttt
    18301 gcgagtttat tcagaacacg ccagccttcc ataacacacc aatgttgatg gaagctgaag
    18361 agagaattgc tgaatacgct cctggagcac gcgccccggc gtatcgacaa atcatcaacg
    18421 cgatagataa tatgacagac attgagcagg gaaaagtcgc gcaatttttg tcacgtgaag
    18481 gaattcgcaa tttcctcgcc cgtctcgata ttgacgaatc agcataaata aaaaacggca
    18541 ggatattatc tcctgccgtt tatcttttta cacgctaatt actgctgatt ttcgcgttca
    18601 gcaataaaat ccagcgcttt gttgatacgc tcgatactgc gggtcttacc aattgcgtga
    18661 acggtaacat ccagtgctgg agactgcccc gccccggtta cggcgacacg cagcggcata
    18721 ccaactttac ccatacccac ttccagctca tccgccgtcg cctgaatagc gtgatgaacg
    18781 ttttcagcgg tccagtcagt aatcgcggcc agtttgtcac gaaccacttc cagcggctga
    18841 cgcgctaccg gacgcagatg ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg
    18901 tagaaataac ggcagctctg tgccatctct ttcagcgtct tgcagcgttc gcccagcagt
    18961 ttcaccagat cagccagctg cgggccgtta cgggtatcga tattttcctg ctcaatgtgc
    19021 cactgtaagt gagtagcaac atactccggc ggcagcgcgt taatgtagtg atggttcagc
    19081 cacagcagct tgtcggtgtt gaacgcactg gcagatttgc tgacggcatt caaagtgaag
    19141 tatttgatca tctcttcacg agtgaagatt tcctgatcgc cgtgggacca gcccagacgc
    19201 accagatagt tcagcagtgc ttctggcaaa taaccgtcat cacgatactg cattacgctg
    19261 actgccccgt gacgtttgga cagtttttta ccgtcatcgc cattgatcat agaaacgtgc
    19321 gcgtaaaccg gcaccggcgc tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg
    19381 ttgatatggt cttcgccacg gataacgtgg gtgatttcca tatcccagtc atcgacaacc
    19441 acacagaagt tataggttgg ggaaccatcg gtacggcgga taataagatc gtccagttcc
    19501 tggttgctga actcgatcgg accacggatc tgatcgtcaa aaacaacaga accttcctgc
    19561 gggttagcaa aacgtacaac acacggttca tcatcagcat gatgctcatg gctgtggcgg
    19621 cagcgaccgt cataacgcgg cttctcacct ttcgccattt gctcttcgcg cagcgcttcc
    19681 aggcgctctt tagagcaata gcatttataa gcagtgccct cttccagcat ctgatcgatc
    19741 accgcgttgt agcgatcaaa acgtttggtc tggtagtacg gaccttcatc ccactccagg
    19801 ctcagccagt tcatgccatc cataatggct tcgatagctt ccggcgtgga acgctcaaga
    19861 tcggtgtctt caatacgcag cacgaactca ccgccgtggt tacgtgcaaa aagccaggag
    19921 taaagagcag tacgcgcgcc gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga
    19981 gttttgattt tcatgaaatg gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg
    20041 gaaaaattga gtgggcgata ttctatcact cccgcctgat tcctcaatgt agttcgggtt
    20101 tctaccttac gcattattgt tttcgttgac aaattgcgca acacggtctg ttttgcgatc
    20161 atcttgctta aatttacgac gaacgaacaa tttctttaga aaatgcgttg actcattttg
    20221 aactctccct ataatgcggc tccacacagc gggggtgatt agctcagctg ggagagcacc
    20281 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaac tactttatgt
    20341 agtctccgcc gtgtagcaag aaattgagaa gtgggtgatt agctcagctg ggagagcacc
    20401 tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccaat ttctcgccag
    20461 ctaaatt
//
LOCUS       DAZOZD010000060        20399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZOZD010000060 DAZOZD010000000
VERSION     DAZOZD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.060"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(130..1779)
                     /gene="actP"
                     /locus_tag="ACHUPB_001740"
     CDS             complement(130..1779)
                     /gene="actP"
                     /locus_tag="ACHUPB_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0758572.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1776..2089)
                     /locus_tag="ACHUPB_001741"
                     /pseudo
     CDS             complement(1776..2089)
                     /locus_tag="ACHUPB_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2289..4247)
                     /gene="acs"
                     /locus_tag="ACHUPB_001742"
     CDS             complement(2289..4247)
                     /gene="acs"
                     /locus_tag="ACHUPB_001742"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR0758573.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4641..6077
                     /gene="nrfA"
                     /locus_tag="ACHUPB_001743"
     CDS             4641..6077
                     /gene="nrfA"
                     /locus_tag="ACHUPB_001743"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0758574.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6122..6688
                     /gene="nrfB"
                     /locus_tag="ACHUPB_001744"
     CDS             6122..6688
                     /gene="nrfB"
                     /locus_tag="ACHUPB_001744"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0758575.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6685..7356
                     /gene="nrfC"
                     /locus_tag="ACHUPB_001745"
     CDS             6685..7356
                     /gene="nrfC"
                     /locus_tag="ACHUPB_001745"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0758576.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7353..8309
                     /gene="nrfD"
                     /locus_tag="ACHUPB_001746"
     CDS             7353..8309
                     /gene="nrfD"
                     /locus_tag="ACHUPB_001746"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0758577.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8329..10047
                     /locus_tag="ACHUPB_001747"
     CDS             8329..10047
                     /locus_tag="ACHUPB_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0758578.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10040..10423
                     /gene="nrfF"
                     /locus_tag="ACHUPB_001748"
     CDS             10040..10423
                     /gene="nrfF"
                     /locus_tag="ACHUPB_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0758579.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10420..11016
                     /gene="nrfG"
                     /locus_tag="ACHUPB_001749"
     CDS             10420..11016
                     /gene="nrfG"
                     /locus_tag="ACHUPB_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0758580.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11358..12671
                     /gene="gltP"
                     /locus_tag="ACHUPB_001750"
     CDS             11358..12671
                     /gene="gltP"
                     /locus_tag="ACHUPB_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0758581.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12750..13439)
                     /gene="yjcO"
                     /locus_tag="ACHUPB_001751"
     CDS             complement(12750..13439)
                     /gene="yjcO"
                     /locus_tag="ACHUPB_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0758582.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="ACHUPB_001752"
     CDS             complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="ACHUPB_001752"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15261..15263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR0758583.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="ACHUPB_001753"
     CDS             complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="ACHUPB_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR0758584.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="ACHUPB_001754"
     CDS             complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="ACHUPB_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0758585.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="ACHUPB_001755"
     CDS             complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="ACHUPB_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0758586.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5435 c         5601 g         4808 t
ORIGIN      
        1 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
       61 ttgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc gaccggggaa
      121 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      181 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      241 gaaccagatg ccgaggaatg ccacggtgat agagaacagc gccgggtatt catacgggaa
      301 gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga tcatcagtac
      361 tactgcggta atcagcccca gccagccacc catcatcgcg ccacgcgtgg tcagtttcga
      421 ccagtacata gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgccag
      481 ccccaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      541 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      601 tttgaagacg ttagcgtaca agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc
      661 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt
      721 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      781 cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga agccgataat
      841 aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc tcttacgtgc
      901 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
      961 aaacatcagt cccagaccca gagagagtgc ggagatcgga tctttcacca gcccgcccgg
     1021 cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca gattgttgaa
     1081 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1141 cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca ggacgtacat
     1201 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1261 gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca ccaccagaga
     1321 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg ccacatcggc
     1381 aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca
     1441 gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa acaccagcgc
     1501 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1561 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgagttttga
     1621 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1681 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1741 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1801 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1861 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1921 ggattcagcg gcgtgccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat
     1981 aaactgccag cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact
     2041 ccctgaaatg cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc
     2101 cagaggtaat gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg
     2161 ggtaaccctt gccgaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaatgc
     2221 ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc gcatccggca
     2281 attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt ctcgactacg
     2341 ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg
     2401 cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc ggtccagtgc
     2461 agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg gacttctgcg
     2521 tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga
     2581 cctttaatat tgtgcggaat acctactacg gcggcttcgg caatcttcgg atgcgccacc
     2641 agcgccgact caatctctgc cgtccccaga cggtgaccgg agacgttcag cacgtcgtcc
     2701 acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa
     2761 tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac gttcgtgatc gccaaacagc
     2821 gtacgcgcct gacccggcca ggagtcggtg atcaccaggc tgccttcggt agcgccttcc
     2881 agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc
     2941 gaaccggctt tcagctcggt agcgccaggc agcggcgtga tcatgaaacc gccagtttcg
     3001 gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt ccagtaccac
     3061 tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag cgacgaacgg
     3121 tcggtaccct cgatcgcttt atcgccttcc gccatcagcg cgcggatcgc cgtgggtgcg
     3181 gtatagagaa tattgacctg atgcttgtcc accacctgcg ccatacgggc aggcgtcggc
     3241 cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc aggccagcgg gccgtacagc
     3301 aagtaactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat atcacccgga
     3361 tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc gccggtagta
     3421 tgcagcacac cttttggctt accggtagaa ccggaggtgt agagaataaa cagcggatct
     3481 tcggcgttca tcttttccgc ctggtgctga tcgctcgctt gctcaaccag gtcgtgccac
     3541 cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag taccaccaca
     3601 tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat caacgttttt cttcagcgga
     3661 atactgcgcc cggcacgcac accttcgtcg gaagtgatca ccagtcgtga gttggaatca
     3721 ataatgcgcc cggcaacggc ttccggcgag aagccgccga aaatcaccga atgcaccgcg
     3781 ccaatgcggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg catataaatc
     3841 gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa gcggcagacg
     3901 tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc gccttcccag
     3961 atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt tgccgccaga
     4021 ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa ggaggtgttt
     4081 ttcaccttct ggtaaggttt gatccagtca agaatttttc cctgttcgcc ccagaaggta
     4141 tcaggtacgt taatagattg ttgatacatc gcctcgtact gctgagggtt tatcaggcaa
     4201 cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt ggctcatgct tttgttctcc
     4261 ttgtagggtg ttaataatat gtggcataag cgttaaatgt aggggtattg gcagttttgt
     4321 ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg gcagggtaat
     4381 ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt tatgcattgc
     4441 tgttgaaaaa gaggaagata ctaactaact ctaaagtggt attttacatg cacttacaat
     4501 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat
     4561 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg taacaatggc
     4621 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4681 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4741 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4801 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4861 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     4921 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     4981 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5041 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5101 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5161 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5221 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5281 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5341 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5401 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5461 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5521 aaggcaaact ctataccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5581 gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5641 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5701 cgaaagcagc gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata
     5761 tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg
     5821 caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     5881 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     5941 tctcaaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6001 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg
     6061 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6121 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag
     6181 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6241 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6301 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6361 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6421 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6481 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6541 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6601 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6661 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6721 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6781 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6841 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6901 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     6961 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7021 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7081 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7141 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgtgt tgaggcttgc
     7201 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7261 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7321 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7381 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7441 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7501 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7561 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7621 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7681 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7741 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7801 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7861 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     7921 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     7981 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8041 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8101 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8161 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8221 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8281 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8341 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8401 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8461 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8521 ctcatcagcg atttctcggt catttatgtc gcgcaacata gccatagcct gctgtcgtgg
     8581 gaattcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8641 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8701 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8761 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8821 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8881 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     8941 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9001 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9061 gatccggtgg aaaatgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9121 ttatccctga cacgccagcg ggggattttt tgccactggt cgctgttgct ggcgatagtt
     9181 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9241 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9301 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9361 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9421 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct gggctgggga
     9481 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9541 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg
     9601 gtcgctcatg cgggcgtgct gttatttgcc gcagggatcg tggtttccag cgtcagccgt
     9661 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9721 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9781 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9841 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9901 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta tgtacaaagc
     9961 ggtgtgcgct ggatctgggg tggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10021 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10081 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10141 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10201 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10261 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10321 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10381 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10441 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10501 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10561 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10621 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10681 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10741 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10801 cagacttgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10861 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10921 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    10981 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11041 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat
    11101 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11161 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11221 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11281 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11341 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11401 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11461 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11521 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11581 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11641 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11701 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11761 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11821 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11881 cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    11941 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12001 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12061 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc
    12121 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12181 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12241 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12301 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12361 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12421 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12481 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12541 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12601 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12661 cggatcaata attgaagatt gccggggata tccacccggc aatgtgtgaa tgcctgatgc
    12721 gacgcttgcc gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca
    12781 cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg
    12841 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    12901 taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    12961 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    13021 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatagcctt tggataatcc
    13081 ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    13141 cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    13201 ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct
    13261 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    13321 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    13381 gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt
    13441 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    13501 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    13561 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    13621 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    13681 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    13741 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    13801 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    13861 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    13921 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    13981 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    14041 ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac
    14101 tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga
    14161 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    14221 gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct
    14281 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    14341 gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    14401 acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    14461 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    14521 ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg
    14581 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    14641 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    14701 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    14761 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    14821 tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    14881 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    14941 gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc
    15001 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    15061 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    15121 tgattcgcca cgattgggtg ggaatcagcc gggttgtacc cgaaaacgaa cactaaatcg
    15181 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    15241 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    15301 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    15361 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggta
    15421 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    15481 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca
    15541 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    15601 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    15661 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    15721 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    15781 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    15841 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    15901 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    15961 tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct
    16021 gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg
    16081 ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc
    16141 cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca
    16201 tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    16261 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    16321 ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    16381 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    16441 ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta
    16501 atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag
    16561 attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    16621 tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct
    16681 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    16741 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    16801 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc
    16861 gatgcacacc ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat
    16921 agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt
    16981 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    17041 catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg
    17101 tgtgtgaacc acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt
    17161 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    17221 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt gcgtaccaag gcacagccgg
    17281 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga
    17341 tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    17401 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    17461 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    17521 cagaccggca gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    17581 tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg
    17641 gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag
    17701 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    17761 accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg
    17821 cattacttta cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    17881 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    17941 cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt
    18001 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    18061 ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt
    18121 gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc
    18181 cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat
    18241 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta
    18301 acagatcaaa caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa
    18361 agcatcggcg accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat
    18421 ctggcccagt tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac
    18481 cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg
    18541 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    18601 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    18661 gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac
    18721 attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    18781 gctgtctgtc aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg
    18841 cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata
    18901 gaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat
    18961 ggcggggaag gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag
    19021 ccgatgggtg cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg
    19081 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19141 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19201 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag
    19261 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19321 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt
    19381 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19441 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19501 ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    19561 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19621 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19681 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    19741 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    19801 atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19861 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19921 agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca
    19981 aaaccctctt tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg
    20041 gcacgggctt tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg
    20101 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    20161 agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc
    20221 tggttgtcgg tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg
    20281 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20341 aaaacaaggg caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagg
//
LOCUS       DAZOZD010000061        20203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOZD010000061 DAZOZD010000000
VERSION     DAZOZD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.061"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(199..471)
                     /locus_tag="ACHUPB_001756"
     CDS             complement(199..471)
                     /locus_tag="ACHUPB_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758587.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIEVMQEQAVKAFVSWMTNQEG
                     TEFLSTLQEITTELDALIDAVESHSPVFRKKATELEALLAHYKTLL"
     gene            complement(1430..2095)
                     /locus_tag="ACHUPB_001757"
     CDS             complement(1430..2095)
                     /locus_tag="ACHUPB_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="HFR0758588.1"
                     /translation="MAKQSPCYNVVSRKRRRFLNRWIKSLTTVAGRIKIKNELRRRIR
                     HNRYWVNEANKFGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGHKS
                     ISRASRGCDRLVWLNAIITEKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERA
                     RLLKADPDEVIYSGDVRLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHSPTP
                     IPA"
     gene            complement(2712..3077)
                     /locus_tag="ACHUPB_001758"
     CDS             complement(2712..3077)
                     /locus_tag="ACHUPB_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0758589.1"
                     /translation="MVPKRLKEARMEAGLSQEKLSQLLGVAEGINTRSRFSSYEVGRT
                     EPPFKLVKKIAELLDYPENYFYTEDDDFAKAVLDFHRKRTSPELNPNYDSEVEARRMA
                     SKLDNVKKIVAQLNEYLKE"
     gene            3332..3781
                     /locus_tag="ACHUPB_001759"
     CDS             3332..3781
                     /locus_tag="ACHUPB_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0758590.1"
                     /translation="MNTWYLLYCNNNDLDRIAYRCKTMNVVAFCPRYIKITPRKDCNA
                     VRKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPEKVVIAIKCAK
                     ILALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
     gene            3858..4070
                     /locus_tag="ACHUPB_001760"
     CDS             3858..4070
                     /locus_tag="ACHUPB_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758591.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAF
                     LQYMIDKLSNEAHNEMLKELMSKEDK"
     gene            4071..4277
                     /locus_tag="ACHUPB_001761"
     CDS             4071..4277
                     /locus_tag="ACHUPB_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758592.1"
                     /translation="MPKPTYNVYVTQEVPTDENGGKNTYWTKVGAAFAHNGKPGLNIM
                     LVPGIAVSGKLVLLEPKEDAPQGN"
     gene            4293..4997
                     /locus_tag="ACHUPB_001762"
     CDS             4293..4997
                     /locus_tag="ACHUPB_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0758593.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPK
                     RERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLF
                     NPCTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSP
                     EKPRYVVPGTRDDIATQSAILNATPVEAPARPRVVWPGAIVRGVPAQLRQKKADTVYY
                     PAQVIRGNRFSTEKED"
     gene            5001..5297
                     /locus_tag="ACHUPB_001763"
     CDS             5001..5297
                     /locus_tag="ACHUPB_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HFR0758594.1"
                     /translation="MFNKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVS
                     KGLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMN"
     gene            5310..8054
                     /locus_tag="ACHUPB_001764"
     CDS             5310..8054
                     /locus_tag="ACHUPB_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFR0758595.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFL
                     PIEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDA
                     MKLNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLH
                     GLIRSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFV
                     TICYRPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTE
                     NEAVFSSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFR
                     GLEVKEFSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDA
                     VSQRLDLDVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVP
                     AEISLSAAYMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVR
                     TPSGDGYYLNLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDS
                     FSPDARTKRLTTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFI
                     KQLMKILCTRNGATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQ
                     ENGLSIRLSQWARGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLL
                     YRITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIK
                     DEIAPAVIEQCGTQILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQF
                     RRGDTKRFAARITLDLSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL
                     "
     gene            8069..8776
                     /locus_tag="ACHUPB_001765"
     CDS             8069..8776
                     /locus_tag="ACHUPB_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR0758596.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQ
                     WLQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLS
                     GGSSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLG
                     EINELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMSVKAAEKREKVLEAERV
                     KQWNQQQLTAPTVDLNS"
     gene            8790..9017
                     /locus_tag="ACHUPB_001766"
     CDS             8790..9017
                     /locus_tag="ACHUPB_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR0758597.1"
                     /translation="MRLLTIYLLALSVFLISGCKEEAKTTKWYKDHPEELKAVYEKCQ
                     KTGSASENCKNANEAHWQIQQLNAPTVDLNS"
     gene            9028..10098
                     /locus_tag="ACHUPB_001767"
     CDS             9028..10098
                     /locus_tag="ACHUPB_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR0758598.1"
                     /translation="MSGGVFVGVEKYLIGGITDATKGLMMSYSSMMMGLAAASATIYI
                     MWRGYQTLAGKLSTPMEDTMWDIMRMAIILSFVANLGGYLDGVIDAVNGLKEGFSGSD
                     NIWQLLDSLWNNAKVLGKTLHDLDNSKYIKDEGMTAQFYVWLGIFILMIITAFVSMIA
                     EVMILLLSITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTILLSSLSLRIVVNYLNSR
                     LALATQGAAQANIVELGAQVCLAGVCAAILVYISAKLAGALAGASATATMQGAAALGI
                     GAAAYGAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEA
                     RMLTDQSTPKLPPSIKCLPPPR"
     gene            10147..10290
                     /locus_tag="ACHUPB_001768"
     CDS             10147..10290
                     /locus_tag="ACHUPB_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758599.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPPAFSGQGSPVNTPQIIAELT
                     THD"
     gene            10283..10966
                     /locus_tag="ACHUPB_001769"
     CDS             10283..10966
                     /locus_tag="ACHUPB_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFR0758600.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLIAILSLI
                     AIVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYV
                     YPALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATA
                     PDKVATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQ
                     TDREIRGGE"
     gene            10966..11871
                     /gene="virB9"
                     /locus_tag="ACHUPB_001770"
     CDS             10966..11871
                     /gene="virB9"
                     /locus_tag="ACHUPB_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HFR0758601.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTV
                     VNTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTT
                     QVVIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKE
                     AAARQKEWEQKQEQTQIQKALRTAQTPRNWNYTKYPGKDSYSIEPDFAYDDGRFTFIG
                     FSPNKSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNG
                     FGKVHAADNSTVSDKVERVEKTDNT"
     gene            11913..13163
                     /gene="virB10"
                     /locus_tag="ACHUPB_001771"
     CDS             11913..13163
                     /gene="virB10"
                     /locus_tag="ACHUPB_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="HFR0758602.1"
                     /translation="MTDENKSPSTEEEKPVTTSDQDDIATLEREARAKREAELQASQD
                     DEKENSLQPAVNKLKKRQKGKAAAFLAICAVALIGLTWGGNWVYQNFLKQEDGEKTKE
                     VTPPKKTNDYRKRTDLGMATNEPENSTEGEQTVNQDNTGNVAGTAGQMKTFEPPQLNK
                     ASFLVRRNNTASPQNQVKTRQQEMILAAENNATQPTDSTATTTQHGTASQAISPATVR
                     RIPYNPDLYIPENTVIPCSMDYRFVSDRAGRIRCTVAKDIWSASGNTKLIEKGTTATG
                     IYQTGITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERF
                     GGALMVGMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKN
                     QGEIINLITGQDIDFSNIYTLRMK"
     gene            13167..14192
                     /gene="virB11"
                     /locus_tag="ACHUPB_001772"
     CDS             13167..14192
                     /gene="virB11"
                     /locus_tag="ACHUPB_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFR0758603.1"
                     /translation="MTVKNISLDRYKQRFFSEYLELPGLTEIAVNRPGELWTKIDGVW
                     GQHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTIS
                     ITIRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYG
                     KTLAVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSD
                     EKGSVVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHE
                     AIDGFITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNVRRMNDIYFRPLHRNQ
                     YYEEMKV"
     gene            14189..14587
                     /locus_tag="ACHUPB_001773"
     CDS             14189..14587
                     /locus_tag="ACHUPB_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HFR0758604.1"
                     /translation="MIKHILVTVLVTLITGCSSPPPPVPVEWNKKPVSLNEKLPEWNP
                     NSLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSAKNW
                     LYHHGANGVIIYHPITNCLTCNETTIYFSR"
     gene            14616..14915
                     /locus_tag="ACHUPB_001774"
     CDS             14616..14915
                     /locus_tag="ACHUPB_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFR0758605.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGE
                     CRSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
     gene            14918..15133
                     /locus_tag="ACHUPB_001775"
     CDS             14918..15133
                     /locus_tag="ACHUPB_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758606.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVCSIPSGMKGNFITSIGETL
                     EDLKKRYPDIILITESEALALQSKKEN"
     gene            15161..15466
                     /locus_tag="ACHUPB_001776"
     CDS             15161..15466
                     /locus_tag="ACHUPB_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="HFR0758607.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQ
                     ARLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAAIKAEDECLTEITRAQV"
     gene            16239..17966
                     /locus_tag="ACHUPB_001777"
     CDS             16239..17966
                     /locus_tag="ACHUPB_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR0758608.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNL
                     PREERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITI
                     LFTLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPL
                     DYIDLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGE
                     AVFVEDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAG
                     FSLSKKGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELE
                     EIALVNSVNAKNGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRI
                     NTTPRVVAIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSV
                     IGAVIENAKFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIR
                     QAESYYVDSNMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHN
                     NNDMPSSGL"
     gene            17978..18712
                     /gene="mobC"
                     /locus_tag="ACHUPB_001778"
     CDS             17978..18712
                     /gene="mobC"
                     /locus_tag="ACHUPB_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="HFR0758609.1"
                     /translation="MLIHDFHERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKK
                     SRNPVYKLLNKALKEGFIIKHEIPSSKRKLTVWGITMQGLAMVVKADDITFPSYFEPS
                     KLKYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGIIKQPTGAI
                     MAIETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVM
                     RNNTPVPFDEKNREMFIFRTIDELEQ"
     gene            18716..19180
                     /locus_tag="ACHUPB_001779"
     CDS             18716..19180
                     /locus_tag="ACHUPB_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0758610.1"
                     /translation="MKILIIFALYILSSSLYANTADIQGKVTRVLDGDTIEILQNKTP
                     VRIRLANIDAPEKKQAYGRWSTDQLKSLIAEQSVRVTYSHNDRYGRIIGRVFTTGGSE
                     ANRFMVQSGAAWVYEHYNTDNDLATLQQEARSQKRGLWADPNPVPPWEWRHK"
     gene            19190..19495
                     /locus_tag="ACHUPB_001780"
                     /pseudo
     CDS             19190..19495
                     /locus_tag="ACHUPB_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19674..19937
                     /locus_tag="ACHUPB_001781"
     CDS             19674..19937
                     /locus_tag="ACHUPB_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0758611.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPDNDLAKITKSLRTAENRMVCVIQ
                     DNNRILRWDRSYASRAKNHWRKVAPDRFEILGTVEHIHYVGKC"
     gene            complement(19969..>20203)
                     /locus_tag="ACHUPB_001782"
     CDS             complement(19969..>20203)
                     /locus_tag="ACHUPB_001782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758612.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVD
                     MEVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
BASE COUNT         6279 a         4350 c         4391 g         5183 t
ORIGIN      
        1 tcggtaaata ctacaatata ttttttacca gtctctatat gaattaatcc aatagtacaa
       61 agtgtcaaag tattaaaaat aaaaatctgt tgcgtggttg attcaatata aggtaagcga
      121 gccattttta aactccactt gctttagatt tggattaaat aaagagccat ataacggggc
      181 aattgccccg ttgaggactt acagcaacgt tttgtaatgg gctaaaagcg cttccagttc
      241 agtcgctttc tttctgaaaa caggggaatg ggactctacc gcatcaatca gtgcgtcaag
      301 ttctgttgtg atttcctgca aagtgcttaa aaattctgta ccctcctggt tggtcatcca
      361 ggaaacaaag gcttttactg cttgttcctg cataacttca atttgggtaa tagcgtcttt
      421 ttgctgatcg ccagttgctt taaatgtggt tctaaaaata aatgcactca ttttaatctc
      481 ccgcccctat ggggctaagt ggaaagggaa cctatttccc tttcgatgat tttattatac
      541 ctgcatacgc aggtattgca actatttttc actattcgaa ctgaaaaatt ttccaccact
      601 cacctttaat tccttcgcgt atgtgctcaa ttcagcaagg gcactattcc gcttcctcac
      661 ccactcgctc actaacgttc ggtcgctagg gttgcggcga gcggatctac ctccctaggg
      721 ttgtacaccg gaaacgccga aatttccgat ttccaaactg ccccccaaca ggaacctttt
      781 tgtgatcttt ctcgatcata ttgttagcag ttagttaagt tatgctcgat ggtgggaaga
      841 tgccgcgtta gcggcatgta aggcgcgaaa tatagttcca tagaatcaca aatgctaagg
      901 cgcgccgata cccctaatca aagacgttca tgtcgccagg aaccgcgtca gccccctgct
      961 aagccaaaat tgcacggaac ttacagctac ttaggtggta ggtggtcgta gaccgtcgat
     1021 gttacagcga tgataggtga atgattgttc gtttttaatg accggatatg gtgtctctaa
     1081 tgccccgatg cttgcgaggg gtgctacctt tgaggcgctt gcgccgatgc ttttcagtta
     1141 tccacttgtt aaaggggata acagtaatgt ttttcttttt gcttctcttt tagtacgtaa
     1201 gtaactaaaa gagaagcaaa agagtaattg cgtcatatcg tttgcacttc acttatgatt
     1261 atccacaagc tgtacatcgt ttaccctctg gccataacct atggctgctc tttcctgagc
     1321 agggagccgc ttggaaaacg gaaaatatct cacgctaaga ctattttttg tacagagatt
     1381 tattatggac gtgacgatat accgcccata aggcggtaag tagccagcat caggccggaa
     1441 tgggggtagg tgaatgtgcg taatattctt ttttgcgttg tcgagctgcg tcgcgttttg
     1501 ctttcatcct ggctaatccg gctgccgctt tccgcgtcca gttctggact ctgatagcta
     1561 taagtctgac atcaccggag taaatcactt catcaggatc tgcctttaac agcctggcac
     1621 gttcccgata aaccagtttt aacggaatgc caaggatggc aaagaaatcc tcggttactt
     1681 caatgtgttt acaggcacac cgtgcatcat acgggttaaa cggcgctttc tctgtaatga
     1741 tggcattcaa ccaaaccagg cgatcacaac cacgactggc gcgagaaatg gatttatgcc
     1801 ccgcatcgct gcgtgtaacc agtccggcac gttcggcaag cgtttcaagg ttatgcctgg
     1861 tttcccacga gcgtaaatcg agatcgtcca ggatagctaa catcaattcg caaagagcct
     1921 ctatgccaaa cttgttagcc tcattaaccc aatagcggtt atgccggatg cggcgacgga
     1981 gttcgttttt gattttgata cgccctgcga ccgtcgtcag cgatttaatc cagcgattga
     2041 ggaaacggcg tcgcttacgc gacacaacgt tatagcatgg actttgtttt gccacacctg
     2101 acggcgatat gcgggggatt aaggagttgt tcttttcagc caccaccgcc cgcacttatt
     2161 gccggacaga ggtcttgtat tgagaaatgc atttgctata atcctttgca ggcacccagc
     2221 ctgctttgga ccccctgatt atcttcttgg accggcaaaa tcgaagagag atatcagggg
     2281 gttgttgcat tcaggggggc taaaatgctt cccccccttc ccctggacgc tgttacggga
     2341 acgtcccacc caaaaatccg taaaaaccgc acgaaaatag catgaaccca ttcgaagatc
     2401 aattcatcca caaaagatcc cgccgattac cttcctattt ttctttaaac ttcatctggt
     2461 tatataattc ttaacaaaat tgagattaaa taccgtacag catataactc acgatattaa
     2521 tgctaatgta cgcatacatt agcattaata tcgaaaagaa atcggcgata acaaacggaa
     2581 aaataacgga atttaataga gtatttaccg ctcgccacaa ccctagcgac cgaacgttag
     2641 tgagcgagtg ggtgaggaag cggaaaagcg acttgaccaa atggagcaca tacgcactga
     2701 tgcctaagca ctcattcctt tagatattca tttaattgag caactatttt ttttacgtta
     2761 tccagcttag atgccattct tcttgcttct acttccgaat cgtaattagg gtttagctct
     2821 ggactcgtac gttttcggtg aaaatcaaga acagcctttg caaaatcatc gtcttctgta
     2881 tagaaataat tttcaggata atcaagaagt tctgcgattt tttttacaag tttaaaaggt
     2941 ggttcagttc tgcctacctc gtagcttgaa aatcgagatc gagtgttaat accttccgct
     3001 acaccaagaa gttgagataa tttttcttga gataatccag cctccatcct cgcttctttc
     3061 agtcgtttag gcaccataat actacctccc tttttttggg aatcccatct tacagggcgt
     3121 tacaggcctc aaagtcaaca taatgaatca ggattgattt tgaatcgagt aatgttatta
     3181 tttctttaga ttcttgatat tgacacacaa gagtagctgt ataaaatcac aactgtgtat
     3241 ttttacagtt aacacataat gagtacgccg tcctaagccc ggcaacttag gacggcggaa
     3301 aatgggggct tgtgtccgta aggtgacgct tatgaataca tggtatcttt tatattgtaa
     3361 taacaacgat ttagaccgca ttgcatatcg ttgtaaaact atgaacgttg tggctttctg
     3421 tccgagatac ataaagatca cacctcggaa agattgcaac gcagtacgca aggaagaaaa
     3481 agttttattt cctaactacc tcttcctcca ttttgatgtg aatgtcacgc acacatcagc
     3541 aataacctct attccaggtg caattggctt cgtaagtttc ggttccacaa tatgtactgt
     3601 accggaaaaa gtcgttatcg ctattaaatg cgccaagata ctggcgctaa atcctgatga
     3661 tgattccata gagtgccgta acatcggtag tgacctactg gcagaaattc aacgcatatc
     3721 ttcaatcatc tcaccgttag aacgtcaaac ggccttctcc aggcttttac aaaatcagta
     3781 aactagacct tcattccttc tccagtcgtg ccgctgtgct tttacacacg gatatttcat
     3841 acaggagcta taaactcatg ccgaacccta taggccaggc taaatctaca gccgttattt
     3901 acatcggcaa aaaacgctat gaaacactgg caaaacacgc cagagatata agttatgttg
     3961 cggaggtaaa tattcgtcca agtgcctttt tgcagtacat gatcgacaaa ttaagtaatg
     4021 aagctcacaa cgaaatgttg aaagagctaa tgtcaaaaga ggataaataa atgcccaaac
     4081 ccacctataa cgtatacgtc acacaggaag ttcccactga cgaaaacggc ggtaaaaata
     4141 cctactggac caaggttggc gcagcattcg cgcataacgg aaaaccagga ctgaatatta
     4201 tgctcgtccc tggtattgct gtttccggca agctggtact actcgaacct aaagaagacg
     4261 ccccacaagg aaactaattt agcgagggta ctatgctttc caccgtttca tttatggcgg
     4321 tggctatgca gtgtgccgcc acagttcacc cctccacatc actggatgtt gcgcgggtcg
     4381 agtctggttt taatccctac gctattgctg agatcatccc caaacgtgaa cgccagccag
     4441 gcgacaaggg ctttatctca catatgccaa aaactaagga ggatgcatta agcatcgtca
     4501 aacagataga ggcaaaaggc cgtcgctatt ctgttggtct gatgcaaatc acaagtacaa
     4561 attttaatag ttacgccgta acggctgctg acctgttcaa cccgtgcaca aatctttctg
     4621 tttttgaaaa gatcatcact gattgctacc agcgaggcgg cacactgaaa cgggcgctga
     4681 gttgttatta ctctggaaat tttactacag gacaacaacc agaagctgcg ttatccagaa
     4741 ccagttacat acagcgaatt ggttactcac ctgaaaagcc ccgctatgtc gttcctggaa
     4801 ccagggatga tatcgcaact caatcggcca ttctgaatgc aacacccgtt gaagctccgg
     4861 cacgccctcg cgttgtctgg cctggcgcaa tcgtgcgcgg ggttcctgct caactgcgcc
     4921 agaaaaaggc agataccgtt tattaccccg cacaagttat acgcggaaac agattttcaa
     4981 ctgaaaaaga ggattaaccg atgtttaaca aattaaaggc catttgcagc cgcgcatctg
     5041 cttatattct ggctccgtta gtaagtgttg tcagtccagt tttcgcggct gacggtttca
     5101 gccgagcaaa taccatcatg gaaaaggttt cgaagggtct gcatggtctg gcagccgtca
     5161 ccatcaccgt cgctgtgatc tggattggct ataagactct gtggaaaggc gaaagcctgt
     5221 cgcagtgcgg ttacatcatc attggcggca ttttaattgg cggaggcagt gaaattggcg
     5281 ctctgctaat gaactaagag gccgaacata tggcaaagct gcttaaggcg atgaaacgtc
     5341 cggcggcgct gtggggggtg ccaatggtgc ccctgctgac tgtcgtgggc gtgacaatta
     5401 ttgttgctat ctggacttcc atagccctac ttctcttcct ccctattgag tttctggtta
     5461 tgaaatcact aaccagaaat gaacctctga gatttaacct gatagctgtc tggctaaggg
     5521 taaggggtaa tccggtagca aaccgtttat ttggcgcaac cgcatttatg cctgttgaac
     5581 ataatgctgt tgatatccag gagtttatcg atgcgatgaa gctaaaccaa tgtgccacca
     5641 taaaaaaata cattccttac tcgtcacata ttcaccctaa tgttgtccgt tcactgaata
     5701 gcgatttttt ctgtacctgg gaactgacag gaacgccgtt tgattgtgaa tctgacgaga
     5761 cactacagtt cggtacaaac cagttgcacg ggttaatacg ctcatttgaa ggctttccgg
     5821 ttacatttta tgtacataac agtcgtgaac agtttacgga ctcactgcat aaggtttcag
     5881 gaaaccctta cgcggatacc gtgagcgaac gctattacgc atcactgaat aaaaggccat
     5941 tccgacgtaa ccgcctgttt gtgaccatct gctatcgtcc gtttgccagt ctggataagg
     6001 caaaccgtaa acgtatgaaa gacggtcaga agctaaaaga gatagacagc gcattacttg
     6061 aagtgctgga aatcaaaagt acgctggata cagccttatc ccgttatggt gctcacgcgc
     6121 tcggtacttt caccgaaaac gaggctgtat tctcgtccca actggcattt tatgaatacc
     6181 ttctcacgca tcaatggcga aaagtcagag ttacacgtac ccccgcttac aacgtaatgg
     6241 gaaatgttgc gctgtacttc tccgccgaat cagggcaaat aaatcatgtt aatggcactc
     6301 aatatttccg tggtctggag gttaaagagt tttcggaaga aaccgcaacg ggcatgacag
     6361 actccttact gtacgcgccg tgcgattatg tgattacaca atcctatacc tgcatgtccc
     6421 gtgaggaagc gaaaaaggcg attaagcgca cccgacggct gatgatgagt gctgatgatg
     6481 atgctgtatc tcaacgtctg gatctcgatg tggcacttga cctgctcacc tccggtaaaa
     6541 tcgcctacgg taaacatcat ttatcaatta tggtgttttc gccgtcactg gaaagcctga
     6601 ttgcagacac aaacgagatc agcaatgcac ttaacaatat cgggataacc cctgttcctg
     6661 ctgaaatctc attgtctgcg gcctatatgg ctcaattacc aggcaattac aatctgcggc
     6721 cacgagccgg agagctgagt agcctgaact ttgttgaact ggcatcactt cataatttct
     6781 atccaggaaa acgcgacaaa gccccgtggg gtgacgcgat ggcactggtg cgaaccccgt
     6841 caggtgatgg ctactatctg aatctgcata acactctggc agataaagac gagttcaacg
     6901 aaaaaaaccc tgcaagtacc tgcattctgg gtacaaacgg ttcaggtaaa acaatgctga
     6961 tgacgttttt acaaatcaat cagcaaaaat ataaccgtga tgacagcttt tcaccggatg
     7021 ccaggactaa acgactaacc acagtttacc tcgataaaga ccgtggtgct gaaatcaata
     7081 ttcgcgcact tggaggaaag tactacaggg tattcagcgg tgagccaaca ggctggaacc
     7141 cgtttgctct gccagccact aagcgaaaca tcaatttcat taagcaactg atgaaaatcc
     7201 tttgtacccg caacggtgca accataacgc cacgtgatga acgtcgcctc aatgatgctg
     7261 taaatgccgt catgagtgat gaacctcaat atcgcgtcta cggcataacc cgaatgctgg
     7321 agaacctacc ggaaccggca acgcgtgaag cgcaagaaaa cggcctgagt atccgtcttt
     7381 cacagtgggc gcgcggtggt gaatttggtt gggtgtttga taacgaacac gacactttcg
     7441 atatcagcaa ctgcgataat ttcggaattg acggaactga atttctggat gacccgaatg
     7501 tctgcgctcc tatctcgttt tatctgctgt accggataac cagcctgctg gatggcagac
     7561 gacttgttat tttcatggat gaattctgga agtggttacg cgaccctgta tttaaggatt
     7621 ttgcgtataa caagctgaaa actatccgaa aactgaacgg tatgctggtt gttggcacgc
     7681 aatctccagc ggaaataatc aaggatgaga tagctccggc agttattgag caatgtggca
     7741 ctcaaatact ggccgccaac ccaagcgcag atcgctctca ttacgtggat ggaatgaagt
     7801 ttgaaccaga agtgtttgat gtcattaaag gacttgatcc acaagcgcgt cagtatgtcg
     7861 ttgtcaaaaa tcagttccgc cgtggagata caaaacgctt tgcagcccgt atcacgctgg
     7921 atctgtctgg tatcggcaga tacacaaaag taatgagcgg cgacgcacct aacctggaaa
     7981 tattcgaatc actctatcag gagggaatga agcccagcga ctggctggat tcctacctgg
     8041 ctaaagcact ataaccaagg agatatcgat gaaaagattt cgccccctta ttctggcggt
     8101 tcttattgcc agtccgatag taaacgctgc gggtataccc gtatttgatg caacagccag
     8161 actggagaat attcgccagt ggacaaaaga agccaatcaa tggctacaga cggtacaaca
     8221 ctataaagac cagctacagg cgtatcaaaa acaactggaa acagttacag gcatccgtga
     8281 cattcaggcg ttcgttaatc aggccaaaag ccttaaagct gacctggata aactacgtaa
     8341 gccagggcaa gcactgaacg atttgctctt atccggcggt tcttctggcc aatttgatga
     8401 actgtacaac aaatacaaga ttttcgataa ctgcaatgct gaacaatcaa aaagttacgc
     8461 caatacatgc agacaagagg tcatcaacaa agcaatacaa atcgaacaaa ccaatgaagt
     8521 acaaacacag attggtgaaa tgcttggtga gataaacgaa ttatcagacc gcgtagcaaa
     8581 tgctaaagat atgaaagagg cccaagactt agcaaacagt attcagttaa aatctgtgat
     8641 gctaaatacg ctcacgaccc aatgggaaat gagtgtgaaa gcagcagaaa agcgcgaaaa
     8701 agtgcttgag gcggagcgtg ttaaacagtg gaatcagcag cagttaaccg caccaacagt
     8761 tgatttaaat agctaaaggg aaatacaaaa tgcgtttatt aacaatatat cttttggcat
     8821 tatcagtttt cttaattagc ggatgtaaag aagaggctaa aacaaccaaa tggtataaag
     8881 accatccaga agaactaaaa gctgtttatg aaaaatgcca gaaaacagga agcgcttcgg
     8941 aaaattgtaa aaatgcaaac gaagctcact ggcaaataca gcagttgaat gctccgaccg
     9001 tcgatttaaa ttcatagtga ggtaaatatg tccggtggtg tttttgtcgg tgttgaaaaa
     9061 tacctgatag gaggaataac agacgccaca aagggattaa tgatgtcgta ctcttctatg
     9121 atgatggggc tggcagcagc atcagccacc atctacatca tgtggcgggg ataccagact
     9181 ctggctggta agctctctac gccgatggaa gacacgatgt gggacatcat gaggatggcg
     9241 atcatcctgt cctttgtggc taatttgggt ggttatctcg atggcgttat cgacgctgtt
     9301 aatgggttaa aggaggggtt ttctggcagt gataatatat ggcagctatt agatagctta
     9361 tggaataacg caaaagtatt aggtaaaaca ttacatgacc tagataattc caaatatata
     9421 aaagatgagg gaatgaccgc tcaattttat gtatggcttg ggatattcat cttaatgatt
     9481 attaccgcat ttgtttctat gatagcggaa gtcatgatct tattgctgag tatcactgca
     9541 cctatattta tattttgctt aatgtatggt tttctacgcc ccatgtttaa taactggcta
     9601 caaaatatat ttgcagccat attaactatt ctcctttcct ccttatcatt aaggattgta
     9661 gtcaattatt taaactcaag gcttgcatta gctactcaag gtgcagccca agcaaatatt
     9721 gtagaactag gagcacaagt ttgtcttgct ggagtatgcg ctgcaatact ggtgtacata
     9781 tccgcaaaac tagctggggc acttgcagga gcaagtgcta cagccactat gcaaggtgcg
     9841 gcggctctcg gcattggcgc tgctgcatac ggcgctggaa aaatgttaaa ggaggcagga
     9901 aaagtattgc ctaaagaagt agcaagtcca agtaataaag ggttaggaga tccacaatca
     9961 cgaatagtat ttaacgctgc tgatgcacgt aaacacgcta tagaacaaat gatagcgcgt
    10021 aatgaagcac ggatgctgac agatcaatca acaccaaaac taccgccatc aattaaatgt
    10081 ttaccccctc caagataact ataaaaatat aattatattt ttatagaaaa ccaaaaggta
    10141 cataaaatga aacgcttaat cgcgctggct ttgctgtgcg tgctttctgg ctgcgcgcaa
    10201 aaatctaaat ctctgccgcc cgcattcagc ggccagggta gccctgtaaa cacccctcaa
    10261 attatcgcgg agctaaccac acatgactga acagaaatta attgctgatt cccgctcgtt
    10321 tgagcagcaa atgattgaca gggataaacg cgccacaaag gcaggttttc tggtcggcgg
    10381 agcagggtta ttaatcgcta tcctgtcact gatcgctata gtgattatgt tgccacttaa
    10441 acaaacaact gttgaactct ataccgtaga taatcatacc ggacgagtgg aacacgttac
    10501 ccgtgcatca aaaacagccc tgacagcgga agaagcctac caaaaagcta tggccgccaa
    10561 ttacgttaaa ttacgtgaac ggtacgttta tcctgcatta caggacgatt acgagacagt
    10621 acagatctac aactcaccac aggtaaacga cgactacctg gcactgtacg ccggaaaaaa
    10681 cgcaccagac aaggtttata ataacgcggc tcacaccgtg aaaattgaaa ttatttctga
    10741 gcagataacg aatgcaacag caccagacaa agtagccacc gttcgttata aaaaaataat
    10801 tcgtcgcatg gctgataaca gtattcgcaa tgaatactgg gatgcacgtt tcaccttcca
    10861 ttcagaccct gaaaaagaaa tgtcagatgt tgagcgggaa ataaacccgt tcggattcac
    10921 cgtaacaagc tggcagactg accgcgaaat caggggaggc gagtaatgag aaaactgacg
    10981 ttaattaccc tgataataat gctgaacagc caggcatggg ccgcagctac accacaggcg
    11041 agccgttatg atgcacgtat ccagcaggtc atttataacc cgcagaatgt taccgttgta
    11101 aacacaaaac caggatttat gacgactctg gtttttgata acgatgaagc tgttatcagt
    11161 gccagtcctg gctttgatga agcatgggaa gccagagcag atgcaaacag ggttaacatt
    11221 cgtcctgttg cgctcacaca gggcgcacca ggtgaagatg gtaatacaac acaggttgtt
    11281 atcccgccga acagccgtga ctggcatacc aatatgctga ttgtgacaaa taagcgcctg
    11341 tacaacgttg agctgaatgt cattgatgaa aaatcgccac aacagcccgc atacctggtg
    11401 agttaccgct atccaggaga cgaacgtaac agagctaata aggaagcagc agcgcgtcag
    11461 aaagaatggg aacaaaagca ggaacaaacc cagattcaga aagcactgag aaccgcacaa
    11521 accccgcgta actggaacta cacgaaatat ccaggaaaag acagttacag cattgaacct
    11581 gattttgcct acgacgacgg tcgctttacg tttatcggat tctcacccaa caaatcagtt
    11641 ccatcagtta ccaaagaaca gaacggccag gaatacgtgg ttaacagcag tatacagcgt
    11701 aagggcaatt ttaccgttct cgttatccag gaaatcacac cacggcttgt attacgttcc
    11761 ggtgatgccg tagtggggct gaaaaataac ggctttggaa aagtacacgc ggctgataat
    11821 tcaaccgtat ctgacaaggt ggagcgcgtt gaaaaaacag ataacaccta atctataaaa
    11881 atataattat atttttatat aaggactcat atatgactga cgaaaataaa tccccctcta
    11941 cggaagaaga aaagccagtg acgacatctg atcaggatga tatcgccacg ctggaacgag
    12001 aagccagagc aaaacgtgag gctgaattac aagcatcaca ggacgatgaa aaggagaact
    12061 cattacaacc agcagtaaac aaactgaaaa aacgtcagaa ggggaaagca gcggcctttc
    12121 tggcaatatg cgcagtagcc ctgattggcc tgacatgggg cggtaactgg gtatatcaaa
    12181 acttcctgaa gcaagaagat ggagagaaaa caaaggaggt tacaccacct aaaaaaacga
    12241 atgactaccg gaaacgaacc gatttaggca tggccaccaa tgagccggaa aattcgactg
    12301 agggagagca aactgttaac caggataaca cgggcaatgt agccggtact gccggacaga
    12361 tgaaaacatt tgagccacca caactgaata aggccagttt ccttgttcgt cgtaataata
    12421 cggccagccc ccaaaatcag gtcaaaacaa gacaacagga aatgatactg gctgcggaaa
    12481 acaacgccac acagcctaca gacagtacag caaccacaac acaacatggc actgcatcac
    12541 aggcaatttc ccccgcgaca gtgcgaagaa tcccctacaa ccctgatttg tatataccgg
    12601 aaaacacagt cattccatgt tctatggatt accgctttgt ttccgatcgc gctggaagaa
    12661 tacgatgtac ggtagctaaa gatatctgga gtgccagcgg caatacaaaa cttatcgaga
    12721 agggaaccac ggcaacagga atataccaga cgggaattac acaagggcaa gggcgcgctt
    12781 ttatcatgat gacaaaatta cgtacccgac agcgaccata tctggatatt ccgctgattg
    12841 ataccaacgc cgctggcgag cttggagaat caggtgttga tggatggatt gatacccact
    12901 tctgggagag gtttggcggc gcattaatgg tcggcatgat cccagatatt ggcgcatggg
    12961 catcaaacaa tgcgggtaaa aaagaccgca ataccgacta tacagagaac agccgccagg
    13021 ctatggcaga tatggcgcgt accacgctgg aaaacagcat taatattcca ccaacgcttt
    13081 acaagaatca gggagaaata atcaacctaa ttacagggca ggatattgac ttctcaaata
    13141 tctacacgct gaggatgaaa tagaacatga cggttaaaaa catatctctt gatcgctata
    13201 aacaacgttt ttttagtgaa tacctggaat taccaggact gacagaaata gctgttaatc
    13261 gtccaggtga actctggaca aagatagatg gtgtctgggg gcaacatacc gttgatctga
    13321 aatttgacga ttgctataac tttgcacggt gtcttgcaaa acaccataac gataatatcg
    13381 aggatatcaa accaggactt tctgcaacac tggaatcagg agaacgatgt caggttgtcg
    13441 tacctccggc gtgcgaacgt gacacgatct caatcacgat ccgtaaaccg tcaaaaattc
    13501 aaattccgca tcaggcatat attgatagcg gtttttataa ccgcgtcaca ggcattgaaa
    13561 aagcatccac acataacgaa gaattgattg aattatacaa atcaaaaaac attcctttgt
    13621 ttatggaaaa atgtgtggag tatggaaaaa cattagccgt cgcaggtgaa accagtacgg
    13681 gtaaaacaac atacatgaaa atgttgatac aatatatacc cgtccattta cgcataacga
    13741 cgatagaaga caacccagag ataatattct acaaacataa gaactatgtt catctgtttt
    13801 atccttctga gtcatccgat gaaaaaggtt ctgttgtaac accagcggcg cttatacgct
    13861 ggaattaccg gatgaatcca gacagaattt tattaacaga agtgaggggt ggagaggcat
    13921 gggattttct gaaaataacg gggtcaggcc atgaaggaag tatgacctcc attcatgctg
    13981 gttcagccca tgaagcaatc gacggtttta ttacccgctg tactgaaaac ccacagtgtg
    14041 cgcaattacc atattcattc ttgctacgta aagttctcga ctctctggat gttgttgtga
    14101 gcattgattt agatggaaac gtacgccgta tgaatgatat ttatttccga ccgttacacc
    14161 gcaatcaata ttacgaggaa atgaaagtat gataaagcat atactggtta ctgtgcttgt
    14221 aacactaatt accggatgtt cctcaccgcc tccaccagta cccgtggaat ggaataaaaa
    14281 gccggtatcg cttaacgaga aacttcctga atggaacccc aatagcttaa taataaaatc
    14341 gcctgttatt aatgacaaat ggacgttatc cattagctca caagatttca ataaagaaga
    14401 ttggatgcca tctgtctttt atgccattgc tcactcaaca caaatcacag tagcaacaaa
    14461 gtcaggcgct gattatttca gtgcaaaaaa ctggttgtat catcatggtg caaacggggt
    14521 aattatatat caccccatca caaattgtct tacgtgcaat gaaacaacca tctacttttc
    14581 gcgttaaaac aaaatattaa acaaagggtt ctgacatgaa aaaaattgca gcaataacaa
    14641 tgtttgtttt aacaaccgcc acacctgttt actcttatgc cgcagaccca tgcgaggttg
    14701 ttttatgtat gtacggaaaa gctacaggaa acagcggagg tggcgaatgc agatcagcag
    14761 aaaaagcatt cttcaacatt cttaagaaaa agaaaggttc aatacgatgg agtaaaacat
    14821 ttgatgcacg taaagcattt ttaaatcagt gttcatccgc tgatcccgca gctatatcaa
    14881 aaataatgag taagtttggt agagttcgag ggtaaatatg aatacagatg aaaacatggt
    14941 attttatctg acaaactccg atcttgttta tagctatgca cacaagatag tgtgctccat
    15001 tccatcagga atgaaaggga atttcattac ttctatcggc gaaacgcttg aagatttaaa
    15061 gaaacgatat cctgatatta ttctcattac agaatcggaa gcattagcat tgcaatcgaa
    15121 gaaggaaaat taacatattc tttcagatct ggagaatcct atgttaaaag aagatatgtt
    15181 cattaataat attcattcaa ggaatcagga cagaatccat gtaaaaagag tctttgatga
    15241 tattagtaat aaagcaaaca ggggatgcgg gctatattat gaaatatatc aagcgaggtt
    15301 aatgcgaggg ttaattgagc acacagtaaa actgaaaagt tctgatgcca ataagctgat
    15361 tagttacgct gcttcacaag gttatgaaat aacagaagac agatataatg ctgcaataaa
    15421 agccgaggat gaatgtctga ctgaaataac acgggcacag gtctgaggca gaggattgcg
    15481 tagcaacccg aagggcgggg caaattcgct ttgcgaattt gggcgagtcc gacgcagggc
    15541 gtggtgcagt tcaggctttc tttatttctg gcttcaattt cgaagaaatt gttaagacag
    15601 gaataaagaa agtggtgcgt ggacagaaaa ttcaatttcg aagaaattgt taattttcag
    15661 gcaccacgcc cacccgcgaa cattcgcccc aagcgaattg aagcgggccg gggggagccg
    15721 caaaaatttt tccgccccag cgagaaaaaa ggcacgccca cgtgccggtt tttgtggggg
    15781 gcttgccccg cacaccgtcg ggcctaggcg cgacccccgt ctttaaagat gaattcccat
    15841 tttttcaagt ttacgcagaa aaaataggct aaattcatcg tcttaaaggc gggggtcgcg
    15901 cgtagcgcgc gacggtgtgc cgcctttgac cttgggggtt ttggtccggc gtccagacgg
    15961 gccattaccc ccatgtgacg tgaaggactg gcgttccagc caggccgaaa cggcacacgc
    16021 cgttgcccgt tcttcgggct ttcaggagcc agacgataac ttatcggctg gattctgaaa
    16081 gaacggataa cgggcatgat cgccttaggc gatcacaacg tttttggctt ttattggggt
    16141 acaataatgg ggtacagcta tggggtacat tatatccccc attactgtac cccattgtcg
    16201 taccccatag attcacttta tgaatgggga aaataaatat gaatccacat ctaatgcaaa
    16261 agctaaatgg tattgctgat gctttttcaa tatttctgtt atggctacag aacagccctg
    16321 tcatattgtc attactggca ggactaacac ttccatttat attcaaccta ccacgagagg
    16381 aaagaaaaaa cgctcccttc tggttaaaat ctgttgcgtg cgtatccatt ttctttttca
    16441 tatttggaac aatgtcacca cttagtattc atggactgag ttatctcttt gacgttctcg
    16501 atccaaatac actacttaga ataccattat ggataatcac tattctcttt acactagccg
    16561 gattagcttt tcatttgaca gcgagacgat tattagcagg tgaaatagac agtctgaaac
    16621 acagaatgat aaagaaaagc aaacttgaga gaaatacaag aacagatgtt cgcaaagtaa
    16681 aagaattgtt gccagacagc atagaataca atcccctaga ttacattgat ttgaaaaaag
    16741 gggttttcat tggcttagac aaagacgacc aaccgcaata tataacaata aaagaatttc
    16801 agaaacaaca tgcagcaatc ataggtacaa caggttcagg taagggtatt accgcatcgg
    16861 ttttgcttta tcaggcaatt ctgttaggcg aggctgtttt tgttgaagat ccaaaagatg
    16921 atgaatgggc tgtgcatgtt cttagagaag catgtaaaaa agcaggaaag aaatttgttg
    16981 taattaattt aaacagaacc aaccatcaac ttgacttact ggctgacata tcacatgaac
    17041 aattagaaga attatttaac gcaggattta gtctatcaaa aaaaggagag gccgcagatt
    17101 tttatcgtat agctgatcgc cgcgcagcca gaaatgtagc cgcaattcat aaaacaggaa
    17161 tgacactgta tgatttattc aacgatgaca acgtaaaatc aataggagaa gatgccccag
    17221 catttttagg tgaattagaa gaaatcgctc ttgtaaattc cgtcaatgca aaaaatggtt
    17281 tttctcttaa agagattttt gataatggag gatgctgcta tatcattgga tcaacccgaa
    17341 atcaaaaaat tatttctgca caacgcatga tacttacgcg tttgattcag atagctgaaa
    17401 ccagagacag aataaacaca acaccacgtg ttgttgcaat tttccttgat gagttgaaat
    17461 atcatatttc acgcccaaca atggaaggat taggtacagc acgagataaa agcgtgcata
    17521 tatttatggc cttccaagca atagatgatt taagagattg tccttctgat cttaatggcg
    17581 attctgtaat tggtgctgtt attgaaaacg caaaattcaa gttaatctat aaaatccaga
    17641 atccagaaac agcagagtgg gcagctaaaa tgactggttc catattagtt gacgatgaaa
    17701 tgaggaaggt gagaaccgac ttatcattaa cagaaaaagt tgatactgac cgcatgataa
    17761 gacaggcaga aagttattat gtagacagta atatgttttt aaatctacct gaaagagtag
    17821 gatttgtgtt cacatcaaaa gatttaccaa aagcaactaa aatgtctcat ataccagcaa
    17881 acaaagaaac cattgaattg ctatcttttg agacagaaca aatagaacaa cacaataaca
    17941 acgatatgcc ttccagtggt ttatgaggtt ttactatatg ttaattcatg attttcacga
    18001 aagaaaaaga atatcagcta acaacatgct ttgtctttta aatttcctta aggatgaaac
    18061 ctattcagac tttcacatta tcaaaagaaa attaggattc acaaaaaaaa gcagaaaccc
    18121 agtatataaa ttattaaaca aggctcttaa agaaggtttt attatcaaac atgaaatacc
    18181 atcatcaaaa agaaaattaa ctgtttgggg tattactatg caaggattag caatggttgt
    18241 aaaagccgat gacattacgt tccctagtta ttttgaaccc agcaaactaa aatactggac
    18301 tcttgaacat cgtcttttaa atcagcgagt gagaatatct ctggaaagta aaggagctac
    18361 tgaatggatt aacggtgata gtgcagcctt catcgcacgt tattccggct gctcccatcg
    18421 cccggatgga ataataaaac aaccaactgg cgcaatcatg gctattgaaa cagaacgaac
    18481 aatgaaaact cgtgctcgct atataagcat tataaacagt catcttacgg caagagataa
    18541 aggaaactgg cattatgctt tgtacgtcat gcctgacacc aaaagtaaag aatcattaat
    18601 cagactattc gactcaatca aaactgtaat gaggaataat acacctgtgc cttttgatga
    18661 aaaaaacaga gaaatgttta tttttagaac aattgatgaa ctggaacaat agttgatgaa
    18721 aatattaatc atattcgcac tatatatctt atcgtcttca ctatatgcca atactgcgga
    18781 tatacaggga aaagttactc gtgtacttga cggcgatact atcgagattt tacagaataa
    18841 aacgccagta aggattcgtc tggccaatat tgatgcaccg gaaaaaaaac aggcttacgg
    18901 acgctggtca actgaccaac ttaaaagcct gattgccgaa caatccgtca gagtgacata
    18961 ttcacacaac gaccgttacg gccgcattat cggacgtgta ttcacaacag gcggtagtga
    19021 ggctaaccgc tttatggtgc aatcgggtgc tgcatgggtg tatgagcatt acaacacaga
    19081 taacgatcta gcaactctac aacaggaagc tcgctcacaa aaacgaggtt tatgggctga
    19141 ccccaatccc gttccaccgt gggaatggcg acataaatag gagattgaca tgcaaacaat
    19201 taccacacgt gaacagttat tagtgaatgg gaaagtcctg gaaagggttg cgacacatat
    19261 cgtaactggc gctcatgggt atgaaacgct ttgtacaggt ggctataact tacagtacaa
    19321 ccaagagcga gtactggttc aaaattgtga aaagctggct gaaggcgaac tgcccatcac
    19381 ctgccacact tgtttcgctg tatggcagga tgcatacaaa ttcaaagata gcgactttga
    19441 cataaaatca ggaaatttca ctgatacagg tctgacagat atcacaattg gccagatttc
    19501 agaaccagaa gaagaaagtg tgaatattct ctatgcggat caatacgaaa cattgccaat
    19561 agaacgctat atgacaaatg aagaagtagg cagattagtg ttcaatttta tgaaacgcac
    19621 caacggcgaa gcgttactaa tagtgtttcc agaatatgat aatggagacg ataatgacga
    19681 taaaaatact cgttgtgtca aatgacggcc atgaaaaact aattgatcta agtccagata
    19741 atgatttagc taaaattaca aaatcattgc gtacagcaga aaacaggatg gtatgtgtta
    19801 tccaggacaa taatcgtatt ctccgctggg acagaagcta tgcctctcgt gccaaaaatc
    19861 actggcgaaa ggtagctccc gatcgttttg agattttagg aaccgttgaa catattcatt
    19921 atgttggtaa gtgttaatat caggggacag ttcgtcccct atgccatttt aatgatgctt
    19981 tttccagaat tcctggtatg cctccagcaa ctccccgtgc ttcatatcat tagccgctgc
    20041 aatcgagcgg aactcccgtt ttacttccat atccacataa aggttaagct gagcttttct
    20101 cccatcagcg tttttacgcg tcggattaaa tccgtgttgt gtatcctcca gacttggcgg
    20161 ctcgcctttg gtgttacgcg gttttggtgg tacattagcc atc
//
LOCUS       DAZOZD010000062        20148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOZD010000062 DAZOZD010000000
VERSION     DAZOZD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.062"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHUPB_001783"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHUPB_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR0758613.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHUPB_001784"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHUPB_001784"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR0758614.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHUPB_001785"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHUPB_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR0758615.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHUPB_001786"
     CDS             3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHUPB_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR0758616.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHUPB_001787"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHUPB_001787"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0758617.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHUPB_001788"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHUPB_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="ACHUPB_001789"
     CDS             complement(7691..8902)
                     /locus_tag="ACHUPB_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR0758618.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHUPB_001790"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHUPB_001790"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR0758619.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHUPB_001791"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHUPB_001791"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR0758620.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="ACHUPB_001792"
     CDS             11880..13658
                     /locus_tag="ACHUPB_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR0758621.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHUPB_001793"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHUPB_001793"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0758622.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHUPB_001794"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHUPB_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0758623.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHUPB_001795"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHUPB_001795"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR0758624.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /gene="actS"
                     /locus_tag="ACHUPB_001796"
     CDS             17775..18530
                     /gene="actS"
                     /locus_tag="ACHUPB_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0758625.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="ACHUPB_001797"
     tRNA            18609..18678
                     /locus_tag="ACHUPB_001797"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18883..>20148
                     /locus_tag="ACHUPB_001798"
     CDS             18883..>20148
                     /locus_tag="ACHUPB_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR0758626.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
BASE COUNT         4981 a         4765 c         5179 g         5223 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcattg ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaaga
//
LOCUS       DAZOZD010000063        19990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOZD010000063 DAZOZD010000000
VERSION     DAZOZD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.063"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            72..506
                     /locus_tag="ACHUPB_001799"
     CDS             72..506
                     /locus_tag="ACHUPB_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758627.1"
                     /translation="MKPAKIRLLEPQFSDYSGMLCGVKFENGVSVSELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYGERNDLNVDQIVEPSAPDIVPMKRGTADEPAKPIQTFTRE
                     ELESIADSEGIAGLRLIGNKIGVKAKGIVEMIDGIMKAQGGE"
     gene            506..1339
                     /locus_tag="ACHUPB_001800"
     CDS             506..1339
                     /locus_tag="ACHUPB_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758628.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNTFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDIVAAREDGILSESIGETTNMYRTGKSANVHVARSTWRLLIRYIDNRMIVRR
                     S"
     gene            1411..1755
                     /locus_tag="ACHUPB_001801"
     CDS             1411..1755
                     /locus_tag="ACHUPB_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758629.1"
                     /translation="MNISWQTEIAIYRFGTKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLESAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            1746..2219
                     /locus_tag="ACHUPB_001802"
     CDS             1746..2219
                     /locus_tag="ACHUPB_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758630.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIEGVKPRVGPHFLERAVEISEKQFSEAILEAAKKAGFTRG"
     gene            2221..2604
                     /locus_tag="ACHUPB_001803"
     CDS             2221..2604
                     /locus_tag="ACHUPB_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFR0758631.1"
                     /translation="MFIEAFANLMQKAKIGTVGTDIFCHYLPAYVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTIEKARKIIDMFPVEETESGEVYFRLVRPMAMP
                     VVYPKNDGSLIEAGIPVEFAGYLLN"
     gene            2678..3415
                     /locus_tag="ACHUPB_001804"
     CDS             2678..3415
                     /locus_tag="ACHUPB_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFR0758632.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QQGQTVISELVQGRNITITAPLAESVLKNMVDLMPGSTLSEDDNSVTITSAQGVNLIN
                     VAKELVLTPQDTTDYVLTLPKAATAGNFTMSYQSDDVRVFSVQFNAYPDDEGVLGKMS
                     GPKPVKNVTITPESPSVKVGATVQLTANFTPDDVVDKTGVWSSDNKKATVDHTGLVRG
                     VEQGSANISFTSNSGSKKVTKLVTVNQ"
     gene            3483..3800
                     /locus_tag="ACHUPB_001805"
     CDS             3483..3800
                     /locus_tag="ACHUPB_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758633.1"
                     /translation="MTKLLDLDSILPPKKSVKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LTNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            3926..4150
                     /locus_tag="ACHUPB_001806"
     CDS             3926..4150
                     /locus_tag="ACHUPB_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758634.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARAAQADGEGVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDRADPDAKEQLMQIFGRG"
     gene            4158..8714
                     /locus_tag="ACHUPB_001807"
     CDS             4158..8714
                     /locus_tag="ACHUPB_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0758635.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAVGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLINKPVRVRTSGSGNGGGSGGR
                     GGYSWHIGKNNEGSMFSGLRGNIFLLGEIGDAARTVTSIMFGWQKPIIEAAAEMERMR
                     VMLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLK
                     ALVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGI
                     TMGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFAD
                     RVGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQEL
                     VINLGKVVAAMVAFKLMRAGIMGVVGAGSKMVSTFATMATAIQTPFNLGATAVTRFNR
                     AARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMT
                     MLRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNS
                     AGVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAEN
                     QIFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSF
                     SSRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFER
                     DNAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFE
                     GKPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRS
                     ANASQRAAKKEQTAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLT
                     DLANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIIS
                     AGYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIK
                     ETGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDIT
                     NMVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGE
                     ATKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSM
                     QNLSSVTGALSAAFAMLGANASGSGNKWLSFGATVASGLVSAWAGGGFDSLTSSSAKT
                     ATSSVADGTKGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGSMNEAY
                     VPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMK
                     AIALETIAQEKRPGGSLNPNTQRN"
     gene            8757..9092
                     /locus_tag="ACHUPB_001808"
     CDS             8757..9092
                     /locus_tag="ACHUPB_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0758636.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            9175..9873
                     /locus_tag="ACHUPB_001809"
     CDS             9175..9873
                     /locus_tag="ACHUPB_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0758637.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            9866..10663
                     /locus_tag="ACHUPB_001810"
     CDS             9866..10663
                     /locus_tag="ACHUPB_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0758638.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLCRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            10651..11244
                     /locus_tag="ACHUPB_001811"
     CDS             10651..11244
                     /locus_tag="ACHUPB_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0758639.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVMMPALAPLGLSLMMGGIAQIISPQATNESVRQADNSNSYYFD
                     GPQNTTNQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            11261..15967
                     /locus_tag="ACHUPB_001812"
     CDS             11261..15967
                     /locus_tag="ACHUPB_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0758640.1"
                     /translation="MEQFKKKKLPLLIAGSGGKKSSKSSSRTPVEADDTVNSRAMASI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGISWDFRDGSQDQTPMSGFDFV
                     ETPKSINIQLKKSHYVTVSIDNDEADRVRVIMKFPSLRRIDQKTGDTNGTTVEYKFQI
                     SNGDSTFVDVVAEGEKNVGIKLTAKKTGAYYRSYELKLPKPGRAYSVRVVRITDDNSG
                     QYLYNDTWVDSIGEIVDTPMNYPNSALVGLKVNSEQFGGSMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGSFKLLSSSNPAWILFDLLTNSRYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVEFTKNSITLDAPVSLAKSGSFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETSGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THSGLGDSETWYYWVRSANKRGMLSRPNSNLGTEATTRDVLSFLANKITSSELGQALN
                     EEIDSKASQQAVDEINNHIDQSVESLEGEISALNKELTSSIDEFKTSFTERSETLENA
                     QSELRTEVSSTREKVDDALRQVENSNAALIELRNTVSKQGDSVAGVVEAANAALENAS
                     SLIAEERKVRAQADLANAKKIETMRSSVDESVAAVEEMRRTVAEVGRVSSEITTNLEA
                     ISKTNIDLALRQDEDQHRQLVNNAKIATTQKILVDDVSAMASKVEEIRAEIGDNIRAS
                     ILEEATARTDGDNALANRVTQLQSKFEGDISAAIRTEQSARASANETLSKQITQLESK
                     VNTDISTAIREEQQTRATQDEALSRQITQLQTKVNNDISAAIKSEQQARVSGDSALST
                     QINQLQAKVDDDISAAINEEREARASGDSALSREVSSLKTKTGKDIAAAVAVETKART
                     DANDALSRQISSLTTRANNLQSSIERESTSRTSQDSAIAKDLASFKTKTAKDISAAVA
                     VETQARAAADTALTSQINSLKTQTGKDIKAAVAEETKARTDADAALSTRVTNLQSQTT
                     NYINAAITSESKARTSADNALGKRIDTLKASVDGNVAVIQQQASAIADANRKVSTSYT
                     LKMETSTSGGQKYVAGIALGIDTTGLSQFLVRADRFGLVNNVNGKITTPFVIENSVAY
                     MNGAYIKDGTITNAKVGDLQSTNFVRGRAGWRFGKNGTLEINGNSGGNGRLVINGQRI
                     DVYDDNNVLRVRIGRL"
     gene            16062..19433
                     /locus_tag="ACHUPB_001813"
     CDS             16062..19433
                     /locus_tag="ACHUPB_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR0758641.1"
                     /translation="MWYREGTITFTQGSNTLVGAGTAWNVTANGVLPGMIVVAPDNKL
                     YEIKSVTNDTTLTLVENYTGETQSDIPCRIITTYEGDLTQFSARFTALLSRMSGDAKT
                     VRNWLTAVDEVTLEREDGTNVNVKPLSKIIAEHNKHVEWYENNTNAINSAGDKAKQAA
                     ASASAAAKSANLASSKATQASNSALDATASKNAANKSASSAKASETSALEAKNAAAIS
                     ASTASTKATEATSSANAALVSKEAAKLSEMNAASGAGSASTSANQARESARSAKTSAN
                     QASTYASAARASKDEASRKSAEASTSASNASSKSAESAAKAEEAKQSQTSALASAERA
                     EIAATRAERALSAISLHDASLTMKGIVKLSSETSSSSEEFAATPKAVKAAFDLAKSKY
                     TANDATTSQKGLVQLSSDTNNASESLAATTKAVKSVKDLADRKAAINSPAFTGTPTAP
                     TATQGTNNTQIATTAYVREAISGLVGSSPEALDTLNELARALGDDPDFATTMTNKLAT
                     KQPLDATLTALAGLTTAANKLPYFTGNDTAGLATITSVGRDILSKASVQAIIQYLGLK
                     ENGTSGDKIPLLSTANTWSAKQTFSNGLAGSLNGNSDTATKLKAARKIGGVPFDGSSD
                     INLPGVNSTGTQSTSGNAASATKLATPRSIGGMLFDGTANIDLPGVNKVGNQSTTGNA
                     GSATRLQTARTIGGVSFNGTANIDLPGVNKAGNQNTSGNAATATKLATARKINGVSFD
                     GSKNISITPKQLGAAKYVANLATGNTQEWTTAQFVAWLNSQGAFDATQWSTRCSWSYG
                     SNCYIGNASTGCGVIPLAGSVIEVFSTGTSNYTIRITTPSTSGGASGAVLNSEFIYTY
                     NGDNYSPTWRRNYSSQNKPTASEIGALASNGNAVSATKLKTARNIGGVSFDGTANIDL
                     PGVNKAGNQNTSGNAATATKLKTSRTIGGVSFDGSANINLPGVNTSGNQNTSGNAASA
                     TKLQTARTINGVSFDGTKNISISAAQIGALPIGGGTITGNLTVNGNGSFNDVQIRSDK
                     RNKRNLVKLDNALDRLEALTGYLYEIQLSDDSWKTSVGLIAQDAQKALSELVTEDTDV
                     ISGEKRLCLNYNGIIALLVEGFKTLRHEIKELREK"
     gene            19519..>19990
                     /locus_tag="ACHUPB_001814"
     CDS             19519..>19990
                     /locus_tag="ACHUPB_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758642.1"
                     /translation="MGVASGWVGSSAKSETGEQWMSAAGTKLGLGKPFMMSQMVGRSM
                     GCKIATAYYKGNPPDKVENWGAVGSDWPLAEKNQGTIAGASSCGLGRLVGIVLTQAHY
                     VPGLPPTGGLYIAGGRASNITVTVGGVSQVFTYNSVINGFHYYWQGTPSSAFITA"
BASE COUNT         5896 a         4129 c         5032 g         4933 t
ORIGIN      
        1 atgcttaagg gtgggctata cgcccaccct ttttgtaggt tttaaatatg cctgtatagc
       61 gagaaagcgt aatgaaacca gcaaaaattc gtttattgga accgcaattt tctgattatt
      121 cgggaatgtt gtgtggagtt aagtttgaga atggggtatc cgtttccgag ttacctttta
      181 tcgaccagca gaggatttgt gcatcaatgc gcgcatcaac tgttgagggt aaaaacgttt
      241 ctccatctgc tgcatatggc gaacgtaatg atctgaacgt agatcagatt gttgagccat
      301 cagcacctga cattgttcca atgaagcgcg gaacggcaga tgaaccagct aaaccaattc
      361 aaactttcac acgggaagaa ctggagtcaa ttgctgatag tgagggtatt gccggtttac
      421 gtttaattgg gaataagatt ggtgttaagg cgaaaggaat tgtcgaaatg attgacggca
      481 tcatgaaagc acaaggcggt gagtaatggc gcagatcgac tcgtatcgta gcggtgaagc
      541 tgtttctctt tcatttgcct tcaacgtgct ggatatcgaa tctgccacct acacagtaaa
      601 ggacagcacc ggggctattc tcgttgatgg cgagccgcta gaaattacca gcggtcaaat
      661 gtcgattccg gttgtcgtgt cggctgaata taaccagctc tctgagaaag aacgagatct
      721 gcggtacgtc attgtgaagg ctgttgcatc gggccttacg catgaagagc gtcaaatgta
      781 tgttctgctg aatacttttg aactgtcgat accagaacag tcgtttgcaa ctgtcgctga
      841 tgctcaaatg caggcgatcg atatgctgaa tggggacact ttgctgtcgg atggtgaagg
      901 cttaatgcgc aagcgtctca ttgaggctac aagacgtatt aaaactttac cgttctcaat
      961 ccgcaaaatt ctacgtatcg actttgaccg atacgatcgc cctcaaaata tgctgaatgt
     1021 gtatgacatt ccgtggggag cagatggagc gtaccggcat gatttagtcg attgggaaaa
     1081 gatgactcag gagaagtttg aagagttccc tgactacttc aaagaagcgt taatgctagc
     1141 cgttgttaat gaagcgtgtg agatagcaaa tggcaatgat atcgtcgcag cacgagagga
     1201 tggcattttg tcagaatcga tcggtgaaac gaccaacatg tatcgtacag gtaaatcagc
     1261 aaacgtgcat gttgctcgca gcacctggcg tttactgatc cgttatatag acaatcgtat
     1321 gattgttcga cgttcgtaat gtctttctca ttatttggtt ttggaagcaa tagttttggc
     1381 tggcaatgag atcgacaaca gggagagagc gtgaatattt catggcaaac agaaattgcg
     1441 atctaccgct ttggcacaaa gaacgtctac ggtgaagcgc aattgcagtt cgtcaggaag
     1501 acgaatgtcg gtgtagttaa attcgaacag agtaatgaga aatcgtcagt tcgtgctgat
     1561 agctctggta gtcgtggcaa ggcgagtctg gagctgtttg atgctgtgtt agttgtccct
     1621 ttggaatcgg ctgtacaact tgatgatgta ctgatccttg aaggtcaaaa attaaaggtg
     1681 tccagtgttc atcgccgctg ggggctacga ggaagacctg ggcatcttga agtaggggcg
     1741 aatatatggg tctgaaatac gatgcgcatc agttcaaacg tgcggggaat agactcaaca
     1801 acagccagaa agcatttaag cgatatctta ttcgggatat ggaaaagctg gcgcgtttgg
     1861 ttgagcgcct cagccgtgca atggccccgc tggaaaccgg atctctggag acagccatct
     1921 ttgctagggt gattaaagaa ggttattcag ggctgcgcat tgagctttct gtgtctggag
     1981 caaagccacg tcaggggcat cctggcgtag aagtaggtga ctatgctaag tacatggagc
     2041 tgggcaaata ccgtctcggt tatctttctc gaatgaagaa cgtgacaaac ccaccaattg
     2101 aaggtgtaaa acctcgtgtt ggtccacact ttctggaaag agcggtggaa attagcgaga
     2161 agcagttctc cgaagcgatt cttgaggctg ccaaaaaagc cgggtttacg agaggttaat
     2221 gtgtttattg aagcatttgc aaatttgatg cagaaggcaa agattggcac ggtagggaca
     2281 gacattttct gtcattactt acctgcctat gtcaaatccg gtgtcctgct tattaaccca
     2341 aataccggta tcagtatcga tcatgagtta cagggctttt atcatgagtc gttcacaatc
     2401 attgtgcgtg gctcgtcaat tacaacgact attgagaaag cgcgtaagat cattgatatg
     2461 tttcccgtag aggaaacgga atctggagag gtctattttc gacttgtacg gcctatggct
     2521 atgcctgtcg tttatccgaa aaatgatgga tcgttaatag aagccggtat tccagttgaa
     2581 tttgctggct atttattgaa ttaaataaat aggtaagtat atacttaaat atagcgcaat
     2641 gaatgcgtga aattaacgga aaaaggagtt tccatcaatg tctaataccc atgtaaaaaa
     2701 catcaagctt ggtgcctgca aggtatcatt tggtggtgtg gatctgggtt acaccaaagg
     2761 tggtgttcag gttgaagtag ccactgaaac gcttaaagtt acagtcgatc aacaaggtca
     2821 gacagtaatc tctgaattgg ttcaggggcg taacattacc attaccgcac ctctggccga
     2881 gtctgtgctc aaaaacatgg tcgatcttat gcctggttct actttgagcg aagatgacaa
     2941 ctccgtcacc atcacatctg cacagggcgt aaatctgatc aacgtggcga aggaactcgt
     3001 cctgacaccg caggacacca ccgactatgt gttgacgctc ccgaaagcgg caaccgctgg
     3061 caacttcact atgtcctacc agtctgatga tgtacgtgtg ttctctgttc agttcaacgc
     3121 gtaccctgat gatgaggggg ttcttggcaa aatgagtggc ccaaaaccgg tgaaaaacgt
     3181 gacgattaca cctgaatctc cgagtgtaaa ggtcggggcg acagttcaac tgactgctaa
     3241 tttcacccca gatgatgtag ttgataaaac aggcgtatgg tcatctgata ataaaaaagc
     3301 cactgttgat cacactggtc ttgtgcgtgg tgtggaacaa ggttctgcaa acatatcatt
     3361 caccagcaat agcggtagca agaaagtaac caaattagtg acggttaatc aataatcaag
     3421 caacttatta atctataaat tgaggctcat gatgggcctc tcttttaaaa ggattttaac
     3481 aaatgacaaa attactcgat ctcgactcca tcctacctcc taaaaaaagt gtcaaatttg
     3541 gcggccagga atatcccatc gttgaaatga cggtaggtct gttcgtttcc attaagcaga
     3601 tggaaggtaa agatctgacc aatatgtctc ctgttgagca agtgacagct tatgcagatc
     3661 tggttcgcaa ggttatccct tcagtgcccg acgaagttct tgaaaaactg actgttccgc
     3721 aacttcagca gatcttcacc ttcgctatgg aagtgattga tgaagaaaac gaaaaagcgg
     3781 ctggcgaagg ggcaaagtaa tttcccgcga tgaatccggg acaaggaccg tctcaataga
     3841 tctcggattc tatttcagtc gtatagttgc tcactacgcc gtgtcgccgt tagaactact
     3901 gaacatccct ctcacgatgt tctggatgct cagtcgcaac atcgaccgtc tacgtgcgga
     3961 agaggatgtc cgcaacctgc aagtcgctcg cgctgctcag gcagatggcg agggcgtgaa
     4021 ggcgttcatg gagggtttgc aactcaggat tggaagacca gtcgtaacag ataaagtcta
     4081 cgatccaagc caggataggg cagaccctga cgccaaagag caactgatgc aaatatttgg
     4141 cagaggatga caagggaatg tcacaaaacg tagagtttat cctgtcgctg gaagacaaac
     4201 agtttacagc gtcaatcgat cgtgcgggaa aactgcttac tcgatttggt gagcaggtaa
     4261 caaagcctgc tcaaaaaatc caaacctttg aacgctcttt gggttcggtc gcccgtatca
     4321 ttggcgttct ggaaagcaag ctcgattcta cggcagataa actacaggat gtagctgtcg
     4381 gttttgagct tgcttcagat gctacgcgta aaatgcgtgg caacatcacc agcctcaatt
     4441 ctggtcttaa agctctgatt gagcgcgtcg atacgacaac ttcttcagta gataaactca
     4501 ccgcgtcatt acgtaaagtg caatcagagc taaatgattt ctctgactgg gcgacctatg
     4561 caagcaaaag cgcaagccgt tttggtacgg aggtcaaaga agcctctgcg tccgtgagtg
     4621 gcatgaatac gcgccttaac accacgacga agcgactcag taattggggt gtgacaacaa
     4681 gccaggctgc cgagggacta aaaaaagttc gtgagcagat ggacgaagtt atcggacgtc
     4741 agcaactcat taacaagcct gtacgcgttc gcacatcagg aagcggaaat ggtgggggta
     4801 gcggtggacg tggtggttac tcttggcata tcggtaaaaa taacgaaggc agtatgttct
     4861 ctggccttcg tggcaacatt ttcctgcttg gtgagatcgg agatgctgca agaaccgtca
     4921 ccagtatcat gtttggttgg cagaagccta ttattgaagc tgcggccgaa atggaacgta
     4981 tgcgggtgat gcttcgaggg ttgaataagg agaagtccaa cccgggccag gctgccgctg
     5041 atgatatgaa gtacatcgta gacatggctc aaaatgcgcc gttcgcgatg caggcgttaa
     5101 cagactcctt cgtgaagttt cgttctgctg gcttagatcc aaccgatggt tctctgaagg
     5161 cgctggtgga ctccgttgct cgttttggtg gtgatagtga gctgttgaaa cgtgcggctg
     5221 tggccgttca gcagatgtcc ggtaagggcg ttgtgtcaat ggaagagcta cgtcagcaat
     5281 taggtgaagc cgttcctaac gcgatgcagg caatggcaga cgccgcaggc atcactatgg
     5341 gggaactgac taaagccgtt gccagcggta cggttgaggc aaaacaggcg ttgtctctga
     5401 tgtttgttgg tctgcgtgcg gagaatgaga acgcagcgaa agacatgatg caaacctaca
     5461 caggtgcgct ggcgcaactt cagacgtcat tcacgttatt tgctgatcgg gttggtcagg
     5521 ccggatatct ggattctcta tcgaagggga tgaaagaact ggcttcaatc atgaatagcg
     5581 ccgaagggat ttcgtttgcc aattctttag gttcggggtt aacgacggca atcgatgggt
     5641 tgcgtcagct tgctcaatgg ttagcaaaga accaagagct ggtaattaat ctcggtaagg
     5701 tcgtggccgc gatggttgcg ttcaaactga tgcgagcagg gatcatggga gtagttggcg
     5761 caggcagcaa gatggttagc acctttgcca cgatggcgac cgccatacag actccattta
     5821 acctcggcgc tacagcagta actcgattca atcgtgcggc acgtatgggg ctggctccga
     5881 tcccctctct tattttcgcc atccgtgggg cgattacggg gcttaaaggc gcttttgctg
     5941 gattaacggc gttcattgca gcaaatccta taggggcagc atttacagtt gccactgtag
     6001 ctgttgctgg cctaatcacg tacatgacca tgctccgcag cgaaacttca aaggtcgttg
     6061 acgagattag gaaaatacca gaggcgatga cggcggccaa acgtgcacaa atggccgacc
     6121 gcgcagaaaa gctcgaaaag cagattcttg ctgatcaacg agcattaaaa actggtgagg
     6181 gtgttaatta cgtatctaac tctgccggtg ttattactca caaagagtcg aaggctgaca
     6241 tcgaagcacg cctgaaaaaa aatcaggaag agtatcaaaa gataaccggc acgatagctc
     6301 ttggtgacgg cgctgtagca aagcgtttgg ctaaagaggc tgccgaatct cagattgaga
     6361 aaattcgagc ggaaaaccag attttcgcgg caacattcgt gaaagcccgg caggaggctc
     6421 tggataagat ccagaaaatc aatgatgacg gttcactttc agatgacgaa aagaacaagc
     6481 tattggcacc gttacgtgaa acggtaaaca aaagctatct ggagcctgcg caaaaactgg
     6541 ttgattcatt ttcttctcgt aagaatgcta ccgagaagca aatagcgact cttaatgatc
     6601 agcttgaaaa agccaaaaag gatggaaata ccgagcaaat ccagaaactg caaggcagta
     6661 tccgtggtta tcaggagcat ttggaagccg ttgctcagga actgacacag gcagagttcg
     6721 agagagataa cgcggccaaa actggtaagg gcgtaatgtc aaaccagggg actgttcttg
     6781 ggttaggtac aactgataaa gctgctcaga aggcgctggc gcaatatatg cgaaaccaga
     6841 tggattctgc gacttatcaa cgtactttgc ctgacggcac tccgatgatg gacttcgaag
     6901 gtaagccgat tattgggcct aaacaactca agacgcagct taatttgcag aaagcatcca
     6961 gtgccagctc tctggagaaa atgagcgatg aagagcgtgc cgcagccatt gccgcactga
     7021 ctaaagctcg tgaacaggat gccgcagccg ccgagaaagc agcccaacga tcagctaatg
     7081 cctcgcagcg tgcggccaag aaagaacaga cagcgcaaca gaaactggca gccggatacc
     7141 agaaggctct ggataaagcc gatcagctta tggggcaaat gggtgaaagc tctaaggcta
     7201 cggtatcgtt tgatcagtct cttcgcgata caacgaaatc gctgacagat ttggctaatg
     7261 cggttcctaa cgagttcatc actcaagaga tgatcgacaa ggcgaaaaaa cgtcttgaag
     7321 acctcaaaaa tgcgacacct gaatatcgcg agatgtttaa tcgccgcaat gttgagcaga
     7381 tgatctccac ctgggcaccg gagtcggatt ccattattag tgctggctat acgccgtctc
     7441 gtgaagagaa agttgctgat ttcgaagaca cctacaaccg caatctcaaa gcgttgatag
     7501 aacttcgtga taaggcgtct gatcctaaag ttgtggcgct ttatacaaag aaaatcaatc
     7561 aactgattgc tgctggcaat accgcgctta ttaaagagac ggggactgcg acgcagaagt
     7621 tggcactgga atacgaaaac ctggcagagc agatcgaaag cacctggact gatttgttta
     7681 gtggcttaac tgatgtcctg actgatttcg ttattaacgg gaagatgagt ttctccagcc
     7741 tatctcagtc cattttgaaa gatatcacca atatggtcgt gaagtcgcaa atcacgctgc
     7801 ctctaatgaa catgttgggg atgggaacca ccgcagctgg tagttcacag agtggtaatt
     7861 tgctgaccgg ggttgcttcc gccgttgcca atcaaggggt acgaatgggc aacactgtta
     7921 acggcgacaa gtcggtagga gaagccacga aggagacgtc cagttcggta actggattag
     7981 ggcaaacaac acagcagacc accagcgcaa ttggcactgc aacaaatgcg attggtagct
     8041 gggtatcagg gctatttgat agcactgaag ccaaagatgc cgagacaaaa gccgtgaagg
     8101 actccatctt ctcgatgcag aacctcagct ctgttaccgg cgcgctgtct gccgcgtttg
     8161 caatgcttgg agctaatgct tccggctctg gtaataagtg gttgagtttt ggagcaacgg
     8221 ttgcatctgg tcttgtttca gcttgggctg gcggtggttt cgacagcttg acatcaagct
     8281 ctgctaaaac cgcaaccagc agtgtggctg acggaactaa aggcattcct gcaatcccga
     8341 agtttgcaaa tggaggaata ttcggaaaag acggcgtgat cccgctccgg gcataccaga
     8401 aaggcggtat tgctaactcg cctcaattag cgttgtttgg ggaaggttct atgaatgagg
     8461 cgtatgttcc attgcctgat ggccgaacaa tccctgtaac gctcagtacc gatggtatga
     8521 gtggaggcgg aaatgttctt tctcctgtat caattgagat caacgtccat agtgacggta
     8581 gcacaactga atccggcgat acagaaagca tatggaacaa tgccgctcaa cggatgaaag
     8641 caatcgcgct tgagactatc gctcaagaga aacgccctgg cggatcactc aacccaaaca
     8701 ctcaacgtaa ctaactatcg actgccccgg tcggggcagt ctcacaagga tgtgagatgg
     8761 aaagacaaac gtttaattgg tatccagatt acgaatctga aaagagcgta aaaccgaatg
     8821 taacggtact taattttggt gatgactacg agcagcgaca ggctcaaggt cttaatcgta
     8881 ttaaagaaga atggtcgtta acctttacca gatcatacaa cgaaattaat gcaatcgatg
     8941 acttccttac tgagcgatca ggtgttgaat cgttctattg ggttaatcca agaggcaagc
     9001 agattgtagt tgtatgtgac agtcatacgg tcaagagata tcaggggtac tgtgtcttaa
     9061 ctgctacatt cagacaagta tttgaggctt aagtatctgg ataagtaagt actaatttac
     9121 tatcattgtg gcgctgacag gatgtcagcg cctacttatt tcaaggatga aacaatggga
     9181 attaaagctg atattcagag cttatctccc tctgcactca ttgagttgtt cgtactggat
     9241 atgtcgaaca caacttcagg ggggaagcta ttcttccacg ccggaacaaa cgaactgatg
     9301 caaccggtcg tctggcaagg agtgacatac gagccgtggc caatcaaagc atcaggcttt
     9361 gacaaaactg gccagggaac gttgccacgt ccaaaaattc aggtatcgaa ctttgccgga
     9421 accgtctctg cggaagttca ggcgaacgac gatcttgttg gctgccgcat tattcgcaag
     9481 atgacgctgg ctcgcttcct cgatgccgtt aattttaaag acggcaaccc aacagcagat
     9541 ccaaaccaac atttcccgga tgaaatgtgg tttatcgaac agaaaactct cgaaactcat
     9601 caggttgtcg agtttgaatt gtccagtgtg ttcgatttga tgggggtaca actgccgtat
     9661 cgtcagatca ttaaaaacac ctgcccgtgg aaataccgag ggccagaatg cggctatacc
     9721 ggtccatatt tcgacaaaaa taaccagcag acgtctatgt ctggtgcgga ttactgcaca
     9781 aaacgttatg acgcctgtaa tgcgcgtcgg aattattttg ccgacggtgt gatccatttt
     9841 ggtggattta ttggagctac gcggtatggg taataaagca atccctgagc ttggctctga
     9901 cgttatgcag caaatctatc tctgcgccat aaatcgctac cctaatgaag cgtgtggctt
     9961 tctggttaga actaatggcg acaaatatcg ctttatggaa gcgcggaatg tttcggagaa
    10021 cccgcagaac acttttgtaa tgcacgttga cgacattatg gcggctgagg atgcgggtga
    10081 tgttatcgca atctggcatt cacatactga tgaatcagca gaagcatctg atgccgatcg
    10141 tgcaggctgc gaagcgacgg aagttccgtg gatgattctg gctattcgca agaatgttga
    10201 gggagatgcc cctttccatt ttagcgagat gaatgtgatc acacctgatg gtttcgaaat
    10261 gccatacctg tgcagaccgt atgtatttgg cgtattcgat tgctggatgt tgtgtcggga
    10321 ctatttgaag cgtgagttta acgtcgaact aaacccgaac gcacacctgc atattccatc
    10381 gtggtacacc ggcgataacg acattctcga ccagaactac cgaaacgaag gattggtacg
    10441 gcttgcgccc ggaacagaac ctcaacgtgg ggacgtcttc tttatccaat acggaaaaat
    10501 gcctgaccac tgtgcggttt atatcggcga tggaatgatt cttcatcacc agatcgaccg
    10561 ccttagttgt cgtgcttatt acggcgggat gtatcagaaa catacgacgc atcacttgcg
    10621 tcacagagac ttgctcaagg gagatgagac gtgtctgagt tagttcatgt gcagcttggc
    10681 ggccctatgg ccaaacattt tggccgccac tggcatctaa aggtgcgcaa tacaaaacag
    10741 gctctggatt taattgaggc caataagcct ggttttaaag catggatgaa gcgcaatatc
    10801 aaaacctatg acagatacca catccagatc accaataaac agggccacaa gtggtctgtg
    10861 gacgagagtg aatatcagat gatggggcag tctgacaaca ttgccaaaat ccgcattacc
    10921 cctgttccgc gaggaagcgg cggatctgct tttgggtggt ttcagacggt agtaggggcc
    10981 gctttgttgg ttgtatcggc ggtaatgatg cccgctcttg cccctctcgg tttgtcactg
    11041 atgatgggcg gtatagcgca aatcatatct ccgcaagcca ctaacgaaag tgtgaggcag
    11101 gcggataact cgaactctta ttactttgat ggacctcaaa acacaacaaa ccaggggaac
    11161 cctgtacagc ttatctatgg cgaggaaatt ttagttggct cacaggtcgt gagttcttct
    11221 atcaccatcg accagcttat gtaatcaagg atttttggac atggaacagt tcaagaagaa
    11281 aaagttacct ctattaattg caggttctgg tggcaaaaaa agtagcaaaa gctctagtcg
    11341 cacaccagtt gaagcagacg ataccgtaaa ttctcgtgct atggcctcta ttctcgatct
    11401 tcttggagaa ggcgtaattg gcggcttggt aaatggcgca agatctattt ttgtcgacga
    11461 tttgcccata gtaaatgaag acggctcttc aaactttagc ggaatttcat gggattttcg
    11521 agacggctcg caagaccaga caccaatgtc tggctttgat ttcgtcgaaa cacctaaatc
    11581 gatcaatatc caactaaaga aatcgcatta tgtgacggtt tcaatagata acgatgaggc
    11641 cgatcgtgtt cgagtcatca tgaagttccc ttctctgcga agaatagacc aaaaaacggg
    11701 tgatacaaac ggcactaccg ttgagtataa gttccagatc tcaaatggag attcaacatt
    11761 tgtagatgtg gtcgcagaag gtgaaaaaaa cgttggtatt aagttaacag cgaagaaaac
    11821 cggtgcttat tatcgtagtt atgagctgaa gttacccaag ccaggtcggg catacagtgt
    11881 tcgagtagtt cgtattactg atgataacag cggtcagtat ctttataacg atacatgggt
    11941 ggactcaatc ggtgagatcg ttgatacacc gatgaactac ccaaactctg cgttggtggg
    12001 actgaaggtt aattctgagc agtttggcgg ctctatgcct tctcgttcct atctggtgcg
    12061 gggattaaaa attcgcgtac catcaaacta caacgaggct agcaacactt atgatggcgt
    12121 atgggatggc tcttttaagc tgttgtcatc ttcaaaccct gcgtggattc tcttcgactt
    12181 gcttaccaat tctcgatatg ggcttggtca gtatgtgtcg gaatctatga ttgatctcgg
    12241 ccaactgtac cagatcggtc gatattgtga cgaagaggtt gatgatggct ttggtggtaa
    12301 agagaaacgc tttgcaatca atacgcagat caccagtaga caggatgcat accgactaat
    12361 tcaggatatt gccggtgcat tccgcggcat ggtgttttgg gctggtggca tggttaacat
    12421 catgcaggat agcccatcag atccagtaat gatgtttacc aactcgaacg tcaaagatgg
    12481 attgtttacc tataaaggtt ctgcgcgtaa agatcgccca tccgttgcgc tcgtaaccta
    12541 caacaacaag gaggacggtt ataagcaaaa cattgagtac gttgaagatc aggacgcaat
    12601 gcgccgttat ggtgagcgta aaacagaagt cgtagcattt ggatgtacaa gccgaggcca
    12661 ggctcaccga gttggtttgt ggcttttgta taccgccaga atggagtcgg atgtaattac
    12721 atttactgcc ggtttagacg cctcatttct gatgcccggt gaaaccgttc tgattcagaa
    12781 caaatatcgt gcaggtaaac gtaactccgg tcgaattgtg gagtttacca aaaacagcat
    12841 cactctcgat gcgcctgtgt cgttagctaa aagcggcagc tttattcgga tattgaatca
    12901 ggaaggcaaa atcgttgaac gcgatgttct tgaaactggc gaaaacataa caaaggttac
    12961 gttttcaaaa gctctgtcgt cagcggaaac gcctgttttg aacggtgtat ggacaatcac
    13021 agaaccagat ctcgaaccta tgcgcgttcg catcgttaac atcgctcagg gggaaacgtc
    13081 gggtagcttt gacatcaccg ctgttgagaa caatccgtct aaatatgagg caatcgacaa
    13141 tggtgcaacg cttatcccgc agaatacgac ggtattggac ccgacttact ccaagccgtc
    13201 taatttgcaa atcaccgaag ggacttatct ctcaagccca ggcaacctgt cagtaaaact
    13261 gactgcaaca tgggaaggga aatctccaga gtattggatc agttggcgac gttctgatga
    13321 aaacaatgta tcgaactggc aatcggcacg tgtaaccgaa gagcaatacg aaatcgttaa
    13381 tgtcgcggag aatggacgct acgacttcca actgtatgcg gtttcattca acggtaaaaa
    13441 aacagagatt atcagtaccg tttatcaggt gctgggcaca atgacaccgc cggatgcacc
    13501 aacgtcatta acggccgttg gagactatcg taatgtgata ctgaattggg ttaatccaga
    13561 ttcggtagac cttgatcaca ttaatgttta cgcatcccag accaacaatc tggatacggc
    13621 gaaactgatc gcagagtctg caagcaccac ctttacacat tctggtcttg gggatagtga
    13681 aacttggtat tactgggtta gatcagcaaa taagcgtggc atgttgagtc gcccaaactc
    13741 aaatcttggt acagaggcca caacgcgaga tgttctttca tttttagcca ataaaattac
    13801 ctcgtcagaa cttggccagg ctttaaatga ggaaattgac agtaaggctt ctcaacaagc
    13861 tgttgatgaa atcaataatc atatcgatca gagcgtcgaa tctctggaag gtgaaatttc
    13921 cgcattaaat aaggagctaa cgagtagtat cgatgagttc aaaacatcgt ttactgaacg
    13981 cagcgagaca ctggagaatg cgcaaagcga gcttagaacg gaagtttcta gcactcggga
    14041 aaaagtagat gatgcattac gacaagttga aaattcaaat gcagcactta ttgaactacg
    14101 taatacagta tcaaaacaag gtgattctgt tgctggtgtc gtagaagcag caaatgcggc
    14161 acttgaaaat gcatcatctt taattgccga agagcgtaaa gttcgtgcac aagcagatct
    14221 agcgaatgct aaaaagatag aaacgatgag atcatcggtt gatgaaagtg ttgcggctgt
    14281 agaagaaatg cggagaacgg ttgctgaggt aggaagagta agttcagaaa tcacaactaa
    14341 tcttgaagcc atttctaaga caaatatcga tcttgcgttg cggcaggacg aagatcagca
    14401 cagacagctg gtaaataacg cgaaaattgc cacgactcaa aagattcttg tcgatgatgt
    14461 gtcagcaatg gcgtctaagg ttgaagagat acgagccgag attggtgaca acattcgagc
    14521 atctattctc gaagaagcga cagccagaac tgatggtgat aatgcgttag ccaatcgtgt
    14581 gacacaatta cagagtaaat ttgaaggtga tatcagtgcc gcaataagga ccgaacaatc
    14641 tgcaagagca tcggccaatg agactctttc aaagcagatc acacaactgg agtctaaagt
    14701 aaatactgat atttcaaccg ctattagaga agagcaacaa acaagagcaa cgcaagatga
    14761 agcattatca aggcaaataa ctcaacttca aacaaaagtt aataacgata tatctgctgc
    14821 tattaaaagt gagcaacaag cgcgagttag cggcgattca gcgttatcca cacaaattaa
    14881 tcaattgcag gcaaaagtag atgatgacat atcagctgct attaatgagg aaagagaagc
    14941 cagagcatca ggcgatagtg cactatcaag agaagttagt agtttaaaaa caaaaacagg
    15001 taaagacatt gcagcggcgg tagcagttga aaccaaagca agaactgacg ctaacgatgc
    15061 attatccaga caaatttctt ctcttactac gagggctaat aatcttcagt cctcgataga
    15121 gcgagaatcg acatctcgta caagtcagga ttctgccatt gcaaaagact tggcaagttt
    15181 taaaacaaaa actgctaagg atataagtgc agctgttgcg gttgaaactc aagcaagagc
    15241 tgccgctgat accgcattga cgtcgcagat taactcacta aagacacaaa caggtaaaga
    15301 tataaaggct gctgtagcag aagagacgaa agccagaact gatgctgatg cagcgctttc
    15361 gacaagggta actaatctcc agtcgcaaac aacaaattat atcaatgcag cgattacgtc
    15421 agagtcaaag gccagaacaa gtgcagacaa cgctctagga aaacgaattg atacactgaa
    15481 agcgtctgta gatggaaatg ttgccgttat tcagcagcag gcttctgcta tagctgacgc
    15541 taatagaaaa gtatcaacct cgtacacatt gaagatggaa acttcaacga gtggtgggca
    15601 gaagtatgta gcgggcattg cgctgggtat tgatacaacg gggctttctc agtttttggt
    15661 gcgggcagat cgatttggtt tggtcaacaa tgttaatggg aagatcacaa cgccatttgt
    15721 tatcgaaaac agcgttgcgt atatgaacgg tgcttatatc aaagatggca ctatcacaaa
    15781 cgcaaaggta ggtgacttac aatcaacaaa cttcgtaaga ggacgagccg gatggagatt
    15841 tggcaaaaac ggaacgcttg agattaacgg taacagtgga ggaaatggga gattagttat
    15901 caatgggcag agaatcgacg tttacgacga caataatgta ctcagggtaa gaattggtcg
    15961 actttgatta tggaaaaaaa tatttttatc gataataata ggtagttaca tacttaatgg
    16021 tgggcaagga tagcccacct gattaaagga gcagattcaa catgtggtac agggaaggta
    16081 ctatcacatt tacacaaggc agcaatactc tggttggtgc tgggacagca tggaacgtta
    16141 cggccaatgg tgtgttgcct ggcatgattg tcgtcgcacc tgataataaa ttgtatgaaa
    16201 ttaaaagcgt aacaaatgac acaaccttaa cgttggttga aaactatact ggtgaaacgc
    16261 aaagtgatat accatgtagg atcatcacta cctacgaagg agatttgaca cagtttagtg
    16321 cgagatttac ggctctttta tcacgaatgt ctggtgatgc aaaaacagtt cgaaattggt
    16381 taactgccgt agatgaagtt actcttgagc gagaagatgg cactaatgtt aatgtaaaac
    16441 ctctttctaa gattatcgcg gagcataata agcacgtaga atggtatgaa aataatacta
    16501 acgcaattaa ttccgcagga gataaagcaa aacaggctgc ggccagtgcg tcggctgcgg
    16561 cgaagagtgc gaatctagca agcagtaagg ctactcaggc atctaatagt gcgttggatg
    16621 ctacagcgtc taaaaatgca gcaaataaaa gtgcatcaag tgctaaagca tcagaaacaa
    16681 gtgctttaga ggcgaagaac gcagctgcaa tttctgcatc aacagcttcc actaaggcaa
    16741 cagaagccac aagttcggct aatgctgcgt tggtctctaa agaggctgca aaattatctg
    16801 aaatgaatgc tgcttctggt gctggcagtg catcaacttc tgcaaatcag gctagagagt
    16861 cggcaaggtc tgcgaagaca tccgctaatc aggcttcaac ttatgcgtct gctgctagag
    16921 cttcgaagga tgaagctagc cgaaagtctg ctgaagcctc aactagtgct agtaacgcat
    16981 catcgaaatc agctgaatct gcggctaaag cagaagaagc aaagcaaagc caaacatcag
    17041 cacttgcatc ggcagagcgt gcagagatag ctgctactag agcagaaaga gctttgtcag
    17101 cgatatcatt acatgatgcc agtttgacta tgaagggtat tgtcaaatta agcagtgaga
    17161 cgagcagttc gtcagaagaa tttgcagcaa caccaaaagc agtaaaggct gcgtttgatc
    17221 ttgctaaatc aaaatatact gcaaatgacg cgacaacttc acaaaaaggg ctggttcaat
    17281 taagtagcga tacaaataac gcctctgaat cattggccgc aacaacaaaa gcggttaaaa
    17341 gtgtaaagga tcttgctgat agaaaagcag caatcaacag cccagcattt actggcacac
    17401 caacggcacc aacggcgact caaggcacta ataatacgca aattgccaca actgcttatg
    17461 taagagaagc gatctcaggt cttgtaggat catcacctga agcgcttgat actcttaatg
    17521 agttggcaag ggctttgggt gatgatcccg attttgccac aacaatgacg aacaagttgg
    17581 ccacaaaaca acctcttgat gctacgctga ctgcactggc tggactaaca acagccgcta
    17641 ataaacttcc ttattttaca gggaatgata cagctggatt agcaacaata acttctgttg
    17701 gtcgagatat tctttctaaa gcaagtgtac aggcgattat tcagtatctt ggattgaagg
    17761 aaaatggaac aagtggcgat aaaatacctt tattgagtac agccaataca tggagtgcaa
    17821 agcaaacatt tagtaatggt cttgcaggct cacttaacgg aaattccgat accgcaacaa
    17881 aattaaaagc agcacgaaaa attggcggag taccgtttga tggttcatca gatataaatc
    17941 taccaggtgt aaactcaaca ggaactcaga gtactagtgg aaacgctgcc tctgccacta
    18001 agttagcaac acctcgttca attggtggaa tgttatttga cggcactgca aatattgact
    18061 taccaggcgt taacaaggtt ggaaatcaga gtacaacagg taatgcaggg agcgcaacga
    18121 gactgcaaac ggcaagaaca ataggtgggg tatcgtttaa tggcactgca aatattgact
    18181 tacctggtgt caataaagct ggaaatcaga acacgtcagg taatgctgca acggcgacaa
    18241 aacttgcaac ggcacgaaaa ataaatgggg tcagttttga tggctcaaag aatataagca
    18301 tcacgccaaa acaactaggt gcagcaaaat atgttgctaa tcttgctacg gggaatactc
    18361 aagagtggac aactgcacaa tttgtcgcat ggttaaattc acaaggggca tttgatgcaa
    18421 ctcagtggag cacacgttgt agctggtctt atggtagtaa ctgttatata ggaaatgcat
    18481 caacgggatg tggagttata cctcttgctg gttctgttat tgaggtgttt tcaaccggta
    18541 caagtaatta cactatacgc attacaacac cttcaacatc tggaggtgca tctggtgctg
    18601 ttcttaatag tgagtttatt tatacataca acggcgataa ctatagccca acatggcgtc
    18661 gtaattacag ttctcaaaat aaaccaacgg cttcagagat aggcgccctt gcctcaaatg
    18721 gaaatgcagt ttcggctacg aaactgaaaa cagcgagaaa tatagggggg gtatcgtttg
    18781 atggtacagc gaatatagat ttaccaggcg ttaacaaagc tggaaatcag aacacatcag
    18841 gtaatgctgc aacagctact aaattaaaaa catcaagaac aataggtgga gtatcatttg
    18901 atggtagcgc aaacattaat cttccaggtg tgaatacgtc aggaaatcag aatacatctg
    18961 gtaacgcagc atcagcaact aagctacaaa cagcacgtac tataaatgga gtaagtttcg
    19021 acggaacaaa aaatattagt ataagtgccg ctcaaattgg tgctttgcct atcggtgggg
    19081 gtacgataac aggtaatttg actgtaaatg gaaatggctc atttaatgat gttcagatcc
    19141 gctcagataa gcgtaacaag cgtaatctgg taaagctaga taatgcgtta gatcgtttgg
    19201 aggcacttac tggttatctt tatgaaatcc aactctctga tgatagttgg aaaacgtcag
    19261 ttggtttaat tgctcaggat gcacaaaaag cattgtctga actggtaact gaagacacag
    19321 acgttatatc tggtgaaaaa cgtctgtgtc ttaactacaa cggcataatt gcattgttag
    19381 tcgagggctt taaaacactt cgtcatgaga ttaaagaact tcgggagaag taaacgacag
    19441 ctgttggagt ttctggtttc tactgaattt aaattgtggg ggtgatactc accccacgca
    19501 ttcaaaagga ggggtaaaat gggggtagcg tcgggatggg tcggttcttc ggctaagagc
    19561 gaaacgggcg aacaatggat gagcgctgcg ggtactaagt taggattggg taaacctttt
    19621 atgatgagtc aaatggtggg acgatctatg ggctgtaaaa ttgcaaccgc ttactataaa
    19681 gggaatcctc ctgataaagt cgaaaactgg ggggctgtag gttcagattg gccattggct
    19741 gaaaagaacc aaggtacaat tgctggtgct tcgagctgtg gtttaggtcg tttagtgggg
    19801 atcgtgctta cgcaagctca ctacgttcca ggtttaccgc ccactggggg tttatacatc
    19861 gcagggggaa gggcaagcaa tattacagta acagttggtg gagtctcaca agtttttact
    19921 tacaatagtg ttattaacgg ttttcactat tactggcaag gaacacctag ttcggccttt
    19981 ataacggcaa
//
LOCUS       DAZOZD010000064        19408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOZD010000064 DAZOZD010000000
VERSION     DAZOZD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.064"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUPB_001815"
     CDS             <1..245
                     /locus_tag="ACHUPB_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0758643.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUPB_001816"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUPB_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0758644.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUPB_001817"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUPB_001817"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR0758645.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUPB_001818"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUPB_001818"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR0758646.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUPB_001819"
     CDS             3423..4061
                     /locus_tag="ACHUPB_001819"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0758647.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUPB_001820"
     CDS             4066..4842
                     /locus_tag="ACHUPB_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR0758648.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUPB_001821"
     CDS             4931..6295
                     /locus_tag="ACHUPB_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR0758649.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUPB_001822"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUPB_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0758650.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUPB_001823"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUPB_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0758651.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUPB_001824"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUPB_001824"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0758652.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUPB_001825"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUPB_001825"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0758653.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUPB_001826"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUPB_001826"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0758654.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUPB_001827"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUPB_001827"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0758655.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUPB_001828"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUPB_001828"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0758656.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUPB_001829"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUPB_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0758657.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUPB_001830"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUPB_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0758658.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUPB_001831"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUPB_001831"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0758659.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUPB_001832"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUPB_001832"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0758660.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUPB_001833"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUPB_001833"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0758661.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUPB_001834"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUPB_001834"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0758662.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHUPB_001835"
     CDS             complement(17716..18009)
                     /gene="cas2e"
                     /locus_tag="ACHUPB_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR0758663.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHUPB_001836"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="ACHUPB_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="ACHUPB_001837"
     CDS             complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="ACHUPB_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0758664.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4592 a         5230 c         4848 g         4738 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagcg
//
LOCUS       DAZOZD010000065        18844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOZD010000065 DAZOZD010000000
VERSION     DAZOZD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.065"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHUPB_001838"
     CDS             complement(<1..103)
                     /locus_tag="ACHUPB_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758665.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHUPB_001839"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="ACHUPB_001839"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0758666.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHUPB_001840"
     CDS             complement(2317..2607)
                     /gene="hupB"
                     /locus_tag="ACHUPB_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0758667.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHUPB_001841"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="ACHUPB_001841"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0758668.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHUPB_001842"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="ACHUPB_001842"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0758669.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHUPB_001843"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="ACHUPB_001843"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0758670.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHUPB_001844"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="ACHUPB_001844"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0758671.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="ACHUPB_001845"
     CDS             complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="ACHUPB_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0758672.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9873..10451
                     /locus_tag="ACHUPB_001846"
     CDS             9873..10451
                     /locus_tag="ACHUPB_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0758673.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10495..11970
                     /gene="ampG"
                     /locus_tag="ACHUPB_001847"
     CDS             10495..11970
                     /gene="ampG"
                     /locus_tag="ACHUPB_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0758674.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12431..13378
                     /gene="cyoA"
                     /locus_tag="ACHUPB_001848"
     CDS             12431..13378
                     /gene="cyoA"
                     /locus_tag="ACHUPB_001848"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR0758675.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13400..15391
                     /gene="cyoB"
                     /locus_tag="ACHUPB_001849"
     CDS             13400..15391
                     /gene="cyoB"
                     /locus_tag="ACHUPB_001849"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR0758676.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15381..15995
                     /locus_tag="ACHUPB_001850"
     CDS             15381..15995
                     /locus_tag="ACHUPB_001850"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0758677.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15995..16324
                     /locus_tag="ACHUPB_001851"
     CDS             15995..16324
                     /locus_tag="ACHUPB_001851"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0758678.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16336..17226
                     /gene="cyoE"
                     /locus_tag="ACHUPB_001852"
     CDS             16336..17226
                     /gene="cyoE"
                     /locus_tag="ACHUPB_001852"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0758679.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17411..17722
                     /locus_tag="ACHUPB_001853"
     CDS             17411..17722
                     /locus_tag="ACHUPB_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0758680.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17706..18248
                     /locus_tag="ACHUPB_001854"
     CDS             17706..18248
                     /locus_tag="ACHUPB_001854"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0758681.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18304..>18844)
                     /locus_tag="ACHUPB_001855"
     CDS             complement(18304..>18844)
                     /locus_tag="ACHUPB_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0758682.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4955 c         4580 g         5093 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcagcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ttcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt
     9121 cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg
     9181 gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc
     9241 aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt
     9301 cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag
     9361 tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac
     9421 ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat
     9481 tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt
     9541 tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca
     9601 tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt
     9661 taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac
     9721 acaggcctcg catcagcgtt tcttttacca ggggtgatta tttcgccaac atgatgaatt
     9781 tacatgcctt acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc
     9841 aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag
     9901 ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga
     9961 cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg
    10021 ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc
    10081 tgaccgcctc ccgagatctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg
    10141 cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac
    10201 tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg
    10261 ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca
    10321 acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc
    10381 tgaccgatac catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga
    10441 acgcgcgtta atttctgccc tctggcccgg tgcaaaccgg gcctgtagac gcccatgtcc
    10501 agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt
    10561 gcttccgggc taccgctcgc cctgacatcc ggtaccttac aggcctggat gacggtcgag
    10621 aatattgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa
    10681 ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt
    10741 tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa
    10801 cctggcaccc aactccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctcggcc
    10861 tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt
    10921 gcgggcgcgg caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta
    10981 gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg
    11041 ctgttgatcc cttgtattat cgcgacgttg cttgcaccag aaccgaccga caccattcct
    11101 gtgccaaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat
    11161 aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg
    11221 agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg
    11281 gttaacaaaa cgcttggctt actggcgacc attgtgggcg cattgtacgg tgggattttg
    11341 atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct
    11401 aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc
    11461 gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc actgttaatg
    11521 acgctgtgta ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct
    11581 gtaggacgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg
    11641 acattctatc tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc
    11701 cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg
    11761 acaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg
    11821 ttactgctgt tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg
    11881 ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat
    11941 ctggctctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca
    12001 ctcgccgtaa ttattacggc taaataaaca tcagtatcgc ttaataatat ttgttctttt
    12061 gtgcggctta gcgtttggaa attattgacg caatttatga attctatttt ttcttacacg
    12121 attcagctaa tgagtcttta ttttcccccc acccaattgt caccataatg ataattattt
    12181 gttaaacaat tgttttattc aagattggtt ataccaattg cccgtccaaa ctccttctgc
    12241 actccccttt cgttataacg cccttttgca acagcttctt aaaatcaact tgatatgttt
    12301 tgcaacatat gtaacctgac agccaaatcc aagtaacagg aatttaatca tgtttacagt
    12361 aatgtaacct ccccgtaaaa tgcccacaca ctttaaacgc caccagatcc cgtggaattg
    12421 aggtcgttaa atgagactca ggaaatacaa taaaagtttg ggatggttgt cattatttgc
    12481 aggcactgta ttgctcagtg gctgtaattc tgcgctgtta gatcccaaag gacagattgg
    12541 tctggagcaa cgttcactga tactgacggc atttggcctg atgttgattg tcgttattcc
    12601 cgcaatcttg atggctgttg gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa
    12661 gtacagcccg aactggtcac actccaataa agtggaagct gtggtctgga cggtacctat
    12721 cttaatcatc atcttccttg cggtactgac ctggaaaacc actcacgctc ttgagcctag
    12781 caagccgctg gcacacgacg agaagcccat taccatcgaa gtggtttcca tggactggaa
    12841 atggttcttc atctacccgg aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc
    12901 gaacactccg gtgtacttca aagtgacctc caactccgtg atgaactcct tcttcattcc
    12961 gcgtctgggt agccagattt atgccatggc cggtatgcag actcgcctgc atctgatcgc
    13021 caacgaaccc ggtacttatg acggtatctc cgccagctac agcgggccgg gcttctcagg
    13081 catgaagttc aaagctattg caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa
    13141 agcgaaacag tcgccgaaca ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc
    13201 tagcgaatac aaccaggtgg aatatttctc caacgtgaaa ccagacttgt ttgccgatgt
    13261 gattaacaag tttatggctc acggtaagag catggacatg acccagccag aaggtgagca
    13321 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
    13381 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
    13441 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
    13501 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
    13561 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
    13621 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct
    13681 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
    13741 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
    13801 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggtgtgat
    13861 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
    13921 accgctatcg ggaatagagt acagtccggg agtcggtgtc gattactgga tatggagtct
    13981 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
    14041 gatgcgcgca ccgggcatga ccatgttcaa gatgccagta tttacctggg catcactgtg
    14101 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
    14161 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
    14221 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
    14281 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
    14341 ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca
    14401 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
    14461 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
    14521 catcgtgttc cattctgcga tgctgtggac catcggtttt atcgtcactt tctcggtggg
    14581 cgggatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
    14641 gttcctgatt gcgcacttcc ataacgttat catcggcggc gtggtcttcg gctgcttcgc
    14701 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaatgaaa cctggggtaa
    14761 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
    14821 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
    14881 gctgatgatt gcagctagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
    14941 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
    15001 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
    15061 tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
    15121 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
    15181 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
    15241 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
    15301 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
    15361 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
    15421 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
    15481 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
    15541 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
    15601 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
    15661 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
    15721 ggatggaaat ctacgaattc catcacctga ttgttaacgg catgggtccg gatcgcagcg
    15781 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc
    15841 ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg cggcctgacc agcactaacc
    15901 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
    15961 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
    16021 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
    16081 ggtgattccg ttctggatgg tgatgacagg agctgcctct ccggccgtaa ttctgggaac
    16141 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
    16201 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
    16261 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
    16321 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
    16381 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
    16441 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
    16501 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
    16561 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctaggcttt
    16621 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
    16681 ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga aacgccactc ggtctacggc
    16741 acattgattg gttcgctctc cggcgctgcg ccgccggtga ttggctactg tgcagtaacc
    16801 ggcgagttcg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
    16861 cactcctatg ccatcgccat tttccgcttt aaggattatc aggcggcaaa cattccggta
    16921 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
    16981 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
    17041 gtcgccgcgg cggtgagcgt ctggtggtta ggtatggccc tgcgcggtta taaagttgct
    17101 gatgacagga tctgggcgcg caagctgttc ggcttctcta tcatcgccat cacagccctc
    17161 tcggtgatga tgtccgttga ttttatggtg ccggactcgc atacgctgct ggctgctgtt
    17221 tggtaacaca acctctctat taaaaaggtg ctacggcacc ttttttattg tctgagatca
    17281 gagatacggc taataaccgt atgtcttgac agagtgagca gatgaacata aattaatcat
    17341 acggttgata gccgtattat gtatgagatc gactccctac gatttcattc agttaatcaa
    17401 agaggtcata atgcctggaa aaacagccac tctacccgat gtcgataaaa cgctgaaaaa
    17461 tgcgcgggtc gaactcaaaa ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca
    17521 agccgttggg gtagatttaa gcgcttttat tttgagcgct gctatggaac gcgccgaaag
    17581 tgtgcttgat aaccaacgtc gccgtgagct ttcgaatcaa agctgggaac tgatgaacca
    17641 actcatcgct gaacctgctc aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa
    17701 cagcgatggt cgacaagcat gaagagatta ctctgcccat agtcctctcc tgtaattatc
    17761 agtctgatat tacttatcct gggcaaaaac agtttgattg cggtaaccct gttatcgata
    17821 aatttgtacg cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac
    17881 ttattgacag acaaagtggt gaactgatcg gcatctgtac ttttacggca tattcgctgg
    17941 aaaaacaacg cgtttctggc gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca
    18001 gattagtcat gttgggggta gcacggaagt atcaaaagcg gggctttggt caggacctac
    18061 tatgtgattt ttttgaacat gtaaaaataa ttcaccaggc attaccaatt aaaggggttt
    18121 atcttgatgc tgaccctgcc gccattaatt tttatgctcg tctcggcttt gttcagcttt
    18181 cagcgacacc aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg
    18241 cggcttagat tattatcgcc acccgccagg gcaagttttg ccttagccgg gggctgagca
    18301 gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat
    18361 ggttgccgtt gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc
    18421 cggtttataa agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa
    18481 gctaatattc agccggttta aaccgaaagg cgcgaagcca accgtttggt taatctcctt
    18541 accggatggg cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt
    18601 ggtggtggcg aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatctcgcat
    18661 cccagaacgg gtaaccgtaa tcacgggtaa cgtatcgtca accgtttttc tgtcaatcat
    18721 gtcttttcga caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc
    18781 aagatattcc ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacataat
    18841 gccc
//
LOCUS       DAZOZD010000066        18748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOZD010000066 DAZOZD010000000
VERSION     DAZOZD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.066"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            119..685
                     /gene="yqaB"
                     /locus_tag="ACHUPB_001856"
     CDS             119..685
                     /gene="yqaB"
                     /locus_tag="ACHUPB_001856"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0758683.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            682..1110
                     /locus_tag="ACHUPB_001857"
     CDS             682..1110
                     /locus_tag="ACHUPB_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0758684.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1183..2739
                     /gene="gshA"
                     /locus_tag="ACHUPB_001858"
     CDS             1183..2739
                     /gene="gshA"
                     /locus_tag="ACHUPB_001858"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0758685.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            2889..3404
                     /gene="luxS"
                     /locus_tag="ACHUPB_001859"
     CDS             2889..3404
                     /gene="luxS"
                     /locus_tag="ACHUPB_001859"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0758686.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3468..5006)
                     /gene="emrB"
                     /locus_tag="ACHUPB_001860"
     CDS             complement(3468..5006)
                     /gene="emrB"
                     /locus_tag="ACHUPB_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0758687.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5023..6195)
                     /gene="emrA"
                     /locus_tag="ACHUPB_001861"
     CDS             complement(5023..6195)
                     /gene="emrA"
                     /locus_tag="ACHUPB_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR0758688.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6322..6852)
                     /gene="emrR"
                     /locus_tag="ACHUPB_001862"
     CDS             complement(6322..6852)
                     /gene="emrR"
                     /locus_tag="ACHUPB_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0758689.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6943..7278)
                     /gene="ygaH"
                     /locus_tag="ACHUPB_001863"
     CDS             complement(6943..7278)
                     /gene="ygaH"
                     /locus_tag="ACHUPB_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0758690.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7268..8005)
                     /gene="ygaZ"
                     /locus_tag="ACHUPB_001864"
     CDS             complement(7268..8005)
                     /gene="ygaZ"
                     /locus_tag="ACHUPB_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0758691.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8129..9313)
                     /locus_tag="ACHUPB_001865"
     CDS             complement(8129..9313)
                     /locus_tag="ACHUPB_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0758692.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9604..10596)
                     /gene="proX"
                     /locus_tag="ACHUPB_001866"
     CDS             complement(9604..10596)
                     /gene="proX"
                     /locus_tag="ACHUPB_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0758693.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10654..11718)
                     /gene="proW"
                     /locus_tag="ACHUPB_001867"
     CDS             complement(10654..11718)
                     /gene="proW"
                     /locus_tag="ACHUPB_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0758694.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11711..12913)
                     /gene="proV"
                     /locus_tag="ACHUPB_001868"
     CDS             complement(11711..12913)
                     /gene="proV"
                     /locus_tag="ACHUPB_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0758695.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13269..14228)
                     /gene="nrdF"
                     /locus_tag="ACHUPB_001869"
     CDS             complement(13269..14228)
                     /gene="nrdF"
                     /locus_tag="ACHUPB_001869"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0758696.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14238..16382)
                     /gene="nrdE"
                     /locus_tag="ACHUPB_001870"
     CDS             complement(14238..16382)
                     /gene="nrdE"
                     /locus_tag="ACHUPB_001870"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0758697.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16355..16765)
                     /gene="nrdI"
                     /locus_tag="ACHUPB_001871"
                     /pseudo
     CDS             complement(16355..16765)
                     /gene="nrdI"
                     /locus_tag="ACHUPB_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(16762..17007)
                     /gene="nrdH"
                     /locus_tag="ACHUPB_001872"
     CDS             complement(16762..17007)
                     /gene="nrdH"
                     /locus_tag="ACHUPB_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0758698.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17255..17584)
                     /locus_tag="ACHUPB_001873"
     CDS             complement(17255..17584)
                     /locus_tag="ACHUPB_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR0758699.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17736..18080
                     /locus_tag="ACHUPB_001874"
     CDS             17736..18080
                     /locus_tag="ACHUPB_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0758700.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18117..18566)
                     /gene="alaE"
                     /locus_tag="ACHUPB_001875"
     CDS             complement(18117..18566)
                     /gene="alaE"
                     /locus_tag="ACHUPB_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0758701.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4686 a         4745 c         4855 g         4462 t
ORIGIN      
        1 tctcatttct aaataactcc tttatccatt tcctgaattt tcttctcctt cgccatcagc
       61 gacaaaatca agcaattacg ctaaagtagc gtcatatttt tcgacttgcg agaagaccat
      121 gtacgagcgt tatgcaggtt taatttttga tatggatggc acaatcctgg atacggagcc
      181 tacgcaccgt aaagcgtggc gcgaagtatt agggcactac ggtcttcagt acgatattca
      241 ggcgatgatt gcgcttaatg gatcgcccac ctggcgtatt gctcaggcaa ttattgagct
      301 gaatcaggcc gatctcgacc cgcatgcgtt agcgcgtgaa aaaacagaag cagtaagaag
      361 tatgctgctg gatagcgtcg aaccgcttcc tcttgttgat gtggtgaaaa gttggcatgg
      421 tcgtcgccca atggctgtag gaacggggag tgaaagcgcc atcgctgagg cattgctggc
      481 gcacctggga ttacgccatt attttgacgc cgtcgtcgct gccgatcacg tcaaacacca
      541 taaacccgcg ccagacacat ttttgttgtg cgcgcagcgt atgggcgtgc aaccgacgca
      601 gtgtgtggtc tttgaagatg ccgatttcgg tattcaggcg gcccgtgccg caggtatgga
      661 cgctgtggac gttcgcttgc tgtgagtgaa gcgttatcgc ttttctcgtt gtttgccagt
      721 agttttctca gcgctacact attacccggc aactccgaag tcgtgctggt ggcaatgttg
      781 ctttccggga tcagtcatcc ctgggtttta gttttaacag caacaatggg taatagcctt
      841 ggagggttaa ctaacgttat ccttgggcgt ttctttccat tgcgtaaaac atcgcgctgg
      901 caagagaaag ctatcggctg gctgaaacgc tatggtgcag tcacgctatt attaagctgg
      961 atgcccgtgg ttggcgattt actgtgtctg ttagcgggat ggatgcgcat ctcgtgggga
     1021 ccggtaatct tttttttgtg ccttggtaaa gcgttacgct atgttgcagt tgcagcagcg
     1081 accgttcagg gcatgatgtg gtggcactaa ttgtaggcct gcacatatgg tcaccattac
     1141 agttatgcta attaaaacga ttttgacagg cgggaggtca atttgatccc ggacgtatca
     1201 caggcgctgg cctggctgga aaaacatcct caggcgttaa aggggataca gcgtgggctg
     1261 gagcgcgaaa ctttgcgtgt taatgctgat ggcacactgg caacaacagg tcatcctgaa
     1321 gcattaggtt ccgcactgac gcacaaatgg attactaccg attttgcgga agcattgctg
     1381 gaattcatta caccagtgga tggtgatatt gaacatatgc tgacctttat gcgcgatctg
     1441 catcgttata cggcgcgcaa tatgggcgat gagcggatgt ggccgttaag tatgccatgc
     1501 tacatcgcag aaggtcagga catcgaactg gcacagtacg gcacttctaa caccggacgc
     1561 tttaaaacgc tgtatcgtga agggctgaaa aatcgctacg gcgcgctgat gcaaaccatt
     1621 tccggcgtgc actacaattt ctctttgcca atggcattct ggcaagcgaa gtgcggtgat
     1681 atctcgggcg ctgatgccaa agagaaaatt tctgcgggct atttccgcgt tatccgcaat
     1741 tactatcgtt tcggttgggt cattccttat ctgtttggtg catctccggc gatttgttct
     1801 tctttcctgc aaggaaaacc aacgtcgctg ccgtttgaga aaaccgagtg cggtatgtat
     1861 tacctgccgt atgcgacctc tcttcgtttg agcgatctcg gctataccaa taaatcgcaa
     1921 agcaatcttg gtattacctt caacgatctt tacgagtacg tagcgggcct taaacaggca
     1981 atcaaaacgc catcggaaga gtacgcgaag attggtattg agaaagacgg taagaggctg
     2041 caaatcaata gcaacgtgct gcagattgaa aacgaactgt atgcgccgat tcgtccaaaa
     2101 cgcgttaccc gcagcggcga gtcaccttct gatgcgctgt tacgtggcgg cattgaatat
     2161 attgaagtgc gttcgctgga catcaacccg ttctcgccga ttggtgtaga tgaacagcag
     2221 gtgcgattcc tcgacctgtt tatggtctgg tgtgcgctgg ctgatgcacc ggaaatgagc
     2281 agtagcgaac ttgcctgtac gcgcgttaac tggaaccggg tgatccttga aggtcgcaaa
     2341 ccgggcctga cgctgggtat cggctgcgaa accgcgcagt tcccgttact gcaggtgggt
     2401 aaagatctgt tccgcgatct gaaacgcgtc gcgcaaacgc tggatagcat taacggcggc
     2461 gacgcgtatc agaaagtgtg tgatgaactg gttgcctgct tcgataatcc cgatctgact
     2521 ttctctgccc gtatcttaag gtctatgatt gatactggta ttggcggaac aggcaaagcg
     2581 tttgcagaag cctaccgtaa tctgctgcgt gaagagccgc tggaaattct gcgcgaagag
     2641 gattttgtag ccgagcgcga ggcatctgaa cgccgtcagc aggaaatgga aaccgctgat
     2701 accgaaccgt ttgcggtgtg gctggaaaaa cacacctgac agaaaagaaa aaggccactc
     2761 gtgagtggcc aaaatttcat ctctgaattc agggatgatg ataacaaatg cgcgtctttc
     2821 atatactcag actcgcctgg gaagaaagag ttcagaaaat ttttaaaaaa attaccggag
     2881 gtggctaaat gccgttgtta gatagcttca cagtcgatca tacccggatg gaagcgcctg
     2941 cagttcgggt ggcgaaaaca atgaacaccc cgcatggcga cgcaatcacc gtgttcgatc
     3001 tgcgcttctg cgtgccgaac aaagaagtga tgccagaaag agggatccat accctggagc
     3061 acctgtttgc tggttttatg cgtaaccatc ttaacggtaa tggtgtagag attatcgata
     3121 tctcgccaat gggctgccgc accggttttt atatgagtct gattggtacg ccagatgagc
     3181 agcgtgttgc tgatgcctgg aaagcggcaa tggaagacgt gctgaaagtg caggatcaga
     3241 atcagatccc ggaactgaac gtctaccagt gtggcactta ccagatgcac tcgttgcagg
     3301 aagcgcagga tattgcgcgt agcattctgg aacgtgacgt acgcatcaac agcaacgaag
     3361 aactggcact gccgaaagag aagttgcagg aactgcacat ctagtcagta aactatcttc
     3421 acaattaatt gaaaaaagcc agttcaaatg aactggctta gttgtactta gtgcgcaccg
     3481 ccgccaccgc cacctgcgcc aaatggcggt ttagcaaacc acaccagccc cagcagtacg
     3541 aggaatatcc cggctgacat ccagaagatc tcattggcgg aaataatcag cccctgattg
     3601 gtgatctgct gggcaatcca gcctgatgcc tgctgttgcg tcatcccaag cccttccagt
     3661 tgactgtaca tcgcctgggc attcgggttg aacgggttta ccgactcagt caactgcgca
     3721 tggtgcatcg actcgcggtt ggtccacatg gtcgtggtta tcgacgtgcc gatagacccc
     3781 gccagcgttc gcgtaaagtt agagaggctc gatgccgccg ccagtcgttc cggtggcaaa
     3841 ccagacagcg taatggtggt cagcggcata aagaagcagg ccaccgcaaa cccctggata
     3901 aactgcggcc aggccgacgc gccaaaatcc atacctggtt caaaggtata ggcacgccag
     3961 tagaagcaga cggcatacat aataaagctg aaggttacca gccgccgcat atccagttta
     4021 tgcgcgaagc ggccgataat cggcgacagg atcaccggaa taatccctac cggcgcagag
     4081 gccaaacctg cccaggtcgc cgtgtaaccg tagacctcct gcaacaactg cggcagcaga
     4141 acaatagcgc cgaagtagag catatacgcg aggctgatac acaagcagcc gatggtgaag
     4201 ttgcgcgact taaacaacga cagatcgact atcgggttat cgtcggtcag ctcccagaca
     4261 atcaggaagc agatagccac caccgccacc acggtaagga tgataatttc ctgtgatgaa
     4321 aaccagtcca gctctttacc gcggtcgagc ataatctgca ggctgccgat accaataacc
     4381 agcagtgcca gccccacggc atcaatccgc cgccgtttgg tgcgggtttc acgtccgcgc
     4441 agagtttgca gtgtcatcaa caccaccgcc acgccaatcg gcacgttgat gaagaatatc
     4501 cagccccagt ggtaattatc gctgatataa ccgccgagga tcgggccgca aattggcgcg
     4561 acaatcaccg tcatcgacca caacgccagc gcgatcgagc gtttggctgg cgggtagtta
     4621 ttcagcaata gactttgcga aagcgggatc aacggcccgg cgacaatccc ctgaatcacg
     4681 cggaagaaga tcagcatatt caggctgctg gagacaccac acgcccacga cgcaatagca
     4741 aaggcgatgg tggaccaaag gaacagtttc acttccccga cgcgctttgc cagccagccg
     4801 gtaagcggga tcgagatggc attcgccacc ccgaaagaag tgattaccca cgttccctgg
     4861 ctgagcgatg agcccagatt cccggcgata gtggggatcg ccacgttagc aatggtggag
     4921 tccagcacct gcatgaatgt cgccagtgac agcgcaatcg tcataatgac cagttgcgcg
     4981 ccttccagcg gtttttgctg ttgcatcaca cgcacctctg gattagccag cgttagcttt
     5041 tacgatatcg tcgatcagtt tattgacagg tgccaggctg atttcacgcg cggtacttac
     5101 cgcgaccggt gttgaacgta ctttatttgc cagtacctga ccgtcacggt tagtggtatt
     5161 gacgctcacc agtgtggaca aaccaatgcg cagcgggtat tgctccagct gtttctggtc
     5221 cagttcgata cgcacaggta gacgctgaac gactttgatc cagttaccgg tcgcattttg
     5281 cgccggaagc agtgagaatg cgctacctgt gcccatatcc agaccaacca ctttaccggt
     5341 gtatttcaca tcatcgccgt aaatatccgt ggtgatagtg accggctgac cgatacgcat
     5401 attggcaatc tgcgtctctt taaagttggc atccacccac atattggtgg ctggaacgac
     5461 cgccatcagc ggcgtcgttg ggctaatttg cgccccaggc tgtaccgcac ggcgggagac
     5521 ataaccggtc atcggactga caatacgggt acgctccagc gccagccagg cgttacgtac
     5581 ttcggtggca gcctgttgca cggcaggctg atcttccagt ttagtcccca gaatcatcgc
     5641 ctgattggca ttgtattgtt gaatagcgac gtccagttgc gcctgggcac tggtgacggc
     5701 gtcgcgggcg tgttgcagct cttcgcgacc aatcaggttg gcattgccca gcggcacacg
     5761 gcggttgtag tcgctttgtg cttttgcgag ggcgattttc tgcacctcaa tattcgcctg
     5821 caactgtttg ctgttaatca tcagctggtg ggtttggcga acgctggaag ccagtgcagt
     5881 tttggctttt tcaaacgcct ggcgagcatc tgtcgggtcg agagtgacca gcacgtcgcc
     5941 ttcttttaca aaatcggtgt tatcggccca gactttcgtc acgctgccag acacctgaga
     6001 cataatttga atctgattcc ctgccacgta tgcgtcatcg gtttcttcga aatgacgcag
     6061 taccaaaaac caataaatcc ctatcgctac ggcaataatt ataaagagca aggtgagaag
     6121 gaggagcaga cgcttacgtt tgccgctctt ctttaccggt tgctgcgggg tttgagtctc
     6181 cgcatttgcg ctcatattgt tctccacgat cttcttaact catcggctga gccgacctgc
     6241 ttgccaaaaa ggccagcatg ttgcgatgct ggccggtcat tttttctttt ataaatctgg
     6301 atttttgagc gagatgacgc gttagctcat cgcttcgaga accacaccgt cttgttccat
     6361 ctggtcgaga cgggagagca atttgcgggt gatttgctcg agctgatctt tttctgttgt
     6421 gctgagcgcg gaccagagtt gatgcaggca gttatgctgc ggtggtaaaa cctcgcgcaa
     6481 aaactcgtga cctttttccg ttaattgcag atgcaggcag cggcgatcgt tatcgctttc
     6541 acgacgttcg atccaaccgc gtttttccag ttcatcggca atacgcgtcg cgttggtacg
     6601 ggatgatcca agagcacaac ttaattcaga aggctgaata ctgtggtttt cctgagactc
     6661 cagcgtaatc aacgccataa acaacgtctc gttaatcccc tgagccttca gcattttatt
     6721 gcggttctcc agcagcttgc tttgcatgtg catgcaaaga cgagtcagaa ggatctcctg
     6781 ataaggaaaa tcttcgtggc ggctggcgcg aaattttagc atttgttcaa tgggcgtaaa
     6841 cgaactatcc atttgggtat gacctcatta atttcagccg atatagtaac gacagtgaca
     6901 aataaagtaa atgtattgtt ttagaaaaat gattcttgtg ggttatataa tcgccatcac
     6961 tttccaggcg agcccatagg ccagcgcact aagcagtgtt gggatgataa tgctgcgtgt
     7021 tttatagaaa ctggcaccca gtaccgcgaa gccgaccagc gtgggcacga aacggcgtgt
     7081 atcgtgcatc acttctggtg cggtagagac aaccagcaga gcgcatatcg aggcgatgcc
     7141 aatggtgtcg agcaaaatac ctaccgcgcc acgtttggtt gggcgggcat tacccacacg
     7201 caggcgcagc ggcaaatagc ggaagcaata attcgccgcg ccaaccagta acccaggcag
     7261 cagaacctca tagctcatcg ggcgctcctt gccagaatgc ttggattaac gcagtgaggc
     7321 agccacagac aatgcctgcc agaatggcga cgggaataga aaatagcgtt acgcctgcaa
     7381 gggcaccaac taacgctgcg gtaacgcaaa gagattgttt gcgctggaaa gaggcgagca
     7441 ggaaactcat aaagagtgcc ggaagcataa aacctaatgc tgcttcaacg gcgggataac
     7501 cttgcagcaa gccgctgcca gagaatgccc ctattaccgt accaaatacc cacgatgacc
     7561 atgaactgaa ggcaatgccg atcatccagt tctcgctcca gcggcgatta ttgcgtacca
     7621 gttttgctgt tgcggcggca aaaacctcat ccgtcaggcc aaacgcccac agggcggttt
     7681 tcgatttttg cagacgctga ataatacggc tacgcagtga cgggccatac aacacatggc
     7741 gaacatccat tgccatgacg gtcagtgcag caacccacaa actactcccg gctgccagca
     7801 tcgcggtaat gacgaactgg ctcgcgcctg cataaatgat gcaggagaaa aaaacgcttt
     7861 cgagaggaga gaatcccaga cgggtcgcat tcagaccgaa cgcaaaggcc accggaatat
     7921 aactaataac aatcggtaaa ctgtctttgc atccttccat gaaggtcgcc gaaccaggag
     7981 caggctgtgg agtagggctt tccatagagt gtacgcttaa cgattgttaa tattataaat
     8041 agactgaatg aatatcttaa ccttatcaga ctgatgggct tcttaacacc cttataagtg
     8101 taaagccacg aaaacggttg ctgattgctt aatttgcggc ttcctgacga tgaaaacctc
     8161 gccaccagac cagtaagtta actagggcaa tcgtcgcgcc agccagacaa acgccagccc
     8221 aaccgccatg ttgccaggct gaggctgaaa ttagcgaacc ggcggcaccg ccaataaagt
     8281 agctggtcat gtaacctgcg gtcaggcgat tacgcgcatc agggtgtatt cgataaatta
     8341 ccgtctggtt agtgatatgc acgccctgta cggtgagatc cagcaccagg atgccgataa
     8401 tcagcgccag tacggaagtg tgaccaaacc agatcgccag ccatgaaagt aatagcagca
     8461 gcagaccgaa agttgtggtg tggtgcgatt tacccttatc ggcaaaaccg cccgccggac
     8521 gagcgcccaa cgctccggca gctcccgcaa gtccaaacag accaatgaca ccatcgctgt
     8581 agttaaaagg tggagcggca agcaaaaagg ccattgaggt ccagagaatg ctgaagttgg
     8641 caaaggtcag gcagcccagc aatgcgcggg tgcggaggat cttatcgctg ataaacatgc
     8701 tgaaaacgga acctaacaac tgtgggtagt tgaggtgggt ttctgatttc atttgtggca
     8761 gaccacgcca taatgctagc gccatcagtg ccattaacac cgaggcaacc caaaagacgg
     8821 tgcgccagcc gccgaggttt gccagtaacc cggcaacggt tcgtgccagc aagatcccca
     8881 acagcagacc gctcataatg gtgccaacca ctttgccgcg tttatccggt gaagccagcg
     8941 tcgctgccag cggaaccaga atttgtgcca cgactgagaa taaaccggtt aatgccgtac
     9001 cgaggatcat catcgccagc gactgactgc tggcagtgat taacatgccg cctgccgcca
     9061 gtaaggtcat cgagacaatc aggcggcggc gttcaaacat atcaccgagg ggaacaagaa
     9121 acagcagacc tgctgcatag cccaactgcg cggcggtaac aataaagcct gccgaactgg
     9181 cggaaaggga aaagttacgc gcgatggtgt cgagcaatgg ctgggcgtaa tagttactgg
     9241 ctaccgcctg accggtggcg atagacatca gcacgatcag cgccgggcta agctcatgat
     9301 taggtttagt cattattttt gttcatcagg tgatgattaa gctggaaatc aataatagcg
     9361 gatgttgatg aatgggggcg aaagatagct tgttgaaatg tgcgatgtgg tttgtaggca
     9421 tgataagacg cgccagcgtc gcatcaggca ttgtgcacag aacggcggat gcggcgaaaa
     9481 cgccttatcc gccatacgga atgagtgtat tttgtaggca tgataagacg cgccagcgtc
     9541 gcatcaggca ttgtgcacag aatggcggat gaggcaaaaa cgacttatcc gcccgaataa
     9601 aaattacttc tgcgctgcca gcgcctcatt cacccagcca tcgaactgct gctggtgggc
     9661 tttgatccaa ccatcaacat ggccctgaat atcgccttct gaggctttgc cgtcatgcat
     9721 aatggcgttc tgggcgttaa tatctgccac tggcaactgc ataatggcaa aaagtttcgc
     9781 tgctgccggg tttttctcgg cccaggcttt gttggcaacg atatgcatgg tgctgaccgg
     9841 gaagccataa ttcgcaccat tcggcagttt ggtatcggcg tttttatcgc ccggcagtgc
     9901 ggagaacggc acctgcaacc agaccacatc tttccctggc ttcagctcat tactcaccca
     9961 gtacggcgtc caggtgtaat aaaacaccgg tttgccctct ttgtagcgac tgatggtgtc
    10021 ggccatcatc gctgcgtagt tcccctgatt atgcgtcacg gtgttggtca gttcatacgc
    10081 ggcaagctgg tggttgatcg caccttcgca gccccagcct gggttacagc cggttaaatc
    10141 cgctttaccg tcgccgttgg tatcgaacag tttggcgatc ttcggatctt tcagttgtgc
    10201 gatgttggtg attttgtact ggtcggcggt tttcttatcg atcaggtaac cctgtgccgc
    10261 gccgttaacg aatatccctt cacaataaaa tttcttatcg ccaccggcag cttcgtacat
    10321 gttgtcatgc agcggcgtcc agttcacggc ggtgaaggtt gcatcgccgg aagcaagtga
    10381 ggtgtagcca acgttgtaat ccacttcgct gggtttattg acggtataac ctaatttctc
    10441 cagtgcacga ctgaccagca gcgtctggaa ggtttcttca gtgatggtgc tctgaactgg
    10501 attaacagta atgcctttgc ccggcagatc ggctgcaaaa gtttgtgtag agataagcgt
    10561 ggcaaacgct gtcgcaaaaa gtacgctatg tcgcattgtt attccttttt ttggcagggt
    10621 gataatgccc ggcgttaccg ggcaagtgca gagttactta atgaatgggc gggtcagcag
    10681 accgacaggg ccagtggtgt accagcgacg gttgccgcga ctgcgtgagt cgcgcccaac
    10741 ggcctgcgtc aggcggtcga ggataatggc gaggatcaca atcccgacgc cgccaacggt
    10801 ggcaaggccc atatccagac gaccgatacc gcgaagtacc atctgaccca gcccgccgac
    10861 ggcaatcatc gaggcgatga ccaccataga aagagccagc atcagcgtct ggttaacgcc
    10921 cgccataatg gtcggcatcg ccagcggtaa ctgaactttg aacagcatct ggcgcgggct
    10981 ggcaccgaat gagcgcgagg cttcaatcag atccgccgga acctggttaa tccccagaat
    11041 cgtcagacgg ataatcggcg gcagagcaaa gatgatcgtc accactacgc ccggcacgtt
    11101 accgatacca aacagcatga cgattggtac cagataaaca aacgctggcg tggtctgcat
    11161 ggcatcaagc agtggacgaa taattttcgc cgctcgcgga cttctcgcca gccatatccc
    11221 caacggcaaa ccgatgacga tacagaacag cagggcggtt aacaccagcg ccagagtcac
    11281 cattgcctgc gaccaggcac cgattgcgcc gatggcaatc agcgaaacca gcgtcgccac
    11341 gcccattccg accccggaaa tctgccaggc gatgagagcg aaaacgataa tcgccactgg
    11401 cgctggcata cccagcagca attgctggaa actgttgagg atataatcaa ccggaacgcg
    11461 cacgccctgg aagacgggac ggaaatgggt aacaacccag tcgatccctt cagtgaccca
    11521 actgtcgagc gggatcagcg ttttatggaa cggatcgaga atattaaaat gctcgacgtt
    11581 tggcgcaggc gtactggtca gccagtcagc accaccgccg tcagtcggtg cagtcgtcgg
    11641 tgtaccccag gcgtctgcgg attgcgcggc actgtccgcc gctggcgtgg tatcccacgg
    11701 attattttga tcagccatta tttaccccct cacgatctaa agcgcgcagc agcattcctt
    11761 tcgaaatgat gccgacatac tgttggtcct cgtcgaccac gggcaccgca cagggtgcct
    11821 gtccgacatg agagagcaac tcgctaagag gcgtttgtgc atcgactgct aacggcgcat
    11881 caatcagcgc cgcatcaaga ccttgctgct gcgttaacgc ggttttaagc gaatcgatgg
    11941 agactgcgcc gacaaactta ttaccgcgtt cgataacgta gccatattcg cgatcttcat
    12001 cctgcaataa tttcagtgcc gaacgtgggc cgaagccagg ggttttacga attaagccat
    12061 tcggtgtccg gcgggcaata tctttcgcac tgaatacctg actaatatca acgccacgga
    12121 agaaggtacg gacataatca ttcgccggat tattgagaat ttcatccggt gtgccgacct
    12181 gtaccacttc accattttgc ataatggcaa ttcggtcgcc aatacgcatg gcttcatcga
    12241 gatcgtggga aataaagaca atggtgcgct gatgtttcgc ctgtaatttt accagctcat
    12301 cctgcatctc ggtgcgaatt aatggatcga gcgccgagaa ggcttcgtcc attaataaaa
    12361 tatccggatt aatcgctaac gcgcgggcta atcccacacg ttgacgcatc ccgccagaga
    12421 gttcatccgg gtagctgtgg gcataatttt ccagcccgac ctgacgcagt gcatcaaggg
    12481 ctttttcccg gcgttcttcg gcattaattc cggccaattc cataccgaac gcagtattgt
    12541 ccagcacggt catatgcggc attaaggcaa aggactggaa gaccatcgca atcttttttc
    12601 tgcgcacctc acggagtttg gcatcagata ttttggcaat atccacacca tcaatcagca
    12661 cttgcccgcg ggtgggttca atcaggcgat tgagaaggcg taccattgtg gatttacccg
    12721 agccggataa tcccatgatg acaaatatct cgccttcttc aatggccaga ctggcgtctt
    12781 ttacgccaag cgatagccca gttttttcca gaatttgttc ttttgaaagt ccttgttcga
    12841 tatatttgaa tgctcgctgt ggatgctcac caaatatttt ataaaggttt ttaatttcta
    12901 atttaattgc catgcaatag aaagattcct gttatttgtt tatgtcgata tattacccaa
    12961 tggaaatagt tacttttttc taccctaaca tactgagaat ctgaggcaac ccctgatggc
    13021 aaatgtggca tgaatattga tgcgagcaaa tcgccggaat ttcccgtgat ataagggctg
    13081 agagcaaatc gagaaaagtt gatatttttt gtcaaagata gcgaaaaaac gcctgaatat
    13141 tgtggtttca ggcgaataat gcaacagagt atcagtgtag atcaccacaa aatatttgcg
    13201 tgataaaaag ctatttggcg ggaattattt ccctgtcgcg ggtagtgata tttttgaaaa
    13261 taacaccctc agaaattcca gtcttcatct tctgtttcaa ccgctttccc catcacataa
    13321 gaggagcctg aaccggaaaa aaaatcgtga ttttcatcgg cattcggcga aagcgcggcg
    13381 aggattgccg gattcacttc cgccatttct gcgggaaata aaggttcgta gcccagattc
    13441 atcaaagcct tattggcgtt gtaacagaga aacgctttca catcgtcagc ccacggggtt
    13501 tcggcgtaca gctcatcggt gtattgcagc tcgttgtcgt agagttccag caacaaatcg
    13561 aaggcgaaac tcttcaactc ttcacgttgt cccagagata tcttttccat gtttttctga
    13621 tatttatagc ctatgtagta accgtggact gcttcatcac ggataatcag acggatcagg
    13681 tctgcggtat tggtcagctt tccgcggctg gaaaaataca tcggcagcca gaaaccggaa
    13741 tagaacaaaa aagattcaag aaacacactg gcgattttct ttttcagcgg atcatcaccg
    13801 cgataatgtt gctgaataat ctgagctttt cgctgcaacg gtgcgttttc ttcactccag
    13861 gcgtaggcgg catcgacatc tttggtctgg cacagcgtcg agaaaatcga actgtaagag
    13921 cgggcatgaa ccgcttccat aaagctgata ttcgataata ccgcttcttc atgaggcgtg
    13981 agtgcatcgg gcatcagcga aggcgcgccg ataacatttt gcagcgtgtc gagcagcgtc
    14041 aggccagtaa aaacgcgcat cgtcagttgt tgttctacga cagttaatgt ctgccaggca
    14101 ggaatatcgt tcgacagcgg caccttttct ggtagccaga aattgctggt caggcgattc
    14161 cacacctcca gatctttatc gtcagatatc ttgttccagt tgatggcgct gatacgtgag
    14221 agtttcatag atattcctta aagtgcacag gagacgcagc cttcaatttc agtgccttcc
    14281 agcgccatct gacgcaggcg aatgtaatag agcgttttga tacctttgcg ccaggcgtaa
    14341 atctgcgctt tgttgatatc gcgagtggtg gcggtatcgg ggaaaaacag cgtcagcgac
    14401 aaaccctgat cgacatggcg agtcgcttcc gcgtaggtgt cgatgatctt ttctgcgccg
    14461 atttcgtaag catcctgata cagcgccaga ttttcgttag tcataaacgg ggcagggtag
    14521 taaacgcgtc ctgttttgcc ctctttgcgt atctctactt tcgccacaat cggatgaata
    14581 ctcgacgtag catggttgat ataagagata gaaccggttg gcggcaccgc ctgaagattc
    14641 tggttgtaaa tgccgtagcg catcacgtcg tcgcgcagtt gcgcccacat ctcacgggta
    14701 ggtaacgtaa taccgctgcg ggcaaacagt tcgccaactt tcgccgtttt cggctgccag
    14761 ttaccttgca gatattggct aaaatattca ccactggcat aacgtgactg tttgaacccg
    14821 gcgaaggttt caccgcgttc gcgcgccagc aacatcgagg tacgcagtgc gtgccaggtg
    14881 atggcataga aatagagatt ggtgaaatcc agtgcttccg gcgaaccata agcgatgcct
    14941 tctcgcgcca gatagccgtg taaattcatc tgccccagtc cgatggcgtg tgaggcggca
    15001 tttccggctt cgatggacgg cacgctgcgg atatgactca tatctgacac cgccgttaaa
    15061 ccgcgcacgg cagtctctac cgtgcgggca aaatcggggg aatccatggt gtgcgcaata
    15121 ttcaacgaac ctaaattgca ggagatatca tggcctgtgc gggcatagtc gagattctcg
    15181 tcatactctg aggcacagtt gacctgcaaa atctctgagc agagattact catatttatg
    15241 cgcccggcga cagggttagc acggtttacc gtgtcttcat acatgatgta gggatagccg
    15301 gactcaaact ggatttctgc cagccgctgg aagaaatcac gggcgttgag gtattttttg
    15361 cggatgcgtt catcggcaac cagttcgtca tagtgttcgc tgatggcgat atcggcaaac
    15421 ggcttgccat aaactcgctc aacgtcatag ggcgaaaaca gcgccatctg cgcattctct
    15481 tttgccagat ggaaagtgat atccgggatc accacgccca gcgacagtgt tttaatgcgg
    15541 attttttcgt cggcattttc ccgttttgta tcgagaaaac gcagaatatc gggatgatga
    15601 gcatgtaaat agactgcacc tgccccctga cgcgcgccga gttggttggc ataggaaaat
    15661 gcgtcttcca gcattttcat caccggaatt acgccagaag attgattttc aatacgttta
    15721 attggcgcgc ccgcttctcg cagattcgac agcaaaaatg ccacgccgcc gccgcgtttc
    15781 gacagttgca gtgcggaatt taccgcccgg ccaatcgact ccatattgtc ttcaatacgc
    15841 agcagaaaac aggaaaccag ttcgccgcgc tgctgcttac cgcagttgag gaatgtcggc
    15901 gtggctggct gaaagcgtcc tgacagcatt tcattggtca gttgcaacgc cagcgtcgca
    15961 tcgccttgtg ccagcgtcag cgccaccatc gtgactcgat cggcaaaatc ttccagataa
    16021 cgtttaccgt cgaatgtctt caacgtatag ctggtgtaaa acttccatgc cccgaggaat
    16081 gtctggaaac gaaaaccgct ggtgtgtgcg tgggcaaaca gcgtaatgac aaagtcgcga
    16141 gagtagcgat taagaacgct ttcatcatag taaccttcgt tgaccagcca gttcaggcgc
    16201 tgctgctggc tactgaaggt cacactgttc ggacgcacat gcgtcgccat aaaggcgtca
    16261 acggcctggc ggtctttatc gaactgaatg cgacctgcgc tatcgtagag gttaagcatc
    16321 gcattcagcg cgtggtaatc catcgtttcc tgcgtcaggc attctgcggt tgtcgttgcc
    16381 aaaattcggt tactccttta cgaacgtttt cgatatcgct ttgcgtaccc atgagttcaa
    16441 aacggtacag ccacggcacg ccgcatttcc gggcaatcac atctccggcg cggccatacg
    16501 cctcaccaaa gttgcgatta ccagaagcaa taacgccgcg aagcaacgcc cggttgtgct
    16561 cgtcgtttaa aaagcgaatt acctatcgtg gcaccgcgcc agccgtaccg ccgccgccgt
    16621 aagagggcac gatcaggatg taaggctcgt ctacctgaat ccgttcccgc tcattgagcg
    16681 ggatgcgcac cgcgggcaga cctaaacgtt cgataaaacg ctgcgtgttt tcggagctgc
    16741 tggagaagta gacgagctgg ctcatgcact ggccgcgtgt ggcgctggat gcagacggtt
    16801 aatcatgtcc ggacggaaac cagaccagct aagatcgcca gcaatcacta ccggcaactg
    16861 acgaaagccc tgagcacgca acgcttccgc tgcttcagga acgcgatcga cattaatcat
    16921 ttcaaaatca aagccccggt tttccatcgc ccgtttggtg gcgtggcact gaacgcaatc
    16981 gttacgagtg taaatagtta tgcgcatgat tcgtatttcc gtttaaaatg aagatacggc
    17041 gcgatgatac gcgtcgggtt gtctctctgt tgatacagag atactagatg tagttgaaaa
    17101 aagattcaac cacacaatat atagcaaata gtgatcgaaa ttaccctaga tatgagcgtg
    17161 acgggggagg gggatttttg tgattcacca ggcaaaaaga aaccccgaag acaggcttcg
    17221 gggttaaaga cgcgtattta ttatcatttt tgcactacga tttgcgcatg cttaacagtg
    17281 cgccgataaa aatacctacc gcagctgctg tacccacgct acaccagggt ctttcacgaa
    17341 caaaagaatc cgcgcagcca acggcatcgc gtgcggcttg ctggacgcga gtacgaccat
    17401 gcattcgcgc cctggtttct ttcagcaatg cctgagcttt actgcgtgcg gcttcagctt
    17461 cccctttggc gtcgctgccc caggacttca atacagattc gaggctgtca gctaattgat
    17521 tgacatcatt ctggatatcc tgcacgccat catcaacgtc gttgcggttc ggtcggttaa
    17581 acatatgatc ccccattaga attcgatgta attttcagtg tagaccataa ttttcttaac
    17641 tgaggttcat cgcggcttta tggacttttc tgaaagcgtt gtgaatgcgc aatcaggtaa
    17701 ggtaggcccg cggttgataa taacgaggat caaatatgta tttacgacca gacgaggtgg
    17761 cgcgcgtact tgaaaaagtc ggttttactg tcgatgtggt aacccagaaa gcttatggtt
    17821 accgccgtgg ggaaaattat gtctatgtta atcgcgaagc gcgaatggga cgtaccgcac
    17881 tggtaattca tccaacatta aaagaacgta gttcgacact ggctgaaccc gcttccgata
    17941 ttaaaacttg cgatcattat caacaatttc cgctctattt agcaggcgag cgacacgagc
    18001 attacggtat cccgcatggc ttcagttcgc gtgttgcgct tgaacgttat ttgaatggtt
    18061 tatttggcga agccagttaa agacgcgact ggcgatgcca gtcgcgaaaa gaagagtcag
    18121 gcttttacct gctggtaatg gctgacttta aacagtcggc ggcaataatc gaggaagtag
    18181 ccataaaccg cccccatcaa catcgaaaca acgatgtttg aactgaccgc cgccataatc
    18241 tgatgccagt ctgcgcccac cactaacaag atcgccacat acaccggtga ctggaacgtc
    18301 acataagcca ggatatcagc cagatttttt atccagcccg acgggctaac tttgcgtgcc
    18361 gcgcgcataa atagatcgcg gtacataccg tatggccatg caattaagat gttcaccgga
    18421 atcgctacca atctggaata aaaagactgt tcgaagctca ttccggagag gaaaacttca
    18481 atacacatgt tcacgacaga acagtaaaca accatcgcga acgtatctgc aactgcatga
    18541 cgcaagcgtg actgcggtga gaacatgcta atgctcctta ataaaagcgt aatgggatgt
    18601 taatggagat gaaagcagtg gatctcattg ccatctgcat tttcgcatag cgtatatcgc
    18661 tggctatgac tattcaatta gtgaaaaatt tttatttagt cagtttttgt agataaaata
    18721 ttccgtaacg gctcgttttt tgtacatt
//
LOCUS       DAZOZD010000067        18523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOZD010000067 DAZOZD010000000
VERSION     DAZOZD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.067"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHUPB_001876"
     CDS             <1..1710
                     /locus_tag="ACHUPB_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR0758702.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHUPB_001877"
     CDS             1711..3360
                     /locus_tag="ACHUPB_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0758703.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHUPB_001878"
     CDS             3365..4141
                     /locus_tag="ACHUPB_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0758704.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUPB_001879"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUPB_001879"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR0758705.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHUPB_001880"
     CDS             complement(5430..6752)
                     /locus_tag="ACHUPB_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR0758706.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUPB_001881"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUPB_001881"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR0758707.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUPB_001882"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUPB_001882"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR0758708.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUPB_001883"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUPB_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR0758709.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUPB_001884"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="ACHUPB_001884"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR0758710.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUPB_001885"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="ACHUPB_001885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0758711.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUPB_001886"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="ACHUPB_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0758712.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUPB_001887"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="ACHUPB_001887"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0758713.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAZOZD010000068        18005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOZD010000068 DAZOZD010000000
VERSION     DAZOZD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.068"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            227..532
                     /locus_tag="ACHUPB_001888"
     CDS             227..532
                     /locus_tag="ACHUPB_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758714.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            639..1283
                     /gene="gfcB"
                     /locus_tag="ACHUPB_001889"
     CDS             639..1283
                     /gene="gfcB"
                     /locus_tag="ACHUPB_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0758715.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1280..2026
                     /locus_tag="ACHUPB_001890"
     CDS             1280..2026
                     /locus_tag="ACHUPB_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0758716.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2026..4122
                     /locus_tag="ACHUPB_001891"
     CDS             2026..4122
                     /locus_tag="ACHUPB_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0758717.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4168..5307
                     /locus_tag="ACHUPB_001892"
     CDS             4168..5307
                     /locus_tag="ACHUPB_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0758718.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5295..5741
                     /gene="etp"
                     /locus_tag="ACHUPB_001893"
                     /gene_synonym="yccY"
     CDS             5295..5741
                     /gene="etp"
                     /locus_tag="ACHUPB_001893"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0758719.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5761..7941
                     /gene="etk"
                     /locus_tag="ACHUPB_001894"
     CDS             5761..7941
                     /gene="etk"
                     /locus_tag="ACHUPB_001894"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR0758720.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8056..9354)
                     /gene="appA"
                     /locus_tag="ACHUPB_001895"
     CDS             complement(8056..9354)
                     /gene="appA"
                     /locus_tag="ACHUPB_001895"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0758721.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9434..9526)
                     /gene="cbdX"
                     /locus_tag="ACHUPB_001896"
     CDS             complement(9434..9526)
                     /gene="cbdX"
                     /locus_tag="ACHUPB_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0758722.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9539..10675)
                     /gene="appB"
                     /locus_tag="ACHUPB_001897"
     CDS             complement(9539..10675)
                     /gene="appB"
                     /locus_tag="ACHUPB_001897"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0758723.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10687..12231)
                     /gene="appC"
                     /locus_tag="ACHUPB_001898"
     CDS             complement(10687..12231)
                     /gene="appC"
                     /locus_tag="ACHUPB_001898"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0758724.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12365..13222)
                     /locus_tag="ACHUPB_001899"
     CDS             complement(12365..13222)
                     /locus_tag="ACHUPB_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0758725.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13279..13617)
                     /gene="hyaE"
                     /locus_tag="ACHUPB_001900"
     CDS             complement(13279..13617)
                     /gene="hyaE"
                     /locus_tag="ACHUPB_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0758726.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(13614..14201)
                     /gene="hyaD"
                     /locus_tag="ACHUPB_001901"
     CDS             complement(13614..14201)
                     /gene="hyaD"
                     /locus_tag="ACHUPB_001901"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0758727.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14198..14904)
                     /gene="hyaC"
                     /locus_tag="ACHUPB_001902"
                     /pseudo
     CDS             complement(14198..14904)
                     /gene="hyaC"
                     /locus_tag="ACHUPB_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(14923..16716)
                     /gene="hyaB"
                     /locus_tag="ACHUPB_001903"
     CDS             complement(14923..16716)
                     /gene="hyaB"
                     /locus_tag="ACHUPB_001903"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0758728.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16713..17831)
                     /gene="hyaA"
                     /locus_tag="ACHUPB_001904"
     CDS             complement(16713..17831)
                     /gene="hyaA"
                     /locus_tag="ACHUPB_001904"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0758729.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4390 a         4804 c         4693 g         4118 t
ORIGIN      
        1 tttaatttgt attttgctaa gatttatctt atgctcacaa gtcgtatttc cagaggggat
       61 cgttaataat cttatatttc cctctgcgca cctttcctac ccgcagtatc agtatatagt
      121 gcaagcggta tgaggaaagg gagatttcac aaggatgtta tactcaaagt agacaatcct
      181 gatctcaagc gtacgtattg tccatgcgat gaataaagga aaagttatga aacacaagct
      241 ttctgcaatc cttatggcct tcatgttgac gacgccagct gcgtttgctg cctctgaagc
      301 aacaaacggg accgaggcaa cgacgggtac tactggcacc acaacgacca ctaccggcgc
      361 aaccacgact gctactacca ctggtggcgt ggctgctggc gctgtcggca cggcgacagt
      421 tgtcggtgtg gcaacggctg ttggtgtggc gacgttggca gttgttgcag caaatgacag
      481 tggcgatggc ggttctcata acacctcaac caccacgtcc actacacgct aatactgctt
      541 taacgcgtcg aaattaaagt taaatgatac ccggttgcga tattcgtacc gggctttaga
      601 gatttgcatt tttttaatta acctttcaag gattaaaggt gcgccctctt attttatcga
      661 ttttcgcact atttcttgcg ggatgtacgc acagccagca aagtatggtc gatacatttc
      721 gcgccagcct tttcgataat caggatatca ccgtagcgga tcagcagatc caggcgttgc
      781 cttattccac gatgtattta cgccttaatg aagggcaacg aatctttgtg gtactgggat
      841 atatagaaca agaacaaagc aaatggttat cccaggataa cgccatgctg gttacccaca
      901 atggacgtct tttaaaaacc gtcaaactta ataataatct gctggaagtg acaaattccg
      961 ggcaggaccc tctgcgtaac gcgctggcaa taaaagatgg cagccgctgg acgcgcgata
     1021 ttctctggag tgaagacaac cattttcgct ctgcgaccct gagttctact ttttcctttg
     1081 ctggattaga gacgctgaat attgcgggtc gcaatgtgct gtgtaatgtc tggcaggaag
     1141 aggtgacttc cacgcggcca gaaaaacagt ggcaaaacac attctgggtc gattcggcta
     1201 ctggccaggt tcgtcaaagt cggcaaatgt taggcgcagg ggttattccc gtagaaatga
     1261 cgtttcttaa acccgcacca tgaataaatt acagtcgtat ttcattgcca gcgtacttta
     1321 cgtaatgaca ccccatgcct ttgcgcaagg aacggtgact atttatctgc ctggcgaaca
     1381 acagacactt tccgttggcc ccatggaaaa tgttgtccag ttggtgacac aaccgcaact
     1441 gcgcgatcgc ctctggtggc ctggtgcctt gctgaccgac tctgcggcga aagctaaagc
     1501 gcttaaagac taccaacatg tcatggcaca gcttgcttcc tgggaagcag aagctgacga
     1561 cgatgtggcg gcaacgatta aatccgtgcg ccagcagtta ctcaatctca atatcactgg
     1621 tcgcttaccg gtcaaactgg accctgattt tgtacgagtg gatgagaaca gcaatccccc
     1681 gttggtcggg gactatacgc tctataccgt acagcgtccg gtgactatta ctctgctggg
     1741 tgcggtttct ggggccgggc aattgccctg gcaggcaggc cgtagcgtta ccgattatct
     1801 gcaagatcac ccccgccttg caggcgcgga caagaataac gttatggtga ttacgcctga
     1861 gggtgagact gtcgtcgccc ctgttgccct gtggaataaa agacatgtgg aaccaccgcc
     1921 tggcagccag ctttggttgg gcttttctgc gcatgtactg cccgaaaaat atgctgattt
     1981 aaatgaccag attgtgtctg ttttaacgca acgtgttcct gactgatgaa gaagaattct
     2041 tatcttttaa gctgcctggc cattgccgtc tccagtgcct gtcatgctga agtattaacc
     2101 tacccggatc cgctgggttc gtcgcaatca gactttggcg gcacaggatt gttgcagatg
     2161 ccaaatgcgc gcatcgcacc ggaaggtgaa ttcagcgtca actaccggga taacgatcaa
     2221 taccggttct actccacctc cgtggcgctg ttcccatggc tggaaggcac cattcgttat
     2281 acggatgtgc gcacacgcaa atatagccag tgggaagatt tcagcggcga tcagtcatac
     2341 aaagacaaat cattcgattt taaacttcgc ctgtgggaag aaggttactg gctaccgcaa
     2401 gtggcgtttg gtaaacgtga tattgctggt acgggtctgt ttgacggtga gtatctggtg
     2461 gccagcaagc aagcggggcc atttgatttc accctcggga tggcatgggg ctacgccggt
     2521 aatgcgggca atattaccaa cccgttttgc cgggtgagcg ataaatattg tcatcgcgca
     2581 gagtctcacg atgcgggcga tatcagcttt agcgatatct ttcgtggccc ggcttccatc
     2641 tttggcggca ttgagtatca aacgccgtgg aatcccctgc gtctgaaact cgaatacgat
     2701 ggcaacaatt accagaatga tttcgctggc aaactgcctc aggcaagcca tttcaacgtc
     2761 ggcgcagttt atcgcgctgc cagctgggca gatctcaacc tgagttatga acgcggtaac
     2821 acgttgatgt ttggcttcac gttacggacc aatttcaacg atctgcgccc tgccctgcgc
     2881 gatacgccaa aaccggcata tcaacctgcg cctgaatctg aaggattgca gtacaccaca
     2941 gtagcgaacc aacttaccgc gctgaagtac aacgcaggtt ttgaagcacc ggaaattcag
     3001 ctgcgcgata agacgctgta tatgtctggt caacaataca aataccgtga ttctcgcgaa
     3061 gcggtcgatc gtgccaaccg gattctggtg aataacctgc cgcaaggcgt tgagaagatt
     3121 agcgtgacgc aaaagcgcga gcatatggcg atggtgacta ccgaaaccga cgtagccagc
     3181 ctgcgcaaac agctggctgg tacagcgcct ggtcaatcag agcaacttca acaacaacgt
     3241 gttgaagcag aagatctttc tgcctttggt cggggctacc gtattcgtga agatcgcttt
     3301 agctactctt tcaacccaac actttcacag tcgctgggcg gcccggaaga tttctatatg
     3361 ttccagctgg ggctgatgtc cagtgcccgc tactggttta ccgaccacct gctgcttgat
     3421 ggcggtattt tcaccaatat ttacaacaac tacgacaagt ttaagtcttc gctgttgccc
     3481 gcggactcta ccctgccccg cgtgcgcacg catatccgtg attacgttcg caatgacgtt
     3541 tatctcaaca acttgcaggc gaactacttt gccgacttag gcaatggttt ctatggccag
     3601 gtgtatggcg gttatctgga aacgatgtac gccggtgtcg gttccgagct gctttatcgc
     3661 ccgctagatg ccagctgggc gctgggtgtg gacgttaact acgtgaagca acgtgactgg
     3721 gacaacatga tgcgcttcac cgattattcc acgccaactg gtttcgtgac ggcttactgg
     3781 aacccgccga cgctcaatgg cgtactgatg aaacttagcg ttgggcaata tctggcaaaa
     3841 gataaagggg caacgatcga cgtcgccaaa cgctttgaca gcggcgtggc ggtaggggta
     3901 tgggcggcaa tcagtaacgt atctaaagat gactacggcg aaggcggctt tagtaaaggt
     3961 ttttatatct cgattccgtt cgacttgatg accattggac ctaaccgcaa ccgcgcggtg
     4021 gtttcgtgga caccattgac gcgtgatggt ggacaaatgc tgtcacgcaa ataccagctc
     4081 tatccaatga cggcagagcg agaagtaccg gttggacaat aacctgacgt aattcataac
     4141 attacattga ctgataaata taaaccaatg aaaaagaaca tttttaagtt ttcggtactg
     4201 acattggctg ttctgtcttt gacagcatgt acccttgttc ctggtcagaa tttatcgacc
     4261 agtaacaagg atgtcattga actacctgac aatcagtatg acctggacaa aatggtcaat
     4321 atctatcccg tcacgccagg attgattgat cagttgcgcg ccaaaccgat tatgtcgcag
     4381 gctaacccgg agctggagca gcaaatcgca aactatgaat atcgtatcgg gattggtgat
     4441 gtgttgatgg ttacggtctg ggatcacccg gaactaacca cccccgcagg gcaataccgt
     4501 agcgccagtg ataccggcaa ctgggttaac gccgatggtg cgattttcta cccctatatt
     4561 ggtcgcctga aagtggccgg gaaaacactt acccaggtac gcaacgagat tacagcgcgt
     4621 ctcgattcag tcattgaaag cccgcaagtc gatgtcagcg tcgcggcgtt ccgatcgcaa
     4681 aaagcgtacg tcaccggcga agtcagcaaa tcgggtcagc agccgattac caatattcct
     4741 ctgaccatca tggatgcgat taacgccgcg ggcggtctga ccgcagatgc agactggcgc
     4801 aacgtggtgc tcacccagaa tggcgtaaag accaaagtga acctctatgc gctgatgcag
     4861 cgtggcgact tgcgccagaa caaactgctg cacccgggcg atattttatt tattccgcgc
     4921 aacgatgatc tgaaagtctt cgtgatgggt gaagtgggta aacagagcac gctgaaaatg
     4981 gaccgcagcg gtatgaccct ggcggaagcg ctgggtaacg ctgagggtat gaatcaggat
     5041 gtcgctgatg cgacggggat ttttgttatt cgtgcaacgc aaaacaagca gaatggcaaa
     5101 atcgccaata tttatcagct gaatgcgaag gacgcatcgg caatgatact cggaacggaa
     5161 ttccagcttg aaccttacga cattgtttat gtcactaccg cgccgctggc tcgttggaac
     5221 cgtgtgattt ctctgctggt accgacgatt tccggggtgc atgacctgac agagacttca
     5281 cggtggattc aaacatggcc caactaaaat ttaactcaat cctggtggtt tgtaccggca
     5341 acatttgccg ttcgcccatt ggcgagcgct tgctgcgtaa acgtctgccg ggcgtaaaag
     5401 tcaaatcggc aggtgttcat ggtctgataa aacaccctgc agatgcgaca gcggcagatg
     5461 ttgccgctaa tcatggcgta tctcttgaag gacatgccgg acgtaagctc actgctgaga
     5521 tggcacgaaa ttacgatctg attctggcaa tggagtcgga acatattgct caggttaccg
     5581 caattgctcc cgaggtccgc gggaagacaa tgctgtttgg gcaatggctg gaacagaaag
     5641 agatcccgga tccctatcgt aaaagtcagg acgcatttga acatgtctac ggtatgttgg
     5701 agcgcgccag tcaggaatgg gcgaagcgcc tcagccgata atttgagttt ataaaatacg
     5761 atgacaacta aaaatatgaa tacgccacca ggcagcactc aggaaaatga gatcgatctg
     5821 cttcgtctgg tcggcgagtt atgggatcac cgtaagttta ttatcagcgt gaccgcgtta
     5881 ttcacgctga tcgctgtcgc ttactcgctg ttaagcacac caatttatca ggcagatact
     5941 ctggtccagg ttgagcaaaa acagggcaac gccattctca gcggcctgag cgatatgatc
     6001 cctaactcat cgcccgagtc tgcaccggag atccaactgc tgcaatcgcg catgattctc
     6061 ggtaaaacca ttgctgaact gaatctgcgc gacatagttg agcagaagta ttttccgatt
     6121 gtgggtcgcg gctgggcgag attaaccaaa gaaaaaccag gtgagctggc gatcagctgg
     6181 atgcatattc cacaactgaa tggtcaggat cagcaactga cactcacggt tggggaaaac
     6241 ggccactata cactggaagg tgaagagttc accgtcaatg gtatggtcgg ccagcgtctg
     6301 gaaaaagatg gcgttgcgct gactatcgcg gacattaagg ccaaaccagg aacacagttt
     6361 gtcctgagcc agcgtaccga actggaagcg attaacgcat tgcaggaaac ctttaccgtt
     6421 agcgaacgca gtaaagaaag cgggatgctg gaacttacca tgactggtga tgatccccag
     6481 ttgattactc gtattctgaa cagcatcgct aacaactatt tgcaacagaa tatcgctcgc
     6541 caggcggcgc aggattcaca aagccttgaa ttcttacagc gccagttacc tgaagtgcgc
     6601 agcgagctgg accaggcgga agaaaaactc aacgtttatc gccagcagcg cgattcggtt
     6661 gaccttaacc tggaagccaa agccgttctt gagcagattg tgaacgttga taatcaactc
     6721 aatgagctga ccttccgcga ggcagagatc tcccagctgt ataagaaaga tcacccaact
     6781 tatcgtgcgc tgctggaaaa acgccagacg ctggagcaag aacgcaaacg cctgaataag
     6841 cgggtatcgg caatgccttc cacccaacag gaagtgttgc gtttaagtcg tgacgtagaa
     6901 gcgggccgtg cggtatatct gcaattactt aaccgccagc aggagttgag tatttcgaaa
     6961 tccagtgcca ttggtaacgt gcggattatc gacccggcag tcactcagcc gcagccagtg
     7021 aaaccgaaaa aagcgttgaa tgtggtgctt ggttttattc ttggcctgtt tatttctgtg
     7081 ggtgccgtgc tggcgcgtgc gatgttgcgt cgtggtgtag aagccccgga acaactggaa
     7141 gagcacggca tcagcgttta tgccactatc ccaatgtccg agtggctgga taaacgcacc
     7201 cgtctgcgta agaaaaattt attttctaat cagcagcgcc atcgtactaa aaatatcccc
     7261 ttcctggcgg tggataaccc ggcggattct gctgtggaag ccgtacgtgc gctacgaacc
     7321 agtctgcatt tcgctatgat ggagacggag aataacattc tgatgatcac cggtgcgacg
     7381 ccagacagtg gtaaaacgtt tgtcagttca actctggcag cggtgatcgc ccagtccgat
     7441 caaaaagtgt tatttattga tgccgactta cgccgtggtt attcgcataa cctgtttacc
     7501 gtgagtaatg aacatggctt gtcggaatat ctggcaggta aagatgagct caacaaagtg
     7561 atccagcatt ttggcaaagg aggctttgat gtgattactc gcggtcaggt gccacctaac
     7621 ccgtctgaac tgctgatgcg cgatcggatg cgtcaattac tggaatgggc gaacgaccat
     7681 tacgatctgg tgattgtcga tacgccgccg atgctggcgg tgagtgatgc cgcggtcgtg
     7741 gggcgttctg ttggcaccag cctgctggtt gcgcgttttg gcttgaacac cgccaaagag
     7801 gtgagtttgt caatgcagcg tctggaacag gcaggcgtca atattaaagg cgctatcctc
     7861 aatggtgtga ttaaacgcgc cagcaccgct tacagttacg gctataacta ttacggttat
     7921 agttactccg agaaagagta aaactgaaag tggccggatg cggcgtgaac gccttttccg
     7981 gtctacgcat tagacagttc ttcgtttgtc atcagcctca gagcattcag gtaactgaat
     8041 gctctttttt atgcattaca aactgcacgc cggtatgcgt gcttcattca cgatttgcgt
     8101 aaaacctgcc aacgaacaca tgccctgcgc atttcgctct tcacatcctg ccagggtcag
     8161 tttcacctct ccgggcggcg tatttaatga cagcggcgtt ttatcacgca tctgctgtaa
     8221 agtctggaag accagcgaaa cctgaatcca ctggctgtta tcgcttagcc gacgccagcg
     8281 ttcaaacacc agttcaccac ctggcggcgt gttatccggc tgaccgggaa gcgtccagtt
     8341 gagctccagt gcgccgccga gatttgccag attagtatcg tgtccggcga taaacagcac
     8401 tgaagtgggt aatgtcacac catacgcctg tttttgcggt ggatggggcg tcaacgctgt
     8461 cttgatcaaa tctaataacg gggtggcgcg gctgcgggca acctctggcg tgcgttgtag
     8521 caaataaaat tgcgcgttat gcaaacttag caaggtgttc cactggtgtg aatcggtgat
     8581 ccttccccac cccggctccg gcattccctg tgcttgttgc aggagaaata tctccgtcag
     8641 cattgatgcg aggcttaccg caccggttaa tgagacattg tcggcgctca ccttgagttc
     8701 cgatggtaat gcctgcgtta atgaacagct ttcgtcctgt ttctcacgtt taaggcacaa
     8761 gtttgattgc ggaaaattaa gcacccgttc cagttcgcga aacgccgttt gccgatgccc
     8821 ggtaaagtca gcaattgacc ctcctgccct gctgaggatc gcgtcagtca cgttcgcgtt
     8881 atccagttgg caaacgccag tttttagagg attaaataac ggatcgggac tggacgtatc
     8941 agcctgggta tgtacggtta ttgcacagtc aggtgccagc ccggcggcga aggcttcgcc
     9001 tgttttacgg gtacgctcgt cgacatcagc aataatcgcg acctgaccag actgcgggca
     9061 gccctttttc gccagcaatc cgtcggctac cagacgctgg cgttggtaat gtccgagata
     9121 ggcgattagc tcaccaccgc gcggtgtcag ccaacccagt tttaccggcc aggttggcca
     9181 tgcgtctggg gtgacatcct gcatcagttg cgtggccttg gttggagcac gcacaccatg
     9241 acgactgaca atcaccacac tttccagctt cagctccggc tcactctgag cgaatgcaga
     9301 ttgcggggtt aacggaatca gaagagataa aaatgggatt aagatcgctt tcatcgatat
     9361 gtttccgctt ttcatatctg acattattga ttgcctgatg cgatgctaat gcgccgcatc
     9421 aggcctacgt tcgttaactt ttcagacgcg ggtccagcca taccaacaca agggtggaga
     9481 gcgaacacat caacaaaatg ccgacgaacc aaagtaaata ccacattgtt cctgctcctt
     9541 agtacaactc gttttcgtta cggcggagag tttctgttgt catgcgtccc cacattttgt
     9601 agtagctcca gagagtgtag agcaacacaa tgggcaaaaa tatcagcacg attaccaaca
     9661 taatgctcag cgtcagctga ctggaagtgc tgtcccacaa cgtcaggctg gagattggac
     9721 tcacgcttga cggcatgaca aaggggaaca gcgtgatgcc tgccgtgaaa atcacgccaa
     9781 attgcatcaa tgacgccatt aaaaatcccc aacccgggcg accacgataa atcgccatca
     9841 ccgtcagcaa tgggcagaag aaccccagca gcgggaagat ccacaaaacg ggcgattcga
     9901 cgaaattatt catccaggca ccaggtagca ctgccaccag tttcattaac ggattggaag
     9961 gaccgttagc atcctgggcg agcagtacaa agccatcaat accgacccac agccagtaac
    10021 ccgccagcaa aaagcacagc atcaccaaca gtgcagcgcg tttggtggcc agctgtgaac
    10081 gcagatgaat cacaccaaca gttttcagtt gtaaccagac gccaccttgc aaaatcacca
    10141 tcccaaggct gagcaatccg cacaataaag ggaatggcgt cagcagttgc cagaagctgc
    10201 cgagatactc cacgcgtaat tgcggtgtga aggcaaacgg cacgccgagc aacaagttgc
    10261 cgaaggcgat accgaagact accggcggca ccagactgcc gatgaccaga ccggcgtccc
    10321 acattttacg ccagcgtgca tcggcgattt ttccgcgata atcaaaggcc agcgggcgga
    10381 agaacagtga gcacagcacc aggatcatcg ccacataaaa gccggaaaac gccgctgcat
    10441 acactctggg ccaggcggca aataatgccc caccagcgag gatcaaccag acctggttgc
    10501 cttcccagtg tgcaccaacg ctgtttatca ctatccggcg ttcatcatca ttacgcgcca
    10561 ccagcggcag cagacagccg atccccatgt caaatccgtc ggagatcata aagaccacca
    10621 ggatcacgcc aatcagcagc caccagatga agcgcaatgt ttcataatca aacatgattt
    10681 tcccctttac ccctgttgct gcgtcggttg ttcactctgc atcgcgctcg gccccagacg
    10741 ggcatatttc tgcatcaggt agacttcggc gattaagaac agggtgtaaa gccctacgat
    10801 catgatcagt gagaaagcca gttgtcctgt ggttaaagcg gagtgcgcgg agtatgtcgg
    10861 taagatgtcc tgtatcgccc acggctgacg accaaactcg gtcataaacc acccggcttc
    10921 aatcgcaatc cacggcaacg gcaaactcca gagcgccatt ttcagcaccc agcgatgctg
    10981 gtcgattttg ccacgcagcg tctggacaag cgcaatcagc atcaccagta gcggcaggga
    11041 accacagccc accatgatgc ggaaactcca gaataccggc gcaacctgag gtatcgcgcc
    11101 acgcatcgcc gcctggtact gtgcggctgt gacatgattc atatccggcg catagcggga
    11161 gagcaacatg ccgtagccca ggtcaccttc cagttcccgg aatgcctgca acacatgcgg
    11221 ctcacgattg ccttgcgata tttcctgcat taacagccag gccatacgtc cgcgttgcaa
    11281 gcgtgggtag gtttcagcca tcaaattttt cagacccggc acggttttat ctaatgagtg
    11341 agtggcgagg atccctaaca gcgcgggaat tttgatggca aaggcgttac gctcttgatc
    11401 ctgttccggc caggcaacca catggaacgg tgcaggagca ggttccgttt gccactcccc
    11461 ttccatcgcc gccagtttta ccggttgtac ttgcgcgact tcatacgcag aactgtctcc
    11521 gagttgtagc gtgccgataa tcgccagagt accgaagacg gaaccgatgg caaacgagcg
    11581 taatgcaaca tcgcgctccc gtccgcgcag taaataccag gcgctgatcg ccataataaa
    11641 catggccccg gtcacgtagc ccgccattac ggtgtgcaca aatttcacct ggctgaccgg
    11701 gttaaagacc agctcgctga agctggtcat ctccatacgt agggtgtcga tatcaaaatg
    11761 cgcaccggtc gggtattgca tccaaccgtt ggcattcaat atccacaatg cagagagatt
    11821 tgaaccgaac gccaccagcc acgtcaccag caggtgctgg tatttattca gccgctgcca
    11881 gccgaagaag aaaagcccga caaaggtgga ttcgaggaag aaggccatta atgcttccat
    11941 cgccagcggt gcgccaaaaa tatcgcccac atagttggaa tagaatgacc agttagtacc
    12001 aaactgaaac tccatggtca ggccggtagc cacgccaaga gcaaagttga taccgaagag
    12061 cttaccccag aagcgcgtca tatcgcggta gattgttttg ccggtgacca cgtaaatggt
    12121 ttccatgata gccagcaaaa aaatcagccc cagggtaagg ggtacaaata aaaagtgata
    12181 cagcgcggtc agagcaaact gccagcgcga taaatcaatg acatcccaca tgcgcactcc
    12241 tgtaggcgaa gagagaaaaa taacccgata atgttagatc tccctcttta tcctgaaact
    12301 ctacgaccag aggtattcgc cgattgcgat attcataaga ctgaaagcga tacttaccgt
    12361 ctttttacgt cggtgcagct tccgccagcc actgacatac ctcgctaagc cgctgcgcag
    12421 agtcgaccaa atcttctggc gctgccagca ccacttccgg tattgggcag atttcataac
    12481 tttccagtaa aggacctttt aaggtgtccg tacagcgtaa atgccagaca tggcgtaacc
    12541 ccgtggcgtt gatatagctc tcgccatagc caatggtacg aatctgaata tttcccggcc
    12601 cacagagacg tgagagaaaa agccgatcag cctcgctgat gggtaattgc gtcagattaa
    12661 tgctgtgcgg ctgcgcgtcg gggttacgta catgcgccag taactcatgc gccaacggta
    12721 ggccattcat caggccatca atgggcggcg gtaacagcga atctgtcggc aagagatttt
    12781 gcgtggccgc ctgccacaac gccagcggcg cgcagccggc ctccagtttg tcctccagca
    12841 acttttcgcc gcgacgtttg cgcacccgcc ataatccgca gaagatcgcc tcctgtattt
    12901 cactttcgct gtcgtcagcc tgctgaatgc gcactgacac ttccccttcc ccgagaagcg
    12961 tattgataaa gtgacgatcg tcggcgttga gatttaacag atcgactgcc agcacctcgc
    13021 ccaacggcgg ctggcgttga cttagttgat gttgtaactc gtttaacagc gcaatcacct
    13081 gcgggctgtg agcacattgc tccagggccg ccatactcgg ttcatcattc acctgacagc
    13141 tgatcggcag tggattcatg ctgaaactgt catcgttcgg ttgcgttcct ggccccagca
    13201 gatggaaaaa agtttcgctc atgaggcacg ctcctgttgt ggttcgacaa gcccacgcat
    13261 caggtttatc agttccgctc acgggtgaat accattcagc acaccgcgat agtttccgcc
    13321 ggtaaacact aatgtggcgg gaaagcgaaa gacgccaaag cgatctccga tggcttcgct
    13381 ctgttcaagg tcagcaatcg ccacctgcca tgtgtagccg ggaaactcgc gcagtaattc
    13441 gccaatcatt accggattat cgctgacctc tggcgtgcgt ttcgggtcac tgcttaataa
    13501 caccacgccg tctggcgctt gcgtaagcca gtcgtcaaga cggaattcac taaccggcgt
    13561 ccagccgcgc gccagcattc gttgccacaa cgcatcaaat ggcgtgtcgt tgctcatccc
    13621 tgctcctcca gtgtcatcct gtactggcgc aagcgaacgc cttcgtaatt ttccatcgac
    13681 agacagtcat aattgagaca gcgcgattca ttagccggtt gcggcacaat tccccatgca
    13741 gccagctgcg ccagcgccgc ctgttccgct gcgggcagtt gctcccgtgc cagttcgctc
    13801 aggctgccgc cgtagtcgtc gagcattgcg ggttgcagac cgacgagggc aatatgtgct
    13861 ggcagatgtc cgcggatatc tgccagcgcc agcacttcgg agaaactgtt ctggtgcagg
    13921 ctcattttct tcgcgctgag ataagccgga atacgttctc cggcataggt tcgcagcgtt
    13981 ccaggttcca gcccgtagtc aatggcatcg agaatcaaca gatggctggc gctttcgaca
    14041 taccccagca agttcagtcc ctgagtaccg ccatcgacaa tctccacata ctcgggccag
    14101 tggtaatggg catacaaccg ttccgccacc cgcacgccga aaccttcatc ggcccacagc
    14161 aggttgccca gccccattac cacaacgcgt tgctcgctca tgaacgctcc ttgttactta
    14221 ttttgccaaa tttgtggcta cggtagccgt tgaccatggt ggagatcacc gtgtcgtcgg
    14281 acatgatgtc ttcacgcagc gccatataga catgaccgat cacaaacgcg ccaatcagcc
    14341 acatccccag ccgatgccag ctgtgaatgt ccattgagtt gcctcccgtc cagtagaaaa
    14401 attccaccac ataacggaac ggcgcaaaaa tagcgtactg gctgtgttcg ctgtacagcg
    14461 caaagccggt gatgatcata aagaccgaca tcaggaaata gccgaacatc gccgcctggg
    14521 cgatggggtt atggcctata tcggcactcg gacgttttgc cagaacagat accagcggat
    14581 ttcataccac acgccctgcc accagctttt acgccatacc ggcacgataa acagctcgcg
    14641 ggagtatcga ttgccaacaa aagcccagta gatccgcatc agcaagacca cggtaaaaac
    14701 catcccggcg ctgaagtgaa ttaacctgat gtagcccata tagaacagat acgtcgcctc
    14761 gccgctgacg gaaggtagcg gcttgccgat aaagtatccg gtgaccatca acaccgccat
    14821 gcataacacc gtcagccagt gccagatgcg gactggcgct tcaaagacat agtggctgac
    14881 aacgttgtcg cttttctgtt gcatgatgat tctccttcgc tgttaacgca cctgcacgga
    14941 gatcagctcg ctaccgtcgt cgcccagcac gtgtgttgaa caggcgaggc acgggtcaaa
    15001 gctgtgcaga gtacgcagga tctccagcgg ttgctcgggg atcgccattt tggtgttcat
    15061 cagcgccgct tcataagcgc caatctgccc tttgggatcg cgcgggctgg tgttccaggt
    15121 ggtcggcacc acgcactggt agagatcaat cttgccatcg cgaatggcgg cccagtggcc
    15181 taacgccccg cgcggcgctt cggtaaagcc gacgccacgg cactctgtcg gccaggttgc
    15241 aggttcccat ttttccgtgg aggcggtggc aagattgccg tttttcaggt tagtcatcag
    15301 cttgttgaag aaatattgca acttacctgc ggcccactgt gcttcgtgcg cgcggcacaa
    15361 aatgcggccc aacgttgact ggataccgga aagcggcagg ttcagcgccg acatcatgcg
    15421 atcgaccgac tcaacggtcg cagcatcgcc tttgtgataa gcgattaacg tgcgtgccag
    15481 cggccccact tccatcgcgt taccgcgcca gcgcggcgct ttgatccacg agtagcgttc
    15541 ctgttcattc agctgctgaa tgttggtatc gctgcctttg acatcgccgg ggttgtacca
    15601 cgggtcggta atgccatcga atggatgacg cccgacctga tcgttgggat aacgatacca
    15661 ggcgtggtcg acaaactcct gcacctgctg cggatcaacc aaatccactg gcagcacatt
    15721 gttgaagtcg ccgttaatca ccgcgccgcc aggcatcagc agacttttct caccaaagtc
    15781 gttggcaata tccgggaacg caccgtagct gaggacacat ttatcagaaa gacccgtgcc
    15841 gatttcgctc cacggtttgt tgaactgacc gatggctaag gcgtcgggga tcatcacgtt
    15901 gttaatgaag tccgccgtgc gggtgataat cgactgcacc aggttcaggc gttccatatt
    15961 gaccgccccg accgcgccgc tttcgtcaat gttaatggcg caaggcatcc cgccgacaat
    16021 ccagtttgga tgcgggtttt taccgccaaa gaccgcgtgg attttgacaa tttcacgctg
    16081 gaaatcgaga gcttcgagat agtgggcaaa gcccatcagg ttagcttctg gcggcagttt
    16141 gtactgcggg tgcccccagt agccattgcg gaagatcccc aactgcccgc cttcaacaaa
    16201 ttttttcagg cggttttgta cgtcgaagaa atagccaggg gatgatttcg gccaagagga
    16261 gagactttgc gccagttcgg aggttttccg cgggtcggct ttcagcgcat ctaacacatc
    16321 gatccagtcc atcccggcaa gctgatagaa gtgcaccaga tgatcgtggc accagagcgt
    16381 tgccagcata atgttgcgga tgatattggc gttgtccggc actttaatac cgatagcatc
    16441 ttcgatggcg taaaccgaag ccagggcgtg tacgccagta cagacgccgc agatacgttc
    16501 aacgaacgcc cacgcatcgc gcgggtcgcg cccttgtagg atgatctcca gcccgcgaaa
    16561 catggtgccg caggagacgg cattggtgat cacattctga tcgttaatat tcacttcgca
    16621 gcgcatgtgg ccttcgatgc gcgtaatcgg gtcgaccacc aggcggcgtc cggcattatt
    16681 gatggtgtat ccctgagttt cgtactgagt gctcatgcct gtttatcctc attgcctggc
    16741 tgatgttcgg tttctgtagg ttgctggtta tgacgtctgc gctggtcaac ggcgctggcg
    16801 actgcgtgca caccaacagc cgctgccacc acaccaagcg cggttaaacc gacggtatcg
    16861 gcggtggaat gagtacccat ttgcggaata tcgaccacgc ggctgtagaa cgaaccgcaa
    16921 tcccagaaac cattttccgc acagcccagg cagccgtgac cagactggat tgggaaagaa
    16981 acgccatcat tccagcgtgt ggaggaacag gcgttatagg tggtaggccc tttgcagccc
    17041 attttgtaca ggcagtaacc tttgcgggca gcgtcatcat cccaactctg gacgaactct
    17101 ccggcgtcga agtgggcgcg gcgatagcat ttatcgtgga ttcgctgacc atagaacatc
    17161 agcggacggc ccattctgtc gacatctggc aagcgatcaa aggtcaccat gtaagtaatg
    17221 atggcgctca tcacatccgg gatcggcggg cagccaggta ctttgataat gggtttgtcg
    17281 gtgatgactt tgtcgatagg cgttgcctgc gtcggattgg gccacgcggc ctgcacgcag
    17341 ccccaggacg cgcaggttcc ccaggcgata atcgcgctgg ctccggcagc ggcacgtttg
    17401 agtttctcaa taaacggtcg accgctgctg atacagaaca tcccctgctc gcccagcggc
    17461 ggattacctt ctactgcgag gatatatttg ccattgtatt gcgtgatgat gtcttcgaag
    17521 acttcttccg cctgggttcc ggcggcagcc atcaaagtat cgtcataatc gagggaaatc
    17581 agggaaagga tgacgtcctt cgccagtggg tgagcggagc ggataaaaga ttcggtacag
    17641 caggtgcatt ccagaccgtg gatccatacc accggaatgc ggggtttgtt ctccagcgcc
    17701 caggcaatct ttggtgccat tcccgcgcct aatcccagcg acgtggcagc cagactacaa
    17761 tatttgagaa agctgcgccg ggtaacgccc tgacgccgca tggcctggta aaatgtttcc
    17821 tcgttattca tatcgcacgt ctcttctcct tgcgacaccg gcaggacacc gggcgcttat
    17881 ggtcggcaag cgacgtccct gtagcaaggc aaaccatcgc ttcacgtgag atgctgaaaa
    17941 cgaaagctca tccttctgca ctggcgcacg tcgccagaaa gtattgttaa taaagcgtag
    18001 tgaaa
//
LOCUS       DAZOZD010000069        17866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOZD010000069 DAZOZD010000000
VERSION     DAZOZD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.069"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            139..817
                     /locus_tag="ACHUPB_001905"
                     /pseudo
     CDS             139..817
                     /locus_tag="ACHUPB_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            861..5711
                     /locus_tag="ACHUPB_001906"
     CDS             861..5711
                     /locus_tag="ACHUPB_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR0758730.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6079..7734
                     /gene="lldP"
                     /locus_tag="ACHUPB_001907"
     CDS             6079..7734
                     /gene="lldP"
                     /locus_tag="ACHUPB_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0758731.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7734..8510
                     /gene="lldR"
                     /locus_tag="ACHUPB_001908"
     CDS             7734..8510
                     /gene="lldR"
                     /locus_tag="ACHUPB_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0758732.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8507..9697
                     /gene="lldD"
                     /locus_tag="ACHUPB_001909"
     CDS             8507..9697
                     /gene="lldD"
                     /locus_tag="ACHUPB_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0758733.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9893..10366
                     /gene="trmL"
                     /locus_tag="ACHUPB_001910"
     CDS             9893..10366
                     /gene="trmL"
                     /locus_tag="ACHUPB_001910"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0758734.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="ACHUPB_001911"
     CDS             complement(10419..11240)
                     /gene="cysE"
                     /locus_tag="ACHUPB_001911"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0758735.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="ACHUPB_001912"
     CDS             complement(11320..12339)
                     /gene="gpsA"
                     /locus_tag="ACHUPB_001912"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0758736.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12339..12806)
                     /gene="secB"
                     /locus_tag="ACHUPB_001913"
     CDS             complement(12339..12806)
                     /gene="secB"
                     /locus_tag="ACHUPB_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0758737.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="ACHUPB_001914"
     CDS             complement(12869..13120)
                     /gene="grxC"
                     /locus_tag="ACHUPB_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0758738.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13262..13693)
                     /locus_tag="ACHUPB_001915"
     CDS             complement(13262..13693)
                     /locus_tag="ACHUPB_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0758739.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13938..15482
                     /gene="gpmM"
                     /locus_tag="ACHUPB_001916"
                     /gene_synonym="pgmI"
     CDS             13938..15482
                     /gene="gpmM"
                     /locus_tag="ACHUPB_001916"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0758740.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15516..16775
                     /gene="envC"
                     /locus_tag="ACHUPB_001917"
     CDS             15516..16775
                     /gene="envC"
                     /locus_tag="ACHUPB_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0758741.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16779..17738
                     /locus_tag="ACHUPB_001918"
     CDS             16779..17738
                     /locus_tag="ACHUPB_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0758742.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17725..17866)
                     /locus_tag="ACHUPB_001919"
                     /pseudo
     CDS             complement(17725..17866)
                     /locus_tag="ACHUPB_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4479 a         4625 c         4673 g         4089 t
ORIGIN      
        1 tttttatgat gaatgcttaa aacaatattt aatcgacaca catcactgtc attaatgtaa
       61 agcaacatag atcaccaata atacaatgga caactctacc ggcattgatc gctggtcgga
      121 ggaaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt cactgtttca
      181 gttctctgcg cattaacggg atgtgactac attgaagaag gcaagccaga aagtagttta
      241 ttgaaacaac aagaagaaca ttaaaattga tctacttgaa aaacaacaag cacagctaaa
      301 gagtcaactg gaaactattc aaaaacaaca aactggcata ataagcagta caaagacttt
      361 aactcacgta attaaatccg ttaaagatca acaaaacact tttattttta ccgagtttaa
      421 cccagcaaaa acgaagtatt tcatcctgaa caatggttcg gtggctttag cgggtcgagt
      481 gttatccatt gacgccacag agaatggcag tgttattcat atttcactgg tcaacttatt
      541 aagtacacct atctcaaata ttggttttaa tgcgacatgg ggtggtgaaa aacctgtcga
      601 tgccaaagag tttgcccggt ggcaacaatt acttttcaac acatcaatga aatccacatt
      661 gaaattatta ccaggtcaat ggcaagacat taatttgacc ctgaagggtg tatcgcccaa
      721 taacctggga tatctgaagt tagccatcaa catggaaaat attcagttcg acaatcttcc
      781 ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg cagttagtac gcttataaca
      841 aggaatagcg agcatcatta atgaacaaaa tatttaaagt tatctggaac cctgcgacag
      901 ggaattatac tgttaccagc gaaacggcaa aaagccgtgg caagaaatct gggcgcagta
      961 agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc gtcgtttggg gcattggcga
     1021 atgccgggaa tgacaacggc cagggtgttg attacggtag tggatcagct ggcgacagct
     1081 gggttgctat aggcaaaggg gcgaaagcaa atacttttat gaacaccagt ggttccagta
     1141 ctgctgtggg ttatgacgct atagctgaag gccaatatag ctctgccatc gggtcaaaaa
     1201 cccatgcgat tggtggtgca tcaatggcct ttggggttag tgcaatatca gaaggcgata
     1261 gaagtatagc actgggtgcc tcttcgtatt cattgggcca atactcaatg gccctcggcc
     1321 gttattcaaa agcattgggt aaattgtcta ttgctatggg ggactcttcc aaagcggaag
     1381 gagcaaacgc cattgccctg ggaaatgcca ctaaagctac tgagattatg agtattgctc
     1441 ttggcgacac cgccaatgcg tcaaaagcgt attcaatggc gctgggagca agtagcgtcg
     1501 catctgaaga aaacgcaatt gccctggggc gtagcagtgt agctagcggt actgacagcc
     1561 tcgcatttgg cagacaatca cttgccagcg cagcgaacgc tattgcgata ggtgctgaga
     1621 ccgaagccgc tgaaaatgca actgctattg gcaataatgc gaaggcaaaa gggactaata
     1681 gcatggcaat ggggttcgga agccttgccg ataaagtcaa tactatcgca ttaggaaatg
     1741 gcagccaggc tctggcagat aatgcaatcg ccataggcca gggcaacaaa gctgatggcg
     1801 tggatgccat cgctctgggt aatggtagcc agtcgagagg cttaaacacc attgccttag
     1861 gcacagccag taatgcaact ggtgataaga gtcttgcgct tggtagtaat agcagtgcca
     1921 acggtattaa ctctgtcgcg ctgggcgcag attccattgc ggatttagac aataccgtct
     1981 ctgtcggcaa tagttcatta aaacgcaaga tcgttaatgt gaaaaatggc gcgatcaagt
     2041 ctgacagtta cgatgccatt aatggttcac agctttatgc cattagcgac tcggtagcaa
     2101 aaaggcttgg aggaggggct gcagtagatg ttgatgacgg tactgttaca gcaccaacct
     2161 acaatttaaa aaatggtagc aaaaataacg taggggctgc gctcgctgta cttgatgaaa
     2221 acaccctgca atgggaccaa accaaaggca aatacagcgc tgctcatggt actagtagcc
     2281 caactgccag cgtaatcacc gatgttgcgg atggcacgat ttcagcctcc agtaaggatg
     2341 cggttaacgg ttcccaactg aaagctacca atgacgatgt cgaagccaac accgccaata
     2401 tcgctactaa taccagcaac attgccacga atacagcaag tattgccacc aataccacca
     2461 atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg ctctggaacg
     2521 aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa atcaccaacg
     2581 ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct cagctgaaaa
     2641 ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact tccaatattg
     2701 ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt ggtgaggatg
     2761 cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc gagaccacca
     2821 gcaaaatcac caacgttaaa gatggcgacc tgacgactgg cagcaccgat gccgttaacg
     2881 gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat atcgccacta
     2941 acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga
     3001 aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc gccagcaaaa
     3061 tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt aacggtagcc
     3121 agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct tctgtgaata
     3181 ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat tataacgtcg
     3241 gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat gctctgcttt
     3301 gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac gcaagcgtga
     3361 tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta aacggctcac
     3421 aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc gaagtcaatg
     3481 cagacggcac catcactgcg ccgacgtaca ccattgctaa tgctgattac gataatgtcg
     3541 gtgatgccct gaatgctatc gataccactc ttgacgacgc tctgctctgg gatgcggacg
     3601 ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga taaaactgcc agtgtaatca
     3661 ctaacgtcgc taacggtgca atctctgctg ccagcagcga cgcgattaac ggctcacaac
     3721 tctataccac caataagtac atcgctgatg cgctgggtgg tgacgcagaa gtcaacgctg
     3781 acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac aacgtcggtg
     3841 acgccctgga tgcgcttgat gataacgccc tgctgtggga tgagactgcc aatggcggtg
     3901 ctggagccta caatgccagc catgacggta aagccagcat catcactaat gtcgctaatg
     3961 gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat gcgacgaata
     4021 tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc gacgcaacct
     4081 atatcgaaga aaatggcgct ggtatcaact acgttcgtac taacgacaac gatttagcct
     4141 ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt aggttataac gctgtcgcgt
     4201 ctggtgccag cagcgtagcc attggtcaga acagcagcag caccgttgat accggtattg
     4261 cgctgggtag cagctccgtt tccagccgtg tgattgccaa aggttctcgt gacactagcg
     4321 taacggaaaa tggcgtggtg attggttatg acaccactga cggcgaactg ctaggtgcat
     4381 tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa cgtagctgat ggttcagaag
     4441 cccatgacgc cgttacggtt cgccagttgc agaacgctat tggagcggtc gccactacgc
     4501 caaccaagta ctttcacgcc aactcaacgg cagaagactc actggccgtt ggtgaagact
     4561 cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc aggtattggt atcggtttga
     4621 acactctggt tctagctgat gcgatcaacg gcattgctat cggcagtaac gcacgcgcaa
     4681 accatgcaaa cagtatcgca atgggtaacg gttctcagac cacccgtggt gcacagactg
     4741 gctacaccgc ctacaacatg gacgcaccgc agaactctgt aggtgagttt tctgtcggta
     4801 gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg ttcagcggat accgatgcgg
     4861 ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc gcaaaatacc cagagcatta
     4921 ctaacctgaa caatcaggtc actaatctgg atactcgcgt tactaatatc gaaaacggta
     4981 ttggcgacat tgtcaccacc ggtagcacca agtacttcaa gaccaacacc gatggcgtag
     5041 atgccaacgc ccagggtaaa gatagcgttg ctattggttc tggttccatt gctgccgctg
     5101 acaacagcgt cgcactgggt accggttccg ttgcagagga agaaaataca atctctgtag
     5161 gttcttctac taaccaacgc cgtatcacca acgtagctgc cggtgttaat gccaccgatg
     5221 cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg cgtgcgctac gacaccaaag
     5281 ctgatggttc tatcgactat agcaatatca ccctcggtgg tggcaacggc agtacgactc
     5341 gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac gcgcagttga
     5401 agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg gatggttgag atggataaca
     5461 aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg gcaatgaccg
     5521 gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc ggtacttaca
     5581 acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt cgttgggtct
     5641 acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc ggtgccggta
     5701 ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa ttgcctgatg
     5761 cgttacgcgc atcaggcaag ataaggcgcc ccgggtcaac aatctaaggg caattctctg
     5821 atgaggattg cccttttctt taccagacat ctccccccac aagaattggc cctaccaatt
     5881 cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc aatattgcca
     5941 atacataaca tttagttaac cattcattgt cattatccct acacaacaca attggcagtg
     6001 ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac gatgtgcgtg
     6061 gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc cgggaatatc
     6121 tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc gctgattaag
     6181 ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct tgccgtggct
     6241 ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta tggcttcttc
     6301 tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta taagatctcg
     6361 gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac ccctgaccag
     6421 cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg agccgcaggc
     6481 tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt taaaccgctg
     6541 tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg cgcgatgggc
     6601 attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat tggccagatg
     6661 gtggggcggc agctaccgtt tatgaccatt atcgtgctgt tctggatcat ggcgattatg
     6721 gacggctggc gcggtatcaa ggagacgtgg cctgcggtcg tggttgcggg cggctcgttt
     6781 gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga cattatctct
     6841 tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcagcc agtgcgcgta
     6901 ttccgttttg gtgatttggg ggcgtcaaag attgatatga cgctggccca caccggttac
     6961 actgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgacagc taccgtaaca
     7021 ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg
     7081 gtgatcaaca ttccggtgcc gtacctcgat aaactggttg cccgtatgcc accagtggtc
     7141 agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc caccggcacc
     7201 gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc gtctgacgct
     7261 atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc catcggcatg
     7321 gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact ggcactggca
     7381 ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg gctgggggta
     7441 ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca agccaccgca
     7501 gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac cggtggcgtc
     7561 accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt aggcctggtg
     7621 ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt cacctgtatg
     7681 gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc ttaatgattg
     7741 ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa
     7801 aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg caactcggcg
     7861 tatcacgtaa ttcactgcgc gaggcgctgg caaaactggt gagcgaaggc gtgctgctca
     7921 gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg gagcaaaaca
     7981 tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc gatattctgg
     8041 aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc gccacacctg
     8101 gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac ccggatatcg
     8161 cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat aacatcgtgc
     8221 tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc
     8281 agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat caggctgtca
     8341 ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta
     8401 gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac gcacggatta
     8461 cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga ttatttccgc
     8521 agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt tccactatat
     8581 ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt tgtcagaagt
     8641 ggcactgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa cgacgctgtt
     8701 taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc
     8761 gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg catggcattc cgtttactct
     8821 ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg
     8881 gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa
     8941 agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg gcgcacgcta
     9001 ccgtgatgcg cattcaggta tgagcggccc aaatgcggca atgcgccgct acttgcaggc
     9061 ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac atgatttagg
     9121 taatatctca gcttatctcg gcaaaccgac cggactggaa gattacatcg gctggctggg
     9181 gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg atttctggga
     9241 tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg cagtacgttt
     9301 tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg gtgtactctc
     9361 ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc
     9421 ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac
     9481 cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc
     9541 taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa
     9601 atcgatcagt gaaattacgc aagattcgct ggtgcagggg ctgggtaaag agttgcctac
     9661 ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa
     9721 ccctctccct cagggagagg ggaccgttcg gcgctgtatg tactccctca ctctgaaacg
     9781 acaccgcact ctttttttct ccctcgcccc tccggggagg gccggggtga ggggaaaagg
     9841 ccgcacggtt caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa
     9901 catcgtactt tacgaaccag aaattccgcc aaatactggc aacatcatcc gtctttgcgc
     9961 taataccggc tttcgtctgc atatcatcga accgatggga tttgcctggg acgataagcg
    10021 cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgacta
    10081 tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac
    10141 gcctgctcac agcgccgtaa gctatcagga tggcgattat ctgatgttcg gcccggaaac
    10201 acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc
    10261 gatggtgccg gacagccgca gcatgaatct gtccaatgcg gtgtcggtag tggtgtatga
    10321 agcctggcgg cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg
    10381 atagatgatt acatcgcatc cggcacgatc acaggacatt agatcccatc cccatactca
    10441 aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct
    10501 ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc
    10561 agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt
    10621 ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat
    10681 ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc
    10741 gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct
    10801 gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat
    10861 gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa
    10921 cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc
    10981 gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttcc
    11041 accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg
    11101 tagctcagtg cactaccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc
    11161 agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt
    11221 tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg
    11281 cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtggctgc tgcgctcgtc
    11341 cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa
    11401 tacttgataa atttcctcgg ttattggcat ttctacgccg aagcgatgcg ccagttcgcg
    11461 gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg
    11521 tacatccatg ccctgaccga gcatcatgcc aaaacggcgg ttacgcgact ggttgtcggt
    11581 acaggtaagc accagatcgc ccagccccgc catgcccata aaggtggcag gatcggcacc
    11641 cagcgccgcg ccaagacgcg acatttcagc cagcccacgg gtgatcagtg ccgtacgcgc
    11701 attcgcacca aaaccgatac cgtcggacat ccccgcgcca atggcaatga cgtttttcac
    11761 cgcaccgcca agctgcacac cgatgaaatc cgggttgctg taaacgcgga aacttttgcc
    11821 acagtgcagc aattgttgga gatcatcggc aaaggtctgg tcggtagagg ccaacgaaat
    11881 agccgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag
    11941 cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc
    12001 ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg
    12061 gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac ggctggctgc
    12121 cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag
    12181 aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca
    12241 gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc cgtacgagcc
    12301 ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca
    12361 gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaatcggc
    12421 gcaaggttca gttgcgggaa tgtaccgcgg gataccacgc tggtgatgca ctcacgagca
    12481 tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg
    12541 atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg
    12601 cccagggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg
    12661 gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc
    12721 ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc
    12781 atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac
    12841 tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg
    12901 catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg
    12961 gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga
    13021 tcggcaactc ctgaaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat
    13081 acgggcaggt ttctttggta tagatttcaa cattggccat aatttagctc ctgttttttt
    13141 accctgaaat ttcatgttgc agggcggctg caaaatggag tgtccccaga agcttataac
    13201 aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa
    13261 attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg ccctctttca
    13321 gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca
    13381 tgccagaacc gtctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat
    13441 tgttggcttt gatttcgctc ggcaacagat tgatagaacc tgcgatatgg cctttacgga
    13501 agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag
    13561 cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa
    13621 ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca
    13681 taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga
    13741 gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg
    13801 gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg
    13861 atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg
    13921 agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg
    13981 gatatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa
    14041 tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg
    14101 gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc
    14161 gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg
    14221 ctaatccagt gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa gcagtacaca
    14281 ttatgggtct gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg
    14341 tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc
    14401 gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg
    14461 cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata
    14521 accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagtttc
    14581 aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt
    14641 tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg
    14701 atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg
    14761 tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc
    14821 tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta
    14881 acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg
    14941 aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg
    15001 aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga
    15061 gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca
    15121 ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcgg
    15181 ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg
    15241 tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg
    15301 caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt tacgttggtg
    15361 ataagaacgt gaaagcggtt gcaggcggca aactttctga catcgcgccg accatgttgt
    15421 cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat
    15481 aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca
    15541 ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg
    15601 ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgcca
    15661 caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga
    15721 aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc
    15781 tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc
    15841 aaaaagccgc ccaggagcgc agcctcgccg cacaactgga tgccgcattc cgtcagggcg
    15901 agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga cagcgtttac
    15961 aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa
    16021 cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga gaaacaaagc gagcaacaaa
    16081 cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggctctg aacgagcgta
    16141 aaaagacgct ggcggggctg gaatcttcca tccagcaagg tcagcagcag ctaagtgaac
    16201 tgcgtgccaa cgaatcccgt ctgcgtaaca gtattgcccg tgcggaagcc gcggcgaaag
    16261 cgcgtgctga acgagaagcg cgtgaggctc aggcagttcg cgacaggcag aaagaagcga
    16321 cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg
    16381 gtggcctggg tgcgccgcgt ggtcaggcat tctggccagt tcgtggccct acgctgcatc
    16441 gctatggcga gcagctacag ggtgaattac gctggaaagg aatggttatc ggtgcctctg
    16501 aaggtactga agttaaagca attgccgacg gtcgggtgat tctggctgac tggctgcaag
    16561 gctacggtct ggtggtggtg gttgagcacg gtaaaggcga tatgagtctt tacggctata
    16621 atcaaagcgc gctggtgagc gttggttcgc aggttcgtgc gggccagcca attgcactgg
    16681 tgggcagcag tggcggtcag ggcagacctt cactctattt cgaaattcgc cgccagggtc
    16741 aggccgtcaa tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg
    16801 ttcttgcatt tgccgctctg ttggcgctct cctcccctgt aattgctggc aaacttgcca
    16861 tcgtcattga tgattttggg tatcgcccga acaacgaaaa ccaggtgctg gcgatgcctt
    16921 ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag
    16981 cgcataacag cggtcacgaa gtgttgattc atctcccgat ggcaccgttg agtaaacagc
    17041 cgctggagaa aaatacgctg cgcccggaga tgagcagcga cgaaattgag cgcattattc
    17101 gtagtgcggt caataacgtg ccctatgccg tggggatcaa taaccacatg ggcagcaaga
    17161 tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tataatcttt
    17221 acttcctcga cagcgtaacc attggtaata cccaggcgat gcgcgccgcg caaggcactg
    17281 gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc acagaacgaa gcggacatcc
    17341 gtgtgcaatt caatcgcgca attgacctag cgcgtcgcaa cggatcaacc attgccattg
    17401 ggcatcctca cccttcaacg gtacgcgtgt tgcaacagat ggtttataac ctgccgccag
    17461 acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acgtctacac
    17521 cgccgaaaaa cactgtgccc gacacaccgc gtaatccgtt ccgtggcgtg aagctgtgca
    17581 aaccgaagaa accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa
    17641 gcatcagcca gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac
    17701 agcctgaagc ggcgaagctt aacgctagcg taaattaaag gcgctacgcg taatgcgatg
    17761 gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa gactacagta acgcctgata
    17821 acccaccttt acgctgcgca cattggtaat caggcgctta tacata
//
LOCUS       DAZOZD010000070        17709 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOZD010000070 DAZOZD010000000
VERSION     DAZOZD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.070"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            42..3809
                     /locus_tag="ACHUPB_001920"
     CDS             42..3809
                     /locus_tag="ACHUPB_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR0758743.1"
                     /translation="MPSVSRYRTWLAVPADEIEDLKKAHPPMNGHTPVIWDKEHKLWF
                     ARPGADLSRLDRWLPRPQDVSMNGSDPVTEFAQVLENAGLVLKELPVMDGKIHRVPTA
                     DDKKGQKSGAYRGFLDGRPAGWYRDYRSADNSPITWTFSGGEQTDPRARLHLKAHSMQ
                     RREDAERELKAQYNRQAAYARRYINKWPQATAHEYLTRKGIQAAPGVRVNNKNELVIP
                     FSNRNGAIRSYQRIPVTGGKDARILIDSEKTGNWFALGTPRNGQPVLFAEGYATAASL
                     HEATGLPVLMTVDGGNMIAVAENARQKWTQSPFIFCADNDHAIRVNKGIVSATKAAEL
                     TGGSVIFPAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEMR
                     EALVIGNLVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPAASSSNI
                     ADETQSFTSHATENNGKDERHADNVQAQTESSVVQDSPAESPASPESTTASAPDEPAH
                     PAEPPEKVVSVATDKDWREFEAELSQPEKNESQQESGTSPEIPAPAAAPASPEDSPSS
                     ETMNESAEPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVNHVTEDIPLQPEPF
                     LPESDGPEEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGHVQADESELQATTATIE
                     PAVRDEPTPQQPVQSTSPSDNTSSFLDKARGFFTRKKTDSQAHENSDPTPSAETTTTP
                     PIPDSIVYAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMV
                     NGASNDDRSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAE
                     LEKLRKEMAVSKDAVVTHQPTPELNRNIPEQPAVPDPVQEKEATQSPASPAAPEASTV
                     STAPASSPAEPGKTADAAPGEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELK
                     NRSGSKLYWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNG
                     ISVSHDNPDKGQRIQAIPVETFMKVAAQISQGWPEEIKALRMPENVGSHLFIGEDRHP
                     VSAPQNANQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLY
                     PALATPSADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPL
                     FAAVYTPEKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            3827..4174
                     /locus_tag="ACHUPB_001921"
     CDS             3827..4174
                     /locus_tag="ACHUPB_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0758744.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            4171..4863
                     /locus_tag="ACHUPB_001922"
     CDS             4171..4863
                     /locus_tag="ACHUPB_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFR0758745.1"
                     /translation="MTENTELSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFVGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            4874..5857
                     /gene="traN"
                     /locus_tag="ACHUPB_001923"
     CDS             4874..5857
                     /gene="traN"
                     /locus_tag="ACHUPB_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HFR0758746.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            5860..7149
                     /gene="traO"
                     /locus_tag="ACHUPB_001924"
     CDS             5860..7149
                     /gene="traO"
                     /locus_tag="ACHUPB_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFR0758747.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGGGYIAFSKLSGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDSQ
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNQSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            7149..7853
                     /gene="traP"
                     /locus_tag="ACHUPB_001925"
     CDS             7149..7853
                     /gene="traP"
                     /locus_tag="ACHUPB_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HFR0758748.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMVWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            7853..8380
                     /gene="traQ"
                     /locus_tag="ACHUPB_001926"
     CDS             7853..8380
                     /gene="traQ"
                     /locus_tag="ACHUPB_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFR0758749.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            8431..8835
                     /gene="traR"
                     /locus_tag="ACHUPB_001927"
     CDS             8431..8835
                     /gene="traR"
                     /locus_tag="ACHUPB_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HFR0758750.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKYLLLIPMMLLPKA
                     VLADGDLADMVRNVGEGAKTAQSSSITIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGAVLVAGPEMMSRSQKQLGISSISIG"
     gene            8899..9087
                     /gene="traS"
                     /locus_tag="ACHUPB_001928"
     CDS             8899..9087
                     /gene="traS"
                     /locus_tag="ACHUPB_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HFR0758751.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            9071..9871
                     /gene="traT"
                     /locus_tag="ACHUPB_001929"
     CDS             9071..9871
                     /gene="traT"
                     /locus_tag="ACHUPB_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HFR0758752.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            9961..13005
                     /gene="traU"
                     /locus_tag="ACHUPB_001930"
     CDS             9961..13005
                     /gene="traU"
                     /locus_tag="ACHUPB_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HFR0758753.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGLGSLESLITSMSDSLNTAY
                     KNSGHKISCVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEDLVDSALQDSGLYEKHDAAWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEDLASELIRKQGYNL"
     gene            13005..13619
                     /gene="traV"
                     /locus_tag="ACHUPB_001931"
     CDS             13005..13619
                     /gene="traV"
                     /locus_tag="ACHUPB_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HFR0758754.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRLFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            13586..14788
                     /gene="traW"
                     /locus_tag="ACHUPB_001932"
     CDS             13586..14788
                     /gene="traW"
                     /locus_tag="ACHUPB_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HFR0758755.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            14817..15401
                     /gene="traX"
                     /locus_tag="ACHUPB_001933"
     CDS             14817..15401
                     /gene="traX"
                     /locus_tag="ACHUPB_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HFR0758756.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            15498..17666
                     /locus_tag="ACHUPB_001934"
     CDS             15498..17666
                     /locus_tag="ACHUPB_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFR0758757.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVREDRLKNVTKDDNKDGIKG"
BASE COUNT         4778 a         4624 c         4421 g         3886 t
ORIGIN      
        1 cggcggtggt ttttttccgt cttttttcag gagaagcact catgccgtct gtatcccgct
       61 accgcacctg gcttgccgtg cctgcagatg aaatagaaga tctcaaaaaa gcacatcccc
      121 cgatgaacgg gcacacgccc gtcatctggg acaaggagca caaactctgg ttcgcccgcc
      181 cgggggcgga cctgtccagg ctggaccgct ggctgccccg gccacaggac gtctccatga
      241 acggcagtga tccggtcacc gagtttgccc aggttctgga gaatgccgga ctggttctga
      301 aagaactgcc ggtcatggac gggaaaatcc accgtgtccc gaccgccgat gacaaaaaag
      361 gacaaaaaag tggcgcatat cgcggattcc tggacggcag acctgccggc tggtacaggg
      421 attatcgcag cgcggataat tcgccgatca cctggacctt ctccggcgga gaacagacag
      481 atccccgcgc aagactccac ctgaaagcgc attcaatgca acgcagggag gacgcggagc
      541 gggaactgaa agcacagtac aaccgacagg ccgcgtatgc ccgccgctat atcaacaaat
      601 ggccgcaggc cacagcacat gaatacctga cccggaaagg cattcaggct gcaccggggg
      661 tgcgggtaaa caacaaaaat gaactggtta ttcccttcag taaccgtaac ggcgcgatac
      721 gctcttacca gcgtattccg gtcaccgggg ggaaagatgc ccgcatcctg atagattcgg
      781 agaaaaccgg taactggttt gcgctgggca caccgcgcaa cggtcagccg gtactgtttg
      841 ccgagggcta tgccactgcg gcctccctgc acgaagccac gggccttccg gtactgatga
      901 cggtggatgg aggcaacatg attgccgttg ccgaaaatgc ccggcaaaag tggacgcaaa
      961 gcccctttat cttctgtgcc gacaacgatc atgcaatccg ggttaacaaa ggtatcgtca
     1021 gtgcaacaaa agccgcagaa ctgacaggag gatcggtcat ctttcctgcg ttcaccgatg
     1081 ccgaaaaagc gcaggggctg accgatttca atgacctgga cgcaagccgg ggacgggccg
     1141 cttttcagca tgtcatcaac gcccagttag aacacatcgg ggtttccacc cccaacagta
     1201 acaccccgga aatgcgcgag gcactggtga taggcaacct cgtttttaca ccggtccaca
     1261 ctgaggaaaa aaccatgact ccgacagaat atcctgaaac atcacccgat acagggcaca
     1321 gccatgacca gggaccttct ctgccgtccg ccacgcagca ggaacaacca gctgcttcca
     1381 gcagtaacat tgctgatgaa acacagtcat ttaccagtca cgccactgaa aataacggca
     1441 aagatgagcg gcacgctgat aacgttcagg cgcaaacgga aagctctgta gttcaggact
     1501 cccctgcaga atcaccggcg tccccggaaa gtacaacggc gtctgcccct gatgaacccg
     1561 ctcatccggc ggaacctccg gaaaaagttg tctcggtggc cacagataaa gactggcgtg
     1621 aatttgaagc cgaactgtca caaccggaaa aaaacgagtc acaacaggaa tccggcactt
     1681 cacctgaaat accggcccct gctgccgccc ccgcatcccc tgaagacagt ccctcatctg
     1741 aaaccatgaa tgaatcagcc gagccatcac cttcccctga tgcagtcaaa gaggaaatga
     1801 ccgtgatgac ggagcctgat gacaaacaaa gcaccggaat atccccggat actccccggg
     1861 aagaggcggt caaccatgta actgaagaca ttccccttca gccagagccg ttccttcctg
     1921 agtcagatgg ccccgaagaa tacgaagact acagcgcata tcaggagctg atgaacaatg
     1981 aaaatcctga gcatctgcag gaaaacagtg atatgccgtc gcaaccggac acaggccacg
     2041 ttcaggcaga tgagtcagaa cttcaggcca caacagccac tatagaaccg gctgtccggg
     2101 atgaacccac tccgcagcaa cctgttcaga gcacatcacc ttctgataat acttcatcgt
     2161 ttctcgacaa agcgcgtggc ttttttacac gtaaaaaaac ggattcacag gcgcatgaga
     2221 acagtgatcc cacgccctca gcagaaacca cgacgactcc accgatacct gacagtattg
     2281 tctacgcccc ggaacgttct gatgcaccga tatcgctcaa catggacgaa attcttaaag
     2341 agcttgactg ggaaaaacga gcagacagaa ccgtcctgta caaactggat ggcaaacctg
     2401 ccttcattga ccgggtaaac cggctggaaa tggtcaacgg tgccagcaat gacgatcgca
     2461 gcgttctggc cgcacttgct gtggccacca aattttatgg gggcgtcata gagctgacgg
     2521 gcagtgatgc ctttaagcaa aaagcaatgc agttgattat cgagcacaac atcgatgtcc
     2581 ggatgaaatt gccagatcaa cgggctgaac tagaaaaatt acgcaaggaa atggctgtca
     2641 gtaaggatgc tgttgtcacc catcagccaa caccggaact taaccggaat atccctgaac
     2701 aaccggctgt ccctgacccc gttcaggaaa aagaagccac acaatccccg gcgtcccctg
     2761 ctgcccctga agcatcaacc gtcagcaccg ctcctgcatc atcaccggct gaaccaggca
     2821 aaacggcaga tgcagcacca ggtgaagaac caccaggaaa acttcgcccg ggagagagcg
     2881 tgactgccgt actgcacaat tttggtcgtg ctgaatatgc acccggcaaa ggagaaagtt
     2941 tctttgttga gcttaaaaac cgcagtggca gcaaactcta ctggggggaa caactggaaa
     3001 gtctggtcaa aaaccatcag aaaggtgacg tggtgacact gacacttcag aaccgcgaac
     3061 aattcatcct cccgggagag caaaaagcac gtttccggaa taagtggtcc atggagtcag
     3121 ttaccaacgg gatctcggta tcacatgata atccggataa aggtcagcgt attcaggcga
     3181 ttccggtcga aacttttatg aaagtggctg cgcaaatcag ccaggggtgg cccgaagaga
     3241 taaaagcgtt acgtatgcca gaaaacgtcg gctcacatct gtttatcggt gaagaccgac
     3301 atccggtctc cgcgccacag aacgcaaatc aggtaacaga aataaccagc gcagcccctg
     3361 acaagctgac acctgttctg ggatcagtgg ataaggatac gcgtgaactc aacctgctac
     3421 ttgtccagag tgccgatgaa catctgcagg gtgtggtccg gctgaacggc acattgtatc
     3481 cggcactggc caccccatcg gccgacaaca gtcagctggt tatcaatgcg ctgaccgaca
     3541 aaggtttacg ctttgcaggt tatggtgaag cggtaaacca cgatgctgac agcacgaatc
     3601 gccccgcacc ggagcttatg cagtttcatc tgaaaacccg ggaagaaccg ctttttgctg
     3661 ctgtctacac gccggaaaaa caacctgatg cgttgtaccg gaacctgggc ttcgagcagt
     3721 catggcagca gtggtccaac agccaaaagc cggaggacag gcaggaaaaa actctgcatc
     3781 aggatctctc ccacagcccc ggccgatgag ccggtaagga agccatatga acatgcataa
     3841 gtctctggtt gccgtgctcg gccttatcct gatttcatcc acggcaagcg ctgcaacctg
     3901 tcgtgcgggt agcgcggcac aggccggtag taatacagga tatgagcgag cacgtgcagc
     3961 tgcggatgca tggtcacaac gtgaaaacga tgtttcctca tccctgcagt cctgtctgtc
     4021 acgaatcaaa aaaatcagca taaacctccc ccagttcccc agccttgacg acattctgtc
     4081 gcagctggaa aaccaggtct gcgacgcggt cgtggacaag gtgaatgaaa agctgccggg
     4141 gaatattgat ccctggaagg actacaacct atgaccgaaa ataccgagtt atcctcgcca
     4201 tcacatccgg cacctgaccc ggaaatactg aagagcacaa tcagggctat gaagcagtca
     4261 gaacagcggg caaataatgt ccctgcgctg ataaaagcgc ttttatgtac gggcacatgt
     4321 cttcttatca gtattactgg taacgccatt cagtactggc actcaacgaa tgtggagcgc
     4381 gaatatttcg ctacagacaa tggtcgtctg gtcaggctgg caccaacaag tcagccagca
     4441 tggagtcaga atgatgcaat ggcctttggc agccaggcac tggcgaccgc attcaacctg
     4501 gattttgtgc actatcgttc acagatctcc tccctgtctc cgcgtttttc tgacgaaggt
     4561 tttgtcggat atgtgaatgc attacaggca tctaacattc tggagacgat caagaaagag
     4621 aagatgaatc tgaccgccac gacaggtgct ggcgttctgg tacgacaggg acaaatgtcc
     4681 gacggcgtat ggttctggac attccagtat cctgtccgta tgcgtctggt cggccagaca
     4741 accagcaagc cagaacagtc ttttgttttc gaaatcacca tccagcgtgt tgaccccaga
     4801 ctgaaaccat ccggtatgga aatccggcag atgatctccc gtaacgccgg accgaactcc
     4861 tgagataagt aatatgcaat cacgttattt actgagcaca ctgctcctgg tgtgcagcgc
     4921 cgccacgtct gcagataacg caggatggca gaacgcacgc acaccacaaa cgacaaattc
     4981 tgccagcgat cacgttcaga acgcctccca aaacaccgga ggtgttccgg ctacactggt
     5041 aaaaggtgag ctgcctgccc ccgggcaggc cagcccgctg gtacaggatg cggcaaggct
     5101 tgattctgag ctctccgccg atgagattcg ctcactacgc agtctcatgg cggataacga
     5161 gcgggccatc aatgcgccca tcaccagtgt tgtaccccgt atcagctctc tgacagttaa
     5221 tctgtccccc ggtgccagcc ttcctcttgt tcgcacagcc atgaacaacc tgagcgtggt
     5281 gacatttacc gatatcaacg gttcaccatg gccacaatcc gatcctcctt ataacgcggc
     5341 accaaaactc tttgatgtgc agtacaacga gaacatggtg acaatcacac cattgcggcc
     5401 atgggcatcc ggaaacattt ctgtctacct gaaaggtctg tcggtaccgg tcatcctgaa
     5461 cgtaaccagt ggcgaaacag atacgccgtc atccagtcag gaaatggaca gtcgtctgga
     5521 tctgcgaata ccacggcagg gaccaaccag cccggttgtc tccatcccga cggataaaat
     5581 cgccctgcat gatgcaacat tacaggcatt tctggatggt attcctcccc gggacccttc
     5641 agttaagagg ctgaaattca caggtaatgt cccggataca acgatctggc aacacggcga
     5701 tgacctactg gttcgttccc gggcgatgtt gcgcgatgaa tttgaacaga ccttgtcctc
     5761 tgccgacgga actcatctct ggaaacttcc cgtcacccca ctactgactt tctctgtgaa
     5821 tggccagtcc gttcacgtga caccagaact ggagtaatca tgagtgctga acaggatgcc
     5881 ggtaaatccg gaaagaaact ggctgccctg cttggactgg gcagcatcat cttatttggc
     5941 ggcggctata tcgcgttttc caaactatca gggagtaaca gcgatatgca gtcagccgtg
     6001 aacattaaca gtgcagcaag cggcggaacg cgcagcgtta ccgaaacgcc tcactatcgt
     6061 gaactgttaa gagcagataa tgaacgaggg gccgcagctg cagcccgcaa taatcagacg
     6121 ttcattgcca gtcttccaca ggggcttgat attccggact ctcaggaaaa gcaacaacag
     6181 cctgcagcaa aaccggaaaa ttatgctcat cgccaggctt ccggcacacc acaggaggat
     6241 cgagcagcca gtgaaaaacg tatggagcgg ctgcaaaagc tgattgtaag aataaaagat
     6301 cagcatcctg ccggtagtac accaaccata gcaacggcta tgtggaataa atctccggca
     6361 gaaacaacag ggcagaatgg tacgcagcaa ttcgcattac agaatgccag cctgtccacg
     6421 ccggtagcag aaaaagggat acaactgata cctgctctga cccgtatacc ggcatatatt
     6481 gatactgcag tggattctga taaccagtca tccaaggtta tcgcaaccat tccggctggc
     6541 ccctgggcag gggcaacgct gttcagtccg ggagtcaaac tggtgggtaa cggcgtggag
     6601 atccatttcg accggatgag ctggaacggc atggacctta aggtcaatgc atatgctcaa
     6661 cgggaagaca acctgatgtc atccgtggcc agcaatgtga atacacgctg gtttaaacat
     6721 atcattctgc cgtcagtgtt aggaggtgtg ggcagtattg gcacacttta taaagatgcc
     6781 aacacgcagg ttattcaggg taactatggc acggttaccg gacgcgtggg aatgcccagt
     6841 ggtgaagctg ttgcgggcgt tattgccggt ggtatggcgg aaagaggaag tcagatatta
     6901 acccgccagg cagaaagtga gccctataaa caggtagaag tgtatcagca tgaagtcgta
     6961 tcaattctgt ttgttgaccc ggtaatgaca aacgacgcca ggtcatcttc cctttccagt
     7021 ggcatttccc cttcagttaa tcgtacatca cagacggaac aacgctcgca ggcacgaatg
     7081 cagacagcca tggagcaaag aaaagctgtg atgcaacgtc ggtatgatga acaaccggag
     7141 acaccataat gaaacctgaa acagaaatag atgaaaccgg cagcttcggt gaacccgaag
     7201 agaaacccgc ccctttctgg aaacgttccg tctgggggat atctgtagca acatgggggc
     7261 tatgtgcagt cgtattgctc gcagccatat ggtatctgtt cctgagagca ccatcggaaa
     7321 caggcatgcc tccgtttaat gatgcggatg ctggcgtaca gacctggcaa accacacaag
     7381 aatcatcacc ttccgttcag tccactgaga ccatgactgt ccggacagga gaagatatga
     7441 gccagcttgc ccgggatgta aaaacagagc tggataaccg cgatgaaaaa attcaggcaa
     7501 cactgaatat gcttcacgac agcattaaca agctgggcga agctataaaa aaggatgaag
     7561 aatacgcaca ggaaacgcgc cgtcagctgg atgatatccg gagccacctg aacggaatga
     7621 tgacacagaa aagcgtcacc gaatcctctt ccacaccgca tccagcaaag aaaaaaacgt
     7681 cgtctgtact taacggaatg aaaatcatgt cgatggaaac tggcatggtc tggattcgct
     7741 ggcagggtag tacctgggct gtcagagaag gtcagactct gggcaatgtg gtgatccagc
     7801 ggattgatcc gacgacccgg accatcatca ccagtgcagg gacgttacgc tgatgaacat
     7861 ggatgccctg accgccattg agaactttgc cagcagtatt ttcagtgccg gaatggattt
     7921 cctgtttacc tggggagaat tcatcggggt gatcagcatg attaccctgt ttgcccgcgc
     7981 ccgttcagcc ggtccggtaa aaatgtctcc cggaaaattt attgcgggga tgttaaccag
     8041 ctgcatgctc gtatcactcc cggcaatgat taacgccgga ggcgtacaaa tgggattcag
     8101 ggctgactca tttggcccga ttgcatatgt acaacctcag acgtttggcg ctgctgctgg
     8161 cgcagcaaat gcagttctgt cgctggccaa gctggccggt gtgggatttg tcatgaacgg
     8221 gatatccatc tggcgcaagg ccggactcga tggacatacc gcactgagtg ccagtgaatc
     8281 cgtcagtaaa ggaaacgtca aatttattgc cggcgtcctg ctggtcttta tcgatcgtgt
     8341 cctcaatgcc ctgctggcaa gcattggaat cgtcttctga tttttttcac cttcatataa
     8401 accactgata tatccattta aggagaaact atgtcacgta ttcgtgtcgt ggcggcccgt
     8461 ctgtacgccg catttatcca tgcacaactg tttgtaatgt catgccggag caaactgact
     8521 aagtacctgc tgctcattcc catgatgttg ctgcccaaag ccgttcttgc cgatggagat
     8581 cttgccgata tggttcgcaa tgttggtgaa ggtgctaaaa cagcacagtc atcatcaatc
     8641 actattgcgc agttcatcgg cgtaattctg tttctcgggg gcctgattgg cctgaaaaaa
     8701 gttgggaaac aaggggggat gggactggct ggttgcattg tatctatcgt cattggggcg
     8761 gtacttgtcg ccggacctga aatgatgagt cgcagccaga aacagttggg gatttcctca
     8821 atttccattg gctaatcttg tccagggcac tggcatttat ccggtgccct tcctgttcgg
     8881 caatacaagg aggcacatat gtctgcctgg agcccggagc acataacgtt acgacgaaca
     8941 gaaaacggca atgtgattgc ccacgacagt cgggacgatc aggaattcat ttttgcagaa
     9001 tgctgttccg atgaaatcgc aaacatcatt ctggaagccg tgaaacgtta caccagtacg
     9061 gagcctgctc atgcaacaca gcattaaaga cttgtggttg tatccctttc cagaaattga
     9121 tgtggttcat acacaggagc cactgttacc ggaaccggaa ttaacaacgc ccggtcgatg
     9181 tatctgctgc cggcaaaacg tcaggcaccg tttcagactg gatgactcat ggccactcag
     9241 acagcttacc gacacaatca gtgacacacg agttcgtctt aataaggcca cagaacatct
     9301 ggataagtta aaaaagcgtg gtgaaccagt cgcaaccggc gaaaaggaaa agtacaacac
     9361 ggcagtaaaa gccgctgaac gggcgcttga gcaggcccgt ctgtctgcca gacgtctttc
     9421 actccgccat gtacagaaag ctgaaatcac cagtacagag tcgttgtcag agaaagagca
     9481 ggaactgttt catgaagacg gcccgcctta cagcttatgt gctttctgcc acgcctggca
     9541 ttcactgaat ggttatgccg cagcacaggg cgttatggtg tggctacccg accttcatcc
     9601 atccacggtc gtggccctta accgtcgttc attacaggaa gttttcagta atgataagtt
     9661 ccgggtccgg cggggacggg aagcgctctc tgcactgatg caaaacagac tggcggttga
     9721 ggataaattc cgcagtttcc gcccggccga tttcgctgac gtttttcgtc gctatccacc
     9781 ttccggtcgt tcgcccctgc gtgaaaaaat gaacgggatt gccctgattc tgacaccaga
     9841 ttcattcatc aaaaaagaat acgttgattg atttttcttc atgccactcc agcgcggggt
     9901 ggcattaaaa aaatcaaact gcacaaacct ccctcctgta agtccggagt tccctcacct
     9961 atgaacataa accgtttaat cttcactatc gaagactgtc tgaacacgct atcccgtttc
    10021 tctgtcgcaa gcagttttgt tgagtattgc gatttacgta ctgtaattgg tctggacaga
    10081 caggatcgtg aacgtcgccc atggctgaat tcgccctata ttgcagccac aaagcgtgga
    10141 gaatatctct ctgtctttga agtcagcggt gctttcaggg aaatggatga agcatccgac
    10201 cagactggcc taggttcact ggaatcgctg atcaccagca tgagtgacag cctgaatacg
    10261 gcatataaaa acagcggtca taaaatcagc tgcgtttttg agcgtgatcc tgacatgggg
    10321 aaagaagaaa tcgaagacat ggtcgcccca caaaagcgct cactggctaa tacaggtatc
    10381 cagttacagg atgtggtcga tgagaaagtc acaacactgt ccccatggct ggtccgggag
    10441 cgttgctggc tggcgatctg gagtggaccc gaccttatca gcaacagcga tcgcaccgcc
    10501 catgatgagc ttgtcagaag actggctgaa cgggtaccga aagcacgctt tgcccagtcc
    10561 ccctggcagt ggaccttatc tgcactgaaa atccgccacg aagcttttct tgataatgtg
    10621 gaacaggcac tgagacattc atctgatggc ctgattctcc ggctactgga tatacacgag
    10681 gtcgggcgtg aaatacgtcg tcagacagaa cggcacagca caccgcgtaa ctggcaaccc
    10741 cacctgcctg aagatgccca gccagcggga tatcgctgga ctgacgatga atccgtacta
    10801 catgctcctt cattgcattt gcagttattc aacacgcagg tgaccacaca gggcaacctg
    10861 gttcaggcgg gcggactctg gcacggaatg gtatcgatta ctttacctcc gcaaaatctg
    10921 cagacgttta acgaactggt ccgtgccgtc ccccgcgcag tcccctggcg catcagaatg
    10981 gatctgatgc ccggcgggat gaaggctctt aatctgaaga aaacgcttct gacttacagc
    11041 agcttcattt ctgccgttcg tccaatgtat gaatccgtta tgaccctggc ggcaacggat
    11101 gaaaaagaac ctgtctgcat aatgaccatc atggcctcca cgtggggaaa aacacgtgaa
    11161 atatgcacac gaaaccaggc cattctgaaa tcggccattg aaggctgggg cgtatgtggc
    11221 accacgacaa catttggtga tccccgacgc gcctgggtga ataccattct cgcggcatcc
    11281 ggaggctccg gcccggttcc cctgtatccc ccgttgtcac atgccatctc gcttttccca
    11341 cttaacaggg caggctctgt ctggcgaggc aaagggaatc tgatgttaca tacagaagac
    11401 ggttccgcat ttgaggttgg tctggcgtca tcccaacaaa ataaacatac agaactggca
    11461 ccaggcgatc cgggactggg taaatcagtt ctgattaata cgttgtcgga aatacagata
    11521 tcatctgctc agaaaaacct gcccttcatt gcctatatcg ataaaggata cagtgctcag
    11581 ggactggttc agcttattcg cgacagtctt cctccggaaa gaaaagacga agctgtcggt
    11641 attattctga gcaatgatcc ggaatacacc cgtaaccttt ttgacgtgat gtacggcgct
    11701 aaaaagccta ttacgccgga aaagaatttc atgtcatcgg tgctgtgtgc tctctgtgtc
    11761 gataccggta ccggtcaacc ctgtaacccc ggagacaccc gacaaattat caaccagctt
    11821 atcgagctgg cttttaaaga gtacggtgaa aataatccgc gactttatcg cgcctcaacg
    11881 gaagacctgg tggattcagc actgcaggac tcgggactgt atgaaaaaca tgatgcagcc
    11941 tggtgggcca gatcaacctg gttcgaagtc cgggatatgc tccacaatgc cgggtatatc
    12001 atggcggctc agcgggcaca ttatcaggca atgccccagc ttcccgaagt atcctctatg
    12061 ctgggccata ccagtctcag ggatgttttc ggtactgttc agcgtgatgg ttcaaacgaa
    12121 cttctgctgg actacatccg ccgcgccctg gagcagggac ataacgacta ccccatgata
    12181 tccgggtata cccggtttat gatcaacccg gaaacccggg tcatcgctgt cgatctaaat
    12241 aatgttgccg gtgacaaaac gcctgcaggc aggcttaaaa ccggcatcat gtacctgctt
    12301 gcaggtcaga tcgccggtgg tgacttcacc ctgccacaat acagggatga agtgttaaag
    12361 cagcttccac gggaatacca tgaaatcgct cttaaacgta ttaaccaact tgatcaggag
    12421 gttaagacaa aagtttatga tgaactgcat aacgcccggg gcattgactt catctgggaa
    12481 aatctggata cccaggagcg tgagcaacgc aagtttgcta tccgcacggt tctgagtact
    12541 cagtatctga gggactaccc ggaaagtgtt cttaagtcag caaatactct ctggttactg
    12601 cgctacaaac ccgaagatat ccccgtactg agggataact tcaacgtgcc tgaatttatg
    12661 ctgaaacgct tcctgaaaat gccggaaggc ccggcaccgg atggtagtgg tgttcctgtt
    12721 ctgggagtat tcagggtaaa aagtggcacg cttgcgagaa tactcaaatt taccgtcggt
    12781 ccactggagc tatgggcgct gaactcatca ccaaaagaca gtgccctgcg taaaacgctg
    12841 acaaataagt taggttcagt tcgggcacgt aagattctgg ccgaaaactt ccccagaggt
    12901 tctgcaacgt cattaattga gcaccgggcg ggccaacata acagtgacaa tgttattgaa
    12961 gatctggcct cggagctcat ccgaaaacag ggatacaatc tctgatgtgt acaattcata
    13021 tcaccccacg ctgccggaga cctgccagag ggcaggtatt tcttaaaaag cttgaggtaa
    13081 aaggtgacag tttctcattc actctgccgt cacgtctctt caggttacat ccggccccca
    13141 atcccagagt gatatacgct tcggatgcca gaacacaggg aaaggttaca ggcatgcggc
    13201 tgttgttgcc gtccagcaca gatgagctga taacaacggc aatatggcag gtttctgtcc
    13261 cggatgaagc accattcgac gttcagcatg tcatccagtg gatactccct tcaccgctgg
    13321 cttcgctgat gtccgatgat acctggttat ggccatccgt tcccggaacg ccggcactga
    13381 gtgccggtga ctggcctgtt atggatactg acccttccac cttacggcgc gtcagacgtc
    13441 gttctttcag catcatcgat aaccggaaca ggaaaggtta tatcaccagg cgatatcagt
    13501 gtttcccact ggccccacta cctgaagaca gaaaatacga cagccttcct gaccttgtgc
    13561 tccatcccga gcaggagaca ataaaatgca aagaaaaaca ttactcgcag cccttatagc
    13621 caccctgagt ggaacggcat gccaggctca tgcttattca gtcaccgtcg tcgccagccg
    13681 gcctgttgaa gagcaggtca tccccagaat ggaagccatc aaagatgtcc ttggcaacat
    13741 actgagtacc cagacagcca cgggtaccgc tatcaatcag aactctgaaa agctggcaag
    13801 tgtcattgcc cagaacggac aggcgacacg tcagcagatg attttcagta atgaaactca
    13861 gcgcctcgag gaggcacgga aaagcttcac cgtgccagac tccatctgca gtgaatccgc
    13921 atcagggatt gcaacagaaa gcaaaagtgc ctcagcatcc gcagcctcaa aactcagtaa
    13981 aggtggcggt gtgagcaacc gtagcatccg cgaccgtctg gccagcgcag caaactctcc
    14041 cgtgcgggaa gcttatgatg gcgcggccat acacgccagt tactgtacag aagcagaata
    14101 tgcacgtttt ggaggaaccg ccgtttgtcc ttctgtgggc gaaataccag gtggagacag
    14161 tcaggtacgc tcgatttacc acggcgcagg tacagcggac acacctgcag cactgacatg
    14221 ggatcagaag caaatagatg cagcgacggc ttacatgaaa aacacttccc gcccctctgc
    14281 cggtcgtgct ctgggtaaag gagaagtgaa tacccagagt gggcgaacct acgtcggact
    14341 ccagaacgaa tacaacggca tcattgattc tgcgtcgaat ccccaactga cgctgattgc
    14401 tgacagcacg cctaatgaaa gcacacgtaa agcactggct gaaacactgc agtcagactc
    14461 agcggccgct tacttcgacc aggtggcttc accagaagca aaagcacggg gatatatgag
    14521 tacccgtgag tttgaagctt ttgaggctgg ccggcgctac gccaacacag catacctggt
    14581 ggatttacag gaaatgcagg gagataacct gctccgtgaa ctggtaagaa tcactgcgca
    14641 aatgaactgg caactgaacg acctgaaaga acagatccgc cagggtaatg tcatctctgg
    14701 acagcaactg gctctgaccg cccgccagta ttatgaaaaa caactcggta gccttgagaa
    14761 aacaatcaat caggcaaacg cacgttgaaa gctgacgtca gaacaggact gaaaaaatga
    14821 ataagttacc ggaaaattca gaccagacga aaaacgtcaa aaaatcacca tccataaccc
    14881 gcatagccac ccgggggatt attaacattt ttgttcctct gatggagact cgcctgataa
    14941 cgaattctgt acagcatatg ctggaacaac aaaaacgcag gctggacaga tttcgtcagg
    15001 taaataataa aaaggcgcaa ctccacctca cgtgggaaga agctctgcag gccagccata
    15061 tgtcagtaga cgatctggat cgtcgtttcc gccggcgcag aacagtatgg cgattctgtt
    15121 gctggagtct gctggccatc gctttgtttc tcagtggtat gctctttgcc gcctcttcat
    15181 tacctcttac gactctggta cgcgccatca gcactcttgt gttgatactg tcaggcgtcg
    15241 ctctttgtgc ttccagagca ctgattgtta cataccgcct ctggcagttg catgaacgaa
    15301 aagtcagcga acccgagcag gggactttcc gtgattttct gaacgaccgc aatggctggc
    15361 gcaatgccac attaattgcc gtaaccagta aacaatactg atcacgtcca gatgccagta
    15421 ccactggcat gtcgccatcc cccttaatta aatcctgtgt ggcctttgcc catacgcaat
    15481 tctattccgg agttactgtg aaaattttac ttcgcgcact ctgtgcaggc cttgctatat
    15541 cctcactgcc ggcaatggct tcagtgacct atcaggatat tgtgtctgcc gcaaccaatc
    15601 ctgacgatct gtctcgccag gcgctggtca cgatattcgg tgatgtagtc accaatccac
    15661 tgtcaaccag cgcccccact ctgatcggaa gcatgttcgg ggcgttcaac agtatcatcg
    15721 ccgtacttgc cgttgtctgg tttatgttca ttggtatccg tcatgttgtg cgttcggggc
    15781 atcagggaca ggtattcagc accggacgcg atatcgtcgg cacactgagt gtcgttgcag
    15841 gttttctgat gatcgttcca accggaaatg gctggagtat cgctcagctc attatgttat
    15901 ggggcgcatc gataatgggg gttgggtccg ccaatgtgat ggtgcaactt gccgctgata
    15961 atatcgcaaa tggttattca atgactgtac agccagttca ggcatccaca cgaacagcag
    16021 cgcgtggcat ttttgaaatg gaactgtgta agtatgcggt taacgcagga ctaaatgatt
    16081 ttaatcagac ggtcaaatcc tcgacttcac tgatgacaga atcagctaaa accgccagtg
    16141 gtaattacac tgtcacggta tccaatggca gcggtatctg tggttcagcc agcctgtcag
    16201 tagaaggaaa cggaacaaca gatcaatcga cgattggaaa attctttaat cccttcagta
    16261 aaaacgaata cagcggtgtt atctcagcac aacgtgcagc catggataat atgattagcg
    16321 acatggacaa cgctgccagt gagtttgtga caacatttct ggaaaaaaga aattctggca
    16381 acggaacatt gccggatatc gagacacgga ttcagagagc agccgacgag tatgaacgcg
    16441 ctgtacaaaa atcgctacct attgataacg gagaacaatc caggaaagaa gctctcaagt
    16501 cttatctcac aacctatgga tgggtgacgc ttggcgcgtg gtatcagacc tttgccactg
    16561 ctaaccagcg tctggcagag ctggctgaca gagctccagc tgtaacttca atgtcatcac
    16621 ttggggaagt tggcgatact gatttattta gtgctgttat gggggcatac aagactcaat
    16681 tacagaactc cacatatact ccaacgatag gaacgataac aactcaagat gaaagtaatg
    16741 cagctaatag cacagaccca caaagtgtgg tcatgaaaag tatagcgcca accatattaa
    16801 aatggacaaa ccaagctgct actgaatggt caggaacagg aacaaccagc aaccaagtta
    16861 acccattaat taagatgaaa aacatcggcg attatacatt gggtacaaca gaaattctct
    16921 ggacaggata tacaactgtc agagttctcg cgacaatggg tgataactct attttcggaa
    16981 aggctgttaa tttgttctct ggattaccta aaggatttgt tgctctgctg gatgctgcag
    17041 ctcccccaat atatttcttg ttgttcctgt tgttttgtgc gggtttttcc ttatcaattt
    17101 acctaccatt tataccattc attttctgga tgacaggcat tgggaactgg atagtaagcg
    17161 tattaatagg atgcaccgcc gggcctctgt gggcagcaac acatctagga acatcagagg
    17221 acagaggaag tcgagcagct tacggatata tatacttaat tgacagcatg ataagaccac
    17281 ctctcatggt attcggtttc ttttttgcct ctgtcgccat cattgccgtc ggcacaatat
    17341 taaactcact gtttggcgca gcactagtta acgtacaggt aaaatcatta acagggatat
    17401 tcagccttgc cgggttcctg ctaatttacg cccgtatgtg tacaacgtct gtagctgcaa
    17461 tatttgcttt acaagcatat cttcctgacc atgttatcaa tttcttgggt ggtagagatg
    17521 gtgtaaatac tcttggcaac ctcacctcat cagtaaaaga tatttttgca ggttctaata
    17581 gaaatattag acacagccct ggagttagag aggacagact aaaaaatgtc acaaaggatg
    17641 ataataaaga tggaataaaa ggataatttt aataaaacaa ccttgccata aacactcaat
    17701 cgcattaaa
//
LOCUS       DAZOZD010000071        17581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOZD010000071 DAZOZD010000000
VERSION     DAZOZD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.071"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            12..514
                     /locus_tag="ACHUPB_001935"
                     /pseudo
     CDS             12..514
                     /locus_tag="ACHUPB_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUPB_001936"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHUPB_001936"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0758758.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUPB_001937"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHUPB_001937"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0758759.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUPB_001938"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHUPB_001938"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0758760.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUPB_001939"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHUPB_001939"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0758761.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUPB_001940"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHUPB_001940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0758762.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUPB_001941"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHUPB_001941"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0758763.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUPB_001942"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHUPB_001942"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0758764.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUPB_001943"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHUPB_001943"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0758765.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUPB_001944"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHUPB_001944"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0758766.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUPB_001945"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHUPB_001945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0758767.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUPB_001946"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHUPB_001946"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0758768.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUPB_001947"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHUPB_001947"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0758769.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUPB_001948"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHUPB_001948"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0758770.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHUPB_001949"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHUPB_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0758771.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4367 a         4925 c         4570 g         3719 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccaag cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 g
//
LOCUS       DAZOZD010000072        17549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOZD010000072 DAZOZD010000000
VERSION     DAZOZD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.072"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHUPB_001950"
     CDS             <1..139
                     /locus_tag="ACHUPB_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR0758772.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
     gene            97..774
                     /gene="bglJ"
                     /locus_tag="ACHUPB_001951"
     CDS             97..774
                     /gene="bglJ"
                     /locus_tag="ACHUPB_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0758773.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(812..1600)
                     /gene="fhuF"
                     /locus_tag="ACHUPB_001952"
     CDS             complement(812..1600)
                     /gene="fhuF"
                     /locus_tag="ACHUPB_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0758774.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            1741..1977
                     /locus_tag="ACHUPB_001953"
     CDS             1741..1977
                     /locus_tag="ACHUPB_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0758775.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2016..2102)
                     /locus_tag="ACHUPB_001954"
     tRNA            complement(2016..2102)
                     /locus_tag="ACHUPB_001954"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2066..2068),aa:Leu,seq:cag)
     gene            complement(2370..3401)
                     /gene="rsmC"
                     /locus_tag="ACHUPB_001955"
     CDS             complement(2370..3401)
                     /gene="rsmC"
                     /locus_tag="ACHUPB_001955"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0758776.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            3504..3917
                     /gene="holD"
                     /locus_tag="ACHUPB_001956"
     CDS             3504..3917
                     /gene="holD"
                     /locus_tag="ACHUPB_001956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0758777.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            3886..4332
                     /gene="rimI"
                     /locus_tag="ACHUPB_001957"
     CDS             3886..4332
                     /gene="rimI"
                     /locus_tag="ACHUPB_001957"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0758778.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4347..5024
                     /gene="yjjG"
                     /locus_tag="ACHUPB_001958"
     CDS             4347..5024
                     /gene="yjjG"
                     /locus_tag="ACHUPB_001958"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0758779.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5115..6704
                     /gene="prfC"
                     /locus_tag="ACHUPB_001959"
     CDS             5115..6704
                     /gene="prfC"
                     /locus_tag="ACHUPB_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0758780.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7097..7702
                     /gene="osmY"
                     /locus_tag="ACHUPB_001960"
     CDS             7097..7702
                     /gene="osmY"
                     /locus_tag="ACHUPB_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0758781.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            7829..7990
                     /locus_tag="ACHUPB_001961"
     CDS             7829..7990
                     /locus_tag="ACHUPB_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0758782.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8112..9185
                     /locus_tag="ACHUPB_001962"
     CDS             8112..9185
                     /locus_tag="ACHUPB_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0758783.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9182..9964
                     /locus_tag="ACHUPB_001963"
     CDS             9182..9964
                     /locus_tag="ACHUPB_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0758784.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(10480..11343)
                     /locus_tag="ACHUPB_001964"
     CDS             complement(10480..11343)
                     /locus_tag="ACHUPB_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0758785.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(11315..12865)
                     /locus_tag="ACHUPB_001965"
     CDS             complement(11315..12865)
                     /locus_tag="ACHUPB_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0758786.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13123..13902
                     /gene="deoC"
                     /locus_tag="ACHUPB_001966"
     CDS             13123..13902
                     /gene="deoC"
                     /locus_tag="ACHUPB_001966"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0758787.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14029..15351
                     /gene="deoA"
                     /locus_tag="ACHUPB_001967"
     CDS             14029..15351
                     /gene="deoA"
                     /locus_tag="ACHUPB_001967"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0758788.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            15403..16626
                     /gene="deoB"
                     /locus_tag="ACHUPB_001968"
     CDS             15403..16626
                     /gene="deoB"
                     /locus_tag="ACHUPB_001968"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0758789.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            16706..17425
                     /gene="deoD"
                     /locus_tag="ACHUPB_001969"
     CDS             16706..17425
                     /gene="deoD"
                     /locus_tag="ACHUPB_001969"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0758790.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4187 a         4498 c         4746 g         4118 t
ORIGIN      
        1 ggcaattcac agcaacgctg aaatgtatgc atggataaat agcgcgcagg gtgcaagaga
       61 acttaacttg ccttctgttt atggagatgc cgcagaatgg aacacagccg aattaagaag
      121 agaaatgtcg cactcataga aaaatgcgtc atgagcagta tcggtattga gagtttattc
      181 agaaagtttg cgggtaaccc ttataagctc catacctata ccagtcagga gtcatttcag
      241 gatgccatgt cgcggatctc atttgcggcg gtcatttttt ctttttctgc catgagaagt
      301 gagcgcagag agggattatc ttgcctgact gaactggcga ttaagtttcc gcgtacccgg
      361 cgtttagtta ttgcggatga tgatattgaa gctcggctga ttggttcatt gtcgccatca
      421 ccgctggacg gtgtattaag taaagcgtca acgctggaga tttttcatca ggaactcttt
      481 ttgtcattaa atggtgtacg tcaggcgacc gaccgactga acaatcagtg gtacattaac
      541 caaagccgga cgctaagccc gacggagaga gaaatattgc gctttatgtc gcgtggctac
      601 tcaatgacac aaattgccga gcagcttaaa cgcaatatca aaacgatccg tgcacataaa
      661 tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag ggttgttgga ggccgcagat
      721 attctgttat gtatgcggca ttgcgaagca agtaatgtgt tgcatcccta ttaatccgca
      781 tgatgccggg tttacttccc cggcagtgct ttcatttcag cgtacaatcg ccacattgct
      841 gcacatccgg taagcgataa cgctggcagc aggttcggcg caccagcagg ccatcgcgca
      901 gcacgacggt acgccagagt ggattatctt caccgttcgt gagcgttttc tcaaaaaaga
      961 gggcatggcg cagcgattca accgtcgcct cgccgagcag ttgcttcatc tcagtgagat
     1021 accagttgat caaataaccg gtattactcc agataagttt gccgttgatc tctccggtcg
     1081 cttctagtgc ctgcacaacc ggaaccagcg cctggctgat taacgtttcc attcgctgct
     1141 gcggcgaatg tggtgttgcg tttttatctt cacacacatc gacccagaaa caggcgacgc
     1201 gtccggtttc gtgaaactca gcatggaaat gttccggcga cacatctaat gccttttcct
     1261 gcgtcagtag cgccagcatt aatggtggca ccatcaggcc gatatacaat tgtgcccata
     1321 gtgagatcag cggtttgttc tcgcggatca tcgttggttg gttgcgatag atatgatcgg
     1381 aatagaccgc gagtagagaa cttagcgcat tcggtgatga ccattgcgcc agcgtcatgg
     1441 cgttaagcgg agcgggttca tccaggcgga taaactcgag caaatgttca cgatgtttgg
     1501 cgatcgtcgc acgtacggct tgtgcaagcc cagcatcctg cggctggaga tgcgttcgcc
     1561 agatgatatc ttcatagagc ggtgcggaac gataggccat aatcgggata gtaatctaaa
     1621 tgataatgat tgctaatcct agcgataggt ttacccgata gcaagggatt tctctggctt
     1681 gcaaatgata aaaattatca tatgatattg gttatcatta tcaatgaaag agatgaaacc
     1741 atgttgcaac gtacgctggg cagtggctgg ggagtgttgc tgccgggatt gctgattgca
     1801 gggctgatgt atgcggattt atcgccggat cagtggcgga ttgtcattct gatgggatta
     1861 gtattgacgc cggtgatgct gtatcacaaa cagttacggc attacgtttt gctaccatcg
     1921 tgcctggcgc ttattgctgc catcatgctg atgataatga atttgaatca gggatgaaaa
     1981 atcaaggaag aaacaagaaa ggaagtaaag ataattggtg cgaggggggg gacttgaacc
     2041 cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc cgccaccttc
     2101 gcataccatc aattcttaaa aagaattgct accacggagg cgcattctag tggttttcag
     2161 cttttcgtca atagttaatt atcgccggag gtgtaattgc tggaaaaatg tccatcagga
     2221 aactagcgtg caggtttggt aggcatgcgg gggcagatgc cagatgcgac gctggcgcgt
     2281 cttatctggc ctacgaaggg ctaacgtgca ggttttgtag gtcggataag gcgttcacgc
     2341 cgcatccgac acggtattcg gcgagataat taacctttct tcgcctggcg ggtcataatg
     2401 gcgcgataca ccttgaaacg cccggtttgc gcaatcactt catggaagcc aaatgtctca
     2461 tccagcacgt ccgggtaagg caggaaggcg ttcgctacaa ttcgcagctc gccgccgcta
     2521 ttaagatgac gcaccgcgcc gcgaatcagc gtttgcgccg catccaggct ggtttgcatc
     2581 ccatcgtgga acggcgggtt ggagatgatc atatcaaaac gacctttcac ctcggaaaag
     2641 acgttgctgg caaagacttc accttcaaca cagttggccg caagtgttgc gcggctggct
     2701 tctaccgctg gcgcagagac atcgcacaag gtgagacgaa ttttcggcga atggcgcgca
     2761 aaggcaactg aaagcacccc cgcgccacag ccgacatcca gcactttacc tttcgtgtgc
     2821 ggagttaacg tcgagagcag caactggcta ccgacatcca gaccgtcgcg gctaaacacg
     2881 ccaggcagcg ttttgaccgt caggccatcg acgctgtatt cgccccagaa tttatcggca
     2941 tcaaataccg gctgtttttc cagacgacca aaatagaggc cacagcgacg agcgctgtcg
     3001 actttattca acggcgcata atctgccagc atctgctcgg cgctgcgcac gccgctgcgg
     3061 ttctcgccaa cgacaaaaat atctgtcccc actggcagca gagaaagtaa attcatcaac
     3121 tggaactggg cttccggttt gttcttcggc cagtagtaaa tcagcatatc gcaatctgcg
     3181 acgtcatcca ccgtggcgac cagactgaaa cgggcgttat cccccatctg gcggcttaat
     3241 acctgccagt ggtggaattg ctgggtatga gcacggctgg ccgcggtatc taaacgcgcg
     3301 ggcaggtcat cctgtaagtc tccggcaaac agaatacggc tttgttcgaa atcatcactg
     3361 tgacgcagca agacttcact tgccggggta aatgcagaca tggaatgctc ctcaattgat
     3421 actggcggcg attatagcca tatgttggcg cggtatcgac gaatttgcta tatttgcgcc
     3481 cctgacaaca ggagcgattc gctatgacat cccgacgaga ctggcagtta cagcaactgg
     3541 gcattaccca gtggtcgctg cgtcgccctg gcgcgttgca gggggagatt gccattgcga
     3601 tcccggcaca cgtccgtctg gtgatggtgg caaacgatct tcccgccctg accgatcctt
     3661 tagtgagcga tgttctgcgc gcattaaccg tcagccccga tcaagtgctg caactgacgc
     3721 cagaaaaaat cgcgatgctg ccgcaaggca gtcgctgcaa cagctggcgg ttgggtactg
     3781 acgaaccgct atcactggaa ggcgctcagg tggcatcacc ggcgctcacc gaattacggg
     3841 caaacccaac ggcacgcgcc gcgttatggc aacagatttg cacatatgaa cacgatttct
     3901 tccctcgaaa cgactgattt accagcggct taccacattg aacaacgcgc ccacgccttt
     3961 ccgtggagtg aaaaaacctt tgccagcaac cagggcgagc gttatctcaa ctttcagtta
     4021 acgcaaaacg gcaaaatggc ggcgtttgcg attacgcaag tggtgctgga tgaagctaca
     4081 ttgttcaata ttgcggtcga tcctgactat cagcgtcagg gattgggaag ggtgctgctg
     4141 gaacatctga tcgacgaact ggaaaaacgc ggcgtggcga cactatggct ggaagtccgt
     4201 gcctcaaatg ctgccgccat tgccctgtac gaaagtttag gctttaacga ggcgacgatt
     4261 cgccgcaatt actaccccac cacggacggt cgcgaagacg ccatcatcat ggcgttgcca
     4321 atcagtatgt aagacaaggt ggaataatga agtgggactg gattttcttt gatgccgatg
     4381 aaacgctgtt tacctttgac tcattcaccg gcctgcagcg gatgtttctt gattacagcg
     4441 tcacctttac cgctgaagat tttcaggact atcaggccgt taacaagcca ctgtgggtgg
     4501 attatcaaaa cggcgcgatc acttcattac agcttcagca cgggcgtttt gagagctggg
     4561 ccgaacggct gaaagttgaa gcaggcttgc ttaacgatgc ctttattaat gcgatggcgg
     4621 aaatctgcac gccgctgccg ggcgcggttt ctctgcttaa cgccattcgt ggcaacgcca
     4681 aaatcggcat catcaccaac ggctttagcg ccttgcagca agtgcgtctg gaacgcacgg
     4741 gcctgcgtga ttacttcgat ttgctggtga tttccgaaga agttggcgtt gccaaaccga
     4801 ataagaaaat tttcgattat gcgctggaac aggcgggcaa tcctgaccgt tcacgcgtgc
     4861 tgatggttgg cgacactgcc gagtccgata ttctcggtgg catcaacgcc gggcttgcga
     4921 cctgctggct gaatgcgcac aatcgcgagc aaccagaagg catcgcgccc acctggaccg
     4981 tttcttcgtt gcacgaactg gagcagctcc tgtgtaaaca ctgattgcct cccccccgtt
     5041 gatgggtaaa atagccgcaa tttttcgttt tcaacaagcg cggcgcgatg ccgcttactc
     5101 aagaagaaag aattatgacg ttgtctcctt atttgcaaga ggtggcgaag cgccgcactt
     5161 ttgccattat ttctcacccg gatgccggta agactaccat caccgagaag gtgctgctgt
     5221 tcggacaggc cattcagacc gccggtacag taaaaggccg tggttccaac cagcacgcta
     5281 agtcggactg gatggagatg gaaaagcagc gtgggatctc cattactacg tctgtgatgc
     5341 agtttccgta tcacgattgc ctggttaacc tgctcgacac cccggggcac gaagacttct
     5401 cggaagatac ctatcgtacc ctgacggcgg tggactgctg cctgatggtt atcgacgctg
     5461 caaaaggtgt tgaagatcgt acccgtaagc tgatggaagt tacccgtctg cgcgacacgc
     5521 cgatcctcac ctttatgaac aaacttgacc gtgatatccg cgacccgatg gaactgctcg
     5581 atgaagttga gaacgagctg aaaatcggct gcgcaccgat cacctggccg attggctgcg
     5641 gcaagctgtt taaaggcgtt taccaccttt ataaagacga aacctatctc tatcagagcg
     5701 gtaaaggcca cacgattcag gaagtccgta ttgttaaagg gctgaataac ccggatctcg
     5761 acgctgcggt tggtgaagat ctggcacagc agctacgtga cgaactggaa ctggtgaaag
     5821 gcgcgtctaa cgagttcgac aaagaattgt tccttgcggg cgaaatcact ccggtattct
     5881 tcggtactgc gctgggtaac ttcggcgttg atcatatgct ggatggcctg gtggagtggg
     5941 ctcctgcgcc gatgccgcgt cagactgata cccgtaccgt agaggcgagc gaagacaaat
     6001 ttaccggctt cgtatttaaa attcaggcca acatggaccc gaaacaccgc gaccgcgtgg
     6061 cgtttatgcg cgtagtatcc ggtaaatatg aaaaaggcat gaagctgcgc caggtgcgta
     6121 ctgcgaaaga tgtggttatc tccgacgcgc tgacctttat ggcgggcgac cgttcgcacg
     6181 ttgaagaagc gtatccgggc gatatcctcg gcctgcacaa ccacggcact attcagatcg
     6241 gcgacacctt tacccagggt gagatgatga agttcaccgg tattccgaac ttcgcaccag
     6301 aactgttccg tcgtatccgc ctgaaagatc cgctgaagca aaaacagctg ctcaaagggc
     6361 tggtacagct ttccgaagag ggcgcggtgc aggtgttccg tccgatctcc aacaacgatt
     6421 tgatcgttgg tgctgttggt gtgctgcagt ttgatgtggt ggtatcgcgc ctgaagagcg
     6481 aatacagcgt tgaagcagta tatgaatcag tcaacgttgc cactgcccgc tgggtagaat
     6541 gtgcggacgc gaagaaattc gaagagttca agcgtaagaa cgaaagccaa ctggcgcttg
     6601 atggcggcga taacctcgct tacatcgcta ccagcatggt caacctgcgc ctggcgcagg
     6661 aacgttatcc cgacgttcag ttccaccaga cccgcgagca ttaattcttg cctccagggc
     6721 gcggtagccg ctgcgccctg tcaatttccc ttccttatta gccgcttacg gaatgttctt
     6781 aaaacattca cttttgctta tgttttcgct gatatcccga gcggtttcaa aattgtgatc
     6841 tatatttaac aaagtaatga catttctgac ggcgttaaat accgttcaat gcgtagatat
     6901 cagtatctaa agccgtcgat tgtcattcta ccgatattaa taactgattc agaggctgta
     6961 atggtcgtta ttcatcaccc atcgcttttg tgatggcgac cattgacttc tgtagagggt
     7021 gaagtctctc cctattcagc aatgcaacct cgtgttgcca ggctcaaatt acgagcaaac
     7081 atacaggaat aaatcgatga ctatgacaag actgaagatt tcgaaaactc tgctggctgt
     7141 aatgttgacc tctgccgtcg cgaccggctc tgcctacgcg gaaaacaacg cacagactac
     7201 caatgaaagc gcagggcaaa aagtcgatag ctctatgaat aaagtcggta atttcatgga
     7261 tgacagcgcc atcaccgcga aagtgaaggc ggcgctggtg gatcatgaca acatcaagag
     7321 caccgatatc tctgtcaaaa ccgatcaaaa agtcgtgacc ctgagcggtt tcgttgaaag
     7381 ccaggctcag gccgaagagg cagtgaaagt ggcgaaaggc gttgaagggg tgacctctgt
     7441 cagcgacaaa ctgcacgttc gcgacgctaa agaaggctct gtgaagggct acgcgggtga
     7501 caccgccacc accagtgaaa tcaaagccaa actgctggcg gacgatatcg tcccttcccg
     7561 tcatgtgaaa gttgaaacca ccgacggcgt ggttcagctc tccggtaccg tcgattccca
     7621 ggcacagagc gaccgtgctg aaagtatcgc caaagcggta gatggtgtga aaagcgttaa
     7681 aaatgatctg aaaactaagt aattcgtcgt aattcgtcct cccgaacttt gtcgggaggc
     7741 gcgatgtgca ccacactaaa aatatcgcat tgagtgattt tcacgctcat atcaagcggt
     7801 gacattaact atggtaaagg agacgcttat gtttcgttgg ggcatcatat ttctggttat
     7861 cgcgttaatc gccgccgcac ttgggtttgg tggtctggcc ggtaccgctg caggcgcagc
     7921 taaaattgtc tttgtcgtcg ggattattct gttcctggtg agtttgttca tgggccgaaa
     7981 acgaccctag atttcagacc attaagaaaa ttttatccaa agccagtcca gcggactggc
     8041 ttttgcggtt ttagcgaata ataaattgcg ttactttgtc attctttcac aacaaaaaca
     8101 ggaaggcaga ggtggggcag cgaatacctg taacgcttgg taatattgcg ccgttgtcgc
     8161 taaggccgtt ccagcctgga cgaatagctc tggtgtgcga aggcggcgga cagcgtggaa
     8221 ttttcacggc tggcgtgctg gatgagttta tgcgcgcgca gtttaatcct ttcgatcttt
     8281 atctcggcac atctgccggg gcgcagaacc tctcggcgtt tatctgcaat cagcccggtt
     8341 acgcgcgcaa agtcatcatg cgctatacca caaaacgcga atttttcgat ccattgcgct
     8401 ttgtccgtgg aggaaatctt atcgatctcg actggctggt ggaggccacc gcaagccaga
     8461 tgccgttgca aatggatacc gccgcgcggt tgtttgacag cggcaaatcg ttttatatgt
     8521 gcgcctgtcg tcaggatgac tacgcgccta attacttttt accaaccaaa caaaactggc
     8581 tggatgtgat tcgcgcctcc agtgcgatac ctggctttta tcgtagcgga gtgtcgctgg
     8641 aaggcattaa ctacctggat ggcgggatca gtgatgcgat tccggttaaa gaggcggcaa
     8701 ggcagggcgc taaaacgttg gtcgtcattc gcactgtgcc gtcacaaatg tactacacgc
     8761 cgcagtggtt caaacgcatg gaacgctggc tgggtgacag tagcctgcag ccgctggtca
     8821 atctggtgca gcatcatgaa accagctatc gtgacattca gcaatttatt gagaaaccac
     8881 cgggcaagct gcggatattc gaaatttatc cgccgaagcc attacatagt atcgcgcttg
     8941 gcagtcggat tccggcgctg cgtgaagact ataaacttgg gcgtttatgc ggtcgttatt
     9001 tcctcgccac ggttggcaag ctattaactg aaaaagcgcc gcttacccgc catctggtgc
     9061 cagtggtgac gccggaatcg atcgttattc cgcctgcgcc agtcgccaac gatacgctgg
     9121 ttgccgaagt gagcgatgct ccgcaggcga acgacccgac atttaacaat gaggatctgg
     9181 cttgatttgc cgttttatcg atacccactg ccattttgat ttcccgccgt ttagtggcga
     9241 tgaagaggcc agcctgcaac gcgcggcaca agcgggcgta ggcaagatca ttgttccggc
     9301 gacagaggcg gaaaattttg cccgcgtgca ggcgttagcg gaaaaatatc aaccgctgta
     9361 tgccgcattg gggttgcatc cggggatgtt ggaaaaacat agcgatgtgt ctcttgatca
     9421 gctacagcag gcgctggaaa ggcgtccggc gaaggtggtg gcggtggggg agatcggtct
     9481 ggatctcttc ggcgacgatc cgcaatttga gaggcagcag tggttactcg acgaacagct
     9541 gaaactggcg aaacgctacg atctgccggt gatccttcat tcccggcgta tacacgacaa
     9601 actggcgatg catcttaaac gtcacgattt accgtgcact ggcgtggttc acggtttttc
     9661 cggcagcctg caacaggccg agcggtttgt acagctgggc tacaaaatcg gcgtaggcgg
     9721 tactatcacc tatccacgcg ccagtaaaac ccgcgatgtc atcgcaaaat taccgctggc
     9781 atcgttgttg ctggaaaccg acgcgccgga tatgccgctc aacggctttc aggggcagcc
     9841 taaccgaccg gaacaggctg tgcgggtgtt tgacgtgctt tgcgagttgc gcccggaacc
     9901 agaagatgag attgccgaag tgttgcttaa taatacgtat gcgttgttta gcgttagtgg
     9961 gtagcctacc cttcgtaggc agcatccggc agtcggcgca taatgcctga tgcgacgctt
    10021 aacgcgtctt atcatgccta caggtttgtg ccgaaccgta ggccggataa ggcgttcacg
    10081 ccgcatccgg cagttggcgc acaatgcctg atgcgacgct tgacgcgtct tatcatgcct
    10141 actggtttgt gccgaaccgt aggccggata aggcgttcac gccgcatccg gcagttggcg
    10201 cacaatgcct gatgcgacgc ttgacgcgtc ttatcatgcc tacaagtctg tgccgaaccg
    10261 taggccggat aaggcgttca cgccgcatcc ggcagttggc gcacaatgcc tgatgcgacg
    10321 ctgacgcgtc ttatcatgcc tacaagtctg tgccgaaccg taggccggat aaggcgttca
    10381 cgccgcatcc ggcagtcggc gcataatgcc tgatgcgacg cttgtcgcgt cttatcatgc
    10441 ctacaagtct gtgccgaacc gtaggccgta tccggcatgt cacaaataga gcgccggaaa
    10501 tatcaaccgg ctcaccccgc gcacctttaa cgcatcagcc aacggctcaa cgtcttccgg
    10561 cgtggcgctc gcccagcttt gcgcctcgcc atacacgccg tgggcatgaa acgcattcag
    10621 gcgaaccgga acatcgccaa gtctcttgat aaacgtcgcc agctcatcga tgtgatgcaa
    10681 ataatccacc tggtctggaa ttaccagcaa acgcagttcc gccagcttgc cgcgctctgc
    10741 aagcaaacag atgctgcgct taatctgctg attatcgcgt ccggttaagt gttgatgaca
    10801 ttcgctcccc cacgctttga gatcgagcat tgcgccgtca cacaccggga gtaatttttc
    10861 ccagccggtt tcgctcaaca tgccgttact gtccaccagg caggtgaggt ggcgcagttg
    10921 cggatcgttt ttgatagcag taaacagcgc cactacaaac ggcagttggg tcgtggcttc
    10981 gccgccgctt accgttatcc cttcaataaa cagcaccgct ttgcggacat ggctaagcac
    11041 ttcgtccacg ctcatggatt gcgccattgg cgtggcatgt tgcggacacc tcttcaggca
    11101 ggtatcacac tgctcgcaaa ccacagcgtt ccacaccact ttgccgtcaa caatctgcaa
    11161 cgcctgatgc ggacactgtg gcacgcactc cccacagtca ttgcaacgtc ccatcgtcca
    11221 cggattgtga cagtttttgc agcgcagatt gcagccctgc aaaaacagag ccagacgact
    11281 gcctggcccg tcaacgcagg agaaggggat aatcttactg actaaagcgc atctgctgtt
    11341 catggcttat cacgcgcggc tggcgttcca gaatacgagt gttgcgtgcg gcttcttcgc
    11401 ccagccaggt ggtgttggtg cgtgaacctt cggcgcgata tttttctaaa tccgacaaac
    11461 gcaccatata accggtaacg cgaaccagat cgttaccgct gacattggca gtaaattcac
    11521 gcattccggc tttaaaggca ccgaggcaaa gctgtaccag cgcctgcgga ttacgtttga
    11581 tggtttcgtc gagcgtcaga atgtcgctga tgccggaata ataataagca tgatgcgggg
    11641 cgacagtttg cagatgggtg atcggatctg gctcatcacc atacggcaaa cgcgcgcccg
    11701 gcgtggtgcc gatatcggaa ctgctccccg actgtgcgtg taacatggcg cgtttttgcc
    11761 agccatattt cacgggggta ttggcgacaa actccgccag ttgcgcgctg atgcgataac
    11821 ccacttcatt tgcggcggct tctttaccgt agcgcgcggc aatcccttct ttcttacaca
    11881 gcaagttaac cgcttccgcc agcccataca tgccaaacat tggcacaaaa cgttcagggt
    11941 taatcagccc ttctttcacc aggaagctat tctcaaagaa gtgtgattgt tgatagagga
    12001 attcacaccg cgcatcgatg atggcgatct gctgctggca gtagtgcggt agagtgcgcg
    12061 taaagaagtc atccagcgat tcgctgcgct cggcaatggc tttcaggtta aggcgtacca
    12121 gcgtgctgcc accacccgcc agcggcagtg agttgtaaca gctcacaatc ccgtagcccc
    12181 cttttgtgaa aattttatca tgcaccggac cgttggcgat gtgcggtttg ctacattcac
    12241 agatgttctt cgccacttcc agcagcaggt catcaggggt gatttcagga tcgtagataa
    12301 aggtcaggtt cggtgaaacc tgcttcaact ctgcgtctgc acgtaagatc gcgcgggtaa
    12361 tgggcgaatc agacgggccg atattggcgt gcataaaggc gtctggcagg gttctgtcga
    12421 ggtaacgcca gaaacgtttt attcgaacat cgatctcgtc ttgtgttaga attctaacat
    12481 atggttgcaa caacgcatcc agttgcccca ggtagaccgg catcgatgtg accgacggta
    12541 cgtggtggta aagaatggtc agcagagaga gtgcgtcatc aagatctttc gcgccttcca
    12601 gctccagcca ttcggaaccg ttcgccagaa aacgggcgta atcgggtaag acatagcgcg
    12661 gtttgtacgg cgcatgacct tcaaacatat cgcagattac accctcatcc agcgcgcggc
    12721 gggcttcggc aggaagctgc ggataaggca gattgttttc tgcttccagt gccagaaaat
    12781 ggcgcttctg ctccgggcta agcactgggc tggtgacaat ttgctggcaa cgttgttgca
    12841 gtgcattttc atgagaagtg ggcatcttct tttcctttta tgccgaaggt gatgcgccat
    12901 tgtaagaagt ttcgtgatgt tcactttgat cctgatgcgt ttgccaccac tgacgcattc
    12961 atttgaaagt gaattatttg aaccagatcg cattacagtg atgcaaactt gtaagtagat
    13021 ttccttaatt gtgatgtata tcgaagtgtg ttgcggagta gatgttagaa tactaacaaa
    13081 ctcgcaaggt gaattttatt ggcgacaagc caggagaatg aaatgactga tctgaaagca
    13141 agcagcctgc gtgcactgaa attgatggac ctgaccaccc tgaatgacga cgacaccgac
    13201 gagaaagtaa ttgctctgtg tcatcaggcc aaaaccccgg tcggcaatac cgccgctatc
    13261 tgtatctatc ctcgctttat cccgattgct cgcaaaacgc tgaaagagca gggcaccccg
    13321 gaaatccgaa tcgctacggt aaccaacttc ccacacggta acgatgacat cgaaatcgcg
    13381 ctggcagaaa cccgtgcggc aatcgcctac ggtgctgatg aagttgacgt tgtgttcccg
    13441 taccgcgcgc tgatggcggg taacgagcag gttggttttg acctggtgaa agcctgtaaa
    13501 gaggcttgcg cggcagcgaa tgtactgctg aaagtgatca tcgaaaccgg cgaactgaaa
    13561 gacgaagcgc tgatccgtaa agcgtctgaa atctccatca aagcgggtgc ggacttcatc
    13621 aaaacctcta ccggtaaagt ggctgtgaac gcgacgccgg aaagcgcgcg catcatgatg
    13681 gaagtgatcc gtgatatggg cgtagaaaaa accgttggtt tcaaaccagc gggcggcgtg
    13741 cgtactgcgg aagatgcgca gaaatatctc gccatcgcag atgaactgtt cggtgctgac
    13801 tgggcagatg cgcgtcacta ccgctttggt gcttccagcc tgctggcaag cctgttgaaa
    13861 gcgctgggcc acggtgacgg taagagcgcc agcagctact aagtaagatg ctttacgcct
    13921 gatgcgctgc gcttatcagg cctacgagac gtatctaccc gtaggccgga taaggcgtag
    13981 acgcatccgg caaaagccgc ctcatactct tttcctcggg aggttacctt gtttctcgca
    14041 caagaaatta ttcgtaaaaa acgtgatggt catgcactga gcgatgaaga aattcgtttc
    14101 tttatcaacg gcattcgcga caacactatc tccgaagggc agattgccgc cctcgcgatg
    14161 accattttct tccacgatat gacaatgcct gagcgtgtct cgctgaccat ggcgatgcga
    14221 gattcaggaa ccgttctcga ctggaaaagc ctgcatctga atggcccgat tgttgataaa
    14281 cattccactg gtggcgtcgg cgatgtgact tcgctgatgt tggggccgat ggtcgcagcc
    14341 tgcggcggct atattccgat gatctctggt cgcggcctcg gtcatactgg cggtacgctc
    14401 gacaaactgg aatccatccc tggcttcgac attttcccgg atgacaaccg tttccgcgaa
    14461 attattaaag acgtcggcgt ggcgattatc ggtcagacca gctcactggc tccggcggat
    14521 aaacgtttct acgcgacccg tgatattacc gcaaccgtgg actccatccc gctgatcacc
    14581 gcctctattc tggcgaagaa acttgcggaa ggtctggacg cgctggtgat ggacgtgaaa
    14641 gtgggtagcg gcgcgtttat gccgacctac gaactctctg aagcccttgc cgaagcgatt
    14701 gttggcgtgg ctaacggcgc tggcgtgcgc accaccgcgc tgctcaccga catgaatcag
    14761 gtactggcct ccagtgcagg taacgcggtt gaagttcgtg aagcggtgca gttcctgacg
    14821 ggtgagtatc gtaacccgcg tctgtttgat gtcacgatgg cgctgtgcgt ggagatgctt
    14881 atctccggca aactggcgaa agatgacgcc gaagcgcgcg cgaaattgca agcagtgctg
    14941 gacaacggta aagcggcaga agtctttggt cgtatggtag cggcacaaaa aggcccaacc
    15001 gacttcgttg agaactacgc gaagtatctg ccgacagcga tgctgacgaa agcagtctat
    15061 gctgataccg aaggttttgt cagtgaaatg gatacccgcg cgctggggat ggcagtggtt
    15121 gcaatgggcg gcggtcgtcg tcaggcatct gacaccattg attacagcgt cggctttact
    15181 gatatggcgc gtttgggcga ccaggtagac ggtcagcgtc cgctggcggt tatccacgcg
    15241 aaagacgaaa acagctggca ggaagcggcg aaagcggtga aagcggcaat taaacttgcc
    15301 gataaagcac cggaaagcac accaactgtc tatcgccgta tcagcgaata acggtatact
    15361 gatctgatca tttaaatttg aagcactgag tacggagaac atatgaaacg tgcatttatt
    15421 atggtgctgg actcattcgg catcggcgct acagaagatg cagaacgctt tggtgacgtc
    15481 ggggctgaca ccctgggtca tatcgcagaa gcttgtgcca aaggcgaagc tgataacggt
    15541 cgtaaaggtc cgctcaatct gccaaatctg acccgtctgg ggctggcgaa agcacacgaa
    15601 ggttctaccg gtttcattcc ggcgggaatg gacggcaacg ctgaagttat cggcgcgtac
    15661 gcatgggcgc acgaaatgtc atccggtaaa gataccccgt ctggtcactg ggaaatagcc
    15721 ggtgtcccgg ttctatttga gtggggatat ttctccgatc acgaaaacag cttcccgcaa
    15781 gagctgctgg ataaactggt tgaacgcgct aatctgccgg gttacctcgg taactgccac
    15841 tcttccggta cggtcattct ggatcagctg ggcgaagagc acatgaaaac cggcaagccg
    15901 attttctata cctccgctga ctctgtgttc cagattgcct gccacgaaga aacctttggc
    15961 ctggataaac tctacgaact gtgcgaaatc gcccgtgaag agctgaccaa cggcggctac
    16021 aacatcggtc gtgttatcgc tcgtccgttt atcggtgaca aagccggtaa cttccagcgt
    16081 accggtaacc gtcacgacct ggctgttgag ccgccagcac cgaccgtgct gcagaaactg
    16141 gttgatgaaa aacacggtca ggtggtttct gtcggtaaaa ttgcagacat ctacgccaac
    16201 tgcggtatca ccaagaaagt gaaagcaact ggcctggacg cgctgtttga cgccaccatc
    16261 aaagagatga aagaagcggg tgataacacc atcgtcttca ccaacttcgt tgacttcgac
    16321 tcttcctggg gccaccgtcg cgacgtcgcc ggttatgctg tgggtctgga gctgttcgac
    16381 cgccgtctgc cggagctgat gtctctgctg cgcgatgacg acatcctgat cctcactgct
    16441 gaccacggtt gtgatccgac ctggaccggt actgaccaca cgcgtgaaca cattccggta
    16501 ctggtttacg gcccgaaagt aaaaccgggc tcactgggtc accgtgaaac cttcgcggat
    16561 atcggccaga ctctggcaaa atattttggt acttctgata tggaatatgg caaagccatg
    16621 ttctgataat gggtgcggct tgatgccctc accccggccc tctccctcag ggagagggtg
    16681 aaaacatgtt gataaggata aaacaatggc taccccacac attaatgcag aaatgggcga
    16741 tttcgctgac gtagttttga tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac
    16801 tttccttgaa gatgcccgtg aagtgaacaa cgttcgcggt atgctgggct tcaccggtac
    16861 ttacaaaggc cgcaaaattt ccgtaatggg tcacggtatg ggtatcccgt cctgctccat
    16921 ctacaccaaa gaactgatca ccgatttcgg cgtgaagaaa attatccgcg tgggttcctg
    16981 tggcgcagtt ctgccgcacg taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac
    17041 cgattccaaa gttaaccgca tccgttttaa agatcatgac tttgccgcta tcgctgactt
    17101 tgacatggtg cgtaacgcgg tagacgcggc taaagcactg ggcattgatg ctcgcgttgg
    17161 taacctgttc tccgctgacc tgttctactc tccggacggc gaaatgttcg acgtgatgga
    17221 aaaatacggc atcctcggcg tggaaatgga agcggctggt atctacggcg tcgctgcaga
    17281 atttggcgcg aaagccctga ccatctgcac tgtgtctgac cacatccgca ctcacgagca
    17341 gaccactgcc gctgagcgtc agaccacctt caacgacatg atcaaaatcg cactggaatc
    17401 cgttctgctg ggcgataaag agtaattgtg tttcgctgaa aggcgattgt cgtgttaagc
    17461 cggagtgggg gactgctccg gctttttagt atctattcat caatagcaac tgatttatag
    17521 atttactatt atttttcata tggataaaa
//
LOCUS       DAZOZD010000073        17377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOZD010000073 DAZOZD010000000
VERSION     DAZOZD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.073"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            34..1010
                     /gene="ybeQ"
                     /locus_tag="ACHUPB_001970"
                     /pseudo
     CDS             34..1010
                     /gene="ybeQ"
                     /locus_tag="ACHUPB_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1080..1562)
                     /locus_tag="ACHUPB_001971"
     CDS             complement(1080..1562)
                     /locus_tag="ACHUPB_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0758791.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1797..4379
                     /gene="leuS"
                     /locus_tag="ACHUPB_001972"
     CDS             1797..4379
                     /gene="leuS"
                     /locus_tag="ACHUPB_001972"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0758792.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4394..4975
                     /gene="lptE"
                     /locus_tag="ACHUPB_001973"
     CDS             4394..4975
                     /gene="lptE"
                     /locus_tag="ACHUPB_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0758793.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4975..6006
                     /gene="holA"
                     /locus_tag="ACHUPB_001974"
     CDS             4975..6006
                     /gene="holA"
                     /locus_tag="ACHUPB_001974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0758794.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            6008..6649
                     /gene="nadD"
                     /locus_tag="ACHUPB_001975"
     CDS             6008..6649
                     /gene="nadD"
                     /locus_tag="ACHUPB_001975"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0758795.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6673..7284
                     /locus_tag="ACHUPB_001976"
                     /pseudo
     CDS             6673..7284
                     /locus_tag="ACHUPB_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7544..7861
                     /gene="rsfS"
                     /locus_tag="ACHUPB_001977"
     CDS             7544..7861
                     /gene="rsfS"
                     /locus_tag="ACHUPB_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0758796.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7865..8332
                     /gene="rlmH"
                     /locus_tag="ACHUPB_001978"
     CDS             7865..8332
                     /gene="rlmH"
                     /locus_tag="ACHUPB_001978"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0758797.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8363..10264
                     /gene="mrdA"
                     /locus_tag="ACHUPB_001979"
     CDS             8363..10264
                     /gene="mrdA"
                     /locus_tag="ACHUPB_001979"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0758798.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10267..11379
                     /gene="mrdB"
                     /locus_tag="ACHUPB_001980"
                     /gene_synonym="rodA"
     CDS             10267..11379
                     /gene="mrdB"
                     /locus_tag="ACHUPB_001980"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0758799.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11390..12478
                     /gene="rlpA"
                     /locus_tag="ACHUPB_001981"
     CDS             11390..12478
                     /gene="rlpA"
                     /locus_tag="ACHUPB_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0758800.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12617..13828
                     /gene="dacA"
                     /locus_tag="ACHUPB_001982"
     CDS             12617..13828
                     /gene="dacA"
                     /locus_tag="ACHUPB_001982"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0758801.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13938..14201
                     /gene="ybeD"
                     /locus_tag="ACHUPB_001983"
     CDS             13938..14201
                     /gene="ybeD"
                     /locus_tag="ACHUPB_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0758802.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14302..14943
                     /gene="lipB"
                     /locus_tag="ACHUPB_001984"
     CDS             14302..14943
                     /gene="lipB"
                     /locus_tag="ACHUPB_001984"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0758803.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15203..16156
                     /locus_tag="ACHUPB_001985"
     CDS             15203..16156
                     /locus_tag="ACHUPB_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0758804.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16365..17330
                     /gene="lipA"
                     /locus_tag="ACHUPB_001986"
     CDS             16365..17330
                     /gene="lipA"
                     /locus_tag="ACHUPB_001986"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0758805.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4337 a         4390 c         4458 g         4192 t
ORIGIN      
        1 atatgtttta tctacaacca taaggaaatg ataatggttt ttacgtcaag ttgctgcgat
       61 aatttatcaa tagatgagat tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat
      121 attgttgggt tttattataa tcgcgatagc gcaattgact ctccggacga cgaaaaggcc
      181 ttttactggc tgaagctggc cgctgagcaa ggtcattgtg aagcacagta ttccttaggg
      241 caaaagtata ccgaggataa aagccgtcat aaagataatg agcaagccat cttctggctg
      301 aaaaaagctg ccctacaagg ccatactttc gcttccaacg cccttggctg gacactggat
      361 cgtggagaag cccccaatta taaagaagcg gttgtctggt atcagatagc cgcggagagc
      421 ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa cggagtcgca
      481 aaagactatg cgctggcatt tttctggtac aaacaagctg cattacaagg ccatagtgac
      541 gcgcaaaaca atctggccga tctttatgaa gacggaaaag gcgttgctca aaacaagaca
      601 ctcgccgcat tctggtattt gaaaagcgca cagcagggta atcggcacgc ccagtttcaa
      661 attgcatggg attataacgc tggcgaaggg gtggaccagg actataagca agcgatgtac
      721 tggtatctga aggctgccgc tcaggaaagc gtcggcgctt acgtcaacat cggttatatg
      781 tataaacacg gacaaggcgt tgagaaagat tatcaggctg cctttgaatg gtttacgaaa
      841 gccgctgaat gcaatgacgc cactgcctgg tataacctgg ccattattat cactacggag
      901 aaggaagacc tgtcgatctc cgacaggctc tcgacctgta tcgtaaagtt cagtcatccg
      961 gaaccagaga tgtcagtcag gaaattcgtg agactgaaga tttactgtag agataaaaac
     1021 aagggccggg gaatttcatt cggcccatat tctctttatt gctgtgcgaa atcagactat
     1081 tacggctcaa acgggcgtct ctggaactgg tcatgaccac atttcgggca tagcgtcagc
     1141 acttccggtg tgtagatcgg gagatggaag tgacatttct cgcagaccag atttcccagc
     1201 ccgaccactt ctccgctgtg ataaacccca tgatgattga ggtcctggaa aacttcgcgc
     1261 cattcaagct gcgttttatc ggtgatatct gccagctcct gccataagct ttctttaatc
     1321 acccgcataa agacgctgtc agattcttct ttcaggctct cttcatagct catggcgaac
     1381 tcttccaggt cacgtcttac agctcgcgtc agctcatcga cctcggttcg cgttaactcc
     1441 cctgttttta ttacgcgctc gcgcgcctgt tccaccagtg cgtcgatatc acgttcgcca
     1501 ttgcgcaggc gttcgctcag tgacgcgacc agttcacggt aatattgagc aaccttgttc
     1561 atcgtttcat ctcccgggtc gttaactgac tataataata gacgttcttt gttccttgct
     1621 ttgctaatac gcccacaaac tctgtaaatt aatgtatgaa taatccgggc atcatttcaa
     1681 cgtcgagtgc gcgaaaggct gttttgacgc ggacgtttgg gctatgctat gcggatctga
     1741 aaaaccacct caacgctaca tttgtagccg tattgaaaac aggaccactg gctgccatgc
     1801 aagagcaata ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc
     1861 gcacatttga agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc
     1921 cctatccttc tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga
     1981 tcgcccgcta ccagcgtatg ctgggcaaaa acgtcctgca gccgatcggc tgggacgcgt
     2041 ttggtctgcc tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt
     2101 acgacaacat cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga
     2161 gccgcgagct ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg
     2221 agctgtataa aaaaggcctg gtatataaga agacttctgc ggtcaactgg tgcccgaacg
     2281 accagaccgt actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca
     2341 aagttgaacg taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc
     2401 tgctcaacga tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta
     2461 actggatcgg tcgttccgaa ggcgtggaga tcaccttcaa cgttaacgac tatgacaaca
     2521 tgctgaccgt ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag
     2581 ctgcgggtca tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta
     2641 ttgacgaatg ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag
     2701 gcgtcgatac tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg
     2761 cagcaaactt cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccgggtcacg
     2821 accagcgcga ctacgagttt gcctctaaat acggcctgaa catcaaacca gttatcctgg
     2881 ctgctgacgg ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt
     2941 tcaactctgg cgaattcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata
     3001 aactgactgc gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg
     3061 tttctcgtca gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg
     3121 taatgccgac cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtgatggacg
     3181 gcattaccag cccgattaaa gcagatccgg agtgggcaaa aactaccgtt aacggtatgc
     3241 cagcgctgcg tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc
     3301 gctacacttg cccagagtac aaagaaggta tgctggattc cgaagcggct aactactggc
     3361 tgccggtgga tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc
     3421 gcttcttcca caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac
     3481 agttgctgtg tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg
     3541 aacgtaactg ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg
     3601 tgaaagcgaa agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca
     3661 agtcgaagaa caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg
     3721 ttcgtctgtt tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg
     3781 gtgtggaagg ggctaaccgc ttcctgaaac gtgtctggaa actggtttac gagcacacag
     3841 caaaaggtga tgttgcggca ctgaacgttg atgcgctgac tgaagatcag aaagcgctgc
     3901 gtcgcgatgt gcataaaacg atcgctaaag tgaccgatga tatcggccgt cgtcagacct
     3961 tcaacaccgc aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccaaccg
     4021 atggcgagca ggatcgcgct ctgatgcagg aagcgctgct ggccgttgtc cgtatgctta
     4081 acccgttcac cccgcacatc tgcttcacgc tgtggcagga actgaaaggc gaaggcgata
     4141 tcgacaacgc gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg
     4201 tcgtggtgca ggttaacggt aaagtccgtg ccaaaatcac cgttctggtg gacgcaacgg
     4261 aagaacaggt tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg
     4321 ttaccgtgcg taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag
     4381 cgcgggagga agcgtgcgat atctggcaac attgttgtta tctctggcgg tgttaatcac
     4441 cgccgggtgt ggctggcatc tgcgtgatac cacgcaggtt ccttccacta tgaaggtcat
     4501 gatcctggac tcaggcgatc cgaacgggcc attaagccgt gcagtgcgta accagttacg
     4561 tctgaatggt gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccctgcg
     4621 tttgggtaaa gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc
     4681 agagtatcag atgatcatga cggtgaatgc gaccgtgttg atccccggcc gtgatatcta
     4741 cccgattagc gccaaagtct tccgttcgtt ctttgataac ccgcaaatgg cgttagcgaa
     4801 agataacgaa caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat
     4861 tcgtaagctg ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac
     4921 cacaacggat actccggcaa cgcctgcacg cgtctccacc acgctgggta actgatgatt
     4981 cggttgtacc cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt
     5041 ttacttggta acgatcctct gttattgcag gaaagccagg acgctgttcg tcaggtagct
     5101 gcggcacaag gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat
     5161 gcgatctttt cgttatgcca ggctatgagt ctgtttgcca gtcgacaaac gctattgctg
     5221 ttgttaccag aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga
     5281 cttctgcatg acgacctgct gttgatcgtc cgcggtaata aattaagcaa agcgcaagaa
     5341 aatgccgcct ggtttactgc tcttgcgaat cgcagcgtgc aggtgacctg tcagacaccg
     5401 gagcaggctc agcttccccg ctgggttact gcgcgcgcaa aacagctcaa cttagaactg
     5461 gatgacgcgg caaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct
     5521 caggcactgg agcgtttatc gctgctctgg ccagacggca aattgacatt accgcgcgtt
     5581 gaacaggcgg tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg
     5641 atgggaaaaa gtaagcgcgc attgcatatt cttcagcaac tgcgtctgga aggcagcgaa
     5701 ccggttattt tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc
     5761 cagtctgccc atacgccact gcgtgcgttg tttgataagc atcgggtatg gcagaaccgc
     5821 cggggcatga tgggcgaggc gttaaatcgc ttaagtcaga cgcagttacg tcaggccgtg
     5881 caactcctga cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca
     5941 gagctgaaag ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac
     6001 ggttgatatg aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg
     6061 tcatctaaaa cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaatcat
     6121 ccctaataat gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca
     6181 catgcttgaa ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa
     6241 gcgcaatgcc ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc
     6301 ggacgtgccg ctggcgttta ttattggtca ggattcactg ctgacctttc cgacctggta
     6361 cgaatacgaa acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc
     6421 acttgaaatg gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc
     6481 ggaagatctt caccttcagc ctgccggtaa aatttatctg gctgaaacgc cgtggtttaa
     6541 catctcggcg accatcatcc gcgaacgttt gcaaaacggt gaatcgtgtg aggatttatt
     6601 gccggaaccg gtactgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt
     6661 acggagggca tgatgcgact gtggttaatt cgtcatggtg aaacgcaagc gaatatcgat
     6721 ggtctttaca gcggtcatgc gcccaccccc ctgaccgcgc gcggtattga gcaagcgcaa
     6781 aatctgcata cgctgctaca tggtgtttcc tttgatctgg ttttatgcag tgaactggaa
     6841 cgggcacagc ataccgcacg actggttctc agtgaccgcc agctccccgt gcaaatcatc
     6901 cctgaactca acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg
     6961 caagaagatg ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc aatccccacg
     7021 aacggtgaag gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt
     7081 gaatttcagc actatcagaa tattttagtc gtcagccatc agggtgtact gagtctgtta
     7141 atcgcccgtt taattggcat gcctgccgag gctatgtggc attttcgcgt tgactagggg
     7201 tgttggagcg ccattgatat caaccaaaaa ttcgcgacgc tacgcgtcct caatagccgt
     7261 gccatcgggg tcgagaatgc atgacttttc tgttttttta cgggtaagcc gcaacgacca
     7321 ttgacagacc cgggcaggct gatattctcc gcagccagac ttttttcgcc agacacgact
     7381 ttgtagaaat tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc
     7441 ccactttcga aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac
     7501 tgacagacca tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact
     7561 ccaggatttt gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga
     7621 cgttcaaggc aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg
     7681 tcatgttatg tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc
     7741 gctcggcgta gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat
     7801 tgtccatgtc atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta
     7861 atgcgtgaag ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg
     7921 ttttaccgag tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc
     7981 ggccggaaaa cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca
     8041 gatgttggcg gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg
     8101 ggatacgccg cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtcag
     8161 tctactgatt ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg cggagcagag
     8221 ctggtcgctg tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag
     8281 tctgtaccgg gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag
     8341 ctttgagtag aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga
     8401 gtccgcgctg tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg
     8461 cgtgcttatc gccaacctgt ataatctgca aattgttcgc tttaccgact accagactcg
     8521 ctctaatgaa aaccgcatta agctggtgcc tatcgcgccc agccgcggca ttatctacga
     8581 ccgtaatggt atccctctgg ccctcaaccg cactatctac cagatagaaa tgatgccaga
     8641 gaaagtcgat aacgtgcagc aaacgctgga cgctttgcgc agcgtggttg atctgaccga
     8701 tgacgatatt gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc
     8761 ggtgaaaact aacctgaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt
     8821 tccgggtgtc gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac
     8881 ccacgtcatc ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga
     8941 cggcaaactg gccaactatg cggcaacgca tgatatcggt aagctgggca ttgagcgtta
     9001 ctatgaagac gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg
     9061 gcgtgttatc cgccagttaa aagaagtacc accgcaagcc ggacacgata tttacctgac
     9121 gctggatctc aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt
     9181 ggttgtcacc gatccgcgta caggtggggt gctggcgctg gtttccacgc ctagttatga
     9241 cccaaacctg tttgttgacg gtatctccag caaagattat tccgccttgt tgaacgcccc
     9301 gaatacaccg ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa
     9361 accctatgtg gcggtttcgg cattgagcgc cggggtgatc acgcgcaata cgacgctgtt
     9421 tgacccaggc tggtggcaac tgccaggttc ggaaaaacgt tatcgtgact ggaaaaaatg
     9481 gggccacggg cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta
     9541 tcaggtggcc tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta
     9601 tggtcattac accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga
     9661 atggaaacag aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat
     9721 cggtcagggt tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat
     9781 taatgacggt atcgtgaagg ttcctcattt gctgatgagc accgccgaag acggcaaaca
     9841 ggtgccatgg gtacagccgc atgaaccgcc cgtcggcgat attcattccg gttactggga
     9901 gctggcgaaa gacggtatgt acggtgttgc taaccgccct aacggtacgg cgcataaata
     9961 ctttgccagc gcaccgtaca aaattgcggc gaaatccggt accgctcagg tcttcggtct
    10021 gaaagcgaac gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact
    10081 gatgaccgcc tttgcgccat acaacaatcc gcaagtggct gtcgccatga ttctggagaa
    10141 cggtggtgcg ggtccggcgg ttggtacact gatgcgccag atcctcgacc acattatgct
    10201 gggtgataac aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca
    10261 ttaatcatga cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc
    10321 acaatgctgc tgatcttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc
    10381 agcggtcagg atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctagtc
    10441 atcatggtgg tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc
    10501 tatatcatct gtattatttt gctggtggcg gtagatgctt tcggtgccat ctctaaaggt
    10561 gctcaacgct ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata
    10621 gccgtaccac tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag
    10681 aacactggca tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct
    10741 gacctgggaa catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc
    10801 cttagctggc gtctgattgg cgtcgcagta gtgctggtgg cggcgttcat tccgattctg
    10861 tggttcttcc tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct agacccggaa
    10921 tcagacccac tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc
    10981 ggattacgcg gcaaaggctg gctgcacggc actcagtcac agcttgaatt tctccccgaa
    11041 cgccataccg actttatctt cgcggtactg gcggaagagc tgggattagt gggcattctg
    11101 attctgctcg ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg
    11161 caaaccacct ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc
    11221 ttcgtaaata ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg
    11281 gtcagttatg gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca
    11341 atccacaccc acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca
    11401 gtggctcggg atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca
    11461 gcaacagacg gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag
    11521 cggggcggac ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga
    11581 cggtaaaagc tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc
    11641 aatctatgat gccgaacccg gcagtaacct gacggcctct ggcgaagctt tcgatccgac
    11701 acagctgacg gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct
    11761 ggctaacggg cgaatgatcg tggtgcgcat taatgatcgc ggcccttacg gcaacgaccg
    11821 cgttatttca ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt
    11881 tcgtatcgat ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg
    11941 taccacagtc gccaaacaga cttacgccct gccagcacct cccgatttaa gcggtggcgc
    12001 gggaacaagt tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct
    12061 aaaaagcgaa gatccgaccg gcgcgccggt aaccagtagc ggtttcctcg gcgctccaac
    12121 gaccttagcg cctggtgtac tggaaggcag cgaaccgacg cctgcgccac agcccgttgt
    12181 tacagctccg tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc
    12241 gcaaagcgcc agcggcaact ttatggtgca agtcggggcc gtaagcgatc aggctcgtgc
    12301 gcaacagtac caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa
    12361 tggcgcggtc tggcggatcc agcttggccc attcgccagc aaagccgaag ccagtacctt
    12421 gcagcaacgt ttgcaaaccg aagcccaatt acagtcattt attactaccg cgcagtagcg
    12481 taaagcaggc atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag
    12541 tcagatgcct gccggtagtg catttgctat agtagggcac ttttttaatt ccatcacgga
    12601 tgtcgtagtt cagaccatga ataccatttt ttccgctcgt atcatgaagc gcctggcgct
    12661 caccacggct ctttgcacag cctttatctc tgctgcacat gccgatgacc tgaatatcaa
    12721 aactatgatc ccgggtgtac cgcagatcga tgcggagtcc tacatcctga ttgactataa
    12781 ctccggcaaa gtgctcgccg aacagaacgc agatgtccgc cgcgatcctg ccagcctgac
    12841 caaaatgatg accagttacg ttatcggcca ggcaatgaaa gccggtaaat ttaaagaaac
    12901 tgatttagtc actatcggca acgacgcatg ggccaccggt aacccggtgt ttaaaggttc
    12961 ttcgctgatg ttcctcaaac cgggcatgca ggttccggtt tctcagctga tccgcggtat
    13021 taacctgcaa tcgggtaacg atgcttgtgt cgccatggcc gattttgccg ctggtagcca
    13081 ggacgctttt gttggcttga tgaacagcta cgttaacgca ctgggcctga aaaataccca
    13141 cttccagacg gtacatggtc tggatgctga tggtcagtac agctccgcgc gagatatggc
    13201 gctgatcggc caggcattga tccgtgacgt accgaatgaa tactcgatct ataaagaaaa
    13261 agaatttacg tttaacggta ttcgccagct gaaccgtaac ggcctgttat gggataacag
    13321 cctgaatgtc gacggcatca aaaccggaca cactgacaaa gcaggttaca accttgttgc
    13381 ttctgcgact gaaggccaga tgcgcttgat ttctgcggtg atgggcggac gtacttttaa
    13441 aggccgtgaa gccgaaagta aaaaactgct aacctggggc ttccgtttct tcgaaaccgt
    13501 taacccactg aaagtaggta aagagttcgc ctctgaaccg gtttggtttg gtgattctga
    13561 tcgcgcttcg ttaggggttg ataaagacgt gtacctgacc attccacgtg gtcgcatgaa
    13621 agatctgaaa gccagctatg tgctgaacag cagtgaattg catgcgccgc tgcaaaagaa
    13681 tcaggtcgtc ggaactatca acttccagct tgatggcaaa acgatcgagc aacgcccgct
    13741 ggttgtgttg caagaaatcc cggaaggtaa cttcttcggc aaaatcattg attacattaa
    13801 attaatgttc catcactggt ttggttaaaa attgaacact tgaaagtgta atttccgtcc
    13861 ccatatacta agcatcagta aaaaactccc gccttctggc gggagttgct atttaattac
    13921 gttacgccgg agctgacatg aaaaccaaac ttaacgaact gcttgaattc cctactcctt
    13981 ttacttacaa agttatgggg caggcgttac ctgagctggt tgatcaggtg gttgaagtgg
    14041 tacagcgcca tgcgccaggt gactacaccc caacggtaaa accaagcagc aaaggcaact
    14101 accactcggt atctatcact atcaacgcca ctcatatcga gcaggttgaa acactgtatg
    14161 aagaactagg caaaatcgat attgtccgca tggttctgta actcgcttct ccgttacccg
    14221 gcccctggtc gggtaactcc cctctcctcg ctgtgatata cttttccccc acttttactc
    14281 attctccacg gagatgcagt tttgtatcag gataaaattc ttgtccgcca gctcggtctt
    14341 cagccttacg agccaatctc ccaggctatg catgaattca ccgatacccg cgatgatagt
    14401 acccttgatg aaatctggct ggtcgagcac tatccggtat tcacccaagg tcaggcagga
    14461 aaagcggagc acattttaat gccgggtgat attccggtga tccagagcga tcgcggtggg
    14521 caggtgactt atcacgggcc ggggcaacag gtgatgtatg tgttgcttaa cctgaaacgc
    14581 cgtaaactcg gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt gaataccctg
    14641 gctgaactgg gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa
    14701 aagaaaattt gctcactggg tttacgtatt cgacgcggtt gttcattcca cggtctggca
    14761 ttaaacgtca atatggatct ttcaccattt ttacgtatta atccttgtgg gtatgccgga
    14821 atggaaatgg ctaaaatatc acaatggaaa cccgaagcga cgactaataa tattgcacca
    14881 cgtttactgg aaaatatttt agcgctacta aacaatcctg acttcgaata tattaccgct
    14941 taattgcata cctcaatggc ccaatttaca ctgggtcatt actcataata cctgctaatg
    15001 ctttacatct ccttcgcgtt tgtatacttt ctttatcact tgcagaaatg cggtgctacg
    15061 ccttgctccg taactcaccc cattgattaa cctattgaaa tagttgatta tggtgagcaa
    15121 aagcgttccg ttccaattag acataagatt attgtaattt ttttaaatat tatttttcca
    15181 tgaataaaat tggagtaagt gcgtggatag taataatcaa attgaaccct gtttaagcag
    15241 aaagtcgtca gaaggtaaac cacaaatatt tacaacctta cgaaatattg atcttaacct
    15301 tctgactatt tttgaagctg tatatgtaca taaagggatc gttaatgcag cgaaagtgct
    15361 taatctgacc ccctcggcaa tcagtcagtc tattcagaaa ctgcgcgtta tattccctga
    15421 cccattgttt attcgcaaag gccagggtgt cactcctacc gcatttgcga tgcatctaca
    15481 tgagtatatc agtcagggcc ttgagtccat tcttggcgcg ctggatatcg aaggaagcta
    15541 tgataagcaa cgaacgataa ctattgcgac cactccctcg gtcggagccc tggtccttcc
    15601 tgtcatctat cgggcgatta aaactcacta tccgcagctt ttactgcgca acccgcccat
    15661 cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt
    15721 ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc gacaatatgg tgttaatttg
    15781 ccgcgaggga aatccactac tctctttaga agatgacaga gagactatcg acaacgctgc
    15841 gcatgtactc ctgttaccgg aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga
    15901 gatgtttccg gaccggcaaa tcaatttcac cagctacaac attttgacaa tcgctgcact
    15961 ggttgccaac agtgacatgt tagcgattat tcccagccgt ttttataacc tgtttagtcg
    16021 ttgctggccg ctggaaaaac tgccttttcc gtccttaaat gaggagcaaa tagatttctc
    16081 tatccactac aacaaattca gcctgcgtga tcccatcctg cacggggtaa tagatgttat
    16141 ccgtaatgca ttttgaaaaa gtattgttac agccagatca cagcgcagaa cgataaaatg
    16201 gcacaatcca acaacaattt tacattttcg cgaccgcttt ggctgctttc aggtccgttt
    16261 caatgatata ctgccagtcg ttaattcaaa aatagttgat aattacaaca atctattgaa
    16321 ttgaaacgct ttccttcgta attcgcaact ggaacacgca cgctatgagt aaacccattg
    16381 tgatggaacg cggtgttaaa taccgcgatg ccgataagat ggcccttatc ccggttaaaa
    16441 acgtggcaac agagcgcgaa gccctgctgc gcaagccgga atggatgaaa atcaagcttc
    16501 cagcggactc tacacgtatc cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt
    16561 ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg cttcaaccac ggcacagcaa
    16621 cgtttatgat cctcggcgct atttgtaccc gccgttgtcc gttctgtgat gttgcccacg
    16681 gtcgcccggt agctcctgat gccaatgaac cagtgaaact ggcgcagacc attgccgata
    16741 tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga tgacctgcgc gatggcggtg
    16801 cccagcactt tgcggattgc attactgcca ttcgggaaaa aagcccgcaa atcaaaattg
    16861 aaactctggt gccggatttc cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa
    16921 cgccaccaga tgtgttcaac cataacctgg aaaacgtacc gcgtatttac cgtcaggtac
    16981 ggcctggtgc agattacaac tggtcgctga agctgctgga acgctttaaa gaagcgcatc
    17041 cggaaatccc gaccaagtct ggtctgatgg tgggactggg tgaaaccaat gaagaaatta
    17101 ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt
    17161 tgcagccaag ccgccatcac ctgccggttc aacgttacgt tagcccggat gagttcgacg
    17221 aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc
    17281 gctcttctta ccacgccgat ttgcaggcga aagggatgga agttaagtaa tcctgtaata
    17341 attacttaca tctgtctgat aaaaaacccg ctttttg
//
LOCUS       DAZOZD010000074        17263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOZD010000074 DAZOZD010000000
VERSION     DAZOZD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.074"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            34..663
                     /locus_tag="ACHUPB_001987"
     CDS             34..663
                     /locus_tag="ACHUPB_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0758806.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUPB_001988"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHUPB_001988"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0758807.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUPB_001989"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHUPB_001989"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0758808.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="ACHUPB_001990"
     CDS             complement(3031..3228)
                     /locus_tag="ACHUPB_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0758809.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUPB_001991"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHUPB_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0758810.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUPB_001992"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHUPB_001992"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0758811.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUPB_001993"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHUPB_001993"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0758812.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUPB_001994"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHUPB_001994"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0758813.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUPB_001995"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHUPB_001995"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0758814.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUPB_001996"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHUPB_001996"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR0758815.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUPB_001997"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="ACHUPB_001997"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0758816.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUPB_001998"
     CDS             complement(11945..13195)
                     /gene="entS"
                     /locus_tag="ACHUPB_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0758817.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUPB_001999"
     CDS             13306..14310
                     /gene="fepD"
                     /locus_tag="ACHUPB_001999"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0758818.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUPB_002000"
     CDS             14307..15299
                     /gene="fepG"
                     /locus_tag="ACHUPB_002000"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0758819.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUPB_002001"
     CDS             15296..16111
                     /gene="fepC"
                     /locus_tag="ACHUPB_002001"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0758820.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPB_002002"
     CDS             complement(16108..17241)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUPB_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0758821.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4043 a         4920 c         4500 g         3800 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata atcccctcgc ccctttgggg agagggttag ggtgagggga aaatgcacct
    11941 ttagctaact gtcagacgct gtcacctgcg gcggcgtctg gcgaaaacgt cgcaactcca
    12001 ccagcaccag caataacaac acgccgataa tcaacaaacc aaaaccgctc gcgcttgcgg
    12061 aagcaaccgg tgtcatcatc gcgcccaaac cacccaacag cgccgcgcct atggcatcgc
    12121 ccgtcacgtt ctgcgccgtc cacaaaccgt taatccgccc taacatcgct tccggggttt
    12181 gcgtttgcag cattgtatat tgcagcaacg aactaaccgc gctcagccag ccgaacagcg
    12241 ccagacaaac cacgcctaaa atccacatcg gcatcaggcc aaacagacca atggcgagga
    12301 acgatcccag cgtggagagc agcatcaata accctggtcg cgcactatgt gccagcttcc
    12361 cgctggttaa cgcaccaata gccgcaccga gcgggatcgc cgcatagaga aaaccaatct
    12421 gtgccgccga catctgccag ttgtcagcca gcgccggata cagtacccgc accgcgctcg
    12481 ccatcgtcaa taaaccaccc agcaacgcaa tccctcctac cagcgggctg gcgagcagaa
    12541 aacgaaatcc tgccagtaat gatttcaacg gatgctcgcg cggctgcggt ggcggtggca
    12601 acgccggaag gcttaacaac ggtagcaagg taataaacgt gcccgccgcc gccagcccgt
    12661 agttccaggc tacgccgccg gtcgccagca ataaaccgcc aatcatgggc gaaatcaccg
    12721 accccagacg cacggtcaac atggtgatcg ccccggcctg cattaagttt tcacgcccta
    12781 ccagtgcagg tgttgccgcc agtagcgccg taacaccaag tgatgcgaaa aaaccatccc
    12841 ataaaccaag taaatagatt gccagcaatg acggctccgg cagcagcgcg ttcaggcaca
    12901 gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa aatcactttt ttgcgctcat
    12961 agcgatccgc cagcacaccg ccaaccatca ggccaacaaa catcgcgccg ccggtcagcg
    13021 tcaccgaaag ccccacctgc caggtagaat gcgtcatcat ctggatctgc accggcaccg
    13081 cgacgccgag caaacccaga gacacaattg agataaaacg agcgaggaat actgcgcgaa
    13141 acgccgggtg cgttttcaac aggctgaggt taagcagcca ggattgttta ttcattacaa
    13201 tgccttgcca tcaaatattt taaataccta ttcttaggct gcacatgcta acatatccaa
    13261 ataagatcga taacgataat taatttcatt atcatggaag ttcgtatgtc tggttctgtt
    13321 gccgtgacac gcgccattgc cgtgcccgga ttgctgttat tactgattat cgcgacggca
    13381 ttaagcctgc tcattggggc aaaatcactc cccgcgtcag tagtgctgga ggccttctca
    13441 ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg cgcgactgcc gcgcaccctt
    13501 gccggtttac ttgcaggcgg tgcgcttggg cttgctgggg cgttaatgca aaccctcacc
    13561 cgaaatccac ttgccgaccc cggcttgctt ggcgtgaacg ccggagccag ctttgccatt
    13621 gtgctgggtg cggcgctgtt tggttactca tccgcgcagg aacaactggc gatggccttc
    13681 gccggggcgc tggtggcctc attgattgtt gcctttatcg gcagtcaggg cggcgggcag
    13741 ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg cggcggtgct ggaagggctg
    13801 accagcggca tcgccctgct taatcctgac gtctacgatc aactgcgttt ctggcaagcc
    13861 ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg tgctgatccc ggttctgatc
    13921 gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca gtttgagcct cggcagtgac
    13981 accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt tgattggtct gctggcgatt
    14041 accgtgcttt gtggtagtgc gacggcggta gttggcccga ttgcctttat cggcctgatg
    14101 atgccgcaca tggcgcgctg gctggtaggt gccgatcatc gctggtcgct gcccgtcacg
    14161 ctacttgcta cccctgccct gctgctgttt gccgatatca tcgggcgggt gattgtgccc
    14221 ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg caccggtgct gatcttcctc
    14281 gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc tcgccgatta ctcatcacct
    14341 gtttgctgct gatttccgcc tgtgtggttg caggtatctg gggattacgc agcggtgccg
    14401 tcacgctgga aatctcgcag gtattcgccg cgttgatggg cgatgcgccg cgcagtatga
    14461 cgatggtggt caccgaatgg cgtttaccac gcgtgctgat ggcgctgttg attggcgcgg
    14521 cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg taacccgctc ggcagccctg
    14581 acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct ggtggcgatg gtgctgtttg
    14641 gtcaggacct gacggctatc gcgctggcag caatggtggg cggcattgtc acttctctgt
    14701 tggtctggct gctcgcctgg cgtaacggta tcgacacctt tcggttgatt attatcggca
    14761 tcggcgttcg cgccatgctg gtggccttta atacttggct gttgctgaaa gcgtctttag
    14821 aaacggcgct aacagcgggt ttgtggaatg ccggatcgct caatggcctg acgtgggcaa
    14881 aaacttcgcc ttccgcaccc atcatcatat tgatgctcat tgccgccgcc ttgctggtgc
    14941 ggcgaatgcg cttgctggag atgggcgatg ataccgcctg cgcgctgggc gtcagcgtcg
    15001 agcgttcgcg tctgttaatg atgctggttg cagtggtgct taccgctgcg gcaacggcgc
    15061 ttgccgggcc gatttccttt attgctttag tcgcgccgca cattgcccga cgcattagcg
    15121 gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg agcgctgtta ctgctggcgg
    15181 ccgatctctg cgcccaacaa ctgtttatgc cgtatcaact tccggttggc gtcgttaccg
    15241 tcagcctcgg cggtatttac cttatcgtct tgttaattca ggagtctcgc aaaaaatgac
    15301 cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg ggatatggca aatataccgt
    15361 tgcggaaaat ctgactgtag aaattcctga tggtcacttc acggcaatta tcggaccaaa
    15421 tggctgtggt aaatccacgt tactgcgtac cttaagccgc ctgatgacgc ctgctcacgg
    15481 gcatgtctgg ctggatggcg agcacattca acattacgcc agtaaagaag ttgcacgccg
    15541 gattggtctg ttggcgcaaa atgctaccac gccgggcgat atcaccgtgc aggagctggt
    15601 ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc tggcgcaaag aggatgaaga
    15661 agcggtaacg aaagcgatgc aggccacgag aataactcat ctggcagatc aaagcgtgga
    15721 taccctttct ggcggacaac gccagcgagc gtggatcgcg atggtgctag cccaggaaac
    15781 ggcaattatg ctgctcgacg aacccacgac ctggctggat atcagtcatc agattgatct
    15841 actggaactg ttaagcgaac tgaaccgcga gaaaggctat accctggcgg cggtgctgca
    15901 cgatcttaat caggcctgtc gttacgccag ccatttgatt gcattgcggg aagggaaaat
    15961 tgttgctcag ggagcgccga aggagattgt cactgctgaa ctgattgagc gcatttatgg
    16021 tctgcgatgc atgatcattg acgatccggt agccggaacg ccgcttgtgg tgccgctcgg
    16081 acgaacggca ccttcaaccg caaatagtta aactaagtgg tctgccatca tggcatcctg
    16141 ttttctggat gccatcgcat agcgcaataa cacgctacca caagccacca tcccgccgat
    16201 cagcgcggaa aggatcacaa taatcgcctt acccggaccg tcttttttca ctggcaatga
    16261 cgggcttaac tgatatttaa acggcgtaaa attcacatcg ttgacatttg cttttgttaa
    16321 ttgctcgaca agatactgcc gattacgtaa ttcaccgttc agttccgcaa cgtcagtaac
    16381 cgctttttca atttccagtt tgcgttcaat accgtctgcg ccgagagaaa tagagaaatc
    16441 ggggtcatct ttaacggcct gaccattact gtatacgggc tttttaattc ctgccgcgtt
    16501 ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca tcaagttgat ttttcgtttt
    16561 aatgcgatcc tgagccagtt tttctttttc aaactgggtt ttgatctcca gtttattacg
    16621 gacgttttct atcgactctt tcaccaccaa cgcagagata taatcgatat acccgctcaa
    16681 aacggtctgc gcctctttac tggttggcgc ggtaaaactt agcgtccagg aggtatacag
    16741 tgacggttca tcttttttct tactggcatt gtcatcaacc gctttcattt tttcgctcaa
    16801 tgcaacaata gcgcgatgca aatccagttc atcgattttc gcctctttta attggtccat
    16861 cacataaggt gatgaacgca ggtactcttc cagcaagcta acagactgaa acttcttgat
    16921 aaacaggtta aatgcaccgc cacggtcgat attaacatcg agatcaagaa cgcggagttt
    16981 ggtaaaggtt ttctccaggt cctgccactg aatggcttcg gcgggagtaa taacggctga
    17041 gctggtccat ttttgcggca ggatgaaaga gatcagtaaa cctgcgcagg caaatgcaaa
    17101 aacgaccgcc atgaccgttt ttttggcccg ccataaaact tcgattagat taagtaaatc
    17161 aatttcatta ttactgggcg acgccagagg atactcagaa aaatgagcgt cacttccctg
    17221 tttaatattc aatgatgaca tggggtataa cctgaaaatg agt
//
LOCUS       DAZOZD010000075        16734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOZD010000075 DAZOZD010000000
VERSION     DAZOZD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.075"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..262)
                     /locus_tag="ACHUPB_002003"
     CDS             complement(<1..262)
                     /locus_tag="ACHUPB_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0758822.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="ACHUPB_002004"
     CDS             369..665
                     /locus_tag="ACHUPB_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0758823.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="ACHUPB_002005"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="ACHUPB_002005"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0758824.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="ACHUPB_002006"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="ACHUPB_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0758825.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="ACHUPB_002007"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="ACHUPB_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0758826.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="ACHUPB_002008"
     CDS             complement(3472..3645)
                     /locus_tag="ACHUPB_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758827.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /gene="dinQ"
                     /locus_tag="ACHUPB_002009"
     CDS             3673..3756
                     /gene="dinQ"
                     /locus_tag="ACHUPB_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0758828.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="ACHUPB_002010"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="ACHUPB_002010"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0758829.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="ACHUPB_002011"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="ACHUPB_002011"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0758830.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="ACHUPB_002012"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="ACHUPB_002012"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0758831.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="ACHUPB_002013"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="ACHUPB_002013"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0758832.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="ACHUPB_002014"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="ACHUPB_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR0758833.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            10765..10818
                     /locus_tag="ACHUPB_002015"
     CDS             10765..10818
                     /locus_tag="ACHUPB_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758834.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="ACHUPB_002016"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="ACHUPB_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0758835.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="ACHUPB_002017"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="ACHUPB_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0758836.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="ACHUPB_002018"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="ACHUPB_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0758837.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="ACHUPB_002019"
     CDS             14052..15254
                     /locus_tag="ACHUPB_002019"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0758838.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="ACHUPB_002020"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="ACHUPB_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
BASE COUNT         4280 a         4257 c         4360 g         3837 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaa
//
LOCUS       DAZOZD010000076        16677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOZD010000076 DAZOZD010000000
VERSION     DAZOZD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.076"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            77..1216
                     /gene="arnB"
                     /locus_tag="ACHUPB_002021"
     CDS             77..1216
                     /gene="arnB"
                     /locus_tag="ACHUPB_002021"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0758839.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1220..2188
                     /gene="arnC"
                     /locus_tag="ACHUPB_002022"
     CDS             1220..2188
                     /gene="arnC"
                     /locus_tag="ACHUPB_002022"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0758840.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2188..4170
                     /gene="arnA"
                     /locus_tag="ACHUPB_002023"
     CDS             2188..4170
                     /gene="arnA"
                     /locus_tag="ACHUPB_002023"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR0758841.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4167..5057
                     /gene="arnD"
                     /locus_tag="ACHUPB_002024"
     CDS             4167..5057
                     /gene="arnD"
                     /locus_tag="ACHUPB_002024"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0758842.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5057..6709
                     /gene="arnT"
                     /locus_tag="ACHUPB_002025"
     CDS             5057..6709
                     /gene="arnT"
                     /locus_tag="ACHUPB_002025"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0758843.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6706..7041
                     /gene="arnE"
                     /locus_tag="ACHUPB_002026"
     CDS             6706..7041
                     /gene="arnE"
                     /locus_tag="ACHUPB_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0758844.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7041..7427
                     /gene="arnF"
                     /locus_tag="ACHUPB_002027"
     CDS             7041..7427
                     /gene="arnF"
                     /locus_tag="ACHUPB_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0758845.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7421..7687)
                     /gene="pmrD"
                     /locus_tag="ACHUPB_002028"
     CDS             complement(7421..7687)
                     /gene="pmrD"
                     /locus_tag="ACHUPB_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0758846.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7731..9152)
                     /gene="menE"
                     /locus_tag="ACHUPB_002029"
     CDS             complement(7731..9152)
                     /gene="menE"
                     /locus_tag="ACHUPB_002029"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0758847.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(9149..10111)
                     /gene="menC"
                     /locus_tag="ACHUPB_002030"
     CDS             complement(9149..10111)
                     /gene="menC"
                     /locus_tag="ACHUPB_002030"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0758848.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAEMADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10111..10968)
                     /gene="menB"
                     /locus_tag="ACHUPB_002031"
     CDS             complement(10111..10968)
                     /gene="menB"
                     /locus_tag="ACHUPB_002031"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0758849.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10983..11741)
                     /gene="menH"
                     /locus_tag="ACHUPB_002032"
     CDS             complement(10983..11741)
                     /gene="menH"
                     /locus_tag="ACHUPB_002032"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0758850.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11738..13408)
                     /gene="menD"
                     /locus_tag="ACHUPB_002033"
     CDS             complement(11738..13408)
                     /gene="menD"
                     /locus_tag="ACHUPB_002033"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0758851.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13497..14792)
                     /gene="menF"
                     /locus_tag="ACHUPB_002034"
     CDS             complement(13497..14792)
                     /gene="menF"
                     /locus_tag="ACHUPB_002034"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0758852.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14871..15176)
                     /gene="elaB"
                     /locus_tag="ACHUPB_002035"
     CDS             complement(14871..15176)
                     /gene="elaB"
                     /locus_tag="ACHUPB_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0758853.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15231..15674)
                     /locus_tag="ACHUPB_002036"
     CDS             complement(15231..15674)
                     /locus_tag="ACHUPB_002036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0758854.1"
                     /translation="MHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDNRH
                     ILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDTCTHHWP
                     DKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15757..16674
                     /gene="rbn"
                     /locus_tag="ACHUPB_002037"
     CDS             15757..16674
                     /gene="rbn"
                     /locus_tag="ACHUPB_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0758855.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3849 a         4543 c         4284 g         4001 t
ORIGIN      
        1 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
       61 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
      121 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
      181 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
      241 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
      301 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgttgggtgc
      361 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
      421 gtcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
      481 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
      541 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
      601 tttttcattt catgccatta aaaatattac ctgtgcggaa ggtggcctga ttgtaactga
      661 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
      721 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
      781 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagcaa aattagagca
      841 cctcaacacc catcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
      901 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
      961 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
     1021 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
     1081 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcttt
     1141 gccgctgttc ccggatatga ctaccgccga tgccgaccgt gtcatcacag cccttcagca
     1201 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
     1261 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
     1321 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
     1381 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgct
     1441 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
     1501 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
     1561 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
     1621 ctggtttcgt aaaacagctt cgaagatgat taacaggctt attcagcgca ccaccggcaa
     1681 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
     1741 gttgcactgc catgaacgta gcacctttat cccgattctg gcgaatatct tcgcccgccg
     1801 tgccattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
     1861 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
     1921 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
     1981 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
     2041 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattgc tcggtgaata
     2101 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
     2161 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
     2221 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
     2281 cataccgata atcccgctga aaaagccttt tatggttcgg tggctcgtct ggcggcggaa
     2341 agaggcattc cagtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
     2401 gcccaactct caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
     2461 attttgcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
     2521 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
     2581 ttgcaccgaa tggtaaaacg tgccgatgct ggggccattg tggcgcaact gcgcgttgcc
     2641 attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc cgcgcgccag
     2701 ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat tgcccagcgc
     2761 gaaaacgaag ccacctgttt tggtcacaga acgccagatg acagtttcct tgaatggcat
     2821 aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg gccgggtgcc
     2881 ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca tcctcatgcc
     2941 agcaaagcac agccggggag cgtgatttct gttgcgccac tgctgattgc ctgtggcgat
     3001 ggcgcgctgg aaatcgtcac cggacaggcg ggcgacggca ttactatgca gggctcgcaa
     3061 ttagcgcaga tgctgggcct ggtgcaaggt tcacgcttga atagccagcc tgcctgcacc
     3121 gcccgacgcc gtacccgggt actcatcctc ggggtgaatg gctttattgg caaccatctg
     3181 acagaacgcc tgctgcgcga agatcattat gaagtttacg gtctggatat tggcagcgat
     3241 gcgataagcc gttttctgaa tcatccgcat tttcactttg tcgaaggcga tatcagtatt
     3301 cattccgaat ggattgagta tcacgtcaaa aaatgtgatg tcgtcttgcc gctggtggcg
     3361 atagccacgc cgattgaata tacccgcaac ccgctgcgcg tatttgaact cgattttgaa
     3421 gagaatctgc gcattatccg ctactgcgtt aagtaccgta agcgaatcat cttcccgtca
     3481 acttcagaag tttatgggat gtgtagcgat aaatacttcg atgaggacca ttctaattta
     3541 atcgtcggcc cggtgaataa accacgctgg atttattcgg tgtcaaaaca attacttgat
     3601 cgggtgatct gggcctatgg cgaaaaagag ggtttacagt tcaccctctt ccgcccgttt
     3661 aactggatgg gaccacgact ggataacctt aatgcagcgc gaattggcag ctcccgcgct
     3721 attacgcaac tcattctcaa tctggtagaa ggttcaccaa ttaagctaat tgatggcgga
     3781 aaacaaaaac gctgctttac tgatatccgg gacggtatcg aggcgttgta ccgcattatc
     3841 gaaaatgcgg gaaatcgctg cgacggcgaa attatcaaca ttggcaatcc tgagaacgaa
     3901 gcgagcattg aggaactggg cgagatgctg ctggcgagct tcgaaaaaca tccgctgcgc
     3961 catcacttcc cgccgtttgc gggctttcgt gttgtcgaaa gtagcagcta ctacggcaaa
     4021 ggatatcagg acgtagagca tcgtaaaccg agcatccgca atgcccgccg ctgcctggac
     4081 tgggaaccga aaattgatat gcaggaaacc atcgacgaaa cgctggattt cttcctgcgc
     4141 accgttgatc ttacggataa accatcatga ccaaagtcgg cttacgcatt gatgtcgata
     4201 cctttcgtgg cacccgtgaa ggcgtgccac gtctgctgga aatcttgagt aagcataata
     4261 ttcaggccag catttttttc agcgtcggcc cggacaatat gggccgccat ctctggcgac
     4321 tggtgaagcc acagtttttg tggaagatgc tgcgctcaaa cgcggcatcg ctttatggct
     4381 gggatatttt actggcaggt acggcctggc caggtaaaga gattggtcat gccaatgccg
     4441 atatcattcg tgaagcggct aaacatcacg aagtcggcct gcacgcctgg gatcaccatg
     4501 cctggcaagc ccgtagcggt aactgggatc ggcaaacaat gatcgacgat attgcacgcg
     4561 gtcttcgcac tctggaagag attatcggtc aaccggtaac ctgttctgcc gctgcgggct
     4621 ggcgtgccga ccagaaggtg atcgaagcaa aagaagcgtt ccatttgcgc tacaacagcg
     4681 attgtcgtgg ggccatgccg ttccgtccac tgctcgaatc aggaaaccct ggcactgcgc
     4741 aaattccggt gaccttaccc acctgggatg aagtgattgg tcgggatgtg aaagcagaag
     4801 attttaacgg ttggttactc aaccgcatcc tgcgagataa aggcacgccg gtttatacca
     4861 ttcatgcaga agttgaaggc tgcgcttatc agcataattt tgtggatctc ctcaaacgcg
     4921 cagctcagga aggcgtgaca ttttgccctt taagcgaact gttatcagag acgttgccgc
     4981 tcggacaagt tgttcgcgga aatattgccg gacgtgaagg ctggctgggt tgccaacaaa
     5041 ttgcgggtag tcgctgatga aatcggtacg ttaccttatc ggcctcttcg cgtttattgc
     5101 ctgctattac ctgttaccga tcagcacgcg tctgctctgg caacccgatg aaacgcgtta
     5161 tgcggaaatc agtcgagaaa tgctggcatc cggcgactgg attgtgcccc atctgttagg
     5221 gctacgttat ttcgaaaaac ccattgccgg atactggatt aacagcattg ggcaatggct
     5281 atttggcgcg aataactttg gtgtgcgggc aggcgttatc tttgcgaccc tgttaactgc
     5341 cgcgctggtg acctggttta ctctgcgctt atggcgcgat aaacgtctgg ctctactcgc
     5401 cacagtaatt tatctctcat tgtttattgt ctatgccatc ggcacttatg ccgtgctcga
     5461 tccgtttatt gcattctggc tggtggcggg aatgtgcagc ttctggctgg caatgcaggc
     5521 acagacgtgg aaaggcaaaa gcgcaggatt tttactgctg ggaatcacct gcggcatggg
     5581 ggtgatgacc aaaggttttc tcgcccttgc cgtgccggta ttaagcgtgc tgccatgggt
     5641 agcgacgcaa aaacgctgga aagatctctt tatttacggc tggctggcgg ttatcagttg
     5701 cgtactgacg gttctcccct ggggactggc gatagcgcag cgggagcctg acttctggca
     5761 ctattttttc tgggttgagc atattcaacg ctttgcactg gatgatgccc aacatagagc
     5821 tccgttctgg tactacgtgc cggtcatcat tgccggtagc ctgccgtggc tgggattact
     5881 ccccggtgca ctgtacacag gctggaaaaa ccgcaagcat tccgcaaccg tctatttgtt
     5941 gagctggacg ataatgccgc tgctgttttt ctccgtcgct aaaggtaaat tgcccaccta
     6001 tattctttcc tgctttgcat ctctggcaat gctgatggcg cattacgctt tgctggcagc
     6061 aaaaaataat cctctggcgc tgcggattaa tggctggatt aacatcgctt ttggcttcac
     6121 tggcattatt gccacatttg tggtctcccc gtggggacca atgaacacgc cggtgtggca
     6181 aaccttcgag agctataaag tcttttgtgc ctggtcgatt ttttcgctat gggcattttt
     6241 cggctggtac accttaacaa acgtcgaaaa gacctggtct tttgccgcgc tttgcccgct
     6301 ggggctggcg ttgctggtag gattttcaat tcctgacaga gtcatggaag gaaaacatcc
     6361 gcaatttttt gtcgagatga cacaagaatc acttcagcca agccgctata ttcttactga
     6421 tagcgtcggt gttgccgcag gtctggcatg gagcctgcaa cgcgatgaca tcatcatgta
     6481 tcgccagaca ggtgagttga aatacggcct taattatccg gatgcgaaag ggagatttgt
     6541 cagcggtgat gagttcgcaa actggcttaa tcaacatcgt caggagggga ttattacact
     6601 cgtgctttcg gttgaccgcg atgaagatat caacagtctc gccattccgc ccgcagatgc
     6661 catcgatcgt caggagcgtc tggtgctgat tcagtatcgt cccaaatgat ctggctaaca
     6721 ttagtctttg ccagcttgct tagcgttgcc gggcagttgt gtcagaaaca ggcaacctgc
     6781 tttgtggcga taaacaaacg gcgcaaacat atcgtgctgt ggctgggact ggcgctggct
     6841 tgtcttggtc ttgccatggt gctctggctg ctggtcttgc agaacgtacc ggtaggcatt
     6901 gcctacccga tgttaagtct gaattttgtc tgggtgacgc tggctgcagt aaaactgtgg
     6961 cacgaaccgg tatcgccgcg tcactggtgt ggggtggcgt tcattattgg cggcattgtg
     7021 atcctcggga gtacggtgta atgggcctga tgtggggatt attcagcgtc attattgcct
     7081 cagttgcgca attaagcctg ggttttgcgg cgagtcatct gccgccgatg acgcacttat
     7141 gggattttat cgcggctctg ttagcttttg gcctggatgc ccggatactg ttactcgggc
     7201 tgctgggtta tctgctttcc gttttttgtt ggtataaaac gctacataaa cttgcgctaa
     7261 gcaaagccta tgccttgctc agtatgagtt atgtgctggt atggattgct tcaatggtac
     7321 ttcccggctg ggaagggact ttttcgctga aagcactact gggagtagcc tgtattatga
     7381 gcgggttgat gctgattttc ctgcccacga caaaacaacg ttactgagtt ttccctgcca
     7441 ctttacactg ccgttcccac tcatcggggc tataactgct tgccgagagg actttcaccg
     7501 tatgcagctt ttcacgatta atacaataaa gcgcattctg caaaggcgag agtaaatccc
     7561 ccactttcac ggcgaagtca ctcttcactt cggcgatcat ttttatcgcg ccgccagcat
     7621 cgcagagcat aagtacatgt ctgttatctt gtttattgca gcacgatttt ttgaccagcc
     7681 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
     7741 aacctattat caacggcaat ggaatggcaa aattgcgaag tgaaactaat gatttcgtaa
     7801 tcctggcgtt gcacccactc ttttaacgcc tgacgtgaaa ttttaatacc gccgtttttc
     7861 agctccggcg gtaaagttag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
     7921 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggtcgatga
     7981 ccaaactcct tgtcggcaac ggggacgata aacacctgca aaaccgcagg atgtgcagca
     8041 attacgcgct cgacttcttc cggctgaata ccctctccgc cactgaagaa cagattgtct
     8101 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
     8161 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
     8221 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
     8281 gaaccaacgt ctgccaggcc gtcggcttct ttcgcacaca ccgtggaggc aaactcggtc
     8341 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
     8401 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
     8461 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
     8521 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
     8581 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
     8641 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
     8701 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
     8761 cacgtagcgg catgtgcgcc ttcaacccgc tgaatgtgca gcgacgttaa tgccggaaac
     8821 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
     8881 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
     8941 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
     9001 tcaaccacac cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
     9061 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
     9121 cagtgacgcc acggccagtc agagaagatc ataacaactg ctccagtaca tcagcgtcca
     9181 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
     9241 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
     9301 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
     9361 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
     9421 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
     9481 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
     9541 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
     9601 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
     9661 ccagcaacag attaaccacc atgccgtcgc gcacggcttc gtacaatccc actttgacct
     9721 tcgccacttt ctcgcctggc atatcagcaa gtttgaggat cagatcgtca ggatcgccat
     9781 tacacagtgg tgccgcacgg taattggccg cctgcgggag cgtatctgcc atctccgcca
     9841 atgcacagct tacgccaaag gcaacggaag gcatctgcgg tagctcgcca tcgcctgcca
     9901 gccagttatt tacccaagca agcagcacaa tttgcgcctc ttcccaggtt tcctgactga
     9961 agcccggcag tggggagatc tccccccacc cttcgcgctc gccttcacgc aggcaaacat
    10021 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac catccccgcg tccatgggga
    10081 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    10141 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    10201 cagcatggtg gcgttgcccg ccagctcctg cagacccgcc tgaccgtcac agtcggcgtt
    10261 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    10321 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    10381 cagcgcctgt tttgcatcgt actgacggca caggaaccag atttcacgcg cttttttctg
    10441 tccgacgatg cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    10501 gccagtctga ccgaagatgg cattatctgc cgcgatagtc aggtcgcaca tcatgtgcag
    10561 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    10621 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    10681 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    10741 gccagtcaga atgatcacgc cgacgttgtc gtcataacgc gcatctgcca gtgcctggat
    10801 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    10861 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcaaaacctt cggagcagtc
    10921 gtgccattca accggtgcgt aaagcattgc ttcatcagga taaatcatat tgtgtccttt
    10981 agtcagaaac gcaagatctg cgccagactt gcgataacgc cagcgggatt ttcccgatgc
    11041 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccaaggcg
    11101 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    11161 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    11221 gcgccattat tgttactgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    11281 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    11341 cgctgcgccc attggcgatc ggaacgctga cgttccgcgc gttgttcagc attttgcaac
    11401 ccaggatgcc cgccttcgac aacaacccca caaagccccg gcatcccctg gcaagccgcc
    11461 atcatcgcca cccgtccccc aagcgagtac cccaccaacc agaagttaag gatgttgtaa
    11521 ctaaccaggg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    11581 gccgaaccac cgtgacctgg gagataaaca tacaaccgtg agtagtcggc aaacgcctcg
    11641 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    11701 aaacctggtt tgccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    11761 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca attaccgtgg
    11821 tggttggcgt gcgccaggca tcggcaagtg ccgtttcaag ttcctgccag ttttgcggac
    11881 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    11941 gatagaaacg ctcgcgctcg cttttcggcg ttggcagcag cgagaaaatt tgcccgccgt
    12001 tgttgttcac cacaattaat atcagcggcg cggaaacctg acgcaataac gccagcgcgt
    12061 tgagatcgta aagtgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    12121 gctgaacgcc ggcggcagtc gagagcagcc cgtcgatacc gctggcccca cggttgctgt
    12181 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    12241 taccgacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    12301 cttcgccaaa cgcatcgcga cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    12361 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    12421 tattggcaat taagcggcgt ccacgatggt gcgccggatc aagccgccct tcgatgtcat
    12481 ccacaatcca gtactcttcg ggttcacagc ttgcctgcca ttgcaggagc cgtttgcccg
    12541 tcaggctgct tcccagttgc accacaattt gcgcctgttg cagttcgctg gtcgctttgg
    12601 cattgcctaa ccagagatcg gcacacggca gcggctgtcc ggtttgcgac agcacgtcgc
    12661 caatcagcgg ccagccaaga gtttgcgccc acagtgcaac ttttttgccc tcttccgcac
    12721 tcatgcgccc ggcaaccacc acgccgcgtt tttgtcgcca gaagaaccag tcgcgctgtt
    12781 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcgc
    12841 ccagccgctg ttgccagcta agcccggtgt cgtccatttc gccatacagc ggttcggcaa
    12901 acgggcagtt gatatggact ccaccagaat gaagcgtacc gagagcatgg tcgatggtag
    12961 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    13021 ggtgagaggc gaacattccc ggctgacgaa tcgcctgatt cgcgccgcag tcaatcagct
    13081 ccggcgggcg atcggcggtt aacaggatca gtttttctcc ggttaaccca gcttcaatca
    13141 gtgccggata gagatttgct accgccgtac cggaggtcac aatcaccgcc accggctgct
    13201 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    13261 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagccag
    13321 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    13381 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    13441 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    13501 tccatctgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    13561 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    13621 atttttgctg agcgcaggga aacgcagaat tcgctttgct gtaatgagag atagcccgca
    13681 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    13741 tcgcgcggta agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatta
    13801 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    13861 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaaccg ttggcagata
    13921 tcttccacca ccagcatatt ctcgcgctgg tttttatcat ccgccatcag ccactcacct
    13981 aactgctgcg cctgcttatc atcaggatga tttgctaccg ttcccgccag cgcttcagta
    14041 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    14101 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    14161 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    14221 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    14281 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    14341 ttgatactca ccagtgtggc gataaattct cttgccttaa tcgcatcgtg ctgaagggag
    14401 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    14461 cgtggtaaaa gtaaattgcc ctgcgacggg tcaaacgcat tcagccccca aatgcgtaag
    14521 tcggcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    14581 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    14641 gggtatgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    14701 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    14761 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    14821 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    14881 gccagcaaca gtcctagtac cagcccaacg gccgcgccca caccaattcc ttgccagggt
    14941 ttttcgtgga cgtagtcatc agcacgataa actgcctgct tcgcccgata gtaataactg
    15001 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc acgcgctttc
    15061 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    15121 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    15181 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gggttgttga ttacgcctga
    15241 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    15301 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    15361 ggcttatcag gccagtgatg cgtgcaggtg tcgagggttt tactcatcag ttgctgcccc
    15421 actttttcgc cacgcaacgc ttcactgaca atcacccgac caataacgac cggctcaaga
    15481 tcatcatcac ttttcagaat cctcgcatat gccaccagtt catcattttt ccaaccgaga
    15541 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    15601 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    15661 tcagagtgat gcagatctta ccattcaatc ataatgctct ccttttagat gtatgcctta
    15721 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    15781 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    15841 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    15901 cataccgcct ttaaccctgg gaaactggac aagattttta tcagtcacct tcatggcgat
    15961 catctctttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    16021 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    16081 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    16141 gatggcctgc gcaaagtgac cgcttatccg atggaacacc cactggaatg ttatggctat
    16201 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    16261 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    16321 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    16381 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    16441 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    16501 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    16561 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    16621 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactc
//
LOCUS       DAZOZD010000077        16496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOZD010000077 DAZOZD010000000
VERSION     DAZOZD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.077"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(65..217)
                     /locus_tag="ACHUPB_002038"
     CDS             complement(65..217)
                     /locus_tag="ACHUPB_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0758856.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(482..1216)
                     /gene="cysH"
                     /locus_tag="ACHUPB_002039"
     CDS             complement(482..1216)
                     /gene="cysH"
                     /locus_tag="ACHUPB_002039"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0758857.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1291..3003)
                     /gene="cysI"
                     /locus_tag="ACHUPB_002040"
     CDS             complement(1291..3003)
                     /gene="cysI"
                     /locus_tag="ACHUPB_002040"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0758858.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3003..4802)
                     /gene="cysJ"
                     /locus_tag="ACHUPB_002041"
     CDS             complement(3003..4802)
                     /gene="cysJ"
                     /locus_tag="ACHUPB_002041"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0758859.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5121..5483
                     /gene="queD"
                     /locus_tag="ACHUPB_002042"
     CDS             5121..5483
                     /gene="queD"
                     /locus_tag="ACHUPB_002042"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0758860.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5561..6832
                     /locus_tag="ACHUPB_002043"
     CDS             5561..6832
                     /locus_tag="ACHUPB_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0758861.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6823..7083
                     /locus_tag="ACHUPB_002044"
     CDS             6823..7083
                     /locus_tag="ACHUPB_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0758862.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7100..7675
                     /locus_tag="ACHUPB_002045"
     CDS             7100..7675
                     /locus_tag="ACHUPB_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR0758863.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7823..8683)
                     /locus_tag="ACHUPB_002046"
     CDS             complement(7823..8683)
                     /locus_tag="ACHUPB_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0758864.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8680..9459)
                     /locus_tag="ACHUPB_002047"
     CDS             complement(8680..9459)
                     /locus_tag="ACHUPB_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0758865.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9437..10846)
                     /locus_tag="ACHUPB_002048"
     CDS             complement(9437..10846)
                     /locus_tag="ACHUPB_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0758866.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10868..12322)
                     /locus_tag="ACHUPB_002049"
     CDS             complement(10868..12322)
                     /locus_tag="ACHUPB_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0758867.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12392..13208)
                     /locus_tag="ACHUPB_002050"
                     /pseudo
     CDS             complement(12392..13208)
                     /locus_tag="ACHUPB_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13526..14803
                     /locus_tag="ACHUPB_002051"
     CDS             13526..14803
                     /locus_tag="ACHUPB_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR0758868.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14830..16315
                     /locus_tag="ACHUPB_002052"
                     /pseudo
     CDS             14830..16315
                     /locus_tag="ACHUPB_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3907 a         4352 c         4162 g         4075 t
ORIGIN      
        1 aaaaagggtg caggagacca aaccatcaca attcagtacg cggggtttta cccccgcgtg
       61 gtggctactt cttcgattcg taagcgagaa ctgccttaaa ctccatacca cgttctctga
      121 tactcaactc acacaacgag tcgcctacca tcagcgtgaa tcccagtact gtaaaacaca
      181 gcactatgat tgccacaagg gcatattttg tcagcatgtc ttgcctccct gcaaagaaga
      241 gactaacatt cgaattgttt gggttcgaac gctggcctca ggttgataga aatatcgcct
      301 ggggcttttg tccatctgga acctcgcgaa tgcttaacgc cagacagcct caagcacccg
      361 acgccattct atacctgata attcttccag cggcgttcac gtttaaaatc aggaatactg
      421 ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc gtttaattta tccggcaata
      481 tttacccttc gtgcaatcca cattcccttt ttaagccaaa gaaacgtgtt tcttcttccg
      541 acatgccagg ttcccattta cgggttgtat gggtatcacc gaccgataaa tatccttcat
      601 cccataatgg gtgatatttc aggccatgtt tttgcaggta ctgataaata gttcggttat
      661 cccagtcgat aatcggcagc actttaaata cgccacgctg aattgccagc accggtaaat
      721 tggcgcgact gccggattgt tcacggcgca ggccagcaaa ccaggtttgc gcgttaagtt
      781 ctttcagagc ccggttcatc ggttcgactt tgttgatgtc attgtacttt tcaatgcctt
      841 caacgccctg ctcccacagt tttccgtagc gtgcttcctg ccaggctgcg ctttcggtag
      901 cacggtacac tttcaggttg agcttgagtt tgtccgttaa ctcgtcaata aagcggtagg
      961 tttccgggaa caagtaaccc gtatcggtga ggatcaccgg aatatccggg cgaatttgat
     1021 tcaccagatg caggctcacc gccgcctgaa tgccaaagct ggaagaaagt acatattcac
     1081 cgggcagatt atccagcgcc caggctacgc ggccttcagc gtccagtttt tccagttcgg
     1141 cgttagtttc cgccagcgcc agaatacgat ctaccttcgg cagttcgttc agggcgttta
     1201 gatcgagttt ggacatagat tcctcactgt ttgccttgcc tgatgcgacg ctcacgcgtt
     1261 cttatcaggc ctacaagacc gggctgatgg ttaatcccac aaatcgcgcg ccggatcgag
     1321 caccgggcga atgatgcccg cacgcaccgt aaagtcgccg aagccttcac ccgcttcgcg
     1381 ctctttcgcc cagcgcccta tcagttcatc aagcgacgcc aggatttccg gctcggtgat
     1441 gttttcttta tacatccgtg ggatacgtgt cccaatgcgg ttgccgccaa gatgcaggtt
     1501 gtagcgaccc ggcgctttac ccaccaggcc cacttccgcc agcatcgcgc gaccacaacc
     1561 gttcgggcag cctgttacac gcatcacgat atgctcatcg ctgacaccat gtttcgccat
     1621 taaattatcg atgttgtcga taaaagacgg caggaaacgc tctgcttccg ccatcgccag
     1681 cgggcaagtc gggaatgaca cgcaggccat cgagttttca cgctgcggcg tgacggcatt
     1741 cattaacccg ctctctttgg cgatcttctc gatcttcgct ttctcgcttt ccggtacacc
     1801 ggtgatgatc agattctggt tcgccgtaat gcggaaatcg cctttgtgga tcttcgcgat
     1861 ctccagcagg ccggttttca gcggacgccc cggataatca aggatgcgac cattttcgat
     1921 aaacagcgtc aggtgccagt tatcatcaat gcccttaacc cagccaatac gatcgcctcg
     1981 tccggtgaac tcatatggac ggatcggttc aaatttgatc cccgcgcgac gctccacttc
     2041 cgctttaaac gtctcaaccc ccacgcgctc cagcgtgtat ttggttttgg catttttacg
     2101 atcggttcgg ttaccccagt cacgctgagt tgtcacgacg gcttcggcca ccgccagcgt
     2161 atgctccagc ggcagatagc caaactcgct cgccgtgcgg gcgtaggttt tcttgttgcc
     2221 gtgttcgata gaaagcccac cgcccaccag caggttgaag cctaccagct tgccgttttc
     2281 ggcgattgcc acgaagttca tgtcgttggc atgcagatcg atatcgttct gcggcgggat
     2341 cactaccgtg gttttgaact tacgcggcag gtaggtctga ccaaggatcg gttcttcatc
     2401 agtagtggcg actttttcct ggtcgagcca gatctccgca tacgcgcggg tacgaggcaa
     2461 cagatgctca gaaatcttct tcgcccactc gtacgcttcc gcgtgcagct gcgactcgta
     2521 agggttcgag gtgcagagta cgttacggtt catgtcgtta gctgtcgcca gcgcatcaag
     2581 accgaccgag tgcagcatct ggtgcaccgg tttgacgttc tttttcagaa tgccgtggaa
     2641 ctgaaacgtc tggcggttgg tcaggcgaat gctgccatag atggtgtttt caccggcaaa
     2701 tttgtcgatc gcctgccact gtttagtggt aatcacccca cccggcagac gacagcgaag
     2761 cagcatcgcg tggcgcggct ccagcttctg ttcagcacgt tcggcacgga tgtcgcggtc
     2821 atcctgctga tacatgccgt ggaagcggat cagcaggaag ttgtcgccct taaagccgcc
     2881 ggtcagaccg tcgtttaaat cttccgcaat ggtgccgcgc aggtagttgc tttcaagctt
     2941 catgcgctcg gcgtctgtca gttttccttc gaccactaaa ggccctggat gtttttcgct
     3001 cattagtaga catctcgctg ataacggcgc tctacgcgca gctcacttaa aaattcatcc
     3061 gccgcttcgg tgtccatgcc accaaattcg gcaatcactt ccagaagtgc ctgctcaacg
     3121 tctttcgcca tgcgattagc gtcgccgcag acataaatgt gggcaccgtc attgatccag
     3181 cgccacagct ccgcgccctg ttcgcgcagt ttgtcttgta cataaacttt ttctttttgg
     3241 tcgcgcgacc aggcaagatc gatacgcgtc agcacgccct ctttgacgta gcgctgccac
     3301 tccacctggt agaggaaatc ttccgtaaag tgcgggttgc caaagaacag ccagttttta
     3361 cccggcgctt cgtcggcggc gcgttgctgc ataaaggcac ggaacggcgc gatgccggta
     3421 ccggggccaa tcataatcac tggcgtttgc gggttagcgg gcaggcggaa attgtcgtta
     3481 tgttcgataa atacgcggac ttcgccctct tcttccacgc gatccgcgag gaagctggag
     3541 gcaccaccgg cacgggcgcg gccttccacg tcgtaacgca ccacaccaac ggtgacgtgt
     3601 acttcgttct cgacttccgc ctgcgaggag gcgatggaat acaggcgcgg ggtcagcggg
     3661 cgcagcaggt taatcagcgc ttcggcatcc agttgcgccg gagagaaacg caccatgtcg
     3721 acaatcggcg tggtcgcggc gtaatgctgt aacttcgctt tatcgcccac cagcggcagc
     3781 agcgtttcgc tgcgggtaag cgtggcatag ttctcaacaa tgttggcggt gttgacggtc
     3841 agttcgaagt gccactgtag cgcttcgttc agaggcaacg ttttgccctc gacggtgaca
     3901 ggttcatcgc ctttcagcca cagcagttcg acaagttctt tcaccagtgc cgggtcgttc
     3961 tgataccaga cacccagcgc gtcacccggc tggtaacgca ggcctgaatc gcctaagtca
     4021 atttcgatat gacgaacgtc tttttcagag ttacgcccgg taattttctg gttaaccgaa
     4081 aggctcgcca ccagcggcgc gtctttgctg tacgggctgg tgtggatttc atttaccgcg
     4141 ccagtagcga cggattgcga aggtgccgcg acaggcgcac gcgatttaag cgcatcaacc
     4201 acgcgggcgc gccactcgct ggcagcagcc tggtattcaa catcggcatc gacacggtcg
     4261 agcaggcgtt caccacccag ttccgccagc ttgctgtcga aatctttccc ggactggcag
     4321 aaaaattcat aagagctatc gccgaggcta aacacggcaa acgcggtgtt ttccagcttt
     4381 ggcgcttttt tggagaacag gaacttatgc agcgcgacgg cttcttccgg cggttcccct
     4441 tccccttgcg ttgacgtcac tacgatgagc agtttttcgc tggcgatttg tttgaattta
     4501 tagtcgcccg cgttcaccag cttaacgttc agttttgctg ctaataaatc atcgcgtaat
     4561 gcttcagcaa cccggcgcgc attgccggtt tgcgaggcgg agataatagt tatacccggc
     4621 atttctgcgg ctggcgctgg cgtcgctgca agcgcagcag gctgctgatt gagtacgccc
     4681 cagaaatagc cagaaaccca ggcaagctgg gtaggcgtta aatcggtcgt ggccgcctga
     4741 aggcgtgcca gttgctccgg gttcaacgga agcaacgcgg aaggtgggac ctgtgtcgtc
     4801 atgcgtcgtt atgttccagt aagcaaagct gtttctgcgc cctgtcagcg cccataaaac
     4861 agaagagaag ataaggttaa cggggtgaac ggtgtggatt aaagacggga tagcgataac
     4921 taataaccaa atcgactaac ctgttttagc aatagtcttt aacaacaaaa tagattaacc
     4981 aacccaatga aaaacaaatg aatttagcca atcattaaga taaatcagcg attttgcgca
     5041 acaaagtcgt tttagataat gcgaaaaaac agcctttccg gtactctacg gcggttttat
     5101 cgtcctgtag agaaattatg atgtccacca cgttatttaa agatttcacc ttcgaagccg
     5161 ctcaccgctt accacacgtc ccggaagggc ataaatgtgg tcgcctgcac gggcattcct
     5221 ttatggtgcg actggaaatt accggggaag tcgatccgca tacgggctgg attatcgatt
     5281 tcgctgaact gaaagcggcg tttaagccaa cttatgagcg cctcgatcac cattatctca
     5341 atgatattcc aggtctggaa aacccaacca gcgaagtttt agcaaaatgg atttgggatc
     5401 aggttaaacc cgttgtgccg ctgttaagtg cggtgatggt aaaagaaacc tgcaccgcag
     5461 ggtgtatcta tcgcggcgaa tgataagagt gtgtcggcgg tcaatttccc ttaagtaacg
     5521 ctatgttagg gtgttgtgtt ctggatatct ggggcatgac atggaagacg actgcgacat
     5581 tattattatt ggtgccggta ttgcaggcac cgcttgcgcg ttacgctgcg cgcgagcggg
     5641 tttatccgtt ttgttactgg aacgcgctga aatccccggc agcaaaaatc tttccggcgg
     5701 gcggttatat acccatgcac tcgcggaact cctcccgcaa tttcatctga ccgcgcctct
     5761 tgaacgacgc atcactcacg aaagcctttc cctgttaacg cccgatggcg taacgacgtt
     5821 ttccagctta cagcccggcg gtgaatcctg gagtgtatta cgtgcacggt tcgatccgtg
     5881 gctggttgcc gaagccgaaa aagaaggtgt cgaatgcatc cccggagcga cggtggatgc
     5941 actgtatgaa gaaaacggca gagtctgtgg cgttatttgt ggtgacgata ttctccgcgc
     6001 ccgttatgtg gtgctggcag aaggtgccaa cagcgtcctg gctgaacgtc acgggttagt
     6061 gactcgtcct gctggcgaag cgatggcgtt ggggatcaaa gaagtgctgt cgctggaaac
     6121 atccgctatt gaagaacgtt ttcatctgga gaataacgaa ggcgcagcgt tgctgttcag
     6181 cggcggaatc tgtgatgact tacccggcgg cgcatttctt tatactaatc aacaaacgct
     6241 ctcgttaggg attgtttgcc cgctctcttc ccttacgcaa agtcgtgttc cggcaagcga
     6301 gctgctgact cgctttaaag cgcatccggc agtgcgcccg cttatcaaaa acacggaatc
     6361 actggagtat ggtgcgcatc tggagccaga aggtggcttg cacagtatgc cggtgcaata
     6421 cgccggtaac ggctggctgc tggtgggcga tgcgttgcgc agttgcgtca ataccggaat
     6481 ttccgtgcgc ggcatggata tggcgctgac tggcgcacag gcggcggcac aaacgctaat
     6541 aagcgcctgc cagcaccgcg agccgcaaaa tctgtttgcg ctttatcatc acaacgtaga
     6601 gcgcagcctg ctatgggatg ttctacaacg ttatcagcat gttccggcgc ttttgcaacg
     6661 ccctggctgg tatcgggcgt ggcctgcgtt aatgcaggat atttcccgcg atttatggga
     6721 tcagggtgat aaacctgttc caccgctgcg ccagttattc tggcgtcatt tacgtcgtca
     6781 tggcctgtgg catctggcgg gcgatgttat caggagtctg cgatgtctgt agcccataat
     6841 ctctggcgcg ttgctgatgc gccgcacatt gttccggctg actccgttga gcgtcagatg
     6901 gtacaacggt tgattaacgc ctgtccggca ggtctttttt cgctcacacc ggaaggtgac
     6961 ttacgcgttg attatcacgg ctgcctggag tgtggcacct gccgtttgct gtgcgacgaa
     7021 tccacactac aacagtggcg ctatccgcct tccggattcg gcatcaccta ccgctttgga
     7081 taaataataa ggataattta tgcccctctt acacctactc cgccagaatc cggtgattgc
     7141 tgccgttaaa gataatgcca gcctgcaact ggctatcgat tctgaatgcc agtttatttc
     7201 cgtgttgtac ggcaatatct gcaccatcag taatatcgtt aaaaagatta agaacgccgg
     7261 gaaatatgct tttattcatg ttgatttgct ggaaggcgcg tcaaataaag aagtcgttat
     7321 tcagtttttg aaactggtga ccgaggcgga cggcattatc agtaccaaag cctcaatgtt
     7381 gaaagcggca agagcggaag gttttttctg tattcatcgc ctgtttattg ttgattcaat
     7441 ttcgtttcac aacattgata agcaagttgc gcaatcgaat ccggattgta ttgagatcct
     7501 gccaggcggt atgcccaaag tgctgggctg ggtgacagag aaaatccgcc aaccgctgat
     7561 tgccggtggg ctggtgtgcg atgaagaaga tgcgcgtaat gcgattaacg cgggtgtcgt
     7621 ggcgctttcc accacgaata ccggggtctg gacgttagcg aaaaaattac tttgacggga
     7681 taatcgtaac caattgaatt tggtttgatt tgtaggccgc acgccacatc cgacattcag
     7741 cgcctgatgc gacgcttgac gcgtcttatc aggcctacaa gtccccaggc cagaaccgta
     7801 ggcaggatca tccggcaaca cgttattggc aatctgcgtg aatgtttgtc accagtgcct
     7861 ccagcaccac tttccagtca tcaaccacgc caacatctgc ctgtgaaaat accgcagcgc
     7921 tggcgtcatg attaatcgcc accacaaact tgctattgcg tacgcccgcc attaacgctg
     7981 ctgcgccaga agcgccaaca acaatacaca cctcaggtgc cagcagatgt ccggaaatac
     8041 caatcacttt ttcagcatca acgccaccgt tcatcactcg cgcccgactg tagcccactt
     8101 cggctcccag cttttctgcc agcatagcga tttcctggtt atctgcctct ccgccctgcc
     8161 ccaccaccag cacccgtctg gcctcagcta atgggtcgcg agtaacattt ttcagatcct
     8221 ctgtgctaac cagccagtcc ggaagcgcac ccgggacaat gttcagttgt tgcattccag
     8281 agggcaacgt ggcgtttttc gctgctcccg cctgacgcgc cagggaaagg catagcgggc
     8341 gtttttctgt ttgcagcgtc gccgttagcg catttcccca gtgggatttg cgcacgctta
     8401 cggttggaat atctaacgaa gtcacctggc agatgcttgc accatgtaaa cgccaggcca
     8461 gccgggtcga cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg tccgcaggcg
     8521 ttcgctgcca ctgttccacc aacgcatcca gcacctgttc cgccactaca ggctgaggtt
     8581 caatctgcca gtgcgccagg gtacaaccgc tgaaatcctg cgccgccagc cagcttgcta
     8641 ttgcggcatt ctcctgattt attgtcacaa ttgcgatatt catggctgca tcctttggcg
     8701 taaatagtcc tgccatagct tttgagcttt ttctgctacc gtttgcccat caatcagcgt
     8761 cgctccgcgt cgttgctctg ctcttgccag ttgcagacac tgcatcgctg gcatttcggc
     8821 agcaacagtt ttacggataa tttctgcttt ccccgcagcc attcgctggc gcattcccgg
     8881 tactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca agcgaacccg
     8941 gcaacaacgc aacccatgtt cagtacgctg ttcgagggtg ataaacagcg cgtcgagagt
     9001 gaaacgctcc acctgggtga agcagggcca gcccagcatt tccgccagca aaaatggcgt
     9061 ttgcccattc tgcccttcgc tgctttggca gccggtaatt atcagatcca gcggattctg
     9121 atgctgccat tcggcaatat ggcgagcgac aaattccggc gcaaagcgca gatctgccgc
     9181 cgtctccagc aatacagctt cctcaaaccc aagagccatg agatagcgca gccagtgcaa
     9241 tgcccgttca tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt tttttctctg
     9301 cgccaacagc agcgcggcag cggcctgttc atcagcaccg agtaaacttc gcagtagcga
     9361 aatatccggt ccgcttttac cctgagccgc cgcctgccac tctttttccg ccagcattcc
     9421 ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcct acatttcccg
     9481 ccgccaccag cgggagggct ttagtttctg gtgcccatag ccaggtcaca acaaaatcaa
     9541 ccaacaacac tgtcgccagg agcaataagg tgacttgcat tccccactgc gccagcaccc
     9601 acggcagcag gccagtactt accgccgcgc caagtcgact catggcggtg gcaaaaccga
     9661 cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc accagattac
     9721 tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat gaactgccgg
     9781 aaggcaaaca ggccatcact accagcgttg ccgccagcag caaaaaactt cccagcaaaa
     9841 atttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcgccc acaattaaca
     9901 acgcattaag catcaggctg gcagtcagcg catcttccag accaatagtc tgggcgatag
     9961 ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag aagacgctgt
    10021 taaacgccgt gcgccgccag taacgcgaag agaacaaggt tttgatgtgt ttatgggtcg
    10081 ccgttaccac ttcatcgccc agtaaaacat ggggaccaaa atagcgatgc acgatagcgt
    10141 gagcttctgc aaaacgcccc tggcgcagta gccagcgtgg tgattctggc gttccccagc
    10201 gtaataacgt aatcaacaac gcgggcagag ctgccgatgc cagtagccag cgccaggcct
    10261 ccgggttttc ggaaataaag tgatgtccgg caatacttgc cagcacatag cctacggtcc
    10321 acaccacgct gaatgcgccc agcaaaatac cgcgatggcg gcgcggggaa aattcagcca
    10381 gcaaggtgtg acctactgaa taatcgcctc ccagaccaat gccaatcaaa atgcgcagtc
    10441 caataagatg ctctggcgtg gtggcaaaaa attgtaaaaa cgaagcaagc gtaatcagca
    10501 aaaagctgaa ggtgaagatt ttttgccgac caatatggtc ggagatccac ccaagaacca
    10561 ggctgccaag gaacaaacca aggagcgccg agccgccgat cattcccgcc ataaacggcg
    10621 tcagttgcat ggcgggcgta agctgaataa tggcgtaacc aatgacgccg agaacataac
    10681 cgtcggtcag gtgtgcgccg aaagtgagcg cagcaatgcg gcagtgaaaa cggttaagcg
    10741 gtaaatcatc cattcgcacc ggtgaagtgt tcatctttac cccttcattg tccggtaata
    10801 agaacggctt ctcgcctgag aagccgaaaa ggcaacgccc cgtcatcgcg gaacgctgcc
    10861 tgacacatta tttttcaatc ggatagatag tacccgtatt cataatgcca ttaggatcga
    10921 actgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagt ttgctccagt
    10981 gaacgcgatg tttaccgata ccgtggtggt gcaccatcga accgccgagg cgaatggttt
    11041 cttcacagat gatcttgttg agcggattgt ggtacttgtc gatttcctct tccggcttac
    11101 agtcaacgac gttgtaatcg tagacgaagt acatgttggt gccgttctga tagctatgag
    11161 aggaatgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcagta cgaatacggt
    11221 taataacgct ttcgtagatt tcgtggatgc agctccagca gccggacact tcagtggtaa
    11281 agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta tccggtcccc
    11341 agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cacgcgctgg cattgcgggt
    11401 agcgggcaac gatttccgca atcccttcgc ccgtcgcctt cgcaatgcga gggttacctt
    11461 cagccataaa gatcagcacg cattttccgt ctgcaaaatg ggtgaagtgt tgggtgccat
    11521 cttcagcgtc atacaaacga gcgatcgacg gacgataccc ttccaccatg acttcacgca
    11581 ggatgttgaa gtcggttttc atgtcttcca ggatatagcc gtagaagagg ttgttttccg
    11641 gggtgaattt aaagattttc actgttactt cagtgatata gcaaagtgca ccttcgttac
    11701 cgatgatgat gtggcgaatg tccgggccag ccgcgcggcg tggcacgttt ttaatgcgtg
    11761 tgacggtgcc atcagccaat actgcttcca gaccaacgac catatcttcg attgcgccgt
    11821 agagtgtgga gaactgcccg atactgcggg ttgctaccaa gccgcccatc tgcgccagcg
    11881 gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgca ttttccagca
    11941 cctccagcgg aaccccacat tgcgccgtcg cctgcatatt ctcaatatca atattaatga
    12001 tttgattcat ggcggagccg tcaagcacca ccgagttttc cacaacggtt tccagcccac
    12061 cttcagtggc ggaagcgccg gtacgcggca caccgttaat tttgtgcgca ttcataaaat
    12121 tcagcacacg ggatacttgc tctgtggaac cgagttttac gaccgctgcc ggaatcggca
    12181 aagtataaat gccatgaata tccggaaatt tacgaaaacg gtcaatactg ttcttcttta
    12241 atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagt tggtcgacaa
    12301 tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg aataatcatt
    12361 aataagagaa aacagaaacc accgttatgg attagcgcac taaataacca ccattgacca
    12421 ctaataaatg cccattgaca taatttgaag ccggacttgc gaggaataca gctgcgccca
    12481 ttaaatcctg agtatcgccc caacggtttg ccggaatatg atcaagaacg cgctgattgg
    12541 tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca ggagcaatac
    12601 catttacctg aatattatat tgacctagtt catcacaata agctttggtg aatccggcaa
    12661 gagcatgttt agtggcagaa tatgcaggtg accattgtcc acctaagtaa gagaacaatg
    12721 aacagatatt aataattttg ccgcttttct gcgggatcat aatttttgca gcttcatagc
    12781 ttaactcgaa tgcggcggtc aggttcacat caatcatcgg atcccagtcg gcacgaccga
    12841 agtccagcac cttattcagc ttacaaatac cggcattgtt taccagaata tcaactgtac
    12901 cgaaacgctc acagcaagca gcgataatct tctgcggcgc gccttctgcg gtgatatcca
    12961 cctgcatgaa gtccacctca acaccctgtt tttcaatcat ttcctttgtt tcgccgttat
    13021 ctttcaatca tttcctttgt ttcgccgtta tctttgacga aactaggaat aaagatattt
    13081 gcgccagctt tggccaacgc catggcaaat gcctggccta aaccgctatt cccaccggta
    13141 acaattgcgg ttttaccttt cagtgagaaa aaatccattg agaacgcatt gagagattcg
    13201 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa gaaaggtaac ttcccccgcg
    13261 agagggaagt tacctttctc attatctctg gtaactggag ataattttta gcataacggc
    13321 ataactgcta atgtgatcgt aatcacagtg tgatattcgt attgtcaaat agttttaaaa
    13381 agcagaagcg tgattactct cacacaacac ctctgctttc catgttattt cttattgcaa
    13441 ttactagaga cctgagaaaa gtaatccacc tgaaagggcg gcttactttt ctcttttttt
    13501 tgtctattac aaatgggaat acgatatgca acacaactca tatcgccgtt ggataaccct
    13561 cgcgataatt agttttagcg gcggcgttag ttttgacctg gcttatttac gttatatttt
    13621 tcaaattccc atggcgaaat ttatgggatt cagcaatacc gagataggtt taataatgag
    13681 tacctttggt attgcggcta ttattcttta tgcccccagc ggcgttattg ccgataaatt
    13741 ttcacaccgc aaaatgatta cttccgcgat gatcattacc ggattactgg gtctgttaat
    13801 ggcaacgtat ccaccgctgt gggtaatgct ctgtattcag gtcgcctttg cgataacgac
    13861 gattttaatg ctgtggtcgg tgtcgattaa agccgcctcg ttgctgggcg atcatagtga
    13921 gcaagggaaa attatgggct ggatggaagg gctgcgcggc gtcggtgtaa tgtcgctggc
    13981 ggtgtttacc atgtgggtct tttctcgctt tgcaccggat gacagcgcca gcctgaaaac
    14041 agtcattatc atctacagtg tggtttacat cttgttgggg attctgtgct ggttttttgt
    14101 tagcgataac aacaacctgc gcagtgccaa taacgaagaa aaacagtcat tccagcttag
    14161 cgacatcctt gccgttttgc gtatcagcac cacctggtat tgcagcatgg tgatttttgg
    14221 tgtcttcaca atctacgcca ttctgagtta ctccaccaac tatctgaccg aaatgtacgg
    14281 catgtcgctg gtggcagcga gctacatggg gattgtgatc aacaaaatct tccgcgcgct
    14341 gtgcggccca cttggaggca ttatcaccac ctacagtaaa gtgaaatccc ctacccgcgt
    14401 gattcaaatc ctttccatca tcggtttgct ggcgttgaca gccctgctcg tcacgaactc
    14461 taacccccaa tcggtcgcga tggggattgg cctgatttta ctgctgggat tcacctgtta
    14521 cgcctcacgc gggctgtact gggcctgccc cggcgaagcg agaacaccgt cttacattat
    14581 gggcaccacg gtaggtattt gttcggtgat tggattcctg ccggatgtct tcgtttaccc
    14641 gattatcggc cactggcaag acaccctgcc cgctgcagaa gcctaccgca atatgtggct
    14701 gatgggcatg gcggcgctcg gcatggtgat tgtctttacc tttttgctgt tccaaaaaat
    14761 tcgtactgct gatagcgccc ccgcaatagc tagcagcaag taagccagga aaattacagg
    14821 agaaaaatca tgtcgaaaaa atacatcata gggattgatg gcggaagtca gagcacaaaa
    14881 gtggtgatgt acgatctgga aggtaatgtg gtttgcgaag gtaaaggctt attacagccg
    14941 atgcacacgc ctgatgccga tactgcagaa catcctgacg acgatttatg ggcatcatta
    15001 tgttttgcag gtcacgattt gatgagccag tttgccggga ataaagaaga tattgttggc
    15061 atcggcctgg gctccatccg ttgctgccgt gcgttattga aagccgacgg cacgccagct
    15121 gcgccgttga ttagctggca ggatgcacgc gttacacgcc cttacgaaca tacgaatcct
    15181 gacgtggcgt atgtaacctc tttttcgggt tatctgacgc atcgcttaac cggcgaattt
    15241 aaagataata tcgccaacta ttttggtcag tggccggtgg attataagac ctgggcatgg
    15301 agcgaagatg ccgcagtcat ggagaagttt aatatccccc gtcatatgct gtttgatgtg
    15361 caaatgccag gcactgttct tgggcatatc acacctcaag ccgcgctggc gacgcatttc
    15421 ccggcaggac tgccggttgt ttgtaccacc agtgataaac cggtagaagc tctgggagcc
    15481 ggattactgg atgatgaaac tgcggtaatt tctcttggca cttacatcgc attgatgatg
    15541 aacggcaaag ccttgccgaa agatccggtg gcgtactggc cgattatgtc ttctattccg
    15601 caaacattgc tgtatgaagg ttacggtatt cgcaaaggca tgtggacggt gagctggctg
    15661 cgcgacatgt taggcgagtc gttaattcag gatgccaggg cgcaggatct ttcaccggaa
    15721 gatttactca acaagaaagc ctctagtgtg ccacctggct gtaatgggct gatgacggtg
    15781 ctggactggc tgaccaatcc gtgggaaccg tacaaacgcg ggattatgat cggctttgat
    15841 tccagcatgg attacgcatg gatatatcgt tcgatattgg aaagcgtggc gctgacgctg
    15901 aagaacaatt acgacaatat gtgtaatgaa atgaatcact ttgcgaaaca tgtaatcatt
    15961 actggcggcg gttcgaacag gatctgttta tgcagatttt tgccgacgtg ttcaaccttc
    16021 cggcacgccg taacgccatt aacggttgtg caagcctggg ggcagcgatt aatacagggg
    16081 taggtctggg gctatacccg gattacgcaa cggctgtcga taaaatggtt cgcgtgaaag
    16141 atatctttat accgattgag agcaatgcca aacgctacga cgcgatgaat aaaggcattt
    16201 tcaaagacct aaccaaacat actgatgtga tcctgaaaaa atcgtatgaa gtgatgcatg
    16261 gggaattggg gaattgggga atgcggattc gatccagagc tggtcgaatg cgtgaataag
    16321 gtaggccggg cggaaaactg cccggcctga acatacctga atggttatcc ccgctggcgc
    16381 ggggaacata agttcgacaa cttaaaaaat gataacaagt ccggtttatc cccgctgatg
    16441 cagggaacat aataaaaact ttaactagtt cggtttatcc ctgtttttac aaggaa
//
LOCUS       DAZOZD010000078        16381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOZD010000078 DAZOZD010000000
VERSION     DAZOZD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.078"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            258..758
                     /gene="tssB"
                     /locus_tag="ACHUPB_002053"
     CDS             258..758
                     /gene="tssB"
                     /locus_tag="ACHUPB_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR0758869.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            793..1017
                     /locus_tag="ACHUPB_002054"
     CDS             793..1017
                     /locus_tag="ACHUPB_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758870.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1068..2543
                     /gene="tssC"
                     /locus_tag="ACHUPB_002055"
                     /pseudo
     CDS             1068..2543
                     /gene="tssC"
                     /locus_tag="ACHUPB_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2550..2963
                     /gene="tssE"
                     /locus_tag="ACHUPB_002056"
     CDS             2550..2963
                     /gene="tssE"
                     /locus_tag="ACHUPB_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0758871.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2967..4817
                     /gene="tssF"
                     /locus_tag="ACHUPB_002057"
     CDS             2967..4817
                     /gene="tssF"
                     /locus_tag="ACHUPB_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0758872.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4781..5863
                     /gene="tssG"
                     /locus_tag="ACHUPB_002058"
     CDS             4781..5863
                     /gene="tssG"
                     /locus_tag="ACHUPB_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR0758873.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5888..7168
                     /gene="tagH"
                     /locus_tag="ACHUPB_002059"
     CDS             5888..7168
                     /gene="tagH"
                     /locus_tag="ACHUPB_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR0758874.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7165..7689
                     /gene="tssJ"
                     /locus_tag="ACHUPB_002060"
     CDS             7165..7689
                     /gene="tssJ"
                     /locus_tag="ACHUPB_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR0758875.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7692..9021
                     /gene="tssK"
                     /locus_tag="ACHUPB_002061"
                     /pseudo
     CDS             7692..9021
                     /gene="tssK"
                     /locus_tag="ACHUPB_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            9026..9787
                     /gene="icmH"
                     /locus_tag="ACHUPB_002062"
     CDS             9026..9787
                     /gene="icmH"
                     /locus_tag="ACHUPB_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR0758876.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9796..12579
                     /gene="tssH"
                     /locus_tag="ACHUPB_002063"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9796..12579
                     /gene="tssH"
                     /locus_tag="ACHUPB_002063"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12576..13319
                     /gene="vasI"
                     /locus_tag="ACHUPB_002064"
     CDS             12576..13319
                     /gene="vasI"
                     /locus_tag="ACHUPB_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR0758877.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13324..14736
                     /gene="tssA"
                     /locus_tag="ACHUPB_002065"
     CDS             13324..14736
                     /gene="tssA"
                     /locus_tag="ACHUPB_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR0758878.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14755..>16381
                     /gene="tssM"
                     /locus_tag="ACHUPB_002066"
     CDS             14755..>16381
                     /gene="tssM"
                     /locus_tag="ACHUPB_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFR0758879.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         3752 a         4538 c         4549 g         3542 t
ORIGIN      
        1 ctatatttaa attattgcca taatacacct gaagcgaatt gcacaggttc acacaatcta
       61 tttaacgctt gcagaatacc cctctttacg atcgataaaa agtccttttc ttatttattt
      121 tgaagcgtaa aatgcggggt gatgaaggag tggtgatttt attttcaccg ccattagggc
      181 ggcattgccg atattttttt gcggtacaaa tatgccgtgt tcatggcctg tacgagataa
      241 catgacggat cacttttatg agcaaaaaat ttgaaggcag cgtcgcgccc cgcgagcgta
      301 ttaatatatc gtatgtccca aaaacagatg gccagacggc tgaagtggaa cttccgctta
      361 acatgctcgt tgtcggcgat accggaaata cgcaggaaac atccccactg gatgaacgtc
      421 aggcagtttc cgttaacaag cacaatttcg gtgcagtgat ggctgaagca gccattggcc
      481 tcaattttac tgtccccgcc acgctgaagg gaagcacaac agacgatgaa ctgaatgtgg
      541 cgctgaatat caaatcactg gatgattttt caccagatag cgtggcccgg caggttccgg
      601 aagtgaataa attgctggaa ctgcgtgaag cactgactgc actgaagggg ccgatgggta
      661 accttccggc attccgtacc cagctccagg cattgctgga aaatgaagag tcccgcgagc
      721 agttgctcaa agaaatcggt ctggtgagca ataaataacc accactattt cattaaacag
      781 ggttatccag gcatgacggc acacaaaaat atctccggga cataccacct ctctgttgca
      841 gacattctgc aggtggttta ccaggtatgt ttcagcccgt ccgttgaaat caaccaggat
      901 ggtgtggctg cgctgattac aacactggat cgccgtatca gtgatctatt ggatgagatt
      961 atccattttt gtgagtttca gcagagcgcg tcgcactggc agcgcgtact tcactgatgg
     1021 cgaaaagccg gtttcagaaa cacagctaaa acagaaggat tttcagtatg tctttacagg
     1081 aagaggaact cgtttccagt catgccgggc agcctgagca ggcatcgtcg ctgcttgatc
     1141 aaatcatggc acagacccgt attcagcccg gttcggaagg ttatgacgtt gccctccagg
     1201 gtgtgacagc ctttattgcc agcatcctgc aatccactgc atctgccgaa ccggtcaata
     1261 aactggccgt ggacagcatg attgcagata ttgatgaacg catcagccgc cagatggatg
     1321 tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt ctggcgctca ctgaaaacca
     1381 tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa cgtgcttcat gtcaccaagc
     1441 aggaacttct ggaagacttt gagtttgcgc cagaaattat tcagtccgga ttttataagc
     1501 acgtttattc gtccggtttc ggacagtttg gcggtaaacc catcgctgcc gtgctgggcg
     1561 cttatgagtt caaaaatacc gcgcctgaca tgaaactgct gcaatacgtc agtgccgtgg
     1621 gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc ggagtttatg gggctgaact
     1681 cgtggaccga actgcctaat atcaaagatc tttatgccat ctttgaaggt ccggcctaca
     1741 ccaaatggcg cgccctgcgt gactcggaag attcgcgcta tttagggctt acggcacccc
     1801 gcttcctgct tcgccagccc tattcaccaa cagataaccc tgttaagaac tttaactatt
     1861 acgaagatgt cagccagaac cacgaagatt atctgtgggg taatacggcc tggatgctgg
     1921 catgcaatat cgcagacagt tttgccaaat accgctgatg tccaaacatt atcggtccgc
     1981 aaagcggcgg cgcagtgaaa gatctgccga tgcatctgtt cgaaacgatg gggcagattc
     2041 aggccaaaat cccaaccgag gtgctggtca ccgatcgccg cgaatttgaa ctggctgaag
     2101 agggttttat cacccttacc atgcgtaaag actctgataa tgcagccttt ttctctgcaa
     2161 actcggtaca aaaaccgaag cactttccgg gaaaagatgc agaaaccaac tataagctgg
     2221 gtacgcagct tccttatctc ttcatcatca atcggttagc gcactacatc aaggtgttgc
     2281 agcgtgaaca actggggtca tggaaagaaa ggagcgactt agaaagagag cttaatacct
     2341 ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc tgacgtgcgc agccgcaaac
     2401 ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg cgaacccggc tggtaccagg
     2461 ttgcgctaag cgtgagacct cattttaaat ttatgggggc aaattttgag ctttccctgg
     2521 ttggccggtt agacagggag taacacacca tgataccgga tcgggaaaga accacaggtt
     2581 ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg gcagggaggc agtattcatt
     2641 cgctcaggca atccattcgc cagaacctac gaaacattct taatacccgg tccggtagtt
     2701 gccggggtgc gcctgagctg ggcattgatg aaccggaagg ggctgaaaac ttccgcgaat
     2761 ccatgagtcg ggccatcgag caatgtatag aacgctatga accccggatc tcacacgctg
     2821 aagtgcaggc agtggtttca tccgcctcca gcccgctgga tatgacgttt catattacag
     2881 cctgggtgac atttaacgaa acgcatgagg tgcttgaatt tgatatggca ccgaatggca
     2941 gccagcatta ccgggtggac tgacaaatgg aatttgaaga acgctatttc cgggaagaac
     3001 tcgattacct gcgccagctt agcaagctgc tggcaacgga aaaaccccat ctggcccgct
     3061 tcctggccga aaaagatgca gatccggata ttgaacgcct gctggaaggg gtggcttttc
     3121 ttaccggcaa tctccgccag aaaattgagg atgaattccc agaactgacg cacgggctta
     3181 ttaagatgct atggcctaat tacctgcgtc cggttccggc aatgaccctt attgaatata
     3241 cgccggatat ggataagtct tctgtaccgg tgttaatccc ccgtaatgag cagtttacaa
     3301 ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc tgatgctaaa aaggaggagc
     3361 cgcctccctg taccttcacc ctctgccggg atatctggct gctgcccgtt cgcctggagc
     3421 agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa catcaccttt tcggtcgcac
     3481 cgggaacgga cttccgcacg ctggatctga acaaacttcg cttctggctc ggcaatgacg
     3541 acaactatac ccgtgaccag ctttatttat ggttctgcga atacttgcag ggtgccgacc
     3601 tgactgtggg tgaacagcat attcgcctgc ctgagtttat gctaaaagct gtcggttttg
     3661 agccgcagga tgccatgctg ccctggccga aaaacgtcca cagcggctac cggatccttc
     3721 aggagtattt ctgttacccc gatgcgtttc tcttttttga tctttgtggt tgtccggctt
     3781 tgcctgacgg attgcaggcg gaattcttta ccctgcaact gcgtttttcg cgccctttgc
     3841 ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta ttgcgcacct gccattaatt
     3901 tatttatcca ccatgcagaa gccatcacgc tggacaaccg gcgggcagac tatccgctgg
     3961 ttcccagccg ccattaccca caacattacg atgtattttc cgttaacagt gtggtgagcc
     4021 aggtccagga tatgttcagg aaaaaagatc tggggcgtcc tgtttcgacg caggccgcgc
     4081 gccagtggcc agcctttgaa agtttcagcc atcagataga atacagccgg aagcgggaag
     4141 tggtgtactg gcatcaccgg accaaaacat ccctgttcca tcgcggcttt gatcataccc
     4201 ttgcctttat acatgctgat ggcagttatc cgtcagacga atctctgctc agtaatgaag
     4261 tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc gtcacaaatt cgttccggcg
     4321 atatcaccgg cacaaccgga aaaaatgcgg ctgttgcttc atttcgcaac attacccgcc
     4381 cgacgcaacc actctggccg gtcattgatg gcagcctgca ctggccccta ctctccgcca
     4441 tgaacctgaa ttatctgtca ttactggata cggacgcgct gaagcaggtc atcgccaact
     4501 ttgatcgcca cgcaatccat catccgcaga cggcacggct gtcacaacaa aagctggatg
     4561 ccattgagcg tctggagacc cgccccgttg atcgcctgtt tacgggtatt cccgtccggg
     4621 ggctggcttc cacgctgtat ctgcacccgg agccgtttgt ctgtgaaggg gaaatgtatc
     4681 tgctcggtac ggtgctttcg cattttctgt cgctgtacgc cagcgttaac tcattccaca
     4741 tgctgaccgt tgtgaacaca gaaagccagg agacatggaa atggacggaa agaatcgggc
     4801 agcatcctct tatctgagtc cgggaaatcc cccggcagat aaagagcaga atgatccgct
     4861 ggcacaggtt ttccataatg cctgctctta caattttttt gcgatggcgg agctgttgca
     4921 ccgcctggca aagggtgaaa aggggacgcc agaattatcc ctgcgcgacg atccggcaca
     4981 ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc ccgtgcagtg atatcagtgc
     5041 gctgaaacgg gatacgtcag gggcattcag gatgaccacc acctttatgg ggttacaggg
     5101 gagccagtcg cctctccccg gttattacct cgctcacctg gcctggaaag cggtacatga
     5161 acaaagtccg gtaggtgatt ttctggatat gttcagccac cgcctgaccc agtttgtctg
     5221 gcatatctgg cgtaagtacc gctatcacat cagttttcgt aacggcggtg tggatgcgtt
     5281 ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc cagcttcgcg ataagctggc
     5341 gattaatcac agcaaaatgc tggcgtattc cgggatcctg gcaaatccgg gacgatcccc
     5401 ggaaattatc tgtggcctgg tgtcccactg ctttgatttg tcagaagtga cactgcaaaa
     5461 ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa aatagtctgg ggagctattc
     5521 tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg ggcaactttg ttctgggtac
     5581 acgagtgccc gatctcagcg gaaaattcca gctcagtatt accagcctga cccgaaaaca
     5641 gttcctctct tttctgccgt ccggcgaaaa cttcctgcca ctgacgatgt tcgtgtcctt
     5701 catcctgcgc gatcagcttg cctgggactt acatctgggg ctggcgccgg agcaggtggg
     5761 cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc tggaccagct tcctcggcac
     5821 accggaagaa cgaccctcag tcaccatcag ggtacggtcg taaattatga taatcacgga
     5881 gcagaatatg ccggaagaga aactacagac gctttcattg caggtcatta acggcagtga
     5941 gctggaaagc gggcgggcgg cgcgctgtct gttcacacag cagggaaatg tcggccatgg
     6001 ccccgaatgc cactggtcgg tacaggatcg tcagcagagc attccggccc aggcttttac
     6061 cgttatcctg cacgatggca cattttgtct gcgcccgcag acggcacaac tgtggctgaa
     6121 tcaggcaaaa gtcacagcaa catcagacct aatacagttg cgccagggcg atgagatcca
     6181 gatcggacgg ctgatggtga gggttcatct gaaccgggga gatattcccc attacgatga
     6241 ggaaatggcc actcccgaaa ccatcgttac caatcgcgat atgctcacgg ataccctgct
     6301 atcaacggag ggtgcgccac actatccggg aatgactcac cggcaccagc ttgcagacac
     6361 cgtggtaaac ggtttttctg ccgatccact ccaggctctt cagtccgaaa gcctgattac
     6421 cacgggcgat ccgctttcag gcattgcggc tgtccggcca tcggcaccgc tgtccgatcc
     6481 ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt atgccagccc
     6541 cggcgatcgt aatgacgacg tttctgcggc agaaatggca caacgccacg ttgcggtcac
     6601 ccccttactg cgcggtcttg gcggctcgct taccatgagc aattccgacg atgcggatga
     6661 ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc tcgatttgca
     6721 gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata acccgctgcg
     6781 cctgaacatg gattacgcca ccgcgctcga cgtaatgttt gccgaaggta aaagcccggt
     6841 acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc accacgaaga
     6901 agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg ctggatgctt tctcaccaca
     6961 aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc agccgctgga
     7021 tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct ccgatcgcca
     7081 gcaggggttt gagatgctgt ttaacgaggt ctacgcccag gtctatgacc gggtgcttcg
     7141 tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg cgtgctgatg
     7201 tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gtgaggtgat caaaaacccg
     7261 gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac cctgctgacc
     7321 gaaccggaca ccaataccaa tgcggaaggt gaatcagccg ccgtggatgt gcaactggtg
     7381 tacctgactg acgactcaaa attgctggca gccgactatg accagatagc cagcacgccg
     7441 ctaccggatg tgctgggtaa aaactatatc gaccaccagg atttcaacct gttgccggac
     7501 accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa cgcagtttat tggtgtcgtg
     7561 gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt ggaaggaatc
     7621 ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat gaaaaaagag
     7681 gatgaataat tatggcaacc acccgcaaca aggtgatgtg gcaggaaggg atgctgatgc
     7741 gtccacacca tttccagcag cagcagcgtt acaacgacta cctggataac cagcgtttcc
     7801 gggccatgaa tgatttatcc tggggattta ccgaactcac cctcaacaat gaactgctgg
     7861 cgcagggtaa gatcatgatt gacagcgcgt caggcacact gcccgacggc accgtctttt
     7921 ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaaaatttt ccggacgaaa
     7981 gaagccgcaa tatctacctc gctctgccgg tcgccagtga tgtgagaaat gaaatcagcg
     8041 acggacggcg aatcgggcgt taccggctga attatgccga tgtccgggat ttgcattcag
     8101 aagaaggtga cgcgcgaaca ttgacgctgg gtcaactgac gccgcgcatt atgagcggtg
     8161 cagaagatat gagcgcctat attacgctcc cgctttgccg tatcagcgat cgccgccgat
     8221 ggctccctga cgttggatga cgattttatc ccctcctgcc agaatattca ggtcagtaag
     8281 aaactgcgtg tttatctcaa agaggtacag ggggccattg gcggacgggc aagcgatctg
     8341 gcaaaccgta ttggctctcc ggcgcagagc ggcatcgcgg atgtggcgga atttatgatg
     8401 ttgcagttac ttaaccgtaa ccagacccgg tttacccatc acgcccgtcg atcccagctc
     8461 cacccggaag atttctacct tgatcttgcc gggttgctgg gtgaactgat gacctttacc
     8521 gagccgtcgc gcctgccctg cccgcttgat gtgtatgatc atcatgacct gaccaaaata
     8581 tttaaaacgc tgttaccgga agtcaaacgg gcgctgcata ccgtactgtc gccaagagcc
     8641 gtcaatctgc cgctgcatct gcgtgacggt atctggcagg ccgatatcca tgacacagaa
     8701 ctgctgcaat ctgccacctt tgtgctggcc gtggcggcaa acatgccggt cgatcagatc
     8761 cagcgccagt ttatccagca gtcgaaaatt tcctcgccgg aaaaaatccg caatatggtc
     8821 agtgtgcaga tccccggtat tccattgcgt gcccttatgg tggctccccg ccagcttcct
     8881 taccattccg ggttcagcta tttcgaactc gacaagagtg gccaggcctg gacagaaatg
     8941 gctgccgccg gggccgttgc actgcatgtt tccggcagtt tcccggatct gaacatgcaa
     9001 ctgtgggcga taagagggta agacgatgga tgaggggtca ctgtctctgc caccatttac
     9061 cggacacgac gaaaaaagcc agcgcaacta ccatctcgcg cttcgtggca acagcctgaa
     9121 tccgatgatc gacgcggcca cgccgctact cggtatggta atgcgccttt caaccatgaa
     9181 cagccagacg atgccggagc atctttttgc tcaggtggtg acagatgttc aggccgtgga
     9241 gcaactgctt caggagcagg gctacgaacc gggcgtgatt atctcgtttc gctacatcct
     9301 ctgcacgttt attgatgaag cggcgctcgg taacggctgg tcaaacaaga acgagtggat
     9361 caagcagtca ttgctggtcc atttccacaa cgaagcctgg ggtggcgaga aagttttcat
     9421 cctgctggag cgcctgatcc gtgaacccaa acgttaccag gatctgctgg aatttctctg
     9481 gatctgtttt tctctgggat ttcgcgggcg ctacaaagtg gcagcacagg atcaggggga
     9541 atttgagcag atctaccgcc gtctgtacca cgttctgcat aaactgcgcg gcgatgcccc
     9601 tttcccgctg ttacaccagg acaaaaaaac ccagggcggg cgttatcagc tcatcagtcg
     9661 cctgactgtt aaacatattt tttgtggtgg cgttgtcgtg ctggcgttgt tttacctgtt
     9721 ctatctgttg cggctggaca gccagacgca ggacatcctg caccagctaa acaaattact
     9781 tcgataagga cgtttatgat ccagattgat cttcccacgc tggtaaaacg gctgaacctg
     9841 ttctcccgcc aggcgctgga gatggccgcc tctgaatgta tgagtcagca ggcagcggaa
     9901 attaccgtca gccatgtgct cattcagatg ctcgccatgc cacgcagtga cctgcgggtt
     9961 attacccgcc agggcgatat tggcatggaa gagttgcgcc aggcgctgac ggtagagaac
    10021 tacacaaccg cccgttctgc ggacagctac ccggcgtttt ccccgatgct ggttgagtgg
    10081 cttaaagagg gctagctgct ggcgtcggct gagatgcagc acagcgaact gcgcggcggc
    10141 gtgttgctgc tggccctgct gcattcgccg ctgcgttata taccgcctgc tgccgcccgg
    10201 ctgttgaccg gcattaaccg tgaccgtctg caacaggact ttgtgcagtg gacacaggag
    10261 tcggcggaat cagtcgtgcc ggatgcagac ggtaaaggcg caggcacaat gacggacgcc
    10321 tctgacaccc tgcttgcccg ctatgccaaa aacatgaccg cagacgcccg taacggcagg
    10381 cttgacccgg tactgtgccg cgaccacgaa atcgacctga tgatcgacat tctctgccgc
    10441 cgccgtaaaa acaacccagt ggtggtgggc gaagcgggcg tgggcaaaag cgcactgatt
    10501 gaagggctgg cgctgcgcat cgtggcaggc caggtgccgg acaagctgaa aaacaccgat
    10561 atcatgaccc ttgacttggg cgcattgcag gccggggcgt cggtgaaggg tgaattcgaa
    10621 aaacgtttca aagggctgat ggcggaggtc atttcctccc cggtgccggt cattctgttt
    10681 atcgacgaag cacataccct gattggcgcg ggcaaccagc agggcgggct ggatatctcc
    10741 aacctgctca aaccggcgct ggcgcgcggc gagctgaaaa ccatcgccgc caccacctgg
    10801 agcgagtaca aaaaatactt cgaaaaagat gccgccctgt cgcgccgctt ccagttggtg
    10861 aaggtcagcg aacccaacgc tgccgaagcc accattattc tgcgcggtct gtcggcggtc
    10921 tatgaacagt ctcacggcgt gctgattgat gatgacgcct tgcaggccgc tgcgacatta
    10981 agcgagcgtt atctctccgg gcgtcagtta ccggacaaag cgattgatgt gctggatacc
    11041 gcctgcgccc gtgtggccat caacctgtcg tcgccgccga agcaaatctc ggcgctgacc
    11101 actctgagcc accagcagga ggcggaaatt cgccagcttg agcgcgagct tcgcatcgga
    11161 ctgcgtaccg acacatcacg gatgaccgag gtgctggtgc agtatgatga aacgctgacg
    11221 gcgctggatg aactggaagc ggcctggcac cagcagcaga cgctggtccg ggagattatc
    11281 gcgctgcgcc agcagttact gggcgtggca gaggacgatg cggcgccgtt gccggacgca
    11341 gataccgtgg aggatacgca gccagagtca gagtcagagt cagagtcaga gtcagagcag
    11401 gataataccg gtgccgaacc ggctgatgag gccgacagag aacaaccgga agagaccgct
    11461 gaaacagttt ccccggtaca gcggctggcc cagctcactg ccgaactgga cgccctgcat
    11521 aacgaccggt tgctggtctc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc
    11581 gaatggaccg gcgtgccgct caaccgcctg tcgcagaatg aaatgtcggt catcaccgac
    11641 ctgacgaaat ggctgggtga caccatcaaa ggccaggacc tggcgattgc cagcctgcat
    11701 aagcatctac tgaccgcacg cgccgacctg cgtcgtccgg gacgcccgct cggtgcgttc
    11761 ctgctggctg gccccagcgg tgtgggtaaa accgaaaccg tcctgcaact ggcagagctg
    11821 ctctacggcg gtcgccagta cctgaccacc atcaatatgt ccgaattcca ggagaaacac
    11881 accgtctcgc ggctgattgg ttcgcctccg ggctacgttg gctacggtga aggcggcgta
    11941 ctgaccgaag cgattcgcca gaaaccctac tcggtagtac tgctcgatga agtggaaaaa
    12001 gcgcacccgg atgtgctcaa cctgttctac caggcgttcg ataagggcga aatggcagac
    12061 ggtgaaggcc gcctgattga ctgcaaaaat atcgtcttct tcctgacgtc caacctcggc
    12121 taccaggtaa tagtcgagca tgccgatgac ccggaaacca tgcaggaagt acagtatccg
    12181 gtgctggccg acttcttcaa acctgccctg ctggcgcgta tggaagtggt gccgtatctg
    12241 ccgctgtcga aagagacgct cgccaccatt atcgccggaa aactggcccg tctggataac
    12301 gtgctgcgca gtcgctttgg tgtagaagtg gtcattgaac cggaagtgac ggacgaaatc
    12361 atgagccgcg tcacccgcgc ggaaaacggc gcgaggatgc tggaatcggt catcgatggc
    12421 gacatgctac cgccgctctc gctgctgctg ttgcagaaaa tggcggctaa cacggcgatt
    12481 gcccggattc ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag
    12541 aacgacgagt ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc
    12601 agacaggatg gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag
    12661 aagcatcgtc aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga
    12721 cagccacgtt acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg
    12781 actgctatga ccgcctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca
    12841 aagccggttt tgtgggcgag gcctggaccc gcgccacaga gcaggaaaaa cgccgcgagg
    12901 gcaacaccac agaactgctg gtgacgcagg tgccggggga gcgcccgacg gtggtgataa
    12961 ccaccccggc tatcggtcat gttccgccac gcccggtgct gatgttcagt tgcgtggaca
    13021 atatcacccg gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca
    13081 ctctgaatgc cgacaaccgg gcattgcgca gccactggtt tgtgcgcgag aacggcaccc
    13141 tgctggagtc cagccggggg ttatccggga ttgatgaaat taagcaactg ttcggcgcaa
    13201 aaacgctgac ggtggatacc ggcgcagaca atgccgccgg aaagctgacc tttaacattg
    13261 acgggctggc aagggcgatt gctcccctgc gcgatgcctg ccactgggcg ggagaataaa
    13321 aatatggcaa tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg
    13381 ccggaagaga aactggcatc ggcgctgaaa gatgacaacc cgaactggga gtatatcgac
    13441 ggcgaaatcg tcaagctggg atcgctggcc cacagtcaac tggatatccc ggaactccag
    13501 cgccgggggc tggtcattct ggcttcggag agtaaggatt ttcgtctgct ggcgcatctg
    13561 ctaagaaccc tgcaacacgc cggtgatcct catctcgcat tgcgcctgct ggcgctgtat
    13621 gtggagcatt actgggcggt ggctgcgccg cagaatatgg cgcataaaaa acgctttgcc
    13681 agccaggtca ttaaacgctt tgaaacaggt attgaagttt tttcacagaa cgccgccaca
    13741 gcgcagcgcg atgcgctgtc aggggaactg gcaaaactgg cgcaatgctg gcagtcacat
    13801 aacgccccgg aactggcaca ggctactgat gatctttttg ccctgtacca gcgtgcgttt
    13861 aatcgtgcgg caccggctcc tgtccccact ccggcggcct ccggtagttc accacagacc
    13921 accgccacgt ctgaaagcgg cgtgacgcaa cccagtgctc cggctcccca aatcgtcatc
    13981 gacagtcacg acgacaaagc ctggcgcgac acgctgttaa aagtggcggc cattttatgt
    14041 gaacgccagc cggattcacc gcagggctac cgtctgcgcc gccatgcgct ttggcagaac
    14101 atcaccagta cgccccaggc ggaaagcgat agccgtactc cactggctgc ggtctccgcc
    14161 gatatggtgg cagattacca tgctcagctc ggtagtgccg atatggcgct gtggcagcag
    14221 gtggagaaaa gcgtactgct ggctccttac tggctggatg gtcactgcct ttctgcacag
    14281 acggcgctgc gtctgggtta caaacaggtg gcagacgcca tccacgatga ggtcatccgc
    14341 ttccttgagc gtctgcccca gcttaccggg ctgctgttta atgaccacac accgtttatc
    14401 agtgagcaga cgaaacaatg gctggcagct tcgcccgacg ccaaagttgc accggttgcg
    14461 caaatcggtg aggaatcgaa ggcagccaga gcctgttttg ctgagcaggg tctggaggcg
    14521 gcgctgcgat atctggacat gctacccgaa ggcgatcccc gcgatcagtt tcaccgccag
    14581 taccttgccg cacagttgac ggaagaggcg gggctggtac agcttgcgca gcagcagtac
    14641 cggatgttgt tcaggatggg attgcagatg atggtggcag actgggagcc gtccctgctt
    14701 gagcagctag aacaaaaatt cacggcagaa caataaaaga gaacaggagt ttttgtgttc
    14761 agattaccaa caccccgact actcagcgga ctcaaatcag ccctgcgacc ggcgatgccc
    14821 cggtttaaag tctctgcttt ctggctgctg atactggcgt ggatttttct gctggtgtgg
    14881 atctggtgga aaggcccgat gtggacgctg tatgaagaac agtggctcaa accactggcg
    14941 aaccgctggc tggcaacggc ggcgtggggg attattgccc tggtgtggct taccgtccgg
    15001 gtgatgaagc gcctgcaaca gcttgaaaaa atgcaaaagc aacagcgcga ggaagccgtt
    15061 gatccgctca gcgtggaact gaacgcccag cagcgttatc ttgaccgctg gctgctgcgc
    15121 ctgcaacgcc atctcgacaa ccgccgtttc ctgtggcagt tgccgtggta tatggtcatc
    15181 ggcccggcgg gcagtggcaa aacgacactg ctgcgcgagg ggtttccgtc cgacattatt
    15241 tatgccccgg agggcgcacg cggcgcagaa caacgcctgt acctcacgcc ccatgtcggt
    15301 aaacaggcgg tgatctttga tatcgacggc acactctgcg ctcccgctga tgcggatatc
    15361 ctgcatcgcc gtctgtggga acatgccctc ggctggctga aagaaaagcg cgcgcgccag
    15421 ccgctgaacg ggattattct gacactcgat ttacccgatc tgctgaccgc agacaaacgc
    15481 cgccgcgagc atctgttaca gacgctgcgc agccgcttac aggatatacg ccagcatctt
    15541 cactgccagt taccggttta cgtggtactg acccggcttg atttattaca gggttttgcc
    15601 gccctgttcc agtcactcaa cagacaggat cgcgatgcga ttctgggcgt cacgttcacc
    15661 cgccgtgccc atgaaaatga tgactggcga acagagttga atgctttctg gcagacatgg
    15721 gtggatcgaa tgaatctggc gttgccggat ctgatggtcg ctcagactca cacccgcgcg
    15781 tctttattca gtttttcccg ccagatgcag ggaagccgtg aaccgctggt gtcactgctt
    15841 gagggtctgc ttgatggcga aaatatgaac gtgatgctgc gtggtgtcta tctcacctct
    15901 tcgcttcagc gtggacagat ggatgatata ttcacccagt ctgccgcccg ccagtaccgg
    15961 ctgggcaata acccactggc gtcctggccc ctggtggaca ccgcgcctta tttcacccgc
    16021 agcctgttcc cgcaggcatt actcgcagag cctaatctgg caacagagag ccgcgcctgg
    16081 ctgatacgtt cccgtcgccg cctgacggtt ttctctgcca caggcggcgt ggcagcactg
    16141 ctgctcatca ccggctggca tcactattac aacggtaact atcagtccgg catcaccgtg
    16201 cttaagcagg ccaaagcctt tatggacgtg ccgcctccgc agggggaaga tgactttggc
    16261 aacctgcaac tgccgctcct gaacccggta cgcgatgcca cactggccta tggcgactgg
    16321 ggcgaccgca gccgtctggc cgatatggga ctgtaccagg gacgacgtat cgggccttat
    16381 g
//
LOCUS       DAZOZD010000079        16373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOZD010000079 DAZOZD010000000
VERSION     DAZOZD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.079"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            86..1111
                     /gene="dgcP"
                     /locus_tag="ACHUPB_002067"
     CDS             86..1111
                     /gene="dgcP"
                     /locus_tag="ACHUPB_002067"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0758880.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            1144..1242
                     /locus_tag="ACHUPB_002068"
     CDS             1144..1242
                     /locus_tag="ACHUPB_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0758881.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            1245..1319
                     /gene="yoaJ"
                     /locus_tag="ACHUPB_002069"
     CDS             1245..1319
                     /gene="yoaJ"
                     /locus_tag="ACHUPB_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0758882.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(1378..1626)
                     /locus_tag="ACHUPB_002070"
     CDS             complement(1378..1626)
                     /locus_tag="ACHUPB_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0758883.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1774..1956)
                     /locus_tag="ACHUPB_002071"
     CDS             complement(1774..1956)
                     /locus_tag="ACHUPB_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR0758884.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(1960..2319)
                     /gene="yeaR"
                     /locus_tag="ACHUPB_002072"
     CDS             complement(1960..2319)
                     /gene="yeaR"
                     /locus_tag="ACHUPB_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR0758885.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(2492..3130)
                     /gene="leuE"
                     /locus_tag="ACHUPB_002073"
     CDS             complement(2492..3130)
                     /gene="leuE"
                     /locus_tag="ACHUPB_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR0758886.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(3257..4180)
                     /gene="dmlR"
                     /locus_tag="ACHUPB_002074"
     CDS             complement(3257..4180)
                     /gene="dmlR"
                     /locus_tag="ACHUPB_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR0758887.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            4283..5368
                     /gene="dmlA"
                     /locus_tag="ACHUPB_002075"
     CDS             4283..5368
                     /gene="dmlA"
                     /locus_tag="ACHUPB_002075"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR0758888.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            5619..7229
                     /gene="yeaV"
                     /locus_tag="ACHUPB_002076"
     CDS             5619..7229
                     /gene="yeaV"
                     /locus_tag="ACHUPB_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR0758889.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            7261..8385
                     /gene="yeaW"
                     /locus_tag="ACHUPB_002077"
     CDS             7261..8385
                     /gene="yeaW"
                     /locus_tag="ACHUPB_002077"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR0758890.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            8441..9406
                     /gene="yeaX"
                     /locus_tag="ACHUPB_002078"
     CDS             8441..9406
                     /gene="yeaX"
                     /locus_tag="ACHUPB_002078"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0758891.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(9460..10575)
                     /gene="rnd"
                     /locus_tag="ACHUPB_002079"
     CDS             complement(9460..10575)
                     /gene="rnd"
                     /locus_tag="ACHUPB_002079"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0758892.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10657..12342)
                     /gene="fadD"
                     /locus_tag="ACHUPB_002080"
     CDS             complement(10657..12342)
                     /gene="fadD"
                     /locus_tag="ACHUPB_002080"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0758893.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(12547..13128)
                     /gene="yeaY"
                     /locus_tag="ACHUPB_002081"
     CDS             complement(12547..13128)
                     /gene="yeaY"
                     /locus_tag="ACHUPB_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0758894.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(13168..13863)
                     /gene="tsaB"
                     /locus_tag="ACHUPB_002082"
     CDS             complement(13168..13863)
                     /gene="tsaB"
                     /locus_tag="ACHUPB_002082"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0758895.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13921..15831)
                     /locus_tag="ACHUPB_002083"
     CDS             complement(13921..15831)
                     /locus_tag="ACHUPB_002083"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR0758896.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15963..16307
                     /locus_tag="ACHUPB_002084"
     CDS             15963..16307
                     /locus_tag="ACHUPB_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0758897.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4019 a         4262 c         4012 g         4080 t
ORIGIN      
        1 ttgtttcatc tttgttggtg atgttttatc atgcctgcaa agattaaata atcagcattt
       61 acccgccgta tcctggagtt gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc
      121 ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga tgctggaaat
      181 ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc gcctgcagca
      241 tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta ccgtctcgtg
      301 ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg atgaagtccc
      361 tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atctccacat ttctgagcac
      421 accaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca gcagtgagaa
      481 gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac tgattgcaca
      541 atatattcaa aaagaggcac tggttgaaca gttgcgcgaa gccaatgctg cgctgattgc
      601 gcaatcgtat accgactcgt taaccgggtt gccgaatccg cgggcgattt ttgaaaatct
      661 gacgacgctg ttttctctcg cccggcatct taaccataag ataatgatcg cgtttatcga
      721 tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg atctgtttct
      781 cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct
      841 cggcggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata tttcgtcgct
      901 gcgagaacgc attcagcagc aaatacgtgg agaatatcac ttaggtgatg ttgatttgta
      961 ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg atgcagacag
     1021 tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc
     1081 ttttgtcgcg cattcagcgc cacattgctg aggcgtattc acatcctttt gattggtgat
     1141 aacatgcgaa tcggtattat ttttccggtt gtaatcttta ttacagcggt cgtattttta
     1201 gcatggtttt ttattggcgg ctatgctgcc ccgggggcat aaagatgaaa aaaacaacga
     1261 ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga tggtggtaac
     1321 gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta
     1381 acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg caaccacgaa
     1441 gctgccaaaa ttgaagccat cgactttacc aaagccaaac agcgtgctga tccatccgcc
     1501 gactacggca ccgactatcc ccagcaggat agtcataaag aatccaccac catctttacc
     1561 tggcatgatc cacttcgcca gaataccggc aataagccca aaaataatcc atgacagaat
     1621 gcccattgtt tcctcactta tctgttttgc attagcgggt tagtcgctga taaaaagcat
     1681 agcacaacat cgggagggca agatttgtga cgagcatcac ggaggatttt tttgcgatgg
     1741 cgcagaaatt gcgccatcaa cgatcagtga taattaccaa ccacaaacat catgttcgtt
     1801 ttccgtgtca taagaacgca cggtattcac cagatctttt atcacttcag ccgccacttc
     1861 tggcaccagc aaagtcatcg gcgtctctgt ttcataatcg acagaaacgc cattactgtt
     1921 attggtgacg gtcacggtat acgttgcttt gcccatgatt catttcccgt tatgaatgac
     1981 tttccgttgt tgcgcacctt ccatcaggac ttcaggagcc acgaagaagt caatgttgaa
     2041 ataagtatcg tcagtcatgg cttcaatgtt gtgccacttt tctggaggga acacctcaaa
     2101 ctgccccgct tcgataagga tcacctgatc aggctctgca ctgtgttcat cagcgtagcc
     2161 gagatatttg accgccccat gcataacgga aaggcgtggg taaacccccg ggcgcgttcc
     2221 tttatcaaga tgacgttcga atattccggc aggcgcagtt tgtttattcc agaaaggcgt
     2281 tgagcgcgta tgaatataat tctgtgggat ttgcagcatc cttttccctc cttcggtgaa
     2341 tgcgctgaaa acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa
     2401 taccatttat tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt
     2461 gggaagcccg cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg
     2521 cgaaacccac gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg
     2581 tacgtatgta ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac
     2641 tcaccagttc cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa
     2701 catcgataaa ctgtacgaaa aacgacacat agaacaaaat ggctttcgga ttagtcaggc
     2761 tcaaaattaa cgcgcgttta aaaatggccc cgtattgggg ctcatcggat ttggcctcgt
     2821 tatttttacc cttcagggtc gcgtagagaa ttttactccc cagatagagc aaataaaacg
     2881 caccaagata acgtacaatg ttgaataata tcggggtggt cttaattaat gtcgccactc
     2941 cagcccatgc cagaaacatc aatactgcat cgccaataaa tacaccgcag gccgcaagat
     3001 aaccgccttt cataccgcta ctgacgctat ttttgagtac aaacagggta tttggccctg
     3061 gcaccaacac aataaaaatg gccccaacca gataggtcca gtaattcaga accccgtatt
     3121 cagcgaacac attaacctct ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg
     3181 ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt tttaacccga tatcaacccg
     3241 ataattgaat aattaatcag gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat
     3301 actggcgtaa aaattctatc gttatccgca ctttcgctga cgtcgccagc cttgaaacat
     3361 aaacggccca gacgttcgct ggctggtaat attccggtag cacttgcact aaatgaccac
     3421 tggcaatgtt ttcgctaaca tcccaccagg agcgcagagc aatcccctgc ccatcgagac
     3481 accactgatg gacaatttct ccatgattag acgacagtgg gccggtgact ttaatcgcat
     3541 ggtggccttc tttattgcgt aattgccaga caccaaaagg atgatcgcgc tctttaatga
     3601 ccaggcacgg caatgcggaa agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa
     3661 attccggcga cgcgcagaga atgcggtaat tagtcgccag tttgcgggcg attaaattag
     3721 gggcgatgtc atcgccaatg cgaatatcga gatcaacacc ttcattgacc aaatcgacca
     3781 accggtcttc cacatcaaaa cgtaattcca gttgcggata cgccttagcc agcgctgata
     3841 acgccggagc caccacctgt cgtccaaaac caaagctgct gataatacgc agcatcccct
     3901 gcggcacctg acgcacgtca gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc
     3961 gctgcgccca ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc
     4021 ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg
     4081 ccggtgaaac gcccagttct tccgccacgg cggcaaaacc agcccggcga gccaccagca
     4141 taaagacgcg caaatcgttc agcagcggta aattattcat gattcgtgtt ttatgtttca
     4201 ccagttacgg ggattaattc cttttcagtc aattatagga tggtgatgtt gtcaattttg
     4261 atggtcagga agtgagaacc caatgatgaa aacgatgcgt attgctgcga tcccgggaga
     4321 tgggattggc aaagaagtcc ttcctgaagg gattcgcgta ttacaggctg ccgctgagcg
     4381 ctggggcttc gacttgagtt ttgagcaaat ggagtgggcg agctgcgagt attacagcca
     4441 tcacggtaaa atgatgccgg acgactggca tgagcaactt agccgtttcg acgccatcta
     4501 ttttggtgcc gtcggctggc cggataccgt tccggaccat atttcgttgt ggggttcgct
     4561 gctgaaattt cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc gtctctttcc
     4621 tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac atcgattttt acgtggtcag
     4681 ggaaaacacc gaaggcgaat attcctcgct cggcggtaga gtgaatgaag gtacagagca
     4741 tgaagtcgcc attcaggaat cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta
     4801 tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta acttctgcca ctaaatcaaa
     4861 cggtttagcc atcagcatgc cgtactggga tgagcgagtg gaagcaatgg ccgagaatta
     4921 cccggagatc cgctgggaca agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca
     4981 gccggaacgc ttcgatgtgg tggtggcgtc caatttgttt ggcgatatcc tttccgatct
     5041 tggcccggcc tgcaccggca ccattggcat tgccccatcc gccaacctga atccggaacg
     5101 cactttcccg tcgctcttcg agcctgtcca cggttccgcg ccggatatct acgggaaaaa
     5161 tattgctaac cctatcgcca caatttgggc cggtgcgatg atgctcgatt ttctcggcaa
     5221 tggcgatgag cgtttccagc aagcgcataa cggtattctg gcagcgattg aagaagtgat
     5281 tgctcacggg ccgaaaacac cggatatgaa aggcagtgcc accacgccac aggttgccga
     5341 cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg
     5401 acgcggtttt tttattcgtt ctttgcagtt aataacctgc gtcatttcac cttttattgt
     5461 ttccgtttcg tgttttatgg ctttccgtat tcttaattgt ttaatttatg taacatgcaa
     5521 ataatcaatt tgttaaacac aacatacaac tcattaccct gcaaataatt acaactggca
     5581 aaagccagag acgggctttt atgcccagga gatgactcat gatgagcaat gttaagaaaa
     5641 aagatgtgcc gctgataagc atcagcctgg tggccattct tttcatcgca gctgcattaa
     5701 gccttttccc acaacaatcg gccgacgcgg ccaacacaat atacactttt gttacgcgta
     5761 cgttaggttc cgccgtacag gtattggttt tgctggcaat gggactggtg atttatttag
     5821 ccaccagtaa atacggcaat attcgtcttg gcgaaggaaa accggaatac agcacgctct
     5881 cctggctgtt tatgtttatt tgtgccggtt taggttcttc tacgctttat tgggggggtg
     5941 ctgaatgggc ctattattat caaacgcctg gattaaatat cgcaccacgt tcacaacagg
     6001 cactcgaatt tagcgtccct tactccttct tccactgggg catcagcacc tgggcaactt
     6061 atacgctggc ctcattaatc atggcttatc actttcatgt gcgtaaaaac aaaggtctga
     6121 gtctttccgg cattattgcc gcgattaccg gcgttcgccc gcaaggccca tggggaaaac
     6181 tggttgattt gatgtttctg atcgccactg tcggcgcact gactatttcc cttgttgtca
     6241 ccgcagcaac ctttactcgt ggactttccg cgctgaccgg tttacccgat aacttcaccg
     6301 tgcaggcatt tgtgatcctg ctttccggcg gcattttttg cctaagctcg tggattggta
     6361 tcaacaacgg tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat
     6421 tactggtgct gattgtcggt ccaaccgaat ttattaccaa cagcatcatc aatgccatcg
     6481 gcctgaccac gcaaaacttc ctgcaaatga gcttattcac cgatccgctt ggcgatggtt
     6541 catttacccg caactggacc gttttctact ggctgtggtg gatctcatac ccccctggcg
     6601 tagcaatgtt tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt atctggggac
     6661 tgatcctcgg cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa agctatgcca
     6721 ttcatcagtt tatcaatggc gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg
     6781 aaacagctgt acagcaagtt ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat
     6841 acctgggcgt gatgattatt ttccttgcct cgcatatgga tgcagtggcc tacaccatgg
     6901 cggcgaccag tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt
     6961 tctggtgcgt ggtgatcact ctgatcccgc tttccatctt gtttaccggt gcttcgctgg
     7021 aaacgatgaa aaccaccgtc gtgctcacag cccttccctt cctcgtcatt ttactggtga
     7081 aagtcggcgg atttattcgc tggctgaaac aggattatgc cgacattccg gctcatcaag
     7141 ttgaacatta tctcccgcag acaccggttg aagccctgga aaaaacacca gtgctccctg
     7201 cgggaaccgt attcaaaggc gacaactgag cgccatcatc ctaacgataa aggtatccct
     7261 atgagcaatc tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag
     7321 gcgtggacca ttcctgcccg tttttatacc gatcagaacg cgtttgaaca cgaaaaagag
     7381 aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca gcgaactggc gaatgccaat
     7441 gattatgtga cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag
     7501 gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa
     7561 ggaaaagcaa aaaatgtgat tacctgccca tatcacgcat gggcattcaa actcgatggc
     7621 aatctggccc atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa
     7681 ctggttccgg tgcgtctgga ggaatatgcc ggattcgtct tcatcaacat ggaccccaac
     7741 gccaccagcg tagaagatca gttacccggt ctgggcgcga aagtgctgga agcctgcccg
     7801 gaagtccacg atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag
     7861 aacattgtcg ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc
     7921 gactccgtac aggttgatcg ctactggcac accatgcacg gtaactggac gctgcaatac
     7981 ggtttcgcca aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc
     8041 cacggtttct ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg
     8101 acggtcattt atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac
     8161 ttcaccaatg aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg
     8221 ttccgtccgg aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc
     8281 tatcgtggtc aggggcgcat catggccgac agtagcggta gtggcatttc cgaacatggt
     8341 atcgcccatt tccataatct gctggcgcag gtgtttaagg actaatgaca tcggcggcgg
     8401 tattttccgc cgctgggctg atttttgatg gagtacagca atgtcagact atcaaatgtt
     8461 tgaagtacag gtgagtcagg tggaaccgct taccgaacag gtgaaacgct tcacgctggt
     8521 ggcaaccgat ggcaaaccgc tgcctgcgtt caccggagga agtcacatca ttgtgcagat
     8581 gagcgatggt gataaccagt acagcaatgc gtattcacta ctgagttcgc cgcatgacac
     8641 ctcttgttat cagattgccg ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt
     8701 gcatcagcag gtaaaagtgg gcgatcggtt aacgatttca acgcctaata acctgtttgc
     8761 gctaattccc tcagccagaa agcatctgtt tatcgcgggc ggtattggta tcaccccttt
     8821 cctgtcgcac atggcagagt tgcaacacag cgacgtcgac tggcagctac attactgctc
     8881 gcgaaatcca gaaagttgcg catttcgtga tgagctagtc cagcatccgc aggctgagaa
     8941 agtccatttg catcattcgt caaccggaac acgactggaa ttagcgcgat tattggcgga
     9001 tatcgaacct ggcacacacg tttatacctg tggccccgag gcgctaaatg aagcggtaag
     9061 aagtgaagct gcgcgtctgg acatcgccgc cgatacgctg cactttgagc aatttgctat
     9121 cgaagacaaa accggcgatg cctttaccct ggtgcttgcc cgttccggaa aagagtttgt
     9181 ggtgccggaa gagatgacta ttttgcaggt tattgaaaat aataaagccg cgaaagtgga
     9241 atgtttatgt cgtgaagggg tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc
     9301 tgaccatcgg gatcagtatt ttagcgatga agagcgtgcc agccagcaaa gtatgttgat
     9361 ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg
     9421 atgtggcgct gagcgcgccc agtccggctt cggaagattt tactgcggat attcctgcaa
     9481 taaattgtgt aatgcttccg ccatcagctc accacgccag ccggaaatca gctccggcaa
     9541 attgttctgc ggtttcagtt tccagtgcca gttcagcagt tggttgattt gccgacgcga
     9601 tgccagcaat tcggcgctga tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat
     9661 cgctttaaac gctttacgat aacccggcat gtccatcagg ttaagcatcg gctgcggtaa
     9721 ggcctcttcc ggcaatgtct gcgctttttc caccagcgcc agcagcgttt taccgtgaaa
     9781 gcggatttcg ctaccggata aacccaggct gtccagttcg cctaaacttc ccggcatata
     9841 acgcgcgacc gaccacaaat gctcttcacg cacgacaaag ttcaccgcca gatcacgctc
     9901 acgagccttg cgcagtcgcc agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg
     9961 taattgccag gcattggtga tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg
    10021 acgcatttgc atcaggcggc attcatccag cgccgcaggt agccagccgg aggcctccgt
    10081 ttctaccata agcttggcgg tgatcggtaa cagataccag acatccgccg ctgcgtattc
    10141 acactgacgt tcggtcagcg gtctggccag ccagtcggtg cgcgattcac tcttgtccag
    10201 cgtaacgccg gaatactctt ccaccatgga agcgaaaccc catgacatcg ggcgtccgca
    10261 gaaggcagca aggatttgcg tgtcaatcag gggttgtggt aattcgccaa agacattgag
    10321 gaacacttcc agatcttcac tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag
    10381 gatcgctttc agcggtgacc agtcggtgat cccgagagga tcaattagcg ccagatgctc
    10441 accatcgaaa agttgaatca accccagctg cgggtaataa gtgcgcgtac gaacaaattc
    10501 agtatcctgg gctatcgccg gaaagacacg gacggcttca cacaaagaag ccagcgcatc
    10561 gtccgtggta atcatttggt aattcaaatt gtattctctt tagtgggcgt caaaaaaaac
    10621 gccggattaa ccggcgtctg acgactgact taacgctcag gctttattgt ccactttgcc
    10681 gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc
    10741 atcacgaaac tccaccagct tcggtacttt gtagcccgtg agctgacggc agcaaaaggt
    10801 caccagtgac tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc
    10861 accactggag ccggaaggta cgccaacagc cgcgacttcc tgtacgccag gatgctgcat
    10921 gacgacatct tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt
    10981 tttacgatcg acaatgcgca ggaacccttc ttcatccatc accgcgatgt cgccggtgtg
    11041 taaccagcca tttttgatga tctcatcggt agcatcggga cgctgccagt aacccagcat
    11101 cacctgcggt cctttgacac aaagctcacc cggttgaccc ggtggtactt cattatcgtc
    11161 gtcatccacc agtttggctt ccgtcgacgg caccggcaaa ccgatgctac cactatgata
    11221 atcaatatca tatgggttaa cgctgaccag cggcgcacac tcggtaagac catagccttc
    11281 cagcagatac tgtcctgtca gtttcaccca acgctctgcc accacttgct gcactggcat
    11341 cccaccgcct gcggaaagat gcagactgga gaaatccagc tgctggaact ctttattgtt
    11401 cagcaacgca ttgaacaagg tgttaacgcc agtgatagcg gtaaacggat atttcgctaa
    11461 ctcttttacc aaccctggaa tatcgcgcgg gttagtgata agcaggttct gcccacccag
    11521 ttcgataaac agcaggcagt taatggtcag ggcaaaaatg tgatacagcg gcagtgccgt
    11581 tactaccagt tctttacctg gatgcagcaa cggcccatag gtggcgttga cctgttccag
    11641 gttcgccagc atattgcggt gagtcagcat cgcgcctttc gccacacctg tggtgccgcc
    11701 ggtgtattgc aggaaggcta aatcttccgg caccagttcg ggtttgacgt actgcatccg
    11761 gtagccgtta tgcagtgcgc tacgaaatga aatggcatct ggcagatggt atttcggcac
    11821 caaacgcttg atgtatttaa caacgaaatt gactaccgtg ccttttgccg tagatagctg
    11881 atcgcccata cgggtcagaa ttacgtgctg aacggcggtt ttatcaacca ctttttccag
    11941 tgtgtgagca aagttagaca cgataacaat cgccgatgcg ccgctatcgt taagctgatg
    12001 ctcaagctca cgcggggtat acaacgggtt aacgtttacg acgatcatcc cggcacgcaa
    12061 aatgccaaac agcgccaccg gatattgcaa taaattaggc atcatcaacg caacgcgatc
    12121 gcctttcttc agccccaacc cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc
    12181 cagcttgcgg aaggtcatta cctcccccat attcacaaac gcaggctgat cggcgtagcg
    12241 gacgaccgac tgctcaaaca tatctatcag agattgataa cggtcagggt tgatctccgt
    12301 cggaacatcc gcgggataac ggttaagcca aaccttcttc aattcttcac ctctaaaatg
    12361 cgtgttcgtc gtcatcgcaa ccccaagtga taaacatgtt gttaacataa cattaactca
    12421 tcttaccagc ttgataatta gacaacgaaa aggttgcgaa gcgcgtcact atttattttt
    12481 atagttgccg tatgaacgca gaaacagcgg accagccgct gtttcttttt ctttaaaaac
    12541 aacttgttac tcggttacaa ttgtttcaac tcttgcaggg ccaggattat accaacccca
    12601 tccgccgtaa ccatagggcc agccgcgtcc accataaaac catggatcaa ttggctgcgg
    12661 cggcataatc acctgttgag tgatatgcca gcgtttgtaa cctgttacct gcataaccat
    12721 aaatttatat ggtgcattat ttactttgcc atccactgtg ccagtgattg gcccgacaac
    12781 cgtcaccagt tgtccacgaa aatcgaccgg atcaagaaaa ccgttcacat cggcataaat
    12841 gcgcccgcgt gaaggttctc ccaaggttgg tcttgctccg ctgtccagcg gtacggtagc
    12901 aatttccagg cgggttttcc cttgctggtt ttgtaccaca accactttgc cgccaaagcg
    12961 cgcctcctga ccaacatata aatctggcgc attcatcacg cgtaccagat cttgctgtgg
    13021 tgttggactg ctgcctttaa tggcgtccgg cacagtgaca caaccgctca gcattagcgc
    13081 aaacgtacct gccagtatgc ctttgataac attcttttga accgccatga tgcgactcct
    13141 ttttttctca gggcatactc ttaagattca ttctttgccc ggaagtttct tccatgcgac
    13201 gttgttacgt aaataaaccg gttcggcatg ttccaccgcc accgttttac cctcggcaaa
    13261 catctgacac gcaatcggca gcatatcttc cgccgctggc agtaaaactt ctccgtcgcg
    13321 caaaaccagc ccgctctctt tacggagatc cggccaggct tgccagcccg tccctaccgt
    13381 tacccattcg ccggaaagct gttgcattcg ttcatgaacg agttcgggtt tgagtacggc
    13441 ttcggtttct tcaccgcgcc agataccgtt ttcatcacgc tgatattcgg cccagtaaac
    13501 ttcgcccatt cgcgcgtcaa tggctgccag cacgcgggtt gcgccgtttt tgcgccacgc
    13561 cccctgcgcc atcgtcatta gcgtagagac accaatcatc ggtaactccg cgccaagcgc
    13621 cagcccttgc gcaataccaa taccaatgcg cacgccagta aagctaccgg ggccgcgccc
    13681 gtaagccaga gcgtttatgt cagtcaggga agttccgctg gtggtcagga tatcctgcac
    13741 catcggtaag attcgttgag tatgttcacg agggcaaagc tcaaaatgag cgttgacatt
    13801 accgtcgttc cacagagcga cagagcagac ctctgtcgcg gtatcgatag ccagaattcg
    13861 catgggtctt cgtgcttaga tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa
    13921 ttacctggag gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac gggtgcgtgg
    13981 cgcgggcggc agactggcga gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc
    14041 acaaattacc agcacgccgc gatcgtcggc gtcgcgaatc agtcgcccta ctccctgctt
    14101 gagagtaatg accgcatccg gcagctgcac ttcatcgaac gggtcgccac cgcgcaaacg
    14161 acaatcttcc atgcgcgctt ttaacagtag atcatccggc gaggtaaacg gcaatttgtc
    14221 gataattacc aatgacaatg tatcgccacg cacgtccacc ccttcccaga aactactggt
    14281 tgccacaaga agcgcgttac cggcgctgac aaattgctgc aacagttgcc ctttgctggt
    14341 ttccccctgc aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg
    14401 catcatggca tgcgaggtac aaagcataaa acaacgaccg ttgttagctt cgatgatcgg
    14461 tcgcagcatt gccgccagtt gccgtgcaca acccggttga ttggtttgcg gcaggttgcg
    14521 cggcacacag agtaacgcct ggcggctgta atcaaacggg cttggcaata gcaacgactc
    14581 cgcctgttct atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt
    14641 tgctgaggtg aagatccagc taccgggctt ttgcgccatt aactctttga atttatccgc
    14701 cacgctgagc ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt accagtagct
    14761 gtagcccggc tgattgatct cttttagccg cttcagccgt gtgcgataca acgtggcgcg
    14821 ctcaaatgcc gcatccagca aggcggaacg ccccagtgac agtttcgcca cgtcataaca
    14881 aagttccagg gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat tagctaacag
    14941 ctcacgcagg ttaccgcgat aacctggctc accgagttgc agacgaaaat cctgtgcact
    15001 ctgggcaaga cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt cggtgcggta
    15061 ggcgatggtg atgtcttttg ccaggtcgag cagttgtcga ctggagagtg actgaccaaa
    15121 atactggctg gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc
    15181 agggatcaac tcgccaaatc cactctcttt aaccaccata tccgccagaa agagatgatg
    15241 gtttaccacc accacatcgg cgtccatcgc ttttttacgt gctttgacca caaagcaatc
    15301 tttatacatc gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga ccagcggcca
    15361 cgcctgtgaa tcttccgcca cgctgacgca ggtgctgata tcaccatcga ctgtttgatt
    15421 agaccaggag cgcagcagga tcacatcgct taagatttgt accggcagat cgccccccgc
    15481 cagcgcctgc tgttcgagac gttcgaggca gaggtagttt gagcgccctt tcagcagcgc
    15541 cacgttgccc gtatatttca atgcctttga gactgttggc aaatcgcggc tgtagagctg
    15601 atcctgcaac gcttttgagc cggtcgagat aatgactttc tttttcgccc gcagcgcagg
    15661 agccaggtaa gcgtaggttt tgcccgtacc ggttcctgct tccaccacca gcggctggcc
    15721 tttttctatc gcctgggtga cggctaccgc catctgtcgc tgtggttctc gcggcttaaa
    15781 gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc
    15841 tgttgatttg aacagggatt atgtcaggat gagggcgctt tcgccagttg aagtggtgac
    15901 ggcaacctca cattgtggca gtctttgcag cacgaaaatg gaagtataat gaggacaaaa
    15961 tgatgactat cgttcgtatc gatgctgaag cccgctggtc tgatgtagta atccacaaca
    16021 acacgctcta ctacactggt gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg
    16081 ccaacacgct ggcacagatt gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca
    16141 ttctggatgc caccattttc ctggccgata aaaacgactt cgcggcgatg aataaagcgt
    16201 gggatgcctg ggttgtcgcg ggtcatgcgc cagtgcgctg cacggtacaa gcgggtttga
    16261 tgaacccgaa gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc
    16321 aaaataagtc agacgataat ttatcgataa tactggtcgg ttttacataa atc
//
LOCUS       DAZOZD010000080        16296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOZD010000080 DAZOZD010000000
VERSION     DAZOZD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.080"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..578
                     /locus_tag="ACHUPB_002085"
     CDS             <1..578
                     /locus_tag="ACHUPB_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0758898.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="ACHUPB_002086"
     CDS             581..1744
                     /locus_tag="ACHUPB_002086"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0758899.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="ACHUPB_002087"
     CDS             1828..3453
                     /locus_tag="ACHUPB_002087"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0758900.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUPB_002088"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUPB_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0758901.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUPB_002089"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUPB_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0758902.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUPB_002090"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUPB_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0758903.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUPB_002091"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUPB_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0758904.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUPB_002092"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUPB_002092"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0758905.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUPB_002093"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUPB_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUPB_002094"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUPB_002094"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0758906.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="ACHUPB_002095"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="ACHUPB_002095"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0758907.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="ACHUPB_002096"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="ACHUPB_002096"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0758908.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="ACHUPB_002097"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="ACHUPB_002097"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0758909.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="ACHUPB_002098"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="ACHUPB_002098"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0758910.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="ACHUPB_002099"
     CDS             12037..12588
                     /locus_tag="ACHUPB_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR0758911.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="ACHUPB_002100"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="ACHUPB_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="ACHUPB_002101"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="ACHUPB_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0758912.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="ACHUPB_002102"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="ACHUPB_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0758913.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="ACHUPB_002103"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="ACHUPB_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0758914.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="ACHUPB_002104"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="ACHUPB_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0758915.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="ACHUPB_002105"
     CDS             complement(14856..15827)
                     /locus_tag="ACHUPB_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0758916.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..>16296)
                     /locus_tag="ACHUPB_002106"
     CDS             complement(15880..>16296)
                     /locus_tag="ACHUPB_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR0758917.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4155 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatga
//
LOCUS       DAZOZD010000081        16181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOZD010000081 DAZOZD010000000
VERSION     DAZOZD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.081"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUPB_002107"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHUPB_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0758918.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="ACHUPB_002108"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="ACHUPB_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0758919.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="ACHUPB_002109"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="ACHUPB_002109"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0758920.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="ACHUPB_002110"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="ACHUPB_002110"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0758921.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="ACHUPB_002111"
     CDS             complement(5254..6294)
                     /locus_tag="ACHUPB_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0758922.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="ACHUPB_002112"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="ACHUPB_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0758923.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUPB_002113"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHUPB_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0758924.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="ACHUPB_002114"
     CDS             7229..7669
                     /locus_tag="ACHUPB_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0758925.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUPB_002115"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHUPB_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0758926.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUPB_002116"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHUPB_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0758927.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..10832
                     /gene="rsxC"
                     /locus_tag="ACHUPB_002117"
     CDS             8898..10832
                     /gene="rsxC"
                     /locus_tag="ACHUPB_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0758928.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10833..11891
                     /gene="rsxD"
                     /locus_tag="ACHUPB_002118"
     CDS             10833..11891
                     /gene="rsxD"
                     /locus_tag="ACHUPB_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0758929.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11895..12515
                     /gene="rsxG"
                     /locus_tag="ACHUPB_002119"
     CDS             11895..12515
                     /gene="rsxG"
                     /locus_tag="ACHUPB_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0758930.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12519..13214
                     /gene="rsxE"
                     /locus_tag="ACHUPB_002120"
     CDS             12519..13214
                     /gene="rsxE"
                     /locus_tag="ACHUPB_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0758931.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13214..13849
                     /gene="nth"
                     /locus_tag="ACHUPB_002121"
     CDS             13214..13849
                     /gene="nth"
                     /locus_tag="ACHUPB_002121"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0758932.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14460..15962
                     /gene="dtpA"
                     /locus_tag="ACHUPB_002122"
     CDS             14460..15962
                     /gene="dtpA"
                     /locus_tag="ACHUPB_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0758933.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16067..>16181
                     /locus_tag="ACHUPB_002123"
     CDS             16067..>16181
                     /locus_tag="ACHUPB_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0758934.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3762 a         4116 c         4124 g         4179 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacagac taacgcagaa ccagaagaac
    10741 aggttgatcc gcgcaaagcg gcagttgccg cggctattgc ccgcgctcag gccaaaaaag
    10801 ccgcccagca gaaggttgta aacgaggact aaatggtatt cagaatagct agctcccctt
    10861 atacccataa ccagcgccag acatcgcgca ttatgctgtt ggtgttgctc gcagccgtgc
    10921 caggaatcgc agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg
    10981 catcggttag tactctgtta gccgaagctc tcgtactcaa actacgcaag cagtcggtag
    11041 ccgcaacgtt gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc
    11101 cccccctcgc gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta
    11161 aacagttgta tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg
    11221 tcttactgat ctccttcccg gtgcagatga ccagctggtt accgccacat gaaattgcgg
    11281 tcaacatccc tggttttatc gacgccatac aggttatttt cagcgggcat accgccagtg
    11341 gtggtgatat gaacacacta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg
    11401 atacatttaa aacctctgtc cgtgccggtc attcggttga acagattatg caatatccga
    11461 tctacagcgg tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg
    11521 gcggcctgtg gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag
    11581 taacgctggc gttatgcgca acgttgggct ggttgttctc accagaaaca ctggcagcac
    11641 cgcaaattca tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc
    11701 cggttaccgc ttctacgacc aatcgtggtc gtcttatttt cggcgcgctt gcgggcttat
    11761 tagtctggtt gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc
    11821 tggcgaacat cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc
    11881 gcaaagggta aaccatgctg aaaactatcc gaaaacacgg cattacgtta gcgctatttg
    11941 cagcgggttc aacagggtta actgcggcca tcaaccagat gaccaaaacg acgattgctg
    12001 aacaggccag tctgcaacaa aaggcattat ttgatcaggt gctgccagcc gaacgctata
    12061 acaatacgct ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc
    12121 atcgggttta catcgccaaa caggatgaca aaccggtagc cgccgttctg gaagcaaccg
    12181 cgccagatgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
    12241 tacttggcac gcgcgtgaca gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
    12301 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
    12361 cgcactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
    12421 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
    12481 cacaactttc tcaacttcct gcctgtggag aataaagcgt gagcgaaatt aaagacgtta
    12541 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtcctc
    12601 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
    12661 tactgacgct gaccaacctg accatttcga cgctgcgtca ctggacgcca gccgagatcc
    12721 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
    12781 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
    12841 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
    12901 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
    12961 tacgcgaaat tatcggcaat ggcacattgt ttgacggtgc agatgcgctg ttaggtagct
    13021 gggcaaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
    13081 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg
    13141 aaagaatgaa aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa
    13201 cagggaatgt ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa
    13261 caatcctcat cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt
    13321 actgctttcc gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactttaccc
    13381 ggtggcgaat acgcctgcag cgatgcttga actgggcgtt gaaggggtta aaacctatat
    13441 caaaacgatt gggctttata acagcaaagc agaaaatatc atcaaaacct gccgtatctt
    13501 actggagcag cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcccgg
    13561 cgtaggtcgt aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc
    13621 tgtcgacacg cacattttcc gcgtttgtaa tcgtactcaa tttgcgccgg ggaaaaacgt
    13681 cgaacaggta gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca
    13741 ccattggttg atcctgcacg ggcgttatac ctgcattgcc cgcaagcccc gctgtggctc
    13801 ttgtattatt gaagatcttt gtgaatacaa agagaaagtt gacatctgaa gaaaaggggt
    13861 aacaccgatt accccattga taacctttct ttatcctctt ttaaaacatc tttttaacgt
    13921 gaatgatgcc attgcttagc gttatcatca ggtaatccgt ttgcggataa ccaaaaatgc
    13981 aggttaattg tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc
    14041 tgtcgcaatg taaaaacatt gcaagaatca ggttaaaata atttaaattt acacacgcaa
    14101 caaatattga cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta
    14161 gtaaaaacgc taaatagcag aacatatcgc cgttaagcca tcacccccct ctcagtgcag
    14221 tgaaaaaatc tgccattacg ttttttgaaa aatttaacgc tggataacat ttcccggaat
    14281 ggttgaattc cccgcctcag ttatatgtaa cagattatta caaaggactt gtctgaaagt
    14341 gcaagatagt gaacattacc tgccgtttcc cctcccacta taacaattgc gcgtatgttt
    14401 tttagacata acgcgagaaa gcacccccgt taatatggga tgtaaaaaaa gaggtaaaag
    14461 tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt gaacgctttc aaacaaccga
    14521 aggcgttcta tctcatcttc tcgattgagt tatgggaacg ttttggttat tacggcctac
    14581 aaggaattat ggctgtttac ctggttaaac aactgggtat gtctgaagcg gattcaatca
    14641 cccttttctc ttcctttagt gccctggttt atggtctggt cgctatcggc ggctggttag
    14701 gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg tgctattgtg ctggcgattg
    14761 gttatgctct ggttgcctgg tctggtcacg acgccggtat cgtttatatg ggtatggcgg
    14821 ctattgcggt cggtaacggc ctgtttaaag ctaacccatc ttctctgctt tctacatgct
    14881 atgagaaaaa cgacccgcgt ctggacggtg cattcaccat gtactacatg tccgtcaaca
    14941 tcggctcttt cttctctatg attgctacgc cgtggctggc cgcgaaatac ggctggagtg
    15001 ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat cgttaacttc gccttctgcc
    15061 aacgctgggt taaacagtac ggttcaaaac cagacttcga gcctatcaac taccgtaacc
    15121 tgctgctgac cattattggt gttgtggcac tgatcgctat cgccacctgg ctgctgcaca
    15181 atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt cggtatcgtg gttatcttcg
    15241 gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa aatgatcgtt gccttcatcc
    15301 tgatgctcga agccattatc ttcttcgtgc tgtacagcca gatgccaacg tcactgaact
    15361 tctttgcgat tcgtaacgtt gagcactcca ttctgggtct ggccgtagaa cctgagcagt
    15421 atcaggcact gaacccgttc tggatcatca tcggtagtcc gattctggcc gctatctata
    15481 acaagatggg cgataccctg ccgatgccaa ccaagtttgc aatcggcatg gtgatgtgtt
    15541 ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc gtctgacgct ggtatcgtgt
    15601 ctgtaagctg gctggtcgca agctatggcc tgcagagcat cggggaactg atgatctctg
    15661 gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg tctgatgggc ttcattatgg
    15721 gtagctggtt cctgaccact gccggtgcaa acctgattgg tggttatgtt gcgggtatga
    15781 tggctgtgcc ggataacgtt accgacccgc tgatgtcact ggaagtttat ggtcgcgtat
    15841 tcttgcagat tggtgtcgct actgccatta ttgcagtact gatgctgctg accgcgccta
    15901 aactgcaccg tatgacgcag gatgacgctg cagacaaagc ggcgaaagct gctgtagcgt
    15961 aaaattcagg gaaactcttt tacaagccgc taacttttcg ttagcggctt ttttttgttc
    16021 agcaacgcac taccatactt taaaccacag acaaaaggag ttaccgatga aattgttcta
    16081 caaaccgggt gcctgctctc tcgcttccca tatcaccctg cgtgagagcg gaaaggattt
    16141 taccctcgtc agtgtggatt taatgaaaaa acgtctcgaa a
//
LOCUS       DAZOZD010000082        16075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOZD010000082 DAZOZD010000000
VERSION     DAZOZD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.082"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            163..1380
                     /gene="alaA"
                     /locus_tag="ACHUPB_002124"
     CDS             163..1380
                     /gene="alaA"
                     /locus_tag="ACHUPB_002124"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0758935.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1464..2063
                     /gene="yfbR"
                     /locus_tag="ACHUPB_002125"
     CDS             1464..2063
                     /gene="yfbR"
                     /locus_tag="ACHUPB_002125"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0758936.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2122..3954)
                     /locus_tag="ACHUPB_002126"
     CDS             complement(2122..3954)
                     /locus_tag="ACHUPB_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0758937.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4041..4689)
                     /gene="hxpA"
                     /locus_tag="ACHUPB_002127"
                     /pseudo
     CDS             complement(4041..4689)
                     /gene="hxpA"
                     /locus_tag="ACHUPB_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4700..5194)
                     /locus_tag="ACHUPB_002128"
     CDS             complement(4700..5194)
                     /locus_tag="ACHUPB_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0758938.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5277..5732)
                     /gene="yfbV"
                     /locus_tag="ACHUPB_002129"
     CDS             complement(5277..5732)
                     /gene="yfbV"
                     /locus_tag="ACHUPB_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0758939.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6070..7272
                     /gene="ackA"
                     /locus_tag="ACHUPB_002130"
     CDS             6070..7272
                     /gene="ackA"
                     /locus_tag="ACHUPB_002130"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0758940.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7347..9491
                     /gene="pta"
                     /locus_tag="ACHUPB_002131"
     CDS             7347..9491
                     /gene="pta"
                     /locus_tag="ACHUPB_002131"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0758941.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9681..11201
                     /gene="yfcC"
                     /locus_tag="ACHUPB_002132"
     CDS             9681..11201
                     /gene="yfcC"
                     /locus_tag="ACHUPB_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0758942.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11234..11776)
                     /gene="yfcD"
                     /locus_tag="ACHUPB_002133"
     CDS             complement(11234..11776)
                     /gene="yfcD"
                     /locus_tag="ACHUPB_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0758943.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11834..12385)
                     /gene="yfcE"
                     /locus_tag="ACHUPB_002134"
     CDS             complement(11834..12385)
                     /gene="yfcE"
                     /locus_tag="ACHUPB_002134"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0758944.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12441..13085)
                     /gene="yfcF"
                     /locus_tag="ACHUPB_002135"
     CDS             complement(12441..13085)
                     /gene="yfcF"
                     /locus_tag="ACHUPB_002135"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0758945.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13221..13868
                     /gene="yfcG"
                     /locus_tag="ACHUPB_002136"
     CDS             13221..13868
                     /gene="yfcG"
                     /locus_tag="ACHUPB_002136"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0758946.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13925..14287
                     /gene="folX"
                     /locus_tag="ACHUPB_002137"
     CDS             13925..14287
                     /gene="folX"
                     /locus_tag="ACHUPB_002137"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0758947.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14308..15201
                     /locus_tag="ACHUPB_002138"
     CDS             14308..15201
                     /locus_tag="ACHUPB_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0758948.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15249..>16075)
                     /gene="rpnB"
                     /locus_tag="ACHUPB_002139"
     CDS             complement(15249..>16075)
                     /gene="rpnB"
                     /locus_tag="ACHUPB_002139"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0758949.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         3992 a         4282 c         3951 g         3850 t
ORIGIN      
        1 cattattttg tcattattaa gttattaaca gcacaatcga gccttcccct ctggcaaaat
       61 cttattctgc agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc
      121 cacggcaggg agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa
      181 tccagcaaat tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa
      241 cgcctggaag aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccattc
      301 ggttttgacg cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa
      361 gggtattgcg attccaaagg gctttactcc gcgcgtaaag ccatcatgca gcactaccag
      421 gctcgtggca tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag
      481 cttatcgttc aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca
      541 ccagattacc cactctggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat
      601 ctttgcgatg aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg
      661 cctcgtacgc gtgggatcgt tattatcaac ccaaataacc caaccggcgc ggtatattcc
      721 aaagagcttt taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc
      781 gatgaaattt atgacaaaat tctctacgac gatgctgagc atcactcaat tgcgccactg
      841 gcacctgacc tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc
      901 ttccgtcagg ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa
      961 ggtctggaaa tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt
     1021 cagaccgcgc tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt
     1081 tatgagcagc gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg
     1141 aaacctcgtg gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac
     1201 gacgatcaga aaatggtgct ggatttcctg ttgcaggaaa aagttctgtt ggtgcaaggg
     1261 acggcattca actggccgtg gccggatcac ttccgcattg tcacgctacc gcgtgtcgat
     1321 gatatcgagc tgtctttgag caagttcgcg cgtttccttt ctggttatca tcagctgtaa
     1381 tcttaatttc actgccggag attgcatccg gcagcgttat cccgccacaa tgacctgatg
     1441 atgtcatcat acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcccgcc
     1501 tgaaactcat taaccgctgg ccgctaatgc gcaacgtgcg gacggaaaat gtgtccgaac
     1561 acagtttgca ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cgaaaatttg
     1621 gcggtaatgt caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag
     1681 tgctcaccgg cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt
     1741 acaaggctat tgaaaaaatc gctcagcaaa aactggtcaa tatggttccg gaagagctgc
     1801 gggatatctt tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgctgg
     1861 tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa
     1921 ataatgaatt cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc
     1981 aggagatgga ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga
     2041 ttagccagga ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc
     2101 ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac
     2161 cgccatcacg ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt
     2221 tcccggacct aaaaccagtg tgttaacagg tgaagaaacc ggtgtcataa aggcggcgga
     2281 tgctgccatc gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc
     2341 agccagcgca atcggagcca tcaacaccgc cgtcgcggta ttagagataa atagcccaat
     2401 aaccgccgac aagacaaaca aacaccccag catcatatgt ggcccgtaac caccgccaat
     2461 gtccatcagc cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa
     2521 cggcatcatc ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc
     2581 agcatctata cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt
     2641 aggaatttca tctgtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg
     2701 tgcctggctg tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa
     2761 gtcgcggccc tgtttggcca gcataccgat cagtttccag ttacccacaa ccaggatgat
     2821 atcgcccagc agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt
     2881 tagccccacc acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc
     2941 aatcagttct gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc
     3001 agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg
     3061 gagatcgaca tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga
     3121 aaccccatta acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac
     3181 gttagcgcca taacgctcac gtaatttgag atcatccagc cgttgaccaa tcattggcga
     3241 tccggggcga atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc
     3301 gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat
     3361 gaaacgcatc actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt
     3421 tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag
     3481 gttcggcggc gtcgccacca gcgtcatcat gccgcttatc agcccggcaa aactcaacgg
     3541 catcatcaga cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat
     3601 aaagatagcg acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat
     3661 caacaaaacc aacattttga tttcactgtt gcccgcaact ttgaccagcc atgttcccat
     3721 tacggtggca acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag
     3781 gacaacgtta ggatcagaaa agccggaaaa tacttcgggg actgtcagcg ttccgcttaa
     3841 tgcaaacgcg acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa
     3901 caagacaatg gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta
     3961 tccttgttaa ttgagggaga tgtcttgatt ctgccataaa aaagccccga cgagacgggg
     4021 ctaaatcatg atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca
     4081 gtaatttgct ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc
     4141 ggagcgttaa ccgcaatgac atgacaaccc gccgccaggc cagaaagcac gccagcggag
     4201 catcttccac caccacacac tcctgcggcg caagccccag cagctgcgcg cctaacagat
     4261 acgcatcagg ttctggtttt ccgcgcttca ctcgctcagc ggttacaaac acctctggtg
     4321 cgggaagccc agctatttta tggcgcgctc gcgctaccgg catggagcca gaagtcacaa
     4381 tggcccacgg aatacctgct ttattcaaat gactgagtaa ggcgattgcc cccggaagcg
     4441 ggtaatacct tcggtttccg tggcctcgat gtgctccaga cgcgtaaact cggcggcaat
     4501 atcagcctcg gatttgcccg ccataaaatg gcgcagagag gtgatcgcct gtttaccgtg
     4561 aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt tgctccacgc
     4621 ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca gaaaaccttt
     4681 gcaccgcacg cgggcctcct caggcattga taatttgatt aatttcgttg gcgctcaaat
     4741 ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt tcccacattg
     4801 gcgtctgggc gttaaaaccg tgagttccgg cgtcaaaatg ggtatagcgc ccttccacat
     4861 taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga
     4921 aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag gaaacatgca
     4981 acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct ttcagctcgc
     5041 caaactcgcg atccaattca cgcatctgta atccgtaacc tcgctcaata attgtttgca
     5101 ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg tactgattag
     5161 acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc actctactac
     5221 ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg ggtcaattac
     5281 aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat
     5341 gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc ctgcaattta
     5401 ccgcgaactt cataaaacca gttaaggatt gcagggggta atggcgttac agaacgcttg
     5461 cccagccacc acaatccctg catgggtaaa cttaaggcga acagtgcagt ggcaactgcc
     5521 ggcccaagct gaccgcccag ggcaatctgc cagcagagag taaatacggc gatcggcggc
     5581 ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa gaccggggca
     5641 aggcgttttt ccagtggcca cgtctttgag taatgctgtc cgcggcgaaa caagctaaaa
     5701 aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac atataaagat
     5761 tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt ttgtcttcca
     5821 tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata ttcattgagt
     5881 cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc atagactcaa
     5941 gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg
     6001 tgtcatcatg cgctacgctc tatggctccc tgacgttttt ttagccacgt atcaattata
     6061 ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc ttcactgaaa
     6121 tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc
     6181 cacctgcctg aagcacgtat caaatggaaa atggacggca ataaacagga agcggcttta
     6241 ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat tctggcacaa
     6301 aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca cggcggcgaa
     6361 aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa agatgcagct
     6421 tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc tctgaaatct
     6481 ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca ccagactatg
     6541 ccggaagagt cttacctcta cgccctgcct tacaacctgt acaaagagca cggcatccgt
     6601 cgttacggcg cgcacggcac cagccacttc tatgtaaccc aggaagcggc aaaaatgctg
     6661 aacaaaccgg tagaagaact gaacatcatc acctgccacc tgggcaacgg tggttccgtt
     6721 tctgctatcc gcaacggtaa atgcgttgac acctctatgg gcctgacccc gctggaaggt
     6781 ctggtcatgg gtacccgttc tggtgatatc gatccggcga tcatcttcca cctgcacgac
     6841 accctgggca tgagcgttga cgcaatcaac aaactgctga ccaaagagtc tggcctgctg
     6901 ggtctgaccg aagtgaccag cgactgccgc tatgttgaag acaactacgc gacgaaagaa
     6961 gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg cgaaatacat cggtgcctac
     7021 actgcgctga tggatggtcg tctggacgct gttgtattca ctggtggtat cggtgaaaat
     7081 gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg tgctgggctt tgaagttgat
     7141 catgaacgca acctggctgc acgtttcggc aaatctggtt tcatcaacaa agaaggtacc
     7201 cgtcctgcgg tggttatccc aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc
     7261 ctgactgcct gatttcacac cgccagctca gctggcggtg ctgttttgta acccgccaaa
     7321 tcggcggtaa cgaaagagga taaaccgtgt cccgtattat tatgctgatc cctaccggaa
     7381 ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg
     7441 ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac cggtggcgat gcgcccgatc
     7501 agactacgac tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa
     7561 tgagctacgt tgaaggtctg ctttccagca atcagaaaga tgtgctgatg gaagagatcg
     7621 tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc
     7681 cgacacgtaa gcaccagttt gcccagtctc tgaactacga aatcgctaaa acgctgaatg
     7741 cggaaatcgt cttcgttatg tctcagggca ctgacacccc ggaacagctg aaagagcgta
     7801 tcgaactgac ccgcaacagc ttcggcggtg ccaaaaacac caacatcacc ggcgttatcg
     7861 ttaacaaact gaacgcaccg gttgatgaac agggtcgtac tcgcccggat ctgtccgaga
     7921 ttttcgacga ctcttccaaa gctaaagtaa acaatgttga tccggcgaag ctgcaagaat
     7981 ccagccctct gccggttctc ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg
     8041 cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc
     8101 gccgcgttaa atccgtcact ttctgcgcac gcagcattcc gcacatgctg gagcacttcc
     8161 gtgccggttc tctgctggtg acttccgcag accgtcctga cgtgctggtg gccgcttgcc
     8221 tggcagccat gaacggcgta gaaatcggtg ccctgctgct gactggcggc tacgaaatgg
     8281 acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg
     8341 tgaacaccaa cacctggcag acctctctga gcctgcagag cttcaacctg gaagttccgg
     8401 ttgacgatca cgaacgtatc gagaaagttc aggaatacgt tgctaactac atcaacgctg
     8461 actggatcga atctctgact gccacttctg agcgcagccg tcgtctgtct ccgcctgcgt
     8521 tccgttatca gctgactgaa cttgcgcgca aagcgggcaa acgtatcgta ctgccggaag
     8581 gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt
     8641 gcgtactgct gggtaatccg gcagagatca accgtgttgc agcgtctcag ggtgtagaac
     8701 tgggtgcagg gattgaaatc gttgatccag aagtggttcg cgaaagctat gttggtcgtc
     8761 tggtcgaact gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag
     8821 acaacgtggt gctcggtacg ctgatgctgg aacaggatga agttgatggt ctggtttccg
     8881 gtgctgttca cactaccgca aacaccatcc gtccgccgct gcagctgatc aaaactgcac
     8941 cgggcagctc cctggtatct tccgtgttct tcatgctgct gccggaacag gtttacgttt
     9001 acggtgactg tgcgatcaac ccggatccga ccgctgaaca gctggcagaa atcgcgattc
     9061 agtccgctga ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg ctctcctact
     9121 ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg
     9181 cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct gcagtacgac gctgcggtaa
     9241 tggctgacgt tgcgaaatcc aaagcgccga actctccggt tgcaggtcgc gctaccgtgt
     9301 ttatcttccc ggatctgaac accggtaaca ccacctacaa agcggtacag cgttctgccg
     9361 acctgatctc catcgggccg atgctgcagg gtatgcgcaa gccggttaac gacctgtccc
     9421 gtggcgcact ggttgacgat atcgtctaca ccatcgcgct gactgcgatt cagtctgcac
     9481 agcagcagta acctcgtcat catccgcagc tttgcgctgc ggatatctga accggaaata
     9541 atcactattt ccggtttttt attctcttaa tctgcattaa tcctttctga ttatcttgct
     9601 taactgcgct gcatcaatga attgcgccat ttcactttac atacttacca ctttgttttg
     9661 tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc caaaccaaca agaagatggg
     9721 caatgcccga tacgttggtg attatctttt ttgttgctat tttaaccagc cttgccacct
     9781 gggtagttcc ggtggggatg tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa
     9841 caaaaacacg caaagtcgta gatccacact catttcgcat tctgactaac gaagcaggcg
     9901 aacctgagta tcaccgcgta cagctgttca cgacgggcga tgaacgcccg ggcctgatga
     9961 acttcccgtt tgaaggatta acctcaggat cgaaatacgg gacagctgtt ggcatcatca
    10021 tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga accattgata
    10081 acggtatcct ggcgcttatt cgccataccc gcgggaatga aattctcttt attcctgcgc
    10141 tgtttattct gttttcactt ggcggcgcgg tatttggtat gggagaagag gccgtcgcct
    10201 ttgccattat catcgcaccg ctaatggtcc ggctgggcta tgacagtatt accaccgtcc
    10261 tggtgaccta tattgccacg caaatcggtt ttgccagttc gtggatgaac ccgttttgtg
    10321 tggtcgttgc tcaggggatt gccggcgttc cggtgctttc tggctccggg ttgcgcatcg
    10381 tggtgtgggt tatcgccact atgattggcc tgatctttac catggtgtac gcctcacgag
    10441 tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc
    10501 aggcggatgt tgaacaacgt ccgtttacct ttggtgactg gctggtattg attgtcctga
    10561 ccgccgtaat ggtctgggtg atttggggcg tgatcgttaa tgcctggttt attccagaaa
    10621 ttgccagcca gttcttcacc atgggtctgg tgattggcat catcggcgtt gttttccgcc
    10681 ttaacggcat gacggttaat accatggctt catcctttac cgaagggacg cgaatgatga
    10741 tcgcccctgc cctgctggtg ggtttcgcca aagggatttt gctgctggtc ggtaatggtg
    10801 aagcgggtga tgccagcgtg ttaaatacca tcctcaacag cattgccaat gccattagcg
    10861 gtctggacaa cgcggtcgcg gcctggttta tgttgctctt ccaggcggta tttaatttct
    10921 tcgtgacgtc cggttctggt caggcggcgt taaccatgcc gttactggca ccgcttggcg
    10981 atctggtcgg tgttaaccgt caggttaccg tgctggcatt ccagtttggt gatggcttca
    11041 gtcacatcat ttacccaacc tcagcttcgt taatggcaac tctcggtgtg tgcagggtgg
    11101 acttccgtaa ctggctgaag gtgggcgcga ccctacttgg actgctgttt attatgtcca
    11161 gcgtcgtagt gatcggcgct cagttgatgg gctaccacta aaaatgttaa gagccgcaat
    11221 tgcggctctt ttttcattct gccgtttcag tctctacggc ttcatttttg gcattgcgct
    11281 tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga
    11341 tttcttccgg cgtcagccag caaacttcac tgacttcatc ttcctgtagg gcgaagggac
    11401 cgtgagagac gcagctgaac aacgcgcccc agacacggca atttttatct tcgaaataga
    11461 actgcccgtg ctcggcaaag gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac
    11521 gcgcggattc cagcagttgc tcatcggcct gaaccacacc gcctgcggtc gcatctaaca
    11581 tgccgggtaa aaagtctttt gtctcggtac gacgctggac cagaattttg cccatgccat
    11641 catgcacgac gatgtaagtt gcacgatgac gcagacactg tgcccgcatt tgttcccggc
    11701 tggcttgtgc aatgacttcg ttctcttcat tgacaatatc cacccattca gtacttgcca
    11761 aacgacgctg ttccattatc aggaaacctt ctttttctgg cgctcttacg gcgcattttg
    11821 agttgtgggt aaattacgga ttaatcgcga cctgcgcaat gatactttga tcattgagtg
    11881 cgattacact aagtacgtca ttatccagca tgccataact cgccggatta ccgcctttcg
    11941 gaatactcac cgagccgggg ttgaagtgga aaatctcccc ccgctgttct gccactggca
    12001 gatgggtatg accgtacacc agcacatcgt tctggtttaa agcaggtaga ttttccgggc
    12061 caaaaagatg accatgcgtc aaaaacagac gttgtttttc cagtaatacc tgttgccacg
    12121 gcgcggttat cgggaaatgc agcagcattt gatccacttc gctgtcgcag ttgccgcgca
    12181 cagcgataac cttatgtgcc acttcattaa gccgttcagc gactttggct ggcgcgtaac
    12241 cctccggtaa agcattacgc gggccgtgat tcaacacgtc gccaaggatc accagccact
    12301 gggcaccgct ttgggcaaac aactccagaa cacgttccgt cgccggtaac gacccatgaa
    12361 tgtccgatgc aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt aagctatgat
    12421 actggattct gtcgttgcta tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg
    12481 acagacgctc gctgccactg gaatgtcgca taatccacca ggcgttctgg cacctcatcg
    12541 ccatgtagta ccaggcggtt aatcattagc gccagatcag tatcagcaat gcaccattca
    12601 ccaaataaat tcggctgacc cagtgctaac aaatgttctg ccatcgcgaa cagtttctct
    12661 gcactggctt ttccctcggc cgttagtggc gcttttttcg cccccgcaaa gacaacatcc
    12721 gtcggacgct cttcgcggat gggcatcaga tcgctgcgca gccaggcctg aatccgtcgt
    12781 gcacgcgcac gattttctaa atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat
    12841 cgatcttcca gatactccgc aatggcagaa gattcactca actcaaaatc atcgatttgt
    12901 aataacggca cacggcgtgt ctgaccgtaa ccttgccacg tcggcttcaa atgttcaccg
    12961 ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt tttcctgcaa cgccacccag
    13021 gcggataaca cataagggga gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta
    13081 ctcattatgt cctcggcaat aaagggggct ggctatcaaa ctatagagcc tttttgtcac
    13141 tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg
    13201 attaaacaac cggagctgca atgatcgatc tctatttcgc cccgacaccc aatggtcaca
    13261 aaattacgct gtttctcgaa gaagcagagc ttgattatcg cttgattaag gtagacctgg
    13321 ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc gcccaacaac aaaattccgg
    13381 caattgttga tcattctcct gccgatggcg gcgaaccgct aagcctcttt gagtctggtg
    13441 ccattttgtt gtatctgact gagaaaacag gactcttttt gagtcatgaa acgcgtgagc
    13501 gcgccgccac attacagtgg ttattctggc aggtaggcgg actggggccg atgcttgggc
    13561 aaaatcatca ttttaatcac gcagcccccc aaaccattcc ttacgctatt gaacgttatc
    13621 aggttgaaac tcagcgtctt taccatgtac taaacaagcg gctggaaaac tcgccctggc
    13681 tgggaggcga gaactacagc attgcggata ttgcctgctg gccgtgggtt aatgcctgga
    13741 ctcgccagcg aattgacctc gcaatgtatc cggcagtcaa gaactggcat gagcggatcc
    13801 gttcgcgccc tgccaccggg caggcactgc taaaagcaca actcggtgat gagcgttcgg
    13861 atagttaaca gaaacaggtt ctcgtgtatt atttcatcct aagtaaaaca acggagaacc
    13921 tgcaatggca caacctgccg ctattattcg tataaagaac cttcgtttac gtacgtttat
    13981 cggaattaag gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg tgacgatcca
    14041 ctaccccgcc gataaagcgc gcactagcga agatatcaac gatgcgctga attatcgcac
    14101 cgtaacgaaa aacattattc agcatgtaga gaataaccgt ttctctttgt tggaaaaatt
    14161 aactcaggat gtgctcgata tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga
    14221 gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta tccatgacct taagctggca
    14281 gcgttaaccg ccatatcggg aggctgcatg aacatagtga tcaccggagg gacgggatta
    14341 attggtcgcc atttgattcc gcgtttgctg gagctgggcc atcaaatcac ggtagtgacg
    14401 cgtaacccac agaaagccag ttccgttctc ggccctcggg tgacgctatg gcaagggcta
    14461 gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca acctggccgg agaaccgatt
    14521 gctgataaac gctggactca cgagcaaaaa gagcgtctct gccaaagccg ctggaatatc
    14581 acgcaaaaac tggtcgattt gattaatgcc agcgacacgc cgccgtcggt attcatttcc
    14641 ggctcggcaa cgggctatta tggcgactta ggtgaagtgg tggttaccga agaggaaccg
    14701 ccgcataacg aatttactca taaactctgc gcccgctggg aagagattgc ctgccgggcg
    14761 caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg tggtactggc accggacggc
    14821 ggtattctcg gtaaaatgct gccaccgttt cgtcttggcc tgggcgggcc gattggttcc
    14881 ggtcggcagt atctggcctg gattcatatt gatgatatgg tcaacggcat tctctggctg
    14941 ctggataacg acctgcgcgg gccatttaat atggtttcgc cctacccggt acgcaatgaa
    15001 caatttgccc atgcgctcgg tcaagcgctg catcgcccgg ccattttgcg cgtccccgca
    15061 accgccattc ggctgttaat gggcgaatct tcaatactgg tattaggtgg acaacgcgcg
    15121 ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct ggtacgattt agaagaggcg
    15181 ctggcggatg tcgttcgctg atgtggttta cagcaaacat ccgccagtta actcccggtg
    15241 ttacaggatt agtggctttg cgcgataaga tcgtctggtg aaagtcgggt caccatcata
    15301 actaactctc tgtctaaacc tctatccagc atctcctgag caatacgcag ggcttcttcg
    15361 tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag caatggtcat cagtttctcc
    15421 ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg cacggaaacg ctgggcatcc
    15481 cctgtttgta atacgtaatt aaacagggct tttagctgtc tgtcattagt gttccctgta
    15541 actagcagcg aaacaatttg gtcgactaat cccaacagat cgcgctgacg aatatgtttc
    15601 tgaattaact ccaacagcgc cattttgcgg tgttgcataa tctcgtcatc cggcaccacg
    15661 gtaatatcca ccaacggaaa cgctgatgaa tatattttgc gggctatagc aggctcggca
    15721 aattcatcaa gccagcagag tgaataagga taaggacttc tgcaaccatg ataaaacagc
    15781 atcgggatca ccaatggaag ctctttatag cccgcatcaa gatggttttg cattgccgca
    15841 atggaataac gcatcatgcg aaaagccatt aattcttcca gcttactttg gtgctctatc
    15901 actacataaa tataacccac tccctcctgc gttttcacag accacaagag gtcggaataa
    15961 tattgccgca ggtcttcatc aataaaactg tttgattcta gtttaagcgt cgttaaatca
    16021 cacagtttgc gcagcggcgc gggaagatga atatcaataa aatcccgcgc ggtgt
//
LOCUS       DAZOZD010000083        15848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOZD010000083 DAZOZD010000000
VERSION     DAZOZD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.083"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            351..755
                     /gene="sdhC"
                     /locus_tag="ACHUPB_002140"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             351..755
                     /gene="sdhC"
                     /locus_tag="ACHUPB_002140"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0758950.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            749..1096
                     /gene="sdhD"
                     /locus_tag="ACHUPB_002141"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             749..1096
                     /gene="sdhD"
                     /locus_tag="ACHUPB_002141"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0758951.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1096..2862
                     /gene="sdhA"
                     /locus_tag="ACHUPB_002142"
     CDS             1096..2862
                     /gene="sdhA"
                     /locus_tag="ACHUPB_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0758952.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2878..3594
                     /gene="sdhB"
                     /locus_tag="ACHUPB_002143"
     CDS             2878..3594
                     /gene="sdhB"
                     /locus_tag="ACHUPB_002143"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0758953.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3895..6696
                     /gene="sucA"
                     /locus_tag="ACHUPB_002144"
     CDS             3895..6696
                     /gene="sucA"
                     /locus_tag="ACHUPB_002144"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0758954.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6711..7928
                     /gene="odhB"
                     /locus_tag="ACHUPB_002145"
     CDS             6711..7928
                     /gene="odhB"
                     /locus_tag="ACHUPB_002145"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0758955.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8022..9188
                     /gene="sucC"
                     /locus_tag="ACHUPB_002146"
     CDS             8022..9188
                     /gene="sucC"
                     /locus_tag="ACHUPB_002146"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0758956.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9188..10057
                     /gene="sucD"
                     /locus_tag="ACHUPB_002147"
     CDS             9188..10057
                     /gene="sucD"
                     /locus_tag="ACHUPB_002147"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0758957.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10161..10883)
                     /gene="mngR"
                     /locus_tag="ACHUPB_002148"
     CDS             complement(10161..10883)
                     /gene="mngR"
                     /locus_tag="ACHUPB_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0758958.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            11052..12968
                     /gene="mngA"
                     /locus_tag="ACHUPB_002149"
     CDS             11052..12968
                     /gene="mngA"
                     /locus_tag="ACHUPB_002149"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0758959.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            12986..15619
                     /gene="mngB"
                     /locus_tag="ACHUPB_002150"
     CDS             12986..15619
                     /gene="mngB"
                     /locus_tag="ACHUPB_002150"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR0758960.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            15644..>15848
                     /locus_tag="ACHUPB_002151"
     CDS             15644..>15848
                     /locus_tag="ACHUPB_002151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758961.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIIT"
BASE COUNT         3794 a         3969 c         4363 g         3722 t
ORIGIN      
        1 actttgttga atgattgtca aattagatga ttaaaaatta aataaatgtt gttatcgtga
       61 cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt gtaatgattt
      121 tgtgaacagc ctatactgcc gccaggtctc cggaacaccc tgcaatcccg agccacccag
      181 cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg cagttataga
      241 aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt ctccaggtaa
      301 cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc atgtgggcgt
      361 tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggaccta cagaccatcc
      421 ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg atcacctttg
      481 ttgcagtggg catcctgctg tggcttctgg gtaccagcct ctcttcccct gaaggtttcg
      541 agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg ggcatcctta
      601 ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat tttggctatc
      661 tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt attactgtcg
      721 tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat taggacgcaa
      781 tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc tctacatcat
      841 ttatatggtc ggttttttcg ccaccagtgg cgaactgaca tatgaagtct ggatcggttt
      901 cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta tcttgattca
      961 tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg cattgcgcct
     1021 gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg gattcgttgt
     1081 ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt gattggtgcc
     1141 ggtggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac ctgtgcgctg
     1201 ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg cattaccgtt
     1261 gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac cgtaaaaggg
     1321 tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg gccggaagcg
     1381 attctggaac tcgaacacat gggcctgccg ttctcgcgtc ttgatgatgg tcgtatctat
     1441 caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc acgcactgcg
     1501 gcggcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca gaacctgaaa
     1561 aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaaaaa ccaggatggc
     1621 gcggtggtgg gttgtaccgc actgtgcatc gaaaccggtg aagtggttta tttcaaagcc
     1681 cgcgctaccg tgctggcgac tggcggggca gggcgtattt atcagtccac cactaacgcc
     1741 cacattaaca ctggcgacgg tgtcggcatg gctatccgcg caggcgtacc ggtgcaggat
     1801 atggaaatgt ggcaattcca cccgaccggc attgccggtg cgggcgtact ggtcaccgaa
     1861 ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt tatggagcgt
     1921 tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc catcatgatt
     1981 gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa actgaaactc
     2041 gatcacctgg gtaaagaagt tctcgaatcc cgtctgccgg gtatcctgga gctttcccgt
     2101 acctttgctc acgtcgatcc ggtgaaagag ccgattccgg ttatcccaac ctgtcactac
     2161 atgatgggcg gtattccgac caaagtgacc ggtcaggcgc tgacggtgaa cgagaaaggc
     2221 gaagatgtgg ttgttccggg gctgtttgcc gttggtgaaa tcgcttgtgt atcggtacac
     2281 ggcgctaacc gtctgggcgg caactcgctg ctggacctgg tggtctttgg tcgtgcggca
     2341 ggtctgcatc tgcaagagtc tatcgccgaa cagggcgcat tgcgcgatgc cagcgagtct
     2401 gatgttgaag catctctgga tcgtctgcac cgctggaaca ataatcgtaa cggtgaagat
     2461 ccggtggcga ttcgtaaagc gctgcaagaa tgtatgcagc ataacttctc ggtcttccgt
     2521 gaaggtgatg cgatggcgaa agggcttgag cagttgaaag tgatccgcga gcgtctgaaa
     2581 aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga gtgcttggaa
     2641 ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt ccgtaccgaa
     2701 agccgtggcg cacatagccg cttcgacttc ccggatcgtg atgatgaaaa ctggctgtgc
     2761 cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa catggaaccg
     2821 aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga caggaaaatg
     2881 agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc gcgtatgcag
     2941 gattaccccc tggaagcgga agaaggtcgc gacatgatgc tgctggatgc gcttatccag
     3001 ctgaaagaga aagatcccag cctgtcgttc cgccgctcct gccgtgaagg tgtgtgcggt
     3061 tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc gatttcggca
     3121 ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc ggtgatccgc
     3181 gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa gccttacctg
     3241 ttgaataatg gacaaaatcc gccagctcgc gagcatttac agatgccaga gcagcgcgaa
     3301 aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc ttgtccgtct
     3361 ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc atatcgtttc
     3421 ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag cgatgcattc
     3481 agcgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa ggggctgaac
     3541 ccgacgcgcg ccatcggcca tattaagtcg atgttgttgc aacgtaatgc gtaaaccgta
     3601 ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg gcgtgaacgc
     3661 cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat caggcgtaac
     3721 aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt ttaaaggttc
     3781 cttcgcgagc cactacatag acaagagctc gcaagtgaac cccggcacgc acatcactgt
     3841 gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat cacgatgcag
     3901 aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa ccagagctgg
     3961 atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc taactggcgt
     4021 tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca ctctcaaacg
     4081 cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac gatctccgac
     4141 cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt
     4201 ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa agtggccgat
     4261 ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggagacctt caacgtcggt
     4321 tcatttgcca gcggcaaaga aaacatgaaa ctcggcgagc tgctggaagc cctcaagcaa
     4381 acctactgtg gcccgattgg tgccgagtat atgcacatta ccagcaccga agaaaaacgc
     4441 tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc
     4501 ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggcgc aaaattccct
     4561 ggcgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct taaagagatg
     4621 atccgccacg ctggcaacag cggcacccgc gaagtggttc ttgggatggc gcaccgtggt
     4681 cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttt
     4741 gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat gggcttctcg
     4801 tctgacttcc agaccgatgg cggcctggtg cacctggcgc tggcgtttaa cccgtctcac
     4861 cttgagattg taagcccggt agttatcggt tctgttcgtg cccgtctgga cagacttgat
     4921 gagccgagca gcaacaaagt gctgccaatc accatccacg gtgacgccgc agtgaccggg
     4981 cagggcgtgg ttcaggaaac tctgaacatg tcgaaagcgc gtggttatga agttggcggt
     5041 acggtacgta tcgttatcaa caaccaggtt ggtttcacca cctctaatcc actggatgct
     5101 cgttctacac cgtactgtac tgatatcggt aagatggttc aggcaccgat tttccacgtt
     5161 aacgcggatg atccggaagc cgttgccttt gtgacccgtc tggcgctcga tttccgtaac
     5221 acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg ccacaacgaa
     5281 gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa acatccgaca
     5341 ccgcgcaaaa tctacgctga caagctggag caggaaaaag tggcgacgct ggaagatgcc
     5401 accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt agtggcagag
     5461 tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca cgaatgggac
     5521 gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg agctggcgaa acgcatcagc
     5581 acggtgccgg aagcagttga aatgcagtct cgcgttgcca agatttatgg cgatcgccag
     5641 gcgatggctg ccggtgagaa actgttcgac tggggcggtg cggaaaacct cgcttacgcc
     5701 acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg tcgcggtacc
     5761 ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta cacgccgttg
     5821 caacacatcc ataacgggca gggcgcgttc cgcgtctggg actccgtact gtctgaagaa
     5881 gcagtgctgg cgtttgaata cggttatgcc accgcagaac cacgcactct gaccatctgg
     5941 gaagcgcagt tcggtgactt cgccaacggt gcgcaggtgg ttatcgacca gttcatctct
     6001 tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc gcacggttac
     6061 gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca actttgtgct
     6121 gagcaaaaca tgcaggtttg cgttccgtct accccggcac aggtttacca catgctgcgt
     6181 cgtcaggcgc tgcgcgggat gcgtcgtccg ctggtagtga tgtcgccgaa atccctgctg
     6241 cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt cctgccagcc
     6301 atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat gtgttctggt
     6361 aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga tgtcgccatt
     6421 gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt gttgcagcag
     6481 tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca gggcgcatgg
     6541 tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct gcgttatgca
     6601 ggccgcacgg cctccgcctc tccggcggta gggtatatgt ccgttcacca gaaacagcaa
     6661 caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca atgagtagcg
     6721 tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc
     6781 ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca
     6841 aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt ctggaagatg
     6901 aaggtacaac ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgccg
     6961 gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa cgccagcagg
     7021 cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc ctgctggctg
     7081 aacacaatct cgacgccagc gccattaaag gcaccggtgt gggtggtcgt ctgactcgtg
     7141 aagatgtgga aaaacatctg gcgaaagccc cggcgaaaga gtctgctccg gcagcggctg
     7201 ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg atgactcgcc
     7261 tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca
     7321 cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac ggtgaagcgt
     7381 ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg
     7441 aagccctgaa acgttacccg gaagtgaatg cttctatcga cggtgatgac gtggtttacc
     7501 acaactattt cgacgtcagc atggcggttt ccacgccgcg cggcctggtg acgccggtac
     7561 tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa gagctggcag
     7621 ttaaaggccg tgacggcaag ctgacggttg aagatctgac cggtggtaac ttcaccatca
     7681 ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg ccgcagagcg
     7741 caattctggg tatgcacgct atcaaagatc gtccgatggc ggtgaatggt caggttgaga
     7801 tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat ggtcgcgaat
     7861 ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt ctgctgctgg
     7921 acgtgtagta gtttaagctt cacctgcact gtagaccgac gtggataggg tgtcggtcta
     7981 cggtttaaaa gataacgatt actgaaggat ggacagaaca catgaactta catgaatatc
     8041 aggcaaaaca actttttgcc cgctatggct taccagcacc ggtgggttat gcctgtacta
     8101 ctccgcgcga agcagaagaa gccgcttcaa aaatcggtgc cggcccgtgg gtagtgaaat
     8161 gtcaggttca cgctggtggc cgcggtaaag cgggcggtgt gaaagttgta aacagcaaag
     8221 aagacatccg tgcttttgca gaaaactggc tcggcaagcg tctggtaacg tatcaaacag
     8281 atgccaatgg ccaaccggtt aaccagattc tggttgaagc agcgaccgat atcgctaaag
     8341 agctgtatct cggtgccgtt gtagaccgta gttcccgtcg tgtggtcttt atggcctcca
     8401 ccgaaggcgg cgtggaaatc gaaaaagtgg cggaagaaac tccgcacctg atccacaaag
     8461 ttgcgcttga tccgctgact ggcccgatgc cgtatcaggg acgcgagctg gcgttcaaac
     8521 tgggtctgga aggtaaactg gttcagcagt tcaccaaaat cttcatgggc ctggcgacca
     8581 ttttcctgga gcgcgacctg gcgttgatcg aaatcaaccc gctggtcatc accaaacagg
     8641 gcgatctgat ttgcctcgac ggcaaactgg gcgctgacgg caacgcactg ttccgccagc
     8701 ctgatctgcg cgaaatgcgt gaccagtcgc aggaagatcc gcgtgaagca caggctgcac
     8761 agtgggaact gaactacgtt gcgctggacg gtaacatcgg ttgtatggtt aacggcgcag
     8821 gtctggcgat gggtacgatg gacatcgtta aactgcacgg cggcgaaccg gctaacttcc
     8881 ttgacgttgg cggcggcgca accaaagaac gtgtaaccga agcgttcaaa atcatcctct
     8941 ctgacgacaa agtgaaagcc gttctggtta acatcttcgg cggtatcgtt cgttgcgacc
     9001 tgatcgctga cggtatcatc ggcgcggtag cagaagtggg tgttaacgta ccggtcgtgg
     9061 tacgtctgga aggtaacaac gccgaactcg gcgcgaagaa actggctgac agcggcctga
     9121 atattattgc agcaaaaggt ctgacggatg cagctcagca ggttgttgcc gcagtggagg
     9181 ggaaataatg tccattttaa ttgataaaaa caccaaggtt atctgccagg gctttactgg
     9241 tagccagggg actttccact cagaacaggc cattgcatac ggcactaaaa tggttggcgg
     9301 cgtaacccca ggtaaaggcg gtactaccca cctcggcctg ccggtgttca acaccgtgcg
     9361 tgaagctgtt gctgctactg gcgctaccgc ttctgttatc tacgtaccag caccgttctg
     9421 caaagactcc attctggaag ctattgatgc aggcatcaaa ctgattatca ccatcactga
     9481 aggcatcccg acgctggata tgctgactgt gaaagtgaaa ctggacgaag ccggcgttcg
     9541 tatgatcggc ccgaactgcc cgggcgttat cactccgggt gaatgcaaaa tcggtatcca
     9601 gccgggtcac attcacaaac cgggtaaagt gggtatcgtt tcccgttccg gtacactgac
     9661 ctatgaagcg gttaaacaga ccacggatta cggtttcggt cagtcgacct gtgttggtat
     9721 cggcggtgac ccgatcccag gctctaactt tatcgacatc ctcgaaatgt tcgaaaaaga
     9781 tccgcagacc gaagcgatcg tgatgatcgg tgagatcggc ggtagcgctg aagaagaagc
     9841 ggcagcgtac atcaaagagc acgttaccaa gccagttgta ggttacatcg ctggtgtgac
     9901 tgcgccgaaa ggcaaacgta tgggccacgc gggtgcgatc attgctggtg ggaaagggac
     9961 tgcggatgag aaattcgctg ctctggaagc cgcaggcgtg aaaaccgttc gcagcctggc
    10021 ggatatcggt gaagcactga aaactgttct gaaataaata tctgtaataa gaaatagccc
    10081 tcgccgcttc cctctacagg aatggcgaag ggctgtcggt ttcgacatgg ttggccatcg
    10141 tatgatggcc ttttttgtgc ttatcgcgat gattttcgct gcgctatcag ggtaaattta
    10201 tagtcatcgg tattaaaagc gttgcggcta tattcaaaca cccgaccatc aactaaatat
    10261 ccacgcgata ctttttcaag aatcggcttt gtctggctga tattaagcag acggctcatc
    10321 tcttcggttg gcatcagagg aatgatttcc tgttcgctac gatcgataac catcttcttc
    10381 acttcttcaa taaagtgata tttcgaattt tccatgacct gccaggtgag atccgggaac
    10441 aacgcaagcg gcatccaggt ttcttccagc gccattggct tttgcttgcg atagcgcacg
    10501 cgcttcacat gccacacacg atcctgcgcg gtgatttgta gctgttgctg aagaaaatcg
    10561 tcagccggaa tcacttcaaa tatcaggatt tcactgtgtg tatcgacgtg acggtccgac
    10621 agtttttcat caaaactggt taactgaaaa atatcgtaat tgacccgctc ttctttgacg
    10681 taagtcccgc tgccctgaat gctttcgagg atctgctgct cgactagctg gcgcaaagcc
    10741 tgacgcaccg taacccggct gacgccaaac tctgtttgta gcgctgattc agtgggtaac
    10801 gcatcgccag gtttaagctc gccacgcgca atttgttcac gaatgcgatc ggcaatctgc
    10861 cggtataagg gcttgtgtcc catttttagt atctcattaa tatgaattta gccattatgc
    10921 ccgataaatt catcctgtaa ataatacaaa tacaatacaa ataatttcaa tcaagtgaaa
    10981 ttgatcacat gatggtattg ttttatcggg cacactggcg cgactataaa aacgatcaag
    11041 tgaggatcat gatgaatctg acgactctga cccaccgcga tgcgttgtgt ctgaatgcgc
    11101 gctttaccag ccgtgaagag gccatccacg cgttgactca acgtcttgct gctctgggga
    11161 aaatttccag tactgagcaa tttctggaag aagtgtatcg ccgtgaaagc cttggcccga
    11221 ccgccttagg tgaagggttg gctgtgccgc atggcaaaac tgctgcggta aaagaagcgg
    11281 cgtttgcggt cgccacactc agcgagccgc ttcagtggga aggcgttgat ggcccggaag
    11341 cagttgattt agtggtgctg ctggcgattc cccccaatga agcgggtact acgcatatgc
    11401 aactgctgac agcgctgacc acgcgccttg cggatgatga gattcgggcg cgtatacagt
    11461 cggcgacgac gcctgatgag ttgctctcgg cgctggatga caagggaggc acgcaacctt
    11521 ctgcctcttt ttccaacgcg ccaactatcg tctgcgtaac ggcctgtccg gcgggtattg
    11581 ctcacaccta tatggctgcg gaatatctgg aaaaagccgg acgcaaactc ggcgtaaatg
    11641 tttacgttga aaaacaaggc gctaacggca ttgaagggcg tttaacggcg gatcaactca
    11701 atagtgcaac cgcctgtatt tttgcggctg aagtcgccat caaggagagt gagcgtttta
    11761 atggcattcc cgcgctttca gtgcctgttg ccgagccgat tcgccatgca gaagcgttga
    11821 tccaacaagc gcttaccctc aagcgtagcg atgagacgcg taccgtacag caagatacgc
    11881 aaccggtgaa aagtgtcaaa acggagctga aacaggcact gttgagcgga atctcttttg
    11941 ccgtaccgtt gattgtcgcg gggggcacgg tgctggcggt cgcggtatta ctgtcgcaaa
    12001 tcttcgggct acaagatctg tttaatgaag aaaactcctg gctgtggatg taccgcaagc
    12061 tgggcggcgg gctgctcgga attttgatgg taccggtgct cgcggcctat accacctatt
    12121 ctctggcaga taaaccggcg ttagcgccag gctttgcggc tggacttgcc gccaacatga
    12181 tcggctccgg gtttctcggc gcggtcgttg gcggattgat agccggttac ttgatgcgct
    12241 gggtgaaaaa tcacttgcgt cttagcagta aattcaatgg attcctgact ttttatctct
    12301 acccggtgct cggtacgttg ggagcgggca gtctgatgct gtttgtggtg ggagaacctg
    12361 tcgcatggat caataactcg cttaccgcct ggctgaacgg tctgtcagga agtaacgcgc
    12421 tgttgctggg tgccattctc ggttttatgt gttcctttga ccttggaggg ccagtgaaca
    12481 aagccgctta tgcattctgc ctgggcgcaa tggcgaacgg cgtttacggc ccgtatgcca
    12541 ttttcgcctc cgtcaaaatg gtttcggcat ttaccgtaac cgcttccacg atgctcgcac
    12601 cgcgcctgtt taaagagttt gaaattgaga ccgggaaatc cacctggctg ttagggctgg
    12661 caggtattac cgaaggggcg atcccgatgg cgattgaaga tccgctgcgg gttattggtt
    12721 cgtttgtgct gggctctatg gtaacgggcg ctattgtcgg tgcgatgaat atcggccttt
    12781 cgacacccgg tgccggcatt ttctcgctct ttttacttca tgataatggc gcgggcggtg
    12841 ttatggcggc aattggctgg tttggtgcgg cattggtggg ggctgcaatc tcgactgcaa
    12901 ttctcctgat ctggcggcgt cacgcggtta agcatggcaa ctatctgact gatggcgtaa
    12961 tgccataaac aaaaaggaaa cgacgatgaa agcggtatct cgcgttcaca tcaccccgca
    13021 tatgcattgg gatcgggagt ggtatttcac caccgaagag tcacgcattc tgctggtcaa
    13081 taatatggaa gagatcctgt gccgactgga acaggacaac gaatacaaat attacgtact
    13141 cgacgggcaa acggcgatcc tcgaagatta tttcgcggtg aaaccggaaa acaaagaccg
    13201 cgtgaagaaa caggtagaag ccggtaagtt gattatcggc ccctggtata cccagaccga
    13261 taccacgatt gtttctgcgg aatccatcgt ccgtaatctg atgtacggaa tgcgtgactg
    13321 cctcgcgttt ggcgagccga tgaaaatagg ttatttaccg gattcctttg gcatgtccgg
    13381 gcaactgccg catatttaca atggatttgg cattacccgc actatgttct ggcgcggatg
    13441 ttcggagcgc cacggtactg ataaaaccga gtttttgtgg caaagcagtg acggtagcga
    13501 agtgacggcg caggtgctgc cgctgggcta cgccatcggt aagtacttac ctgccgacga
    13561 aaacggatta cgtaaacgcc tcgacagtta ttttgacgtg ctggaaaaag cgtctgtaac
    13621 caaagagatt ttgctgccga atggtcatga ccagatgcca ttgcagcaaa atatcttcga
    13681 agtgatggat aagctacgtg agatctaccc tcaacgtaag tttgtgatga gccgctttga
    13741 agaggtattt gagaagatcg aagcgcagcg tgaaagtctg gccaccctga aaggtgaatt
    13801 tatcgatggc aaatatatgc gcgtacatcg caccatcggt tccacgcgca tggatatcaa
    13861 aattgcccac gcacgtattg aaaataagat tgttaatctg ctggaaccgc tggcaacgct
    13921 ggcctggacg ttgggttttg aataccacca cggcttgctg gagaaaatgt ggaaagagat
    13981 cttaaaaaat catgcccacg acagtatcgg ttgttgttgc agtgacaaag ttcatcgcga
    14041 aattgtcgcc cgcttcgaac tggctgaaga catggcggat aatctgctgc gtttctacat
    14101 gcgcaaaatt gccgataaca tgccacagag tgatgccgac aaactcgtgc tgtttaacct
    14161 gatgccctgg ccgcgggaag aagttattaa caccactgtg cggctgcgcg ccagccagtt
    14221 taatctacaa gacgatcgcg gtcagcctgt accgtatttt attcgccatg cccgtgagat
    14281 cgatcctggc ctaatcgatc ggcaaattgt tcattatggc aattatgatc cctttatgga
    14341 gtttgatatt cagatcaacc agattgtccc ttctatgggc tatcgcacgc tttatattga
    14401 agcgaatcag cctggcaacg tgattgcggc aaaaagtgac gctgaaggga ttctggaaaa
    14461 tgctttctgg cagattgcgc tcaatgagga tggttctctg caactggtag ataaagacag
    14521 cggtgtgcgc tatgaccggg tattgcaaat tgaagaaagc tctgatgatg gtgatgaata
    14581 tgactattca cccgcaaaag aagagtgggt aattaccgca gcgaacgcga aaccgcaatg
    14641 cgatattatt catgaagcct ggcagagcag ggctgttatc cgctatgaca tggcagtgcc
    14701 gctcaatttg tcagaacgca gcgcccggca atccactggc agagtagggg gggtgttggt
    14761 tgtcactctt agtcataaca gcaggcgtat tgatgtggat atcaaccttg ataaccaggc
    14821 tgacgatcat cgccttcgtg tcctggtccc tacacctttt aacactgaca gtgttctggc
    14881 agatacgcag tttggttcgc taacgcgccc cgtgaacgac agtgcaatga acaactggca
    14941 gcaagaaggc tggaaagaag cgccggttcc ggtatggaat atgctcaact atgttgcctt
    15001 acaggaaggg cgtaacggca tggctgtctt tagcgaaggg ttacgtgaat ttgaagtcat
    15061 cggtgaagag aagaaaacct ttgccattac gttgctgcgt ggcgtgggct tactgggcaa
    15121 agaagatctg cttttaaggc ctgggcggcc ttcgggaatt aaaatgccag tcccggactc
    15181 acaactacgt ggtctgcttt cttgtcgcct aagtttattg agttataccg gtacgccaac
    15241 cgccgctggt gtagctcagc aggcgcgagc atggctgact ccagtacagt gttacaacaa
    15301 aatcccatgg gatgcgatga agctcaacaa agccggattc aacgtgccgg aaagttatag
    15361 tttgttgaaa atgcccccag tgggatgcct gataagcgca cttaagaaag ctgaagaccg
    15421 acaagaagtg attttacggc tgtttaatcc ggctgaatca gcaacctgtg atgcgactgt
    15481 tgctttcagt cgcgaggtga tttcttgctc agaaacgatg atggatgaac acattaccac
    15541 cgaggaaaat caaggttcaa atctatcggg gcctttttta cccggccagt cacggacgtt
    15601 cagttaccgg cttgcctgaa tagcaatcaa accgaagcca catatgcgcg gccagattgt
    15661 tgacaaaggg cgctttgttc atgccggata cggcatgaac gctttattcg gtctacaaaa
    15721 gcaggcaaat tcaatatatt gcagagatga tgtagacact ggcaagcgta gcgcatcagg
    15781 taattttgcg tttatcttca ctctcaagcc acgtatatgt ggctttattt ttaacaaaat
    15841 aataaccc
//
LOCUS       DAZOZD010000084        15791 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOZD010000084 DAZOZD010000000
VERSION     DAZOZD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.084"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            20..1606
                     /locus_tag="ACHUPB_002152"
     CDS             20..1606
                     /locus_tag="ACHUPB_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR0758962.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(1744..1896)
                     /locus_tag="ACHUPB_002153"
     CDS             complement(1744..1896)
                     /locus_tag="ACHUPB_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0758963.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVLVATL
                     AYEVKR"
     gene            2226..2468
                     /locus_tag="ACHUPB_002154"
     CDS             2226..2468
                     /locus_tag="ACHUPB_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758964.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            2767..3135
                     /locus_tag="ACHUPB_002155"
     CDS             2767..3135
                     /locus_tag="ACHUPB_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758965.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            3139..3354
                     /locus_tag="ACHUPB_002156"
     CDS             3139..3354
                     /locus_tag="ACHUPB_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758966.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAVSP"
     gene            complement(3552..4175)
                     /locus_tag="ACHUPB_002157"
     CDS             complement(3552..4175)
                     /locus_tag="ACHUPB_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0758967.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            4335..4802
                     /locus_tag="ACHUPB_002158"
     CDS             4335..4802
                     /locus_tag="ACHUPB_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0758968.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            4952..5128
                     /locus_tag="ACHUPB_002159"
     CDS             4952..5128
                     /locus_tag="ACHUPB_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758969.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(5191..5286)
                     /gene="dqlB"
                     /locus_tag="ACHUPB_002160"
     CDS             complement(5191..5286)
                     /gene="dqlB"
                     /locus_tag="ACHUPB_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0758970.1"
                     /translation="MINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            6083..7363
                     /gene="trbA"
                     /locus_tag="ACHUPB_002161"
     CDS             6083..7363
                     /gene="trbA"
                     /locus_tag="ACHUPB_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR0758971.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     IGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQ
                     FFLGDRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEDAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTEKQHPADKEKEQ"
     gene            7360..8484
                     /locus_tag="ACHUPB_002162"
     CDS             7360..8484
                     /locus_tag="ACHUPB_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFR0758972.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAESPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            8465..10765
                     /gene="trbC"
                     /locus_tag="ACHUPB_002163"
     CDS             8465..10765
                     /gene="trbC"
                     /locus_tag="ACHUPB_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0758973.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHIMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNIQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTWEERRVCYITLNRPEEEHSGPEDIPSVKVILNTLLPLEILLPVAE
                     NNKSQTINNDHPQKSCGAQPTTDEKY"
     gene            complement(10986..13706)
                     /locus_tag="ACHUPB_002164"
     CDS             complement(10986..13706)
                     /locus_tag="ACHUPB_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFR0758974.1"
                     /translation="MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEI
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQK
                     AAELAHFSKDDTDPVFHYILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPKMARNGELKSCLAVHRTLAEAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVVPGERFRG
                     HSIGSQPVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYGLIQCRSLIEEMIADGDF
                     SWQACHEMFARKGLMLQEQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAAADIFERVKPECRYNPELAASDEVEPGFRRDPEIRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRT
                     TNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTRTGKNVCTDYGDRVVFYHH
                     TDRNELAEKLNLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFT
                     ITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLQQEKTWEPPSPGM"
     gene            complement(13718..14050)
                     /gene="mobA"
                     /locus_tag="ACHUPB_002165"
     CDS             complement(13718..14050)
                     /gene="mobA"
                     /locus_tag="ACHUPB_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0758975.1"
                     /translation="MSEKKTRSGSEKRQKNVLIAVRFSPEEAEIVKEKAEKNGLTVST
                     LIRKTVLGKQINARIDEDFLKELMRLGRLQKHLFVEGKRTGDKEYAEVLVAITELANT
                     LRRNLMGR"
     gene            14283..14618
                     /locus_tag="ACHUPB_002166"
     CDS             14283..14618
                     /locus_tag="ACHUPB_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0758976.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKLSRRSSSKTAPKKTDSGS
                     KDGIDMNNN"
     gene            complement(14704..15552)
                     /locus_tag="ACHUPB_002167"
     CDS             complement(14704..15552)
                     /locus_tag="ACHUPB_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0758977.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEARNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKMNDIIARHYPG
                     ALPPRV"
BASE COUNT         4121 a         4273 c         3616 g         3781 t
ORIGIN      
        1 aaaacataaa gataaaatca tgtctgaatc ccgcagcaat tttccagcac gacatggtac
       61 tccatggaat actgaagagt tacagcgtct tctcgtatcc atagcggagg gcgtctcgct
      121 aaaaaaactt actcaaacac atcaacgatc gtccggggga atacgtggag caatcaaacg
      181 cctgcttcct tctgagtttc ttgagttcaa cgatataaac acggtgaatg atctcagcaa
      241 ttacttcaac aagattcctc gccaagaaca caatagtatc atcaataatt taatatcatt
      301 gagcactgca aaaacaattg ctcatgataa agttgaaata caaaagaata gttttaaaca
      361 aaatacccca ctccccctct gccaacccaa tacagatttt aaagattata tagccgatga
      421 acatgatgtc attatgctaa ttcattctgc tatcgaaagt ttatctaata aacgacatca
      481 atatattctt ctaatgcgac tcggcacaga tgattcacct cattctctgg cagatattgc
      541 cgtcaaatta ggaatttccc gtgaaagggt aagacaactt caggaacgag cccttggctt
      601 actagctgct aaatctcgct atgaagggac acctggagct tgccttaaag cccttattaa
      661 ctcaataggt aattcaccat atgatatagc agtatggatt tatcgtattg ttcatcggga
      721 tttcgttaca tcctctgagt tagccgccaa attcatcatt tttgccgcag gcattcctaa
      781 agcaagacga gatgaaataa aaaattcgct ctcattaata tcacaattac aaaagcagaa
      841 gttacgtgaa cgaagagcag aattagcaca cgctcacgct caggaaagag cccgaatcaa
      901 gaaagaaaga gttgagttca tattcagtaa atggctagac aatacacatt ggccaaaata
      961 tatcgcacct cctccccccg tagaacagct atgttcacag agagcggtta atgatactga
     1021 catatctgga ttcttttact ctcaaaaatt aggtcgtaca gtccagtacg aatcagggct
     1081 tgagtttaaa ataatgaatc tattagaatg ttgcgatcag gttcttttct atcaagaaca
     1141 accattttct attccatatt gttttaaaaa caaatcaaaa aaatactatc cagatttact
     1201 cattgcgaca gttgatggtc ggtgcctact tatggaagtg aagccaacag accgaatgac
     1261 cttgagtctc acgagaataa aaagtaatgc aggtcgcaaa tgggcacata ctcgaggatg
     1321 gggatggttg gtcattagtg ataggttctc gttacgtcag ctggaagaac atgccatctc
     1381 aacaaacaca tggaaactta tagaggctga actgaaaaca tcaaggattt tagcatggcg
     1441 tgaaatgatg gagttacgta cacgatacga gattcatcgt ttagatctta ttgcatatat
     1501 cattcagtct ggtgctgaag tggataactt ttatcgaatt accccgaaaa tatctaataa
     1561 tacatctaac cagagacaac caaatgagag cgactgtcat aattgatgac gcacaggacg
     1621 gaaaaaataa tagctgccgg acagcagcaa ccagcagtcg tccggtaaaa aagacaaaca
     1681 aggcgcattg aggtcagcct tcgcaacaat ccggcggcag atgtcctgcc gccgtgttgc
     1741 ccgttaacgt ttaacttcgt aggctaacgt tgccacaagc accgtatttc cctgctcgat
     1801 acgaaatccg caaagcgaat ccctcaccat ccagacaaaa cacagtatcg tcacacagac
     1861 gacgattaac atcattaaaa acgttcgttg tggcatcgca gcccctccat tgcctttcgg
     1921 ccgtcatcgg gctactataa tgttgtctag gcattacagt gggccctggg ttgattgata
     1981 aaaaaatcgc ctggggcttt ctgctacatg ccgtccacaa aacgctttcg gcatacagcc
     2041 tcaagcgccc gccgctattc taatcgtttt cctgtccgcc acaatccttt tcatgccacc
     2101 acgtattttg tgtcagggac agaaataaag ccggaaagtg actacgtgtg cacacatctg
     2161 ttttccgggc tgcaggcatc gtgttatttt ctgaaatgca aaataataaa aaacaaggac
     2221 acatcatgaa ggatgaaata cacagtacca gcattaaccc ggatgttgtt gagcagtttc
     2281 tggcccttat gaacacacat tgtaaatcct cagaagaggc actcgccatg ctgagtacgc
     2341 tttgttacaa cctgatgatg acatgcggta tccattccat ggacagccag atgctcaatg
     2401 ggcatgggat atccatgcaa cttcagggga ttgatattgc cgaaaggcca ggcaagaata
     2461 ttcactaacc tgaacagggt ctgaaaacgc tgatttttca ggtctctgca ggctaaaact
     2521 gatgatgtaa tgacaacaaa atttctgcgt ttgtacccca ccgatgaaaa agacaaaaac
     2581 tcacaccggg cttctcatca ttaaagataa aacccgacgt gtcagcctct acgaaacgcc
     2641 aacggcatgg tgtatcaggg ggcaggagtg ctacagcaaa agtacaggac gccgctgtgg
     2701 cagccacgac agtctgagca gactgcgcct ggacagcatt aagcctgttg aatgagtggt
     2761 ggcaccatga aagataacaa cacaaccgcc accccagaaa cctggaaaat gcgttttctg
     2821 gccaacggga catccggcac atggttttac actgacgaca ttgcagtcac tgacgcgtgg
     2881 cttcgcgcgc acccggatga tgtggaggtt atccctctct gccggataca gccccgccag
     2941 cattcccatg aacaacatat tgaagaactc cgcgcagccc tggcccggat gctccatatg
     3001 catcatatga tgctggaaac cacacattac cgggaaaata tcaccgatgc ccgttgtatc
     3061 cgtgaaatgc atgaagcgcc attacaggcg ctcaatattc tgtccagata cccgcataaa
     3121 aaggacgagg tgtgacgcat gtggctactg attttctggc tttcatcgcc ctttaatgtc
     3181 tcttccggtg atgcccgtca gcccacactg gcggtgcaca gccaggcctt tcataccgaa
     3241 cgggcctgcc gcgatgcatt gcgcgccatt aaaaaggaga atgttggcga attttctctg
     3301 cggggcgttt gtgtttctga cggaaatacg aaggaggagg cagtgagccc atgacggata
     3361 ttactgacag acatacactt cgccgtatgt cctggtcgga actcatcacg gcaggacagg
     3421 aagcagaatt tcagcatgat tacgagcgtg ccagaatagt gtggtcattc gccttacatg
     3481 ttgcaacaac cacgattaac aaaaatctgg ccatagctca tattcgacgc tgcgatactt
     3541 tgttgcataa atcaaaggct gtacccggaa acaacccgtg tggttccggt cagcgagtct
     3601 gagacctcaa catcccgggt gatgattgtc attccggact gacgcagccg ctgcacatct
     3661 gcggcaatac gttgaatacc aagccagaac agaccatcca gcgcggtgac aattaacccg
     3721 ctttccagcg ccagtttcag ccgtgctgtc ggggctttaa cctgcccggc tatatcagtg
     3781 aaacaacgcg tcgtcatgcc atagctgtcc gcccgccgta ttcgcgtcgc aagactgtaa
     3841 ctgaagctat ccggcacgcc ggataagtca ttctgcagac tgtatacaca tccccgctgc
     3901 cgctcattga atacaacttt tttctggctg agctgccatt cccgccgcac gctgtcaatc
     3961 atctgcggtg tccgggttaa cagcagacga aacggcagtt caaaactggt cacataatca
     4021 acattaagta aggcaatccg cagccgctcc ctggctcctg ctccggtatc acggcattca
     4081 cgcatgacat cttcccactg gcgcatcagt cgcggtgact cattcctggc tctgaactga
     4141 tatttctcaa tctgataatc aatatgttcc tgcataccgt caccggatat ctgttgccgt
     4201 atcatcacgg atatcagcgt actggaattc atgccctgcg tgaagtatca cactgccaaa
     4261 aaaaacacac ctgaacgtta ttatttcgca atattaattg cacctgtttt cataaataaa
     4321 aaggagacga tgaaatgata aaatatatct tttcactcac cattattttt tccgcttctc
     4381 tctgtgccgc acagattcag gggcacgtca tccgcgttct tgatggtgat acgctggaaa
     4441 tattacagga caaaaagcct gtcagggtcc gtctggctaa tattgatgca ccggaaaaga
     4501 aacagccctt tggacgctgg tcagccactc agctgaagga cctggtggcc ggaaagcccg
     4561 taaccgtgca atacgcgcag acagacaggt atggccgtat tcttggccgg gtctataccg
     4621 ctgacggcac agaagcaaat cgctggcagg tccgtaacgg cgctgcctgg gtgtacccgg
     4681 actacaataa tgactatact ctcccgccgc tccagcgtga tgcccgcgcc ctgaagcggg
     4741 gattatgggc agataaaaat ccggtaccac catggacctg gagaaaacag aataagaaat
     4801 aattatttgc tgatatgaag aaaaaaacca tttcaaagaa aataaatggc aaaaatacag
     4861 catggaaaaa attgccattt ttaccgccgc gttattttta atatttgctg tctgctggtc
     4921 gtaataagca gaataacaga aaggtgtaaa aatgaacctg gttgatgcat ttgtcactaa
     4981 agtgatatcc ggaccgtatg aagaatacgg aaaatggtgg gtggatgtag aatatatttc
     5041 ctggggagtt ccgggtaaaa gccggctcat gtttgactca cgggaacagg cacaggaagt
     5101 gcaggaaggt tttcgatttt taacctgagc atattttgtt aaagggcaga aggcggtgac
     5161 cctctgccct taagtacgtt tccgggatta tcagtcaacc agttcccgga tcagttccag
     5221 gagagctatc actgcgttag ccacagcaac aaccgccctg agaacgatga ttaacaggtt
     5281 aatcatccgc ttctccatca atggagaaag aacaccgtgg tttgccctta cattgcctgt
     5341 cgccacgttt tctatagtgt cagttataga cagttcttct ccggccagag caccaaaacc
     5401 agcaccacct gatgttttgg ccaggacttg caacctgttc atcccggtga acgatatgcc
     5461 ggaaagaacg tgcctcaaag ctgacacacc gagtatatac ttatactgtg cttgcagaaa
     5521 tattatttca gccgtattat ttcatagtca gttataggca gttatcttcc ggtcagggct
     5581 accaacttcg atccggaatc gcaaaaaaac cggcctcacc agtcggtttt tttgcgtctg
     5641 tacctcctcc ccggacagca gttctctcac tcccgttgaa tatttcctgt tgaattttac
     5701 gaaaatacgg caaatggatt gatgcgaagg taagtctcca gacaaggcta acttgctgat
     5761 tatacttgtt tcaggcgata tcgctactcc cggaacaata gactgtaacg aacagattgc
     5821 gatcaggtga atataacgat attctggtaa cgttagttga aaagagactt cctccgggcc
     5881 agagccaccc actctccctg ggatcgcaaa aaagccggcc tcaccagtcg gcttttttgt
     5941 gcctgtctct caccaccttc tgctgttcct gtgaaaatgc cctgctgaat tcagccctgc
     6001 ccggtcatta ccggcgattg ttcgcattaa ttccactcag cctcatcccg aaatttatct
     6061 atttatcgat ggaggaatcc tcgtgtctta ccaacactcc cccacaacag aagacccgat
     6121 gaacatatgg tatatcgttg gcaccatatg tctgctcttt gcgcttctca tctggctctt
     6181 tttacccgac atcgtctttg ccacctgcct gatcctgcac acaatgtggg ggatgactga
     6241 ctgggggcct tttcaccatt ttgccgcccc ccgttataac ctgctggcca acaccgccaa
     6301 caacgctgca acgatcactt tcagccagtg gctggatgtc atgtcacaga ccgtggggat
     6361 cctctggctg attctgcttc ccatgacgat cggctttctg tggatgtggt ttcagcatcc
     6421 ggcccagccc cgcttcaccc gcagaccact gaatatccac acgctgccac atctgttttc
     6481 atcactgtca ccggcgatcg cccccgttct ggctgacgga gacagcaaca gactcttcca
     6541 cggacaaaaa cgtcccgagc gccgtgtcgc tctaaccccc gaagcctttg tggagcagaa
     6601 caaccttatc cgcaacatgc agctcgatgt tgcggccacg cgccagtgct ttatggcgca
     6661 actgggccag cctctgacca cctggaaaga catggctcca catgaaaaag cactctttgc
     6721 cgtctttggc ctgcagttct tccttgggga ccggaaatcg gctgtttccc tgatgaataa
     6781 cctcaacctc tcctgcagac tcaaaagcaa gcgggaccag gggcgtttca gcacgccagt
     6841 gtattcactg gccagaaacg cctttatccg ggtcataaaa acagaagacg cacaacagtg
     6901 gctcaggcag caccgttacg ttcgcagcgg tcttgtctgg ctttacgcac atgatttgcg
     6961 cctgacgccc cccaactggc tgtggctcaa gggcgttgac cggacgctct tctatgccct
     7021 tcaccgggca aacaccacaa aaggcttcat cgaaggggcc ggcgtggtgg ccgttgcccg
     7081 ggcagaaaat gaagccagcc gtctcggcct cccctgcccg gaaccctgtg tggaagaggc
     7141 cattgaaggg cttcgacggg acatgctggg gctgggcctg atatgggacg aacctcagcc
     7201 agatcgcgat cgtaaacgtc agatcaggac ccgctggtca ctcacggatg acgtgatacc
     7261 ccgccgccat gataacgacg aagacactga cactggtgaa accaccgaaa ccaccgaaac
     7321 caccgaaaaa cagcatcccg ccgacaagga aaaagaacaa tgaccatcca ctacactgca
     7381 acactgaata ataaacacct gtccctgacc gccctgtacc ctgacggtaa tcgggaggtg
     7441 attgccatcc tgccggtaac agaactgggc gcactgatta accgtaataa caggctgatg
     7501 cacctgtcct tcagttcccc ttttcgcgaa ataaccgaaa cggtgcttgt tgataccgtt
     7561 gatgcagaac gtcccggaca tcacatcggc agaatgctct cagcgattga tgcctgcctg
     7621 gatgccgaat atgccggaaa aggaaaacat ccctcccgga aaaatggctt ctggcggaca
     7681 ctgatgtgtg gctttatcgc cggaattgct ctgccggcga cagttgcagg ttaccactgg
     7741 atgaaggcag agtccccttc cacgctggct taccctacat ctcaggaatg gagagctgaa
     7801 ccagtaaccg acacagagac accacgccca catacggaac atcatgttaa cagcatcccg
     7861 gcccgcccgg ctcccacaca gcaggaacct tcttctgccc ccgccccgac ggcagtgacg
     7921 gaagaacaac acgttcaggc cgctgaggcc cgccgtaatc tggccacagt acttaaacgc
     7981 aacgcagacc gggggatgtt caccattaac ctgtccactg gtcatgaacg cacgctgtac
     8041 gccttccttg accccgcatg catcaactgc cgtctgctgg aacccgcgct taagcaactg
     8101 gcccgggaat tcagcgtggt gatttatccg gtctccgtga ttggcgggga gtccagtaca
     8161 aagcggactg ctccgctgct gtgtgaaccg gactcacaaa aacgggcgac cggatggcat
     8221 cagctgtatt ccgctgacag tggtatggcc cccaccggcg atgcaccggc cgcacaggat
     8281 ggacagtgtc tgaccgccgc acgtgctgcc atcgatgtca ataaccaggc tttccgccag
     8341 ttcggttttg gcggaacccc ctgggtgtta tcagataccg gctggcacct gccgtccggc
     8401 atcctgaaag acgccacttc cgttcgcctc ttcctgaaag caacagatag cgaggagtat
     8461 cacaatgagc cagcaccacg ttaatcctga cctcatacac cgcacggcct ggggaaaccc
     8521 gttgtggaat gcactgcata acctgaacat cactggtctg tgtctggccg gcagtatcat
     8581 tacggcgctg atatggccac tggcccttcc tgtctgcctg ctgttcaccc tggtcacctc
     8641 cgtgatattc atgctgcaac gctggcgatg ccccctgcgt atgcccatga cactcagcct
     8701 tgatgacccc tcccaggaca gaaaggtcag gagaagcctc ttcagcttct ggcccaccct
     8761 gtttcagtat gaagcggatg aaacattccc cgcccggggg attttttatg tcggttaccg
     8821 ccgtatcaac gatatcggtc gtgagctctg gctgagcatg gatgacctca cccggcatat
     8881 catgtttttt gccaccaccg gtggcggtaa aacagaaacc acctttgcct ggctgctcaa
     8941 cccgctctgc tggggaagag gctttacctt tgttgacgga aaggcacaga acgataccac
     9001 gaggacaatc tggtatctct cccgacgttt tggcagggag gatgatatcg aagtcatcaa
     9061 cttcatgaac ggcggaaaat cccgcagtga aatcattcag agcggcgaaa aatcccgccc
     9121 acagtcaaac acctggaacc cgttcgcatt cagcacagaa gccttcaccg ccgaaaccat
     9181 gcagtccatg ctaccccaga acattcaggg gggagaatgg cagtcacggg cgatagccat
     9241 gaacaaggcc ctggtctttg gcacaaaatt ctggtgcgtc cgggaaggta aaaccatgtc
     9301 cctgcagatg ttacgggaac atatgacgct ggaagggatg gcaaaactct attgccgtgg
     9361 ccttgatgac cagtggccgg aagaagctat cgcaccactg cgaaactacc tgcaggatgt
     9421 cccgggattc gatatgtcac tggtgagaac gccgtcagcc tggactgagg agccccgtaa
     9481 acagcatgct tatctctccg gtcagttctc ggaaacgttc acaacctttg ccgaaacctt
     9541 cggggacgtc tttgcggcag atgccggcga catcgatatt cgcgacagta ttcacagcga
     9601 ccgcattctg attgtgatga tccctgcact ggacacatcg gctcatacca cgtcggcact
     9661 cggtcgtatg ttcgtcaccc agcagagcat gctcctcgcc cgtgacctgg gctaccggct
     9721 ggaaggcaca gatgcacaga ccctggaagt caaaaaatac aaaggcagct tcccctatat
     9781 ctgtatcctt gacgaagtcg gtgcctacta cacagaccgc attgctgtgg aggccaccca
     9841 ggtacgttca ctggagtttt ccctgataat gacggcgcag gatcaggagc gaattgaagg
     9901 gcagacctcc gccaccaaca ccgccacact gatgcagaac gccggcacca aattcgccgg
     9961 acgcattgtc agtgacgaca aaacggcaag aacggtgaaa aatgccgccg gtgaagaggc
    10021 acgggccaga atgggcagcc tacagcgcca cgatggtgtg atgggtgagt cctgggtgga
    10081 cggtaaccag atcaccatcc agatggaaag caaaatcgac gtgcaggatt tgatcagact
    10141 gaatgccggt gagttcttta ccgttttcca gggggatgtg gtgccgtcag cctctgtata
    10201 tattccggac agcgagaaaa gttgcgacag tgatccggtg gtcatcaacc gctatatttc
    10261 cgttgaagcc ccccggctgg agcgtctccg ccgtctcgtc ccaagaacag tgcagcgcag
    10321 gctccccaca ccggagcatg tcagcagcat catcggtgta ctgacagcaa aaccatcccg
    10381 taaacgtcgt aaaaaccgta cagagcctta ccgtatcatt gatacattcc agcgccagtt
    10441 ggcaacagcg cagacatcac tggatctgct tccgcaatat gatacagata tcgaatcccg
    10501 ggcaaacgaa ctgtggaaaa aggctgtgca cactatcaat aacacaacct gggaagaacg
    10561 ccgggtatgt tatatcacac tgaatcgtcc ggaagaggaa cactccggtc ctgaagatat
    10621 accttcagtg aaggtgattc tgaataccct gctcccactg gagatactgt taccggtcgc
    10681 tgaaaataat aaatcgcaga caataaataa tgatcatccc cagaaatcat gtggagcaca
    10741 accaacaact gacgaaaaat actgaaggaa tacaaatatt ttctggtgac catatctgtc
    10801 aatcagactg taaaaccctg ttcactaagt ccggggtttt acggcgcact ggataagggc
    10861 cgcagacacg gcccttaatt cagagttaac cgggtataaa ctcaattcag tgactactcc
    10921 cgcccttatt gacgaaggtt cctgccgttt ttgggctgaa tctttgcacc atcacttatc
    10981 cgggcttaca ttccaggtga tggtggctcc catgttttct cctgctgtaa cgtataatta
    11041 tcatcatggc tctccgataa acgggtctgc tgtctgatat attcccggta atactgctca
    11101 ctgcgttgac ggtgcaaatc cacgtccgga cgagtaatgg tgaagtgtcc gtttccggtt
    11161 attcccgctg cattgtgcca ggccaccatt tctgcaaaca catcattaaa ctgctggtat
    11221 gaaccttccg gctcaaaacc aagctttccg ttgcggctga acagaactgg cgcaattaaa
    11281 ttaagcttct ctgccagttc atttcggtcc gtgtgatggt agaacacaac ccgatctccg
    11341 taatccgtac agacattttt tcctgtccgc gtatccctga aatgaacact tccatttttc
    11401 tgaagacgag cttcaagttt tgcaacatta ttaaaaagtg gcgttcctcc gggttcgcag
    11461 agtacgacac aacgatcaac attagttgtg cgtcttttat ccttattacg cgagcgacga
    11521 tcacgataat cccagccacg taactgcgat actgcagccc tgtctccctg agccgcctgt
    11581 tgttccaccc actgccggta ggacaaagga taccacttac cctccgcaat cagtgacaac
    11641 cgctcttcct tcacactctc cttcagcctg atcagcgcct gcatacgctg cacttccgca
    11701 atatggtaat gcagcttacg caattgtgga tcccggaact gcacacggat atacgccttg
    11761 cgccgacggc aggcagcatg aatttcccgt aaccgttctc cgtaacgtaa gtcgggttta
    11821 cgccagtgct ccttccatgc aagataacgc gcacgcaaat cttcccgcgc agctgcacgg
    11881 gcttcacggc gctcacgacg aatctcagga tcgcgccgga agcctggctc cacttcatca
    11941 ctggcagcaa gttccggatt gtaacgacat tcaggcttca cccgttcgaa aatatctgca
    12001 gccgcaattt caaatggccc ggcctgaggc tcggcacggg ataacgtcaa atcaggatgg
    12061 atactgctgg ccttcaccgg tgtcagctca tgattaaacg catccacaat cacaagacca
    12121 tgatgctgtt cctgcagcat aagcccttta cgtgcaaaca tctcatgaca tgcctgccag
    12181 ctgaaatccc cgtcagctat catctcttca attaggctcc tgcactgaat aaggccgtat
    12241 ccccacagac tttccttatc agaaaacagt tcacgcttta tagccccctg cggacctgtg
    12301 cccatcctca tgcgatacca ttcgtaatca ctgacaggct gagagccaat actgtgtccc
    12361 cggaatcgtt ctccgggaac gacctgagta aaaatatcct tcggtacagg ctgccatcca
    12421 ccatccagat cctgcatata atccagcgtc attaacggcc acacactgtc tgcacgtact
    12481 ggtgtctgtt gtttatcaaa tccatcatgc agtaccagat gcccgtgctg aatccccatc
    12541 cataatcccg cctctgccag agtacggtgt acagccaggc aacttttcag ttcaccattt
    12601 cgcgccattt tcggtaagcg ttcagcaaag tgacgacagg cgtactgttt cagactctcc
    12661 gtttctgcca ctttttccgg ccggatatcc acgttaatgg cttccggatc ataacgaccg
    12721 ggtgtcccca cctgactgaa aatatctgtc ggtacaggct gatattcccc cagtttccgc
    12781 cccagttttt cagctgtcca tgacggtccg aacgaactga gtgcaacacc ttcccgcgct
    12841 ctgtcccagc catccttcac caccagttcc ccttcctgca tcgtgatata cagaccatca
    12901 ctggccagcc gtttatgcaa agataaccag tcctgtgcag gctcttcacg cagcccggca
    12961 atcgacatct gagcaatata ttccctgaac gtctgctttt tcccgcgtcg ccaggcatta
    13021 cggcgctcac gtaccagcgc tgttttgcgc acaatacggt tgccgggtgc atgcacaaag
    13081 cagccattat ccggcgcaaa tccgtgctta agttcaagtt cacggcatgc acgactgagt
    13141 ttttcctggc tccacggaag gcaattgata tagccggttt cagggtggac acgattcact
    13201 gcaacatgta catgcaggtt atccgtgtct gtatgaacgg ctgccacata ctgatgtttt
    13261 gacagctcaa gggatttcag ggtgtgacgc acacagtcaa aaagctgctc agaacgcgga
    13321 ctttcgtgcg caggccagga gagaatatag tgaaaaacag ggtctgtatc atctttggaa
    13381 aaatgagcaa gctctgctgc tttctgcatt tcttcagccg cagattcaag agacaaacag
    13441 ttatgaaagc aggtcacacc atagaaattc acccactgac tgccgtcttt catcacatca
    13501 atcagggaaa caaatttttc attacgcaga cgtgttgcat aatcaacaag acgtctgaag
    13561 cggttccggt gcggaatctc agctttcatt tccgtgtcag gcctgagttc atcttccggc
    13621 acatcatccc gcactgaggt gtacgcaatc aggtcttcaa aagaagactt tccgtcacgt
    13681 ctttttttcg gaatgatggc attcatcagc gattatctca gcgtcccatt aaatttcttc
    13741 gcagagtatt agcaagctct gtaatagcaa cgagaacctc tgcatactct ttatctcccg
    13801 tgcgttttcc ttccacaaac agatgcttct gcaggcgacc aagacgcatc agctctttca
    13861 aaaaatcttc gtcaatgcgg gcatttatct gttttcccag cacagttttt cttatcagag
    13921 ttgaaactgt cagtccgttt ttttctgcct tttctttaac aatctcagcc tcttctggtg
    13981 aaaaacgaac agcaatgagg acattctttt gccgtttctc actgccactt cgggttttct
    14041 tctcactcat ggtctgctcc ttaccggggt gtcggggcta agccctgacc aggtggtaat
    14101 cgtatagccg tgcgtgcgcg gttatacgat tacacatcct gtcccgtttt tcagtccatt
    14161 atagcctgtt atttttgtgc atcatagtct tatgcagacg gcagaggcag ggcgcagccc
    14221 tgccaggagt cagtttttga tgataaaatc aggcttcaac attaaacgga gagaaagtgc
    14281 caatggcgtc taaaaaattt tactcagatg atgacatcca gctggcaaaa gctgctttat
    14341 cggaattgcc agacctcacc gctcaacgga aaacattgcg tgattttctt gatgccattc
    14401 gtgatgacat tattgtcctg gtaaggacaa agggttatac actggcagat gttcgtgaca
    14461 cgctcaaaaa tgcagggtat gaagtaggag agaaggcact tcgtgacatt attcgggaag
    14521 cagaatcgaa aaaactatcc cgccgttctt cctctaaaac agcacctaaa aagacagatt
    14581 caggcagtaa agatggcatc gatatgaaca acaattgatg tgacaatgtc acatcgtgtt
    14641 ctgagattat gatgattaca gaaaaaaaag ccccggcata aataccaggg cttttctgtt
    14701 taatcagact cgtggcggca atgcaccagg ataatgccgt gcaataatat cattcatttt
    14761 ttcaacaagg tcaggacgct taaagacaat gtgtacggtc cctttctggt agccacgcac
    14821 cgtaaaatat tccccttcta atagcttacc gacattgtcg ccgttcccga gaaattcacc
    14881 atactgcgcc ccgatgctgt tcctgaagtc aggaacgttg cgcgcctcca gcagataaaa
    14941 cggacgtacc aggtcatcca gtgcctcccg tccgctgcag tccagcgtca cacgtccgtt
    15001 actccagcgg tacagcaggt tttcaataat gattttctta cccagacggc agggattatt
    15061 ggttctgtaa tcccatgaca atttccggta gacatcaatc agtccctgtt caaatgtctg
    15121 catcttgctg gcattcagat ggcggaatgt tgccagtaca ttgtccaggg tgatttcagg
    15181 acaggtgtcg ctcatcagct ggctgttcca ctcatcacgc tggcatgaac tcatgaaggt
    15241 gtacatgcca gtatccgtca tcaggcgatc ccagattttc cggtcggcaa gacgggtgat
    15301 attctttttt gcttcttccg gtttatccat gcatcgaacg gagtcagtca cgcagtcttc
    15361 aaacccgtaa agaaaaatat cacctgacgc atcgagcaga atcttgcgcg ctgtcgccag
    15421 gcattccatc gcctgcatga acagtgcaat accttcgtta cgctgcgcga tgatgcggtc
    15481 gatagccaca gacggaataa catctgtcgt gccggcagta ttcaggtcag cagtgggtaa
    15541 agtctgattc atcacattgt ctcctcatcg tcacgactgc cgggtcagcc aggtcatttc
    15601 ccggaggagc gaagctcctt ctgccgtccg ggtcttcggg gatttccccg cagtggacct
    15661 ttccgacggg ggacgctcac cacgtcacgg ggcagttgtc cgggggcaga cgcaaaagtt
    15721 tttgacgcct gcggcaaaaa gttttgtggc tggtacatct ctcccctgga cgactgaaac
    15781 gggatgtggt c
//
LOCUS       DAZOZD010000085        15701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOZD010000085 DAZOZD010000000
VERSION     DAZOZD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.085"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(111..329)
                     /locus_tag="ACHUPB_002168"
     CDS             complement(111..329)
                     /locus_tag="ACHUPB_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758978.1"
                     /translation="MTYQKSHHLPFDYSLFPPALTLITALTLITAALIIVCFVVLLLM
                     PFPGVPLAGRAVVNGASEAVSALAGFHP"
     gene            complement(552..677)
                     /locus_tag="ACHUPB_002169"
     CDS             complement(552..677)
                     /locus_tag="ACHUPB_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR0758979.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(619..768)
                     /gene="mok"
                     /locus_tag="ACHUPB_002170"
                     /pseudo
     CDS             complement(619..768)
                     /gene="mok"
                     /locus_tag="ACHUPB_002170"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(947..1709)
                     /locus_tag="ACHUPB_002171"
                     /pseudo
     CDS             complement(947..1709)
                     /locus_tag="ACHUPB_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(1706..2140)
                     /gene="psiB"
                     /locus_tag="ACHUPB_002172"
     CDS             complement(1706..2140)
                     /gene="psiB"
                     /locus_tag="ACHUPB_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0758980.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(2195..4153)
                     /locus_tag="ACHUPB_002173"
     CDS             complement(2195..4153)
                     /locus_tag="ACHUPB_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0758981.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(4217..4450)
                     /locus_tag="ACHUPB_002174"
     CDS             complement(4217..4450)
                     /locus_tag="ACHUPB_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR0758982.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(4506..5027)
                     /locus_tag="ACHUPB_002175"
     CDS             complement(4506..5027)
                     /locus_tag="ACHUPB_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0758983.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            5326..5769
                     /locus_tag="ACHUPB_002176"
                     /pseudo
     CDS             5326..5769
                     /locus_tag="ACHUPB_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5855..6418)
                     /locus_tag="ACHUPB_002177"
     CDS             complement(5855..6418)
                     /locus_tag="ACHUPB_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0758984.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(6465..7826)
                     /locus_tag="ACHUPB_002178"
     CDS             complement(6465..7826)
                     /locus_tag="ACHUPB_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR0758985.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(7878..8108)
                     /locus_tag="ACHUPB_002179"
     CDS             complement(7878..8108)
                     /locus_tag="ACHUPB_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758986.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(8332..8451)
                     /locus_tag="ACHUPB_002180"
                     /pseudo
     CDS             complement(8332..8451)
                     /locus_tag="ACHUPB_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(9146..9337)
                     /locus_tag="ACHUPB_002181"
     CDS             complement(9146..9337)
                     /locus_tag="ACHUPB_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758987.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(9334..9756)
                     /locus_tag="ACHUPB_002182"
     CDS             complement(9334..9756)
                     /locus_tag="ACHUPB_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0758988.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(9803..10228)
                     /locus_tag="ACHUPB_002183"
     CDS             complement(9803..10228)
                     /locus_tag="ACHUPB_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0758989.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(10646..11422)
                     /locus_tag="ACHUPB_002184"
     CDS             complement(10646..11422)
                     /locus_tag="ACHUPB_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758990.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(11473..11907)
                     /locus_tag="ACHUPB_002185"
     CDS             complement(11473..11907)
                     /locus_tag="ACHUPB_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0758991.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(11921..12142)
                     /locus_tag="ACHUPB_002186"
     CDS             complement(11921..12142)
                     /locus_tag="ACHUPB_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758992.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(12143..12826)
                     /locus_tag="ACHUPB_002187"
     CDS             complement(12143..12826)
                     /locus_tag="ACHUPB_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0758993.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(12903..13196)
                     /locus_tag="ACHUPB_002188"
     CDS             complement(12903..13196)
                     /locus_tag="ACHUPB_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758994.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            13343..14305
                     /gene="parM"
                     /locus_tag="ACHUPB_002189"
                     /gene_synonym="stbA"
     CDS             13343..14305
                     /gene="parM"
                     /locus_tag="ACHUPB_002189"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0758995.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            14308..14658
                     /locus_tag="ACHUPB_002190"
     CDS             14308..14658
                     /locus_tag="ACHUPB_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0758996.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(14781..15062)
                     /locus_tag="ACHUPB_002191"
     CDS             complement(14781..15062)
                     /locus_tag="ACHUPB_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758997.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(15190..15424)
                     /locus_tag="ACHUPB_002192"
                     /pseudo
     CDS             complement(15190..15424)
                     /locus_tag="ACHUPB_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(15435..>15701)
                     /locus_tag="ACHUPB_002193"
     CDS             complement(15435..>15701)
                     /locus_tag="ACHUPB_002193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0758998.1"
                     /translation="RNNGNGCANLYLEVLLQGTSTPSLHQYRIAPDTRHPDINLIKAH
                     LDEGFQQAKSEGLKVEISDYKERLYLYIRTPGNNLMQYSGCREK"
BASE COUNT         3498 a         4543 c         4110 g         3550 t
ORIGIN      
        1 ggcgttgctc cttacatccc ggcgtctttt gccgggatgc cgggagcgca ggccacggcg
       61 cgacaagggt ggcgcgaccg ttccggtcgc tgcaaacccg ggtttttgcg tcagggatgg
      121 aagcccgcca gggcggagac agcttcgctg gctccgttca cgacagcccg ccccgccagg
      181 gggacgcccg gaaaaggcat aagcaggaga acaacaaaac agacaataat cagggcagca
      241 gtaatcagag tcagagcagt aatcagagtc agagcaggag gaaaaagaga ataatcaaag
      301 ggaaggtgat gtgatttctg gtaagtcata aacagagaag gcgggggaaa agaacgggcg
      361 ccgcaggcgc acgttcatcc ccgttcccgg agggaaacgg ccttcctggc agtctggttg
      421 ttcatgcccg tacaatgcca gcaaggagaa aggggccacc ggcgaaccag cagccccttt
      481 ataaaggcgc ttcagtgcag tcagaccagc atcagccctg aaaaggcggg cctgcgcccg
      541 cctccaggtt gctacttacc ggattcgtaa gccatgaaag ccgccacctc cctgtgtccg
      601 tctctgtaac gaatctcgca cagcgatttt cgtgtcagat aagtgaatat caacagtgtg
      661 agacacacga tcaacacaca ccagacaagg gaacttcgtg gtagtttcat ggccttcttc
      721 tccttgcgca aagcgcggta agaggctatc ctgatgtgga ctagacatag ggatgcctcg
      781 tggtggttaa tgaaaattaa cttactacgg ggctatcttc tttctgccac acaacacggt
      841 aacaaaccac cttcacgtca tgaggcagaa agcctcaagc gccgggcaca tcatagccca
      901 tatccctgca cgctgaccac actcactttt cctgaaaata acccgctcat tcaggctgtt
      961 tacgggtaat ctgtgtgatt gttgccgcat cacgccgcct cccggagttt gttcggcacc
     1021 atccaccgct ccatctcacg gacagcgtga ccggcctctc ttgctgcaag actggcttcc
     1081 gcaataacct gccagaacgg catatcctgc cactgccagc gttccattcc ggccagtgac
     1141 ggaaaacgct caccccactg ccagaacagg gtttccaggt catgctctgc cacccggtca
     1201 ctccagcgtg cataccagct tccgactgtt tccggcgaat ggaatgccag ttcgatttca
     1261 gcctgagcga ccagcgcctg gtacgcacgg gctttctgct ctgcctccct cgcctgctgg
     1321 cggctgtact tctgcatgac cagcgcggat ttatggcgac tgcgttccct gacacgaaac
     1381 cgcaccgacg ggaacaagcg ttctgctgca tcgcctggaa gaggaaggag gcagacctcg
     1441 ccctgtgttt caatcagcac atcgatggca tacagccact gaagacggct gccgtgcagt
     1501 tcttctgcgg tcagggacgg catgaaaaat cgcaggtcag acagcgatat cctgcggctt
     1561 ccgttcagac agcggaaaaa cgcccccgca tacggatatt ccgccagcgt gtttccctgg
     1621 tggcggcgtt tttctgtctc cgccaccgcc cgccatgcgg cctgttgctc tgcgctgagg
     1681 ggaaccagtg cctgtgaacg tgcgctcatg cctgacctcc ttccgccatc aggatatgga
     1741 tgatgtcact gacgctgtac ccctgcgcgt ccagtgacgc cgccagtgcc agtgaatggc
     1801 tgacggcttc cggtgcaaac cgccgctgta cctgaaccac cgcaaacggc tcaccaccgg
     1861 cattcaccag aaccagcacc cagacctgag agacatcgcc cggtgaacat aacgccaggt
     1921 gaaaacgttc gtgggcaggc gtgaaacgga cctggacggg gaaaaatccg ccaaactcgc
     1981 tgccgtactc gccgttcatc gtccagttgt ccggcgcatc cagttcccgc atcacggcgt
     2041 gtgtcagctc acgacgttca tcacttccgg cagcccggat atcttcatat tcctgtgcgc
     2101 tcatggtctg taatacattc agggtcagtt cagttttcat cacggtatct cccttgctgc
     2161 cgccccgtct ccggcggggc ggtgtggttg ttcatcaggc ggcatcagcc aggttatcgg
     2221 tgcgttcagt ggtatctgtc tgtggatgtg gtgcacacat ccagccaggc acccagcggt
     2281 tgtctttcat atggtgctct gcatgttcag ccgcatcacc tttcttcatc ttctccgcgt
     2341 cccgtgcggc accggacagc cctgcctcat tcagggctgc gataatctgc ggctttttca
     2401 ggtgtccgaa gaagtttgct tttgtcggct gccaccagtc gcgcatgtga aagccgatgg
     2461 cgctttccag cgtgtcaagc ggactgcgtg acgtgtgacc acactcacgg gtctggacac
     2521 cgttaaggct gcatgcggtg cagaaactga gcagggataa cagcacctcc tgactgagtg
     2581 acaggaatgt cgtcatgtcc cgtgaccatc cctccggtaa cagggcagca agacgggctt
     2641 tttctgccat cagcgccatg aatgcggcac cttccttccc cgatggcgca tcgctggtca
     2701 gcgaataatg tttacattcc aggctgattt gtgctggttt actgtacgct ccgctgccaa
     2761 acacattcag gcagagcgtc catgccagca gtgtcaggga tttgtccggt tgctgcatca
     2821 gtgctgcctg gactgccagc gtgcgttcgg aagacatttt cgtcagcagt ggcagactga
     2881 tttcctccac tgacgctttc tcctgcacct gttccgtacg gtcagcgtcg tcagccacgt
     2941 catcctcact gcgcaactgc acaccacgct ggacacacac gttgccataa cgccaggaca
     3001 ccaccactcc acctccggca cgcatctccg gcgtccacgc cctgaccttc gccatgcagc
     3061 gcatcagctt catttctgct tccagcaggt cagattcctc acactggttt tccagcgcgt
     3121 cgtaacgcgt catcagttcg ttcagacgtt cttcttccgc ctccgtcagc accgcttccg
     3181 gctccggcag acagcggtat gttccggcat cctcacggca ctcaccgaca ggctccatgc
     3241 gtccggcgca ccattcccag ccttcggctt cccgaaggaa ctctgcgaca gcctggagtt
     3301 tttccagcag ggcggcatcg agcgccacgc agtccacata gccaccttcg tcgtcgctga
     3361 acaaatcggt gcgcagttcg tctggcgaga aggcatcagc ccccacgaag cggaatttac
     3421 tgttccccgc caccgccact tcactttcgg tcaccagacg acgaatggtc tgtacttccg
     3481 gtttaccgcc ccatcccgac tgacaggcgg cttcaaacac ctgcacctga cgcgcggtgt
     3541 cgttctccag cgccagcgcc tgacagtgtt cggtggtgat gcggtcttct gccagcgcat
     3601 cgaggatgac aggcgcaagg tcagccagtt tcagcattcg ctgaacgtgg cggggtgaat
     3661 agcccagcaa atcaccgatt tgtgcaggtg ttttgccttc ctgcgccatt gcgcggaatc
     3721 cggcaatctg ttcggcaggg tgcatatccc gacgctgacc gttttcagtc attgatgcag
     3781 ccgtcgccag ctcctgcgga ataaccttca cgcgtacagg ccagtcagcc ggaaggatgc
     3841 cacgctctgc cagcatgttg agtgctgcca gtcggcgacc acctgcggcg acaccgtggc
     3901 ggtcaccagg cagggcatga acaaccagat tctgcaacag gccaacgccc ttaatggact
     3961 ccgccagttc gctgacggac tctgcggaat acggcaccgt gcgcacattc agcggtgatt
     4021 taatcagcga ggccagcgac acgctcactt cctccgtctg ctccagcagg gcagacagga
     4081 ctgtttcctg cgtttttgca ggtttacggc tggatttacg ctccgttttt gtcttagact
     4141 ctgtaactga catatatttc tcctttgacc gggatggttg tatgtctgcc ggacgcgttc
     4201 ccgtccggca cctctcttac ttaagaatgc cgaaattccg gatgacatgg ttcatccagt
     4261 acccggcacc gtcctcaaaa ttccaggtac gccagaccag gccactgtta ttgcggacaa
     4321 ccaggcgaaa atgcgttccc tgatcatcct cgataaagac attccggtac tgtgcggcaa
     4381 cggcttcagc ctgttcgcgg gtaaaggagc cgtcaggcag cccctgaaat attctgaaat
     4441 attcgctcat cctcatatct ccggtgatgc cccgcactgg acggggcggt tgtcactgtc
     4501 tttcgttaga acggaatgtc gtcatcgaag tcatacgcgg gttcagacgg ctgctgaggc
     4561 tccggctgcg cggccttacg tccacggcct tttgttttcg cgctcccttt tttcgtcgtg
     4621 tcagcactct gtggctgacc attctgctga ggctgcggtt gcgcctgagc gttctgctgt
     4681 ggtgcactcc ccagcatctg catggtgccc gtggttttca caaggatttc agtgacgtaa
     4741 cgggtaatac ctttatcttc ccagctacgg gttcggagtt gcccctcgat gtagacctgc
     4801 gcgcccttgc gcagatattc acctgccact tccgcgagct tgccgaacag caccacgcga
     4861 tgccattctg tctgctcccg catctccccc gtctgtttgt cacgccagct ttctgacgtg
     4921 gccacctgca ggtttgccac tgcgcccccg ttggggatgt aacggacttc cggatctttt
     4981 cccagacgac cgacaagaat gaccttgtta atgccacgaa ctgccatgat gtgtctcctt
     5041 ctgttgatgt tttcagcccc catcatctgt tgagtttcgg gagccgcggt tttgacgttt
     5101 tgcggggacc tgctctttgt tccctccggt attcaccatc acctgtttga gaaccttcgc
     5161 cgcatggggc atcactgcca cggcctgacg cacctgtcct gggcagaacg caaaattttt
     5221 taccgggttt tgggtaaaaa attttgtggg ctgttcattt accctggaca ggtgcggcag
     5281 gttgtggctg tctatgcccc cggcgacagg ggcttaaaca ggtgaatggc accggacaga
     5341 acaacgggca gggacccgcc gcaacgacgt gaagcgcggt ttgcgaaacg gcgttgctcc
     5401 ttacatcccg gcgtcttttg ccgggatgcc gggagcgcag gccacggcgc gacaagggtg
     5461 gcgcgaccgt tccggtcgct gcaaacccgg gtttttgcgt cagggatgga agcccgccag
     5521 ggcggagaca gctccgctgg ctccgttcac gacagcccgc cccgccaggg ggacgcccgg
     5581 aaaaggcata agcaggagaa caacaaaaca gacagtaatc agagcagcag aaaaaagaag
     5641 aacagtagca gtagcagtag cagtagcagt agcaggaaca acaacatcat cagtaacagg
     5701 caaatgacag gtctgaaaag acagaagccc acagcagaac gtccgcgtaa tgaaaggggc
     5761 ggcgcgtaag cgcggcccct ttcagcgcca ccggcgcgcg gtcttttccc ggcgcgggca
     5821 gacgggcatt tatcctcccg cctgcccgga ttcatcaggc acgcagacga ataatcatcg
     5881 tcggaacacg ggtataagca aatgtgccgc acggcagcac ctcgcggaca tcagaaaacg
     5941 gtaacagctt ctcctgctgg cgcggcccgt tcagacagac ggcaaccaga acgccccccg
     6001 gacgcaacag ggaaaaagcc cgcaggatat gccggatatc ctgcccgtga ctgaacggcg
     6061 gattcataat gatccggctg taatactgca ccggctgcca ttccatgaag tcgccacact
     6121 gtaccctgac accgttaaag ttttcccgca gataccggac cagcccgctg ttaatctcca
     6181 ccgcatcaca catggcctcc ggcgcggtat cccgaatcgc ccgcagtatg gccccggtgc
     6241 cggcagaggg ttccagaatg tgatcacggt tgctgatttc cgccagcgtc accaggcgat
     6301 cacagaccgg ggcaggagtc acaaaaagct gagattcagc ccggatttca gcaaccggcg
     6361 cggcgttatc cagccttgcg cgcatcagcc ggatacgttc tcttaactga ctgtgcatgt
     6421 cattatctcc gtcatccccc ggggatttcc cggggggata taccttattt ctgcgggatt
     6481 tccgtgattt tcatgtcggt gatatacaca ttcaccagac ggaaattatt gtccattgtg
     6541 cggcggtagc ggtatgcccc gtggtcttct gcttcttcca cactgcgcac ggccttacag
     6601 tcccggggca gggcggccca ctgcgccttt gtcatttccc ggaaaccttc ccccggatag
     6661 ttgactaccg gcggacgctt cgcggcctga ctggcgacgg cagcctcttc tgccgatggt
     6721 gctttgtagt ccgtgatgcg atcgggcgtc actttcatcg tgccgctgta cccgagaaaa
     6781 ctgtaatttg gcgttgtgac tgaactcacc gccccgttgc ttttgttcac gcggatgata
     6841 gtcagccact cgccccggct gaaaacctgt ccgcccggct caaaatcctg tgtccgggta
     6901 accacgccgc cgctttcgtc cagcatggca cgctcataga tcaggcggtt ctgatagtgg
     6961 ttaacccagc gttgctgatg ctgaatctgc cgttcatgac agcggatcgc aatttcgcgg
     7021 gcctgctccg tggtgatgat gtcatcatcc agggccgacc ataacgaccg gctcccttca
     7081 tactggcttt tttctgccgg gcgcggatac gtatccagcg ggaaacaggc gctgatatgg
     7141 tcatgactgc tgatgagttt tgccatattc agatccagcg attcagcccg ccacatcgtc
     7201 agatatttct gcgactgtgc gatggtcttt tcagccttgc gcaaatccgc ttcgattttt
     7261 ttgatacgac gccagcgaac gtccgggcgt tctttatact tcgcgtgaag cagtgccgac
     7321 cgcgcccgct cctcccagta ttccgcacgt tcaaagagca tcacggcacg tttcatgccg
     7381 ttttcaatac gctgcgcatc acgacgggcg cggcgttcgc tgtgatgccc cacaagaatg
     7441 ggctgaccgg gcgggatcat cgcggccagt ctttccactt catcaagtgc ctgtgcggat
     7501 tcactggccc gctttccgct gtatccggta aaccgctccg cccgtgcttc ctgacgttca
     7561 gcgagcgtgc tgtcttcatc ctcaatctct ccggcaagtg agaggagcac gtcttcccgg
     7621 cccggtgtcc aggccggcgc gacaaacagc gcctgtttgg gtgcccagcg aaaaccggca
     7681 tcatgcactt ttttatacgt ctccgggtca agacgtgacg cggcatacag gcgcagtttg
     7741 ttgtcatccg gtgagtaggt cgcgcggtac atctgcacag ggctggcggc ttccggggcg
     7801 gtcatggtga ttgctgctaa tgtcataaaa tgttctcctt ccgtctgttg ccgcccctcg
     7861 cggggcggct cacctcatca gttaaaagcg cgtttcagtt cgagtgtgcg gagcacctgc
     7921 ccgaaagccg tgtttttcat ggcggggatg tgtacaccac gcccccggtg aaaactccgg
     7981 gctatgtgtc gcccgaaggc tttcaacacg gggtcaattt ccggcgtggg gatgtcttcg
     8041 cgtctgaaac ggggatgacg gcggggcttt acagccgctg cggaaggtga aacaatgctt
     8101 acagtcataa atggattcct tatgacaaat tggttaacaa tgccgtcaaa attggtcgca
     8161 caattcttac ggcgtggatt aagcaggtgc ctgacctgct tttttcatat ctgcatctga
     8221 cgctttccgg tgttttcctc cttctgttcc tgcgttctca ttacctgtca gccggatgac
     8281 tgacagaaca aataaatgcg tattgccgga gcctgaaata aatcctgatt tttaaagaac
     8341 atcacccgtt gagtttcggg agccgcggtt ttgacgtttc gcgggaacct gctgctgtat
     8401 tcactgtggt atgaacttca cccgtttgag aaccttcgcc gcaaggggca tcactgccac
     8461 ggcctgacgc acctgtcctg ggcagaacgc aaaatttttt accgggtttt gggtaaaaaa
     8521 ttttgtgggc tgttcattta ccctggacag tcagtgcggc aggttgtggc tgtatcagcc
     8581 cccggcgaca gggactcata cgggtgtgtg gcacccggca ggcaatgagc aggggcccgc
     8641 cgcaacgacg gagagcgcgt gcgcgaaacg gcgttgcccc ttacatcccg gcggcctttt
     8701 gccgggatgc cgggagcgca ggccacggcg cgacagaggc ggcgcgaccg gaacggtcgc
     8761 tgcaagccag gggttttcac gcatcggacc gtagcggcag cccgaaaccg cgccagcggg
     8821 ttcggcggag actggcaggt gcgccagcag ctgcctgtcg gagtcgggcc tgacgaccgg
     8881 atgccgccag gcagccggga gccgaaggtg cgcccggcag acagaacaac actgcagtag
     8941 tcagaatcag agcagtagat aaaggaacaa caacatcagg aacagttcca taacataaga
     9001 aagaaacact cctgagtcag acacatcaca gacgggaagg gtaacgggcg acgcgccagc
     9061 gtggcccgct tcccttccgg caggcgtcag cctgccaggg gttttcgcgc gggctgacag
     9121 gcggcctcca ccgcctgccc ctgtatcacc tgtttatgct gccctgaagg atgtagtgac
     9181 cgccgaggaa tacagcctgc tgattcgcca caaaatcgca gatttcatca acaatctgcg
     9241 cagtggtgag cggtttctgc cccatttcac gacgccgcgc attaatcacg gctttgtaat
     9301 tgcgcagaat ttcgcgggtt tctgtgctga tattcatgac atgtgctccc cgtccagcgc
     9361 ctgtaacaca acgtccattg cttcgcgttc ttccctgacg aatgcgtcac cgtcgccccc
     9421 gttctcgctc ccgacggcat acaggcgctt catttcactg cctgccagcg ccatcacttt
     9481 ttccagcatc accgccggaa ctgtcacctg acggctggcg cgcttttctt ccatcagctc
     9541 acgaacaacg tcgtgaacaa tgctgacatc cgcaccatgc gcaaaggcat cgtccagtcg
     9601 ctgcatgacg gtttccagct catcgtctgt cagactccag tcctgggcta tgtgacgcac
     9661 atccccacgg gtcagcacca cggcaaaaac gcattcactg tccggcacgt ctgtgttcag
     9721 cacttcgcgg ataatttctt taacagtaca gtacatgttc tcgtctcctt tacaggggcg
     9781 gcattgccgc cccgtccgtt aatcagtcca gtgccgcgta aatcgcgttg cattcggggt
     9841 gaaattcgat gtgacgccac aactgcgcat cgcgcatccg gtaaagctgg gtcagtcctg
     9901 catccccgct gtcgtggtac agccacaact ggcggttaat cacaagggaa gtcagaatga
     9961 tgcctgcggc atcagcactc acggtacggt caaaccagtt tgcaccgttc accatgtgga
    10021 aacggtcgcc atccggcatc atgtaaccgc caccgtccgg caggcggatg aagtgccaga
    10081 agcctccctg atattccggc atcatttttt gcgccagcgc gtacacatac gcctcggcat
    10141 acagaaaatc gttgccaaac agatacggta gaaaggagag acggtcgcac tcttcaacgt
    10201 tcagagtgac aggtttcgca tattgcatga tgttttcctc cgttgttcac atattccgcg
    10261 acaccgggcg gtgccgtgcc tccataaaag ccgggcgata aggcggggat gcacagggcg
    10321 caagggaagc cgcagcctga cgcacacggc tggcgggagt tggagggtga gaccgcgaaa
    10381 tctgagcggg tgaacgaccg gaaaatcacg gcagacggga aggagggacc gggtgaccgc
    10441 agcggcgaag cgtacccttg catccctgcc agcccggcta agatggaggc acggcacagc
    10501 ccgcacacga cgctgaggcc acataactga aaaaccacaa ggccatgccc tgcatggccg
    10561 ggtaaagcgg agccggcggc gacttccttt gtgctctgtc cggggaccgg caggaaaaag
    10621 gcggggattt accccgccaa cgtcatcatt tccggatttt tgccagggca tccgccatgc
    10681 tgcggatttc ggcccgggct tcccggacca cctcccgcag aacaccacgg tcaaaccagc
    10741 ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg gacaggatat
    10801 gctcaataca atcgctgacc aggtacattt ccaccgacga aaaacgtgct ccccggttca
    10861 cgatggcatc gagcaccacg ggcaggtttt cgtcatcttc atcgagccag caggggtgat
    10921 cgctttcctt cacaaccaga cgaaagcggc gcgtctggcg agtcagcaac gtctgaccgg
    10981 tggtggtcag cgccgcccgg atacgccgcg catcttctgc cacccggcaa ctgacactgc
    11041 gtaccgcatc gcgcaccgcc gcaagtacag ccctgatttc gtcttcggtc agccattctc
    11101 cggcatacgg gaaagacacc tgcgttccgt ctgccggaag taccggcagc gactgccact
    11161 gactgccgga cagtaccagt tccgccccgg ttccggtcag tttctgaatg gcggaggcca
    11221 ggagcggcgg cagtacgccg ccgtgacggt tgataagtaa cgtcgccagc cggggcgcat
    11281 ccagcagaac ataaatgctg atccagcccg gatacttcgg gttacgctgg tcaacatccg
    11341 tctcttccgg ccagccctgc gccagcagca ggttgcgggc gtgacgacaa atcagtgcgt
    11401 tcagcgtgct gttttcgttc atgattaagc gttctccctg tatcgccgcc cgcagggcag
    11461 gcggcgataa catcagtcgt tttcgtgtgt gttgtttttc agcagggtgc gggcctgatt
    11521 aaccacctcc tgacggcggg tgacgcattc cgggacggcg aggccatgat cccgggcggc
    11581 ccattcacgt ttccacaatg cctgttcagc ggtctgaatc agggacgcca gcagttcagc
    11641 agggacggtc accaggcggt tttcgctctc gttgcggata atctccatca ccgcagcgga
    11701 agaaataccg gactcaatcc gctggtcttc cgggatgtcc tccaggcgtg ccaggacggt
    11761 cctgatttca tgaccggtca gagaaatttc cataccgtca gccagtgcct gaatctcttc
    11821 cggggaccag ataaccagca tcagcggcat gtctcccgga tactttactg ccagctcccg
    11881 gcatagcgtt tcgcatgttc cgtacattct cgtctccttc tcaatcttta aagcacgctt
    11941 ccagttccag aatttcggta tatgcctcca tcaggaggcg gtttgcgcga aacagcgtct
    12001 gacgcaccag acgttccggc agggtgtcgg cattaaagct gtaccgctgc tccatctcat
    12061 tcatggttgt acgcaggtcg cacatggcat tggccacctc cgccacttcg gcgcgcagtt
    12121 tttcgtgtcc tgcatagttc atttacgcag cctccggcat aaaccagtca tcattcgcgg
    12181 ccccctgctg catggcccgt tgcacggcgg caaggcgttg ctgcccggca cggtgatact
    12241 gctcaagcag ctcgataccg atataccggc gtccggactg gagggcggcg acgcaggttg
    12301 agccgctgcc tgcaaacggg tccagcacaa ttgcgttcgg gtgtgtgaag ctctcaatca
    12361 gcggttgcag gctggtaacg ggcttttccg tcgggtgatg gcgattgccc gaatatttcc
    12421 agcccagcac gtccggcagc gggttttgtg gcagacgtgg acggcctttt gccaggatgt
    12481 aggcacattc gtggcgatag cccacatatg cggccttcga tgtgtagttt ttggtgaaga
    12541 ccaggtgacc aacaacgctg aatcccgcat ttttccaggc ggccataaag cgatcgacgc
    12601 ggttccagcc atagaagctc accattaacg cgtctttttt gagtacgcgg tacatttcat
    12661 tacaggctgg ttgcagccat tcatcggttt tatcgccggc gatggtgcgc ccggaacggt
    12721 cacggaaacc gacgagatac ggcgggtcgg tgaggatgaa atcgacagca ttgcccggaa
    12781 acgtggccat aacacgcacg cagtcaccca ggataaaacg ggacataaca gcactccttc
    12841 tcttacagag ggcaccggga gggcacccct gtggtgcccg ttccccgctg cctgtgggct
    12901 gttcaccgcg tccggtaacg ggtcaggccg accgtggaat accgcagccg tcgtcttttt
    12961 gacggcgagg atatgccgcg aaagcggctt gaagggcgtt catcagaaaa gggcgacggg
    13021 ctggcgtaca ctgtcaccaa cgccagccgg gagcggtaaa agcgcccgtc ccgtgcccgg
    13081 ccggacgcgg gacactgacc acaggcagcg ttcctgccgc gccgcgtcag ccggcgcagt
    13141 aaaaagagga gcccggcgac atcttttgtt acccgccaaa caaaacccaa aaacaaccca
    13201 tacccaaccc aataaaacac caaaacaaga caaataatca ttgattgatg gttgaaatgg
    13261 ggtaaacttg acaaacaaac ccacttaaaa cccaaaacat acccaaacac acaccaaaaa
    13321 aacaccataa ggagttttat aaatgttggt attcattgat gacggttcaa caaacatcaa
    13381 actacagtgg caggaaagcg acggaacaat taaacagcac attagcccga acagcttcaa
    13441 acgcgagtgg gcagtctctt ttggtgataa aaaggtcttt aactacacac tgaacggcga
    13501 acagtattca tttgatccaa tcagcccgga tgctgtagtc acaaccaata tcgcatggca
    13561 atacagcgac gttaatgtcg ttgcagtgca tcacgcctta ctgaccagtg gtctgccggt
    13621 aagcgaagtg gatattgttt gcacacttcc tctgacagag tattacgaca gaaataacca
    13681 acccaatacg gaaaatattg agcgtaagaa agcaaacttc cggaaaaaaa ttacattaaa
    13741 tggcggggat acattcacaa taaaagatgt aaaagtcatg cctgaatcta taccggcagg
    13801 ttatgaagtt ctacaagaac tggatgagtt agattcttta ttaattatag atctcggggg
    13861 caccacatta gatatttctc aggtaatggg gaaattatcg gggatcagta aaatatacgg
    13921 agactcatct cttggtgtct ctctggttac atctgcagta aaagatgccc tttctcttgc
    13981 gagaacaaaa ggaagtagct atcttgctga cgatataatc attcacagaa aagataataa
    14041 ctatctgaag caacgaatta atgatgagaa caaaatatca atagtcaccg aagcaatgaa
    14101 tgaagcactt cgtaaacttg agcaacgtgt attaaatacg ctcaatgaat tttctggtta
    14161 tactcatgtt atggttatag gcggtggcgc agaattaata tgcgatgcag taaaaaaaca
    14221 cacacagatt cgtgatgaac gttttttcaa aaccaataac tctcaatatg atttagttaa
    14281 cggtatgtat ctcataggta attaatgatg gacaagcgca gaaccattgc cttcaaacta
    14341 aatccagatg taaatcaaac agataaaatt gtttgtgata cactggacag tatcccgcaa
    14401 ggggaacgaa gccgccttaa ccgggccgca ctgacggcag gtctggcctt atacagacaa
    14461 gatccccgga cccctttcct tttatgtgag ctgctgacga aagaaaccac attttcagat
    14521 atcgtgaata tattgagatc gctatttcca aaagagatgg ccgattttaa ttcttcaata
    14581 gtcactcaat cctcttcaca acaagagcaa aaaagtgatg aagagaccaa aaaaaatgcg
    14641 atgaagctaa taaattaatt caattattat tgagttccct ttatccacta tcaggctgga
    14701 taaagggaac tcaatcaagt tattttctta ccagtcatta cataatcgtt attatgaaat
    14761 aatcgtttgc actgtctctg ttattcaggc aatttcaata aaggcacttg ctcacgctct
    14821 gtcattttct gaaactcttc atgctgcatt tcgatttttc ttctaaaatc ataacttgca
    14881 tacctgttct ttttatgtat atccagatct atttttgtga cgagttcaaa ccacaatgat
    14941 agcactcccc atgcagcgtt atgaaacatt tccgcaacag atttagagtc tgtattaacc
    15001 gcttcagtca tgtagtcgct aatacgttct gcagtgattt tcgttatttc atcgtaattc
    15061 attttatccc ctacaagact atttaatgga atgaaaaata tttggtctga taacaatgct
    15121 cctgaattaa cacctgcagg ctatgttaaa gttgccctca cacacaatct acctgttttc
    15181 tcgtgtgtgt cacaggcgca atacataaat agtctggcca ttaagttcgt aacggatttc
    15241 gtactcgcca gcaaaaatcc gtctgacatc cctggtttca aactgaaaca actgttcatc
    15301 ccatacgtgg atgagttaac agaatgaccg gcgcctgtgt cagggactac accgttttgg
    15361 ctgcagctgg tttactgacc aacgccagaa aatcatataa ctgcgtcaat gtcagcgtat
    15421 ccagcgcgac tgtattattt ttctctgcaa ccagaatatt gcattaggtt attaccaggt
    15481 gttctaatgt acaggtaaag acgttcttta taatctgaaa tctctacttt taatccctca
    15541 gactttgcct gctgaaagcc ctcgtcaagg tgcgctttta ttaggttaat gtctggatgt
    15601 cttgtatcgg gggcaattct gtattggtgt aacgatgggg tggatgttcc ctgcaataag
    15661 acctccagat agagattggc acaaccattc ccattatttc t
//
LOCUS       DAZOZD010000086        15596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOZD010000086 DAZOZD010000000
VERSION     DAZOZD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.086"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..880
                     /gene="yieE"
                     /locus_tag="ACHUPB_002194"
     CDS             <1..880
                     /gene="yieE"
                     /locus_tag="ACHUPB_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0758999.1"
                     /translation="HITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQL
                     GVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYI
                     LPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLED
                     ELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELG
                     NSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSN
                     ALRRFLDYCDPANVPR"
     gene            1085..2554
                     /gene="lysP"
                     /locus_tag="ACHUPB_002195"
     CDS             1085..2554
                     /gene="lysP"
                     /locus_tag="ACHUPB_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0759000.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2846..4837
                     /gene="cirA"
                     /locus_tag="ACHUPB_002196"
     CDS             2846..4837
                     /gene="cirA"
                     /locus_tag="ACHUPB_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR0759001.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4869..5705)
                     /gene="yeiG"
                     /locus_tag="ACHUPB_002197"
     CDS             complement(4869..5705)
                     /gene="yeiG"
                     /locus_tag="ACHUPB_002197"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0759002.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5963..6631
                     /gene="folE"
                     /locus_tag="ACHUPB_002198"
     CDS             5963..6631
                     /gene="folE"
                     /locus_tag="ACHUPB_002198"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0759003.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6648..7805
                     /gene="yeiB"
                     /locus_tag="ACHUPB_002199"
     CDS             6648..7805
                     /gene="yeiB"
                     /locus_tag="ACHUPB_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0759004.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7989..8987
                     /gene="galS"
                     /locus_tag="ACHUPB_002200"
     CDS             7989..8987
                     /gene="galS"
                     /locus_tag="ACHUPB_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0759005.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            9267..10265
                     /gene="mglB"
                     /locus_tag="ACHUPB_002201"
     CDS             9267..10265
                     /gene="mglB"
                     /locus_tag="ACHUPB_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0759006.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10326..11846
                     /gene="mglA"
                     /locus_tag="ACHUPB_002202"
     CDS             10326..11846
                     /gene="mglA"
                     /locus_tag="ACHUPB_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0759007.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11862..12872
                     /gene="mglC"
                     /locus_tag="ACHUPB_002203"
     CDS             11862..12872
                     /gene="mglC"
                     /locus_tag="ACHUPB_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0759008.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12999..14234)
                     /gene="preA"
                     /locus_tag="ACHUPB_002204"
     CDS             complement(12999..14234)
                     /gene="preA"
                     /locus_tag="ACHUPB_002204"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR0759009.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14228..15466)
                     /gene="preT"
                     /locus_tag="ACHUPB_002205"
                     /pseudo
     CDS             complement(14228..15466)
                     /gene="preT"
                     /locus_tag="ACHUPB_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
BASE COUNT         3711 a         3827 c         4108 g         3950 t
ORIGIN      
        1 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
       61 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgaccgacct
      121 ggaagggcag cttggcgtgc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
      181 acacgggcgg ctgctctatc cgcgtgcgtt ggcattgctt gaacaggcgg ttgaaatcga
      241 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
      301 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga
      361 acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat
      421 tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga
      481 cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg tcaccttaga
      541 acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggagattgt
      601 cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
      661 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt
      721 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tcccgcgcct
      781 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
      841 ctttctggac tattgcgatc ccgcgaatgt accgcgttaa ggtgctgtac aagaacatgc
      901 tggtgctgtg tcgatttcgt gacgcagcgc cctcagcatg cattcgccag aaaagagatt
      961 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     1021 ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg gatagcattt
     1081 ttgtatgggt tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc
     1141 gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc
     1201 tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg
     1261 cctgatggtt tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc
     1321 cggttcgttt gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg
     1381 ctggaactac tggtacaact gggcggtgac tattgccgtt gacttggttg cagcacagct
     1441 ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg
     1501 cgttatcttc ctgctgaact acatctcagt tcgtggcttt ggtgaagcgg aatactggtt
     1561 ttcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat
     1621 cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc
     1681 gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca
     1741 gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc
     1801 acgcgcggta cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat
     1861 tatcagcctg attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat
     1921 cagcgtcagt ccgttcacct tggtgttcca gcacgcgggt ctgctctctg cggcggcggt
     1981 gatgaacgca gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc
     2041 tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt
     2101 gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg
     2161 cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg
     2221 gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg
     2281 ttacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact
     2341 ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc
     2401 gttcctgaaa gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct
     2461 gttcctgatt atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag
     2521 cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct
     2581 ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt
     2641 aacatttgga ttgataattg ttatcgtttg cattatcgtt acgctacaat caaaaaaggc
     2701 tgacaaatca gaggctggtc cggctttctg ggatgatcac ctgcatacaa ataagtccac
     2761 cgcgatgctg ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt
     2821 cgtagttacc tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct
     2881 gtgtttgtcc gctatttctt gtgtatggcc tgtgttagcg gtcgatgacg atggcgaaac
     2941 gatggttgtc actgcatctt ccgttgaaca aaaccttaaa gatgcacccg ccagtatcag
     3001 cgtcattacc caggaagatc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa
     3061 agaagtgcct ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg
     3121 tggtctggac agcagctata ccctgattct cgtcgacggt aaacgcgtga actcccgcaa
     3181 tgccgtcttc cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg
     3241 tattgaagtg gtccgtggcc cgatgtcgtc gctgtacggt tccgatgcgc tcggcggtgt
     3301 agtgaatatc atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac
     3361 caccattcag gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg
     3421 accattaatt gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa
     3481 ggatgacccg caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt
     3541 ctccagccgc gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac
     3601 tgccggatac ggtttcgacc gtcaggatcg tgattccgac tcgctggaca aaaaccgcct
     3661 ggaacgccag aactactccg tcagccataa tgggcgttgg gattacggca ccagcgaact
     3721 gaaatactac ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc
     3781 cgaaagcaat acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct
     3841 cacggttggc ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg
     3901 aaccagctcc aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat
     3961 cttcgagccg ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga
     4021 acactggagt ccgcgtgcct acctggttta taacgccacc gacaccgtaa cggtgaaagg
     4081 gggctgggcg acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag
     4141 caattcctgc cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag
     4201 cgaaagttgg gagctggggc tttactacat gggtgaagaa ggctggctgg aaggggttga
     4261 atccagcgtt accgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc
     4321 tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg
     4381 acggcgcata ccggtattta gctactacaa cgttaacaaa gctcgtattc agggcgtgga
     4441 aaccgaactg aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa
     4501 cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac
     4561 tgctaacggt acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc
     4621 tggtcactat accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta
     4681 taccatctgg aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg
     4741 cgtgctgaac cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg
     4801 acgtcgttac tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg
     4861 gcggactttc acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag
     4921 gcgatgaagt agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct
     4981 ttctggcgcg cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca
     5041 ttatcgccct gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc
     5101 gcgcaactat cccattccag ccatgcattt ttgtcctcac ctaaataggt gctaaacgct
     5161 ttgattcccc acgggacgct gcacggattc acaattggcg caaaggccga aacgctggtg
     5221 tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg
     5281 ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg
     5341 cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa
     5401 aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg
     5461 ctggtgtctg gcatcaccag tacaatcccc agttctgccg ccacgcgctg ggcacccgcc
     5521 ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc
     5581 ggggtgtgat cacgaggggg tgggagaaag atactgaacg tcatcgggca gtttaaggta
     5641 ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt
     5701 tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg
     5761 tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa
     5821 agtcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg
     5881 cacaataatc aggctgtaaa tcagcttaat aactttgccc ccacgcaggg cggaggcgtc
     5941 acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga
     6001 agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa
     6061 cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct
     6121 cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga
     6181 tgaaattttc tccggtctgg attacgccaa cttcccgaaa atcaccctca ttgaaaacaa
     6241 aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca
     6301 ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat
     6361 cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga
     6421 acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc
     6481 tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt ggcatccgcg atgcaaccag
     6541 tgccacaaca acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga
     6601 gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc aggaaccatg gagcgcaacg
     6661 taacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg
     6721 cctttgggct accaaaggcg gcttatctca atcccgcctg gtacggcgct attacgccgc
     6781 aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc ctcacgcttt
     6841 ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc ccgtggcaga cgctggatcc
     6901 agtcgcggtt aacgctgtta gtcttgctgg gttttattca cggtttattg ttctgggacg
     6961 gcgatattct gctggcttac gggctggtgg gcttaatctg ctggcggctg gtgcgcgatg
     7021 cgccatcggt aaaaagcctg tttaatacag gcgtcatgct ttatctggtg gggcttggcg
     7081 ttttgctgtt attggggctg atttccgaca gccagaccag ccgcacctgg acgccggatg
     7141 catcggctat tttgtatgaa aaatactgga agcttcacgg cggcgttgaa gcgatcagta
     7201 atcgtgccga tggtgttggc aacagtttac tggcactggg cgcacagtat ggctggcaac
     7261 tggcagggat gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt
     7321 tcagcttacg tcactatcgt cgtactggtt ttgtgctggt ggcgattggg gtgaccatta
     7381 accttcctgc catcgccctg caatggcagc tggactgggc gtatcgctgg tgtgccttct
     7441 tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt
     7501 tttatggctt ctggccgcaa ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg
     7561 gacggatggc gctgaccaac tatctattgc aaacgctgat ttgtaccatg cttttttacc
     7621 acctcggttt gtttatgcat tttgaccgcc tggagctgct ggcgtttgtt attccggtat
     7681 ggctggcgaa tatcctcttc tctgttatct ggctgcgtta cttccgccag gggccggtgg
     7741 aatggctctg gcgtcagtta actttgcgtg ctgccggaac ggcaatatct aaaacatcaa
     7801 gataacgatc tggatcacat tcgttaacaa aacggctgta accgtttcca ttgctgtgac
     7861 tcgattcacg aagtcctgta ttcagtgctg acaaaatagc cgccagcaag cagtcattta
     7921 ctgcaatctc ataacaggta gtgaatatga tcaccattcg tgatgtagcg cgttaggctg
     7981 gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag cacgctggtc agtgccgaca
     8041 cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta tcggccaaac gccaatgccc
     8101 aggcgctggc aactcaggtt agcgacacca ttggcgtggt ggtgatggac gtttctgatg
     8161 cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc tcagcagcat cagaaatacg
     8221 tgctaatcgg caatagctat catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa
     8281 ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc attgagtgac gatgaactgg
     8341 cgcaatttat ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg
     8401 cccatcgttg cgtttgcctg gataatctca gcggtgcccg aatggcgacg cgcatgttgc
     8461 tgaataacgg tcatcaacgt attggttatc tttcttccag tcacggcatt gaagatgacg
     8521 ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca ggatattatt ccgccggaaa
     8581 gctggattgg cactggtacg ccggacatgc cgggcggtga ggcggcgatg gttgaactgc
     8641 tggggcgcaa tctacaactt accgctatat ttgcttataa cgacaatatg gccgctggcg
     8701 cactgacagc attaaaagat aatggcattg cgattccgtt acatctctca atcatcggtt
     8761 tcgatgatat tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg cgttatccca
     8821 ttgcttcaat ggctaaatta gccaccgaac tggccttgca gggggcagca ggcaatattg
     8881 atcctcgtgc cagccactgt tttatgccga cgttagtgcg tcgccattct gtcgcaacgc
     8941 gccagaatgc ggcggcgatc actaactcaa caaatcaggc gatgtaaccg ctttcaatct
     9001 gtgagtgatt tcacagtatc ttaacaatgt gatagctatg attgcaccgt tttaacgttg
     9061 taacccgtat gtaacagtga ataatcactt tcaccgaggt aatagcgtca taacaacaat
     9121 taaagccgtt ttctggagcg ttaccgggca tggaagaacg aatttaaaaa agtgagcttc
     9181 ggcgttcagt aacacttcat taactctact gccccgccga gcatttatct caagcactac
     9241 cctgcataag aaaaaccgga gataccatga ataagaaggt gttaaccctg tctgctgtga
     9301 tggccagcat gttattcggt gccgctgcac acgctgctga tactcgcatt ggtgtaacaa
     9361 tctataagta cgacgataac tttatgtctg tagtgcgcaa ggctattgag caagatgcga
     9421 aagccgcgcc agatgttcag ctgctgatga atgattctca gaatgaccag tccaagcaga
     9481 acgatcagat cgacgtattg ctggcgaaag gggtgaaggc actggcaatc aacctggttg
     9541 acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt
     9601 tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc tactacgttg
     9661 gcactgactc caaagagtcc ggcattattc agggcgattt gattgctaaa cactgggcgg
     9721 cgaatcaggg ttgggatctg aacaaagacg gtcagattca gttcgtactg ctgaaaggtg
     9781 aaccgggcca tccggatgca gaagcacgta ccacttacgt gattaaagaa ttgaacgata
     9841 aaggcatcaa aactgaacag ttacagttag ataccgcaat gtgggacacc gctcaggcga
     9901 aagataagat ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg
     9961 ccaacaacga tgcgatggca atgggcgcgg tagaagcatt gaaagcacac aacaagtcca
    10021 gcattccggt gtttggcgtc gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg
    10081 cactggcggg cacagtactg aacgatgcta acaaccaggc gaaagcgacc tttgatctgg
    10141 cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac caactggaaa atcgacaaca
    10201 aagtggtccg cgtaccttat gttggcgtag ataaagacaa cctggctgaa ttcagcaaga
    10261 aataagactg attgggcgca gtcgtttact gcgcccttat tgtatggcca acaaggtaaa
    10321 attatatggt cagctcaacg actccgtctt ccggggaata cttgttggaa atgagcggta
    10381 tcaacaagtc ctttcctggt gttaaggcac ttgataacgt taatttaaaa gtccggccac
    10441 attctatcca tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc
    10501 tgtttggtat ttatcaaaaa gactccggca ccattttatt ccagggtaaa gagatcgatt
    10561 tccattccgc caaagaggca ctggaaaatg gtatttcgat ggtacaccag gagttaaacc
    10621 tggtattaca acgttcggtg atggataaca tgtggctggg gcgatatccc accaaaggca
    10681 tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc aatttttgat gaactggata
    10741 ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg
    10801 aaatcgccaa agcgttttcc tataacgcga aaattgtgat tatggatgaa ccgacttctt
    10861 cgttaaccga aaaagaggtc aatcatctgt tcactattat tcgtaaatta aaagagcgcg
    10921 gctgcggtat tgtttatatc tcgcataaaa tggaagaaat cttccagtta tgtgatgaag
    10981 ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc gctggcagga ctgacgatgg
    11041 acaagatcat cgccatgatg gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa
    11101 acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga
    11161 ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct cggtattgcc gggctggtgg
    11221 gggcgaaacg taccgatatt gttgagacgt tatttggtat tcgcgagaaa tcggctggca
    11281 ccattacgtt gcacggcaaa cagatcaata accataatgc caacgaagcc ataaaccacg
    11341 gatttgcact ggtaactgag gagcgccgct caacgggaat ttatgcctat ctggatattg
    11401 gttttaactc gttaatttcc aatattcgca actacaaaaa taaagttggt ttactggata
    11461 actcgcggat gaaaagcgat acccagtggg tgattgattc gatgcgggta aaaacgccgg
    11521 gtcatcggac gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg attattggtc
    11581 gctggctact aacgcaacca gaaatattaa tgctcgatga accgacgcgc ggtattgatg
    11641 tcggggcgaa gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga
    11701 ttattattat ctcctctgaa atgcctgagt tgttagggat aacagaccgt attctggtca
    11761 tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc
    11821 tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc catgagtgcg ttaaataaga
    11881 aaagttttct tacttacctg aaagagggcg gtatttacgt cgttctttta gttttgctgg
    11941 cgattattat tttccaggac ccaacatttt taagtctgtt gaacttaagt aatattctca
    12001 cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt gtcacccagg
    12061 ggaccgatct ttctgctggt cgtcaggtag ggctggcggc agtggtggct gcgacattat
    12121 tgcagtccat ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc
    12181 tggttattct gattgtctgt gccattggtg cggtgatcgg tttgatcaac ggtctgatta
    12241 tcgcttatct caacgtgacg ccgttcatta ccacgctcgg cacgatgatc atcgtctatg
    12301 gcatcaactc gctctattac gactttgtcg gggcgtcgcc aatttctggt tttgacagtg
    12361 gcttctctac ctttgctcag ggctttgtcg cgctggggag tttccgtctc tcttacatca
    12421 ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg
    12481 gtaagaacat ttttgccatt ggcggtaacc cggaagcggc aaaagtatct ggtgtcaacg
    12541 tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt gttctatgcc tttggcggga
    12601 tgttagaagc cggacgtatc ggctctgcca ccaacaacct cggctttatg tatgagctgg
    12661 atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg gggacggtga
    12721 ttggcgtggt gaccggggtg attattttta ccgtcatcaa ctatggcctg acgtatatcg
    12781 gcgtaaaccc atactggcag tacatcatca aaggggcgat tattatcttc gccgtagcgc
    12841 tggattcact gaaatacgcg cgtaagaaat gatgtgaatg tggtccgcat tcggtcataa
    12901 acgcacataa catcatgatc tgtgtggttt tgtaggccgg ataaggcgtt cacgccgcat
    12961 ccggcaacag gaaccaaatg ccggatgtga cgcgggttct acaacgttac cgggtgttct
    13021 ttctcgcctt tcttaaactt cacttccccg agctcaatac aacccaccgg gcagacgtga
    13081 ccacaaagca gacaacccac acatttctcg gtattacaat gcggtgtgcg ggttttctcg
    13141 ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg tccacagcca
    13201 acacatttgt caagattgat acggggatag acaatataac tgcggtctaa atcttccgcc
    13261 gggacaatat tgttattcgc cagacctacc atctcctgca acgaatcaaa tccctgatcg
    13321 gcgagataat gcgataaccc gctcgccata tcttctacga tgcgatagcc gtactgcata
    13381 atgccggtgg tgacctgtaa cgttgctgcg ccgagcaata aaaactccgc cgcatcctcc
    13441 caggtttcaa tgccgccgat accgctgatt gggaaatcgc gcagttctgg atgggtgcgc
    13501 atttgctgaa tgaagcgcag ggcgatcggt tttaccgctt taccggaata tccggaaata
    13561 cttgattttc cgttaacgat cggcataccg attttctgat taagatcgat attggtgatg
    13621 gatttaacgg tgttaatcgc cgcaatgcca tctgcgccgc cgcgcttcgc cgccagcgcc
    13681 acttcgcaca tatcaccgat attcggcgtc atcttcgcca gcattggcag cgtggaaccc
    13741 cgtttcactg cccgacaata tttttctacc agctccgggc tttgcccgac atcgctaccc
    13801 atcgcatgag aagtcatttg cggacaggag aagttacact cgatcatatc cgcgccagct
    13861 tcttgcacca ggcgcgccag ctcctcccat tgctgctcat tttcccccat gatcgaagcg
    13921 atcaatactt tgtccgggta atcttctttc agccgacgca gggcggccag attctcctcc
    13981 agcggatgct cggcaatctg ctccatattt ttgaagccga taaaaccggt atcttctttc
    14041 accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat cgttttaaac
    14101 accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca gttgcctacc
    14161 ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaagt aatcgaaaga
    14221 tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg gcggcttctt
    14281 tcccggtttt cacggcatag accacggttt tgtctccctc aacaatatcg ccagcagcaa
    14341 agacttgcgg gtcgcgggtc tggtaatttt gtgttttgat ggtgttacgc tgcggctcta
    14401 actccgcaaa ggcatctagt ctggcatgct gaccgacggc gagaataatt ttatctgccg
    14461 ccatcgtcag ttcgcccgat aaccgtacat gcttaaacgt gactttattg ccttccacgg
    14521 ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg gtaaactctt
    14581 tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc gcctgacagc
    14641 cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg ataattaatg
    14701 cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag aagtcgacgg
    14761 caatctcaac gtcactatgc tcaaacagcg gtagcccgga accgcttgat aaccccacgg
    14821 tgaccagtac cgcgcggttt tctgctttca gctgctcaag ggtgagtgtg ttaccgactt
    14881 cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct gcatccagta
    14941 ctgattgcgg taaccggaat tgcggaatgc cgttacgcaa tcagccaccg ggttgtgctt
    15001 ctttctcata gatcgtgacg tcataaccct ggtttgtcag tgtcacactg gcctgcaatc
    15061 cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg ggctgataaa
    15121 tttccatccc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg ccgatatcaa
    15181 tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc gtcgggcaca
    15241 ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca gcgcctttaa
    15301 aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc gggcaagcct
    15361 gactacaggg agcgtcatga cataataaac agcgagaggc ttctttaatc gccagtaaag
    15421 gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga tcttccttaa
    15481 tgctatgagt aattaaggcg aacgctaaat aaatattgcg tgctgatatt aaggctaagc
    15541 gtgtggggaa atttaaactg tgaaaggatt cacaaatagc tgcggttcag aatgtt
//
LOCUS       DAZOZD010000087        15594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOZD010000087 DAZOZD010000000
VERSION     DAZOZD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.087"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..141
                     /locus_tag="ACHUPB_002206"
     CDS             <1..141
                     /locus_tag="ACHUPB_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR0759010.1"
                     /translation="IIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDKLAPVISL
                     MK"
     gene            complement(122..463)
                     /gene="trbA"
                     /locus_tag="ACHUPB_002207"
     CDS             complement(122..463)
                     /gene="trbA"
                     /locus_tag="ACHUPB_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR0759011.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(477..1220)
                     /gene="traF"
                     /locus_tag="ACHUPB_002208"
     CDS             complement(477..1220)
                     /gene="traF"
                     /locus_tag="ACHUPB_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR0759012.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(1213..1470)
                     /locus_tag="ACHUPB_002209"
     CDS             complement(1213..1470)
                     /locus_tag="ACHUPB_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR0759013.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(1497..3401)
                     /gene="traN"
                     /locus_tag="ACHUPB_002210"
     CDS             complement(1497..3401)
                     /gene="traN"
                     /locus_tag="ACHUPB_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR0759014.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(3637..4017)
                     /locus_tag="ACHUPB_002211"
     CDS             complement(3637..4017)
                     /locus_tag="ACHUPB_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759015.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(4014..4652)
                     /gene="trbC"
                     /locus_tag="ACHUPB_002212"
     CDS             complement(4014..4652)
                     /gene="trbC"
                     /locus_tag="ACHUPB_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR0759016.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(4661..4966)
                     /locus_tag="ACHUPB_002213"
     CDS             complement(4661..4966)
                     /locus_tag="ACHUPB_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759017.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(4996..5988)
                     /gene="traU"
                     /locus_tag="ACHUPB_002214"
     CDS             complement(4996..5988)
                     /gene="traU"
                     /locus_tag="ACHUPB_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR0759018.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(5985..6617)
                     /gene="traW"
                     /locus_tag="ACHUPB_002215"
     CDS             complement(5985..6617)
                     /gene="traW"
                     /locus_tag="ACHUPB_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR0759019.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(6614..7000)
                     /gene="trbI"
                     /locus_tag="ACHUPB_002216"
     CDS             complement(6614..7000)
                     /gene="trbI"
                     /locus_tag="ACHUPB_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0759020.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(6997..9627)
                     /gene="traC"
                     /locus_tag="ACHUPB_002217"
     CDS             complement(6997..9627)
                     /gene="traC"
                     /locus_tag="ACHUPB_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR0759021.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(9753..10100)
                     /locus_tag="ACHUPB_002218"
     CDS             complement(9753..10100)
                     /locus_tag="ACHUPB_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759022.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(10128..10346)
                     /locus_tag="ACHUPB_002219"
     CDS             complement(10128..10346)
                     /locus_tag="ACHUPB_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759023.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(10445..10918)
                     /locus_tag="ACHUPB_002220"
     CDS             complement(10445..10918)
                     /locus_tag="ACHUPB_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759024.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(10911..11324)
                     /locus_tag="ACHUPB_002221"
     CDS             complement(10911..11324)
                     /locus_tag="ACHUPB_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759025.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(11317..11538)
                     /gene="traR"
                     /locus_tag="ACHUPB_002222"
     CDS             complement(11317..11538)
                     /gene="traR"
                     /locus_tag="ACHUPB_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR0759026.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(11673..12188)
                     /gene="traV"
                     /locus_tag="ACHUPB_002223"
     CDS             complement(11673..12188)
                     /gene="traV"
                     /locus_tag="ACHUPB_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR0759027.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(12185..12505)
                     /locus_tag="ACHUPB_002224"
     CDS             complement(12185..12505)
                     /locus_tag="ACHUPB_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR0759028.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(12492..13082)
                     /gene="traP"
                     /locus_tag="ACHUPB_002225"
     CDS             complement(12492..13082)
                     /gene="traP"
                     /locus_tag="ACHUPB_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR0759029.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(13072..14499)
                     /gene="traB"
                     /locus_tag="ACHUPB_002226"
     CDS             complement(13072..14499)
                     /gene="traB"
                     /locus_tag="ACHUPB_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR0759030.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(14499..15227)
                     /gene="traK"
                     /locus_tag="ACHUPB_002227"
     CDS             complement(14499..15227)
                     /gene="traK"
                     /locus_tag="ACHUPB_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR0759031.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(15214..>15594)
                     /gene="traE"
                     /locus_tag="ACHUPB_002228"
     CDS             complement(15214..>15594)
                     /gene="traE"
                     /locus_tag="ACHUPB_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR0759032.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         3703 a         4314 c         3668 g         3909 t
ORIGIN      
        1 atcatctata tttatttttg tgagttattc agaagtcact ggatagcagt ctggtttatc
       61 ctctggtctg tggtaatcaa cctcactatt ggatttggca tttatgacaa attagcacca
      121 gtcatttcac taatgaaata ataaaaaacc acatcactcc agaccatatg gctgacaaca
      181 taatacaaac aaacatcctt ctgaaactgt caaacttcgt gataaccggt atggtactga
      241 ctgccattga aacaaccggt acggcacact gattcataac cgcaccacaa agaaacaaga
      301 aattatctgg cagcatacat acacttatga catttactac aaagaagaca aacgagctca
      361 gcagataaac tcctttatta tttggtcctg taaagaaata accaacaacc cccacgataa
      421 acaggctggc acctgtaaaa aaacacaaat cccctgtatg catatataac ctttggttaa
      481 aaattcggtt taaaatcttc agaaacgttc aggaactgtt ttgccaggtc gtcctgtgaa
      541 ataaagccgt atgataacgg gcgaacactg ccctgtttcg ggtcaaccag catcatggcc
      601 gggaaatatt tcacaccgag gcgctgcgcc tgcccctggt cagtccggga atccggcaac
      661 agcggattaa tcacgccatc cacggaaacg ggaataacac tcagaccata cgtatcccgg
      721 aagccattaa tgacctgcgc cagttgcccg tcgatggggt cctgcccccg gtaaaaaaac
      781 atgatgccgt agtgttcagc cagtttcgca atggcctgtc gctgctgcgc ctggtctgcc
      841 gccagctggt tccgtaccgt gccgttgtaa tggctgtact gcaggttata gtccagttcg
      901 ggatgtgcca gcatggcctt tttagcactc atggtgaaaa gcccggcctg ctgagtccag
      961 taattctgca gccggaaata tttaacaaaa ttatccacgc cgggatacag aatggcttcg
     1021 tacagcgccc gctttgttgc cgtctgcagc gcggccagtt tctgcataat atccggctcc
     1081 tgacgggggg ctgcaggtac aggtttattt tctttttcct tcggatttat tttctcgtta
     1141 taccactgcc agcctgcatc tttccctgac gccggaaaaa taaagcagca gagtaacagt
     1201 ggcagtaatg ctttattcat cttcattcct ttcatcattt tttctttcat catctttctg
     1261 caggccagtc agggcagaaa aacaaaaata cagaataaac aggagcacac cggagtaaag
     1321 gataacgaaa gcagcagcaa gtatcccgtc tgccgtagtt tcttttaccg tatcccacga
     1381 ccagataatt accggagaaa tgacaatcgc tgtaatcgaa agccggataa gaaaatcaac
     1441 aagacgatta gaagtgaata taactttcat catttccccc ggttattttc cataccttac
     1501 tgtcctgcct gtttcagctg gtcagcaatc tgctctttca ctttctgcgt cagaacgcca
     1561 ctgtccggga ttttctggtt attcatcagg tcttcgtaga agttagtgaa atccagtctg
     1621 tcgaactgaa ttttctgcag ctcatcaacc gtaatccccc gacagtcagg atgcttcgca
     1681 ctgccaaacc cgatacgcaa ctgcccgtta cgcccctgct gctggactat ctgcgccagc
     1741 ttcgaatcaa actggcagta gctgcgcttt ttctgcagac agacacccag cactttccgg
     1801 gagcagaact cgcccacact caccgtcagt ttgttgtcct ttgcctttcc cagcgccttt
     1861 tcttcactgt tgcacttcgc cagccccaca tcctgacccc agccgccatc cttacagcag
     1921 ttactgaagc cggctgcggc cttacggcag aatttcgcct cacccgtgaa agccctgaca
     1981 tccacaccat tcagggcggc aacatcctta cctgcggcgg caagcgcagc cagctgtgat
     2041 actgcttcac caaaatcact gttttttccg ctctgtgcct tatcgcattc accgtcaagg
     2101 cagaagacat caccaccaca gaccatcact tttcctgatg ttctggactc gcaggagtac
     2161 gtggcgtact catgcagaca ggttccctca tcggaataaa acgcgcactg acgtgaggcc
     2221 agcgtacagg ccggattatc gacatatttc tgacaggtgc cgttatctgc ggactgagtg
     2281 acatatttat cccggtatgc ccagcatgcc tgtgtgatgt tccagggttt accttccatc
     2341 actccggttt tcgtaccacc cggctctgaa cactcagtgc ctgtcagcac gccatccgct
     2401 ttattaaacg gacaactctc cacccactcc acccggggaa cccattcttt atcacggacc
     2461 ttcatcctga gtttcagcgt aaaggtggaa gcaccactga caagcgattc atagactttc
     2521 ctgtcaccat tcccgtgcgg gaggcaatta ccgttagcag tacagccact accggtcaga
     2581 acctgcccct gtgtcacctg caaacctgtc gccccggaca gggggtaagt atccgactgc
     2641 ccgtttgcca ggccgacatt aaaggttgta ttcatgaagg tataacggga gttcaggaag
     2701 aaaaaagcgg cgtatacaga caatgatgca ctttccactg taccggttac cggagccgtg
     2761 acactgaaca ccagtttcag cccgttcata ctgaaccgga actgtgagtg cggtatcgtc
     2821 acttcacgat attcatccgt gtatttccag tccccggtga tactggcagt ccgtgtgcag
     2881 tactcctcca ccatcgtgtc ccgctcacag gtatagtttg tgaattcact gcggtttatc
     2941 tcctgcgcac tgcactgctg tccggtattt cccacaatgc tgtcagccct gttcaccaca
     3001 tccctgccgg tctgaatgaa cggtgcatcc ggtgacagaa tgtctttcgg cttgttcatg
     3061 aaggactctg tgatggtttt tccggtttca ccggtcgccc attccgtggt gccgtcattt
     3121 ttcaggccgc catccccgcc ggcagtcacg ccaccgtaat attttgtctc gtccggattc
     3181 gcgttatagc cggggatact ctcctgtggc ttaaagccct gaatactgct gcttccctgc
     3241 cctttgatct ggtgagcaaa atcagaaccg gcccggtaat cactgttgct gtcagcaaac
     3301 acagaaaagc tcagaaaaaa aacaattgca caaagattgc acggctttac ggaataatta
     3361 aaattcagag atctgaaaaa ggtatttatc ttttctttca taacctgaac catactggag
     3421 aatgaagtga tgtcatctca acttcagaac tgtgttgatt gtggtaaagc attaagtcga
     3481 acagccacaa cgtgcgggaa ttgcagctca acggaccctt ttggaaaaaa acggcgcagc
     3541 gatcgctttt accagtggtt tgtagggctg tttattacgg ctattattat tgctggcgga
     3601 ttatggtaca tggggcttct tgacgtaccg ggattactac tcagataacg acttcagtaa
     3661 cgatatcagt gttctgaaat cggcacataa cgagtgacga caataccggt tataccagta
     3721 atgaggggtg tcgggctgaa gcctgaaaca gagctgaaac ttactgttaa aagccccctt
     3781 caccgaccat ccccatgcca gacgtggtat tgctgattca gcatcctgcc gcagtcgctg
     3841 ctcaagaagg gcagccattt ttaccggaca ggcaaaggca atataaggag gcagctttgt
     3901 aacagggaaa ccatgtccct gacaggaagc atatgtgcgg aagccgtgaa tgttcatcgc
     3961 ggccaccagg gcacggatct ccggatcaat ctgataatac ccggcaggta aagtcatttt
     4021 tgtttatctc ccgggttatt cagcaacgcc cgagccacct gccggcagtc ccccgtggcg
     4081 gccactttct ccagcgcctg gccgacccgc aggttacctc ggataatgtc gtatccctga
     4141 ctgcagaaca ccaccagtgc cggtacactg cgaatgccgt actgtgaaaa cagcgtcggg
     4201 tcgatctgaa caccatcagt cgcgccgtct ttcaccaggg acagcacggc ttcagcggtg
     4261 gtcttcaggt cattattcac catgccccgc agtgtggcgg gtataccgta gtgccgggtt
     4321 tcacccagca tccgtttcag cccctcttcg ggaatggaaa acgacacaaa atacagggca
     4381 ccctgccggg gtttcccgtc ctgcgaagcc tgctgtttac gaaccagctc atccagaaaa
     4441 tggttatctg aacgctgaag ggggttttcc agcacctgtt tctccgccca ggctttcagc
     4501 tgatggtcag gtttctctcg cagttgtctg cttaaatttt cctgctgctt caggaactgg
     4561 cggttttcag gtgtgttcac attttctgaa gcatgaacag caagatgcat ccctgctgtc
     4621 agagcaaaaa gtgcaaatat tttccggttc atgatatttc tcactttctg ctatttacag
     4681 gcatgaactc atcagaaaaa agataattga gaaaaacagc cataaacgca catgtaaatg
     4741 tcaccttcag atataatctg acaagtgtca cttgttctga tatatctgcc ttcttccatt
     4801 catgaataat ttcagcgaga aaactgtcag gtgcaaaata aaataatata acgggcacca
     4861 ttacaagacc aaaaggaaat aattttaatg ttctgcttat cagcctgagc agcacagcac
     4921 ttattttcct gaggtgctga atatatttac tgtccattgt tgccattttc atttcctctt
     4981 attcattaaa atgatttaca ggaatacaca gttacgttta cgccacatca ggtagccgaa
     5041 gtttttcttt gtgttgggcg gattttttcc cgtctcccag cgcatcacac tgcgcccgaa
     5101 cggatggcac tgcccgctgt ccggatacat attcaccatc tgataacgcc agcgttcttt
     5161 gggcaggatt ggggacggat attcattaca gacggcgtta tttttcccga tggtttccat
     5221 aatcatgccc tgacggtgta gtttgaacgc catgcgttca ctgaccagca gggaggactg
     5281 caacggactg gactcattac tcacccagcc attgaacggg tacatacttc cctgcgaacc
     5341 ggcacaccag aacagaacat cgagaggcat attaaaggcg ctggcaatgg catctgctgc
     5401 acaggccccc tgtgctatcg gattggcaaa gatgacagct tccggattga gaatggtggt
     5461 caggctgctg tccgtccagg tggggtcaat ttcagaaaga taagcgatat ccaggtcacc
     5521 accttccaga cagcccagcg atgtgatgat gttcagccag tacgtcagcg ggtatttgta
     5581 ccagtgaacg tgatagaacg ccccgttcac ctgcttctca tcctttttcg ccgttccctg
     5641 tgccgtttta ccaaaagccg gcaggctgaa gcccaggtta accatgcatc ccggtgaccg
     5701 ggtgacgtcc gtcagcgcca tcggctccca gtaaccaatg gccagcccga tacgcctgaa
     5761 cagcggcggc ggtgccggac aaatctgaat gggcatcgac gggttcgccg tgtcggggac
     5821 cttaccctga ctgactttga cactgcccag cgagagcggg aaaatacagc tccagcagat
     5881 atccgtgatc gggtttacaa aacgcccctc acaggcagaa tccgcagacg ctgcagggac
     5941 atgaccggcg agaaggaata acatcagcag ccacaggctt cgcttcattt tctgccctcc
     6001 tctgccggaa taaattccac cttcaggaag cgatcgccgg gaacggcgct cacccgtgcc
     6061 gggacctgat caatgccgag gcgctggcag agcacaccgt tctggtcaaa gtaaacacgg
     6121 ctgtccagag acttctgcat ctccgggata ctgccctgca cgaggataat tttgctctcc
     6181 agtgtgggcg gcgtctggcg tttcatccag gccacctgcg ccggatcatc gccattgatg
     6241 aaatacagcg tctggttaaa aggcacatac tgcagcggat tcatcacttc accctgacgg
     6301 gcaaacaccc gcccttcgtt atcccggata tccgcagcca gtctgacgga cgggtcaaac
     6361 aaccggctgc cgtatttctc cgtgcgtccg ataccgggca cggcaggggg ccgcagactg
     6421 ttgcggatca cccgctcctt aaaggcatcc attttccggc ccatctcacc ggactgctcc
     6481 agtgccgtca ggcgctgcat gatcaccgtc agcatgtccg gctcctttac cggccacaga
     6541 tcgccccagg tgccaagatc ggcggcggcc acactctgcc cccatatcag cagggcaatc
     6601 agcccccggc atctcatggt tccgctctca ttcgccgggc aatatcctgc tggatttccc
     6661 gggtgatatc cggagccccc tgtaccacgg caggcgacac cagaatgacc gcgtggtgtt
     6721 tctgctgcca cgcctgcagg ctggcttcca gtgccgtgtt aaaacgcgct gaaagggctt
     6781 ttgactgtgc ttctgacagt tgtttctgac tggcgctgtc aaaaaatgca tccacagtct
     6841 gtttcatgtt gaaggcggca gtcaccggtg cgttcagtct gacaatgccg taactgacgg
     6901 ctgcgttcag taacaccatt gccaggcatc ccggcaccgt ccaccaccag tggctgcgtc
     6961 ggggggctgt aacatcagtc gttttttgcg ttgtggtcat gcaacactcc tgtatttctc
     7021 atgttcttcc agccaggctt ccagcgaagc catctccggc cctgacttct tccacgccag
     7081 ctgccacacc gcctcatgaa tactcaggcc ctccctgcgt ttctgctgca caaactcaaa
     7141 atccgggccg tcagagctgt acatggcgcg gcttaacggg tccacaaaca gacggtgcca
     7201 ggaggaatgg ttttccacct gcagcaggaa ggaactgaac cactggtctt tggccgcacc
     7261 aaacttgcca atcatgtcgc gctgcagtgg ctggaactga tccgggaata actggttata
     7321 tttcgcgaac tccttcgcac tctgtttgag gataattttg taggaggagt taccccatgc
     7381 cgcacgggcc gcactggagg ccttgtcaga gtcaaagtcg acgatgttct gtgtgatggt
     7441 gatataggca ccggtatggc ggcgacaggt gcggtaacct ttttgaataa actcaccgac
     7501 cttgcgattt ttgaagtcca gcagacgcca gccttcatca atgacgttca gtttcttgag
     7561 agtacgcggc gtgcggtaca tcctgttctc gatgtagata atcagggaga acatcaccgc
     7621 aaccagcagt gacggacggt cttccagtcc gcccagctcc agcaccacca ttttcgcatc
     7681 atcccgcagg gacggctcat cggagttaaa atactggccg taagtgccgt tggcagtgta
     7741 ctggtcaagc agcacaatca tttcgtccag acggctgcgg atggtcggtg acccggcata
     7801 ctgctcgctg tcactggcgt ttttcaggaa atccaccacg tcatcaatac gtgccctgtt
     7861 ctctttggcc agccaggagg ccctgaccgc ctgcagcagc agaccttcat gcacttcatc
     7921 caggttaccg ttggggctgg ccatcactga caactggtca cggacacgct ccgctgactg
     7981 gtcaatatcg gtgatgttcg caaacgggtt aaaacgcagg gtttcaccgt ccagatatac
     8041 gccccccatg ttctcacaca gggacttgta gccatccccc atgtcgaaca ccacggcaaa
     8101 gcccccggag tccagcacgc tgcggataag tggctgtatc agtccggttt taccggcccc
     8161 ggaggtgcca cagaccgcca tgttgtagtt ggtgttattc atccccttaa agaagatatc
     8221 gataaacgcc agctggttac ggtaggtggg cgccagcaga cctgccggtg tcagggggtt
     8281 atccgccact aacggcatca ggttggccac attaaagctc tctgcgcgct gaaccacacc
     8341 ggcctctttc agctgtttaa acagcccttt cccggccata aagggcaggc aggtcaggaa
     8401 attgcgcatg tggttaaagc gcggtgaaat cagctcaaaa ccgtttttac ggaagctgtt
     8461 caggatgtcc tgctccactt ccagtgccgt ttcattattg tccttgcaga aggctgtgat
     8521 gttgaggaag taggacacga cagaagactg gccggagccc agccgctggc gcagttcccc
     8581 ccactccttc gcctctttct ccacggacgg aaaccatttg gcatatgagg tcttcgactt
     8641 cttctccagg tccatgtact tcaggttggc ttcgctgtgg gttttcacct ggtcttccac
     8701 caccagagtc agcgtcagga tgaacgggca ggaaatggac atttccgggt tcagcagatt
     8761 gctgtagttg tcggccatgt tccacaggaa ggcgatttcc gggttacggg ccagatggaa
     8821 attcaggatg cgggccgtgc tgttcctgcc gttctcacgc aggcccagcg tcaggtaatc
     8881 agcccggaca ttcaggtcaa aactgtcctc cacacactga taattcagat cagaatacgg
     8941 gtccagctga cgtcttttcg ggtacagaga gtccggatta tggttaatca tctccccgac
     9001 aatctcgatg aaagcctgtg catccactgt ctgtgtggtg atttttgcgc cgtgaaaaga
     9061 ggcacggatg attttcacca gattttccat ttccagaatg tcagcccggc tttttttctt
     9121 cgagggagaa cagtacgaga taaacacccg gtaatgacgc agggtcaggg gcagattcat
     9181 cccctccggc agcgggaact gtgtcgctgc cgctttcata taataggcac gggtaatggc
     9241 gttaaaccgt tcagcctgtt cacctgacca ggagaactca cgcagcccgt attcaatcct
     9301 gtcaccaacc agctgactgg acatcagatg gatacacaac ggaataccgc gcggcagttt
     9361 ggtgcgcagc atgtgatcga gtgcctccac aatggactta ttcgccccat tgatgggaat
     9421 ggcttccagc ataaagccca tcgtggtgtc attcatgaac agaccggatt cctggttgta
     9481 atcacggtac ggcagcagac ggctgaactg cgggaagctc atttcgccca gaacttcatt
     9541 ggcctttgcg gattcgtcag gcagtttcag tgctgtgacg agggagttaa ccgcctgagt
     9601 gacggcctca agtgggttat tactcatatg agtattcctt agtgcgttta aaaaaggaaa
     9661 atatcagtct gtggccgtgg atatatcacg cagtgccatt cctgaaacca cagcacaaat
     9721 aagagatgga ggcttttaac aggaaaccag gattaacgtt cctttactga atgagcatca
     9781 tttaatgcct gaatttgttt ttcgcgaatc ttattgcgct cattttctct tacccgtttc
     9841 acctctgcca tttcgtttct ttttctgcat tctgatgcga ttttatttgc cttaccaagt
     9901 gttaaaggtt cagactgata attaagtgtc tcctgaaatg cttctgctgc acagggagtt
     9961 tcctgtaaaa tctctgtcag ttgttctggt gttgcaggtt gttgcgcaag gtaaacgatg
    10021 taaaaaggaa tacatacaat catagccata acgccaacaa atataaaagc cagaacagat
    10081 tttaaatata tcgacttcat ttcattttcc ttcgaagcag taagcactca ttgcccacca
    10141 ccatttagtg tttccatcac gctgttgaaa ggttcatttg ttttaacttc aatacactcc
    10201 cctgtatttc caaagcccct gccgccatgt gcttcgattg tccgaagaac ctgcacgcat
    10261 acgtccgtat ttggcatggg tgtaatattc cagcttccgc caccgttaat attttttatc
    10321 agcacaagaa aaataaaggt atgcattctt tattatctcc gcttgtatat taatcactga
    10381 atatcccttc agttaactta tgaggtcata aaacactgca gattttcatt cagatttact
    10441 ctcttcagca aactttactc tcttcagcaa acttccggat ggtatcctgt tcacgctgac
    10501 gggtggcaga ctcctgcatt gcatccactt tctcctgcca gagacgccgg atatcctttt
    10561 catcccgacc agtcagtttt ttaccggaca gcagacggtt aagcagttcc tgtctcgcat
    10621 ccggtacgtc ttcagtcaga cgcataagct gaagcaggct ctgacggctg agaaccatga
    10681 gtgattccgg caggagggtg aagaagaaaa taaaaaagca taccgcacca cagcaggcaa
    10741 ggaccgcaaa caggattttc agatatatac catcaacgcc gaacgcagca ataatacagg
    10801 gaatccatgc aatacacgca caatacgcca ggatgttatc tattcggtta tgtctgcgat
    10861 atatctgttg atgttcatcc agaatagaat ctattttttc agcttctgat ttatgcatag
    10921 tgtttcctct gcgtttcctg ccagagacgc cggatatcct gttcatcccg accagtcagt
    10981 tttttaccgg acaacaaacg gttaagcagt tcctgtctca catctggtac gtcttcagtc
    11041 agacgcataa gccggagtaa ttcctgacga ctgagtacca tgcgtgattc ctccggtacg
    11101 ctgtaataat taaaaatacg actgaatacc ccacaacagc aggcaacgga caggcacagg
    11161 gatttcagat acaggccaga aacacagaat gtggcaacac cacaaagaat agctataact
    11221 aaaaatccct tacccagtgc catacacaaa gaaccctgtt ttctggatat ccgctcatgc
    11281 tcatccagaa taaaatctat tttttcattt tctgatttat gcatagtgtt tcctctgcct
    11341 ttcctgatac gcctgacatt caacgcacaa cgtcacaccg ggaaatattt tccgccgggc
    11401 ttccggaata ggatttccgc atgcttcaca gagataaaca ggaataccat gaacatttat
    11461 ttcctggcgt atccggttta ttcctgtcat ggtcagttgt tctgtcactg aatatgcttc
    11521 atcggcttca tcactcacgg aatattcctc ccgacatgtt ctgacacgct accgcccatc
    11581 ccttttcacg cacagggcac tcgttttaca ggcggatttt gaatacagaa agcccgccgg
    11641 cattgccggc tgatatacaa cttcagggcc agttaattaa tacgtggttt tccccacgca
    11701 gacggtttaa taacaaaaaa cacgctggat ggctgatgat aaacatcctg gttatcaata
    11761 taaggcgcta tccataatgc tgccgtctgc tcacccgttc ttagcggacg aggaggtgtt
    11821 accggcgtaa ctgaactgac cggaacaacc gttttcactt cccgggcagc ggtaaacagc
    11881 gggcgtggtg ccagcagttt ctgctccgga tgtgctgtca ccgcaggtct gctgccggac
    11941 ggagtggtcg cggtgacggt ctgcaccggc attgtccgga agttcccttc agccaggcgc
    12001 ggcagtgatg ccgcaaccgg ctttgcttct gaggactgct ccagtttttt ggccttctca
    12061 ttggcctgct ccatcgtcat acaggtatcg gatgtggtgg cgttacactc aaattccgta
    12121 tttgttcctg cgcagccgga aagtaataag gttcccagca gaggaataaa taaagaaatc
    12181 tgtttcattg cccttctcct tttttattac tccccacaac agcagaaatt aactctgctg
    12241 tcagatttgt tttccctgca cagggatgtc cggagaccac tgtatttccc ggagaagaaa
    12301 ataagctcat cgacattccc ttcttcatca caccgacagc agccatatta aatttgtgcg
    12361 gcaatgattc agaactgaac accttcggag caggaagaac tatttttgtg gtgcagttac
    12421 tgagaacagc atgcataaaa tcatctgata cgtttttatc cggcacaacg cgggctgtaa
    12481 taacattaat attacgcatg ttcattttta tctccgttgc ttctgtttgt tcggtcatca
    12541 gggaaataaa catggcattt cctgattttt cccggcaggg aagcgtcttc cgtataaacg
    12601 tccttcagat attcatctgc ggttaccacc cggatacatt tcatttttcc gggcagcacc
    12661 ggatatttcc cggtgatcgc tgccagcgag acatacatca gtgccagtgt tgcccataat
    12721 gcggtgacca gttgcatcag tgaccggtca gtctccctga cataatctgc agcatcagcc
    12781 gcgcggtaat ggcagatatc ctgtatttct gatctgcctg aagaatcaga ggaagaaaac
    12841 cagcattcac tgaccgggaa ctgtccggca ggtgctgttt gtcttacgaa gttaatctcc
    12901 ttaaccagta atttccccgg cgtggtgttg tccttccata aaacaaacag agccatcagg
    12961 gccattgtgg ccagtggcag aatgacggtg tacttcagac accagaacag tcctcgcaga
    13021 acccgggcga caacataccg ggcggcatgg cacgcctgac gggacgacat attatttgcc
    13081 atcgttgcct cctccctggc tgccggacat aaactgctgc ggattcaggt tgcccagctg
    13141 cttcagcatc tggtcagtat taaagccgtt aagatgactt tcagctgatg gcggaacagg
    13201 aaccggactt tccggattat cttcttccgc tctgttctgc gtgcgctcca gcgccatctc
    13261 ttccacggtt ttcagctgga agccgtcctg gaacaccacg gtcacttcgt tgcccgcacc
    13321 aatcggtatc accgggtgat actgttcggc acgtttgatg tagtagtcac tgagcgtctg
    13381 tgcggctttc gatgtgccgc caccgatacc cattttcagg acatcaccag ccccgtaagc
    13441 ggctgtggca cccagcccga cagccggctg ggaggcgcgc tccattccct gaccgatacc
    13501 gtcaacaaat cccgcgcccc atgcccagcc gaggattttg ccgttacgca tcaccacttc
    13561 gcccttgata ccgtttttac cccggaagct gacatgcccc ttaatcggca tatcaatagt
    13621 tttgccgtcc ttcaggcaac tgatattgcg ggtgcgtaca atggcacgct cactggacac
    13681 atccccccag gcttccagcc ccacaaaaca tcccgttgcg tcatacgtct tgctgttcgg
    13741 catttccacc aggccggtga tacgcaactg catcggcacc gtggattcat taccggtgac
    13801 tgaggcattg gcatccgccc cttcaatcag catggctttc gcaaaacttc ctgacggaat
    13861 gtacggcagc gatggtccct gttttccctc attacgggta aacaccttac gctgtatccg
    13921 gttaggcacc ggcacagact ggtacgtcac ctgtggtggt ggcgtgacac cattcccggg
    13981 ataaaacgcc gtcggcggtg gaacagcaac gctgccctgc ggcgggaagg atacgggggt
    14041 gtttcctggc tgtggttcgc cttccggccc gggcggtgag gcaggcatct gaggtacagg
    14101 ctcaccggtc gccgtgacgg gattagcacc cagggctttt acctgctctg ccagggcggc
    14161 attgtcctgt cccagctttt caatacgacg ctggtcatca ccgcgctgtt tgttcaggac
    14221 atccagttca cgacggattt cctcatactg cttctgcatc tgcgctgccg tcacctgcat
    14281 ctctgtggtg gcatgctgac gcaccttgtc atcaaaggtc gtatccacca cacctgtcat
    14341 atccggcaca ggctcctgtt cagccaccgc ctcaccgtta ccggacatgt ccacatcaga
    14401 cagatacagt accccaccaa tcgcggaggc tgcaccgaca accacaatcc ccagccacag
    14461 gtactgcttg cgtttcacaa tggtattgat actggccatc agttgccctc cccgtcacgg
    14521 atgacataca catccatgcg cgcacctgcc agtaactggc tggcaggctg gctgaacatc
    14581 acggcacggg ttcccggctg ccagaagtca ctttcccgga tattcagggc agacagcgtt
    14641 ttattttcca cggcaaagcg gaccatcttc aggtgattcc ccgtccatac ggcatctgcc
    14701 actgaagaca gccccgccgg ggcctgcagg gtttcctttg tcacaggaac ctgataccag
    14761 cctgcaggta attttccgcc acggacagcc tggctgatgg tcacaagcag ggattcgtaa
    14821 ggcgtggacg tttcccacgc tccggcttct tctccggttc cgcgcaggtc actgaccagc
    14881 tgaatggtac gccccgcgcc ttcacgggga acagcctgaa tggaaaggtt cagaccacgc
    14941 tctgtttcca gaatgaacgt aaagggcttt ttgttgacgg tggccaccac cacaccgccg
    15001 gaggcggtct gctcattatt ggtcagggca ccatccagac tgttcacggc gataatccgg
    15061 tcaccgggaa cggtaaacag attcgggctg gtattgctga tggcaatact ggcctgacca
    15121 ccgttcacca ttggcaccac agtgggggcc agcgtaccgt ttgcggccat cacgctgcag
    15181 gaaaaaaaca ggccgccgaa tattattgcc gtattatttt ttctcatcgt ctgtttcccc
    15241 gaaattatcc agccaggtca ccccgttttc ccgcttcaga ataaggatgt aatgtttgat
    15301 atcagtaaaa ggttcggagt taccaatcca tgttttccga attccacgga tttccacacg
    15361 accatactga ggccagacac ggacactggt ctggaaaaaa gcagaattca cgttatcttt
    15421 tttaatgagt ttcgcttctt cagccagaat aactttcatc tggttctgtg cacccgggcg
    15481 gatatattgc agaagcgcct gatgtgaagc atcgacgatt tccggtgaga cattcagacg
    15541 gagggcaata aatgacagcg ccatctgctg caaataagag gcatcggcac tgtt
//
LOCUS       DAZOZD010000088        15582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOZD010000088 DAZOZD010000000
VERSION     DAZOZD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.088"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..2065
                     /locus_tag="ACHUPB_002229"
     CDS             <1..2065
                     /locus_tag="ACHUPB_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0759033.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="ACHUPB_002230"
     CDS             2081..3115
                     /locus_tag="ACHUPB_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR0759034.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHUPB_002231"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHUPB_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0759035.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHUPB_002232"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHUPB_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR0759036.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHUPB_002233"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHUPB_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="ACHUPB_002234"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="ACHUPB_002234"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0759037.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="ACHUPB_002235"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="ACHUPB_002235"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0759038.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="ACHUPB_002236"
     CDS             6844..7662
                     /locus_tag="ACHUPB_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR0759039.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="ACHUPB_002237"
     CDS             7714..8460
                     /locus_tag="ACHUPB_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0759040.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="ACHUPB_002238"
     CDS             complement(8434..9399)
                     /locus_tag="ACHUPB_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0759041.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="ACHUPB_002239"
     CDS             complement(9396..10400)
                     /locus_tag="ACHUPB_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR0759042.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="ACHUPB_002240"
     CDS             complement(10397..11674)
                     /locus_tag="ACHUPB_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR0759043.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHUPB_002241"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHUPB_002241"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0759044.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="ACHUPB_002242"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="ACHUPB_002242"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0759045.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHUPB_002243"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHUPB_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="ACHUPB_002244"
     CDS             15452..>15582
                     /locus_tag="ACHUPB_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0759046.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAZOZD010000089        15407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOZD010000089 DAZOZD010000000
VERSION     DAZOZD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.089"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHUPB_002245"
     CDS             <1..454
                     /locus_tag="ACHUPB_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0759047.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHUPB_002246"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHUPB_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUPB_002247"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHUPB_002247"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0759048.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUPB_002248"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHUPB_002248"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0759049.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUPB_002249"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHUPB_002249"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0759050.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUPB_002250"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHUPB_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0759051.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHUPB_002251"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHUPB_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0759052.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHUPB_002252"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHUPB_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0759053.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHUPB_002253"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHUPB_002253"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0759054.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHUPB_002254"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHUPB_002254"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0759055.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHUPB_002255"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHUPB_002255"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0759056.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHUPB_002256"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHUPB_002256"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0759057.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUPB_002257"
     CDS             10777..11529
                     /gene="gpmA"
                     /locus_tag="ACHUPB_002257"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0759058.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUPB_002258"
     CDS             complement(11688..12740)
                     /gene="aroG"
                     /locus_tag="ACHUPB_002258"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0759059.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13056..13436
                     /locus_tag="ACHUPB_002259"
     CDS             13056..13436
                     /locus_tag="ACHUPB_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0759060.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUPB_002260"
     CDS             13550..14491
                     /gene="zitB"
                     /locus_tag="ACHUPB_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0759061.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUPB_002261"
     CDS             complement(14488..15207)
                     /gene="pnuC"
                     /locus_tag="ACHUPB_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0759062.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15245..>15407)
                     /locus_tag="ACHUPB_002262"
     CDS             complement(15245..>15407)
                     /locus_tag="ACHUPB_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0759063.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4044 c         3851 g         3596 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat ataatgagaa
    10741 ttattatcat aaaaagataa tttgaggagt aagtatatgg ctgtaactaa gctggttctg
    10801 gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg gtacgacgtg
    10861 gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct gaaagaggaa
    10921 ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca taccctgtgg
    10981 aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg gaaactgaac
    11041 gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaaccgccga aaagtatggc
    11101 gacgagcagg tgaaacaatg gcgtcgtggt tttgccgtga ctccgccgga actgactaaa
    11161 gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga gaaagagctg
    11221 ccgctgactg aaagcctggc gctgaccatt gaccgcgtga tcccttactg gaatgaaact
    11281 attctaccgc gtatgaagag cggtgagcgc gtgatcatcg ctgcacacgg taactcttta
    11341 cgtgcgctgg tgaaatatct tgataacatg agtgaagaag agattcttga gctgaatatc
    11401 ccgaccggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct gaaacgctat
    11461 tatctgggta atgctgacga gatcgcagcg aaagcagcgg cggttgctaa ccagggtaaa
    11521 gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac tgcaagtgag tcggcttttt
    11581 tgttgctaac aatggagcac attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg
    11641 tgattcatcg gatacgccac tctgacggcg catccgacaa ttaaacctta cccgcgacgc
    11701 gcttttactg cattcgccag ttgacgtaac agagcatcgg tatcttccca gccaatgcag
    11761 gcatcggtga tgctcttacc gtaggccagc ggctccccgc tatcgaggct ctgattgcct
    11821 tccaccagat ggctttccac catcacgcca ataatggcct tttcgccacc ggcaatctgc
    11881 tggcaaacgt cagcacaaac atccatctgc tttttgaatt gtttggacga gttagcatgg
    11941 ctgaaatcga tcatcacctg tgctggcagg cctgctttgt tcagcccttc tttcacttca
    12001 gcaacgtgct tcgcgctgta gttaggctct ttaccgccgc gcagaatgat atggcaatca
    12061 ccgttaccgc tggtattcac aatcgccgaa tgcccccatt tagttacgga caggaagcag
    12121 tgcggcgcac cggcggcatt aatggcatcg atagccactt taatcgtacc gtctgtgcca
    12181 tttttaaaac ctaccggaca agaaagacca gacgccagtt cgcggtgcac ctgcgattcc
    12241 gtggtacgtg caccaattgc gccccagctc atcaggtcag cgagatactg aggagtgatc
    12301 atatcgagaa actcacccgc cgctggcaga ccgctgtcgt taatatcaag cagcaattta
    12361 cgggctatac gcagaccgtc gttgatctgg aagctgttat ccatatgcgg atcgttaatc
    12421 aaccctttcc agcccaccgt ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc
    12481 agctcatctt tcagctcttc acgcagcgcc agcaagcgag tggcatactc tttagccgcg
    12541 acaggatcat gaattgagca cgggccaatc accaccaaca ggcgatcatc attacctttc
    12601 aggatcttat ggatcgcttt tcgggcatgg gcaaccgtat tcgcggcatt ttcagttacg
    12661 gggaattttt ccagcaatgc gacaggagga agtaactctt tgatttcttt gatgcgtaaa
    12721 tcgtcgttct gataattcat gtctgttcca gtgttgccat acttatctta gtgaatgcaa
    12781 tacttccaat ctatatcttc cgtcagaatg tgtaaacggg gttttacact atgaacggat
    12841 taatcctgga attagcaaga aaaacgccag attgtcgcga aaaacgagat ctctcctaca
    12901 atttctaact gtaactcctt tgtttgttaa ttatttcaag attctctgct gcgtttcata
    12961 aactggctga aatcttaaac caatgcctta tattcacctg caaatgcact gttggaagag
    13021 gttatccgac ataacgacca taacaggagc atcctatgaa aatgacaaaa ctggccacat
    13081 tatttctgac tgccactcta agccttgcca gcggtgccgc actggctgcc gatagcggag
    13141 cgcaaactaa taacggccag gcaaacgccg cagctgatgc gggccaggta gcccctgacg
    13201 cccgtgaaaa tgtcgcgcca aataacgtcg acaataacgg ggtaaatacc ggttctggcg
    13261 gcacaatgct gcattcggat ggttcttcaa tgaacaatga cggaatgacc aaagatgaag
    13321 agcacaagaa caccatgtgc aaagatggtc gatgtccgga tatcaataaa aaggtacaaa
    13381 tcggtgatgg catcaataat gatgtcgata ccaaaaccga cggtaccacg cagtaaccct
    13441 gcaaaatagc caataatggg agagcggagt ctctcccttt taatttttca cttctaaaag
    13501 cagtatgatg tcataaagtt aagtgagaat aattaaggga tgactcgtca tggcgcactc
    13561 acactcacac acgtcctcac acctgccaga agataataat gctcgtcgct tgttgtatgc
    13621 tttcggcgtg actgccgggt ttatgctggt agaagtcgtt ggtggttttc tttctggttc
    13681 tctggcattg ctggccgatg cgggtcatat gttgaccgat actgccgccc tgcttttcgc
    13741 cctactcgcc gtgcaatttt cacgtcgtcc tcccaccatt cgccacactt tcggttggct
    13801 aagactgacc acgctcgcgg cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt
    13861 gattgtctgg gaggcgatag aacgcttccg cacgccgcgt ccggtcgagg gcggcatgat
    13921 gatggcaatt gccgtggctg ggttgctggc aaatatactt tctttctggt tacttcatca
    13981 cggcagcgaa gagaaaaacc tcaacgtacg agcagcggca ttgcatgtgc tcggggacct
    14041 gttgggttcg gttggggcta ttattgccgc gttgattatt atctggaccg gctggacacc
    14101 tgctgacccc attctctcga tagtggtgtc gcttctggtt ctgcgtagcg cgtggcgact
    14161 gttgaaagat agcgtgaatg aattacttga aggtgcaccg gtatcgctgg atatcgctga
    14221 actgaagcgt cgtatgtgcc gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt
    14281 atggatggta ggcgagaagc cggtgataac gctgcatgtg caggtgatcc cgccacacga
    14341 tcacgatgcc ttgttggatc agatccaaca ctacctgatg gatcactatc agattgagca
    14401 cgccaccatt cagatggaat atcaaccttg tcatgggcca gactgccacc tgaatgaagg
    14461 ggtgtctggc cattcacatc atcaccatta atgggacagc gcgcgtgagc ctctttcacg
    14521 tgcgctgttg atccacatcc gactgccgtt gagcgcaata aaggtcagga tgatgtactc
    14581 cagagacatg gcgtaaacgc cctgaagtgc aaaaataacg acgctaatca cgttaataat
    14641 cacccacaac agccagtttt ccacatactt acgcgtcatc agaatcattg ccacgataga
    14701 taacaccatc atgcatgaat cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac
    14761 aacctgtaat cctaatgctt gcatgatcat gactgccacg cgggtcaaaa atgcaaacac
    14821 cggattgata aataccgtca tcagaccaat cgaaacaacg caaaccgcca accagctgag
    14881 cgccttcggc aatggtaacc agcgaatttt caactccgcc tcgttctgac tggtttgtcg
    14941 cgaccacgca taccaaccgt aaatattcgc ggcaaagaaa aacacctgta atagcaggct
    15001 ggcatacagc tgaatctgaa agaaaataat gccaaacaag gtgacgttga tcaggccaaa
    15061 gaagtagttg ctgatcttct ccagactggc aaggccaata cacagcaacc cggcgatcgt
    15121 gcctaccgct tcgatccatg agagatcata accgcctgcc cctattggta tatgtaccag
    15181 gatattctgc acactaaaaa aatccatctt tcccccaaag cgtaacgtta cgccttatta
    15241 ttcgttatcc acgtagtgta gccgcaaaat ccagcatacg attgagcggc accagcgccc
    15301 tctctcgcag cctttcatca agaacctcgt gattgcttcc ttcctgttct aatgcctctg
    15361 cgatggactg aaggccattc atggccatcc acggacaatg cgcgcag
//
LOCUS       DAZOZD010000090        15337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOZD010000090 DAZOZD010000000
VERSION     DAZOZD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.090"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            99..851
                     /locus_tag="ACHUPB_002263"
                     /pseudo
     CDS             99..851
                     /locus_tag="ACHUPB_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(892..1824)
                     /locus_tag="ACHUPB_002264"
     CDS             complement(892..1824)
                     /locus_tag="ACHUPB_002264"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0759064.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2188..4974
                     /gene="polA"
                     /locus_tag="ACHUPB_002265"
     CDS             2188..4974
                     /gene="polA"
                     /locus_tag="ACHUPB_002265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0759065.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHUPB_002266"
     CDS             complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHUPB_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0759066.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6285..6451
                     /locus_tag="ACHUPB_002267"
                     /pseudo
     CDS             6285..6451
                     /locus_tag="ACHUPB_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6570..7079
                     /gene="yihI"
                     /locus_tag="ACHUPB_002268"
     CDS             6570..7079
                     /gene="yihI"
                     /locus_tag="ACHUPB_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0759067.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7268..8641
                     /gene="hemN"
                     /locus_tag="ACHUPB_002269"
     CDS             7268..8641
                     /gene="hemN"
                     /locus_tag="ACHUPB_002269"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0759068.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8831..8941)
                     /locus_tag="ACHUPB_002270"
     CDS             complement(8831..8941)
                     /locus_tag="ACHUPB_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0759069.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHUPB_002271"
     CDS             complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHUPB_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0759070.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHUPB_002272"
     CDS             complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHUPB_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0759071.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHUPB_002273"
     CDS             complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHUPB_002273"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0759072.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13479..15302
                     /gene="typA"
                     /locus_tag="ACHUPB_002274"
     CDS             13479..15302
                     /gene="typA"
                     /locus_tag="ACHUPB_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0759073.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4004 a         3829 c         3937 g         3567 t
ORIGIN      
        1 cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac
       61 accatcatac actaaatcag taagttggca gcatcaccag cgagtcgaaa tttaatcaga
      121 ttaacattat caatcacggg gaagacgttg cccaaatcga tgctttcgtt gcaaaaacca
      181 tgctggatcg cgttaaagac aaagattata tcaatgtcaa tctgacctac gaacttgata
      241 agttaacaaa agggaatcag caactcggta gtggtgagtg gtcattgatt gctgaatcta
      301 ttgatccctc agcagtgcgc caatttatca tccagtataa cattgctatg cagaagcagc
      361 ttgctgtaca ccctgagtta gcaaacgatg aagttgctct gcaagaagtg aatgctgcat
      421 tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc gaccattaaa caaccggtaa
      481 gatggaagaa cgcactcggc gaactaaatg ccaatctgga tatcagtatt gccgacccag
      541 ccaaatcttc atcatccaca aacaaagata tcaaattgct caattttgat gtgaagttac
      601 cgcttaatgt cgtcacagaa accgcaaaac agcttaattt atctgaagga atggatgcgg
      661 aaaaagcgca aaagcaggct gataaacaaa tcagcgggat gatgacattg ggtcagatgt
      721 ttcagttaat cacgattgac aacaataccg cctcgctgca gctgcgttat acaccgggta
      781 aagttgtgtt taacggacag gagatgagcg aagaagaatt tatgtctcgt gccggacgtt
      841 ttgttcatta aaatactcac tcgcctctgc tcattcagag gcgagtaaaa cttacttatt
      901 ctgacgctgt gatgacatga gagacgtcag aagccggtct ttttcctgcc acaatgagtt
      961 cagccattgc tgaaaatggc gcttaaagct tttgtcattg atgtagtcgc catgtaactc
     1021 atcggcaata ggctgtaaat ccacatgtac gacaatccgc gtcagtttac cgcttaacat
     1081 atcgaagaac ggctgacggt tattgtccgg ataacacagc gtaacattaa gcagtttatc
     1141 gaattgttta cccagtacat ttagcgccat cgcaatgcct gccgcctttg gtggcaacaa
     1201 gttttgaaaa gtggagtggg tttgctgatg tttttcttgc gtgaaacggg agccttcaac
     1261 gaaattgaca atggtggtgg gatgcaggcg aaacttttca caagaacgcc gagtggtttc
     1321 aacatcttta ccacgtcgct ccggatgacg taataaataa gcgcgggaat aacgcttcat
     1381 aaacggcata tccagcgccc agcacgccag gccaagaaat ggcacccagg ccagctgttg
     1441 cttgaggaaa tatttattca tcggaatgtg cttacgaaac agcacgcaca gtacgacaat
     1501 atctgcccaa ctacggtggt tacaaataag cagataccag ttcttcttac ttaacccttc
     1561 cagcccgtga acttcccatt gcaggtgtgg gttcagatgc agtaataccg ccagaccttc
     1621 acaccagcaa tacatcataa aatcacaaaa acgcgaaacc tttcgccaaa tgactggcac
     1681 aggtagcaat agttttacaa tcccggcaat gatgatcggg acggaacaaa aaatagtgac
     1741 caaaatggtc aatacgatac tcagcaaaag ggttatcgca gcgagtattc tcgtcataat
     1801 gaatttattc aaaaggttag ccatagacag tgcgctaaag aagcgcaagg cgcagatttt
     1861 agcagaaaac gacccaaata acggatgatc cttaaggaga aaaataatcc atatctatcc
     1921 acattagaaa aaatcccatt atctcaatta ttagggatgg ctttattttt aactgcatga
     1981 aaaacaaaga caaacatcat gctgtaaaaa gcatgataat aaattaaaag cgatgtaaat
     2041 aatttatgca caaagttatc cacatgacga tttgcgagcg atccagaaga tctacaaaag
     2101 attttcacga aaagcggtga aaaactcatg ttttcatcct gtctgtggca tcctttaccc
     2161 ataatctgat aaacaggcac ggacattatg gttcagatcc cccaaaatcc acttatcctt
     2221 gtagatggtt catcttatct ttatcgcgca tatcacgcgt ttcccccgct gactaacagc
     2281 gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca tgctgcgcag tctgatcatg
     2341 caatataaac cgacgcatgc agcggtggtc tttgacgcca agggaaaaac ctttcgtgat
     2401 gaactgtttg aacattacaa atcacatcgc ccgccaatgc cggacgatct gcgagcacaa
     2461 atcgaacccc tgcacgcgat ggttaaagcg atgggactgc cgctgctggc ggtttctggc
     2521 gtagaagcgg acgacgttat cggtactctg gcgcgcgaag ccgaaaaagc cgggcgtccg
     2581 gtgctgatca gcactggcga taaagatatg gcgcagctgg tgacgccaaa tattacgctt
     2641 atcaacacca tgacgaatac catcctcgga ccggaagagg tggtgaataa gtacggcgtg
     2701 ccgccagaac tgatcatcga tttcctggcg ctgatgggtg actcctccga taacattcct
     2761 ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc tgcaaggtct tggcgggctg
     2821 gatacgctgt atgccgagcc agaaaaaatt gctgggttga gcttccgtgg cgcgaaaaca
     2881 atggcagcga agctcgagca aaacaaagaa gttgcttatc tctcatacca gctggcgacg
     2941 attaaaaccg acgttgaact ggagctgacc tgtgaacaac tggaagtgca gcaaccggca
     3001 gcggaagagt tgttggggct gttcaaaaag tatgagttca aacgctggac tgctgatgtc
     3061 gaagcgggca aatggttaca ggccaaaggg gcaaaaccag ccgcgaagcc gcaggaaacc
     3121 agtgttgcag acgaagcgcc agaagtgacg gcaacggtga tttcttatga caactacgtc
     3181 accatccttg atgaagaaac actgaaagcg tggattgcga agctggaaaa agcgccggta
     3241 tttgcatttg ataccgaaac cgacagcctt gataacatct ctgctaacct ggtcgggctt
     3301 tcttttgcta tcgagccagg cgtagcggca tatattccgg ttgctcatga ttatcttgat
     3361 gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc taaaaccgct gctggaagat
     3421 gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc gcggtattct ggcgaattac
     3481 ggcattgagc tgcgtgggat tgcgtttgat accatgctgg agtcctacat tctcaatagc
     3541 gttgccgggc gtcacgatat ggacagcctc gcggaacgtt ggttgaagca caaaactatc
     3601 acttttgaag agattgcggg taaaggcaaa aatcaactga cctttaacca gattgccctc
     3661 gaagaggccg gacgttacgc cgccgaagat gcggatgtca ccttgcagtt gcatctgaaa
     3721 atgtggccag atctgcaaaa acacaaaggg ccgttgaacg tcttcgagaa tatcgaaatg
     3781 ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg tgaagatcga tccgaaagtg
     3841 ctgcacaatc attctgaaga gctcaccctt cgtctggctg agctggaaaa gaaagcgcat
     3901 gaaattgcag gtgaggaatt taacctttct tccaccaagc agttacaaac cattctgttt
     3961 gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg gcgcgccgtc aacgtcggaa
     4021 gaggtactgg aagaactggc gctggactat ccgttgccaa aagtgattct ggagtatcgt
     4081 ggtctggcga agctgaaatc gacctacacc gacaagctgc cgctgatgat caacccgaaa
     4141 accgggcgtg tgcatacctc ttatcaccag gcagtaactg caacgggacg tttatcgtca
     4201 accgatccta acctgcaaaa cattccggtg cgtaacgaag aaggtcgtcg tatccgccag
     4261 gcgtttattg cgccagagga ttatgtgatt gtctcagcgg actactcgca gattgaactg
     4321 cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga ccgcattcgc ggaaggaaaa
     4381 gatatccacc gggcaacggc ggcagaagtg tttggtttgc cactggaaac cgtcaccagc
     4441 gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga tttatggcat gagtgctttc
     4501 ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc agaagtacat ggacctttac
     4561 ttcgaacgct accctggcgt gctggagtat atggaacgca cccgtgctca ggcgaaagag
     4621 cagggctacg ttgaaacgct ggacggacgc cgtctgtatc tgccggatat caaatccagc
     4681 aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta acgcgccaat gcagggaacc
     4741 gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg cgtggttaca ggctgagcaa
     4801 ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg tatttgaagt tcataaagat
     4861 gatgttgatg ccgtcgcgaa gcagattcat caactgatgg aaaactgtac ccgtctggat
     4921 gtgccgttgc tggtggaagt ggggagtggc gaaaactggg atcaggcgca ctaagattcg
     4981 cctgaacatg cccttttttc gtaagtaagc aacataagct gtcacgtttt gtgatggcta
     5041 ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa agtgctttct gaactgaaca
     5101 aaaaagagta aagttagtcg cgtagggtac agaggtaaga tgttctatct ttcagacctt
     5161 ttacttcacg taatcggatt tggctgaata ttttagccgc cccagtcagt aatgactggg
     5221 gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg atgcgctatg tttatcaggc
     5281 caacggtaga attgtaatct attgaattta cgggccggat acgccacatc cggcacaagc
     5341 attaaggcaa gaaaattatt cgccgtcctg cgtttcttct acaggctgca tctcgctaaa
     5401 ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt ttcttcaacg aagaaaacgt
     5461 ttcaacctgc acatcaccgt taaacgccag tacagcttca cgcaccatat tcaattgcgc
     5521 tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc agcaccagaa cggcgatatt
     5581 gctgtctacc gcccactcaa tcatctgctg atccaaatct ttcagcggat ggcgaatatc
     5641 cattagcacc accagacctt gcaggctctg acgtttttcg aggtattcgc cgagcgcacg
     5701 ctgccatttg cgcttcatct cttccgggac ttccgcataa ccgtacccag gcaagtcaac
     5761 cagacgcttg ccgtcagcca cttcaaacag gttgataagc tgggtgcgcc ctggggtttt
     5821 tgaggtacga gccaggcttt tctggttagt cagcgtgttc agcgcgctgg atttacctgc
     5881 gttggaacgg cctgcaaaag ccacttcaat tccggtatcg gaaggtaggt ggcgaatatc
     5941 aggcgcactc atcacaaaat gcgtctgttg ataattcaaa ttagtcaaag cggtcgtctc
     6001 cgtcagtcaa agctttgtgg cgattatacc tgaaccataa taaaaggcgg atttttcggc
     6061 gtgagcgttg taagtaaaag ccatacgctt tgtgagacat tgccgatagt ctttatgcga
     6121 aatagcagag aaaattctgc gatgcatgtc aaataagcta tataaatcag tgaattgctt
     6181 ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt tgattgtttg tttaaagcaa
     6241 aggcgtaaag tagcacccat agagcgagga cgctaacagg aacaatgact caggatgagg
     6301 gtcaggagcg ccaggaggcg aagacagagg attgtcagga agacaaacgt ccggagacgt
     6361 aattaaacgg aaatggaatc aacacggatt gttccggcta aaggaaaaac agggtgtgtt
     6421 ggcggcctgc aaggattgta agacccgtta agggttatga gtcaggaaaa aaggcgacag
     6481 agtaatctgt cgcctttttt ctttgcttgc tttctgttag attccgccgc aaatctatac
     6541 tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc atcttcaaac tcacgcagca
     6601 aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct ggatcaggaa gctcgtgatc
     6661 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggaaaca
     6721 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
     6781 ctattccatt gggcgtggct gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
     6841 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
     6901 tgctggaacg tctggaagca ggcgaaaccc tgagcgccga agagcaatcc tgggtggatg
     6961 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
     7021 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggtaactaac
     7081 ggattgccac cgtgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
     7141 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
     7201 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
     7261 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
     7321 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
     7381 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
     7441 tatcccgttc tgccataagc tttgttactt ctgcggttgc aataagattg ttactcgcca
     7501 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
     7561 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
     7621 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
     7681 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
     7741 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
     7801 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
     7861 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
     7921 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
     7981 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
     8041 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
     8101 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
     8161 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
     8221 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
     8281 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgctctgtg
     8341 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
     8401 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
     8461 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
     8521 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
     8581 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
     8641 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
     8701 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
     8761 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
     8821 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
     8881 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
     8941 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
     9001 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
     9061 tccccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
     9121 cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt cagtaacgtc cgctccagct
     9181 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
     9241 gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
     9301 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
     9361 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
     9421 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg cgcgcggcaa
     9481 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
     9541 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
     9601 ctcgctgttc gagattctgg tgagtggcag cgataatacg cacatccact ttcaccggcg
     9661 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
     9721 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
     9781 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
     9841 ctgattcgat caaatctttt gggatagctg ccatattcag cgcgataaac ggcgctttgg
     9901 cgcgcggact gtggcgatgc agggcatgag cgaccagttc tttaccggtg ccggattcgc
     9961 cgttaatcag cacgctaata gaagaacgcg aaagccgacc gataatacgg aacacgtcct
    10021 gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc gttaagctga atattacgcg
    10081 gctgctgctg ttcctggtaa tgactgatag cgcgctcaac cagcgccact gcttcgtcga
    10141 tatcaaacgg tttgggcaga taatcaaacg ccccttgttg ataggcgctg acggcagcat
    10201 ccagatcgga atgtgcggtc ataatgatga ccggaagcat tggatggcgc tgtttaatct
    10261 gcttgagcag cgccagcccg tccattcccg gcatacggat atctgaaagc agcacatccg
    10321 gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt ctcaaatgtc gtacaggtta
    10381 aacccgctcc agcgagcgca cgttcaagca cccaacggat ggaactatcg tcatcgacta
    10441 cccagactat ccctcgttgc ataaacgtca cctttatttc ctgataggca ggtaaaccga
    10501 gaactcggta tgacctggcc aactggtaaa ttcaattttg cctgaatgct gatcaatcaa
    10561 attacgggcg atggataagc caagcccggt gccaccttcg cggccgctga ccatcgggta
    10621 aaacagcgta tcctgcaaat gaggcggaat gcccggcccg ttatcttcca catcaatccg
    10681 cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga aacgcggtgc gggtacgcag
    10741 aatgatttca ccgccttccg gccccagcgc ctgtagcgca ttgcgcacaa tattcagcaa
    10801 gacctgttca atttgatccg ggtcgtgcgc cagttccggc aggctggggt cgtaatcacg
    10861 aatcaaccgc acgttgtccg gcagttccat cgacaccagc gtcaccacgc gttcagctac
    10921 tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc ggccccaaca gacggtcgac
    10981 cagatttcgc agccggtccg cctgttcgat aatcactttg gtatattcga gtagtgatgg
    11041 gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt aaacctccaa gcggattttt
    11101 aatctcatgt gccaggccgc gcactaaatc acgggcagca acctgctggg cgtgctgtag
    11161 ctgttcctga cttaagcggc gctggttatc catcggagcc atctccagca ggatcatgcc
    11221 gtccggcata cgctgggccg tcacagaaag gatatgcgag cgcccgtcga tgaccagcgt
    11281 cacttcgtta tcggtaaaac cttgccccgc ctccagactt tcttgcatca gctcgatatt
    11341 taatgagaag tagctcaaca gttccggtaa cggtgtacca aacaatttgc gggagctttg
    11401 ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc gccagattgt catcaattaa
    11461 caaaatactg ttaatcagcg agttgaggat ctgcccagca tcgggctgcg tgcctgttgc
    11521 cataaagcag tctcctgaac aggttgcacc attttagtgc attatagctt tttacggata
    11581 aaaagcgcga agcatcagag aattgacgga gaaaaaagcc catgcagaga tgggctgaaa
    11641 gtttccacgg caactaaatc ccggcgttgt tgcgccgggt agtacaactt caactattag
    11701 acgctgtagt acagctcaaa ctctaccgga tgcggagtca tacgcacgcg gtcatcttct
    11761 tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag tgaacacgcc accggctttc
    11821 aggaactcgc ggtccagatc cagttcgttc agtgcttctt ccagagagcc tgcaacctgt
    11881 gggatctctt tcgcttcttc tggcggcagg tcatacaggt ttttgtccat ggcttcgccc
    11941 ggatggatct tgttcttgat accatcaaga ccggccatca gcagggcagc aaagcacagg
    12001 tacgggttag ctgccggatc cgggaaacgt acttcgatac gacgtgcttt cggagaagaa
    12061 accaccggaa tacggataga cgcagaacgg ttacgcgcag agtaagccag cattaccggt
    12121 gcttcatagc ccgggaccag acgcttataa gagttggtgg tcgggtttgc cagggcgtta
    12181 atcgctttag cgtgtttgat tacgccgcca atgtagtaca gcgcctgctc agacagacct
    12241 gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag acagagacat gtggcagtgc
    12301 ataccggagc cgttatcacc gaacatcggt tttggcataa aggtcgcggt tttaccgaag
    12361 cggtgcgcta cgttgtgcac aacatatttg tagatctgaa tttcgtcagc ttttttggtc
    12421 atagtattga agcgggtagc cacttcgttc tgaccagcag tcgctacttc gtggtgatgg
    12481 gcttcaacca ccagacccat ctgttccatc accagacaca tttcagaacg aatatcctga
    12541 gccgagtcta ccggtggaac cgggaagtaa ccgcctttca ctgccggacg gtgacctttg
    12601 ttaccacctt cgtattgggt ggaggagttc catgcgcctt cgatatcgtc gatagcaacg
    12661 tgggaaccgg agatagatga tccgaaacgg atgtcatcga acaggaagaa ttcaggttct
    12721 ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca ggtaatcttc ggcgcgcttc
    12781 gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc caggttcaag gatgtcgcaa
    12841 cggataatca gggtggagtc ggcgaagaac gggtcaatca ctgcggtgga tgcgtctggc
    12901 atcagcacca tgtcggactc gttaatgcct ttccagccgc caatcgagga gccgtcaaac
    12961 attttgcctt cttcgaagaa ttcagcattc acctgatgag cagggatagt gacgtgctgt
    13021 tctttacctt tagtatcggt gaagcgcaaa tcaacaaact tcacttcgtg ctcgttcagc
    13081 atcgtcagta cgtgttcagc ggacatactt taactctcct ggattggtca tggtcgtcgt
    13141 ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa aaaagataaa gcgaaatctg
    13201 tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg caccatcgtg caaaagggct
    13261 gcaccacgat gtgaatgttg caccaatata gtgcttcaat ggaaacatta agcaccatgt
    13321 tggtgcaatg acctttggat aacccttttt atgctccgtg aaagcgatca caaagggact
    13381 ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt tgtagtgcga ttaatccgtg
    13441 tacaataacg cgctatttct aatgcctgag gcaaagttgt gatcgaaaaa ttgcgtaata
    13501 tcgccatcat cgcgcacgta gaccatggta aaaccaccct ggtagacaag ctgctccaac
    13561 aatccggtac gttcgactct cgtgccgaaa cccaagagcg cgtgatggac tccaacgatt
    13621 tggagaaaga gcgtgggatt accatcctcg cgaaaaacac cgctatcaaa tggaatgatt
    13681 accgtatcaa catcgttgat accccggggc acgccgactt cggtggtgaa gttgaacgtg
    13741 taatgtccat ggtagactca gtgctgctgg tggttgacgc atttgacggc ccgatgccgc
    13801 aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct gaagccgatt gttgttatca
    13861 acaaagttga ccgccctggc gcgcgtcctg attgggttgt ggatcaggta ttcgatctgt
    13921 tcgttaacct cgacgcgacc gacgagcagc tggacttccc gatcgtttac gcttctgcgc
    13981 tgaacggtat cgcgggtctg gaccacgaag atatggcgga agacatgacc ccgctgtacc
    14041 aggcgattgt tgaccacgtt cctgcgccgg acgttgacct tgacggtccg ttccagatgc
    14101 agatttctca gctcgattac aacagctatg ttggcgttat cggcattggc cgcatcaagc
    14161 gcggtaaagt gaagccgaac cagcaggtca ctatcatcga tagcgaaggc aaaacccgca
    14221 acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga acgtatcgaa accgatctgg
    14281 cggaagctgg cgatatcgtg gcgatcacgg gccttggcga actgaacatt tctgacaccg
    14341 tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc cgttgatgag ccgaccgttt
    14401 ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa agaaggtaag ttcgtaacgt
    14461 ctcgtcagat cctggatcgt ctgaacaaag aactggtaca caacgttgcg ctgcgcgtag
    14521 aagaaaccga agacgccgat gcgttccgcg tttctggtcg tggcgaactg cacctgtctg
    14581 ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc ggtatcccgt ccaaaagtaa
    14641 tcttccgtga aatcgacggt cgtaaacaag agccgtatga aaacgtgact ctggacgttg
    14701 aagaacagca tcagggttct gtaatgcagg cgctgggcga acgtaaaggc gacctgaaaa
    14761 acatgaatcc agacggtaaa ggccgcgtac gtctcgacta cgtgatccca agccgtggtc
    14821 tgattggctt ccgttctgag ttcatgacca tgacttccgg tactggtctg ctgtactcca
    14881 ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg tcagcgtcag aacggcgtac
    14941 tgatctctaa cggtcagggt aaagcggtcg cgttcgcact gttcggtctg caggaccgcg
    15001 gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg tcagattatc ggtattcaca
    15061 gccgctctaa cgacctgact gtaaactgcc tgaccggtaa gaaactgacc aacatgcgtg
    15121 cttccggtac tgacgaagct gttgttctgg ttccgcctat ccgcatgact ctggaacaag
    15181 ctctggagtt catcgatgat gacgaactgg tagaagtgac tccgacctct atccgtattc
    15241 gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa ccgcgcaccg aaagacgatt
    15301 aatttcgttt tttagttata aaaaacctgc catcgct
//
LOCUS       DAZOZD010000091        15205 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOZD010000091 DAZOZD010000000
VERSION     DAZOZD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.091"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..350
                     /locus_tag="ACHUPB_002275"
     CDS             <1..350
                     /locus_tag="ACHUPB_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0759074.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
     gene            complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHUPB_002276"
     CDS             complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHUPB_002276"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR0759075.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(1733..2386)
                     /locus_tag="ACHUPB_002277"
     CDS             complement(1733..2386)
                     /locus_tag="ACHUPB_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0759076.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2386..3922)
                     /locus_tag="ACHUPB_002278"
                     /pseudo
     CDS             complement(2386..3922)
                     /locus_tag="ACHUPB_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            complement(3895..5061)
                     /locus_tag="ACHUPB_002279"
     CDS             complement(3895..5061)
                     /locus_tag="ACHUPB_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0759077.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(5071..5619)
                     /locus_tag="ACHUPB_002280"
     CDS             complement(5071..5619)
                     /locus_tag="ACHUPB_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0759078.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(5619..6326)
                     /locus_tag="ACHUPB_002281"
     CDS             complement(5619..6326)
                     /locus_tag="ACHUPB_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0759079.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHUPB_002282"
     CDS             complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHUPB_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0759080.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(7023..7733)
                     /locus_tag="ACHUPB_002283"
     CDS             complement(7023..7733)
                     /locus_tag="ACHUPB_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0759081.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHUPB_002284"
     CDS             complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHUPB_002284"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0759082.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            9152..10372
                     /gene="astC"
                     /locus_tag="ACHUPB_002285"
     CDS             9152..10372
                     /gene="astC"
                     /locus_tag="ACHUPB_002285"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR0759083.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10369..11403
                     /gene="astA"
                     /locus_tag="ACHUPB_002286"
     CDS             10369..11403
                     /gene="astA"
                     /locus_tag="ACHUPB_002286"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0759084.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            11400..12878
                     /gene="astD"
                     /locus_tag="ACHUPB_002287"
                     /pseudo
     CDS             11400..12878
                     /gene="astD"
                     /locus_tag="ACHUPB_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            12875..14218
                     /gene="astB"
                     /locus_tag="ACHUPB_002288"
     CDS             12875..14218
                     /gene="astB"
                     /locus_tag="ACHUPB_002288"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR0759085.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            14211..15179
                     /gene="astE"
                     /locus_tag="ACHUPB_002289"
     CDS             14211..15179
                     /gene="astE"
                     /locus_tag="ACHUPB_002289"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0759086.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         3520 a         3981 c         4193 g         3511 t
ORIGIN      
        1 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
       61 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
      121 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
      181 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
      241 ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
      301 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
      361 atttgctact gtccgggccg cgttcaccgg ttgctaccgg attttttgga tcgctgctcc
      421 attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca
      481 taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgctctggtt
      541 tgatattcca cgctttccac atagcggtaa tatcatcggc gctgcgcatg gtgccatccg
      601 ggttatgaaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg
      661 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
      721 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca
      781 tatcgagcat caactgcggt tgtgccggga gcttcacgcc gaaatccggt tccgctttca
      841 ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc
      901 catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc
      961 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg
     1021 ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
     1081 cgttggtatc gatgtagtca gcaccgggaa tatggctgat aaggtaaagc ttaggagcgc
     1141 cccaggccgc ttcaatgact ttccagtcac cggcaggttt cgccgtaacc tctttacctt
     1201 gttgcaggtc gtgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta
     1261 gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt
     1321 gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc gtacagcgcc accggggcat
     1381 cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcga
     1441 gccagctggc agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt
     1501 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgtt
     1561 gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
     1621 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
     1681 ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca
     1741 gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat
     1801 cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ttcgcgaact tcgctgaaca
     1861 cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga
     1921 gtaacgcttt tggctgagcg agaagggcgc gcagcagggc cacccgcgct cgctgaccgc
     1981 ctgataatgt cgcagggtcc tggtgataag tttcagccag tcccgctcga tcaagcgcgt
     2041 ctttcacggc attgcgcctg gcagtcccct tgagcgtcga cagtagcgcc agcagtaaat
     2101 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg gaaaagaatg ccaatctgac
     2161 gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag
     2221 aaaactgtcc agccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag
     2281 acggccccat taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca
     2341 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggagtccttg
     2401 tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa
     2461 caacagttgc cataaagcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
     2521 caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
     2581 ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcacagggc gcaacatgag
     2641 tgggcatttc acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
     2701 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
     2761 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatccaggt tcagccatag
     2821 cgccagcgtg tactggcccg ccaccagtgg cagatcgggc aatagaatgg gcaaccatag
     2881 ccacaactgg cgacgctgtg gcccccattc cagccacagc agtaacagga gcaaagcgat
     2941 gaatgttgct accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga
     3001 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
     3061 cgccagcgta gtgcctggta ataaaggtgt ggcaggcatt gcgaatgcca tctacgcggg
     3121 gaatagtgcg ccgccagctg cgccatagca gatagctcag caaaacgtag gcggcgagta
     3181 acagcatcag cagcagactg gcgagtgcgc ctttcgtttg ttgatccgcg tcgccctgag
     3241 ttaaccactg ccagctaatt actgccagcg tcggtggatt acctggccca agaataatcg
     3301 ccacatccac gaccgaaagc gaccaggcaa caatcgccag catcgccatt gccagcgaag
     3361 gcgcgacgga gggcaacagc agccagttca ggcattgcca gcggctgtag cccagcgaat
     3421 ccagcacaat gacctgctgc aacagccgtt tttcgctcaa tactgccgct aagatccaca
     3481 gcagaaatgc gctttctttc actgcaaggg tgaggcccag accgatgcca aatcggtcca
     3541 ttggcggagt gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca
     3601 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca
     3661 tacgctgcca tttcggccca ggctatagcg ccacaatcac cagcagggca atcaacaatg
     3721 ccccgaccgc cgcgatggtt gtcgatacca gcgttgccag taatgcatgc ggtaactgtg
     3781 gatcggcaaa taacgcctgc cagtgtgtca acgacaacgc aggcgatatc agcgtgagag
     3841 ctgccgggat cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     3901 cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc
     3961 agccagtacc ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc
     4021 aggcagtttt tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg
     4081 cagttgcgca tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt
     4141 cgcgttggct ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa
     4201 actgtaactg cttgccggca aatcgccgct ggcgattttt tgctgcgcat gcgcggggtt
     4261 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     4321 aggcgggaaa tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcca
     4381 caagggcgca gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg
     4441 atctggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     4501 gcgcggataa gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     4561 ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca
     4621 gggcgattcc gcaccttgtg tcggcactga aaaatcttcc cgcaccggca gctgtgtgtc
     4681 gacatagcgc cagttgggca gagtctccgc ccagcccgtt tgtaataaat tggcctcttt
     4741 taaggtgcgg aagttttcgc cgttcaccca gagcagatcc accgagccgc ccgttttacg
     4801 accggcagca gcttcggtct gaatgcgctt caccgcgtct gcggcatccg cgagacgaac
     4861 aatcttcagg tttatagcgt aatgggtttt catctcgccg ctaacccagt cgagatagcg
     4921 gttaattgcg gtatcgccgc cccaggcgtt aaaccagacg gtttgccctt tggcctcatt
     4981 tttaatttcc tgccagtcac tggcatgtgt tgccaaagaa aacagcgata acaatcccag
     5041 caaccaccca caatggcgca tcttctctcc ttaagaacgt ttttccggaa tacggcacag
     5101 cccctgagcg ggaatgtcca tggcagaatt aaaacgagcc gacaggtttt gcaggccgat
     5161 caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc gcagccagtc gggtacgcag
     5221 ggcatcatcg actgttggcg gtgtgacgct tgcggcttcg gcgtactcca gcgccagccg
     5281 ttcttcatcg ctaaagagcg ggttttgccg ccagtcagcc actgccagca gtttatcgct
     5341 gctgccggta cgctcggcga ctttcatact ggtgatatcc acacaaaact cacacaggca
     5401 catttgcgca atgcgcgcgc tgacaagcga tcgcactacc ggatcgagcg gtgagcgttt
     5461 gcgctccagc cacccaacaa acattgacac caaataaaag ataaacggga tccgccccca
     5521 ccagcgaatc gggcttagca cctcgccgta atggcgacga tgcagccacg cctgcggacg
     5581 taaaatgaaa ggaattttgc taagcggcgg taaacccatc agtcattcct cttctggcgc
     5641 tcaagccata ttttcttcgc cataaaaatc accacggtca gggcaaacag aataaacagt
     5701 ccggtgacaa accagaaagt accgcctgtt aacatgctgc ccgcccagga ataaacaata
     5761 gtcgtcggta attgaccaag cccggtggcg ataaaaaacg agcgaaaacg tattgaagtc
     5821 agatcggcag catagctgat tggatcgaaa gggacaaaag gcaataaccg acagaccagg
     5881 atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac tgtcaagcac ggttttgccg
     5941 gttaattttt ccaccacttc gcggcccatc actctggcga taaaaaagca cagcgccgcg
     6001 ccggccatcg aactggtcca cgacagcaag ccgccccaga acgcgccaaa cagcgacgca
     6061 ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa tggcctgtaa aatcatcaac
     6121 aagaacgaga caactgccgc cagtgcgccg taagactgaa taaagcgttc tataccctgt
     6181 tggtccacgg cggcaaacgc cgcaacgctg cgattgataa actcatggac gccgggaagc
     6241 agcgcccatg cgagcagcat agcgaacaac aggatgatga gggttatacg gtagtaccag
     6301 attttacgcg attgcatctt catcatcctt tatcctggct ttattcggtg attttaaagg
     6361 taaccgttgc cagcgtgtta tccgccggga gaactgaggc attgcctttg aatttcactt
     6421 cgccgcgttt ttccaccgca ccataagtgt atgttgcatt gctgacgatg ccgaccgggc
     6481 agctatccag acacaccagg caacccgttt tcttctcttc tgctgccgtt aaattaccgc
     6541 caaagcgcat gtccagtttc ttgccattac tgtcaacaat cacttcatcg aaggaatacg
     6601 cttttgccgc cccttgccag ttgaccgaaa tatccagttt gctgcctgtg acatgagtcg
     6661 tttctttatt atccatcgtc atgttttcgc ccggcgtacc acctgcctct ttcagggctt
     6721 cataaaatgc tttcggggtc gcataagcca taaacagcga tttatgtccg ttggagccat
     6781 ctttaaacac aataccgtga cgagtgtcgt cggtgaggaa acgaccatta atttgcacca
     6841 acatggttac cgtatggtta ttggcatcga cgcgcagcgg ttggctgttc gaaagtccgt
     6901 cgtattccac ttttgccgcc gcattctctt tttgatcgca gccgctaagc ccggcaacag
     6961 ccagtaagca gagtgcagcc agtccttttt tccatgacac tgaataatgt tgcgacatcg
     7021 ttctatcctt cattttttgg gtgagtaagc gggctgcggc gcgaagcaga cagatccaca
     7081 tgtttgtgtc gggcgtagag ttttgcgagc tggacgagaa taaacagcgc caggcccgcc
     7141 agacagagtt gtaaaataaa gcgcagcgta atgccttcat tggcgagatc gcttgccatc
     7201 acggtataaa taacaatacc gggcagggtc gtgagtgccg aaataagggt ataaggccag
     7261 aaggcgatgg tggttaatcc gtaagcgtaa ttttgaatat tgtaaggaaa caacgggatt
     7321 aagcgggtca gaataagaaa atcaataccg ttacgcgcaa tgcctttttc aatggcctgg
     7381 aaggtatggc tatggcccac gtatttcagc agtaaatcac gtcccagcca ccgcgccagc
     7441 aggaatgagc acgaggaggc cagcgtggcg gcaattaatg agagtagtgt ccctaagagc
     7501 gggccaaaaa ctattcctcc agcgatcacc agtatgcttc ctggtaacac aaagagggtg
     7561 gcaatgatga ataacagaat atagaggcta taaccgaaca atccgctctg gcgaatgagt
     7621 gtctgtaagt ggggtaaatc ggtgagcaga tcgaataaac cgaaagcgtg gatagcgtaa
     7681 atgaccagcg caaaaataag acaggcgaaa agaaatttac gctcagcatt catcgtggag
     7741 tttcctatat caaacttgat aatagagaag tggtcagaca actaaaaacg cagtgacggt
     7801 ataacaaagg acggcaggca acaaatcatt actgacagtt tgttgatttt tctcaaggtt
     7861 aattctcctg acccagtttg agccaggaga gctgctaaat tagcggcgga aggtcgccca
     7921 gacgggggcg tgatcggacg gtttttccat gctgcgaatt tcatagtcga tgccggtttc
     7981 tacgcaacat tctgccagcg gttggctggc gagcagcagg tcgatgcgca gaccacggtt
     8041 atcgtcaaaa ccttttgagc ggtaatcaaa ccatgagaaa cgatctgctg tttgcggatt
     8101 agcatggcgg aaggtatcga ccaaccccca gctcatcagc ctgtccatcc attcgcgctc
     8161 ttccggcagg aaagagcatt taccggtacg cagccagcgc ttacggtttt cttcgccaat
     8221 gccgatatcc agatctgtag ggctgatatt catatcgccc ataatcagta ccggattatc
     8281 acgtttgagt tcggtttcca ggtagttttg cagattctga taaaactgcg cttttgccgg
     8341 gaattttatc ggatggtcgc ggctttcacc ctgcgggaag taaccgttga tcacggtgac
     8401 attacccagc agtgagggga tttccgccat aataatccgc cgctgcgcct cttcgtcgtc
     8461 accgggaaag ccgcgacgca cggcaatcgg cgtctctttg gtcagcagcg ccacgccata
     8521 atggcctttc tgcccgtgat aaaacacgtt gtagccgagc ttcgccacct cttcgagcgg
     8581 aaacatatcg tcatgaactt ttgtctcctg caggccaatc acatccggtt ggtgcttttc
     8641 gacgatggct tcaagctggt gaggtctggc gcgcaggccg ttgatattaa aagagacaaa
     8701 tttcatagtc gctgccattt attcagataa agagtgcgtg gatggtagca gaatttcgcg
     8761 ttgcttaccg cctgttgtta agtgatttga tcgatggttg ttaatgatgt caacgatggc
     8821 gcaaaaaatg cccgcttttg gtgcgcgctg cgtcagaatg gcgcagtaat ttccagtaaa
     8881 tttcgtcaat gatcacaatt ccagaattat attttacttc tgcataacat tgcgtttttt
     8941 attcttttta ttcacccaca tcgcaaacgt attcacttta tatgcacttt aaatgcatat
     9001 ggtttggtta taactccttg atttccagtt agcctccgcc gtttatgcac ttttatcact
     9061 ggctggcacg aaccctgcaa tctacattta cagcgcaaac attacttatt gttaacatat
     9121 agatagcgaa ttatttactg tagaggtcgc tatgtctcag ccaattacgc gtgaaaactt
     9181 tgatgaatgg atgatacctg tttacgctcc ggcacccttt ataccggtac gtggcgaagg
     9241 ttcgcgcttg tgggatcagc aggggaaaga gtatatcgac ttcgcgggtg gcattgcggt
     9301 gaacgcgctg ggccatgcgc atccggaact gcgtgaagcg ctgaacgaac aggcgagtaa
     9361 gttctggcat accggcaacg gttacaccaa cgagccggta ctgcgactgg cgaaaaaatt
     9421 gatcgacgcc acgtttgccg atcgcgtctt cttttgtaac tccggtgcgg aagccaacga
     9481 agcggcgcta aaactggcgc gtaaattcgc tcacgaccgc tacggcagcc ataagagcgg
     9541 tatcgtggcg tttaaaaatg cgtttcatgg tcgcacgctg tttactgtca gtgcgggtgg
     9601 gcagccagcc tattcacagg attttgcgcc actgccacca gatatccgtc atgctgcata
     9661 taacgatatt aactctgcca gcgcgctgat tgacgacgcc acctgtgcgg tgattgtcga
     9721 acccatccag ggggaaggcg gtgtggtgcc agccagcaac gcgtttttac aaggtctgcg
     9781 tgaattgtgt gaccgccaca atgcgctgtt gatttttgat gaagtacaaa ccggcgtcgg
     9841 gcgcaccggg gaactgtatg cctatatgca ctacggcgtg acacctgatc tgttaactac
     9901 cgccaaagcg ctgggcggcg gtttcccggt cggtgcgttg ttaacaaccg aagagtgcgc
     9961 cagcgtgatg accgttggca ctcatggcac cacctatggc ggcaatccgc tggcctcggc
    10021 ggtggcgggc aaagtgctgg agctcatcaa cacaccagag atgcttaatg gcgttaaaca
    10081 gcgtcacgac tggtttgttg agcgtcttaa taccattaat caccgctacg gtttgttcag
    10141 tgaagttcgc ggcttaggtt tgttgattgg ctgtgtactg aatgccgatt acgccggaca
    10201 agcgaaacag atctctcagg aggcggcgaa agcaggcgtt atggtactga ttgcgggtgg
    10261 caacgtggtg cgttttgcgc ctgcgctcaa tgtcagcgaa gaagaggtga cgaccggact
    10321 ggatcgcttt gcagctgctt gcgaacactt tgttagccga ggttcatcat gatggtcatc
    10381 cgtcccgttg agcgctcaga tgtctcggcg ctgatgcagc ttgccagcaa aacgggcggc
    10441 ggcctgacgt cgcttcccgc caatgaagcc acgctttccg cgcgtatcga aagggcaatc
    10501 aaaacctggc aaggcgaact gcccaaaagc gagcagggct atgtgttcgt gctggaagat
    10561 agcgagacag gcaccgtggc ggggatttgc gccattgagg tggcggttgg gctgaacgat
    10621 ccctggtaca actatcgggt cggcacgttg gttcacgcct caaaagagct gaatgtctat
    10681 aacgcattgc cgacgctgtt tcttagtaac gatcacaccg gcagcagcga gctgtgcacg
    10741 ctgtttctcg acccggactg gcgcaaagag ggcaacggct atttgctgtc gaaatcgcgc
    10801 tttatgttta tggcggcttt tcgcgacaag tttaatgaca aagtggttgc tgaaatgcgc
    10861 ggggtgattg acgaacacgg ctattcaccg ttctggcaaa gcctcggtaa acgcttcttt
    10921 tcgatggatt ttagccgcgt cgattttctc tgcggcaccg ggcaaaaggc atttattgca
    10981 gaactgatgc cgaaacatcc gatctatacc cactttttat cccaggaagc ccaggacgtc
    11041 atcggtcagg tacatccgca aaccgcgcct gcccgcgcgg tgctggagaa agaaggtttt
    11101 cgttaccgca actatatcga catcttcgac ggcgggccaa cgcttgagtg tgacatcgac
    11161 cgcgtgcgcg ccatccgtaa aagtcggctg gtggaagtgg cagaagggca gcctgcgcag
    11221 ggcgattttc cggcctgcct ggtcgccaat gaaaattatc accatttccg cgtggtgctg
    11281 gcgcgtaccg atccggcaac cgagcgtttg attttaaccg ccgcacaact ggatgccctc
    11341 aaatgccacg caggggatcg cgttcgtctg gtgcgcctgt gcgcagagga gaaaacagca
    11401 tgactttatg gattaacggt gactggataa cgggccaggg cgcatcgcgt gtgaagcgta
    11461 atccggtatc gggcgaggtg ttatggcaag gcaatgatgc cgatgccgct caggtcgaac
    11521 aggcttgtcg ggcagcccgt gcggcgtttc cgcgctgggc gcggctctca ttggctgaac
    11581 gtcaggtcgt tgtcgaacgc tttgccggat tgctggaaag caataaagcc gaattaaccg
    11641 cgattattgc cagagaaacg ggtaagccgc gctgggaagc ggcaaccgaa gtgacggcga
    11701 tgatcaataa aatcgcgata tcaattaagg cgtatcacgt tcgtaccggc gagcagcgta
    11761 gtgaaatgcc ggacggtgcg gcgagcctgc gacatcgccc gcacggcgtg ctggcggtgt
    11821 ttgggccgta taatttccct ggtcatttgc cgaacggaca tatcgttccg gcattgctgg
    11881 caggtaacac cattatcttt aaacccagcg aactgacacc gtggagtggc gaagcggtaa
    11941 tgcgtttatg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg cagggcgggc
    12001 gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg tttaccggta
    12061 gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag aaaattctcg
    12121 cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat atcgactcgg
    12181 ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc acctgcgccc
    12241 gccgtttatt gctcaaaagc ggagcacagg gcgatgcgtt tcttgctcgt ctggttgccg
    12301 tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt attggcgggc
    12361 tgatttctga acaggccgca cagcaggtgg ttactgccta gcagcaactg gaagcgatgg
    12421 gcggacgaac cctgcttgcg ccgcgcttat tacaatcaga gacatcgttg ctgacgccgg
    12481 ggatcattga aatgacaggc gttgctggcg taccagatga agaggtgttc ggaccgttat
    12541 tgcgcgtctg gcgttatgat tctttcgagg aagcgattct aatggcgaat aacactcgct
    12601 tcggactctc ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa ctgttgctgg
    12661 aggcgcgggt ggggattgtt aactggaaca aaccgcttac tggtgctgcc agtaccgcgc
    12721 cattcggcgg cattggtgca tccggtaacc atcgccccag cgcctggtat gccgcagatt
    12781 actgcgcatg gcctatggcg agcctggagt cggactcgtt aactttgccc gcaacgctta
    12841 accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag ttaatttcga
    12901 cgggctggta gggctgacgc atcattacgc tggcctgtcg tttggtaatg aagcctctac
    12961 ccgtcaccgt tttcaggtgt ctaacccgcg actggcggcg aaacagggct tactgaaaat
    13021 gaaaaccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg agcgtccgtt
    13081 tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac tggaaaaagt
    13141 tgcacgccag gcaccgcact ggctttccag cgtcagctcc gcttcgccaa tgtgggtagc
    13201 caatgcggca acgatcgcgc catctgccga tacgctggat ggtaaagtgc atctcaccgt
    13261 tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg aatcgctgtt
    13321 aaaagctatt tttaacgacg aagagaaatt tagcgtccat tcggcgttgc cgcaggtagc
    13381 gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt acggtgagcc
    13441 gggtatgcaa ctttttgtct acgggcgaga agaaggcaat gatacccggc cttcccgtta
    13501 tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc aggtgaatcc
    13561 ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagggtg tttttcataa
    13621 tgacgtgatt gccgtaagta accgccaggt gctgttttgc caccaacagg cgttcgctcg
    13681 ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg cgatagaagt
    13741 tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta acagccaact
    13801 gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc gggaacacgc
    13861 cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta gcgaactaaa
    13921 agtctttgat ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc tgcggttgcg
    13981 ggtagtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga tgaacgatac
    14041 gctgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgcc ttactgctgc
    14101 cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg tattgagcca
    14161 attactgaat ctcggttcgg tttatccgtt ccagcgcgag ggagggggca atggataatt
    14221 ttcttgctct gaccttaacg ggtaaaaaac cggttatcac cgagcgagaa atcaacggcg
    14281 ttcgctggcg ctggctgggc gatggtgtgc tggaactgac gccattaacg ccaccgcaag
    14341 gcgcgctggt gatttctgcg gggatccacg gtaatgagac ggcacctgtg gaaatgctgg
    14401 acgcgttgct tggcgcgata tctcacggcg agatcccgtt acgttggcgg ttgctggtga
    14461 tcctcgggga tcctcctgcg ctgaagcaag ggaaacgtta ttgccatagc gatatgaacc
    14521 gaatgtttgg cggtcgttgg cagctatttg ctgaaagcgg agaaacctgt cgggcgcgcg
    14581 aactggaaca gtgcctggaa gatttttatg accagggcaa agaatctgtg cgttggcacc
    14641 ttgatctaca taccgcaatt cgtggctcct tgcatcctca gttcggtgta ttaccgcaac
    14701 gcgacattcc ctgggacgag aaatttctga cgtggctggg tgcggcgggg ctggaggcgc
    14761 tggtgttcca tcaggagcct ggtggtacgt ttacccattt cagcgccaga cattttggcg
    14821 cgctggcctg tacgctggaa cttggcaaag cgttgccctt tgggcaaaac gatcttcgcc
    14881 agtttgcagt aactgccagc gcaattgctg cgctgctatc tggtgagagt gtcggtatcg
    14941 tgagaacacc gccgctccgt tatcgggtgg tttcgcaaat tactcgccac tcgccgtcct
    15001 tcgaaatgca tatggcaagt gacacgctga attttatgcc gtttgagaaa ggaacattgc
    15061 tggcgcagga cggagaggaa cgttttaccg taacccatga tgtagagtat gtgttattcc
    15121 ctaatccgtt ggtagcgttg ggattacgcg cgggattaat gctcgaaaaa ataagctaat
    15181 acttacccgc agaaatcatt ctgcg
//
LOCUS       DAZOZD010000092        15204 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOZD010000092 DAZOZD010000000
VERSION     DAZOZD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.092"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUPB_002290"
     CDS             <1..1015
                     /locus_tag="ACHUPB_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0759087.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUPB_002291"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUPB_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR0759088.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUPB_002292"
     CDS             1797..1904
                     /locus_tag="ACHUPB_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0759089.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUPB_002293"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUPB_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0759090.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUPB_002294"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUPB_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0759091.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHUPB_002295"
                     /pseudo
     CDS             complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHUPB_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHUPB_002296"
     CDS             complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHUPB_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0759092.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHUPB_002297"
     CDS             complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHUPB_002297"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0759093.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8365..10080
                     /gene="dld"
                     /locus_tag="ACHUPB_002298"
     CDS             8365..10080
                     /gene="dld"
                     /locus_tag="ACHUPB_002298"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0759094.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHUPB_002299"
     CDS             complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHUPB_002299"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0759095.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11225..11812)
                     /locus_tag="ACHUPB_002300"
     CDS             complement(11225..11812)
                     /locus_tag="ACHUPB_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0759096.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11982..12560
                     /locus_tag="ACHUPB_002301"
     CDS             11982..12560
                     /locus_tag="ACHUPB_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0759097.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12690..13451)
                     /locus_tag="ACHUPB_002302"
     CDS             complement(12690..13451)
                     /locus_tag="ACHUPB_002302"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0759098.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHUPB_002303"
     CDS             complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHUPB_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR0759099.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3710 a         4150 c         3808 g         3536 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgcagtg
     3781 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
     3841 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3901 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc
     3961 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     4021 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     4081 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     4141 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     4201 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     4261 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     4321 catcactaca ggccagcaat gccagcgccg cggccagcca gaaaccactg ccgaggctgg
     4381 tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttcct gctccccaca
     4441 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg
     4501 gaataaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     4561 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtaactga
     4621 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     4681 tacgtagata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     4741 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
     4801 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
     4861 gcaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc
     4921 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
     4981 taacgccaga agtttgctgg gcagcggctt taatcgttac cgccgtgtcg ccgccagcca
     5041 gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg
     5101 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     5161 gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
     5221 cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag ccagattaac ttgttttgct
     5281 ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt
     5341 tttcatcttt aaagaagaaa gcgccattac cggtatattc cggatagata tccagttcac
     5401 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     5461 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     5521 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     5581 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     5641 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     5701 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     5761 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     5821 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5881 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5941 aggttcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     6001 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     6061 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     6121 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
     6181 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     6241 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     6301 cttgtcggca ttatacggac gaccggtatt cagatggctg tagtacaccg ggatctgccc
     6361 gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac
     6421 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     6481 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     6541 tttaccggtg gctttcagcg ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     6601 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccac
     6661 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     6721 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     6781 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6841 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg
     6901 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6961 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg
     7021 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     7081 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     7141 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     7201 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     7261 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     7321 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     7381 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     7441 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     7501 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     7561 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     7621 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     7681 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     7741 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     7801 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7861 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7921 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7981 gttatccgga ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     8041 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
     8101 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     8161 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     8221 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     8281 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     8341 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     8401 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     8461 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     8521 ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     8581 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     8641 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     8701 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     8761 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     8821 ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa
     8881 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8941 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     9001 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     9061 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     9121 tcttctggtt gcgccgggaa gctggcggtc tttgctgtac gtctagatac cttcgaagcg
     9181 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     9241 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatacaccgg
     9301 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     9361 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     9421 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     9481 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     9541 ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggctggt ggattatttc
     9601 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     9661 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     9721 gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
     9781 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9841 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9901 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9961 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
    10021 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    10081 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
    10141 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
    10201 agcatcactt taccagtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    10261 tatttgccaa acgcatccat cactaccagc gctaccggtt tattattgat aaccgtacgc
    10321 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
    10381 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    10441 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
    10501 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    10561 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    10621 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
    10681 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
    10741 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    10801 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10861 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10921 atcgcgctac cggaggcaat ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca
    10981 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    11041 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    11101 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    11161 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    11221 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    11281 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    11341 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    11401 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
    11461 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    11521 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    11581 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    11641 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    11701 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    11761 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    11821 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11881 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11941 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    12001 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    12061 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    12121 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    12181 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    12241 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    12301 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    12361 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    12421 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    12481 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    12541 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    12601 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    12661 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    12721 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    12781 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12841 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12901 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg
    12961 gttaacccgc cgagcgcatg tttagcggct gtgtaagcgc tggcatccgg cagcggcgta
    13021 tgttcatgta ccgacgtaat gttgatgata cgaccgccct gcccttgttt caccatctgg
    13081 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    13141 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    13201 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    13261 agtttaccga gctccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    13321 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    13381 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    13441 acctgtgcca tcaagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    13501 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatagaag catccagcca
    13561 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    13621 gacgcgggaa ccaacaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    13681 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    13741 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    13801 agacaagttg ctttcggctt ttgcgatatt gagattggcg ttaagacgac cactatcgaa
    13861 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13921 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13981 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    14041 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    14101 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    14161 ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc gggttttgct
    14221 ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct gatatagctg
    14281 gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa
    14341 cgccgcctgg gtttgccact cccagtacag gcgggctacg ctgccagcca gcaattggcg
    14401 ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc aggcgggcag taacctccgc
    14461 ccgattcttt ccccagatat cgagatgcca gcccgccgtt aagccaaaag taccgttggt
    14521 gtaccacggg ccggtcgtac ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc
    14581 ttctgccgac attttttgcc gctccatatc cgccgaaaag tcgatctgcg gaccatcctg
    14641 agtggcaact gccttcgcct gggcttcagc tagctgaatg cgctgttcag ccacctgcat
    14701 atccggtgcg ttctgtagtg cattgttaat taatgaagtg agctgattat cgtgatactc
    14761 cagccaccat tggctgtctg gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac
    14821 ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc
    14881 ggccagcatc agtaacagcg gaaaacaggc gatggctgga taaaaggaat cacgattcat
    14941 gggggaataa tcaggtaaga aacggtgcgc ggagattacc gtgtgttgcg atatattttt
    15001 tagtttcgcg tggcaataca tcagtagcaa taaaacgaca tacccagaaa aatatagact
    15061 aagtaaatga tatcttccga tttatcttaa tcgtttatgg ataacggcaa agggcttcgt
    15121 tttttcctat acttattcag cactcacaaa taaaggaacg ccaatgaaaa ttatactctg
    15181 ggctgtattg attattttcc tgat
//
LOCUS       DAZOZD010000093        15108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOZD010000093 DAZOZD010000000
VERSION     DAZOZD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.093"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /locus_tag="ACHUPB_002304"
     CDS             complement(<1..214)
                     /locus_tag="ACHUPB_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFR0759100.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSL"
     gene            431..1462
                     /gene="idnD"
                     /locus_tag="ACHUPB_002305"
     CDS             431..1462
                     /gene="idnD"
                     /locus_tag="ACHUPB_002305"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR0759101.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            1486..2250
                     /gene="idnO"
                     /locus_tag="ACHUPB_002306"
     CDS             1486..2250
                     /gene="idnO"
                     /locus_tag="ACHUPB_002306"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR0759102.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            2315..3634
                     /gene="idnT"
                     /locus_tag="ACHUPB_002307"
     CDS             2315..3634
                     /gene="idnT"
                     /locus_tag="ACHUPB_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR0759103.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            3701..4699
                     /gene="idnR"
                     /locus_tag="ACHUPB_002308"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             3701..4699
                     /gene="idnR"
                     /locus_tag="ACHUPB_002308"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR0759104.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            4777..6279
                     /locus_tag="ACHUPB_002309"
     CDS             4777..6279
                     /locus_tag="ACHUPB_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0759105.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(6398..7480)
                     /gene="lptG"
                     /locus_tag="ACHUPB_002310"
     CDS             complement(6398..7480)
                     /gene="lptG"
                     /locus_tag="ACHUPB_002310"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0759106.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7480..8580)
                     /gene="lptF"
                     /locus_tag="ACHUPB_002311"
     CDS             complement(7480..8580)
                     /gene="lptF"
                     /locus_tag="ACHUPB_002311"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0759107.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(8643..8693)
                     /gene="ytgA"
                     /locus_tag="ACHUPB_002312"
     CDS             complement(8643..8693)
                     /gene="ytgA"
                     /locus_tag="ACHUPB_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0759108.1"
                     /translation="MSIIILATISTTQENK"
     gene            8847..10358
                     /gene="pepA"
                     /locus_tag="ACHUPB_002313"
     CDS             8847..10358
                     /gene="pepA"
                     /locus_tag="ACHUPB_002313"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0759109.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10492..10935
                     /gene="holC"
                     /locus_tag="ACHUPB_002314"
     CDS             10492..10935
                     /gene="holC"
                     /locus_tag="ACHUPB_002314"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0759110.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            10935..13790
                     /gene="valS"
                     /locus_tag="ACHUPB_002315"
     CDS             10935..13790
                     /gene="valS"
                     /locus_tag="ACHUPB_002315"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0759111.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(13846..15042)
                     /locus_tag="ACHUPB_002316"
     CDS             complement(13846..15042)
                     /locus_tag="ACHUPB_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0759112.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         3923 a         3753 c         3864 g         3568 t
ORIGIN      
        1 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
       61 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
      121 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
      181 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
      241 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
      301 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
      361 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
      421 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
      481 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
      541 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
      601 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
      661 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
      721 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
      781 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
      841 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
      901 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
      961 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     1021 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     1081 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     1141 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     1201 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     1261 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     1321 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     1381 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     1441 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     1501 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     1561 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     1621 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     1681 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     1741 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     1801 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     1861 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     1921 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     1981 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     2041 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     2101 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     2161 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     2221 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     2281 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     2341 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     2401 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     2461 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     2521 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     2581 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     2641 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     2701 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     2761 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     2821 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     2881 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     2941 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     3001 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     3061 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     3121 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     3181 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     3241 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     3301 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     3361 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     3421 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     3481 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     3541 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     3601 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     3661 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     3721 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     3781 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     3841 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     3901 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     3961 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     4021 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     4081 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     4141 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     4201 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     4261 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     4321 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     4381 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     4441 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     4501 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     4561 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     4621 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     4681 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     4741 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     4801 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     4861 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     4921 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     4981 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     5041 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     5101 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     5161 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     5221 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     5281 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     5341 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     5401 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     5461 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     5521 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     5581 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     5641 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     5701 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     5761 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     5821 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     5881 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     5941 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     6001 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     6061 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     6121 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     6181 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     6241 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     6301 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     6361 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     6421 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     6481 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     6541 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     6601 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     6661 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     6721 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     6781 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     6841 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     6901 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     6961 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     7021 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     7081 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     7141 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     7201 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     7261 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     7321 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     7381 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     7441 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     7501 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     7561 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     7621 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
     7681 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
     7741 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
     7801 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
     7861 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
     7921 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
     7981 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
     8041 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
     8101 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
     8161 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
     8221 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
     8281 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
     8341 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
     8401 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
     8461 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
     8521 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
     8581 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
     8641 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
     8701 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
     8761 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
     8821 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
     8881 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
     8941 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
     9001 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
     9061 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
     9121 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
     9181 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
     9241 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
     9301 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
     9361 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
     9421 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
     9481 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
     9541 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
     9601 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
     9661 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
     9721 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
     9781 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
     9841 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
     9901 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
     9961 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    10021 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    10081 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    10141 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    10201 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    10261 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    10321 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    10381 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    10441 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    10501 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    10561 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    10621 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    10681 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    10741 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    10801 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    10861 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    10921 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    10981 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    11041 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    11101 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    11161 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    11221 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    11281 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    11341 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    11401 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    11461 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    11521 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    11581 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    11641 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    11701 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    11761 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    11821 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    11881 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    11941 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    12001 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    12061 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    12121 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    12181 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    12241 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    12301 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    12361 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    12421 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    12481 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    12541 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    12601 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    12661 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    12721 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    12781 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    12841 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    12901 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    12961 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    13021 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    13081 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    13141 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    13201 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    13261 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    13321 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    13381 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    13441 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    13501 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    13561 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    13621 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    13681 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    13741 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    13801 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    13861 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    13921 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    13981 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    14041 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    14101 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    14161 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    14221 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    14281 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    14341 aaatgcgaga atggcatatt ttatacaata tgtagcatct aatttcacct tgaaacgatg
    14401 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    14461 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    14521 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    14581 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    14641 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    14701 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    14761 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    14821 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    14881 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    14941 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    15001 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    15061 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZOZD010000094        14965 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOZD010000094 DAZOZD010000000
VERSION     DAZOZD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.094"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUPB_002317"
     CDS             <1..196
                     /locus_tag="ACHUPB_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759113.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUPB_002318"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHUPB_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0759114.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="ACHUPB_002319"
     CDS             complement(1608..1931)
                     /locus_tag="ACHUPB_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0759115.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUPB_002320"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHUPB_002320"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0759116.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUPB_002321"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHUPB_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0759117.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUPB_002322"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHUPB_002322"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0759118.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUPB_002323"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHUPB_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0759119.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="ACHUPB_002324"
     CDS             7531..8568
                     /locus_tag="ACHUPB_002324"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0759120.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUPB_002325"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHUPB_002325"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0759121.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUPB_002326"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHUPB_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0759122.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUPB_002327"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHUPB_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0759123.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="ACHUPB_002328"
     CDS             10739..11620
                     /locus_tag="ACHUPB_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0759124.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUPB_002329"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHUPB_002329"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0759125.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUPB_002330"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="ACHUPB_002330"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0759126.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="ACHUPB_002331"
     CDS             complement(14017..>14965)
                     /locus_tag="ACHUPB_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0759127.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAZOZD010000095        14750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOZD010000095 DAZOZD010000000
VERSION     DAZOZD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.095"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            347..1636
                     /gene="sdaC"
                     /locus_tag="ACHUPB_002332"
     CDS             347..1636
                     /gene="sdaC"
                     /locus_tag="ACHUPB_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0759128.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1694..3061
                     /gene="sdaB"
                     /locus_tag="ACHUPB_002333"
     CDS             1694..3061
                     /gene="sdaB"
                     /locus_tag="ACHUPB_002333"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0759129.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3173..3928
                     /gene="xni"
                     /locus_tag="ACHUPB_002334"
     CDS             3173..3928
                     /gene="xni"
                     /locus_tag="ACHUPB_002334"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0759130.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4033..5181)
                     /gene="fucO"
                     /locus_tag="ACHUPB_002335"
     CDS             complement(4033..5181)
                     /gene="fucO"
                     /locus_tag="ACHUPB_002335"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0759131.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5209..5856)
                     /gene="fucA"
                     /locus_tag="ACHUPB_002336"
     CDS             complement(5209..5856)
                     /gene="fucA"
                     /locus_tag="ACHUPB_002336"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0759132.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6403..7719
                     /gene="fucP"
                     /locus_tag="ACHUPB_002337"
     CDS             6403..7719
                     /gene="fucP"
                     /locus_tag="ACHUPB_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0759133.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            7752..9527
                     /gene="fucI"
                     /locus_tag="ACHUPB_002338"
                     /pseudo
     CDS             7752..9527
                     /gene="fucI"
                     /locus_tag="ACHUPB_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            9636..11054
                     /gene="fucK"
                     /locus_tag="ACHUPB_002339"
     CDS             9636..11054
                     /gene="fucK"
                     /locus_tag="ACHUPB_002339"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR0759134.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            11056..11478
                     /gene="fucU"
                     /locus_tag="ACHUPB_002340"
     CDS             11056..11478
                     /gene="fucU"
                     /locus_tag="ACHUPB_002340"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0759135.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11536..12267
                     /gene="fucR"
                     /locus_tag="ACHUPB_002341"
     CDS             11536..12267
                     /gene="fucR"
                     /locus_tag="ACHUPB_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0759136.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12311..13411)
                     /gene="rlmM"
                     /locus_tag="ACHUPB_002342"
     CDS             complement(12311..13411)
                     /gene="rlmM"
                     /locus_tag="ACHUPB_002342"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0759137.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13404..13799)
                     /locus_tag="ACHUPB_002343"
     CDS             complement(13404..13799)
                     /locus_tag="ACHUPB_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0759138.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13818..14735)
                     /gene="gcvA"
                     /locus_tag="ACHUPB_002344"
     CDS             complement(13818..14735)
                     /gene="gcvA"
                     /locus_tag="ACHUPB_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0759139.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         3624 a         3870 c         3609 g         3647 t
ORIGIN      
        1 catgattaac taaaagtatt gattttttca gttcaaccta catatattgc gcgccccgga
       61 agaagtcaga tgtcgtttaa ttggcaaata ttgcccttaa attctctttt acttttgatt
      121 tacagagtaa agcgttggga taatctatct tccaagtaga ttattgtatt tgggatcaag
      181 atcactgata gatacataac ttgtgtgtat ctttccgccc tcaaattatt acggcggtaa
      241 atgattaagc catcgccgat agacagattt catttttacg gtcaggcacc ttcccgagct
      301 gaactggcta aaagctgaat tatttgcatt cctccaggag aaatagatgg aaacgactca
      361 aaccagcacg attgcgtcga aagactctcg tagtgcctgg cgcaagacag acaccatgtg
      421 gatgctgggc ctttacggca cggcaatcgg cgcgggcgtg ctgttcctgc caatcaacgc
      481 cggtgttggc ggtatgatcc cgctgatcat catggctatc cttgcgttcc cgatgacatt
      541 ttttgctcac cgcggcctga ctcgcttcgt actgtctggt aaaaacccag gcgaagacat
      601 caccgaggtt gtagaagaac actttggtat tggcgcaggt aaactgatta ccctgctcta
      661 cttcttcgct atctacccga tcctgctggt ttatagcgtg gcaatcacca atacagttga
      721 aagcttcatg tctcaccagc tgggtatgac accaccgccg cgtgcgattc tgtcgctgat
      781 cctgatcgtg ggtatgatga ccatcgttcg cttcggtgag cagatgatcg ttaaagcaat
      841 gagtattctg gtattcccgt ttgttggcgt actgatgctg ctggctctgt acctgatccc
      901 gcagtggaac ggcgcagcac tggaaacgct gtctctggac actgcatctg caaccggaaa
      961 cggtctgtgg atgaccctgt ggctggcaat tccggttatg gtgttctctt tcaaccactc
     1021 tccgatcatc tcttctttcg ccgttgcgaa gcgtgaagag tacggcgata tggcagaaca
     1081 gaaatgctcg aagatcctgg cattcgcaca catcatgatg gtgctgaccg taatgttctt
     1141 cgtcttcagc tgtgtactga gcctgactcc ggcagacctg gctgcggcta aagagcagaa
     1201 catctcgatt ctgtcttacc tggctaacca ctttaacgca ccggttatcg cgtggatggc
     1261 tccgattatc gcgattatcg ctatcaccaa atccttcctc ggccactacc tgggcgcacg
     1321 tgaaggcttc aacggtatgg tgattaaatc tctgcgtggt aaaggtaagt ctatcgaaat
     1381 caacaagctg aaccgtatca ctgcgctgtt catgctggta acgacctgga ttgttgccac
     1441 cctgaacccg agcatcctgg gtatgattga aaccctgggc ggcccaatca tcgcgatgat
     1501 cctgttcctg atgccgatgt acgcaattca gaaagtaccg gcaatgcgta agtacagcgg
     1561 tcacatcagc aacgtattcg ttgtcgtgat gggtctgatt gcaatctccg caatcttcta
     1621 ctctctgttc agctaagtcc tttcgcgccg ctttcgggcg gcgcttcctc cgttttaaca
     1681 cgatgtattt cctatgatta gcgtattcga tattttcaaa atcggcattg gcccttccag
     1741 ttctcatacc gttggaccaa tgaaagcggg taaacaattt accgacgatc tgattgcccg
     1801 taatctgctt aaagacgtga cccgcgtggt ggttgacgtg tacggctcgc tctctctgac
     1861 cggtaaaggc caccacactg atatcgccat tattatgggc ctggcgggta acctgccgga
     1921 taccgtggat atcgattcca tccctggttt tattcaggat gtgaatactc atggtcgcct
     1981 gatgctggca aacggtcagc atgaagtgga gttcccggtt gatcagtgca tgaacttcca
     2041 tgcagacaac ctttctctgc atgaaaacgg tatgcgcatt accgcgctgg cgggcgataa
     2101 agtcgtttac agccagactt actactctat tggcggtggc tttatcgttg atgaagagca
     2161 ttttggccag cagaatagcg caccggttga agttccttat ccgtacagtt cagcagccga
     2221 tctgcaaaaa cattgtcagg aaaccgggct gtcactctct ggcctgatga tgaaaaatga
     2281 gctggcgctg cacagcaaag aagagctgga acagcacctg gcgaacgtat gggaagtcat
     2341 gcgtggcggt attgagcgcg gtatttccac cgaaggcgtg ttgcctggca aactgcgcgt
     2401 tccacgccgt gctgcggcac tacgccggat gctggtcagc caggataaaa ccaccactga
     2461 cccgatggcg gttgttgact ggatcaacat gtttgcactg gcagtgaacg aagagaacgc
     2521 tgctggcgga cgcgtggtga ctgcgccgac taacggtgcg tgcgggatta tcccggcagt
     2581 gctggcgtac tacgacaagt tcatccgcga agtgaacgct aactcactgg ctcgttacct
     2641 gctggtagcc agtgccattg gttctcttta taagatgaac gcgtcaattt ctggtgctga
     2701 agtcggctgc cagggtgaag ttggtgtggc gtgctcaatg gcggcggctg gtctggcaga
     2761 actgttaggt gcaagcccgg cgcaggtgtg catcgcggcg gaaatcgcca tggagcacaa
     2821 cctcggtctg acgtgtgacc cggtcgccgg acaggtccag gtgccatgca tcgagcgtaa
     2881 cgccattgcg gcagtaaaag cggtgaacgc ctcacgtatg gcgctacgcc gtaccagcga
     2941 gccgcgcgtc tgcctcgata aagttatcga aaccatgtac gaaacaggta aagatatgaa
     3001 cgccaagtac cgcgaaacct ctcgcggcgg cctggcaatg aagatcgttg cctgcgatta
     3061 atcgctctcc agaggcctcg ttttgcgagg cctcttcccg atttctcatc cagccgtagc
     3121 ctgttccggc atcgaatgtt acccttatcg cctgatcttt aagggggtta tcgtggctgt
     3181 tcatttgctt attgtcgatg cactgaatct tattcgtcgc attcatgccg ttcagggatc
     3241 gccctgtgtc gaaacctgcc agcatgcgct cgatcagctc attatgcaca gccagccaac
     3301 ccacgcggtc gccgtttttg atgatgaaaa ccgcagtagc ggctggcgtc atcagcgttt
     3361 accagattac aaagcgggtc gaccgccaat gccggaagag ttgcacgacg agatgcctgc
     3421 attacgcgcc gcctttgagc aacgcggcgt cccgtgctgg tcaaccagcg gcaacgaagc
     3481 cgatgactta gccgccacgc tggcggtcaa agtgacacag gccgggcatc aggcaacgat
     3541 tgtttcgaca gataaaggct actgtcagtt actttcaccg acattacgta ttcgtgatta
     3601 cttccagaaa cgttggctgg atgcgccatt tatcgataaa gaatttggcg ttcaaccgca
     3661 gcagttgccc gattactggg gacttgcggg gatcagcagt tcaaaggtac cgggtgttgc
     3721 gggaatcgga ccaaaaagcg ccacgcagct gctggtcgag tttcagagtc tggaagggat
     3781 atatgagaat ctggatgcgg ttgccgaaaa gtggcgcaaa aaattagaaa cccataaaga
     3841 gatggcgttt ctgtgccgcg atattgcccg cttacaaacc gatttgcata tcgacggcaa
     3901 tttacagcaa ttgcggttgg tacggtaacg gcgagccgga tgcaaacgat tatcaacaca
     3961 ttgaatttaa tgcacttata taccggataa gacgcggcta gcgtcgtatc cggcattatc
     4021 acatcagcgc atttaccagg cggtatggta aagctctaca atatcctcaa gcgttgcttc
     4081 acgcgggttg ccaccggtac aaacatcatc cagtgccgcc tgcgccagtg ccggaatgtc
     4141 ttccttgcgt acaccaacat cacgcaaatg tggcggaata ccgacatcac ggttgagagc
     4201 aaacaccgct tcaacagcgg cattacgcgc ctcttccagg ctcatacctt ccactttcac
     4261 gcccataacg cgcgcgatat cgcggtactt ctcaccggta aagtcagcgt tatagtgcat
     4321 gacatgcggt aacaggatgg cgttcgcaac accgtgtgga gtgttgtaaa acgcgcccag
     4381 tggatgcgcc ataccatgca ccaaccctaa cccaacattc gagaagccca tacccgcaac
     4441 atactgcccg agcgccattt cttctccggc atccttatca ccagcaaccg atcctcgcag
     4501 cgccccagca atgatttcaa tcgctttaat gtgcagtgca tcggttagcg cccacgcgcc
     4561 acgggtaata tacccctcaa tagcatgagt gagcgcatcg acacccgtcg cagctttcag
     4621 cgctggaggc ataccatcca tcatgtcagc gtcaataaac gccacctgcg ggatatcatg
     4681 cggatcaacg caaacaaact tgcgccgttt ctcttcgtca gtgatcacgt agttaatggt
     4741 cacttctgcc gcagtacctg ctgtggtagg aattgccaga atcggtacac tgggtttatt
     4801 ggtcggggaa agcccttcca ggctacgcac atcggcaaac tccggattgt tgctgataat
     4861 gccaatcgct ttacaagtat cctgtggaga accaccacca atagcgatca ggtaatccgc
     4921 gccgctattc tggaatacac cgagcccttc tttgacgaca gtaattgttg ggttgggcac
     4981 tacgccgtcg taaatcgccc atgccagccc tgcagcatcc atcttatcgg tcactttcgc
     5041 caccacgccg cattgcacca gcgttttatc ggtgacgatc agcgccttct gataaccacg
     5101 gcgtttcacc tcatcggtta aagccccaac agcaccccga ccaaaccatg ccgtttcgtt
     5161 cagaatcatt ctgttagcca tcatccttct ccttgttgct ttacgaaatt actcttcaat
     5221 ccgtaaccca taggttttga atttctccag cactacggca atctcttcat cgctcagcac
     5281 tggcaccggg tccgtaatcg ccagggtcgc cacgtaaagt tgcgccagca cttcaacttc
     5341 atgcgccagc cataacgctt tttccagatt cacctcacaa gcgataagcc catgatgttg
     5401 taacaaagtt gccttacgat ttttgagagc cagcgcaaca tgttcagaaa gttcgcgtgt
     5461 tccaaaggtc gcataaggcg cgcaaggaat agaattacca cctgccgccg caatcatgta
     5521 gtgaatagcg gggatcggtc ggttaagaat ggaaactgct gtgcaatgaa cggcatgatt
     5581 gtgaacaacc gcgttggcat ccggtctgct ttgataggcc gccatatgga aacgccattc
     5641 gcttgagggg agctttcctt cctcatgttt accgttgcca tcaataaaga caatatgcga
     5701 ctccgtcagt ttttcatatg gaatgcctgt aggcgtaatc agcatcccat cctgataacg
     5761 tacactgacg ttccccgctg tcccctggtt cagtcccagg cgggtcattt ccaggcaagt
     5821 gtcaataatc tgacgagcaa gtttatttcg ttccattagc tacctctctc tgattcaaaa
     5881 cagggcaata atgttgttcc tttcacacta ttgaattagc cgtttaatta cccaccatct
     5941 tcttcctgat taacaagaaa gaaattcaca agcttatatt ttgtgaccta gctcaactaa
     6001 tcacagtaaa taactgcaag ttctcttttt ataaccccat taaaaatgac cgctcttaaa
     6061 aatatttatc aaaacggtca tttttctatt cacccaagcc aggaatgccc gttttcggca
     6121 taaacaatta atacagtcat ctggtttgtg ttttttatga tttattttct gaaacgggca
     6181 tgaaatttcg attattaaag tgatggtagt cacataaagt caccttccag ctaataagtg
     6241 tgaccgccgt catattacag agcgtttttt atttgaaaat gaatccatga gttcatttca
     6301 gacaggcaaa tattcactga tatgaagccc gaactcgctg gttttgcact tttgaaaaca
     6361 taaccgatta cgtgcttaag cttctgaacc taagaggatg ctatgggaaa cacatcaata
     6421 caaacgcaga gttaccgtgc ggtagataaa gatgcagggc aaagcagaag ttacattatt
     6481 ccattcgcgc tgctgtgctc actgtttttt ctttgggcgg tagccaataa ccttaacgac
     6541 attttattac ctcaattcca gcaggctttt acgctgacaa atttccaggc tggcctgatc
     6601 caatcggcct tttactttgg ttatttcatt atcccaattc ctgctgggat attgatgaaa
     6661 aaactcagtt ataaagcagg aattattact gggttatttt tatatgccct gggcgctgca
     6721 ttattctggc ccgctgcaga aataatgaac tacaccttat ttttagttgg cctatttatt
     6781 attgcagccg gattaggttg tctggaaact gccgcaaacc cttttgttac ggtattaggg
     6841 ccggaaagca gtggtcactt ccgcttaaat cttgcgcaaa catttaactc gtttggcgca
     6901 attatcgcgg ttgtctttgg gcaaagactt attttgtcta acgtgccaca tcaatcgcaa
     6961 gacgttctcg ataaaatgtc tccagagcaa ttgagtgcgt ataaacacag cctggtatta
     7021 tcggtacaga caccttatat gatcatcgtg gctatcgtgt tactggtcgc cctgctgatc
     7081 atgctgacga aattcccggc attgcagagt gataatcaca gtgacgccaa acaaggatcg
     7141 ttctccgcat cgctttctcg cctagcgcgt attcgccact ggcgctgggc agtattagcg
     7201 caattctgct acgttggcgc acaaacggcc tgctggagct atttgattcg ctacgctgta
     7261 gaagaaattc caggtatgac tgcaggcttt gccgctaact atttaaccgg aaccatggtg
     7321 tgcttcttta ttggtcgttt caccggtacc tggctcatca gtcgcttcgc accacacaaa
     7381 gtcctggccg cctacgcatt aatcgctatg gcactgtgcc tgatctcagc cttcgctggc
     7441 ggtcatgtgg gcttaatagc cctgacttta tgcagcgcct ttatgtcgat tcagtaccca
     7501 acaatcttct cgctgggcat taagaatctc ggccaggaca ccaaatacgg ttcgtccttc
     7561 atcgttatga ccattattgg cggcggtatt gtcactccgg tcatgggttt tgtcagtgac
     7621 gcggcgggca acatccccac tgctgaactg atccccacac tctgcttcgc ggtcatcttt
     7681 atctttgccc gtttccgctc tcaaacggca actaactgaa catattttcc gaataaagtg
     7741 aggaatctgt aatgaaaaaa atcagcttac cgaaaattgg tatccgcccg gttattgacg
     7801 gtcgtcgcat gggtgttcgt gagtcgcttg aagaacaaac aatgaatatg gcgaaagcta
     7861 cggccgcact gctgaccgag aaactgcgcc atgcctgcgg agctgccgtc gagtgtgtca
     7921 tttccgatac ctgtatcgcg ggtatggctg aagccgctgc ttgcgaagaa aaattcagca
     7981 gctagaatgt aggcctcacc attacggtaa cgccttgctg gtgctatggc agtgaaacca
     8041 tcgacatgga tccaacccgc ccgaaagcca tttggggctt taacggcact gaacgtcccg
     8101 gcgctgttta cctggcagcg gctctggcag ctcacagcca gaaaggcatc ccggcattct
     8161 ccatttacgg tcatgacgtt caggatgccg atgacacatc gattcctgcc gatgttgaag
     8221 aaaaactgct gcgctttgcc cgcgccggtt tggccgtcgc cagcatgaaa ggtaaaagct
     8281 atctgtcgct gggcggcgtt tcgatgggta tcgccggttc tattgttgat cacaacttct
     8341 ttgaatcctg gctgggaatg aaagtccagg cggtggatat gaccgaactg cgtcgccgta
     8401 tcgatcagaa gatttacgac gaagccgaac tggaaatggc actgacctgg gctgataaaa
     8461 acttccgcta tggcgaagat gaaaataaca aacagtatca acgcaatgcc gagcaaagcc
     8521 gcgcagttct gcgcgaaagt ttactgatgg cgatgtgtat ccgcgacatg atgcaaggca
     8581 acagcaaact ggccgatatt ggtcgcgtgg aagaatcact tggctacaac gccatcgctg
     8641 cgggcttcca ggggcaacgt cactggaccg atcaatatcc caatggtgac accgccgaag
     8701 cgatcctcaa cagttcattt gactggaatg gcgtgcgcga accctttgtc gtggcgaccg
     8761 aaaacgacag tcttaacggc gtggcaatgc taatgggtca ccagctcacc ggcaccgctc
     8821 aggtatttgc cgatgtgcgt acctactggt caccagaagc aattgagcgt gtaacggggc
     8881 ataaactcga tggactggca gaacacggca tcatccattt gatcaactca ggttctgctg
     8941 cgctggacgg ttcctgtaaa caacgcgaca gcgaaggtaa cccgacgatg aagccacact
     9001 gggaaatctc tcagcaagag gctgacgctt gcctcgccgc taccgaatgg tgcccggcga
     9061 tccacgaata cttccgtggc ggcggttact cttcccgctt ccttaccgaa ggcggcgtcc
     9121 cgttcaccat gactcgtgtc aacatcatca aaggcctggg atcggtactg caaatcgcgg
     9181 aaggctggag cgtggaattg ccgaaggatg tgcatgacat cctcaacaaa cgcaccaact
     9241 caacctggcc aaccacctgg tttgcaccgc gcctcaccgg taaagggccg tttacggatg
     9301 tgtactcggt aatggcgaac tggggcgcta accatggggt tctgaccatc ggccacgttg
     9361 gcgcagactt tatcactctc gcctccatgc tgcgtatccc ggtatgtatg cacaacgttg
     9421 aagagaccaa agtgtatcgt ccttctgcct gggctgcgca cggcatggat attgaaggcc
     9481 aggattaccg cgcttgccag aactacggtc cgttgtacaa gcgttaatac attactccct
     9541 gatgtgatgc ccggtcgctc cggctaccgt gcctgaacaa gcaagagtgg ttagccggat
     9601 aagcaatgtt atccggctat attgcaggag cgattatgaa acaagaagtt atcctggtac
     9661 tcgactgtgg cgcgaccaat gtcagggcca tcgcggttaa tcggcaggga aaaattgttg
     9721 cccgcgcctc aacgcctaat gccagcgata tcgcgatgga aaacaacacc tggcaccagt
     9781 ggtctttaga cgccattttg caacgctttg ctgattgctg tcggcaaatc aatagtgaac
     9841 tgactgaatg ccacatccgc ggtatcgccg tcaccacctt tggtgtggat ggcgctctgg
     9901 tagataagca aggcaatctg ctctatccga tcattagctg gaaatgtccg cgaacagcag
     9961 cggttatgga caatattgaa cagttgatct ccgcacagcg gttgcaggct atttctggcg
    10021 tcggagcctt tagtttcaat acgttatata agttggtgtg gttgaaagaa aatcatccac
    10081 aactgctgga acgcgcgcac gcctggctct ttatttcgtc gctgattaac caccgtttaa
    10141 ccggcgaatt cactactgat atcacgatgg ccggaaccag ccagatgctg gatatccagc
    10201 aacgcgattt cagtccgcaa attttacaag ccaccggtat tccacgccga ctcttccctc
    10261 gtctggtgga agcgggtgaa cagattggta cgctacagaa cagcgccgca gcaatgctcg
    10321 gcttacccgt tggcataccg gtgatttccg caggtcacga tacccagttc gccctttttg
    10381 gcgctggtgc cgaccaaaat gaacccgtgc tctcttccgg tacatgggaa attttaatgg
    10441 tccgtagcgc ccaggttgat acttcgctgt taagtcagta cgccgattct acctgcgaac
    10501 tggatagcca ggcagggttg tataacccag gtatgcaatg gctggcatcc ggcgtgctgg
    10561 aatgggtgag aaaactgttc tggacggctg aaacaccctg gcaaatgttg attgaagaag
    10621 ctcgtctgat cgcacctggc gcggatggtg tgaaaatgca gtgtgattta ttgtcgtgtc
    10681 agaacgctgg ctggcaagga gtgacgctta ataccacgcg ggggcatttc tatcgcgcgg
    10741 cgctggaagg gttaactgcg caattacagc gcaatctaca gatgctggaa aaaatcgggc
    10801 actttaaagc ctctgaatta ttgttagtcg gtggaggaag tcgcaacaca ttgtggaatc
    10861 agattaaagc caatatgctt gatattccgg taaaagttct cgacgatgcc gaaacgaccg
    10921 tcgcaggagc tgcgctgttc ggttggtatg gcgtagggga atttaacagc ccggaagaag
    10981 cccgtgcaca gattcattat cagtaccgtt atttctaccc gcaaactgaa cctgaattta
    11041 tagaggaagt gtgaaatgct gaaaacaatt tcgccgttaa tttctcccga actattgaaa
    11101 gtgctggcag agatgggaca tggagatgaa attatttttt ccgatgctca ctttcccgcc
    11161 cattcgatgg gaccgcaggt gatccgcgct gatggcctgt tggtgagcga cttgctccaa
    11221 gcgattatcc cattatttga gctggacagt tatgccccgc cgctggtgat gatggcagcg
    11281 gtagaaggtg acactctcga tcctgaagta gaacgacgtt accgtaatgc gctttcacta
    11341 caagccccgt gtcctgacat catccgcatc aatcgttttg cgttttatga acgggcgcaa
    11401 aaagcctttg cgatcgttat cacaggcgaa cgagcgaagt acgggaatat tcttttaaaa
    11461 aaaggggtaa caccgtaatc tcatatcggt acgcccgcat gacgcgggcg gttatcgaat
    11521 gatggggtga aaaatatgaa agcggcacgc cagcaagcga tagtcgacct gctgctgaac
    11581 cataccagcc tgaccacgga agctctctct gaacagctaa aggtcagtaa agaaaccatt
    11641 cgtcgcgatc tcaatgaatt acagacgcag ggtaaaattc tgcgcaatca tggacgcgct
    11701 aaatatatcc accgtcaaaa tcaagacagt ggcgatccct ttcacatcag gctgaaaagc
    11761 cattatgcgc ataaagcaga tatcgcgcgc gaggcgctcg cgtggattga agaagggatg
    11821 gtgatagcct tagacgccag ttcaacttgc tggtatctgg cacgccagtt gcctgacatc
    11881 aacattcagg tcttcaccaa tagccatccg atttgccatg aactcggtaa acgcgaacgc
    11941 attcaactga tcagttccgg cggcacactt gagcgcaaat atggctgtta cgtcaatccc
    12001 tcgctgattt cccaacttaa atcgctggaa atcgatctgt ttattttttc ttgtgaaggg
    12061 atcgatagca gcggcgcact gtgggactcc aatgcgatca acgctgatta caaatcgatg
    12121 ctattaaaac gtgccgcgca atcgttgtta ttgattgata aaagtaaatt taatcgttca
    12181 ggggaagccc gcatcgggca tctggatgag gtaacgcaca ttatttctga tgagcgccag
    12241 gttgcaactt ctttggtaac agcctgacgg aaagggtagc aggccgaaga cgacgccccg
    12301 gccttgcctg ttatcgctcg tcgcgacgac cacccaccgc agcccagatg cggcggacgt
    12361 gcaccgtcac ttcttcgcga tcgtgataca actgccgcgc ctgaatctga gcatttatgc
    12421 catgttcatc aagctgtgcc tgaatatacg ccagattgtg tgatacttct tcgtagcgtt
    12481 ttttcatcgg cagtttgaga ttgaaaatgg tttcacggca ccagccatta accagccact
    12541 gcgccatcaa tgccgcaact ttagccggtt tttcaaccat atcgcatacc atccaggaga
    12601 tattgctgcg tgtcggacgg aatttgaaac cgtcttcccg cagccacgtc acctgcccgg
    12661 tatccatcag actttgcgcc atcgggccgt tgtcgacgga ataaacccac atgttgcgct
    12721 tcaccagttg gtaggtccag ccgccagggc aagcgcctaa atccaccgcc cacatcccgt
    12781 tcgccaggcg ttcatcccac tcatccgcag gaataaacac atgaaatgcc tcttccagtt
    12841 tgagcgtgga acgactcggc gcctctgccg gaaatttcag gcgcggaatg cccatataga
    12901 acggcgaatt attgttgctg tatgagtaac cggtatagca gcagcctggt gcaataaaga
    12961 atacatgcac aaccggacgc ttcggcgttt catagttcgc cagcaccccg gcatcgcgca
    13021 aggcagcgcg tagcggcacg gtgaatttac ggcagaattt cagcaactct ttgctttcgt
    13081 tggtatcggc aacttcaaca cgcagttcac cgcccttctc tactacgccc tgcaacatgt
    13141 cgacaatggg ggtaatacga tcttctggcg gcaaatgctg caagagttcc cccaccacaa
    13201 accactggcg ggcaaaaatt aatgaactga acggcagctc acggattaac ttatcgccat
    13261 catcaggttg ataacattca taaatgacat aacccgcatt ctctttcacg cgggcaaaac
    13321 cgaaaatttc ccgctggccg gctttatcgg taatttctgc ggcgcactct ttttcaaagc
    13381 ccggacggca cagcaataca accttattca tgacttacgc ccttgcgctt taaacggata
    13441 gcaccaacta acattaacgc ccagcccgcg aggaagctca cgccgccaac cggtgtgaca
    13501 aatgcccaca aacgcagatg ggacagcgcc aggcaataaa ggctgccgct gaacaacacc
    13561 gtgcctaacg cgaggaaaac gctactccag taaaaccaga tactgatgcg acgctgcatt
    13621 gccaccgcca gacctaagat cgccagcgta tgaaacgcct ggtattcgag gccggtctgg
    13681 atccagccca tctcaacggc ccccatggtt ttacttaaca catgcgcgcc aaaagcgccc
    13741 agagccacaa aaatgaagcc gctaatggcg gcgaaaatca gcataaaacg gctggtcatg
    13801 gtcgtaccct acgtaaatta ttgttcataa cgaaagcgga atttttcttg ttcagcagcg
    13861 gctttcgcca ggatccattg gcgaaaggcg gctattttac ccagttctgc ctggctgtca
    13921 tgacaaacca gataaaaagc gtttttactg accagaacat cattaaacgg gcaaacaaga
    13981 cgtccggcct cgatttcaga ttgcgccatc acgttatttg ccagcgccac tccctgcccg
    14041 tgaatagccg cttgcagcac catggcgcta tgactaaaaa ttggcccttg ctgaacgttg
    14101 atatgattta accccaactg tcgggtatat gtctgccagt cacggcgtga cgcatcatgt
    14161 aataacgtat gtttagccag atcttccggg gtcttcaagg gtttttcgcc tgtcagcagt
    14221 agcggcgaac acaccggcaa taaatattcg gcgtacagtt tttccacccg tagccccggc
    14281 cagttgcccc gaccataaaa tatcgccaca tcaacatcat ccgccagctt atcttcctga
    14341 cgatcaaccg cctggattcg aacgtcaatt cccggataag ctgaattaaa gctggaaagt
    14401 cgcggaacca accaatgaat ggcgaaactg gggagtaaac tgaccgtcaa cgcccccttg
    14461 gcgctacggg cctggagttt acgcgtcgct tcggttaatt gcgaaaatat ctctttgata
    14521 tcgaggaaat agctttgccc ttcctcggtc agcaggagtg aacgattacg gcggcggaac
    14581 agttttagcc ccaaaaaatc ctcaagagac ttgatttgat gacttactgc ggcctgggtc
    14641 acgaaaagct cttctgctgc gcgagtgaaa cttaaatggc gtgctgcggc atcaaaaact
    14701 cgtaaggcat ttagcggtgg taatcgttta gacatggctt ttaaactttg
//
LOCUS       DAZOZD010000096        14741 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOZD010000096 DAZOZD010000000
VERSION     DAZOZD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.096"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(181..333)
                     /gene="hokE"
                     /locus_tag="ACHUPB_002345"
     CDS             complement(181..333)
                     /gene="hokE"
                     /locus_tag="ACHUPB_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0759140.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            302..570
                     /locus_tag="ACHUPB_002346"
                     /pseudo
     CDS             302..570
                     /locus_tag="ACHUPB_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            773..1891
                     /locus_tag="ACHUPB_002347"
     CDS             773..1891
                     /locus_tag="ACHUPB_002347"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0759141.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1957..2205
                     /locus_tag="ACHUPB_002348"
                     /pseudo
     CDS             1957..2205
                     /locus_tag="ACHUPB_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            2270..2638
                     /locus_tag="ACHUPB_002349"
     CDS             2270..2638
                     /locus_tag="ACHUPB_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0759142.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2732..3385
                     /gene="nfsB"
                     /locus_tag="ACHUPB_002350"
     CDS             2732..3385
                     /gene="nfsB"
                     /locus_tag="ACHUPB_002350"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0759143.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3493..4740
                     /gene="ybdG"
                     /locus_tag="ACHUPB_002351"
     CDS             3493..4740
                     /gene="ybdG"
                     /locus_tag="ACHUPB_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0759144.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4808..6184)
                     /gene="pheP"
                     /locus_tag="ACHUPB_002352"
     CDS             complement(4808..6184)
                     /gene="pheP"
                     /locus_tag="ACHUPB_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0759145.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6286..9429)
                     /gene="cusA"
                     /locus_tag="ACHUPB_002353"
     CDS             complement(6286..9429)
                     /gene="cusA"
                     /locus_tag="ACHUPB_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0759146.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9441..10664)
                     /gene="cusB"
                     /locus_tag="ACHUPB_002354"
     CDS             complement(9441..10664)
                     /gene="cusB"
                     /locus_tag="ACHUPB_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR0759147.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10680..11012)
                     /gene="cusF"
                     /locus_tag="ACHUPB_002355"
     CDS             complement(10680..11012)
                     /gene="cusF"
                     /locus_tag="ACHUPB_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0759148.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(11036..12418)
                     /gene="cusC"
                     /locus_tag="ACHUPB_002356"
     CDS             complement(11036..12418)
                     /gene="cusC"
                     /locus_tag="ACHUPB_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0759149.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12575..13258
                     /gene="cusR"
                     /locus_tag="ACHUPB_002357"
     CDS             12575..13258
                     /gene="cusR"
                     /locus_tag="ACHUPB_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0759150.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13248..14696
                     /gene="cusS"
                     /locus_tag="ACHUPB_002358"
     CDS             13248..14696
                     /gene="cusS"
                     /locus_tag="ACHUPB_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0759151.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVSKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3541 a         3996 c         3640 g         3564 t
ORIGIN      
        1 caaaatatct gtttatttat acagtaagtc ctgtttcgga cttctgttgg caacactcgt
       61 tattgctcgt aaaacgaaat cgcccgcagc catcactgac cgcgggcgaa agtgtaaagc
      121 aggtgcccta ccatcctgac ctgacaaccg gatatgcggg gattactccc cgcacaacgg
      181 ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt ccttcaccgt
      241 aaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca aacacagcac
      301 tatgactgcc gcaagggcat atttcgtcag catgtttata ttgcctccat gcgaagaagg
      361 gactaccatc caaattgttc gggtttggag tggcagcccc agggttgata gaaatatctc
      421 ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct tcaggcaccc
      481 gccgttatct tacccttcct caacaaaatg gctgtttatt tatacagtaa atcctgtttc
      541 ggacttctca ctcctcattt aatcaactga agctgagccc gttttttctg catgcctcaa
      601 tcgtcaaaat tcttcgccat cagccagctc tgccataact tgccacattg aaaaagtagc
      661 caaattgctt cagtcaccat catcaaaaaa agaaacattc cagaacttcc gcatctcgcc
      721 cagccgctcc gacttctata ctgaatagaa aacgccaaca taagagaaac ctatgccatt
      781 acccgatttt catgtttctg aaccttttac cctcggtatt gaactggaaa tgcaggtggt
      841 taatccgccg ggctatgact taagccagga ctcttcaatg ctgattgacg cggttaaaaa
      901 taagatcacg gccggagagg taaagcacga tatcaccgaa agtatgctgg agctggcgac
      961 ggatgtttgc cgtgatatca accaggctgc cgggcaattt tcagcgatgc agaaagtcgt
     1021 attgcaggca gccgcagacc atcatctgga aatttgcggc ggtggcacac acccgtttca
     1081 gaaatggcag cgtcaggagg tatgcgataa cgagcgctat caacgaacgc tggaaaactt
     1141 tggctatctc atccagcagg cgaccgtttt tggtcagcat gtccatgttg gctgcgccag
     1201 tggcgatgac gccatttatt tgctgcacgg cttgtcacgg tttgtgccgc actttatcgc
     1261 cctttccgcc gcgtcgccat atatgcaagg aacggatacg cgttttgcct cctcacgacc
     1321 gaatattttt tccgcctttc ccgataatgg cccgatgccg tgggtcagta actggcaaca
     1381 atttgaagcc ctgtttcgct gtttgagtta caccacgatg atcgacagca ttaaagatct
     1441 gcactgggat attcgcccca gtcctcattt tggcacggtg gaggttcggg tgatggatac
     1501 cccgttaacc cttagccacg cggtaaatat ggcgggatta attcaggcta ccgcccactg
     1561 gttactgaca gaacgcccgt tcaaacatca ggagaaagat tacctgctgt ataaattcaa
     1621 ccgtttccag gcctgtcgct atgggctgga aggcgtcatt accgatccgc acactggcga
     1681 tcgtcgaccg ctaacggaag acaccttgcg attgctggaa aaaatcgccc cttccgcaca
     1741 taaaattggt gcatcgagcg cgattgaggc cctgcatcgc caggtcgtca gcggtctgaa
     1801 tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc tcgctgattg ggctggtgaa
     1861 aaagcattgt gagatctggg ccgatgacta agtagggaat tgccttacag aagaatatcc
     1921 ctgacaatgg tgttttaact tacatttaac aatgttatga aacacccttt agaaaccttg
     1981 actaccgcag caggcatttt gctgatggct ttcctctctt gcctgctgct gcccgccccc
     2041 gcactggggc tgacgctggc acaaaaactg gtgaccatgt tccatctgat ggatcttagc
     2101 tagctttaca ctttattgtt ttgtctgtgg tttttagtgc tgggcgctat tgagtatttt
     2161 gttctgcgct ttatctggcg acgctggttc tcgctggcgg attaaacgtc gattatcgcc
     2221 gtacttcgcg gcatactttg cttattctcc ttcgcctaaa ggaacgttta tggataagca
     2281 atcactgcac gaaacggcga aacgcctggc ccttgagtta ccctttgtcg agctttgctg
     2341 gccttttggc ccggagttcg atgtttttaa aattggcggc aaaattttta tgctgtcgtc
     2401 ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat ccacaaaaat ccctgttaaa
     2461 tcagcagata tacccgagca ttaagccagg gtatcacatg aataaaaagc actggatttc
     2521 ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc gatctgatca acgattcgtg
     2581 gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa agagtgcgtc caggctaaag
     2641 cggaaatcta tagcgcattt ttctcgctta ccatttctcg ttgaaccttg taatctgctg
     2701 gcacgcaaaa ttactttcac atggagtctt tatggatatc atttctgtcg ccttaaagcg
     2761 tcattccact aaggcatttg atgccagtaa aaaacttacc ccggaacagg ccgagcagat
     2821 caaaactctc ctgcaataca gcccatccag caccaactcc cagccgtggc attttattgt
     2881 tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc gctgccggta attatgtgtt
     2941 caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg ttctgtgcaa aaaccgcgat
     3001 ggacgatgcc tggctgaagc tggttgttga ccaggaagat gctgatggcc gctttgccac
     3061 gccggaagcg aaagccgcga acgataaagg tcgcaagttc ttcgctgata tgcaccgtaa
     3121 agatctgcat gatgatgcag agtggatggc aaaacaggtt tatctcaacg tcggtaactt
     3181 cctgctcggc gtggcggctc tgggtctgga cgcggtaccc atcgaaggtt ttgacgccgc
     3241 catcctcgat gcagaatttg gtctgaaaga gaaaggctac accagtctgg tggttgttcc
     3301 ggtaggtcat cacagcgttg aagattttaa cgctacgctg ccgaaatctc gtctgccgca
     3361 aaacatcacc ttaaccgaag tgtaattctc tcttgccggg catctgcccg gctatttccc
     3421 ctcagattct cctgatttgc ataaccctgt ttcagccgtc atcataggct gctgttgtat
     3481 aaaggagacg ttatgcagga tttaatatcc caggttgaag atttagcggg tattgagatc
     3541 gatcacacca cctcgatggt gatgattttc ggtattattt ttctgaccgc cgtcgtggtg
     3601 catattattt tgcattgggt ggtactgcgg accttcgaaa aacgagccat cgccagttca
     3661 cggctttggt tgcaaatcat aacccagaat aaactcttcc accgtttagc ttttaccctg
     3721 caggggatta tcgtcaatat tcaggcggta ttctggctgc aaaaaggcac cgaagcggca
     3781 gatattctga ctacctgcgc gcagttgtgg atcatgatgt atgcgctgct ttcagtcttc
     3841 tcgttgctgg atgttatttt gaatctggcg cagaaattcc cggcggcatc tcagttaccg
     3901 ctgaaaggga tatttcaggg gattaaactg atcggcgcga tcctggtcgg cattttgatg
     3961 atctcgctgc tgattggtca gtcgccagcg attctgatca gcggtcttgg tgcaatggct
     4021 gccgtgctga tgttggtatt taaagatccg attcttggtc tggtggccgg tattcagctt
     4081 tccgcgaacg atatgctgaa actgggcgac tggctggaga tgccgaaata cggcgcagat
     4141 ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc gtaactggga caataccatt
     4201 accactattc ccacctggtc tctggtttct gactccttta aaaactggag cgggatgtca
     4261 gcatctggcg ggcgacgtat taagcgcagt atcagtattg atgtcaccag tattcgtttt
     4321 cttgatgaag acgaaatgca acgtctgaat aaagcgcatt tgttaaagcc ttatttaacc
     4381 agccgccatc aggaaattaa tgagtggaat cgccagcaag gttctacgga gtcggtatta
     4441 aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct atctgaacga atatctgcgt
     4501 aaccatccgc ggattcgtaa agatatgacc ttaatggtac gccaactggc tccaggtgat
     4561 aacggtttac cgctcgagat ctacgcgttt actaacaccg tggtgtggct ggaatatgaa
     4621 agcattcagg ctgatatatt cgatcacata tttgcgattg tcgaagagtt tggtctgcga
     4681 cttcatcagt cgccaaccgg caatgatatt cgctctctgg cgggtgcatt taagcagtaa
     4741 ttaaaaaaac cgctctcatc gaatggatga gagcggacat cgttttgtcg gatgtagcgt
     4801 gaatgcctta tttccgacgc agcgttttaa atgccacaaa caggaataca atccacaccg
     4861 gcagcaggat cgctgacaag cgcatatcat ccatcgtgca catcagcagc aaaatcatgc
     4921 cgaggaaggc aatgcagaga tagtttccga acggatagag cagcgcctta aactgtgttt
     4981 cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg cgccagacag atcataatcc
     5041 agttcaacag cagcgttgct accaccagcg ccatcagcag accaaacgct ttttgcggca
     5101 gcagatagtt gattaacacc accagcgaag tgatcgctcc ggaaagcatc agcgagttaa
     5161 tcggcacacc gcgacggctg acgcgagtca aaaacttcgg cgcattaccc tgcacagaaa
     5221 ggccaaacag catgcggctg ttagagtaaa ccccgctgtt atacactgac agcgatgcta
     5281 ccagaatgac gaagttcagc gcagaagcta ccacgttgct gtcgagatta tggaaaatca
     5341 tcacaaacgg gctactgttg gatttcactt ccacccacgg atagagcgcc agtaaaacca
     5401 ccagtgaacc gatgtaaaac agcaggatgc gatacaccac ctgatttacc gcttttggaa
     5461 tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc aatcagctcc agaccgccga
     5521 aggagaacat aattaccgcc agcgacaaaa tcagcccatt ccagccggtg gcgaagaaac
     5581 caccgtagcg ccagaggttg tcgatactgg ctttctcgcc gccgtgacca gaaaacagca
     5641 gccacaggcc aaagccgatc ataccgatga ttgccagcac tttaatcaac gcaaaccaga
     5701 actcggtttc gccatataag cgcacgttca ccaggttaac ggcgttgatg ataataaaga
     5761 aggcggcagc ccaaatccac gttggaacat ccgggaacca gtactgcata tagatgcccg
     5821 cagcggtcag ctctgccatt cccaccagca cgaacattac ccagtagttc cagccagaga
     5881 ggaagcccgc aaacggtccc cagtatttat aggcaaagtg ggcaaatgaa ccggataccg
     5941 gctcttcgac caccatctcg ccaagctggc gcataatcag gaaagcgatg atcccggcga
     6001 cgccgtaacc cagcaataca gccggacccg ccatctgaat cgccggacca atgccaagaa
     6061 acagaccagt accaattgcg ccacccagcg caatcagttg aatatgacgg ttatgtaatc
     6121 cgcgatgaag cgtcggctct tgattcgacg cagtatcttc cgatacggtt gacgcgtttt
     6181 tcacgccttt cccctgtgtg tcttttttgt tgaggggcac cttttaacat ttagtgccca
     6241 tcgtcgcaag acacaatcca cacggttaaa cggggtatcc tgcttttatt tccgtacccg
     6301 atgtcggtgc agccacatca gcttatacgc cgccgggata ataaacagcg acagcaaagg
     6361 tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg ctcatcacct ctgaaccagc
     6421 ccccgttccc cacagaatcg gcagcagacc ggcgataatc accgccaccg tcatcgcttt
     6481 cgggcgcacg cgcagtaccg cgccgtgata caacgcctca tccagcttct gctcactgaa
     6541 tgtttgcgga ttattcaacg acggctcggc ctctatggcg tgacgtaaat acatcagcat
     6601 caccacgcca aattcggcgg cgaccccggc aagggcaata aaaccggtgc ccgttgccac
     6661 agaaagatga aagcccatcc accacagcaa ccagatgccg cccaccagcg caaacggtac
     6721 gctgctgata atcagcaacg cttcgcccac ccgacggaat gccagataca acagcacgaa
     6781 gatgatcatc agtgtcatcg gcaccatgag tttcagctta tggttggcgc gctccagtag
     6841 ctcgaactgc ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat
     6901 cgctttttgc aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat caatataaat
     6961 ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac ggtccggtag agactttaac
     7021 gtcggccacg tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gtagctggcg
     7081 cagtgcctgc gggctatcgc gccagctttg cggataacgc agattaatgg gataacgggc
     7141 aatcccttcc accgtttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg
     7201 cacatccgcc accgtcatac cgtaacgcgc ggctttttca cggttaatct caacgttgat
     7261 atagcgccca ccttccagac gctcggctag agcagaagcc acgcttggca ccgttcgcgc
     7321 tacttcttca atttgctcag ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt
     7381 aatgccgatg gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg
     7441 cacccacaga ttcgccagcc ccggcagccg cacggtgtta tccagttcct caatgatttt
     7501 gtccatcgtc atgccaggcc gccactgatc ctgcggctta agctggatgg tcgtttcaac
     7561 catctcgagc ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg
     7621 cgccacttca ggtacgctca taattagctt gtcggttttt tgcagcatac tcgccgcctc
     7681 tgctgcggaa atccccggca gcgtcgatgg catatacaac aagtcgcctt cattgatctg
     7741 cggtaaaaat tccccgccaa ctttattgag cggccagaga accgtcagca ccgaaagcgc
     7801 tgccaccaac agcgtggttt tcggccagtg cagtactttc agcaatagag gatgataaac
     7861 acgaatcaaa aagcgattga gcgggttact gctttccggc ggaattttgc cacggatcca
     7921 gtaccccatc aggataggga tcactacgat cgccagcagc gccgcgcccg ccatcgcata
     7981 ggttttagtg aacgccaacg ggccaaacag acgcccttcc tgcccttcca gggtgaagat
     8041 cgggataaac gacaacgtga taatcagcag actgataaat agcgccggac ccacttcaac
     8101 agatgcatcg gtgatcacct gccagcgcgt tttattatcc agcgtggcgt caggatgctg
     8161 gtgctgccac tcttccagcc gtttatgtgc attttcgatc atgacgatag cggcatcgac
     8221 catcgccccg acggcaatcg caatgccgcc caacgacata atattggcat tcagtccctg
     8281 gaagtgcatg acaataaaag caatacacaa ccccagcggc aacgaaataa tcgccaccag
     8341 cgccgagcgc acatgccaga gaaacagcgc acagactacc gccaccacga taaactcttc
     8401 cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg agctggctgc gatcgtatgt
     8461 tgtaactatc tccacgcctt ccggcagact acttttcagc gtttccagtt tgtccttcac
     8521 ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg atcaccaccc cgcccgccac
     8581 ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc tccgggccaa tctggacctt
     8641 cgcaacatca cgcagataaa cgggcacgcc attttctctg gcttttaaaa cgatgtgatt
     8701 aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc atatattccg cttccgccag
     8761 ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc agcgcgcttt ttacttcggc
     8821 tagactgatg ccatattgcg ccaggcgctg gggatcgata accacctgat actctttcac
     8881 cacgccgccc accgacgcca cttccgcaac gtcagggatg gtttttagct catatttgag
     8941 aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc ttaccgctgc gatccaccag
     9001 tgcatattca tagatccagc caacacccgt ggcatctggt cccagctcgg cgctgactcc
     9061 cgcaggcagc ttaccctgta cctggttgag gtactccagc acccgcgaac gcgcccagta
     9121 cggatcggta ccatcttcga aaatgacata cacataagag tctccaaact gcgagaaacc
     9181 gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg gttagcggat aagtcacctg
     9241 attttcaacg atttgcggtg cctgaccggg atagctggtt ttaataatca cctgcacatc
     9301 ggagagatcc ggcagcgcat ccactggcgt gttaatgatg gtccaggtgc cccagatgct
     9361 cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc accgagcgac gaataatcca
     9421 ttcaatcatt ggttattccc tcaatgcgca ttggtagcac tttcagagcg catccgctcc
     9481 agtgcgccag aaatattggc ttcggaatca atcaggaaca ggccgctgga aaccaccttt
     9541 tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt gcgatgcctg gaaaacggca
     9601 acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa tcacccgctg ttcgttgccg
     9661 gtatcaatca gcgcctgtga cggaatgagc agcatcggtt cgctggcggt gttgagttgc
     9721 aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt tgtcgacttc cagacgcagc
     9781 tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg tccatttgcg gatggtgagt
     9841 gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg aggcatcttt caccagccag
     9901 gcgatagact ccgggatcgc agcagtgacc cacaccgggt ccataccctg aattttcgct
     9961 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
    10021 tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggcggcga
    10081 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
    10141 gccgtaccgc cggtttcgcg cagcagtaaa tactcactct gcgcttccac ccagtcagga
    10201 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
    10261 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
    10321 ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc cgcgcgtgac ggtagccgtt
    10381 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
    10441 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
    10501 aaccgcgtat tggggtacat cgggtcgtac cagaataaga ttttacgttc tgcggtcgac
    10561 gttttttctg cgggtggttc agcctttgca aaccaggtaa aacctgccgc agaaataata
    10621 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
    10681 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
    10741 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
    10801 atagtcatct ccggccagtt cacggcagca atcggatcgt gatggatggt gatttttttg
    10861 ctttccagat caataccctt taccacgcca gtggcgctaa taacctgcgg ttgtacttcg
    10921 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
    10981 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
    11041 gctgccaacc gccccctaag gcggtataca aagaaatttc gttaacctga cgggcataat
    11101 tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
    11161 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcaaagta atttgcagcg
    11221 acgccagata acgctgctgg gcgctgattt gatcgttcag gctttgacgt aatgccagcg
    11281 catctgccac ttctttaaag gcgttctgga ttttctgttc ataattcact accgactgct
    11341 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
    11401 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gaagacagat
    11461 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
    11521 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
    11581 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
    11641 tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc caataacagt tgcaatgcat
    11701 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
    11761 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
    11821 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
    11881 acgccagttg ctgattgaaa tagctttgcg cgacattaga aaccagcaga atatgcaccg
    11941 cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc ggcttcgctc atgttcttta
    12001 agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc ggtcgaaaac tcccgtgtcg
    12061 tggcggtatt ccctttaaga ttaccgctcc agctgccgct gccctcgcca ttgagctgtg
    12121 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
    12181 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
    12241 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc tgctgcgtta accaagccgt
    12301 tctggctgag tgagaactgc tgcggcacgg gcattgccgt acgctgataa tccggtgcca
    12361 gtgaacaacc ggttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
    12421 gctcataatt tctggtgatt ttataccgcc aactttactc gccaggctct gattttccgg
    12481 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
    12541 ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa ctgttgattg tcgaagatga
    12601 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
    12661 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
    12721 cctcgatatt atgctgccgg acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
    12781 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
    12841 ggggctggag ttgggggccg atgactatct ggtgaagccg ttcgcttttg ctgaactgct
    12901 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
    12961 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
    13021 cactctgacc agtaaagagt ttactctgct ggagtttttt ctccgccatc agggcgaagt
    13081 gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg aatttcgaca gcgacactaa
    13141 cgccattgat gtggcggtga agcggctacg cggcaaaatc gacaacgact ttgagccgaa
    13201 gctgattcag accgtgcgcg gcgtgggcta tatgcttgag gtgccggatg gtcagtaagc
    13261 catttcagcg cccgttttcg ctggcaaccc gtctgacctt ttttatcagc ctggccacca
    13321 tcgcggcgtt tttcgccttt gcatggatca tgatccactc agtgaaagtg cattttgccg
    13381 agcaggatat taatgattta aaagaaatta gcgccaccct tgaacgggta cttaatcatc
    13441 ctgacgagac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
    13501 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
    13561 cggatatccg cgagtttacg cgtgacgcca tacccgataa agacgctcag ggtggcgagg
    13621 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
    13681 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
    13741 atacgctcta catcgcgctt tcgatcgatt ttcatcttca ttacataaat gatttgatga
    13801 ataaacttat tatgaccgca tctgtcatca gcatcctgat cgtctttatc gtgctattgg
    13861 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
    13921 cgaaagatct cgacgttcgc cttgacccgc agaccgagcc cattgagctg gaacagctgg
    13981 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttacccgc cagtccaatt
    14041 tctcagcgga tatcgctcac gaaattcgca cgccgattac caatctcatt acgcaaacgg
    14101 aaatcgccct cagccagtct cgcagccaga aggagctgga agatgtgctc tactctaatc
    14161 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
    14221 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcagcaaag
    14281 tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt ggagttgcga tttgttggcg
    14341 acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg ggcgttaagc aacctgcttt
    14401 ctaatgccct gcgttatacg ccaaccagag agacaattgt agtgcgctgt cagacggtcg
    14461 atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
    14521 cgcgattgtt tgaccgtttc taccgcgttg acccttcccg ccagcgaaaa ggcgaaggta
    14581 gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcag
    14641 taacgtcaga tgcgcggggg acaaggtttg tgattatgct gccggagaga gagtgattat
    14701 taagttcaaa ttcagggcgt ggtgcatcac ctcctggtat t
//
LOCUS       DAZOZD010000097        14555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOZD010000097 DAZOZD010000000
VERSION     DAZOZD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.097"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..293
                     /locus_tag="ACHUPB_002359"
     CDS             <1..293
                     /locus_tag="ACHUPB_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HFR0759152.1"
                     /translation="KAFHILERLEALATEEEISPEKLVEFSRVILRRKNDMERLTSGA
                     PSLSVRRTLCCSFCNKSQYAVKKLIAGDAVFICDECVDVCNRIIRGEKEGSA"
     gene            290..811
                     /locus_tag="ACHUPB_002360"
     CDS             290..811
                     /locus_tag="ACHUPB_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759153.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGRIGSTPSVEV
                     QSIQAGFDWDAGKVMIFPAQPLTTLTPEQVADITDSVRKGQSWHAYQEYKKHKEQLEK
                     LSMELEAAKQREKDLFMENVRLKSGIAGLIHLGIRYADVEVMKIAGDAQLSTPCTDSI
                     INSIAAGIRIKGE"
     gene            813..1076
                     /locus_tag="ACHUPB_002361"
     CDS             813..1076
                     /locus_tag="ACHUPB_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759154.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDERILFPSSVESTAG"
     gene            1087..1473
                     /locus_tag="ACHUPB_002362"
     CDS             1087..1473
                     /locus_tag="ACHUPB_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759155.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPVPVVPEEKPMPNPLSMYA
                     VDAVAAIAEVRGWNACRAAMLQGKGE"
     gene            1475..2050
                     /locus_tag="ACHUPB_002363"
     CDS             1475..2050
                     /locus_tag="ACHUPB_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0759156.1"
                     /translation="MTTITDKKQYPGEQYLNELITNIEFAARAPVEVVRAMAAELQKR
                     READSAEPASNHEELPLDYLQGQKDGLEWAAQLAEANHPQTGDWLYDDPLDLAKAIRK
                     GPDIPEFAGPHPVTPDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGITFR
                     RGDVTIKPGNGIEQATHWMPLPEPPQESKSE"
     gene            2043..2264
                     /locus_tag="ACHUPB_002364"
     CDS             2043..2264
                     /locus_tag="ACHUPB_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759157.1"
                     /translation="MNKAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRCYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            2261..3115
                     /locus_tag="ACHUPB_002365"
     CDS             2261..3115
                     /locus_tag="ACHUPB_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759158.1"
                     /translation="MMHKSVAGEFQKEVDNTTVLLDDILSILALLETGDWSEHCTKTE
                     LGGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSNRVE
                     KVDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWGECTRAAY
                     VRAKDNGELTRVVTNHPNNELKDHQIRELVNELRDIAVTYHGTQQLREKIARAVNNSL
                     KGGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLRAMEK
                     YGDKWEVL"
     gene            3129..3653
                     /locus_tag="ACHUPB_002366"
     CDS             3129..3653
                     /locus_tag="ACHUPB_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFR0759159.1"
                     /translation="MTKFGKLEAHLLTRNYRYDVHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRAHGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPNEVSHLIFSHSQ"
     gene            3826..4215
                     /locus_tag="ACHUPB_002367"
     CDS             3826..4215
                     /locus_tag="ACHUPB_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFR0759160.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKSPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(4253..5353)
                     /gene="apbC"
                     /locus_tag="ACHUPB_002368"
     CDS             complement(4253..5353)
                     /gene="apbC"
                     /locus_tag="ACHUPB_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0759161.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            5511..7544
                     /gene="metG"
                     /locus_tag="ACHUPB_002369"
     CDS             5511..7544
                     /gene="metG"
                     /locus_tag="ACHUPB_002369"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0759162.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKCMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLTSELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADEP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            7706..8005
                     /locus_tag="ACHUPB_002370"
     CDS             7706..8005
                     /locus_tag="ACHUPB_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759163.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIEYGCI
                     VHAGLQDDDWKDELRRYGISEGAIANIQKVLDVGFNSVHFDCGAPVVEGLECWQW"
     gene            8042..8227
                     /locus_tag="ACHUPB_002371"
     CDS             8042..8227
                     /locus_tag="ACHUPB_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759164.1"
                     /translation="MLKEDSYLLKALEMSGVEPTVKNAHDIYLQFSTELKQLINKYGV
                     NDSSVKAMIDDELDHLI"
     gene            9802..10017
                     /locus_tag="ACHUPB_002372"
     CDS             9802..10017
                     /locus_tag="ACHUPB_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759165.1"
                     /translation="MKMPVTVEVWGVDSLAECLDAVGPELYRKLWSFVPAEGESPKGK
                     DIWHLLSEDEKRELVGAVHSEFPDDQD"
     gene            10156..10485
                     /locus_tag="ACHUPB_002373"
     CDS             10156..10485
                     /locus_tag="ACHUPB_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759166.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     DFLAIFA"
     gene            10640..10951
                     /locus_tag="ACHUPB_002374"
     CDS             10640..10951
                     /locus_tag="ACHUPB_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759167.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            11077..11472
                     /locus_tag="ACHUPB_002375"
     CDS             11077..11472
                     /locus_tag="ACHUPB_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759168.1"
                     /translation="MSVKRENLTVDVYYASESAEGKNVAKITVVTYNTETGTEVQAST
                     IVRKGDTSGGEYEAQYQSIFDATDPLLLKIESYFRKVNKEVFETMMNMVNTVFASSLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            11591..11965
                     /locus_tag="ACHUPB_002376"
     CDS             11591..11965
                     /locus_tag="ACHUPB_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759169.1"
                     /translation="MGLDIYIETQPKNDLNNDASRKKVAYFRKFNALFGWMERNVGEV
                     KNCELLELTMNDICALKAHLMHMNESNCEEYLPTCEGFFFGSQEYDEGYWHDVGELKK
                     LVEELIKNHDFHNNRLTFCAWW"
     gene            12316..12798
                     /locus_tag="ACHUPB_002377"
     CDS             12316..12798
                     /locus_tag="ACHUPB_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759170.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(13417..13644)
                     /locus_tag="ACHUPB_002378"
     CDS             complement(13417..13644)
                     /locus_tag="ACHUPB_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759171.1"
                     /translation="MVIFISGVNINNHTLVYDIAGLAGYALSSEVVDETTFKININDV
                     EHRARAGINEADVTLMLQEFLNAGFNIRLEK"
     gene            complement(13665..13868)
                     /locus_tag="ACHUPB_002379"
     CDS             complement(13665..13868)
                     /locus_tag="ACHUPB_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759172.1"
                     /translation="MVSYDKIRAEYRAKYRAYKLELINDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLHNSI"
     gene            complement(13918..14553)
                     /locus_tag="ACHUPB_002380"
     CDS             complement(13918..14553)
                     /locus_tag="ACHUPB_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759173.1"
                     /translation="MSKKSIIEAASIVSDELREKADLATQTYNEHYKNGTHTKADKAN
                     MQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSLEKLVY
                     LVKSIKAGKCVYSVADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACEIKLDA
                     GYTQANQLINLCERLGLVEKVKGMGSAKAGTQQYRFIKNDFYNYLADAFKA"
BASE COUNT         4122 a         3272 c         3467 g         3694 t
ORIGIN      
        1 aaaaagcctt ccacatactc gaacgattag aggcgcttgc tacggaggag gagatatccc
       61 cggagaaact ggttgagttc agccgtgtga tattgcgtcg caagaacgat atggagcggc
      121 tgacatctgg cgctccatcc ttatcagtca ggcgaacact ttgttgcagc ttctgcaaca
      181 aatcccagta cgccgtcaaa aagttaattg ctggggacgc cgttttcatc tgcgacgagt
      241 gtgtggatgt gtgcaacaga attatccggg gagagaaaga gggatcagca tgaaattttc
      301 caaattttct gagttggtga atcgtatttt gtccaacaac cacagccatc gtcgcgatat
      361 ggatgtaacg atcgttgttc attcgcctgg tcgcatcggt tcaacaccat cagttgaggt
      421 tcagtcaatt caggcgggtt ttgattggga tgccgggaaa gtgatgattt ttccagcaca
      481 gccactgacc acgctaacac cggagcaggt tgctgatatc actgatagtg tgcgcaaagg
      541 tcagtcttgg cacgcgtatc aggaatacaa gaagcataaa gagcagttgg aaaaattgtc
      601 gatggagttg gaagccgcta aacagcggga aaaagatctg tttatggaaa atgttcgact
      661 taagtcaggt atagccggtc tgatacacct cggtattcga tatgcggatg ttgaggtcat
      721 gaaaattgct ggagatgccc agctttctac tccatgcact gacagcatca taaacagcat
      781 tgcagcaggc attcgcatca aaggagagtg atatggcaac tttaacaaaa aaagaacggg
      841 catggttgaa cgaattacag gacgttcttg atcgctgccc atcaccgaaa aaaattggtt
      901 tttacaccat tggcgataaa agcatttacc tgtatgacct gcgccgcatg gatgaaatca
      961 tggaggctct tgataatcgt tcgtcgatgg attggtgtgt tgctgtccat gatatgaatg
     1021 ccggatttga tgaaaggatt ttgttcccct catcagttga aagcactgcg ggttaaggag
     1081 taacacatga ccactattac caaagagcga ctactgacaa tcaaacagtg gcgcgaaaca
     1141 tacggacctg gtagcaacgt tgtactgcca gcagaagaag cggaagaact ggcacgaatt
     1201 gctctggcat cgctggaagc aaaaccaata ggtgcattcc acattgcaga acagcaagtt
     1261 gacggcacaa gtgactacct caaggatgga gaatggccta ttgataatgg aattattgag
     1321 gtctacgccg ctccacccgt tccagtagta ccggaagaaa aaccaatgcc taatcctctt
     1381 agcatgtacg cggttgatgc tgttgccgct attgcagagg tgagaggctg gaatgcctgc
     1441 cgcgctgcta tgcttcaggg aaaaggagag tgatatgacc actataaccg ataagaaaca
     1501 gtatccaggc gagcaatatc ttaatgagct gatcaccaac atagagtttg ctgcaagggc
     1561 accagttgaa gtcgtgagag cgatggcagc agagctacag aagcggcgcg aagctgatag
     1621 tgcagaacct gcaagtaatc atgaagagtt gccgcttgat tatctccaag gtcaaaaaga
     1681 tggtcttgaa tgggctgcgc agcttgcaga agcaaatcac ccacaaactg gcgactggct
     1741 ttacgatgac ccgctggatc tggctaaagc tatcagaaaa ggtcctgaca ttcccgagtt
     1801 cgctggaccc catccggtaa ctccggatgg ttggataagc tgtagtgagc gaatgcctga
     1861 aaagggccag aacgtgctta tttcggtgaa tttcgatagc tctctggttg aaccgctaat
     1921 atgctccgca cgctataccg gaatcacctt tcggcgcgga gatgtaacga ttaagccggg
     1981 taatggtatt gagcaagcaa ctcactggat gccgctacca gaacctccgc aggagtcgaa
     2041 gagtgaataa agcagagtta tttcagaaaa tatcggctct cgcgactgaa tgccacgcta
     2101 tagcatctga gcttgatgtt ggcgatgaac gaaccgagat gttcgaaata tacagtgtgc
     2161 tgcgcaatct ctgtcggcgt tgctacgcca ctcaagtagg gcgaatgact aacccactac
     2221 tctcatcctg tgatgaggat gactcggatg aggatgacga atgatgcata aatcagtagc
     2281 cggtgagttt cagaaggaag tcgataatac taccgttcta ttggacgata ttttaagcat
     2341 tctcgcgctt cttgagactg gcgattggtc agaacattgc actaaaacgg agttaggcgg
     2401 tcggcttgaa agagagatta cacgacttat tggtgatgcg caggaagcta cagtcactag
     2461 ttatgagtta atagctgaag cctggcgttt gatggatgga caagatccta aaaccagcga
     2521 ttggcatagc aaggcttcga agtatttaaa ttccaatagg gtagaaaaag ttgatgatgt
     2581 taaaccgaag ccagtagacc acggtttccg cgataactgc gaatgctcta gttgccagac
     2641 cacggcccgt atttgttccg aattgacaga taagtccagc ctaatctacg aagttaatgt
     2701 aggcggtaat acatggggcg aatgcacaag agctgcatac gtaagagcaa aagacaatgg
     2761 ggaattaacc agagttgtta ccaatcaccc aaataatgag cttaaagatc accaaattcg
     2821 agaactagtg aatgagttgc gggatattgc ggttacgtat cacggaacgc agcaattgcg
     2881 ggagaaaatt gccagagcag tgaacaactc gctcaaaggt ggacaatccc aaccgaaacg
     2941 tatacgaatt acacatatag gtgaaaacgg aacagaaatc ccccaatttt ctgttgggga
     3001 catctttgat gtacatggag aagagtttgg cgagcctcac atcactatgc ctagtggcca
     3061 tgtttattgg ctaagagcta tggagaaata tggtgataaa tgggaagtgt tatgagggat
     3121 gcgaagttat gactaaattt ggaaagctgg aagctcattt gctaactcgt aactaccgct
     3181 atgatgtgca tccatataga cagtggatcg atgaaagcga aggagttgtt acattccctc
     3241 tatacaggtt tgatgggatg ttggccgggt atcagacata cagaccaggc gctcctaagc
     3301 agcatagcaa tccgaagatg gcccggtatt tcacacgagc gcacggcaga cagctactat
     3361 ggggaaccta tcttccttta aaagatggac cgatatggat aactgagtcg atttttaaaa
     3421 gtgcagcagt gcacaacgcg ggcggaaatt cgtgggcgct gttaggttcg actttctcag
     3481 caggattacg tcgtcaactg gcaatgctgc cgtatgactt tcgcgtaata ggtgacaatg
     3541 atgcggctgg tgaatcactt gtgaagtctt tcggaaaagg ttttgtggcc ccggatcttg
     3601 acgagctaca accaaatgaa gtgtctcatt tgatttttag ccatagccaa taagcatccc
     3661 ttctccatta aggccactag ttgtggcctt aaacaatttg ttttctacct tttcttgttc
     3721 gagaaaataa cgttcctttg tctggtgcta tgtaccgatc agttgcactg agtgagcttc
     3781 tttcatgtat actgtgtaaa tgaacagtat tgagggcaaa acgctatggg cttcccttct
     3841 cctgcggcgg attatgctga gagccgtatt tctcttgatc agcagataat tagacatcct
     3901 tcagcgacct acttcatgag agcagctgat agtcatcatc gtgagggaat attacagggt
     3961 gcattgctgg tggttgattc ctcacttacc ccggttgatg gttctctgct tgtatgcgct
     4021 ctggatgggg aatatcgcgt aaaaagatac cggaagtctc cacgtcagca tctggaggat
     4081 ttaagaaccg gtaagaagga agcattgcca aaggatgacg atggatgcac gggcagcaat
     4141 gccgtgtttg gtgtgatcac tcacattatc aacgacgcaa gaagtggcga gtttgatgat
     4201 tgtcctgtga tgtaggagaa ctgattaggc ggtgcaatgc accgcctttt tatcacactg
     4261 cgcggaatgc gatttcgcca ggtattactt caccttgcca atacatttgg gcagcaacgc
     4321 gatctgcgag gtcacgataa atagccgtaa attcgctatc tggacgacta ataacggttg
     4381 gtgttccgtt atccagatct tcacgaagag agatatgaag tggcatttgg cctaacaact
     4441 gcgtgttgta tttctcggcc agtttctgtg ctccaccggt gccaaaaatt ggctcgtgat
     4501 gaccgcagtt actgcaaata tgcacactca tgttttcaac gatacccagt accggcactt
     4561 cgactttttc gaacatcaca atgcctttct tcgcatcgat cagcgcgatg tcttgcggcg
     4621 tagttaccac aaccgcacca gttacaggaa tgttctgcgc cagcgtcaat tgaatatcac
     4681 cagtgcccgg tggcatatcg agaacgagat agtccagatc aggccacagt gtttcctgca
     4741 acatctgcat cagcgccttg ctcgccatcg gtccacgcca caccattgca ttgtcatcgg
     4801 tgaccagata accaatagag ttggttgcca ggccatgaga catgataggt gccatgtgag
     4861 taccgtccgg tgaggttgga cgttggtttt ccgcgcccag catggttgga attgatggac
     4921 catagatatc ggcatccaaa ataccaactt tcgcaccttc agcagccaaa gccagtgcca
     4981 agtttactgc tgtggaggat ttacccacgc cgcccttgcc tgagctgacg gcgataatgt
     5041 tcttaacgcc attaatgcct ggttggtttt tgacgcgctt aagcgtggca atgttgtacg
     5101 acagcttcca gtcaatagcc tttgcgccag tgatacggag cagatcacca ctacattgct
     5161 ctttcaggtc ttcaaaaggc ttattccaca cgaaaggcat gattagttcg acatgcagtg
     5221 tgtcatccat caacgcaaca tggtgtaacg ctttaagcgt agtcaagttg tgtttcaggg
     5281 ttgggtgctg aaaattagcc agcgtaccgg ctaccattgc tctcagggca tccggcgatt
     5341 tggactcgct catcccgtct cctttatttt aatttgcgca attgtcgcct tgtagtgtac
     5401 tccagctacg acatttaatc atttatgaga aatgctgtta tcacatggca gacataaggc
     5461 cattttgtta ctatcaagcc ccttttcact acaaagaagt aatgcctact atgacccaag
     5521 tcgcgaagaa aattctggtg acgtgcgcgc tgccgtacgc taacggctca atccacctcg
     5581 gccatatgct ggagcacatc caggctgatg tctgggttcg ttaccagcga atgcgcggcc
     5641 acgaggttaa tttcatctgt gccgacgatg cccacggtac gccaatcatg ctgaaagcac
     5701 agcagcttgg tatcacaccg gaacagatga ttggcgaaat gagtcaggaa catcagactg
     5761 atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc gaagagaacc
     5821 gtcagttgtc cgagcttatc tactctcgcc tgaaagaaaa cggttttatt aaaaaccgca
     5881 ccatctcaca gctgtacgat ccggaaaaat gcatgttcct gccggaccgt tttgtaaaag
     5941 gcacctgccc gaaatgtaaa tccccggatc aatacggcga taactgcgaa gtctgcggcg
     6001 cgacctacag cccgactgaa ctgatcgagc cgaaatcggt ggtttctggc gctacgccgg
     6061 taatgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa atgttgcagg
     6121 catggacccg cagcggtgcg ttgcaggagc aggtggcaaa taaaatgcag gagtggtttg
     6181 aatctggcct gcaacagtgg gatatctccc gcgacgcccc ttacttcggt tttgaaattc
     6241 cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc gccgattggc tacatgggtt
     6301 ctttcaagaa tctgtgcgac aagcgcggcg acagcgtaag cttcgacgaa tactggaaga
     6361 aagactccac cgccgagctg taccacttca tcggcaaaga tatcgtttac ttccacagtc
     6421 tgttctggcc tgccatgctg gaaggcagca acttccgcaa gccgaccaac ctgtttgttc
     6481 acggctatgt gacggtgaac ggcgcgaaga tgtccaagtc tcgcggcacc tttattaaag
     6541 ccagcacctg gctgaatcat tttgacgctg acagcctgcg ttactactac actgcgaaac
     6601 tctcttcgcg cattgatgat atcgatctca acctggaaga tttcgttcag cgcgtgaatg
     6661 ccgatatcgt gaacaaagtg gttaacctgg cctcccgtaa tgcaggcttt atcaacaagc
     6721 gttttgacgg cgtgctgaca agcgaactgg ctgacccgca actgtacaaa acctttactg
     6781 atgccgctga agtgattggc gaagcatggg aaagccgcga atttggtaaa gctatccgtg
     6841 aaatcatggc attggctgac ctggctaatc gctatgtcga tgaacaggct ccgtgggtgg
     6901 tggcgaagca ggaaggtcgc gatgcagatc tgcaggcgat ttgctctatg ggcattaacc
     6961 tgttccgcgt gctgatgacg tacctgaaac cggtactgcc gaaactgact gaacgtgcag
     7021 aagcattcct gaatacagaa ctgacctggg atggtatcca gcaaccgctt ctgggccata
     7081 aagtgaatcc gttcaaggca ctgtataacc gtatcgatat gaaacaggtg gaagcactgg
     7141 tggaagcatc caaagaagaa gtgaaagcca cagctgcacc ggtaactggc ccactggctg
     7201 acgaaccgat tcaggaaacc atcacctttg acgacttcgc caaagttgac ctgcgtgtgg
     7261 cgctgattga aaacgcggag tttgtcgaag gttctgacaa actgcttcgc ctgacgctgg
     7321 atctcggtgg tgaaaagcgt aatgtcttct caggcatccg ttccgcttat ccagatccac
     7381 aggcactgat tggtcgtcac accattatgg tggctaacct ggcaccacgt aaaatgcgct
     7441 tcggtatctc tgaaggcatg gtgatggctg ccggtcctgg cgggaaagat atcttcctgt
     7501 taagcccgga tgctggtgct aaacctggcc accaggttaa gtaattaaac aacctctctt
     7561 cgcaatgccc ggcaacacgc cgggcatttt catatagaca caatccctcc tgatttacac
     7621 aagccctaaa acaatttgtt ttctaccttt tgttgattga gataataagc cacataagaa
     7681 aacaacttgt tttggagtag ttgaaatgtt gaacattacc caagcctacc aggtggcatt
     7741 gatcagttca tctcatacat cagaggaaga tgctcgccga ttcatgatcg caagcaatga
     7801 gctggattgg gtttcacaaa ttgagtacgg ttgcattgtc cacgccgggt tacaggacga
     7861 tgactggaaa gatgagttgc gtcgatatgg tatatccgag ggtgcgattg cgaacattca
     7921 gaaagtactt gatgtaggct ttaactctgt acattttgat tgtggcgcac cggtcgttga
     7981 agggcttgag tgctggcaat ggtaatccgg caatcgcttc atactagata acggagaatt
     8041 tatgcttaaa gaagactcat atctactaaa ggcgcttgaa atgagtggcg ttgaacctac
     8101 agtgaaaaac gctcacgata tctaccttca gttttcgaca gagctgaaac agctcataaa
     8161 taaatatgga gtgaacgact ccagcgtcaa agccatgata gatgatgaat tagaccatct
     8221 catctaatca aaccgttgat aatggcggtc acaatttgat tcttttctat aagaacccgt
     8281 tgatcgccac tactaccctc ccataccagc aatcacatca ttaaatatca ataggttatg
     8341 gttgtttata ccaataagaa aacaactaaa taacagatca acgaaacatc aacgctttct
     8401 cccgatttgg tctaaaaaat tgacgttatc acacatcact tcttataccc gtaattctac
     8461 gcagcagata gcgccacagc gtccaaattt caccacagac gactcaacac actaccaaca
     8521 aaaacacacg ctcaaaatcg ctcccgttgc gttacacaac catataaaca acttattttc
     8581 aggcataaca aaacaaccaa ataacacata tgccattacc caaaacaacc aacctcttta
     8641 tggcaggcta ccgaaaagac ccacctcttc tccccaggct accggacaac caacctaact
     8701 ttcacaggca atccgaaaac attcatacgc gcgataccca cgacacgcca ttacccaaac
     8761 agcgaagaaa cacctgaacc gctgaaaccc taacgaagcc tgtaaccgca aggtttctct
     8821 caatcccaaa agacacgaaa cataacaatc acgggaaacg ccattaccca atacacgaat
     8881 aactacaaaa gccttcacac actccagaca aacaactcaa acacaataac gaaagcaatc
     8941 gcccacctgc cattactcca tacacggaga agaacaatca ccaaaacgac gagaacaccc
     9001 ccatagaaga accaccgcaa aactaacgaa accacataca ccgacagaga aaacaacaca
     9061 cccaattcac caacagaaat agtcgccgta tagaacattc tggaaggagt gagcattaac
     9121 gcctatagag aggttgagac agataaaaca caacatgtat agcgaagaag ccaggtatgt
     9181 ataagtggag agggaaggag gggggtcgcc tcctttttcg tatttattca actcctgatt
     9241 ttatcccccg taacaccccc tacggtcagc cttcggtcca tacagggaaa aggttgcatc
     9301 cagctacaga aacggctggg ttgccttcgg gggaacggct ggggattttc agggaaacgg
     9361 tcgagttgcc tgtgtggatt tcgggaaaag gctcgatgcc ggttctggaa cggcggcgta
     9421 ccggtatagc gagagagaga gagttgcagc tacccacctg taatgtgcgg gaagaagaga
     9481 ggcgctcacg tgcaggctga cttgcttacc gctggattcc ctctgaatgc cagatacagc
     9541 gtaattgtgc cagacagcca gcgggtcaca atcgatttca gtaggttttc ggggagagga
     9601 ttattctacc caccagcccc gcggcgcgcg tagagcagaa tatcgtaaat actggctctc
     9661 gtgtcgttcc cggatattca ctgcccagcc tggctttccc gacataaaat cctttttcgc
     9721 ctttatctta ttgcgataat aacaccaaca agtaaacagc atatttagac gttatagaaa
     9781 cgaagtgagg atgaagagat gattaagatg cctgtgacgg ttgaggtgtg gggcgtggat
     9841 tccctggctg agtgtctgga tgcagtgggg ccggagttgt accgcaagtt gtggtcgttc
     9901 gtcccggcag aaggggaatc gcccaaaggg aaggatatct ggcacctgct gagtgaagat
     9961 gaaaagcggg agctggtggg cgcggtacac agcgagttcc cggacgacca agattaagag
    10021 tgaggccacc agcacggtgg cttttgtcgc tattaacctc ggggcagcgc agcaccgtaa
    10081 cgatatactt tgtggccgtt ttcttactgg tattgtttac gtaattcaga aaacaacatg
    10141 tttaaggatt gctttatgtt tgcaaatatc gacatcaacc aaattaagaa attgactcaa
    10201 aaagagtttg accagtttta tgagctggaa ggttggtctt ccactctgat taattcaaga
    10261 tgggtgctgg agctgatgac tcgtgatgac gcacctgctt tgatgatttg cgacatgggt
    10321 gaagatgctg actttatgga tatgagcgaa ttttgtgtgg acacatacaa ccgcagccag
    10381 aagtactact tcacatgcga tagcgaaaat gacgtgattt ctaaggtcta tcttcacctc
    10441 gtccagcatt gggacgttca ggacttcctt gcgatattcg cgtaagccca accaaagcca
    10501 gcattgctgg cttaacttca tctgtagccg cagggaagaa cctgcatatc gctcatgcgc
    10561 ctgatcaata tgctttgtag ccgttttctt actggtatta tttacgccat tgagaaaaca
    10621 agttgtttac ggagttgtga tgaaaaaatc attggttctt ggtctggaca aagaccagaa
    10681 gagaaaagag aagcctgcac tggttgctca attaactttg ctggacatcg ttgccaatgg
    10741 aacctctatt cgtctgttcc gtgaaacagc ggtgtctttc gataaaaaca cctttactcg
    10801 ttatgtaatg aatgttcgtc gccagcgtgg aaaaggctgg atggcatttc aaagaatgtg
    10861 gccggaacat cagctcgaac tggctttgat ggaagttaac cgcgtggccc agcaagagat
    10921 tcagagagca tcagtgatgg caatagcctg ataatgtgca agtggtaatt agtcgacagt
    10981 acgacagccc cgccatcctt acggggtttt ttgtattgta agtacatacc tatgtctata
    11041 aaatagaaaa ataaccaaga cactatggag agtgatatga gcgttaaacg agaaaacctg
    11101 acagttgatg tctactatgc ttcagaaagc gccgaaggta agaatgtagc taaaatcacc
    11161 gtcgttacgt acaacaccga aactggaact gaagttcagg caagtactat tgtgcgtaaa
    11221 ggagatacat caggcgggga atatgaggcg caatatcagt ccatttttga tgcaacggac
    11281 ccgttgttgt tgaaaattga aagttacttc cgtaaggtga ataaagaggt gtttgaaacc
    11341 atgatgaata tggttaacac cgtattcgct tccagtctga acactaacac tacctggatt
    11401 ggtcagtatg gtctgcgcat tacctccggc attcctgctg acaccttaat ccctgaaagc
    11461 gtattcgctt aatcctcttt gaatggcgcg taaaccgcgc cattttctta tgcccgataa
    11521 caatttgttt tctgccttat ctgatttgtg aaaatgcttt cactgaaagc aacttgataa
    11581 ggaaaccatc atgggacttg atatctacat tgagacgcaa ccaaaaaacg atctgaataa
    11641 cgatgcatcc agaaagaagg ttgcttactt ccgtaagttc aatgctctct ttgggtggat
    11701 ggaacgcaac gtaggtgaag tcaaaaattg tgagctttta gaattaacga tgaatgacat
    11761 ttgtgctctg aaagctcatt tgatgcacat gaacgaaagt aactgcgaag agtacttacc
    11821 tacctgtgaa ggttttttct tcggcagtca ggagtacgat gaagggtact ggcatgacgt
    11881 gggggagttg aaaaaacttg tggaagagct gattaagaac cacgactttc acaataacag
    11941 actgaccttc tgcgcctggt ggtaaatatg ggcgatttca agaaacgcct gaaggaaaga
    12001 gccgagatag tcagaaagca aaacgcctct tctgtcatca gatacgcaag gcagtttagt
    12061 cgcaacaaca aatcagttga ggaaaagatc cttagcgtaa tcggtcgata attattaagg
    12121 ccaccactgc tggtggcctt aaatgaccat cctgtttccc gcaggctaaa aacaccaacc
    12181 tcttacctcc aggcaaccga caaacccacc tgttcccgtc cggctaccgc aactttccac
    12241 tttgacgcct tattcgtaca acgataatta acgccaacaa gaaaacaatt tgttatttac
    12301 gataaggaat taatcatgaa ttttatcgct actgtaaacg cacccgcaca tggcaatatc
    12361 gctgtaacgt tctctgacat tgaaaaacga gtacttggtg catggcgcga caatgagacg
    12421 gtagaactgt cagcacaaga aaaatgcatt attgcacgcg acatcattgg caatcgtcgt
    12481 tactcgcggg tatttgagaa agcgtatgtg gtaaattctg gattcggaac gttcgtcttt
    12541 ccggtgcgct ccgggcgatt ctgccagtcc aagctgattg agttcgctac gcagatttct
    12601 gtctggatta aaactcaatc gtcgttcaaa ttttccgacg atgaagcagt atcgcagggg
    12661 atgcggatcg ccaacaatgc aattaaatgc aaaaacatta cgtataccgc tggcgttgat
    12721 acatggaaac tgttttgcgc taactttatg ctgaatgtat acgcaagcaa ccgcatccac
    12781 atccttgatg gcgtgtaact gagaagaggg ccagtaacgg ccctttctct atagccacca
    12841 gatgccgcag ggaaattttc agaaacggcg aggaacgtat tcatgaaccg acggggaacg
    12901 gccaggattt tttcgggaaa cggctgcatt cgcctttatg tagaaaaaac atcgggaagc
    12961 tggtggaatc caaccagcgg ttgtcggaca ggtgagcggg gaaaattatg atgactttcg
    13021 tcgcctgaga catccagatt tctttcgtag cgtaatcgca tacgtgatta agtggtgtga
    13081 ttatgtgaaa aatcacgcgc acataatacg cgagcggata cggaacaaaa caaaatgccg
    13141 atccgcgccg acaaataaac gcggatcaca tagcaagact aaaagccaat gattaactat
    13201 gcgctatagc gcaatataac gcgttttaag cgcgttaatg tgttaagtaa tgggtatgta
    13261 ctggcaagga tataaaagcg cgtctatggc gttattttgg cgcttatttt tatgttgttg
    13321 gagtgagtca aagacaataa aaaacgcgcc atcattggcg cgttatggtg ggagtattgg
    13381 aaacgaaaaa agcgcccact atgggcgcta ttgtttttat ttttctaagc gaatattaaa
    13441 accagcgttt aaaaattctt gaagcattaa agtaacatct gcttcattga ttcctgcgcg
    13501 cgctctatgt tctacgtcat tgatgtttat tttaaacgtt gtttcatcaa ccacttcaga
    13561 acttagagca tatccagcta aaccagcaat atcataaaca agagtatgat tattgatatt
    13621 tacgccagag atgaaaataa ccatgattaa gctccttaaa aaaattaaat agaattatga
    13681 agttttccga ttaatgcgct taaacgtaag tattcacgtt tacgcttgca atctcgtttg
    13741 ctgttaagca aatcagaaaa cgtaaaattt aattggtcgc gttgcgcgat taaatcattg
    13801 attaattcta gtttataagc gcgatacttt gcgcgatatt ccgcgcggat cttgtcatag
    13861 ctgaccattt tttaaagctc ctttaaagcg cccatagtgg gcgcttgatt ccattaatta
    13921 cgctttgaaa gcgtcagcta gataattgta gaaatcattc ttgataaagc gatattgctg
    13981 tgtaccagct ttggcgcttc ccattccttt gactttctca acaagtccga gacgttcgca
    14041 aagattgatt agctggttag cttgagtata gccagcgtcc aatttaattt cacacgcttt
    14101 tttggcttca ttcattaaat cgaaaacagc gccattcgtg aacgtgtcga tctcgtcgtt
    14161 aatcatatcg attaaagcga atacacgaga tccagacata tcagcgacgg aataaacaca
    14221 ttttccagct ttaattgatt taaccagata aaccagtttt tccagtgaat aactattagt
    14281 catagcgtca cgaaaaaata cttctggcgc ttgtttgctt gctttaatcg catagtagaa
    14341 aacagagcat aatttttcgt cttctacagc gtttacgacg ttgttgatga agtaagcaag
    14401 tttagtggtc gcagcttgca tatttgcttt gtctgcttta gtgtgcgtac catttttata
    14461 atgttcgtta tatgtttgag ttgccagatc tgctttttcg cgcagttcgt cggatacaat
    14521 agatgcagct tcaatgatgg actttttaga aatga
//
LOCUS       DAZOZD010000098        14374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOZD010000098 DAZOZD010000000
VERSION     DAZOZD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.098"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            95..637
                     /locus_tag="ACHUPB_002381"
     CDS             95..637
                     /locus_tag="ACHUPB_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0759174.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            737..1939
                     /locus_tag="ACHUPB_002382"
     CDS             737..1939
                     /locus_tag="ACHUPB_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0759175.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2159..2941
                     /locus_tag="ACHUPB_002383"
     CDS             2159..2941
                     /locus_tag="ACHUPB_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR0759176.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2956..4160
                     /gene="rhmD"
                     /locus_tag="ACHUPB_002384"
                     /pseudo
     CDS             2956..4160
                     /gene="rhmD"
                     /locus_tag="ACHUPB_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4217..5506
                     /locus_tag="ACHUPB_002385"
     CDS             4217..5506
                     /locus_tag="ACHUPB_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0759177.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5524..6327
                     /gene="yfaU"
                     /locus_tag="ACHUPB_002386"
     CDS             5524..6327
                     /gene="yfaU"
                     /locus_tag="ACHUPB_002386"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR0759178.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6368..7270)
                     /locus_tag="ACHUPB_002387"
     CDS             complement(6368..7270)
                     /locus_tag="ACHUPB_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0759179.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7463..8653)
                     /gene="glpC"
                     /locus_tag="ACHUPB_002388"
     CDS             complement(7463..8653)
                     /gene="glpC"
                     /locus_tag="ACHUPB_002388"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0759180.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8650..9909)
                     /gene="glpB"
                     /locus_tag="ACHUPB_002389"
     CDS             complement(8650..9909)
                     /gene="glpB"
                     /locus_tag="ACHUPB_002389"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0759181.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9899..11527)
                     /gene="glpA"
                     /locus_tag="ACHUPB_002390"
     CDS             complement(9899..11527)
                     /gene="glpA"
                     /locus_tag="ACHUPB_002390"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0759182.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11800..13158
                     /gene="glpT"
                     /locus_tag="ACHUPB_002391"
     CDS             11800..13158
                     /gene="glpT"
                     /locus_tag="ACHUPB_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0759183.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13163..14239
                     /gene="glpQ"
                     /locus_tag="ACHUPB_002392"
     CDS             13163..14239
                     /gene="glpQ"
                     /locus_tag="ACHUPB_002392"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0759184.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14281..>14374)
                     /locus_tag="ACHUPB_002393"
     CDS             complement(14281..>14374)
                     /locus_tag="ACHUPB_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0759185.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
BASE COUNT         3273 a         3550 c         3961 g         3590 t
ORIGIN      
        1 agatcccttt tttcgctcat tgaatcctct gtgccaggca gtacaatccc gcagctttgg
       61 gaattttgat taattgcttg tgggaaaggg gataatgaaa aaaattgcgc tagcaggtct
      121 ggccggaatg ctgttggttt ctgcatcggt caatgcaatg agcatcagcg gtcaggcggg
      181 taaagaatac accaatattg gtgtcggttt tgggactgaa tcgacgggcc tggcgttaag
      241 cggtaactgg acacataacg acgacgacgg tgacgtcgct ggcgtggggc tggggttgaa
      301 tctgcctctc gggccgttaa tggcgaccgt tggcggaaaa ggcgtttaca ccaacccgaa
      361 ttacggcgat gaaggttatg ccgcagcggt aggaggtggt ttgcagtgga aaattggcaa
      421 cagcttccgt ttgtttggcg agtattacta ctctccggat tcactctcca gcggtattaa
      481 aagttatgag gaagcgaatg ctggcgcgcg ttacaccatt atgcatccag tcagtattga
      541 ggcgggttat cgctacctga atctgtcggg taaagacggt aaccgcgaca acgccgtggc
      601 tgacggcccg tatgttgggg ttaacgccag tttctgatcc cttccggcgc ggcattctgt
      661 cgcgccattc agtttcttac tacgccttat ctccctttgc gttatagtga ttcgataaca
      721 aaagcaggag ataaccatgt taaaagtgga aatgttatcc accggggatg aagtgttaca
      781 cgggcaaatc gttgacacta acgctgcctg gctggccgat tttttctttc atcaggggtt
      841 gccattatct cgccgcaata cggtggggga taaccttgat gacttagtca ccattcttcg
      901 cgaacgtagt cagcacgccg atgtgctgat cgttaacggc gggctgggac cgaccagtga
      961 tgatttaagt gcactcgccg ctgcgacagc aaaaggtgaa ggcctggtgc tgcatgaagc
     1021 ctggctcaaa gagatggaac gctatttcca cgaacgtgga cgagtaatgg caccgagcaa
     1081 ccgtaaacaa gcggagctgc ctgccagtgc tgaatttatc aataacccgg taggcaccgc
     1141 ctgtggtttt gccgtgcggc ttaatcgttg cctgatgttc tttactcccg gcgtaccgtc
     1201 agaatttaag gtgatggtcg agcacgaaat cctgccgcgc ctgcgcgagc gtttttcttt
     1261 accacagtcg ccggtttgtc tgcgtttaac tacctttggt cgttcggaaa gcgatctggc
     1321 acaaagcctg gacactctac aactgccgcc gggcgtaaca atgggctatc gctcctcaat
     1381 gccgatcatc gaactgaaac tcaccggacc ggcaagcgag caacaggcga tggaaaaact
     1441 gtggctggac gttaaacgtg ttgccggaca gagcgtgatt ttcgaaggca ccgaaggact
     1501 gcccgcgcag atcagtcgcg aattgcaaaa ccgccagttc agcctgacgt tgagcgagca
     1561 attcaccggt ggtttattgg ctttgcaact ttctcgcgca ggtgctccat tgctggcgtg
     1621 tgaagtggtt ccttcacagg aagaaaccct ggcgcaaact gcgcactgga ttacagaacg
     1681 gcgggccaac cattttgccg ggctggcact ggctgtttcg ggtttcgaga acgagcatct
     1741 caactttgcg ctagccacgc cagacggcac tttcgctctg cgtgtgcgtt tcagcactac
     1801 acgctacagc ctggctatcc gtcaggaagt gtgcgcaatg atggcactga atatgctgcg
     1861 ccgttggtta aacggccagg atatcgccag tgagcatggc tggattgagg ttgttgagtc
     1921 catgacctta tctgtctgat tccgccactc tgtgccggtt tactgcaccg gcacattccc
     1981 cctccggcaa aacttgtcca acctgttgta ctcaataaga aaaaatccgt gcactgattc
     2041 acaaatttgt gcgtaatttc tccagtccgc tggacgagga gcgggatttg atgcgcttca
     2101 caattttact ccccctgagc aacgagactg attccagtat ctaagactgg agtccagtat
     2161 gttggaatca agcaaagttc ccgccctgac acgggctatt gatattctca atttgattgc
     2221 ccgcattggc ccctgtagtg ctgcgacgat cattgacaca ttgggaatcc ctaaaagcac
     2281 ggcctatttg ctgcttaatg aactcaggcg tcagcgtttt cttagccttg atcaccagga
     2341 aaatttttgt ttgtggacca ggctggtgga gctttctggt catgcgttaa gcaagatgga
     2401 tctccgggaa ctggctcgac cgcgtttgac gcaactgatg gataccaccg ggctgttatg
     2461 tcacctgggg attattgata acggcagcgc ttattacatt ttaaaagtgg agtcatcagc
     2521 cactatcagc gtgcgttccc atgaaggaaa aagcctttcg ctttatcgct ccggaatagg
     2581 taaatgcctg cttgcctggc aacctgcagc ggtacagcaa agtattatcg aagggctggt
     2641 ctgggagcag gcgacaccca caacgattac tcatccacag caacttcacg aagaactggc
     2701 gcgtattcgc cgtcagggct ggagctatga caacggcgaa gattaccccg atgtccgttg
     2761 tgtcgctgcg ccggtgttta acgccaataa cgagcttacc gctgcgatct cggtggtcgg
     2821 tacccgctta caaatcaatg aagaataccg tgattatctg gcgggcaagg ccattgcctg
     2881 cgccagggat atttcacgtc tgttgggatg gaaaagtccc tttgacttac aggcctcata
     2941 acaatggaga acatcatgac cctaccaaaa atcaaacagg ttcgcgcctg gtttactggc
     3001 ggtgcgacag cagaaaaagg tgctggcgcg gtgattatca cgaccagggg gcgaatcact
     3061 ggattgacga tcatattgcc accccgatga gtaaataccg cgattacgag cagtcacgcc
     3121 agtcatttgg cattaacgtt cttggcacgt tgattgttga agtcgaagca gaaaacggcc
     3181 agaccggatt cgctgtttcg acagccggtg aaatgggctg ttttattgtc gaaaaacatc
     3241 ttaaccgttt cattgagggt aaatgtgtca gtgatatcaa actgatccat gatcaaatgc
     3301 tcaatgccac cctgtattac tccggctctg gtggcctggt gatgaacacg atttcctgtg
     3361 tcgatctggc tctgtgggat ctgttcggca aagtggtcgg gcttccggtt tataaacttt
     3421 taggcggcgc agttcgtgat gagattcagt tctacgccac aggcgcgcgt ccggatctgg
     3481 caaaagagat gggctttatc ggtggcaaaa tgccgacgca ctgggggcca catgatggcg
     3541 atgcggggat ccgcaaagat gccgttatgg tcgcggatat gcgtgaaaaa tgcggtgagg
     3601 atttctggtt aatgctcgac tgctggatga gtcaggacgt gaactatgcc accaaactgg
     3661 cccacgcttg cgcgccctat aacctgaaat ggatcgaaga gtgcctgccg ccacagcagt
     3721 atgaaggtta tcgcgaactg aaacgcaacg cgccagtcgg aatgatggtc accagcggtg
     3781 agcaccacgg cacactgcaa tcttttcgta cgctttcaga aaccggtatc gacattatgc
     3841 agccggatgt tggctggtgc ggtggtttaa ccacgctggt ggaaattgcc gcaatcgcca
     3901 aatccagggg gcaactggtg gtgccgcacg gttcgtctgt ttactctcac catgcggtga
     3961 tcaccttcac caatacgcca ttcagcgaat tcctgatgac cagcccggat tgttcaacga
     4021 tgcgtccgca gtttgacccg attctgctca atgagccggt tccggtgaat ggtcgtattc
     4081 ataaatcagt gcttgataaa cccggttttg gcgtcgaact caatcgtgac tgcaatttga
     4141 aacgccccta cagccactaa tcactccggg cgttgctgtg acaacggtaa cgcccacata
     4201 ttctaaggag ctttttatga gcaccgcttt gcttgacgcc gtggtgaaga aaaaccgtgt
     4261 gcgtttaatt ccgtttatgt tggcgctgta tgtgctggcg tttctcgacc gttcgaatat
     4321 cggttttgcc aaacagacct accagattga taccgggttg agtaatgaag cttatgcgct
     4381 gggagcaggc attttctttg tggtatatgc gtttctgggt gttccggcga atcttttgat
     4441 gcgcaaactg ggggccagaa cctggattgg tacgacaaca ctgctgtggg gatttctttc
     4501 ggcagccatg gcatgggccg atactgaagc gaaatttctg atagttcgca ctctgcttgg
     4561 tgctgcggag gccggatttt tccctggtat gatttatctc acctcgcaat ggtttccgca
     4621 gcgtaatcgc gccagcatta tggggctgtt ctatatgggc gcaccgctgg cgttaacact
     4681 gggatcaccg ctttccggcg cgctgttgga gatgcatgga tttatggggc atcccggctg
     4741 gttctggatg tttgtgattg aaggattgtt ggcagtcggt gctggggtat tcacattctt
     4801 ttggcttgat gacacaccgg agcaggcacg ttttctgagt aaacaagaaa aaacgttgct
     4861 tatcaatcaa ctggcaagtg aagaacaaca gaaagtgact tctcggctga gcgatgcgct
     4921 gcgtaatggc cgagtctggc aactagcgat tatctacctg accattcagg tggcggttta
     4981 cggattaatt ttcttcctgc cgacccaggt tgcggcattg ctgggaacaa aagtgggctt
     5041 tacagcgtcg gtggtcaccg ccattccgtg ggttgcggcc ttgtttggga cctggcttat
     5101 tccgcgctat tccgataaaa ccggcgaacg gcgtaatgtc gcagcgctga cattactggc
     5161 ggcgggcatt ggtattggtc tgtccgggct gctttctcca gtaatggcga tcgtagcgct
     5221 gtgtgttgca gctatcgggt ttattgccgt gcagccggtg ttctggacga tgccgacaca
     5281 actgctttcc ggtacggcgc tggctgcggg gattggtttt gtaaacctgt ttggtgcagt
     5341 gggcgggttt attgccccga tcctgcgcgt gaaagcagaa acgttatttg ccagcgatgc
     5401 ggcaggatta ctgacactgg cagcggtggc ggtcatcggt tcgctgatta ttttcactct
     5461 gcgtgtaaat cgtactgttg cgcagaccga cgtggcacat cattaaatag gttaaggaac
     5521 acgatgaacg cattattaac caatcccttt aaagaacgtt tacgcaaggg cgaagtgcaa
     5581 attggtctgt ggttaagctc gactacttct tatatggcag aaattgccgc tacttccggt
     5641 tatgactggt tgctgattga cggggagcac gcgccaaaca ccattcagga tctttatcac
     5701 cagctacagg cggtagcgcc ctatgccagc caacccgtga tccgtccggt ggaaggcagt
     5761 aaatcgctga ttaaacaagt tctggatatt ggcgcgcaaa ctctattgat cccgatggtc
     5821 gatactgccg atcaggctcg tcaggtggcg tctgccacgc gctatcctcc ctacggtgag
     5881 cgtggtgttg gggccagtgt ggcacgggct gcgcgctggg gacgcattga gaattacatg
     5941 gcgcaagtca acgattcgct ttgtctgtta gtgcaggtgg aaagtaaaac ggcactggat
     6001 aacctggacg agatcctcga cgtcgaaggg attgatggcg tgtttattgg accagcggat
     6061 ctttctgcgt cgttgggcta cccggataac gccgggcacc cggaagtgca gcgaattatt
     6121 gaaaccagca ttcggcggat ccgtgctgcg ggtaaagcgg ctggttttct ggctgtggct
     6181 cctgatatgg cgcagcaatg cctggcgtgg ggagcgaact ttgtcgctgt tggcgttgac
     6241 acgatgctct acagcgatgc cctggatcaa cgactggcga tgtttaaatc aggcaaaaat
     6301 gggccacgca taaaaggtag ttattgatat caaaggccca tggggatcgg ctgtgggcct
     6361 gtgttaatta gtggttattc gctgccagat cggcttcgct tagctgggtg gccgcgagca
     6421 cctggtcacg atcaatgccc tgttccagca tgcgcaaagc aattttaatg gcttgttcat
     6481 gcataccttt ctgccagcca atttggtgtc cttcctgccg tagccgctca gcaatagtca
     6541 tcaatctctc cttttgtagt ggtgaacgtt cggaaatctc ctcaataaaa cgggtgaaac
     6601 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     6661 tgaagccttt aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc
     6721 gaatatgctt ttgaatcagt tcaagcagag ctatacgccg atgttgcatg atctcatcgt
     6781 caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt
     6841 gcggatcgtc aaactcatcc agccagttaa gtgagtacgg gtagggcgat gtttcgccat
     6901 gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc aggtgacgct
     6961 gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct gcagagcttt
     7021 gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg gaccacaaaa
     7081 catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc aggcgtaagg
     7141 tttgcaggtt gcaaagcttg cgcagtggtt ctggcaaatg tatttcgaga aaatcccgtg
     7201 cggtttcggg tgtgaacata aaggttttaa ataccgcatc atgcggggag gaggttgttg
     7261 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     7321 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     7381 gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt aaccacatgc
     7441 ttaccgggca tcagaaagga gtttaagcca gcgcctgggc cagtagcgta atcggatgtt
     7501 cgcagcgaag acttgtggac atctcaatct gccatttaca ggtttcgcag tcggtgacca
     7561 ccagatctgc gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg
     7621 tggggtagtt ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca
     7681 gcaccgttaa ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc
     7741 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     7801 gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat
     7861 cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg
     7921 tacaggttga ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg
     7981 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg
     8041 gtacgccgca gcatttttct ttgctgagca gttgtacacc gatacccatt gcgttgagca
     8101 ctttaattaa atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     8161 aagcgacctg gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgga
     8221 acgtgccgaa ggagtatttc ggtagcgtgc gacgatgatc gatttttaac gccgcatcaa
     8281 gcagctgccg caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg
     8341 aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
     8401 tgtcatattt tgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg
     8461 cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat
     8521 ctttcaaacg cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat
     8581 tcacccggct caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
     8641 tggtgtcgtt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
     8701 actgacggca caaacaccgc cgccgcatcc ttgggcgatg ggatcaaatc cgcccagcac
     8761 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctc
     8821 atcagtggtt acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca
     8881 ttcaccccgc gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt
     8941 acgttccgct accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg
     9001 acgtagcgga atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca
     9061 ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg
     9121 ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat
     9181 cagtgaacaa ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa
     9241 gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa
     9301 cagcggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt
     9361 ggcgttattg cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag
     9421 atccagttcg cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat
     9481 ccccactaca catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa
     9541 ccaggttgca cgcaaagtgc cgagcggggt aacgcgctga tgggccagtt ccacgctgcc
     9601 ctgcaattgc gcaccgcttt cagcgatcaa tgcctgcgcc tggcaagcga gatcgagcac
     9661 gcgttgtggt tcgagaagac tgtaaggatg ggctggtgcc tgctgacgca aagattccag
     9721 tccactgtga atgtctgtca ccggttgacc atcaggcaga tggctcagca aatccagcga
     9781 accggatgag aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg
     9841 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc
     9901 aaagcgcatc tttctgctcc ttctccagac cacacagtcc ctgataaacc cagcgggtaa
     9961 attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt
    10021 taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca
    10081 gcagtccggc agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg
    10141 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
    10201 cacccgctgt taccgcttcg cactcacata ccaggctacg gtgcagacga ccttcgctca
    10261 gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca
    10321 gggagatgac tttacgcaag gtaacttcag cgggttcctg tgaaccagga agtgccagat
    10381 cggcggtcgt acaggggcgc gtgttgccca gtttgcggca cacggcgtcg gtagcccatt
    10441 cagccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac
    10501 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt
    10561 cgtcatcgct ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg
    10621 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca
    10681 ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa
    10741 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat
    10801 gctggttaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc
    10861 gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta accacgacag
    10921 gcgcatgaag ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgcacaccg
    10981 tcgcgccttc acgaatcagt cccgtgactt catgagcggt aaggataacg gcaccgtgtt
    11041 ctttggcatc cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa
    11101 ctttcaccgc gccaatcaat gccgggttaa cggcaggttc gataatgcgc gcttgctgcg
    11161 ggtctatagc ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga
    11221 aggagaggtc atcttccggc agggtaataa acaggccgtt ggttggttca acgcagtgac
    11281 gtgcaatgcg tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg
    11341 ttaccgcata gcgcgcaccg ctgtgtagca ggccgtggtt acgcccggtg gcaccggttg
    11401 cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa
    11461 tcccggctcc cgttgcgccg ccgccaatga taatcacgtc acttgattgc gagtcgcgag
    11521 ttttcattgt tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa
    11581 ttgtttgatt tcgcgcatat tcgctcatga ttcgaaagtg aagcgtgatt tcatgcgtca
    11641 ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
    11701 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctctcact gaatcaggct
    11761 gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca
    11821 gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc
    11881 tggcaaattt tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac
    11941 tttgcgcttg ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt
    12001 gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg
    12061 gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg
    12121 ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg
    12181 ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac
    12241 tggtggtcgc agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc
    12301 ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcat
    12361 gcggcgctct atatgcctgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg
    12421 atgcgcgata ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat
    12481 ccggacgact ataacgaaaa agcggaacag gagctgacgg cgaagcaaat cttcatgcag
    12541 tacgtactgc cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg
    12601 ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg
    12661 ctggataaat cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcactctg
    12721 ctgtgcggct ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc
    12781 tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca
    12841 accgtcgata tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg
    12901 atcggtctgc atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt
    12961 accgggctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc
    13021 gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg
    13081 gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa
    13141 aaacgcaacg gaggctaatg gcatgaaatt gaagctgaaa aaccttagca tggcgatcat
    13201 gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt
    13261 catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat
    13321 ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca
    13381 tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc
    13441 ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa
    13501 gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtac agacttatcc
    13561 ggggcgtttc ccaatgggta agtccgactt ccgggggcac acctttgaag aagagattga
    13621 atttgttcag gggttaaatc actctaccgg gaaaaatatc ggtatctatc cagaaatcaa
    13681 agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct
    13741 gaagaaatat ggttacaccg gtaaggacga taaagtttat ttgcaatgtt ttgatgctga
    13801 tgagctgaag cgtattaaga atgagctgga acctaaaatg ggcatggatc tcaatctggt
    13861 acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg acggaagctg
    13921 ggttaattac agctatgact ggatgtttaa gccaggtgcc atgaagcaag tcgcggaata
    13981 tgccgacggt atcgggccgg attaccatat gttgattgag gaaacatcgc agccgggtaa
    14041 tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta
    14101 taccgtgcgg tcggataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc
    14161 tctgtataac aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa
    14221 attccttaat aaagagtaat gctactgcag gatggcgttc atgcgccatc ctgtctcata
    14281 tcaaattttt ccaatcgcca ctaattgttc catgcacatt tgtagtaaaa acagtggact
    14341 gccttctacc ggcttttctt tagccaacaa cgaa
//
LOCUS       DAZOZD010000099        14306 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOZD010000099 DAZOZD010000000
VERSION     DAZOZD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.099"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="ACHUPB_002394"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="ACHUPB_002394"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0759186.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHUPB_002395"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHUPB_002395"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0759187.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHUPB_002396"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHUPB_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR0759188.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="ACHUPB_002397"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="ACHUPB_002397"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0759189.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="ACHUPB_002398"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="ACHUPB_002398"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0759190.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHUPB_002399"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHUPB_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0759191.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHUPB_002400"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHUPB_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0759192.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHUPB_002401"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHUPB_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0759193.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="ACHUPB_002402"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="ACHUPB_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0759194.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="ACHUPB_002403"
     CDS             complement(13987..>14306)
                     /locus_tag="ACHUPB_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0759195.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAZOZD010000100        14091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOZD010000100 DAZOZD010000000
VERSION     DAZOZD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.100"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUPB_002404"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUPB_002404"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0759196.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUPB_002405"
     CDS             2273..2662
                     /locus_tag="ACHUPB_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0759197.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUPB_002406"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUPB_002406"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0759198.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUPB_002407"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUPB_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0759199.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUPB_002408"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUPB_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0759200.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUPB_002409"
     CDS             4687..5232
                     /locus_tag="ACHUPB_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0759201.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUPB_002410"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUPB_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUPB_002411"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUPB_002411"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0759202.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUPB_002412"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUPB_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0759203.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUPB_002413"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUPB_002413"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0759204.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUPB_002414"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUPB_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0759205.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUPB_002415"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUPB_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0759206.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUPB_002416"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUPB_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0759207.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUPB_002417"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUPB_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0759208.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUPB_002418"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUPB_002418"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0759209.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUPB_002419"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUPB_002419"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0759210.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAZOZD010000101        13814 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOZD010000101 DAZOZD010000000
VERSION     DAZOZD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.101"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUPB_002420"
     CDS             <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHUPB_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0759211.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="ACHUPB_002421"
     CDS             complement(984..1337)
                     /locus_tag="ACHUPB_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0759212.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUPB_002422"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHUPB_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0759213.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="ACHUPB_002423"
     CDS             complement(3165..3458)
                     /locus_tag="ACHUPB_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0759214.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUPB_002424"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHUPB_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0759215.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /locus_tag="ACHUPB_002425"
     CDS             complement(5012..5482)
                     /locus_tag="ACHUPB_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0759216.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUPB_002426"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="ACHUPB_002426"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0759217.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUPB_002427"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHUPB_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0759218.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUPB_002428"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="ACHUPB_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0759219.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUPB_002429"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHUPB_002429"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0759220.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="ACHUPB_002430"
     CDS             10838..11413
                     /locus_tag="ACHUPB_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0759221.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="ACHUPB_002431"
     tRNA            11520..11595
                     /locus_tag="ACHUPB_002431"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            11838..11952
                     /locus_tag="ACHUPB_002432"
                     /pseudo
     CDS             11838..11952
                     /locus_tag="ACHUPB_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12212..12511
                     /locus_tag="ACHUPB_002433"
     CDS             12212..12511
                     /locus_tag="ACHUPB_002433"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR0759222.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUPB_002434"
     CDS             complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHUPB_002434"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR0759223.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUPB_002435"
     CDS             12694..13749
                     /gene="cadC"
                     /locus_tag="ACHUPB_002435"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR0759224.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
BASE COUNT         3419 a         3496 c         3315 g         3584 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggta
//
LOCUS       DAZOZD010000102        13807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOZD010000102 DAZOZD010000000
VERSION     DAZOZD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.102"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUPB_002436"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHUPB_002436"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR0759225.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUPB_002437"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHUPB_002437"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0759226.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUPB_002438"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHUPB_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0759227.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUPB_002439"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHUPB_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0759228.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="ACHUPB_002440"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="ACHUPB_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUPB_002441"
     CDS             complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHUPB_002441"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0759229.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUPB_002442"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHUPB_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0759230.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUPB_002443"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHUPB_002443"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0759231.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUPB_002444"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHUPB_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR0759232.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUPB_002445"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHUPB_002445"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0759233.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUPB_002446"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHUPB_002446"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR0759234.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUPB_002447"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHUPB_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUPB_002448"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHUPB_002448"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0759235.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUPB_002449"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHUPB_002449"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0759236.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUPB_002450"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHUPB_002450"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0759237.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAZOZD010000103        13794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOZD010000103 DAZOZD010000000
VERSION     DAZOZD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.103"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..147)
                     /locus_tag="ACHUPB_002451"
     CDS             complement(<1..147)
                     /locus_tag="ACHUPB_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR0759238.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="ACHUPB_002452"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="ACHUPB_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="ACHUPB_002453"
                     /pseudo
     CDS             476..1021
                     /locus_tag="ACHUPB_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHUPB_002454"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHUPB_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0759239.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="ACHUPB_002455"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="ACHUPB_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0759240.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="ACHUPB_002456"
     CDS             3028..3201
                     /locus_tag="ACHUPB_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759241.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3227..3295
                     /gene="yncP"
                     /locus_tag="ACHUPB_002457"
     CDS             3227..3295
                     /gene="yncP"
                     /locus_tag="ACHUPB_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0759242.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHUPB_002458"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHUPB_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0759243.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="ACHUPB_002459"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="ACHUPB_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0759244.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="ACHUPB_002460"
     CDS             complement(5499..6422)
                     /locus_tag="ACHUPB_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0759245.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="ACHUPB_002461"
     CDS             6639..7982
                     /locus_tag="ACHUPB_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0759246.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="ACHUPB_002462"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="ACHUPB_002462"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0759247.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="ACHUPB_002463"
     CDS             10002..10226
                     /locus_tag="ACHUPB_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0759248.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="ACHUPB_002464"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="ACHUPB_002464"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0759249.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHUPB_002465"
     CDS             complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHUPB_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0759250.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="ACHUPB_002466"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="ACHUPB_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0759251.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="ACHUPB_002467"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="ACHUPB_002467"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0759252.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3574 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAZOZD010000104        13735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOZD010000104 DAZOZD010000000
VERSION     DAZOZD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.104"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="yojO"
                     /locus_tag="ACHUPB_002468"
     CDS             complement(<1..118)
                     /gene="yojO"
                     /locus_tag="ACHUPB_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0759253.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAI"
     gene            227..721
                     /gene="napF"
                     /locus_tag="ACHUPB_002469"
     CDS             227..721
                     /gene="napF"
                     /locus_tag="ACHUPB_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0759254.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            711..974
                     /gene="napD"
                     /locus_tag="ACHUPB_002470"
     CDS             711..974
                     /gene="napD"
                     /locus_tag="ACHUPB_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0759255.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            971..3457
                     /gene="napA"
                     /locus_tag="ACHUPB_002471"
     CDS             971..3457
                     /gene="napA"
                     /locus_tag="ACHUPB_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0759256.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3464..4159
                     /gene="napG"
                     /locus_tag="ACHUPB_002472"
     CDS             3464..4159
                     /gene="napG"
                     /locus_tag="ACHUPB_002472"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0759257.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4146..5009
                     /gene="napH"
                     /locus_tag="ACHUPB_002473"
     CDS             4146..5009
                     /gene="napH"
                     /locus_tag="ACHUPB_002473"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0759258.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            5006..5455
                     /gene="napB"
                     /locus_tag="ACHUPB_002474"
     CDS             5006..5455
                     /gene="napB"
                     /locus_tag="ACHUPB_002474"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0759259.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5465..6067
                     /gene="napC"
                     /locus_tag="ACHUPB_002475"
     CDS             5465..6067
                     /gene="napC"
                     /locus_tag="ACHUPB_002475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0759260.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6080..6703
                     /gene="ccmA"
                     /locus_tag="ACHUPB_002476"
     CDS             6080..6703
                     /gene="ccmA"
                     /locus_tag="ACHUPB_002476"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0759261.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6700..7362
                     /gene="ccmB"
                     /locus_tag="ACHUPB_002477"
     CDS             6700..7362
                     /gene="ccmB"
                     /locus_tag="ACHUPB_002477"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0759262.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7404..8141
                     /gene="ccmC"
                     /locus_tag="ACHUPB_002478"
     CDS             7404..8141
                     /gene="ccmC"
                     /locus_tag="ACHUPB_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0759263.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8138..8347
                     /gene="ccmD"
                     /locus_tag="ACHUPB_002479"
     CDS             8138..8347
                     /gene="ccmD"
                     /locus_tag="ACHUPB_002479"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0759264.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8344..8823
                     /gene="ccmE"
                     /locus_tag="ACHUPB_002480"
     CDS             8344..8823
                     /gene="ccmE"
                     /locus_tag="ACHUPB_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0759265.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8820..10763
                     /gene="ccmF"
                     /locus_tag="ACHUPB_002481"
     CDS             8820..10763
                     /gene="ccmF"
                     /locus_tag="ACHUPB_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0759266.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10760..11317
                     /gene="dsbE"
                     /locus_tag="ACHUPB_002482"
     CDS             10760..11317
                     /gene="dsbE"
                     /locus_tag="ACHUPB_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0759267.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11314..12366
                     /gene="ccmH"
                     /locus_tag="ACHUPB_002483"
     CDS             11314..12366
                     /gene="ccmH"
                     /locus_tag="ACHUPB_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0759268.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12401..13048)
                     /gene="narP"
                     /locus_tag="ACHUPB_002484"
     CDS             complement(12401..13048)
                     /gene="narP"
                     /locus_tag="ACHUPB_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0759269.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13368..>13735
                     /locus_tag="ACHUPB_002485"
     CDS             13368..>13735
                     /locus_tag="ACHUPB_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0759270.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         2937 a         3458 c         4033 g         3307 t
ORIGIN      
        1 taatcgctat ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc
       61 acgcttcccg ccactctttt gatcctgcta caggttttac cccgatcggg gtatgcatct
      121 ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta tgcggtttat
      181 ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga agattgatgc
      241 atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc
      301 ctggtccggt gatgaatctc attttctgac ccattgcacc cgttgcgacg cctgtattaa
      361 tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa
      421 aaataacgag tgcagcttct gttacgcctg cgcgcaggct tgccccgaat cgctattttc
      481 tccgcgccac accagggcct gggatttgca gttcaccatt ggggacgcgt gcctggcgta
      541 tcagtcagtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa ttatctttcg
      601 cccgacgctc tccgggatct accagccgca acttaatagc caactttgca acggatgtgg
      661 agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc atgcacacta
      721 actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca gacatcagca
      781 cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt
      841 tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt gagtcagtac
      901 gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg
      961 aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc
     1021 tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga
     1081 agccattaaa tgggataaag cgccgtgccg tttctgcggt actggttgcg gcgttctggt
     1141 cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac cggttaaccg
     1201 tggcctgaac tgcattaagg gctatttcct gcccaagatc atgtacggta aagaccgttt
     1261 gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg aatttacccc
     1321 aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga
     1381 aaaagggccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct gggaaggtta
     1441 tgccgcgtcc aagctgttta aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg
     1501 tcactgtatg gcgtcggcag tagttggctt tatgcgtacc tttggtatgg atgagccgat
     1561 gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg caaacatggc
     1621 ggagatgcac ccgatcctct ggtcgcgcat cactaaccgt cgtctctcta atcagaacgt
     1681 caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg ataacggcat
     1741 catctttacg ccacagtctg acctggtgat cctgaactac atcgccaact atatcattca
     1801 aaacaatgcg ataaatcagg acttcttcag caaacacgtt aacctgcgca aaggggcgac
     1861 ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga agaatccagg
     1921 ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg ccgagtatac
     1981 gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac cagttagaac aactggcgca
     2041 gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct tcaaccagca
     2101 tactcgtggc gtgtgggcta acaacctggt ctacaacctg cacctgctga ccggcaaaat
     2161 ttcccagccg ggttgcggtc cgttctccct gaccgggcaa ccttctgcgt gtggtactgc
     2221 gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga ccaacgagaa
     2281 acaccgtgat atctgcgaga agaagtggaa tatcccgagc ggcaccattc cggcgaaaat
     2341 cggtctgcac gcggtggcac aagaccgtgc gctgaaagac ggcaaactga atgtttactg
     2401 gaccatgtgt accaacaaca tgcaggccgg gccgaacatc aatgaagagc gtatgccggg
     2461 ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg tatccgacag tcagtgcgct
     2521 ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag aaagagggtg cttacggtaa
     2581 cgccgaacgc cgtactcagt tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc
     2641 ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag tgtggccgga
     2701 agagctgcta gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag tgctttatgc
     2761 cacaccggaa gtgagcaaat tcccggtatc cgaactggcg gaagatcagc tgaacgatga
     2821 atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg catggtttgg
     2881 tcgcggtcac ggtcacgatc tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg
     2941 ctggccggtg gtgaacggta aagaaacgca gtggcgttac agcgaaggta acgacccgta
     3001 cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg gatggtaaag cggtgatctt
     3061 cgcgctgccg ttcgaaccgg cggcggaagc accggatgaa gagtacgacc tgtggctctc
     3121 taccggacgc gttctggagc actggcacac tggcagtatg actcgccgtg tgccggaact
     3181 gcaccgcgcc ttcccggaag cggtcttgtt tattcacccg ctggatgcga aagcgcgcga
     3241 tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt
     3301 tgaaacgcgc ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt tcttcgacgc
     3361 cgcacagctg gttaacaaac taacgctgga cgcgaccgat ccgctctcga aagagacgga
     3421 cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa
     3481 acctcaaaat ggtcgccgcc gctttctgcg cgatgttgtt cgcacagcag gcgggctggc
     3541 tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt
     3601 gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca
     3661 gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggctt ctggcctgtc
     3721 ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg aggacattcc
     3781 gtgcgccaaa gtgtgcccga gtggtgcgct ggatcgtgag atcgaatcga tcgacgacgc
     3841 gcggatgggg ctggcggtgc tggtggacca ggaaaactgt ctcaactttc aggggctgcg
     3901 ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc tggagctgga
     3961 gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca gcgacgcctg
     4021 taccggctgc ggtaagtgcg aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt
     4081 accgctgtca ctggcgaaag gggagttagg tcaccattac cgcttcggct ggctggaggg
     4141 gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggtgaaa aaaggctggt
     4201 ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt
     4261 ttttgagcgg tccgtggttt ggtgtgtgga tcttacacgg caactacagc tgtagcctgt
     4321 tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt gccagtggtc
     4381 atctgcctgc cacggtggcg ttgaccggtg cggtgataat cactgtgctc tacgccctgg
     4441 caggaaagcg attattttgc agctgggtct gcccgctgaa cccgattacc gacttagcga
     4501 actggttacg caggcgcttt gacctcaatc agtctgcgac gatccctcgc cacattcgct
     4561 acgtgctgtt ggtggtcatt ctgctgggat cagccttaac cggcacgctc atctgggaat
     4621 ggatcaaccc cgtttccctg atggggcgta gcctggtaat gggcttcggc agcggcgcgc
     4681 tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc tggtgcgggc
     4741 acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg attaccgttg
     4801 cggccagcga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt tgcccggaac
     4861 cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc accagccgcg
     4921 attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt acaataacta
     4981 cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca
     5041 atggacggcg atgctggccc tggtggtaag cggcgcggtt tgggcggcta atggcgtcga
     5101 ttttagccag tcgccggaag tatccgggac gcaggaaggg gccattcgga tgccaaaaga
     5161 gcaggatcgg atgccgctga actatgtgaa tcagccgcca atgatcccgc atagcgttga
     5221 aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta
     5281 tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg acggcaaagt
     5341 gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga
     5401 taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga aataagaggt
     5461 tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga aatggtggcg
     5521 tacccccagc cgtctggcgc tgggaacgct gctgttgatc ggttttgtcg gcggcatcgt
     5581 cttctggggc ggctttaaca ccggaatgga aaaagccaat accgaagagt tctgcattag
     5641 ctgccacgaa atgcgtaaca cggtgtatca ggaatacatg gattccgtgc actacaacaa
     5701 ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa
     5761 gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg gcgttattga
     5821 cacgccgcag aaatttgaag ctcatcgtct gacaatggca cagaatgagt ggcggcgcat
     5881 gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata tggatacaac
     5941 cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg
     6001 tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg
     6061 tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt gtgagcggga
     6121 tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt gggtacaaat
     6181 caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg
     6241 ccctgacgca ggcgaggttc tctggcaagg gcagcccttg catcaggtac gcgacagcta
     6301 ccatcaaaac ctgttatgga taggccatca gccggggatc aaaacccggc tgacggcgtt
     6361 agaaaatctg cacttttatc atcgcgatgg cgataccgca caatgtctgg aagccctggc
     6421 gcaggccggg cttgccggat tcgacgatat tccggtaaat caactttctg ccgggcaaca
     6481 acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga tcctcgacga
     6541 gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta tggcgcagca
     6601 tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg ttgctgaaag
     6661 taaaattcgc cgcatttcac tgacgcaaac gagggccgca tgatgttctg gcgcattttc
     6721 cgtcttgagc tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc
     6781 ttcctgattg taattaccct ttttccgctc agtatcggtc cggagccgca actgctggcg
     6841 cgtattgcac cgggcattat ctgggttgct gcgctgcttt catccttgct ggcgctggaa
     6901 cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt gttgccgtta
     6961 cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac cggtctgccg
     7021 ttactcatcc tttcgccact ggtagcaatg ctactgggaa tggatgttta tggctggcaa
     7081 gtgatggcgc tgacgctgct gctgggaacg cctacgcttg gctttctcgg tgcaccgggt
     7141 gtggcgttga cagtgggact taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg
     7201 ctgactatcc cattactcat ctttgccacc gccgcgatgg acgcggcttc catgcatttg
     7261 cccgttgacg ggtatctggc aattttaggc gcgttgctgg caggcaccgc gacattaagt
     7321 ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat tactgatttt
     7381 tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg atcccaccac
     7441 ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt gtggtcgtgc
     7501 ttaccgtcgg ctggatctgg ggattcggct ttgctccggc tgattatcag cagggaaata
     7561 gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc atttatgcat
     7621 caatggcagt ggcagcgttt attggccttg tctggcagat gaaaatggcc aacctggcgg
     7681 tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt accggctctg
     7741 catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgtctg acttctgaac
     7801 tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc
     7861 gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta
     7921 ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa
     7981 gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt
     8041 ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa aaacgccgtc
     8101 cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg
     8161 gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg tggtgatgac
     8221 cgttattccg ctggtggttt tggtcgtgca ctcggtgatg caacatcgcg caattctgcg
     8281 tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc
     8341 tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg
     8401 gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct cttttatacg
     8461 ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt
     8521 ttgcgcgttg gcgggatggt gatgctgggt agtgtgcagc gcgatcccaa ttcgctgaaa
     8581 gtgaccttca ctatttacga tgctgaaggc tcagtggatg tctcttacga aggcattttg
     8641 ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat
     8701 catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc gccagaagtt
     8761 gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga cccagcatca
     8821 tgatgccgga aattggaaac gggctgctgt gtctggcgtt agggattgcg ctgctgctgt
     8881 ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg catgatcgcg tcttcccgct
     8941 tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt
     9001 tcgtggtcaa cgactttacc gtcacctatg ttgccagcta ctccaatacc cagcttccgg
     9061 tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctactg ctgtgggtgc
     9121 tgctgatgag cggctggacc tttgcggtgg cgatttttag tcagcgtatt ccgctggata
     9181 ttgtggcccg cgtactggcg ataatgggga tggtcagcgt cggctttttg ctgttcattc
     9241 tctttacctc taacccattc tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc
     9301 ttaacccgct gttacaggac ccggggctga tcttccatcc gcctctgctt tatatggggt
     9361 acgtgggttt ctcggtggcg tttgcttttg ccattgcttc tttgctgagc gggcgtctgg
     9421 acagcactta tgcgcgtttt actcgcccgt ggacgctggc ggcgtggatt ttcctgacgc
     9481 tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggg ggctggtggt
     9541 tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact gcgctgatgc
     9601 actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta ctgctggcaa
     9661 tcagtgcctt ctcgttgtgt ctgttgggga ctttcctcgt gcgttccggc gtgctggtat
     9721 cggtacacgc gtttgcgtct gatccggcgc gcggtatgtt tatcctcgcc tttatggtgc
     9781 tggtgattgg aggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg
     9841 taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg
     9901 ccgcgatgct ggtggtgttg ctggggacgc tgctgccgct ggtgcacaag caactgggac
     9961 tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg ctgatggtgc
    10021 cgtttgcgct actgctgggt gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta
    10081 aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtct ctgctgttgc
    10141 cgtggctgtt tgagagcaaa gttgtggcga tgacggtgct cggcctggca atggcctgct
    10201 ggattgcggt gctggcaatt gcggaagctg cgctgcgtat ttcacgcggc acgaaaacca
    10261 ccttcagtta ttgggggatg gtggctgctc acctggggct ggcagtgaca attgttggca
    10321 ttgcttttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc ggcgatagca
    10381 tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact
    10441 ggcgtggcgg tgtggcgact atcggcgtaa cgcgagaggg taaaccggaa acagtgctgt
    10501 atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg gcgattgacg
    10561 gtggcatcac gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg
    10621 ctgtgcgtct ttactacaaa ccatttgttc gctggatttg ggcgggcggg ctgatgatgg
    10681 cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa
    10741 aaactgcgcc ggaggctgta tgaagcgcaa agtattgtta attccgttga ttatcttcct
    10801 ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg atgatccgac
    10861 caatctggaa tcggcgctca ttggcaagcc agtgccgaag tttcgcctcg aatcactgga
    10921 caatccgggg cagttttatc aggcggatgt gctgactcag ggcaaaccag tgctgcttaa
    10981 cgtctgggcg acctggtgtc cgacctgccg tgcggaacat caatatctga atcagctttc
    11041 tgcgcagggc atccgcgtag tcggcatgaa ctataaagac gatcgccaga aggcaatcag
    11101 ctggctgaaa gagctgggca atccttacgc gctaagcctg tttgatggcg atggcatgtt
    11161 agggctggat ctcggtgtct atggcgcgcc agaaacgttc cttattgacg gcaacggcat
    11221 cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc
    11281 gctgtgggag aaatacagta aggaggccgc acaatgaggt ttttattggg catgctgatg
    11341 ctgatgatct ccggctcagc gctggcgacc atagacgtgt tgcagtttaa agatgaagca
    11401 caggaacaac agttccgtca gctcactgaa gaactgcgct gcccgaaatg ccagaacaac
    11461 agcattgccg attccaactc gatgattgcc accgacctgc gccagaaagt gtatgaactg
    11521 atgcaggaag gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg ttacggcaac
    11581 ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt gctgccagta
    11641 gtagctattg gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgtg
    11701 gtgccggaag cgtttcctga acaaagcgtg ccggaaggta agcgtgccgg atatgtcgct
    11761 tatttgccgg gcattgtggt ggcgttaatt gtggctggcg tcagctacta ccagaccgga
    11821 aattatcaac aggtgaaaat ctggcagcag gccacggcac aggctccggc gttgctggac
    11881 agggcgctgg atccgaaagc tgatccgctc aacgaagaag agatgtcgcg ccttgcgctg
    11941 gggatgcgta ctcaactgca aaaaaatccg ggagatatag acggctggat tatgttgggc
    12001 cgcgttggca tggcgctggg taacgccagt atcgccaccg atgcatacgc tactgcgtat
    12061 cgcctcgatc cgaaaaacag tgatgccgca ctgggttatg ctgaagcgtt gacacgttca
    12121 tctgatccca acgacaaccg cctcggcggc gaactgctac gtcagctggt gagaacggac
    12181 catagcaata tccgtgtgct aagcatgtat gcgtttaatg cctttgagca gcagcgattt
    12241 ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa cgatactcgc
    12301 cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt
    12361 aaataaaaag aggcccgatg gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt
    12421 gcaggaacag aatggtggcc gccacgcgtg agcggacatt gagtttacgc agcagatttc
    12481 gaatatgaac ttttactgtc tgctcggaaa tattcaacac agaggcaatc tgtttatttg
    12541 acagcccctg tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg
    12601 gatcttcttc cgcgccaaac atttcacgtt cacgtaagta ctgattgacg cgttcgctaa
    12661 agactttgct gcctttcgct ccggcacgaa tcgcttccag caacacttcc gggtcgctgt
    12721 ctttcagcag ataaccgtct gcgcctgcgt ctatcagcgc aaagacatcg ctggaggcat
    12781 cggatacggt caggataata atttgcgcgg taacgccatc cctgcgcaag gcattgaggg
    12841 tatccaggcc actcatacct ttcatattga gatccagcaa gatcacgtcg atatccagtc
    12901 tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag
    12961 gatcaagctc cagtaactga cgaacaccgc gtcgcataag tggatgatca tccacaatca
    13021 tcacctgaaa aggcgttgct tcaggcatag tagtctcctg aggttttatt agaatcatta
    13081 tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa tgttacccca ttttatgtat
    13141 ggacatgtag tgaaaactac aaccttgccg ttcgctttgg catcattttc attataaaga
    13201 cggaattttg catcagcttc tcatcaagaa aaacaactaa aaaatcaatc acaatacttg
    13261 tgcatttctt taaacagttt ttaaaaactg actccgggta tggagctatg ggtattttct
    13321 gtacccaatg cttttaacag caattaattt cataggatga aagctcaatg catcaatctg
    13381 gttctgtttc tctttgtcgt tccgcaatat ctgttctggt ggctacagcg ttatattcac
    13441 ccatagcatt ggcatcaaca gttgagtatg gtgagacagt tgatggtgtt ggcctggaaa
    13501 aagatatcca gctggtttat gggaccgcca ataatacgaa aatcaatcct ggcggagaac
    13561 agcatataaa agaatttggt gtaagtagta atactgaaat taacggcggg tatcagtaca
    13621 ttgaaatgaa tggcactgca gaatactcag tattaaatga tggttatcaa attgttcaaa
    13681 tgggtggcgc ggtaaaccag actacgctca ataatggagt gctacaggtt tatgg
//
LOCUS       DAZOZD010000105        13483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOZD010000105 DAZOZD010000000
VERSION     DAZOZD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.105"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1704
                     /gene="adeD"
                     /locus_tag="ACHUPB_002486"
     CDS             <1..1704
                     /gene="adeD"
                     /locus_tag="ACHUPB_002486"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0759271.1"
                     /translation="AVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVGAE
                     YADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHEIV
                     NVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQVT
                     GLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHESYQ
                     LEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEGHI
                     DALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVTVQ
                     QVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVIDV
                     IHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNEGALAATV
                     SHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTAQS
                     LAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDVEKFAFTTLEVT
                     E"
     gene            complement(1749..3140)
                     /gene="uhpT"
                     /locus_tag="ACHUPB_002487"
     CDS             complement(1749..3140)
                     /gene="uhpT"
                     /locus_tag="ACHUPB_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0759272.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3278..4597)
                     /gene="uhpC"
                     /locus_tag="ACHUPB_002488"
     CDS             complement(3278..4597)
                     /gene="uhpC"
                     /locus_tag="ACHUPB_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0759273.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4607..6109)
                     /gene="uhpB"
                     /locus_tag="ACHUPB_002489"
     CDS             complement(4607..6109)
                     /gene="uhpB"
                     /locus_tag="ACHUPB_002489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0759274.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6109..6699)
                     /gene="uhpA"
                     /locus_tag="ACHUPB_002490"
     CDS             complement(6109..6699)
                     /gene="uhpA"
                     /locus_tag="ACHUPB_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0759275.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6774..7064)
                     /gene="ilvN"
                     /locus_tag="ACHUPB_002491"
     CDS             complement(6774..7064)
                     /gene="ilvN"
                     /locus_tag="ACHUPB_002491"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0759276.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7068..8756)
                     /gene="ilvB"
                     /locus_tag="ACHUPB_002492"
     CDS             complement(7068..8756)
                     /gene="ilvB"
                     /locus_tag="ACHUPB_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0759277.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8862..8960)
                     /gene="ivbL"
                     /locus_tag="ACHUPB_002493"
     CDS             complement(8862..8960)
                     /gene="ivbL"
                     /locus_tag="ACHUPB_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0759278.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9525..9614
                     /gene="tisB"
                     /locus_tag="ACHUPB_002494"
     CDS             9525..9614
                     /gene="tisB"
                     /locus_tag="ACHUPB_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0759279.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9738..9812)
                     /gene="ysdE"
                     /locus_tag="ACHUPB_002495"
     CDS             complement(9738..9812)
                     /gene="ysdE"
                     /locus_tag="ACHUPB_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0759280.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9894..11078
                     /gene="emrD"
                     /locus_tag="ACHUPB_002496"
     CDS             9894..11078
                     /gene="emrD"
                     /locus_tag="ACHUPB_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0759281.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11086..11583)
                     /locus_tag="ACHUPB_002497"
     CDS             complement(11086..11583)
                     /locus_tag="ACHUPB_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0759282.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11580..11942)
                     /locus_tag="ACHUPB_002498"
     CDS             complement(11580..11942)
                     /locus_tag="ACHUPB_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0759283.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11932..12279)
                     /locus_tag="ACHUPB_002499"
     CDS             complement(11932..12279)
                     /locus_tag="ACHUPB_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0759284.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12388..12837
                     /gene="yidI"
                     /locus_tag="ACHUPB_002500"
     CDS             12388..12837
                     /gene="yidI"
                     /locus_tag="ACHUPB_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0759285.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12884..13042)
                     /locus_tag="ACHUPB_002501"
                     /pseudo
     CDS             complement(12884..13042)
                     /locus_tag="ACHUPB_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13086..>13483)
                     /locus_tag="ACHUPB_002502"
     CDS             complement(13086..>13483)
                     /locus_tag="ACHUPB_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0759286.1"
                     /translation="SNMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQ
                     LWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3165 a         3775 c         3535 g         3008 t
ORIGIN      
        1 gccgtttccc gtggcgacgc tgttgccgat tatattattg ataatgtctc tattctcgac
       61 ctgatcaatg gcggagaaat ttccggccca attgtgatta aaggacgtta cattgccggt
      121 gttggcgcag aatacgctga tgctccggct ttgcagcgga ttgatgctcg cggcgcaacg
      181 gcggtgccag ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc
      241 acttttgaaa ccgctaccct gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa
      301 atcgtcaacg tgatgggaga agccggattc gcctggtttg cccgctgtgc cgaacaggca
      361 aggcaaaacc agtacttaca ggtcagctct tgcgtacccg ccctggaagg ctgcgatgtt
      421 aacggtgcca gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc
      481 ggccttgcag aaatgatgga ctaccctggc gtaattagcg ggcagaatgc gctgctcgat
      541 aaactggatg catttcgcca cctgacgctg gacggtcact gcccgggttt ggggggtaaa
      601 gaacttaacg cctatattac tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa
      661 gaaggacgcc ggaaattaca actcggcatg tcgttgatga tccgcgaagg gtccgctgcc
      721 cgcaatctca acgcgctggc accgttgatc aacgaattta acagcccgca atgcatgctc
      781 tgtaccgatg accgtaaccc gtgggagatc gcccatgaag gacacatcga tgccttaatt
      841 cgccgcctga tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg
      901 tcgacggcgc gccactttgg tctgaatcac ctcggcttac tggcacccgg caagcaggcc
      961 gatatcgtcc tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc
     1021 gagccgattg atgcgcaaac cttacaggcg gaagagtcgg cgagactggc acaatccgct
     1081 ccgccatatg gcaacaccat tgcccgccag ccagtttccg ccagcgactt tgccctgcaa
     1141 tttacgcccg gaaaacgcta tcgggtcatt gacgtcatcc ataacgaatt gattacgcac
     1201 tcccactcca gcgtctacag cgaaaatggt tttgatcgcg atgatgtgtg ctttattgcc
     1261 gtacttgagc gttacgggca acggctggct ccggcttgtg gtttgcttgg cggctttgga
     1321 ctgaatgaag gtgcgctggc tgcgacggtc agccatgaca gccataatat tgtggtgatc
     1381 ggtcgcagtg ccgaagagat ggcgctggcg gtcaatcagg tgattcagga tggcggcggg
     1441 ctgtgcgtgg tacgtaacgg ccaggtccaa agtcatctgc cgttgcccat tgccggcctg
     1501 atgagcaccg acacggcgca gtcgctggcg gaacaaattg acgccttgaa agccgccgcc
     1561 cgtgaatgcg gtccgttacc cgatgagccg tttattcaga tggcgtttct ttctctgcca
     1621 gtgatccccg cgctaaaact aaccagtcag gggctatttg atgtcgagaa gtttgccttc
     1681 actacgctgg aagtcacgga ataataaaaa agcccggcgt catgccgggc aaaagtcacc
     1741 agttacgttt atgccactgt caactgctga atttttttct cgcggcggat tttgcgttct
     1801 tccatcaccg ccactatcgc catcaggcag atacaaccaa tcgcggcgat atcaagtgcg
     1861 gcgaaggtgc ctgcccagcc ggtaaggccg aataccggcg tcccatcggc aatcattccc
     1921 agacctaact tggcaaagct gtcaccaatc aggtaagcaa aggtgccttt aataccatcg
     1981 gcagcgccaa tcgctttttt aggtacaaag ccaacagcag ccacaccaat caacaattgc
     2041 gggccaaaga ccaggaaacc caacgcaaag agagaagcca gatagatata ttcgttactg
     2101 gcatgttgat acacaccgag cgtggcgata atcagcgcca gcgcgatgca ggccaccagg
     2161 ccacggcgac cgttcgccag gtcagagagc cagccccaca gcagcgtacc gaccagcgca
     2221 ccagcttcaa acagcgtaaa gccctgaatc gccaccgctt tagagagttt cagttcctgg
     2281 aacgcgtata cggttgacca ctggtcgata ccaatacgta ccacatagag gaaaatgttg
     2341 gcgaagcaca gcagccagat cactttgttt ttcagcacat actcaacaaa gatctgccac
     2401 ttggtcatat cggtagattc tgtctctttg tcctcttcgc tgatctcctc gccgaacagt
     2461 tcttcagctt tgccgaggcc ataagattcc ggggagtcgc tgccgtaacg caggccgata
     2521 aaaccgacaa tcagcgcgat aatcgacggg aagataaaca tgccgatgac atggccatcg
     2581 aacaggtaat ttgccccgaa cagcgccacg cctgctgcgc ctgcaccgcc aaggttgtga
     2641 gaaatattcc agaaaccgag gaatgtcccg cgtttacgac gcggcgtcca tttggtgatg
     2701 gtggagtaac tgcacgaacc gccggtactc tggaaaaagc cgcttaaggc gtagaaggca
     2761 atcatcagga acaggctaac cgagccgctg cccatactgg cactgaagcc cagcatacaa
     2821 atggcagaca ggatcagcat gaacggcagg aattgtttgg tgtttttgcc gtcggcgtag
     2881 taggaaacca gcgttttacc cacgccataa gtgatggaga aacccaggcc gatcatcccc
     2941 agctgcgtca tgctcaaccc gtaggtcgag atcatgtcgt tctgcgcgat gttaaagttc
     3001 ttgcgaatca ggtacatcgt caggtagccg ataaagacca ccaggtagga ttgcatgaac
     3061 ggtttgaacc acattttgcg ccgcacttcg agcggaaggt ccagggtcgg cttgcgaacc
     3121 tggtttaaga aagccagcat gggttactcc tgaaatgaat acctgcgtga ggcatgcatt
     3181 gtaaaaatca gcagcaaatc aggcctgaga tagcgtccag gcaaaacctg gaaaatttct
     3241 tagttttgcc ggatatgaag tgaaaaggtg agatgcatca cgcttcgcgc ggtgtctggg
     3301 cgttcaaaaa gggcagtaac agcagtgcgg aaatcccggc ggcgatagag ataaccacaa
     3361 aaaatccgct ccagtgccag gtatcgagta ctttcgccag cggccaacca gcaagcgacg
     3421 cccccagata agcaaacaag ccgacaaacc ccgtcgccgc ccctgccgcc tctttgtggg
     3481 aacactctgc cgccgccata ccgattaaca tctgtgggcc aaagacaaaa aaaccaatgg
     3541 tgaagaagca ggttgcctgc atcacgtagc tggcaaatgg catcagccac agggagccga
     3601 ctgaaagcaa aattccggcg gcgaaaatca aattcatcgg ccctcggttg ccgttaaaca
     3661 atttgtccga gccccaaccc gctaccagcg caccgataaa tccacccagt tcaaacatcg
     3721 tcactgccgt attcgccgtg accagatcga cgcccagcgt ctcggacata tacaaattgc
     3781 cccagtcgtt gatcgccgcc cggaccacat agaccagcac atagcaaaac gaaagcagcc
     3841 agatatacgg attcagcaac acatatttgg tgaggatctc tttacgcgtc agccctgccc
     3901 cttcttgttg ttgagctatc tccagcgcgt cgtgtcgcca ttcaccgacc gccggtaaac
     3961 ctaacgcctg cgggcgatcg cgcagccgcc agcagagaaa aattcccacg acgatcgcca
     4021 tacaaccagc aatcatcatc ccggcacgcc agccgtaatg tagtgcagcc gctgccatca
     4081 caatgggaat gagtgcgccg ccgacgttat gcgccgtgtt ccataatgcc caccaaccac
     4141 cgcgctcggt acgtgaatac caggccgtta acagacgcgc acacaccggt gaaccccagc
     4201 cctggaaaaa ggcgttcagc acccagagta cggcaaacgc ccatagcgac gtcgagaagc
     4261 caaacagaat gttgataatg cccgtggcga taagccctat ccccataaaa taacgggcat
     4321 ttgagcgatc gctgacaatg ccggagacaa acttcgacac gccataggta atgtaaaaca
     4381 gggtcgctaa caggccgata tcgctacggc tgagcacgcc gttagcaagg atttctggta
     4441 cggcggcgtt aaaacttttc cgcgtgaagt aaaacagcgc gtaacccagc cagatggtca
     4501 gcagaatatg ccgacgccag tagcgatagc gggcatcaat ttcatattta tcagccatta
     4561 atggcgcatc ggcaggcgct ttcagaaacg gcaacatcat caaaccttag acatagcgtt
     4621 gaggtagaga aacgctgaca cgcgtgccgt gcagacagga aatgtgtaat gtgccaccca
     4681 gcgccgttac gcgctcgcgc attccggtga ggccaaaacc ttgttgcccg gaacccggcg
     4741 gcaaaccgct gccatcgtct tcaataacca gcatcaaccg ttcatcctgc tgccagcctt
     4801 gcagggtgac cgcgctggca tcagcatgtt tcacaatgtt gttcagccct tcctggcaga
     4861 cacgaaacag cgtcacgcgc tggttttcgc ttaacgctga ttcatcgatt cgccattcga
     4921 gatggctgac aataccgcgc ccttccagct ccatttcccg catcagtgag cggatggcct
     4981 gctccagggt gagatcatcc aactggcgcg gacgtaaccg acccaacaaa cggcgcaccg
     5041 cgtcgtaaac gcccagcgat agttgttcga tgagttgccc gctctgcttc acgctggcgt
     5101 tatctgccgc cagccgctga acaatgcccg cctgagtacg aatagcagtg atggtctggc
     5161 cgatatcatc atgcagctca cgcgccacat cacggcgcac gctctcttcg gtttccagca
     5221 accgttcagc cagatgctga ttgcgcgcca gttccttttg cagcgactgg ttaagttcac
     5281 gcaaccgctg gatgccagcg ccaagcaaca accctgtcag actttgcacc agcagcgaga
     5341 gcaataaatc caccggatga tcgcgccagg tttgactggc gatcagcgcg atggcgttca
     5401 tcaacgtcgc aatcagcgcc ccttgccaac catagtgcca ggccagcgcg ataatcggca
     5461 gcaccagaca gaatggcgta aagcgcgaca gttcgtccgg caatcccaac tggagccaga
     5521 gactgataac aaacagcagc aagtaccaga ccagatgtcg cccgcgccag ttgattggct
     5581 gagaaaccag tgacgggccg agcggcagcc aggtgttatt ggcgagatag tgccagaaca
     5641 ccagacatat cggggccagc gtcaggccgc cagttaaagt cagcaacagc gcattccacg
     5701 actctttgcc gtgccaaagc cagggcagcg actgcaacaa cgccgccgcc gttaacgccg
     5761 ccccctgtaa cagcaaggtg cgccagtcac gctgatggcg atagcgcgag atcagcgcta
     5821 cgggcagtaa cgtcagtaaa ctaccgatca tcaataacgg aaaatgggtt aaaccgaccg
     5881 cctgcgttag ccagtaaatc agcagccact ccgcacccag caatacgggc cagtatccgc
     5941 gcgggcattg cagcattagc cccagacgca gaccaaacgg aaataacagc accgccatat
     6001 cagggcgctc aaccagatgc aggctgatac tccacaggca aaaccatgcg gcagagaaga
     6061 taaaaaagca ggcaataacg gtaattaagc gggagaacaa cgtcttcatc accagccatc
     6121 aaacatgcgg cgcgccagct ctacgtcgtt actgacgccc agtttttcca tcagattggc
     6181 gcgatggacg tgtaccgttt tcggtgacaa gcccagttcg gcggcaatct ctttcaccgc
     6241 cattccttgc gccagttttt ccgccacctg gcgttcacgt ttggttagcg ggtcctgacg
     6301 accggatgcc agtttaatgg caatatccgg cgtcagataa cagccacccg tggcaaccgt
     6361 atgcaccgca gcaatgagtt catccgggct acagcgtttg gaaagaaagc cgcgtgcccc
     6421 cgcgttaagc gcctgctcaa ccagcgcagg actgtcgtga acggagagca taatcgtcgc
     6481 catacctttc ggcagctggc ttagcagctc cagaccggag atatcgggca tggagatatc
     6541 gcaaatacac acctgcacac cgcgccccgg cagccccgcc agcgcctcgc gccccgaacc
     6601 aaactcggca actacctgca aatcaggttc cagccccagc agctgcgcaa agccggagcg
     6661 gacgatgagg tgatcgtcta taagggcaac ggtgatcatg gtcttgtcct ggcgggtaaa
     6721 aaaaaacgcg cttaccttaa cgataagcgc gatgttgttc aagccttgag cggttactga
     6781 aaaaacaccg cgatcttgtt aaacatcgtc ggatcggact gattacgctg cactttcacg
     6841 acatcttcca gcttatcgat ttggcttatc atctgctcca gacgctggtc gtcattgacc
     6901 agtagccaga tatggctttt gtcgctgtcc tgaatcggca gacaaagaat gccttcaacg
     6961 ttaaaagcgc ggcgggcaaa aaggccacaa acgtgggtca ttacgcccgg atggttgcga
     7021 acggtgagct ccagaattac gttgtcatga gttgtgtttt gcatggctta ttcccccacc
     7081 atttcagtat tcgccgcacc tggcggcacc atcgggtaaa ctttttcttc ggcatcaatg
     7141 cgcacatgga tcagcgccgg gctagggcga ttgatgattt cctgcaatgc agcctgcgga
     7201 tcggcttcgt tattcaaatc acaggtttcg aggccgaatc cggcggcaat ctgcataaag
     7261 ttgattttgc ccggataggt ggcggcaaaa acgccttgct cgtagaacag actctgttgc
     7321 tgatgcacca gccccagcgc ttcgttgttc atcagaatga ttttgacatc cagctgattt
     7381 tcactggcgg tcgccatctc ctgaatattc atcatcaggc tgccgtcgcc ggagaaacac
     7441 aacactttgc gatccgggtt cgccagcgcc gcgccaatcg ccgcaggcag gccaaaaccc
     7501 atcgtgccca gcccaccgga ggtcagccac tggcgtgggc gattgagcgg ataagcttgc
     7561 gcggtccaca tctgatgctg acccacatcg gtggtgataa tcgcattgtc atcgacacag
     7621 gcggcaacgg cgttgatcag gccgtaatgg cttaatggat cgcacgcttt cgggattgga
     7681 cacgggaatt cgcgctgcaa atccgctacc aactggtgcc actctgcacg cggttgcgct
     7741 tccacctgcg ggatcaattg cgccagcacg tcatcaacat ccgcctgaat cgccacatgc
     7801 ggctgcttga ttttacccag ctctgcgcga tcgatatcga catgaataat tttggcattc
     7861 ggacagaact gctcggtttt gccaatcgcc cggtcatcaa aacgcgcacc gagcacaatc
     7921 aacaaatccg cctcctgcaa aatatagttg gtactgcgca cgccgtgcat ccccagcata
     7981 cccagcgaca acggatgcgc ttttggcagc atgcccagcg ccattaaagt catggtggta
     8041 ggcagttgcg ctttctccgc cagttcacgc acccgcgcag gcgcattgat cacaccacca
     8101 cccagataaa gcaccgggcg tttggcagcg ttaatcattg tagctacgtc acgaatgctt
     8161 tcttcgctaa aggcgggtgc agcggctttt tctgccaccg cgggctgagc ttcaatctca
     8221 aaaaccgccg tttgtacatc cttaggaatg tctatccaca ccgggccagg gcggcctgat
     8281 tgcgcaatgc ggaaggcatc gctcatgacc tgcgggagtt cttcgatatg tctgaccaga
     8341 tagttgtgtt tggtgatggg gatagagatg ccgtaggtgt cgacttcctg gaaggcgtcg
     8401 gtgccgatca tcgaggcggg aacctgacca gtgatgcaaa tcagcgggat ggagtccagc
     8461 cgcgcatcgg caatggcggt caccaggtta gtcgcacccg gtccgctaca ggccatacag
     8521 accgccggtt taccgtcggt gcgcgccatt ccctgagcga taaagcccgc gccctgttca
     8581 tgacgggcca gaatatggcg gatttgcgtg ctttggctta aggcatcgta aacaggcagg
     8641 atagaaccgc ccggaatgcc tgtcacaatc ttaatgccct gctgttccag gaaatgaacg
     8701 ataaattctg cgccggtaaa gcgcttacgc gtcaatgttg tgcccgaact tgccatgctc
     8761 cagtcctttt cttctgggcc gactttccgg gaggtgctta aacgaaaaac cccgcccggt
     8821 ttgcgccgac ggggttttgg aatcgtgtgt tgttccagtc cctacggcgc attgccgacg
     8881 accaccacca cacgcacgac gaccactgcg gcggatggcg cagttggtag tagttttgcg
     8941 ttgaacatgg aagtagtcat tggggacctt gttggttttg tgtttaacaa tatttataca
     9001 agcacagctt tacaggggag acaatggaaa atttttcagc aagggaaaat tgaggggttg
     9061 atcacgtttt gtactgaatt gcagataaca aaaaaccccg ccggagcgag gtttcgtcag
     9121 tcgcccgcgg ctggtaaccg caaagcacac tgtattatgt caacacagaa agtatacgtg
     9181 ttccgcgcag aacgcgcaat ttcggcacga attttgacgt atttagtgca tagttgagta
     9241 tcgatcacag tttgcgtttt gtccaaatat tgctgtttat ttatacagta aacttctata
     9301 atatcactgt acgcaatgtg ttatgcgggg gccgcatcgt tacccggcgc actaagtcct
     9361 ggctgaaacg ggtggtgccg tcagcgcctt aaccccgcgt gagcacactg tgttatgtca
     9421 acaagcacaa agtttctcct tgagataccg cgtgcacaac agctggcaac aggcagcgga
     9481 aaggtacgtc agctggcagt gctcctgaac cacaggagac gcgtatgagc ctggtggata
     9541 tcgccattct tatcctcaaa ctcattgttg cagcactgca actgcttgat gctgttctga
     9601 aatacctgaa gtaattcaga ttcaagtcgc accaaagggg agcgggtaac cgctcccctt
     9661 ttatatttag cgtgcgggtt ggtgtcggat gcgatgctga cgtatcttat ccgccctacc
     9721 atctctcccg gcaacattta ttgccgcttt tgtttacata ttctgccgct aaacaactcc
     9781 ccattcctgg cgtatatctg gctaacattc atcaatgtga tagattcctc tcccgcattt
     9841 atgggaatgc gtagtgactt actctaatta tttttataaa agcatccgtg ataatgaaaa
     9901 ggcatagaaa cgtcaatttg ttattgatgt tggtattact cgtggccgtc ggtcagatgg
     9961 cgcaaaccat ttatattcca gctattgccg atatggcgcg cgatctcaac gtccgtgaag
    10021 gggcggtgca gagcgtaatg ggcgcttatc tgctgactta cggtgtctca cagctgtttt
    10081 atggcccgat ttccgaccgt gtgggtcgcc gaccggtgat cctcgtcgga atgtccattt
    10141 ttatgctggc aacgctggtc gcggtcacga cctccagttt gacggtattg attgccgcca
    10201 gcgcgatgca ggggatgggc accggcgttg gcggcgtaat ggcgcgtact ttgccgcgag
    10261 atttatatga acggacacag ttgcgccatg ctaacagcct gttaaacatg gggattctcg
    10321 tcagtccgtt gctcgcaccg ctaatcggcg gtctgctgga tacgatgtgg aactggcgcg
    10381 cctgttatct ctttttgttg gttctttgtg ctggtgtgac cttcagtatg gcccgctgga
    10441 tgccggaaac gcgtccggtc gatgcaccgc gcacgcgcct gcttaccagt tataaaacgc
    10501 ttttcggtaa cagcggtttt aactgttatt tgctgatgct gattggcggt ctggccggga
    10561 ttgccgcctt tgaagcctgc tccggcgtgc tgatgggcgc ggtgttaggg ctgagcagta
    10621 tgacggtcag tattttgttt attctgccga ttccagcagc gttttttggc gcatggtttg
    10681 ccggacgtcc caataaacgc ttctccacgt taatgtggca gtcggttatc tgctgcctgc
    10741 tggctggctt gctgatgtgg atccccgact ggtttggcgt gatgaatgtc tggacgctgc
    10801 tcgttcccgc cgcgctgttc tttttcggtg ccgggatgct gtttcctctg gcgaccagcg
    10861 gcgcgatgga gccgttcccc ttcctggcgg gcacggctgg cgcgctggtc ggcggtctgc
    10921 aaaacattgg ttccggcgtg ctggcgtcgc tctctgcgat gttgccgcaa accggtcagg
    10981 gcagcctagg gttgttgatg accttaatgg gattgttgat cgtgctgtgc tggctaccgc
    11041 tggcgacgcg gatgtcgcat caggggcagc ccgtttaagc gcacgtcacc gcagcatcgt
    11101 catcagctcc atgggagaac gatgctgctt tatcagatca cgcatcaccc gcatatgcgg
    11161 tgcggagtaa gaataaaacg cctgatagcc cgcacaaagc acgctgtctg gcgtctcctg
    11221 ccgagcgtgc gggcacaggc gatgccagtc gcacgcctgg cagtcacagc gcggttgcgg
    11281 gttttcgctg ccacaccatt tttgtaaggt ggcgtcaaaa agctcaatcg agatacgact
    11341 gatatctgcc cgcacccaac actcgaacag ggcaattaaa aatttccccc acagctctgc
    11401 tgtcaccgaa gcggccgtca gatcgccccg gtcatcaaat tcaagatagg gaatgagctg
    11461 tacggagcgg atgccccgct gtttgaaatt gtgataaaaa tcggcaggtt gcagagcatc
    11521 cttgtactct acaaccagtt tatgcctgtc ctcttcagca tcaatgacct gacttcctgt
    11581 catgcgactc tctcaataaa tacgataagt tgatgtatat gcgttacagc aaacagtatt
    11641 gcgagagata acaccgcgag ggagatcaaa aatttgtcac ggactacgtg aaattgggaa
    11701 aaatcgctat tcgtgacatc cattaaattg cgattacggg tgtagtgcca gaggatcagc
    11761 gccacgatgg cgagaatgcc aatcgaaatc cagaataaca tgcccgcctg gtgccagttg
    11821 tgtttgatag ccaacgccat caacgcgccg tagcccagca tggtacgaaa ccacgccagt
    11881 gatgtgcgct ccggctgcag tcctgggtcg gcaatgcgtc tggctttgcg gctatccggc
    11941 atacaacacc agtcccataa cgatcaccgc cacgaccatc aatattaagc taatgattaa
    12001 taagctgttg gtgtagggca ggtcctcttt cagacgcatc gctttttcat tgcgtaacca
    12061 gcgcaggtag ccatacattg ctaacccgcc ggaaaacaga cacaacaaca acgccagcag
    12121 ttcacgaatg acgggcgtgg cgaaatctgg cgcaagttga tcaagcccaa cccccgccgc
    12181 cagaaatcct agtgcggtac ggatccacgc cagaaaagta cgctcattag ccagcgagaa
    12241 gcggtaatcg ggcgcttctc caaggcggga aattttcatt gttgctcctt tgcaatttca
    12301 ctaactctct acctttagcg gtttttttta agaatttcgt ccgcccgttt cctgatatgc
    12361 ttttgccatc cgctacaagg aggtcagatg ggtatcatag cgcaaaataa aatcagttca
    12421 ttgggcatgc tgttcggtgc catcgcttta atgatgggga ttatccactt ttcattcggc
    12481 cctttttccg caccaccgcc aacgcttgaa agcatcgtgg cggacaaaac ggctgaaatt
    12541 aaaagaggtc ttctcgccgg aataaaaggc gaaaaaatta ccaccgtgga gaaaaaagag
    12601 gacgtagatg tcgataaaat cctcgatcaa agcggtatcg cactggccat tgctgcgcta
    12661 ctttgcgcat ttgttggcgg tatgcgtaag gaaaatcgct ggggcattcg cggcgcgctg
    12721 gtttttggcg gcggaacgct ggctttccat accttgcttt ttggtatcgg catcgtgtgc
    12781 agcattttgc tgatattcct tatcttttcc ttcctgaccg gcggttcgct ggtttaagca
    12841 gcctgaataa tgcccgccgg gtgatcatca gcgggcatta tcgtcagaac ttctgttttt
    12901 tctcttccac cttcacccct tgtgtcggta ggccggtgtc atagtcacgt accaccggcg
    12961 aatagccatc ttgtgggcgt ggacgaaatg cccccatcca gcgcggccgt gcatctttac
    13021 gccacggacg caggctccat tgttgacatc ctccacgccc tgaaggacgt ggacccctgc
    13081 tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct gcttcaacga gcggcctgac
    13141 tgcaccatct ctccacaggc aagaacggcg tgccccgccg ctaaaatgtt acgagcgcca
    13201 tttacatcgg cgttcgctgt atatccacat acctggcatc tgaatttact ttgtgacagg
    13261 cgattttctt tcgctgtatg accacagcac acgcaacgct ggcttgtgta cgctggcgga
    13321 acagcaagca cctgaccacc acgccagagc tgcttgtacg caagctggcg gcgcatttca
    13381 taccagccct gatccagtat cgaacggttt aaacctgatt ttgcccggac attgcgcccc
    13441 ggcaggctga ccgtacccgc tgctgacttt gacatgttgc tga
//
LOCUS       DAZOZD010000106        13423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOZD010000106 DAZOZD010000000
VERSION     DAZOZD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.106"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            537..1052
                     /locus_tag="ACHUPB_002503"
     CDS             537..1052
                     /locus_tag="ACHUPB_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0759287.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1270..1998
                     /gene="artP"
                     /locus_tag="ACHUPB_002504"
     CDS             1270..1998
                     /gene="artP"
                     /locus_tag="ACHUPB_002504"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0759288.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            2016..2747
                     /gene="artI"
                     /locus_tag="ACHUPB_002505"
     CDS             2016..2747
                     /gene="artI"
                     /locus_tag="ACHUPB_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0759289.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2754..3470
                     /gene="artQ"
                     /locus_tag="ACHUPB_002506"
     CDS             2754..3470
                     /gene="artQ"
                     /locus_tag="ACHUPB_002506"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0759290.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3470..4138
                     /gene="artM"
                     /locus_tag="ACHUPB_002507"
     CDS             3470..4138
                     /gene="artM"
                     /locus_tag="ACHUPB_002507"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0759291.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4430..5161
                     /gene="artJ"
                     /locus_tag="ACHUPB_002508"
     CDS             4430..5161
                     /gene="artJ"
                     /locus_tag="ACHUPB_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0759292.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5336..6463)
                     /gene="rlmC"
                     /locus_tag="ACHUPB_002509"
     CDS             complement(5336..6463)
                     /gene="rlmC"
                     /locus_tag="ACHUPB_002509"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0759293.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6504..6992)
                     /locus_tag="ACHUPB_002510"
     CDS             complement(6504..6992)
                     /locus_tag="ACHUPB_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0759294.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(7052..7897)
                     /gene="potI"
                     /locus_tag="ACHUPB_002511"
     CDS             complement(7052..7897)
                     /gene="potI"
                     /locus_tag="ACHUPB_002511"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0759295.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7894..8847)
                     /gene="potH"
                     /locus_tag="ACHUPB_002512"
     CDS             complement(7894..8847)
                     /gene="potH"
                     /locus_tag="ACHUPB_002512"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0759296.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8857..9990)
                     /gene="potG"
                     /locus_tag="ACHUPB_002513"
     CDS             complement(8857..9990)
                     /gene="potG"
                     /locus_tag="ACHUPB_002513"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0759297.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10085..11197)
                     /gene="potF"
                     /locus_tag="ACHUPB_002514"
     CDS             complement(10085..11197)
                     /gene="potF"
                     /locus_tag="ACHUPB_002514"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0759298.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11548..12024)
                     /locus_tag="ACHUPB_002515"
     CDS             complement(11548..12024)
                     /locus_tag="ACHUPB_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0759299.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12112..13014)
                     /gene="rimK"
                     /locus_tag="ACHUPB_002516"
     CDS             complement(12112..13014)
                     /gene="rimK"
                     /locus_tag="ACHUPB_002516"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0759300.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13075..>13423)
                     /gene="nfsA"
                     /locus_tag="ACHUPB_002517"
     CDS             complement(13075..>13423)
                     /gene="nfsA"
                     /locus_tag="ACHUPB_002517"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0759301.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         3410 a         3562 c         3284 g         3167 t
ORIGIN      
        1 aatttattct tcgctaaaat tcattagctt aaattcttta gccacaattg tagcataaat
       61 aatattatcg agagtcaaag ctgggactat caaatttcgc tcattttcaa aaatacaacg
      121 taattttggc aatcatctta atcgcaggat ctcttatcgc atgtgaaaaa ttttgtaatg
      181 attagacaat aaccgcgttc atttatcacg cgttataaat gtgaaactgg cttcttgcag
      241 atgctcttaa cctctacagg aggatttata ccaccatttc agaactatca tatttacctt
      301 aaatgatcat ttcagccata taacacgctc aacctcacaa atggtttaca ctgtctattg
      361 tttgggtaat ttaagtaagt cagctacgag caaccttgag tggatagcca gcaatggact
      421 caaacccaat caggtgttca gattgtttca gaattgtatt aaaaacaatg tattaaataa
      481 tctgatagcg tttggtcgta gtgtgacgca gacagcaagc taataaggaa atgattatgc
      541 gctaccgttc actatctcta cttattccat gtgcactgct gctgagtgcc tgtaccaccg
      601 tcacgccagc ttataaagat aacggtcccc gcaccggttc ctgtgtccag ggggggcctg
      661 acagcgtggc tcaacaattc tatgactacc gcattcaaca tcgcagcaat gacattaccg
      721 ccctgcgccc ctatttgagt gacaaactgg caacccttct tagcgatgcc agccgcgata
      781 atagtcatcg tgaattgtta agcagcgatc cgttctccag tcgaaccacc ttgccagata
      841 gcgcccacgt tgccagtgca tcgactatcc ctaatcgtga tgcccgtaac attccattgc
      901 gcgtagatct caaacagggt gaccagggtt ggcaggatga agtgttaatg attcaggaag
      961 gccagtgttg ggtcatcgac gatgtgcgct acttaggcgg cagcgttcat gccacggcgg
     1021 gtacgctccg tcagtccatc gagaatcgct aacgccttga tcaaccaata aaccccgtaa
     1081 attgtccggc atttcgtgag gaatgccgac atttatgctc gccgaccacc gcccccgtta
     1141 ttttgtgcta tgtttattga ataatgcgct ttgcttttaa cttttaaagc agaaatattg
     1201 cataattatt ctgtcaaagg tactatcttc gacctcaata gctatcagac tgccagtata
     1261 cgagtgtcaa tgagtattca attaaacggc attaattgct tctacggcgc gcatcaggcg
     1321 ctgttcgata tcacgctgga ttgcccacag ggcgaaacgc tggtgttact tggccccagc
     1381 ggcgcgggta aaagctcgct gctgcgtgta ctcaatctgc ttgagatgcc acgctccggt
     1441 acgctcaaca ttgcaggcaa ccatttcgat ttcaccaaaa caccctctga caaagcgatt
     1501 cgcgatttgc gtcgtaacgt tggcatggtg tttcagcaat acaacctgtg gccgcatctg
     1561 accgtgcagc aaaacctgat tgaagcgccc tgccgtgtac tggggttgag taaagatcag
     1621 gcgctggccc gtgcagaaaa actgctggaa cgtctgcgtc tcaaacctta tagcgatcgt
     1681 tacccgctgc atctttctgg tggtcagcag cagcgtgttg ctattgcccg tgcgttgatg
     1741 atggaaccgc aggtactgct gttcgatgaa ccgaccgccg cactggaccc ggaaattacg
     1801 gcacaaatcg tcagcatcat tcgtgagctg gcagaaacga atattaccca ggtgatcgtc
     1861 acccacgaag ttgaagtggc gcgtaaaacc gccagccgag tggtgtatat ggaaaatggt
     1921 catatcgtag aacaaggcga cgcgagctgc tttaccgagc cgcaaaccga agcatttaaa
     1981 aactatctct ctcactaaga ttcgggataa caacaatgaa aaaagttctg attgccgcgt
     2041 taattgcagg ttttagtctt tccgccacag ctgccgaaac cattcgtttt gctaccgaag
     2101 catcctatcc tccgtttgaa tcgattgatg caaacaacca gatcgttggt tttgacgtcg
     2161 acctggcaca agcgctgtgt aaagagattg atgcaacctg cactttctct aaccaggcgt
     2221 ttgacagcct gatcccaagc ctgaaattcc gtcgcgtaga agccgtgatg gcgggcatgg
     2281 atatcactcc ggagcgtgaa aagcaggtgc tgtttaccac cccgtactat gacaactctg
     2341 ccctgtttgt gggtcagcaa ggcaaataca ccagtgttga tcagctgaaa ggcaaaaaag
     2401 tcggcgtaca gaacgggacg acacaccaga aattcattat ggataagcac ccggaaatca
     2461 ctaccgttcc gtatgacagc taccagaacg caaaactgga tctgcaaaac gggcgtatcg
     2521 acggcgtctt cggtgacacc gcagtggtca ctgagtggct gaaagataac ccgaaactgg
     2581 cggcggtggg cgacaaagtg accgataaag attacttcgg cactggcctc ggcatcgcgg
     2641 tacgtcaggg caacactgag ctgcagcaga aactcaacac tgcgctggaa aaagtgaaga
     2701 aagatggcac ttacgaaacc atctacaaca aatggttcca gaagtaattc ctgatgaatg
     2761 aattttttcc tttagcaagc gccgccggga tgaccgtcgg ccttgccgtt tgtgcattga
     2821 ttgtcgggct ggcgctggcg atgttctttg cggtatggga gtcggcaaaa tggcgtcctg
     2881 tcgcgtgggc aggttcagcg ctggtaacca ttctgcgtgg cctgccagaa attctggtgg
     2941 tgctgtttat ctattttggc tcctcgcagc tgctgctgac gctttcggat ggcttcacta
     3001 tcaatcttgg gttcgtgcag atcccagtgc agatggacat tgagaacttc gacgttagcc
     3061 cgttcctttg tggtgtcatc gctctgtcac tgctgtatgc cgcctatgcc tcgcaaacgc
     3121 ttcggggcgc gttgaaagcg gtgccggtgg gtcagtggga gtctggtcag gcgctggggc
     3181 tgtcgaaatc ggctatcttt ttccgtctgg tgatgccgca gatgtggcgt catgcgctgc
     3241 ctggcctcgg taaccagtgg ctggtgctgc tgaaagatac cgcgctggtc agtttgatta
     3301 gtgtgaatga tttaatgctg caaacaaaaa gcatcgctac tcgtacccag gaaccattta
     3361 cctggtacat tgtggcggcg gcgatttacc tggtgatcac cctgctcagt cagtacattc
     3421 tcaaacgcat tgacctgcgc gcgacacgtt ttgagcggag gcccagctaa tgtttgagta
     3481 tttacccgaa ctgatgaaag ggctacacac cagcctgacg ctaaccgttg cctcgctgat
     3541 tgtggcactg attctggcat tgatttttac catcatcctg acgctgaaaa cgccggtgct
     3601 ggtgtggctg gtgcggggtt atatcacgct gtttaccggt acgccgctgc tggtgcagat
     3661 cttcctgatt tattacgggc cgggccagtt tccgacgttg caggagtatc cggcactgtg
     3721 gcatttgttg tcagaaccgt ggttatgtgc gctgattgcg ttatcgctga acagtgcggc
     3781 gtataccacg cagctgtttt acggtgcaat tcgtgcgatc ccagaaggtc agtggcagtc
     3841 ctgtagcgcc ctgggaatga gcaaaaaaga tacgctggcg ctcctgctgc cgtatgcctt
     3901 taaacgctcg ctctcttctt attccaacga agtggtgctg gtgttcaaaa gtacctctct
     3961 ggcttacacc attacgctga tggaagtgat gggatacagc cagttgttgt acggacgcac
     4021 ctacgatgta atggtgttcg gtgcggcagg gattatttac ctggtcgtca acggcctgct
     4081 gacgctgatg atgcgtctga tcgagcgcaa agcactggca ttcgaacggc ggaattaatg
     4141 tagctgcgta cattcgccgg gggggtttgt aggtctgata agacgcgcca gcgtcgcatc
     4201 agacatcagc acggtgcctg tgccggatgc ggcgtggacg ccttatccgg cctacatagt
     4261 gcataaattc ttataatgaa gacggacaac tcactaagtt atccgtcttt ttttatttca
     4321 tttaaattat ttaatcatat ttattgcata taaattcact tgatggcatt gttatcccat
     4381 gccgcagaca cggccaaaaa tcataagatt gacagacggg agttccatca tgaaaaagtt
     4441 agttcttgcc gctttacttg cttcctttac tttcggtgct tctgccgcag agaaaatcaa
     4501 ttttggcgtt tcagccacct atccaccctt tgaatctata ggtgctaata atgagattgt
     4561 cggctttgat atcgatctgg caaaagcctt gtgcaaacaa atgcaggcag aatgtacttt
     4621 tactaatcac gcgttcgaca gcctgatccc gtcactgaaa ttcagaaaat atgactccgt
     4681 aatctccggt atggatatca cgcccgagcg tagcaagcag gtatcgttta ccacgcccta
     4741 ctatgaaaac tcagccgtcg tgattgccaa aaaagatacc tacaaaacgt ttgccgatct
     4801 gaaaggcaaa cgtattggga tggaaaacgg caccacgcac cagaaatata ttcaggatca
     4861 gcaccccgaa gtgaaaactg tctcctatga cagttatcag aatgccttta tcgatctgaa
     4921 aaatggtcgt attgatgggg tatttggtga cacagcggtg gtaaacgaat ggctgaaaac
     4981 caatccgcaa ctgggcgttg ctactgaaaa agtgaccgat ccgcaatact tcggcaccgg
     5041 tctgggcatc gctgtacgtc cggataacaa agctctgctg gaaaaactga ataacgcgct
     5101 ggcagcaatt aaagctgacg gtacatatca gaaaatcaat gaccagtggt tcccgcagta
     5161 attgagactg atgacaaaag taagattgcc tgatgcgctg cgcttatctg gcccacatca
     5221 actctgcaat gtattgattt agcatgattt tgtaggccgg ataaggcgta cacgccgcat
     5281 ccggcatgaa caaagcgcac ctgatcagca atctgaagcc gcacctgcgg cttttttatt
     5341 gcttcaccag cagcgtcatc acttcatagt gcgcggtgtg cgggaacata tcaaaaagct
     5401 gtacccgttc gatgcggtaa cctggcagtt cgcggatatc tttcgccatg gtttgggcgt
     5461 tacagctgga gtagataata aaacgcggtg ccatcgttga gagataatca cacagcggtt
     5521 taccaatgcc gcggcgcggc gggttaacca gcaccagctc cggcacatcc ccctgagcgg
     5581 tggcaaactg agtggagtcc agcgcctgaa attgcaaacg cgttaagccc agttcagcgg
     5641 ctgactgctt tgcacaggca atggcctctg atgcaatttc gatcccggtt aactgcatgt
     5701 caggcgtcgc gcagtgtaaa ccaaagccac ccacaccgca gaacagatcc cacatatgtt
     5761 taactggcag ctgccgtacc cagtcgcgcg cggtagcgta cagctggctg gcgaccgccg
     5821 gattagtctg gaagaaactt tgcggacgga tccacagcgg tacgtcatta aaacgctctg
     5881 ccagcgcctg ttgttcagtc aggtagatct ccgtctcccc ttccataatc gccatatgta
     5941 ccggctgaat attgacggta ataactttca gttgcggtag ttgttcctgt aaccacggca
     6001 gcgccttacg cagttgtgcc agtttggttt cagaacgcag tacaaagcgc agcatcatgc
     6061 cgccatcgct ctggctttca gtcagcagaa tgtatttcag ttcgccacgc ttacgcgcca
     6121 cgttgtaggg cgttaacccc gcacgggcga taaacggttt tagcgccgca aaaacgggcg
     6181 caaaagaggc aggataaagc gggcagtcac aaaggtcttc cggcgtgcca tcgcgatgca
     6241 gcataccgag cagtggtttt tcaacgctac cactcaccac cattttggct ttattacgaa
     6301 acccttgttc cgggcctgac accggcgcgc accattcctc aaccggaaaa tcggcgagca
     6361 gatttttaag atcggcggtt ttagcggaga gttgctctgg aatcggctgc gttatccact
     6421 gacaggaacg acagcgaccc gcgtcgtaaa gtgcgcactg catacattga ccttcacatc
     6481 atcaggggcg acgattatac acattattgc aactggaaga accgccgact ggtcgaggga
     6541 acgaacagca gcatcaggat gagcatatcc ggcagcttct gcagcatcag gctatggaag
     6601 atttcacgtt ttgattcacc gggaatgctg aacagctccg gataaccgta ccccagcgaa
     6661 gccgcccaca agtaacttgc ggcggtgatt tgtgtcagca gatatagcca gcgcgcccag
     6721 cgacgccctt tcaccagtga aaacgcacac cagatctcaa tgaaaaccag caccagactg
     6781 cttaaaaaga ccagcgttaa actccaggtt tgtacgctgc gatgaatgaa ctcgccaata
     6841 ccgcgcacgc ccagcgtatt gaaaatcatc agcacgtcga ggccacggat cataataatg
     6901 gcgagcgccg ccacctgcac cagcgcaggc acattcaggc gagcatgaga tgaagatgtt
     6961 ttcttaaaaa atcccaacgt ttcgtcttcc atgaaaacaa tgccgcggca tgcgcggcat
     7021 tatgtagcca ggttggcaaa ttttagtgtc ttcagccatg tcttgcacgc tggatatcgc
     7081 gtatccgctg tttttctgcg cgagccatca gataccaggc gataaatccg acaattccga
     7141 ccgcaccgag aattaacgtt gcaagggcgt tgatttccgg attcactccc atccgcacgc
     7201 tggaaaagac cagcatcggt aacgtggtgg ctcccggccc ggaaacaaag ctggcgatca
     7261 ccagatcatc aagcgacaaa gtaaaagcca gtaaccagcc agaaatgatc gcgggcatga
     7321 tcatcggtag cgtaatgacg aaaaacactt tcagcggcgt cgcaccgaga tccatcgctg
     7381 cttcttctat cgagcgatcc agttcccgca atcttgaaga aataacgacc gccacataag
     7441 ccgtacaaaa agtgacatgc gccagccaga tggtgagcat accgcggtcc gcaggccagc
     7501 caatggcatg agcaagcgcg acgaataata acaacagcga caagcccgtg atgccatctg
     7561 gcatcaccag cggcgcggtg atcataaagg caaaaccatt tgatccgcga aacctgccaa
     7621 aacgcaccag caccaccgcc gcaatagtcc cgaggatcgc cgccgccgtt gccgcacagg
     7681 cagcaattgt caggcttaaa ccaaccgcac tcatcatcgc gtcatcgcgc aataactcac
     7741 cataccagcg cgttgaccag ccggcccaca ccgtcaccag cttcgagctg ttaaacgaat
     7801 agataaccag catcagcatt ggcgcgtaga gaaaggtaaa gcccagcagc aaaatcacaa
     7861 tccgccaggg cgaacgaact accggtaaat tattcatccg tgttctccca cgcttttttg
     7921 ctggtgtttg tggaaccaca ttatcggcac aattagcagc agcaacatga tgatcgctac
     7981 cgccgaggcc accggccagt cgcggttatt gaaaaactct tgccataaca cgcgcccgat
     8041 catgatgctg tccgggccac cgagcagttc cgggatcaca aactcgccca cagccgggat
     8101 aaacaccagc atcgatccgg caataatccc acctttagtc agcggcacga tcacagtaaa
     8161 gaacgttttc agcggtcgtg caccgagatc cagcgctgct tccaccagcg aataatcaat
     8221 acgaatcaac gcggtataaa tcggcagtac cataaacggc acgtaagcgt aaacaatgcc
     8281 gatataaacg gccagattgg tatgcagaat ggtcagcggt tgatcgataa cccccagcca
     8341 cagcagaaaa ttattcagca caccgttgtt ttttaatatt cccatccagg cataaacgcg
     8401 gatcagaaac gaggtccacg acggcaggat caccagtagt aataaaatat tacgggtcga
     8461 aggcttactg tgcgccaccg cccacgccag cggatagccg atcagtaaac agcaaaatgt
     8521 cgaaatcgcc gccacctgta acgactggag ataagcatca aaatagagcg gatcgtcggt
     8581 cagttgcaga aaattaccga gattaagagt gatggaaagt tgcccgtcag cccactccat
     8641 cagttcggta taaggtggaa tagcgcgcgc catctctccc aggcttattt taaagacaat
     8701 cagaaatggc agcagaaaca gcaagatcaa ccagatatat agcaacgcaa tgaccagttt
     8761 gcgcccatgc ttcatttgca gctgcgacaa ccacagctta aatccgcccg gcggtttcga
     8821 ctgggcagca ggttcaagtg tactcatctt tgctccttaa accgtcagca ccacacagct
     8881 gtccacttcc cagcacaaac gcacttcgtc gccccaggtc ggtaaccctt tacgatgacg
     8941 atgggcgttt tgtagctggg cgctgatcat ctgcccactt ttcagtcgaa cgtgatacac
     9001 cgaaagatcg ccgagatagg caatgtgtat cacctccccc accgcgaagt tacaaccatt
     9061 ggcgggcggc tcttcgcaaa gcatgatttt ttccgggcgc agcgctacgt gtaccggcac
     9121 gttatcgacc actgaagcat ccgcatcgac tttcagtgga tgcaccagcc ccggcgaatc
     9181 aagcaccagg ccatcttcct gacgctcttt gagtacacct tcaaagacat taaccgaacc
     9241 gataaattca gcgctgtagc gggtagttgg atgctcgtag atctcttccg gctcgccaat
     9301 ctggacaaat ttcccgcgat tcataatggc gatacgcccc gccatggtca tcgcctcttc
     9361 ctgatcgtgg gtgaccatca cacaagtcac accgacgcgc tccagaatat ccaccacttc
     9421 aagctgcatc cggtcacgca gctttttatc cagcgcgccc atcggctcat cgagcagtaa
     9481 tagtttcggg cgtttggcaa ggcttcgggc cagggccaca cgctgtcgct gaccaccgga
     9541 aagctgatgc ggtttgcgtt tggcgaactc ctgcatgtgc accaggccga gcatctcatg
     9601 gacccgactg gcaatttccg ctttcggtag tttgtcctgt ttcaggccaa aagcgatgtt
     9661 ctgttccacg gtcatatgcg gaaacagcgc gtaagactga aacatcatat tgatggggcg
     9721 caggtaaggc ggcacctgtg acaaatcgac gccatcgagc attatctgcc cggcagaagg
     9781 ttgttcaaaa cctgccagca tacgcagcag cgtggacttg ccacagccgg atgcgcccag
     9841 cagcgcgaag atttcacctt tgtagatggt caggctgaca tcatcgaccg catgctgacc
     9901 atcgtaggat ttggtgaggt tgcggatttc taatagcggc gtcagcgcct tacgggtttt
     9961 cgcctgcggg cgagggatag cgtcattcac ggctgctctc cggcattgaa tgtgtgcaag
    10021 cgcaccatga tggtgcgaat tgccggctgg tgtggtgcgc ccgcccggca tctaaggctg
    10081 cggattattt tccgctcttc actttggtcc acgcgcgggt gcgcacacgg tcgattttcg
    10141 gatcctgtac tttcagagtg aatagcttcg cacgaacgtc cgccggagga taaatacccg
    10201 ggttctcacg gacttccgca ctcaccagcg gcgtggctgc tttattggcg ttggcataga
    10261 acacatggtc ggaaatatgc gctactacat ccgggcgcag caggtaattc aggaactgat
    10321 aggcttcgtc tttgtttttg gcatccgcag gcatggcgaa tacatcaaag aacgccatcg
    10381 ccccttcttt tggaatcgag aacgagacat tcactccatt cttcgcttcc ttcgcgcggt
    10441 ttgacgcctg ccagacatca cctgcccagc cgatagcgac gcaaatatcg ccgtttgcca
    10501 ggtcgttaat gtattgagat gaatggaaat aacgaatgtt cgggcgcagc tttaacagca
    10561 gatcggttgc cggtccggtg taatcatccg ctttagtgct gttgggatct ttgccgagat
    10621 aattcaacac ggtagcaaaa acttcttctg gcgcatccag gaaagagaca ccgcagcttt
    10681 tcagtttttc cagattttca ggtttgagga tcaagtccca gctatcgacg ggcgcgtttt
    10741 cgcccagcac agctttaact ttatcaacgt tatagccaat cccggtcgtc gcccacatat
    10801 agggcatagc aaatttattg tcgggatcgt gtttggcgac cagcttcagc agctccgggt
    10861 cgagattctt ccactccggc aatttgcttt tgtccagcgg ctggaaaact cccgcagtca
    10921 actggcgttc aagaaagctg gcagatggaa ccaccaagtc aaagccggtg ctacccgcca
    10981 ttaatttgcc ttccagtact tcgttagagt cgaaaacatc gtagacgact ttaataccgg
    11041 tttctttttc aaaattggcc accgtgtccg gggcgatata atcagaccag ttataaatgt
    11101 ggagtgtttt ttgttcagcc gcgagcgtgc cgacagagac ggccatcaga gcacccgcag
    11161 ccagacccga aagccatttt ttatttaagg cggtcatatc tctttccttc tgaaagttcg
    11221 ttaacaaacg aacaaaagtt gtcacactaa aagatatgca ataaacgtgc atatttttta
    11281 tcggcggggt gtaagaaaag agaactctct cccggccagc actgctcgct atataaaagc
    11341 atcgcaagaa taactttagc cagggtttga gactatagct cggattaaaa aacggtattt
    11401 aacaaatttt tatgcgtatt tgctctatgt gataaagcca aatcgacaca agagaagtga
    11461 aataatcttt aagaaaaggt taaaaacagc agataatttt gcagcgcgca gccactgcac
    11521 cagcgactgc gtgctcgaaa ggagttatca gtggaggaaa tagttttcgg taacgttgtt
    11581 ttgcccttca tcgtcagtcg gtaacagcag ttgatgggca ttagcttcaa gaatgaccat
    11641 cgaaatctgc tcttcgctct gacgcacgaa ccatgcaaac tgctcatagg ttacgccctg
    11701 cataacggat aaagactggc aaaccaccag ctttggcaga ttatcatcct gcatatcaag
    11761 aaatgctttc acggtcagcg aactggcatt gatggcagat aaatccgccg ccagcggcaa
    11821 tacggctgaa ggtcggactt ccgccatggc agaaaacagg atcgtgttat cgatcagatc
    11881 gattttggca tcaaagacac cgtcgaaatt ctgcatatgg ggcagatgca gagcctgaca
    11941 gttatcacat tcaaaaaaac tcatccccag gtcatcgagc cattgacgca gcgtatccag
    12001 accaggaacg accagcgatg tcataatttt gtacaacctc tttcgataaa aagaccggca
    12061 cagcttacgc aaaaagcgca ggcaaaacca tgatcagtaa tgtgattgcg actaaccacc
    12121 cgttttcagg caatattctg tcgtagcgtg gcgttcgatc cagcggatca ttttacccgc
    12181 gatgtcgata ccagtggttt tttctattcc ttccagcccc ggcgacgcat tcacttccat
    12241 caccaacggc ccgcgattag cacgcagaat atccacacca gcaacgtcca gcgccatcgt
    12301 tcgcgcggct tttatcgcga tttcacgctc ctgtggtgtg atactggcga cgcttgccgc
    12361 gccgccacga tgcaaattgg aacgaaaatc gccctctttc gcccgccgtt caatcgcagc
    12421 gaccacttca tcgccaacaa ccagacagcg gatatcgcac ccttgcgcct ctttgatata
    12481 ttcctgcacc agaatatgcg cgttcagacc gcggaaagcg tcaatcacgc tttccgccgc
    12541 ctgacgcgtc tccgccagca cgacgccaat tccctgcgtg ccttcaacca acttgaccac
    12601 cagcggcgca ccaccgacca tgtcgattaa atcgctggta tcatccggcg aatgcgcaat
    12661 gcccgtgaca ggcaggtcga tgccctgacg cgccagcagt tgcatggaac gcaatttgtc
    12721 acgcgcccgg gcaatggcga ccgactcatt gagcggatag ctccccagca tctcgaactg
    12781 gcgcagtgcc gccgtcccat aaaaggtaat ggcggtgcca atacgcggga tcactgcgtc
    12841 aaaatggggt aacttgcggc ctttgtagtg aatagaagac gccgcaggat ttatgttcat
    12901 gtagcaagaa agcggatcaa gaatttcaac caggtgaccg cgctgtatag cggcttcacg
    12961 cagccgctta cacgaataga gcgttccatc ccgggacaat atggcaattt tcaccctgca
    13021 cctctctgtc agacctggtc aaaagcccgc gtatcataca tcgacgtggc ggttttagcg
    13081 cgtcgcccaa ccctgtttgt gcaaataatc cagaataaat gggcggcttt ctttaatgat
    13141 tgttcggcgg atatgatcgc tccaggtatc ccggcgatta ttgctgccac gggtgaggta
    13201 atattccgcc agttgctcgt catactgcgc cagtgcgtct ttatccagcg gttgatagct
    13261 gttttcatgc accaaaatgg aggccggtaa acgcggcttg agatccggat tatccgcagg
    13321 ccagccaagg cacagcccaa acagcggcag aacatgctgc ggtaatttca gcagtttcgt
    13381 caccgcttca atattattgc gcaggccgcc gatatatacc ccg
//
LOCUS       DAZOZD010000107        13410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOZD010000107 DAZOZD010000000
VERSION     DAZOZD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.107"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(36..119)
                     /locus_tag="ACHUPB_002518"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="ACHUPB_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUPB_002519"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHUPB_002519"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR0759302.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUPB_002520"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHUPB_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR0759303.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUPB_002521"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHUPB_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0759304.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUPB_002522"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="ACHUPB_002522"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0759305.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUPB_002523"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="ACHUPB_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0759306.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="ACHUPB_002524"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="ACHUPB_002524"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0759307.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUPB_002525"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="ACHUPB_002525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0759308.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="ACHUPB_002526"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="ACHUPB_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUPB_002527"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHUPB_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0759309.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="ACHUPB_002528"
     CDS             complement(12425..12664)
                     /locus_tag="ACHUPB_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0759310.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUPB_002529"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHUPB_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0759311.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="ACHUPB_002530"
     CDS             13041..13328
                     /locus_tag="ACHUPB_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0759312.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3489 a         3308 c         3458 g         3155 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc
//
LOCUS       DAZOZD010000108        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOZD010000108 DAZOZD010000000
VERSION     DAZOZD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.108"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUPB_002531"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUPB_002531"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0759313.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUPB_002532"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUPB_002532"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0759314.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUPB_002533"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUPB_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0759315.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUPB_002534"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUPB_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0759316.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUPB_002535"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUPB_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0759317.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUPB_002536"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUPB_002536"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0759318.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUPB_002537"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUPB_002537"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0759319.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUPB_002538"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUPB_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0759320.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUPB_002539"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUPB_002539"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0759321.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUPB_002540"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUPB_002540"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0759322.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUPB_002541"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUPB_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0759323.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHUPB_002542"
     CDS             13056..>13260
                     /locus_tag="ACHUPB_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0759324.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3365 a         3370 c         3489 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZOZD010000109        13245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOZD010000109 DAZOZD010000000
VERSION     DAZOZD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.109"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(496..1587)
                     /gene="ychF"
                     /locus_tag="ACHUPB_002543"
     CDS             complement(496..1587)
                     /gene="ychF"
                     /locus_tag="ACHUPB_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0759325.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1704..2288)
                     /gene="pth"
                     /locus_tag="ACHUPB_002544"
     CDS             complement(1704..2288)
                     /gene="pth"
                     /locus_tag="ACHUPB_002544"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0759326.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2565..2843
                     /gene="ychH"
                     /locus_tag="ACHUPB_002545"
     CDS             2565..2843
                     /gene="ychH"
                     /locus_tag="ACHUPB_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0759327.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2898..4577)
                     /gene="dauA"
                     /locus_tag="ACHUPB_002546"
     CDS             complement(2898..4577)
                     /gene="dauA"
                     /locus_tag="ACHUPB_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0759328.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4701..5648)
                     /gene="prs"
                     /locus_tag="ACHUPB_002547"
     CDS             complement(4701..5648)
                     /gene="prs"
                     /locus_tag="ACHUPB_002547"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0759329.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5799..6650)
                     /gene="ispE"
                     /locus_tag="ACHUPB_002548"
     CDS             complement(5799..6650)
                     /gene="ispE"
                     /locus_tag="ACHUPB_002548"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0759330.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6650..7273)
                     /gene="lolB"
                     /locus_tag="ACHUPB_002549"
     CDS             complement(6650..7273)
                     /gene="lolB"
                     /locus_tag="ACHUPB_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0759331.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7487..8743
                     /gene="hemA"
                     /locus_tag="ACHUPB_002550"
     CDS             7487..8743
                     /gene="hemA"
                     /locus_tag="ACHUPB_002550"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0759332.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8785..9867
                     /gene="prfA"
                     /locus_tag="ACHUPB_002551"
     CDS             8785..9867
                     /gene="prfA"
                     /locus_tag="ACHUPB_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0759333.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9867..10700
                     /gene="prmC"
                     /locus_tag="ACHUPB_002552"
     CDS             9867..10700
                     /gene="prmC"
                     /locus_tag="ACHUPB_002552"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0759334.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10697..11089
                     /gene="sirB2"
                     /locus_tag="ACHUPB_002553"
     CDS             10697..11089
                     /gene="sirB2"
                     /locus_tag="ACHUPB_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0759335.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11093..11901
                     /gene="sirB1"
                     /locus_tag="ACHUPB_002554"
                     /pseudo
     CDS             11093..11901
                     /gene="sirB1"
                     /locus_tag="ACHUPB_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            11937..12791
                     /gene="kdsA"
                     /locus_tag="ACHUPB_002555"
     CDS             11937..12791
                     /gene="kdsA"
                     /locus_tag="ACHUPB_002555"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0759336.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12940..13047)
                     /locus_tag="ACHUPB_002556"
     CDS             complement(12940..13047)
                     /locus_tag="ACHUPB_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0759337.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3300 a         3334 c         3424 g         3187 t
ORIGIN      
        1 gtatctgcat aacaaaaaca atatgcgcca tttttgcatt actaataaga aaaagcaaaa
       61 aacatcgata ttcatttttg gtcaatgggt tttctggcct atcgttgtta ttgtgctact
      121 ggttatcatt ttcatcttgc caggatcgca cgtaatcacg ttattcgggg actttatctg
      181 cgggatatta cccttcaggt aagtcctttt ctcgccagca tattggagcg tgatcaatat
      241 tgatcagctg tgaagggggt gatgtgtagt tgaacaaaaa cttacgttat atcactacta
      301 atgagatatc cgtcatctga cactgatgtc tttaattatt ctcgtggttc actgtaggca
      361 ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa tgaaaaatcc
      421 acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc gaaacctcat
      481 gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat ctttaacgat
      541 gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt cacctttgta
      601 agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt caaaatcagt
      661 atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc atgcacgcac
      721 ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac cgacacggat
      781 cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt cacgttcttc
      841 gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca caacagaacc
      901 ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa aaccgtcttc
      961 gttgacgttg gcgatgtaca ttgttggttt cagcgtcagg aagctcaggt aacgaatagc
     1021 cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt ccagctgggg
     1081 caggcatttt tctaggaccg ccagctcagc tttcgcgtct ttatcgccac ctttggcttt
     1141 cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg ccagttcggt
     1201 gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa tgatgttgtc
     1261 attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt tggtcaggaa
     1321 ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga tatcgacaaa
     1381 ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt gatccaggcg
     1441 agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg gaaagttggc
     1501 cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga cgttgggcaa
     1561 accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt aataatcaac
     1621 ctgttattgc taacagattg cagaaatgga aataactttg cctattatac acggcactcg
     1681 gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg gttcgttgct
     1741 ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc gatggcttca
     1801 tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac aactttattt
     1861 ttatcgccag gatgaccgat tccgatgcgt aaacggtgaa agttagggtt attacccaat
     1921 ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa tttaaatttg
     1981 gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc cggattaatg
     2041 cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa tgtagtcggg
     2101 actaacaggc ggacatcttc gcctccaaga gtgactcgcg aagtataacc aaagaattta
     2161 gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa ccaggcacca
     2221 gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac aatcaattta
     2281 atcgtcacgt tttttgtcct gagtgtgtac ataactggcg cgtagtttac tggttgcggc
     2341 cccgcttgac aaaaaactgc gtatcaaatg cagataacgt aataattgcc tgagtggact
     2401 attagaaagt caaggtgttc aggcgtttat ttgtaaagtt ttgttgaaat aagggttgta
     2461 attgtgatca cgcccgcaca taacccacag ggtgttgtct atactttaca cataaggaag
     2521 aggggtattc cctgttacaa cccagaaagt tccggaggtg acatatgaaa cgcaaaaacg
     2581 cttcgttact cggtaacgtg ctcatggggt tggggctggt ggtaatggtg gtcggcgtgg
     2641 ggtattcaat cctcaaccag ttaccacagt ttaatatgcc ccagtatttc gcacatggtg
     2701 cagtgctaag tattttcgtc ggtgccattc tctggctggc gggtgcccgt gttggcgggc
     2761 atgaacaggt gtgcgaccgt tactggtggg ttcgtcacta tgacaaacgt tgccgccgta
     2821 gcgataatcg ccgtcatagc taacaaataa tgcagtttgc tgaccagtca gtttgcgctg
     2881 actggtcaat ctcgtactta taaatccgcc atcgccgcgc gacgattcgg gaagaacgcg
     2941 aggcgtcccg ggatcggttg aatgcctgcg cgagccatag tgcgcagtgg ctggaattcc
     3001 acgttgcaca cgcgcagttc acatccttcg ggcagacgct tcacaaaacg ctggaacgca
     3061 tcaagaccac cagcatcaag taccggaacg gcatcccact tcagaatcac aatccgtttg
     3121 ccttcaagac gtgactccag gtccgtgaac aagccttcag cagcagcaaa aaacagcggg
     3181 ccaataacgc gcagtaccag aacatcgtct ggaacatcta cgactaccgg tgccaggcga
     3241 gtcatacgtg cgatacgacg cataaacagc agcgatgcca gcacgatccc cacgctgatg
     3301 gcaataacca tatcaaacag cacggtcagc gacatgcaca gcagcatgac aatgatgtca
     3361 tctttcggtg catgacgcag caagtcgacc actttatgcg cttcactcat gttccacgcc
     3421 accatcaaca gcagggctgc catagcggaa agcggcagcc aggagagcag cggtgccagt
     3481 accagcaggg caagaataac cagaatagag tggatcaccg ccgagatagg ggaagttgcc
     3541 ccggcacgga cgttagcggc agaacgcgcg atggcagctg tagcggtaat accaccaaag
     3601 aacggagcga taatattccc cagtccctgt ccaaccagtt cgctgttcgc cttgtgtttc
     3661 gtcccggtca taccatccag taccacggcg cagagcagag attcgattgc gccgagcatt
     3721 gccattgaga atgccgcagg cagcagtgtg cgaatagaat cccaggttag cgtgaattct
     3781 gaattaggca gatcccacgg cagcaccagt tgcggcagca gttgcggaat accgttaccc
     3841 tgagaaccat cggccaggac gtagtggaat tgcgaaccga tggtagcaac atgtccgccg
     3901 agcaggttaa caatccccat caccgcgcaa ccagccagca aggccggaag gtgaccgggt
     3961 aaacgaatgc ccagacgcgg ccaaaaaaca agaataccta gcgtcacaat gccaatggca
     4021 gcatcaccca cattaatggt cggcagcgcc ataaataatg cgccgacttt ttgtagataa
     4081 tgttccggga catgggccat ttgcagaccg agaaaatctt taatctgcat ggtaccgatg
     4141 gtgatcccga tacccgaggt gaaacctaag gtgacggaaa ccggaatata ctcaatcagg
     4201 cgaccaaagc gtgccagacc catcagaatc aaaaagatcc ccgacagcaa ggtcgcaacc
     4261 agcagtcctg ccagtccaaa ctgttgcgac acgggataga gaattaccac aaatgccgca
     4321 gtcggaccgg aaacgctaaa gcgtgaccca cccgtcagag caatgacaat ccccgcaaca
     4381 gctgcggtat ataaaccgta ctggggtgcc acaccactac caatagccaa cgccatcgcc
     4441 agcgggatag caataatccc gacggttatc ccggcaatca ggtcacgggt aaaccgtgcg
     4501 gcagtatatt tttctttcca gcaagcgtcg atcagagcgc ggaaaggcat cacatgtgag
     4561 gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc atcatctgtc aactaaatgg
     4621 caggtgaagg aggcataggt catacaaatg gatattacag acaaaaaaac ccgccgcagc
     4681 gggtctttga gcgggttcga ttagtgttcg aacatggcag agatcgattc ttcgttgctg
     4741 atacgacgaa tcgcttcggc cagcatacct gacagggtca gagtacgcac gttcggcagt
     4801 gatttgattt catcgctcag cggaatggta tcgcagacaa cgacttcatc aattacagag
     4861 ttacgcaggt tgttcgccgc gttgccagag aagatcgggt gagtcgcgta cgcaaataca
     4921 cgtttagcac cacgttcttt cagagcttca gcagctttac acagcgtacc gccagtgtcg
     4981 atcatatcat cgaccagtac gcagtcacga cctgcaacgt caccgatgat atgcatcacc
     5041 tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt atcgttcagc
     5101 agcttagcga tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac cacaattggg
     5161 ttatccagat tcagctgcag catgtcttcc agcaggatcg ggctaccaaa tacgttatca
     5221 accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt cagcacacgg
     5281 tcaacaccga cgctggagag gaagtctgca accactttcg cagtgattgg tacacgagcg
     5341 gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg ggataacagc ggtgatacga
     5401 cctgcggaag cacgacgcag ggcatcaacc ataacgacta attccatcag gttgtcgtta
     5461 gtaggggcac aagtggactg gatgatgaaa atatcaccac cgcgtacatt ttcgttgatt
     5521 tgtacactga cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag tgaagtgtac
     5581 aggcggttgg caatacgttg tgctagttcc ggggtggcgt taccagcaaa aagcttcata
     5641 tcaggcacga gaagaacctc aggcatgcgt ccattggtgg aaagaatctg ccgaaaactg
     5701 tgcgggccag gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc aacgtctgga
     5761 acaaggtgac gttgtcaccg aaactcagct tgcccggctt aaagcatggc tctgtgcaat
     5821 ggggaaagat taacaccttt cgccacaaag ccattgagcc attccggggc ttgctctagc
     5881 acctggcggg cttcagactc tgtatcaaat tcagcaaaga cacaggcccc tgtcccagtc
     5941 aggcgcgacg gggcgtattc taacagccag gaaagcaccg catcaacctc gcgaaaacgt
     6001 tttcttgcga taacctcgca atcattgctg aattcacatt ttagcaacgt ttctattgac
     6061 ctttttggcg tattgcgcgg gagttcagga tctttaaaaa tcactggtgt cggaatactt
     6121 acaccagggt gcgccaccag ataccacttc tctggcggat ccaccggcgt tagtatttca
     6181 ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc tgcgcccagc
     6241 gtcagcccca tttccgccag ctcatccatg cttagcccgc attgccagag atgatttaat
     6301 gccaccagga ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc catcggcaaa
     6361 cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct gtctgccgca
     6421 gttttcatca acaaccgcgc tgcgcgaacg atcaggttat cttcatgttc cacgccttca
     6481 acgggcgtta acagacgaat atccccatcg tcacgaagct caatgctgat ggtgtcgccg
     6541 taatcaagaa actgaaacag cgtttgcagc gtgtggtaac catccgcacg ctgaccggta
     6601 atgtataaaa acagattaag ttttgccgga gagggccact gtgtccgcat tatttcacta
     6661 tccagttatc catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc atattggctg
     6721 gcatcgcagg ttgcgttttg gtgtcataac caccataaac aaccttccag tttttgccat
     6781 tctggctgta ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag tcggttgcat
     6841 cacccggtaa acctaaaatc cattggcgca agctgttgag cggaattggc attccggtca
     6901 atttgccaat catctcttcg gcgtcatcgg cggtataacg ctgaccttta ttgtcgacta
     6961 actgcacgtt acccggttga gcattcagct ccagttccgt gctgcccaat gggttagtga
     7021 gcagcagacg gtagcgatcc tggccggttt gctgccagaa aaagcgggcg tacacttttt
     7081 gttggtcaga aatataagcg aacgcgccgc gagtctgata ctgattaaga ttgcgcacgt
     7141 cttgctgatg ctgacgccat tgtggcgaat ccgggctttt gccaggacct ttgggcgtgg
     7201 taacggaaca ggcagtgagc acaagagcag ccagcggtag caggcggata agacgaaaat
     7261 cgggctgggg catagtgatg acaagtcctt gagatacgtt gcagttataa cccttaatgc
     7321 tagcgttacc gtccgctatc gtctatgttc aagttgtctt aattgccaga atctaacggc
     7381 tttcggcaat tactccaaaa gggggcgctc tcttttattg atcttacgca tcctgtatga
     7441 tgcaagcaga ctaaccatat caacgttggt attatttccc gcagacatga cccttttagc
     7501 actcggtatc aaccataaaa cggcacctgt atcgctgcga gaacgtgtat cgttttcgcc
     7561 ggataagctc gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc agggcggcgt
     7621 ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc gttgaagagc aggataacct
     7681 gcaagaggcg ttaatccgct ggctttgcga ttatcacaat cttaatgaag aagatctgcg
     7741 taaaagcctc tactggcatc aggataacga cgcggttagc catttaatgc gtgttgccag
     7801 cggcctggat tcactggttc ttggggagcc gcagatcctc ggtcaggtta aaaaagcgtt
     7861 tgccgattcg caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt tccagaaatc
     7921 tttctctgta gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg ctgtgtctgt
     7981 cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa tcgctctcta cggtcacagt
     8041 gttgctggta ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc gcgaacataa
     8101 agtacagaag atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc tggcagatga
     8161 agttggcgcg gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg aagccgatat
     8221 catcatcagt tccaccgcca gcccgttacc gattatcggg aaaggcatgg tggagcgcgc
     8281 attaaaaagt cgtcgtaacc aaccaatgct gttggtggat attgccgttc cgcgcgatgt
     8341 tgagccggaa gttggcaaac tggcgaatgc ttatctttat agtgtggacg atctgcaaag
     8401 catcatttcg cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg aaactattgt
     8461 cgctcaggaa accagcgaat ttatggcgtg gctgcgagca caaagcgcca gcgaaaccat
     8521 tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca aagcgttagc
     8581 ggcccttgag cagggcggcg acgcgcaagc cattatgcag gatctggcat ggaaactgac
     8641 taaccgcttg atccatgcgc caacgaaatc acttcaacag gccgcccgtg acggggataa
     8701 cgaacgcctg aatattctgc gcgacagcct cgggctggag tagcagtaca tcattttctt
     8761 tttttacagg gtgcatttac gcctatgaag ccttctatcg ttgccaaact ggaagccctg
     8821 catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat cgccgaccag
     8881 gaacgttttc gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc gcgctgtttt
     8941 accgactggc aacaggttca ggaagatatc gaaaccgcac agatgatgct cgatgatcct
     9001 gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta aagaaaaaag cgagcaactg
     9061 gaacagcaat tacaggttct gttactgcca aaagatcctg atgacgaacg taacgccttc
     9121 ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc tgttcgcggg cgatctgttc
     9181 cgtatgtaca gccgttatgc cgaagcccgc cgctggcggg tagaaatcat gagcgccagc
     9241 gagggtgaac atggtggtta taaagagatc atcgccaaaa ttagcggtga tggtgtgtat
     9301 ggtcgtctga aatttgaatc cggcggtcat cgcgtgcagc gtgttcctgc tacggaatcg
     9361 cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa tgccagaact gcctgacgca
     9421 gaactgccgg acatcaaccc agcagattta cgcattgata ctttccgctc gtcaggggcg
     9481 ggtggtcagc acgttaacac caccgattcg gcaattcgta ttactcactt gccgaccggg
     9541 attgttgttg aatgtcagga cgaacgttca caacataaaa acaaagctaa agcactttct
     9601 gtacttggtg ctcgtatcca cgctgcagaa atggcaaaac ggcaacaggc cgaagcgtct
     9661 acccgtcgta acctgctggg gagtggcgat cgcagcgacc gtaaccgtac ttacaacttc
     9721 ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc tctaccgtct ggatgaagtg
     9781 atggaaggta agctggatat gctgattgaa ccgattatcc aggaacatca ggccgaccaa
     9841 ctggcggcgt tgtccgagca ggaataatgg aatatcaata ctggttacgt gaagcaataa
     9901 gccaacttca ggcgagcgaa agcccacgcc gtgacgctga aatcttattg gagtatgtca
     9961 ctggcagggg gcggactttt attctggcct ttggtgaaac gcagctgact gacgaacaat
    10021 gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt gctcatttaa
    10081 ccggggtgcg agaattctgg tcgttgccgt tatttgtttc gccagcgacc ttaattccgc
    10141 gcccggatac ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa caaccttgcc
    10201 gtattctcga tctcgggacg ggtaccgggg cgattgcgct tgcgctggct agcgagcgcc
    10261 cggactgcga aattatcgct gtagatcgta tgcctgatgc tgtctccctg gcacaacgta
    10321 atgcccagca tctggcgatc aaaaatatcc acattctgca aagcgactgg tttagcgcgc
    10381 tagccgggca gcagtttgcg atgattgtca gcaatccgcc gtatattgac gagcaggacc
    10441 cacatcttca acaaggcgat gtccgctttg agccgctcac tgcgctggtt gcggcagaca
    10501 gtggaatggc agacatcgtg catatcatcg aacagtcgcg taacgcgctg gtatccggcg
    10561 gctttctgct tctggaacat ggctggcagc agggcgaagc ggtgcgacaa gcatttatcc
    10621 tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg tgataacgag cgcgtaacgc
    10681 tcggccgcta ttatcaatga caagtttttc tacactgctt agtgttcatc ttattagtat
    10741 cgcgctttct gttgggctat taaccttacg tttctggcta cgttatcaga agcatccaca
    10801 ggcatttgct cgctggacgc gcattgtgcc gccggttgtc gatacggtgt tattgttaag
    10861 cggcattgcg ttgatggcta aagcgcacat cctgccattt tccgggcagg cacagtggct
    10921 gactgaaaag ctgtttggag ttatcattta tatcgttttg ggttttattg cactcgatta
    10981 tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc ccgctggcgt tggtggtgct
    11041 gtacatcatc attaaactcg ccaccacaaa agtaccgtta ctggggtaag tcatgagatc
    11101 gttagctgat ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc tggcttgcga
    11161 agcaatccgc cgcgattttc cctcgcaaga tgtttacgac gaactggagc gtctcgttag
    11221 tctggcgaag gaagaaatca gccagcttct gcctttagaa gagcagctgg aaaaactgat
    11281 cgcgctgttt tacggcgact ggggatttaa agcctcacgc ggcgtttatc gtctttccga
    11341 tgcattatgg ctggaccagg tgttaaagaa tcgacaggca gtgcggtatc attaggtgcg
    11401 gttttattat gggtcgcgaa tcgtctcgat ttgccgctgc tgccggtgat tttccctacg
    11461 cagctgatat tgcgcattga atgtccggat ggcgaaattt ggctgattaa tccttttaac
    11521 ggtgaatcgt taagcgaaca tatgctggac gtatggttaa agggaaatat cagcccgtcg
    11581 gcggaactgt tttatgaaga ccttgatgaa gctgataaca ttgaggtaat ccgcaaattg
    11641 ttggatacac tcaaagcctc gttgatggaa gaaaatcaga tggagctggc gttacgcacc
    11701 agcgaagctt tattacaatt caacccggaa gatccctatg aaattcgcga tcgcgggttg
    11761 atttatgcgc aactggattg cgaacacgtt gcgttgaacg atttaagtta tttcgttgaa
    11821 cagtgtccgg aagacccgat cagcgaaatg atccgtgcgc aaataaataa catcgcgcat
    11881 aaacatattg tgctgcatta attaatcgac attttactca agattaaggc gatcctatga
    11941 aacaaaaagt ggttagcatt ggcgacatca acgtagcaaa tgacctgccg ttcgtactgt
    12001 ttggtggtat gaacgtgctg gaatctcgcg atctggcgat gcgcatttgc gagcactacg
    12061 taaccgtgac ccagaaactg ggtatccctt acgtgttcaa agcctctttt gacaaagcca
    12121 accgctcctc catccactct tatcgtggac cgggcctgga agaagggatg aaaatcttcc
    12181 aggagttgaa gcagactttt ggcgtgaaaa ttatcaccga cgttcacgaa ccaagtcagg
    12241 cacagcccgt tgctgatgtc gtggatgtga ttcagttgcc ggcgtttctt gctcgccaga
    12301 ctgacctggt tgaagccatg gcgaaaaccg gtgcggtaat taacgtcaag aaaccacagt
    12361 ttgtcagccc gggacagatg ggtaatatcg ttgataaatt caaagaaggc ggcaacgaaa
    12421 aagtgattct ttgcgatcgc ggtgctaact tcggctatga caacctggtt gtcgatatgc
    12481 tgggcttcag cattatgaag aaagtgtctg gtaactcgcc ggtgattttc gacgtgaccc
    12541 acgcactgca atgccgcgat ccgtttggcg cagcttccgg tggtcgtcgt gctcaggtgg
    12601 ctgagctggc acgagccggt atggcggtag gtctggcggg gctgtttatt gaagcgcatc
    12661 cggatccgga acatgcgaaa tgtgatggtc catccgcgct gccgctggct aaactggaac
    12721 cgttcctcaa gcagatgaaa gcgattgatg atctggtgaa aggtttcgaa gaactggata
    12781 ccagcaagta atcttttttg cttgaaaaat aaagccttag cgttctgcgt taagactttt
    12841 ttcatgggtg ccagatacaa aaaaggccgc aggctgttac ccctgcggcc ggtttcgggc
    12901 gcatattgcc atcacggcag cctgacgccc gttttcacct tacttccggt tacgccacca
    12961 gccgacaatc gctgcggtaa taattcccgc caggatcggt gctgccaggt cgtgccagaa
    13021 aatcatggca aactgcgcga gcgtcatata gccgccttgt tgtaatgaca acattttgcg
    13081 gctattcttg aattgttctg gttcaagatt agcccccgtt ctgttgtcag gttaaacctc
    13141 tcaacgtgcg ggggttttct ctttccagca accaatgcca ccagggataa agcccccgca
    13201 acattgcgcc tcaccggata acgccggctt gatgtggata ctacg
//
LOCUS       DAZOZD010000110        13216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOZD010000110 DAZOZD010000000
VERSION     DAZOZD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.110"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            24..380
                     /locus_tag="ACHUPB_002557"
     CDS             24..380
                     /locus_tag="ACHUPB_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0759338.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(431..1327)
                     /gene="yhaJ"
                     /locus_tag="ACHUPB_002558"
                     /pseudo
     CDS             complement(431..1327)
                     /gene="yhaJ"
                     /locus_tag="ACHUPB_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1432..2133
                     /locus_tag="ACHUPB_002559"
     CDS             1432..2133
                     /locus_tag="ACHUPB_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0759339.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2156..2320
                     /gene="yhaL"
                     /locus_tag="ACHUPB_002560"
     CDS             2156..2320
                     /gene="yhaL"
                     /locus_tag="ACHUPB_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0759340.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2454..3764)
                     /gene="cyuA"
                     /locus_tag="ACHUPB_002561"
     CDS             complement(2454..3764)
                     /gene="cyuA"
                     /locus_tag="ACHUPB_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0759341.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3792..5123)
                     /locus_tag="ACHUPB_002562"
     CDS             complement(3792..5123)
                     /locus_tag="ACHUPB_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0759342.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5398..6762)
                     /gene="tdcG"
                     /locus_tag="ACHUPB_002563"
     CDS             complement(5398..6762)
                     /gene="tdcG"
                     /locus_tag="ACHUPB_002563"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0759343.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6834..7223)
                     /locus_tag="ACHUPB_002564"
     CDS             complement(6834..7223)
                     /locus_tag="ACHUPB_002564"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0759344.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7237..9531)
                     /gene="tdcE"
                     /locus_tag="ACHUPB_002565"
     CDS             complement(7237..9531)
                     /gene="tdcE"
                     /locus_tag="ACHUPB_002565"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0759345.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9565..10773)
                     /gene="tdcD"
                     /locus_tag="ACHUPB_002566"
     CDS             complement(9565..10773)
                     /gene="tdcD"
                     /locus_tag="ACHUPB_002566"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0759346.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10799..12130)
                     /gene="tdcC"
                     /locus_tag="ACHUPB_002567"
     CDS             complement(10799..12130)
                     /gene="tdcC"
                     /locus_tag="ACHUPB_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0759347.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12152..13141)
                     /gene="tdcB"
                     /locus_tag="ACHUPB_002568"
     CDS             complement(12152..13141)
                     /gene="tdcB"
                     /locus_tag="ACHUPB_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0759348.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3350 a         3593 c         3146 g         3127 t
ORIGIN      
        1 cttttaatta aagggatgtt tttatgcagt ggtatttatc cgtattaaaa aattatgttg
       61 gtttctctgg tcgcgcacgt cgtaaagagt actggatgtt tactctgatt aacgccatcg
      121 tcggcgctat tatcaatgtc attcaattga ttttaggtct ggagcttcca tatctgtcta
      181 tgctttattt gctggcaacc ttccttcccg tccttgcgct tgctatccgt cgcttacacg
      241 ataccgaccg ttcaggtgcc tgggccttgc tcttttttgt tccgtttatt ggctggctcg
      301 tgcttctggt ttttttctgc acagaaggta cttctggcag caatcgttac ggaaacgatc
      361 cgaagtttgg ttcaaattaa tcttagaatt ggggcgatat ttcgcccctt tttattaaca
      421 ataataaata ttattttccg ctaaaaagtt tgggaatttc ccgcagacac caggatttcg
      481 cttcccccat actgtcacga cgccaggcca taataatatc gatctcactg gtcgattccg
      541 ggctgacgac acgcaaccgc ccttccgcaa tatctttttc gaccatcgga tacggcatcg
      601 tcgccacgcc aagccccgcc agtaatgcct gacgtttatc ttcaatcgtg ctcaccgtta
      661 agcgcggctg tttgtccagc agttgtacgg tcaacaccgg gcgctcacga gcggtatccg
      721 ctaccgcaat tccacgatat ttcacgcgcg tcacttcaga taacggttcc ggctcctgat
      781 gaatcgggtg atcaggcgcg gcaacgtaga cgttcattaa cgtatagagc ttgcgcgagt
      841 tgatctccga cgaggaacga aaatgcatat ccggcgcgat aacaatatcc gcccgcccct
      901 gctccagtcg ttcctacgcc cccgccagca cttcggtgat gattgccagt tgggtattgg
      961 cttttgccgc cagtttgtcg attaacggga aaaaggcagg tgtcggtacc agcgcttcgg
     1021 tcacaatggt gagatgcgtt tcccaaccgc gcgcgagggc ttccgcatcg gtagtcagtt
     1081 tatctgcggc ttccagcaaa acgcgccctc gctccagcaa catcctcccg acattggtga
     1141 atttggtgcg atggcccgag cggtcaaaca gcaccacatc cagctcttct tccagttttt
     1201 gcatggtgta gctaagtgcg gaaggcacgc gtcccagctc atccgccgcc gccgcaaaac
     1261 tgccccggcg atcgatcgca tccataaccc gtagtgcttc cagcgttaat gccctttctt
     1321 tggccatttc gttctcattc aggaaatttg aacataccgg gcagaatatc tggctaacaa
     1381 tgtagcgtcc agccccttac cataaaagga agtaaagaga ggtcaagaat tatgattact
     1441 acccgaactg ccaggcagtg tggacaagca gactacggat ggttgcaggc ccggtatact
     1501 ttttcctttg gacactactt cgacccgaaa ttgttaggct atgcctccct gcgtgtgctt
     1561 aaccaggaag tgctggcccc aggtgccgcc tttcagccgc gaacctatcc caaagtcgat
     1621 attttaaatg tgattctgga tggagaagca gagtatcgcg acagcgaagg caatcatgtt
     1681 caggccagcg ccggtgaggc gttgctgctc tctacccagc cgggtgtcag ctacagcgaa
     1741 cacaatctca gcaaagacaa accgttaaca cgaatgcagc tttggctgga cgcctgcccg
     1801 cagcgagaga atccgctgat tcaaaaactg gcgcttaata tgggcaagca gcaattaatc
     1861 gcctcgccag agggtacgat gggaagcctg caattacgcc agcaagtgtg gctgcaccat
     1921 atcgtgctcg acaaaggcga aagtgcgaat ttccagttgc atgggccacg cgcgtatttg
     1981 caatcgattc acgggaaatt tcatgcgctt acgcatcatg aagagaaagc ggcgctgacc
     2041 tgcggtgatg gggcgtttat tcgtgacgag gctaacatta cgctggttgc cgattcccca
     2101 ctgcgcgctt tgctgataga tttgcctgtc tagttgtttt tacagggaga tgatgatgag
     2161 taaaaaatcg gccaaaaagc gccagccagt gaagcccgtg gtggcgaaag aacctgctcg
     2221 caccgccaaa aattttggct atgaagagat gttgagcgag ctggaagcca tcgtcgcgga
     2281 tgctgaaacg cgtttagccg aggatgaagc taccgcgtaa agacaggttg ccggatgcgg
     2341 cgaaaacgcc ttatccggcc tacaaaatcc aacaacatta acgaattaaa caacccgtag
     2401 gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg cgtttatctg
     2461 gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga atggcttgct
     2521 aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc ttcattgccg
     2581 gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga agcactggtc
     2641 gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg
     2701 atcatactgc tgatcgccat cgagatagtt tcataacgcc catccaccag ccatgccatc
     2761 ccggcggcgg cccccattgc tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac
     2821 tggttatgga tgtaaattgc gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca
     2881 tcatccgctc cgaagtgttc tgctacaacc accacaggca ttgttgcggt gatcccctgg
     2941 ttacccgagc cggagttact catagccgga agcgtagcgc cgcccatacg cgcatcggat
     3001 gccgcgctgg tacgaatcac aatggatgaa gagagatctt tcgccagcaa accgcgctcg
     3061 cactgttttt ccagcgtcgc gccaatatgc agcccccact taccgctcaa accttcctgc
     3121 gataacgcac aatttagctt cgcggaatcg agaataaagc ggatcgccgc aaacgggact
     3181 tcattgacga acttcaggat ctcagccagc gtcgttctgg aaagcaccgt cagcggagac
     3241 tcttgctcgc cctctgccac acacgcctgc tgggtaaaca ccacaccatt gtgcgtctca
     3301 atacgcacaa tgttggtatg cccgccgacg atggtgacac acgcccactt ctcaccgttc
     3361 cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt cctggatctt aacggagact
     3421 ttccccgccg ccagcagtgc tttggcatcg gcaattgcct gcgcagttgc gtctttcagc
     3481 acttccagcc cggcgttggc atttccacct aacgccccca gcgccgccgc aatcggcagc
     3541 cccaccattc ccgtgccggg aacggtgacg cccagaccgt tcttcatcag atttggcgaa
     3601 acccaggctt ctacacgttc aaccggacct tccagttctg ccgcagcaac cgccgccgcc
     3661 agcgccagtg aaatcggttc agtacatccc agcgccggtt ttacttcctc ctgaacggcg
     3721 aggatgtaac gctgccataa cggatttaaa gtcgaatcaa acatattaaa aaccttaaaa
     3781 tttcaggtaa atcaagaaaa tgccaggaac ggagaaacac aaagcaacaa accagtgacg
     3841 ataatcaggt acagagacat ccctttgtat ttgtgcaatg ccggtacttt gtaaaccagc
     3901 cacgccggga tcaggcaccc taccatgccg aaaatcgggc tacagataga ggtgaagctc
     3961 aacaccggtg cgttcagtac gatagcgctc caggccagca aaatggcgaa aatcatgatg
     4021 ccgcgctgaa caagattttc gttaatcttc tcggcaggca tcttgcgacg caggatgttc
     4081 attacgatcc cttgcgttgc ttcgcgaaag cctaaataga cgccaaagaa tgcagtcatg
     4141 acagcaaaga tattgagaat gacgctgacc actttcaccc acgctgcgcc gtcaccgcta
     4201 ataaactgtg cggcaatcgc cagcgcggaa atattctgct cgtaggcttt taccgcttcg
     4261 tcatgcccca tcgccagcgt gaacgacacg gcgtagaaaa agacggtgac aaacaaaatg
     4321 ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg ccacttcaat agatttttcc
     4381 cgcgagcgat aagagatcac catcgggctt aacgtctgga taaacaaaat cgacgtcagg
     4441 gtaaacggca gcgtaataat ggcgtttttc accagcagcc ccagcggcgg tagcgaaccg
     4501 acgttataca gatgccacat tcccaccatc gacacgccca gcgccgcgac caccagcagc
     4561 ttggtcagca ccatgccggt cgaaattttg aataacaatt tctcgccgcg tgaagagatc
     4621 gccaccagaa tgcaaatcag taccagacca taaaagggac tgtctgacag caacccttcc
     4681 gtcacgccga aggtatgcag ataggaagca ctatcgttgg tgatggcggt ggaatagacg
     4741 aacatccaaa tcaccagcat tacgaaatag agcgcaccta acaggatgcc ccagttttta
     4801 cctaaataac cgctaatgac actcgggtaa tctttacatt ctggtgattc tgccagcgta
     4861 ttaataaaca accgctgaaa cagatacatt gccgggtaac caatcaccga tgagagcaaa
     4921 aatacccaca atcccatcaa accgacctgc accgggagaa aaacaatccc cgcgccaatc
     4981 gccatcccga tactcataat cacccagccg gtgtcagtac tgtcgaattt gatcgcttct
     5041 cgccactcgc tctcactcat tccagcacga cccgccgggg tcgaagcgtc tgcaatgacg
     5101 cctttattcg atgcgatttc cataatttct cgctcaatat tttgtagggc ttattatttt
     5161 tttccgagcc gcatcaaggc gatatgcggt ctacgttttt gcaggcgagt tattagaata
     5221 aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat caagactttg aaataacgga
     5281 gatggataag aattttctac taaattaatc gcaagagaat attcattctc tatttgcggc
     5341 agatcacaaa aaaaaggtgc acatttgtgc acccaaggat gaaagctgac agcaatgtca
     5401 gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt tcgcggtatt tatcgttcat
     5461 atctttgccg gtttcataca tcgtctcgat cactttatcg agtgaaacac gcggtgccga
     5521 ggtgcggcgc atcgccatcc acgcggcgtt tactgctttc acggcattaa tggcattacg
     5581 ttcaatgcac gggatttgta cctgtccggc aaccggatcg caggtcagcc caaggttatg
     5641 ctccatcgcg atttccgccg cattgcatac ctgcgccgga ctgccgccca gtagttcagt
     5701 taaccctgcc gccgccattg aacaggccac gccaatctcc ccctgacagc cgacctccgc
     5761 gccagagatg gaggcgttca ttttatacag cgcgccaata gcccccgctg ccagaaaata
     5821 gcgggcaatt gaccgctcgt ttaccggacg acggaactta tcgtaataag ccagcactgc
     5881 cggaataatg ccgcacgcac cgttagtcgg tgccgttacc acgcgcccgc cagctgcgtt
     5941 ttcttcacta accgccagcg cgtacatgtt gatccagtcg atgacattca tcggatcgtt
     6001 agagatgtta tcgctagaaa ccagctgacg acgcagcgct acggcacgac gcggcacatt
     6061 gagcggacca ggcagcacgc cttcagtgtt catcccgcgt tcaataccgt catgcatcac
     6121 ttgccagata cgggcaaatc ctgcgtcaat ttccgctttg ctgcgcagcg ccagctcgtt
     6181 gtgcatcatc aggccagata tagacaggcc gttgtaatca cacattttca gcagttcacc
     6241 tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg tgcgacaggc cgaagtgttc
     6301 ttcttcgaca ataaacccgc cgccgacaga gtaataggtt ttacttaata gcgcttcctg
     6361 tcccttccag gcagtgatcc gcattccgtt ctcatggcga ggcaacattt cgggatggaa
     6421 gataatgttc tttgctacag gaaaatcaac aatatgcgca ccagatgcca ctggcagccg
     6481 cccgctgcgc gttactaact ctataaatgc agggatctca tcaatgacaa catcctgcgg
     6541 actgtttcct gccagtccca tgataatggc gacatccgtg gcatggcctt tgcccgtcag
     6601 tgacaacgac ccatacagat cgaccacaat atggctcgtc gcggttaata agccgctact
     6661 ttccagccga tcaataaaac tttttccggc attcattggc cccacggtat gcgaactgga
     6721 gggaccaatc ccaattttga aaatatcgaa tgcactaatc atatccacac cctcggattg
     6781 ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc cgctcacgag gctttacgca
     6841 ctacgtactg cgatagcttc aatttccagc ttcacatctt tcggcaaacg cgcaacctgc
     6901 acacagctcc gggtcgggta ggtcgcctga tgctcatcga agaactgctt atagacttca
     6961 ttgatggtgg caaaatcatt cagatcggtg atgaacacgg tcatcttgat gatatcgccc
     7021 acgctcagcc cggcagcaac cacgatcgct ttgacgtttt cgaggcttaa acacgcctga
     7081 tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa ccggaatttg cccggaggtg
     7141 aagaccatgc tgcctaaatc aacgccctga acataagggc cgattgcgcc tggggcacgt
     7201 tgcgtttcga taatcttttt catacatcct ccggcgtcag agcgcctggg taaaagtacg
     7261 tgaaataaca tcctgttgct gttcacgggt cagtgcgttg aagcgcacgg cgtagccaga
     7321 gacacggatt gtcaggttag gatatttttc cgggtgctcg atggcatcca gcagcatttc
     7381 ccgattcatt acgttgacgt tgaggtgttg accgccttcg acatccgctt cgtggtggaa
     7441 atacccatcc agcaggccga caaggttggt tttacgtact ggatcttctt tgcccagcgc
     7501 cgcaggaacg attgagaagg tgtacgagat cccatctttg gcgtaggtga acggcagttt
     7561 cgccaccgac gtcaatgagg ccacggcacc tttgcggtca cgaccatgca tcgggttagc
     7621 gcccggcgcg aacggtgttc cggcgcgacg accgtccggc gtattaccgg ttttctggcc
     7681 gtacaccacg ttagaagtga tagtcagaat cgactgggta gggacggcgt tgcgataggt
     7741 tggcagcgct ttaattttct tcataaagcg ttcaaccagg tcgcaggcaa tgctgtctac
     7801 gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg atttcaaagt ccaccgccag
     7861 gccgttttcg tcacggattg gtttcacgcg ggcatatttg atggcagaca gggagtccgt
     7921 cgccaccgac aggcccgcga tgccgcatgc catagtgcga tagacatcac gatcgtgcag
     7981 cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg tagtgaatga tattcagcgc
     8041 gctgatgtac tgcaccgcca gccagtccat gaagtgatcg aggctgtcca tcactttgtc
     8101 gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc ccgacctgaa tcttcagctt
     8161 ctcgtccacc ccgccgttaa ttgcgtacag cagtgttttc gccaggttag cgcgtgcacc
     8221 aaagaactgc atttgcttac caatcaccat tgggctgacg caacaagcaa ttgcgtaatc
     8281 gtcgctgttg aagtcagtac gcatcagatc gtcgttttca tactgcaagg aagaggtgac
     8341 gatcgacacc tgcgcggcat attttttgaa ggcaatcggt aattcttccg accaaagaat
     8401 ggtcaggtta ggttccggtg ccggccccat agtgtgcagg gtgtgcaaat agcggaagga
     8461 gtttttggtc accagcgtac gaccgtccag ccccatcccg ccgatcactt ccgtcgccca
     8521 gattgggtcg ccggagaaca gcgaatcaaa ttccggcgta cgcaagaagc gcaccatacg
     8581 gatcttcata atgaagtgat cgatcagttc ctgtgcctgc tgctcattga gtacgcctgc
     8641 tttaaagtcg cgctcaatgt agatatcgag gaacgacgcc gtgcgcccca gcgacatcgc
     8701 gccgccgttc tgtgatttca ctgccgccag ataagcgaag tagagccact gcaccgcttc
     8761 ctgcgcattc tgcgccgggc gagagatatc aaagccatat ttcgccgcca tttcctgaat
     8821 ctgcaacagc gcatgacgat gctctgccag ctcctcacgc agacggatgg tggcttccag
     8881 atcttcgcct ttttccagac gggactggag atcggcaaat tgcagttcgc gttcacgcac
     8941 cagataactg atgccataca gcgctacgcg gcgatagtca ccgataatgc gcccacggcc
     9001 atagccatct ggtaaaccgg tcagcacgcc agatttacgg cagcgcagca tatccggtga
     9061 gtaaacatca aatacgccct ggttatgggt tttacgcaga tcggtaaaca gatattcaaa
     9121 ttcactgtcc atttcacggc cataggcgtg gaatgaactt ttaatcatat taatgccacc
     9181 gaacggatgc agcgcgcgct ttaatggcgc atccgtttgc agaccaacaa ttttttccag
     9241 cggctggtta atatatcccg catcatgagc ggtaattgtg gtggcaatat tggtatcgaa
     9301 atcaaccggc gcgtgggttg cgttttcgat acggatgcct tccattactt tttcccacaa
     9361 ttccgtggtg gcaggcgtcg cttcggcgag gaaagattca tcgccttcat acggtgtata
     9421 gttatgttga ataaaatcgc ggacattaat ttcgtttttc cagtccgtac ctttaaagcc
     9481 aagccatgcg tcggcgtaca gcttatcgct ggtatcaata tctatcttca tgaaaaataa
     9541 tctctctaca atacttcaac taaattatgc aaattctgcg ggcgcgttaa ctttgcctaa
     9601 atgaatggca tccaaagcaa tcattttttc ttcgttagtc ggaataacgg cacaaatgac
     9661 acgcgcattt tcactggaaa caattcgctc tccaaaagag ttagagcgat tattcatttc
     9721 tgtatcaatc actacgccta atacagccaa atgttccatg accagacgac gaattaaact
     9781 tgaattctct cctattccgc cggtgaatat aattccatcc aggcgacgta atgaagctgc
     9841 gtgtccggca atatgacggg caattcggtg aacaaaggtt ttaattgcca gttgcgcgcg
     9901 ttcgtgacct tcatgccagg ctttttccag aacacgtaaa tccgaagaaa gaccggaaat
     9961 acctaataat cccgactctt tattcactac gcgttccagg tcacccaggc tctggttggt
    10021 ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg ccactgcggg ttcccatcat
    10081 caagccttcc agcggcgtca ttcccattga agtatcaaca ctctgaccgt tgcgaaccgc
    10141 gcagattgac gcgccattgc caagatgcgc cacaaccagg ccggagtcat cttccgccag
    10201 attcagcaga gaatgtgcgc gctgggaaac atagcggtgc gacgtgccgt ggaaaccata
    10261 acggcgtaca cctaactctt cataatattt ccacggcagg ccgtataaat aagcttccgg
    10321 agccatcgtc tggtggaaac tggtatcaaa taccgccacc tgagttacgc ccggaaataa
    10381 ttgctgcgcc gattcaatac cacttaaatt ggcgtaatta tgcaggggtg ccagtggaga
    10441 aacgcgacgg atattatcaa tgacttcatc ggtaataatg gcggactcgg taaaaatact
    10501 gccgccgtga gcgatgcggt ggccaattaa ggccacactg tcatttaaat tccgtttttc
    10561 cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg tggtgagcca gcggtgctgg
    10621 ctctccccca tttacggata agaatgcatt ttccgagtta ataccgtcgg caatacctga
    10681 cattaatact tcacagtcgc tggcatcgag cacggaaaac ttaatcgaag acgaaccaca
    10741 gttaataacc aaaacaaccg gaaattcatt catctctttt ctcatcctga gttacggatt
    10801 aaaacagttt gtatacgatg ttcaggatgg tcagcagacc aatcacggta acaaacacgt
    10861 tatccagacg accacggtat ttcgccagag acggcgcttt acggatggca tacatcggca
    10921 acaggcacag cagggatgcg ataatcggtg cgcccatggc ttcaatcagg tcgaggatgt
    10981 tcgggttggc gtaggcaaca acccaggtgg agcccatgat gaagatcatg ctgatagtgt
    11041 tcagtttacc cagcgacact ttagttttgt cgcctttata accaaacttc aggaccaggc
    11101 cattcagacc ttccagcgtt cccagatagt gaccgaagaa agatttgaag atagccacga
    11161 gtgcgatgat ggaagccgca tattccagtg taatcgcgaa cgttgttttg gtaccggtca
    11221 tggacgcaaa gtggttagcc agataagaaa gcactggaat attctgcgct ttggcttccg
    11281 ccatgttggc cggagacagc gtaaataggc agctaaaggc aaagaacatc accactgcaa
    11341 ccatcagcat gctggcacga gaaatgattt gggaacattt acgttcggtg aagtcgcgac
    11401 cgaagtcttt ctcatactct tcacgtttag aaaccacgaa ggaagagacg attggcgaga
    11461 aattaaagga gaaaaccatg atggaaatcc ccagccagac ggtgatcagg ataccgtcat
    11521 gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat aactgcagag ttccagtaag
    11581 ggatcagcga cagagaaatc agcaccaggc tggcgataaa cggccatacc aggtagctca
    11641 tcactttaac catcagatcc ttaccaaacc agatgacgaa agccatcagc agcagcagga
    11701 acagcgccac aaagccgcga ttcagcggtg caaagccgag ctggttttcc cagaacgtca
    11761 taaaggtatt ggtaatagta acgccataaa tccacagcag tgggcaaatc gcgaagaagt
    11821 acaggaacgt gataaccacg ccgccagttt taccaaaatg ctcttccacc gtttccgtaa
    11881 tgttgccgga agggttagag ccagaaagac atagacgcgc cagcgcccgg tggcaataaa
    11941 atgcgatggg gtatgccaat accaacatca gaagaatcgg gatcagtccg ccaaaacctg
    12001 cgcggatagg gaagaacagc accccggcgc cgattgccgt accaaacaag cctaacgtcc
    12061 atgtggtatc tgatttacgc caggacgatt gttttgtctg gctggataca atgctatctg
    12121 aagtactcat atcctatcct caacgaatta attaagcgtc aacgaaaccg gtgatttgag
    12181 agacgcgaga aagatcgata ttgccgccgg aaataatact gacggttttt ctgttttgaa
    12241 tatattggtc taatttaccg cttaataatg cagcacatgc cagagcgcct gcgccttcgg
    12301 tgacgacttt attgcgctga attaaggcaa tcatactgtt tctgatttcg tcttcgctga
    12361 ccagcacgat gtcatcgact aattcacgaa cgatttcgta agttaaatta cccgggcggg
    12421 agacatcaca accatccgcc agggtgccgg tagttcggtg cgtggttatt tctccggagt
    12481 ggaaagaagc cgccatgccg tgaacgtttt cagactgtac gccaataaca cgaatggtcg
    12541 ggttaataga tttaattgcc accgcaatac cagcaattaa accgccacca ccaattggca
    12601 caatcacgtt atcgacatca tagagatctt ccataatttc cagaccaatc gttccctggc
    12661 cagcaatcac tttcggatca tcgtaaggtg ggataaaaat acggccttcc atttcgacaa
    12721 tttcgctcac tttagcgata gtgtcgttga agttatcacc atgcagaacg acttctgcgg
    12781 agtagtcgca cgttgccgct actttggatt ttggcgcacc ttttggcatc accactttac
    12841 cgtcgatacc cagcatcgcg caggagagcg aaaccccttg cgcatggttg cccgcagaac
    12901 aggccaccac gcctttgcgt ttttccgcat cggtcagtga acttaattta ttaaatgcgc
    12961 cacgaatttt aaatgaaccc gtacgctgca tattttcaaa cttcaggaat atttcacctt
    13021 tgcaacgttc actaaaatag ttggagcgag gcatgcctgt tttataaatt cgcccagcca
    13081 gtcgttgttt cgcttcaata atatcatcaa tagcaatcgg cagatcgtat gtaatatgca
    13141 ttataaaacc tcttcgcctg aattaaatat gtaggtaacc gtaaccgaca cctgcaaaac
    13201 agacaggtgg attatt
//
LOCUS       DAZOZD010000111        13138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOZD010000111 DAZOZD010000000
VERSION     DAZOZD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.111"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            166..465
                     /locus_tag="ACHUPB_002569"
     CDS             166..465
                     /locus_tag="ACHUPB_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759349.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(512..1399)
                     /gene="rarD"
                     /locus_tag="ACHUPB_002570"
     CDS             complement(512..1399)
                     /gene="rarD"
                     /locus_tag="ACHUPB_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0759350.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1451..1918)
                     /gene="yigI"
                     /locus_tag="ACHUPB_002571"
     CDS             complement(1451..1918)
                     /gene="yigI"
                     /locus_tag="ACHUPB_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0759351.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2083..2952
                     /gene="pldA"
                     /locus_tag="ACHUPB_002572"
     CDS             2083..2952
                     /gene="pldA"
                     /locus_tag="ACHUPB_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0759352.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3085..4914
                     /gene="recQ"
                     /locus_tag="ACHUPB_002573"
     CDS             3085..4914
                     /gene="recQ"
                     /locus_tag="ACHUPB_002573"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0759353.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4978..5595
                     /gene="rhtC"
                     /locus_tag="ACHUPB_002574"
     CDS             4978..5595
                     /gene="rhtC"
                     /locus_tag="ACHUPB_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0759354.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5657..6277)
                     /gene="rhtB"
                     /locus_tag="ACHUPB_002575"
     CDS             complement(5657..6277)
                     /gene="rhtB"
                     /locus_tag="ACHUPB_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0759355.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6388..7410
                     /gene="pldB"
                     /locus_tag="ACHUPB_002576"
     CDS             6388..7410
                     /gene="pldB"
                     /locus_tag="ACHUPB_002576"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0759356.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7418..8218
                     /gene="yigL"
                     /locus_tag="ACHUPB_002577"
     CDS             7418..8218
                     /gene="yigL"
                     /locus_tag="ACHUPB_002577"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0759357.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8294..9193
                     /gene="bioP"
                     /locus_tag="ACHUPB_002578"
     CDS             8294..9193
                     /gene="bioP"
                     /locus_tag="ACHUPB_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0759358.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9081..10034)
                     /gene="metR"
                     /locus_tag="ACHUPB_002579"
     CDS             complement(9081..10034)
                     /gene="metR"
                     /locus_tag="ACHUPB_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0759359.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10270..12531
                     /gene="metE"
                     /locus_tag="ACHUPB_002580"
     CDS             10270..12531
                     /gene="metE"
                     /locus_tag="ACHUPB_002580"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0759360.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12653..12775)
                     /locus_tag="ACHUPB_002581"
     CDS             complement(12653..12775)
                     /locus_tag="ACHUPB_002581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759361.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12816..13061)
                     /locus_tag="ACHUPB_002582"
     CDS             complement(12816..13061)
                     /locus_tag="ACHUPB_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0759362.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3112 a         3255 c         3498 g         3273 t
ORIGIN      
        1 ttatgtggtt tgcatatata tatgcaatat agcagtcttt gcattgataa ctatacagta
       61 gtaatttaga gtgtgtaagt ttttattgat ggggtatatt ttaattaaat ataattgaaa
      121 tgtattttta ttctataaac aatatcttgg gtgaatggat tctatatggg aaatggtaac
      181 ttttggcagt tgttcttggg taaagaacct tacagcaaaa aatctgattt tgttaaggca
      241 tggttattgt ggtgggctat taccatcctt gttctattta ttttaagcct gactacgata
      301 ttaaatttaa gaagtatagg tatagggtta agttttgggt tttgtcaaat ccttggtaga
      361 tatacgatgt ctctaggatg gagtaagtac tggggagttt tgggggtatt tcctgtaaca
      421 aatattgttc tttttcttat cttaattttt ttcaaaaaaa gataaataag tcagtgggta
      481 ggctctttaa acggcctacc catgttaatt cttaactttt tctacgctgc gtataaatcg
      541 catccatcac aaaaattgcc agcgccaccc aaataaaggc gaaagtcacc atcttatcgg
      601 cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc gggccaatgt
      661 actggaaaaa gcctaacgtt gagagacgca agcgcgtggc agcggcggta aaacacaaca
      721 gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac atcgggtttt
      781 gccccatatg gctggttgag ctgtcggcaa tagcaaacag gtaaattgcc gccacgggca
      841 gcagccacat ggtttcgatt aacatgccgg tttgcgcttc aacggcaatc ttcttgcgta
      901 ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc gataataggt agcgaaccaa
      961 aagtccacag ctggactaac acgccacata tcgccagaat caccgccagc cattgcatcc
     1021 ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc gggttaataa
     1081 agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata aacagtagcc
     1141 agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt ggcgtctgaa
     1201 tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc ataaagaaaa
     1261 acgaccagat cacgcgatgc gtcaggattt catcggcggg cacgtagtaa atcaacttga
     1321 agtacgctgg cgctataccc caaataaaat aagcggcaag agcgagtaat acgccctgcc
     1381 gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca gtttacctgc
     1441 ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa agctgttctt
     1501 cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac aggctactgg
     1561 tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga tcaatggtcc
     1621 ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg cgggttaagg
     1681 tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg accccgccgt
     1741 gcaaaatgct ttgcgcccag ttgcccacca tcattggctg atttttaaag gccagctgtg
     1801 cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat ggcatgtgat
     1861 aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg gcagacatat
     1921 catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt tgttggtttc
     1981 tactttagga agggataact aacggctatg gagttaagta tgtaaaatag cccgcagaaa
     2041 aatattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac tctgcagggc
     2101 tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc aacggtgaaa
     2161 gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct gcaggagcat
     2221 gacaatccgt tcacgctcta tccttatgac accaactacc tcatttacac ccaaaccagc
     2281 gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg taaggatgaa
     2341 gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg cccgaactcg
     2401 gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag cgaagagtct
     2461 tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc caccgattac
     2521 tgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga ctctaacggg
     2581 cgttccgacc cgacctcccg cagctggaac cgcctttata ctcgcctgat ggcagaaaac
     2641 ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga cgataacccg
     2701 gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct cggtgatgcg
     2761 gtgttgagtg cgaaaggaca gtacaactgg aataccggtt acggcggcgc ggagttaggc
     2821 ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag cggctatggc
     2881 gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtaggagt tatgctaaac
     2941 gacttgtttt gatgaacggt tgagtggttg gcaaatctgg aacccagcat ccaggattac
     3001 cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata gcgcctgttt
     3061 ttatttcagg caatcggggg gaatgtggcg caggcggaag tgttgaatct ggagtccgga
     3121 gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg ccaggaagaa
     3181 attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg tcatgcccac cggtggcgga
     3241 aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt ggttgtttca
     3301 ccgctgattt cgttgatgaa agatcaggtg gatcaactgc aagccaacgg cgtggcggcg
     3361 gcgtgcctta actcgacgca aacccgcgag cagcaacttg aagtgatgac aggctgccgc
     3421 accgggcaaa ttcgtctgct ttatatcgcc ccggaacgcc tgatgctgga taactttctt
     3481 gagcatctgg cgcactggaa tccggtgtta ttagccgttg atgaagcgca ctgtatctcc
     3541 caatggggcc acgatttccg cccggaatat gccgcgcttg gtcagttacg ccagcgtttc
     3601 ccgaccctgc cgtttatggc gctgactgcc acagccgacg acaccacgcg ccaggatatc
     3661 gtgcgcttgc tggggctgaa cgatccgctg attcaaatca gcagttttga ccgtccgaat
     3721 attcgctaca tgctgatgga gaaattcaaa ccgctcgatc agttgatgcg ctacgtgcag
     3781 gaacagcgcg gtaagtccgg cattatctac tgcaacagcc gggcgaaagt agaagacacc
     3841 gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg gctggaaaat
     3901 aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg acctgcaaat tgtggtggcg
     3961 acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt ccactttgat
     4021 attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg tgatggcctg
     4081 cctgcggaag cgatgctgtt ttacgatccg gctgatatgg cgtggctgcg ccgttgtctg
     4141 gaagagaagc cgcaggggca gttgcaggat attgagcgcc acaaactcaa tgcgatgggc
     4201 gcgtttgccg aagcgcaaac ttgccgtcgt ctggtattgc tgaactattt tggcgaaggg
     4261 cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca gtacgacggt
     4321 tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg gtttgggatg
     4381 ggttatgtgg tggaagtgat tcgtggtgct aataaccagc gtatccgcga ctatggtcat
     4441 gacaaactga aagtctatgg catgggccgt gataaaagcc atgaacattg ggtgagcgtg
     4501 atccgccagc tgattcacct cggcctggtg acgcaaaata ttgcccagca ttctgcccta
     4561 caactgacag aggccgcgcg cccggtgctg cgcggcgaat cctctttgca acttgccgtg
     4621 ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg caactatgat
     4681 cgtaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga aagtaatgtc
     4741 ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca gatgccgatc
     4801 accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga acgctttggt
     4861 aaaccgttta tggcgctgat tcgtgcgcat gtcgatggcg acgacgaaga gtagtcagca
     4921 gcataaaaaa gtgccaggat gaagactccg taaacgtttc ccccgcgagt caaatgtatg
     4981 ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag ccccggtccc
     5041 gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc gatgatgggc
     5101 gtgctgggca ttacctgcgg tgtaatggtt tgggctggga ttgcgttgct tggcctgcat
     5161 ttgattatcg aaaaaatggc ctggctgcat acgcttatgg ttggcggtgg cctgtatctc
     5221 tgctggatgg gttaccagat gctgcgcggc gcgctgaaaa aagaggcggt ttctgcacct
     5281 gcgccacagg tcgagctggc gaaaagtggg cgcagtttcc tgaaaggttt actgaccaat
     5341 ctcgctaatc cgaaagcgat tatctacttt ggctcggtgt tctcattgtt tgtcggtgat
     5401 aacgttggca ctaccgcgcg ctggggcatt tttgcgctga tcattgtcga aacgctggcg
     5461 tggtttatcg tcgttgccag cctgtttgcc ctgccgcaaa tgcgccgtgg ttatcaacgt
     5521 ctggcgaagt ggattgatgg ttttgccggg gcgttatttg ccggatttgg cattcatttg
     5581 attatttcgc ggtgatgcca gacgcgtctt cagagtaagt cggataaggc gtttacgccg
     5641 catccgacat tatttttcac gcatgcctcg ccgatgctaa cagcgctccc accagcataa
     5701 acaacgagcc gaaaatctta ttcagcgcct tcatctgctt tggtccttta atccatagag
     5761 caatccgttg agcaagggtg gcgtaaccga tcatcacaat aatatcgacc acaatagtgg
     5821 tgacgccgag cacgatatac tgcatcagtt gcggctgttg cggcatgatg aattgcggaa
     5881 atagcgccgc cagaaacaca atacttttgg gattggtgag attcacaaaa actgcgcgct
     5941 ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt aaggtcaatt gcaccagcgg
     6001 cgcgccactg ctggattccc agccaaatca agtaagccgc gcctgcccac ttcaacactt
     6061 caaacgcaat cactgagcgg gaaaatagcg tccccaaccc cacgccaacc agcacaatat
     6121 gaatcgccag tccggtctga agcccagcaa tagacgccac cgcgccgcga taaccgtggt
     6181 tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg cgacaggctt aaaatgatcg
     6241 atgtcagcag gtaggcaaac caccattcta aggtcatgat gaactcccgg tgtgtctatt
     6301 tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg cacgctaaaa aaaacgattt
     6361 tacgtggttt aagaggcaga ttacccgatg tttcagcagc aaaaagactg ggaaacaaga
     6421 gaaaacgcgt ttgctgcttt taccatggga ccgctgactg atttctggcg tcagcgtgat
     6481 gaagcagagt ttactggtgt ggatgacatt ccggtgcgct ttgtccgttt tcgcgcacag
     6541 caccatgacc gggtggtagt catctgcccg gggcgtattg agagctacgt aaaatatgcg
     6601 gaactggcct atgacctgtt ccatttgggg tttgatgtct taatcatcga ccatcgcggg
     6661 cagggacgtt ccggtcgcct gttagccgat ccgcatctcg ggcatgttaa tcgctttaat
     6721 gattatgttg atgatctggc ggcattctgg cagcaggagg ttcagcccgg tccgtggcgt
     6781 aaacgctata tactggcaca ttcgatgggc ggtgcgatct ccacattatt tctgcaacgc
     6841 catccaggtg tatgtgacgc cattgcgcta actgcgccaa tgtttgggat cgtgattcgt
     6901 atgccgtcat ttatggcacg gcagatcctc aactgggccg aagcgcatcc acgtttccgt
     6961 gatggctatg caataggcac cgggcgctgg cgcgcgttgc cgtttgctat caacgtactg
     7021 acccacagca gacagcgata tcgacgtaac ttacgcttct atgctgatga cccaacgatt
     7081 cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta ttctggctgg cgaacaggtg
     7141 ttagccggtg cgggtgatga cgccacgcca acgcttctct tgcaggctga agaggaacgc
     7201 gtggtggata accgcatgca tgaccgtttt tgtgaactcc gcaccgccgc gggccatcct
     7261 gtcgaaggag gacgaccgtt ggtaattaaa ggtgcttacc atgagatcct ttttgaaaag
     7321 gacgcaatgc gctcagtcgc gctccacgcc attgttgatt ttttcaacag gcataactca
     7381 cccagcggaa accgctctac agaggtttaa atttcttatg taccaggttg ttgcgtctga
     7441 tttagatggc acgttacttt ctcccgacca tacgttatcc ccttacgcca aagaaaccct
     7501 gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg accggtcgtc accacgttga
     7561 tgtggggcaa attcgcgata atctggagat caagtcgtac atgatcacct ccaacggcgc
     7621 gcgcgttcac gatctggatg gcaatctgat ttttgctcat aacctggatc gcgacattgc
     7681 cagcgatctg tttggcgtag tcaacgacaa tctggacatc attactaacg tttatcgcga
     7741 cgacgaatgg tttatgaatc gccatcgccc ggaagagatg cgctttttta aagaagcggt
     7801 gttcaaatat gcgctgtatg agcctggatt actggagccg gaaggcgtca gcaaagtgtt
     7861 cttcacctgc gattcccatg aacaactgct gccgctggag caggcgatta acgctcgttg
     7921 gggcgatcgc gtcaacgtta gtttctctac cttaacctgt ctggaagtga tggcgggcgg
     7981 cgtttcaaaa ggccatgcgc tggaagcggt ggcgaagaaa ctgggctaca gcctgaagga
     8041 ttgtattgcg tttggtgacg ggatgaacga tgccgaaatg ctgtcgatgg cggggaaagg
     8101 ctgcattatg ggcagtgcgc accagcgtct gaaagacctt catcccgagc tggaagtgat
     8161 tggtactaat gccgacgacg cggtgccgca ttatctgcgt aaactctatt tatcgtaatc
     8221 gttctttatt tggtcagttg tcaacctgat acttcgctac aatggatact cgttaatcaa
     8281 agagttttcc attgtggcgc tacttatcat caccacgatt ctgtgggcct tctcctttag
     8341 cttttatggc gagtaccttg cggggcacgt cgatagctat tttgcggtgc tggtgcgcgt
     8401 tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc cgtggcaata gcctgaaaac
     8461 ggtcggcctg tatatgctgg tgggcgcgat gcagctcggc gtgatgtata tgctgagttt
     8521 ccgcgcttat ctctacctga cggtctccga attgttgctg ttcaccgtac tgacgccgct
     8581 ctacatcacg ctgatttatg acatcatgag taagcgccgt ctgcgctggg gctatgcttt
     8641 tagcgccttg ctggcggtga ttggtgccgg gattattcgc tatgatcagg ttaccgacca
     8701 tttctggact ggcttgttgc tggtgcaact ctccaatatc acttttgcca tcggcatggt
     8761 gggttacaaa cgcctgatgg aaactcgccc gatgccgcag cataacgcct ttgcgtggtt
     8821 ctatcttggc gcgttcctgg tggcggtaat tgcatggttc ttgctgggaa atgcgcagaa
     8881 aatgccgcaa accacgttgc aatggggcat tctggtgttt cttggcgtgg tggcttccgg
     8941 gattggctac tttatgtgga actacggcgc gacgcaggtg gacgccggaa cgctgggcat
     9001 tatgaataat atgcacgttc cggcagggct gctggtaaac ctggctatct ggcaccaaca
     9061 gccgcactgg ccaacgttca ttacaggcgc gctggtgatc ctggcctcac tgtgggtgca
     9121 tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat gatcgcaggc gtgattgcgc
     9181 gctgagcgaa taaatgcttc tgtaatcggc tgacgctgct cgccatcgcg cacggcggcg
     9241 tacagtcggc tccacaagcc ttcgcccagg gttttggtca ccaccagacc ttggcgctca
     9301 aaactctcta ctacccaatg cggtagcgcg gcaataccca tccgcgcggc aaccatctga
     9361 atcaacaata aggtgttatc gacgcttttc agtgacgggc tgacgcctgc cggctgaaga
     9421 aaatgccgcc agacatccag tcgactacgc tgcaccggat aaatcaacag cgtctcgctg
     9481 gcgagatctt ccggtgtaat tcgcgttttc gccgccagtg gatggtcagg tgctaacacc
     9541 agacgcactt catagtcgaa catcggcgaa taatgcaggc cactgcgcgg caggatatcg
     9601 gaggtcatca ccagatccag ctcgccctgt tgcaacgcag gctgcgggtc aaacgtcacg
     9661 cccgatttaa aatccatctc tacctgcggc cagttcttat ggaaattttc taacgcgggc
     9721 gtcagccact gaatacagct atggcactca atggcaatgc gcagacgcgt ctgctgcggt
     9781 tcattgcagg cttgcagtgc ctggctgatt tgcggcagca cctggtttgc cagttgcaac
     9841 aggatttctc cctgcggtgt aaagcgtagc ggctggctct tacgcacaaa tagccggaag
     9901 ccaaggcgtt gttccagatc gctaaactgg tgagacaggg cggattgcgt ctgatgcaac
     9961 gtcgccgcag cggctgcgag agagccgcag ttccgcaacg cttgtagcgt tttcaggtgt
    10021 tttacttcga tcatgaaagt ccttcacttc ggcatgaata atttgcgctt gaggaatata
    10081 cagtaaccgc caattatgga tgtgtaaaca tctggacggc taaaatcctt cgtcttttaa
    10141 atttatggtg cgttggctgc gtttctccac cccggtcact tacttcagta agctcccggg
    10201 gatgaataaa cttgccgcct tccctaaatt caaaatccat aggatttaca tataattaga
    10261 ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc
    10321 gagctgaaaa aagcgcaaga aagttattgg gcggggaact ccacgcgtga agaactgctg
    10381 acagtagggc gtgaactgcg tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac
    10441 ctgctgccgg tgggcgattt tgcctggtac gatcatgtac tgaccaccag tttgctgctg
    10501 ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg tagatatcga caccctgttc
    10561 cgtattggtc gtggacgtgc gccgactggc gaacctgcgg cggcagcgga aatgaccaaa
    10621 tggtttaaca ccaactatca ctacatggtg ccggagttcg ttaaaggcca acagttcaaa
    10681 ctgacctgga cgcagctgct ggaggaagtg gacgaggcgc tggcgctggg ccacaacgtt
    10741 aagcctgtgc tgctggggcc ggttacttac ctgtggctgg ggaaagtgaa aggtgaacag
    10801 tttgaccgcc ttagcctgct gaacgacatt ctgccggttt atcagcaagt gctggcagaa
    10861 ctggcgaaac gcggcatcga gtgggtacag attgatgaac ccgcgctggt actggaacta
    10921 ccacaggcgt ggctggacgc atacaaaccc gcttacgacg cgctccaggg acaggtgaaa
    10981 ctgctgctga ccacctattt tgaaggcgta acgccaaatc tcgacacgat tactgcgctg
    11041 cctgttcagg gtctgcatgt tgatctcgta catggtaaag atgacgttgc tgaactgcac
    11101 aagcgcctgc cttctgactg gttgctgtct gcgggtctga tcaatggtcg taacgtctgg
    11161 cgcgccgatc ttaccgagaa atatgcgcaa attaaggaca ttgtcggcaa acgtgatttg
    11221 tgggtggcat cttcctgctc gttgctgcac agccccatcg acctgagcgt ggaaacgcgt
    11281 cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac aaaaatgcca tgaactagca
    11341 ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc tggcagagtg gagcgcccca
    11401 attcaggcgc gtcgtcactc tactcgtgta cataatccgg cggtagaaaa gcgtctggcg
    11461 gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg aagtgcgtgc tgaagcccag
    11521 cgtgcacgtt ttaaactgcc agcgtggccg accaccacga ttggttcctt cccgcaaacc
    11581 acggaaattc gtaccctgcg tctggatttc aaaaagggca atctcgacgc caataactac
    11641 cgcacgggca ttgcggaaca tatcaggcag gccattgttg aacaggaacg tttgggactg
    11701 gatgtgctgg tacatggcga ggccgagcgt aatgacatgg tggaatactt tggcgagcac
    11761 ctcgacgggt ttgtctttac gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg
    11821 aagccaccga ttgttattgg tgacgttagt cgcccggcac cgattaccgt ggagtgggcg
    11881 aagtatgcgc aatctctgac tgataaaccg gtgaaaggga tgctgactgg cccggtgact
    11941 attctctgct ggtcgttccc gcgtgaagat gtcagccgtg aaaccatcgc caaacagatt
    12001 gcgctggcgc tgcgtgatga agtggccgat ctggaagccg ctggaattgg catcatccag
    12061 attgacgaac cggctctgcg cgaaggttta ccgctgcgtc gtagcgactg ggatgcgtat
    12121 ctccagtggg gcgtagaggc cttccgtatc aacgccgccg tggcgaaaga tgacacacaa
    12181 atccacactc acatgtgtta ttgcgagttc aacgacatca tggattcgat tgcggcactg
    12241 gacgcagacg tcatcaccat cgaaacctcg cgttctgaca tggagttgct ggagtcgttc
    12301 gaagagtttg attatccgaa tgaaatcggt ccaggcgtct atgacattca ctcgccaaac
    12361 gtaccgagcg tggaatggat tgaagccttg ctgaagaaag cggcaaaacg cattccggca
    12421 gagcgcctgt gggttaaccc ggactgtggc ctgaaaacgc gcggctggcc agaaacccgc
    12481 gcggcactgg cgaacatggt gcaggcggcg cagaatttgc gtcggggatg atgaatatta
    12541 acgagtcact gtaaattgca gtacgatgaa agaggcaaaa ctgcctgata ctaggcctac
    12601 ataatttatg caatttattg aatttttggg ttttgttggc cggacaaggc gttcacgccg
    12661 catccggcat gaacaacgcg cactttgcca ataatctgat ggcttccatc accggaagcc
    12721 attttgctat ctgcgcccgt tcagatacag aaataaccga atcattttta cgcaaaatat
    12781 aatcctgagc gatttgttca gtaatcaacg ctacgttatt tattctcact tttcacgaaa
    12841 ttgtatccct tttcagtaac gtggtagtga ggcatagact tatgtccgct atcctcaata
    12901 ataaccaaac cctcgttagc taatttgtta acaatgttaa caacattacc gaatcctgga
    12961 atttgcctta tggatagtgt gcgatcgaga ttcatccagt tccagtttat atctttctca
    13021 gccataattt tcagtacgtt aatttcagtt tcagttatca tagtcaagtg catcctggtg
    13081 gtataagtca ttaagttctc ccttaagttt ataatcttct agagtagcag tatgcgta
//
LOCUS       DAZOZD010000112        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOZD010000112 DAZOZD010000000
VERSION     DAZOZD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.112"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUPB_002583"
     CDS             <1..191
                     /locus_tag="ACHUPB_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759363.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUPB_002584"
     CDS             191..1393
                     /locus_tag="ACHUPB_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR0759364.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUPB_002585"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUPB_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0759365.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUPB_002586"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUPB_002586"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0759366.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUPB_002587"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUPB_002587"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0759367.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUPB_002588"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUPB_002588"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0759368.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUPB_002589"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUPB_002589"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0759369.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUPB_002590"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUPB_002590"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0759370.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUPB_002591"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUPB_002591"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0759371.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZOZD010000113        12937 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOZD010000113 DAZOZD010000000
VERSION     DAZOZD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12937
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.113"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            17..334
                     /locus_tag="ACHUPB_002592"
     CDS             17..334
                     /locus_tag="ACHUPB_002592"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR0759372.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            403..732
                     /gene="yjdP"
                     /locus_tag="ACHUPB_002593"
     CDS             403..732
                     /gene="yjdP"
                     /locus_tag="ACHUPB_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR0759373.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(879..1637)
                     /gene="phnP"
                     /locus_tag="ACHUPB_002594"
     CDS             complement(879..1637)
                     /gene="phnP"
                     /locus_tag="ACHUPB_002594"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR0759374.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(1639..2073)
                     /gene="phnO"
                     /locus_tag="ACHUPB_002595"
     CDS             complement(1639..2073)
                     /gene="phnO"
                     /locus_tag="ACHUPB_002595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR0759375.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(2060..2617)
                     /gene="phnN"
                     /locus_tag="ACHUPB_002596"
     CDS             complement(2060..2617)
                     /gene="phnN"
                     /locus_tag="ACHUPB_002596"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR0759376.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(2617..3753)
                     /gene="phnM"
                     /locus_tag="ACHUPB_002597"
     CDS             complement(2617..3753)
                     /gene="phnM"
                     /locus_tag="ACHUPB_002597"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR0759377.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(3750..4430)
                     /gene="phnL"
                     /locus_tag="ACHUPB_002598"
     CDS             complement(3750..4430)
                     /gene="phnL"
                     /locus_tag="ACHUPB_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR0759378.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(4541..5299)
                     /gene="phnK"
                     /locus_tag="ACHUPB_002599"
     CDS             complement(4541..5299)
                     /gene="phnK"
                     /locus_tag="ACHUPB_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR0759379.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(5296..6141)
                     /gene="phnJ"
                     /locus_tag="ACHUPB_002600"
     CDS             complement(5296..6141)
                     /gene="phnJ"
                     /locus_tag="ACHUPB_002600"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR0759380.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(6134..7198)
                     /gene="phnI"
                     /locus_tag="ACHUPB_002601"
     CDS             complement(6134..7198)
                     /gene="phnI"
                     /locus_tag="ACHUPB_002601"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR0759381.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(7198..7782)
                     /gene="phnH"
                     /locus_tag="ACHUPB_002602"
     CDS             complement(7198..7782)
                     /gene="phnH"
                     /locus_tag="ACHUPB_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR0759382.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(7779..8231)
                     /gene="phnG"
                     /locus_tag="ACHUPB_002603"
     CDS             complement(7779..8231)
                     /gene="phnG"
                     /locus_tag="ACHUPB_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR0759383.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(8232..8957)
                     /gene="phnF"
                     /locus_tag="ACHUPB_002604"
     CDS             complement(8232..8957)
                     /gene="phnF"
                     /locus_tag="ACHUPB_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR0759384.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(8978..9757)
                     /gene="phnE"
                     /locus_tag="ACHUPB_002605"
     CDS             complement(8978..9757)
                     /gene="phnE"
                     /locus_tag="ACHUPB_002605"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR0759385.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(9883..10899)
                     /gene="phnD"
                     /locus_tag="ACHUPB_002606"
     CDS             complement(9883..10899)
                     /gene="phnD"
                     /locus_tag="ACHUPB_002606"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0759386.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(10924..11712)
                     /gene="phnC"
                     /locus_tag="ACHUPB_002607"
     CDS             complement(10924..11712)
                     /gene="phnC"
                     /locus_tag="ACHUPB_002607"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0759387.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(11845..12288)
                     /gene="yjdN"
                     /locus_tag="ACHUPB_002608"
                     /pseudo
     CDS             complement(11845..12288)
                     /gene="yjdN"
                     /locus_tag="ACHUPB_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            complement(12446..12781)
                     /locus_tag="ACHUPB_002609"
     CDS             complement(12446..12781)
                     /locus_tag="ACHUPB_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0759388.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         2571 a         3654 c         3903 g         2809 t
ORIGIN      
        1 tgagcacaat ttatcaatgc aaccggtgtt tctttttaaa aaacgtaccg gagcaatggg
       61 cggtcatgtc gctggcaccg caggattctg cgttgctgtg ccagcctttt attcatggtc
      121 aatgagtgag gtgattgata aagacaccag gtcgtcagag cgcactgatt atccgcatta
      181 tgccagtgaa gttgcactgg cttttggttc acgagtggtt ggcctcgaac tggcaaaaat
      241 gattgtggat gcgtggctgg gcgcacagta cgaaggcggt cgtcatcaac aacgcgtgga
      301 ggcgattacg gcaatagagc agcggagaaa ttgagattca tccactactt gcatggatga
      361 gtaatgacta atgtggatag agtttctttt tgaggttggc taatgaaacg ctttccgcta
      421 tttcttttat tcaccctgct cacgctgtcc accgttccgg cccaggctga tattatcgac
      481 gatactattg gtaatattca gcaggcgatt aatgacgctt ataaccctga ccgtgggcga
      541 gactatgaag attcgcgcga cgacggctgg cagcgtgaag tgagcgacga tcggcgcaga
      601 caatatgacg accgccgccg ccagtttgaa gatcgccgca ggcagctgga cgatcgccag
      661 cgccagcttg atcaggagcg tcggcaactg gaggatgaag agcggagaat ggaagatgag
      721 tatgggcgat gaggttgggt atggagcagg catcgcccct caccccaatg gggagaggga
      781 gaaaacgagc gcaatattca atattgagca caatcggtcc cctcgcccct ctggggagag
      841 ggttagggtg agggggccag cgcgcacatt ccctctcatc acgccacccc aatctccatc
      901 ccatcaaacc ccacctcaaa ccctgacggt agtgcgtttt ccatcagcca cgcatcaaac
      961 tggtggctga tatgggtcag aatcacccgt ggcgagcgga taacctgatt cagcgcaagc
     1021 acggtattta aatcacagtg attacgcggt gcatccgcgc gcggcgggtg actgcaatcc
     1081 atcaccatta cctgcggctg attattgcgt aaaaatttca gcgttttttc cggcaagcca
     1141 gcggtgtcag acagccacgc cacccggctg tgcgccgttt ccagtaaata gccgaaagtg
     1201 agttttgagt ggttaagcgg cagcggcgtg acctgcaacc cctgcaaatc aaacaccaca
     1261 aacggttcca ccgtgtggct gaaatcaagc agcccgggat gtttaaacag atcgtcgcag
     1321 ccctgttcat ccggcgggcc gtagatcggg atcggatcgc ccacgcccca gcgcagcgga
     1381 aacagcccct ggacgtgatc catatgataa tgcgtcagca aaaactgctg gaacgatccg
     1441 ggagaccagc gatcggtgag atcgtgcagc ccggcgtcga tcagggtgat cgcctcgtta
     1501 aacttcacta cgccgctgca cggttggcgg cgatactgcg gcgagcgccg cgctcggtcg
     1561 caggccgcgc actcgcagcc ccatgccgga acgccctgtg cgccgccggt gccggtgagc
     1621 gtgagggtca gactcatgtt acagcgcctt ggtgaagcgg aagtgactct gctcgtagcc
     1681 ttctcgcaga tagaaacggt gcgcgtcgtg gcgcttcacg ttggtcgaaa gctcggtcat
     1741 ttcggccccg gcctggcggg cttcttcttc tgcccacgcc agcaacttgc taccgacgtt
     1801 caggccgcgc gcctgcggca tcactaccag ttcctgaatt tcgccgatcc agttgacatg
     1861 atgcagatga aactgcaaat gcaggccgat catgccgaca acctcgccat ccagcagcgc
     1921 cagatgatag cgcatgttcg ggtcgcgcag attggcgcta aagcccacgc gaaatgcgtg
     1981 gtggtcaaac tccgcctgtt ttagctcaca aatcagcgcg taaaccgcgt cggtatcata
     2041 ctgcgtggcc gggcgaagct cacaagcagg catggtgttt ctccttctga tgaatcagcg
     2101 tcagcagcgt gtcgaccgac tggcgcaggc tgccgtcatt gttgagcgta tggcaatcct
     2161 gtggagtata gcgggcggcg cgcgccaggc gggcgttaat ttcactggcg ttttcacggc
     2221 cacggttttc caggcgctgg cggaggatct ccggcgaaac ctgtaaacag acgggcagca
     2281 gcgccgattg atagcgcgcc cgcgcctgcg gcagatgggc gcgtgagccg ttgaccagca
     2341 cgtcgaatcc ggcgtgcagc cagaggtcga tctcgacgcc gacgccataa tacagaccgt
     2401 tagcgtgcca gcttaaggcc agtaggttct gccccgcgcg ggtaaaaaac tcctgctcgc
     2461 tcagagcgat atggttttcg cttccggcgc tggcgtcgcg cgtgatgtag cgatgcgcca
     2521 ccagtagctg agtttgttcc cgcaggcgga gttccgccag caggctatct ttcccggagc
     2581 cggacggccc cattaaccaa atcagttttc ccatcatcag aacacccttt taccctgacg
     2641 ccagacgtgg tcgatatgaa tgtgattgcc cttgcgatgc gccagcacca agtccgcgcg
     2701 tttgccctcg ccaatcaccc cgcgatcctg gagattaagc gcctgcgctg gatttttagt
     2761 caccagcttc accgcctgcg gcagcgtaaa gcggttgctc tcgtcatcgg cgacgcgaaa
     2821 tgccgcatcg agcaggctgg cggggtagta gtcggaagag aggatatcca gcaggccaag
     2881 ctgcgccagt tcactggccg ccacgttgcc ggagtgcgag ccgccgcgca caatattcgg
     2941 cgcgcccatc agcacgttca tgccatgctt gcgcgaggct tccgccgctt cgaacgtggt
     3001 gggaaattcg gcgatcacgc tgccaagctg gtgagattca gcaacgtggg cgtgggtggc
     3061 gtcatcgtgg ctggcaagcg caatttttcg cgcgcggcac agggcggcga tggattcgcg
     3121 attcggctgc gaccagcgtg cggcgagcgc cagttgctct tcttcgtact gctgcatctg
     3181 cgcatcagtg agggagtatt tgccctgata atattcgcga tacttctcgc gattggcgaa
     3241 ctggcgctgg cccggcgagt ggtccatcag cgacaccagc gtcaccggct cgcgctgcac
     3301 cagtttttca aacagcggca gcgtggtgtg atgcggcagt tcgcagcgca gatgcagacg
     3361 gtgctcggcg cggttgacgc cgcgtttctg cgtctcttcg atggcgttga tcatcttctc
     3421 cagattcacc agccgatcgc cgccgtcgcg cacgtcgcca attgccacgg catccagtac
     3481 ggtggtgatg ccgctcgcca ccatcagcgc gtcgtggctg ctcatcgccg agtgggcagg
     3541 ccagtcaact ttcgggcgcg gggtgaagaa tttatccaga ttatcggtat gcagctcaat
     3601 cagccccggc agcagccagc cgccttcgcc gtccatcgcc tccggcaggc ggctctggct
     3661 ttcggcaaag gcgcggattt cgccgtcctg cacctccagt gaaccgtgta ccacttcgtt
     3721 ttccagcacc aatttgacgt tattgataat catgaagagg cttccattgg gtgcaggcgg
     3781 tcggcaacgt cattacgtac ggcttcgtcg tggaagatgc cgacgatggc cgcgccacgg
     3841 gctttggctt cgtgaatcag ctcgactacg gcggcgctgt ttttggcgtc cagcgaggcg
     3901 gtaggttcgt cgagcagcag aatggggtag tcgacgataa agccgcgggc gatgttgacg
     3961 cgctgctgtt cgccgccgga gaaggtcgat ggcgccagat gccacaggcg ttccggcacg
     4021 ttcaggcggg tgagcagacg cgcggcttta gcggcgcagg cttcacgcgg tacgccggta
     4081 tcgagtagcg gctgcatcac cacttccagc gccgaaatac gcgggatgac gcgcagaaac
     4141 tggctcaccc agccaacggt ggttttgcgg atctccacca ctttgcgcgc tggcgcggtg
     4201 accagatcga cccattcgtc accgtgtttg atctggattt gcccttcgtc gggcagatag
     4261 ttggcgtaca gcgagcgtag cagggttgat tttccgctgc cggaatggcc gtggagcacc
     4321 acgcattcgc ccgcgttgac gatgagcgag gcgcggttga ggacgggcag gcgcacgccg
     4381 ttttgctggt gcaggatgaa ggttttactg acgttttgta cgttaatcat ttttcgcctc
     4441 gtggttcggg tgtcggatgc gacgctgacg cgtcttatcc gacctacggg gagcgcattt
     4501 gtaggccgga taaggcgttt acgccgcatc cggcaccggc tcaattctgc aaaaccgatg
     4561 acaccagcag ctgtgtatac ggatgatgcg ggtcgtcgag cacgcggtcg gttaacccac
     4621 tctccaccac ttgcccctgc ttcatcacca gcaaacggtc cgccagcagg cgggcgacgc
     4681 ctaaatcgtg ggtgacaatc actaccgcga ggttcagctc caccaccagt ccgcgcagca
     4741 ggtcgagcag gcgggcctgc accgacacat ccagcccgcc ggtcggttca tccataaaca
     4801 ccagcttcgg atgcgtcacc aggttgcggg caatctgcaa acgctgctgc ataccgccgg
     4861 aaaaggtggt cggcaggtcg tcgatacggt tggcgggaat ctccacctct tccagccact
     4921 tctgcgcggt agcgcgaata tcgccgtaat gtcgcgcccc ggtcgccatc agccgctcgc
     4981 cgatattgcc gcctgccgac acctggcggc gcaggccgtc gagcggatgc tgatgcacca
     5041 cgccccattc ggtgcgcagc aggcgacggc ggtcggcctc gctcattgca tagagggaac
     5101 ggttttcata gtgaatctcc ccctgctgcg gcgtcagacg cgcggagatc gacttcagca
     5161 gcgtggtctt cccggagccg gattccccga caatgcccag cacttccccc ggccataaat
     5221 caaaagagac atcgctaaag cctttgcccg gcgcgtaaag gtgggtcagg ttattgaccg
     5281 aaagtaacgg ttgattcatt ggcttttcgc ctcgctctgt tggcggcaat aatcggtatc
     5341 ggagcagaca aacatgcggt ttccggcgtc atccagcacc acttcatcaa gatagctgtg
     5401 ggtcgatccg cagatggcgc atggctcatc ccactgctga acggtgaacg ggtgatcgtc
     5461 gaaatcgaga ctttccacgc gggtaaacgg cggcaccgca tagatgcgct tctcgcgccc
     5521 tgcgccgaac agctgcaggg cgggcatcat gtccattttc gggttatcga atttcgggat
     5581 cggcgacggg tccatcacgt agcgcccgtt caccttcacc ggataagcat aggtggtggc
     5641 gatatgaccg aagcgggcga tatcttcata cagtttcacc tgcatcacgc cgtactcctc
     5701 cagcgcgtgc atagtgcggg tctccgtttc gcgcggctcg ataaagcgca gcggttccgg
     5761 gattggcacc tggaagataa tgatctgatc ttcggtcagc ggcgtttcgg ggatgcggtg
     5821 acgcgtctgg ataagcgtcg catcgtccgt acattcagtg gtgtttaccc cggtcacgcg
     5881 cttaaagaag ttgcgaatcg acacggcgtt ggtggtgtca tccgcaccct ggtcaatcac
     5941 cttcaacacg tcgctttcgc caatcacgct ggcggtgagc tggataccac cagttcccca
     6001 gccgtacggc atcggcatct cgcgcccgcc aaacggtacc tgataaccgg ggatcgccac
     6061 cgcttttaag atggcgcggc ggatcatgcg tttggtctgc tcgtcgaggt aggcaaagtt
     6121 gtagccgctc agattagcca tggttctgct cctgttgcag acgtttgagt agctccagtt
     6181 cggcctggaa atcgacgtag tgcgggagtt tgaggtgcga gacaaagcct gcggcttcga
     6241 cgttgtcggc atgcgccagc acgaactctt catcctgcgc cgggcctgtc gcgtgctcgc
     6301 cgtattccgg ggcttgcaac gcacggtcaa ccagcgccat tgccatcgct ttgcgctcgc
     6361 tcatgccgaa taccagcccg tagccgcgcg tgaagtgcgg cggctcatcc ggcgggtcga
     6421 taaaaccgtt gaccatttca cactcggtca tcagcagttc gccgacgttt accgcaaatc
     6481 ccagctcttc cggcacaatc gacacgtcga tgtaaccact gcggatctcg cccgcgaacg
     6541 ggtggttgcg cccgtaaccg cgctgggtgg agtaagccag cgccagcaaa tagccttcgt
     6601 cgccgcgcat taactgctgc aaacgggaag aacgtgaaca ggggtaaacc ggcggcgtgc
     6661 gggtgatgtc atccggctgt gctccgctat cctcttcaaa cttcgccagc ccctgacgcg
     6721 ccagcaggct gaaaacgtgc ggcgacggct gttgttcgct gtcggcggtg gtcagcgtcg
     6781 gcgcttcgcc gtttgccagc agagtgaaat cgagcaggcg atgggtgtag tcgtaggttg
     6841 ggccaagcag ctggccgccg ggaatatctt tataaaccgc tgaaatacgc cgttcgagac
     6901 gcatctccgt ggtgtcgagc ggctcgctta ccgccagttt cgtcagcgtg gtgcggtagg
     6961 cgcgtagcag gaaaatcgct tcgacgttat cgccgctggc ctgtttcagc gccagcgccg
     7021 ccagttcgcg gtcggcaatg ccgccttctg tcatcacgcg atccaccgcg aggttaagct
     7081 gctgttcaat ctgggcgacg ctcagttcgg gcaaatcggt atcgccccgg cgtcggctct
     7141 cttgcagggc gtgggcggcg tcgatcgcct tttcgccccc tttcacggca acgtacatca
     7201 gcacacctcc acatgagtgg ttcgcgggat agccagcagg cgctcgccgc aggtcaggat
     7261 taggtcgatg ccgagcggga acgggtgcgg gcgctcggtg agttcgtgca gaatgcactc
     7321 tggcagctgc ggagcgatca ttcgttcttc ggcaatgccc gcgccggtga gacgcaacat
     7381 gcgtccgccg ctcaggctgg cgacctgtaa aatcagcgtc gcgcccgctt ccggcgcaac
     7441 ggcggtgtcg gtggaaaggg cgttgagctg ttcgctggaa atcgcctcat ccgtcaccgc
     7501 gaaggtcgcc tgttccggct ggctgaccag cggcgcgttg gtatgaaaac gcaggctctg
     7561 attgacgata tcgttactta atggcgtggc aagccacacc ggcgtgtcgt tatcggccag
     7621 cgtcagcagc acgctggtgg tggcgatatt cagcggctgc cagccgcgtt tgagctgata
     7681 cagggcgaca atcacgcccg gctcgctcat ggcctttaac aggcgacgaa aactgtgctg
     7741 ggcatcctgc acgggaagca taaaagcggt ttccagggtc atgcgttgtc tccgcgaacc
     7801 atcgtaaaga agtcgacccg gctggcgttc acttcggcct ggcgtgcggc aatgcgtgcc
     7861 atacggtcag cgtccagcgg ggcaataagg gtttctgata agttttgaaa gtggcgagat
     7921 tgctgcatca gcgcatcaat cagcgcgcag cgttcggcgt gctgtttatc acgccccagc
     7981 acccagctgt agccgagggt gccgtcagtc aggcgtacgg cggcacgggt cagcgtggcg
     8041 tcgccggcaa aaaagcgttc gccggtaccg cccattcgcg cctgaatctg caccaggcca
     8101 gtttcggcag tgcggatcac ttcatagtcg gcggtgatgt tcagcgcgtt caggcgggct
     8161 gccagttcag ccggttggct gtgcgccagt acggacatcc agtgctggcg ggtcgcggta
     8221 tctgcgtgca ttcagtgctc catagtgaat tcaatcatgt cggcgcgcgt caggctgacg
     8281 gagtactccg ccgggctgct ttcaccgtca cggtggttaa gggtgcgcac gcacagcagc
     8341 ggcgacatat tcgggatttc aaggcgctgg cactctttgg cctgggcgcg gcgggcgctg
     8401 atccgcgtct ggctgcggcg cagcgcaatt ccggtttgct cgcgcagaaa atcgtgcagc
     8461 gaaccgctgt cgaaacgttg cagtgtcggc cagagggtga ggtccgcaaa gtagtggtcg
     8521 attaaacaga gcgcgacgcc gttgacgcga cgcagggtgc gcaaatggat aacgttctca
     8581 ccctcggtaa tccccagcgc gtcagcaacg tgaccggacg cggggcgcaa taccgacagc
     8641 agtttttcgc tggtgggatg gctgccctgg tccagcaggt tctggctaaa acgcgcctgg
     8701 gcgttgagcg ggtaatcgaa cgggcgcatc agcaccagca cgccgatgcc ctgacggcgc
     8761 tgtacccagc ctttttccac cagttggtcg atggcgcggc gcagggtgtg gcggtttacc
     8821 tcaaagcgcg ccgccagttg ctgctcggcg ggaagatagt cgccgcagcg atagtgttga
     8881 cgaagctcct gctcaagttt tgcggctatc tcctgatagc gtgttgggta gctggtcgga
     8941 tgtgtagaca agtgcataga tatcaatgcc tcacttatca gataaagtgc ttacgcaacc
     9001 gttgagagag gaaatccagc aggctgaccg tgacgatgat aagcaccatc agggcgcagg
     9061 tttgttggaa ctggaaaccg cgaatcgctt cccacagggt gacgccgatc ccgcctgcac
     9121 cgaccatgcc gacgacggtc gccgagcgga cgttggattc gaagcgatag agggagtagg
     9181 agatcagcag cggcattacc tgtggcagca cgccgtaaag gatctcttcc agcttgttgg
     9241 cgccggtggc gcgaatgcct tccaccgggc cgggttcaat cgcttccacc gcttcggaaa
     9301 gcagtttcga gagcacgccg gtggtgtgaa taaatagcgc cagcacgcca gcgaacggtc
     9361 cgagaccgac ggcgaccacg aacagcatgg cgaagaccat ttcgttaatg gcgcggcagg
     9421 cgtccatcag gcggcgaacg ggctggtaaa cccaccacgg caccaggttt tcggcgctca
     9481 tcaggccgaa ggggatggag agaaccactg ccagcgcggt gccccagacg gcgatttgca
     9541 gcgtgacggc catttcggtg aggtaatcct gccactggct gaaatcgggc gggaagaagt
     9601 cggcggcgaa ggtcgccatg ttgccgccgt ctttgatcag cgtaagcggg gccatttccg
     9661 cgccctgcca cgagacgacc aacacggcga gaacaatggc ccagctcaga agcgagaacc
     9721 agctgcgctt gggtggggcg atggtgatgg tttgcatgtt tggctccgtt taatgttttc
     9781 tccctctccc tgtgggagag ggccggggtg agggcatcag cgtgcgcatt tccccctcac
     9841 cctaaccctc tccctcaagg gagaggggac cgatcgagca cattactgca ccgctttact
     9901 caccgaactc atcgcgctta acgcgttgtt caggcggtcc aggtcatcca gctgcgcctg
     9961 aatctcggtg gttttcgcca gcttgtcctg ctcattcagt ccgttattgc tttttacgcc
    10021 ctgaatctct ttaaacagtg cgagctggcg aatcggcacc agttgcaggt cgctggaggc
    10081 gcgaaacggt gcccagccca ggcgttccag caccgctttc tcttccggcg tttttccgta
    10141 gttcataaag aagtcgtaga tcttgtcttt ggtggtttcg gagagattct tgcgccagac
    10201 gatcggatcg cctgggatca gcggcgactt ccagatcacc ttcagttctt tcagcttctc
    10261 tggcgcggag gttttcagct tgtcgaggtt ttcggtgttg ttggtggcaa catccacctg
    10321 cttgttggcg acggccagcg cgttggtttc atgcccggcg ttgacggtgc gcttaaagtc
    10381 gctggcggag atattgtttt tggcaaagac gtagtaaccg gggacgagga agccagaggt
    10441 ggagttagga tcgccattgc cgaaggtgag atctttccgc ttcgccagca gatcgttcag
    10501 gttgttgatc ggactatctt tgttgacgat caacacgctc cagtaacctg gcgatccatc
    10561 cgccgcgacc gtctgggcga agacctggcc gttggcgcga tccaccgctt ccattgccga
    10621 caggttgccg taccaggcga tatccacttt attgaagcgc attccctgga taatgcctgc
    10681 gtagtctggg gcaaagaagg cgttcacctt cacgcccagc ttcttctcca tatcctgtaa
    10741 gaatggcgtc cattgcggtt tcaggttttg ctgtgattcc gttgaaataa tgccgaaatt
    10801 cagcgccttt tcctgctctt cggcgtgtgc cgggcttaac agggtgctga ggctgaacat
    10861 gctggtgaag gccagcgagg caattatctt agcgttcatt cgttttcctc aatgatgggg
    10921 atgtcaggca gctttcgcgt tctcttcgat gcgattaatg ctgcggtaga gatggtcaaa
    10981 acgttcgtta tcaaactgtt ggctgctgcc gtcgtagaaa acgtgcccct ggcgcagggc
    11041 gacgatgcgt tcgcagtagc gcagggcgta atccacctga tgcagcgtga cgaccacggt
    11101 gatgccgtcg ttctgattga tgtcgcgcag ggtgtccatc acgatgcggg cggattccgg
    11161 gtccagcgag gcgatgggtt catcggccag aatcaccttc gcctgctgca tcagcgcgcg
    11221 ggcaatcgcc acacgctgct gctgtccgcc ggagagggtg gaaacgcgtt gatgggcaaa
    11281 atgcaccatg ccaacgcggg tcagcgcctg taacgcgcgt tgtttctgct cgcgggtaaa
    11341 ccagctaaaa caggtgcgcc agaacggcgt gctgccgagc gcgccaatca gcacgttctc
    11401 cagtacgctc aggcggttca ccaggttgaa ttgttggaag atgtagccgg tgttggcgcg
    11461 gcttttgcgg atatcgcgcg ccagacggcc ttcgcgctgg actgtgcggc ccagcagctc
    11521 gatatggctg ccggcggatt tatcgccggt aatcaaaccg cttaagtgac gtaaaagggt
    11581 ggatttgccg gaacccgacg gcccaagcag agccaccatt tcaccgtgat gaatgttcag
    11641 agcaaccgca tgcagcgcct gatgctgatt gaaggttttg gcgagcttct cgacacggat
    11701 aatcgtttgc atgatgcagc ctccctaaaa aagtggctcc atcgtggcgg attattgtga
    11761 cgatttggtt aattaaaagt gactaacaga tgaagagtta acgggaattc gatgacagtg
    11821 cgggagggcg ggcctcccgg cgggttattg cgttggttgt tgtttgacga cattaatcat
    11881 ccacggtacg ccaaatttat cggtgacttt gccaaagcca tgcgcctaga aagtttcctg
    11941 ccaggccatt tcgatttttc cgttagcggc aagattgtca aaccagcgtt ttccttcttc
    12001 gacctgttgc gaatcgagca ccagcgtaaa gccggagtag ctggcttttc ctgacggcat
    12061 ggcatcgctc atcatgatgt cgcttccggc aatgcgcaca ttggcgtggg cgatggcggt
    12121 atcgggaaat tgcattccgg aagggcagtt ctcggcgctg tcctgcgctg attttggcat
    12181 ttcgccgaag ctgattttat agagcagttc agcgcccaac gtacgttgat aataagcaat
    12241 tgcgtcggca cagttaccgg caaaagagag gtagggactt aacggcatga tggtgacctc
    12301 agttaagaga agccaattaa gtgtagttcg aaatttatac agatgagagg ctgcctgata
    12361 agacgcgcaa gcgtcgcatc aggcagtcgg catcgttgcc ggatgcggcg cgagcgcctt
    12421 atccggctta ccgatcacaa tacaatcagt tctttttcac aaactcagat ttcagtttca
    12481 tcggaccaaa accgtcgatt ttgcaatcga tgttatggtc gccttcaacc aggcggatgt
    12541 ttttcacttt ggtgccaatt ttcagcatcg aagagctacc tttcaccttc agatctttaa
    12601 tgatggtaac gctgtcgccg tcagccagca gattgccgtt agcatcttta acgatcagct
    12661 cgtcgctttc ctgtgcaggt tctgcgtcgt tccattcgta ggcacattcc gggcagatgt
    12721 acatgccgtt atcttcgtaa gtgtattcgg agttgcattt tgggcagtgt ggtaatgaca
    12781 tgtggatttc ctcaaaagtc agcagggcaa aagcgccaaa aaacggcaga ttgccgaaaa
    12841 aggccgcaat tatacacaaa atccctgagt ttgtcggggg tactgctgat tttagacgac
    12901 gatcgttttc atttgaaagg aaacgcaaaa agaaaat
//
LOCUS       DAZOZD010000114        12661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOZD010000114 DAZOZD010000000
VERSION     DAZOZD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.114"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            24..482
                     /gene="decR"
                     /locus_tag="ACHUPB_002610"
     CDS             24..482
                     /gene="decR"
                     /locus_tag="ACHUPB_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR0759389.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            512..2284
                     /locus_tag="ACHUPB_002611"
     CDS             512..2284
                     /locus_tag="ACHUPB_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0759390.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            2277..4058
                     /locus_tag="ACHUPB_002612"
     CDS             2277..4058
                     /locus_tag="ACHUPB_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0759391.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4239..4577
                     /gene="glnK"
                     /locus_tag="ACHUPB_002613"
     CDS             4239..4577
                     /gene="glnK"
                     /locus_tag="ACHUPB_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0759392.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4607..5893
                     /gene="amtB"
                     /locus_tag="ACHUPB_002614"
     CDS             4607..5893
                     /gene="amtB"
                     /locus_tag="ACHUPB_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0759393.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5942..6802)
                     /gene="tesB"
                     /locus_tag="ACHUPB_002615"
     CDS             complement(5942..6802)
                     /gene="tesB"
                     /locus_tag="ACHUPB_002615"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0759394.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            7020..7592
                     /locus_tag="ACHUPB_002616"
     CDS             7020..7592
                     /locus_tag="ACHUPB_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0759395.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7623..7934)
                     /locus_tag="ACHUPB_002617"
     CDS             complement(7623..7934)
                     /locus_tag="ACHUPB_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0759396.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            8097..8193
                     /gene="ffs"
                     /locus_tag="ACHUPB_002618"
     ncRNA           8097..8193
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUPB_002618"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8313..8666
                     /locus_tag="ACHUPB_002619"
     CDS             8313..8666
                     /locus_tag="ACHUPB_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR0759397.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8708..10256)
                     /gene="pdeB"
                     /locus_tag="ACHUPB_002620"
                     /pseudo
     CDS             complement(8708..10256)
                     /gene="pdeB"
                     /locus_tag="ACHUPB_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(10420..10890)
                     /locus_tag="ACHUPB_002621"
     CDS             complement(10420..10890)
                     /locus_tag="ACHUPB_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0759398.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(11006..11557)
                     /gene="maa"
                     /locus_tag="ACHUPB_002622"
     CDS             complement(11006..11557)
                     /gene="maa"
                     /locus_tag="ACHUPB_002622"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR0759399.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(11728..11946)
                     /locus_tag="ACHUPB_002623"
     CDS             complement(11728..11946)
                     /locus_tag="ACHUPB_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0759400.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(11972..12346)
                     /gene="tomB"
                     /locus_tag="ACHUPB_002624"
     CDS             complement(11972..12346)
                     /gene="tomB"
                     /locus_tag="ACHUPB_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0759401.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         2996 a         3053 c         3428 g         3184 t
ORIGIN      
        1 aaaaaattct ccgtggaagg gctatgttag ataaaattga ccgtaagctg ctggccttac
       61 tgcagcagga ttgcaccctc tctttgcagg cactggctga agccgttaat ctgacaacca
      121 ccccttgctg gaagcgcctg aaacggctgg aggacgacgg tatccttatc ggcaaagtcg
      181 ccctgctgga tccggaaaaa ataggcctcg gcctgaccgc ctttgtgctg ataaaaacgc
      241 aacatcacag cagcgaatgg tattgccgct ttgtcacggt ggttaccgaa atgccagaag
      301 tgctggggtt ctggcgcatg gctggtgaat acgattatct gatgcgcgtc caggttgccg
      361 acatgaaacg ctacgacgag ttttataagc gtctggtaaa cagcgtgccg gggctgtcgg
      421 acgtcacttc cagcttcgcg atggaacaga ttaaatacac cacttcttta cccatcgaat
      481 aaatatccag aatcaggtca ggacacaacg cgtgcgatta tttgctcaat taagctggta
      541 tttccgtcgg gaatggcgtc gctatctcgg ggctgtcgcc ttgcttgtca ttatcgcgat
      601 gctgcaactg gttccgccaa aagtggttgg tattgttgtc gatggcgtga cagaacaaca
      661 ctttactacc gggctgatcc tgatgtggat cgccaccatg gtgctgattg ccgttgtggt
      721 ttatctcctg cgttatgtct ggcgcgtatt gctgtttggc gcgtcttacc aactggctgt
      781 tgaactgcgt gaagattatt accgtcagct aagccggcag cacccggagt tttacctgcg
      841 tcatcgcacg ggtgatctca tggctcgtgc gaccaatgac gtcgattgcg tcgtgtttgc
      901 cgccggagaa ggggtgctga cgctggtgga ttcactggta atgggctgcg ctgtgttgat
      961 tatgatgtct acgcaaatta gctggcagtt gaccttattt gccctgttgc cgatgccagt
     1021 gatggcgatc atgattaagc gcaacggcga tgctttgcat gaacgcttta agctggcaca
     1081 ggcggcgttt tccagtctta atgaccgcac ccaggaaagc ctcaccagta tccgcatgat
     1141 caaagccttt ggtctggaag atcgccagtc ggcgttattt gccgcggatg ccgaagatac
     1201 cggcaaaaaa aacatgcggg tggcgcgtat tgatgctcgt ttcgacccga ccatctatat
     1261 cgcgattggt atggcgaact tgctggcgat tggcggcggt agttggatgg tggtgcaggg
     1321 cagtttaacg ctgggccagc tcaccagttt tatgatgtat ttaggtctga tgatttggcc
     1381 aatgctggcg ctggcatgga tgtttaacat tgtggaacgt ggtagtgctg cgtacagccg
     1441 tattcgtgcg atgctggcgg aagcgccggt ggtgaacgat ggtagcgaac ccgtgccgga
     1501 agggcgtggc gaacttgatg taaatattca ccagttcacg tatccgcaga ctgaccatcc
     1561 tgcgctggaa aacgtcaatt tcgccctgaa acccggtcag atgctgggta tctgcgggcc
     1621 gactggttcc ggcaaaagta ccctgttgtc gctcattcag cgtcatttcg acgttagcga
     1681 gggggatatt cgctttcatg atattcctct gacgaagtta caactcgata gctggcgtag
     1741 ccgcctggcg gtggtcagcc agacgccatt ccttttttct gacaccgtgg caaataacat
     1801 cgcgctgggt tgcccgaatg ccacccagca agagattgag catgtcgcgc ggttagccag
     1861 cgtacatgac gatattttgc gtttaccgca aggttacgat acagaggtgg gcgagcgcgg
     1921 cgtgatgctt tccggcgggc aaaaacagcg tatctctatt gctcgtgcgt tattagtcaa
     1981 cgcggaaatc ctcatccttg atgatgcgct ttcggcggtg gacggacgca cagagcacca
     2041 gatcctgcat aacctgcgtc agtggggaca gggaagaacg gtaatcatca gtgcccatcg
     2101 cctttcggca ctgacggaag ccagtgaaat tattgtgatg cagcacggac atatcgccca
     2161 gcgtggcaat catgatgtgc tggcgcaaca aagcggctgg tatcgcgata tgtatcgcta
     2221 tcaacaactg gaggcggcgc tcgacgacgc tccggaaaat cacgaggagg ccgtcgatgc
     2281 gtagttttag ccaactgtgg ccgactctca agcgcctgtt agcgtacggt tcaccgtggc
     2341 gtaaaccgct ggggattgcg gtcctgatga tgtgggttgc ggcggcggca gaagtcagtg
     2401 ggccgctgct tatcagctat tttatcgaca atatggtagc gaaaaataac ctgccgttga
     2461 aagtggttgc agggctggct gcggcgtatg ttgggctaca actgtttgcc gccgggctac
     2521 attacgcgca gtcgctgctg tttaatcggg cggcagtagg cgtagtgcaa cagttgcgta
     2581 ccgacgtgat ggatgctgcg ttacgccagc cattaagcga gtttgatacc caacccgtcg
     2641 ggcaggtgat ttcccgcgtc actaatgaca ctgaagtgat ccgcgatctc tacgttaccg
     2701 tagtggcaac tgtcctgcgc agtgccgcac tggtgggcgc gatgctggtg gcgatgttca
     2761 gcctcgactg gcgaatggcg ctggtggcga taatgatttt cccggtagtg ctggtggtaa
     2821 tggtgatata tcaacgttac agcacgccga ttgtccgtcg tgtgcgcgcc tatctggcgg
     2881 atatcaacga cggctttaac gaaatcatca atggcatgag cgtcatccag cagttccgtc
     2941 agcaggcgcg atttggcgaa cgtatgggtg aggccagtcg ttcacactat atggcgagga
     3001 tgcaaaccct gcgcctcgac ggttttctgc tgcgtccgct gctgagtctg ttttcatcgc
     3061 tcattctttg tggattgttg atgctgtttg gcttctccgc cagcggcacc attgaagtgg
     3121 gcgtgctgta tgcctttatc agctatcttg ggcgacttaa cgaaccctta atcgaactga
     3181 ccacgcaaca ggcgatgctg caacaggctg ttgttgctgg tgagcgcgtg tttgaactga
     3241 tggacggacc gcgccagcaa tatggcaatg atgattaccc gttacagagt ggcaccatcg
     3301 aagtcgataa cgtgtcattt gcttatcgcg atgacaatct ggtgctaaag aacattaatc
     3361 tctctgtgcc ttcgagtaac tttgtggcgc tggtcgggca taccggcagt ggtaaaagta
     3421 ccctcgccag tttattgatg ggctattacc cgctaacgga aggtgagatt cgccttgatg
     3481 gtcgtccatt aagttcgcta agtcacagcg cgctgcgcca gggcgtggca atggtgcagc
     3541 aagatccggt ggtgctggcg gataccttcc tcgccaacgt gacgctgggg cgggatatct
     3601 ccgaagaacg cgtctggcag gcgctggaaa ccgtgcaact ggcggagctg gcgcgtagca
     3661 tgagcgacgg tatttacacg ccgctgggcg agcaggggaa taatctctca gttgggcaaa
     3721 agcaactgct ggcactggcg cgcgtgctgg tcgagacgcc gcaaatcctg atccttgatg
     3781 aggcaaccgc cagcattgac tccgggactg aacaggcgat tcaacatgct ctggcggcgg
     3841 tgcgtgaaca taccacgctg gtggtgattg ctcaccgctt atcgaccatt gttgatgccg
     3901 acaccattct ggtgcttcat cgtgggcaag ccgtggagca gggcactcac cagcaactgc
     3961 tggctgccca gggacgctac tggcagatgt atcaactgca acttgcgggc gaagagctgg
     4021 cagccagcgt gcgtgaagag gaatcattga gcgcctgaat agcgcaatat ttcatcgttg
     4081 gtgcaaaaat gtaacgcact gtgcactgtc atagtgcgtt ttcattttca aacttcttaa
     4141 cttcctgctc tctttctcgt ttttcatttc tggcacaccg cttgcaatac cttcttcgtg
     4201 tagcagaacc attaccgaat tctgaccgga ggggatctat gaagctggtg accgtgataa
     4261 tcaaaccatt caagctggaa gacgttcgtg aagcgttatc ttccattggt attcagggcc
     4321 tgaccgtcac cgaagtgaaa ggtttcgggc gtcagaaagg gcatgccgag ctgtaccggg
     4381 gggcggaata cagcgtcaac ttcctgccaa aagtaaaaat tgatgtggcg attgctgatg
     4441 accaactcga tgaagtgatc gatatcgtca gtaaggcggc ttacaccgga aaaattggcg
     4501 acggcaaaat cttcgtcgct gaattgcaac gcgtcattcg tattcgtacc ggcgaagccg
     4561 acgaagcggc gctgtaatct ctggcacaca gcaacaggaa cgaaaaatga agatagcgac
     4621 gataaaaact gggcttgctt cactggcgat gctgccggga ctggtaatgg ctgcacctgc
     4681 ggtggccgat aaagccgaca atgcgtttat gatgatttgt actgcgctgg tgctgtttat
     4741 gactattccg gggattgccc tgttttacgg tgggttgatt cgcggcaaaa acgtgctgtc
     4801 gatgctgacg caggtgacgg tgacgtttgc actggtctgt attctctggg tggtttacgg
     4861 ttactcgctg gcgtttggtg agggcaacaa cttcttcggc aacattaact ggttgatgct
     4921 aaaaaacatc gaactgacgg cggtgatggg cagcatttat cagtatatcc acgtggcgtt
     4981 tcagggctcg tttgcctgca ttaccgtcgg cttgatcgtt ggggcgctgg cggaacgaat
     5041 ccgcttctca gctgtgttga ttttcgtggt ggtatggctg acgctctctt acattccgat
     5101 tgcgcatatg gtgtggggcg gtggtttgct ggcttctcac ggtgcgctgg atttcgcggg
     5161 tggcacggtg gtgcatatta acgccgcaat cgccggtctg gtgggcgcgt atctgatagg
     5221 caaacgcgtg ggcttcggca aagaggcgtt taaaccgcac aacctgccaa tggtcttcac
     5281 cggtactgcc attctctata ttggttggtt tggctttaac gccgggtcag cgggcacggc
     5341 gaatgaaatc gcggcactgg catttgtgaa tactgtggtc gcaacggcgg cggcaattct
     5401 tggctggatc ttcggtgaat gggcgctgcg tggtaagcct tctctgctgg gagcgtgttc
     5461 tggcgcgatt gccggtctgg tcggcgtgac gccagcgtgt ggctacattg gggttggcgg
     5521 cgcgttgatt atcggcgtgg tagctggtct ggcgggcttg tggggcgtta ctatgctcaa
     5581 acgcttgctg cgggtggatg atccctgcga tgtcttcggt gtgcacggcg tttgtggcat
     5641 tgtcggctgt atcatgactg ggatttttgc cgccagttcg ctgggtggcg tgggcttcgc
     5701 tgaaggtgtg acgatgggcc atcagttgct ggtacagctg gaaagcatcg ccattacgat
     5761 cgtctggtcc ggtgtggtgg catttattgg ctacaaactg gcggatctga cggttggtct
     5821 gcgtgtgccg gaagaacagg agcgagaagg gctggacgtc aacagccacg gcgagaatgc
     5881 ctataacgcg taacaagcac tgcaaaaaac agccggacgg ttttcacctc cggctatttt
     5941 tttaattgtg attacgcatc accccttcct gaacggtcga ggcaaccagc acgccgtcct
     6001 gagtataaaa ctcaccgcgc acaaagccgc gtgcgctgga cgccgaggtg ctctccacgc
     6061 tatacagcag ccattcattc aaattaaacg ggcgatgaaa ccacatggaa tggtcaatgg
     6121 tggcaatctg aatccccggt tcgagaaaac cgatgccgtg cggctgtaga gctaccggca
     6181 ggaagttaag atcagaagcg taaccgagca gatactgatg aacgcgcagg tcatccggca
     6241 cgctaccatt tgcgcggatc cacacctgac gatgtggttc tgcgacgtga cctttcagtg
     6301 ggttatgaaa ctccaccgga cggacttcca gcggacgatc gcagatgaat ttatctttca
     6361 gcactggcgg cagcaggtgc gccagcgatt gggcgatttg tgtttccgaa ggcaggccat
     6421 caggcgctgg cgcggacggc attgtttttt gatgttcgaa acccgcttca ggtgcctgga
     6481 aagaggccgt catataaaaa atcggtttgc cgttttgaat agcagcaacc cggcgggcgc
     6541 tgaagctgtt accgtcacgc agcgtttcga catcataaat aatcggcttc ttactatcgc
     6601 cagggcgaag aaagtagctg tgaaacgaat gtaccagccg ctcttcaggg acggtctctt
     6661 ttgcagcata caaggcctga cccacgacct ggccgccaaa cacctggcgt aaacctaaat
     6721 cttcactctg gccgcgaaag agtccttcct caattttttc cagatttaac aatgtcagta
     6781 aattttttag cgcctgactc atataactct ccagtaacta agctgccgca gcaagccaaa
     6841 gtgagttgag tataacgcaa atttgccact ggtccgatgg gtgcaatagt ctgaattacg
     6901 ggctaattac aggcagaaat gcgtgatgtg tgccacactt gttgacgtta ctattttgtt
     6961 aaccactctt ccggcgagga aagttagccc gctggtgcat tgataataag gagaagtgaa
     7021 tgaaactcgt gcacatggcc agttgtttag cggttgcgat tgcgttggcg gcttgcgcag
     7081 ataaaagcgc ggatattcag acgccagcac cggcggcaaa tacgtctatt tcagcaacac
     7141 aacaacctgc tatccagcaa ccgaatgtct ccggtaccgt ttggatccgt cagaaagtcg
     7201 cactgccgcc tgatgctgtg ctgaccgtga cactttctga cgcgtcgtta gccgatgcgc
     7261 cgtcaaaagt gctggcgcag aaagcggtgc gtaccgaagg taaacagtca ccattcagct
     7321 ttgttctgcc ttttaacccg gcagatgttc agccgaacgc gcgtattctg ttgagtgcgg
     7381 cgattaccgt gaatgacaaa ctggtattta ttaccgatac cgttcagccg gtgatcaaca
     7441 agggcggaac taaagccgac ctgacattgg tgccgatgca gcaaaccgcc gtgccggttc
     7501 aggccagcgg tggtgcaacg actaccgtac cttcgacttc accaactcag gtgaatccgt
     7561 cttcggcagt tccagctcct acgcaatatt aagcgacgtt aaccctctcc gcccggagag
     7621 ggttagtagt tccagcgata acgctgcaag tcgatttgcc cgcttcccga taccatcaca
     7681 ccttctgcca gtaatgcctg tcgctgacgc tgtaaatccg gtccggttag cgaaattgtg
     7741 ccgtggcgat taaccacccg gtgccagggt aaggtgctgc cttcagggag acgctttaac
     7801 acaccgccca cctggcgcgc ggcgcggggc gatcccgcca gtttcgccac atcaccgtaa
     7861 gtggtgacat agccttcggg aatagcggcg acgatttgcc agacgcgttg gggaaatgaa
     7921 tcttcttttt ccatcttttc ttcctgaggt aatttttcag cataatctgg aaaaacgccc
     7981 gagtgaagtc gcattgcgca agaaaccagc atctggcacg cgatgggttg caattagccg
     8041 gggcagcagt gataatgcgc ctgcgcgttg gttctcaacg ctctcaatgg gggctctgtt
     8101 ggttctcccg caacgctact ctgtttacca ggtcaggtcc ggaaggaagc agccaaggca
     8161 gatgacgcgt gtgccgggat gtagctggca gggcccccac ccatttctgc ctcccaccgt
     8221 ttcgtcaaaa aatcccaaca tagctaaact ttaaccacaa ctgacgtcgc aagaattgtc
     8281 tggctgcgca gtacgcttcg gaggtatgtc tgatgaagta tgttgatggt tttgtggttg
     8341 ccgttcctgc cgataaaaag gatgcctatc gggaaatggc cgctaaggct gcgccattgt
     8401 ttaaagagtt tggcgcgctt cgtattgtcg aatgctgggc cagcgatgta ccggatggca
     8461 aagtgaccga ttttcgtatg gcggtgaaag cggaagagaa tgaagaggtt gtctttagct
     8521 ggattgaata cccttcaaaa gaggtccgcg acgctgctaa tcaaaagatg atgtcggacc
     8581 cacggatgaa agagttcggc gagtccatgc cgtttgacgg caagcgaatg atctatggcg
     8641 gattcgagtc aatcatcgac gaataggtag cgtgacgggc tgtgcggcgt gccgtcagcc
     8701 caaaaattca caaatgttgc tcggcccagc gtaagaaatc ttctttcggt aatgccttgc
     8761 tgtagagcca gccctgaccg tagtgcacgc catgctggcg tagccactct tcttgtttac
     8821 tggtttcgat tccctccgct accattttca gtttcagtgt ttttgccatt tcgatgatat
     8881 gcggcgtgac atttttatat tccagcgcat caacgaaaga tttatcgatc ttcagaatgt
     8941 cgacatccag atcctgtaaa taacttaaac ttgaatatcc cgtaccaaaa tcatcaagat
     9001 aaatttcatg gcccgcctcc cggtagcgag aaattatcgg ggcgctggtt ttcggatcgg
     9061 caaactcgcg ttcagtgagt tcaagcgcga tctgtctggg attaacctga ttgattgatc
     9121 atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc aagattgatc
     9181 gaaatatgct gctgtggatg ctgacgcagc cagtcgccca tatcttcaaa ggcgcttctt
     9241 ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg aataaaacta
     9301 tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc agcaccgaca
     9361 attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc acgattttcg
     9421 atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg cagcacaaac
     9481 atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac ttgccgatgc
     9541 cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt catctccggg
     9601 aagggcagga tatcgtaaat gattccatta ttttcgatat gctcaccggg tgttttttgt
     9661 agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac gttatgggca
     9721 ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc aataaaggaa
     9781 gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta acggataatg
     9841 cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat tttacctggc
     9901 tcggggaagg tatcgggcgg actttcatgc tccagagacg aacactgggg aacgttgtta
     9961 tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg catttccatg
    10021 agatgggctt cgctacaggc agcgccttgc catctttcca gctcttgcag cgcatctttc
    10081 ccttgtgtcg ccaccttatt ggcccgaata gcgacgcggg aggaataggt atccagctct
    10141 tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc gacaggcagc
    10201 aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt tctcaccagc
    10261 atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag gctggcagga
    10321 gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca ttgtcatttg
    10381 accaagcgca gagctttacg cctggtatgt aaccctaact taagatgttg attcggcgcg
    10441 agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca ttttttgcag
    10501 ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa ccagcgcggc
    10561 aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat ccagcacgtc
    10621 gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat taagcaggat
    10681 aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca acatcaccgc
    10741 cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt tacggataaa
    10801 actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag actcaatcgt
    10861 ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct gcaactttcc
    10921 agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatt taccacaatt
    10981 ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct ggattaccgc
    11041 ccacgacaac gttgtccggg acatcttttg tgacaactgc acctgaggct accacgacgt
    11101 tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag acgttattac
    11161 cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg tcgatgggat
    11221 gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg atgcgaatag
    11281 ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattaccg agaaaaatgt
    11341 tatagccata gtcacagcga aacgttggct caatataagc ctctgtcacc tgaccgaata
    11401 gatcagcgag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga ttgtatcggt
    11461 gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc gagcgataca
    11521 actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg ttgctgaaaa
    11581 tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttcccattac atctgtgtga
    11641 taaagatcac atagggtatg tcttcatggt gaaaagtata aaattcttaa taaacagccg
    11701 gttatagctc cgaaagcgaa ttatttatta gcgaataaat ttccatactg aggaagggat
    11761 cttgtcgtat agtttattca tggtcaattc ggcgaggcgg tgatctgcgg ctgagtaaaa
    11821 taccgccagt tcattatctg ataattcgta tttatttttc tcgataacac gctccagcgt
    11881 gtcaattgtc tggcaacgac gtaaacgcat taaataatcg gttttcgtta aaggtttttc
    11941 ggacatactt ctacccatgg ttgtaataat tctaacaaga taaactcgca ggattctctt
    12001 tcgtcgcatt gacaaaacaa cggaatagtc gattacctga cttccgccat ttctgaagat
    12061 cctgcatatt aataccataa ctactgaaca acataaaggt gtcatccaga tattcgtcga
    12121 tctgctcaat gagcttattg tcttcattat acttaatttt gtaattaagt gcgaaggtct
    12181 caatatgctc aatcagttca ttcaactgga ggttgatcgc cgaggttggg tcgttaaccc
    12241 agccatgatt gctttcttca aggtttgcaa ggcagtcatg atacagggtt tcacagagaa
    12301 acttaagctg tgcgatatca tgtcttttgg gtgagtattc atccataacg cgtccccttc
    12361 ttagcggttg aactaacgga cacctttcgg gatggaaaaa acttactgac ctggacttgc
    12421 cctctcgttt gttagcttaa ctataagcca ctctttgcag gttttcatcg cattattacg
    12481 aaaaattaca attaggaagg aatcgggaca ggactcactt tttcatacta gcactaaccc
    12541 gcagcaggta aaagcagtct gctcgaactg ttcataacat ttcaggatta tcgccagatg
    12601 aaattggtga cactaatacc aggattgctc tgaatatgac gtaataaccg aggaatgaat
    12661 a
//
LOCUS       DAZOZD010000115        12511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOZD010000115 DAZOZD010000000
VERSION     DAZOZD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.115"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUPB_002625"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUPB_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0759402.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUPB_002626"
     CDS             433..621
                     /locus_tag="ACHUPB_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0759403.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUPB_002627"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUPB_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0759404.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUPB_002628"
     CDS             816..989
                     /locus_tag="ACHUPB_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0759405.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUPB_002629"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUPB_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUPB_002630"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUPB_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR0759406.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUPB_002631"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUPB_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0759407.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUPB_002632"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUPB_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0759408.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUPB_002633"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUPB_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0759409.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUPB_002634"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUPB_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUPB_002635"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUPB_002635"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0759410.1"
                     /translation="MFSRELDDEQLTQIASAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUPB_002636"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUPB_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0759411.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUPB_002637"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUPB_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0759412.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3018 a         3207 c         3273 g         3013 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt a
//
LOCUS       DAZOZD010000116        12359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOZD010000116 DAZOZD010000000
VERSION     DAZOZD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.116"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHUPB_002638"
     CDS             complement(<1..330)
                     /locus_tag="ACHUPB_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR0759413.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUPB_002639"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUPB_002639"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0759414.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUPB_002640"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUPB_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0759415.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUPB_002641"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUPB_002641"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0759416.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUPB_002642"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUPB_002642"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0759417.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUPB_002643"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUPB_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0759418.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUPB_002644"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUPB_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0759419.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUPB_002645"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUPB_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0759420.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUPB_002646"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUPB_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0759421.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUPB_002647"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUPB_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0759422.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUPB_002648"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUPB_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0759423.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUPB_002649"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUPB_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0759424.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUPB_002650"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUPB_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0759425.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAZOZD010000117        12196 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOZD010000117 DAZOZD010000000
VERSION     DAZOZD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.117"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..894)
                     /locus_tag="ACHUPB_002651"
     CDS             complement(<1..894)
                     /locus_tag="ACHUPB_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0759426.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREATLSEATFIDVNDTIAEGAATFSEHVAKLRILAGDVDVDKFLATTMSDTNNQL
                     AVQVRQKVKGLARAFRRNLILGNSETNTKAFDGIPRLMHKDQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGIPFIVND
                     FIPKNGVKVTEDSEQKTEGQEGTANIYCLHLSEENGLTGLYGGDNAGIVVENIGTVQN
                     KDAVRTRVKWYCSLANKHDKAI"
     gene            complement(920..1807)
                     /locus_tag="ACHUPB_002652"
     CDS             complement(920..1807)
                     /locus_tag="ACHUPB_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFR0759427.1"
                     /translation="MNLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTQEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENTRREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSAFLRDKVLMTPAKARVIYGSHFEVGDDGSVVGYDKP
                     AGHKDRAVLVDGEGNTLSFESAIERIVRADPEADAVMRSEAKQGAGSNSKPTHKVVQQ
                     KSKSTMDKLTSGLGKIGLK"
     gene            complement(1829..3403)
                     /locus_tag="ACHUPB_002653"
     CDS             complement(1829..3403)
                     /locus_tag="ACHUPB_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0759428.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDRGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            complement(3430..4686)
                     /locus_tag="ACHUPB_002654"
     CDS             complement(3430..4686)
                     /locus_tag="ACHUPB_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR0759429.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQVTVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMSASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            complement(4686..5318)
                     /locus_tag="ACHUPB_002655"
     CDS             complement(4686..5318)
                     /locus_tag="ACHUPB_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0759430.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            complement(5515..5781)
                     /locus_tag="ACHUPB_002656"
     CDS             complement(5515..5781)
                     /locus_tag="ACHUPB_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759431.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            complement(5791..6681)
                     /locus_tag="ACHUPB_002657"
     CDS             complement(5791..6681)
                     /locus_tag="ACHUPB_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759432.1"
                     /translation="MADIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            complement(6687..6941)
                     /locus_tag="ACHUPB_002658"
     CDS             complement(6687..6941)
                     /locus_tag="ACHUPB_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759433.1"
                     /translation="MPDLKIVELKPSKETDNNNVEVIRLLEEALQHAREGKSQSLALL
                     MINNDGSVLDCWHNGGRPYVMVGAMESLRLDFINANIERR"
     gene            complement(6934..7572)
                     /locus_tag="ACHUPB_002659"
     CDS             complement(6934..7572)
                     /locus_tag="ACHUPB_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0759434.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYA"
     gene            complement(7569..8237)
                     /locus_tag="ACHUPB_002660"
     CDS             complement(7569..8237)
                     /locus_tag="ACHUPB_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFR0759435.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            complement(8237..8917)
                     /locus_tag="ACHUPB_002661"
     CDS             complement(8237..8917)
                     /locus_tag="ACHUPB_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0759436.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            9000..10559
                     /locus_tag="ACHUPB_002662"
     CDS             9000..10559
                     /locus_tag="ACHUPB_002662"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0759437.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVEAGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAKNGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            10562..10840
                     /locus_tag="ACHUPB_002663"
     CDS             10562..10840
                     /locus_tag="ACHUPB_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759438.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQMKLKEKNIKMSKAEVINTI
                     LETMTMAEFDKVSSAVGVSAKAREKIMRIYENSNMTKEDLEQILSKLP"
     gene            11022..11615
                     /locus_tag="ACHUPB_002664"
     CDS             11022..11615
                     /locus_tag="ACHUPB_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759439.1"
                     /translation="MKRSLLLACIYLSSFSANALSDIVSDMQKTYSMYTSEDVLKYES
                     RGLIQPVWFRFSDNAEETILNAGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLEVQ
                     DGTLYCDEMATTGCQVNIRVGNEEPIVTYAKAEGNTSGKVLFIDENTSKRIVRDIQKT
                     ANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            11612..12133
                     /locus_tag="ACHUPB_002665"
     CDS             11612..12133
                     /locus_tag="ACHUPB_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0759440.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRSAKFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPLSNTNRIRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRKI
                     LIFFDWVERILPF"
BASE COUNT         3169 a         3049 c         2646 g         3332 t
ORIGIN      
        1 gatagcctta tcgtgcttat tcgccagaga gcagtaccat ttcacacgag tacgtactgc
       61 atctttattc tgtaccgtgc caatgttctc cacaacgata ccagcattgt caccgccata
      121 cagaccggta agaccatttt cttctgacaa atgcagacaa taaatgttag cagtgccttc
      181 ctggccttcc gttttctgct ctgaatcttc agtaacttta accccatttt taggaatgaa
      241 gtcgttaaca ataaatggaa taccattatg gcacagcata ggacgaccga agttctccat
      301 catgatttca gacgggccta cgtttactgt acgcagcagc gcacggtaag cacgcaggtg
      361 ctctgaacgc atcatgatgc agtcagcgcc caaatctttc accgcatcga ccagctcgtc
      421 gaacatagag aaagtcatag atgcaccagc aatgtcgatc ttctgatctt tgtgcatcag
      481 acgcggaata ccatcaaacg ctttggtatt agtttcagaa ttacccaaga tcagatttcg
      541 acggaatgca cgagccagac ctttaacttt ctggcgaacc tgaacagcca actgattgtt
      601 agtatcggac atagttgttg ctaagaattt atcaacgtca acgtcaccag ccagaatacg
      661 cagttttgca acatgttcgc tgaatgttgc tgcgccttcc gcaatagtat cgtttacatc
      721 aataaaggtc gcttcactaa gcgttgcttc gcgattataa agatatgcct tcgagttaat
      781 cttcataaaa ggcagaatcg caaacaggtc atcgcgatcg atgatagttt cgatcacacc
      841 ctgttcaagt tcattattgg atagcttttc agcttcttca cgcagtaatg gcatctatca
      901 attccctttg atttagatgt tacttaagtc caattttccc aagaccagag gtcaacttgt
      961 ccatcgtcga cttagacttc tgttggacta ccttatgggt tggtttgcta ttagaaccag
     1021 caccctgttt ggcttcgctg cgcatgactg cgtcagcctc tggatctgcc cgcacaatgc
     1081 gttcaatcgc agattcaaat gacaacgtgt ttccttcacc gtcaaccaga acagctcgat
     1141 ctttgtggcc tgccggtttg tcataaccca ctacgctacc gtcatcaccg acttcaaaat
     1201 gagaaccgta gatcacgcga gctttcgcgg gagtcattag aactttgtca cgaaggaagg
     1261 cagaattact aaatgacgcg ccaacggtca tctcaactaa ttgagatttc agtgatgcgt
     1321 tttcactttc cagtgctgca taacgttcgt cacgctgtgc cagctctgcc tggtgtgctt
     1381 cgatcatctg ttttttaaca gcatcgaact caccacgacg ctccagttca gcttgctccg
     1441 cctcacgacg tgtgttttct gcggcctgtt cagcctctaa aagctggcga gcacgcgccg
     1501 ggtcgatatc accgtactga gcaagctgat cggccaatgt gcgctctttc tctttgcgct
     1561 tcatgttctc cttcagcagt tcagcaccag ctttcttggt tttacgaagt tcggccagta
     1621 actcttcctg agtcatgcca gcgtattcgt catcgccctt cggctgctct ttttgttcac
     1681 cctgtttgtc aggatcttgt gtactctgct cattatcagc agctacaccg ccagcgcctc
     1741 cacgctcatg cgcttcagcg acatccatca gaccacgacg ggccaaaagc atttgccaca
     1801 gattcataaa aattcctttt gttacttatc actcgttctc ttgagtagat gagtccccat
     1861 tccctcgggg ttgatcttgc ccgctttctt gggctgcacc acgatcataa gtaagtactg
     1921 acttattttc aagggtattt agatcatttt ttggaggaaa attcaagaga tctttatcaa
     1981 attccttttt catcgcctca gtaatgttcg ggaaaatctt ctcaataagc atttccatct
     2041 ggtgacgacg tacagagtcc ggtgcctgaa gtaatgacag tttctcggca acagaaaatt
     2101 catcagtaag gccacgaata tcgaaacttt ctggatacgc aattaaagag tggtcttcgt
     2161 ccagatctac ccccatccat ttcgcaacca gtagcatgat ttggcgttca gccctttcca
     2221 gacgctctgc ttttgtgaca agcaagctat ttacacgctg gaagtcatac atttttgcgg
     2281 caccagatga attatcgatt ccctgtgcgt tgtcctgctt cgttcgctca ccagctacac
     2341 caactgaatg gtagatttcg ttaatcaccg tcttaatcgt agtgatgatc atctgagctt
     2401 gtttcgggtc tggtgacaga taaaacggct ggtttccacc ttcagaatcg taggtgaaga
     2461 ctcgctttgt gcccatttca agcactttag tgtggttttc atcaccaggt aaaagcgact
     2521 gtaccggtat agccaactgg ctaaatgtct gatcctgaat aatggcatca aggtttgaca
     2581 gatagtttgc aaccgcacga tcaagataag cgatatcatc gatcaacgat gggctgaaat
     2641 acggtgattc actttctcca atacaatcaa caggaaacac aggaactacg ccgagattat
     2701 gctcaccgct atcctctaaa ataacttttg cctgacggcg acctgctcca ccagcgccct
     2761 tcttcacttc ctcacggaac agataccact cgttttgtgt ccacagacga taacgttggt
     2821 attcctgacc tgtagaagta aaaggatctg cgtcatcacg agcgacttcc acaattaacg
     2881 cccacaacat attcccgtcg tcgtcccatg ccacatccag catttgctga ggtgaaatcc
     2941 agtaggcgta ggcgcgagca tctttctttt tctcgtcagc tactgactca acatcaccac
     3001 tcatcgtgct atcgacaaca acccatatgc gaccgtaaat agacgactgc aaatcaatag
     3061 cggccataaa tgagtcaata gaggcattct ggcgagtcgc acgtttccag aaattgcgga
     3121 tctgctctgg tgcctcttcg atatttctat gaatgtcttc tttaaagaga tatttgttga
     3181 tgaggtttac cacctcacga gtgtggttga agcgataagc acgttcaact cgctccttaa
     3241 actcctgatc tccctcttta aagtaacgaa agatattgtc tgtaaaccaa ccacgcccgc
     3301 cagcgtaagt gctggcgagg aagtcccaat gttctttttt cttttcgtat tccgggtggc
     3361 gtcgcgccac cagatcctta atttgtttgt cgttcaattc catttgaata accttagata
     3421 attacttacc tatcgagaac caccaagaat aacacgattt tttactggat acctacgatg
     3481 tactggatag cccaatgcgt ctgcactatg ctcaatgcca cctgtcttat ccatatctcg
     3541 tgttccaggc ttgtagatga ctttttccag tgaatcaatc aaatgcttgc acttcggatc
     3601 gatatacagc cggatatctc cagatgcgga catcagcatt cggttaacag cattaacacg
     3661 atccgcaatt ggcgggtgct ttttcgaata atcgacacgt aagaatccct tctctttgaa
     3721 tatgtctacg tcagactccc cacgagcatg ttggcgataa gcacctgccg gatctggaaa
     3781 taccgttacc tgtgatttcc agcgccagaa gcgtctctca agctcatcac aaacttctgc
     3841 cgtgttagag gaaaagagca ccaattcatc aattgcccat agctcaccat ttggctgagg
     3901 ttgcaaaatt actgaagaca tcgggtcaat gttgaagtcc tgccctaccc atataggcaa
     3961 ccgagggttg aactgaagcg gttttacatg cacgttacga tcgaacgggt agtaaacgcg
     4021 cccggacatg ttctcaaagc tcgccaggta ctcctgagcg aacgatttcg ggtccatatc
     4081 gttcttagca gcttcaattt ccgccgtagg tacaaacgga gaatcggcag ttacaaactg
     4141 ccagctcttc cactgtccct ttctttgcag ctctacgttc tggcctatag tccacagttt
     4201 gtgaaattcc gagaagcctt ttggcgtacc aatgatcagt gcaccgccgc gtgtcgatga
     4261 aagagtaggt cgtaacacct tgtaccaggt gtcagccttc atatcctgaa attcatcaag
     4321 cactacaaaa tgcaacgcta cgccgcgcag agtgtcaggc ttatcagcac ctttgagggc
     4381 gatctccgaa ccgttcttca acacgattgt catcgtggtg tcgttctttt tcctaaccca
     4441 cttacgaggc agaacttcct gtagatcgtc ccacaaaatc tggcgagcca tctggtaagt
     4501 aggagcgaca taccagactc tttgcttttt ttcctttgcc gccgcacgaa taatagtgga
     4561 gatcgacaac cgggattttc cccagcgtcg accagcacaa acaactttaa atcgatgcgg
     4621 agactggaag actttcatct gtccagaatg cagttgcaca agactgagcg aggacgggat
     4681 tgccattatt cgtcctcccc ttcacttcca tcatctgtcg catcaaattc gcttagagct
     4741 tcttcttcca acgtctcaag caattcgtca tcgatgattt caggctcgtc gtcttcctga
     4801 cgtaatttcg ccacctggga aggcgtaagc tcaccaaata ccaggttcgg aatttcttcc
     4861 tcgtcatttt ccgcatgatc catgcccaat gctttggacg aaacttcaaa gcattttgca
     4921 agagtattac tggctctctg taagctcttg agagaatcct caatcgcccc taaaggctta
     4981 ccctcacgtt tggccgtagt gacttcgacc atcaccatct gccccaacgc atacgcccag
     5041 ccgtcataac gtgtacgacg ttcttctatc ttttccgcac gggctttagc gcgaagctct
     5101 gcgtcagatt taagagactc acgaaccatc ttcccaacag agtccgcgcc tttctctaat
     5161 cctcgctttt tgaaatgtct ggagagtgtt tcacgacgaa tgccgtactc ttcctccagc
     5221 tttgagagtg tatattcgcc tgacgtccat ttggcttcag cttcggccca ctccgctggt
     5281 gtcaggcgag tttttacctc gtctttttcg accgtcatag atccctctaa aacacacaga
     5341 gcgcgtccat gcgctctaaa acaacttgtt tactgcatat gctaaccaac ttgttttctg
     5401 gggtgtttaa ttaggtctgg gcatgtctta ctaagcctgc ttctgtatat atttaataag
     5461 ttacttatta tttatatata cagaagcagg tctttaaata agctcccaga ccgattacat
     5521 caccagtaac ttcgctttgg ctcgacctaa agtggttaac ccaagagtcc ggcggtgata
     5581 gcgattgtca ctgcgttggc gcgtatgtcc tttctccacc agcccctttt ttatcagagc
     5641 gcgaattgag aactggatgc tttgcttggt tgtcttgtac ggcaaaactt caagcaattc
     5701 gtccagatca agtagatgac ctcgctcata accgagattg agcgttttga tgatgtcctt
     5761 ttgtttgtcg gttaacgtca tggcaaatcc ttatgccggt aaagcaattt ctaacggttt
     5821 atccaaaggt tgtttgtcga atgccagcag tggcagcgtg tcaggcagtc gacgaccaaa
     5881 gtcagggttt cggtacacac catacaacgg agacgtaaag ctcaggttgt gaatgtcctt
     5941 gagcagcttc acaatgctgg cctcgtccac cagactgtcg gcaatgtcct gaatcgtcgt
     6001 gccacgattc cgcccagctt ttgccaggga actgttcttg tggtagtccg ccaccagatc
     6061 acgcagtgca cggcgacgac gagactcgct cattgcgaac aactctttga cgatcgcctc
     6121 gttatcacca gggtcggaac ggaaatggcg ctggaataca cgcagtgcac tttcatagct
     6181 cttcggtcgc tcagggcgga tgaacttaaa ccctgctttc atggcgaagg gattgtattt
     6241 gctcatcgag gactggatct caatgattgg ccggtcatgc atcctgctaa ccaggttaat
     6301 cattcgataa gagacgccta cgccacgata ctgagtatcc acaactgagc gactgatcac
     6361 cgcaaagttg ttattcacgt accgacccca gtactggtta gctacggtgg tattagtggt
     6421 tggtttcaac ttaggaaaca tgcggtggcg aggtgccaac agtagtttcg ggtaagccat
     6481 aaccacgacg cccaccagac ggtcatcaag ttcgcagcga tagtaagttg gcgcgaaagg
     6541 tttgccgtct gttttgtagt gaagcgactt aagagcgtgc cagtcctcta cagttccctt
     6601 tgtgacagtc attcgctcca gaaagtccag atgacgcgga aactcttcag ggcggtagcg
     6661 tttgatgatg atatctgcca tgtcgatcac ctgcgctcga tattggcatt gatgaaatcc
     6721 aggcgaagag actccatcgc ccctaccatg acatacgggc gcccgccgtt atgccagcaa
     6781 tcaagaacac tgccgtcgtt gttgatcatc aacagcgcca ggctctggct ttttccttct
     6841 ctggcgtgct gaagtgcttc ttccagcagg cggatgactt ccacgttgtt gttatcagtc
     6901 tctttcgatg gcttcagctc tacgatcttc aaatcaggca tattccacct tcacgcgttc
     6961 tttgtagtgc ttggtgatct gcatatccgg gcgcagcgcg ttcttcaggt cttcgtgagt
     7021 cgtcgccacc attaccgtcg caccaacctt tcgagcggca cgctggaggt tagacgccac
     7081 aacctgagcg gttacacggt ctagaacagc gccgaactcg tcagcagccc atactttagc
     7141 gcctgactca atcagtttgg caatcttgag acgatatttc tggccgtctg acatttcaga
     7201 aggcttgcga acaaacagat atgcatcgtt cagaccagcc atagataaca acccaagcgc
     7261 atcactggtc gttttgccca actgatcgat gacgttaacc tcattatcga aggtaaaatc
     7321 atcgatggag gctacagaca gcccttcatc cttcatctgt cgttgcaact cgcgcagcac
     7381 aacggatttg ccggaaccgg attggccggt gatgtacacc acatcgccct gcttcacttc
     7441 cagctccaga ttgtcgtaaa gcgtccactc tttttcgtcc aggccaagcc cgaacgactc
     7501 agcgatttcc aacgtgcgcg tggttttatt tacgcgtgtc tgaaacgata cgttgatgat
     7561 gtatttgctc atgcagccat ctccccggaa gagattttct ccgcatatgc cacaaatgcg
     7621 tctaccccgc tttctcccgt catttcttcc atgtgggcaa gcaaatcacc aacaacaatg
     7681 gcagagccag caggaagcgt tttaaagccc aatacgtcga caacacgtac ttcttccgct
     7741 gcaacttcac gactgatctc ggtgtgttca tccttctgtc gtttagtttc ttcgccaaga
     7801 tcgataacta gcgagtcggt gtccatttct tctgtcatac tgccaacgag aacattcaac
     7861 tcacgctctt cgaagccgaa aacctcgata tcgtccagaa caagcgactc aagctctttc
     7921 tgtagcttaa ttgcatcgta atcaatgctg gcaagtcggt tatcttcaag gcgcttcgca
     7981 cgaacctcgt catcactgag atcatcgcga acaataaccg gtacacgctc aagtccagca
     8041 aaaattgcag cctcacgacg gccgtggcca gtaataatta cgtcgttctt atcgaccgta
     8101 attggctggt caaatccgcg ctttttaatg gctgcggcca gatctctgat ctgctgttca
     8161 tcatgctttt tggcattcat ctcataggga ataagatctg ccgggtcgcg atatacgatt
     8221 tcaaactttt tggtcattac atacgctcct tgtagtagtc gaccagccac accagagcct
     8281 caccagcgtt ctccatttca ttaccggtgt taatagcctg ctctttgatg atgtttttta
     8341 tggtttctgc aacacgatct gacgcatcga aagttacttt gaagcgcatg gtctgatgtt
     8401 ccgcccccac acgttcggtt ttctctcgtt tgtcggtatc gacaggctca tcactaccac
     8461 gagacaacgc ctccagtgct tcaaggtcga ttgccgcctc ttttgctaaa accatcgaga
     8521 tttcgtcgtc atacggggcg atttcagaca gttgatagtc aagttctgac tgaatttctt
     8581 caatgaagcg ttgcaatgcg atttggtcgt cttcaccgta tcgctcgttg tccaccagtg
     8641 acatctgttt agctacgaca tcgctaattt tgcccacaga aagcactgga accgttgaaa
     8701 ttccttgctc aatagcggca cgccagcgat gttcgccgcc gaggatttca aaaaatccat
     8761 cttcaagttc acgagccaaa attggcttaa aaaagcccaa tttttcgata gaacctttca
     8821 gtttttcgaa attctgcgca ccaaccgaat tggtgttcca ggtattcggg cgaaggttgg
     8881 caacatcaac ctgcaaaatc gtgattttta cgtccatttt attgctacaa tccactaagt
     8941 aatcacttac ttattataat agccaaataa catacaaaag gcactaagga aagaggttta
     9001 tgactgttcg gattgtatct aacgcagtaa atgcgcttat ttctggcgca gatgacaagg
     9061 taaagcaact ggtgcagcaa atgttgagct acgaagtcga ggctggcgac tggaagggca
     9121 caagcacaat gttcaactgg agcaaaaact cgttccctgc tggctttgcc aagcccgtgg
     9181 cggctaattt gaacaaggcg ggcatcaaat gtgttcatat ccgcaaagac aaagccccgg
     9241 cgcttggtaa accaaatccg gcggttaacc cattcccata caatcctgat tatgcgtatc
     9301 aggatcagac tgtggaaaca ctggttcgag agggaatgat gattgcgcag atcgctactg
     9361 gtggcgggaa atctaacgtt gcctgcaaag cagctgcacg tatcggtcga atgacattat
     9421 ttttaacaac ccgctctgtt ctgatgtttc aaatggccga aaacttccag agatccatcg
     9481 actaccgcgc caaaaatggc gaaccgtggt taaaagacca aaaggttgga gtcattggct
     9541 cgggtgagtt ccaggtatca cgacatatca acgtcgctac agttcaaact cttgcaagtt
     9601 tcctcgaaga accaccacgc gatgcaacac cagataagaa aagctaccac ctcaaacgtc
     9661 gggagttggt gaaacgcttc ctttcaagtg tctctcttct tattctggaa gaggcgcatg
     9721 agtcttcagg ctcaaatttc tatgacatcg ccagattatg tgtgaacgca gactatcgtc
     9781 tggcgcttac agccacgccg ttcatgaagg attcgacgga agccaacatg cgcctgatgg
     9841 cggtggccgg gcgaattgaa attaaagtca cagaaaagta tctgattgat cgaggcattc
     9901 tggcaaagcc gtacttcctt tatcataaag ttgcctacaa gccagacgag gccagaatca
     9961 aggccgaact tgccaacaaa cacctcaatt ttagagttgg tatgagcacc gcctaccaaa
    10021 aggcttatca gttggggatc gtgtataatt tgggacgtaa cgaggccatt gtgcgcgaag
    10081 cattgctcta taagcaacat tctctcaatt gtatgactct ggttcgcctt aaacgccacg
    10141 ggcaaatcct gatggaaatg atgaaagagt ccggccttag agttgacttc atctatgggg
    10201 aatctaacca ggcgacaagg caagcaaagc tgaacagttt agcgtctgga gaaatagatg
    10261 ttttaatagg ctcgactatt ctggatgtcg gtgttgatgt gccaagcgtt ggtgcggtca
    10321 ttcttgctgg tggtgggaaa gcagaagttg aaatgaggca gcgtgtcggt cgtggcttac
    10381 gagccaagaa gaatcaggca aacgtgtgtt ttatcactga tttcattgac attagcaaca
    10441 aatacctgtt gtctcactct tatgagcgaa aacacatcat cgacaccaca cctggctttg
    10501 cagaaggtgt attgcctatt gatggcgcat tcgattttgg agttctgaaa cgagattagt
    10561 tatgagcgaa aagaaaacaa cttattgtca ggtggcattg tccgataagg ccaatgacaa
    10621 acttggaaag tttcaaatga aactaaaaga aaaaaacatc aaaatgtcta aggctgaagt
    10681 tatcaatacc attctggaga cgatgacgat ggctgaattt gacaaagttt catctgccgt
    10741 tggagtctct gctaaggctc gtgagaaaat catgcgcatc tatgagaact ctaatatgac
    10801 aaaggaagat ttagagcaaa ttctaagcaa actgccgtaa tattggattc ttagtcaatt
    10861 tcagaattgc cagacttgaa gagctaatag acagagtgcg gcaacaacgc ctttgccgca
    10921 cagcttttag agaaaacacg tctgctattt acagttgaaa acttatcgtt tgagactatt
    10981 ggatatttat aattctactt tgcaataatc gctgtataaa aatgaaacgt tcgttacttc
    11041 ttgcttgtat atacctctcg tcattttccg ccaatgcgtt gtcagatatc gtctccgaca
    11101 tgcaaaaaac gtacagcatg tatacttctg aagacgtact aaaatacgaa tcacgaggtc
    11161 tgattcaacc agtctggttc agattctcag ataacgcaga agaaacaata cttaatgcag
    11221 gaaaagaaag attacggttg caaattaacg gaaccgctgt accagaaaac gttaatgtaa
    11281 gctctgacga agccatgaag ccatctggaa ttgttttgga agttcaagac ggcacacttt
    11341 actgtgatga aatggctaca acaggttgcc aagtaaacat tagggttggc aatgaagagc
    11401 ctatcgttac ttacgccaag gctgaaggga atacaagtgg aaaggtattg tttatcgatg
    11461 aaaatacctc aaaacgtatc gttcgtgaca tacagaaaac agcgaactct aaatccgtcg
    11521 ataaaaaaat ctatgttgag gttcccttct ttgtcgttgg taacaaagaa tttaaatttg
    11581 ggttgctgaa aacacctcta agagtatcag aatgagaaca ttggacatca cctacggcat
    11641 accggctgaa gtatggccgc gagactacga acatgctgaa cgtaccttgc tcttctggcg
    11701 gagtgcaaaa tttcctgtaa gagtcactct cgaagacggc caagtgtttt gcatgtatgt
    11761 ctacgggcta ttgagcgcaa gaaataagct gaacctcaca ccagatccgc ttagcaacac
    11821 aaatcgtata aggctccctc ttgagcgcat tagcacaatt gagtcgggca tagaagaggt
    11881 tgacacttca ttcacaggtc gactaacgct aaacaatgaa cagatggtta atcagccatc
    11941 acgtcgtgac ttttttaaga tatgtcgtca ggcttaccaa aacggcaaat ctataagggt
    12001 ctacatggca gacggtcgcg aaattgaggg aacgtcagat ggcgttgacg cctgtctagt
    12061 cacacttaag ttggaagacg gtagaaaaat acttattttc ttcgattggg ttgaacgaat
    12121 ccttccgttt taatttctat aagggtaata accggctaag tccggttatt attttacatg
    12181 ccattcattt tatact
//
LOCUS       DAZOZD010000118        12112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOZD010000118 DAZOZD010000000
VERSION     DAZOZD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.118"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            2..634
                     /gene="excA"
                     /locus_tag="ACHUPB_002666"
     CDS             2..634
                     /gene="excA"
                     /locus_tag="ACHUPB_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFR0759441.1"
                     /translation="MNRYPAWWTLYYVLRFIYFISIPVLSIFILFGVLSITSSRYVTQ
                     EDYIYTCVCLFLLIAPAILMYSRASSRKDKIKKIVAEIKNTGFYSPDKEYEGLSFTQG
                     VYFGVDTKKGTMLYARAYPGNIMDIIGFDIDNFTRTVTDAKTLEIYTKYINIPMVSIP
                     SGCIHPKMMADTMHAMAERGYDYPVDFPRLIQEKRKEWEQIAGMPVAEVF"
     gene            complement(706..915)
                     /locus_tag="ACHUPB_002667"
     CDS             complement(706..915)
                     /locus_tag="ACHUPB_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFR0759442.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            1307..1483
                     /locus_tag="ACHUPB_002668"
     CDS             1307..1483
                     /locus_tag="ACHUPB_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759443.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(1548..1643)
                     /gene="dqlB"
                     /locus_tag="ACHUPB_002669"
     CDS             complement(1548..1643)
                     /gene="dqlB"
                     /locus_tag="ACHUPB_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFR0759444.1"
                     /translation="MINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            2144..2395
                     /locus_tag="ACHUPB_002670"
     CDS             2144..2395
                     /locus_tag="ACHUPB_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759445.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(2467..2619)
                     /locus_tag="ACHUPB_002671"
     CDS             complement(2467..2619)
                     /locus_tag="ACHUPB_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0759446.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            2911..4119
                     /gene="trbA"
                     /locus_tag="ACHUPB_002672"
     CDS             2911..4119
                     /gene="trbA"
                     /locus_tag="ACHUPB_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFR0759447.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            4138..5208
                     /gene="trbB"
                     /locus_tag="ACHUPB_002673"
     CDS             4138..5208
                     /gene="trbB"
                     /locus_tag="ACHUPB_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HFR0759448.1"
                     /translation="MTIEYFATRIAKHISVQAIWPNGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            5201..7492
                     /gene="trbC"
                     /locus_tag="ACHUPB_002674"
     CDS             5201..7492
                     /gene="trbC"
                     /locus_tag="ACHUPB_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFR0759449.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTAQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(7529..10228)
                     /gene="nikB"
                     /locus_tag="ACHUPB_002675"
     CDS             complement(7529..10228)
                     /gene="nikB"
                     /locus_tag="ACHUPB_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HFR0759450.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRNERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(10239..10571)
                     /gene="mobA"
                     /locus_tag="ACHUPB_002676"
     CDS             complement(10239..10571)
                     /gene="mobA"
                     /locus_tag="ACHUPB_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0759451.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            10804..11139
                     /locus_tag="ACHUPB_002677"
     CDS             10804..11139
                     /locus_tag="ACHUPB_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFR0759452.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(11226..12074)
                     /locus_tag="ACHUPB_002678"
     CDS             complement(11226..12074)
                     /locus_tag="ACHUPB_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0759453.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRYLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
BASE COUNT         3203 a         3100 c         2807 g         3002 t
ORIGIN      
        1 aatcaataga tatcctgctt ggtggacatt atattacgtc cttagattta tatatttcat
       61 aagcatacca gtgttatcca tttttattct tttcggagtg ctctccatta catcttcaag
      121 atatgtgacg caagaagatt atatttatac ttgcgtctgt ctgtttttac taattgcccc
      181 tgcaatattg atgtattccc gagcaagctc aagaaaagac aaaataaaaa aaattgtcgc
      241 ggagataaaa aacaccggtt tttattcacc tgacaaagaa tatgagggac tgtcttttac
      301 tcagggcgtc tattttggcg tggataccaa aaaaggcact atgctgtatg ctcgtgctta
      361 ccctggaaat ataatggata ttatcggctt tgacattgac aacttcacac gcactgtgac
      421 cgacgccaag acactagaga tttatacaaa atacataaat ataccaatgg tctcaattcc
      481 gtcaggatgc atccatccca aaatgatggc ggatacaatg catgcaatgg cagaaagagg
      541 atatgattac cctgtcgact ttcctcgcct aattcaggag aagcgtaagg agtgggagca
      601 gattgcaggc atgcctgtag ccgaagtatt ttaagataag ctccctccat aacagccggt
      661 tcaaatgagc cggcttttgt tcattacaac agataactta tgccgttacc gataaattcg
      721 accaagtgct ttaatcgcag caatttttat ttccatatca tcactttgac tcaatctatg
      781 taattcacga gtaatgttct ccgcctgaca ccggccttca cctaatgcat agatcgcagc
      841 aagtgtaacg tcttttgatt tactcttcat catttcaaac aagaaagtat tagctatcgc
      901 aggaggcatg ctcataatca atcctcttca tgaaataaac aatggcactc acttacaaac
      961 aggagtccac tatcgcaagc gactcaactt agcaatatct ctcaatttga atgaaaaaaa
     1021 cagatggcat gtgacaggga accgtccgca tgatgtccgt gaccatttaa ccccataagc
     1081 taaccttgtc acaaacgggc gcagaacagc ctgccagaca gacaacaaaa caaaagtcca
     1141 agataaaaca tgaataccgg tatctcaatg cgaagaaata atctccgtag aaacccccat
     1201 gagaaaaata tcatatggga gaaaatcgca attatcacag ccagcgcatt cattattttc
     1261 gctgtgctct ggcattaatt attttcaaca caaaaaggaa aaagcaatga atctggtaga
     1321 cgcctttgtc aaaaaagtaa tatccgggcc atataaagaa tatggaaaat ggtggataga
     1381 tgttgaatac atttcatggg gcgttcccgg gaaaacaaga ctgatgtttg agtcaaagga
     1441 gcaggcactg gaagttaaag aagggtacaa atttttaacg tgagatctct ttttgttaaa
     1501 gggcaggaag cggtaactcc ctgcccttag ttacgtttcc aggcttatca gtcgataagt
     1561 tcccggatca gttccagaac agcaatcagc gcgtttgcaa cggcaactac tgccctgaga
     1621 acgatgatta acagattaat catccgcttc tccatcactg gagaaagaac cccgttgttc
     1681 gctcttacac tgcctgtcac aacggtttct atagtgtcag ttatagacag ttcttctccg
     1741 accagagcac caaaaccagc accacctgat gttttggcca ggacttgtaa cctgttcttc
     1801 ccgctgaaca atatgccgga cagaacatgc ctcagagctg acatgccgag tatacactta
     1861 tccagtactt gcagaaatat aattccagac gtactatcta caggtcagtt atagacagtt
     1921 cttttccgac cagagccacc aactctgtag cggaacagta aaaaagccga cctcaccagt
     1981 cggttttttt acgtctgttg tttctcaaaa tcctcccgcc atgcccgtca caccggagaa
     2041 actcatgctc tgaatcagaa ccaaccatct caactttgcc gtctcctgaa aaaactggca
     2101 cattcagttc gataactacc cataaataca gagagaaaat ccgatgtcag aaaagtggag
     2161 taaagaattc gactcttgct ttcaggtggg gagcatagaa gaaattgccg atgctctcga
     2221 gaagcgcagc ggaagcgagg aaaacgcgtt agccatgctg gcaatgacag ccttcacact
     2281 catgacccga cgtggtatct gcgaaatgca gaacgtggcc cccgatggca aaggcattcg
     2341 gctggagcta atcggcttca gggaaaatga aacaataccg gatacgcttc attgaagaag
     2401 aaagggcgca atgaggtcag ccttcgcaac aaagcggcgg cagaaaacct gccgccgtgt
     2461 tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca agcactgtgt ttccctgctg
     2521 gagccggagt ccgcaaagcg aatccctcac catccagaca aaacacagaa tcgtcacaca
     2581 gatgacgatt aacatcatta aaaacgttcg ctgtggcatc atagcccctc cattgcctta
     2641 cggcggttat cgggctacta taatgttgtc gaagcattac agtagagccc ttggttgatt
     2701 gataaaaaaa tctccagggg ctttctgctg tataccgtcc acaaaacgct ttcggcatac
     2761 agcctcaagc gcccgcagct attctaacga ctttcgtatc tgcaacaatc cttttatcgc
     2821 acggctaatt cctctggtta gctcttatcg cctgcaaata aatttttaac ctttccgttt
     2881 gatttaaatt ccagaaatgg agactttgtc atgtcttaca atcgacagcc tgtcgcagaa
     2941 gatcctatgc agatatgggg cgctgttggt gtccttctta tcctgctgct attcgtcata
     3001 tggttattct tgccggaagt ggtttacgcc agctgcctga ttcttcatac cttatgggga
     3061 ctggtcgact gggggccatt tcataactat gcagcaccac gatacaacct gcttgccatg
     3121 accggtaaca acgccgccaa cataagctat agccagtggg ttaacgtcat ggagcagacc
     3181 ataggtattc tgtggatgta cctcttacct gtgacactct ggtgtttgtg ggaatggtat
     3241 caacatccgg gacaaagccg tttcacccgc agaccagtag atattacccg actgccacac
     3301 atctttgctt cgttgtcccc tgctatcgcc cctgtactgg cagatggtga tccggaaaaa
     3361 ctctttcatg gtggcaaacg acctgaacgg cgggttgctc tcaccccgga agcatttgtc
     3421 gagcagcata ctctcatcac caatatgcag ctcgatgttg cagctgcaag acgatgtttc
     3481 atggcccagt tgggtaaacc tttaacgtcc tggaaagaca tggcaccaca tgaaaaagcg
     3541 ctctttgcca tatttggtct tcagtatttc ctggatgatc gtaaggcagc attaaagctc
     3601 atggatacac tgaacctctc atgcagaatt aaaagcaaac gggacagtgg gaaattctgt
     3661 acccctgttt attcactggc caaatcagct ttccagagag tgattaaaag caacggagca
     3721 cagcaatggc tgaaacaaca tcgctacgtc cgcagtgggc tggtctggct gtatgcacat
     3781 gacctgcggc ttacgccacc caactggatt tggctgaaag gcgtggacag gacgctgttt
     3841 tatgcattac accgcgccaa taccacgaaa ggatttattg aaggcgctgg cgtggtggcc
     3901 gttgcacgtg cagaggccga agccatgcgt tttggtcttc cctgccctga accctgtgtt
     3961 gatgaagcgg tggaaggctt acggcgggat atgctcagtc tcggtcttat ctgggatgaa
     4021 ccacaaccag atcgcgaccg gaaacgccgc atcctgacca actggtccct caccgacgac
     4081 atcctgcccc gcacacctgc tacagacaac gaattctgat tatccggaga actgaaaatg
     4141 acaatcgagt actttgcaac acgcattgca aaacatattt ccgtgcaggc aatctggccg
     4201 aatggcagga cagaaattat tgccgtcctt ccacgggatg ccctgtcagg gctggtaaca
     4261 cgaaataatc aactgattca tatagccttt gacgccatgg gcacccgtaa cagagaaacc
     4321 gtcctgattg ataaacagga gcataacgct gaccagatac ttacagctat tgattcctgc
     4381 ctgcgccttg aatatctgat ggaacgccgc ttcagcagaa cacctttgtt ccgtggcata
     4441 atcgccagcg ttgtgctgtt cgtaatggca acgattggct tctctctgtt ccgctatgtt
     4501 gatcgtgtat tctgggatga tacaacgcct gaagcagtac agacagcagg tgagccacga
     4561 ctgcttcccc ctcacctgaa tcacactgtg ccattaaatg aaggcattca actcccggtt
     4621 cccccgaaaa aagacgtcca ggtaccggag aaaacaatca caagcaaaaa tccggaagcc
     4681 gcagctgcgc gacataatct ggctactgtt ctgaagcgga acgccgatcg gggcatgttt
     4741 accatcaacc tgtcatccgg tcatgaacga accctgttcg catttctcga tcctgcctgt
     4801 cctaactgca gacttctgga gccggcctta aaacgcctgg ccagcgactt caatgtcgtt
     4861 atctacccgg tttcagttat tggtggagag gaatccaccg accgtgtggc accactgctg
     4921 tgtgaaaaag atgctcagaa acgtgctgcc ggctggcacc gactgtattc cgcagataac
     4981 ggaatgatga cgccatcaga agaaacaacg cctgcagatg aaacatgcct gaaagcagcc
     5041 agagctgcga ttgatgttaa caatgtcgcc ttccgtaagt tcggatttgc cggtacccca
     5101 tgggtgctgt ccgacacggg ctggcatctg cctactggaa ttctgcagga aactggcacg
     5161 cttaatttat tcctgaaaac caccgacagc gagtctggcc atgagtgaac atcgcgttaa
     5221 ccccgagctt ttacaccgta cagcctgggg aaatcctgtc tggaatgcac tccagagtct
     5281 caatatctat ggcttttgcc tggtggcaag tcttgtggcc agttttatct ggccacttgc
     5341 attaccggca tgtctgttgt tcacactgat aacgatgctg gtatttagtc tgcagcgatg
     5401 gcgctgccct cttcgtatgc ccatgacgct ggaatgtgct gacccgtcac aggacagaat
     5461 gattaaaaga agtcttttca gcttctggcc aaccctgttc cagtatgagg tcattctgga
     5521 atcaccggcc agcggcatat tctatgttgg ttatcaacgc gtcagggata ttggcaggga
     5581 gttatggctg agtatggacg atctgacccg ccatataatg tttttcgcca ccaccggtgg
     5641 cggtaaaacg gaaaccatct ttgcctgggc gattaaccca ctttgctggg caagaggatt
     5701 cacccttgtc gatggcaagg cacagaatga tacggcaaga acgatctggt atctggccag
     5761 acgctttgga cgtgaagacg atgtggaagt cattaacttc atgaacggag gaaaatcccg
     5821 cagtgaaatc attctgagtg gtgaaaaaac gcgtcctcag tcaaatacct ggaatccctt
     5881 ctgttacagc actgaggcat tcacagcgga gacaatgcag tcaatgctgc cccagaacgt
     5941 ccaggggggc gagtggcagt cccgggccat tgccatgaat aaggcactgg tatttggtac
     6001 taaattctgg tgtgtgcgcg aaggcaaaac aatgtcgctg cagatgctcc gtgaacatat
     6061 gactctcgaa gggatggcca aactttactg tcgtggcctc gatgatcagt ggccggaaga
     6121 agctatcgca ccgttacgta actaccttca ggacgttccc ggtttcgacc tgtcgctggt
     6181 caggacacca tcagcctgga cggaagaacc cagaaaacaa catgcttatc tctccgggca
     6241 gttttctgaa actttcagca cattcacgga agcattcggg gatatttttg ctgaagattc
     6301 cggtgatatt gatatccgtg acagcattca cagcgacagg attctgatgg taatgatccc
     6361 cgctctggat acatcagcgc atacaacatc tgctctggga cgaatgttta tcacccagaa
     6421 aagtatgatt ctcgcccgtg atttgggcta ccgcctcgaa ggaactgatt ccgatgcact
     6481 ggaagtcaaa aaatataagg ggcgtttccc ttatctgtgt ttccttgatg aagtgggggc
     6541 atactatacg gaccgtattg ctgtggaagc cactcaggtg cgttcactgg attttgctct
     6601 gattttgatg gcacaggacc aggaacgtat agaaggacag acaacagcca cgaatacggc
     6661 aacactgatg cagaacacgg ggacaaaatt tgccggcagg attgttagcg agggtagtac
     6721 ggccagaacg ctgaaaagcg ccgccggtga agaagccaga gcgagaatga acaacctgca
     6781 gcgtcaggac ggcatatttg gtgaatcatg gattgacagc ccacaaatca gcatcctgat
     6841 ggaaagcaag ataaatgttc aggagttgat tgaacttcat cctggcgagt tcttttctat
     6901 tttccgggga gaaaccgtac catcagcctc attcttcatc cctgacgatg agaaaagctg
     6961 cagcagcgat cccgttgtta taaacaggta tatcagcgtg gatgcgccac gcctggaccg
     7021 tctccgccgt cttgttcccc gtacagctca acgtcgaatc ccgtcaccgg aaaatgtcag
     7081 tgccatcatt ggcgtcctga ctgcgaaacc ctccagaaaa agacgaaaaa ttcgcacgga
     7141 gccgcacacg atcgtggata cattccagca acgcattgca ggacgtcagg cggctatggc
     7201 aatgctggaa gagtacgaca cggatatcaa cgcacgtgaa agtgccttgt gggaaacagc
     7261 ggtgaatacg cttaaaacga ccacacggga agagcgacga attcgctata tcacgctgaa
     7321 caggccagaa ctacctgaaa caaaagagga aaatcagata tcagtgagag cagaaagagc
     7381 aggtataaac ctgttaacgc ttccacagga caataaccac ccgacagggc gtcctgtaaa
     7441 cgggtttcat cataaaaaaa ccaaccgtcc ggactgggac ggaatgtact gaacagtggc
     7501 cgcgccggat tctggcgcgg cctttttttt aacctggaga aggcggttcc cagcgtttat
     7561 cctgctccgg cggaggcagt gatgcgtcat cgttgctgtt cgccgcgata taatcgcggt
     7621 agtagcgttc acttccttca cggtgatgat ccacgtccgg tcgcgtaatc ctgtagtctt
     7681 cgtgaccggt acggcctgtc acattatgcc aggcaaccat ttcagcaaag acctggttaa
     7741 attgtttgtc gtttccttct ggttcaaaac ccatgcgtgg atcacgcccg aataataccg
     7801 gtgcaatcaa atccagttta tctgccagcg cattgcggtc atggtgatta cggaaaacaa
     7861 ccctgtcacc ataatccgta cagacaaact ctcccgtccg acgatcacgg aaacgaacac
     7921 tcccgttctt ctgcaaacgg gcctcgagat caccggtatt accgtatacc ggcgttccgc
     7981 ccggttcaca aagcacgaca cagcggtctg ttgtcgttgt gcgtgactta tctttacggc
     8041 gatcgcgata atcccagcct ctcagctgcg atacagcagc cctgtctccc tgagcagcct
     8101 gaatttcgac ccactgacgg taagagggtg gataccactt cccgtcagca ataagtttct
     8161 gccgctcatt cctgatgtct tctttcagcc tgatcaatgc ctgcattcgc tgaacttctg
     8221 caatgtgata atgcagctta cgtaatgccg gatcatcgta ctgcgcacga atgtgtgact
     8281 tccgcagacg gcatgcctga tgaatttccc gacaacgttc cccataacgc agatctggtt
     8341 tacgccattg ttcacgccat gccagatagc gggcacgcag gtcagcacgg gcctcagctc
     8401 tggcttcacg tcgctgccgg cgcagcattg ggtcacgttt actgctgacc ccgtacctgt
     8461 cactgacagc cagctccggg ttatagcgac tttcaggttg caccctgtca aacaaatctg
     8521 ctggggcact cacaaaaggt ccggcctgtg gctctgcacg ccccagagtc agatcaggat
     8581 gaatgctgct ggccttcacc ggcgtttgct catgattaaa ggcatcaacg acaacaagcc
     8641 cgtgatgttg tttctgtagc atcagtccct gttgtgcaaa caactcatga caacgctgcc
     8701 aggtaaactc accctgtgtg atcatttctt caatctgagg gctacaatgg ctgatgctgt
     8761 atccccacag actttctttg tcagaaaaca gttcgcgttt gatagccccc tgcggccccg
     8821 tacctgtacg catacggtgc cattctttat cggtcgcagg acagctctcc atacgacgac
     8881 cacggaagcg ttctgttggc gtaacctgga ggaaaatatc tgttggtaca ggctgccagc
     8941 caccatcgag ttgattcaca ttgtcgagcg tcagcagtga ccatacgctg tcagcacgaa
     9001 cgggagtctg gttatgatca taaccgtcac agataaccag gtgaccatgc tgaacacgca
     9061 tccataatcc cgcttcagcc agtgtgcggt gaatagcctg gcagttttcc agccgtcctt
     9121 cccgtgccat ttccggcaga cgttctccaa gatgacgaca ggcatactgc tgcagacttt
     9181 ctgtttcagc gattttttcc gggcgaacat cagctgcaat gaagtccgga ttatagcgtc
     9241 ctggtgcttc cacctggctg aaaatgtctt ttggtactgg cgtgtagtca cccatttttt
     9301 tcatgagttt ttctgcacac caggagggac caaacgagtc aagctgtaca ccttccctgt
     9361 tgcgatccca gccatccatc accagaaatt ttccgtccat ctgagacagg tacagaccat
     9421 cttccgcaag acgacgatgc agggataacc agtcatttac aggttcactg cgtaaaccag
     9481 cgaccgctgt ctgcgcgaca tactcgcgaa aagtttgttt ttttccgcgt gtccaagcgt
     9541 tctggcgatc acgttcaacg gcagttttgc gaacgatacg atttcccggc gcatggaccc
     9601 agcaaccgtt atccggagca aaaccatgct tcagttcaag ctcacggcag gcacgactga
     9661 gtttttcctg actccatgat aatcggttca gataacccgt ttcagggtgt acccggttaa
     9721 cagccacatg gacgtgcaga ttatctgtat cagtatgcac ggcagagaca tactgatggt
     9781 cggcaaggcc gagtgatttc agcgtatgac gtacactgtc ataaatctgt tccggacgcg
     9841 ggctttcatg tgactgccag gaaaggatgt agtgaaaaac aggatcagtg tcatcttttg
     9901 catagtgtgc ctgccgggca atgtactcca tatctgcagc ggcagtttca agagaagtac
     9961 agttatgaaa gcaggtgaca ccataaaagt tgacccattc gcagccgtcc ttcatgacat
    10021 caaccagcgc cacgaatgac tcatttcgaa gacgtgtcgc ataatcaaca aggcgactga
    10081 aacggcttcg gtgcggttgt tcagcctgcg aagaagaaga taaatccagt tcctcatcgg
    10141 tcatgtcatc ccggacagag acgtaggata ccaggtcttc gaacgaagac ttgccgtccc
    10201 ttctcttttt cggaatgact gcattcaccg gagaatcctc aaccttccat taactgttta
    10261 cgcaatgtat ttgtaagttc ggttatggca acaagcactt ctgcgtattc cttgtcaccg
    10321 gttctttttc cctgaacaaa cagatgcttc tgcatccttc ccagtctcat taactccttc
    10381 agaaaagcat catctgtccg ggagttaatt cgcttattca gtgctgcatt tcggatgtaa
    10441 gcagatacag taagtccgga atcttcagca ttcttacaga tagtttcatc ctcatccggt
    10501 gagaaccgga ccgctctcag gatcgtcttt tgccgtcttt cactaccact tctttgacgt
    10561 ttatcactca cggtcagctc cttactgggg tgtcggggcg aagccctgac caggaggcaa
    10621 ttgtataatc gcgcgtgcgc ggttatacaa ttgcacatcc tgtcccgttt ttcaggccat
    10681 tatagcctgt tatttttgtg catcatagtc ttatgcagac ggcagaggca gggcgcagcc
    10741 ctgccaggag gcattttttg atgataaaat cagacttcaa catgaaacgg agagaaagtg
    10801 ccaatggctt ctaaaaaatt ttactcagat gatgacatcc agctggcaaa agctgcttta
    10861 tcggaattgc cggacctcac cgctcaacgg aaaacattgc gtgattttct tgatgccatt
    10921 cgtgatgaca tcattatcct ggtaaggaca aagggttata cactggcaga tgttcgtgac
    10981 acgctcaaaa atgcagggta tgaagtagga gagaaggcac ttcgtgacat tattcgggaa
    11041 gcagaatcga aaaaaccatc ccgccgttct tcctctaaaa cagcacctaa aaagacagat
    11101 tcaggcagga aagatggcat cgatatgaac aacaattgat gtgacaatgt cacatcgtgt
    11161 tctgagatta tgatgattac agaaaaaaaa agccccggca taaataccag ggcttttctg
    11221 tttaatcaga ctcgtggcgg caatgcacct ggataatgcc gtgcaataat atcattcagt
    11281 ttttcaacaa ggtcagaacg cttaaagaca atgtgtacgg tccctttctg gtagccacgc
    11341 accgtaaaat attccccttc taacagctta ccgacattgt cgccgttccc gagaaattca
    11401 ccatactgcg ccccgatgct gttcctgaag tcaggaacgt tgcgcccctc cagcagataa
    11461 aacggacgta ccaggtcatc cagtgcctcc cgtccgctgc agtccagcgt cacacgccca
    11521 ttactccagc ggtacagcag attttcaata atgattttct tacccaggcg gcagggatta
    11581 ttggttctgt aatcccatga caatttccgg tagacatcaa tcagtccctg ttcaaatgtc
    11641 tgcatcttac tggcattcag atagcgaaaa gttgccagta cattgtccag tgtgatttca
    11701 ggacaggtgt cgctcatcag ctggctgttc cactcatcac gctggcatga actcatgaag
    11761 gtgtacatgc cagtatccgt catcaggcga tcccagattt tccggtcggc aagacgagtg
    11821 atattctttt ttgcttcttc cggtttatcc atgcatcgaa cggagtcagt cacgcagtct
    11881 tcaaacccgt agagaaaaat gtcaccggaa gcatcgagca gaatcctgcg cgctgtcgcc
    11941 aggcattcca tcgcctgcat gaacagtgca ataccttcgt tacgctgcgc gatgatgcgg
    12001 tcgatagcca cagacggaat aacgtctgtc gtgccggcag tattcaggtc agcagtgggt
    12061 aaagtctgat tcatcacatt gtctcctcat cgtcacgact gccgggtcag cc
//
LOCUS       DAZOZD010000119        12083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOZD010000119 DAZOZD010000000
VERSION     DAZOZD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.119"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..892)
                     /gene="galU"
                     /locus_tag="ACHUPB_002679"
     CDS             complement(<1..892)
                     /gene="galU"
                     /locus_tag="ACHUPB_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0759454.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEE"
     gene            complement(1094..2107)
                     /gene="rssB"
                     /locus_tag="ACHUPB_002680"
     CDS             complement(1094..2107)
                     /gene="rssB"
                     /locus_tag="ACHUPB_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0759455.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2199..3104)
                     /gene="rssA"
                     /locus_tag="ACHUPB_002681"
     CDS             complement(2199..3104)
                     /gene="rssA"
                     /locus_tag="ACHUPB_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0759456.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3217..3675
                     /locus_tag="ACHUPB_002682"
     CDS             3217..3675
                     /locus_tag="ACHUPB_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0759457.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3725..4567
                     /gene="purU"
                     /locus_tag="ACHUPB_002683"
     CDS             3725..4567
                     /gene="purU"
                     /locus_tag="ACHUPB_002683"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0759458.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4727..4811
                     /locus_tag="ACHUPB_002684"
     tRNA            4727..4811
                     /locus_tag="ACHUPB_002684"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4761..4763,aa:Tyr,seq:gta)
     gene            4859..4990
                     /locus_tag="ACHUPB_002685"
     ncRNA           4859..4990
                     /ncRNA_class="other"
                     /locus_tag="ACHUPB_002685"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5174..5851)
                     /gene="narI"
                     /locus_tag="ACHUPB_002686"
     CDS             complement(5174..5851)
                     /gene="narI"
                     /locus_tag="ACHUPB_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0759459.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5851..6561)
                     /gene="narJ"
                     /locus_tag="ACHUPB_002687"
     CDS             complement(5851..6561)
                     /gene="narJ"
                     /locus_tag="ACHUPB_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0759460.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6558..8096)
                     /gene="narH"
                     /locus_tag="ACHUPB_002688"
     CDS             complement(6558..8096)
                     /gene="narH"
                     /locus_tag="ACHUPB_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0759461.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8093..11836)
                     /locus_tag="ACHUPB_002689"
     CDS             complement(8093..11836)
                     /locus_tag="ACHUPB_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0759462.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2879 a         3275 c         2964 g         2965 t
ORIGIN      
        1 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
       61 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
      121 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
      181 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
      241 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
      301 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
      361 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
      421 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
      481 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
      541 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
      601 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
      661 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
      721 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
      781 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
      841 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
      901 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
      961 caccccgaag gggctaagtt ctgaaaagga ggcgagttag cataattaag acaattactt
     1021 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     1081 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     1141 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     1201 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     1261 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     1321 gcggaaactg ccccggcaga ttggcctgac ggagtaaatg gtttacctgc ttcaataacg
     1381 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     1441 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     1501 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     1561 tgtccgcggc aaccaattga cgataattaa cccggcaatg ggaaatcacc tgctgaaccg
     1621 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     1681 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     1741 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     1801 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     1861 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     1921 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     1981 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     2041 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     2101 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     2161 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     2221 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     2281 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     2341 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     2401 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     2461 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     2521 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     2581 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     2641 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     2701 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     2761 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     2821 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     2881 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     2941 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     3001 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     3061 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     3121 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     3181 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     3241 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     3301 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgcagattat
     3361 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatggcc
     3421 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     3481 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     3541 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     3601 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     3661 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     3721 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     3781 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     3841 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     3901 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     3961 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     4021 cgatttgttg atgaaagcca attacggcgg cctggatgtc gaaatcgcgg cagttattgg
     4081 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     4141 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggaagcca ttgatgctta
     4201 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     4261 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     4321 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     4381 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     4441 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     4501 tcgtgcgcta tacaaagtac tggcacagcg cgtctttgtt tacggtaatc ggacgattat
     4561 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     4621 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     4681 cccgcttccc gataagggag caggccagta aaaagcatta ccccgtggtg gggttcccga
     4741 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     4801 ccccaccacc atcacttttc aaaagtccgt aaactctcaa acgaatccgc aattaaatat
     4861 tctgcccaag cggggaagga tgagaagctt cgaccaaggt tcgacttgag cgccagcgag
     4921 agagcgttgc cgcaggcaac gacccgaagg gcgaagcgcg cagcgctgag taatccttcc
     4981 cccaccacca tcgcttcctt aaataatcaa aacctgttac tccgagctaa ttttgcccga
     5041 tggcaccacg tttaccgggc ctacaatatc ctcccctgta ggtcggataa gcgaagcgca
     5101 tccgacaact caccccgcac cgtgcccata aaaaaacccc gccgaagcag ggtctatgtg
     5161 aactaaaatt cgcttagtga cgagcgcgca ccagctggta cttacgtgtc agatactcca
     5221 ccggtacgct ccagatgtgt accagacgcg agaacgggaa cagcaggaat aacgtcatcc
     5281 ccagcaccag gtgcagacgg aagataaacg ccacaccatc gaggtgttga gaagcgccac
     5341 cgtggaaggt caccaccgac tgcgcccagc caaccagttt catcatctcg ctaccgtcca
     5401 tatgctgagc ggagaacgga atggtcaaca ggcccagcgc gcactggata acgagcagcg
     5461 acaggatcag gatatccgct ccggtagtgg ttgcacgcac gcgtgggctg aacagacgac
     5521 gcttcagcaa cagcacgccg ccaatcagac acagcacgcc gctggcacca ccagcaaaca
     5581 tcgccatttt ctgtttcact tcaatcggca gccaggcttc atacatccag tgcggcgtca
     5641 gcataccgaa gaagtgacca acaaaaatcc ccagaatacc gatatggaac aggttcgacg
     5701 ccaggttcat ccctttgcga tccagcatct ggctggacgc cgcgcgccag gtgtactgcc
     5761 cgtagtcata acgcagccag ctaccaatca ggaagaccgc cccggctatg tacgggtaga
     5821 tatcaaagaa gaacatattc aggaattgca ttagtgctgt cctccggtgg tgatattcag
     5881 atattgcggc gcgacggcac cggcaaagcg acgctgatga gcagtgattg ctgaatcacc
     5941 gcagcctttg tcagcaaaga atttaacctg ctcttcttcc cagacagcat ccagcgcctg
     6001 cggcgtatca tcgcgcgctt cgtcggcaat tttttccgcc actttgtcgc tgtcgatagc
     6061 ggtattggcc aatttcagca gcagatcaaa catcacggca taacggcttt cacgctgttg
     6121 cagacgcgcg ctcagcaatg ccagaatcgg cgcgatatct ttcaaacctt ccacggcttc
     6181 gctttgcggc agctgcgaca ggtactccag atacagcggc agatggtccg gtaattcgcg
     6241 gctgtttaac tgcaagccgt gctgctcgta ctgcgccagc aggtccacca tcgcctggcc
     6301 gcggtcacgg gattcaccgt gaacatgttc aaatagcaac agtgacgtgg cgcgaccacg
     6361 gtcgaacagt tcgctgtact gggcctgggc atcgagcgga tccatcgccg ttaaatcgcg
     6421 caggaaaatg cccagcgcat gggcatcttc tttcgacaga tttttcgacg cagcaatcgc
     6481 ctcaaacatc tcttgttgat gctgccataa ggcagcatcc ggatattcaa ggagacgcga
     6541 tacaatcacg agttcgatca tggatgcggc tccgttttgc tggtcacatc gatggcatcg
     6601 atacgacggc tgttgaacag attgaatttg gtatctgaac cgtggcagcc atcaccaaag
     6661 gtaaagccgc agccattttt ctccgggaag gcttcccgtg ccagttcacg atgactactc
     6721 ggcaccacaa agcgatcttc gtagttagca atcgccagat aacggtacat ctcctgtgcc
     6781 tgggcttcgg tcagaccgac ctcttccagc gcacgggtat caactttacc gtcaacggtt
     6841 tcagcacgtt tgtaatgacg catcgccagc atacgtttca gtgcgcgcag taccggtttg
     6901 gtatcaccgg cggtcagcag attcgccaga tactgtaccg gaatacgcaa gctttcgacg
     6961 tctggcagaa tgccgttgct acccagctca cccgcgtctg ctgcagactg aatcggagac
     7021 agaggcggca cgtaccagac catcggcagt gtgcgatatt ctggatgcag cggcagcgcc
     7081 agtttccatt ccattgccat tttataaacc ggcgactgct gtgcggcttc aataacgctc
     7141 agcggaatac cgtctttaat cgcctgctcg atgactttcg gatcgttcgg atcgaggaac
     7201 acctccagct gacgctggta aagatctttc tcgttctcgg tgctggctgc acgttcaatc
     7261 gcgtcggcat cgtacagcag cacgccaaga taacggatac gaccgacaca ggtttctgag
     7321 cacacggtcg gctgacccgc ttcaatacgc ggatagcaga agatgcactt ctcagactta
     7381 ccgctcttcc agttgaagta gatttttttg tacgggcatc cggtgatgca catacgccag
     7441 ccacggcatt tatcctggtc gatcaggacg atgccatctt cttcacgctt gtaaatcgca
     7501 ccgctcgggc aggtcgccac acatgccggg ttcaggcagt gttcgcacag gcgcggcaaa
     7561 tacatcataa aggtgttttc gaactggcta tacatcgcct tctggatgtt gtcgaagttc
     7621 ttgtctttcg ccagtttgtc aaactcacca cccaggtcat cttcccagtt cggccctttt
     7681 tcgattttcg ccatccgttc cccggtaatc agcgaacgcg gacgggcaat cggctgcgat
     7741 ttgctgcctt ccggcgcggt atgcaggttc tgatagtcaa aatcgaacgg ctcgtaataa
     7801 tcgtcgatcc ccggcagatg cgggttagcg aagattttac ccagcaacat ggcacggtta
     7861 cccatgcgcg gctgcagttt gccgttgatt ttacggatcc agccgccttt gtatttttcc
     7921 tggttttccc agtcagtcgg gaagccctga cccggcttgg tttccacgtt gttgaaccac
     7981 gcgtattcca cgccttcacg gctggtccag acgtttttac aggtaactga acaggtgtgg
     8041 cagccgatgc acttatcgag attcagcacc atgccgactt gtgaacgaat tttcatttta
     8101 cgctctcctg tacctggtca ttgccttcgc catctaacca gtcaatgttc ttcatcttac
     8161 gcactacaac aaactcatcg cggttagaac ctacggtgcc gtagtagtta aagccgtatg
     8221 ccagatgggc atagccgccg atcatatgcg tcggtttcgg cgtgatacgg gtgaccgagt
     8281 tatggatacc accacgctgt tgggtaattt ccgaaccagg caggttaacg atacgttcct
     8341 gcgcgtggta catcatggtc atccctgccg gaacacgctg gctgacaacc gcacgggcag
     8401 tcagagcacc gttgctgttg aatacttcaa tccagtcgtt atcggcgata cccagatctt
     8461 tggcatcggc ttcactcaac cagaccaccg gaccaccgcg acctaaagtc agcatcagca
     8521 ggttgtcgct gtaggtggag tggatacccc acttctggtg cggcgtcagg aagttgagcg
     8581 ctttttccgg gttgccgttg gatttctggc ctatcacttc tttcaccgaa cgggtgtcga
     8641 tcggcggacg ataaaccagc aggctttcac cgaaatcacg catccactgg tgatcctgat
     8701 acagttgctg acgaccagag agcgtacgcc atgggatcag ctcgtgaacg ttggtgtaac
     8761 cggcgttgta agaaacgtgt tcatcttcca gaccagacca ggtcgggctg gagataattt
     8821 tgcgcggctg tgcctgaata tcgaggaagc ggatcttctc gtcttcttta ttcagcgcca
     8881 gatgcgtatg gtcacgaccg gtaaattcgc tcagggcagc ccaggctttc acggctacct
     8941 gaccgttggt ttccggtgcc agtgtcagga tcatctctgc cgcatcaatt gcggtgttca
     9001 gcatcggctg gcctttcgcc ggaccttccg ctttggtgta gttgagctta cgcagcagat
     9061 ccatctcgct ctgggtgttc caggcaatcc ctttaccgcc attaccgatt ttctccatca
     9121 acgggccaat agaggtaaag cgttcgtaag tcgccggata atcgcgctct acgaccataa
     9181 tgtgtggcgc ggttttacct gggatcaggt cgcactcgcc ttttttccag tctttcacat
     9241 ccagcggctg cgccagttca gcggcagagt catgctggat aggcagcgtg acgatgtcgg
     9301 tttctttacc cagatggccg acgcacactt cggagaattt cttcgcgatg gctttgtaga
     9361 tttcccagtc gcttttcgct tcccaggccg gatcgaccgc cgcagacagc gggtgaataa
     9421 acggatgcat atccgaagta ttcatgtcgt ctttttcgta ccaagtcgcc gtcggcaaaa
     9481 tgatgtcgga atagagacag gtgctcgaca gacggaagtc cagcgtaacc accagatcca
     9541 gcttgccttc cagaccattg tcctgccagt ccacttcttc cggcttcacg ccgccctgtt
     9601 gccccagatc tttaccctgg ataccgtgct ccgtccccag caggtacttg agcataaact
     9661 catgaccttt accggaagaa ccgagcaggt tagaacgcca gatgaacagg ttgcgcgggt
     9721 ggtttttacc gttttctggt tgttctgccg caaaacggat ggaaccctct ttcagggatt
     9781 tcaccgtata gtccaccgga ttcatcccgg ctttttccgc ttcgccagcg atagtcagcg
     9841 ggttagtgcc taactgcggt gcagacggca gccagcccat gcgttccgca cggacgttaa
     9901 agtcgatcaa gtgtccggta tagcgggatt tgtccgccat cggtgacagc aactcttccg
     9961 ccgtgacggt ttcataacgc cactggctgg agtggttata gaaataagaa gtgctgttca
    10021 tgtgacgcgc cggacgctgc cagtcaaggg caaacgccag cggctgccag ccggtttgcg
    10081 gacgcagttt ttcctgacct acatagtgcg cccagccgcc cccgctctga ccgacacagc
    10141 cgcagaaaat cagcatgttg atcagaccac gatagttcat atcgaggtga taccagtggt
    10201 tcagccccgc accgacgata atcatcgaac gaccgtgcgt tttatcagcg ttatcggcaa
    10261 attcacgggc gatgcgaata atctggctgc gagaaacgcc ggtaatctgc tcggcccagg
    10321 ccggggtata agctttcaca tcgtcatagc tggttgcaca gttaacgtcg ttcaggccac
    10381 gttccagacc gtagtttgcc agcgtcagat cataaacggt gctcaccagg gcggtgctgc
    10441 catcagccag ttgcaggcgt ttcaccggca gtttgtgcag cagcacgttt tccagttcca
    10501 ctttgttgaa gtgttccgtg ccgtcgccac caaagtacgg gaagcctacc tcagcgatct
    10561 catcctggct acccagcagg ctcagttgca gctcggtttc ttcgccagtt ttgccgtcgc
    10621 gctgctcaag attccatttg cccttctcgc cccagcggaa gccaatagaa ccgttcggcg
    10681 caaccatttc gccattggta ttaaaggcga cagttttcca ttccggattg ttttcctggc
    10741 ccagcgcatc aaccagatca gcagcgcgca gcatacgacc tgcagcgtag taaccgtcgc
    10801 gttcttccag catcaccagc atcggcatgt cggtgtagcg acgcacatag tcggtgaaat
    10861 actggcttgg gttgtcgagg tggaattcac gcagcattac gtggcccatc gccagcgcca
    10921 ttgccgcatc ggtgccctgt ttcggtgcca gccacagatc gcacagtttg gcgatttcag
    10981 cgtagtctgg tgtgacggca acagttttgg tgcctttgta acgcacttca gtaaagaagt
    11041 gagcatccgg ggtacgcgtc tgcggcacgt ttgaacccca ggcgatgatg tagctggagt
    11101 tataccagtc agcagattcc ggtacgtcag tttgctcgcc ccaggtttgc ggagacgcag
    11161 gaggcaggtc gcagtaccag tcgtagaagc ttaagcaagt accgccaatc agcgagagat
    11221 agcgtgcacc cgatgcgtaa gaaaccatcg acattgccgg aattggcgag aaaccagcaa
    11281 cacggtccgg gccgtagttt ttgatggtgt aaacgttaga tgcggcgatc agttcgttca
    11341 cctcctgcca ggaagaacga acaaatccac cgcgtccacg cgcctgctta aagcttttcg
    11401 ctttatcggc gtcttcaatg atagaagccc atgcctcaac cggatcgcta tgcagcgcct
    11461 tcgcttcacg ccacattttc atcaggcgtt tgcgcatcat cgggtatttc aggcggttgg
    11521 cactgtaaag ataccaggag tagctggcac cgcgcgggca gccgcgaggt tcatggtttg
    11581 gcagatccgg acgggtacgc ggatagtcag tctgctgggt ttcccaggtg accagaccgt
    11641 ttttgacgta gattttccag ctgcaggagc cggtgcagtt taccccgtgg gtagagcgga
    11701 cgattttgtc atgctgccaa cgctggcgat atccatcctc ccagtcacgg ttggtattga
    11761 gaagctggcc atgcccatcg gcaaaggttt cacccttctg tttgaagtag cgaaaccggt
    11821 ccaggaattt actcatcggt tttctcctgt gggagcctgt cggccctctg ataaatcgac
    11881 attgctaatg tttaagggca aggtaacgct ctgaaacagc gtgggaattg ataacgatca
    11941 agagtgatgg ggatgaaaaa taaagtaaat tcctaagata cccctttagg ataattaaca
    12001 gccaccacat aacagactgt aaattaagac gttttatacc tgtagttatt taaactatta
    12061 agatttttat gcagatgggt att
//
LOCUS       DAZOZD010000120        12037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOZD010000120 DAZOZD010000000
VERSION     DAZOZD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.120"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHUPB_002690"
     CDS             <1..323
                     /locus_tag="ACHUPB_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759463.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHUPB_002691"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHUPB_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHUPB_002692"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHUPB_002692"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0759464.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="ACHUPB_002693"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="ACHUPB_002693"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0759465.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHUPB_002694"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHUPB_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0759466.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHUPB_002695"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHUPB_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0759467.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHUPB_002696"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHUPB_002696"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0759468.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="ACHUPB_002697"
     CDS             8424..8927
                     /locus_tag="ACHUPB_002697"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0759469.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="ACHUPB_002698"
     CDS             9004..9687
                     /locus_tag="ACHUPB_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0759470.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="ACHUPB_002699"
     CDS             9766..10752
                     /locus_tag="ACHUPB_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0759471.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="ACHUPB_002700"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="ACHUPB_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0759472.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAZOZD010000121        12034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZOZD010000121 DAZOZD010000000
VERSION     DAZOZD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.121"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            226..996
                     /locus_tag="ACHUPB_002701"
     CDS             226..996
                     /locus_tag="ACHUPB_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0759473.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            987..1460
                     /locus_tag="ACHUPB_002702"
     CDS             987..1460
                     /locus_tag="ACHUPB_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0759474.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1568..2107
                     /gene="dnaT"
                     /locus_tag="ACHUPB_002703"
     CDS             1568..2107
                     /gene="dnaT"
                     /locus_tag="ACHUPB_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0759475.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2110..2847
                     /gene="dnaC"
                     /locus_tag="ACHUPB_002704"
     CDS             2110..2847
                     /gene="dnaC"
                     /locus_tag="ACHUPB_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0759476.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2896..3390
                     /gene="yjjA"
                     /locus_tag="ACHUPB_002705"
     CDS             2896..3390
                     /gene="yjjA"
                     /locus_tag="ACHUPB_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0759477.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3644..5935
                     /gene="opgB"
                     /locus_tag="ACHUPB_002706"
     CDS             3644..5935
                     /gene="opgB"
                     /locus_tag="ACHUPB_002706"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0759478.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6074..7096)
                     /gene="lgoD"
                     /locus_tag="ACHUPB_002707"
     CDS             complement(6074..7096)
                     /gene="lgoD"
                     /locus_tag="ACHUPB_002707"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR0759479.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7235..8149
                     /locus_tag="ACHUPB_002708"
     CDS             7235..8149
                     /locus_tag="ACHUPB_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0759480.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8364..9715
                     /locus_tag="ACHUPB_002709"
                     /pseudo
     CDS             8364..9715
                     /locus_tag="ACHUPB_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9764..11419)
                     /gene="tsr"
                     /locus_tag="ACHUPB_002710"
                     /pseudo
     CDS             complement(9764..11419)
                     /gene="tsr"
                     /locus_tag="ACHUPB_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11538..11984)
                     /gene="hpaR"
                     /locus_tag="ACHUPB_002711"
     CDS             complement(11538..11984)
                     /gene="hpaR"
                     /locus_tag="ACHUPB_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0759481.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2924 a         3183 c         2942 g         2985 t
ORIGIN      
        1 gattcgtaaa ggccaaacaa taaattcatt atagtgataa tattcatgtg atagtttgtt
       61 tgcggcgaga gataattcgc tttttatcac cgagcttgtc ccgataacca ggattaatca
      121 gcgcaatttt gatcttaacc taccgcaaaa caaccgtgct tcatgataca aatggattct
      181 cattatctga acaatacgca gcacgtctat gacaaagggc gagttatgca aactgagcaa
      241 cagcgagccg taacacggct ttgtatccag tgtggattat ttcttttgca acatggtgcg
      301 gaaagcgcgt tggttgatga gctttcctca cgactgggtc gggcgttggg gatggacagc
      361 gtcgaaagtt ctatctcttc gaacgccata gtgctgacaa ccattaaaga tgggcaatgc
      421 ctgacatcga cacgtaaaaa tcacgatcgc ggcattaata tgcatgtggt gactgaagtc
      481 cagcacattg tgattctggc ggagcaccat ctgctggatt acaaaggcgt agagaaacga
      541 tttagccaaa ttcagccatt acgttaccca agatggctgg tagccttaat ggttggcctt
      601 tcttgcgcct gtttctgcaa actcaataaa ggtggctggg atggtgccgt catcaccttc
      661 tttgccagta cggccgcgat gtatatccgc cagctgctgg cacaacgtca tcttcatccg
      721 cagatcaact tttgcctcac cgctttcgcc gccaccacca tttccggatt gcttttgcaa
      781 cttcccactt tcagcaatac acccactatc gcgatggccg ccagcgttct gctgctggtg
      841 ccgggatttc cgttgattaa tgccgtcgcc gatatgttta aaggccacat caataccgga
      901 ctggcacgct gggcgatcgc cagtctgctg acactggcta cctgcgtcgg cgtggtgatg
      961 gcactgacga tttgggggct acgcggatgg gtgtgatcga gtttctgttt gcattggcgc
     1021 aggatatgat cctcgccgcc attccagcgg tcggctttgc gatggtgttc aacgttcccg
     1081 ttcgggcatt acgctggtgt gcgctgcttg gcgcgattgg tcatggttca cgaatgatct
     1141 taatgaccag cgggctgaat attgagtggt caacctttat ggcctccatg ctggtcggca
     1201 ccattggtat tcaatggtcg cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg
     1261 ccgttatccc tatgttcccg ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa
     1321 tcagccagtt aggttacagc gaaccgttga tgattaccct gttaaccaac tttcttacag
     1381 cttcatcgat tgttggtgcg ttatccatcg gtctttccat tcctggatta tggttgtacc
     1441 gcaagcgccc tcgcgtataa aattgcctcg cttaaagcac acggatgaga gacagcctcc
     1501 tctcctccgt gtgttactat aaaagttatc tcccttctcg ttcatcgttc catatttgag
     1561 aaacagtatg tcttccagag ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca
     1621 cgatcaccaa accgttctgg caaaagctga aggcggtgtg gttgccgtat ttgctaacaa
     1681 tgccccggcg ttttatgccg tcacgcctgc acgcctggct gaactgctgg cgctggaaga
     1741 aaagctggcg cgtccgggaa gcgatgtcgc tctggacgat caactctatc aggaaccgca
     1801 agccgctccc gttgctgtac ccatggggaa attcgccatg tatccggact ggcaacccga
     1861 tgccgatttt atccgcctgg cggcgctatg gggtgtggca ctaagagagc cggtgaccac
     1921 cgaagaactg gcctcattca ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt
     1981 gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt cgtgccagca acggcggact
     2041 gccgaaacga gatgtgaata cggtcagcga acctgacagt caaattccac caggattcag
     2101 agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc
     2161 catatcaaac ctgcattcaa aacgggtgaa gaactactgg cctggcagaa agaacaagga
     2221 gcgatccgct ccgccgctct cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt
     2281 aaccgctccg gtattcggcc actgcatcag aactgctcct ttgagaacta tcgcgttgag
     2341 tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt acgtcgaaga gtttgacggc
     2401 aacatcgcca gcttcatctt ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg
     2461 gcaatctgca acgagctgct gctacgcggt aaatccgtat tgatcatcac cgtggccgat
     2521 attatgtcgg cgatgaaaga taccttcagg aatagcggta ccagtgaaga acaactgctt
     2581 aacgatctga gcaacgttga tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg
     2641 aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc gctcttcttc caaacgccca
     2701 accgggatgc tgaccaacag taatatggaa gagatgacca agttgctggg cgaacgcgtg
     2761 atggaccgta tgcgcctggg taacagtttg tgggtgatct tcaactggga tagctaccgt
     2821 agccgggtaa caggtaaaga gtattaatcc ccgtttctgg cggtatactg cgacacaatt
     2881 ccaccttgag taatgatgat gaaaactgtt aaacatcttc tgtgctgtgc cattgccgcc
     2941 agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc
     3001 gccagcgaac ttggcaacca aaacagcacg acacaggaag gcggttggtc gctcgcgtca
     3061 ttaactaact tgcttagcag cgggaaccag gccttaagcg cagataacat gaacaacgcc
     3121 gcaggcattc tgcaatactg cgcgaagcaa aagctggcgt cggtaaccga tgccgaaaac
     3181 atcaagaacc aggtgctgga aaagctgggc ctgaacagtg aagagcaaaa agaagacacc
     3241 aactatctgg atggcattca gggtttgctg aaaaccaaag atggtcagca actcaatctg
     3301 gataacatcg gaacgactcc gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg
     3361 ttaaagcagg ggctgaactt catttcctga accgcgaaac gccgcgtttt agctgttacc
     3421 cggctgacgc ggctctctcc tgcccttttc cctgctcctg gacggttttc cccctgttac
     3481 acgggaagcg accgcgatca aaaaagtctg tttataaact aattcctaac aacatcgcat
     3541 ttttttgaca ctagaattgc agcaatatgg cgacgccatt acattgtgct tctcaaaaag
     3601 cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc ccgttgtcag aactactctc
     3661 tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg
     3721 gtggtttgca gccacattaa cggtgctggg gctatttgtc gttttaaata tcaccctgtt
     3781 tgccagcgac tattttactg gcgatggtat taacgacgcg gttctctata ccttaaccaa
     3841 cagcctgacc ggtgctggcg tcagcaaata catcctgccg ggtatcggca ttgtgctggg
     3901 gctgacagcg gtgttcggtg cgctgggctg gatcctgcgc cgtcgtcgcc atcatccgca
     3961 ccattttggt tacagcctgc tggcgctctt actggcgctg ggttcggtgg acgccagccc
     4021 ggcatttcgt cagataacgg aactggtgaa atcccagtca cgcgatggcg acccggactt
     4081 tgcggcttat tataaagagc cgtcgaaaac tatccctgac ccgaaactca acctggttta
     4141 tatctacggc gaaagtctcg agcggaccta ttttgataac gaagctttcc cggatctcac
     4201 gcctgaactg ggcgcgttga aaaatgaagg cctggatttc agccacacgc agcagctgcc
     4261 aggaacggat tacacgattg cgggcatggt ggcttctcag tgcggcatac cgctgtttgc
     4321 cccctttgaa ggcaacgcct ccgcctctgt ctccagcttc ttcccgcaga acatctgtct
     4381 gggcgatatc ctgaaaaact cgggttatca gaactatttc gtgcagggcg cgaatctgcg
     4441 ttttgccggt aaagatgtgt tcctgaagtc gcatggcttc gaccacttat acggctcaga
     4501 agagctgaaa agcgtggtgg ccgacccgca ctatcgcaac gactggggat tctacgacga
     4561 taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt tcccgctcag gtcagcgatt
     4621 ctcactgttt accctgacag tcgataccca tcacccggat ggttttatct cgcgtacctg
     4681 taaccgcaaa aaatatgatt ttgacggtaa gccgaaccag tcattcagcg cggtaagttg
     4741 cagccaggag aatatcgcgg cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga
     4801 taccgtcatc gtcgtctctt ctgaccattt agcgatgaac aacacggcgt ggaaatacct
     4861 caataagcag gatcgcaata acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga
     4921 gacgctggca gtgaagcgta acacgatgga taacggcgcg acggtgctgg acattctcgg
     4981 tggcgataac tatctcggac ttggtcgtag cagtttatcc gggcagtcga tgtcggaaat
     5041 cttcctcaat atcaaagaga aaacattggc gtggaagccg gatatcatcc gcctgtggaa
     5101 attccctaaa gagatgaaag agttcaccat cgaccagcag aaaaacatga ttgccttctc
     5161 gggtagccat ttccgtttgc cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct
     5221 gccggaaagc gaatactcag caccgctgcg tttccagctg gccgatttcg ctccacgcga
     5281 caatttcgtc tgggttgacc gttgctataa gatggcacaa ctctgggctc cggaactggc
     5341 actctccacc gactggtgtg tctcgcaagg gcagcttggc ggtcagcaaa ttgttcagca
     5401 tgttgacaaa acaacatggc agggcaaaac ggcatttaaa gatacggtca tcgacatggc
     5461 gcgttataaa ggcaatgtcg atacgctgaa aattgttgat aacgatattc gctacaaagc
     5521 cgacagtttc atcttcaacg tcgccggtgc gccagaagag gtgaaacagt ttagtgggat
     5581 ttcccgtcca gagtcgtggg gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat
     5641 cgagtacaag catccgctgc caaagaaatt tgacctggtg attaccgcca aagcatacgg
     5701 caataacgcc agccgtccta ttccggtacg cgtaggcaat gaagaacaaa cccttgtgct
     5761 gggcaatgaa gtgaccacca ccacactgca tttcgataac ccaaccgatg ccgacacact
     5821 ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg aatatcctcg gacactcgcc
     5881 gcgtaagctc gggatcggca tggtggaaat taaagtggtg gaacgtgaag gataattcct
     5941 gactgatgtg taggtcgcat aaggtgcgac ctacacacct tctgcggcct acaaaacatc
     6001 attgagaaac agaaaaaaac agcgcccaac acttccccgg acgctgcatg gcaactttaa
     6061 taatctcgcc ttatcagaaa gtaattacac ccttaatcaa ctcacgattg ttaatcacat
     6121 cgcgctcgta ggtttctgcc agcgtggcga acggatagcg atgggttaac atcatgtcag
     6181 cagtgatttt tccttccgcc atcagtcgac cgactttagc aaaatcttcc ggcgtggcgt
     6241 tgcggctacc catcatcgtc gtttcttttt tatggaattc cggatcggag aactgcaact
     6301 cacctttaaa caggccgaca aataccaccg tgccgccgtg acgaatcaaa ttcacggtgt
     6361 tattcatcgc atgttgatta cctgtcgcgt cgatcacttt ctgcgccagc gaaccaccaa
     6421 actgcgcccg tagctgcgcg tcaaaatctt cggctgacgg gtccagtaaa ggtaattcca
     6481 gacgcgttgc cacatgttca cggcgcgccg gactggtatc cgccaccacc acctgtgcgc
     6541 catcggcttt agcgattgcc gccgcgccca gaccgattgg ccccgccccg accaccagca
     6601 cctgctcgcc gggagcaatg gctgcgcgac gcaccgcatg agcgctaata gcgaaaggtt
     6661 caatcaatgc tgccgcctgc gggtcaatac cgtctgcggg caaaatgttc gccaccggca
     6721 cgctcaaata ctcactaaaa ccgccatcct gatgcacgcc aatgactgaa attttttcac
     6781 agcaattggt acgcccgctt ttacacgccg ggcattgctg acaggcaaca taagggatca
     6841 cagcaacttg ctgaccattt ttaagatcag caatattttt acccagccca acaatctccc
     6901 cacatatttc atggcctaaa acacgtggat aactaaaaaa tggttgattt ccaccccagg
     6961 catgaatatc ggtaccgcaa atcccgacag acttaatttt tattaatgct tcattgtcac
     7021 ccggaatagg tatctcgcgt tgtttccaga ctaattcttt cggctgctgg caaattaaaa
     7081 cattcatcgt agacatgatt cggctctcca ttttttgttg ccttagatat caatgaaaat
     7141 gaaaaatggc agcggaacgg aaaatctgtt ttgtgaaaac acacgcataa aaatgtttta
     7201 aatcgggttt taatggactc acaaggagga aaatatgagt cgttcacaaa atttacgcca
     7261 caatgtgatt aaccaggtca ttgatgatat ggcgcggggt catatccctt ccccacttcc
     7321 atcgcaaagt gcgctggcgg aaatgtacaa catcagccgc accacagtgc gtcatattct
     7381 cagccactta cgcgaatgcg gcgtcttgac gcaggttgga aacgactatg ttattgcgcg
     7441 taaacctgac catgatgacg gtttcgcctg tactaccgct tcaatgagtg aacaaaacaa
     7501 agtgtttgag caggcttttt tcacgatgat aaaccagcgc cagttacgcc caggggaaac
     7561 gttttctgaa ctgcaactgg ctcgggccgc gggtgtcagc ccggttgtag taagagaata
     7621 tcttttaaaa ttcggccgtt ataatcttat tcacagcgaa aagcgcggcc agtggagcat
     7681 gaagcaattc gatcaatcct acgccgagca gctgtttgag ctacgcgaaa tgctcgaaac
     7741 acattcactt cagcactttc tcaacctgcc tgatcatgat ccccgctggc tgcaggcgaa
     7801 aaccatgctc gaacgtcatc ggctactgcg tgacaacatc ggcaatagct ttcgcatgtt
     7861 ctcgcaactt gacagagatt ttcactcctt actgctttca gctgccgaca atatcttttt
     7921 tgatcaatcg cttgagatca tctcagtcat ctttcatttt cactatcaat gggacgaaag
     7981 cgatcttaaa cagcgcaata tcattgccgt cgatgagcat atgaccattc tcagcgctct
     8041 gatttgccgc agtgaccttg atgccaccct tgcgctccgt aaccatttga attcagctaa
     8101 acaatcaatg attcgatcca ttaatgagaa cacgcgttat gctcattaag tacggattaa
     8161 aaacagcaaa ctattagcgg gtgcacggtt ttatcaaaag ataaataccg ggaaattccc
     8221 gcttacctat gctcacaatc agtcgactaa aaatgttttt gtgagcggta gtaaagtcct
     8281 aaaactttaa cctgccgtta atgtcaacct tgcgtaattc acaattacgt aaacaattaa
     8341 aattataaat acaggagtcc ggcgtggaaa aagaaaatat caccctcgat ccgcgttctt
     8401 catttactcc atcttcgtcg gcagatattc ccgtgccacc agatggatta gttcaacgca
     8461 gtacccgaat taaacgcatt caaaccaccg ccatgttgtt attatttttt gcggcggtaa
     8521 tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt aacgattcgt gaagaattgg
     8581 gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt ttcactcgct tacgggattg
     8641 cgcaacttcc ttgcggccca ctattggatc gtaaaggccc acgcctgatg ctgggactgg
     8701 ggatgttctt ctggtcactg ttccaggcaa tgtctggcat ggtgcacaac tttacgcagt
     8761 tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc gatgaaccca tgcggtgtaa
     8821 aagtcattaa cgactggttc aacatcaaag agcgcggacg cccgatgggc ttcttcaacg
     8881 cagcttctac cattggcgtt gccgtaagcc caccgattct ggcggcgatg atgctggtga
     8941 tgggctggcg cgggatgttt attaccattg gtgtactggg gatttttctc gccatcggct
     9001 ggtatatgct ctatcgcaac cgcgagcacg tagaactgac tgccgttgaa caagcttatc
     9061 tcaatgcagg tagcgtcaat gcccgccgag atccgctcag ttttgccgaa tggcgcagcc
     9121 tgttccgtaa ccgtacaatg tggggaatga tgctcggatt cagtggcatc aactacactg
     9181 cgtggctgta tctggcctgg cttcctggtt acctgcaaac agcctataac ctggatttaa
     9241 aaagcacagg gttgatggcg gctatccctt tcctgtttgg ggctgccggg atgctggtca
     9301 acggttacgt tactgactgg ctggtcaaag ggggaatggc tccgattaaa agccgtaaga
     9361 tctgcattat tgccgggatg ttctgttctg ccgcctttac gctggtcgta ccgcaagcga
     9421 caacatccat gacagcggtt ctgctgattg gcatggcact gttctgtatt caacatcctg
     9481 ctggggcttg atccacgtcg cagttgcttc tcgcatgact gcgtcggtgg gcagtatcca
     9541 gaactttgcc agcttcatct gcgcctcttt tgcgccgatc attactggtt ttattgttga
     9601 taccacccac tcattccgtc tggcactaat catctgcggt tgcgtcaccg cagcgggggc
     9661 actggcgtac atcttcctgg ttcgtcagcc gatcaacgac ccacgtaaag attaatctcc
     9721 ttatgcccga taacattttg cttatcgggc attttcatgg cgattaaaat gtttcccagt
     9781 tctcctcgct atctgccacg gccattttac gcggcgcagc tggcgtcacg gtttttacca
     9841 cagccgatgt ttcacgctgc tgttgctgaa tccggaacac tgccactgct tcggtcaggc
     9901 gactggcctg ctcttccagc gcggcggcgg cagcggcaga ctcttccacc agcgcggcgt
     9961 tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg ataccacggc
    10021 tctgctcatc agaagcagaa gcaatttcgc ccataatgtc cgtcacgcgg gtcacggcgc
    10081 tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca acatccactt
    10141 tccccaccga gtcttcaatc aggcttttaa tttcacgagc cgcctgggcg ctgcgctggg
    10201 ccagattacg cacttctccc gcgaccaccg caaaaccgcg cccttgctca cccgcacgcg
    10261 cagcctcaac cgccgcgttc aaagccagaa tattggtctg gaaggcaatg ccgtcaatta
    10321 cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata gtctgcacca
    10381 cgttatctac cactttgccg ccgcgttgcg ccgtttcaga agcacttaac gccagatggc
    10441 tggcctggcg cgcattttcg gcgttctgct tcaccgttgc ggtcagttac tccatgctgg
    10501 ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga tcgttattgc
    10561 cggtggcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc acatcaccga
    10621 cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc cccatctcat
    10681 tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt
    10741 caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag acggcgaaga
    10801 tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg ctgtaggagg
    10861 cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat
    10921 actgcttctc gaaaccgtcc tgatatccct gggtcggctg atcaaagaac tcgttgattt
    10981 tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac
    11041 gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat
    11101 ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa
    11161 cggttgaacc gctaccaata ttgttctgat ccatcatgta gcggataccc gcgcggttga
    11221 gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtggat tgctgctggc
    11281 gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta aagaacagac
    11341 cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga
    11401 tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat tttcggcctg
    11461 gaaagcaaaa actttatgaa aaggtgtaac ccaccatcca tggaaaaacc aggcgctggt
    11521 tatcagatac taaaaagtta ttcattatcg cccgggatgg cttcttgtcg ggaattgccc
    11581 agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc agtaaactga
    11641 gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc
    11701 ccctctttgg ttagcgagat atagagcttc cgctgatcat tgatcggctt taatcgcaac
    11761 actaaaccgt cgcgttccat ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag
    11821 gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat acgcacgatg
    11881 cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata actcatcgcc
    11941 gcttctcgcg cctggagcaa cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa
    12001 aattcaaaat cattaatata gaaacagtta atcg
//
LOCUS       DAZOZD010000122        11990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOZD010000122 DAZOZD010000000
VERSION     DAZOZD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.122"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            147..1256
                     /gene="livK"
                     /locus_tag="ACHUPB_002712"
     CDS             147..1256
                     /gene="livK"
                     /locus_tag="ACHUPB_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0759482.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1304..2230
                     /gene="livH"
                     /locus_tag="ACHUPB_002713"
     CDS             1304..2230
                     /gene="livH"
                     /locus_tag="ACHUPB_002713"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0759483.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2227..3504
                     /gene="livM"
                     /locus_tag="ACHUPB_002714"
     CDS             2227..3504
                     /gene="livM"
                     /locus_tag="ACHUPB_002714"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0759484.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3501..4268
                     /gene="livG"
                     /locus_tag="ACHUPB_002715"
     CDS             3501..4268
                     /gene="livG"
                     /locus_tag="ACHUPB_002715"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0759485.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4270..4983
                     /gene="livF"
                     /locus_tag="ACHUPB_002716"
     CDS             4270..4983
                     /gene="livF"
                     /locus_tag="ACHUPB_002716"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0759486.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5267..6331
                     /gene="ugpB"
                     /locus_tag="ACHUPB_002717"
                     /pseudo
     CDS             5267..6331
                     /gene="ugpB"
                     /locus_tag="ACHUPB_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6429..7316
                     /gene="ugpA"
                     /locus_tag="ACHUPB_002718"
     CDS             6429..7316
                     /gene="ugpA"
                     /locus_tag="ACHUPB_002718"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0759487.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7313..8158
                     /gene="ugpE"
                     /locus_tag="ACHUPB_002719"
     CDS             7313..8158
                     /gene="ugpE"
                     /locus_tag="ACHUPB_002719"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0759488.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8160..9230
                     /gene="ugpC"
                     /locus_tag="ACHUPB_002720"
     CDS             8160..9230
                     /gene="ugpC"
                     /locus_tag="ACHUPB_002720"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR0759489.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9227..9970
                     /gene="ugpQ"
                     /locus_tag="ACHUPB_002721"
     CDS             9227..9970
                     /gene="ugpQ"
                     /locus_tag="ACHUPB_002721"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0759490.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(9957..10397)
                     /locus_tag="ACHUPB_002722"
     CDS             complement(9957..10397)
                     /locus_tag="ACHUPB_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0759491.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10517..>11990
                     /gene="ggt"
                     /locus_tag="ACHUPB_002723"
     CDS             10517..>11990
                     /gene="ggt"
                     /locus_tag="ACHUPB_002723"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0759492.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         2603 a         3085 c         3464 g         2838 t
ORIGIN      
        1 agggatttta atcactgtta tcacaaaata ctgcgctaaa cccttaatca gacaggcaaa
       61 aacagtgcag tataaaaaaa gaacagtctg atttgttaac acataaaaac aaagcaacac
      121 aacatcacga atggggattt ttgactatga aacggaatgc gaaaactatc atcgcaggga
      181 tgattgcact ggcaatttca cacaccgcta tggctgacga tattaaagtc gccgttgtcg
      241 gcgcgatgtc cggcccgatt gcccagtggg gcgatatgga atttaacggc gcgcgtcagg
      301 cgattaaaga cattaatgcc aaagggggaa ttaagggcga caagctggtt ggcgtggaat
      361 atgacgacgc ctgcgacccg aaacaagccg ttgcggtcgc caacaaaatc gttaatgacg
      421 gcattaaata cgttattggt catctgtgtt cttcttctac ccaacctgca tcagatatct
      481 acgaagacga aggtattctg atgatctcgc cgggagcgac caacccggag ctgacccaac
      541 gcggttatca acacattatg cgtactgccg ggctggactc ttcccagggg ccaacggcgg
      601 caaaatacat tcttgagacg gtgaagcccc agcgcatcgc catcattcac gacaaacaac
      661 agtatggcga agggctggcg cgttcggtgc aggacgggct gaaagcggct aacgccaacg
      721 tcgtcttctt cgacggtatt accgccgggg agaaagattt ctccgcgctg atcgcccgcc
      781 tgaaaaaaga aaacatcgac ttcgtttact acggcggtta ctacccggaa atggggcaga
      841 tgctgcgcca ggcccgttcc gttggcctga aaactcagtt tatggggccg gaaggtgtgg
      901 gtaacgcatc attgtcgaat attgctggcg atgctgccga aggcatgttg gtcactatgc
      961 caaaacgcta tgaccaggat ccggcaaacc agggcatcgt tgatgcgctg aaagcagaca
     1021 agaaagatcc gtccgggcct tatgtctgga tcacctacgc ggcggtgcaa tctctggcga
     1081 ctgcccttga gcgtactggc agcgatgagc cgctggcgct ggtgaaagat ttaaaagcta
     1141 acggtgcaaa caccgtgatt gggccgctga actgggatga aaaaggcgat cttaagggat
     1201 ttgattttgg tgtcttccag tggcacgccg atggttcatc cacggcagcc aagtgatcat
     1261 cccaccgccc gtgaaatgcg ggcgggttta gaaaggttac cttatgtctg agcagttttt
     1321 atatttcttg cagcagatgt ttaacggcgt cacgctgggc agtacctacg cgctgatagc
     1381 catcggctac accatggttt acggcattat cggcatgatc aacttcgccc acggcgaggt
     1441 ttacatgatt ggtagctatg tctcctttat gatcatcgcc gcactgatga tgatgggcat
     1501 tgataccggc tggctgctgg tggcggcggg attcgtcggc gcaatcgtca ttgccagcgc
     1561 ctacggctgg agtatcgaac gggtagccta tcgcccggtg cgtaactcta agcgcctgat
     1621 tgcgctcatc tcggcaatcg gcatgtccat cttcctgcaa aactacgtta gcctgaccga
     1681 aggttcgcgt gacgtggcgc tgcccagtct gtttaacggt cagtgggtgg tggggcatag
     1741 cgaaaacttc tctgcctcta ttaccaccat gcaggcagtg atctggattg ttactttcct
     1801 cgccatgctg gcgctgacga ttttcattcg ttattcccgt atggggcgcg cctgccgtgc
     1861 ctgcgcggaa gatctgaaaa tggcgagcct gcttggcatc aacaccgacc gggtgattgc
     1921 tttgaccttt gttattggcg cggcgatggc ggcagtggct ggcgtactgc tcggtcagtt
     1981 ctacggcgtc attaacccct atatcggctt tatggccggg atgaaagcct ttaccgctgc
     2041 ggtgctcggt gggattggca gcattccagg ggcgatgatt ggcgggctga tcctggggat
     2101 tgcggaggcg ctctcttctg cctatctgag tacggaatat aaagatgtgg tctcattcgc
     2161 cctgctgatt ctggtgctgc tggtgatgcc gaccggtatt ctgggtcgcc cggaggtaga
     2221 gaaagtatga aaccgatgca tattgcaatg gcactgctct ctgccgcgat gttctttgtc
     2281 ctcgctggcg tctttatggg cgtgcaactg gagctggatg gcaccaaact ggtggtcgac
     2341 agcgcttcgg atgtccgttg gcagtgggtg tttatcggca cggcggtggt ctttttcttc
     2401 cagcttttgc gacctgcttt ccagaaaggg ctaaaaagcg tttccgggcc gaagtttatt
     2461 ctgcccgcga ttgatggctc cacggtgaag cagaaactgt tcctcgtggc gctgctggtg
     2521 cttgcggtgt cgtggccgtt tatggtgtca cgtgggacgg tggatatcgc cactctgacc
     2581 atgatctaca ttatcctcgg tctcgggctg aatgtggttg ttggtctttc tggtctgctg
     2641 gtgctggggt acggcggttt ttacgccatc ggcgcttaca cttttgcgct gctcaatcac
     2701 tattacggtc tggggttctg gacctgtttg ccgattgccg gactgatggc agcggcggcg
     2761 ggcttcctgc ttggttttcc ggtgttgcgt ctgcgcggtg attatctggc gatcgttacc
     2821 ctcggtttcg gcgaaattgt gcgcatattg ctgctcaata acaccgaaat taccggcggc
     2881 ccgaacggta tcagtcagat cccgaaaccg actttcttcg gccttgagtt cagccgtacc
     2941 gcccgtgaag gcggttggga cacgttcagt aatttctttg gcctgaaata cgatccctcc
     3001 gatcgcgtca tcttcctgta tctggtggcg ttgctgctgg tggtgctaag cctgtttgtc
     3061 attaaccgcc tgctgcggat gccgctgggg cgtgcgtggg aagcgttgcg tgaagatgag
     3121 atcgcctgcc gttcgctggg cttaagcccg cgtcgtatca agctgactgc ctttaccatc
     3181 agtgccgcgt ttgccggttt tgccggaacg ctgtttgctg cgcgccaggg ctttgtcagc
     3241 ccggaatcct tcacctttgc tgaatcggcg tttgtactgg cgatagtggt gctcggcggt
     3301 atgggatcgc agtttgcggt gattctggcg gcaattttgc tggtggtatc gcgcgaattg
     3361 atgcgtgatt tcaacgaata cagcatgtta atgctcggtg gtttgatggt gctgatgatg
     3421 atctggcgtc cgcagggctt gctgcccatg acgcgcccgc aactgaagct gaaaaacggc
     3481 gcagcgaaag gagagcaggc atgagtcagc cattattatc tgtgaacggc ctgatgatgc
     3541 gcttcggcgg cctgctggcg gtgaacaacg tcaatcttga actgtacccg caggagatcg
     3601 tctcgttaat cggccctaac ggtgccggaa aaaccacggt ttttaactgt ctgaccggat
     3661 tctacaaacc caccggcggc accattttac tgcgcaatca gcacctggaa ggtttaccgg
     3721 ggcagcaaat tgcccgcatg ggcgtggtgc gcaccttcca gcatgtgcgt ctgttccgtg
     3781 aaatgacggt aattgaaaac ctgctggtgg cgcagcatca acaactgaaa accgggctgt
     3841 tctctggcct gttgaaaacg ccttctttcc gtcgcgccca gagcgaagcg ctcgaccgcg
     3901 ccgccacctg gcttgagcgc attggtttgc tggaacacgc caaccgtcag gcgagtaacc
     3961 tggcctatgg tgaccaacgc cgtctggaga ttgcccgctg catggtgacg cagccggaga
     4021 ttttaatgct cgacgaaccg gcggcaggtc ttaacccaaa agaaaccaaa gagctggatg
     4081 agctgattgc cgaactgcgc aatcatcaca acaccactat cttgttgatt gaacacgata
     4141 tgaagctggt gatggggatt tcagaccgaa tttatgtggt caatcagggg acgccactgg
     4201 caaacggtac gccggagcaa atccgtaata acccggacgt gatccgtgcc tatttaggtg
     4261 aggcataaga tggaaaaagt catgttgtcc tttgacaaag tcagcgccca ctacggcaaa
     4321 atccaggcgc tgcatgaggt gagcctgcat atcaatcagg gcgagattgt caccctcatt
     4381 ggcgcgaacg gggcggggaa aaccaccttg ctcggcacgt tatgcggcga tccgcgcgct
     4441 accagcgggc gaattgtgtt tgatgataaa gacattaccg actggcagac ggcgaaaatc
     4501 atgcgcgaag cggtagcgat tgtcccggaa gggcgtcgcg tcttctcgcg gatgacggtg
     4561 gaagagaacc tggcgatggg cggctttttt gccgaacgcg accagttcca ggagcgcata
     4621 aagtgggtgt atgaactgtt cccacgtctg catgagcgcc gtgttcagcg ggcaggcacc
     4681 atgtccggcg gtgagcaaca aatgctggcg attggtcgcg cgctgatgag caacccgcgt
     4741 ttgctgctac ttgatgagcc atcgcttggt cttgcgccga ttatcatcca gcaaattttc
     4801 gacaccatcg agcagctgcg cgagcagggg atgactatct tcctcgtcga gcagaacgcc
     4861 aaccaggcgc taaagctggc ggatcgcggc tacgtgctgg aaaacggcca tgtggtgctc
     4921 tccgatactg gtgatgcact actggcgaat gaagcggtga gaagtgcgta tttaggcggg
     4981 taataacacg ttgattgata aggagtcaaa agactccttt gagacaggtg acaaatgtaa
     5041 aattgcctga tgcgctgcgc ttatcaggcc tactgggtga gttgcaatat attgaatttg
     5101 cacgatcttg taggccggat aaggcgttta cgccgcatcc ggcatgaaac gatgagtaat
     5161 ctgtagagtt tgattcagac cttctatttt tccgcttatc cgtgccccat ctcccatttt
     5221 ccctcaccca cgccgtcacc gccttgtcat ctttctgaca ccttacttgc aggtttatga
     5281 agttggcacc gccaccatga tggcatcgaa agccattaaa ccggtatatg acgtgtttaa
     5341 agaggcaggg attcaattcg atgagtcgca gtttgtgccg acggtttccg gttactactc
     5401 cgacagcaaa acgggccact tactctccca gccattcaac agctcgactc ccgttctcta
     5461 ttacaacaaa gacgccttca agaaagcggg gttagacccg gaacagccgc cgaaaacctg
     5521 gcaggatctg gcggactctt ccgcaaaact gaaagcctcc ggtatgaagt gcggctacgc
     5581 cagcggctgg cagggctgga tccaactgga aaactttagc gcctggaacg gtctgccgtt
     5641 tgccagcaaa aacaacggct ttgacggcac ggacgcggtg ctggagttca acaagccgga
     5701 gcaggtgaaa cacattgcca tgctcgaaga gatgaacaag aagggcgact tcagctacgt
     5761 cggtcgtaag gatgaatcca ccgagaagtt ctataacggt gattgcgcga tgaccaccgc
     5821 ctcttccggt tctctcgcta atattcgtga gtacgccaaa tttaactatg gcgtaggcat
     5881 gatgccttac gacgccgatg cgaaagatgc accgcaaaac gccattatcg gcggggccag
     5941 cctgtgggtg atgcagggca aaaataaaga aacgtatacc ggcgtggcga agttcctcga
     6001 cttcctcgcg aagccagaaa acgctgccga gtggcatcag aaaaccggtt atctgccaat
     6061 caccaaagca gcgtatgacc tgacccgtga gcagggcttt tatgagaaaa acccaggggc
     6121 ggataccgcg acgcgtcaga tgctgaataa gccgccgttg ccgttcacca aagggctgcg
     6181 tctgggcaac atgccgcaga tccgcgtgat tgtggatgaa gagctggaga gcgtgtggac
     6241 cggtaagaag acaccacagc aggcactgga taccgccgtt gagcgtggaa atcagttgct
     6301 gcgccgcttt gagaaatcga cgaagtctta atcagtgtaa tgtcggatgc gtttcgctta
     6361 tctgacctgg catcgcgtgt aggccggata agcgaagcgc atccggcaca gttcaggaat
     6421 taaccgtaat gtcatcatcc cgtccggtgt tccgctcgcg ctggctgcct tatctgctgg
     6481 tcgcgccgca gctcatcatc accgttatct tttttatctg gcctgcgggc gaagcgttgt
     6541 ggtactcgcg gcaaagcgtc gatccgtttg gtttctccag ccagtttgtc ggtctggata
     6601 acttcgtcac gctgttccat gacagctact atctagactc cttctggacg acgataaaat
     6661 tcagcacctt tgtcaccgtc agcggtttgc tggtatcgct gttctttgcg gcgctggtgg
     6721 agtacatcgt gcgcggcagc cgtttctatc aaaccttgat gctgctgccg tatgccgtgg
     6781 ctcccgccgt tgccgccgta ttgtggatct tcctgtttaa ccccggtcgc gggctgatca
     6841 cccattttct cgctgagttc ggctacgact ggaaccacgc gcaaaacagc ggtcaggcaa
     6901 tgtttctggt ggtgtttgcc tcggtgtgga agcaaatcag ctacaacttt ctgttcttct
     6961 atgccgcgct gcactccatt ccccgttcgt tgattgaagc cgcagctatt gacggcgcag
     7021 ggccgattcg ccgcttcttt aagattgctc tgccgcttat cgctccggtg agtttcttcc
     7081 tgctggtggt gaacctggtg tacgccttct tcgacacctt cccggtgatc gacgccgcca
     7141 cgtccggcgg gccggttcag gccaccacta cgctgattta taagatctac cgcgaaggct
     7201 ttaccggact ggatctggct tcgtctgccg cgcagtcggt ggtgttgatg ttcctcgtca
     7261 tcgtgctgac ggtggtgcag ttccgctatg ttgaaagcaa ggtgcgttac caatgattga
     7321 gaaccgtccg tggctgacga tattcagcca taccatgctg atcctcggga tcgcggtgat
     7381 cctcttcccg ctgtacgtgg cgtttgtcgc ggcgacgctg gataaacagg ccgtctatgc
     7441 cgcgccgatg acgctcatcc ccggcacaca tctgctggaa aatatccaca acatctgggt
     7501 taacggggta ggcacgaata gcgcgccgtt ctggcggatg ttgcttaaca gctttgtgat
     7561 ggcgttcagc attacgctcg gcaaaattac cgtctcgatg ctctcggcat ttgccattgt
     7621 ctggtttcgt tttccgctac gtaacctctt cttctggatg atttttatca ccctgatgct
     7681 gccggttgaa gtacgtatct tcccgacggt ggaagtcatc gccaacctga aaatgctcga
     7741 cagctacgcc ggtttaacgc tgccgctgat ggcctcggcg accgccacgt ttctgttccg
     7801 ccagttcttt atgaccctac cggatgaatt ggtggaagcc gcgcggatcg acggcgcgtc
     7861 accaatgcgt ttcttttgcg acatcgtttt tccgttgtcc aaaactaatc tggcggcgct
     7921 gtttgtgatc acctttatct acggctggaa tcagtatttg tggccgttgt tgattattac
     7981 cgacgttgat ctcggcacca ccgtggcagg gatcaaaggg atgatcgcta caggcgaagg
     8041 caccacggaa tggaactcgg taatggcggc gatgttgtta acgcttatcc ctccggtggt
     8101 gattgtttta gtgatgcagc gtgccttcgt gcgcggcctg gtcgatagtg agaaataaga
     8161 tggcaggact gaaattacag gcagtaacca aaagctggga tggcaaaacc caggtcatta
     8221 aaccgctgac ccttgatgtg gcggatggcg aatttatcgt gatggtcggg ccgtctggct
     8281 gcgggaaatc gacgctgctg cgcatggttg ccgggctgga gcgggtgaca gaaggcgata
     8341 tctggatcaa cgaccagcgc gtgaccgaaa tggagccgaa agatcgcggg attgcgatgg
     8401 tgttccagaa ttacgcgctt tatccgcata tgagcgtaga ggagaacatg gcgtgggggc
     8461 tgaaaattcg cggcatgggc aagcagcaaa ttgccgagcg cgttaaagaa gcggcgcgca
     8521 ttctggaact ggacggtctg cttaagcgtc gtccgcgtga gctatccggc ggtcagcgcc
     8581 agcgtgtggc gatgggccgc gcgattgtgc gcgatccggc ggtgttcttg tttgatgagc
     8641 cgctctctaa cctcgatgcc aagctgcgcg tgcagatgcg tcttgaactg caacagttgc
     8701 accgtcgcct gaaaacgact tcactctacg ttactcacga tcaggttgaa gcgatgacgc
     8761 tcgcccagcg agtaatggtg atgaacggtg gcgttgccga acagattggc acaccagttg
     8821 aagtctacga aaagcccgcc agcctgtttg tggcgagttt tatcggcagc ccggcgatga
     8881 acctgctgac aggccgcgtg aataatgaag gcacgcattt cgaactggac ggcggtatcg
     8941 tgctaccgct aaacggtggc taccgtcagt atgccgggcg taaaatgact ctcggcattc
     9001 gcccggaaca tatagcgcta agctcgcagg cagaaggtgg tgtgccgctg gtgatggaca
     9061 cgctggagat cctcggcgca gataacctgg cgcacggacg ctggggcgag cagaagctgg
     9121 tggtgcgact ggcgcatcag gagcgcccga cggcaggcag cacgctgtgg ctgcatttgg
     9181 cggaaaatca gctgcatctt tttgatggtg aaacaggaca acgagtatga gtaactggcc
     9241 ttatccccgc atcgtcgctc atcgtggcgg cggtaagctg gccccggaaa acaccctggc
     9301 ggcaatcgac gtcggggcaa aatacggtca taagatgatc gaatttgacg cgaagttatc
     9361 gaaagatggc gagatcttcc tgctccatga cgacaatctc gaacgcacca gcaacggctg
     9421 gggcgtcgcg ggtgaactga actggcagga tttactgcgc gtggatgcgg gcagttggta
     9481 cagcaaagcg tttaaaggtg agccgctgcc gttgctttcg caggtggcgg aacgctgtcg
     9541 cgaacacggg atgatggcga atatcgaaat caaacccacc actggcactg gaccattaac
     9601 gggcaaaatg gtggcgctgg cggcacgcca actgtgggcc ggtatgacgc cgccgctgct
     9661 gtcatcgttt gagattgatg ctttagaagc tgcacaacag gcggcaccgg aactgccgcg
     9721 tggtttattg cttgatgagt ggcgcgacga ctggcgcgaa ctgaccgcgc ggctgggctg
     9781 cgtctctatt catctcaatc ataagttact cgataaagcg cgagtgatgc agttgaaaga
     9841 cgccggatta cggattctgg tttataccgt caataaaccc cagcgcgcgg cagagttgct
     9901 gcgctggggc gtggattgca tctgtaccga tgcgattgac gtgattggtc cgaactttac
     9961 ggcccaatag ttttcaacgg aatgtcaggc tgcggcgtgc tgggctgatt cagcatgtcg
    10021 ccgtttctcc tttccggcag catatgctgc tgattcaacg aactatccgg attacggttg
    10081 ctgtttaaca tcccgccgtt ggtgttgggc agcatttgct gccgggcggg atttcgctcc
    10141 cccggctgtg actgcaacac ccgctgagaa ttattgttta tctggttttc taaatgctgc
    10201 tgttgcaact gagtttgcgt tttcagttgc tgattcagca tccctttttg ctggatttgc
    10261 tgagtctgca tctgggtttg catccgctgc tggctgggta tctgataccc cggctggtta
    10321 gggttgttca gagtattaat gggctgtgca aagccgacaa acggcaggag tgccgtaaga
    10381 atcagaagtc gtttcatcgc gtatcctcct ctgaagatat cctttaagtt tactcgcttc
    10441 ccgacaaaac gatgattaat tcagagttat ataccaggct tagctggggt tgccccttaa
    10501 tctctggaga ataacgatga taaaaccgac gtttttacgc cgggtggcca ttgctgctct
    10561 gctctcagga agttgtttta gcgccgccgc cgcgcctcct gcgccgcccg tctcgtatgg
    10621 tgtggaggaa gatgtcttcc acccggtacg cgcgaaacag ggaatggtag cgtctgtgga
    10681 cgccactgcc actcaggtgg gggtggatat cctcaaggag ggcgggaatg ccgttgatgc
    10741 cgccgtggcg gtgggctacg cgctggcggt aacgcatccg caggcaggga atctgggcgg
    10801 tggtggtttt atgttaatcc gctcgaaaaa tggcaatacc acggctatcg atttccgcga
    10861 aatggcaccc gccaaagcga cccgcgatat gttcctcgat gatcagggca acccggacag
    10921 caaaaaatca ctcacttcgc atctggcttc cggcacaccg ggtacggtag caggtttctc
    10981 gctggcgctg gataaatacg gcaccatgcc gctgaacaaa gtcgtgcagc ccgcgtttaa
    11041 actggcacgc gatggtttta tcgttaacga cgcgctggct gacgatctca aaacctacgg
    11101 tagcgaagtg ttgccgaatc acgaaaacag taaagctatc ttctggaaag agggcgagcc
    11161 gctgaaaaag ggcgacacgc tggtgcaggc gaacctggca aagagcctgg agatgattgc
    11221 tgaaaacggc ccggacgaat tctataaagg cacgattgcg gaacagatcg cccaggagat
    11281 gcagaaaaac ggtggcttga tcactaaaga agatttagcg gcctataaag cggtcgaacg
    11341 cactccgata agcggcgatt atcgcgggta tcaggtttac tccatgccac cgccatcctc
    11401 cggcgggatc catatcgtac aaatcctcaa tattctggaa aacttcgata tgaagaaata
    11461 cggctttggc agcgccgatg cgatgcaaat catggcagaa gcggagaaat acgcctacgc
    11521 cgaccgctcg gaatatcttg gcgacccgga ttttgtcaaa gtcccgtggc aggcactgac
    11581 caataaagcc tatgccaaat ccattgccga tcaaattgat atcaacaaag cgaagccgtc
    11641 cagtgagatt cgccctggca agcttgcgcc ttatgagagt aatcaaacta cccattactc
    11701 tgtagtggat aaagacggta acgcggtggc ggtgacctat acgctgaaca ccaccttcgg
    11761 tacgggcatt gtcgcgggcg agagcggtat tctgcttaat aatcagatgg atgatttctc
    11821 cgccaaaccg ggcgtaccga acatttacgg gctggtgggc ggtgatgcca acgccgtcgg
    11881 gccgaataaa cgtccgctgt catcgatgtc accaaccatt gtggtgaaag acggtaaaac
    11941 ttggctggtt accggtagtc ctggcgggag tcggattatt accaccgtgc
//
LOCUS       DAZOZD010000123        11886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOZD010000123 DAZOZD010000000
VERSION     DAZOZD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.123"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            115..681
                     /locus_tag="ACHUPB_002724"
     CDS             115..681
                     /locus_tag="ACHUPB_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0759493.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            837..2869
                     /locus_tag="ACHUPB_002725"
                     /pseudo
     CDS             837..2869
                     /locus_tag="ACHUPB_002725"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2846..3190
                     /locus_tag="ACHUPB_002726"
     CDS             2846..3190
                     /locus_tag="ACHUPB_002726"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR0759494.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3205..3936
                     /locus_tag="ACHUPB_002727"
     CDS             3205..3936
                     /locus_tag="ACHUPB_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0759495.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3933..4994
                     /locus_tag="ACHUPB_002728"
     CDS             3933..4994
                     /locus_tag="ACHUPB_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0759496.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5146..6192
                     /locus_tag="ACHUPB_002729"
     CDS             5146..6192
                     /locus_tag="ACHUPB_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0759497.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6189..6980)
                     /gene="nei"
                     /locus_tag="ACHUPB_002730"
     CDS             complement(6189..6980)
                     /gene="nei"
                     /locus_tag="ACHUPB_002730"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0759498.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7016..7750)
                     /gene="pxpA"
                     /locus_tag="ACHUPB_002731"
     CDS             complement(7016..7750)
                     /gene="pxpA"
                     /locus_tag="ACHUPB_002731"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0759499.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7740..8672)
                     /gene="pxpC"
                     /locus_tag="ACHUPB_002732"
     CDS             complement(7740..8672)
                     /gene="pxpC"
                     /locus_tag="ACHUPB_002732"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR0759500.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8666..9322)
                     /gene="pxpB"
                     /locus_tag="ACHUPB_002733"
     CDS             complement(8666..9322)
                     /gene="pxpB"
                     /locus_tag="ACHUPB_002733"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0759501.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9345..10088)
                     /gene="ybgI"
                     /locus_tag="ACHUPB_002734"
     CDS             complement(9345..10088)
                     /gene="ybgI"
                     /locus_tag="ACHUPB_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0759502.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10359..11840
                     /gene="dtpD"
                     /locus_tag="ACHUPB_002735"
     CDS             10359..11840
                     /gene="dtpD"
                     /locus_tag="ACHUPB_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0759503.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2860 a         3165 c         2901 g         2960 t
ORIGIN      
        1 ttttacattt aataaaatta ataatatttt taagaaaaac taaaaacatt cgacaatcaa
       61 ataagatatt cttgcaccga tatacttttt tcttttctgt taaaggactc agttatgttt
      121 aaaggacaaa aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat
      181 gcggctgatg aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt
      241 gaaatacatc aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac
      301 attaatcagt cacatcacag cgatgccgtt gctgtcgacc tgcgcttagt caactgtgat
      361 ctggaaaact ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat
      421 agctcagcga aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc
      481 accggcgtcg gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct
      541 actccagata tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg
      601 atggaacaaa ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc
      661 tacgtgctgg attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat
      721 atttatcagg tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg
      781 tggcgatgcc ttgcgcattg gcggtcgaat tcaaccttaa tgttctcgat aaatcgatgc
      841 gcgaccgcat tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt
      901 ttgttagcgt tgcggtaaat aataaccaaa tcagtaacgg gcaaaagatt aactggcaca
      961 aaaatgacga taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa
     1021 aacctgaagt ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac
     1081 ctgaaatgct cttcaatttc gatcaagcca atcagcaact gaatattagt attccgcaag
     1141 cctggctggc gtggcactca gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg
     1201 ccggtatcct gatggattac aacttgtttg ccagcagcta ccgcccacag gacggcagca
     1261 gcagcactaa cctgaacgcc tacggtacca ccggaattaa cgccggggca tggcgcttac
     1321 gtagtgatta tcagttgaat cagactgata gcgatgataa ccatgaacag tcaggcggaa
     1381 tatcgcgcac ctatcttttt cgtccattac cgcaattagg ctctaaatta accctcggcg
     1441 aaacggattt tagttccaat attttcgacg gtttttctta taccggcgcg gcactggcaa
     1501 gtgacgagcg aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg
     1561 cacagaccaa tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag
     1621 tcccaccagg cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg
     1681 tcaaagtgac ggaagaagat ggtcgggtga acaatttcca ggtttcggca gcatcgacgc
     1741 ccttcctgac tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct
     1801 ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc
     1861 tgtcaaacac ctcgctgtac ggcggcctgc tgctttctgg tgatgactac cattctgccg
     1921 caatgggtat tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg
     1981 ccagtagcca ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca
     2041 gcaaacaagt ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc
     2101 gtcattttca cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg
     2161 acgaaaaaca gacgatcagc ttattgtggg ccaaccgatt accccactaa acctcaatct
     2221 ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga ccgccaacat
     2281 cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt cgacgtcatt
     2341 caatacaacc cattacgaag ataaagatca cgacaaccag atttacctgt cgatttcgct
     2401 ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc acagcaccac
     2461 acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca tgtctgccgg
     2521 actgcaatcc gaccgtcctg acaatggagc ccaggtgagc ggtaactatc agcacctgag
     2581 ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca gttccgtcag
     2641 cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc atcgccgcag
     2701 ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata ttccggttca
     2761 gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga tttccagtta
     2821 ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa cagttgcaga
     2881 aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac tggcttcccg
     2941 ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc ctcccctcgg
     3001 agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg aggaaggaca
     3061 tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg gtgatagcca
     3121 gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc tgattttacc
     3181 ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct tttgttgttg
     3241 gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat tgtgtttaac
     3301 gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact cccctacctc
     3361 gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct ggtcgccctg
     3421 ccgccgatac aacgtctgga accgaaagca acaacgcagg tacggatagt aaagcaagcc
     3481 tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat gcgtgaaatt
     3541 cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat tcagagtcgt
     3601 atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa ggtcgaactg
     3661 cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac cgcgtattat
     3721 ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt caaaaccgtg
     3781 atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg cagccagttt
     3841 tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa ctgcagcgga
     3901 caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga gtgttgctcg
     3961 tcatctgtct gttatttctg ccgttgaagt ctgcgcttgc cctgaactgc tactttggtt
     4021 catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta ccaggcaatg
     4081 ccaaactcgg cgataagatt tgggaatctg acgatattaa aattcccgtc tattgtgaca
     4141 acaataccaa cggcaatttt gaaagtgagc atgtttatgc ctgggtaaac ccctacccgg
     4201 gggtgcagga tcgctattat caactcggcg taacttacaa tggcgttgat tatgacgcca
     4261 accagggaaa aagccgcatc gacactaacc agtgtattga cagtaagaac attgacattt
     4321 atacccctga gcagattatc gcgatgggat ggcagaacaa aatttgctcg ggtgatcccg
     4381 ctaatattca catgtcgcgt acctttctcg ctcgcatgcg gttatacgtc aaaatacgag
     4441 aaatgccgcc gcatgattat caaagtacgc ttagcgacta tatcgtcgtg caatttgacg
     4501 gtgccggtag cgttaacgaa gaccctactg cccaaaacct gaaatatcat attactggtc
     4561 tggaaaacat tcgcgtgctg gattgcagcg tcaatttttc catttcaccg gaaacacagg
     4621 tgattgattt tggtaaattt aatttgctgg atattcgtcg ccacacaatg tcgaaaacat
     4681 tcagtattaa gacgacaaaa tcacaaaatg accaatgcac cgacggattt aaagtcagtt
     4741 cctcctttta taccgaagaa acgttggttg aagaagataa agcattgttg attggtaatg
     4801 gtctgaagct acgtttgctg gatgaaaacg cctcgcctta taccttcaat aaatatgctg
     4861 aatacgccga tttcaccagc gacatgttgg tctacgaaaa aacctataca gctgaacttt
     4921 cgtccattgc aggtaccccc atcgaggctg ggcccttcga tacagtggtg ctttttaaaa
     4981 ttaactataa ctgatttgcc atgatgccct ttgcagccgg aaagggcatc tctttcagaa
     5041 aattgctgat tttccccttt tccttgattg ctgttatttt ttcctggccc ggctttcttt
     5101 atgctgtatc gatacaccaa agagaaaata atgagggagc gaaggatgcc agttttgcag
     5161 tggggaatgt tatgtgtgct atcacttctc ctttctattg gtttcctcgc ggtacatctc
     5221 ccggcggcgc tattacttgg gccgatgatc gccgggatca tcttcagtat gcgcggaatt
     5281 actctgcaac ttccccgctc cgcttttctc gccgcgcagg ccattcttgg ctgcatgatt
     5341 gcgcaaaacc tcactggttc gattctcacc acgctggccg ccaactggcc gatcgtgctt
     5401 gctattttac tggtgacgct gctttccagc gccatcgtgg gttggttatt ggtgcgctat
     5461 agctcactgc ctggaaatac cggtgcctgg ggttcttctc caggcggcgc agcggcaatg
     5521 gtcgctatgg cgcaagatta cggcgcagat attcgcctgg ttgcatttat gcaatatctg
     5581 cgagtgctgt tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct gggtgataac
     5641 gctgaagcgg ttaaccagca aatcgtctgg ttcccgccag tgagcattaa tctcctgctt
     5701 acgattttgc tcgcagtcgt tgccggtacg gcgggatgca tgctgcgtct tccttccggc
     5761 acgatgctca tcccgatgct ggcgggcgca gtgctccagt ctggtcagct catcaccatc
     5821 gaactaccgg aatggctgct ggcgatggcg tatatggcaa ttggctggcg gattggtctt
     5881 ggtttcgata aacaaatctt actgcgggca ctacgcccgc taccgcaaat cctgctgtcg
     5941 atttttgctc tgctggctat ttgtgcgggt atggcgtggg ggctgacccg gtttatgcac
     6001 attgatttta tgactgccta cctcgccacc agtcccggcg ggcttgatac cgtggcggtc
     6061 atcgccgcag ggagcaatgc cgatatggcg cttattatgg cgatgcaaac cctgcgcctg
     6121 ttcagtattt tgctgacggg gccagccatt gcgcggttta tttcaaccta tgcgccgaag
     6181 cggtcggcct agtgctggca gccagggcac cagtaaaacg ggcgagatga cagcgtggtt
     6241 ttctcaatga tgctgccaca gcgttcgcac ggttcgccat cacgatgaaa aaccttaaag
     6301 cgaaacagcg ccccatgatg cttattctca tccaccagcc ccctcgtagc gtaggaaaat
     6361 cgaggaatat ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc
     6421 gctttatgat ttccagtcaa cccaacctgc cagaggatct ccacccgcaa ataattgcca
     6481 agcccagcca gaaacgcctg atcgagcagt aatcctgcaa actgacggtt acgaaagcgc
     6541 ggtgacaata accgttcctt taccacctcc ggcgtcagat tcggatccag cacatcggga
     6601 ccaacgcgtt gtaaaaacgg atgcgtggtc agttgttccg gcctcaacat ctcaatatcc
     6661 gaggcgctat aaagcagaat ggttttgtca gccgtttgca gttttacccg caatactcgc
     6721 gtggtctgcg gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg
     6781 ctgtagagcg ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg cgtttccaca
     6841 tgggtaacgt gttgaccgat aagttgtgat tgataagttt ttaactgcgg gaaggcaaac
     6901 cagacatcag ttagtggttt gcctttgatc gccgcctcca ggttatccgc tgcacggcgg
     6961 atctccgggc cttcaggcat ggtgatatcc ttttttcatt ctttattaat cggggttatg
     7021 ctgcgacaac aatctccttt tcggcaaatg tagagcgtag tcggcgggcg aacgccagtg
     7081 cgtgctcgcc gtcgccatgc aggcagactg tttgcgccgt gaccgttgcc cattcgccgg
     7141 tgatactttt gactctgccg tgttgcacca tctctagcgt ttgcgccaac gcctgttctt
     7201 cgttttcaat caacgcgcct cgctgacttc gcggcaccag cgagccgtca gcctgataac
     7261 cgcgatcggc aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct gcacgaatca
     7321 gctcgcttcc tgccagccca acgagaatca atgccggatc gcaagcgtat accgctctgg
     7381 cgatggcgtc tgccagttgt gcttctttcg ccgcctggtt gtacaacatg ccgtgcggtt
     7441 tgacatgacg cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg ccaatttgat
     7501 acagcgtctg ggcgtaaacg gtttctggcg gcagctgcat ggcgctgcga ccaaaatttt
     7561 ccctgtcggg aaaactcggg tgagcgccaa tcgcgacacc attttttatt gcttcacgca
     7621 cgcaagcctg catgatttgc gcatcgcctg catgaaatcc acaggcaata ttggcagagg
     7681 aaaccagcgt taatagctct gcgtcgctgg cgcagccttc gcccagatcg gcgttcaggt
     7741 caattttcat tgtgcagccg ccacactaat tgttcgaaat aacgttgctg atcttgccgc
     7801 gctttcagtg cctcttccag tgaacactgg acaaaatgaa tcggctgacc gagcggaatt
     7861 tgcgccagat ggtacatatc agcctcaatg atacaggcaa tacgcgggta accatcggtg
     7921 gtctgtgcgt cgttcatcaa cacaatcggc tgcccgttat gtggcacctg caccacgccc
     7981 ggtaacaaac cgtgagataa cagttcgcga tcggtggtgc gttttaaaat ttgcccctgt
     8041 aagcgatagc ccatgcggtt actttgcgag ctaagctgcc agggcgaacg ccagaatgca
     8101 tcctgcgagg cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg aatgcggttg
     8161 ccccacagca gctgtttaac gccctgcgct tccataaaat cacgcttcga tttgccaatc
     8221 gggagtcggt caccatcctt cagtaaacgg ccttccagcc cgccaatccc cactttgaga
     8281 tcggtgctgc atgaccccat taccggcgga acatcaatac cacccgcgac cgccagataa
     8341 ctgcgcatcc cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt catcggcaat
     8401 cgccagccgg tccagacggc attatcatcc agccgcgctt cgcaaccggc acccgtcaga
     8461 gcaaaccacc catcagtttc gaactcaacc gttaactgac cgagcgtgat ctccagcgcg
     8521 ggggcattgg cgtcattacc caccagcagg ttagcaatgc gtaacgcagg catatccagt
     8581 gcgccgcagt ggctgattcc cgactggcga aaaccgtgac gaccgccatc ctgcacagtg
     8641 gtatacatgc ccgcacgaat aatcttcagc atactccctc cttctgcggt acaaagcgca
     8701 cgctgtctcc cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac aggctgagtg
     8761 aggtatgacc aatcaactgc cagccacccg gcgttgccag cggataaaca ccagtctgcg
     8821 gcccgccgat cccgacagaa cctgccggaa cgagcaagcg cggttcagcg cgccgtggcg
     8881 tgtgtagttg ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc
     8941 agaccacgta ttccacggag gagtgcaatt caacaacctg tttttcgcta aacccgcaat
     9001 gcgccgcgac caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc ggaatttcaa
     9061 taaagcgaga ctcaggttcc agggcctcgc tctcctccca ccagcgttgc aaacgctcta
     9121 tggcatccag cgccagcgac tcaggattac gcaaaatcac cgtgatattg ttcatgccgg
     9181 gaatggcttc aaccacattc ggcatatcca ccagacgctg cgccagtcgc cagatccgtt
     9241 tctggctagc cagcgtcacc ggcggttcca gttccagcac taccgccgtt tcacctatca
     9301 gataacaacg cgctcgttgc actttcgatc cctctgatta ttcgttatgc aggattagga
     9361 atatcaataa aggtcacatc aagatcggta ttttcattca gccactcgct caatgcgcga
     9421 ataccaccac gttcagtggc atggtgacct gcagcataaa aatgcaaccc ctgctcgcgg
     9481 gctgaatgaa tggtctgttc agaaacttcg ccagtaataa aagcatccac gccaaaacgc
     9541 gcggcgctat cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg ctggaccacc
     9601 tcaggtccgg tatcgccaca ccataacggc ttacgtccca gacgcgcttc aatccaggag
     9661 gctagttcca gtcccggcac aggcatggtc agctctcccc acggcaccaa cggctcaatt
     9721 tcgcccatga ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc cagctcagga
     9781 tgtacgtcaa gcggcaaatg ccagccatac aggttgatat cattcgccag caacgttttt
     9841 aaacggttac gcttcattcc gcgaatgacc ggagactctc ctttccagaa gtagccatga
     9901 tgcacaatga ccgcatccgc gcccagacgc actgcctcat cgagcaaagc ctggctggcg
     9961 gtgacaccgg taacaatttt ttgcaccgtc tctttgcctt ctacctgcaa accattcggc
    10021 gcgtaatcac taatcgccgc gctgttcagt ttttcgttaa tcagttgttc cagttcggtg
    10081 tttttcatct tcgcgccttc cgtattctga tagctacacc gttaacatag cgttacctcg
    10141 cctgttcgtc gatagccaat cttttgacat attaagaaac aaacccgacg ccaaaagacg
    10201 caaattgttt atatcatcac gcttcagcta atgtatattt atgcaatcag acagcatatt
    10261 gcataaataa tcagatctgt atcaaagttt taactttaag gaaacacaag cacgccgcgc
    10321 aactattacg tgacgttgca gtcaattgtg gaattattat gaataaacac gcatcacaac
    10381 cgcgcactat ttactatgtc gttgcgctgc aaatctggga atattttagc ttttacggca
    10441 tgcgtgccct gctgattctc tatctcacca atcaactaaa atacaacgat actcacgcct
    10501 acgagttatt tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc ggtggctttt
    10561 tggcggataa agttctcggc aatcgcatgg cggtgatgct gggggcgttg ttgatggcga
    10621 tcggtcatgt ggtgctgggt gccagtgaga tccatccgtc attcctctat ctgtccctgg
    10681 cgattatcgt ttgtggctat ggtctgttta aatccaatgt cagttgcctg ctcggcgagc
    10741 tgtatgagcc aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat gcagcgggta
    10801 acgtggggtc tattatcgca cctatcgcct gtggttacgc ccaggaagag tacagctggg
    10861 cgatgggctt tggcctggcg gcggtgggta tgatcgcggg tctggtcatt ttcttatgtg
    10921 gcaatcgtca tttcacacat acccgcggcg ttaataaaaa agtactgcgt gcaacaaact
    10981 ttctcctgcc taactgggga tggctgctgg ttctgctggt ggcaacgcct gcgctgatta
    11041 ccgtactgtt ctggaaagag tggtcggtat acgccttaat tgtcgcgact attattggcc
    11101 tgggtgtact ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgt aaggagctgg
    11161 ggctgattgt gacgctcacc ttcttcagta tgttgttctg ggccttcgca caacagggcg
    11221 gcagctcaat tagcctttat atcgaccgct tcgttaaccg cgatatgttt ggttataccg
    11281 ttccgactgc tatgttccag tcgattaatg ccttcgcagt tatgctatgc ggtgtgttcc
    11341 tggcgtgggt ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc tggggaaaat
    11401 ttgctcttgg ccttggcctg atgagcgccg gattctgcat tctgacctta agcgcccgct
    11461 ggtccgcaat gtatggtcac tcttctctgc cactgatggt gttaggcctc gcggtgatgg
    11521 gctttgcgga actgtttatc gacccggttg ccatgtcgca aattacgcgc attgaaattc
    11581 cgggcgtgac cggcgtatta accggcatct acatgctgct ttctggcgcg atcgcgaact
    11641 atctggcggg cgtgattgcc gatcagacat cgcaggcttc gtttgatgct tccggggcga
    11701 tcaactactc catcaatgca tatattgaag tatttgatca aattacctgg ggcgcactgg
    11761 cgtgtgtagg actggtactg atgatttggc tgtatcaggc gctgaaattc agaaaccgcg
    11821 cgctggcgct ggagtcataa taggccagca ccgatgcctg atgcgacgct tgacgcgtct
    11881 tatcag
//
LOCUS       DAZOZD010000124        11816 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOZD010000124 DAZOZD010000000
VERSION     DAZOZD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.124"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUPB_002736"
     CDS             <1..389
                     /locus_tag="ACHUPB_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0759504.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            complement(588..938)
                     /locus_tag="ACHUPB_002737"
                     /pseudo
     CDS             complement(588..938)
                     /locus_tag="ACHUPB_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHUPB_002738"
     CDS             complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHUPB_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0759505.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHUPB_002739"
     CDS             2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHUPB_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR0759506.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2916..3704
                     /locus_tag="ACHUPB_002740"
     CDS             2916..3704
                     /locus_tag="ACHUPB_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR0759507.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3910..4257
                     /locus_tag="ACHUPB_002741"
     CDS             3910..4257
                     /locus_tag="ACHUPB_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0759508.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4251..5090
                     /gene="sul1"
                     /locus_tag="ACHUPB_002742"
     CDS             4251..5090
                     /gene="sul1"
                     /locus_tag="ACHUPB_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0759509.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5218..5421
                     /locus_tag="ACHUPB_002743"
     CDS             5218..5421
                     /locus_tag="ACHUPB_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759510.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5577..6782
                     /gene="chrA"
                     /locus_tag="ACHUPB_002744"
     CDS             5577..6782
                     /gene="chrA"
                     /locus_tag="ACHUPB_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR0759511.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6793..7098
                     /locus_tag="ACHUPB_002745"
     CDS             6793..7098
                     /locus_tag="ACHUPB_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR0759512.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7325..8089
                     /locus_tag="ACHUPB_002746"
     CDS             7325..8089
                     /locus_tag="ACHUPB_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR0759513.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHUPB_002747"
     CDS             complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHUPB_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR0759514.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHUPB_002748"
                     /gene_synonym="mrx"
     CDS             complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHUPB_002748"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR0759515.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10401..11306)
                     /locus_tag="ACHUPB_002749"
     CDS             complement(10401..11306)
                     /locus_tag="ACHUPB_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0759516.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11428..>11816)
                     /locus_tag="ACHUPB_002750"
     CDS             complement(11428..>11816)
                     /locus_tag="ACHUPB_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0759517.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2490 a         3391 c         3450 g         2485 t
ORIGIN      
        1 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
       61 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
      121 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
      181 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
      241 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
      301 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
      361 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
      421 cgctaacttt gcaacagtgc ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg
      481 tcagggaaga ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat
      541 cctgacgcaa tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc
      601 ggcgaatcac aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg
      661 aagcgctgat agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg
      721 aaggcggcat cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg
      781 gcgttcatgg tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc
      841 ggcctggcga acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg
      901 cagtcgggtg tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg
      961 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
     1021 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
     1081 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
     1141 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
     1201 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
     1261 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
     1321 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
     1381 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
     1441 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
     1501 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     1561 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     1621 caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc
     1681 cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag
     1741 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     1801 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     1861 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     1921 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     1981 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2041 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     2101 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2161 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2221 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2281 taaaacaaag ttagccatta agggagttaa attgaaaata tcattgattt ctgcagtgtc
     2341 agaaaatggc gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact
     2401 actctttaaa gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa catttgactc
     2461 tatgggtgtt cttccaaatc gcaaatatgc agtagtgtca aagaacggaa tttcaagctc
     2521 aaatgaaaac gtcctagttt ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt
     2581 tacagatcat gtatatgtct ctggcggggg tcaaatctat aatagcctta ttgaaaaagc
     2641 agatataatt catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca aattccctat
     2701 aatgcctgag aatttcaatt tggtttttga acagtttttt atgtctaata taaattatac
     2761 ataccagatt tggaaaaaag gctaacaatg cgttgcagca ccagtcgctt cgctccttgg
     2821 acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa
     2881 cttaaaagct gccgctgaac ttaacgttag gcatcatggg tgaatttttc cctgcacaag
     2941 ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg
     3001 acacaatcca cctgttcgga tctgcgatcg atggagggct gaagccggac agcgacatag
     3061 acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggcg ctaatgctcg
     3121 atttgctgaa agtctcatca ccgccaggcg atggcggaac atggcgaccg ctggagctaa
     3181 ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt
     3241 tcggtgagtg gctccgccac gacatccttt ccggaacgtt cgagcctgcc gttctggatc
     3301 acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgcgctt ctaggcccat
     3361 ccgcagccac gtttttcgag ccggtgccga aggagcattt ctccaaggcg cttttcgaca
     3421 ctattgccca gtggaatgca gagtcggatt ggaagggtga cgagcggaac gtcgttcttg
     3481 ctcttgctcg catttggtac agcgcttcaa ctggtctcat tgctcctaag gacgttgctg
     3541 ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg
     3601 cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt
     3661 tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa
     3721 gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc ggacgggctg
     3781 cgcccgccgc tcaactatgc gttagatgca ctaagcacat aattgctcac agccaaacta
     3841 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     3901 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     3961 acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata
     4021 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
     4081 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
     4141 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
     4201 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
     4261 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
     4321 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
     4381 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
     4441 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
     4501 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
     4561 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
     4621 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     4681 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     4741 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     4801 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     4861 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     4921 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     4981 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     5041 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     5101 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     5161 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     5221 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     5281 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     5341 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagggatc
     5401 gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc
     5461 cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat atcgaatttc
     5521 gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc gaaagtatga
     5581 cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt ctgcggcttg
     5641 gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc gaatttgtca
     5701 cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt tgtcagttct
     5761 tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg gctggataca
     5821 gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc ttgatccttt
     5881 ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg catggcttaa
     5941 aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt aacctatgca
     6001 cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt
     6061 ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg ttattgttca
     6121 agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac cgggccggcg
     6181 tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg gccgaactga
     6241 tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca ctggtgttcg
     6301 gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc ggctgggtca
     6361 acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc cctttgttca
     6421 cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg atcggcggca
     6481 ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat
     6541 tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg atcaatgccg
     6601 ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc gccatctttc
     6661 agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc tggaagctcc
     6721 cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt
     6781 gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct gcacatcctt
     6841 caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct acgccgacac
     6901 ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc
     6961 tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta tgacatcact
     7021 gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct gttcggagag
     7081 ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc ctaactggat
     7141 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
     7201 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
     7261 gtgggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     7321 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     7381 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     7441 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     7501 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     7561 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     7621 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     7681 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     7741 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     7801 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     7861 gatactgatc aagccggtgc gcggtttcaa atcgatcccc acggcctatg ccacgatcaa
     7921 gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc
     7981 cggcatcagg ggcgaggtgc gccttgtgga gagagctttt ggcattgggc cctcggcgct
     8041 gacggaggcc atgggcatgc tcaaccacca tttcgcagca gccgcctgat cggcgcagag
     8101 cgacagccta cctctgactg ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct
     8161 cggtgatggt ggagtgaagc caacccgcaa tcgggttatg aatctgcatc gcgattcgca
     8221 atcagctgtc tcttgagcat gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc
     8281 tacaccaact tgtccaactc atccgccaca ccgctcccca ccacctgcga tggccaggtt
     8341 gtttgctggg cgcattggcg gagtatcgtc tcgacccggg acacccacac cggcacaacc
     8401 ttacgggagt gattcactgt caaagaatcg gcccggtgct ctgacgcaag tatcgggatg
     8461 gtcaccattt gtaagccgta gacctgagtg gtgatcaaga cttcgatacc accgaccgta
     8521 ccggtactaa tcgacgacgg tcgtgttcgt cgcctgccgc agggactctg cacacctccg
     8581 tttacgcatg tgcctggagg agttggaaat cgtcgtgttc gggaaacatt aaacacagga
     8641 tggcagcgat ctgagccagc acatgatcag ctagctcacc atccggatcg acggcccact
     8701 gcatcgtcgc gccagcgatg accgagtgca ggagcaactc agctgccgca ggagcacctg
     8761 ggggcagtcg cttgcggatc ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc
     8821 gtagctccgg cacctggagc tcgtaccagg agatgagata gttcaccgag aagtcgttgc
     8881 gagtgttcat gctccgaacg agcacctgca aaaattccca gagcccttgc ggccctgcgc
     8941 ctatcggtat cgcattcagg taatgccgca cctgctcgac gccgcgctcc atcatcctca
     9001 ccagcagcgt atcgcggttg gtgaagcgct ggattaacgc tgcgcgggag agccccacct
     9061 cctttgctac tccgctgagc gtgaactcta tgggaccgca acgcttcagc actacggtgg
     9121 cggcctcgag tacctcgtca tcggacttga gcttggggcg gggcatcagt gttcaccttc
     9181 tgtatgggtt ggggcggagg ctgtggctgc cgccgccatt gtagcaaatt gaagacggag
     9241 cgagagtaga gccacgagcc ccgcaaacac ggccgataca acgaggccag ggagcggacc
     9301 agcaaggtcg acaaacgcgc cggccgcaag cataaccatg ggcgaggctg acagcatcac
     9361 cgccgagacc gtgccgagta cccggccgag aagttctggc ggcgtgcggt tgtagatggc
     9421 agcgttgaga atgggagaga ctgagccggt cagcagtccc acgagcgcgc ccaacaacat
     9481 cagcaccggc acgcctggca actgtgaaag cagaagcgag cccaccgcag agccacaaaa
     9541 tgccaccgcc agccagttct gcgctgatat ccgggcgccg accgacgcat gaatggcaat
     9601 gccaaggaga ccaccagccc ccatcattga ggagaacagc ccgagctctg ctacttggcg
     9661 tcctgcatct acaaacagcg caggcatgat gacgctgccg ttggcgccaa cgatgcccac
     9721 gaagatcatc actataccaa agagagggcg cagcaggggt tcgctccaga gaaaagcgac
     9781 gccggcgcgc atggagagag tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag
     9841 cacccacgcg ccgagcagac ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg
     9901 cgcagcgcca agcaggccga ttgcggcccc cccaagggcc gggccaccta gaatcgcgac
     9961 gttcccgatc accgctttca gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc
    10021 gagtttgggc agctcactgt cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag
    10081 caccacgcaa gcgatcagcc cagtgttcga gagggcgcca acggcatcga gcagtgggat
    10141 gctcgccatg gccacgccgc ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa
    10201 ccgatcgacg aggccaccac caacccacgc gccgatgatg gtcgcgatga cgctgctagc
    10261 ggccgtggcg cccgcccagg ccgcgctctt tgtatgagac aggacgaacc atggaagcgc
    10321 gagggccgcc accgcgttgc cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag
    10381 cgggctatat cgacgttcgc tcattccgct gcggcgagct gcgccttcgc cgcagcgagg
    10441 tactcttcgt tacccgagtc gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg
    10501 cgctccgcga tgtggtgggc gagccgcggc cacacccggc caccggccgc ttcatacgtg
    10561 aggaggagct tcgcgagccc ctcttcacca aagaccataa ggtgcgcggc catgtcgatg
    10621 gcagggtcat caacgcgggc ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg
    10681 tcgatgagca catggcccac gtagagatcg ccatgcacca ccacggagaa atctggccac
    10741 gacgaatcgt cgtcgagcca gcgctgccac cggtggaggc gcttgtcgtt caccacgaac
    10801 tcgcgtcgga cgcggtcaac gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg
    10861 atgagcatcc ccgcatccac ggcggcggaa atggggacgg catgcagggc ggcgagcgcg
    10921 gtcgcgaagc tctccgcgaa gacctccgag tcctgcggca cgacccagtc gggcgtggac
    10981 gaaccaggct ggatgaccat cgcagtcgag tcttcgagca tgggataggc aacgagctcg
    11041 gcgttggcca cgcgccagtc cggcaccgcg aacggcaggc gattcttgag cattgccagc
    11101 acccgcgcct ctggttcgac cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc
    11161 caccgacgtc catcgtcgac ggtggcgatc acgatcctat agtcgagccc aagctcattg
    11221 acagtcagcg ggccatggag cttgagccca tgtcgggctg caagtgcgta cagttgggag
    11281 gtatcggcgg tcgtgactac ggtcatgatt cactcctgag ggcttgacgg gtttagccac
    11341 ctaaatgtaa cagtcacgtc ggttatattc aatccggcac tgttgcaaag ttagcgatga
    11401 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
    11461 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
    11521 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
    11581 gccgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
    11641 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
    11701 accataggcg ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt
    11761 gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg aggaga
//
LOCUS       DAZOZD010000125        11807 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOZD010000125 DAZOZD010000000
VERSION     DAZOZD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.125"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..487
                     /locus_tag="ACHUPB_002751"
     CDS             <1..487
                     /locus_tag="ACHUPB_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0759518.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUPB_002752"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="ACHUPB_002752"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0759519.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUPB_002753"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHUPB_002753"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0759520.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUPB_002754"
     CDS             complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHUPB_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0759521.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUPB_002755"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHUPB_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0759522.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUPB_002756"
     CDS             complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHUPB_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0759523.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUPB_002757"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHUPB_002757"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0759524.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUPB_002758"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHUPB_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR0759525.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6838..7746)
                     /locus_tag="ACHUPB_002759"
     CDS             complement(6838..7746)
                     /locus_tag="ACHUPB_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0759526.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUPB_002760"
     CDS             7922..9232
                     /gene="abgA"
                     /locus_tag="ACHUPB_002760"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR0759527.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUPB_002761"
     CDS             9232..10677
                     /gene="abgB"
                     /locus_tag="ACHUPB_002761"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR0759528.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10714..>11807
                     /locus_tag="ACHUPB_002762"
     CDS             10714..>11807
                     /locus_tag="ACHUPB_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0759529.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3005 a         2999 c         2927 g         2876 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg atattaataa
     6661 tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa aggttaaaac
     6721 cgttataaca cagtcaccgg cgcagaggag acaatgccgg atttaagacg cggatgcact
     6781 gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta tgcagtttta
     6841 actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg tcagtggtgt
     6901 ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa tttcgctaac
     6961 cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt caggcagtat
     7021 gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct cgcagactac
     7081 accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt tgtagtaact
     7141 gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg aacgggcacc
     7201 aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta atttctcaaa
     7261 agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat ccaattctcc
     7321 ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta cttttacctg
     7381 cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg aaatactggc
     7441 ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa tatcctcttg
     7501 ggctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt caccggcatc
     7561 agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc cttcttccag
     7621 ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc tcgctccgcg
     7681 aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt ttacctgaaa
     7741 agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca tctactttaa
     7801 tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa tcagttagtg
     7861 ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga cgggaaaggt
     7921 tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc actggcgacg
     7981 tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca cccttgttgc
     8041 ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag ttaatgaaag
     8101 tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc gcgctcgtca
     8161 acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcactg gtatcgtcgc
     8221 caccctggat accggtcgcc ccggtccggt gatggctttc cgtgtcgata tggacgcgct
     8281 ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt ttgcgtcatg
     8341 taacgccgga atgatgcatg cctgtggtca tgatggacat accgccattg ggcttgggct
     8401 ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac tgatttttca
     8461 gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg tcgtagatga
     8521 tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca ccgtggtgtg
     8581 cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg gtaccgccgc
     8641 tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag cacaagccac
     8701 tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa acgtgggcgt
     8761 tatgcaggca ggaagcggtc gtaacgttgt tcctgcctcg gcgttgctga aagtggaaac
     8821 acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac aagcgattca
     8881 gggcgcagca accatgtatg gtgtcggcgt tgaaactcgt ctgatgggtg cagttaccgc
     8941 cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc aggtcgcggg
     9001 ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg ccacattaat
     9061 gatggcccgc gtgcagcaac atcaagggca agcctcctac gtggtgtttg gcacacagct
     9121 ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg ctattgccgt
     9181 cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct gatgcaggaa
     9241 atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac cgatattgcc
     9301 gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc ggagcatctg
     9361 gcttcggctc tggaatctgc aggcttcacc gttacccgca acgtaggcaa tatcccaaat
     9421 gcctttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg agaatatgac
     9481 gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc gacgcccggt
     9541 gaaaatggtc acggttgcgg acacaatttg cggggaaccg ccgcctttgc cgctgcaata
     9601 gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt ttatggttgt
     9661 cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt atttgatgat
     9721 gtggatgcgg cactcacctg gcacccggaa gcctttgccg gtatgttcaa tacccgcacg
     9781 ctggcaaaca ttcaggcatc atggcgcttt aaagggatcg cagcacatgc cgcgaattcc
     9841 cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg caccaacttc
     9901 ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa tagcggcggg
     9961 atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg cgcccccgaa
    10021 atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg tgcggcattg
    10081 atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta tctcccgaat
    10141 cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc ggaatggaac
    10201 tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa cgatcggcaa
    10261 aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt tgcactacgt
    10321 catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga taacgtgctt
    10381 gcggcatcga ctgatgtcgg cgacgttagt tggaaactgc ctgttgccca gtgtttcagc
    10441 ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca ggggcgaaca
    10501 tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac cacagtcaat
    10561 ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca agtaacggac
    10621 acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt aaaataacaa
    10681 caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat accgtcgtcc
    10741 tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt tctccatcct
    10801 tttttgctct ttatctattt gattatcgta ctcatggtga cgacggcaat tttgtcggcc
    10861 tttggcgtca gtgcgaaaaa cccgaccgat ggaacgccgg tggtggtgaa aaacctgctc
    10921 agtgtggaag gattacactg gtctttaccc aatgttatta aaaactttag cggttttgct
    10981 ccacttggtg cgatcctggc gctggtttta ggtgccggtc tggcggagcg cgtcggctta
    11041 ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc cagttatatg
    11101 gtgctgttta ttgctttttt cagccacatt tcttccgatg cgacgttagt gatcatgcta
    11161 ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg tttactggct
    11221 gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac aaccgacgtg
    11281 ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat gcacgtcagt
    11341 gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat tgttggcggc
    11401 ctgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa cagcgatgag
    11461 aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg tgtcgtatcg
    11521 ctacttttta ttgctgcgat tgcgctgatg gtgatcccgg aaaacgggat attgcgcgat
    11581 ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc actgatcatt
    11641 ctttttttct ttgttgtctc gctggcttat ggcatcgcta cccgcacaat tcgacgtcag
    11701 gcggatttac cgcagttaat gattgaaccg atgaaagaga tggcaggatt tatcgtgatg
    11761 gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacat
//
LOCUS       DAZOZD010000126        11612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOZD010000126 DAZOZD010000000
VERSION     DAZOZD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.126"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUPB_002763"
     CDS             <1..347
                     /locus_tag="ACHUPB_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0759530.1"
                     /translation="LYGCLLSLYSICARDIYNMSAGNNVKVAADAFLEIIFSLKNKYC
                     IKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRYIPQSQDGEDFR
                     SHLQYKLPLSAH"
     gene            558..881
                     /locus_tag="ACHUPB_002764"
     CDS             558..881
                     /locus_tag="ACHUPB_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0759531.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            878..1708
                     /gene="amiD"
                     /locus_tag="ACHUPB_002765"
     CDS             878..1708
                     /gene="amiD"
                     /locus_tag="ACHUPB_002765"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0759532.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1705..2718)
                     /locus_tag="ACHUPB_002766"
     CDS             complement(1705..2718)
                     /locus_tag="ACHUPB_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0759533.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2817..4247)
                     /locus_tag="ACHUPB_002767"
     CDS             complement(2817..4247)
                     /locus_tag="ACHUPB_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0759534.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4258..5259)
                     /gene="ltaE"
                     /locus_tag="ACHUPB_002768"
     CDS             complement(4258..5259)
                     /gene="ltaE"
                     /locus_tag="ACHUPB_002768"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0759535.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5296..7014)
                     /gene="poxB"
                     /locus_tag="ACHUPB_002769"
     CDS             complement(5296..7014)
                     /gene="poxB"
                     /locus_tag="ACHUPB_002769"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0759536.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7147..8115)
                     /gene="hcr"
                     /locus_tag="ACHUPB_002770"
     CDS             complement(7147..8115)
                     /gene="hcr"
                     /locus_tag="ACHUPB_002770"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0759537.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8127..9779)
                     /gene="hcp"
                     /locus_tag="ACHUPB_002771"
                     /gene_synonym="priS"
     CDS             complement(8127..9779)
                     /gene="hcp"
                     /locus_tag="ACHUPB_002771"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0759538.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9923..10822)
                     /gene="lysO"
                     /locus_tag="ACHUPB_002772"
     CDS             complement(9923..10822)
                     /gene="lysO"
                     /locus_tag="ACHUPB_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0759539.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11317..>11612)
                     /locus_tag="ACHUPB_002773"
     CDS             complement(11317..>11612)
                     /locus_tag="ACHUPB_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR0759540.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         2824 a         3073 c         3079 g         2636 t
ORIGIN      
        1 atttgtatgg ctgtttacta tctctgtact ctatctgtgc aagggatata tataatatga
       61 gcgccgggaa taacgtgaag gttgctgcgg atgctttcct tgaaattatt ttctctctta
      121 agaataaata ttgcattaaa ctattaagcg ctcaggaacg ggcgtttatt tacgaatttg
      181 caagaatgat cttcgcttat attaatgaca aaagcattga gatcttatta ctttcttgtt
      241 ttgcggcgtc tgaccaaaaa gcaatccaga ggtatatacc tcagtcacaa gatggggaag
      301 actttaggag tcatttacaa tataagttgc ctctttccgc tcattaatgt tgttggtgca
      361 acataataag acaggtaagc ggtaccctat aatgaaacca atatcgggtt atgcaacgga
      421 taatcgtaaa gacttaagtg ggtgtttttg acacaatgag atgatttcct aaactgatca
      481 aaaccttctt cccaacatgg catatcagtt cgataatgct ttagagatcg aaggcttaac
      541 aaacaaggag atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg
      601 ttgaatattg cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt
      661 tctttgccgg tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc
      721 gtaaagcacg ggagatcgcc tttgaggaat taggctccca ggcgcgggcg ctgggggccg
      781 atgccgtcgt cggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga
      841 tggttagcgt cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctggt
      901 cgctgccgct ctgttattgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg
      961 atatcagctt gatacccgac gccaggcgca ggcggcgtat ccgcgcatta aagtgctggt
     1021 gatccactac accgcagatg attttgatag ctcgctggcg acactgaccg ataagcaggt
     1081 cagctcgcat tatctggtcc ctgcggtacc accgcgatac aacggtaaac cgcgcatctg
     1141 gcaactggtg ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc
     1201 aacgcgcctt aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc
     1261 ggccggagtg aaatattttg ccccgtttga accgacacag attcaggcgc ttattccact
     1321 ggcgaaagat attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga
     1381 tatcgcaccg cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca
     1441 gcaggggatt ggtgcctggc cggatgcgca gcgggttaac ttttaccttg ccgggcgcgc
     1501 gccgcacaca ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga
     1561 cgttaaacct gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca
     1621 tttccgcccg acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc
     1681 attgctggag aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc
     1741 ggcagttttc tcgatacctt catctagggt gatgatcggc tgataaccta actcttcctg
     1801 cgcacgcgta atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag
     1861 cggcggctct tttgctgact tgcggcctaa acgctccatg ctgcgggcga tcatatccag
     1921 catcgggtag gggacggaac gaatacgaca gtcaatattc aactcgtcga tcagcttctg
     1981 cacgatgctg cgcagtgtgc gatgctcgcc gttggtgata ttgtacacac gcccggaagg
     2041 tagcttatcg caggcttcct ggcttgccag ccacattgcg tgcacggtat tttcatagta
     2101 ggtcatatcg accagcgcac tgccgccatg cggtaacaga atactgccgt agtggtgcat
     2161 catatgcgcc agacggggaa taaagacttt atcgtgcggt ccgaacagac tttgtgggcg
     2221 cagaatagta aagcgcgttt gtggattcgc ctgcgaaagc atattgatca cttcttcgct
     2281 ggctgctttg ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat cttctttaat
     2341 atcgcgatgg tggtgataat cgaagtacag ggagggggaa gagatatgaa taaagttacg
     2401 cacaccccag gcgacagccc attcacccag gcggcgagtg gcgcgaacgt tagccagatc
     2461 gaaagcctgt tgtgtccccc agggtgaggt aaagctggag cagtgccaca gcgtatcaat
     2521 gcccgcgagc atcactttag cttgtgatga aaccagctcg gtcagatccg ccggaacaaa
     2581 ctctgcgccc attttttcca gcaatttgcc cattgcctcg ttgcgaccgg tcgctcgcac
     2641 gctgatgcct ttctggcata aaaactctac cgcgtttcga cctaagccgc tggtggcgcc
     2701 ggtaaccagt accttcatat caatccactg ttgttgagaa aatgacgtgc gcattcttcc
     2761 gtgatttccc ccatgatgca atgggaaaca tgaaagaata acgcaggttt tgtcgattaa
     2821 tctgtgcttt gttctgccag tctggcgatt tgttttgcca ttccgcgaaa aataaacaga
     2881 tgcgcgggga tcatcaataa ccagtaaaac agccccggca taccgtgcgg atgccagaaa
     2941 gcgcggacat cgatagtacg atagtcgcct ttatcttcca ggctaaaaca cagtcgtccc
     3001 agccccgacg ctttcatgcc aaataacaac gtaagttgtt tttccggttc aacgacaatc
     3061 actttccagc tatccaccgc atcgccagtc tgtaaatatt cgcgctccgg gcggcctttc
     3121 gccagcttat gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa aatattgcca
     3181 aagaaataac gctctttacc gccgatttgg ttcactacct gccataaagc agcaaggctg
     3241 gcggacgttt taacggtaaa ccccgcctgt ttggcaaaat aaccgtactc cggtcgccag
     3301 cgggcaaagg cctgagcgtc gtagccccag tcgctggagt tgaccagttt ttcctcctct
     3361 ttcaacgtgc tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat gagtgcacgt
     3421 agcgcggtat catccgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg
     3481 gtgggcggta cggaagtaat cacattgaga aaccacaccg aaatccagcg ggtggggagg
     3541 gggatgggga tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc
     3601 tgataactga gcacctctgg tccggcggct tcgaagatgc ggtgttcgct ggccggatga
     3661 tctaacaacg ccaccagata gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa
     3721 cgtacccagc gtggcggcgt taacactggc aggttgtaga ccatatcgcg catgacttcg
     3781 aacgccgctg aacctgcgcc aacgataatt ccggcccgaa gttcggtcac aggtacattc
     3841 gcttcacgaa gaatgtccgc cgtagcctga cgagcacgca gatgatccga ctgctcatgt
     3901 ggcggggcct gcaacgaact gagaaagatt aattgcttaa ctggtacttc acgtagcgca
     3961 tcgcggacgt tgagagccac ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg
     4021 ctgtgcacca gaaaatagac ggtatcgata tcctgcaaca gggccggaag gttatccggc
     4081 cagctgagat cgactttatg gcaactgacg tttgccagtt gcagctttgc aagcctgtcg
     4141 acatgacgtg ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg caccagatgc
     4201 tgaccaatgt agccactggc accgagaact aaaatgcgtt gcggcacgtc tctctcctta
     4261 acgcgccagg aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc gcgagacgtc
     4321 aagatgcgtc accaggcgga caatcggcga ggcgttaatc agcacgtttc tcgctttcat
     4381 gtattcgcct aacgcggcag cattttcttc cccgacgcga acaaacagca tattagtgtc
     4441 ctgacgcatc acatccgcgc ctgcttcacg cagttgctcc gccatccagg cggcgttgtc
     4501 gtgatcttcc tgcaaccgtg cgacgttatt tttcagggca tacatcccgg cggcagccag
     4561 aatcccggac tgacgcatcc cgccaccggc cattttccgc cagcggatgg cacgtttaat
     4621 gtaatcacga ttaccgacga gtaatgaacc gactggcgtc ccaagacctt tcgacaggca
     4681 aatggtgaac gaatcacaat attgcgtgat ctctttcagt tcgcagccgt aagccaccac
     4741 ggcattaaag atgcgcgcac cgtcaacatg cagcgccaga ttgcgcttgc gggtaaattc
     4801 ccatgcttct ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt tttctagact
     4861 gagtaatttg gtgcgggcga aatggatatc gtcgggtttg attttcatcg ccactttatc
     4921 cagcggtagc gtgccgtcgg cagccgcgtc gatgggttgc ggttgaatac tgcccagcac
     4981 cgctgcgcca ccggcttcaa acagataatt atgcgcggcc tgaccgacaa tatactcttc
     5041 gccgcgttcg cagtgactga gcagagcgac caggttagcc tgggtgccgg tcggcagaaa
     5101 aatggcggct tctttaccgg aaagctctgc ggcgtagtcc tgcagagcat taacggtagg
     5161 gtcgtctccg taaacgtcgt ccccaaccgg ggcggccatc attgcttcga gcatggcgcg
     5221 gctcggtcgg gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt attatgacgg
     5281 gaaatgccac cctttttacc ttagccagtt cgttttcgcc agttcgatca cttcatcgcc
     5341 gcgtccgctg atgattgcgc gcaacatata caagctaaat cctttggcct gttcgagttt
     5401 gatctgcggt ggaatggcta actcttcttt ggcgaccacc acatccacca gcaccggacc
     5461 gtcgatggag aaggcgcgtt gcagggcttc atcaacttca gacgcttttt ctacacggat
     5521 acccgtaatg ccgcacgctt cggcaatgcg ggcaaagttt gtgtcgtgta gttcggtgcc
     5581 gtcagtcaaa tagccaccag ctttcatctc catcgccaca aagcccagca cgctgttgtt
     5641 aaagacgaca attttcactg gcagtttcat ctgcactact gagaggaaat cgcccatcaa
     5701 catgctaaaa ccgccatcgc cgcacatggc gaccacctga cgttctggct ctgtcgcctg
     5761 cgcacccagc gcctgcggca tggcgttagc catcgaaccg tggttaaacg aacctaacag
     5821 gcgacgcttg ccgttcattt ttagataacg tgccgcccac accgttggcg taccaacgtc
     5881 acaggtgaaa atagcgtcat cggcggcaaa atgactaatt tgctgcgcca gatattgcgg
     5941 gtgaatggct ttctcgctcg gtttagctaa atcgtccagc cctttgcggg cgtcgcggta
     6001 atcttccagc gctttatcca gaaacttgcg atcggctttt tcttccacca atggaagcaa
     6061 tgcacgcaga gtcgacttga tatcgccgac cagtgccata tccaccttgc tgtgagcgcc
     6121 gatgctggct gggttgatat caatctgaat gattttggca tcggtcgggt agaaggcgcg
     6181 gtagggaaat tgcgtgccga gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc
     6241 tgacgagaag ccgattaacc cggtcattcc aacatcatac ggattatcgt attcgacatg
     6301 ttctttaccg cgcagggcat gaacaatagg cgctttaatt ttcccggcaa actcaactaa
     6361 ctctttatgc gcccccgcgc agccgctgcc acacatcagg gcgatattgc tggaataacg
     6421 cagcagttgc gccagtttgc gtaactcttc ttcttccggc gtcacgactg gttgtggcgc
     6481 atgataccag tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt cgcctggtaa
     6541 cacgacaacc gaaacgccac ggttaagcac cgctttgcgc atggcaatcg ccagtacttg
     6601 tgggatctgc tccgggctgg aaaccagctc gcaatagtga ctacattcgc ggaatagctc
     6661 ttgtgggtgg gtttcctgga aatagccgct gccaatttcg ctggagggaa tatgagcggc
     6721 aatcgccagt accggaacgt gattgcggtg gcaatcgaac aggccgttga ttaagtgcag
     6781 gttgccgggg ccgcacgatc cggcgcagac cgccagttct ccgctaagtt gtgcttcagc
     6841 gccagcggca aaggccgcca cttcttcgtg gcgggtggac atccactcga tggtgcccat
     6901 gcgattaaga ctgtcactaa gaccgttcag agagtcgcct gtgactcccc agatgcgttt
     6961 cacccctgcc gattcgagtg ttttggcgat ataagctgca accgtttgtt tcatggttct
     7021 ccatctcctg aatgtgataa cggtaacaag tttagttcat ctgacggagg gggaagggat
     7081 gggagagaaa ggaggcacta acggttaaat agcccgatga aaggaatatc atcgggcata
     7141 aggcgattat gcgagaacca aatccccctg cggatggcag gagcaggcca gtacgtaacc
     7201 ttcagcgatt tcggcgtcgg tcagcgtcat tgtactgctc accgtatatt caccggaaac
     7261 cacttttgtc ttacagcagc cgcaaacacc cgcacggcag gcagcgacaa ccggaacgtt
     7321 attgctttcc agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg
     7381 ttgcagtttg gtgaatttca gaccgctggt cgccgcttct gctactggag tgaagaattt
     7441 ctctttaaag aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc aatccatata
     7501 cggagccggg ccgcaggtca tcacggtacg tgaagctaag tcaggtacac ctgccagcag
     7561 ttcgcgagtg agacgaccag cgataaagcc ttcggtaacg ttattttctg ccaccagcgt
     7621 taccggatag ttacgccact catcggcgaa aataacatcc tgcggcgtac gcacgttgta
     7681 gatcacccgc acatcggcct gtggacggtt cttcgcaagc cagcgacgca tcgacataat
     7741 cggcgtgacg ccgcagcctg ccgccagcaa caggaattta tcttctgctt tatcgtcgca
     7801 ggtaaattcc cccatcgcgt ccgaaagcca gagataatca ccgcgtttta catcgcgcgt
     7861 cagccactgg gagccgacac cgtcatcaat ccgccgcacg gtcagggtga tatattcact
     7921 cacgcctggc gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct
     7981 gaccagtgca tattgcccgg cgcgatatgg gtagtaatcg tggcaaatca gggaaatcgt
     8041 ccacacatcc ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg ggcattgatt
     8101 cgttggcatc gtcatcgaca aactccttac gcgctcaaca gttgcttcat gtcttcttca
     8161 acagtggtga tagaacgcag gccgaatttc tcgttcagca ccgccagcag gtctggtgtc
     8221 aggaaaccag gtgcagtcgg gccggtgacg atatttttca cgcccagaga aagcagcgtc
     8281 agcagaatga cgatcgcttt ctgttcaaac caggagagca ccagcgacag cggcagatcg
     8341 ttcacaccac agcccagttt ctctgccaga gtgacagcca gaataatcgc tgagtaagca
     8401 tcgttacatt gacctgcatc taccagacgc ggcagacctt cgatatcgcc aaactcaagt
     8461 ttgttaaagc gatatttacc acaggcgagg gtcaggatca ggcagtcatc cggcacgctg
     8521 gtggcgaaat cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg
     8581 aagatatgac gcagtttttc acggctcacc agatcaatca gcgtatcagc agcgccaagc
     8641 agcgtctggc gaccaaaacc cacggtgata aggtgcggaa tttcgctgta cgggaagcct
     8701 gccatctgtt gcgcctgggt gataaccgca gagaaatcat caccgtccag atgacgcacg
     8761 ccaggccagc caacaatgct gcgggtccag atacgatcgt cataagcgcc tacggttggg
     8821 tcgatgatgc agttcgaggt catcacgatg gggccaggga aacgagcgaa ctccacttgc
     8881 tgattctgcc agccgctgcc gtagttaccg accagatgct tgaatttacg cagctccggg
     8941 tagccatgcg caggcagcat ttcgccgtgg gtgtagacat taacgcccgt gccttcggtc
     9001 tgttccagca ggttgtagag atctttgaga tcgtgaccgg aaatcagaat gcatttaccc
     9061 gccgtcgctt tgacgttgac ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct
     9121 gcatccagaa tgctcatcac tttgaagttc atctggccga tttccattga acactcaaga
     9181 agcgcgttca tatcggcagg ccaggtcccc agccacgcca tgattttatg gtactgggca
     9241 taaatatcgt tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc cgccgcacct
     9301 ttcaggccat acaggcacag cagacgcagg ccgagaatgt tttcgccaat cgccgcttta
     9361 tctttgttag gggtaaattc tgctgcctga cgttgcagct cgccgagatc atcgctcacc
     9421 agttgcaggt cagccatcgg gttatcgacg cgcgcgttgg catctacagc caggcattgt
     9481 gctttcagcg cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat acgcggagaa
     9541 tcgaagttaa cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg
     9601 tggttgatga tgccgtattc acacgctttt accgcccagg cagaaagccc ttgcagcgcc
     9661 gcgatgagta aatcctgaag gtcagaagtt tccgccgttt taccacacat cccctgcgcg
     9721 tatgagcagc cgtttcctgc cggagtacgg atagtttgtt cacattgcac acaaaacatg
     9781 atcacacctt ttaaagttat atttaatata catgtttaag gttaagacgc ttaacgcggg
     9841 gataaaaggg atttttcatg caactttaag ggagattgat ttagcgcaat tttggcggca
     9901 gggatctacc gccagagagg tattacgcag agaaaaaggc gatgaggatc ggcactaaca
     9961 ggctaagaat aaaaccgtga acaattgccg ccgggaccat atccagcccg ccagtacgtt
    10021 gaagaacggg cagggtgaaa tccattgatg tggcaccgca taagcccagt gcagtagagc
    10081 ggctgcggcg aatcagccca gggatcaaca taatagcaat cagttcacgg gccagatcat
    10141 taaaaaacgc cgcgctcccg attaccggac caaaagattc ggtcaataaa ataccggaaa
    10201 gagaatacca gccgaaaccg gaggccattg ccagcgcggt attgatgggg agatcaagaa
    10261 taaaggcgtt aattaaacca ccaattaatg aactgacaac caccaccacg gcgacaatca
    10321 ttccccggcg attaaggaca atctgcttta aggtcatgcc attattgcgc aactgaatac
    10381 caacgaggaa aagtagcaaa attaacgtgt attcactggc ttcggtcgcg tgttgtaaga
    10441 aagccagtcc actcagacca atggcaaaac caatcactac tacgccgcac agttttagcg
    10501 actccagcgc catcgcaata cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg
    10561 gcaggcctcg ctccagccac atcagggcgg caatattaca cagtaaaata acggtaatac
    10621 tgacggcaga ataatgcaga atcgccaaca ggttactggc gaggttatcg agaaacgcca
    10681 gactgatacc cataaaaaag agaataaggt aaaccatcca gcttaatagc tgattaataa
    10741 cttttaacgc agcttgttgg cgaagcggaa tgaggtaacc tacaatcagg ggaaccagaa
    10801 tgattaacag cccagaaaac atgaaaaccc agtccttgca aagatgaagt cgaaatgcgc
    10861 gatgacacac tactgaaagc ggaaggacga gtaaagttgc aattaaaagg aaatgttatg
    10921 cataaggagc agtagagtat tcgttttcat ttaaagatat tcttgcgctt taattacaaa
    10981 ctgcaccgat gttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc ggggcagccc
    11041 acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc gacccggtag
    11101 ccgtaaggga taagtcgagg gcaccgcgca gcggcgattt tgttcgccag agcccggggg
    11161 ggcagggggc ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt ggcaatatgt
    11221 ggcttagctc atgaaaggag cgcaacaaga tgatgaatca acatataaca acatcttaaa
    11281 aaaaggcctg acattacgcc aggccttctg cgttaattaa tcacgctttt ccagcagggt
    11341 ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga agaaccacag
    11401 ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg ccgggttaac
    11461 agaagtgtta gtcaccggaa tactaattaa gtgaatcagg gttaaggcca gaccaatagc
    11521 gatcggcgca aaacctgccg gcgcgaattt gtcggttgcg ccgtggatca ccaacaggaa
    11581 acctgcactc aataccagtt caactaccag cg
//
LOCUS       DAZOZD010000127        11517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOZD010000127 DAZOZD010000000
VERSION     DAZOZD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.127"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            126..797
                     /locus_tag="ACHUPB_002774"
     CDS             126..797
                     /locus_tag="ACHUPB_002774"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR0759541.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            807..2003
                     /locus_tag="ACHUPB_002775"
                     /pseudo
     CDS             807..2003
                     /locus_tag="ACHUPB_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2003..2563
                     /locus_tag="ACHUPB_002776"
     CDS             2003..2563
                     /locus_tag="ACHUPB_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0759542.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2565..3338
                     /locus_tag="ACHUPB_002777"
     CDS             2565..3338
                     /locus_tag="ACHUPB_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR0759543.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3526..3801
                     /gene="frmR"
                     /locus_tag="ACHUPB_002778"
     CDS             3526..3801
                     /gene="frmR"
                     /locus_tag="ACHUPB_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR0759544.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3836..4945
                     /gene="frmA"
                     /locus_tag="ACHUPB_002779"
     CDS             3836..4945
                     /gene="frmA"
                     /locus_tag="ACHUPB_002779"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR0759545.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5038..5871
                     /gene="fghA"
                     /locus_tag="ACHUPB_002780"
     CDS             5038..5871
                     /gene="fghA"
                     /locus_tag="ACHUPB_002780"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0759546.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6097..6636)
                     /locus_tag="ACHUPB_002781"
     CDS             complement(6097..6636)
                     /locus_tag="ACHUPB_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR0759547.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6738..7949)
                     /gene="mhpT"
                     /locus_tag="ACHUPB_002782"
     CDS             complement(6738..7949)
                     /gene="mhpT"
                     /locus_tag="ACHUPB_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR0759548.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8236..9249)
                     /gene="mhpE"
                     /locus_tag="ACHUPB_002783"
     CDS             complement(8236..9249)
                     /gene="mhpE"
                     /locus_tag="ACHUPB_002783"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR0759549.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9246..10196)
                     /gene="mhpF"
                     /locus_tag="ACHUPB_002784"
     CDS             complement(9246..10196)
                     /gene="mhpF"
                     /locus_tag="ACHUPB_002784"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR0759550.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10193..11002)
                     /gene="mhpD"
                     /locus_tag="ACHUPB_002785"
     CDS             complement(10193..11002)
                     /gene="mhpD"
                     /locus_tag="ACHUPB_002785"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR0759551.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11012..>11517)
                     /gene="mhpC"
                     /locus_tag="ACHUPB_002786"
     CDS             complement(11012..>11517)
                     /gene="mhpC"
                     /locus_tag="ACHUPB_002786"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0759552.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2765 a         3108 c         2879 g         2765 t
ORIGIN      
        1 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
       61 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      121 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      181 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      241 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      301 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      361 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      421 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      481 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      541 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      601 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
      661 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
      721 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
      781 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
      841 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
      901 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
      961 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1021 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1081 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1141 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1201 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1261 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1321 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1381 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1441 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1501 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1561 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1621 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     1681 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     1741 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     1801 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     1861 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     1921 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     1981 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2041 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2101 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2161 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2221 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2281 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2341 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2401 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2461 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2521 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2581 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     2641 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     2701 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     2761 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     2821 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     2881 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     2941 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3001 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3061 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3121 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3181 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3241 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3301 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3361 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3421 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3481 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3541 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3601 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     3661 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     3721 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     3781 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     3841 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     3901 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     3961 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4021 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4081 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4141 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4201 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4261 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4321 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4381 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4441 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4501 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4561 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4621 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     4681 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     4741 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     4801 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     4861 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     4921 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     4981 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5041 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5101 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5161 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5221 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5281 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5341 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5401 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5461 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5521 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5581 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     5641 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     5701 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     5761 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     5821 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     5881 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     5941 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6001 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6061 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6121 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6181 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6241 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6301 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6361 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6421 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6481 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6541 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6601 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     6661 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     6721 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     6781 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     6841 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     6901 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     6961 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7021 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7081 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7141 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7201 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7261 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7321 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7381 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7441 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7501 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7561 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7621 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     7681 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     7741 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     7801 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     7861 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     7921 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     7981 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8041 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8101 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8161 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8221 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8281 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8341 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8401 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8461 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8521 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8581 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     8641 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     8701 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     8761 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     8821 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     8881 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     8941 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9001 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9061 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9121 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9181 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9241 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9301 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9361 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9421 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9481 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9541 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9601 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
     9661 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
     9721 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
     9781 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
     9841 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
     9901 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
     9961 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10021 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10081 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10141 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10201 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10261 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10321 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10381 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10441 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10501 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10561 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10621 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    10681 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    10741 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    10801 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    10861 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    10921 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    10981 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11041 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11101 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11161 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11221 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11281 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11341 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11401 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11461 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAZOZD010000128        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOZD010000128 DAZOZD010000000
VERSION     DAZOZD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.128"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUPB_002787"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUPB_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0759553.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUPB_002788"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUPB_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0759554.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUPB_002789"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUPB_002789"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0759555.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUPB_002790"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUPB_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0759556.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUPB_002791"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUPB_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0759557.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUPB_002792"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUPB_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0759558.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUPB_002793"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUPB_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0759559.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUPB_002794"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUPB_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0759560.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUPB_002795"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUPB_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0759561.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUPB_002796"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUPB_002796"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0759562.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUPB_002797"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUPB_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0759563.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUPB_002798"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUPB_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0759564.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUPB_002799"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUPB_002799"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR0759565.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZOZD010000129        11355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOZD010000129 DAZOZD010000000
VERSION     DAZOZD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.129"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUPB_002800"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHUPB_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0759566.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUPB_002801"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="ACHUPB_002801"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0759567.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="ACHUPB_002802"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="ACHUPB_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0759568.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUPB_002803"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHUPB_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0759569.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUPB_002804"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="ACHUPB_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0759570.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUPB_002805"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="ACHUPB_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0759571.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUPB_002806"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="ACHUPB_002806"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0759572.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUPB_002807"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="ACHUPB_002807"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0759573.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUPB_002808"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHUPB_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0759574.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUPB_002809"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="ACHUPB_002809"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0759575.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUPB_002810"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="ACHUPB_002810"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0759576.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUPB_002811"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="ACHUPB_002811"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0759577.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="ACHUPB_002812"
     CDS             10898..>11355
                     /locus_tag="ACHUPB_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0759578.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAZOZD010000130        11309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOZD010000130 DAZOZD010000000
VERSION     DAZOZD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.130"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHUPB_002813"
     CDS             53..352
                     /locus_tag="ACHUPB_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0759579.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHUPB_002814"
     CDS             complement(472..651)
                     /locus_tag="ACHUPB_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0759580.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHUPB_002815"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHUPB_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0759581.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHUPB_002816"
     CDS             2090..2668
                     /locus_tag="ACHUPB_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0759582.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUPB_002817"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUPB_002817"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0759583.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHUPB_002818"
     CDS             4347..5945
                     /locus_tag="ACHUPB_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0759584.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUPB_002819"
     CDS             complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHUPB_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0759585.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUPB_002820"
     CDS             complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHUPB_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0759586.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7962..8933
                     /gene="manX"
                     /locus_tag="ACHUPB_002821"
     CDS             7962..8933
                     /gene="manX"
                     /locus_tag="ACHUPB_002821"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0759587.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8996..9796
                     /gene="manY"
                     /locus_tag="ACHUPB_002822"
     CDS             8996..9796
                     /gene="manY"
                     /locus_tag="ACHUPB_002822"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0759588.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUPB_002823"
     CDS             9809..10660
                     /gene="manZ"
                     /locus_tag="ACHUPB_002823"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0759589.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10715..11173
                     /locus_tag="ACHUPB_002824"
     CDS             10715..11173
                     /locus_tag="ACHUPB_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0759590.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2786 a         2838 c         2838 g         2847 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggg ttatcggcat cgtgctgaac attggcctga
     6721 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
     6781 gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
     6841 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
     6901 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
     6961 ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
     7021 gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac
     7081 gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct
     7141 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
     7201 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
     7261 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
     7321 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
     7381 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
     7441 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
     7501 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
     7561 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
     7621 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
     7681 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
     7741 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
     7801 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
     7861 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
     7921 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
     7981 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
     8041 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
     8101 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
     8161 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
     8221 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
     8281 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
     8341 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
     8401 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
     8461 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
     8521 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
     8581 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
     8641 ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta tttaccaacc
     8701 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
     8761 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
     8821 atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc cgtaaggttt
     8881 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
     8941 tgttgattat cactcagttt tcacacttaa gtcttacgta aacaggagaa gtacaatgga
     9001 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
     9061 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
     9121 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
     9181 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcta tcatttctac
     9241 cattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg caatccctct
     9301 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
     9361 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
     9421 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
     9481 tggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
     9541 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
     9601 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
     9661 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
     9721 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
     9781 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
     9841 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
     9901 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
     9961 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    10021 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    10081 acagcgtgct aatggcgcag agatcgacga cggtgctatc aacggtatca aagtcggttt
    10141 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    10201 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    10261 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    10321 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    10381 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    10441 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    10501 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    10561 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    10621 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    10681 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    10741 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    10801 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    10861 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    10921 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    10981 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    11041 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    11101 tcggctgtta atccgcgttc gaaatatcga cgatctggaa aaaatttata agcttctcgt
    11161 ttcaactcaa taagttatga atttagccaa agctatgttt agtgtatttt taataatcag
    11221 acatagctta ggctatatta cctcttccct tatttgttat ttattttaac gtttcattga
    11281 tatataaatc caaatgaaaa tcgttatca
//
LOCUS       DAZOZD010000131        11307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOZD010000131 DAZOZD010000000
VERSION     DAZOZD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.131"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUPB_002825"
     CDS             <1..208
                     /locus_tag="ACHUPB_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0759591.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="ACHUPB_002826"
     CDS             300..2984
                     /locus_tag="ACHUPB_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0759592.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="ACHUPB_002827"
     CDS             2981..4069
                     /locus_tag="ACHUPB_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0759593.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHUPB_002828"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHUPB_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHUPB_002829"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHUPB_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHUPB_002830"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHUPB_002830"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0759594.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="ACHUPB_002831"
     CDS             8928..9479
                     /locus_tag="ACHUPB_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0759595.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHUPB_002832"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHUPB_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0759596.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2968 a         2772 c         2935 g         2632 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
    11041 ggggcggcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
    11101 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
    11161 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
    11221 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccgattaca
    11281 taattaatag ctcacctttg tctggaa
//
LOCUS       DAZOZD010000132        11276 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOZD010000132 DAZOZD010000000
VERSION     DAZOZD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11276
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.132"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            73..207
                     /locus_tag="ACHUPB_002833"
     CDS             73..207
                     /locus_tag="ACHUPB_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0759597.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            186..290
                     /gene="speFL"
                     /locus_tag="ACHUPB_002834"
     CDS             186..290
                     /gene="speFL"
                     /locus_tag="ACHUPB_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0759598.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            601..2799
                     /gene="speF"
                     /locus_tag="ACHUPB_002835"
     CDS             601..2799
                     /gene="speF"
                     /locus_tag="ACHUPB_002835"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR0759599.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2796..4115
                     /gene="potE"
                     /locus_tag="ACHUPB_002836"
     CDS             2796..4115
                     /gene="potE"
                     /locus_tag="ACHUPB_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0759600.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4171..5811)
                     /gene="pgm"
                     /locus_tag="ACHUPB_002837"
     CDS             complement(4171..5811)
                     /gene="pgm"
                     /locus_tag="ACHUPB_002837"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0759601.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5837..6382)
                     /gene="seqA"
                     /locus_tag="ACHUPB_002838"
     CDS             complement(5837..6382)
                     /gene="seqA"
                     /locus_tag="ACHUPB_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0759602.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6567..7331
                     /gene="ybfF"
                     /locus_tag="ACHUPB_002839"
     CDS             6567..7331
                     /gene="ybfF"
                     /locus_tag="ACHUPB_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0759603.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7471..7764
                     /gene="ybfE"
                     /locus_tag="ACHUPB_002840"
     CDS             7471..7764
                     /gene="ybfE"
                     /locus_tag="ACHUPB_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0759604.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7904..8434
                     /gene="fldA"
                     /locus_tag="ACHUPB_002841"
     CDS             7904..8434
                     /gene="fldA"
                     /locus_tag="ACHUPB_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0759605.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8644..8730
                     /gene="uof"
                     /locus_tag="ACHUPB_002842"
     CDS             8644..8730
                     /gene="uof"
                     /locus_tag="ACHUPB_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0759606.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8723..9169
                     /gene="fur"
                     /locus_tag="ACHUPB_002843"
     CDS             8723..9169
                     /gene="fur"
                     /locus_tag="ACHUPB_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0759607.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9254..9580)
                     /gene="chiQ"
                     /locus_tag="ACHUPB_002844"
     CDS             complement(9254..9580)
                     /gene="chiQ"
                     /locus_tag="ACHUPB_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0759608.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9630..11036)
                     /gene="chiP"
                     /locus_tag="ACHUPB_002845"
                     /pseudo
     CDS             complement(9630..11036)
                     /gene="chiP"
                     /locus_tag="ACHUPB_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2756 a         2775 c         2807 g         2938 t
ORIGIN      
        1 cgcaaatata aaacacgaaa ttacaacatt taagtaacaa aacagcatct aatatggcga
       61 tattcataat taatgattta ttatctgggg tatgatcgtt ttttgttgat cttcttcaca
      121 gattatagcc atttcatgga tagaataact ctaccttcaa ctgacacagc aagaggtaaa
      181 ggtaaatgga aaataataac cgcttaatgc ctcatataag gcggacaacc catatcatga
      241 agtttgccca tcgtaatagt tttgactttc atttctttaa tgcccgctag tcttctgact
      301 aaagggcacc ccaacgtaca ggtctccctg actttaagca ttacaggtta atacctgtat
      361 tcctcggtgc tcatatactg ctaacccttt taaactctaa ataattcgag tcgcagcact
      421 tgcaacttga ggtatgacga gtatagccag ttaccgggct ggtctgggtt attgcatctg
      481 caaaaagcaa actactgatt tatttatcag cggtggagct ttgctttttt tccggcgtga
      541 tcgatttctc ctttgagaaa ttgaggacct gctattacct gaaataaaga gatgaataaa
      601 atgtcagaat taaaaattgc cgttagccgt tcttgcccgg attgtttttc cactcatcgt
      661 gcatgcgtga atattgacga aagtaattat attgacgttg ccgccattat tttatcagtc
      721 aatgatgttg aacgtggaaa actcgatgaa atagacgcta ctggctatgg cattcctgtt
      781 tttattgcaa cggaaaatga agaacgtgta ccagcagaat atctgccacg tatttctggt
      841 gtcttcgagc attgcgaatc acggaaagag ttttatggac gtcagttaga aaccgctgct
      901 agccattatg aaacgcaact gcgcccgccc ttcttccgcg cactggtcga ttatgtcaat
      961 cagggcaata gcgcttttga ctgcccgggt catcagggag gtgagttttt ccgtcgtcat
     1021 ccggcaggga atcagttcgt ggagtacttc ggagaaacgc tgttccgttc cgacttatgc
     1081 aacgcagacg tagcaatggg cgatctgctg attcacgaag gcgcgccatg cattgcacag
     1141 caacatgcgg caaaagtgtt taatgccgat aaaacatact tcgttctaaa tggcacttca
     1201 tcttctaaca aagtggtttt aaacgccctg ctaacaccgg gtgatctggt gctgtttgat
     1261 cgcaataacc acaaatctaa ccaccacgga gcgttgctac aggctggtgc aacaccggtt
     1321 tatctggaaa cggcacgtaa cccgtatggc tttatcggtg gcattgatgc gcactgtttt
     1381 gaagaaagtt acctgcgtga gctgatcgcg gaagtcgcac cgcagcgggc aaaagaggct
     1441 cgtcctttcc gcctcgctgt gattcagtta ggcacctacg acggtacgat ttataacgcc
     1501 cgccaggtgg tggataaaat tggtcatctg tgtgactaca ttctgtttga ctccgcatgg
     1561 gttggctatg agcaatttat cccgatgatg gctgactgtt cgccgctgtt gctggatctt
     1621 aatgagaacg atccgggtat tctggttacg caatccgtac ataaacagca ggctggtttt
     1681 tctcaaactt cacaaatcca taaaaaagac agccacatca aagggcaaca gcgttatgta
     1741 ccgcacaaac gcatgaacaa cgcctttatg atgcacgcct ccaccagccc gttctatccg
     1801 ctgtttgccg cactggatat caacgccaaa atgcatgaag gtgtcagcgg tcgtaatatg
     1861 tggatggatt gtgtggtaaa tggcattaat gcccgcaaac tgatcctcga taactgtcag
     1921 catattcgtc cgttcgtacc tgaactggtg gatggtaaac cctggcagtc gtatgaaaca
     1981 gcgcaaattg cggttgatct gcgcttcttc cagtttgtac caggggaaca ctggcattct
     2041 tttgaaggct atgcagagaa tcaatacttt gtcgatcctt gcaaactgtt gctgacaacc
     2101 ccaggtattg atgcacgtaa cggcgaatat gaagcgttcg gtgtacccgc gacgattctt
     2161 gctaacttcc tgcgcgaaaa tggcgtagtg ccggaaaaat gcgatcttaa ctccatcctc
     2221 ttcctgctga ctccggcaga agatatggcc aaacttcagc aacttgttgc cctgctggta
     2281 cgcttcgaaa aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac
     2341 aaacagcatg aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat
     2401 gatttgtatg cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac
     2461 ttcccacacg tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg
     2521 gagctggttc gtctgccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat
     2581 cctccgggtg tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac
     2641 ttcagcgctc tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt
     2701 gtctatatcg aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt
     2761 gatgcgcaaa gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa
     2821 tgggcgtcgt tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca
     2881 tgctgccgac aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag
     2941 ccgtaggctc aatggcactg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat
     3001 caggtggtat gggcggttat gccgagtatg ctttcggtaa atccggtaac tttatggcga
     3061 actataccta cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg
     3121 gttacggcac cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca
     3181 tcggggtgct gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa
     3241 tcagtagcat taccgtgtgg ggggtcatta ttccggtggt aggcctgtgc attatcggct
     3301 ggttctggtt tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct
     3361 tcagtgcagt aggttcttcc atcgctatga cgctgtgggc ttttctcggt ctggagtctg
     3421 cgtgtgcgaa tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg
     3481 gcggtacgtt aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg
     3541 tgcctaatat ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt
     3601 tcacaccaga agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc
     3661 tgcttggctg gcagttcacc attgcccagg tgtttaagtc ttcatctgat gaaggctact
     3721 tccctaaaat tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca
     3781 ttgtgattat ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt
     3841 tcaacgtgct ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg
     3901 cggcattagt cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa
     3961 actttgttgc ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag
     4021 aagccatgct gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat
     4081 caccacgctt tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc
     4141 ttgcgaccgc ccttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat
     4201 ctcaaccgct tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta
     4261 gatcttatat gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat
     4321 cactttcaga ccgccgatag aagcaccatt gcccggtgcc gccgtcaggc gcgcggtgat
     4381 cgggtcgcct gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc
     4441 tttttgtgcg gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt
     4501 tgccagttcg ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc
     4561 cagcagacac atgatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag
     4621 gaaggaagcc cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc
     4681 aacaaaccat ttgaaaccta ccgggacttc taccagcttg cggcccaagt cattgaccac
     4741 acggtcgatc atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg
     4801 cggacgatgc tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa
     4861 acctgccgga gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc
     4921 gaacttatca cgcaatgcca gcaggcccgc catcgcacac tcggaggagc agtccatacg
     4981 gatcgcgccg tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac
     5041 gatggtcagg ttgaggttgt aatactcgcc aattcgcttc cagtattcga taccggaacc
     5101 acccagcgga tcaacgccca gcgtcaggcc cgttttctga atcgccgcca tatcaacgat
     5161 atcggccaga ccttccacaa actgctgcac cagatcctgc tctttcacat gaccggatgc
     5221 catcgcttcg tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt
     5281 ggcacgatct tccaccactt tagtgacgtt ggtatcagcc gggccaccat ttggcggatt
     5341 gtacttgata ccaccatctt ccggcgggtt atgggagggt gtaatcacga taccgtctgc
     5401 cagcgggcca ccttttttat tatgaaccag gatggcattg gaaacggcag gcgtcggagt
     5461 gaagccattg ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac
     5521 ggaaatgaac gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt
     5581 gatgccgttt ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc
     5641 gttaaagctg tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg
     5701 ctccgcattc cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt
     5761 aatcaaatca ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc
     5821 ctttgtctgc aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt
     5881 gcatcgactg catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga
     5941 tcacccaata cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca
     6001 gcgtttgttc atctgccgca aagtaaacgc gtgtacgacc gtgcaacgat tccgttgctt
     6061 cggcaaacgc ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga
     6121 ccgctcgctt ttgctctgcg tattcgtcag aaagcagaag ttcacgcatt gcgcgaacct
     6181 tgtctttaat cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct
     6241 ctttcgtcac cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa
     6301 tgtcggatgc gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat
     6361 catcaacttc aatcgttttc atcttaatcc agtacagtgt cttagctgaa tacaagtcgc
     6421 caagagtata tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact
     6481 cggaataaac cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc
     6541 gacaacgcga aaaaaagaac tttgccatga aattgaatat ccgcgcgcaa actgcacaaa
     6601 accagcacaa taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc
     6661 ttggcgtact ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta
     6721 accacggtct ttcaccgaga gatccggtaa tgaattaccc ggcgatggcg caggatcttg
     6781 ttgatactct ggatgcacag cagatcgaca aagcaacatt tatcggtcac tccatgggcg
     6841 gtaaagcggt aatggcactt acggcactgg cccgcgatcg catcgataaa ctggtggcga
     6901 tcgatatcgc accggtcgac tatcacgtac gccgtcatga tgagattttc gcggctatca
     6961 acgcggtcag tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcaccagc
     7021 atcttaatga agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc
     7081 gctttaacgt gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc
     7141 cggcatggga tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc
     7201 agtaccgtga tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg
     7261 cgggtcactg ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc
     7321 tcaatgatta acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg
     7381 ttggcgtgac tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg
     7441 ctgattgttt cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca
     7501 ttagatctgt tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc
     7561 gatgaacagc tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt
     7621 aagcgtgtcg aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggag
     7681 tcgcgcaata tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg
     7741 ctgcgtagtc agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc
     7801 tcgtttgctg attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat
     7861 tttccacccc catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat
     7921 ctttttcggc agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct
     7981 tggtaaagac gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc
     8041 ttatgacatt ctgctgctgg gcatcccaac ctggtattat ggcgaagcgc agtgtgactg
     8101 ggatgacttc ttcccgactc tcgaagagat tgatttcaac ggcaaactgg ttgcgctgtt
     8161 tggttgtggt gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg
     8221 cgacatcatt gaaccgcgcg gtgcaacgat cgttggtcac tggccaactg cgggctatca
     8281 tttcgaagca tcaaaaggtc tggctgatga cgaccacttt gtcggtctgg ctatcgacga
     8341 agaccgtcag ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga
     8401 agagttgcat ctcgacgaaa ttctcaatgc ctgatgtgat gcggcgtaga ctcatgtcta
     8461 cgccgtatta atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt
     8521 tgtacctgta caatgtcccg gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc
     8581 acgtttttat taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct
     8641 ataatgatac gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc
     8701 ttagtaacag gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct
     8761 gaaagtaacg cttcctcgtt taaaaatcct agaagttctt caggagccgg acaaccatca
     8821 cgtcagtgcg gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc
     8881 tacggtatat cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt
     8941 tgaaggcggt aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatctg
     9001 cctcgactgc ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga
     9061 aattgccgca aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg
     9121 tgccgaaggc gattgccgcg aagatgaaca tgcgcacgaa ggcaaataag ccagcctgaa
     9181 tgagaaaaag ccaacctgcg ggttggcttt tttatgcaag ggaaataaga aaagcgagag
     9241 ttacagctct cacttatttg ttattactgc gaatttcctg ccaaacttta tcacaatcgg
     9301 ctttcaccgc ctgatcattt ccggtttgcg ttgcacgcag atactgctga taatccagta
     9361 cgcggacact ttcctggtcg gcaaaatgct gatgttgttt ctctttcttc agcacgttta
     9421 acacgctctg gcaggcttca attttttctg gcgaaccttg tgcggtgttg atacaggcgc
     9481 tgtatgcctc tttcagacga ctgtcttcct gtggcgcggt tgattgcgca caagctacca
     9541 gccccgatgc cattatggcg atgagaatca gttttttcat agcaaattcc tcaaacgtgc
     9601 ggcaggcaca aaacctgccg cgtcgggcat cagaagatgg tgaatggtgc gattaccata
     9661 aatttcacgt cacgctcatc ctggaagatg ttgccgtaac caccgcccca gcttgggatg
     9721 tcggagtggt tgtcgtattc ggtgaagtgc agtttgaaca tcgtgccttt ggcgcgaccg
     9781 tcctgaatgg tgtagaccgc atccaggctg tatgcagact cttcaatagt ccggtttttg
     9841 tcgtagtacg catccggatt gctctgctag gtcgcaggtt tagcatccca tgcgtaaacg
     9901 taggaagcgc cgatggcgaa gcctggaaga ttccagtttt tcaggtcata catcgcaccg
     9961 aagaagaccg ctttttcgcc gttagcgttg aagtcggaac ggttatccca ccagatatcc
    10021 aggcggccgt tggaggaagc gtaggttgga gtcatacgtt gcaggaagta tccctgctga
    10081 ccgtcagcct taacccaggt gccttcgagg cgcaaatcca ctacgtcagc cgcccggtaa
    10141 ccaaaggtca acgcttgcag ccaggcggtg ccgtcataaa ggtcgttgac gctgcgatcg
    10201 tcaactttat cgcgcgtacc atagaactgg tagctggtgg ttaacgggct accggcgata
    10261 tcaaatttgt agctggcttt ggcaaaatat tgatcgatat acccttccgc ctgaccaaat
    10321 gccgcttcca gtacgaagtt atttttgaag tcgtatttcg ccccaaggga gtgcagataa
    10381 tcaactttgg tggttttatc gttctgataa aactcatcca tttccagatg ccacggcgct
    10441 ttgtattcgt tggtccacat gtaggagaaa ctcaacgcac cagcatcgcc gtaatcaaaa
    10501 ttcgccccgg cttccgcccc ctgataagta cctggcataa agctccagtg cggcgctaac
    10561 agcgtttgac cagttggctg aatgtaacct gccctcgccc aaaccggacc gtatttaaat
    10621 ttggccgcag ctttatacag gcttataccg cttttgtcgc cggaccagtc ttcgtcatag
    10681 gctttattac tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc
    10741 atttcaatcg ccgtaaacgc agcaatatca aggccgaaca tatcagcagc ataaccggac
    10801 tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt tggttttgta tttgtcgccg
    10861 tcggtaacat ctttacggtc gcgttcacgc tgccagtaat atataccgcc ggttaaggtt
    10921 gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg ccataaagcc cgacattgct
    10981 gtaacaccgg cgatagccag cgccagcgta ctacgtttgc cactaaacgt acgcatgggt
    11041 taatcctctt tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa ataaaaatta
    11101 aaaaagtagc gttgagaaaa gtctcgctga ctacaggaac agactatttt tcgcgacgcg
    11161 aattatcaat ctctttctgt aaccagaatg taagaacatg agcaaccgca catattttga
    11221 tgatttttgt tacaaggctt aagtttgcgg ttgaaaggct tgcagaagaa taaaag
//
LOCUS       DAZOZD010000133        11262 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOZD010000133 DAZOZD010000000
VERSION     DAZOZD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.133"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1224
                     /gene="narU"
                     /locus_tag="ACHUPB_002846"
     CDS             <1..1224
                     /gene="narU"
                     /locus_tag="ACHUPB_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0759609.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1306..5046
                     /gene="narZ"
                     /locus_tag="ACHUPB_002847"
     CDS             1306..5046
                     /gene="narZ"
                     /locus_tag="ACHUPB_002847"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR0759610.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5043..6587
                     /gene="narH"
                     /locus_tag="ACHUPB_002848"
     CDS             5043..6587
                     /gene="narH"
                     /locus_tag="ACHUPB_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0759611.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6587..7282
                     /gene="narW"
                     /locus_tag="ACHUPB_002849"
     CDS             6587..7282
                     /gene="narW"
                     /locus_tag="ACHUPB_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0759612.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7279..7959
                     /gene="narI"
                     /locus_tag="ACHUPB_002850"
     CDS             7279..7959
                     /gene="narI"
                     /locus_tag="ACHUPB_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0759613.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8038..8931
                     /locus_tag="ACHUPB_002851"
     CDS             8038..8931
                     /locus_tag="ACHUPB_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR0759614.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="ACHUPB_002852"
     CDS             complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="ACHUPB_002852"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR0759615.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10045..10614
                     /locus_tag="ACHUPB_002853"
     CDS             10045..10614
                     /locus_tag="ACHUPB_002853"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR0759616.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="ACHUPB_002854"
     CDS             complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="ACHUPB_002854"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR0759617.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10945..>11262)
                     /locus_tag="ACHUPB_002855"
     CDS             complement(10945..>11262)
                     /locus_tag="ACHUPB_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0759618.1"
                     /translation="KKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVM
                     NEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMGRNYLASVLTGS
                     GLS"
BASE COUNT         2614 a         2938 c         3001 g         2709 t
ORIGIN      
        1 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
       61 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      121 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      181 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      241 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      301 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      361 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      421 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      481 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      541 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      601 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      661 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      721 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      781 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      841 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      901 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
      961 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1021 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1081 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1141 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1201 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1261 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1321 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1381 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1441 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1501 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1561 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1621 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1681 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1741 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1801 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1861 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1921 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     1981 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2041 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2101 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2161 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2221 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2281 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2341 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2401 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2461 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2521 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2581 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2641 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2701 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2761 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2821 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2881 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     2941 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3001 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3061 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3121 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3181 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3241 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3301 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3361 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3421 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3481 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3541 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3601 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3661 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3721 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3781 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3841 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3901 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     3961 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4021 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4081 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4141 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4201 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4261 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4321 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4381 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4441 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4501 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4561 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4621 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4681 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4741 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4801 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4861 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4921 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     4981 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5041 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5101 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5161 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5221 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5281 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5341 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5401 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5461 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5521 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5581 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5641 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5701 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5761 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5821 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5881 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     5941 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6001 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6061 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6121 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6181 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6241 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6301 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6361 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6421 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6481 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6541 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6601 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6661 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6721 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6781 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6841 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6901 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     6961 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7021 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7081 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7141 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7201 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7261 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7321 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7381 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7441 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7501 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7561 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7621 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7681 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7741 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7801 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7861 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7921 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     7981 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8041 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8101 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8161 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8221 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8281 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8341 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8401 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8461 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8521 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8581 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8641 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8701 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8761 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8821 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8881 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     8941 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9001 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9061 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9121 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9181 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9241 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9301 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9361 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9421 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9481 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9541 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9601 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9661 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9721 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9781 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9841 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9901 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
     9961 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10021 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10081 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10141 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10201 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10261 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10321 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10381 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10441 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10501 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10561 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10621 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10681 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10741 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10801 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10861 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10921 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    10981 gtagtttctg cccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    11041 atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt
    11101 tcctcttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc aatgcagctt
    11161 attctccacg tgccagtggt ttcggattgc tgtggcgaac ttctctgcgg ttaaatcagc
    11221 agaactgata taatatctga ccgtcatttc tggctctttc tt
//
LOCUS       DAZOZD010000134        11261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOZD010000134 DAZOZD010000000
VERSION     DAZOZD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.134"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            305..1873
                     /gene="cydA"
                     /locus_tag="ACHUPB_002856"
     CDS             305..1873
                     /gene="cydA"
                     /locus_tag="ACHUPB_002856"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0759619.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1889..3028
                     /gene="cydB"
                     /locus_tag="ACHUPB_002857"
     CDS             1889..3028
                     /gene="cydB"
                     /locus_tag="ACHUPB_002857"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0759620.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3043..3156
                     /gene="cydX"
                     /locus_tag="ACHUPB_002858"
     CDS             3043..3156
                     /gene="cydX"
                     /locus_tag="ACHUPB_002858"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0759621.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3156..3449
                     /gene="ybgE"
                     /locus_tag="ACHUPB_002859"
     CDS             3156..3449
                     /gene="ybgE"
                     /locus_tag="ACHUPB_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0759622.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3599..4003
                     /gene="ybgC"
                     /locus_tag="ACHUPB_002860"
     CDS             3599..4003
                     /gene="ybgC"
                     /locus_tag="ACHUPB_002860"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0759623.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            4000..4692
                     /gene="tolQ"
                     /locus_tag="ACHUPB_002861"
     CDS             4000..4692
                     /gene="tolQ"
                     /locus_tag="ACHUPB_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0759624.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4696..5124
                     /gene="tolR"
                     /locus_tag="ACHUPB_002862"
     CDS             4696..5124
                     /gene="tolR"
                     /locus_tag="ACHUPB_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0759625.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5189..6421
                     /gene="tolA"
                     /locus_tag="ACHUPB_002863"
     CDS             5189..6421
                     /gene="tolA"
                     /locus_tag="ACHUPB_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0759626.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6554..7846
                     /gene="tolB"
                     /locus_tag="ACHUPB_002864"
     CDS             6554..7846
                     /gene="tolB"
                     /locus_tag="ACHUPB_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0759627.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7881..8402
                     /gene="pal"
                     /locus_tag="ACHUPB_002865"
     CDS             7881..8402
                     /gene="pal"
                     /locus_tag="ACHUPB_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0759628.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8412..9203
                     /gene="cpoB"
                     /locus_tag="ACHUPB_002866"
     CDS             8412..9203
                     /gene="cpoB"
                     /locus_tag="ACHUPB_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0759629.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9368..9443
                     /locus_tag="ACHUPB_002867"
     tRNA            9368..9443
                     /locus_tag="ACHUPB_002867"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9401..9403,aa:Lys,seq:ttt)
     gene            9593..9668
                     /locus_tag="ACHUPB_002868"
     tRNA            9593..9668
                     /locus_tag="ACHUPB_002868"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9626..9628,aa:Val,seq:tac)
     gene            9672..9747
                     /locus_tag="ACHUPB_002869"
     tRNA            9672..9747
                     /locus_tag="ACHUPB_002869"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9705..9707,aa:Lys,seq:ttt)
     gene            9796..9871
                     /locus_tag="ACHUPB_002870"
     tRNA            9796..9871
                     /locus_tag="ACHUPB_002870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9829..9831,aa:Lys,seq:ttt)
     gene            9905..9980
                     /locus_tag="ACHUPB_002871"
     tRNA            9905..9980
                     /locus_tag="ACHUPB_002871"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9938..9940,aa:Lys,seq:ttt)
     gene            10005..10136
                     /locus_tag="ACHUPB_002872"
     ncRNA           10005..10136
                     /ncRNA_class="other"
                     /locus_tag="ACHUPB_002872"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10385..>11261
                     /gene="nadA"
                     /locus_tag="ACHUPB_002873"
     CDS             10385..>11261
                     /gene="nadA"
                     /locus_tag="ACHUPB_002873"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0759630.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2774 a         2703 c         3022 g         2762 t
ORIGIN      
        1 gagataaatt gttctcgatc aaattggctg aaaggcggta atttagctat aaattgatca
       61 ccgtcgaaaa atgcaaattt gcttcaacaa aaacctgttt attgtaagga ttttgcagcg
      121 taatatatac gtgggatcaa tttgagtttt tattaacatg tttgcaacct ttctttacgc
      181 cgtttttgtg tgcattcaca tggtatgatg aaagtgttca aacaaatttc tattggggca
      241 tgcgtgtgac cctttctaac ggggttcact ctcggagtct tcatgcgatg agcaaggagt
      301 catgatgtta gatatagtcg aactgtcgcg cttacagttt gccttgaccg cgatgtacca
      361 cttccttttt gtgccactga cgctcggtat ggcgttcctg ctggccatta tggaaacggt
      421 ctacgtcctc tccggcaaac agatttataa agatatgacc aagttctggg gcaagttgtt
      481 tggtatcaac ttcgctctgg gtgtggctac cggtctgacc atggagttcc agttcgggac
      541 taactggtct tactattccc actatgtagg ggatatcttc ggtgcgccgc tggcaatcga
      601 aggtctgatg gccttcttcc tcgaatccac ctttgtaggt ctgttcttct tcggttggga
      661 tcgtctgggt aaagttcagc atatgtgtgt cacctggctg gtggcgctcg gttcaaacct
      721 gtccgcactg tggattctgg ttgcgaacgg ctggatgcaa aacccaatcg cgtccgattt
      781 caactttgaa actatgcgta tggagatggt gagcttctcc gagctggtgc ttaacccggt
      841 tgctcaggtg aaattcgttc acactgtagc gtctggttat gtgactggcg cgatgttcat
      901 cctcggtatc agcgcatggt atatgctgaa aggtcgtgac ttcgccttcg ctaaacgctc
      961 ctttgctatc gctgccagct tcggtatggc tgctgttctg tctgttattg ttctgggtga
     1021 tgaatccggc tacgaaatgg gcgacgtgca gaaaaccaaa ctggctgcta ttgaagccga
     1081 gtgggaaacg caacctgcgc ctgctgcctt tactctgttc ggcattcctg atcaggaaga
     1141 ggagacgaac aaatttgcga ttcagatccc ttacgcactg ggcatcattg caacgcgttc
     1201 cgtggatacc ccggttatcg gcctgaaaga gctgatggtg cagcatgaag aacgcattcg
     1261 taacgggatg aaggcgtact ctctgctcga acaactgcgt tctggttcta ccgaccaggc
     1321 ggttcgtgac cagttcaata gcatgaagaa agacctcggt tacggtctgc tgctgaaacg
     1381 ctatacgcca aacgtggctg atgcgactga agcgcagatt caacaggcaa ccaaagactc
     1441 catcccgcgt gtagcgccgc tgtactttgc gttccgtatc atggtggcgt gtggcttcct
     1501 gcttctggca atcatcgcgc tctctttctg gagtgtcatc cgcaaccgca ttggcgagaa
     1561 aaaatggctt ctgcgcgccg cgctgtacgg tattccgctg ccgtggattg ctgtagaagc
     1621 gggctggttc gtggctgaat atggccgcca accgtgggct atcggtgaag tgctgccgac
     1681 agctgtggcg aactcgtcac tgaccgcagg cgatctcatc ttctcaatgg tgctgatttg
     1741 cggcctgtat accctgttcc tggtggcaga attgttctta atgttcaagt ttgcacgcct
     1801 cggcccaagc agcctgaaaa ccggtcgcta tcactttgag cagtcttcca cgactactca
     1861 gccggcacgc taagacagga gtcgtcaaat gatcgattat gaagtattgc gttttatctg
     1921 gtggctgctg gttggcgttc tgctgattgg ttttgcagtc actgacggtt tcgacatggg
     1981 ggtgggcatg ctcacccgtt tcctcggtcg taacgacacc gagcgtcgaa ttatgattaa
     2041 ctccattgca ccacactggg acggtaacca ggtttggctg atcaccgcgg gcggcgcact
     2101 ctttgctgcc tggccgatgg tctatgccgc tgcgttctcc ggcttctatg tggcgatgat
     2161 cctcgtgctg gcgtctttgt tcttccgtcc ggtcggtttt gactaccgct ccaagattga
     2221 agaaacccgc tggcgtaaca tgtgggactg gggcatcttc attggtagtt tcgttccgcc
     2281 gctggtaatt ggtgtagcgt tcggtaacct gttgcagggc gtaccgttca acgttgatga
     2341 atatctgcgt ctgtactaca ccggtaactt cttccagttg cttaacccgt tcggcctgct
     2401 ggcaggcgtg gtgagcgtag ggatgatcat tactcagggc gcaacctatc tgcaaatgcg
     2461 taccgtgggc gaactgcacc tgcgtacccg tgcaacggct caggtggctg cgctggtgac
     2521 actggtctgt ttcgcactgg ctggcgtatg ggtgatgtac ggtatcgatg gttatgtcgt
     2581 gaaatcgaca atggaccatt acgcagcctc taacccactg aataaagaag tggttcgtga
     2641 agctggcgca tggctggtta acttcaacaa cacgccaatt ctgtgggcta ttccggcact
     2701 gggtgtggtt cttccgctgc tgaccatcct gactgcacgt atggataaag ccgcgtgggc
     2761 gtttgtgttc tcctccctga cgctggcctg catcatcctg acagccggta tcgcaatgtt
     2821 cccgtttgtg atgccgtcca gcaccatgat gaacgcaagt ctgacaatgt gggatgcaac
     2881 ttccagccag ctgacgctta acgtcatgac ctgggttgcg gtggttctgg taccgatcat
     2941 tctgctctac accgcttggt gttactggaa aatgttcggt cgtatcacca aagaagatat
     3001 tgaacgtaac acccactctc tgtactaagt aaggagctaa aaatgtggta tttcgcatgg
     3061 attctgggaa cgcttcttgc ctgttcgttt ggggtaatca ccgcgctggc gcttgaacac
     3121 gtcgaatcag gcaaagccgg tcaagaagac atctgatgag taagattatc gcgaccttgt
     3181 atgcggtaat ggacaagcgc cccctgcggg cgctttcctt cgtgatggcg cttctgttag
     3241 caggatgtat gttttgggac ccatctcgtt ttgccgcgaa gaccagtgaa ctggaaatct
     3301 ggcatggttt attgctgatg tgggccgtct gtgctggtgt gattcacggc gtgggctttc
     3361 gtccgcagaa ggttctttgg caagggattt tttgcccatt gcttgccgat attgttctca
     3421 ttgtcgggct gattttcttc ttcttttaaa tcagaatttt ctttaaaaaa ttatgggccg
     3481 ctccaggccc ataaattttt acgctccctt aacttgccct cattcccaaa cctcaatcgc
     3541 gcgcgtatag tagcagcgtt taaaagctct aacttttgtt gcattaccgg gatgtaaagt
     3601 gaatacaacg ctgtttcgat ggccggttcg cgtctactat gaagataccg atgccggtgg
     3661 tgtggtgtac cacgccagtt acgtcgcttt ttatgaaaga gcacgcacag agatgctgcg
     3721 tcatcatcac ttcagtcagc aggcgctgat ggctgaacgc gttgcctttg tggtacgtaa
     3781 aatgacggtg gaatattacg cacctgcgcg gctcgacgat atgctcgaaa tacagactga
     3841 aataacatca atgcgtggca cctctttggt tttcacgcaa cgtattgtca acgccgagaa
     3901 tactttgctg aatgaagcag aggttctggt tgtttgcgtt gacccactca aaatgaagcc
     3961 ttgtgcgctt cccaagtcta ttgtcgcgga gtttaagcag tgactgacat gaatatcctt
     4021 gatttgttcc tgaaggctag ccttctggtt aaacttatca tgttgatttt gattggtttt
     4081 tcaatcgcat cctgggccat tattatccag cggacccgta ttcttaacgc agcggcgcgc
     4141 gaagccgaag cgtttgaaga taaattctgg tctggaatcg aactctctcg cctctatcaa
     4201 gagagtcagg ggaaacggga taatctgact ggttcggaac aaatatttta cagcgggttc
     4261 aaagagtttg tgcgcctgca tcgtgccaat agccatgcgc cggaagccgt agtggaaggg
     4321 gcgtcgcgtg ctatgcgtat ctccatgaac cgtgaacttg aaaatctgga aacgcacatt
     4381 ccgttcctcg gtacggttgg ctccatcagc ccgtatattg gtctgtttgg tacggtctgg
     4441 gggatcatgc acgcctttat cgccctcggg gcggtaaaac aagcaacact gcaaatggtt
     4501 gcgcccggta tcgcagaagc gttgattgcg actgcaattg gtctgttcgc cgcaattccg
     4561 gcggtaatgg cttataaccg cctcaaccag cgcgtaaaca aactggaact gaattacgac
     4621 aactttatgg aagagtttac cgcgattctg caccgccagg cgtttaccgt tagcgagagc
     4681 aacaaggggt aagccatggc cagagcgcgt ggacgaggtc gtcgcgatct caagtccgaa
     4741 atcaacattg taccgttgct ggacgtactg ctggtgctgt tgctgatctt tatggcgaca
     4801 gcgcccatca tcacccagag cgtggaggtc gatctgccag acgctactga atcacaggcg
     4861 gtgagcagta acgataatcc gccagtgatt gttgaagtgt ctggtattgg tcagtacacc
     4921 gtggtggttg agaaagatcg cctggagcgt ttaccaccag agcaggtggt ggcggaagtg
     4981 tccagccgtt tcaaggccaa cccgaaaacg gtctttctga tcggtggcgc aaaagatgtg
     5041 ccttacgatg aaataattaa agcactgaac ttgttacata gtgcgggtgt gaaatcggtt
     5101 ggtttaatga cgcagcctat ctaaacatct gcgtttccct tgcttgaaag agagcgggta
     5161 acaggcgaac agtttttgga aaccgagagt gtcaaaggca accgaacaaa acgacaagct
     5221 caagcgggcg ataattattt cagcagtgct gcatgtcatc ttatttgcgg cgctgctctg
     5281 gagttcgttc gatgagaata tagaagcttc agccggaggc ggcggtggtt cgtccatcga
     5341 cgctgtcatg gttgattcag gtgcggtagt tgagcagtac aaacgcatgc aaagccagga
     5401 atcaagcgcg aagcgttctg atgagcagcg caagataaag gaacagcagg ctgctgaaga
     5461 actgcgtgag aaacaagcgg ctgaacagga acgcctgaag caacttgaga aagagcggtt
     5521 agctgctcag gaacagaaaa agcaggctga agaagccgca aaacaggccg agttaaagca
     5581 gaagcaagcg gaagaggcgg cagcgaaagc ggcggcagat gctaaagcga aggccgaagc
     5641 ggatgctaaa gctgcggaag aagcagcgaa gaaagcggcg gcagacgcga agaaaaaagc
     5701 agaagcagaa gccgccaaag ccgcagccga agcgcagaaa aaagccgagg cagccgctgc
     5761 ggcactgaag aagaaagcgg aagcggcaga agctgctgca gctgaagcaa gaaagaaagc
     5821 ggcagcagag aaagctgcag ccgacaaaaa agcagcagaa aaagcggctg ctgataaggc
     5881 agcagctgat aagaaagcag cggcagaaaa agccgccgca gacaaaaaag cggcagcggc
     5941 aaaagcagcg gcagaaaaag ccgccgcaga caaaaaagcg gcagcggcaa aagcagcagc
     6001 tgaaaaagcc gctgcagcaa aagctgccgc agaggcagat gatattttcg gtgagctaag
     6061 ctctggtaag aatgcaccga aaacgggggg aggggcgaaa gggaacaatg cttcgtctgc
     6121 cgggagtggt aatactaaaa acaatggcgc atcaggggcc gatatcaata actatgccgg
     6181 gcagattaaa tctgctatcg aaagtaagtt ctatgacgca tcgtcctatg caggcaaaac
     6241 ctgtacgctg cgcataaaac tggcacccga tggtatgtta ctggatatca aacctgaagg
     6301 tggcgatccc gcactttgtc aggctgcgtt ggcagcagct aaacttgcga agatcccgaa
     6361 accaccaagc caggcagtat atgaagtgtt caaaaacgcg ccattggact tcaaaccgta
     6421 atcgcgatgt tgactgttcc gacggtcaac atcaggcacc ggttgccacg gggttctggt
     6481 agttttgtgt attttagttt gttaacattc tgctaaatta tcgtgggcca tcggtccaga
     6541 taagggagat atgatgaagc aggcattacg agtagcattt ggttttctca tactgtgggc
     6601 atcagttctg catgctgaag tccgcattgt gatcgacagc ggtgtagatt ccggtcgtcc
     6661 tattggtgtt gttcctttcc agtgggcggg ccctggtgcg gcacctgaag atattggcgg
     6721 catcgttgct gccgacttgc gtaacagcgg taaatttaat ccgttagatc gcgctcgtct
     6781 gccacagcag ccgggtagtg cgcaggaagt acaaccagct gcatggtccg cgctgggtat
     6841 tgacgctgta gttgtcggtc aggtcactcc gaatccggat ggttcttaca atgttgctta
     6901 tcaacttgtt gacactggcg gcgcaccggg tactgtactt gctcagaact cgtacaaagt
     6961 gaacaagcag tggctgcgct atgctggtca taccgccagt gatgaagtgt ttgaaaaact
     7021 gaccggcatt aaaggtgcgt tccgtacccg tattgcctac gttgttcaga ccaacggcgg
     7081 tcagttcccg tatgaactgc gcgtatctga ctatgacggt tacaaccagt ttgtcgttca
     7141 ccgttcacca cagccgctga tgtctccggc gtggtcacca gacggttcta aactggctta
     7201 tgtgaccttc gaaagcggtc gttccgcgct agttatccaa acgctggcaa acggcgctgt
     7261 acgtcaggtg gcttcattcc cgcgtcacaa cggtgcgcct gcattctcgc cagacggcag
     7321 caaactggca ttcgccttgt cgaaaaccgg tagcctgaac ctgtacgtaa tggatttggc
     7381 ttctggtcag atccgccagg tgactgatgg tcgcagtaac aataccgaac cgacctggtt
     7441 cccggacagc cagaacctgg catttacttc tgaccaggcc ggtcgtccac aggtttataa
     7501 agtgaatatc aacggcggtg cgccacaacg tattacctgg gaaggttcgc agaaccagga
     7561 tgcggatgtc agcagcgacg gtaaatttat ggtaatggtc agctccaacg gtgggcagca
     7621 gcacattgcc aaacaagatc tggcaacggg aggcgtacaa gttctgtcgt ccacgttcct
     7681 ggatgaaacg ccaagtctgg cacctaacgg cactatggta atctacagct cttctcaggg
     7741 gatgggatcc gtgctgaatt tggtttctac agatgggcgt ttcaaagcgc gtcttccggc
     7801 aactgatgga caggtcaaat tccctgcctg gtcgccgtat ctgtgataat aattaattga
     7861 atagtaaagg aatcattgaa atgcaactga acaaagtgct gaaagggctg atgattgctc
     7921 tgcctgttat ggcaattgcg gcatgttctt ccaacaagaa cgccagcaat gacggcagcg
     7981 aaggcatgct gggtgccggc actggtatgg atgcgaacgg cggcaacggc aacatgtctt
     8041 ccgaagagca ggctcgtctg caaatgcaac agctgcagca gaacaacatc gtttacttcg
     8101 atctggacaa gtacgatatc cgttctgact tcgctcaaat gctggatgca catgcaaact
     8161 tcctgcgtag caacccgtct tacaaagtca ccgtagaagg tcacgcggac gaacgtggta
     8221 ctccggaata caacatctcc ctgggtgaac gtcgtgcgaa cgccgttaag atgtacctgc
     8281 agggtaaagg cgtttctgca gaccagatct ccatcgtttc ttacggtaaa gaaaaacctg
     8341 cagtactggg tcatgacgaa gcggcatact ccaaaaaccg tcgtgcggta ctggtttact
     8401 aagagaattg catgagcagt aacttcagac atcaactatt gagtctgtcg ttactggttg
     8461 gtatagcggc cccctgggcc gcttttgctc aggcaccaat cagtagtgtc ggctcaggct
     8521 cggtcgaaga ccgcgtcact caacttgagc gtatttctaa tgctcacagc cagcttttaa
     8581 cccaactcca gcaacaactt tctgataatc aatccgatat tgattccctg cgtggtcaga
     8641 ttcaggaaaa tcagtatcaa ctgaatcagg tcgtggagcg gcaaaagcag atcctgttgc
     8701 agatcgacag cctcagcagc ggtggtgcag cggcgcaatc aaccagcagc gatcaaagcg
     8761 gtgcggcggc atcaacgacg ccgacagctg atgctggtac tgcgaatgct ggcgcgccgg
     8821 tgaaaagcgg cgatgcaaac acggattaca atgcagctat tgcgctggtg caggataaat
     8881 cccgccagga tgacgcaatg gtggcatttc agaatttcat caaaaattac cctgattcaa
     8941 cttacctgcc aaacgccaat tattggctgg gtcagttaaa ctacaacaag ggtaaaaaag
     9001 atgatgcggc gtactatttt gcttcggtag tgaaaaacta tccgaagtca ccaaaggctg
     9061 ctgatgcgat gtttaaagtc ggcgtcatca tgcaggacaa aggtgacacc gcaaaagcga
     9121 aagccgtata ccagcaggtt atcagtaaat accctggtac tgatggcgct aaacaggcac
     9181 aaaaacgtct gaacgcgatg taatgcataa cacacgacca gaagtcgcat tatttctggt
     9241 cgtgtcgtgc gaatcataag cagttgagtg atctgcatcg aaatttttgt tgcgctcaag
     9301 tctgaaatca gtaatatatg ccgccgttgc cacgggatat caaacaaacc gaaagcaacg
     9361 aaaaagtggg tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg
     9421 ttcgaatcct gcacgaccca ccagttttag catcgaagac agatgttaag cgtgtaggat
     9481 aacgttgcgt cagcaacggc ccgtagggcg agcgaagcga gtcatcctgc acgacccacc
     9541 actaattacg gtgggttcgg tggaagtagt ttgtagtatc cagcgcagta tcgggtgatt
     9601 agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc
     9661 acccaccact cgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg
     9721 caggttcgaa tcctgcacga cccaccaatc ttaaagattg gccccgagta gaaatctttc
     9781 aggtaacacc cgtatgggtc gttagctcag ttggtagagc agttgacttt taatcaattg
     9841 gtcgcaggtt cgaatcctgc acgacccacc atcctgaatg attaaagcag cataatcccg
     9901 caaggggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc
     9961 gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctga
    10021 gatattcaaa gattcgaacc tgcagcaggt tcgagttgag cgcagcgaaa caacggagcc
    10081 gctcgcggcg acggcccgaa gggcgagcga agcgagtcat cctgcacgac ccaccaatgt
    10141 aaaaaagcgc cctaaaggcg cttttttgct attcaggcat cctcaatttc actttgtaaa
    10201 cctgatgaca tcgtcagagc ttactgtgca agcaactcta cgtcggtgga attaggcgta
    10261 attcgagtga cttttcccca ccattcgact atcttgttta gcatataaaa caaattacgc
    10321 cgatagtagc gaatattacg ctaatgtcgg ttttaacgtt aagcctgtaa aacgagatgg
    10381 taagatgagc gtgatgtttg atccagacac ggcgatttat cctttccccc cgaagccgac
    10441 gccgttaagc attgatgaaa aagcgtatta ccgcgagaag ataaaacgtc tgctaaaaga
    10501 acgtaatgcg gtgatggttg cccactacta taccgacccc gaaattcaac aactggcaga
    10561 agaaaccggt ggctgtattt ctgattctct ggaaatggcg cgcttcggtg caaagcatcc
    10621 cgcttctacg ttgttagtcg ctggggtgag atttatggga gaaaccgcca aaattctcag
    10681 tccggaaaaa acaattctga tgctgacact tcaggctgaa tgttcactgg atctcggctg
    10741 ccctgttgaa gaatttaacg cattttgcga tgcccatccc gatcatactg tcgtcgtcta
    10801 cgccaacact tctgctgcgg taaaagcgcg cgcagattgg gtggtaactt caagcattgc
    10861 cgtcgaactt attgatcatc tggatagttt tggtgaaaaa atcatctggg cacccgacaa
    10921 acatctgggg cgttacgtgc aaaaacagac gggcgcagac attttatgct ggcagggtgc
    10981 ctgtattgtg catgatgaat ttaagactca ggcgttaacc cgcttgcaag aagaataccc
    11041 ggatgctgcc atactggtgc atccagaatc accacaagct attgtcgata tggcggatgc
    11101 ggtcggttcc accagtcaac tgatcgctgc tgcgaaaaca ttgccacatc agcggcttat
    11161 cgtggcaacc gatcggggca ttttctacaa aatgcagcag gcggtgccag ataaagagtt
    11221 actggaagca ccaaccgcag gtgagggtgc aacctgccgc a
//
LOCUS       DAZOZD010000135        11011 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOZD010000135 DAZOZD010000000
VERSION     DAZOZD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11011
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.135"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            150..764
                     /gene="evgA"
                     /locus_tag="ACHUPB_002874"
     CDS             150..764
                     /gene="evgA"
                     /locus_tag="ACHUPB_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0759631.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            769..4362
                     /gene="evgS"
                     /locus_tag="ACHUPB_002875"
     CDS             769..4362
                     /gene="evgS"
                     /locus_tag="ACHUPB_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0759632.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4418..5563)
                     /gene="yfdE"
                     /locus_tag="ACHUPB_002876"
     CDS             complement(4418..5563)
                     /gene="yfdE"
                     /locus_tag="ACHUPB_002876"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0759633.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5637..6581)
                     /gene="yfdV"
                     /locus_tag="ACHUPB_002877"
     CDS             complement(5637..6581)
                     /gene="yfdV"
                     /locus_tag="ACHUPB_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0759634.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6651..8345)
                     /gene="oxc"
                     /locus_tag="ACHUPB_002878"
     CDS             complement(6651..8345)
                     /gene="oxc"
                     /locus_tag="ACHUPB_002878"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0759635.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8399..9649)
                     /gene="frc"
                     /locus_tag="ACHUPB_002879"
     CDS             complement(8399..9649)
                     /gene="frc"
                     /locus_tag="ACHUPB_002879"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0759636.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10162..10785)
                     /locus_tag="ACHUPB_002880"
     CDS             complement(10162..10785)
                     /locus_tag="ACHUPB_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0759637.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3227 a         2482 c         2256 g         3046 t
ORIGIN      
        1 atttatgccg actatttata tggtatactt gtcgaattat cttaaaggaa gctcagattt
       61 tcttattttt attgagaaaa tgagatgacg ccttatgtct gtattactac agggagaagg
      121 gagatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat gaccatcctc
      181 ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc ttagcggagt
      241 taactgaagg cggaagcgcc gttcagcggg tggaaacact taagcctgat atcgtcatca
      301 ttgatgtcga tatccccgga gttaacggta tcctggtgtt agaaacgctg aggaagcgcc
      361 aatatagcgg aattattatt atcgtctccg ctaaaaatga ccatttttac gggaaacatt
      421 gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac aatatcattg
      481 cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc aaccggtttg
      541 ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa gaaattagtg
      601 tcatgcggta tattcttgat ggcaaggata ataatgacat tgcggaaaaa atgttcatca
      661 gcaacaaaac tgtcagcact tataaaagtc gcctgatgga aaaattagaa tgtaaatcac
      721 tgatggatct ttacacattc gcacaacgta acaaaatcgg ctaaccacat gaagttttta
      781 ccctatattt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc agacgaagat
      841 tacatcgaat atcgtggcat cagtagtaac aaccgtgtca tacttgatcc agtacgtctg
      901 agcaacaagg aattacgttg gttagcgagc aaaaaaaatc ttgtgattgc agtacataag
      961 tcccaaacgg ctacgttgtt gcataccgat tcgcagcaac gagttcgtgg tattaatgct
     1021 gattatttaa atcttttaaa aagaacgtta aatatcaaat taacactccg ggaatacgcc
     1081 gatcatcaaa aagcaatgga cgcgctggaa gaaggtgaag tcgatatagt gttatcacat
     1141 ttagtagctt cgccgcctct taatgatgac attgctgcaa ccaaaccact gataattacc
     1201 tttccggcgc tggtaaccac ccttcacgat tcaatgcgac cgcttacctc atcaaaacca
     1261 gtaaatattg ctcgagtagc aaattatccc cctgacgagg taattcatca atcatttcca
     1321 aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt ctcagccgga
     1381 cagaatgatt actttattgg tagtaacatc attaccagca gtatgatttc ccgctatttc
     1441 actcactcct taaatgtagt gaaatattat aactcaccgc gtcaatataa ttttttcttg
     1501 accagaaaag aatctgtcgt tcttaatgaa gtactcaatc gatttgttga tgctttgaca
     1561 aatgaagttc gctatgaagt atcacaaaat tggcttgata caggaaacct ggcctttctg
     1621 aacaaaccat tagaactcac tgaacatgaa aaacaatgga ttaagcagca tcccgattta
     1681 aaggtgctgg aaaatcctta ctcccctccc tattctatga cggatgaaaa tggctcggtt
     1741 cggggcgtta tgggggatat tcttaatatt attactttgc aaacaggttt aaatttttct
     1801 ccgatcaccg tttcacacaa tatccatgct ggaacacagc tgaatcccgg cggatgggat
     1861 atattacccg gtgctattta tagtgaagat agagaaaata atgttttatt tgctgaagcc
     1921 ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga acaaacatta
     1981 aaaaaaggaa tgaaagttgc cattccatat tattatgagc ttcattcgca attaaaagag
     2041 atgtatccgg aggtagagtg gataaaagtc gataacgcca gcgctgcatt tcacaaggtc
     2101 aaggaaggcg aacttgatgc tctggtcgcg acacagttaa attcacgtta catgatcgac
     2161 cattactatc ctaatgaact ttatcatttt cttattcccg gcgttccgaa tgcatcgctt
     2221 tcgttcgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa agcactgaat
     2281 gcaatccccc caagcgaagt tctgcgcctg accgaaaaat ggattaaaat gcccaatgtg
     2341 accattgaca catgggacct ctatagcgag caattttata ttgttacgac attatccgtt
     2401 ttattagttg gcagtagcct tttatgggga ttttacctgt tacgctcagt tcgtcgtcgt
     2461 aaagtcattc agggtgattt agaaaaccaa atatcattcc gaaaagcact ctcggattcc
     2521 ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca taatagtgct
     2581 tttgaacatt atttcactgc ggattactac aaaaatgcaa tgttaccatt agaaaacagt
     2641 gactccccct ttaaagatgt tttttctaat gcgcatgaag tcacagcaga aacgaaagaa
     2701 aatcgaacaa tatacacaca ggtttttgaa attgataatg gcatcgagaa aagatgcatt
     2761 aatcactggc atacattatg caatctgcca gccagcgatc atgctgttta tatttgtggt
     2821 tggcaagata ttacagagac gcgtgattta attcatgcac tcgaagtaga gagaaataaa
     2881 gcgatcaatg caactgtcgc aaaaagtcag tttctggcaa caatgagtca cgaaataaga
     2941 acaccaataa gctccattat gggcttcctg gaacttctgt cgggttctgg tcttagcaag
     3001 gagcaacggg tggaggcgat ttcacttgcc tactccaccg gacaatcact cctcggctta
     3061 attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact tcaaccacaa
     3121 tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc gattgctgca
     3181 agcaaatcga tcgcattaat ttgcagcagt acatttcctg atcattacct ggttaagatc
     3241 gaccctcagg cgtttaagca ggtcttatca aatttactga gcaatgctct caaatttacc
     3301 accgaggggg cagtaaaaat tacgacctcc ctggttcaca ttgatgacaa ccacgctgtt
     3361 atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca acaacaactc
     3421 tttaaacgct acagccaaac aagtgcaggt cgccagcaaa caggatctgg cttaggctta
     3481 atgatctgca aagaattaat taaaaatatg cagggcgatt tgtcattaga aagtcatcca
     3541 ggcataggaa caacatttac gatcacaatc ccggtagaaa ttagccagca agtggcgact
     3601 gtcgaggcaa aagcagaaca ccccatcaca ctacctgaaa agttgagcat attaatcgcg
     3661 gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt aggatatgat
     3721 gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca acattatgat
     3781 ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac tcgcaaactc
     3841 cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca ggctaacgaa
     3901 cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt gaccctggat
     3961 gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc tcagtatcgc
     4021 caccttgata tcgaagccct gaaaaataat acggcgaacg atctacaact gatgcaggag
     4081 attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt tcaagcacta
     4141 gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg tgcggctaac
     4201 atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac acctgtttca
     4261 gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca cattgcagag
     4321 ctggaccagg agattgctgt tttctgtcag aaaaatgact aaatagcggc tcccacaatg
     4381 ttctaatgtg ggagctattt accagcacat cttacattta tgatgagaac tcctggcgaa
     4441 tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg tccgcgcagc
     4501 cgctgatttt tatcggatta cccggcatca ttattcctcc ggcttcaatc aacatatttc
     4561 tcgcctgagt ttgtggcaaa ttaatggcct cagccacact taataacggc gcgacgggca
     4621 caccaacttc atgtattttg gctaaccaaa cttcagctgc ctgcgttttt aacgttcgct
     4681 caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg ctaaatcggg
     4741 gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa agcttgtcat
     4801 taccacaaca aatcgtaatc ggcttatcct gagtatcgaa aacatcaaaa ggtgccatgt
     4861 agggatggcg atttcccagg cgttgtggtg acttccctgt cgcaatatat gccatcagac
     4921 catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc gcccctctct
     4981 ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag acaccgccgc
     5041 atagatccgc aagagaggta ccaacacgca ctggcggagc atcagggtac cctgtttcca
     5101 tcattatccc actcattgcc tgaatgatgg tatcgtaggc aggagcatct tttagcggac
     5161 cggtatgtcc gaaacctgac gatgaagcat atatgaggcg cgggttgatt tcttgtaacc
     5221 tttcccatga aaaccccagt ttttccattg tacctgggcg aaaattctca gctaatacat
     5281 cagcttgttt gagcatattt ataaatatac ttttatcgtg atcattcttt aaatcaagaa
     5341 ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca tccacatagg
     5401 gaccaaatgt gcgggtatca tcaccatgac ctggtggctc aactttaatt acccttgcgc
     5461 ccatattaca aagaagttga gttccgaaag gtccattaag gacatgtgtc atatcgataa
     5521 ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta tgccttgaaa
     5581 aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat gattaattat
     5641 gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaagcc cagtacgctc
     5701 acagccaatg acgctgtacc agtacgggtg tagacattaa accggctggc aataatgatt
     5761 ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcaaatg ttcgctgttc
     5821 aaatgacatg ccataccaac gagaagcagc gccagtggca tcagaatcaa cttcaggaag
     5881 gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt caaccccgca
     5941 gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggtcgg gtcccatgct
     6001 gccggaattt ttaccccaac cagcaccagg atcgttgcca gaacaggtgc ccatactact
     6061 ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc atccgctcct
     6121 gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac aataatagaa
     6181 ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc gagaactgca
     6241 aaccccagga atccaatggt aggtgaaccc gcaattaatg cacatacagc ggcttctgca
     6301 tgggtacgtt taaaaaattt gtagcaacca aaccaagaga aaaagaaaca tccgacaata
     6361 accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct gtttgcccgg
     6421 gtaatagaaa caaatagagc cgcaggaaga gcgtagttta ataccagttt attaaatgcg
     6481 cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa cagcatgatt
     6541 acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt tccgataagg
     6601 ctcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat ttaattacca
     6661 gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc agcagggtcg
     6721 atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa agcatgacga
     6781 agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat taatttgtca
     6841 taccttgcat ggtgcaacag atccgttggt gatggtgcac cagcgccact gagatcaaca
     6901 ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg caggttatat
     6961 cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc ttcaatggcg
     7021 acaaccggag aaccagaagt cacgctagca ccgatggcat agcccatacc gatgcccatg
     7081 acaccccagg taccacaatc cagacgacga cgtggtttat acatatcaat aatatttcgt
     7141 gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt ctcgcgcaat
     7201 acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc tgtacttaat
     7261 ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc gcgccatacc
     7321 agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat actggatgca
     7381 atatcaccaa cgactggcac agcaatgggg cggttgctgt caatttcctg cggttcaata
     7441 tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc caataaccaa
     7501 ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa cgaacgcgca
     7561 gctgccgcag aaagtggatg cgtatcttca aggatccctt tcgccataga cattggcagg
     7621 aatggaatct gagcactttc aataaattca cgaagctgtt catcagcttg tgaatacgcc
     7681 gcgcctttgc caaggataat taatggccgt tcagcttttg ctaaaagcga aattgcgcta
     7741 gtgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt aacaatcgtg
     7801 gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa atcaagataa
     7861 actccgccag ggcgacccga tacagagacc cggatagcgc gtgccaatgc aatgccaaga
     7921 tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc attcatttgg
     7981 tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct ggagccgcta
     8041 atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa accattgagg
     8101 aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa accgcttgcc
     8161 gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat gccttccgcc
     8221 tgggcatggc gtgccatatc cgtcacagga atacctacaa caccataaat agtgtcaata
     8281 ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg aagttgatct
     8341 gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc cgttaatatc
     8401 agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc agctcctgca
     8461 atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc gtaaaggcag
     8521 agaatttcat tggacaacca acggtcagat attttccacg caacggttgt tccacttcaa
     8581 caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata cttaaaaccg
     8641 gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta tcaatagtga
     8701 cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg gcatgggctg
     8761 tactgtatgc cgaatcggta atccattctg gtttgccgat ggctttacag gtgttttccc
     8821 agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc cagcctttac
     8881 atttcaggat ccagccaggc tgaccgccac cacctgcatt accaccgcgg ggaactgcat
     8941 caccaaatgt accattcgga tactgcgggt attcttccag ataacccaat ttatcgagac
     9001 gctgctggtc acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc attgacatgg
     9061 tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt aaaccgatca
     9121 gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc ggcggaccat
     9181 cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg agcaacgttt tcataggctt
     9241 ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa atcagacgtg
     9301 gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc cctggatgaa
     9361 agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct ttgccttccg
     9421 ctgttttggt atttaactca atagaacgtt tgttactgtt aagcatggtg aagtaaagcg
     9481 catcgatatc aggaatatct cgcagttggt gacgcgttac gtcaccaacg ccgggacgtt
     9541 caattttaat aacgtcagcg ccaaaccagg ccagcatttg agtacaagat gggccagatt
     9601 gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata gcaataccta
     9661 ttagttaatt aaatatgtgt taatttagcg tttatttaag gcataacgcc ctctataaat
     9721 aatctgcaaa atatcttcgt agcggcactc gatattttta aattcctgta tcaagtactg
     9781 aaaattattt catcgccact tccaattcct ttctgcatgg taagtagtga ggacgaaaag
     9841 aagaatatga taaatattta ttcgtggtaa tcagagagag tcgcgacatt tgtgatgtta
     9901 ttcttacaac tgtaagaagc gccggtgatt tacaagtaag agtatgatta cgctttttaa
     9961 agcgtaccat aaagcataac ataataaatg atttgtgatg acactggatt attgcggttt
    10021 actttgagga aaataaaatg cgtgtttata gaaccgcgag cgccaaataa ttttatgacc
    10081 acaacctgtc gtattgtggt cataaaaaat tgtcggctgg agggagttaa atcctttacc
    10141 tcaatatact gcctgtcagc attagttcac aaacagtgct tcgctatcga cgatgattcc
    10201 atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat caagcagttt
    10261 ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc atcagatatt ggttttccat
    10321 tacgccaaca ccggccaggc gaagctcttc cattgcgccc tttttatccc ctttcgccat
    10381 tttttcattg gcaattttta tcgcggcttc tttttcgggt gttgctacat aactctcaga
    10441 aattccgaca gatgcattaa tgacaatata ttggtcatca ttgagattgg tctttttacc
    10501 tggcttagcg aactttgccc attcggtact gtcatctgac agcaaagcgg aagcttcatt
    10561 ggtcagttct tttgcctttt ccggatcgcc atgaaacaga gccaggcgcg ctacctgcac
    10621 gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg ttttctgggt
    10681 ttgctgagtt tgctgttgtg ccgctaccgg tgcattatca gcagcccata cagttgaaga
    10741 tgccagaatt gctgtgacca tcgtagccat aattaaacgt ttcatactta cctcattctt
    10801 taacagtttt agaaaacgcc gccatcatat tgggtgagca tgaaatgaga aatcagagag
    10861 caacggtcat tttgaaaggg ttatcttaca attgtagtta ttatcgcaaa gcggcattta
    10921 gtcttacagt tgtaggaata tgcttcctgt cgcagagaac tattcttata taccacaaag
    10981 agatgtcatt tattatattg atgtaaatat t
//
LOCUS       DAZOZD010000136        11001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOZD010000136 DAZOZD010000000
VERSION     DAZOZD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.136"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..278)
                     /locus_tag="ACHUPB_002881"
     CDS             complement(<1..278)
                     /locus_tag="ACHUPB_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0759638.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(445..1185)
                     /locus_tag="ACHUPB_002882"
     CDS             complement(445..1185)
                     /locus_tag="ACHUPB_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0759639.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1278..1913)
                     /gene="argO"
                     /locus_tag="ACHUPB_002883"
     CDS             complement(1278..1913)
                     /gene="argO"
                     /locus_tag="ACHUPB_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0759640.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2052..2912)
                     /gene="mscS"
                     /locus_tag="ACHUPB_002884"
     CDS             complement(2052..2912)
                     /gene="mscS"
                     /locus_tag="ACHUPB_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0759641.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            3108..3206
                     /locus_tag="ACHUPB_002885"
                     /pseudo
     CDS             3108..3206
                     /locus_tag="ACHUPB_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="ACHUPB_002886"
     CDS             complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="ACHUPB_002886"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0759642.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="ACHUPB_002887"
     CDS             complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="ACHUPB_002887"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0759643.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5777..6796)
                     /gene="epd"
                     /locus_tag="ACHUPB_002888"
     CDS             complement(5777..6796)
                     /gene="epd"
                     /locus_tag="ACHUPB_002888"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0759644.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7168..7599
                     /locus_tag="ACHUPB_002889"
     CDS             7168..7599
                     /locus_tag="ACHUPB_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR0759645.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7622..8200
                     /locus_tag="ACHUPB_002890"
     CDS             7622..8200
                     /locus_tag="ACHUPB_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0759646.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8201..8908
                     /locus_tag="ACHUPB_002891"
     CDS             8201..8908
                     /locus_tag="ACHUPB_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR0759647.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8896..9573
                     /locus_tag="ACHUPB_002892"
     CDS             8896..9573
                     /locus_tag="ACHUPB_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0759648.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9567..10244
                     /locus_tag="ACHUPB_002893"
     CDS             9567..10244
                     /locus_tag="ACHUPB_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0759649.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10216..10929)
                     /locus_tag="ACHUPB_002894"
     CDS             complement(10216..10929)
                     /locus_tag="ACHUPB_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0759650.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10926..>11001)
                     /locus_tag="ACHUPB_002895"
     CDS             complement(10926..>11001)
                     /locus_tag="ACHUPB_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0759651.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2919 c         2740 g         2715 t
ORIGIN      
        1 cgtgaacgac gccctgagtt atggatttca ttatcaatcg cagaaagtcg ttgataaacc
       61 gggaacagtt tttgatacag ttcctgaccg gctttattga ggctgatatt attatctttg
      121 cgggtaaata gcgtatagcc aatttgttct tccagcgcgg caatgctttt accaaatggc
      181 gaagccgtca tatggatttt ttctgccgcc cgggcaatat tattggtttg cgctaataaa
      241 atgaaattgc gcatcttttt cgaaataaaa atgtccatcc cccctccccg catccttgtc
      301 cgcttaaccg tttcagtcag cctatccttg atgaaaccgc gagcaaagat aggtgattac
      361 gtcatggttt tacagaaaat tacagaaaaa ggaggcaata tcgggtaaag gcattagccc
      421 gacgaatacg tcgggctaca agtattattg tgctgcaggt gttttagcgg gttgttgatc
      481 cacaggttct aactggaaga ccacatcgac ctgatcatca aactgaatag cggcctgctc
      541 gtaagtttcc tgggcagaca ccggcgcggc atcggctttc atcatccgca ccattgggct
      601 gggctgatag ttggaaacat ggtagcgcac gctatatacc ggccccagtt tacgatgaaa
      661 gccgttcgcc agttcctgcg cctgatgaat cgcgttatca atcgctgcct tacgcgcttt
      721 gtctttatag gcatccggct gcgccacgcc cagcgacaca gaacgaattt cgttaagacc
      781 cgccttcagc gcgccatcca gcaaggaatt cagtttgtct aactgacgga gcgtgacttc
      841 caccgttctc acagcgcggt agcctttcag gatactttta ccatcctgat aatcataatc
      901 tggctgggtg cgtaagttcg ctgagctgat atcttttttc gcgatctgat tgagttcaag
      961 gaaggaaatg tattgtgcga cgcgctcatc tgcctgtttc ttggctgtag cggcatcctt
     1021 cgcggccacg ttaacttcaa tcgcaagagt ggcaatgtct ggcaccgcat ccacacttgc
     1081 cgtaccggag gtgacaatat gcggtccatc cggcaattcg ttagcctgcg ctgccatccc
     1141 gctaataccc attaatgccg ccagggcgat aactttgaac ttcacgttag tctctccgtg
     1201 ttaattgact tgcccaaaat tcaggacctc tggaggcaag cttagcgcct ctgttttatt
     1261 tttccatcag atagtgccta actgaacaag gcttgtgcat gagcaatacc gtctctcgcc
     1321 agctgcaagg ctataaacca catgacacat cccactacca gattgataat gcgctgtgat
     1381 tttgccgtgc gcaggcgcgg tgccagccag gctgcgagaa tagccagacc aaagaaccac
     1441 aggaaagagg cgctaattgt cccgagcgca aaccaacgtt ttggttcgac gtcaagctgc
     1501 ccgccaaggc tgcccagtac aacaaaagta tccaggtaaa catgcggatt cagccaggtc
     1561 actgccagca tggtggcgat aattttccat ctgccttgct tcaggacttc ggcactagct
     1621 aactcaatat tactactcat cgctgtttta aaagcgccaa aaccatacca cagcaagaag
     1681 gctacgccgc cccaggtgac cagcgccagc aaccacggcg actgcatcaa taacgcgctg
     1741 ccaccaaaaa tcccggcgca aatcaggacc aaatcgctga tagcacaaag taaggcaatc
     1801 ataatgtggt actgacgacg aatgccctga ttcatcacaa aagcattttg tggaccgagc
     1861 ggcagaatca tagctgcccc aagagcaata ccttgaaagt aataagaaaa cacgttggat
     1921 attccgaatt aagtatctga tgcagagact atatcgcggg attgttatta gcggaaattg
     1981 ataatattaa ttggcaatca gaataactaa taagagagag gccagagagt attatctggc
     2041 ctcagcgttg attacgcagc tttgtcttct ttcacccgct taaagttcac atccatttgc
     2101 gggtacggga agctgatacc ggcggcatca aattcacgtt taatacgctc cagcacatcc
     2161 cagtacacgt tttgcagatc gccgctgttg ctccagacgc ggaccacgaa attaatcgac
     2221 gatgcaccaa gttcgttcag acgcacagtc atttcgcgat ctttcaaaat gcgatcttca
     2281 gactggataa tattggtcag gatctgctta acctgatcga tatcggaatc atacgccacg
     2341 ccaataataa attcgttacg gcgaactggc tcgcgagaga agttaataat atttccggca
     2401 ataattttac cgttcggaat aacgataatt ttaccgtctg cagtacgcat ggtggtggag
     2461 aaaatctgca cactcagcac agtaccggct acgccgccga gatcaacata ttctccggca
     2521 cggaacgggc ggaacatgac aagtaacacg ccagcggcca ggttagaaag tgacccctgc
     2581 aaagccagac caacagctaa gcctgcggca ccgagtacag caatgactga cgctgtttgt
     2641 acacccacgc gtcccagggc agcgattagc gtaaaggcga taataccgta acggactaat
     2701 gcagaaagaa aatcagcaac agtggcatcg attttacggg agatcatcag gcgattcacc
     2761 gcgttggaaa tcatccgcgc gataatcaaa ccaacgatga tgatcgcgag tgccgccacg
     2821 atgtttactg cataacttag cagcagcgcc tggttagcta ccagccagct tcccgcgccg
     2881 tttatgctat cgacaacatt caaatcttcc attcaatatt ccttttcaac tgactcaaaa
     2941 tggagaaata cactccgccg ccttatgacg ggcagtctga caagggtaaa caataaagca
     3001 taattttgcc aaatagatca cagatttctc atctgaatca ccttgctttt ggcgaaaaaa
     3061 tccccgcaga gcggggctca gacagatatc aatgtgcgct ttgtcatgcc ggatgcggcg
     3121 tgaacgcctt atccggccta caaaaacctg caaattcaat atattgcagg atacgtgtag
     3181 gcctgataag cgtagcgcat caggcaattt tgcttttggc gaaaaaaaga cccgcagagc
     3241 gggccttgag ataagcagaa aggaatatct tacagaacgt cgatcgcgtt cagttcctgg
     3301 aatgcttttt ccagacgagc gatcatcgaa gtctgaccgg cacgcagcca tacgcgcgga
     3361 tcgtagtatt tcttgttcgg ctgatcttcg cctttcgggt tacccaactg cccctgcaga
     3421 taagcttcgt tcgctttgta gtagttcaga acgccttccc aggttgccca ttgggtatcg
     3481 gtatcgatgt tcatttttac tacgccgtag cttacggagt ctttgatttc ctgagcagta
     3541 gaaccggaac caccgtggaa tacgaagttc aggctgttgt gcggcaggtt gtgtttcttg
     3601 gaaacatatt cctgagaatc acgcaggatg gtcggagtca gaaccacgtt acccggcttg
     3661 taaacaccgt gtacgttacc gaaggacgca gcgatggtga aacgcgggct gattttgctc
     3721 agttcggtgt atgcgtaatc aacgtcttcc ggctgggtgt acagtgcaga agcgtccatg
     3781 tggctgttgt ccacgccgtc ttcttcacca ccggtgcaac ccagttcgat ttccagagtc
     3841 atgccgattt tggacatgcg ctccaggtat ttagagcaga tttcgatgtt ctcttgcaga
     3901 gattcttcag acaggtcgat catgtgagaa gagaacagcg gcttaccggt agctgcgaag
     3961 tgtttttcac ccgcgtccag cagaccgtcg atccacggca gcagtttctt cgcgcagtgg
     4021 tcagtgtgca ggataaccgg aacaccataa tgttcagcca tctggtgaac gtgatgcgca
     4081 ccagagatcg cgcccaggat agcagcaccc tgcggaacgt cagatttcac gcctttacca
     4141 gcgataaagg aagcaccacc gttggagaac tgaacgataa ccggcgcttt aactttagca
     4201 gcggtttcca gtacggcgtt gatggagtca gtaccgacgc agtttactgc tggcagtgcg
     4261 aagttgtttt cttttgctac ctggaaaact ttctgtacgt catcaccagt gattacgcca
     4321 ggttttacga aatcaaaaat cttagacatg tctcttgtcc tgtatcgtcg ggccttatac
     4381 tcatcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt cacttactta
     4441 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagta
     4501 tgaaaaaatg cgtgctctaa aagcgcgctg aaacaagggc aggtttccct gccctgtgat
     4561 tttttacttc ttagcgcgct cttcgagcat cgctactgca ggcagtactt taccttccac
     4621 gaattcgagg aatgcgccgc cgccagtgga gatgtaggag attttgtcag caatgccgaa
     4681 caggtcgatt gctgccagag tgtcgccgcc gccagcgatg gagaacgctt cgctgtctgc
     4741 gatagcgtta gccacgattt cagtaccttt gcggaagttc gggaattcga acacgccaac
     4801 cggaccgttc cacagaatgg ttttcgcatt cttcaggatt tcagccagtt cctgagcgga
     4861 agcatcaccg atatccagga tctgctcgtc agctttcaca tcgttaacag atttcagggt
     4921 agccgatgca gtttcagaga actcggttgc tacgcgaaca tcagacggaa ccgggatgtt
     4981 gcaggtggtc agcagacgtt tagcttcgtc aaccaggtca gcttcgtaca gggatttacc
     5041 cacatcgtgg ccttgtgccg cgataaaggt gttagcgata ccaccaccaa caatcagctg
     5101 gtcagcgatt ttagacaggg agtccagaac ggtcagtttg gtagatactt tagaaccacc
     5161 aacgatagcc accatcgggc gagcaggttc tttcagtgct ttacccagcg cgtccagttc
     5221 agctgccaac agcgggcctg cgcacgcaac atcagcgaat ttaccgatac cgtgagtaga
     5281 agcctgcgcg cggtgagcag taccgaatgc gtccattacg aacacgtcac acagtgcagc
     5341 gtattttttg gacagggttt cgtcgtcttt cttctcgcct ttgttgaagc gaacgttttc
     5401 cagaacaacc agttcacctt cagcaacgtc aacgccgtcg aggtaatctt taaccagacg
     5461 aaccgggtta gacagtttgt ctttcaggta gttaacaacc ggcagcagag agaattcttc
     5521 gttgtactcg ccttcggtag gacgacccag gtgggaagtt accatcactt tcgcgccttg
     5581 tttcagggcc agttcgatgg tcggcagaga agcacggata cgcgcgtcgc tggttacttt
     5641 cccgtctttt actggtacgt tcagatccgc acggataaat acacgtttcc cagcaagatc
     5701 cagatcggtc atcttaatta cagacatggt gaatcctctc gttgattcta aaagttttgc
     5761 agacgctgct tgcgtcttac ctgaaagcaa cagtagccat agctaacgtc gtgtcgagca
     5821 ttcggttagc aaagccccat tcgttatcgc accagaccaa cgttttgatc aggtgtgcgc
     5881 cactgacccg ggtttgggtg ccatcgacaa tggcactgtg cggatcgtgg ttaaaatcta
     5941 cagagaccaa cggcaattcc gtatagtcaa ctataccatg aaatgcacct tgtgctgctt
     6001 tttgcagcaa caggttgact tcattggctt ttacaggttt cttcaccgtc acgcttaaat
     6061 cgattgccgt cacatttatg gttggcacac gtaccgcaat cgcttcaaag cgatcgttaa
     6121 attgcggaaa aaatcgtgtg ataccggcgg ccagtttagt atcgaccgga atgatcgact
     6181 ggctggctgc ccgggtgcga cgcaggtcag gatggtatgc atcaataacc tgttgatcgt
     6241 gcatggcgga gtgaattgtg gtcacagtgc cggactcaat accgtacgca tcatctaaca
     6301 atttgatgac gggaattatg caattcgtgg tacaggaagc gttagaaacg atgcggtgtt
     6361 ccgcacgaag ttgatcctga ttgacgccgt aaacaacggt cgcgtcgaga tcgttactgc
     6421 caggatgtga aaagagcact tttttggccc cggcggcaat atgcgcttcg ccatgctcgc
     6481 gggagccata tacgccggtg cagtcgagga ctacatcaac gccaagttca cgccagggga
     6541 gcgattgcag tgaacgttca tgcaatacgc ggatggcgtc atcaccaaca aaaagttgat
     6601 cgcgttcctg tcgtacttcc catgcaaaac ggccatggct ggtgtcatat ttcaacaaat
     6661 gcgccatgcc cgcagcatcc gccagttcgt tgattgccac cacggtaatt tccgcccggc
     6721 gtccggattc atacaaagca cgaaccacat tacgcccgat gcgaccgaag ccatttatcg
     6781 ctacgcgtac ggtcatagat ctcctgcaag gttttccctg agcaaatttg ccagacagag
     6841 taatccagca aatcgtccgg ggaaacctta cctgtcgcaa actgcgactg attggttaat
     6901 tgtcgaacat ttaatcgact gaaacgcttc agctaggata agcgaaacgt ggaataaaag
     6961 gaatgattgt ccagtcaaag aaaacaatta tctgacttac gtcacgtttt cagccccgcc
     7021 cctgtggaat ttattccact ttgcagaatc acgaataagc gtagcaaaat cttttcgttg
     7081 tgccgtcaca aaccctgact tatatcagaa tatgactgat gcagcgtcga tattatccgc
     7141 aatacgtccg agtttcggag tactccaatg atgacattgc cctacccata ttcttccccc
     7201 gcacgctttt atgcactgcg cctcctcccc gggcaggaag ttttttccca gctacacgct
     7261 tttgcacagc agcatcaact tcatgccgcc tggattgcag gctgtaccgg tagcctgact
     7321 gacgtcgcct tgcgctatgc cgggcaagaa ggcaccacgc tccttaacgg tacattcgaa
     7381 gtgatctcac taaacggcac gctggaacag agtggtgagc atcttcatct ctgcgtttcc
     7441 gatccacacg gcacgatgct tggcggtcat atgatgcccg gctgtaccgt gcggaccacg
     7501 cttgaactgg tgatcggtag ccttgaggaa ctggcgttta gccgccaacc ttgcgcgctt
     7561 tctggttacg acgagttgca tatttccccc gttaaataac tactttcaaa gaggttttct
     7621 tatggcgcgt agccactttt ccagtcaggc actggtgttg atcgttatat ctatcgccat
     7681 caatatgatc ggcgggcaac tggccagcat ggtcaaatta cctatatttc tcgactccat
     7741 cggcacactt attagcgcgg tactgcttgg tccggtgatc ggaatgctga ccggcttact
     7801 gaccaattta ctctggggat tgctgaccga cccgatcgcc gccgcatttg cacccgtcgc
     7861 gatggtcatc ggcctggtcg ccggttggct ggcgcgagcg ggttggtttc gcaccctgcc
     7921 aaaagtcgtt gtcagcggcg tgattattac gcttgcggta acggtcgtcg ctgtaccgct
     7981 gcgtactgcg ctgttcggcg gtgtcaccgg tagcggagcc gatttgttcg ttgcctggat
     8041 gcactcgatg gggcaaaacc tggtggagtc ggtggcgatt acagtgatag gcgcgaacct
     8101 ggtcgataaa attcttacag cggtgattgt ctggctttta ctgcgccagc tgccgattcg
     8161 caccacgcgc cattttccgg caatggctgc cgtgcgctaa atgcatccat ttacctcatt
     8221 aacactgtgg gcattggcgg cctgcaccac gcttatcctg cccgcgcaga ccattttgcc
     8281 gatctatagc gcggcgacct ttttttgtct tattgcgcta aaggccacgc ggcggcaggc
     8341 aaaatatgtc gcctggctga tgttctcgct gggagccggg ctgtggctgg tacacggtgg
     8401 ctggctaaca gaatggctaa gcggcacgcc gcgttcgccg gagcgttgga cgcatgctat
     8461 tacgctctgg ttgcggattc tggcgattgt ttcaacctca cagttgtgga tgcagtacgt
     8521 ccccgtgcaa tgttttatcc gcgctctgtt tgcttctcgc ctgccgccag gcgtcgctta
     8581 tctgtttgcc ggaccgttat tggtcgttga gcagttaaaa cggcaattag cgattattca
     8641 tgaagcgcag cgtgcccgcg gcgtgccgct ggatgagggc tggtatcagc gtttacgtgc
     8701 gatgcccgcg ttaattattc tgctaacaca caatgcgctt aacgatctgg ctgtccgtgg
     8761 tgcggcgctg gatatgcggg catttcggat ccacaaccga cgaacaacgt tgtgggcacc
     8821 agccgatagc accctacaac gcgtagcgcg ttacaccatg atcttgctga tgctaacaga
     8881 attcggagcg tggatatggt tacgttagaa cagttccgtt attgcccgac acactcaacg
     8941 catcctccgt tctgttatga ctttcactat gtcaaaccgg ggatggtcgc catctttggt
     9001 gacaacggca gtggcaaaag tacgctggca cagctgatgg cgggctggta tccggacttt
     9061 ctgccaggag agataacagg cacggggacg ctcctcggta cgcccattgg gcatctcccg
     9121 cttaatgaac aatccgccac catccagctc gtccagcaat cgccctattt gcagctctcc
     9181 ggctgtacct ttagcgtgga agaagaagtt gcctttgggc cagaaaatct gtgtcttgct
     9241 gaaacagaga ttatggcgcg gattgatgcc gccctgactc tgaccgagtg ccagccgttg
     9301 cgccatcgtc acccggcaac actctccggt ggagagacac aacgcgtggt catcgcctgc
     9361 gccatcgcga tgcagccaaa gctcttaatt cttgatgaag cctttagtcg cctgacgccg
     9421 caggccagcg aaatgctgtt gcagcgctta caacactggg cgcttgaacg tggttcgctg
     9481 attattcttt ttgaacgtca tcgcacacct ttcctgaact actgccagca agcgtggcaa
     9541 ttgcaaaacg gagcgctcca gccactatgt taacgttaaa tcagatatct tatcgctggc
     9601 ctagcgcagc aacggattgt ctttgcgaca tttctctgca actcaaacaa ggtgaatggt
     9661 tggcgctgac cggcgacaat ggcgcgggaa aatcgacgct gttacgagta atggcaggtc
     9721 ttcttacgcc cacagccggt acggttatgc tgcaacaaca ggcgatgaaa aacctgaaaa
     9781 accgccagcg cgcggcaaag gttggcgtac tgtttcagga agcggaaaac caactttttc
     9841 acagcaccgt cgctgacgag atcgcatttg gtttgaaact gcaaaaatgc ccggcggatg
     9901 aaatcactca acgtacccat gctgccctgc aatgttgtca gttagcagac actgccaact
     9961 cgcacccgtt ggatctgcat agtgcgcagc gacggatggt cgccgtcgcc tgcctggaag
    10021 cactctctcc gccactatta ctgctggacg agccgagtcg ggattttgac gaaaattggt
    10081 tgagcgtgtt tgaaagctgg ctggagaagt gccgccagcg aggcacgagc gtggtagcaa
    10141 tcagtcatga cgccgcattt acccggcgtc atttctctcg tgtggtgcga ctggaagatg
    10201 gtgtaatcag gaacgttaat ccaccagacg atattcaccc gtagcggtca tcattaaggt
    10261 caaatttgca ggcagactct cttccagcac ccggcgaaca ttcggcccgt cggtacggtc
    10321 ataaaaggct tctgcatctg ccagcgatac ccctcccgcc agcttgcgcc ccaccagacg
    10381 ctcccgcaac gcagtagctg gcgcgttgat aaagatcgag aaatcacaaa attgcgcaag
    10441 ctggcaccac ttctcgtcgt ccagcaacaa ccagtttcct tcgacgatga cgagtggtgc
    10501 ggtaacgtgc aacgcatctt caacaggatc atgcttttgt cgatcgtact gcggccacgt
    10561 acaatcccct tccaccacct ggcgcagatt ttccgccagt ttcgcaacgt caaatgtctc
    10621 tggtgcgcct ttgaaggggc gcaattgatg cgcgtccagc cagctattgt aatggtgaaa
    10681 accatccatc gggagcgtct gaatagcggg cagctccggg tcttgttgca cgagatattc
    10741 ccagaaggtg gttagggtag atttacccgt ccccggcgga gcgcaaagaa aaaccaccgt
    10801 acgccgctgc ggatttaccg tctgtaaggc cgccagcata cgcagcaacg gtttatgcac
    10861 gcgttctatc tcttcatcat ggtactgcgc ttgcacgttc agcccattca ccgttaattc
    10921 aattttcacg gcgttaattc ctttaaggtg gtcgttactg ccagcgtcag cgccgttttg
    10981 accattccgc taaaacgcgc c
//
LOCUS       DAZOZD010000137        10856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOZD010000137 DAZOZD010000000
VERSION     DAZOZD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.137"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            83..424
                     /locus_tag="ACHUPB_002896"
     CDS             83..424
                     /locus_tag="ACHUPB_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0759652.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="ACHUPB_002897"
     CDS             complement(415..1341)
                     /locus_tag="ACHUPB_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0759653.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            1431..2429
                     /locus_tag="ACHUPB_002898"
     CDS             1431..2429
                     /locus_tag="ACHUPB_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0759654.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="ACHUPB_002899"
     CDS             complement(2426..2644)
                     /locus_tag="ACHUPB_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0759655.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="ACHUPB_002900"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="ACHUPB_002900"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0759656.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="ACHUPB_002901"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="ACHUPB_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0759657.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="ACHUPB_002902"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="ACHUPB_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0759658.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="ACHUPB_002903"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="ACHUPB_002903"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0759659.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="ACHUPB_002904"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="ACHUPB_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0759660.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="ACHUPB_002905"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="ACHUPB_002905"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0759661.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..10828
                     /gene="crr"
                     /locus_tag="ACHUPB_002906"
     CDS             10319..10828
                     /gene="crr"
                     /locus_tag="ACHUPB_002906"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0759662.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2640 a         2798 c         2694 g         2724 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctgccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcaaaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aaacaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga gacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctgaat gtatcccttc taaatcctgt tgaaaatacg actcatatcg atatctatat
      421 ctgatacaac ggagcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgcaaaaa tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
     1861 caccattgct agttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cacttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
     5341 ctgcatcggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggatcggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaata agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttaac ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    10741 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    10801 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgccca
//
LOCUS       DAZOZD010000138        10832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOZD010000138 DAZOZD010000000
VERSION     DAZOZD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.138"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            46..807
                     /gene="adiY"
                     /locus_tag="ACHUPB_002907"
     CDS             46..807
                     /gene="adiY"
                     /locus_tag="ACHUPB_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0759663.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            944..2281
                     /gene="adiC"
                     /locus_tag="ACHUPB_002908"
     CDS             944..2281
                     /gene="adiC"
                     /locus_tag="ACHUPB_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0759664.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2385..4028
                     /gene="eptA"
                     /locus_tag="ACHUPB_002909"
     CDS             2385..4028
                     /gene="eptA"
                     /locus_tag="ACHUPB_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0759665.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            4025..4693
                     /gene="basR"
                     /locus_tag="ACHUPB_002910"
     CDS             4025..4693
                     /gene="basR"
                     /locus_tag="ACHUPB_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0759666.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4694..5794
                     /gene="pmrB"
                     /locus_tag="ACHUPB_002911"
     CDS             4694..5794
                     /gene="pmrB"
                     /locus_tag="ACHUPB_002911"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0759667.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(5770..5859)
                     /gene="pmrR"
                     /locus_tag="ACHUPB_002912"
     CDS             complement(5770..5859)
                     /gene="pmrR"
                     /locus_tag="ACHUPB_002912"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0759668.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5971..7473)
                     /gene="proP"
                     /locus_tag="ACHUPB_002913"
     CDS             complement(5971..7473)
                     /gene="proP"
                     /locus_tag="ACHUPB_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0759669.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7737..8603)
                     /locus_tag="ACHUPB_002914"
     CDS             complement(7737..8603)
                     /locus_tag="ACHUPB_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0759670.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(8600..10828)
                     /gene="crfC"
                     /locus_tag="ACHUPB_002915"
     CDS             complement(8600..10828)
                     /gene="crfC"
                     /locus_tag="ACHUPB_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0759671.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2708 a         2850 c         2670 g         2604 t
ORIGIN      
        1 gctttagcga gaactggtct tttattcgca ctcaggagta catgtatgag gatttgcagc
       61 gaccaacctt gtattgtttt attgactgaa aaagatgtct ggataagggt gaatgggaaa
      121 gaacctatta gccttaaagc taaccatatg gcgttattaa attgtgaaaa taatattatc
      181 gacgtctcct ctcttaacaa cactttggtt gctcatatta gtcacgacat catcaaagat
      241 tacctccggt ttctgaataa agatctctcg caaataccag tatggcaacg tagcgctacg
      301 cccatactca ccctgccatg cctgacgcca gacgtctttc gcgttgccgc gcaacacagc
      361 atgatgcccg cagaaactga gtcagaaaag gaacgaacac gcgcattatt attcacggtg
      421 ctatcccgtt ttctcgacag taaaaaattc ctttcattaa tgatgtatat gttacgtaat
      481 tgtgtaagtg acagcgttta tcaaattatt gaaagcgata ttcacaaaga ctggaatctt
      541 agtatggtag ccagttgttt atgtcttagc ccaagtctgt taaagaaaaa gctgaaaagc
      601 gaaaacacca gttatagcca aataatcacc acctgccgca tgcgttatgc cgtaaatgaa
      661 ttaatgatgg acggtaaaaa tatctctcag gtatcacagt cctgcggcta caacagtacg
      721 tcgtacttta tttctgtctt taaagacttc tacggtatga cgccgctgca ttatgttagt
      781 cagcacagag aacgaactgt cgcctgattt ttaaccttaa cgaagagcta tattaataac
      841 ggcatcagcg ataacccggt cgataataat tcaactatcg aatgcaggcg tatgatatga
      901 cgtaatttat tgtcacgaag ctcgccttcg caggagttta attatgtctt cggatgctga
      961 tgctcacaaa gtgggcttaa tccccgtcac cctgatggtg tcggggaata ttatggggtc
     1021 aggtgttttt ctgttacctg caaacctggc ctccactggc gggattgcca tttatggatg
     1081 gttggtgacg attatcggtg cgctggggct ctcgatggta tacgccaaaa tgtcgttcct
     1141 cgatccaagt cctggtggtt cttacgctta cgcccgccgc tgctttggcc cgtttctcgg
     1201 ttatcaaacc aacgtcctct actggctggc ctgctggatc ggcaatatcg ctatggtggt
     1261 cattggcgta ggatatttaa gttacttctt cccgattctg aaagatccat tggtattaac
     1321 catcacctgc gtcgtggtgc tgtggatctt cgtcctgctg aacattgtcg gtccgaaaat
     1381 gatcacccgt gtgcaggccg ttgccaccgt gctggcgctg atccccatcg tcgggattgc
     1441 cgtatttggc tggttctggt tccgtggtga aacctatatg gcggcatgga acgtcagcgg
     1501 cctgggcacc ttcggtgcga ttcaaagtac ccttaacgtt acgctgtggt cgttcatcgg
     1561 tgtggaaagt gcctccgttg ccgcaggtgt ggtgaaaaac ccgaaacgca atgtccctat
     1621 cgccaccatt ggtggggtat tgattgccgc cgtttgctat gtgctctcca ccaccgcgat
     1681 tatggggatg atccctaatg ccgcactgcg cgtttctgcc tcgccattcg gtgatgccgc
     1741 acggatggcg ttgggtgaca ccgctggggc cattgtttcc ttctgcgcag ctgcgggttg
     1801 cttaggttca ctgggcggct ggacgttgct ggcgggtcaa acggcgaaag ccgctgccga
     1861 tgacggactg ttcccaccga tttttgcccg tgtaaataaa gcgggtacgc cagtggcggg
     1921 gctgattatc gtcggtattt tgatgaccat cttccagctc agtagcattt cgccaaacgc
     1981 aaccaaagag ttcggtctgg tatcttccgt gtcggtcatc tttacactgg tgccatatct
     2041 ttacacctgt gcggcgttac tgctgctcgg ccacggtcac tttggtaaag cacgcccggc
     2101 atatctggct gttactacca ttgccttcct ctactgcatc tgggccgtgg tggggtccgg
     2161 ggcgaaagag gttatgtggt catttgtcac cctgatggtc atcaccgcca tgtatgcgct
     2221 gaattacaac cggctacata aaaacccgta tcccttagat gcaccaataa gcaaagatta
     2281 attcccctta atccagcaaa cataaaagcc aaccttaaga acttaaggtt ggcttaattt
     2341 tgctttgcga gcatatgcgc actttgttcg atggaaacac cgtgatgttg aagcgcctac
     2401 taaaaagccc ctctttgaat ttactcgcct ggctattgtt ggccgctttt tatatctcta
     2461 tctgcctgaa tattgccttt tttaaacagg tgttgcaggc gctgccgctg gattcgctgc
     2521 ataacgtact ggttttcttg tcgatgccgg tcgtcgcttt cagcgtgatt aatattgtcc
     2581 tgacactaag ctctttctta tggcttaatc gaccactggc ctgcctgttt attctggttg
     2641 gcgcggctgc acaatatttc ataatgactt acggcatcgt catcgaccgc tcgatgattg
     2701 ccaatattat tgataccact ccagcagaaa gttatgcgct gatgacaccg caaatgttat
     2761 taacgctggg attcagcggc gtgcttgctg cgctgattgc ctgctggcta aaaatcaaac
     2821 ctgccacctc gcgtctgcgc agtgttcttt tccgtggagc caatattctg gtttctgtac
     2881 tgctgatttt gctggtcgcc gcactgtttt ataaagacta cgcctcgttg ttccgcaata
     2941 acaaagagct ggtgaaatcc ttaagcccct ctaacagcat tgttgccagc tggtcatggt
     3001 actcccatca gcgactggca aatctaccgc tggtgcgaat tggtgaagac gcccaccgca
     3061 acccgttaat gcagaacgaa aaacgtaaaa atttgaccat cctgattgtc ggcgaaacct
     3121 cgcgggcgga gaacttctcc ctcaacggct acccgcgtga aactaacccg cggctggcga
     3181 aagataacgt ggtctatttc cctaataccg catcttgcgg cacggcaacg gcagtttcag
     3241 taccgtgcat gttctcggat atgccgcgtg agcattacaa agaagagctg gcacagcacc
     3301 aggaaggcgt gctggatatc attcagcaag cgggcatcaa cgtgctgtgg aatgacaacg
     3361 atggcggctg taaaggtgcc tgcgaccgcg tgcctcacca gaacgtcacc gcgctgaatc
     3421 tgcctggtca gtgcatcaac ggcgaatgct atgacgaagt gctgttccac gggctggaag
     3481 agtacatcaa taacctgcag ggtgatggcg tgattgtctt acacaccatc ggcagccacg
     3541 gtccgaccta ttacaaccgc tatccgccgc agttcaggaa atttacccca acctgcgaca
     3601 ccaatgagat ccagacctgt accaaagagc aactggtgaa cacttacgac aacacgctgg
     3661 tttacgtcga ctatattgtt gataaagcga ttaatctgct gaaagaacat caggataaat
     3721 ttaccaccag cctggtttat ctttctgacc acggtgaatc gttaggtgaa aatggcatct
     3781 atctgcacgg tctgccttat gccatcgccc cggatagcca aaaacaggtg ccgatgctgc
     3841 tgtggctgtc ggaggattat caaaaacggt atcaggttga ccagaactgc ctgcaaaaac
     3901 aggcgcaaac gcaacactat tcacaagaca atttattctc cacgctattg ggattaactg
     3961 gcgttgagac gaagtattac caggctgcgg atgatgttct gcaaacttgc aggagagtga
     4021 gtgaatgaaa attctgattg ttgaagacga tacgctgtta ttgcagggac tgattctggc
     4081 ggcgcaaacc gaaggctaca cgtgcgatgg cgtaacaacc gcgcggatgg cggaacaaag
     4141 ccttgaggcc ggtcattaca gcctggtggt actggattta gggttacccg acgaagatgg
     4201 actgcatttt ctcgcccgta tccggcagaa aaaatacacc ctgccggtac tgatcctcac
     4261 cgctcgcgat acgctgaccg acaaaatcgc cgggctggat gtcggtgccg atgactatct
     4321 ggtgaagcct tttgcgctgg aagagttaca cgcccgtatc cgcgccctgc tacgacgcca
     4381 taataatcag ggcgaaagtg agctgattgt tggcaatctg acgctgaata tgggtcgccg
     4441 tcaggtatgg atgggcggtg aagagttgat tctgacgccc aaagaatatg ctctgctgtc
     4501 acggttaatg ctcaaagcag gcagtccggt acatcgggaa attctctaca acgacatcta
     4561 taactgggac aatgaaccct cgaccaacac cctggaagtg catatccaca atctgcgcga
     4621 caaagtgggc aaagcccgta tccgcaccgt gcgcggcttt ggctatatgc tggtcgcgaa
     4681 tgaggaaaac taattgaatc tgatgcgttt tctgcgccga ccaatatcgc tgcgccaacg
     4741 gctgatattg accatcgggg ccattttgtt ggtgtttgag ctgatcagca tcttctggct
     4801 atggcatgaa agtaccgagc agattcagct gtttgagcag gcgctacgcg acaatcgcaa
     4861 caacgatcgc cacatcatgc gtgagatccg cgaggcggtc gccagcctga ttgtccccgg
     4921 cgtctttatg gtcagcctga cgctatttat ctgctatcag gcggtacgcc gcatcacccg
     4981 cccgctggcg gagctgcaaa aagagctgga agcgcgcacc gccgacaact taacgcccat
     5041 tgccattcac agcgccaccc tcgaaatcga agcggtggtt tcggcgttga acgatctggt
     5101 cagtcgcctg acgagcacgc tggataacga aaggttattt accgctgacg tcgcgcacga
     5161 actgcgaacg ccactggcgg gggtgcgttt gcatctggaa ctgctggcga aaacgcatca
     5221 cattgatgta gcaccgttag tggcacggct tgatcagatg atggagagcg tctcccagct
     5281 gctgcaactg gcgcgtgccg gacagtcatt ttcttccggt aattatcaac atgtaaaact
     5341 gctggaagat gtcattctcc cctcgtatga cgaactcagt accatgctcg accagcgaca
     5401 gcaaaccctg ctattgccag agagcgccgc cgacatcacc gttcagggtg atgcaaccct
     5461 gctgcggatg ttattgcgaa acctggtaga aaacgcccat cgttacagcc cacaaggcag
     5521 caacattatg attaagctgc aagaagatgg cggagcggtc atggccgttg aagatgaagg
     5581 accgggtatt gatgagagta aatgcgggga gttgagtaaa gcgtttgtac gtatggacag
     5641 ccgttatggc gggattggtc tggggttaag tattgtcagc cgcattacac agttgcatca
     5701 cgggcagttt ttcctgcaaa accggcaaga gacttccggc acgcgggcct gggtacggct
     5761 gaagaaagat cagtacgtag caaaccagat ataaagaaag ctgctgacca ccagcacact
     5821 gaacacggta gttaaacttt cataaacacg gtttttcatt gcactctcct ccgttaacct
     5881 ggaggagagt atgcgcgtca gagattaagt gaaccttaag agttcaattg gcctgataag
     5941 acagcgtcac atcaggccat ccgtttcagc ttattcatca attcgcggat gttgctgcac
     6001 caggcgggta cgtttcgcct gcaaatcggc aatctcgtgg tcaatatcat cgattttctg
     6061 ctcgatatta tcgtaatgct cgacgagaat ttccttcgct tcctgtatat ctgacgccgc
     6121 cggtgtcgca cctttcaacg gacgatttgc cgtctctttc atggttacgc cggtgattaa
     6181 accaatcacc gccactacca tcaggtaata ggcaggcatc atcagattct gcgagctttc
     6241 gaccagccag gccgccagtg ttggcgtcag accggcaacc agcaccgaaa tattaaatgc
     6301 cgccgccagc gcgctgtaac ggatatgcgt cgggaacatc gctggcaagg tagaagccat
     6361 aacgcccgta aagcagttaa ggatcaccgc cagcatcagt aacccggcaa aaatcaggcc
     6421 gatgacgtta ctgttaatca gaataaacgc cgggatcgcc aacacaaaca gggcaacact
     6481 accaagtagc acaaacggac gacggccaaa acggtcactc agcaagccca tcaccggctg
     6541 gacaaacagc ataccgatca taatggcgat aataatcagc accccgtggt cttcggagta
     6601 atgcaggtta tgcgacaaat aactcggcat ataggtcagc aacatgtagt aagtcacgtt
     6661 ggtggcaatt accagaccaa tacatgtcaa caggctgcgc cagtatttag tggcaatctc
     6721 tttaaacgag actttcgggc catcctgcaa accttcgcgg tcgccctgtt ccagtttatc
     6781 aacatgctgc tggaacgccg gagtttcttc cagcgcatgg cgcaggtaaa gcccgataat
     6841 ccctaacggc agagcaataa agaacggaat acgccagccc cagtcgagga agttcgcttc
     6901 gccgacaatg gtcgaaatta acaccaccac gcccgcaccc agcacaaacc cggcaataga
     6961 accgaagtcc agccagctgc ccataaagcc acgtttacgg tcaggggagt attccgcaac
     7021 aaatatcgac gccccggtat attcaccgcc gaccgagaaa ccttgtgcca tcttacagat
     7081 caacagcaga atcggtgccc aaataccaat cgtgtcgtag gacggtatta agccaataca
     7141 gaacgtactg atcgacataa tcacaatagt gatagcgagg atcttctggc gaccatattt
     7201 atcgcccaac ataccaaaga agagcccgcc aagcggtcga atcagaaagg gaacggagaa
     7261 agtggcaagt gcagcaacca tctgcacgct ggggtcagcc cccgggaaaa aaactttacc
     7321 taatgcgtaa gcaacaaaac cataaacacc aaaatcgaac cattccattg cattacccag
     7381 tgatgctgcg gtaatggctt tacgcagttt accgtcatca ataatggtga catcacgaag
     7441 ggtaatcggt tttacttttt tccttttcag catagctttc ctcgcagagt tggcccattt
     7501 ccgcaaacgc cagaaaaatc agcgcgacgg ccgcaaaagg ggagcgggaa ttgcaggatg
     7561 caatctctgt aacaagcgta gcgggtttgc ttacaccctc cggttaagga atgtacaatc
     7621 aaaccagttg acgcctgaat tgggcagtta atgacctttt taccctacca gtgtttaaat
     7681 ttgtgatcaa cttcacacat ggatttcgtt taggactcat tgatgtaact gattatttac
     7741 gccaaattta cagagaagat ttcctcgacc attgccccca ccaaacgctg cgctgaacag
     7801 agtcgtggca tccccagcgc gactgtttgc cagcgttggg tcactgacca gcaaacgggc
     7861 tgtttatctg ccgtggacca ctttcccagc caacttaaaa aatcagcatg ctcccctccc
     7921 gaagaggcaa accactgatg gtaatagtga cgcgtggtcg gtgccgcatt gatactctct
     7981 gccagatgtt gtgtcgcttt ctcgcgcaaa tcacccagta acaacaccac cgcttgcggg
     8041 cttcccagac ggcaggcatc gccaaatgca ccccagcgca actcctccag aacgataaaa
     8101 cagcgcccag gcaacgacca ggcgttatac gccccagcac gccagcgggt aaaaatttgc
     8161 tcgctctgat cacctgcctg caccagtagg cctcgttgcg ttaacgcctt ttccttccag
     8221 cgacaacggg ctgaaaacca ctcatacaag gcggcaattt gctgggggag ctccgggaaa
     8281 gcagagtgct ggcggtgatc cattttttgc agcagcgcca gatgctgcgc gatctgcgtc
     8341 agcgccagat gaccgggatc cagcatcgac gccagttttt ccaccattgt cagtcgcagg
     8401 ctaagcggcg catttaacat gccgccgttc gtccatgtct gaagctgcac acgcgaaaga
     8461 agttcctgca tcagtcgttc acgaaactgc tgctgatggg cttgtggaag gcgggcatca
     8521 tcgccctgcg ccagatccac taaaaagcgg ggataaaccg actccagcac gcgaccgggg
     8581 ccgtccagta acgtcttggt caatatcgtt ctgccgtgaa aagtgtttga atatcatcgc
     8641 gtaacagctg ggcgtcggtg taaatccagc cgtgagtcat cacagtctgc tgcaattgct
     8701 gctgcatcag cctgaccacc gattcatttt gttgacgcag agccaggctt tcgcgtaaac
     8761 gcgtctgtaa ttccgtcaaa catgaagcga actcagcgca aaaagtattc atgcctgccg
     8821 taacagattc atcgacctgc tctgccagaa ctttagccat ttgttggcaa taaagatcga
     8881 cttctgcgct taaagctcgt tgcaacacac tgtaatcaac cgtctctgtc ggggatttct
     8941 catttccccg tccccagtcg ggctgattca accagcgcga aaaagtctca cgcacaacgc
     9001 ctaaacgcgt gctctgctcg tccgttgcat cctggcgcga aatgacttca ctgaacagct
     9061 ggcgagtgtt gacgtgggga actactccgt gaaaaacagg aaaatgaacc ccaggacgaa
     9121 accctgactc gctcaattcc attttgactt gttgctcaat gggccgaata acatcggtta
     9181 acacccggca aagggtggat tcaagctcgg caaaacgcag cgtaaagtcg cgactgatgg
     9241 tgttctgcgc cgtctgtaac agtgtctcac agcaggtacg aatctcgctt aacggctccg
     9301 aatcatcctg aaacaaggcg gctaactgcg cattcagcgc atcttgctgt tgacgcagaa
     9361 agtggttggc ggaggtcagg gccagctcga tttcatgttt aatctcgccg ctcacctgcg
     9421 cctgattgag ttgcaatagc tgcaaacttt cttcgacctg atggatattt tgccgcaatt
     9481 gttcacaagc gacgtttaac ccgtgcgcac gaaaatccag gtattcccgc gcctgctgcg
     9541 cgtaattcaa cagtttatgc gcagcagatc gcagagcata caacgaggcg ttagcgtaag
     9601 cggcatgaag caacgcctga attggctggg cgaacagcga atcttcccac aactgatcgg
     9661 cagcatgacg aatatgttcg aggtccgcca gatcggcatg acgccagcgc ctgccgagcg
     9721 cggcatgggc aaaatcttcc acccagcgtt gttgctctgg cgctggtaac ttaccgttgt
     9781 tggctaactc atggcgcgct cgatttgcca agtagcccca catcgacgac accggaaata
     9841 tctgctgtgg cgtaatacag cctttcatca gcgtcccgga aatcagtgcc cgcacctggt
     9901 cggcgtcgtc actgttacga tcctgttgat cgaacttatt gaccagcaca tacagtggca
     9961 ccgattgccc caccgccaaa atcgcctcac ggacctcttc atcggagatc gatttcagtt
    10021 gcgtataatc cagcaccgcc agtaccgccg aggcgcgtgc cagctgctgg ttaagcattt
    10081 tttgcagatg cggttgcccg gcttcatttg gcccgggggt atccagtaac gtcaactgac
    10141 cgggataact ctccagcccc gccagatgga caaactccac ttcaatcacg ggaatatgct
    10201 caatggcggc gtaagcagaa aaaggaaaat cgacgtccag cgccttcgcc agtcgcacta
    10261 aatcattcaa acttttcaga caatgaaaaa taggctgggc acccagataa tatttttcga
    10321 aagcgacgcc attttcgatc cgctgcataa gcgcacgcat atctttatct atttccagca
    10381 catcggtcag atgcttaata tcgcaatcac gcaggcgctg ttgtaattgt tgaattaaac
    10441 aatcgattgg cgcgacatgt gaaaaatgca gtaccggttc cttttgcccg ggcgtatggc
    10501 gaataagcgt cggcagcgca gtcattgggc gattacgatt aggcagaacc tccgtaccaa
    10561 caatggcatt aatggtggtt gatttccctg ctttcatagt accgacaatc gccagcacca
    10621 tttccagtcg ggaaatttta ctcaactcat tattcagcat cgcgtgacgt tcggcgatat
    10681 taggctgact ccagggtaaa gccagttgtg gcgcgtcgtc tccgggtaca gagagaggca
    10741 ttttttccag taactgcaac tgttggcgag aaagctgtaa caggcgttca gcctcctgac
    10801 ttaactcata cagggtctgt gtgtacatag aa
//
LOCUS       DAZOZD010000139        10777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOZD010000139 DAZOZD010000000
VERSION     DAZOZD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.139"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHUPB_002916"
     CDS             92..1132
                     /locus_tag="ACHUPB_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0759672.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUPB_002917"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUPB_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0759673.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUPB_002918"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUPB_002918"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0759674.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHUPB_002919"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHUPB_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0759675.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUPB_002920"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUPB_002920"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0759676.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUPB_002921"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUPB_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0759677.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="ACHUPB_002922"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="ACHUPB_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHUPB_002923"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHUPB_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0759678.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         2750 a         2994 c         2619 g         2414 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaag
//
LOCUS       DAZOZD010000140        10775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOZD010000140 DAZOZD010000000
VERSION     DAZOZD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.140"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            139..1521
                     /gene="dosC"
                     /locus_tag="ACHUPB_002924"
     CDS             139..1521
                     /gene="dosC"
                     /locus_tag="ACHUPB_002924"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0759679.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1546..3945
                     /gene="dosP"
                     /locus_tag="ACHUPB_002925"
                     /gene_synonym="pdeO"
     CDS             1546..3945
                     /gene="dosP"
                     /locus_tag="ACHUPB_002925"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR0759680.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4204..4785
                     /gene="ddpX"
                     /locus_tag="ACHUPB_002926"
     CDS             4204..4785
                     /gene="ddpX"
                     /locus_tag="ACHUPB_002926"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR0759681.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4799..6349
                     /locus_tag="ACHUPB_002927"
     CDS             4799..6349
                     /locus_tag="ACHUPB_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0759682.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6351..7373
                     /locus_tag="ACHUPB_002928"
     CDS             6351..7373
                     /locus_tag="ACHUPB_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0759683.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7370..8266
                     /gene="ddpC"
                     /locus_tag="ACHUPB_002929"
     CDS             7370..8266
                     /gene="ddpC"
                     /locus_tag="ACHUPB_002929"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0759684.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8263..9249
                     /locus_tag="ACHUPB_002930"
     CDS             8263..9249
                     /locus_tag="ACHUPB_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0759685.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            9278..10168
                     /locus_tag="ACHUPB_002931"
     CDS             9278..10168
                     /locus_tag="ACHUPB_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR0759686.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(10224..10655)
                     /gene="osmC"
                     /locus_tag="ACHUPB_002932"
     CDS             complement(10224..10655)
                     /gene="osmC"
                     /locus_tag="ACHUPB_002932"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0759687.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2631 a         2676 c         2782 g         2686 t
ORIGIN      
        1 cgtagattga aactgtccta agtcgcttat ttcttttcag tatatcttca tatttcagga
       61 gaatatggtg cagttagtca atctcgcacg ttgcgttttg attccgtctg atttcccagc
      121 cttataaggg tgtgaattat ggagatgtat tttaaaagaa tgaaagatga gtggaccgga
      181 cttgtcgaac aggcagatcc actcattcgt gctaaagccg cggaaattgc cgttgcgcat
      241 gctcattatc tgagtatcga gttttatcga attgtccgca tcgacccgca tgccgaagaa
      301 ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg cgatggaacg ctggattatt
      361 aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa tacaaatcca gcataccgtc
      421 gcggaagtgc atgcccgcat aggaattccg gtagaaattg tcgagatggg gtttcgggtg
      481 ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt attccgccgc agaaaaactt
      541 caggtctatc atttctcgat taacagtatt gatatcgcga tggaagtgat gacccgcgcg
      601 tttaccttta gtgacagtag tgcctcaaag gaagatgaaa attatcgtat cttctcgtta
      661 ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct caatactttc atgggaaata
      721 gatattatct ataaaatcct gctggattct gatttaggca gtagtttgcc attaagccag
      781 gctgattttg gcctgtggtt taaccataaa ggtcgacatt attttagtgg cattgccgaa
      841 gtaggccata tctcccgtct cattcaggat ttcgacggta ttttcaatca aaccatgcgt
      901 aacaccagga atttgaataa cagaagtctg cgggtgaaat ttttattaca gataagaaat
      961 accgtatcgc agattattac cttgctgcgt gaattgtttg aagaagtatc gcgccacgaa
     1021 gtcggtatgg atgtactaac gaaattactt aaccgccgtt tcctgccgac tatcttcaaa
     1081 cgcgaaattg cccatgccaa ccggaccggt acaccgctgt cagtgctgat tattgacgtt
     1141 gataaattca aagagatcaa cgatacgtgg ggccataaca ctggcgatga aattctgcgt
     1201 aaagtctctc aggcctttta tgacaacgtc cacagtagtg attatgtttt tcgctacggg
     1261 ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa acgaaacgtt acgtaccgca
     1321 gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag ccgcaaacgg cgaggatatt
     1381 gccctctcac tttccatcgg tgccgccatg tttaatggtc atcctgacta tgagcgcctc
     1441 attcaaatag ccgatgaagc tctgtatatc gccaaaagac gaggtagaaa ccgtgttgaa
     1501 ctctggaaag ccagtcttta gatgcgccag gatgcagagg taatcatgaa gctaaccgat
     1561 gcggaaaatg ccgccgatgg catttttttc cccgcccttg aacaaaatat gatgggcgcg
     1621 gtgttaatta acgaaaatga tgaagtgatg tttttcaacc ccgccgcaga gaagctctgg
     1681 gggtacaaac gtgaagaagt cattggcaat aacattgata tgctgattcc acgggatttg
     1741 cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag gtggtaaagc gcgcgtcgag
     1801 gggatgagtc gggagctgca gctggagaaa aaagacggca gtaaaatctg gacccgtttt
     1861 gcgctatcga aagtgagcgc cgaggggaaa gtttattacc tggcgctggt acgggatgcc
     1921 agcgtagaaa tggcgcaaaa agaacagacc cgacaattga ttattgccgt tgaccatctc
     1981 gaccgaccgg tgattgtcct cgatccggaa cgccatattg tgcagtgcaa tcgcgcattt
     2041 accgaaatgt ttggttactg cattagcgaa gccagcggta tgcagcccga tacactcctg
     2101 aacattcctg aattccctgc cgataaccgc attcgtttac aacagttgct atggaaaacc
     2161 gcccgcgatc aggacgaatt tctgctgttg acacgcaccg gtgaaaaaat ctggattaaa
     2221 gcctctatca gcccggttta tgacgtgctc gcgcatctgc agaacctggt aatgaccttc
     2281 tcggatatca ccgaagaacg gcagatccgc cagcttgaag gcaatattct cgccgccatg
     2341 tgcagcagcc cgccatttca tgaaatgggg gaaatcattt gtcgtaacat cgaatctgtt
     2401 ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg ggatgccgat acactgggcg
     2461 tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct ggtcagcgac cattcgtcag
     2521 cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct cgtcaggagc agaaaccagc
     2581 gcctttatcg aacgcgtggc agatatcagc cagcatatgg ccgcgctggc gctggaacag
     2641 gaaaaaagcc gtcagcatat tgaacaactc atccaatttg atccgatgac cggtctgcca
     2701 aatcgcaata acctgcacaa ttacctcgat gaccttgtcg acaaagccgt ctctccggtg
     2761 gtgtatctca tcggtgttga ccatattcag gatgtgattg atagccttgg ctatgcgtgg
     2821 gccgatcagg cattgctgga agtggtcaat cgctttcgtg aaaaactcaa accggatcag
     2881 tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga gcctggaaaa cgacgtcagt
     2941 aacattaccc aaatcgccga tgagctacgg aatgtggtca gcaagccgat aatgattgac
     3001 gataaaccat tcccgctaac cttgagtatt ggcatcagct acgacgtggg taaaaaccgc
     3061 gattacctgc tctccactgc tcacaatgca atggattata ttcgcaagaa tggcggtaac
     3121 ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa aagagcgttt ggttttaggc
     3181 gcagcgctga aagaagcgat tagcaataac caactgaaac tggtttacca gccgcaaatc
     3241 ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg ctcgctggca cgatccccag
     3301 catggtcatg tacccccttc acggtttatt cctctcgcag aagagattgg tgaaatcgaa
     3361 aatattgggc gctgggtcat cgcggaagct tgccgtcagt tagcagaatg gcgtagccag
     3421 aatattcata tcccggcgtt atccgtgaac ttgtcggcgc tgcactttcg cagtaatcag
     3481 ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta ttgacggcca ccagctgacg
     3541 gtagaaatca cggaaagcat gatgatggaa cacgataccg aaatctttaa gcgcattcag
     3601 atcctgcgcg atatgggcgt gggcttatcg gtagatgatt ttggcacggg cttttccgga
     3661 ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa ttgacaaaag ttttgtcgat
     3721 cgttgtctga cggaaaaacg catccttgcc ttacttgaag ccattaccag cattgggcaa
     3781 agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca aagagcaatt tgagatgcta
     3841 cgcaagatcc actgtcgcgt tattcaggga tatttctttt cccgccccct acccgccgag
     3901 gaaattccag gctggatgag cagcgtgtta ccgctgaaaa tctgacaaat tcctctcgcc
     3961 cgcactcgcg ggttttcatt taacgtgacg ctgtcactta aatactgtga tttcagctac
     4021 gattccggga gattccttcc ttaacttccc ctcttgcacc aaaactgcac tacgctgcac
     4081 aaatgttgat caaaagttaa aaaatataac tttaagccag cattctggcg cttatcccgg
     4141 catggcatga gatctgcata agcggaaagc gcagcaattt ttgtcttaat acaggaaccg
     4201 tttatgtcgg ataccaccga actggttgat ttagccgtga tcttccctga tctggagatc
     4261 gaattgaaat acgcctgcgc tgataacatc acaggtaaag ctatttacca gcaagcgcgt
     4321 tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa gtatcagtat cgcccagctg
     4381 tcaggcttac aactggtgat ttacgatgcg tatcgcccac aacaagcaca ggcgatgttg
     4441 tggcaagcct gcccagaccc gcaatatgtt gttgatgtga cggtcggttc taatcacagc
     4501 cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg ggaacatcct ggatatgggc
     4561 gcagggttcg atgaaatgca cgagcgctcc catgcctatc acccttccgt cccgcccgcc
     4621 gctcagcgca atcgcctgtt gctgaatgcg ataatgactg gtggcggttt tgtcggtatc
     4681 tccagcgaat ggtggcactt cgaattaccc caggcagcga gttaccctct gctcgctgat
     4741 caattctctt gttttatatc ccctggcaca cagcacgtca gttaatcagg aaactgtcat
     4801 gaagagatcg atatcgtttc gtcccacatt gctcgcgctc gtccttgcca caactttccc
     4861 ggttgcgcac gccgccgtac cgaaagatat gctggtgatt ggtaaggccg ccgatccaca
     4921 aaccctcgac ccggcggtaa caatagataa taacgactgg acagtgacct acccgtctta
     4981 tcagcggctg gttcagtaca aaacggacgg tgataaaggc tcaaccgacg ttgaaggcga
     5041 tctggcaagt agctggaaag cgtctgacga tcaaaaagag tggacgttca ccctgaagga
     5101 caatgctaaa tttgccgatg gcacacctgt cactgccgaa gcagtaaaac tctcttttga
     5161 gcggttacta aaaatcggcc aggggccagc agaagcattt cccaaagatt taaagattga
     5221 tactcccgac gaacatacgg tgaagtttac ccttagccaa ccattcgcac cgttcctcta
     5281 cacgctagcg aatgacggtg catccattat caatccggcg gtcttaaagg aacatgcagc
     5341 ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt tccggaccat ttatgctgaa
     5401 aagcgggcaa aaaggtcagc aattagttct ggtgccaaat ccgcattacc ccggcaataa
     5461 accgaacttt aagcgagtat cggtaaaaat tatcggtgaa agtgcctccc gtcgcctgca
     5521 gctctcccgt ggcgacattg acattgccga tgcgctgccg gtggatcaac tcaacgccct
     5581 gaagcaggaa aataaagtca atgtggcaga gtatccgtca ctgcgcgtta cctatctgta
     5641 tctgaataac agcaaagcgc ctcttaatca ggcggatctg cgtcgggcca tttcctggtc
     5701 taccgattat cagggcatgg ttaacggcat tctgagtggt aacggaaaac agatgcgcgg
     5761 cccgattccg gaaggcatgt ggggctacga tgcgacggca atgcaataca accatgacga
     5821 aacgaaagcc aaagccgaat gggataaagt gacgagcaaa cccaccagcc tgacgtttct
     5881 ctactccgat aacgatccga actgggagcc tattgctctg gcgacacaat ccagtctcaa
     5941 caagctgggc atcaatgtga agctggaaaa gctggcgaac gccaccatgc gcgacagagt
     6001 gggtaaaggt gattacgaca ttgcgattgg caactggagt ccggattttg ccgacccgta
     6061 tatgtttatg aattactggt ttgagtcaga caaaaaaggt ctgccgggta accgctcgtt
     6121 ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg cttgcgacca ccgaccagac
     6181 gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc attgatgacg ctgcttatgt
     6241 gtacctgttc cagaaaaact accaactggc gatgaacaaa gaggtgaaag gctttgtgtt
     6301 caatcccatg ctggaacagg tcttcaatat caataccatg agtaaataac gtgaccttct
     6361 ggagtatttt acgccaacgc tgctgggggc tggtgctcgt ggtggcgggc gtctgcgtga
     6421 ttacgtttat tatctcgcat ctgatccctg gcgatccggc gcggttactg gcgggtgacc
     6481 gcgccagcga tgctatcgtg gaaaatattc gccagcaact gggactggac cagccactgt
     6541 atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg tgacctggga acatccattc
     6601 gtaccgggcg tccggtgctg gaagagttgc gtatattttt cccggcgacg ctggaactgg
     6661 ctttttgtgc cctgctgctg gcactcctga ttggcatccc gctgggcata ctctctgcag
     6721 tctggcgaaa tcgctggctg gatcatctgg tgcgaataat ggccattacc ggaatctcca
     6781 cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt ttatggtcat ctgcaaattc
     6841 ttcccggcgg cggaaggctt gatgactggc tggatccacc aacgcacgtt accggctttt
     6901 atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt cttcaatgcg ttgcaacatc
     6961 tcatcttacc ggcattaacg ctggcgttcg ttcacctggg aattgtcgct cgccagatcc
     7021 gctcagcgat gctggaacaa ttgagtgaag actacattcg taccgcccgg gccagcggct
     7081 tgcccggctg gtatatcgtt ttatgttatg cgctacccaa tgcgttgatc ccatcgatta
     7141 ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc agtgctcacc gaaaccgttt
     7201 ttgcctggcc cggaatgggt gcatgggtag taacatcaat acaggcgctc gacttcccgg
     7261 cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt gctggtcaac ctggtggtgg
     7321 atttgctcta tttgtggatt gatccgcgta tcggacgtgg aggtggtgaa tgatgctaag
     7381 cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga tttaacggtg caaaactggt
     7441 atggatgctg aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg tattaatgct
     7501 attgatgatg attttttcac cgtggcttgc gacgcatgat cccaacgcca ttgatttaac
     7561 cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc accgatgaag tgggacgcga
     7621 tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat tagtcgtggt
     7681 tgccattgcg ggtatgattg gttcgctact cggatgtcta tccggtgtgc ttggcggacg
     7741 cgcagacgcc atcatcatgc gcatcatgga cattatgctg tcgattcctt cgttggtact
     7801 gacaatggca ctggctgccg ctctcgggcc gagtttattt aacgccatgc tggcgattgc
     7861 tattgtgcga attccctttt atgtgcgcct ggcgcggggg caagcattag ttgtacgcca
     7921 gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg cgttggcatc tgatcagctg
     7981 gcatatttta cgtaactccc tgccgccgct gatcgtgcag gcatcgctgg atatcggtag
     8041 cgcgatttta atggccgcca cgttgggatt tattggcctg ggtgctcaac aaccgagtgc
     8101 tgaatggggc gcgatggtag ctaatggtcg caactatgta ctcgatcagt ggtggtattg
     8161 cgcatttccg ggggcagcga ttttgcttac cgccgtcggg tttaatctct ttggtgatgg
     8221 tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca acccgttctg
     8281 gacattcaac agctgcattt gagtttcccc ggttttaacg gttatgttca cgtgctcaac
     8341 aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc tggtgggaga atccggctca
     8401 ggtaaatcag tcaccgcaat gctgattatg cgtctgctac cgacgggcag ttattgcgta
     8461 catcgggggc agatttcact gctgggagac gatgttctta acgctcggga gaaacagctt
     8521 cgtcagtggc gcggcgcacg agtggcgatg atctttcagg aaccgatgac cgccctcaat
     8581 ccgacacgtc gaataggtct tcagatgatg gacgtgatcc gccatcatca accaataagt
     8641 cgtcgggaag ccagagctaa agcgattacc ctgctggaag agatgcaaat cccggatgcc
     8701 gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca gcgggtaatg
     8761 attgcgctgg cattctcctg cgagccgcaa ttgattattg ccgacgaacc gactacggcg
     8821 ctggacgtca cggtacagtt gcaggtactg cgtttgctta aacataaagc tcgcgccagt
     8881 ggaactgcgg tactgttcat cagccatgat atggccgtgg tgtcgcaact gtgcgatagc
     8941 gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg tgacggcaga cgttatccat
     9001 catccccggc atccgtatac cattggtttg ctgcaatgcg caccagaaca tggagtacca
     9061 cgccagccat tacccgccat tccagggacg gtaccaaacc tcacccattt gcctgacggc
     9121 tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt gtgaaaacgt cccggcgctg
     9181 acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt atccgcagca ggaggtcata
     9241 agtatctgac acgttattaa cgttacgcga cgtccatatc aatttcccgg cccgtaaaag
     9301 ctggcttggt aaaactacgg aacatgttca tgcgattaat ggtattgatt tacagatccg
     9361 ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc ggcaaaagca ccctcgcaca
     9421 gcttttaatg ggtatgctgc aaccgagcca cgggcaatac atccgttcag gatcacaacg
     9481 cattatgcag atggtgtttc aggacccgct ctcttcgctt aatccgcgct tatcggtgtg
     9541 gcgcatcatc acagaaccgc tctggatagc taagcgtagt agtgaacaac agcggcgagc
     9601 gttggcaaag gagttggctg tgcaggtggg tattcgtccg gagtatctcg accgcctgcc
     9661 tcatgcgttc tccggcgggc agcggcaacg catcgccatt gccagagcac tctcttcgca
     9721 gcctgacgtg attgtgcttg atgagccaac ctcggcgctg gatatctccg tgcaggcgca
     9781 gatcctcaat ttactggtaa cgctacagga aaatcacggg ctgacttatg tgctgatttc
     9841 acacaatgtc tcggtgatac gtcatatgag cgatcgggtg gcggtgatgt atctcgggca
     9901 gattgtagaa ctgggcgacg cgcagcaggt gctgacggca cctgcacatc catacacccg
     9961 attattgctg gattccctcc ccgccattga taaaccgctg gaggaagaat gggcattatg
    10021 taaaacggat ctgccgggaa accgcacgtt gccacaaggc tgttttttct acgaacgttg
    10081 ccctctggca acccacggat gtgaagtccg gcaatcatta gcaataaggg aggacggacg
    10141 tgagctccgg tgctggcggg cgctgtagag atgctaacaa cgcatcaggc attcacgcgc
    10201 ctgacgcgtc atccggcaat gctttacgat ttcaactggt aatccagcgt aatttccgct
    10261 ttcagtacct gagaaaccgg gcatcctgct tttgctttct ggatgatgcc gtcaaaggta
    10321 gaggcatcaa tacccggcac cgcaacttca ctcttcagtg cgattttcgt aatcgcaaaa
    10381 tcggcatcca ctttatccag cgacacatcg gcggtggtat caatcgatgt tggcgtgaat
    10441 cccgcttccc ccagcattaa tgaaagcgcc attgagaaac atgcggcatg cgctgcgcca
    10501 atcagttctt cagggttggt tcctttttcg ccttcaaaac gcgtgttaaa tccatacggc
    10561 tgttggttca gcacgccact ctgggtggat actgttccct tcccgcgttt gatatcgcct
    10621 tcccagtgtg cctgaccttt cttatggatt gtcattgttg ctctcctgtg ggcttaaaaa
    10681 ggaaattata gactacgtga gaaacagcac gaggataagc aggatattcc gaataaaata
    10741 cggcgaaaat attacttctg cacttaaatt aaatt
//
LOCUS       DAZOZD010000141        10715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOZD010000141 DAZOZD010000000
VERSION     DAZOZD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.141"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="ACHUPB_002933"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="ACHUPB_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="ACHUPB_002934"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="ACHUPB_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0759688.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="ACHUPB_002935"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="ACHUPB_002935"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0759689.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="ACHUPB_002936"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="ACHUPB_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /gene="ybjH"
                     /locus_tag="ACHUPB_002937"
     CDS             complement(4104..4388)
                     /gene="ybjH"
                     /locus_tag="ACHUPB_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0759690.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="ACHUPB_002938"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="ACHUPB_002938"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0759691.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="ACHUPB_002939"
     CDS             complement(5289..6497)
                     /locus_tag="ACHUPB_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0759692.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="ACHUPB_002940"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="ACHUPB_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0759693.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="ACHUPB_002941"
     CDS             complement(7292..8977)
                     /locus_tag="ACHUPB_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0759694.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="ACHUPB_002942"
     CDS             9247..9624
                     /locus_tag="ACHUPB_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0759695.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="ACHUPB_002943"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="ACHUPB_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0759696.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="ACHUPB_002944"
     CDS             10071..10358
                     /locus_tag="ACHUPB_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0759697.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..>10715
                     /gene="nfsA"
                     /locus_tag="ACHUPB_002945"
     CDS             10342..>10715
                     /gene="nfsA"
                     /locus_tag="ACHUPB_002945"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0759698.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2649 c         2713 g         2588 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttggg
//
LOCUS       DAZOZD010000142        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOZD010000142 DAZOZD010000000
VERSION     DAZOZD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.142"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUPB_002946"
     CDS             complement(<1..784)
                     /locus_tag="ACHUPB_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0759699.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUPB_002947"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUPB_002947"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0759700.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUPB_002948"
     CDS             1860..2747
                     /locus_tag="ACHUPB_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0759701.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUPB_002949"
     CDS             2780..3790
                     /locus_tag="ACHUPB_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR0759702.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUPB_002950"
     CDS             3860..5293
                     /locus_tag="ACHUPB_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0759703.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUPB_002951"
     CDS             5392..6741
                     /locus_tag="ACHUPB_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0759704.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUPB_002952"
     CDS             6731..7618
                     /locus_tag="ACHUPB_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0759705.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUPB_002953"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUPB_002953"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0759706.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUPB_002954"
     CDS             8352..9224
                     /locus_tag="ACHUPB_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0759707.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUPB_002955"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUPB_002955"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0759708.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUPB_002956"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUPB_002956"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0759709.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2532 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZOZD010000143        10504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOZD010000143 DAZOZD010000000
VERSION     DAZOZD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.143"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..697)
                     /locus_tag="ACHUPB_002957"
     CDS             complement(<1..697)
                     /locus_tag="ACHUPB_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0759710.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            953..1108
                     /gene="rseD"
                     /locus_tag="ACHUPB_002958"
     CDS             953..1108
                     /gene="rseD"
                     /locus_tag="ACHUPB_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0759711.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1105..1680
                     /gene="rpoE"
                     /locus_tag="ACHUPB_002959"
     CDS             1105..1680
                     /gene="rpoE"
                     /locus_tag="ACHUPB_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0759712.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1713..2363
                     /gene="rseA"
                     /locus_tag="ACHUPB_002960"
     CDS             1713..2363
                     /gene="rseA"
                     /locus_tag="ACHUPB_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0759713.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2363..3319
                     /gene="rseB"
                     /locus_tag="ACHUPB_002961"
     CDS             2363..3319
                     /gene="rseB"
                     /locus_tag="ACHUPB_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0759714.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3316..3795
                     /gene="rseC"
                     /locus_tag="ACHUPB_002962"
     CDS             3316..3795
                     /gene="rseC"
                     /locus_tag="ACHUPB_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0759715.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3993..5792
                     /gene="lepA"
                     /locus_tag="ACHUPB_002963"
     CDS             3993..5792
                     /gene="lepA"
                     /locus_tag="ACHUPB_002963"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0759716.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5808..6782
                     /gene="lepB"
                     /locus_tag="ACHUPB_002964"
     CDS             5808..6782
                     /gene="lepB"
                     /locus_tag="ACHUPB_002964"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0759717.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7055..7735
                     /gene="rnc"
                     /locus_tag="ACHUPB_002965"
     CDS             7055..7735
                     /gene="rnc"
                     /locus_tag="ACHUPB_002965"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0759718.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7732..8637
                     /gene="era"
                     /locus_tag="ACHUPB_002966"
     CDS             7732..8637
                     /gene="era"
                     /locus_tag="ACHUPB_002966"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0759719.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8649..9377
                     /gene="recO"
                     /locus_tag="ACHUPB_002967"
     CDS             8649..9377
                     /gene="recO"
                     /locus_tag="ACHUPB_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0759720.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9389..10120
                     /gene="pdxJ"
                     /locus_tag="ACHUPB_002968"
     CDS             9389..10120
                     /gene="pdxJ"
                     /locus_tag="ACHUPB_002968"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0759721.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10120..10500
                     /gene="acpS"
                     /locus_tag="ACHUPB_002969"
     CDS             10120..10500
                     /gene="acpS"
                     /locus_tag="ACHUPB_002969"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0759722.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
BASE COUNT         2616 a         2593 c         2827 g         2468 t
ORIGIN      
        1 agcccgcgcg ccacgccata gcaatgccat cgccagaaga aatatccgga ttggtggtgt
       61 actgatacac cttcgacgca ccgccggttg ccagtaccac tgcttttgcg tggcaggttt
      121 ccaccgtttc tttatttcgg ttccataccc acgcgcctac aacccgtcgc gtgcccggca
      181 ggccaatttt gtcagagaca atcagatcaa ctgcgttgct gcgctccagc acacgaatat
      241 tcggatggtt ctgcgccttg ctcaccagcg tggattgtac ttctctaccg gtggcgtcgg
      301 cggcatgaag aatacgacgg tgactatgtc caccttcacg ggtcagatgg tagctttctt
      361 cgccattcgg ttgaacgtgg gtatcaaaca aaaccccctg gtcaattaac cattgcacac
      421 aggaacgagc attgctggcg acaaattcaa ctgcatggcg atcgcaaata ccagccccgg
      481 caatcaatgt gtcttccaca tgcgagtcaa tgctgtcagt ttcatcaaac acggcggcaa
      541 taccgccctg ggcataaaat gttgaacctt ccgttaccgg gcctttactt agaacgatga
      601 cctgatgctg gtcagccagg cgtagcgcca gtgaaagtcc ggctgcgccg ctaccaataa
      661 tcaacacgtc acatgaatgt tcagggagag tattcatttt ctttgtttaa tttactaaac
      721 attgtttggt cagcatagca tcatgttgtg cgaataaaca cctgctattt taatatttgt
      781 tacagtggct aaacacgttg acgcagggcg gcgagaaaaa gagaagttac tggctggtgg
      841 aggattaggt ggtgaaataa aaaggccgtt gggttactct tcaggcagtt aaatgggcat
      901 ttctacacag ataatgcgat gttcagattc tgtagactta taatgataga taatgatccg
      961 tctacagcat gacaaacaaa aacagatgcg ttacggaact ttacaaaaac gagacactct
     1021 aaccctttgc ttgctcaaat tgcagctaat ggagtggcgt ttcgatagcg cgtggaaatt
     1081 tggtttgggg agactttacc tcggatgagc gagcagttaa cggaccaggt cctggttgaa
     1141 cgggtccaga agggagatca gaaagccttt aacttactgg tagtgcgcta tcagcataaa
     1201 gtggcgagtc tggtttcccg ctatgtgccg tcgggtgatg ttcccgatgt ggtacaagaa
     1261 gcttttatta aagcctatcg tgcgctggat tcgttccggg gagatagcgc tttttataca
     1321 tggctgtatc ggattgctgt aaatacagcg aaaaattacc tggttgctca ggggcgtcgt
     1381 ccaccttcca gtgatgtgga tgccattgaa gctgaaaact tcgaaagtgg cggcgcgttg
     1441 aaagaaattt cgaaccctga gaacttaatg ttgtcagaag aactgagaca gatagttttc
     1501 cgaactattg agtccctccc ggaagatttg cgcatggcaa taaccttgcg ggagctggat
     1561 ggcctgagct atgaagagat agccgctatc atggattgtc cggtaggtac ggtgcgttca
     1621 cgtatcttcc gagcgaggga agctattgat aacaaagttc aaccgcttat caggcgttga
     1681 cgatagcggg atactggata agggtattag gcatgcagaa agaacaactt tccgctttaa
     1741 tggatggcga aacgctggat agtgagctgc ttaacgaact ggctcataac ccagaaatgc
     1801 agaaaacctg ggaaagctat cacttaatcc gtgattcaat gcggggtgat actcccgagg
     1861 tgctccattt cgatatctct tcacgcgtga tggccgccat tgaagaagag ccagtacgtc
     1921 aaccggcgac attgatcccg gaagcccagc ctgcgccgca tcaatggcac aaaatgccat
     1981 tctggcagaa agtacgtccg tgggcggcac agcttaccca aatgggcgta gcagcatgcg
     2041 tatcgcttgc agttatcgtt ggcgtccagc actataatgg acaatctgaa acgtcccagc
     2101 agcccgaaac gccggtattt aatacactgc cgatgatggg taaagccagc ccggtaagcc
     2161 tgggagtacc ttctgaagcg accgcaaaca atggtcaaca gcagcaggta caggagcagc
     2221 gtcgtcgcat taatgcaatg ttgcaggatt acgaactgca acgccgactc cactctgaac
     2281 agcttcagtt tgagcaggcg caaacccagc aagccgctgt acaggtgcca ggacttcaaa
     2341 ctttaggaac gcaatcgcag taatgaagca actttggttt gccatgtcat tagtgacagg
     2401 tagcctgtta ttctctgcta acgcctcggc cactcccgcg tccggggcgt tattacagca
     2461 gatgaacctg gccagtcagt cactgaatta cgagctgtca ttcatcagca tcaataaaca
     2521 gggtgttgag tctctgcgtt atcgacatgc acgcctcgat aaccgtcctc ttgcacaatt
     2581 gttgcaaatg gatggcccgc gccgggaagt ggtacagcgc ggcaatgaaa tcagctattt
     2641 tgaaccggga cttgaaccgt tcacgcttaa tggcgattac attgttgatt ctctgccatc
     2701 gcttatctat accgatttca aacgcctttc tccttactac gactttatct ccgtcgggcg
     2761 cacgcgtatt gctgatcgtc tttgcgaagt cattcgcgtg gttgcccgag atggtacacg
     2821 ctacagctac atcgtgtgga tggacactga atcgaaatta ccgatgaggg ttgatctcct
     2881 tgatcgcgat ggtgaaacgc tggaacaatt tcgcgtgatt tcttttaacg tcaatcagga
     2941 tatcagcagc agtatgcaga cgctggcgaa ggcaaatttg ccgccgttgc tttctgttcc
     3001 tgtaggtgaa aaagctaaat tcagctggac gccaacctgg ttgccacagg gttttagcga
     3061 agtttccagt agtcgacgtc cgctaccgac gatggacaac atgcctatcg aatcacgtct
     3121 ctattccgac ggattattca gcttctcggt aaacgttaac cgcgctacgc catcgagcac
     3181 cgatcagatg ttgcgcaccg gacgcagaac cgtcagtaca agcgtacgtg ataacgccga
     3241 aatcaccatt gtcggtgaac tgccgccgca aacggcgaaa cgcattgccg agaatattaa
     3301 gttcggggca gcgcaatgat caaagagtgg gctaccgtcg tctcctggca aaacgggcag
     3361 gcgctggtca gttgtgatgt taaagcctca tgcagcagct gcgcttcacg cgccggttgc
     3421 ggtagccgcg tgttgaataa acttggcccg caaaccacgc ataccattgt cgtaccctgt
     3481 gatgaaccgt tagtgccggg gcaaaaagtg gaattgggga tcgccgaagg cagcctgctt
     3541 agctccgcat tactggttta tatgtcgccg ctggtgggat tattccttat cgcttcgcta
     3601 tttcagctac tctttgcttc tgacgttgca gcattatgtg gtgcgatcct tggcggtatt
     3661 ggcgggtttc tgattgcgcg cggttactcg cgtaagtttg ctgcccgggc agaatggcaa
     3721 ccgatcatat taagcgtggc attgccgcct gaccttgtgc gatttgaaac atcttcggaa
     3781 gacgcgagcc agtgatttca ctggctttgt tgcattttgc ataacaaatg cgaggacgtt
     3841 tccagatacc acaatccata cggtatgaac atttcctcgc ctcaatgttg tagtgtagaa
     3901 tgcggcgttt ctattaatac agacgttaag ctcagaacag cgacttctaa aagcctggtt
     3961 aaccgggcat taaggcacaa taatcatact ttatgaagaa tatacgtaac ttttcgatca
     4021 tagctcacat tgaccacggt aaatcgacgc tgtctgaccg tattatccag atctgcggtg
     4081 gcctgtctga ccgtgaaatg gaggcgcagg ttctcgattc catggatctt gagcgtgagc
     4141 gtggcattac catcaaagcg caaagcgtga cgctggacta caaagcgtct gacggcgaaa
     4201 cctatcagct taactttatc gacaccccgg gccacgtaga cttctcctat gaagtttccc
     4261 gttcgctggc tgcctgtgaa ggtgcattgc tggtggtcga cgccgggcag ggcgtggaag
     4321 cgcaaaccct ggcaaactgc tacaccgcca tggaaatgga tctcgaagtg gtgccagtac
     4381 tgaacaagat tgacctgccg gcagccgatc ctgaacgcgt ggcggaagaa attgaagata
     4441 tcgtcggcat cgacgccact gacgcggtgc gctgttcagc gaaaaccggc gttggcgtgc
     4501 aggacgttct cgaacgtctg gtgcgcgaca ttccgccgcc ggaaggcgat ccggaaggct
     4561 cgttgcaggc actaattatc gactcatggt tcgacaacta cctgggcgtt gtttcactta
     4621 tccgtattaa aaacggcacc ctgcgtaagg gcgacaaagt gaaagtcatg agtaccgggc
     4681 agacctataa cgccgaccgt ctgggcatct tcacgccgaa acaggttgac cgcactgaac
     4741 tgaaatgtgg cgaagtaggc tggctggtat gtgcgattaa agatatccat ggcgctccag
     4801 tcggcgatac cttaacgctg gcgcgtaatc cggcagaaaa ggcgctgcct ggctttaaga
     4861 aagtcaaacc gcaggtatac gccggtctgt tcccggtaag ttccgacgac tatgaagcct
     4921 tccgtgacgc gctgggtaaa ctcagcctga atgatgcctc actgttctat gagccggaaa
     4981 gctccagcgc gctgggcttt ggtttccgct gcggcttcct tggcctgctg cacatggaga
     5041 tcatccagga acgtctggaa cgtgaatacg atctggatct gatcaccact gcgccgaccg
     5101 tagtgtatga agttgaaacc acgtcaaggg aagttatcta cgtcgacagc ccatccaagc
     5161 tgcctgcggt aaataacatt tacgaactgc gcgagccgat tgcagagtgt cacatgctgc
     5221 tgccgcaggc atatctcggc aacgttatta cgctgtgcgt agaaaaacgc ggcgtgcaga
     5281 ccaatatggt ttaccacggt aatcaggtgg cgctgacgta cgagatcccg atggcggaag
     5341 tggtgctcga cttcttcgat cgcctgaaat ctacctcgcg tggttatgcg tctctggatt
     5401 acaacttcaa acgcttccag gcgtccgaca tggtacgtgt agacgtatta atcaacggtg
     5461 aacgtgttga tgcgttggcg ctgatcaccc accgtgataa ttcgcaaaac cgcggtcgcg
     5521 agttggtgga gaagatgaaa gatctgatcc cacgccagca gtttgatatc gccattcagg
     5581 cagcgattgg tacgcacatt attgcgcgat ccaccgtgaa acagctgcgt aaaaacgtac
     5641 tggctaaatg ttatggcggc gatatcagcc gtaagaaaaa gctgctgcag aagcagaaag
     5701 aaggtaagaa acgcatgaag cagatcggta acgtcgagct gccgcaggaa gcgttcctcg
     5761 ccattctgca cgtcggcaaa gacaacaaat aacccttagg agttggcatg gcgaatatgt
     5821 ttgccctgat tctggtgatt gccacactgg tgacgggcat tttatggtgc gtggataaat
     5881 tctttttcgc acctaaacgg cgggaacgtc aggcagcggc gcaggcggct gccggtgact
     5941 cactggataa agcaacgttg aaaaaggttg caccgaagct tggctggctg gaaaccggag
     6001 cttctgtttt tccggtgctg gctatcgtat tgattgtacg ttcgtttatt tatgaaccgt
     6061 tccagatccc gtcaggttcg atgatgccga ctctgttaat cggtgatttt attctggtag
     6121 agaagtttgc ttatggcatt aaagatccta tctaccagaa aacgctgatc gaaaccggtc
     6181 atccgaaacg cggcgatatc gtggtcttta aatatccgga agatccaaag cttgattaca
     6241 tcaagcgcgc ggtgggttta ccgggcgata aagtcactta cgatccggtc tcaaaagagc
     6301 tgacgattca accgggatgc agttccggcc aggcgtgtga aaacgcgctg ccggtcacct
     6361 actcaaacgt ggaaccgagc gatttcgttc agaccttctc acgccgtaat ggtggggaag
     6421 cgaccagcgg attctttgaa gtgccgaaaa acgaaaccaa agaaaatgga attcgtcttt
     6481 ccgagcgtaa agagacactg ggtgatgtga cgcaccgaat tctgacagtg ccgattgcgc
     6541 aggaccaggt ggggatgtat taccagcagc cagggcaaca actggcaacc tggattgttc
     6601 cgccgggaca atacttcatg atgggcgaca accgcgacaa cagcgcggac agccgttact
     6661 ggggctttgt gcctgaagcg aatctggtcg gtcgggcaac ggctatctgg atgagtttcg
     6721 ataagcaaga aggcgaatgg ccgacaggtg tgcgcttaag tcgcattggc ggcatccatt
     6781 aatagcccat cttcgttcac gttgtcgccg ttatggcgac aacgtgaatt atttatgaga
     6841 taaatctccc gtggctaacg acatcccccg tcgttgtgta tagaatattc ccccgaagtt
     6901 taaggttggc acctccaggt tgccacggca cacgaaacag cgttggtccc catataccgg
     6961 taaactgaaa ctgcagcgaa gcagttagca gagccatgta tatcaggtct gtttcgtgtg
     7021 ctgaattgtt gacgcattta tttattggta tcgcatgaac cccatcgtaa ttaatcggct
     7081 tcaacggaag ctgggctaca cttttaatca tcaggaactg ttgcagcagg cattaactca
     7141 tcgtagtgcc agcagcaaac ataacgagcg tttagaattt ttaggcgact ctattctgag
     7201 ctacgttatc gccaatgcgc tttatcaccg tttccctcgt gtggatgaag gcgatatgag
     7261 ccggatgcgc gccacgctgg tccgtggcaa tacgctggcg gaactggcgc gcgaatttga
     7321 gttaggcgag tgcttacgtt tagggccagg tgaacttaaa agcggtggat ttcgtcgtga
     7381 gtcaattctc gccgataccg tcgaagcatt aattggtggc gtattcctcg acagtgatat
     7441 tcaaaccgtc gagaaattaa tcctcaactg gtatcaaact cgtttggacg aaattagccc
     7501 aggcgataaa caaaaagatc cgaaaacgcg cttgcaagaa tatttgcagg gtcgccatct
     7561 gccgctgccg acttatctgg tagtccaggt acgtggcgaa gcgcacgatc aggaatttac
     7621 tatccactgc caggtcagcg gcctgagtga accggtggtt ggcacaggtt caagccgtcg
     7681 taaggctgag caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg aatgagcatc
     7741 gataaaagtt actgcggatt tattgccatc gtcggacgtc cgaacgttgg caaatccaca
     7801 ttgttgaaca aactgctggg gcagaagatc tccatcacct cccgcaaagc gcaaacaact
     7861 cgtcaccgca ttgtggggat ccatactgaa ggcgcgtatc aggcgatcta cgttgatacc
     7921 cctggcctgc atatggaaga aaaacgcgct attaaccgcc tgatgaacaa agcggcgagc
     7981 agctctattg gcgacgttga gctggtgatt tttgtcgttg aaggcacccg ctggacgccg
     8041 gacgacgaaa tggtgctcaa caaactgcgc gatggcaaag cgccggtgat cctcgcggtg
     8101 aacaaagtgg ataacgtgca ggaaaaagcc gatctgctgc cgcacttaca gttcctggca
     8161 agtcagatga acttcctcga tatcgtgccg atctctgctg aaaccgggct gaatgtcgac
     8221 actatcgcag caatcgtgcg taagcatctg cctgaagcga cccatcactt cccggaagat
     8281 tacatcaccg atcgctcaca gcgttttatg gcgtctgaaa tcatccgcga aaaactgatg
     8341 cgtttcctcg gcgctgaact gccgtactcc gtgaccgtgg agatcgaacg tttcgtctct
     8401 aacgaacgcg gtggttatga catcaatggt ttgattctcg ttgagcgtga agggcagaag
     8461 aagatggtta ttggcaacaa aggggccaag atcaaaacca tcgggattga agcgcgtaaa
     8521 gacatgcagg aaatgttcga agcgcctgtc caccttgaac tgtgggtaaa agtgaaatcc
     8581 ggttgggccg acgacgaacg cgcactgcgc agtctcggtt acgttgacga tctttaagag
     8641 tcactccgat ggaaggctgg cagcgcgcat ttgtcctgca tagtcgcccg tggagcgaaa
     8701 ccagcctgat gctggacgtc ttcacggagg aatcgggtcg cgtgcgtctg gttgccaaag
     8761 gcgcacgctc taaacgctct actctgaaag gtgcattaca gcctttcacc cctctcttgc
     8821 tacgtttcgg cgggcgtggc gaagtcaaaa cgctgcgcag tgctgaagct gtctcgctgg
     8881 cgctgccatt aagcggtatc acgctttaca gcggtctgta catcaacgaa cttctctccc
     8941 gcgtactgga atacgagacg cgcttctctg aactcttttt cgattacttg cactgcattc
     9001 agtctcttgc aggtgccact ggtacgccag aacccgcgct gcgccgcttt gaactggcac
     9061 tgctcgggca tctgggctat ggcgtcaatt ttacccattg cgcgggtagc ggcgagccgg
     9121 tagatgacac catgacgtat cgttatcgcg aagaaaaagg gtttatcgca agcgtcgtta
     9181 tcgacaataa aacgttcacc ggaaggcagt taaaagcgtt aaacgcgcgg gaatttcctg
     9241 acgcagacac actacgcgcc gcgaaacgct ttacccgcat ggcgcttaag ccgtatcttg
     9301 gcggtaaacc attaaagagc agggaactgt tccggcagtt tatgcctaag cgaacggtga
     9361 aaacacatta tgaatgatga ggattgtcat ggctgaatta ctgttaggcg tcaacattga
     9421 ccatattgca acgctgcgta acgcacgcgg tactgcttac ccggatccgg tgcaggcggc
     9481 gtttattgcc gagcaggcgg gagcggacgg cattaccgtg catttacgtg aagatcgccg
     9541 ccacattact gaccgcgatg tgcgcatcct gcggcagacg ctggataccc gaatgaatct
     9601 ggagatggca gtgaccgaag agatgctggc gatcgccgtt gagacgaagc cacatttttg
     9661 ctgcctggta ccggaaaagc gtcaggaagt aacaaccgaa ggcggcctgg acgttgcagg
     9721 gcagcgtgaa aaaatgcgcg atgcctgcaa acgtctgaca gatgccggga ttcagatttc
     9781 tctctttatc gacgccgatg aagagcagat caaagctgcg gcagaggttg gcgcaccgtt
     9841 tatcgagatc cacaccggtt gctatgctga tgccaaaact gacgccgaac aggcgcaaga
     9901 gctggtgcgt atcgccaaag ccgcgacctt tgccgctagt ctcggtctga aagttaacgc
     9961 cggacacggt ctgacctatc acaacgtgaa agccattgcc gccatccctg agatgcatga
    10021 actgaatatc ggtcatgcca ttattggccg tgcagtgatg accggactga aagatgcggt
    10081 ggcagaaatg aagcgtctga tgctggaagc gcgtggctaa tggcaatatt aggtttaggc
    10141 acggatatcg tagagatcgc tcgcatagaa gcagtgatcg cccgatccgg tgagcgcctg
    10201 gcacgccgcg tattaagcga taacgaatgg gcaatctgga aaacgcacca ccagccggtg
    10261 cgttttctgg cgaagcgttt tgctgtgaaa gaagccgcag caaaagcgtt tggcaccggg
    10321 atccgcaatg gtctggcgtt taatcaattt gaagtattca atgatgagct cggcaaacca
    10381 cggctacggc tatggggtga ggcattaaaa ctggcggaaa agctcggcgt tgtaaatatg
    10441 catgtaacgc tggcagatga acgccactat gcttgtgcca cggtaattat tgaaagttaa
    10501 aata
//
LOCUS       DAZOZD010000144        10452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOZD010000144 DAZOZD010000000
VERSION     DAZOZD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.144"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            38..613
                     /gene="ssuE"
                     /locus_tag="ACHUPB_002970"
     CDS             38..613
                     /gene="ssuE"
                     /locus_tag="ACHUPB_002970"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0759723.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            606..1565
                     /gene="ssuA"
                     /locus_tag="ACHUPB_002971"
     CDS             606..1565
                     /gene="ssuA"
                     /locus_tag="ACHUPB_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0759724.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1562..2709
                     /gene="ssuD"
                     /locus_tag="ACHUPB_002972"
                     /pseudo
     CDS             1562..2709
                     /gene="ssuD"
                     /locus_tag="ACHUPB_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2721..3512
                     /gene="ssuC"
                     /locus_tag="ACHUPB_002973"
     CDS             2721..3512
                     /gene="ssuC"
                     /locus_tag="ACHUPB_002973"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0759725.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3509..4276
                     /gene="ssuB"
                     /locus_tag="ACHUPB_002974"
     CDS             3509..4276
                     /gene="ssuB"
                     /locus_tag="ACHUPB_002974"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0759726.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4483..7095)
                     /gene="pepN"
                     /locus_tag="ACHUPB_002975"
     CDS             complement(4483..7095)
                     /gene="pepN"
                     /locus_tag="ACHUPB_002975"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0759727.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7361..8563
                     /gene="pncB"
                     /locus_tag="ACHUPB_002976"
     CDS             7361..8563
                     /gene="pncB"
                     /locus_tag="ACHUPB_002976"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0759728.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8736..10136
                     /gene="asnS"
                     /locus_tag="ACHUPB_002977"
     CDS             8736..10136
                     /gene="asnS"
                     /locus_tag="ACHUPB_002977"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0759729.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2357 a         2689 c         2823 g         2583 t
ORIGIN      
        1 ttaacaaaaa aagaacaatt ggttataagg agagagtatg cgtgtcatca ccctggcggg
       61 tagtcctcgc tttccttctc gctccagctc cttgctggaa tatgcgcggg aaaaactaaa
      121 tggcctggat gtagaggttt atcactggaa tctgcaaaac ttcgccccgg aagatctgct
      181 ttacgctcgt ttcgatagtc cggcactcaa gaccttcacc gaacagctgc aacaggctga
      241 tggactgata gtcgctacac ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct
      301 gctcgacctg ctgccagagc gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg
      361 cggtacggtg gctcatctgc tggcggtcga ttatgccctt aagccagttt taagtgcgat
      421 gaaagcccag gagctcctgc acggcgtgtt tgccgatgac tcacaagtta ttgattacca
      481 tcacaaaccc cagttcacgc caaatctgca aacccgtctt gataccgcgc tagaaacttt
      541 ctggcaggca ttgcaccgcc gcgatgttca ggttcctgac cttctgtctc tgcgaggtaa
      601 tgtccatgcg taacatcatt aaactggcgc tggcgggatt gcttagcgtc tctacttttg
      661 cggttgctgc agaatcctcg cctgaagcgt tacgtatagg ctatcagaaa ggcagtattg
      721 gtatggtatt ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa tcaaaaatct
      781 cctgggtgga gttccccgcg ggtccgcaaa tgttggaagc gttaaacgtt ggcagtattg
      841 atctcggcag taccggggat attccgccaa tctttgccca ggctgccggg gctgatttga
      901 tatatgtggg cgtcgagcca ccgaagccca aagccgaagt gattctggtg gcagaaaaca
      961 gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt tgcctttcag aaaggttcca
     1021 gttcacacaa ccttttactg cgtgcactgc gtcaggccgg acttaagttt actgacatcc
     1081 agcccactta tctgacgcca gctgatgccc gcgccgcgtt ccagcaaggt aacgttgacg
     1141 cctgggctat ctgggatccc tactactccg ctgcgttatt acagggcggc gtgcgggtgc
     1201 tgaaagacgg caccgatctc aatcaaactg gatcgtttta tctggcagct cgcccctatg
     1261 cagaaaaaaa cggcgctttt attcagggcg tactggcaac ctttagtgag gccgatgcgt
     1321 taacccgcag ccagcgcgag caaagcatcg ctttactggc aaaaacgatg ggcttaccgg
     1381 caccggtgat tgcctcgtat ctggatcatc gtcctcccac caccatcaaa ccggtgagcg
     1441 ctgaagttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaac cgtctggtgt
     1501 cgaaaaaagt tgatattcgc cagcgcatct ggcagcccac tcacctggaa ggaaaacaat
     1561 tatgagtctg aatatgttct ggtttttacc gacccatggt gacgggcatt atcttggaac
     1621 ggaagaaggt tcacgcccgg ttgatcacgg ctatctgcaa caaattgcgc aagcggcgga
     1681 tcgtctgggc tataccggtg tgctgatccc aacggggcga tcgtgtgaag atgcgtggct
     1741 ggtggcggcg tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg ccctgcgtcc
     1801 cagcgtaacc tcacctaccg ttgccgctcg ccaggccgcc acgcttgacc gtctctcaaa
     1861 tggtcgtgcg ttgtttaatc tggtcacagg cagcgatcca caagagttgg caggcgacgg
     1921 cgtgttcctt gatcatagcg agcgctacga agcctcggcg gaatttaccc aggtctggcg
     1981 gggcgcttat tgctggggga aagcgtcgat ttcaacggta aacatattca tgtgcgggga
     2041 gcaaaactgc tcttcccgcc gattcaacag ccgtatcctc cgctttactt tggcggatcg
     2101 tcagatgtcg cccaggagct ggcggcagaa caagttgatc tctacctcac ctggggcgaa
     2161 ccgccagagc tggtaaaaga gaagatcgaa caagtgcggg cgaaagctgc cgcgcatgga
     2221 cgcaaaattc gtttcggtat tcgtctgcat gtgattgttc gtgaaactaa cgacgaagcg
     2281 tggcaggccg ccgagcggtt aatctcgcat cttgatgatg aaactatcgc caaagcacag
     2341 gccgcattcg cccggacgga ttccgtagga caacagcgaa tggcggcgct acataacggc
     2401 aagcgcgaca atctggagat cagccccaat ttatgggcgg gcgttggctt agtgcgcggc
     2461 ggtgccggaa cggcgctggt gggcgatggt cctacggtcg ccgcgcgaat caacgaatac
     2521 gccgcgcttg gcatcgacag ctttgtgctt tcgggctacc cgcatctgga agaagcgtat
     2581 cgggttggcg agttgctgtt cccacatctg gatgtcgcca ttccggaaat tccccagccg
     2641 cagccgctga atccgcaagg cgaagcggtg gcgaatgatt ttatcccccg taaagtcgcg
     2701 caaagctaag gaacacgatg atggcaacgc cagtgaagaa gtggttattg cgcgttgccc
     2761 cctggttttt accggtgggc atcgtggcgg tgtggcaact ggcctcctcg gttggctggc
     2821 tgtcgacgcg tattttgcct tcaccggaag gagtggtgac ggcgttctgg acgctctccg
     2881 ccagcggcga attgtggcag catctggcga tcagttcctg gcgggcgctg attggctttt
     2941 caattggcgg atcgctgggc ttgattctgg gactgattag cgggctgtca cgctggggag
     3001 aacggctgct ggatacctca attcagatgt tgcgcaacgt accgcatctg gcgctgatcc
     3061 cgctggtgat tttgtggttt ggcattgatg aatccgcaaa aatctttctg gtggcgctcg
     3121 gcacgctatt ccccatttat atcaacacct ggcatggcat ccgtaatatc gatcgcggtc
     3181 tggtggaaat ggcgcgtagc tatggattat ccggcatacc gctgtttatc catgtgatcc
     3241 tgcctggtgc cctgccctca attatggtcg gcgtgcgttt tgcgttgggc ctgatgtggc
     3301 tgacgctgat tgttgccgaa accatttctg ccaattcagg cattggttat ctggcgatga
     3361 atgcgcggga gtttttgcaa acggacgtgg tggtagtcgc tattattctt tacgccctgc
     3421 tcggcaaact tgctgacgtc agcgcgcagc tgttagagcg cctctggttg cgctggaacc
     3481 cggcttatca tttgaaggag gccacggtat gaatactgct cgtctgaacc agggcacacc
     3541 attgttgctc aatgcagtaa gcaaacatta cgcggaaaat atcgtcctga accaactgga
     3601 tttacatatt ccggcaggtc agtttgtggc ggtggtgggc cgcagcggtg gtggcaaaag
     3661 taccctgctg cgcctgctgg caggtctgga aacgccaacc gcaggcgatg tgttagcggg
     3721 caccacaccg ctggctgaga ttcaggaaga cacgcgaatg atgtttcagg atgcgcgtct
     3781 gctgccgtgg aaatcggtga ttgataacgt tgggttaggc cttaaaggcc agtggcgcga
     3841 tgccgcgcgt cgggcgttgg ctgcagtagg actggagaat cgcgccgggg aatggcctgc
     3901 cgcactttct ggcgggcaga aacagcgtgt ggcgctggca agagcgttga ttcatcgacc
     3961 gggtttattg ttgcttgatg aaccgctcgg ggcactggac gccttaacgc gactcgagat
     4021 gcaggatttg attgtgtcac tttggcagca gcacggcttt accgtactgt tggtgacgca
     4081 tgatgtgagc gaagcggtag caatggctga ccgggtgttg ttaattgaag agggaaaaat
     4141 tggtctggat ttgacagtgg atatcccccg tccgcgtcga ttagggtcgg tgcgattagc
     4201 ggagctggaa gcggaagtat tgcaacgggt aatgcggcgt gggcattctg aacaactgat
     4261 tcggcgtcat ggttaatatg ccggatgtgg cgcacaaaac cagcattttc aacggacttg
     4321 taggcctgat aagacacgtc aagcatcgca tcaggcagtt gggcacggct gccggatgcg
     4381 gcgtgaacgc cttatccagc ctacaaaacc agcaatttca acggagttgt aggcctgata
     4441 agacgcgtca agcgtcgcat caggcatttt ggctcaaaag tatcaagcca gtgctttggt
     4501 gatcttctcg tacagatcgc cagagagatt ttccagccct ttcaactgtt ccagcgccgc
     4561 gcgcattttc tcctgacgtt tggcatcata acgtttcagg cgaatcagcg gttcaatcag
     4621 acgtgaagct acctgcgggt tacggctgtt gaggtcggta agcatttcct ccaggaactg
     4681 gtaaccgctg ccatcttcgg catggaacgc tgccgggttg ctgcccgcaa acgcaccaat
     4741 caacgaacga atacggttcg ggttgctcat ggtaaatgag cgatgctgca acagaccacg
     4801 caccgtctcc agcacattcg ccgccgggct ggtggcttgc aggataaacc atttatccat
     4861 caccagaccg tcctgatgcc acttatcgtc gtactcctgc atcagcgcgt cacggcaagg
     4921 cagctgtgca gcaaccgccg cagaaagcgc tgccagcgca tcggtcatat tgtttgcttc
     4981 atggtattgc ttgctcacca gcacgtcagc cagatgcgtt tcaccaaaag caaggaagcg
     5041 caggcaggca tttcgcagag tgcgttttgc aatatcttca tgctcaacac ggtactcgct
     5101 ctggtaattc gcgttgtaaa tagccagcag ctcatccgcc agttcagtcg ccagagtacg
     5161 agtgagtgct tcgcgtactt cggcaatagc aatcgggtcg atgatatcga acagttcagc
     5221 catttcattg acagaaggca gcgtcaggat ttctgccgcc agcgccggat caatcttctc
     5281 atcgagcagc accgcgcgga aagcgtcagc cacatgtacc ggcagagaca gcggctgccc
     5341 ttgctgatga cgggcgacgt tcagcttgat gtaggttgcc agcaaacttt gcgccgcatc
     5401 ccaacgggag aaatcattac gcgcatgacg catcaggaag gtcagttgct gatcactcca
     5461 cttatattcc agtttcactg gcgcagagaa ttcgcacagc agcgcaggca ccggctggaa
     5521 gtagacatta tcaaaaacaa aggtctgttc cgcctgggtg acgttcagca cggaattcac
     5581 cggatgaccg cctttctgca acgggatcac tttgccttcg ttgtcataca attcgatggc
     5641 aaacggaata tgcagcggct gtttttctgc ctgatccggc gtggctggcg tgcgctggct
     5701 gatggtcagg gtgtactgct cagtttccgg attgtagtcg tctttgacgg tcacaatcgg
     5761 cgtaccggac tggctgtacc aacggcggaa atgggagaga tcaacattcg acgcatcttc
     5821 catcgcctgc acaaagtcgt cacaggtcgc tgcgctacca tcatgacgct cgaaatagag
     5881 ttgcatccct ttctggaagt tttcttcgcc cagcagggtg tggatcatac ggatcacttc
     5941 cgcgcccttc tcgtaaacgg tcagggtgta gaagttgttc atctcaatga ccatgtccgg
     6001 gcggattggg tgcgccatcg ggctggcgtc ttccgcaaac tgcaatccgc gcatggtgcg
     6061 cacattattg atgcggttaa ctgcgcggga accaaggtca gagctgaatt cctgatcgcg
     6121 gaagacggtt aaaccttctt tcaggctgag ctggaaccag tcgcggcagg tcactcggtt
     6181 accggtccag ttatggaaat attcatggcc gataacgcgt tcaatatcga ggtaatcttt
     6241 gtcggtggcg gtgtcggtgc gggccagcac atatttggag ttaaagatat tcagaccctt
     6301 attctccatt gcgcccatat tgaagaaatc caccgcgacg atcatataga tgtcgaggtc
     6361 atactccagg ccgaagcgtt cttcatccca tttcattgag tttttcagcg aggtcatcgc
     6421 ccacggcgcg cgatcaaggt tgccgcgatc gacgtacagc tccagcgcca cttcgcgacc
     6481 agaacgcgtg gtaaaggtgt cgcgcagtac atcaaagtcg cctgccacca gcgcaaacag
     6541 gtagcacggt ttcgggaacg ggtcctgcca ctgtacccaa tggcgtccgt tttccagttc
     6601 accttgcgca acgcggttac cgttggaaag caggaaggga tatttggttt tatcggcaat
     6661 aattttggtg gtaaaacgcg ccagcacgtc cgggcggtcg agataatacg taatatggcg
     6721 gaaaccttcg gcttcacact gggtgcaaag cgcatcgcct gactgataaa gcccttccag
     6781 cgcggtattc gccgccgggc ttatttcatt aacgatctta agcgtaaaat gctccggcaa
     6841 attactgatg accagtgcgc cctcttcttc tttccaggcg gtccacggct catcattaat
     6901 atgaacagaa accagtttga ggtcttcgcc gttgagacga agtggagcat ctgatgcacc
     6961 atgacggaca gcctggctga ccgcggtaac gaccgtcttt tgcgcgtcga ggtcaaaggt
     7021 caagtcaata tcagtaatct ggtaatctgg cgcacgataa tcgtgacggt atttggcttg
     7081 tggctgttga gtcataaata acctttagca tcttttatag agtctggtgt tcagtctatt
     7141 cctgttgcgt aaatcgcgct atgcagaatc ttcatctttt caggtacaaa cgcctttatt
     7201 gctacatttt tataacatac accgcgtaat gccatcgacc agaaaggtgg catatggtgt
     7261 gatcggggtt caataaattg cgaaacaagg tatactccag cagttcctga agatgtttat
     7321 tgtactaaac gctcctgtac gaggacgcta ctgcgcacct atgacacaat tcgcttctcc
     7381 tgttctgcac tcgttgctgg atacagatgc ttataagttg catatgcagc aagccgtgtt
     7441 tcatcactat tacgatgtgc atgtcgcggc ggagtttcgt tgccgaggtg acgatctgct
     7501 gggtatttat gccgatgcta ttcgtgaaca gattcaggcg atgcagcacc tgcgcctgca
     7561 ggatgatgaa tatcagtggc tctctgccct gcctttcttt aaggccgact atcttaactg
     7621 gttacgcgag ttccgcttta acccggaaca agtcaccgtg tccaacgata atggcaagct
     7681 ggatattcgc ttaagcggtc cgtggcgtga agtcatcctc tgggaagttc ctttgctggc
     7741 ggttatcagt gaaatggtac atcgctatcg ctcaccgcag gccgacgttg cgcaagccct
     7801 cgacacgctg gaaagcaaat tagtcgactt ctcggcgtta accgccggtc ttgatatgtc
     7861 gcgcttccat ctgatggatt ttggcacccg tcgccgtttt tctcgcgaag tacaagaaac
     7921 catcgttaag cgtctgcaac aggaatcctg gttcgtgggc accagcaact acgatctggc
     7981 gcgtcggctt tccctcacgc cgatgggaac acaggcacac gaatggttcc aggcacatca
     8041 gcaaatcagc ccggatctag ccaacagcca gcgagctgca cttgctgcct ggctggaaga
     8101 gtatcccgac caacttggca ttgcattaac cgactgcatc actatggatg ctttcctgcg
     8161 tgatttcggt gtcgagttcg ctagtcggta tcagggcctg cgtcatgact ctggcgaccc
     8221 ggttgaatgg ggtgaaaaag ccattgcaca ttatgaaaag ctgggaattg atccacagag
     8281 taaaacgctg gttttctctg acaatctgga tttacgcaaa gcggttgagc tataccgcca
     8341 cttctcttcc cgcgtgcaat taagttttgg tattgggact cgcctgacct gcgatatccc
     8401 ccaggtaaaa cccctgaata ttgtgattaa gttggtagag tgtaacggta aaccggtggc
     8461 gaaactttct gacagccctg gcaaaactat ctgccatgat aaagcgtttg ttcgggcgct
     8521 gcgcaaagcg ttcgaccttc cgcatattaa aaaagccagt taatatcatc agggagctaa
     8581 tcggctccct ttttttacct ttaattccga aatctttcgc tgcatttgcg aattctgctt
     8641 gtctgattgc agatgccagg taacataggt atcccccccc ccatttacgg gtggacaagt
     8701 gtttattttt tccgactatt aacagagaga atattatgag cgttgtgcct gtagccgacg
     8761 tactccaggg ccgcgtagcc gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc
     8821 gccgagattc aaaagctggc atctccttcc tcgccgttta tgacggttcc tgctttgatc
     8881 ctgtacaggc tgtcatcaat aattctctgc ccaattacaa tgaagacgtc ctgcgtctga
     8941 ccaccggctg ctcggtcatt gtgacgggta aagtcgtggc atcgccgggc caggggcaac
     9001 aatttgaaat tcaggccagc aaggttgaag ttgctggttg ggttgaagat ccagacactt
     9061 acccgatggc ggcaaaacgc cacagcattg agtatctgcg tgaagtcgct cacctgcgtc
     9121 cgcgcacaaa cctgattggt gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc
     9181 atcgcttctt taacgagcag ggattcttct gggtttcaac gccactgatt accgcatctg
     9241 ataccgaagg tgcaggcgaa atgttccgcg tttctacgct ggatctggaa aacctgccgc
     9301 gtaacgatca gggcaaagtg gatttcgaca aagacttctt tggtaaagag tctttcctga
     9361 ccgtatctgg ccagttgaac ggcgaaacct acgcttgcgc attatccaaa atttatacct
     9421 tcggcccgac tttccgtgct gaaaactcca acaccagccg tcacctggcg gaattctgga
     9481 tgctggagcc ggaagtggcg tttgctaacc tgaacgatat cgcgggtctg gctgaagcca
     9541 tgctgaaata tgtcttcaaa gcggttctcg aagaacgcgc agacgacatg aaattcttcg
     9601 ctgaacgcgt agataaagat gccgtttcac gtctggaacg cttcattgag gccgatttcg
     9661 cgcaggtgga ttacaccgac gcagtgacca ttctcgaaaa ctgcggcagg aagtttgaaa
     9721 acccggttta ctggggcgtc gatctctctt ctgagcatga gcgttatctg gcggaagaac
     9781 actttaaagc accggtagtg gttaaaaact atccgaaaga tattaaagcg ttctatatgc
     9841 gcctcaacga agacggtaaa accgttgcgg ctatggacgt tctggctccg ggcatcggtg
     9901 agatcattgg tggctcccag cgtgaagaac gtctggacgt tctggacgag cgtatgctgg
     9961 aaatgggcct gaataaagaa gattactggt ggtatcgcga tctgcgtcgc tacggtactg
    10021 ttccgcattc aggtttcggt cttggttttg aacgcctgat tgcttacgta actggcgtgc
    10081 aaaacgtgcg tgatgtgatt ccgttcccac gtactccgcg caacgccagc ttctgatttt
    10141 cttcctcaaa gagccagcgc ctgctggctc ttttttatcc tttctgtcaa gttatctgtt
    10201 tgttaagtca agcaatctat ttgcaacccc gccataacga tcatcctgtt acggaatatt
    10261 acattgcaac atttacgcac aaaaaataac ccgcattctt attgcggatt agttttttct
    10321 tagctgatag cacaattttc atactatttt ttggcattct ggatgtctga aagaggattt
    10381 tgtgccaggt cgataaagtt tccatcagaa acaaaatttc cgtttagtta atttaaatat
    10441 aagtaaatca ta
//
LOCUS       DAZOZD010000145        10420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOZD010000145 DAZOZD010000000
VERSION     DAZOZD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.145"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUPB_002978"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUPB_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0759730.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUPB_002979"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUPB_002979"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0759731.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUPB_002980"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUPB_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR0759732.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUPB_002981"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUPB_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0759733.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUPB_002982"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUPB_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUPB_002983"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUPB_002983"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0759734.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUPB_002984"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUPB_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0759735.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUPB_002985"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUPB_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0759736.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUPB_002986"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUPB_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0759737.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2514 c         2752 g         2605 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc
//
LOCUS       DAZOZD010000146        10323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOZD010000146 DAZOZD010000000
VERSION     DAZOZD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.146"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            148..1542
                     /gene="galP"
                     /locus_tag="ACHUPB_002987"
     CDS             148..1542
                     /gene="galP"
                     /locus_tag="ACHUPB_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0759738.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1619..2116
                     /locus_tag="ACHUPB_002988"
     CDS             1619..2116
                     /locus_tag="ACHUPB_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0759739.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2211..2918
                     /gene="endA"
                     /locus_tag="ACHUPB_002989"
     CDS             2211..2918
                     /gene="endA"
                     /locus_tag="ACHUPB_002989"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0759740.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2998..3729
                     /gene="rsmE"
                     /locus_tag="ACHUPB_002990"
     CDS             2998..3729
                     /gene="rsmE"
                     /locus_tag="ACHUPB_002990"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0759741.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3742..4692
                     /gene="gshB"
                     /locus_tag="ACHUPB_002991"
     CDS             3742..4692
                     /gene="gshB"
                     /locus_tag="ACHUPB_002991"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0759742.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4801..5364
                     /locus_tag="ACHUPB_002992"
     CDS             4801..5364
                     /locus_tag="ACHUPB_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0759743.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5364..5780
                     /gene="ruvX"
                     /locus_tag="ACHUPB_002993"
     CDS             5364..5780
                     /gene="ruvX"
                     /locus_tag="ACHUPB_002993"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0759744.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5955..6935)
                     /locus_tag="ACHUPB_002994"
     CDS             complement(5955..6935)
                     /locus_tag="ACHUPB_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0759745.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            6953..7657
                     /gene="yggS"
                     /locus_tag="ACHUPB_002995"
     CDS             6953..7657
                     /gene="yggS"
                     /locus_tag="ACHUPB_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0759746.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7675..8241
                     /gene="yggT"
                     /locus_tag="ACHUPB_002996"
     CDS             7675..8241
                     /gene="yggT"
                     /locus_tag="ACHUPB_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0759747.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8238..8528
                     /gene="yggU"
                     /locus_tag="ACHUPB_002997"
     CDS             8238..8528
                     /gene="yggU"
                     /locus_tag="ACHUPB_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0759748.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            8536..9129
                     /locus_tag="ACHUPB_002998"
     CDS             8536..9129
                     /locus_tag="ACHUPB_002998"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0759749.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9122..10258
                     /gene="hemW"
                     /locus_tag="ACHUPB_002999"
     CDS             9122..10258
                     /gene="hemW"
                     /locus_tag="ACHUPB_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0759750.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2418 a         2720 c         2736 g         2449 t
ORIGIN      
        1 ataactcaat tattttcatg cacttaaacc ataactaaga taaatgttag tgtaagcgat
       61 tacactgatg tgatttgctt cacatctttt tacgtcgtac tcacctatct taattcacaa
      121 taaaaaataa ccatattgga gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac
      181 aaggcaatga cgtttttcgt ctgcttcctt gccgctctgg cgggattact ctttggcctg
      241 gatatcggtg taattgctgg cgcactgccg tttattgcag atgaattcca gattacttcg
      301 cacacgcaag aatgggtcgt aagctccatg atgttcggtg cggcagtcgg tgcggtgggc
      361 agcggctggc tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg cgcaattttg
      421 tttgttgccg gttcgctgtt ctctgcggct gcgccaaacg ttgaagtact gattctttcc
      481 cgcgttctac tggggctggc ggtgggtgtg gcctcttata ccgcaccgct gtacctctct
      541 gaaattgcgc cggaaaaaat tcgtggcagt atgatctcga tgtatcagtt gatgatcact
      601 atcgggatcc tcggtgctta tctttctgat accgccttca gctacaccgg tgcatggcgc
      661 tggatgctgg gtgtgattat catcccggca attttgctgc tgattggtgt cttcttcctg
      721 ccagacagcc cacgttggtt tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg
      781 ctacgcctgc gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt
      841 ttgcaggtta aacagagtgg ctgggcgctg tttaaagaga acagcaactt ccgccgcgcg
      901 gtgttccttg gcgtactgtt gcaggtaatg cagcaattca ccgggatgaa cgtcatcatg
      961 tattacgcgc cgaaaatctt cgaactggcg ggttatacca acaccacaga gcaaatgtgg
     1021 gggacagtga ttgtcggcct gaccaacgta cttgccacct ttatcgcaat cggccttgtt
     1081 gaccgctggg gacgaaaacc aacgctaacg ctgggcttcc tggtgatggc tgctggcatg
     1141 ggcgtactcg gtacaatgat gcatatcggt atccactctc cgtcggcgca gtatttcgcc
     1201 atcgccatgc tgctgatgtt tattgtcggt tttgccatga gtgccggtcc gctgatttgg
     1261 gtactgtgct ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac ctgctccact
     1321 gccaccaact ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg
     1381 ctgggtaacg ccaacacctt ctgggtgtat gcggctctga acgtactgtt tatcctgctg
     1441 acattgtggc tggtaccgga aaccaaacac gtttcgctgg aacatattga acgtaacctg
     1501 atgaaaggtc gtaaactgcg cgaaatcggc gctcacgatt aatctcctca ggcttcctcc
     1561 cgtcgcggag gaagccgcct cttgcagtca tcttttcttc gctctatcct ctgccgctat
     1621 gaaaacatcc cgtctcccta tcgccatcca acaggccgtt atgcgcggcc tgcgggaaaa
     1681 actcgcccag gccaacctga agctagggcg taactacccg gagccaaaac tctcttacac
     1741 ccagcgcgga acctccgccg gaacagcctg gctggaaagc tatgaaatcc gcctcaatcc
     1801 cgttttgctg ttggaaaaca gtgaagcttt tattgaagaa gtggtaccgc acgaactggc
     1861 acatttgctg gtatggaaac atttcggccg cgtagcgcca catggcaaag agtggaagtg
     1921 gatgatggaa agcgtgctgg gtgttcccgc ccgtcgtacg catcagttcg aactgcaatc
     1981 cgtgcgtcgc aacaccttcc cctaccgctg caagtgccag gagcatcagc ttaccgtacg
     2041 ccgccataat cgcgtagttc gtggcgaggc cgtctatcgc tgtgttcact gcggtgaaca
     2101 gctggttgcg aaataaccat ctgaactatc aggaactttc ctgatctggc tgattgcata
     2161 ccaaaacaac tttcgctacg ttgctggctc gttttaacac ggagtaagtg atgtaccgtt
     2221 atttgtctat tgctgcggtg gtactgagcg cagcattttc cggcccggcg ttggccgaag
     2281 gtatcaatag tttttctcag gcgaaagccg cggcggtaaa agtccacgct gacgcgcccg
     2341 gtacgtttta ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc
     2401 aatcgtgcgg ctatcaggtg cgcaaaaatg aaaaccgagc cagccgcgta gagtgggaac
     2461 acgtcgttcc cgcctggcag ttcggtcacc agcgccagtg ctggcaggac ggtggacgta
     2521 aaaactgcgc taaagatccg gtctatcgca agatggaaag cgatatgtat aacctgcagc
     2581 cgtcagtcgg tgaggtgaat ggcgatcgcg gcaactttat gtacagccag tggaatggcg
     2641 gcgaaggcca gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa gctgccgaac
     2701 caccagcgcg tgcacgcggt gccattgcgc gcacctactt ctatatgcgc gaccaataca
     2761 acctgacact ctctcgccag caaacgcagc tgttcaacgc atggaacaag atgtatccgg
     2821 ttaccgactg ggagtgcgag cgcgatgaac gcatcgcgaa ggtgcaaggc aatcataacc
     2881 cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc tacactagcg ggattctttt
     2941 tgttaacccc taccccacgc gtacaaccgc gtggggagac gacgcggatt tttaactatg
     3001 cgtatccccc gcatttatca tcctgaacca ctgaccagcc attctcacat cgcgctttgc
     3061 gaagatgccg ccaaccatat cgggcgcgta ctgcgcatgg ggccggggca ggcgttgcaa
     3121 ttgtttgacg gtagcaacca ggtctttgac gccgaaatta ccagcgccag caaaaaaagc
     3181 gtggaagtga aggtgctgga aggcaagatc gacgatcgcg aatctccgct gcatattcac
     3241 ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta cgatccagaa atcgatcgaa
     3301 ctcggtgtaa gcctcattac gccacttttt tctgagcgct gcggcgttaa actggatagt
     3361 gaacgtctga acaagaagct ccagcagtgg cagaagattg caattgctgc ctgtgagcag
     3421 tgtggtcgta accgggtgcc ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca
     3481 gagcaggatg aaggactgaa actgaatctt cacccgcgcg ccagtaacag catcaatacg
     3541 ttgccgttac cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg tttatcggca
     3601 gatgaaattg ccatgactgc ccgctatcaa tttactgata tcctgttggg acctcgcgtt
     3661 ttgcgtacag agacaactgc gctcaccgcc attaccgcgc tacaagtacg atttggcgat
     3721 ttgggctaac ggagaagaat aatgatcaag ctcggcatcg tgatggaccc catcgcaaac
     3781 atcaacatca agaaagattc cagtttcgct atgttgctgg aagcacagcg tcgtggttac
     3841 gaacttcact atatggagat gggcgatctg tatctgatca atggcgaagc ccgcgcccat
     3901 acccgcacgc tgaacgtgaa gcagaactac gaagagtggt tttcgttcgt cggtgaacag
     3961 gatctgccgc tggccgatct cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc
     4021 gagtttatct acgcgaccta tattctggaa cgtgccgaag agaaagggac gctgatcgtt
     4081 aacaagccgc agagcctgcg cgactgtaac gagaaactgt ttaccgcctg gttctctgac
     4141 ttaacgccag aaacgctggt tacgcgcaat aaagcgcagc taaaagcgtt ctgggagaaa
     4201 cacagcgaca tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg
     4261 aaagaaggcg atccaaacct cggcgtgatt gccgaaaccc tgactgagaa tggcactcgc
     4321 tactgcatgg cacaaaatta cctgccagcc attaaagatg gcgacaaacg cgtgctggtg
     4381 gtggatggcg agccggtacc gtactgcctg gcgcgtattc cgcagggggg cgaaacccgt
     4441 ggcaatctgg ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa
     4501 atcgcccgtc agatcgggcc gacgctgaaa gaaaaagggc tgatttttgt tggtctggat
     4561 atcatcggcg accgtctgac tgaaattaac gtcaccagcc caacctgtat tcgtgagatt
     4621 gaagcagagt ttccggtgtc gatcaccgga atgttaatgg atgccatcga agcacgttta
     4681 cagcagcagt aacccacctt agcgagaagg atctcgttga gactctgagt gacagcgccc
     4741 ttctttccac gcatactggg cgctgttgct tttttgaacc aggaaacaga acctctgaca
     4801 atgaatttac agcatcactt tcttattgcc atgcctgctc tccaggatcc gattttccgt
     4861 cgttccgtgg tctacatttg cgaacataat accaatggtg caatggggat catcgtcaac
     4921 aagccgctgg aaaatctcaa aattgaaggg attctggaaa agctgaagat cacgccggag
     4981 ccgcgtgatg aatcaatccg tctggataaa ccggttatgc tcggcggtcc gctggctgaa
     5041 gatcgcgggt ttattttgca tactccgccc tccaattttg cttccagcat tcgcatttca
     5101 gacaacacag tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa
     5161 ccgtctgacg tattggtggc tctaggttat gcctcctggg aaaaaggtca actggaacaa
     5221 gaaattctcg ataacgcgtg gctaacggcc ccggcggatc tgaatattct gttcaaaacg
     5281 ccgattgccg accgctggcg cgaggcggca aaactgattg gtgtggatat tctcaccatg
     5341 cctggtgtgg cagggcacgc ctgatgagtg gaaccttact cgccttcgac ttcggcacca
     5401 aaagcattgg cgtagcggtc ggccaacgca ttaccggcac tgctcgccct ttgcctgcaa
     5461 ttaaagcaca ggacggtacg ccggactgga acctcatcga acgtttactg aaagagtggc
     5521 agccggacga aatcatcgtc ggtttgccgc tgaatatgga cggcaccgag caaccattga
     5581 ctgccagagc gcgtaaattt gccaaccgta ttcatggtcg tttcggtgtt gaagtaaagc
     5641 tccatgacga acgtcttagc accgtggaag cccgttccgg tctgtttgaa cagggcggct
     5701 atcgggcgct caacaaaggc aaagtcgact ctgcctctgc ggttattatt ctcgaaagct
     5761 atttcgagca gggatattaa ggcgatttaa aagcctggcg gatagtcgtt accgccatct
     5821 tgccggatgc ggcgtgaacg cctatccggc ctacatgtgg cagcgattgt aggtctgata
     5881 agacgcgcaa acgtctcatc agacgtttat tgcctttgta ggcccgataa gcttgcacat
     5941 cgggcatggc aacgtcacaa acgcccttcc cccactcgct gctgaaaact ctgctgaaac
     6001 gttatcatcc ccacctgctg cccggtttga ataacatgcg gtaactggtg ggttttccct
     6061 tcgcgaatca aattccccac cgcgggagtg ttaatcaata attcaaacag cgccacgcgt
     6121 ccttcctgtt tatccacttc cagcttttgt gacaacacgg cccgtaaact acctgccagt
     6181 tgattacgta cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc
     6241 tgcgcggcac cacgcgtatg taatgttgcc agcaccaaat gcccggtttc tgccgccgtc
     6301 agtgccagac ggattgtctc gctgtcacgc agctctccga gcaaaatcac atcaggatct
     6361 tcccgcaatg cggcccgcaa tcccgatgcg aacgtcatac agtgcaaacc aatttcccgc
     6421 ttctggatca aacatcgctg gctggcatag atatattcaa caggatcttc cagcgtcaga
     6481 atatgcgcat cggcatgttg attgagatag ccaaccatcg ccgccagcgt ggtagatttg
     6541 ctgctccccg tcgcccccgt caccagaatc aggccattct cactcttgag taattccggc
     6601 aataccgttg gtgcgccaag ctgttcgagc tgcgggcagt gcgaaggtaa cagccgtaac
     6661 gccagcgaaa tgccatgccg ttgcgcaaat gcgctgccac gcaatcgctg gttttccgcc
     6721 agcgacacgg caaaatccag ctggccattt tccagcaata ttgcccgctg atcgtcatcc
     6781 agccactccc gcagtagctc ttcgacgtcc ggcgcctcaa acggcgcagc ttccattagc
     6841 ccgcgaatgc gccatcgtgc gggccaggcg ctgcacaggt gtagatccga gacgttatgc
     6901 tttacactaa gggccacaat ttcttccata ttcatactaa gatcctcgga aaatgaacga
     6961 tattgcgcat aacctggcac aggtccggga caaaatctca gcggctgcaa cgcgttgcgg
     7021 ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat
     7081 cgcagaagcc attgatgccg ggcagcgtca atttggtgaa aactacgttc aggaaggggt
     7141 agataaaatt cgccactttc aggaactggg cgtaacagga ttagaatggc attttattgg
     7201 cccgttgcag tctaataaaa gccgcctggt ggcagagcat ttcgactggt gtcataccat
     7261 cgaccgtttg cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac ttccccctct
     7321 taacgtactg attcaaatta atattagtga tgaaaacagt aagtccggga ttcaactggc
     7381 agaactggac gagctggcag cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct
     7441 gatggcaatc cctgcgcctg agtcagaata tgtaaggcag tttgaagttg cacgccaaat
     7501 ggctgtagca tttgccggac tgaaaacgcg ctacccgcat atcgacacgc tctctctggg
     7561 aatgtcggac gatatggaag ccgccattgc ggcaggtagc acgatggttc gtatcggcac
     7621 tgcaattttt ggtgcgcgtg attactctaa aaaataagga attaaaggaa cgccatgaat
     7681 acgttgactt tcctgctttc aacggtcatt gagctgtata ccatggtgct gttattacgc
     7741 atctggatgc agtgggctca ttgtgatttt tacaacccct tctcacagtt tgtagtgaag
     7801 gtaacgcagc caattatcgg gccactgcgc cgcgttattc cggcaatggg ccccattgac
     7861 agcgcctcgc tgctggttgc ctatattctc agttttatca aagccatcgt gctgtttaaa
     7921 gtggtgacct tcctgccaat catctggatt gctggtttac tgattctgct gaaaaccatc
     7981 ggcctgctga ttttctgggt cctgctggtg atggcgatta tgagctgggt aagccagggt
     8041 cgtagtccga ttgaatacgt gctgattcag ctggccgatc cgctgctgcg cccgattcgc
     8101 cgcctgctac cggcaatggg tgggattgat ttctcgccga tgatcctcgt tctgctgctg
     8161 tatgtcatca atatgggtgt cgcagaagta ttacaggcaa ccggaaacat gctgctgccg
     8221 gggctgtgga tggcgttatg agtgccgtaa cagttaatga tgacggtctg gttttacggc
     8281 tctatattca gccgaaagcc agccgtgatt ctattgtcgg tttacatggc gacgaagtta
     8341 aagtcgccat taccgcgccg ccggttgacg gccagggcaa cagtcatctg gtgaagtttc
     8401 tcggtaagca attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc
     8461 gccacaaaca aattaaaatc attaatccgc aacaaatccc gccagaaatc gtggcgttaa
     8521 ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt
     8581 gagctggcgt cgctgcttag cgacttcggt cttgatatcg tggcccaaac agacctcggc
     8641 gtagattccg ctgaagaaac cggcctgacc tttatcgaaa acgcgattct gaaagcgcgc
     8701 tatgcggcaa aagtgaccgg tttaccggca attgccgacg actctggtct ggcggtagat
     8761 gcgctgggtg gcgcgccggg tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat
     8821 caaaagaatc tgcaaaaact gttggaaaca ctgaaagacg taccggacga ccaacgtcag
     8881 gcgcgtttcc actgcgtgct ggtatatctg cgtcacgcgg aagatccgac tccgctggta
     8941 tgccacggca gctggccggg cgtgattact cgcgaaccag cgggcactgg tggctttggt
     9001 tatgatccaa tcttcttcgt accttccgaa gggaaaaccg ccgccgaact gacccgcgaa
     9061 gaaaagagcg ccatttccca ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt
     9121 aatggttaaa ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg
     9181 cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt
     9241 tcagcatctg ctttgcgatc tggacaatga tgtggcttac gctcagggcc gtgaagtaaa
     9301 gacaatcttt attggcggtg gtacgccgag cctgctttcc ggcccggcga tgcaaacgct
     9361 gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta ctatggaagc
     9421 gaaccctgga acggtagaag ccgatcgctt tgtcgattat cagcgtgctg gtgtgaaccg
     9481 catctctatt ggtgtgcaga gttttagcga agaaaagctg aaacgacttg ggcgtattca
     9541 tggcccgcaa gaagcgaaac gcgcggcaaa gctggcgagc gggctggggc tgcgtagctt
     9601 taacctcgat ttgatgcatg gactaccgga tcaatcactg gaagaggcgc ttggcgatct
     9661 acgccaggcc attgaactga atccgccgca tctttcctgg tatcaactga ccatcgaacc
     9721 caatacgctg tttggttcgc gaccaccggt gctgccggac gatgacgcgc tgtgggatat
     9781 tttcgaacag gggcatcagt tattaaccgc agcgggttat cagcaatatg aaacttccgc
     9841 ttacgccaaa cccggttatc agtgccagca caatctcaac tactggcgat ttggtgacta
     9901 catcggtatt ggctgcggcg cgcacggcaa agtcaccttc ccggatgggc gcattctgcg
     9961 taccactaaa acgcgtcatc ctcgtggttt tatgcaagga aggtatctgg aaagccagcg
    10021 tgatgtcgaa gccgcagata agccgtttga gttctttatg aatcgcttcc gtctgctgga
    10081 ggccgcgccg cgcgtggagt ttattgcgta taccgggctt tgcgaagatg tgattcgccc
    10141 acagttagac gaggcgattg cccagggtta tctcaccgaa tgtgcggatt actggcagat
    10201 aaccgagcac ggtaaattat tcttaaactc acttcttgaa ttattcctcg ctgagtaatc
    10261 attaatgcgc ctgcgggcgc attaatcact tctttccccc ccgttaatta acttcggcaa
    10321 aat
//
LOCUS       DAZOZD010000147        10086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZOZD010000147 DAZOZD010000000
VERSION     DAZOZD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.147"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHUPB_003000"
     CDS             3..308
                     /locus_tag="ACHUPB_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0759751.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHUPB_003001"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHUPB_003001"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0759752.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUPB_003002"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUPB_003002"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0759753.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUPB_003003"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUPB_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0759754.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUPB_003004"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUPB_003004"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0759755.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUPB_003005"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUPB_003005"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0759756.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUPB_003006"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUPB_003006"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0759757.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUPB_003007"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUPB_003007"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0759758.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUPB_003008"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUPB_003008"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0759759.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUPB_003009"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUPB_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0759760.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUPB_003010"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUPB_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0759761.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         2225 a         2825 c         2665 g         2371 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctat
//
LOCUS       DAZOZD010000148         9896 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOZD010000148 DAZOZD010000000
VERSION     DAZOZD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.148"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUPB_003011"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHUPB_003011"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0759762.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUPB_003012"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="ACHUPB_003012"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0759763.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="ACHUPB_003013"
     CDS             1905..2177
                     /locus_tag="ACHUPB_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0759764.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUPB_003014"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHUPB_003014"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0759765.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUPB_003015"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHUPB_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0759766.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUPB_003016"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHUPB_003016"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0759767.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="ACHUPB_003017"
     CDS             6190..6741
                     /locus_tag="ACHUPB_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0759768.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="ACHUPB_003018"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="ACHUPB_003018"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0759769.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="ACHUPB_003019"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="ACHUPB_003019"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0759770.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2472 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagtc
//
LOCUS       DAZOZD010000149         9878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZOZD010000149 DAZOZD010000000
VERSION     DAZOZD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.149"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="ACHUPB_003020"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="ACHUPB_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR0759771.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="ACHUPB_003021"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="ACHUPB_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR0759772.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="ACHUPB_003022"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="ACHUPB_003022"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0759773.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUPB_003023"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="ACHUPB_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="ACHUPB_003024"
     CDS             3565..3849
                     /locus_tag="ACHUPB_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0759774.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUPB_003025"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHUPB_003025"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0759775.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUPB_003026"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHUPB_003026"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0759776.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUPB_003027"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHUPB_003027"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0759777.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUPB_003028"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="ACHUPB_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0759778.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2390 a         2593 c         2503 g         2392 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatt
//
LOCUS       DAZOZD010000150         9804 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOZD010000150 DAZOZD010000000
VERSION     DAZOZD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.150"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(8..211)
                     /locus_tag="ACHUPB_003029"
     CDS             complement(8..211)
                     /locus_tag="ACHUPB_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759779.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            289..1383
                     /gene="ddlA"
                     /locus_tag="ACHUPB_003030"
     CDS             289..1383
                     /gene="ddlA"
                     /locus_tag="ACHUPB_003030"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0759780.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1407..1619)
                     /locus_tag="ACHUPB_003031"
     CDS             complement(1407..1619)
                     /locus_tag="ACHUPB_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0759781.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1879..2187
                     /locus_tag="ACHUPB_003032"
     CDS             1879..2187
                     /locus_tag="ACHUPB_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0759782.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2246..3340)
                     /gene="yaiW"
                     /locus_tag="ACHUPB_003033"
     CDS             complement(2246..3340)
                     /gene="yaiW"
                     /locus_tag="ACHUPB_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR0759783.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3353..4573)
                     /gene="sbmA"
                     /locus_tag="ACHUPB_003034"
     CDS             complement(3353..4573)
                     /gene="sbmA"
                     /locus_tag="ACHUPB_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0759784.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4925..6082
                     /gene="ampH"
                     /locus_tag="ACHUPB_003035"
     CDS             4925..6082
                     /gene="ampH"
                     /locus_tag="ACHUPB_003035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0759785.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6083..6706)
                     /gene="iprA"
                     /locus_tag="ACHUPB_003036"
     CDS             complement(6083..6706)
                     /gene="iprA"
                     /locus_tag="ACHUPB_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR0759786.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6794..9676)
                     /locus_tag="ACHUPB_003037"
     CDS             complement(6794..9676)
                     /locus_tag="ACHUPB_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0759787.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2527 a         2418 c         2348 g         2511 t
ORIGIN      
        1 ttaattatca acctttatta taaataaaag ttatgaaata tattctcttc gttaacttat
       61 aattaagaga aaaaaactca ccgcattgcc atttaagcca ggaataaatt agcttcccgt
      121 tcatcatttt tgtgatctcg cgcacatctt tgagataacg cactttcttc accagtacgt
      181 tgcacgcttt gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt
      241 tgtcgtggta atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg
      301 cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca
      361 aaaaacattg tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat
      421 aaacaagggc aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct
      481 gcccatattg cgttgcgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag
      541 catcagctta tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg
      601 attgtccacg gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat
      661 ttaccgtttg taggttctga tgttctggct tcagcagcct gtatggataa agatgtcacc
      721 aaacgtctgc tacgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgct
      781 aatcgtcaca acatcagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta
      841 aaaccggcta atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac
      901 gcaattgccg tcgatctggc gttcgagttc gatcataaag tgatcgttga gcaagggatc
      961 aaaggtcgtg agatcgaatg cgcagttctg ggcaacgaca atccgcaagc cagcacctgt
     1021 ggcgagatcg tactcaccag cgatttctat gcctacgaca ccaagtacat tgacgaagat
     1081 ggcgcgaaag tggtagttcc ggcagccatt gcaccagaga tcaacgataa gatccgggcg
     1141 attgccgttc aggcttatca gacgttggga tgcgcaggca tggcgcgtgt agacgtgttt
     1201 ttaaccccag agaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac
     1261 atcagtatgt atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca
     1321 cgtttgattg agctggcgct gaagcgtcac gctgcggata acgcattgaa agccacaatg
     1381 taatatttta acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga
     1441 cgaataatca accccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag
     1501 gtctgccaac cttttgcttc aaatcccagt aaccccacca cgccattaag aatgaatata
     1561 aggccaatgg cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg
     1621 gcagctccgt taacgaaaaa caacatcctt gcatattctg taacaaccgt ctgtggtgta
     1681 gacaacattt tcgtaattgt taataaattt aacttaaggt tgccattttg taactcacaa
     1741 cgaagattca cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca
     1801 atctaaatga cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga
     1861 tgacttccgg aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa
     1921 tatcgcaatg cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc
     1981 ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgt
     2041 ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc
     2101 attttcgggc tgatcccgtt tttagtaggc tgcctcattt ttgcagtggt ggcgctatgg
     2161 ctgcactggc gacatcgccg ccagtaagat ttacgttgcc agatacggcc taaaacgcct
     2221 tatctggcat gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta
     2281 cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa
     2341 tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa
     2401 ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc acctttctct aactgacggc
     2461 gaatttcgct gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt
     2521 tcccgggttc tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc
     2581 tggccttact gactgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa
     2641 aatcagcaaa gcggtatatc ggtgcgctat agctggcggg ataattcagt aaatggtaag
     2701 taccaaacca caacccgccg cggcgactga agacttcctg acgaactgta ccgtccattt
     2761 tccacggata ccctttggta tgctgttcgg caaaagcaat gctgacctgc atcggcccac
     2821 cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc cattggtacc atgctgatca
     2881 gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat
     2941 aacttttgcc gtttggcgat ttgattttta gcgcggtatg aaccagaaaa gcaggaatgt
     3001 gcatccgttc agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacgg
     3061 ccggatctgc ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt
     3121 ccagcgtcgg tgccagcccc tgactttcaa aggtgatggc gagatctttc gcccaggcat
     3181 cgcggtcttt cacgctcgca ggcatcttct gccgcacgac gctcgctaca tctatcgctt
     3241 tttcgccctt tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg
     3301 ccaacaatga cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca
     3361 aggtatgggt tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac
     3421 gtttgtagat agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag
     3481 caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc gtaatcgtac
     3541 cggcaacaat tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga
     3601 gaatgcgggc gatgttgaaa tacatatagt gaaaatagag gcgaaaatag tttttccgta
     3661 cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat
     3721 aaaccagctc tttacggtag gcggcctcta ctcgctggtt tttaaactcc agccccggca
     3781 gtttgatccc taccactgcc agccatccgg tccccatcag cgaccagacg atagcggcaa
     3841 tcaccagacc atacggaatg tgcccgataa tcggcagctc cggcacatgc gcggagagcg
     3901 ttaccagcac cggcaggaag gcgatcaacg tcatgatggc gttgataaaa ctgacgccca
     3961 tattctccag cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt gcggcacctt
     4021 cgatatgacg cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc
     4081 ggaacacata gtgactgaca aagaagttgt tcagtacact gatcaccacc gcgatcagcg
     4141 caatccccag aaagacgccc acttcgcggt aaaattgttc gatagtgact ttatgcggcg
     4201 aacttagcgc ggtttgaatc agatcataga acggtgcata ccaggcgttg acggcgaccc
     4261 cgacttccac caaaaaccag gtgacgaaga tgatcagtgc agtaccgagt attgaccagt
     4321 attgccaacg gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct acgcaaacaa
     4381 tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga
     4441 tctgcccgga agcgccggtg atacgcgcca cccagtctcc cccaccggct tgccagaaga
     4501 taacggcaat caatgcccaa acaaaggccg agagaaaaaa cgctcccggc tttgggaaaa
     4561 aagacttaaa catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta
     4621 accgcatgac cactacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag
     4681 gcgcttagtt cgccagcctg accataagaa ttgtttcgac ggatttcaca aaccaaagat
     4741 tgtttacata agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa
     4801 aaagatcgtg tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt
     4861 gcaatccatc aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc
     4921 accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt
     4981 ccatgcggcg cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc
     5041 cgatcatatt ttttacggca gcggtgcaac ggggatggcg ctggttgtta tcgacggtaa
     5101 ccagcgcgtc tttcgtagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct
     5161 ggattcggtc gtgcgtattg cttccctcac caaactaatg accagtgaaa tgctggtgaa
     5221 attgctcgac caggggaccg taaagttaaa cgatccgcta agtaaatatg ctccgccagg
     5281 cgcacgcgtg ccgacctaca atgggacgcc cattacccta gtgaatctgg caacccatac
     5341 cagcgccctg ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt ttgtctggcc
     5401 aacgcgcgag caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag
     5461 tcaggcggcg tactctaacc ttgcgttcga cctgctggca gatgcactgg cgaatgcgtc
     5521 cggcaagcct tatacccagt tgtttgaaga gcagattacc cgtccattgg ggatgaaaga
     5581 caccacctac accccctcac cggatcagtg ccgccgttta atggttgctg agcgcggtgc
     5641 cagtccgtgc aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgcctgg
     5701 cgatatgatg cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca
     5761 ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag tttacgaaag tgattggcat
     5821 ggatgtcccc ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg ccccgaaaga
     5881 gggtcgtccg gggattattc agaagacagg cggtggtggc ggtttcatta cctatatggc
     5941 gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc actcgttcgc cgttgacgcg
     6001 ctttaaaaat atgagcgatg gcatcaatga tctggtgacc gagctaagcg ggaataaacc
     6061 gttggttatc cccgcatcct gattaatact ctgaaggcaa acggttgata gcgaccagtt
     6121 tgcctttatt catttcgata tagccgcctt tacgcaaagc agcgagaact tctgcgacga
     6181 cagaacgcga tatgcgtgta cgttgatgga tatagttcat cacaccaata cgtgatcgca
     6241 attcttcatt ccagtcaatc attgataata atgttgcgcg gatttgttcg taggaattat
     6301 gcccaatgag ctgcacgtcg cgtaattcca gaattctatt ttgccaagct aaccagtaaa
     6361 aagcgtctcg ccagagttga ttttgttcaa taagcgtaat ggtttgtttg gctggtagat
     6421 gatatcccgt acaatttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac
     6481 catcagccag ccccataata taaggggcct gggtaatacc gataagtacg ttttcttctc
     6541 tacgcagaga gataactccc tctaaaataa cgaaagtatc ttcgttattg acatcactgg
     6601 aaaatataac ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt
     6661 tatcgagctt accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg
     6721 cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat taaattacca gtatttcttc
     6781 ggagtgaaga atattaccag gtatatttaa cacccacgtt cgcggaccag tcttgatcta
     6841 cgtcaccacc accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga
     6901 actgagtacc cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac
     6961 cgttcacatc gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag
     7021 tcagagcctg atcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac
     7081 gcatgctgtc gtaagactga ccgtcaactt tcatgtcgtt gctcagctgg tagtcatcac
     7141 cagactggaa cagaccagaa acgctgccgt aaggagtcac gtaaccagca tcacccagtt
     7201 tgaagtcgta accggctttc aagccgaagc cccaagcgtc ggagttggtg ctaccgtcaa
     7261 cgtaagtacc gttgctcatg gttgcagaca ggtcgttgtt gaagtgagag tagctcaagc
     7321 taccatcaac aaagacgttg ttcgcgaagt gagcagaaga gtagatgtag gcagtctggc
     7381 tgtcttgatc tacctgacca gaacggtcat tcatgtcacc tttagcgaag cctgcagccg
     7441 caccgacgat ccacttagcg ttgttaccgt caactttggt atcaacaccg accatgatgc
     7501 cgttaacatc ctgatcatag ttgatggtgc cgttgtcgcc gttgaagtta ccaccgaagt
     7561 agcttaccca tgcgccgccg ttatcagcca ggccatgacg agagttggtc agacgagtac
     7621 caacggtgtc ttgttccagg ttccagatat tggtgttcgc agacgggatg ctcagcgcca
     7681 tgttagcgta gtcggtcagc tccatctgtt gcagaacaac ggtgttaccg cgctgttcag
     7741 cctgataggt gtatgcaccc aggtcagctt tgttagcagc agagaaggtc gcgttggttt
     7801 tggtatcatc gatatagatg agctgcttac ctgcgtaatc agcaatcgca ccagcgccag
     7861 tggagttgtc gatacgaact ttgtagttac cggttgcagc tacaacgttg tctgcagagc
     7921 tgttgtcaac ttcgtcgcca ttgttaatgt cgttgttacc attaacggtc aagtgaccat
     7981 cagagttcat cgcaataaca ccataaccgt agttagcttt gctggtgtcg ttggtacgat
     8041 cgttgacgag gtctgcattc aggatgtaat cattactatg aatgtcaaac acacctgcgt
     8101 aaacattacc gttgttatct ttaacgttgc cactggtcag ttccagagta tcagttgcaa
     8161 atacgctgga gatgtcattc atgttggtgt ttacatccag cacaccaccg ttagtaatgg
     8221 tattggcata cagcggtgca tatgcccaag ctgaagcagt atcttcaccg aagttaacgg
     8281 ttgagaaggt atcaatggtc aggtgatcgg tgttaacttc accgttaact tccattgaag
     8341 aaccaccgtt cagagtgatg gtgtcagaag tcagagaaga atctgcactc acgttaacct
     8401 gagaaccgct gttgatcgcc agggtatcga tcagggaagt tttcgtggtt tcccaatcag
     8461 aaccgttgtt cagaacaaca tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac
     8521 gattccagcc atcttcagag taagaagcgt acgggttcag gctgtttggt gcaacgtcgt
     8581 agaaggcttc gtcagcaaca acaacgtcat aatcaatatt cgctgaacca acccatttgg
     8641 tgttgttcag agtcaggttc aggtgatcga cgttctgatc gtcagttgcc caaccgccat
     8701 gagtgtaagc agtggtagag ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc
     8761 aggtactgtc aaatacaacg tcaccaagca actgagagtt gttgaacgta acgttgttga
     8821 ccatacgata atcagaagtg tcatcatcga tgaaggctaa cgcaacatca tcagcaggag
     8881 atgcgccatt gccgtagtca cttggttcaa cagaattgcc gaaatgacca cgatctgatt
     8941 gttcgtcttc agtccatgaa ccagaagtta cagtagagtc attaacaacg atgttgttgt
     9001 cccactgatg accttcggta ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa
     9061 tacctgcaac atgggagttg ttgctgatgt tgatgttact ttcaacatcc agagtcacag
     9121 cggtaccaag accagcggtt accaaacgat cataatctgt cttagatgtt ttaccgtctg
     9181 catccagata agtatcggtg aagtagaagg cgtcaaaatc atcgtcaata gtagaattag
     9241 cgatgttcag agtgaagatg tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt
     9301 tggtccagac gtagtcgcca taaccaacct ggtaagatgt aaccatacca tgaacaattg
     9361 agttggagat gttcagtgta ttaccggtat ctgtgccata atcgtggttc agataataag
     9421 tagaaattac accatttaca gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg
     9481 cgtggtcata tacataacca ccgtaagtca gataatcgtg atcaatttta gtgctgtcac
     9541 cccaattgta gatggtggct gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat
     9601 tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct
     9661 ttttccagga gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta
     9721 tcaatgcata gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat
     9781 ggttcaatta ttctttcccc taag
//
LOCUS       DAZOZD010000151         9794 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOZD010000151 DAZOZD010000000
VERSION     DAZOZD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.151"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            466..2097
                     /gene="oppA"
                     /locus_tag="ACHUPB_003038"
     CDS             466..2097
                     /gene="oppA"
                     /locus_tag="ACHUPB_003038"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0759788.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2183..3103
                     /gene="oppB"
                     /locus_tag="ACHUPB_003039"
     CDS             2183..3103
                     /gene="oppB"
                     /locus_tag="ACHUPB_003039"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0759789.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3118..4026
                     /gene="oppC"
                     /locus_tag="ACHUPB_003040"
     CDS             3118..4026
                     /gene="oppC"
                     /locus_tag="ACHUPB_003040"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0759790.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4038..5051
                     /gene="oppD"
                     /locus_tag="ACHUPB_003041"
     CDS             4038..5051
                     /gene="oppD"
                     /locus_tag="ACHUPB_003041"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0759791.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5048..6052
                     /gene="oppF"
                     /locus_tag="ACHUPB_003042"
     CDS             5048..6052
                     /gene="oppF"
                     /locus_tag="ACHUPB_003042"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0759792.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6105..6434)
                     /locus_tag="ACHUPB_003043"
     CDS             complement(6105..6434)
                     /locus_tag="ACHUPB_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0759793.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6469..7929)
                     /gene="cls"
                     /locus_tag="ACHUPB_003044"
     CDS             complement(6469..7929)
                     /gene="cls"
                     /locus_tag="ACHUPB_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0759794.1"
                     /translation="MTTVYTLVSWLSILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8072..8245
                     /locus_tag="ACHUPB_003045"
     CDS             8072..8245
                     /locus_tag="ACHUPB_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0759795.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8300..9553)
                     /gene="kch"
                     /locus_tag="ACHUPB_003046"
     CDS             complement(8300..9553)
                     /gene="kch"
                     /locus_tag="ACHUPB_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0759796.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2568 a         2281 c         2430 g         2515 t
ORIGIN      
        1 ttattgtgca tttcactact ggaactgtaa tcagaaaaga tagacatgct tagccaatct
       61 ctatttgatt gaattgaaag atgtttgtta aggcatggat gcaagctata gattctgata
      121 cggtcaataa aagagaattg cttaacaatt ttgcaaaatg tattggcgag taagaaccgc
      181 atttggtact ttccgggcaa ccgccagacg attctttatt ggtaatgaga ataattaaca
      241 attaaagagc gtcgcgaaag aataatgtgt ctcgacaggg gagacacagt acgaatcgac
      301 ataaggtgat cgtctgaatc accagaataa ataaagtcgg tgatagtaat acgtaacgat
      361 aaagtaacct gacagcagaa agtctccgag cctgtgcagg gtcccaatcc gggattacac
      421 atgctggtta ataccagtaa ttataatgag ggagtccaaa aaacaatgac caacatcacc
      481 aagagaagtt tagtagcagc tggcgttctg gctgcgctaa tggcagggaa tgtcgcgctg
      541 gcagctgatg tacccgcagg cgtcacactg gcggaaaaac aaacactggt acgtaacaat
      601 ggttcagaag ttcagtcatt agatccgcac aaaattgaag gtgttccgga gtctaatatc
      661 agccgagacc tgtttgaagg cttactggtc agcgatcttg acggtcatcc agcacctggc
      721 gtcgctgaat cctgggataa taaagacgcg aaagtctgga ccttccattt gcgtaaagat
      781 gcgaaatggt ctgatagcac gccagtcaca gcacaagact ttgtgtatag ctggcaacgt
      841 tctgttgatc cgaacactgc ttctccgtat gccagttatc tgcaatatgg gcatatcgcc
      901 ggtattgatg aaattcttga agggaaaaaa ccgattaccg atctcggcgt gaaagctatt
      961 gatgatcaca cattagaagt caccttaagt gaacccgttc cgtacttcta taaattactt
     1021 gttcacccat caacttcacc ggtgccaaaa gccgctatcg agaaattcgg cgaaaaatgg
     1081 acccagcctg gtaatatcgt caccaacggt gcctatacct taaaagattg ggtcgtaaac
     1141 gaacgaatcg ttcttgaacg cagcccgacc tactggaaca acgcgaaaac cgttattaac
     1201 caggtaacct atttgcctat tgcttctgaa gttaccgatg tcaaccgcta ccgtagtggt
     1261 gaaatcgaca tgacgtataa caacatgccg atcgaattgt tccagaagct gaaaaaagag
     1321 atcccggacg aagttcacgt tgatccatac ctgtgcactt actattacga aattaacaac
     1381 cagaaaccgc cattcaacga tgtgcgtgtg cgtaccgcac tgaaactagg tatggaccgc
     1441 gatatcattg ttaataaagt gaaagcgcag ggcgacatgc ccgcctatgg ttacactcca
     1501 ccgtatactg atggcgcaaa attgactcag ccagaatggt ttggctggag ccaggaaaaa
     1561 cgtaacgaag aagcgaaaaa actgctggct gaagcgggtt ataccgcaga caaaccgttg
     1621 accatcaacc tgctgtataa cacctctgat ctgcataaaa agctggcgat tgctgcctct
     1681 tcattgtgga agaaaaacat tggtgtaaac gtcaaactgg ttaaccagga gtggaaaacg
     1741 ttcctcgaca cccgtcacca gggcactttt gatgtggccc gtgcaggctg gtgtgcagac
     1801 tacaacgaac caacttcctt cctgaacacc atgctgtcga acagctcgat gaataccgcg
     1861 cattataaga gcccggcctt tgacagcatt atggcggaaa cgctgaaagt gactgacgag
     1921 gcgcagcgca cagctctgta cactaaagca gaacaacagc tggataagga ttcggccatt
     1981 gttcctgttt attactacgt gaatgcgcgt ctggtgaaac cgtgggttgg tggctatacc
     2041 ggcaaagatc cgctggataa tacctacacc cggaatatgt acattgtgaa gcactaatgg
     2101 caatacgtgg ggcaggagtg tcctgctcca cggtgtctga tttttatcgc attacagaag
     2161 gcacaggcca gaaggtaggg caatgttaaa atttattcta cgtcgctgtc tggaagcgat
     2221 tccgacgcta tttattctta ttactatttc gttctttatg atgcgcctcg cgccgggaag
     2281 cccttttacc ggcgaacgta ctttaccgcc ggaagtgatg gccaatatcg aagcgaaata
     2341 tcatcttaat gatccgatca tgacacagta tttcagctac ctgaaacaac tggcgcacgg
     2401 tgatttcggt ccatcgttta aatataaaga ttattcggtc aatgacctgg ttgcatccag
     2461 ttttcccgtt tctgccaaac tgggagccgc cgcatttttc cttgcggtaa tactgggtgt
     2521 tagtgctggc gttattgtcg cattaaaaca aaacaccaaa tgggactata ccgtgatggg
     2581 gctggcaatg accggggttg ttatccccag ttttgtggtt gcgccattat tagtcatgat
     2641 atttgcgatc attttgcatt ggctgccggg cggtggctgg aatggtgggg cgcttaaatt
     2701 catgatattg ccgatggtgg cgttgtcact cgcttatatc gccagtattg cgcgtattac
     2761 ccgaggctct atgattgaag tattacactc caactttatt cgtactgccc gggcgaaagg
     2821 gttacctatg cggcggatca ttttacgcca cgcattaaaa cctgctctgt tacccgtgct
     2881 ctcttatatg ggccccgcat ttgtcggcat tattaccggt tctatggtta tcgaaaccat
     2941 ttatggtttg ccggggattg gacaactgtt cgttaatggc gcattgaacc gtgactattc
     3001 cttagtgtta agcctgacca tcctggttgg tgctttaacc attttgttta atgccattgt
     3061 cgatgtgcta tatgcggtta tcgacccgaa aatccgttac tgatactgga gctcgcgatg
     3121 atgttaagta agaaaaacag cgagacgctg gaaaatttca gtgaaaagct ggaggtcgaa
     3181 gggcgcagct tgtggcagga cgcacgtcga cgttttatgc ataaccgtgc ggcggttgcc
     3241 agtctgatag tgctggtgct gatcgcgtta tttgtaatcc tggcaccgat gctttcgcag
     3301 tttgcctatg acgatactga ctgggcgatg atgtccagcg ccccggatat ggagtccggt
     3361 cactactttg gtactgactc atccggtcgc gacctgcttg tgcgcgttgc gattggcggg
     3421 cgtatctcac tcatggtcgg tgttgctgcg gcactggtgg cagtggtcgt ggggacactt
     3481 tacggttcgc tttccggtta tctgggcggt aaagtggatt cggtaatgat gcgtctgctg
     3541 gaaatcctca actccttccc attcatgttc ttcgtcattt tgcttgtgac ctttttcggt
     3601 caaaacatcc tgctgatttt cgtggcgatt ggcatggttt cctggctgga tatggctcgt
     3661 attgtgcgtg gacaaaccct gagtctgaag cgcaaagagt ttattgaggc ggcacaagtt
     3721 ggcggtgtat cgacatcggg cattgttatt cgccacattg tgccgaatgt actcggtgtg
     3781 gtggtggtct acgcatcact tctggtgccc agcatgatcc tctttgaatc tttccttagc
     3841 ttcctggggt tgggtacgca agagccgtta agcagctggg gtgcattgct gagtgatggc
     3901 gcgaactcga tggaagtctc tccatggtta ttgttgttcc cagcgggatt cctcgtggtg
     3961 acgctgtttt gtttcaactt tatcggcgat ggcttgcgtg atgctctcga cccgaaagat
     4021 cgttaaggag tgcagccatg agcgtaattg aaactgcaac tgtgccgctc gcacaacaac
     4081 aggctgacgt actgctgaac gtgaaagatt tgcgtgtcac ctttagtacc ccggacggcg
     4141 atgtcacggc ggtcaatgat ttgaattttt ctctacgtgc cggagaaacg ctgggcattg
     4201 taggtgagtc tggttcgggt aaatcgcaaa ctgcatttgc gttgatgggc ctgttggctg
     4261 ccaacggacg tattggcgga tcggcaacct tcaatgggcg tgaaatcctc aatttgccag
     4321 agcgtgaact caataaactg cgcgctgaac aaatctcaat gatttttcag gacccaatga
     4381 cttcgttgaa tccctatatg cgcgtcggtg agcagttgat ggaagtgctg atgctgcata
     4441 agaacatgag caaagctgag gcgtttgaag agtcggtgcg gatgctcgat gcggtaaaaa
     4501 tgccggaagc acgtaaacgc atgaaaatgt acccgcacga attttctggt ggcatgcgtc
     4561 agcgagttat gattgcgatg gcattgctat gtcgacctaa gctgctgatt gcggatgaac
     4621 ccactactgc gctggacgtc accgtacagg cgcagatcat gacgctatta aatgaactga
     4681 agcgggaatt taataccgcc atcattatga ttacccacga tcttggcgtg gtggcgggga
     4741 tctgcgacaa agtgctggta atgtacgccg ggcgcacgat ggaatatggc aacgcgcgcg
     4801 atgtctttta tcaacccgtt catccttatt ctatcggttt gctcaacgcg gtgccgcgtc
     4861 tcgatgcgga aggtgaaaca atgttgacca tccctggtaa tccgccaaac ctgctgcgat
     4921 taccgaaagg ttgcccgttc cagccacgtt gtccgcatgc gatggaaatt tgtagtagcg
     4981 caccgccgct ggaagagttt acgcctggcc gtctgcgtgc ttgctttaaa ccggtggagg
     5041 aactgttatg aatgctgtaa ccgaaggaag aaaagtcctc ctcgaaatcg ccgatctgaa
     5101 agtgcacttt gaaatcaaag atggcaaaca gtggttctgg caaccgccga aaactctcaa
     5161 agccgtcgat ggtgtcacgc ttcgcctgta tgaaggggaa acattaggtg tggtagggga
     5221 atcgggatgc ggtaagtcca cctttgctcg cgccatcatt ggcctggtta aagcgaccgg
     5281 cggtcatgtt gcctggttag gtaaagagtt gctgggcatg aagcccgatg aatggcgtgc
     5341 cgttcgcagt gatattcaga tgattttcca ggatccgttg gcatcgctaa acccgcgtat
     5401 gaccatcggc gagatcatcg ctgaaccact gcgtacttat catccgaaaa tgtcacgcca
     5461 ggaagttcgc gagcgcgtga aggcgatgat gctgaaagtc gggttattgc ctaacctgat
     5521 taaccgctat ccgcatgagt tctccggtgg gcagtgccag cgtatcggga ttgctcgtgc
     5581 tcttattctt gaaccgaagc tgattatctg cgatgagccg atttcggcgc tggacgtgtc
     5641 aattcaggcg caggtggtca acctgctcca gcagctgcaa cgtgagatgg gattgtcatt
     5701 aatttttatc gctcatgacc tggccgtggt aaaacacatt tccgatcgtg tgttggtgat
     5761 gtatctcggc catgcggtag aactggggac ctatgatgag gtctaccaca atccactaca
     5821 tccttacacc agggcattga tgtcggcagt ccccatacct gatccggatc tggagaagaa
     5881 caaaaccatc cagttactgg aaggggaatt accgtcgccg atcaacccgc cttccggttg
     5941 tgttttccgt acctgttgcc cgattgccgg tccggagtgc gccaaaacac gtcctgtgct
     6001 ggaagggagt ttcagacacg ccgtttcttg cctgaaagtc gatccgcttt aaacacaata
     6061 agggctgaca gttgtcagcc ctttttcacg ccgaaagcga ttatttattc ccgccagatg
     6121 atatggcaaa gtttgtgatc tttttcgcgg cataacagaa tgcgggcgaa aatatcgttg
     6181 atttcaccat cctcactgtc cgccagacca atcaccactt cggcaaaaaa gtccgggttc
     6241 aaatcgaaat caacatgttc ctgccagtct tccgccggat cgaataactc cgcgccgccg
     6301 cgctcttcaa actgaagatt gaacagcaga acatcagctg ggtcgagatt gtcggcagcc
     6361 agttcgagaa aaatatcgta ggcctgctca agcgtttcat cttcagtcag gcgattgttt
     6421 agatccatat ccatagtcac tacctgttta acctctgttg gcgacgtttt acagcaacgg
     6481 gctgaagaag taaaacagtc gctcggcgac acgttgccat aatggacgtt ttagccataa
     6541 acgggcatcg agcagacgtg aacgcgaaat ataatcgtcc tgaacggcgg caaggtctgc
     6601 accaaaacct ttatcgtcga ttgccagggt aatctcgaaa ttcagccaca gactacgcat
     6661 atcaaggtta acggtgccaa ccagacttag ttcgccatcg accagcacgc tcttggtatg
     6721 cagtaacccg ccttcaaact gataaatttt aaccccagca gcaagcagtt ccgtaaagaa
     6781 tgcgcgactg gcccagccga ccagcatcga gtcatttttt cgcggaagga taatactgac
     6841 atccaccccg cgctgcgccg ccgtgcaaat cgcatgaagt aaatcatcgc ttggcacaaa
     6901 gtagggcgtt gtcatgatca aatattcacg cgccgaataa gccgcagtca ataatgcctg
     6961 gtgaatgaga tcttccggaa agccggggcc agaagcaatt gtgtgaatgg tgtgaccgct
     7021 ggcctgttca aacggcataa tattgacatc tggtggtggc ggcagaatac gttttccggt
     7081 ttcaatctcc cagtcgcagg aataaataat ccccatcgcg gtggcgatgg ggccttccat
     7141 acgcgccatc agatcaatcc attgccctac gcccgcatct tgtttgaagt agcgaggatc
     7201 gaccatattc atgctgccgg tgtacgcgat gtaattatcg atcatgatca tcttgcgatg
     7261 ttggcgcagg tccatacggc gtaaaaacac acgcatcaga ttgaccttta aggcttcgac
     7321 cacttcaata ccggcattac gcattagctc gggccacggg ctgcggaaaa aagccacact
     7381 cccggcggag tcgagcatca atcggcaatg aataccacgc cgtgcagccg ccattaatga
     7441 ttcagccacc tggtccgcca tgccgccggg ctgccagata taaaacacca tctcaatatt
     7501 atggcgcgcg agctggatgt cgcggattaa cgcctgcatc acatcatctg actcggtcat
     7561 cagttgtagc tgattccctt tgaccccagc gatcccctga cgacgctcgc aaagcttgaa
     7621 taatggcgca gcgacactgc tattttcttc ggcgaagata tgcttacagg ctttaaggtc
     7681 gttaagccat tttgcggtgg aaggccacat cgctctggcg cgctcagcgc ggcgtttgcc
     7741 taaatggagc tcgccaacgg caagataggc aataattccg actaacggca gaatgtaaat
     7801 aatcaacagc caggccatcg cggagggaac tgcgcgtcgt ttcattagaa tgcgtaaagt
     7861 tacgcctgca atgagcaacc agtatcccag aatggacaac caactcacca acgtataaac
     7921 ggttgtcata gattagaaat ccttttgaaa gcgcattgtt atgagtttac gcatatggat
     7981 tcatctggca aataaaaacg cgggaaaagc actggtcagc gtcgggggtg ggggtataat
     8041 gaccattctg ttattgcata gagtagttaa catgaagcgg agtagaacgg aagtggggcg
     8101 ctggcgcatg cagcgtcagg ctagccgacg taaatcgcgt tggcttgagg ggcaatcgcg
     8161 ccgaaatatg cgtatccaca gcatcaggaa gtgcattcta aacaaacagc gtaactcgtt
     8221 attgtttgcg atctacaata tctaaatata ctgggcacca ttgcggtgcc caataagttt
     8281 actctttcgt ctttaaactc tatttttgca ctgattcttt actgtcagct ttcgtttctt
     8341 gttcatcgtt atcgctgaaa atgccatgac cggagttcaa caacattgaa acgagcatat
     8401 cattattaat ctcttcacca tttaaaactc gcgccagaat ttcgctgcca aacagttgcg
     8461 gtgagagaat gatatccgga tgtaccatct taatcttatt caggtttttg ctatcactga
     8521 cggcgagaac tgttttgaca tcactgctca tatcttttgc cgagagtaca acaaacgcgt
     8581 tatctgcatc gttatcactc agcgccagaa tggcccggca acgatcgatt cccgctttct
     8641 ttaatactga actgtcatta ctgtcaccgg ggataacatc agcgttatcg cctaagcgtt
     8701 gctcaagttg cttgatatca tcttcaggca agttgctgat aaccgttacg ttttgtccgc
     8761 gttgattcag ttgcaggatc gtattgatgg cgagaatcga atgtccgcaa acaataaaat
     8821 gatctttacg atgcattgta tgattgtttc cttttacaag tttgttgaat cccccgcgga
     8881 taagcggacc aaaaattgag gtcatagatg tggcaaaaac ggtaatgccg gaaataatga
     8941 ccgaaatagt gaacaatcgt gctgattcag aaacagggac aatatcgcca tagccgacgg
     9001 ttgacatggt ttctatcgaa aaatagaacg cggtcatcaa actttctatt cgcggattaa
     9061 aaccttcgct taaataaagc gcaccgtagg ttgagtaaaa cagtaatgtc gtgaaactaa
     9121 taaatgcaaa aattgtcccg gctgcggcgc tactgtggga gaagtctttg cgcagtatca
     9181 gcaaaaacac cagcgtaaaa atgcaaaatc caatactaaa tttcagccag ggataaaaat
     9241 gcagggtgta aattagcgct atcaacaaca aaatgatact gattgcccat gcgagctttg
     9301 cccggaacag taagccaatg gagttcagga caagaaagac gccgagcata aatagtgggg
     9361 cgtttgccag caaggagaga tccaactctg aaaatgcttt gatatggaaa atatcaagca
     9421 aattgaccga cataccatag attgttttaa aaataagcaa tccatttaaa aagacggcca
     9481 gcgcgagaat gtcgtgccga agggtcaccc ataaatttgt tgctgtttgt ttgaatgtag
     9541 cccagtgact cactgatacc tctgtaacca caattttgtt taattatctt agcgtgcaca
     9601 ttttctgaac atacatgcag cgcgtgaatg tgttaaacaa tagtagatga ctttatccgc
     9661 agtaatcaaa ggatttgagc gagcaactgt acctcaggaa aggtcatttt tcctgaatat
     9721 gctcacatca tataaagaaa tacagataaa gttattatct gcttgtggta gtgaatgcac
     9781 tgaccggcta taag
//
LOCUS       DAZOZD010000152         9778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOZD010000152 DAZOZD010000000
VERSION     DAZOZD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.152"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUPB_003047"
     CDS             <1..347
                     /locus_tag="ACHUPB_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0759797.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUPB_003048"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUPB_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR0759798.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUPB_003049"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUPB_003049"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0759799.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHUPB_003050"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHUPB_003050"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0759800.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHUPB_003051"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHUPB_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0759801.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHUPB_003052"
     CDS             complement(4344..5309)
                     /locus_tag="ACHUPB_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0759802.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHUPB_003053"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHUPB_003053"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0759803.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHUPB_003054"
     CDS             6366..6746
                     /locus_tag="ACHUPB_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0759804.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHUPB_003055"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHUPB_003055"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0759805.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHUPB_003056"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHUPB_003056"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0759806.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2253 a         2478 c         2549 g         2498 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatctg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatc
//
LOCUS       DAZOZD010000153         9677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOZD010000153 DAZOZD010000000
VERSION     DAZOZD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.153"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUPB_003057"
     CDS             complement(<1..163)
                     /locus_tag="ACHUPB_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0759807.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUPB_003058"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUPB_003058"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0759808.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUPB_003059"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUPB_003059"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0759809.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUPB_003060"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUPB_003060"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0759810.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUPB_003061"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUPB_003061"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0759811.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUPB_003062"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUPB_003062"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0759812.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHUPB_003063"
     CDS             5058..6191
                     /locus_tag="ACHUPB_003063"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0759813.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHUPB_003064"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHUPB_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0759814.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUPB_003065"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUPB_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0759815.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUPB_003066"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUPB_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0759816.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUPB_003067"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUPB_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0759817.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUPB_003068"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUPB_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0759818.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUPB_003069"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUPB_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0759819.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2455 a         2475 c         2471 g         2276 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttatttt
//
LOCUS       DAZOZD010000154         9646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOZD010000154 DAZOZD010000000
VERSION     DAZOZD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.154"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..669)
                     /gene="ydcF"
                     /locus_tag="ACHUPB_003070"
     CDS             complement(<1..669)
                     /gene="ydcF"
                     /locus_tag="ACHUPB_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0759820.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(941..4843)
                     /gene="hrpA"
                     /locus_tag="ACHUPB_003071"
     CDS             complement(941..4843)
                     /gene="hrpA"
                     /locus_tag="ACHUPB_003071"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0759821.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5044..5649
                     /gene="azoR"
                     /locus_tag="ACHUPB_003072"
     CDS             5044..5649
                     /gene="azoR"
                     /locus_tag="ACHUPB_003072"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0759822.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5700..7016)
                     /locus_tag="ACHUPB_003073"
     CDS             complement(5700..7016)
                     /locus_tag="ACHUPB_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR0759823.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7006..8763)
                     /locus_tag="ACHUPB_003074"
     CDS             complement(7006..8763)
                     /locus_tag="ACHUPB_003074"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0759824.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8779..9646)
                     /locus_tag="ACHUPB_003075"
                     /pseudo
     CDS             complement(8779..9646)
                     /locus_tag="ACHUPB_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2628 c         2274 g         2296 t
ORIGIN      
        1 gctatcatcg cgtaaacgcg gcagctcgcc agtgagtagt gagagataac gctcaaccgg
       61 ccataatcct tgtaactgat taataaagat tacactgtct gcgttatttc ctaactgagg
      121 aacgaatccg ggataactta accagcgtgg tgcatcggga ttgtccccag tcatacggcg
      181 gaacgtcgcc atcgtgcgcc gctgcatggt ggggtcctga acaacgatag ccgtatgaac
      241 tcgttctacg gcctgattca atagcgcgat gctaaagcgt gcgttttcac cgcagtttgt
      301 tgactggtct tcaatccaga ttttttcatg cggaatgtgc cagaactgat gagcgatatc
      361 cgcctggatg gtcgcttctg ctctgccagt ggtgcggata gtgttgtagt gcggatgctg
      421 tgcgatggcg ctatacaaaa aagttgtcga gtgaccgata ccaccactaa tcagtaaagg
      481 aatttgctga tcgcgagcaa tcttacatgc cgcgtcgata gtcggcataa ccgcattgcc
      541 tgcaaggatc acgcaatcgg cctgatacgg cacctcaccg gagaaatcat cctgcgccag
      601 ccactgtccg ataacattta tggcatctat agttgccggc gaaagcgtcg gaaacggggt
      661 aatgttcatg gttcctcctt atcgttccct taagaatact gcgtgagata atagtgagaa
      721 gcggagcact ctgaaaggag gaagtcgtat ttccttaacc gccactccgc aagcctgaca
      781 tatcttttaa ggaaatgctg tagataaaca ttattcatct tgcagcagag aattgtattt
      841 ttctgaattc ctgaattttt acccttaccg ttttagaatt aataaaaaac ccggcgctaa
      901 atgcttaccg ggttttttct ttatcaggca aatagcaggg ttaaccgcta atctgctcca
      961 tcgcctgcaa aatacgctta tctgaaatcg gataaggcgt accaagttgt tgagcgaagt
     1021 aactaacacg caactcttct atcatccaac ggatctcttt cacgtcttca tcctcacgac
     1081 gtgcaggcgg cagtttgttg atccattgct gccacgcctg ctggacgttt tcgactttca
     1141 gcatctgagc acggtcgcga tgtggatcaa ccgccagttt ttccagccgt ttttcaatcg
     1201 cctgcaaata tcgcagcgtg tcgcccagcc gtttgaagcc gttaccagtg acaaaaccgc
     1261 gatataccag cccgcccatc tgcgctttaa tgtcagaaag ccccagcgcc atcgtcatat
     1321 ccacccgccc cttcagacgt ttgttgatat tgaacactgc cgtaaggatt tgctcgacct
     1381 gcttcgcaat atccaccacc gtgtcgttca gttcggcacg cactttttca tgcagcgcag
     1441 caaagccttc ttccgtccag accgggccac cattggcgtc gatcaattta tccacaccgc
     1501 aggagataca gtcgtcgatc agctccagca ctttgccata cgggttaaag tacagcccca
     1561 gcttggcttt gtttggtaac ttctcatgta aatacttgat cggcgaggga atattcagca
     1621 gcagtagacg gcgaagaccg ttccacatta cctgtttttg ctccagcgga ttatcaaaca
     1681 gtttgatcgc cacactgtcg cgttcatcca ctagcgctgg ccacgccttc actttgtagt
     1741 tgccacgctt ctgttcgtag ctttccggca gctgaccaaa actccagata tgcagtccgc
     1801 tctgctcgat accgtcatcc gccaccgcag acagcgtttc ctgcactttg cctttcagcg
     1861 catctttcag atcttgtagc gagcgccctt cttttagctt cttgtttttg tcatccacca
     1921 cgcggaaggt gattttcagg tgatcgggca cctgatccca gtgccagtct tcgcggtcaa
     1981 cggtaacgcc ggtcatccgc cgtaactcgc gctcaaggct gtcgagcaac ggtaactcca
     2041 gcggtgtgac gcggcctaaa aacgcttcgg cgtagtttgg tgcaggcaca aaattacggc
     2101 gtaccggttt cggcaacgat ttaatcagag caatcaccaa ttcgcggcgc agaccgggga
     2161 tctgccattc aaacccgctt tcctcaacct ggttaagtaa cggcagcgga atatgtacgg
     2221 tcacaccgtc cgcgtccgcg cctggctcaa actgatagct caaacgcagc ttgagattgc
     2281 cctgatgcca gaagttcggg taatccagct ttctgatttt ttccgcccct tctttgatca
     2341 acatgctctt ttcaaagttg agcaaatcag gcgtttcgcg gctgactttt ttccaccagc
     2401 tatcgaagtg acgagcggag attacatcgt ggctgatgcg ctggtcgtag aactcaaaca
     2461 gcgtttcgtc atcaaccaga atatcgcggc gacgtgattt gtgttccagc tcttccactt
     2521 cggcccgcag tttcaggttt tcacggaaga atgcgtgacg cgtctgccag tcaccttcca
     2581 ccagagcgtg gcgaataaag agttcacgac ataacgccga atcgatctgg ctgtagttga
     2641 ccttgcgcgc agcaacaatc ggcaaaccat aaacagtgac tttttccgtt gccatcaccg
     2701 cgccctgcgc ccgttcccag tgcggttcgc tgtaggtgcg tttaatcaaa tgctgagcaa
     2761 cgggttctac ccattccggg tcgatacgcg cagcaatgcg cccccacagg cggctggttt
     2821 ctaccagttc cgccaccatt acccatttcg gcggcttttt gaataaacca gaaccgggga
     2881 agatggagaa acgcgcgtta cgtgcaccgg tatattcctg tttatcggca tctttcatgc
     2941 cgatatggga aagcaaaccg gtcagtaagg cgatgtgaat ttcacgatac tccgccggtt
     3001 cgctgttgac cggaatgcca agttctttca ccacctggcg caactgggtg tagatatcct
     3061 gccattcgcg cacgcgcaga tagttgaggt aatcggtacg acacaggcga cggaaggcgt
     3121 tggaagaaag cgccttttgc tgctcgccaa gataattcca cagatttaca aacgcgagga
     3181 agtcggattc tttgtcgtgg aagcgacgat gtttttcgtc cgatgcctgc tgtttgtcca
     3241 tcggacgttc gcgcggatcc tgaatggaga gcgcggacgt gataatcatc gcctcacgca
     3301 cgcagccatg tttttgcgct tccagcacca tacgcgccag acgtgggtcg acaggcaact
     3361 gcgagagctg gcgaccgagc ggcgtcagtt tataggcgct ggcctgttca tccgtggtga
     3421 tcgcgcccag ttcttcgagc agacgcacgc catcctggat attgcgttta tccggtgctt
     3481 cgacaaacgg gaacgcagcg atatcgccca gccccagcgc ggtcatctgc aaaataaccg
     3541 aggccaggtt ggtacgcaga atctccggat cggtaaactc cgggcgcgag aggaaatcgt
     3601 cttcggaata gagacgaata cagatccctt cggacacacg accacagcgg cctttacgct
     3661 gattggcaga cgcctgcgaa atgggttcaa tcggcaaacg ctgcactttg gtgcgatagc
     3721 tgtagcggct gatacgcgct gtaccggggt cgatgacgta tttaatcccc ggtacggtca
     3781 gcgaggtttc cgcgacgttg gtcgccagca caatgcgccg tccgctgtgc gactggaaca
     3841 cgcggttctg ttcgctgttc gaaagccgcg cataaagcgg caagatttcg gtatggcgta
     3901 agttcagctt gttcagcgca tcggcggtat cgcgaatttc ccgctcacca ctcataaaga
     3961 tcagaatgtc gccagggctt tcctgactca gttcgtctac ggcgtcaaaa atcgcctgca
     4021 actgatcgcg ctcggtgtca tcggcttctt caacaatcgg tcgatagcgc acttccaccg
     4081 gataggtccg accggagact tcgataatcg gcgcattatt aaagtggcgc gaaaagcgtt
     4141 ccgggtcgat agtcgcggaa gtgataatga tttttaggtc aggacgccgc ggcagcaact
     4201 ctttcaaata gccgagcaga aaatcgatat tcaggctgcg ttcgtgcgct tcgtcaataa
     4261 tgatggtgtc gtactgcatc agcaggcggt cttgctggat ctccgccagc aggataccgt
     4321 cggtcatcag cttgaccatc gtgttatcac ttacgtgatc gctgaaacgc actttgtaac
     4381 cgatgcaacc gcccggctcc gttttcagct cttccgcaat acggttcgcc accgttcttg
     4441 ccgccaggcg acgcggctgg gtatggccga tcagcccttt aatcccgcgc cccagctcca
     4501 tacagatttt cggtaactga gtcgttttac cagaacccgt ttccccggcg acgatcacca
     4561 cctggtgatc acgaatcgct tcgagaatgt cctgtttttt ctgactaacc ggtaaattgt
     4621 caggataagt aatttccggt cgtgccgctt cacgcagcag gactttccct gccgcctggt
     4681 caatctcttt cgccatctcc tggaaaatgg cctgttgtgc atcaggattt ttaaccttct
     4741 tcacgccgtg cagacggcga gaaaaacgca gtctgtcacg cagcatcagc gaatccagcc
     4801 gctgctgcaa ggccgtaaag gtcaattttt gttgttctgt cataacgtta gtgggcagta
     4861 gtctgcctga ttaattttct ttgaaatgat agatatagag taccacatca cggctttcta
     4921 tgcgttgttc aataaattcg aacataggct tcgatttatt gcgctatctc tgtggcagga
     4981 ttgtgaataa agtgtcaaca agcaacgggg catcgcccat tcaaacatct ataaggaaac
     5041 accatgagca aggtattagt tcttaaatcc agcatcctgg cagggtactc tcagtctaat
     5101 cagttgtccg attattttgt tgaacaatgg cgcgaaaagc actccgctga tgaaatcacc
     5161 gttcgcgacc tggctgcaaa tccgattccg gtactggatg gcgaactggt tggtgcactg
     5221 cgtccgagcg atgcgccgct gactccgcgt cagcaggaag ctctggcgct ttccgatgag
     5281 ctgattgccg agctgaaagc ccacgacgtt atcgttattg cggcaccgat gtataacttc
     5341 aacatctcaa ctcagttgaa aaactatttt gacctggttg cacgcgcagg cgttactttc
     5401 cgctataccg agaacggtcc ggaaggtctg gtaacgggta aaaaagccat cgttattacc
     5461 agccgcggtg ggatccacaa agatggacca acggacctgg tgacgccgta tctgtccacg
     5521 ttcctcggct ttatcggcat tactgatgtg aaatttgtct tcgccgaagg gatcgcatac
     5581 ggtccggaaa tggcagcgaa agcgcagtct gacgcgaaag cagccatcga cagcattgtt
     5641 gctgcataac cggacacgat cttgatgtgg actgtagtcc agtccacatc ccaatccact
     5701 tacctgtttt cccataattt cagcatcgct ttgtgatctt ccttaagcac aatatgcgag
     5761 cgtctggctc gaataaagct aatcgcttca tcaacggttt tacagtatcc gtaacataac
     5821 agccatgccg ccactaccag cgcactgcgc gataatccca gtgcgcaatg gaccagaacg
     5881 ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg cctgtcggag ctccccctct
     5941 tccggaacca ccagatctag catcggtaca cagaaataga gtcgatcttt tgtcgctcgt
     6001 ccacgaggga attcaaaggt gacgtccaga accgcattct gtgccggaat atgtcgtgga
     6061 aacgccccta aataaacacc agcagtcatt ttgctcaccg gctccaggcg acgacaaaac
     6121 caacgcatag atagccacat cccgatgcgc cagggcaatg tcagccagta aacggcgggc
     6181 gggagtttcc cctgactatc tttccctgtt gttatcgcgc caagcccacc gtacccacgg
     6241 ccaataatga gtagcgataa tactggccaa cataaccaga ctgaccacca taactgaacc
     6301 atcatcatta gctccatcaa cacaatgcac gcgcacgcgc ctacgacata tggcagtgct
     6361 attttgattc gacgttgatc aggtttctga taattccaac gacggtcgac gggcaccatc
     6421 cagtcaatca acatacctac cgccagccct gtgatgacat caataaaatg atgctgccat
     6481 gtcgtcagcg tcgaaatggc gatgagtaaa aaccatccgc cgcagacttt acgccacctc
     6541 acagccagat gctgacgaaa gtgacgccaa agtagccagc agagaataat atgcagcgaa
     6601 ggagactggt tataaggcag atcaaacagt tcaagttgcg aaaatagcca tcccgtcacc
     6661 ccactcactt caggacggat aaaactaaac ttcagcggat agagcaaaaa accgcagcag
     6721 gccattaccg ttgccagaat aagccggtgg acaagtcggc gctgttcgaa tgtcgtgcta
     6781 caaacgaaca gcgaaaatcc atataaaaga tccagactcc agtaaggaac aatagtccag
     6841 ggaagaaaag ggatcgccgt ttcccaaccg aatacctgac tgggaatatc atggctatta
     6901 ctgtcctgaa ccgcggtgaa ctgattaaga gatccatagg tgaagaagaa aaacggggcc
     6961 agcaacaata accagccagc tccttgtagc aatacgttcc ggcgttcagt tatcacgacg
     7021 caccgccatc gaaaccgtaa aaatgcccca ctcatcaatc cgttgtgtgc atttatcaaa
     7081 tccagcatca tgcacgagca aatccatctc cccttgcgta cgcacgcgca ttacccacag
     7141 tttaccatct ttatgactgg ttaacacccc ggcaatcagc tccagttgtg ggtgccacgg
     7201 ctgcccggtg tagatgagaa tgccacccgg ttcgatggca ttggcaagac ctgcgagtga
     7261 gttttttacc tgctcatttt cgggaaacag ctcatacagt ccagagacaa tcgccagcgt
     7321 aggccgcgga gttaacgcgc tgagttcctc cgggttaaac gcatcgccct gttcaaaacg
     7381 cacccgccca gacattcccc gttgggcaat catctcttgc ccctgtgcaa catttaactc
     7441 actgtaatca cgtaataaaa tatcgcttac ggcaggctcg tttgccagtg catccagtac
     7501 atagcgccca tgccctgcgg caatgtcaac cacgcggacg gctaaacctt tgacgtggag
     7561 atcggcaacg gcttgtttaa tcagtatttg taaatgagtt ttgcgctggc gaataccgcg
     7621 ccagccaaca ctgttcaggt aaattttgtc gattaagcgc ccgaatgcgt tactgccttg
     7681 cggttgattt tgatagacat aatccagcga actgcctgaa tcaaagccag tgctcattcc
     7741 gagatgcagg cccgcagagt gcgtcccgaa caatttcatc gctttacgca taaagcgata
     7801 cgccaaatca accggcgata atggcacggg tccacctgaa aggaggcgcc agcgatccgc
     7861 tgatggtcca gtgcggtctt catgctgata atcaaatttt tgtgacttgt tagcatataa
     7921 acgacgaata aagctttgca ttttttcaaa tgcctgcgcc ctgttctctt cacccaacgt
     7981 gtcgtgataa aagcctggca gcagatgcag ctctttcaga gggctacgta atctctgata
     8041 aaaatcaatc tgcggttggc gatgcaccac atagtcatcg cctgatatca gaagttgcgt
     8101 ggggagcgta atcgccgccg catctctaat aatacgttca gacgttttat agagatcgag
     8161 caagatattg acggcaatcg cccgtgtgat cagcggatca ttattgaaac tcgccacccg
     8221 ttgccgatcg tgggtcaaat agcgtctttt cacataggaa ttaataaaaa acagaccacg
     8281 cagacgatgc catagcgtca gcgcaggacg tgccagcggc acatacaatt taaccttaaa
     8341 ggccggagaa gccagcacca gcccgcgaat tgcaggcgca taatcatgaa cccatgtggc
     8401 aaccagcact gcgccgacgc tttgcgcgat cacaaccacc tcttccagtc caacctggct
     8461 gtcgctggca gcaaaacgga caaattcatc gacatcctgc actgaacgcg caagagatgg
     8521 gctataacca cgcggccccg aagtttttcc atgcccccgg gcatcccatg cataaaaagc
     8581 agtatctggc atcgccagtt catcaacgat atgttgtaga cgaccagaat gttcatggcc
     8641 acgatgaaat aagacgatga cctttttcgc ccctggctgt aaagcgggcc agtgccgata
     8701 aaacaacgct gtattatcac tggtggtaaa aaaatgttcc ccagagatgc gtgaattttc
     8761 cattttctct attttccttc agtaacagca gtaacgaata aaataaaaaa ataccggagc
     8821 ggtgaaaatt aatgagtcaa tcctgtcaag gattccgcca tgtccaggta atagtttccc
     8881 gctatcttta acaccaacat ctcgtttgat ggctgacatc actacatcgc cgcaaaaacc
     8941 actaatacca attaacaaac ccgctaatat tgcctgtaat gtatttagcg gtgtcagtaa
     9001 cggtccaatg atcatcgacg ctatcatggt ggtgattacg ccgccgacca gaccttccag
     9061 cgtttttccc gggctgactt tggggaccac ttttcttctg ccgcaggatt tcccccataa
     9121 atactgtgca atatcgtttg acgcggttaa tgccaaaaga aataacacca gtaaggcccc
     9181 gccctggata ttcatggatg gcaacattaa taactaggcg gcatgactca aggcgaaaac
     9241 ggtcattatc cagccccaaa aaatggcgct cacggtatgc agaaaaccgg aggggtcacc
     9301 cacaaaaact cgccaggcgg ctaatatcag aaacccagcc aaaggtataa ataacagaaa
     9361 cagctcaaag caattaaaac cgataagcaa atagttaaga ggaataaccc aatataacca
     9421 ccgcggaaaa tgtacggata taagcataca atattctttc aatgccagaa aacttatcag
     9481 cgcaaaaaac gccaacgtca tccagcgtgg acctgaaatc accagtgaaa aacaaatgac
     9541 gatgccccac caggtacgaa tacgtaatgt aacctcaccc ccttttctct ccggtaaccg
     9601 ccatagcaga aaacggttta tcagcgttgc taaaattaac aacgcg
//
LOCUS       DAZOZD010000155         9621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOZD010000155 DAZOZD010000000
VERSION     DAZOZD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.155"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHUPB_003076"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHUPB_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHUPB_003077"
     CDS             2192..3328
                     /locus_tag="ACHUPB_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0759825.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHUPB_003078"
     CDS             3325..5325
                     /locus_tag="ACHUPB_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR0759826.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="ACHUPB_003079"
     CDS             5447..5908
                     /locus_tag="ACHUPB_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0759827.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="ACHUPB_003080"
     CDS             complement(5949..6419)
                     /locus_tag="ACHUPB_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0759828.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="btsR"
                     /locus_tag="ACHUPB_003081"
                     /gene_synonym="yehT"
     CDS             complement(6466..7185)
                     /gene="btsR"
                     /locus_tag="ACHUPB_003081"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0759829.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="ACHUPB_003082"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="ACHUPB_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0759830.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="ACHUPB_003083"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="ACHUPB_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9382..>9621
                     /locus_tag="ACHUPB_003084"
     CDS             9382..>9621
                     /locus_tag="ACHUPB_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0759831.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2354 a         2531 c         2468 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa acggtgctgt ggatgcgtta aaggaagaat taacccgacg catcagtaaa
     9481 cgttatgacg atgtagaggt gatcgtaaaa gccaccagca acgatggcct ttctgttaca
     9541 cgcaccgcag ataaggattc tgcaaaaaca tttgttcagg agactctgaa agatacctgg
     9601 gagtctgctg acgagtggtt t
//
LOCUS       DAZOZD010000156         9566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOZD010000156 DAZOZD010000000
VERSION     DAZOZD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.156"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            20..1345
                     /locus_tag="ACHUPB_003085"
                     /pseudo
     CDS             20..1345
                     /locus_tag="ACHUPB_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1348..2676
                     /gene="ssnA"
                     /locus_tag="ACHUPB_003086"
     CDS             1348..2676
                     /gene="ssnA"
                     /locus_tag="ACHUPB_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR0759832.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2727..3506
                     /gene="ygfM"
                     /locus_tag="ACHUPB_003087"
     CDS             2727..3506
                     /gene="ygfM"
                     /locus_tag="ACHUPB_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR0759833.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3503..6442
                     /locus_tag="ACHUPB_003088"
     CDS             3503..6442
                     /locus_tag="ACHUPB_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR0759834.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6375..6689
                     /locus_tag="ACHUPB_003089"
     CDS             6375..6689
                     /locus_tag="ACHUPB_003089"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0759835.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6725..7075
                     /gene="ghxQ"
                     /locus_tag="ACHUPB_003090"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6725..7075
                     /gene="ghxQ"
                     /locus_tag="ACHUPB_003090"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7144..7632)
                     /locus_tag="ACHUPB_003091"
     CDS             complement(7144..7632)
                     /locus_tag="ACHUPB_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0759836.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7632..9551)
                     /gene="ygfT"
                     /locus_tag="ACHUPB_003092"
     CDS             complement(7632..9551)
                     /gene="ygfT"
                     /locus_tag="ACHUPB_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR0759837.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2307 a         2564 c         2492 g         2203 t
ORIGIN      
        1 tgatccttaa ctgatcggca ttattcctca gttccgtatt cctgcagagt taattcagca
       61 cgatatcgat tttgttgccg ctcacggcgt gaaatttgag tatggctgct cacccgattt
      121 aaccgttgaa cagttaaaaa atcagggctt ccactatgtt ctgattgcca ccggcactga
      181 taaaaatagc ggtgtgaaac tggcgggcga caaccaaaat gtctggaaat cactcccctt
      241 cctgcgtgaa tacaacaagg gtacagcgct caagctgggc aaacatgtgg tcgttgtcgg
      301 ggcgggtaac accgcaatgg actgcgctcg tgcggcgtta cgcgttccag gcgtagaaaa
      361 agcaacgatc gtttaccgtc gttcactaca agagatgccc gcatggcgcg aagagtatga
      421 agaagcgttg cacgacggcg tagagttccg tttcctgaat aatccggaac gtttcgatgc
      481 tgatggcacc ttaaccttgc gcgttatgtc gcttggcgaa ccggatgaga aaggtcgtcg
      541 ccgtccggtt gaaaccaacg aaacagtaac actgcatgta gacagcctga tcaccgccat
      601 tggtgaacag caggatactg aagccctgaa tgcgatgggc gtgccgctgg acaaaaacgg
      661 ctggccagac gtcgaccata atggcgaaac tcgtctgact gacgtcttta tgatcggcga
      721 cgtacagcgc ggaccatcct ccattgtcgc tgctgtcgga accgcgcgtc gggcgaccga
      781 tgccatcctt agtcgggaaa atatccgttc ccaccagaac gataaatact ggaacaacgt
      841 caatccagcg gaaatctatc aacgtaaagg cgatatctct atcacgctgg tgaacagtga
      901 cgatcgtgac gcgtttgtcg cccaggaagc cgctcgctgc ctcgaatgta actacgtttg
      961 caacaagtgt gtggatgtct gcccgaaccg cgccaacgtc tccattgcag tccctggctt
     1021 ccagaataga ttccagacgc tgcacctcga cgcttactgt aacgaatgcg gcaactgcgc
     1081 tcagttctgt ccgtggaacg gtaaaccata cgaagacaaa atcaccgtct tcagcctggc
     1141 gcaagacttt gataacagca gcaccccagg cttccttgtg gaagattgcc gggtacgagt
     1201 acgtctgaat aaccaaagct gggtgttaaa catcgacagc gaaggtcagt ttaacaacgt
     1261 accaccggag ctgaacgata tgtgccgcat catcagccat gtccaccagc atcatcatta
     1321 tctgctgggc cgcgtggagg tgtaatcatg ttgattctga agaatgtcac cgcagtgcag
     1381 ttacacccgg cgaaagtgca ggaaggcgtt gatatcgcta tcgaaaatga cgtgattgtc
     1441 gctatcggcg atgccctgac gcaacgctac cccgatgcca gctacaaaga gatgcatggt
     1501 cggattgtga tgccgggaat tgtctgctcg cacaaccatt tttactcgga gctttcccgc
     1561 ggaattatgg caaacatcgc cccttgcccg gatttcatct caacgctgaa aaatctctgg
     1621 tggcggctcg atcgcgccct tgatgaagag tcgctctatt acagcggact gatttgttcc
     1681 ctggaagcga ttaagagcgg atgtacatcg gttatcgatc accatgcctc gccggcgtat
     1741 atcggcgggt cgctctccac attgcgcgac gcatttttaa aagttggcct gcgcgcgatg
     1801 acctgttttg aaactactga ccgtaacaac ggcatcaaag agttgcagga aggtgtagaa
     1861 gaaaacatcc gtttcgcccg tttgattgat gaggcgaaga aagcgacaag cgagccgtat
     1921 ctggtggaag cacatatcgg tgctcacgcg ccgtttaccg tgccggatgc cggtctggag
     1981 atgctgcgtg aagccgtgaa agccacaggc cgtggtttgc atattcacgc tgcggaagac
     2041 ctttacgacg tttcctacag tcaccactgg tacggcaaag acctgctggc acgactggcg
     2101 caattcgacc tcatcgacag caaaacgctg gtcgctcatg ggctgtactt gtcgaaagat
     2161 gacatcgccc tactcaatca gcgcgatgcg ttcctggtgc ataacgcccg ttcaaacatg
     2221 aacaaccatg tcggctacaa ccatcacctt agcgacatcc gcaacctggc attgggaacg
     2281 gacggcattg gttcggacat gtttgaagag atgaaatttg ccttctttaa acatcgcgat
     2341 gcgggtggtc cgctgtggcc tgacagtttt gccaaagccc tgactaacgg taacgaactg
     2401 atgagccgca actttggcgc gaaatttggg cttctggaag ccggttacaa agctgatttg
     2461 accatttgcg attacaactc gccgacgccg ctgctggcag acaatatcgc cgggcatatc
     2521 gctttcggta tgggctcagg cagcgttcac agcgtgatgg tcaatggtgt gatggtctat
     2581 gaagaccgtc agtttaactt cgattgcgat tccatttatg cacaagccag aaaagccgct
     2641 gccagtatgt ggcgtcggat ggatgcgctg gcataaatga cagatgccct cttcccgcag
     2701 ggaagagggt taaataagga aagaagatga ttgaacaatt tttcaggccc gactctgtcg
     2761 aacaggcgct ggaactgaag cgccgctacc aggatgaagc cgtctggttc gccgggggca
     2821 gcaaactcaa tgctacacca acccgtaccg ataaaaagat tgccatttcc ttacaggatc
     2881 tggaactgga ctgggttgac tgggataacg gtgcactgcg gattggtgca atgtctcgct
     2941 tgcagccact gcgtgatgcg cgctttattc ctgcagcact gcgtgaagcc ctcggttttg
     3001 tttactcacg ccatgttcgt aatcagtcga ccattggtgg tgaaatcgcc gcccggcagg
     3061 aagagtcagt gctgcttccc gtcctgctag cactggatgc tgaactggtt tttggcaacg
     3121 gcgaaacgct gtcaatcgag gactacctgg cctgcccatg cgatcgcctg ctaaccgaaa
     3181 ttatcattaa agatccgtat cgcacctgtg cgacccgcaa aattagccgt tctcaggcag
     3241 gtttaaccgt cgtgacggca gccgttgcaa tgacagacca cgacggtatg cgaattgcgc
     3301 tggatggcgt cgccagtaaa gcactgcgtc tgcacgatgt cgaaaaacaa aatctggaag
     3361 gcaatgcact tgaacaggct gtcgccaacg ccattttccc gcaggaagat ttgcggggca
     3421 gcgtggccta taaacgctat atcacgggag ttctggtagc cgacctgtat gccgactgcc
     3481 aacaggctgg ggaggaagcc gtatgatcat ccactttact ttaaatggcg cgcctcagga
     3541 gctaaccgtt aatccaggcg aaaacgtgca aaagctgttg tttaacatgg gaatgcactc
     3601 tgtacgcaac agtgatgatg gcttcgggtt tgccggttct gacgcaataa tctttaacgg
     3661 taatatcgtt aacgcgtcct tgcttattgc cgcacagtta gagaaggcag atattcgtac
     3721 cgcagaatct ctgggtaaat ggaacgagtt aagtctggtt caacaggcaa tggttgatgt
     3781 tggcgtggtg cagtctggtt ataacgatcc agctgcagct ctgattatca ccgatcttct
     3841 cgatcgcatc gccgcaccta cccgcgaaga gatcgacgac gcgctttctg gtttgttcag
     3901 ccgcgatgca ggctggcaac aatactatca ggtcattgaa ctggcggttg cacgtaaaaa
     3961 taatccgcag gccaccattg atatcgctcc gactttccgt gacgaccttg acgtcattgg
     4021 caagcattat cctaaaactg atgccgcgaa aatggtgcag gcgaaaccct gctatgttga
     4081 agactgcgta acggctgacg cctgcgtcat taaaatgtta cgtagcccac acgctcacgc
     4141 actgattact catctggatg tcagcaaagc tgaagcctta ccgggcgtcg ttcacgttat
     4201 tactcacctg aattgccctg atatctacta taccccgggg ggtcagagcg caccggaacc
     4261 gtcaccgctt gaccgccgta tgttcggcaa gaaaatgcgt cacgtcggcg atcgtgttgc
     4321 tgcggtcgtc gcagaaagtg aagaaattgc gctcgaagca ctgaagctca tcgacgttga
     4381 atatgaagtg cttaagccgg taatgtcgat cgacgaagca atggcggaag atgcgcctgt
     4441 cgtgcacgat gaaccggtgg tgtatgttgc tggtgcgcca gatactctgg aagacgataa
     4501 cagccatgca gcccagcgcg gcgagcatat gatcatcaac ttcccgatcg gttctcgccc
     4561 tcgcaaaaat atcgccgcca gtattcatgg tcatattggc gatatggaca aaggctttgc
     4621 cgatgccgat gtgatcattg agcgaaccta taactcaacg caggcacagc agtgcccgac
     4681 tgaaacacat atctgcttta cccggatgga cggcgatcgt ctggttatcc acgcctccac
     4741 ccaggtacca tggcacttac gccgccaggt cgcgcgcctc gtgggcatga aacagcataa
     4801 agttcatgtc attaaagagc gagttggcgg cggttttggt tccaaacagg acatcctgct
     4861 ggaagaagtg tgcgcctggg caacctgcgt gaccgggcgt ccggtactat tccgctacac
     4921 ccgtgaagaa gagtttattg ctaacacctc tcgtcacgtc gcgaaagtca ccgtcaaact
     4981 gggagcgaaa aaagatggtc gcctgacggc agtgaagatg gatttccgcg ccaacactgg
     5041 cccttacggc aaccactcac tcaccgtacc gtgtaacgga ccggcgctgt cgctgccgtt
     5101 atatccgtgc gataacgtcg atttccaggt caccacctac tacagcaaca tttgcccaaa
     5161 tggtgcttat cagggttatg gcgcaccgaa aggtaacttc gctatcacca tggcattagc
     5221 ggaactggct gaacagttac agatcgacca actggaaatt atcgaacgta accgggtaca
     5281 cgaagggcaa gagctgaaaa ttctcggtgc aatcggtgaa ggtaaagcgc cgacctccgt
     5341 tccttccgcc gccagctgtg cactggaaga gatcctgcgt cagggtcgcg agatgatcca
     5401 atggtcgtca ccgaaaccgc aaaatggtga ctggcacatc ggtcgcggcg tcgccattat
     5461 catgcagaaa tcgggtatcc cggatatcga tcaggctaac tgcatgatca aactggaatc
     5521 ggacggcacc tttatcgttc attctggcgg tgcggatatt ggtactggtc tggatactgt
     5581 agtgacgaaa ctggcagcag aagtgctgca ctgcccaccg caggacgtgc atgttatctc
     5641 cggtgatacc gatcatgcgt tgtttgataa aggcgcatat gcctcgtccg gtacttgctt
     5701 ctcgggtaac gcggcgcgtt tggcagcgga aaatctacgg gagaaaattc tgttccacgg
     5761 cgcgcaaatg ttgggtgagg cagtggcaga tgttcaactg gcaacgccgg gcgtcgtgcg
     5821 cggcaagaaa ggcgaagtta gtttcgggga tattgcccat aaaggcgaaa ccggcatcgg
     5881 ctttggttca ctggtgggaa ctggcagtta tatcacgcct gatttcgcct tcccgtatgg
     5941 cgcaaacttc gctgaagttg ccgtcaacac gcgtacgggt gaaatccgcc tggataaatt
     6001 ctacgccttg ctggactgcg gtacaccggt caacccggaa ttagcattgg gacaaatcta
     6061 cggtgctacc ctgcgagcta tcggccacag tatgagcgaa gagatcattt atgacgccga
     6121 aggtcacccg ttaacgcgtg atttacgcag ttacggcgca ccgaaaattg gtgacattcc
     6181 gcgtgatttc cgcgctgtgc tggtgccgag cgacgataaa gtcggcccgt tcggggcgaa
     6241 atcgatctcg gaaatcggtg taaatggcgc agctccggcg attgctaccg caattcacga
     6301 tacatgcggc atctgggcag aagaaagtca aagtgggcat gggaacggat atcggtgccg
     6361 gaacaacttt caacatgctg caaacgctga acgaagccta caaagtgttg caattacaag
     6421 gctatcgcct ctcggcatat gaagcgtttt acctggccac gctcggcgga gcgaaatctc
     6481 tgggccttga cgatttgatt ggcaactttt tacctggcaa agaggctgat ttcgtggtga
     6541 tggaacccac cgccactccg ctacagcagc tgcgctatga caactctgtt tctttagtcg
     6601 acaaattgtt cgtgatgatg acgttgggcg atgaccgttc gatctaccgc acctacgttg
     6661 atggtcgtct ggtgtacgaa cgcaactaat aataaaactt taacatcctc gtgaggacat
     6721 cattatgtct ggagacatcc tacaaacacc ggacgcacca aagccacagg gcgcgctgga
     6781 taattatttt aaaattaccg ctcgtggcag taccgttcgt caggaagtac tggctggctt
     6841 aacgaccttt ctggccatgg tttattccgt tatcgtcgtt ccgggaatgc tgggcaaagc
     6901 agtttttcct cccgcagctg tgtttgttgc cacctgtctg gtcgcgggct tcggctcgtt
     6961 gctgatgggg ttgtaggcca atttgccaat ggcgattggt tgtgcgattt ccttgacggc
     7021 atttaccgca ttcagtctgg tactcgggca acaaattagc gttcctgtcg cactggtcgc
     7081 attttacgcg ggtggttggg caatctaata gattctccgc gcctttcagc gcggagattt
     7141 ctttcacaga ggattctcac cgctggcaac aatacgctgc tggcggactc gcctgagttc
     7201 tctttcagta agcaactgca gcgcctgtgt cgggcaagat tcaacacagg ctggcccctc
     7261 ttcccgctgt tcacagagat cgcatttcac aatttgttgt cgggtatccc ccggcaacga
     7321 ctgaatggtg atcattccaa acgggcacgc actgacgcaa ctctgacagc caatacaacg
     7381 ggcagaattg gtctgcacca cctgctcgcc catcgtcagc gcccccacgg ggcaagcgcc
     7441 aacacaaggg gcgttttcac actgatggca catcaccggc gcactgatgc tgtccagtcg
     7501 ctgtaccttc agccggggta aaaagacatc ggcattcaac tcctgttctg aagggtgagc
     7561 gaccacacag gctacttcac aagtacggca gccaatacag tcagccggat taacgataat
     7621 taacgatttc atcacgatgc cttcgtatca aacagagtta acatatcgcg cgccgcctgc
     7681 cttcctgcgg ccattgcagt gacaaccaga tccgcgccat gaactgcatc accaccagca
     7741 aagacttttt tcagatgcgt ctgggtaggt aaatacccga cgtcaccggt ttgaatcagg
     7801 ccccatttat cgagtttaat tccgctgccc tgcaaccacg gcatggcatg cgcctggaaa
     7861 ccaaaggcca taatcagaac atcggcgggc aattcaaact ctgaacccgc taccggacga
     7921 ggacgacggc gaccatccgg ccccggctca cccatggcgg tacgaatcag gcccaccgca
     7981 gttaaacgcc catcttcgtc acaagcgata tattgcggtt gaacattgaa ctgaaactcg
     8041 acaccttcct cgcgcgcatt aaccacctct ttgcgcgagc ccggcatact gacttcatca
     8101 cgacgatacg cgcaggtcac gctggcagca ttgaggcgaa tggaagtccg caaacaatcc
     8161 attgtcgtat cgccaccgcc caataccacg actcgcttac cttccacgtc cgtcagcgga
     8221 tactcttcag actccggcaa tcccatgagc tggcgggtat gggcagtcag gaacggtaga
     8281 gcctgaatga caccgggcgc atcttcatgc ggcagatctg ctcgcatcat cccgtaagtc
     8341 cccacgccga tgaaaactgc atcatattca gaagttaaat cgctaaaggt aatatcgcgg
     8401 ccaatttcac agttaagatg aaagtcgatt cccattgcgg tgaatatctc tcgccgctgg
     8461 cttaataccg ttttatcgag tttgaaagga ggaatgccaa aagtcagcat accgccaatt
     8521 tctggatggc gatcaaagac atcaacctga actcccgcgc gcgccagaat atcagcacac
     8581 cctaaccctg ctggcccagc gccaatcacc gccacttttt cgatacgggg aacaactttg
     8641 ctgacatcgg gacgccagcc catcgccagc gcggtatcgg tgatgtagcg ttccagatta
     8701 ccgatagaga ctgcgccaga gtgatctttc aaagtacatg cgccttcaca aagacggtcc
     8761 tgtgggcata ccctgccgca gatttctggt aaggaactgg tctggtggca aagttctgcc
     8821 gcttcaataa tctttccttc ctgtaccaga cggatgtaat ccggaatagc gttatgcagc
     8881 gggcaatgcc agttgcagtt agctttttcg gcacaataaa cacagcggtc actctcataa
     8941 gtcgcttgca gtggatccag cccgcaatag atttcgccaa agtgggtttt ccgttcactc
     9001 gctgaaattt tatctgcgcc tttacgcgag ttaacgggga gcaacgctgc actgcgagat
     9061 ggctgagcgt ctgatgacgc ttttcctgct gccgttttgc gctggcgagc cacctttatc
     9121 cactgtaacc ctttatcgtc catcagtcgt aacgcctgtg ttgggcagac ttcaatacag
     9181 gcttgcgtgc cggaactgcg ctggttacaa aggtcgcatt tctgcgcaat cgtatcgacc
     9241 atctcaacga cgccaaaggg gcaagcgatt gcgcatcttt tacagccaat acatttttgc
     9301 tcgtccagtt gtacgctatc ggactggaaa gtcagagcat taaccggaca agccgtaaca
     9361 caaggggcat tgttgcagtg atggcaggcc accggattcg cagcctggcc tttccctaca
     9421 acgtggatac gcggtcgaaa gtcactgtga ctcagcggcc agttttcttg attgtgtgcc
     9481 accgcacagg caatttcaca agcatgacag cctatacatt ccgcagcttc agcagcgata
     9541 aacttattca tttgcatccc tttcat
//
LOCUS       DAZOZD010000157         9500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZOZD010000157 DAZOZD010000000
VERSION     DAZOZD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.157"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUPB_003093"
     CDS             <1..108
                     /locus_tag="ACHUPB_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0759838.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUPB_003094"
     CDS             complement(149..1231)
                     /locus_tag="ACHUPB_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR0759839.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUPB_003095"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUPB_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0759840.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUPB_003096"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUPB_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759841.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUPB_003097"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUPB_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0759842.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUPB_003098"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUPB_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR0759843.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUPB_003099"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUPB_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0759844.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUPB_003100"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUPB_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR0759845.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUPB_003101"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUPB_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0759846.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHUPB_003102"
     CDS             complement(7185..7448)
                     /locus_tag="ACHUPB_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0759847.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHUPB_003103"
     CDS             7560..8060
                     /locus_tag="ACHUPB_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0759848.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHUPB_003104"
     CDS             8080..8274
                     /locus_tag="ACHUPB_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR0759849.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHUPB_003105"
     CDS             8274..8564
                     /locus_tag="ACHUPB_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0759850.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHUPB_003106"
     CDS             8844..8996
                     /locus_tag="ACHUPB_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0759851.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHUPB_003107"
     CDS             9008..9373
                     /locus_tag="ACHUPB_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759852.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2493 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac
//
LOCUS       DAZOZD010000158         9432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOZD010000158 DAZOZD010000000
VERSION     DAZOZD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.158"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHUPB_003108"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHUPB_003108"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0759853.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUPB_003109"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUPB_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0759854.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHUPB_003110"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="ACHUPB_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUPB_003111"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUPB_003111"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0759855.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUPB_003112"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUPB_003112"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0759856.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUPB_003113"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUPB_003113"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0759857.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHUPB_003114"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHUPB_003114"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0759858.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHUPB_003115"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHUPB_003115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0759859.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHUPB_003116"
     CDS             complement(6556..7449)
                     /locus_tag="ACHUPB_003116"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0759860.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHUPB_003117"
     CDS             complement(7446..8099)
                     /locus_tag="ACHUPB_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0759861.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHUPB_003118"
     CDS             complement(8110..9369)
                     /locus_tag="ACHUPB_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0759862.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAZOZD010000159         9409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZOZD010000159 DAZOZD010000000
VERSION     DAZOZD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.159"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..980)
                     /locus_tag="ACHUPB_003119"
                     /pseudo
     CDS             complement(1..980)
                     /locus_tag="ACHUPB_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(1008..1628)
                     /locus_tag="ACHUPB_003120"
     CDS             complement(1008..1628)
                     /locus_tag="ACHUPB_003120"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0759863.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(1625..2506)
                     /gene="rnm"
                     /locus_tag="ACHUPB_003121"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(1625..2506)
                     /gene="rnm"
                     /locus_tag="ACHUPB_003121"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0759864.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            2644..2688
                     /gene="trpL"
                     /locus_tag="ACHUPB_003122"
     CDS             2644..2688
                     /gene="trpL"
                     /locus_tag="ACHUPB_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0759865.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    2665..2758
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            2780..4342
                     /gene="trpE"
                     /locus_tag="ACHUPB_003123"
     CDS             2780..4342
                     /gene="trpE"
                     /locus_tag="ACHUPB_003123"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0759866.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            4342..5937
                     /gene="trpD"
                     /locus_tag="ACHUPB_003124"
     CDS             4342..5937
                     /gene="trpD"
                     /locus_tag="ACHUPB_003124"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0759867.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            5938..7299
                     /gene="trpCF"
                     /locus_tag="ACHUPB_003125"
     CDS             5938..7299
                     /gene="trpCF"
                     /locus_tag="ACHUPB_003125"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0759868.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            7311..8504
                     /gene="trpB"
                     /locus_tag="ACHUPB_003126"
     CDS             7311..8504
                     /gene="trpB"
                     /locus_tag="ACHUPB_003126"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0759869.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            8504..9310
                     /gene="trpA"
                     /locus_tag="ACHUPB_003127"
     CDS             8504..9310
                     /gene="trpA"
                     /locus_tag="ACHUPB_003127"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0759870.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         2221 a         2494 c         2468 g         2226 t
ORIGIN      
        1 ttccggggcg ctaacagccg ctgtgagtga tggggtgata cgtgctaccc attctcttcc
       61 aaggtttgct gagactgccc aacctagcaa cgcgtaatcc agtttccgtg tttccccttt
      121 ccgccgacgc ccactgtgtg ttgctgcctc tttacgcgca agatagttaa cgatagcttc
      181 accgtgggca taacgttgct gcccagtttg tgtaaacttt ggcagaaaaa tataccccat
      241 gccagtgaga taaccggtca ggaaaacagc gctaaaataa cccgctggca tataccagta
      301 aaagaataca tctccgacat tgatgtagag agaatagaag gccacgcctg aacaaatcag
      361 tggtaaacaa ttcttccgtc ctcaaggatg cggccatttc gatatgtgtc gccctcttct
      421 ccagtaaaga gatcgatttc gctaaatgga ttacccgcga tatcagggaa cttgagtgaa
      481 aatcgggtct tatagatttc aaacggccag tggttaccag tcacgttcca gattaacaga
      541 tctgaatctc cctcacgcag gaaagcattt ttaacctgat aatgaatttg ataatgatag
      601 tcgccttgag gcaagaaacg ttgtttatcg cccacgacaa tactcattaa cccgatatgc
      661 cagtcatgat gccagggttc aggaatacca tcacgggtga cgccaacaat atgatagttc
      721 agcagtgcag cgtccccacc aggctgcatc cagagtcgtg gtaaatcacg ataaaatccg
      781 tgtttaatct gctgatttcg cgcctgaaaa tgaatatttt ctttgatgtc gagcacccca
      841 tccggttgca gagtggcttc aacatcaaag agcgaaatcc cttcttcata agaagggatc
      901 tccgtgtttt ctgcataact tagagatgat acaaatagcc ctgctatcaa cagcaaaatg
      961 cagcgaaact tccctgccat agtcttccat ccgtggaacg caatttgtta taagaaaggc
     1021 ttcacatcac ctacgccttc acgcaccacg accggcgtat catcggtaag atcaataacc
     1081 gttgtcggtt tctgcccgag atagccgccg tgaataatca aatctacctg tttttccaga
     1141 cggtctttaa tttcttcggg atcggattcg gtaaattcgc tgcctggcag cattagcgaa
     1201 gtggaaagca tcggttcgcc cagtgcttca agcaacgcct gcgcaatcgg gttagaaggc
     1261 acacgcatac cgatggtttt gcgtttttcc tgcaacagac ggcgtggcac ttccttcgtc
     1321 cccttcagga tgaaggtata gttgcccggc gtgttgtttt tcattaaacg aaacgcaacg
     1381 ttatcaacaa atgaataggt cgagagttca gaaagatcgc gacacatcag ggtaaagttg
     1441 tgaccgtccg gcagctggcg aatacgacaa atacgctcca tcgcgttttt atcttcaatt
     1501 ttacagccga gcgcatagcc ggaatcagtt ggataaacaa tcaccccgcc tttacgcacg
     1561 atctccaccg cctggttgat cagacgttgc tgtgggttat caggatgaat ataaaaaaac
     1621 tggctcataa ttccctctct gtggtgttct gcggctgttc ccatagctgc cagacgcctt
     1681 caacgcctgc gggcagccag agtttacgac ctagttcaat ccacgggcat ggctgatgaa
     1741 aatcagatcc ttgtgatgcc cataaatgat gctgacgcgc aagggccgcc agctgggtac
     1801 gttcattggg cgattgctgg cactgcgcga cttccatcgc gtcaccgtgg tgttcggcaa
     1861 aatgcgcaac caatcttttc agccatttag cactaagatt gtaccgccca ggatgagcta
     1921 gcaccgcctt accgccagaa tgatgaatga catcaatagc ttgttctatt gtacaccact
     1981 gtggcggaac gtatccggtt ttcccgcgcg ccagatattt tttaaagaca tccgccattg
     2041 aactggcttt gccgcacccc acaaggaaac gcgcaaaatg accgcgcgtc accgcaccgc
     2101 cctgcgccag tcgtcgcgcg ccttccagtg cccctggtat ttgcgctttt tcaagtcttt
     2161 cggcaatcag ttgcgcccgc tgattgcgcc gttctgtctg ctgcgcgagg aactcacaca
     2221 ttagcggatg agtaatatca atattcagcc cgacaatatg aatttcatga ttttcccaga
     2281 ccgtggaaat ttccacgccg ggaataagat tcagcgccag tcccgaacgt gaaatttcct
     2341 ctcttgctgg cacgattgca gctgtgttgt catggtcggt gatcgccagg gtaccgacgc
     2401 gcatctcgac tgcacggtgc accaatactt ctggcgtcag gcagccatcg gaagctgtgg
     2461 tatggctgtg caggtcgtaa atcactgcgt aattcgtgtc gctcaaggcg cactcccgtt
     2521 ctggataatg ttttttgcgc cgacatcata acagttctgg caaatattct gaaatcagct
     2581 gttgacaatt aatcatcgaa ctagttaact agtgcgcaag ttcacgtaaa aagggtatcg
     2641 acaatgaaag caattttcgt actgaaagct tggtggcgca cttcctgaaa cgggcagtgt
     2701 attcaccatg cgtaaagcaa tcagataccc agcccgccta atgagcgggc ttttttttga
     2761 acaaaattag agaataacaa tgcaaacaca aaaaccgact ctcgaactgc taacctgcga
     2821 aggcgcttat cgcgacaacc cgactgcgct ttttcaccag ttgtgtggga atcgtccggc
     2881 aacgctgctg ctggaatccg cagatatcga cagcaaagat gatttaaaaa gcctgctact
     2941 ggtagacagt gcgctgcgca ttacagcatt aggtgacact gtcacaattc aggcactttc
     3001 cggcaacggc gaagccctgc tggcactact ggataacgcc ctgcctgcgg gtgtggaaaa
     3061 tgaacaatca ccaaactgcc gtgtgctgcg cttcccccct gtcagtccac tgctggatga
     3121 agacgcccgc ttatgctccc tttcggtttt tgacgctttc cgtttattgc agaatctgtt
     3181 gaatgtaccg aaggaagaac gagaagcaat gttcttcggc ggcctgttct cttatgacct
     3241 tgtggcggga tttgaagatt taccgcaact gtcagcggaa aataactgcc ctgatttctg
     3301 tttttatctc gctgaaacgc tgatggtgat tgaccatcag aaaaaaaaca cccgcattca
     3361 ggccagcctg tttgctccga atgaagaaga aaaacaacgt ctcactgctc gcctgaacga
     3421 tcttcgccag cagctgaccg aagccgcgcc gccgctgccg gtggtttccg tgccgcatat
     3481 gcgttgtgaa tgtaatcaga gcgatgaaga gttcggtggc gtagtgcgtt tgttgcaaaa
     3541 agcgattcgc gctggagaaa ttttccaggt ggtgccatct cgccgtttct ctctgccctg
     3601 cccgtcaccg ctggcggcct attacgtgct gaaaaagagt aatcccagcc cgtacatgtt
     3661 ttttatgcag gataatgatt tcaccctgtt tggcgcgtcg ccggaaagtt cgctcaagta
     3721 tgacgccacc agccgccaga ttgagatcta cccgattgcc ggaacacgtc cacgcggtcg
     3781 tcgtgccgat ggctcgctgg acagagacct cgacagccgc atcgaactgg aaatgcgtac
     3841 cgatcataaa gagctttctg aacatctgat gctggtggat ctcgcccgta atgatctggc
     3901 acgcatttgc acccccggca gcagctacgt cgccgatctt accaaagttg accgttactc
     3961 ttacgtgatg cacctggtct cccgcgtggt cggtgagctg cgccacgatc tcgacgccct
     4021 gcacgcttac cgcgcctgta tgaatatggg gacgttaagc ggtgcgccga aagtacgcgc
     4081 catgcagtta attgccgagg cggaaggtcg tcgccgcggc agctacggcg gcgcggtagg
     4141 ttatttcacc gcgcatggcg atctcgacac ctgcattgtg atccgctcgg cgctggtgga
     4201 aaacggtatc gccaccgtgc aagccggtgc tggcgtagtc cttgattcta ttccgcagtc
     4261 ggaagccgac gaaacccgta ataaagcccg cgctgtactg cgcgctattg ccaccgcgca
     4321 tcatgcacag gagactttct gatggctgac attctgctgc tcgataatat cgactctttt
     4381 acgtacaacc tggcagatca gttgcgcagc aatgggcata atgtggtgat ttaccgcaac
     4441 catattccgg cgcagacctt aattgaacgc ctggcgacga tgagcaatcc gatactgatg
     4501 ctttctcctg gccccggtgt gccgagcgaa gccggttgta tgccggaact cctcacccgc
     4561 ttgcgtggca agctgcccat tattggcatt tgcctcggac atcaggcaat tgtcgaagct
     4621 tacgggggct atgtcggtca ggcgggcgaa attcttcacg gtaaagcgtc gagcattgaa
     4681 catgacggtc aggcgatgtt tgccggatta acaaacccgc tgccggtggc gcgttatcac
     4741 tcgctggttg gcagtaacat tccggccggt ttaaccatca acgcccattt taatggcatg
     4801 gtgatggcag tacgtcacga tgcggatcgc gtttgtggat tccagttcca tccggaatcc
     4861 attctcacca cccagggcgc tcgcctgctg gaacaaacgc tggcctgggc gcagcagaaa
     4921 ctagagccag ccaacacgct gcaaccgatt ctggaaaaac tgtatcaggc gcagacgctt
     4981 agccaacaag aaagccacca gctgttttca gcggtggtgc gtggcgagct gaagccggaa
     5041 caactggcgg cggcgctggt gagcatgaaa attcgcggtg agcacccgaa cgagatcgcc
     5101 ggggcagcaa ccgcgctact ggaaaacgca gcgccgttcc cgcgcccaga ttatctgttt
     5161 gctgatatcg tcggtactgg cggtgacggc agcaacagta tcaatatttc taccgccagt
     5221 gcgtttgtcg ccgctgcctg tgggctgaaa gtggcgaaac acggcaaccg tagcgtctcc
     5281 agtaaatctg gttcgtccga tctgctggcg gcgttcggta ttaatcttga tatgaacgcc
     5341 gataaatcgc gccaggcgct ggatgagtta ggtgtttgtt tcctctttgc gccgaagtat
     5401 cacaccggat tccgccacgc gatgccggtt cgccagcaac tgaaaacccg caccctgttc
     5461 aatgtgctgg ggccattgat taacccggcg catccgccgc tggcgttaat tggtgtttat
     5521 agtccggaac tggtgctgcc gattgccgaa accttgcgcg tgctggggta tcaacgcgcg
     5581 gcggtggtgc acagcggcgg gatggatgaa gtttcattac acgcgccgac aatcgttgcc
     5641 gagctgcatg acggcgaaat taagagctat caattgaccg ctgaagattt tggcctgact
     5701 ccctaccacc aggagcaact ggcaggcgga acaccggaag aaaaccgtga cattttaaca
     5761 cgcttgttac aaggtaaagg cgacgccgcc catgaagcag ccgtcgctgc gaacgtcgcc
     5821 atgttaatgc gcctgcatgg ccatgaagat ctgcaagcca atgcgcaaac cgttcttgag
     5881 gtactgcgca gtggttccgc ttacgacaga gttaccgcgc tggcggcacg agggtaaatg
     5941 atgcaaaccg ttttagcgaa aatcgtcgca gacaaggcga tttgggtaga aacccgcaaa
     6001 cagcagcaac cgctggccag ttttcagaat gaggttcagc cgagcacgcg acatttttat
     6061 gatgcgctac agggtgcacg cacggcgttt attctggagt gcaagaaagc gtcgccgtca
     6121 aaaggcgtga tccgtgatga tttcgatccg gcacgcattg cctccattta taaacattac
     6181 gcttcggcaa tttcggtgct gactgatgag aaatattttc aggggagctt tgatttcctc
     6241 cccatcgtca gccaaatcgc cccgcagccg attttatgta aagacttcat tatcgaccct
     6301 taccagatct atctggcgcg ctattaccag gccgatgcct gcttattaat gctttcagta
     6361 ctggatgacg accaatatcg ccagcttgca gccgtcgctc acagtctgga gatgggggtg
     6421 ctgaccgaag tcagtaatga agaggaactg gagcgcgcca ttgcattggg ggcaaaggtc
     6481 gttggcatca acaaccgcga tctgcgtgat ttgtcgattg atctcaaccg tacccgcgag
     6541 cttgcgccga aactggggca caacgtgacg gtaatcagcg aatccggcat caatacttac
     6601 gctcaggtgc gcgagttaag ccacttcgct aacggttttc tgattggttc ggcgttgatg
     6661 gcccatgacg atttgcacgc cgccgtgcgc cgggtgttgc tgggtgagaa taaagtatgt
     6721 ggtctgacgc gtgggcaaga tgctaaagca gcttatgacg cgggcgcgat ttacggtggg
     6781 ttgattttgg ttgcgacatc accgcgttgc gtcaacgttg aacaggcgca ggaagtgatg
     6841 gctgcggcac cgttgcagta tgttggcgtg ttccgcaatc acgatattgc cgatgtggtg
     6901 gacaaagcta aggtgttatc gctggcggca gtgcaactgc atggtaatga agatcagctg
     6961 tatatcgata cgctgcgtga agctctgcca gcacacgttg ccatctggaa agcattaagc
     7021 gtcggtgaaa ccctgcccgc ccgcgagctt cagcacgttg ataaatatgt tttagacaac
     7081 ggccagggtg gaagcgggca acgtttcgac tggtcactat taaatggtca atcgcttggc
     7141 aacgttctgc tggcgggggg cttaggcgca gataactgcg tggaagcggc acaaaccggc
     7201 tgcgccggac ttgattttaa ttctgctgta gagtcgcaac cgggcatcaa agacgcacgt
     7261 cttttggcct cggttttcca gacgctgcgc gcatattaag gaaaggaaca atgacaacat
     7321 tacttaaccc ctattttggt gagtttggcg gcatgtacgt gccacaaatc ctgatgcctg
     7381 ctctgcgcca gctggaagaa gcttttgtca gcgcgcaaaa agatcctgaa tttcaggccc
     7441 agttcaacga cctactgaaa aactatgccg ggcgtccaac cgcactgacc aaatgccaga
     7501 acattacggc tggcacgaac accacgctgt atctgaagcg cgaagatttg ctgcacggcg
     7561 gcgcgcataa aactaaccag gtgctcggtc aggctttact ggcgaagcgg atgggtaaaa
     7621 ccgaaatcat cgccgaaacc ggtgccggtc agcatggcgt ggcgtcggcc cttgccagcg
     7681 ccctgctcgg cctgaaatgc cgtatttata tgggtgccaa agacgttgaa cgccagtcgc
     7741 ctaacgtttt tcgtatgcgc ttaatgggtg cggaagtgat cccggtgcat agcggttccg
     7801 cgacgctgaa agatgcctgt aacgaggcgc tgcgcgactg gtccggtagt tacgaaaccg
     7861 cgcactatat gctgggcacc gcagctggcc cgcatcctta tccgaccatt gtgcgtgagt
     7921 ttcagcggat gattggcgaa gaaaccaaag cgcagattct ggaaagagaa ggtcgcctgc
     7981 cggatgccgt tatcgcctgt gttggcggcg gttcgaatgc catcggcatg tttgctgatt
     8041 tcatcaatga aaccaacgtc ggcctgattg gtgtggagcc aggtggtcac ggtatcgaaa
     8101 ctggcgagca cggcgcaccg ctaaaacatg gtcgcgtggg tatctatttc ggtatgaaag
     8161 cgccgatgat gcaaaccgaa gacgggcaga ttgaagaatc ttactccatc tccgccggat
     8221 tggatttccc gtctgtcggc ccacaacacg cgtatcttaa cagcactgga cgcgctgatt
     8281 acgtgtctat taccgatgat gaagcccttg aagccttcaa gacgctgtgc ctacacgaag
     8341 ggatcatccc ggcgctggaa tcctcccacg ccctggccca tgcgctgaaa atgatgcgcg
     8401 aaaacccgga aaaagagcag ctactggtgg ttaacctttc cggtcgcggc gataaagaca
     8461 tcttcaccgt tcacgatatt ttgaaagcac gaggggaaat ctgatggaac gctacgaatc
     8521 tctgtttgcc cagttgaagg agcgcaaaga aggcgcattc gttcctttcg tcacgctcgg
     8581 ggatccgggc attgagcagt cattgaaaat tatcgatacg ctaattgaag ccggtgctga
     8641 cgcgctggag ttaggcatcc ccttctccga cccactggca gatggcccga cgattcaaaa
     8701 cgccactctg cgcgcctttg cggcaggtgt gactccggca caatgttttg aaatgctggc
     8761 actgattcgc cagaaacacc cgaccattcc cattggcctg ttgatgtatg ccaatctggt
     8821 gtttaacaaa ggcattgatg agttttatgc ccagtgcgag aaagtcggcg tcgattcggt
     8881 gctggttgcc gatgtgccaa ttgaagagtc cgcgcccttc cgccaggccg cgttgcgcca
     8941 taatgtcgca cctatcttca tctgcccgcc aaatgccgat gacgacctgc tgcgccagat
     9001 agcctcttac ggtcgtggtt acacctattt gctgtcacga gcgggcgtga ccggcgcaga
     9061 aaaccgcgcc gcgttacccc tcaatcatct ggttgcgaag ctgaaagagt acaacgctgc
     9121 acctccattg caggggtttg gtatttccac cccggatcag gtaaaagcag cgattgatgc
     9181 aggagctgcg ggcgcgattt ctggttcagc cattgttaaa atcatcgagc aacatattaa
     9241 tgagccagag aaaatgctgg cggcactgaa agtttttgta caaccgatga aagtggcgac
     9301 gcgcagttaa tccccctgcc gccagttccg ctggcggcat tttaactttc tttaacgaag
     9361 ccggaaaaat cctaaattca tttaatattt atctttttac cgtttcgct
//
LOCUS       DAZOZD010000160         9397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOZD010000160 DAZOZD010000000
VERSION     DAZOZD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.160"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHUPB_003128"
     CDS             197..301
                     /locus_tag="ACHUPB_003128"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0759871.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHUPB_003129"
     CDS             complement(350..1603)
                     /locus_tag="ACHUPB_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0759872.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHUPB_003130"
     CDS             1710..2603
                     /locus_tag="ACHUPB_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0759873.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUPB_003131"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUPB_003131"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0759874.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUPB_003132"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUPB_003132"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0759875.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUPB_003133"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUPB_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR0759876.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUPB_003134"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUPB_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0759877.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUPB_003135"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUPB_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0759878.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUPB_003136"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUPB_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0759879.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="ACHUPB_003137"
     CDS             complement(8323..>9397)
                     /locus_tag="ACHUPB_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR0759880.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2438 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAZOZD010000161         9365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOZD010000161 DAZOZD010000000
VERSION     DAZOZD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.161"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            226..594
                     /gene="sufA"
                     /locus_tag="ACHUPB_003138"
     CDS             226..594
                     /gene="sufA"
                     /locus_tag="ACHUPB_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0759881.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            603..2090
                     /gene="sufB"
                     /locus_tag="ACHUPB_003139"
     CDS             603..2090
                     /gene="sufB"
                     /locus_tag="ACHUPB_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0759882.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASELGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            2100..2846
                     /gene="sufC"
                     /locus_tag="ACHUPB_003140"
     CDS             2100..2846
                     /gene="sufC"
                     /locus_tag="ACHUPB_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0759883.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            2821..4092
                     /gene="sufD"
                     /locus_tag="ACHUPB_003141"
     CDS             2821..4092
                     /gene="sufD"
                     /locus_tag="ACHUPB_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0759884.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            4089..5309
                     /gene="sufS"
                     /locus_tag="ACHUPB_003142"
     CDS             4089..5309
                     /gene="sufS"
                     /locus_tag="ACHUPB_003142"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0759885.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            5322..5738
                     /gene="sufE"
                     /locus_tag="ACHUPB_003143"
     CDS             5322..5738
                     /gene="sufE"
                     /locus_tag="ACHUPB_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0759886.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            5887..6891
                     /gene="ldtE"
                     /locus_tag="ACHUPB_003144"
     CDS             5887..6891
                     /gene="ldtE"
                     /locus_tag="ACHUPB_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0759887.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(6954..7190)
                     /gene="lpp"
                     /locus_tag="ACHUPB_003145"
     CDS             complement(6954..7190)
                     /gene="lpp"
                     /locus_tag="ACHUPB_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0759888.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(7500..8912)
                     /gene="pykF"
                     /locus_tag="ACHUPB_003146"
     CDS             complement(7500..8912)
                     /gene="pykF"
                     /locus_tag="ACHUPB_003146"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0759889.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(8920..9114)
                     /locus_tag="ACHUPB_003147"
     CDS             complement(8920..9114)
                     /locus_tag="ACHUPB_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759890.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         2375 a         2222 c         2490 g         2278 t
ORIGIN      
        1 tctttttcat cacagcctta atgctcctta gataataacc attatctaac aatgagatac
       61 ctaattctta gcgtgcctgt taacccacaa atcagggtct atgcttatta aaagcaggca
      121 aaaagcatgt tttacggtaa agcccctgcg tttgctgggt tgaactgata atcattatca
      181 ctagcatgct gttatacgct gaaagcgatg aagtgaggta aatcgatgga catgcattca
      241 ggaaccttta acccacaaga tttcgcctgg caaggcttaa cgctgacacc cgcagcggcg
      301 atacacatcc gtgagctggt ggcaaagcag ccgggtatgg tcggcgtgcg cttaggcgtg
      361 aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca gtgttagcga gccggacaaa
      421 gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg tcccgctgca agcgatgccg
      481 tttattgatg gcacggaagt cgatttcgtt cgtgaaggac ttaatcagat attcaaattt
      541 cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa gctttggggt ataggcggta
      601 ctatgtctcg taatactgaa gcaactgacg atgtcaaaac ctggaccggc ggcccgctga
      661 attataaaga aggattcttc acccagttag ccaccgatga gctggcaaag gggataaacg
      721 aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc ggagtggatg ctggagtttc
      781 gtctaaacgc ctatcgcgca tggctggaga tggaagaacc gcactggttg aaagcgcact
      841 acgacaagct gaattatcag gattacagtt actactcagc accatcgtgc ggtaattgtg
      901 acgacacttg cgcgtctgaa cttggcgcgg tgcagcaaac tggcgcgaac gcctttttaa
      961 gtaaagaggt ggaggcggcg tttgagcagt tgggcgttcc cgtgcgggaa ggcaaagagg
     1021 tggcggtgga tgccattttc gactctgttt cggttgccac tacttatcgc gaaaaactgg
     1081 cggagcaggg aattattttc tgttcctttg gtgaggcgat ccacgatcat ccggaactgg
     1141 tgcgtaaata tctcggcact gtggtgccgg gaaatgacaa cttctttgcc gcgcttaatg
     1201 cggcggtagc ctctgacggt acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg
     1261 aactttccac ctattttcgc attaacgcgg aaaaaaccgg gcagtttgag cgcaccattc
     1321 tggtggccga cgaagacagc tacgtcagct acattgaagg ctgttccgct ccggtgcgtg
     1381 acagctatca gttacacgcg gcggtggtgg aagtcatcat ccataaaaac gccgaggtga
     1441 aatattccac ggtacaaaac tggttccctg gcgataacaa caccggcggt attctcaatt
     1501 tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag
     1561 aaaccgggtc agcgattacg tggaaatatc ccagctgcat tttgcgcggc gataactcca
     1621 ttggtgagtt ttactcagtg gcgctgacca gcggtcatca gcaagcggat accggcacca
     1681 agatgatcca catcggtaaa aacaccaaat cgaccattat ctcgaaaggg atctctgccg
     1741 gacatagtca gaacagttat cgcggcttag tgaaaatcat gccgacggca accaatgcgc
     1801 gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa ttgtggggcg cataccttcc
     1861 cgtatgtcga atgtcgcaat aacagcgcac aactggagca cgaggcaacg acatcacgta
     1921 ttggtgaaga tcaactgttt tactgcctgc aacgcgggat tagcgaagaa gacgccatct
     1981 cgatgattgt taacggtttc tgcaaagacg tgttctcaga actgccgctg gaatttgccg
     2041 ttgaagcaca aaaactcctc gccatcagtc ttgaacacag cgtcggataa ggaataaaca
     2101 tgttaagtat taaagattta cacgtcagcg tggaagataa agctatcctg cgtggattaa
     2161 gcctcgacgt tcgtcccggc gaagttcacg ccattatggg gccaaacggt tcgggcaaaa
     2221 gtaccttatc ggcaacgctt gccgggcgag aagattatga agtgacgggc ggcacggttg
     2281 agttcaaagg caaagatttg cttgcgctgt cgccggaaga tcgtgcaggc gaaggcatct
     2341 ttatggcctt ccagtatccg gtggagattc ccggcgtcag taaccagttt ttcctgcaaa
     2401 cggcacttaa tgcggtgcgc agctatcgcg gccaggaaac gctcgaccgc tttgattttc
     2461 aggatttgat ggaagagaaa atcgctctcc tgaagatgcc ggaagattta ttaacccgtt
     2521 cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg
     2581 tgctggaacc ggagttatgc attcttgatg aatcggactc cgggctggat attgacgcat
     2641 taaaagtggt tgccgatggc gtgaactcgc tgcgtgacgg caagcgctca ttcatcattg
     2701 ttacgcacta ccaacgcatt ctcgactaca tcaagcctga ttacgtccat gtgctatatc
     2761 agggacgaat tgtgaaatcc ggcgatttca cgttggtcaa acaactggag gagcagggtt
     2821 atggctggct taccgaacag cagtaatgcg ctgcaacagt ggcatcactt gtttgaagct
     2881 gaagggacaa aacgctcacc gcaagcacag cagcatttac aacaattgct gcgtaccgga
     2941 ctgccgacac gtaaacatga aaactggaaa tatacgccgc tggaagggct gaccaatagt
     3001 cagtttgtca gcattgcggg agatatatcc ccacagcagc gtgatgcctt agcgttaacg
     3061 ttagacgccg tgcggctggt gtttgtcgat ggacgttacg tgcccgcgct gagcgatgca
     3121 attgaaggca gcggatatga agtgagcatt aacgacgacc gtcaggggtt acccgacgct
     3181 attcaggcgg aagtgtttct gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc
     3241 gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt taatgcatat cacccagggc
     3301 gtggcaggtg aagaggtgaa cactgcccat taccggcatc atctggatct ggcggaaggt
     3361 gccgaagcaa cggtgatcga acattttgtc agccttaatg atgctcgtca ttttaccggg
     3421 gcacggttca ctatcaatgt cgcagcgaac gcccacttgc agcatatcaa gctggcgttt
     3481 gaaaacccgc tcagtcacca ctttgctcat aacgatttgt tgctggctga ggatgccacc
     3541 gcatttagcc atagtttcct gctgggtggc gcagtgttac gacacaacac cagtacgcaa
     3601 ctcaatggcg aaaacagcac gctgcggatc aatagcctgg cgatgccggt gaaaaacgag
     3661 gtgtgtgata cccgtacctg gctggaacac aataaaggtt tttgtaacag ccgacagttg
     3721 cacaaaacta tcgtcagcga caaaggccgc gcggtattta acggtttgat caacgtcgcg
     3781 cagcacgcca tcaaaacgga tggtcagatg accaacaata atctgctgat gggcaaaatg
     3841 gcggaagtgg atacaaaacc gcagctggaa atctatgcag atgatgtgaa atgcagccac
     3901 ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct atctgcgctc gcgcgggatc
     3961 aatcagcagg atgcccagca gatgatcatt tacgcctttg ctgccgaact gacggaagca
     4021 ctgcgtgatg aggggcttaa acagcaggtg ctggcccgaa tcggtcaacg gctgccagga
     4081 ggtgcaagat gactttttcc gtcgacaaag tgcgggccga ctttccggtg ctttctcgtg
     4141 aggtgaacgg tttgccgctg gcttatctcg atagcgccgc cagtgcgcag aaaccaagcc
     4201 aggtgattga cgccgaggct gagttttatc gtcacggcta cgcggcggtg catcgcggta
     4261 ttcatacctt aagcgcccag gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc
     4321 tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt ccgcggcacg acggaaggga
     4381 tcaatctggt cgccaatagt tggggcaaca gcaacgtgcg ggcgggcgat aacatcatca
     4441 tcagtcagat ggagcaccac gctaacattg ttccctggca gatgctttgc gcacgcgttg
     4501 gcgcagagct gcgtgtgatc cctctcaacc ccgacggtac gctgcaactg gagacgctgc
     4561 ctacgctgtt tgatgagaaa actcgcctgc tggcaattac tcatgtctcc aacgtgctgg
     4621 gaacagaaaa tccgctggcg gaaataatca cgcttgcgca ccagcatggc gcaaaagtgc
     4681 tggtggatgg cgctcaggcg gtgatgcatc atccggtgga tgttcaggcg ctggattgcg
     4741 acttttacgt gttctccggg cataaactgt atggccccac cggaattggc attctttatg
     4801 tgaaagaagc cttgttgcag gagatgccgc cgtgggaagg gggcggttct atgatcgcca
     4861 ccgtcagcct gagtgaaggc actacctgga ccaaagcacc atggcggttt gaagccggta
     4921 cacccaatac cgggggcatt attggtcttg gcgcggcgct ggagtatgtt tcggcgctgg
     4981 ggcttaataa catagccgag tatgaacaga atctgatgca ttatgcgcta tcacagctgg
     5041 aatctgtacc ggatctcact ctctatggcc cacaaaacag gcttggcgtt attgctttta
     5101 atctcggtaa acaccacgcc tatgatgttg gcagttttct cgataattac ggcattgctg
     5161 tgcgtaccgg acatcactgc gctatgccat taatggccta ttacaacgtc cctgcgatgt
     5221 gtcgggcgtc gctggccatg tataacaccc atgaagaagt ggatcgtctg gtgaccggcc
     5281 tgcaacgtat tcaccgtttg ctgggataac agggaggcac tatggcttta ttgccggata
     5341 aagaaaaatt gctgcgtaat tttttacgct gcgccaactg ggaagagaaa tatctctaca
     5401 ttattgagct gggccagcgt ctgactgaat tacgcgacga agaccgaagc ccacaaaata
     5461 gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg ccagaatgcg cacggaatta
     5521 ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg gcttattgcg gtagtcttta
     5581 ttctctacga tcagatgacg ccgcaggata ttgtcagttt cgatgtgcgt ccgtggtttg
     5641 aaaaaatggc actcacccaa catctcaccc catctcgttc acaaggtctg gaagcgatga
     5701 ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac taaagggaca gctttcatcc
     5761 ggttggctca cattgccgga tggtcaatgt ttacactgac ctgcacagga gtgttttggt
     5821 ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag tctccggcaa atacaggagg
     5881 tttacaatga aacgcgcgtc tttgcttaca ctcacgctta tcggcgcttt tagcgccatc
     5941 caggctgcct gggcggttga ttatccgcta ccaccaaccg gaagccgact ggttgggcaa
     6001 aatcaaacgt atacggtgca agaaggggat aaaaaccttc aggctattgc ccgacgtttt
     6061 gatactgcgg caatgttgat ccttgaagcc aataacacta tcgtcccggt gccaaaacct
     6121 ggtacgacga taactattcc ttcacaactg ttattacctg atgcaccgcg tcaggggatt
     6181 atcgttaacc ttgcagagct gcgcctttat tattatccgc cgggagaaaa tattgtgcag
     6241 gtttatccaa taggtattgg attgcagggg ctggaaacgc cggtgatgga aacgcgtgtt
     6301 gggcagaaaa tccctaaccc aacctggacg cctacggcag gcattcgtca gcgttcgctg
     6361 gagcgtggca ttaaattacc gccagtcgtt cctgccggac caaataaccc gctaggacgt
     6421 tacgcactgc gcctcgcgca tggtaatggc gaatacctca ttcatggcac cagtgcgccg
     6481 gacagcgttg gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc ggatattaaa
     6541 gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga accggtgaaa
     6601 tattccgtgg aacctaacgg gatgcgttat gttgaagtac atcgaccact atcagcagaa
     6661 gaacagcaaa acgttcagac aatgccatac acactgccag caggctttac gcaatttaaa
     6721 gacaataagg ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat
     6781 ccggtttcgg tgagcagtgg agcaactccc gcagccagca atgcgccttc agtagagtca
     6841 gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg cgacgcagta gtggtaaata
     6901 gcagacaaaa aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg
     6961 cggtatttag tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca
     7021 gcctgaacgt cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc
     7081 agagtctgaa cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc
     7141 agagtagaac ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc
     7201 ctctagattg agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg
     7261 agaatatttt tttgatggga aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa
     7321 cgctgggttt tagataaaaa aactgagaga aaacaggatg cttccatcgg attcatctta
     7381 gataaagtga gattatatag tgaaatccga tttgattttt tattgcttct ggttatcgat
     7441 taaataaaaa agcgcccatc agggcgcttc gatatacaaa ttaattcaca aaagcaatat
     7501 tacaggacgt gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc accagaaacc
     7561 ataactacga cgtcaccttt gtgtgccaga ccgctctgca gagccagttc tttacccaga
     7621 cggtagaaat catcagtaga agtgatctct ttaaccagct gcggcacaac gcctttgctc
     7681 agtaccaact gatgagccgt tttttcgttg gtggtcagtg ccagtatggt ggcatccggg
     7741 aagtatttac gtactgcgcg agcagattta ccgccctggg tagcaaccac gatcagcgga
     7801 gcatccagtt tttcagcagt ttcaacggca ccacggcata ccgcttcggt aatgcgcagt
     7861 ttacggttgt cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt acgttcgcag
     7921 atggtcgcca tgatagaaac cgcttccagc gggtatttac cttttgcgga ttcaccagac
     7981 agcatcactg cgtcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc ttctgcgcga
     8041 gtcgggcgtg ggtttttgat catggaatcc agcatctggg tcgcagtgat aacgacttta
     8101 cgtgcacgga tacatttttc gatcatcatc ttctgggcga agataacttc ttctaccgga
     8161 atttctacac ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc gaggatttcg
     8221 tcgaagttgt tgaggccttc ctggttttcg attttggaga tgatgtggat gttttcgccg
     8281 ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag aacgcttacg aataaaggaa
     8341 gcagcaacaa agtctacgcc ttgttcgcaa ccaaagatca ggtcctgttt gtctttttca
     8401 gccagtgctg gcagggcaat ggaaacgcca ggcaggttca cacctttgtt ttcgcccagg
     8461 tcgccgttgt tcagcacttt acagataact ttgttacctt caatggcagt aacttccata
     8521 ccgatcagac catcgtcaac cagtacggtg ttgccaacag acaggtcagt agtgaaacct
     8581 tcatacgtta ccgcaaccat ttcgctgttg ccgataacag atttatcagt ggtgaaagta
     8641 aaggtctgac ccgctttcag agaaacgtcg ttaccgcctt ccagtttcat ggtgcggatt
     8701 tccggacctt tggtatcaag caggatagcg gcggttttac cagttttgct catcacgttg
     8761 cgcagattct gaatgcgctg accgtgttct gcatagtcac catgagagaa gttcagacgc
     8821 ataacgttca tgccagcgtc cagcatttta gctaacatct cttcagattc ggttttcggt
     8881 ccgatggtgc aaacaatttt ggtctttttc atgacagtct tagtctttaa gttgagaagg
     8941 atgggagaaa cttgctttct gggcgcactg ccggaaagcg aatccggtat tacgaaagtg
     9001 gtggtgcctt gctctaagga taggtgacat cgaaaaagcg tgcagaggaa tgtgtgcttg
     9061 tgtctcagcc caacgtgagt gcctgttttt gagtcgttga gttctacagt ccaaagtgct
     9121 gaaaccattc aagagtcaat tggcgcgcat tatacgctga aattaatcca aaaggaaaac
     9181 aaaaacagcg tttcaaaaaa tatacaactt gttagacaat aaattgttat caaagattta
     9241 acgttccagg gaaaaggtta cgcaaaaact tcgctgtttg cgcaacgtct ggtgccgaag
     9301 ttgtcagtgt gaggataagt gaaagttcat ccggtgtata aatatatcat cgctcaattg
     9361 ctaca
//
LOCUS       DAZOZD010000162         9283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOZD010000162 DAZOZD010000000
VERSION     DAZOZD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.162"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            69..1247
                     /gene="purT"
                     /locus_tag="ACHUPB_003148"
     CDS             69..1247
                     /gene="purT"
                     /locus_tag="ACHUPB_003148"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0759891.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(1303..1944)
                     /gene="kdgA"
                     /locus_tag="ACHUPB_003149"
                     /gene_synonym="eda"
     CDS             complement(1303..1944)
                     /gene="kdgA"
                     /locus_tag="ACHUPB_003149"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0759892.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(1981..3792)
                     /gene="edd"
                     /locus_tag="ACHUPB_003150"
     CDS             complement(1981..3792)
                     /gene="edd"
                     /locus_tag="ACHUPB_003150"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0759893.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(4027..5502)
                     /gene="zwf"
                     /locus_tag="ACHUPB_003151"
     CDS             complement(4027..5502)
                     /gene="zwf"
                     /locus_tag="ACHUPB_003151"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0759894.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            5840..6709
                     /gene="hexR"
                     /locus_tag="ACHUPB_003152"
                     /gene_synonym="yebK"
     CDS             5840..6709
                     /gene="hexR"
                     /locus_tag="ACHUPB_003152"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0759895.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            6837..8279
                     /gene="pyk"
                     /locus_tag="ACHUPB_003153"
     CDS             6837..8279
                     /gene="pyk"
                     /locus_tag="ACHUPB_003153"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0759896.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8339..9181)
                     /locus_tag="ACHUPB_003154"
     CDS             complement(8339..9181)
                     /locus_tag="ACHUPB_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR0759897.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         2320 a         2385 c         2266 g         2312 t
ORIGIN      
        1 cagcaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt aatcagggga
       61 tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat
      121 taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg
      181 tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat cgctcccatg
      241 tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa aaaccacatt
      301 atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt gaagaggaag
      361 gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc
      421 gtcgcctggc ggcagaagag ctgcagctgc ccacttccac ttatcgtttt gccgatagcg
      481 aaagcctttt ccgcgaggcg gttgctgaca ttggctatcc ctgcattgta aaaccggtga
      541 tgagctcttc cggcaagggg cagacgttta ttcgttctgc agagcaactt gctcaggcat
      601 ggaagtacgc tcagcaaggc ggtcgcgccg gagcaggccg cgtaattgtt gaaggcgtcg
      661 ttaagtttga cttcgaaatt accttgctaa ccgtcagcgc ggtggatggc gtccatttct
      721 gtgcaccggt aggtcatcgc caggaagatg gcgactaccg tgaatcctgg caaccacagc
      781 aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac
      841 tgggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca
      901 gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct caagatcttt
      961 cagagtttgc cctgcatgta cgtgccttcc tcggacttcc ggttggcggg atccgtcagt
     1021 atggtcctgc agcttctgcc gttattctgc cacaactgac cagtcagaat gtcacgtttg
     1081 ataatgtgca gaatgccgta ggcgcagatt tgcagattcg tttatttggt aagccggaaa
     1141 ttgatggcag ccgtcgtctg ggtgttgcgc tggcgaccgc tgagagtgtt gttgacgcca
     1201 ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc cggcaaaaaa
     1261 acgctacaaa aatgcccgat cgtagatcgg gcgttttgac ttttacagct tagcgccttc
     1321 tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc
     1381 cggaaccagc caggaaccac cgatgcacag cacgcttttc agcgccaggt agtcacggta
     1441 gttagccgga gaaataccac ccgtcgggca gaaacggacc tgggagaacg gacccgcgat
     1501 cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga actctttcaa
     1561 accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa ttagaggaat
     1621 agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg
     1681 tgcacccgct tcagtgactt ctgccagctg ctgtggattc agcaccgtac cggcacccac
     1741 aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac actcggtacg
     1801 cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca tcggcactgc
     1861 gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca ggattgattc
     1921 tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa atttgtcgtc
     1981 ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac gcaaggcgct
     2041 gaataattca cgtcctgtcc ccacgcgtga cgcgctcagg tcaggaatgt gcggttcgcg
     2101 agcagccagt tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc cattcacacg
     2161 aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt ctggtgttac
     2221 gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg taactaacgc
     2281 aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat gtaattctgg
     2341 cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc ggtccagcaa
     2401 acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg ctggcgcttc
     2461 aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc ccaggttacc
     2521 gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag cgatcacatt
     2581 gctgtcgagt gatttttccg ccccttcccg ccagtcctgt tcaccattat tcagccatgg
     2641 ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt catgcagcag
     2701 gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct ggaagtggtt
     2761 aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat cagaaaggtc
     2821 agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat
     2881 ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca ccaccacttt
     2941 ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca tgcgtgtaac
     3001 ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga
     3061 agagcctggc aactgcatcc ccataaattc caccaccatc tggttggtgt tggcagtacc
     3121 gtagaaagta catgttcccg gcgcatggta agacgcggct tctgactcca gtaaggccat
     3181 gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt tatttggcaa
     3241 accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa gcgacagggc
     3301 tgccatcgtc agacccggga caatcttgtc gcacacaccg aggaacagag caccatcaaa
     3361 catgttatgg gacagcccca ccgccgcaga catcgctatc acttcgcggc ttagcagcga
     3421 caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa caccgcccgc
     3481 aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg gatagtgttc
     3541 ataaggctgg tgcgcggaga gcatgtcgtt ataggaggtg atgatggcga tattgttacg
     3601 caacatgctt ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag
     3661 gttaccgcat gccaactgcg aacgatgaac ggtcgacgtt ttcgcttgtt ctatccgggc
     3721 gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta cgcataacaa
     3781 ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt cacaacgcgt
     3841 ttcattcaga ggatttatga gtgaaacgcc tgtaaccgga gctcataggg caaaaacgtt
     3901 tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa
     3961 aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa aggataagcg
     4021 cagatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg ccaccgaggc
     4081 aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca tcgcccacgc
     4141 ctcagtaatg gagtctaccc atttccaggc ttcttccact tcatcacgac gtacaaacag
     4201 tgcctgaata ccacgcatgg tttccagcag cagacgttca taggcatccg ccagatgcgt
     4261 ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt tatgtttgtg
     4321 gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag gttgcagacg
     4381 gatagtcagt ttattctgcg gcagatcctg ccacgactct ttaaacagat tcagttcagg
     4441 tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac cagtacgcag
     4501 gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg ccacgaaggt
     4561 ttctgtattg ctgctcttgt tcgcgccctc ttcttccaga tatcccggca cttttttgcc
     4621 ctgggcgaag cccgcagtat attgcccgcg tacggttttt tcgcgtacgt tggagcggtc
     4681 gatgcggcgc agggacttca gtactttcac tttttcatcg cggatgctgt ctgcgctcag
     4741 gtcagatggc ggagacatcg caatcatgca gagaatttgt agcaggtggt tctgaatcat
     4801 gtcacgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc ccacttcttc
     4861 tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca caaacaggga
     4921 gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat agtggtcgat
     4981 acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt cctgcgaggt
     5041 cgccagcgac gtccccagcg gtttctccat gactacgcgt gccggtttag cattcagttt
     5101 tgcctcgcca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt
     5161 aatggtgata cgattttttt gatccagcat cgcgccgaga cggttgaatg cagcagtgtc
     5221 attgacatcg agattacaaa aatccagacg tgcactcagg gtgtcccata aaccttcatc
     5281 aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca actttggtat atgccgcttt
     5341 atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct gaccggcttt
     5401 ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa
     5461 aatgaccagg tcacaggcct gggctgtttg cgttaccgcc atgtcattct ccttaagtta
     5521 actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca ctgtactgct
     5581 tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg gaaaagcgcg
     5641 caaagtgcgg caaaactgat aaaaaaatca tcgttttcca ttcggtaaaa atctgacact
     5701 gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta acacggcaca
     5761 caggcgtaat atctgacaaa actgcatttc gatttctttc agtgcggaaa tcgtcattac
     5821 ccgtgagtct ctttacatca tgaatatgct ggaaaaaatc cagtctcagc tggaacattt
     5881 gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata acgcgatcca
     5941 ttcgagtatt gctgctatgg cactggaagc caatgttagc gaaccgacgg tgaatcgttt
     6001 ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg cacagagtct
     6061 ggcgaatggc actccctatg ttaatcgcaa tgtcaatgaa gatgacagcg ttgaatcata
     6121 cacagggaaa atatttgagt ccgcaatggc aacgcttgat catgtccgtc attcactgga
     6181 taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt
     6241 cggattaggc tcttcagccg ccgttgccca cgatgcgatg aataagttct ttcgttttaa
     6301 tgttccggtg gtgtactccg atgatatcgt gctgcaacgc atgagttgta tgaattgtag
     6361 cgacggagac gtggtggtgc tgatttctca cactggaaga acaaaaaatc tggtcgagct
     6421 ggcgcagctg gctcgcgaaa acgacgcaat ggtgattgcc ctcacctctg cgggtacccc
     6481 gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata ctgacattta
     6541 tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg cgacaggatt
     6601 tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg aagcgctgaa
     6661 ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa aacgactgtc
     6721 actgtcctaa tcttatacga catccgaatg agattaattt atcgccatcg cggcgttatt
     6781 tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag tattacatgt
     6841 ccagaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca gatcgcgata
     6901 ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg
     6961 gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg
     7021 ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta tccaccttta
     7081 aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc aacctgggta
     7141 aaggtgaagg cgacaaagaa aaagtcggta tcgactacaa aggcctgcct gctgacgtcg
     7201 tgcctggtga catcctgctg ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc
     7261 agggcatgaa agtgttcacc gaagtcaccg tcggtggtcc cctctccaac aataaaggta
     7321 tcaacaaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca
     7381 ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca cgctgtggcg
     7441 aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg aaaattgttg
     7501 ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac atcatcctcg
     7561 cctctgacgt ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc gacccggaac
     7621 tggtcggcat tcagaaagcg ttgatccgtc gtgcgcgtca gctaaaccga gcggtaatca
     7681 cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt gcagaagtca
     7741 tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct gcagaaactg
     7801 ccgcagggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg
     7861 aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc gacaatgtgg
     7921 aagaagctat tgccatgtca gcaatgtacg cagctaacca cctgaaaggc gttacggcga
     7981 ttattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc
     8041 taccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct ctctatcgtg
     8101 gcgttacgcc ggtgcacttt gatagcgcta atgacggcgt agcagctgcc agcgaagcgg
     8161 ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt gtcacccagg
     8221 gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tattttaacg gtagagtaat
     8281 ctcacctgcc ccggccgctg atggccgggg tatttagcaa cacataaatt aggactactt
     8341 attcaatcca atcataatca cagccccctg taaaatgaat acagggatag cctgaatgga
     8401 gatctaaata gcttgcaaga cgtcgcatac ccgcttcctc tgcaacacta tgtccaataa
     8461 ctgccatcgg taagccacta tctttggagt attgtgtctg cacccatgtt ttaattgaat
     8521 cgttaatcgt aatataataa tcagcctgat attccatata ttgtattgga tcacaaaaac
     8581 agcctgcccc aatgccaaca gttctgacta ctctggcttt atctccataa aattgaatgc
     8641 caggaagatt atatattttc aaatttttgg caaaacgttt ggtaatgtta atggcactgt
     8701 cagggtctat agcatataca cagtacatct catcagaagc aacaatattt gtaacatcca
     8761 gttctaactg ctgagccaaa gccttagaca ttccgaacgt aggctctcga tccatgacat
     8821 catggcaacg aatgattacc aaattatttt tatgcaaaaa ctcctttttt tgctcaatta
     8881 tttttaaata actgagttca ccgcgagtat aatccattat ttttctggcc catgaaaact
     8941 caagatcgtc accctccaga tcagcataag aataaaatgt aggctcatgt gtaataatga
     9001 tattgacacc ttcaaaaaca gcctttctta aaacatccat tgaggcaatc cagcaggtcc
     9061 caattttatt aataagtgtc gcctcagcac caaaaataat tttatcaaca gttggttgcg
     9121 aaacagcaac cattgaattt aaaaaacaat taagatccgc ggctgtttta atatgatgca
     9181 ttcaacatcc ttcacaggtt tcatcagcaa taaaataatt tcaacatttc tgggaaacgt
     9241 tttgcccatg cagactcaga gtgggtatca cctccccata aat
//
LOCUS       DAZOZD010000163         9163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOZD010000163 DAZOZD010000000
VERSION     DAZOZD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.163"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(93..200)
                     /locus_tag="ACHUPB_003155"
     CDS             complement(93..200)
                     /locus_tag="ACHUPB_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0759898.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(603..1703)
                     /gene="chaA"
                     /locus_tag="ACHUPB_003156"
     CDS             complement(603..1703)
                     /gene="chaA"
                     /locus_tag="ACHUPB_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0759899.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1973..2203
                     /gene="chaB"
                     /locus_tag="ACHUPB_003157"
     CDS             1973..2203
                     /gene="chaB"
                     /locus_tag="ACHUPB_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0759900.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2361..3056
                     /gene="chaC"
                     /locus_tag="ACHUPB_003158"
     CDS             2361..3056
                     /gene="chaC"
                     /locus_tag="ACHUPB_003158"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0759901.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(3100..3453)
                     /gene="ychN"
                     /locus_tag="ACHUPB_003159"
     CDS             complement(3100..3453)
                     /gene="ychN"
                     /locus_tag="ACHUPB_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0759902.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3638..5032
                     /gene="ychO"
                     /locus_tag="ACHUPB_003160"
                     /gene_synonym="iatA"
     CDS             3638..5032
                     /gene="ychO"
                     /locus_tag="ACHUPB_003160"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0759903.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5033..5683)
                     /gene="narL"
                     /locus_tag="ACHUPB_003161"
     CDS             complement(5033..5683)
                     /gene="narL"
                     /locus_tag="ACHUPB_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0759904.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5676..7472)
                     /gene="narX"
                     /locus_tag="ACHUPB_003162"
     CDS             complement(5676..7472)
                     /gene="narX"
                     /locus_tag="ACHUPB_003162"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0759905.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7811..>9163
                     /gene="narK"
                     /locus_tag="ACHUPB_003163"
     CDS             7811..>9163
                     /gene="narK"
                     /locus_tag="ACHUPB_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0759906.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         2226 a         2250 c         2376 g         2311 t
ORIGIN      
        1 gagataaaga caaaaatggc cgcaggctgt tacccctgcg gccggtttcg ggcgcatatt
       61 gccatcacgg cagcctgacg cccgttttca ccttacttcc ggttacgcca ccagctgaca
      121 atcgctgcgg taataatccc cgccaggatc ggtgctgcca ggtcgtgcca gaaagtcatg
      181 gcaaactgcg cgagcgtcat atagccgcct tgttgtaatg acaacaaggc ggctattctt
      241 gaagtgtctg gtttcaagat tagcccccgt tttgttgtca ggttttacct ctcaacgtgc
      301 gggggttttc tctttccagc aaccaatgcc accagggata aagcccccgc aacattgcgc
      361 ctcaccggat aattccggct tggtgtggat actacgtctc aattcatctt cacttcatcc
      421 ctgaaatgtt tgcaataaag agtacattcc ggcttttcaa cagctgttgc agtggtttcg
      481 tgcaggttgt ggtacaggct cgcaattctg attaacgagc tgtccaggta gtatgaagaa
      541 atacaataag caggaattta tcttatctcc cccgatttcc gtcatcggag gagatataac
      601 tgtcaggcaa atatcgtcat caaataggcg gcaaacagtg ccagatgcgc tgcgccattg
      661 agcacgttag tacgtccggt ggagaaggag atatggcaca gcactaaaga ggccaccatc
      721 accaccattt ctggcgcacc aagtgcaaac tgtaattcgt tacccgtcat aaaggcaatt
      781 agcgtgacga caggtacggt aagcgaaatg gttgctaaca ctgaaccaaa gaacagattc
      841 attgcgcgct ggacctggtt gttcaacact gcttttaacg cacctaaacc ttccggcgac
      901 agaatcaaca gagccaccag gaagccagta aaggcgacag gggcattcat gctgtcgagc
      961 aatgtctcca gcgggctggc attcattttg gtgactgcaa taacggcaat cagatggata
     1021 atcaaccaga tagcatgcca cacgctgcta tgggcagacg gtttaccgtg gtgcgggtcg
     1081 tcgtcatcac tgtcatcttc gtgctcgtag acaaacaaac tttgatgcgt tttggtctgg
     1141 atcagcaaaa atacgccata cattgccgca gaaattaatg ctaccagtaa cgcctgaccg
     1201 gttgaaaaat tcgccgcagg cagagccatt ggaaatacca gtacgattat cgccaggggg
     1261 aacagcgcaa ttaaatactg cttgatacca aacagattca tatattgggt ggcaaactta
     1321 cgaccgccca acaataatga aaagccaacc agcccaccgg taacaatcat aatgattgaa
     1381 tagagcgtat cacgcattag cgttggcgcg gcgtcgccgg ttgccattaa agctgaaatc
     1441 aaactgactt caagaataac cactgaaagg ctaagaataa gcgaaccgta aggttctccc
     1501 aggcgatggg ctaatacgtc cgcatgacgg acaacactaa aggcgctact taaaatacca
     1561 ataagcgcaa gaagattgat ggcaatgacc actggtagtg tctggctgct tccccacagg
     1621 aacagcacta ccagcgccag aaccgggaaa ataagcgaag tctccttgtg gcgggttttt
     1681 accgcctctt gagcatttga cattatggtt atccctttgc agatgaattt atcgaaaatg
     1741 taaaaaatag gtaggaaaaa taacagaaag tgtctggata tcggtaacat tttacgaatt
     1801 tttaccccgc tgtcgatttt ttactcattg gggcataaaa ataagtatta cgtttagaca
     1861 atgtttgttt tagcgtttct taagaacagt ctgacatgaa aattcttatg ttttggcaag
     1921 agtagatatt gttgaccaca cttaatgttc aactttgtaa aaggagtcaa cgatgccgta
     1981 taaaacgaaa agcgatctgc cggaaagcgt aaagcacgtt ctaccgtctc atgcccagga
     2041 tatctataaa gaagcgttca acagcgcatg ggatcaatat aaagataaag aagatcggcg
     2101 tgatgacgcc agtcgcgaag aaaccgcgca taaagtggcc tgggctgctg tgaagcatga
     2161 atatgccaaa ggggatgatg ataaatggca taaaaaatcg taaaaccggt agcttagtta
     2221 aagctattcg tgcggtgttg ccttgcaagt ggtccgtgga ttgcatattg tcccgttagt
     2281 ggtttcaaaa tgagcggtaa aaatgtccgg aagacaccaa aaagttgtcg cagggaagta
     2341 tgcagtggcg gaggtgtaag gtgataacgc gtgatttctt gatgaatgcc gattgtaaaa
     2401 cggcatttgg tgccattgaa gaatcactct tatggtcagc agaacaacgg gcggcttcgc
     2461 tggcggcgac gctggcttgt cgacctaatg agggaccggt gtggatcttc ggttatggat
     2521 cgttgatgtg gaatccggca ctggagttta ccgaattatg caccggtaca ctggttggat
     2581 ggcatcgcgc gttttgcctg cgcctgaccg ccgggcgcgg gactgcgcac cagccgggac
     2641 ggatgcttgc actgaaagag ggcggacgca ccacaggcgt cgcctatcga ctaccagaag
     2701 agacgctgga gcaggaacta accctgttgt ggaagcgcga gatgattacc ggctgttatc
     2761 tgccaacctg gtgtcagctt gatcttgatg atggacgcac agtaaacgcc attgtgttta
     2821 ttatggaccc gcgacatcca gaatatgaat ctgacactcg cgctcaggtc atcgcgccgt
     2881 tgattgcggc ggcgagcggt ccgctgggaa ccaacgcaca atacctgttt tcactggaac
     2941 aggagcttat caaactggga atgcaggacg atgggctgaa tgatttgctg gtatcggtaa
     3001 aaaaactgct ggcggagaat tatccggatg gtgtgttacg gccgggattc gcctgagtaa
     3061 acttccctca tagtggggcg tcagacgccc cctcaaacat taaaatgtga gcactttatc
     3121 ggctgacagc gtccattgcg ccagttccac aagagtaccg atttccaccc catcaataag
     3181 aggaagtgtg ctaatcccgc gcccgtcggt acaggttttg cacaatttca ccggtacatt
     3241 ctgagcggta aggatctcca gcatttgctg aatgttgtag ccttcccctg gtttttgccc
     3301 gcgcaacccg gctgtgaccg catcagacat caggaacaga cgcagatcca gattgctctc
     3361 ctgctctcgt aacgcaatgg ccagccgcaa gctgttaaac aaggattcgc tcccgtaagg
     3421 tgcgccattg gcaacgatca cgattttttg cattatttac tcctgtattc agggaattag
     3481 acactcatct tctatcttac tgcttctgca gcgtctgacc aatcggtcac ctttttaagg
     3541 attttcctga aagcgcgaga aaatacgaca aaagttgcca gtaatcgtta ttctttaagg
     3601 ctatggtttt tcattattac cggaagttac cgacgttttg agccgtttcg ttcctcgcat
     3661 tatcccgttt tatttactct tgctggcggc aggcggtaca gctaacgcac aatctacctt
     3721 cgagcaaaaa gcggcaaatc cctttgataa taacaatgat ggtctgccgg atttaggcat
     3781 ggctcccgaa aaccatgatg gggaaaaaca ctttgctgaa attgtgaaag atttcggcga
     3841 aaccagtatg aatgataacg ggctggatac tggcgagcag gcaaaagctt tcgcattggg
     3901 aaaagtccgc gacgcgctta gtcaacaggt taatcagcac gtagagtcct ggctatcacc
     3961 gtggggaaat gccagtgttg acgtcaaagt ggataacgaa ggacatttca ccggcagtcg
     4021 tggaagctgg tttgtgccgt tacaagataa tgatcgttat ctcacctgga gccagcttgg
     4081 tcttactcag caggatgatg ggttggtgag caatgtgggc gttgggcaac gctgggcgcg
     4141 cggcaactgg ctggtgggtt ataacacttt ttatgacaac ttgctggacg aaaatcttca
     4201 gcgagcgggc tttggtgccg aagcgtgggg cgaatatttg cgactatcgg caaactttta
     4261 tcagccgttt gctgcatggc atgaacagac agccacgcag gaacaacgga tggcgcgcgg
     4321 gtatgacctg acagcccgga tgcgcatgcc gttctatcaa cacctcaata ccagtgtcag
     4381 ggtagaacag tattttggtg agcgtgttga tttgtttaac tctggtacgg gttatcacaa
     4441 tcccgtcgcg ttgagtctgg gattaaatta cacccctgtg ccattagtca ctgtgacggc
     4501 ccagcataaa cagggtgaaa gtggcgaaaa tcaaaataac ctcggactga atcttaatta
     4561 ccgctttggt gtgccgctca aaaaacaact ttctgcgggc gaggttgccg aaagtcagtc
     4621 gttacgtggt agtcgctatg ataatccgca gcgaaataat ctaccgactc ttgagtaccg
     4681 acagcgaaaa acgttaacgg tgtttctggc gacaccgccg tgggatctaa aacctggcga
     4741 aacagtgccg ctgaaattac aaatccgcag tcgttacggt attcggcaac tgatttggca
     4801 gggcgatacg cagatattaa gtttgacgcc gggcgcacaa gccaacagcg cggagggctg
     4861 gacgctgatc atgcctgact ggcagaacgg ggaaggggcg agcaatcact ggcgattgtc
     4921 ggtggtggtg gaagataacc aggggcagcg tgtctcctcc aatgagatca cgctaacgct
     4981 tgtcgaaccg ttcgatgcat tgtcaaacga cgaactgcgc tgggaaccgt aatcagaaaa
     5041 tgcgctcctg atgcacccat accgctgctt ccacgcgaga cttgagcttc attttcttca
     5101 gcatgtgctt gacgtgcact tttactgtgc tttcggtgat atccaggcgg cgggcaatca
     5161 tcttgttcgg caaaccctgg gcaatcagct tgagaatatc gcgctcgcgt ggggttaact
     5221 ggttaacatc gcgctcagta gtggcacggt tagcgcgcaa gctggcggcc agaacaggcg
     5281 ttaatgcttc gcttaatacc atttcgccag cagctgcctg atgcaatgct ttcagcagat
     5341 cttccggttc catatctttt aacagataac catccgcgcc gcgtttcagt gcggtgacca
     5401 catcttcttc atggttagag acgctgaata ccacaatgcg ccctgagagg gacttttcgc
     5461 gcagtttatc cagcgtttcc agaccgttca tgccgggcat attgagatct aacaggatca
     5521 gatcgggatc aagagattcc gccagttcaa taccctgttc gccattactc gcttcgccaa
     5581 ccacggtgat atctggtgcc atactgataa gctgttttac gccagttcgc agcatcgggt
     5641 gatcgtcaat cagcaggata gtagccggtt cctgattact catgggtatc tccttggacg
     5701 tctgtgaaag ttttttcggg aataaaggtg accaccactt cggtgccacc tgattcacga
     5761 cggcggacgc ggcaatcgcc tcgtaaactt tgcgcacggt cgcgcattat tatcatgccg
     5821 tagtgattgc tgcggatggc attttcaggc acgccgcagc cgttatcctg gacggtcagt
     5881 ttgacctgat tatcgttttg cgccaccgtc accacgactt cactcgcttg cgaatgtttg
     5941 agggcgttac ttaatgcctc acgggcaatt tgcaacaagt ggattgcctg atgcgaaggc
     6001 accaggcgag gcggcaattg ataatccagc ttcaccggga agccaaattt ggcgctgtac
     6061 tcttcgcaac tcgcctccag cgccggacgt aatccaggct cggtgagctg caagcggaat
     6121 gtggtgagca attcacgcaa ctgcgcccag gatgcattca gttcgttacg gatctgactt
     6181 aacagttcgc ggctgctttc tggcagcgca tcgccctgca tctgtaaaca actcacctgc
     6241 atcttcatgc aagagagaga ttgggcaata gaatcatgca gttcgcgcgc aatggtggca
     6301 cgctcttcca tcacgatcaa ctgttgctga cgttcctgat ggcgatccag cgccagcgtg
     6361 gcggtgagtt gttcaaccag ggtatccagc agttgttgtt gatcatggct aagatgacgc
     6421 ccctgcggca gggtcgccag caaaataccg tactgcgtat gagagtcagc cagccgccac
     6481 ttcagggtcg tgccgcgatc accaacgggt aatacgccgc gcgggcagag ctggcagcct
     6541 ttatcatcac aagtcatatc tggctggcag gtaaactcct gatgattctc ttcatcatca
     6601 gtgtcataca cccgcaattc gatatcacgt agcagggtta aattctgtaa gccgttgagt
     6661 acaggtgaca ggcgttcaca cagcggggcg cgggaatgca aacggcggtt agcctgccat
     6721 aaaaaagaga ggatctgatt tttatgctcc agcccggcgg ttttctcctg aacccgctgc
     6781 tcaagtacgg cataactttc ggccagttct gcagacatat tgttcaatgc agttccaagc
     6841 atcgccattt cgttgcgccc gctgatgttc gcgcgttggg taaaatcgcg atgactgacg
     6901 gcactcgcca ttgccagcag ttgccgccac ggttgtagca gtcgcgcccg caaccagata
     6961 atagtgaaca ccagtaaaag tgccataaat accgccatta cccgatggac cagtaccact
     7021 gtctcaatgc gcatttccgt ggtgcggtca aaaccagata ccagctgatc aagcccggca
     7081 acaaactggc tgacatccgc tgacaccgtt tcgcggtttt gtgcacgcat cagtgcaggg
     7141 atcagttcat tacgccagta atcttgtaaa ccctgtaatt gcgccagttg ttcgtctcgt
     7201 tctgctgctc gagttaactc ggcgctaaat gccgtttgtt ccatctcttt aattaagggc
     7261 ttgtctttct cacttaatgg cactgccgcc aacagacggt aactttgcat gcgcagcgat
     7321 cccgctttgt tgatcgcatg ggcgctgccc tgaacgcctt gcaccagcca gccagaaacc
     7381 gccatccctg ccagtccaat agcagtagaa agcaacacaa taagcgcaac ctgattaacc
     7441 agggtgagcg gagagagaca acgtttaagc atgcaaacct cttccttcag gctttaaatg
     7501 agcaataacc ttaatgaatg tgacgataca ttctggaatg gcagtattct cggctattgg
     7561 ctgaagtata cccatacccg gaaagagtta ctccttattt gccgtgtggt tagtcgcttt
     7621 acatcggtaa gggtagggat tttacagcac cgtgaaaaat ctcataattt ttatgaaatc
     7681 actgtactca ctatgggtaa tgataaatat caatgataga taaagttatc ttatcgtttg
     7741 atttacatca aattgccttt agctacagac actaaggtgg cagacatcga aacgagtatc
     7801 agaggtgtct atgagtcact catccgcccc cgaaagggct actggagctg tcattacaga
     7861 ttggcgaccg gaagatcctg cgttctggca acaacgcggt caacgtattg ccagccgtaa
     7921 cctgtggatt tccgttccct gtctgctgct ggcgttttgc gtatggatgt tgttcagcgc
     7981 tgttgcggtg aacttaccga aagtcggttt taattttacg accgatcagc tatttatgtt
     8041 gactgcgctg ccttcggttt ctggcgcgtt attacgtgtt ccatactcct ttatggttcc
     8101 tatcttcggt ggtcgtcgct ggacggcgtt cagcaccggt attctgatta ttccttgcgt
     8161 ctggctgggt tttgccgtgc aggatacctc cacgccttat agcgtcttca tcatcatctc
     8221 tctgctatgc ggctttgctg gcgcgaactt cgcatccagt atggcaaaca tcagcttctt
     8281 ctttccgaaa cagaagcagg gtggcgcgct gggtctgaat ggtggtctgg gaaacatggg
     8341 cgtcagcgtc atgcagttgg ttgctccgct ggtggtatca ctgtcgattt tcgcagtatt
     8401 tggtagccag ggcgtcaaac agccggatgg gactgagctg tatctggcga atgcgtcctg
     8461 gatatgggtg ccgttccttg ccatcttcac cattgcggcg tggtttggca tgaacgatct
     8521 tgctacctcg aaagcctcca tcaaggagca gttgccggta ctcaaacggg gtcatctgtg
     8581 gattatgagc ctgctgtatc tggcaacctt cggctccttt atcggcttct ccgcgggctt
     8641 tgcgatgctg tcaaaaacgc agttcccgga tgttcagatt ctgcaatacg ctttcttcgg
     8701 gccgtttatt ggtgcgctgg cgcgttctgc aggtggtgca ttatctgacc gtctgggcgg
     8761 aactcgtgtc acgctggtga actttattct gatggcgatt ttcagcggcc tgctgttcct
     8821 gaccttaccg actgacgggc agggcggaag cttcatggcg ttcttcgcgg tcttcctggc
     8881 gctgttcctg acagctgggc tgggtagtgg ttccactttc cagatgattt cagtgatctt
     8941 ccgtaaactg acaatggatc gcgtgaaagc agaagggggt tctgacgaac gtgcgatgcg
     9001 tgaagcggca accgacacgg cagcggcgct gggtttcatc tctgcgattg gcgctattgg
     9061 tggcttcttt atcccgaaag cgtttggtag ctcgctggca ttaacgggtt cgccagtcgg
     9121 cgcaatgaag gtatttttga ttttctatat cgcctgcgta gtg
//
LOCUS       DAZOZD010000164         9082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOZD010000164 DAZOZD010000000
VERSION     DAZOZD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.164"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            68..1666
                     /gene="rtcR"
                     /locus_tag="ACHUPB_003164"
                     /pseudo
     CDS             68..1666
                     /gene="rtcR"
                     /locus_tag="ACHUPB_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1648..2406)
                     /locus_tag="ACHUPB_003165"
     CDS             complement(1648..2406)
                     /locus_tag="ACHUPB_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0759907.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2423..3253)
                     /gene="glpG"
                     /locus_tag="ACHUPB_003166"
     CDS             complement(2423..3253)
                     /gene="glpG"
                     /locus_tag="ACHUPB_003166"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0759908.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3298..3624)
                     /gene="glpE"
                     /locus_tag="ACHUPB_003167"
     CDS             complement(3298..3624)
                     /gene="glpE"
                     /locus_tag="ACHUPB_003167"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0759909.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3814..5319
                     /gene="glpD"
                     /locus_tag="ACHUPB_003168"
     CDS             3814..5319
                     /gene="glpD"
                     /locus_tag="ACHUPB_003168"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0759910.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5371..5976)
                     /locus_tag="ACHUPB_003169"
     CDS             complement(5371..5976)
                     /locus_tag="ACHUPB_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759911.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5979..6746)
                     /locus_tag="ACHUPB_003170"
     CDS             complement(5979..6746)
                     /locus_tag="ACHUPB_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759912.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6749..7459)
                     /locus_tag="ACHUPB_003171"
     CDS             complement(6749..7459)
                     /locus_tag="ACHUPB_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759913.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7475..8980)
                     /locus_tag="ACHUPB_003172"
     CDS             complement(7475..8980)
                     /locus_tag="ACHUPB_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0759914.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2342 a         2344 c         2243 g         2153 t
ORIGIN      
        1 aaacggagat tttccacaaa accaattcac cgataatgaa atatcgtctt ttataaggat
       61 atctaagatg cgtaaaacag tggcttttgg ctttgtcggt accgtgctgg attatgccgg
      121 acgcggcagt cagcgctggt cgaaatggcg tccgacgctt tgtttatgcc agcaagaatc
      181 gttagtcatc gatcggctgg aattgttgca cgacactcgc tcgcgctcgc tattcgaaac
      241 gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa gtcgtgggcg ttgagattga
      301 actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt tcgcccgtgg
      361 ttacgagttt cagccagaaa aagaagacta tttaattcac atcaccaccg gcacccacgt
      421 cgcgcagatt tgctggtttc tgctggcgga agcgcgttac ctgcccgccc ggctgataca
      481 atcttcacct ccgcgcaaaa aagaacagcc gcgcggcgca ggtgaagtga cgattatcga
      541 tctcgattta agccgttata acgccatcgc cagccgcttt gccgaggaac gccagcaaac
      601 gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc gcatgattga
      661 gcagatcgaa aaagtggcga tcaaatcccg cgcgccgatt ctgcttaacg gtccaaccgg
      721 cgcgggcaag tcatttctgg cgcgacgcat cttcgagtta aaacaggcgc ggcatcagtt
      781 tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg ccatgtcgac
      841 gctgtttggt catgtaaaag gcgcgtttac cggggcgcgg taatctcgtg aaggtttatt
      901 acgcagcgcc aacggcggaa tgttgtttct tgatgagatt ggcgaactgg gcgcagacga
      961 acaggcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg gcagcgatcg
     1021 ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc gccagctggt
     1081 tgccgaaggc aaatttcgcg aagatctgta cgcgcggatc aatctctgga ccttcaccct
     1141 gccgggtcta cgccagcgcc aggaagatat tgaaccgaac cttgattatg aagtggagcg
     1201 ccacgccaca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc gcgcctggtt
     1261 ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac tttctgccag
     1321 cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg tggttgaaga
     1381 tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc ttacggcgtt
     1441 gctgggcgca gaggcagaaa acatcaatct cttcgaccgt atgcaactgg aacacgttat
     1501 cgctatctgc cgctaggcaa agtcgctttc tgcagccgga cgacagcttt ttgatgtttc
     1561 gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc tggcgcgttt
     1621 tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat gatggtccgt
     1681 cagcacctgc attacgctta ctggcggcgg ggcgtcggtg tagacggcat ctaccatgct
     1741 gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga caaccagcat
     1801 aacgtggcgc gagttctcaa taatggcgcg cttggtgcga acttcgtggt aatcgaactc
     1861 cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat caaggcggaa
     1921 ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc tgcgtaattc
     1981 gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag caacattgag
     2041 attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca ccgcttccgg
     2101 cgtggtgccg atatcgataa acagtgtcga gccgttgggg atttgctccg ccactttgcg
     2161 ggcaatcctc tctttttctt cggtctgggt ggctttgcga tcgtgccacg gcgtgttaac
     2221 cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt gctccgccag
     2281 ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta ccagctcttc
     2341 agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac gttgtgtttg
     2401 tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa tcaacaaaag
     2461 ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc gacatcccaa
     2521 acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac ccacgttgca
     2581 ggtaaatgcc actttgcgga tcgcgttcac cacgtagcca gacgtagccc atcagcgcat
     2641 acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc acatagccgc
     2701 ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg cgtttttcca
     2761 ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc agcgagaagt
     2821 gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgctgga tcgaatggcc
     2881 aggccagcca taacatcact tcctgatcgc cgagaatttg catggcaata aacaccacca
     2941 cgcaggcgat catcatcacc caggtcaccg gacctgcgcg ttcacgcaag gcggcaaaga
     3001 aaggataacg gcgataatgc aggccactgc cggtatggcc tgcctgccag ctggccgcca
     3061 gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgt acgcgctcgg
     3121 cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc gtgaggataa
     3181 caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta gcaaaagagg
     3241 taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta taaagcgtta
     3301 cgcgccgtac gccacctctg cgggaaactg acgttgccag gcttcaaagc cgccgtcaat
     3361 gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt tgctgctatt
     3421 gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca taaacgcgcc
     3481 cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgc cccatagcga aactctgtgg
     3541 atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg cgtcggcaac
     3601 gttaatacat tcgaactgat ccatgcgtct ctctttcttt acaaacaagt gggcaaattt
     3661 accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat atcactctaa
     3721 aatgtttttt caatgttacc taaagcgcga ttctttgcta atatgttcga taacgaacat
     3781 ttatgagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct gattgtgata
     3841 ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg tttatccgtg
     3901 ctgatgctgg aggcgcagga tctcgcttgc gcgacctctt ccgccagttc aaaactcatt
     3961 cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga ggcgctggct
     4021 gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccgatgcg ttttcgcctg
     4081 ccacatcgtc cgcatctgcg cccggcgtgg atgattcgca ttggtctgtt tatgtacgat
     4141 catctgggta aacgcaccag cctgccggga tcaactggtt tgcgttttgg cgcaaattca
     4201 gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt agacgacgcc
     4261 cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt gcttactcgg
     4321 actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc ggaagatatc
     4381 gataccggca aaaaatatag ctggcaagcg cgcggcttgg ttaacgccac cggcccgtgg
     4441 gtgaaacagt tcttcgacga caggatgcat ctgccttcgc cttatggcat tcgcctgatc
     4501 aaaggcagcc atattgtggt gccgcgcgtg catacccaaa agcaggccta cattctgcaa
     4561 aacgaagata aacgcattgt gttcgtgatc ccgtggatgg acgagttttc catcatcggc
     4621 accaccgatg tggaatacaa aggcgatccg aaagcggtga agattgaaga gagtgaaatc
     4681 aattacctgc tgaaagtgta taacacgcac tttaaaaagc agttaagccg tgacgatatc
     4741 gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc gccgcaggct
     4801 attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc gctgctgtcg
     4861 gtattcggcg gtaagttgac cacctaccgc aaactggcgg aacatgcgct ggaaaaactg
     4921 acgccgtatt atcagggcat tggcccggca tggacgaaag agagcgtgct gccgggtggt
     4981 gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta tccattcctg
     5041 actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga gctgctgctc
     5101 ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt ctacgaagcg
     5161 gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc cctttggcgt
     5221 cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag tcagtggctg
     5281 gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc ggttgaagtg
     5341 caaccgggct atttggattc cttagactat tcattgtaaa taattgaata acggcccctg
     5401 agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc tctggtttct
     5461 attttttctg caccaatggc aataatatcc caatgcaaaa ccaagctgtg cactaatcgg
     5521 gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg gaggccagac
     5581 aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg gagaaatata
     5641 aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca gccagcaatg
     5701 cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga tgccagccat
     5761 tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct gaagagaaat
     5821 acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta acgtgaatat
     5881 aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa caaaaccgag
     5941 aattacaggg ggaatataaa gccggaattt aatcattttt attttcctgg tgattcggat
     6001 gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc ccagacctga
     6061 tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg cgctctttct
     6121 ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat ggcgttatcg
     6181 atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa acaggcaaga
     6241 gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg cgcaagagcg
     6301 atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc atcgccggat
     6361 acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc atccgcacgc
     6421 cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg aatattgtca
     6481 aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac ctcatcgcta
     6541 ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata cgggatcgct
     6601 cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata ttcacaacaa
     6661 accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg ctcaattaat
     6721 gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg cgtgtaaacg
     6781 gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta cgcgaaaggg
     6841 aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg caattttcca
     6901 ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat ccgcgtaaac
     6961 tgttattgtt atgaaatttc caggcatctt taaagacctg aaagatatag acatcctcaa
     7021 tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca tccggattta
     7081 gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat tgaacatctg
     7141 gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa agacggctaa
     7201 tggtggcggt atcgagggaa aatcccggcc ataccttgct ggaggttaag ccaatattta
     7261 atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac ttttcccctt
     7321 catccaaggc ctggttaata agttgctgaa caatgccttc atcccagtat gccgatttgc
     7381 agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg cttgcaaata
     7441 atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta tacatcggga
     7501 cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac tccaacaatt
     7561 tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa ggcatcgacg atatagggat
     7621 atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct agcagtttta
     7681 tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct attgcgttgt
     7741 ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt gccacttctg
     7801 tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc accgtctggc
     7861 taccagagtc ttccgggcgt tgccaggtct ggatctcctg atcgttaccg ccaacagtac
     7921 gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc agggaattaa
     7981 ccggattatc cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc gtgtacagca
     8041 aagtaacgcc cgattcctca gcgcgttttt tctgcccacc ggaaggttgc gccacaaaaa
     8101 taatatcagc atcaccttta acaatcctgt tatacgcatc gggggtacga gagctctcca
     8161 gatattccct ggtgtgaaaa tcttccggta taacgcttaa agcataaaat gcagaggcat
     8221 aaattgggta cgccgccgta gcaccatcga gtcgcggcca gttttgcgta aactgaattt
     8281 gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc caggcccagg
     8341 tatcaatatt ttcgcgcaca ccgacaccgg gattgagctg tttatataaa taaccctgat
     8401 aaccagcgac aaaaccaagc atgagaagga taaacaggag ggaattacgg caacgtagcc
     8461 cggatgtacc ggttaaagga tgtctggttg gccggccgta ccccagcgaa aaactgatat
     8521 gcgcgccaac gggtataatg actatccacc agtactcacc tccgactact gagactaaca
     8581 aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgatcccatc tggtgaaatt
     8641 gataatctgc cagatgcagc gcgattatcc agccaaccag gattaataaa ccgggtacta
     8701 aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta tcgcctgccc
     8761 atactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa atgttgagca
     8821 gaaggtaact tgttggcgtg ataaggatat tacaaacttc aataataccg gcaagtccga
     8881 cacccagcat agcaacaacc gccgccaaaa attgcgtcag tatggggatg ccgaaaaaag
     8941 tcattaccag cgaggacaaa atccattttc tgttttgcat tattctttcc attctttttg
     9001 aatggtgaaa ttatactccc cgagtcccct tgccccttct ggacactttt ccgaaatgat
     9061 ggcggaaaaa aacgggaccc tt
//
LOCUS       DAZOZD010000165         8939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOZD010000165 DAZOZD010000000
VERSION     DAZOZD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.165"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            97..249
                     /gene="hokE"
                     /locus_tag="ACHUPB_003173"
     CDS             97..249
                     /gene="hokE"
                     /locus_tag="ACHUPB_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0759915.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(371..991)
                     /gene="entD"
                     /locus_tag="ACHUPB_003174"
     CDS             complement(371..991)
                     /gene="entD"
                     /locus_tag="ACHUPB_003174"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0759916.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1166..3406)
                     /gene="fepA"
                     /locus_tag="ACHUPB_003175"
     CDS             complement(1166..3406)
                     /gene="fepA"
                     /locus_tag="ACHUPB_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0759917.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3649..4851
                     /gene="fes"
                     /locus_tag="ACHUPB_003176"
     CDS             3649..4851
                     /gene="fes"
                     /locus_tag="ACHUPB_003176"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0759918.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4854..5072
                     /gene="ybdZ"
                     /locus_tag="ACHUPB_003177"
     CDS             4854..5072
                     /gene="ybdZ"
                     /locus_tag="ACHUPB_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0759919.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            5069..>8939
                     /gene="entF"
                     /locus_tag="ACHUPB_003178"
     CDS             5069..>8939
                     /gene="entF"
                     /locus_tag="ACHUPB_003178"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0759920.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRAT"
BASE COUNT         1923 a         2379 c         2466 g         2171 t
ORIGIN      
        1 aagccccagg agatatttct atcaatcctg gggctgccac tccgaacctc aacaactcgg
       61 atggtagtcc cttcttcgca tggaggcaat ataaacatgc tgacgaaata tgcccttgtg
      121 gcagtcatag tgctgtgttt gacggtgctg ggatttacgc ttctggtcgg agactcgctg
      181 tgtgagttca ccgtaaagga acgtaatatt gagtttaagg ctgttctcgc ttacgaaccg
      241 aagaagtagc cgttgtgcgg ggagcaatcc ctgcatatcc ggttgtcagg tcagagtggt
      301 aaggcacctg ctttacactt tcgcccgcag tcagtgatgg ctgcgggcga atcgtaccag
      361 atgttgtcaa ttaatcgtgt tggcacagag ttatgactat tttttctttt atctgccagt
      421 gcacagcaaa catctcattc tcacgatgaa tgatgacctg ctgtttattc cagctaatta
      481 tctgataatc cagaaaacct gcttcagttt gtctctccga tgccttaaat gcgctctctt
      541 tggcggaaaa tgccagtgtc agcgccagag aaaaggctaa accacagtcc gcgagtcgct
      601 cgtgttctgc tggtgtaata atattgtctg tcaattctct tgcggtttgt acagaaaaaa
      661 tttcttctat atcaatgcca atcggttgac gagataccac ggctaatgcc gtagtcccac
      721 agtggctaat actgccgtat acctccgcag gccagacagg ttggcgtagc tcgccgattg
      781 cgggcacaca tttatagcca tattcccgta aggcataaac agcagcgatc cgtccggcta
      841 aatgctctgt tttacgttta cgtccagcgt gttgcaattg tgcgtagtgc ggcagccaga
      901 gtaaatcctg ctcacaaaaa ttcgccggat cgaactcaac aaaatgcagc gtatgtccgg
      961 caaaggggag ggaggtatgc gtagttttca tatcgaccat attcgagact gatgacaaac
     1021 gcaaaactgc ctgatgcgct acgcttatca ggcctacatg gtcctgcaat atattgaatt
     1081 ggcaagattt ttgtaggccg gataaggcgt tcacgccgca tccggcatga acgacgcgca
     1141 ctttgtcaac aatctgacgt tagcatcaga agtgagtgtt tacgctcatg taccacgtac
     1201 gtcccggctc gttataggta tacgccccgg cacccgcgat atagttggcc cctgccagat
     1261 cgcccgtggt ctgggcatta cccgcacgcc acaaacgttt gtcgaacagg ttgtccacgc
     1321 cgccggtcag actgacattc ttcgtcacat cccaggtcgc gctcagaccc acaatgctgt
     1381 aaggactgat ttctttggtt tccgggccaa ccgctggctg acctttgtag ttgtattttt
     1441 tcggctgctg cttaccgtac caggtgaagg tcgtttgcat cgacaaatct tcccgcgcct
     1501 gccagctcag cgttgagttc aacgtgtact ccgggatgat cgacaaacgg tcgcccgtgg
     1561 ttttgttttc actcttcagc atataagtga tgttattggt ccacatcacc atttcgctaa
     1621 ctggtacgtt taacgatcct tccagacctt caaccactgc tttcggcacg ttatcccact
     1681 gatagagatc ggtgccgact gcgttttgcc ctacagccac atagcccgct tcaatcttat
     1741 tgcgataatc gttacggaac caggtgacgc ccgccagcca gccgtcgcgt ttgaactcca
     1801 gaccaatctc tttgttgatg ctggtttctg ctttcaggtc atcgttacct tgctgatagc
     1861 agccgcccgc gctggcatag cagccctgac ctttactgta gagaatgtag ttcgggttag
     1921 tctggtacag gctcggtgct ttataagcac gggcaatgcc cattttgagc gtgaagtcat
     1981 cgcctaaacc ttgcgatatg ttcagcgccg ggctccagtt attgccgaca atactgtgat
     2041 gatcgaaacg cagccccggc gttacgatgg tgctgtcagt cagctccatg ttgttttcgg
     2101 caaacagcga gaaaatttct gcttttgaat acgggctacg gtcggtggca ctcacaccat
     2161 caatagcgcc gccggtgttc gccccggtca gcgcctgggt attggaactc aagtccttca
     2221 tccgttgctg attccactcc gtacccagcg tcagcgtttg gttaacgagg aaatcaatcg
     2281 gcaggttaac ttcgctgtgc agcatcacgt catcaagatc gatatcgacg aaatcctgtg
     2341 tcgctttttc gttaaatttc ccttcggtac cgcctgccag accttccgga atacgcgagt
     2401 tacgggtgtg ttcatactgc acccagttgc tggtggtcac tccgttatcc cagccgccgt
     2461 tccaggtcag cgagtagttc tggcgataca gacggttggt ttcatcgcca tatttcgagc
     2521 gggtatagga atcggagttg gtgttctggg tgtcgcccgc atacaggttg ccctggcggc
     2581 tgtaacctgc ttccagttcc agcgattgca atggcgcgaa atcccagcgc accacgccat
     2641 taatatcttt gttgatcacc ccttcgcgcc cggctggtaa cgtcgtggca tacgttccgg
     2701 cacgcgcgga ctgatggccc tggttgatat cccacgcgtc agcctgggtt ttgtcgaggt
     2761 tgccatacaa gcggaagctg aattcgtcgc ccagcggacc ggtcaggctg aagttggtgc
     2821 gtttagtggc accttcctct ttatgttctg gtgcattgaa ataagcgtcc caggagccgt
     2881 gccactcacc gctgcctttt ttggtaatga tgttaaccac gccgcccgcc gcgccgttgc
     2941 cataacgcgc agctgccgga ccacgcagaa cttcaatacg ttcaatcatt tcaggtggca
     3001 cccaggaggt atcaccacgg gtatcgcgct cgccacgcca gccctgacgc accgagttac
     3061 ggctgcttac cggcttgccg tcaatcaaaa tcagcgtgtt ttccggaccc ataccgcgaa
     3121 tatcaatctg tcggttattc ccacgctgac cactggtgga gttaccggtc aggttaacgc
     3181 ctggcatggt acggatgatc tccgacacat cgcgggcaac ggggtttttg cggatttcat
     3241 ctgcggtgat ggtcgaaacg ccaggcgcct gtaagttctg ctcggcggcg gtaacgacaa
     3301 tggtatcgtc atgtgaaaca ggagtatcgg tcggctcttg tgcctgcgct accccataaa
     3361 tccccagatt gaccaacaag gccagggaat gaatcttctt gttcattgtt ttattcctgc
     3421 atttttgcca cgaattgcaa ctgtcgggca tggtcgtcct caacacgacg catcccgcta
     3481 ccgcgaaaac ctttgatcct gaaagacacg cagtgcagtt ggttaataaa tgtccgcgct
     3541 tcccacggcg cgccattacg ctattgcaaa tgcaaatggt tatcaataat attatcaata
     3601 tatttctgca atcaatgaaa aattgcacag taaacatggg gttatggtgt gacggcgtta
     3661 aaagtaggaa gtgagagctg gtggcagtcg aaacatggcc cggaatggca gcgtctgaat
     3721 gacgaaatgt ttgaggtcac tttctggtgg cgtgatcccc aaggttctga agaatactcg
     3781 acgataaagc gcgtatggat ctacatcact ggtgtgaccg atcatcatca gaacagccag
     3841 ccccagtcga tgcagcgaat tgcaggtact gatgtctggc agtggacgac acaactcaat
     3901 gccaactggc gcggcagcta ctgctttatt cccaccgaac gcgatgacat tttttctgca
     3961 ccatcccccg atcgcctcga attacgtgaa ggctggcgaa aactattacc ccaggcaata
     4021 gccgatccgc tgaatccaca aagctggaaa ggcgggcgag ggcacgctgt ttctgcactc
     4081 gaaatgccgc aagcgcctct gcaaccggga tgggattgtc cgcaagtgcc agaaatagca
     4141 gccaaagaaa ttatctggaa aagtgaacgg ttgaaaaatt cacgtcgtgt atggattttt
     4201 accaccggcg atgtaacagc agaagaacgt ccgctggcag ttttgctcga tggcgaattt
     4261 tgggcgcaaa gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt
     4321 cctcccgccg tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc ccacgaactg
     4381 ccgtgtaatg cggatttctg gctggcagta cagcaagagt tattacccct ggtgaaagct
     4441 attgcccctt ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag ttttggtggg
     4501 ctgtccgcgc tgtatgccgg actgcactgg cctgaacgct ttggctgtgt attaagccag
     4561 tcaggatcgt actggtggcc gcatcgggac gggcagcaag agggcgtgtt acttgaaaag
     4621 ctaaaagctg gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc
     4681 gagccgatga tcatgcgggc caatcaggcg ctgtatgcgc aattgcaccc tataaaagaa
     4741 tccattttct ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg cggtggcttg
     4801 atgcaggggt taatcgacct ctggcaacca cttttccatg acaggagttg agtatggcat
     4861 tcagtaatcc cttcgatgat ccgcagggag cgttttacat tttgcgcaat gcgcaggggc
     4921 aattcagtct gtggccgcaa caatgcctct tatcggcagg ctgggacatt gtgtgtcagc
     4981 cgcagtcaca ggcgtcctgc cagcagtggc tggaagtcca ctggcgtact ctgacaccgg
     5041 ccaatttcac ccagttgcag gaggcacaat gagccagcat ttacctttgg tcgccgcaca
     5101 gcccggcatc tggatggcag aaaaactgtc ggatttaccc tccgcctgga gcgtggcgca
     5161 ttacgttgag ttaactggag atgttgatgc gccattactg gcccgcgcgg tggttgccgg
     5221 actagcgcaa gcagatacgt tgcggatgcg ttttacggaa gataacggcg aagtctggca
     5281 gtgggtcgat gatgcgctga tattcgaaca gccagaaatt atcgacctgc gaaccaatat
     5341 tgatccgcac ggtactgcgc aggcattaat gctggcggat ttgcaacaag atttgcgcgt
     5401 cgatagcggt aaaccactgg tctttcatca gctgatacat gttgcggata accgctggta
     5461 ctggtatcag cgttatcacc atttgctggt cgatggcttc agtttcccgg ccattactcg
     5521 ccagatcgcc aatatttact gcacatggct gcgtggcgaa ccaacgcctg cttcgccgtt
     5581 tacgcctttc gctgatgtag tggaagagta ccagcaatac cgcgaaagcg aagcctggca
     5641 gcgtgatgcg gcattctggg cagaacagcg tcgtcaactg ccgccgcccg cgtcactttc
     5701 tccggcacct ttaccggggc gcagcgcttc ggcagatatt ctgcgcctga aactggaatt
     5761 taccgacggg gaattccgcc agctggctac gcaactttca ggtgtgcagc gtaccgattt
     5821 agcccttgcg ctggcagcct tgtggctggg gcgattgtgc aatcgcatgg actatgccgc
     5881 cggatttatc tttatgcgtc gactgggctc ggcggcgctg acggctagcg gacccgtgct
     5941 caacgtttta ccgttgggta ttcacattgc ggcgcaagaa acgctgccgg aactggcaac
     6001 ccgactggcg gctcaactga aaaaaatgcg tcgccatcaa cgttacgatg ccgaacaaat
     6061 tgtccgtgac agcgggcgag cggcaggtga tgaaccgctg tttggtccgg tactcaatat
     6121 taaggtattt gattaccaac tggatattcc tggtgttcag gcgcaaaccc ataccctggc
     6181 aaccggtccg gttaatgacc ttgaactggc cctgttcccg gatgaacacg gtgatttgag
     6241 tattgagatc ctcgccaata aacagcgtta cgatgagcca acgttaatcc agcatgctga
     6301 acgcctgaaa atgctgattg cccagtttgc cgcggatccg gcgctgttgt gcggcgatgt
     6361 cgatattatg ctgccaggtg agtatgcgca gctggcgcag atcaacgcca ctcaggttga
     6421 gattccagaa accacgctga gcgcgctggt ggcagaacaa gcggcaaaaa caccggatgc
     6481 tccggcgctg gcagatgcgc gttacctgtt cagctatcgg gaaatgcgcg agcaggtggt
     6541 ggcgctggcg aatctgctgc gtgagcgcgg cgttaaacca ggggacagcg tggcggtggc
     6601 actaccgcgc tcggtctttt tgaccctggc actccatgca atagttgaag ctggcgcggc
     6661 ctggctaccg ctggataccg gctatccgga cgatcgcctg aaaatgatgc tggaagatgc
     6721 gcgtccgtcg ctgttaatta ccaccgacga tcaactgccg cgctttagcg atgttcccaa
     6781 tttaacaagc ctttgctata acgccccgct taccccccag ggcagtgcgc cgctgcaact
     6841 ttcacaaccg caccacacgg cttatatcat ctttacctct ggctccaccg gcaggccgaa
     6901 aggggtaatg gtcgggcaga cggctatcgt taaccgcctg ttatggatgc aaaatcacta
     6961 cccgcttaca ggcgaagatg tcgttgccca aaaaacgccg tgcagttttg atgtctcggt
     7021 gtgggagttt ttctggccgt ttatcgcagg ggcaaaactg gtgatggctg aaccggaagc
     7081 gcaccgtgac ccgctcgcta tgcagcaatt ctttgccgaa tatggcgtaa cgaccacgca
     7141 ctttgtgccg tcgatgctgg cggcatttgt tgcctcgctg actccgcaaa ccgctcgcca
     7201 gagttgcgcg acgttgaaac aggttttctg tagtggtgaa gccttaccgg ctgatttatg
     7261 ccgtgaatgg caacagttaa ccggtgcgcc gttacataat ctgtatggcc cgacggaagc
     7321 ggcggtagat gtcagctggt atccggcttt tggcgaggaa ctggcacagg tgcgcggcag
     7381 cagtgtgccg attggttatc cggtgtggaa catggggctg cgtattcttg atgcgatgat
     7441 gcatccggtg ccgccgggtg tggcgggtga tctctatctt accggcattc aactggcgca
     7501 gggctatctc ggacgccccg atctgaccgc cagccgcttt attgccgatc cttttgctcc
     7561 tggcgaacgg atgtaccgta ccggagacgt tgcccgctgg ctggataacg gcgcggtgga
     7621 gtacctcggg cgcagtgacg atcagctaaa aattcgcggg cagcgtatcg aactgggcga
     7681 aatcgatcgc gtgatgcagg cgctgccgga tgtcgaacaa gccgttaccc acgcctgtgt
     7741 gattaaccag gcggcagcca ctggtggtga tgcgcgtcag ttggtgggct atctggtgtc
     7801 gcaatcgggc ctgccgttgg ataccagcgc attgcaggcg cagcttcgtg aaacattgcc
     7861 accacatatg gtaccggtgg ttctgctgca acttccacag ttacctctta gcgccaacgg
     7921 caagctggat cgcaaagcct taccgttgcc tgaactgaag acacaagcgt cgggacgtgc
     7981 accgaaagcg ggcagtgaaa cgattatcgc cgcggcattc gcgtcgttgc tgggttgtga
     8041 cgtgcaggat gccgatgctg atttctttgc gctgggcggt cattcgctac tggcaatgaa
     8101 actggcagcg cagttaagtc gacagtttgc tcgtcaggtg acgccggggc aggtgatggt
     8161 cgcgtcaacc gtcgccaaac tggcaacgat tattgatggt gaagaggaca gctcccggcg
     8221 catgggattc gaaaccattc tgccgttgcg tgaaggtaat ggcccgacgc tgttttgttt
     8281 ccatccggcg tccggttttg cctggcagtt tagcgtactc tcgcgttatc tcgatccaca
     8341 atggtcgatt attggcattc agtcgccgcg ccctcatgga cccatgcaga cagcgacaaa
     8401 cctggatgaa gtctgcgaag cgcatctggc aacgttactt gagcaacaac cgcacggccc
     8461 ttattacctg ctggggtatt cccttggcgg tacgctggcg cagggtattg cggcgcgact
     8521 gcgtgcccgt ggcgaacagg tggcatttct tggcttgctg gatacctggc cgccagaaac
     8581 gcaaaactgg caggaaaaag aagctaatgg tctggacccg gaagtgctgg cggagattaa
     8641 ccgcgagcgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg agttgtttac
     8701 caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc atagcgtacc
     8761 gtttgacggt aaagcgacgt tgtttgttgc tgaacgcacg cttcaggaag gtatgagtcc
     8821 tgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg attgtgcgca
     8881 tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc gcgcaacgc
//
LOCUS       DAZOZD010000166         8878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZOZD010000166 DAZOZD010000000
VERSION     DAZOZD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.166"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            13..303
                     /gene="yebG"
                     /locus_tag="ACHUPB_003179"
     CDS             13..303
                     /gene="yebG"
                     /locus_tag="ACHUPB_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR0759921.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            370..726
                     /gene="yebF"
                     /locus_tag="ACHUPB_003180"
     CDS             370..726
                     /gene="yebF"
                     /locus_tag="ACHUPB_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0759922.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            1053..1712
                     /locus_tag="ACHUPB_003181"
     CDS             1053..1712
                     /locus_tag="ACHUPB_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0759923.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            1921..3981
                     /gene="ptrB"
                     /locus_tag="ACHUPB_003182"
     CDS             1921..3981
                     /gene="ptrB"
                     /locus_tag="ACHUPB_003182"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0759924.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(3978..4640)
                     /gene="exoX"
                     /locus_tag="ACHUPB_003183"
     CDS             complement(3978..4640)
                     /gene="exoX"
                     /locus_tag="ACHUPB_003183"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0759925.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4664..5320)
                     /gene="yobB"
                     /locus_tag="ACHUPB_003184"
     CDS             complement(4664..5320)
                     /gene="yobB"
                     /locus_tag="ACHUPB_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR0759926.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(5422..5652)
                     /gene="holE"
                     /locus_tag="ACHUPB_003185"
     CDS             complement(5422..5652)
                     /gene="holE"
                     /locus_tag="ACHUPB_003185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0759927.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            5791..6165
                     /gene="yobA"
                     /locus_tag="ACHUPB_003186"
     CDS             5791..6165
                     /gene="yobA"
                     /locus_tag="ACHUPB_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0759928.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            6169..7041
                     /gene="copD"
                     /locus_tag="ACHUPB_003187"
     CDS             6169..7041
                     /gene="copD"
                     /locus_tag="ACHUPB_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR0759929.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            7054..7395
                     /locus_tag="ACHUPB_003188"
     CDS             7054..7395
                     /locus_tag="ACHUPB_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0759930.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(7788..8864)
                     /locus_tag="ACHUPB_003189"
     CDS             complement(7788..8864)
                     /locus_tag="ACHUPB_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0759931.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
BASE COUNT         2242 a         2119 c         2184 g         2333 t
ORIGIN      
        1 gagaggctgc aaatggcggt tgaagttaaa tacgtagtca ttcgtgaggg cgaagagaaa
       61 atgtcgttta ccagcaaaaa ggaagccgat gcttatgaca aaatgcttga tacggcggat
      121 ctgcttgaca cctggctgac aaattctcca gtgcaaatgg aagacgagca acgtgaagcc
      181 ctttcgctat ggctggcaga acaaaaagat gtgctgagca ccattctgaa aaccggcaaa
      241 ttaccgtccc cacaggtcgt cggtgctgaa tccgaagagg aagatgcctc acacgcggcc
      301 tgaagcaact tgcgctcctg acaatttgta ccatgctttc tcctgaatca ttgtgctatg
      361 gagaaaaaca tgaaaaaaag aggggcgttt ttagggctgt tgttggtttc tgcctgcgca
      421 tcagttttcg ctgccaataa tgaaaccagc aagtcggtca ctttcccaaa gtgtgaaggt
      481 ctggatgctg ccggaattgc cgcgagcgta aaacgtgatt atcaacaaaa tcgcgtggcg
      541 cgttgggcag atgatcaaaa aattgtcggt caggccgatc ccgtggcttg ggtcagtttg
      601 caggacattc agggtaaaga tgataaatgg tcagtaccgc taaccgtgcg tggtaaaagt
      661 gccgatattc attaccaggt cagcgtggac tgcaaagcgg gaatggcgga atatcagcgg
      721 cgttaatggc ttctgataat tcgattgacc gttggtggat gcggcgcgag tgccttgtcc
      781 gccccagaac gcaccacaat cgtaggcctg agaagacgca acagcgtcgc atcgggcaat
      841 cagcatcaaa caatgccact caaaacgaca aactactttc acctaaaata cccttatcac
      901 ctgatataca gtaaatacct tacctggcgc gaatttcttt gaataacggc ctaccgcccc
      961 gcttatttgc gcatcttttg tctcgatata cattttctga catcctcatc cgctatgcta
     1021 caggcactaa aaatggttat tgaggagcaa atatggctaa ctggttaaat caactgcaat
     1081 ccctgcttgg gcaaagcagt tcttctacct cctcgtctgc ggatcaggga ttgggcaaac
     1141 tgttagtgcc aggcgcatta ggcggactgg ctgggctgct ggtcgcaaat aaatcagcac
     1201 gtaaacttct tacgaaatat ggcaccaacg cgttactggt tggcggcgga gcggtagcgg
     1261 gtacggtgct gtggaataaa tacaaagata aaattcgcgc ggcgcatcag gacgaaccgc
     1321 agtttggcgc gcaaagtacg ccgctggatg agcgtacaga acgtttgatc cttgcgctgg
     1381 tctttgccgc taaaagtgat ggtcatattg atgccaaaga acgtgcggca atcgaccagc
     1441 aattgcgtga agccggcgtg gaagagcagg ggcgtgtact cattgagcag gcaatcgaac
     1501 aaccgctgga tccacaacgc ctggctaccg gggtccgcaa tgaagaagag gcgctggaaa
     1561 tctatttcct gagttgcgcg gctattgata ttgaccattt tatggaacgc agttacctga
     1621 atgcactggg tgacgcgctg aaaatccccc aggatgttcg tgacggcatt gaacgcgatc
     1681 tcgaacagca aaaacgcact ttggcggaat aattccgctc tctggatagc ctgaagttga
     1741 ctatttcggg ctatccagaa ccgcacagcc atttgaacag gctgagtttc tctgtctgca
     1801 taacattcga tatccgtgcc attacctgtc atcatctaag caatgactcc cctgtttcgc
     1861 ttgcatcccc ggtgagtttt gccaccctta taagatgttt caaccagaaa gaacaataac
     1921 atgctaccaa aagccgcccg cattccccac gccatgacgc ttcatggcga tacgcgcatc
     1981 gataattact actggctgcg ggatgatacg cgttctcagc cagaagtcct ggactacctg
     2041 caacaagaaa atagttacgg tcatcgggtg atggcctcac aacaagcctt gcaggatcgc
     2101 atcttaaagg aaatcatcga ccgcattccg caacgagaag tttctgcgcc ctacatcaaa
     2161 aatggctacc gctatcggca tatttatgaa ccaggctgtg aatatgctat ctaccagcgt
     2221 caatcggcat tcagtgaaga gtgggatgag tgggaaatat tgctcgatgc caataagcgc
     2281 gcagctcata gtgagtttta ttcgatgggc ggaatggcga ttacgcccga taacaccatt
     2341 atggcgctgg cagaagattt tctttcccga cgccagtacg gcattcgttt tcgtaatctg
     2401 gaaactggta actggtaccc ggaactgctg gataacgttg aacccagctt tgtctgggca
     2461 aatgactcct ggactttcta ctatgttcgc aagcatccgg tgacgctgct gccttatcag
     2521 gtctggcgtc acgccatcgg tacgccagca tcgcaagata aactgatcta cgaagaaaaa
     2581 gacgatacct attacgtcag cctgcataaa acgacctcga agcactatgt agtcattcat
     2641 ttggccagcg ccaccaccag tgaagttcgc ctgctggacg cggaaatggc cgatgccgag
     2701 ccgtttgttt ttctgccgcg ccgcaaagat cacgaataca gccttgatca ctaccagcat
     2761 cgtttttatc tgcgttccaa ccgccacggc aaaaactttg gcttataccg tacccgtatg
     2821 cgtgatgagc aacagtggga agagttaatt ccgccatgcg aaaacatcat gctggaaggg
     2881 tttacgctgt ttaccgactg gctggtggtt gaagagcgtc agcgcgggtt aaccagtttg
     2941 cgccaaatta accgcaagac ccgggaagtc attggtattg cctttgatga tccagcctat
     3001 gtgacctgga ttgcctacaa tccagaacct gaaaccgcgc gattgcgtta tggttattct
     3061 tccatgacca caccagacac tttgtttgaa ctggatatgg ataccgatga gcgtcgtgta
     3121 ttaaaacaaa cggaagttcc tggttttgat gcggcgaatt accgcagtga acacctgtgg
     3181 atagtcgccc gtgatggcgt cgaagttccg gtttcgttgg tctaccatcg caaacatttt
     3241 cgcaaaggac acaacccgtt gctggtgtat ggctatggtt cttacggcgc aagtattgat
     3301 gccgatttca gttttagccg cttgagtttg ttagatcgtg gctttgtcta cgccattgtc
     3361 catgttcgcg gcggtggtga gctggggcaa caatggtacg aagacggaaa atttctgaag
     3421 aagaaaaata cgtttaatga ttatcttgat gcctgcgatg cattgttaaa actgggctat
     3481 ggctctcctt cgctttgtta tgcgatgggc gggagtgcgg ggggcatgtt gatgggcgtt
     3541 gcaattaatc aacgcccgga attattccac ggcgttatcg cccaggtacc gtttgttgat
     3601 gttgtaacaa cgatgcttga tgaatcaatt cctcttacca ctggtgagtt tgaagagtgg
     3661 ggtaacccgc aggatccgca atattacgag tacatgaaaa gctacagccc gtatgacaac
     3721 gtcaccgcac aggcttatcc gcatttactg gtaacgaccg gtttacacga ttctcaggtg
     3781 caatattggg aaccggcaaa atgggtcgct aaattgcgcg agctgaaaac cgataaccat
     3841 cttttattgc tctgtaccga catggactca ggccatggcg gtaaatctgg ccgctttaaa
     3901 tcgtacgaag gcgtagcgat ggaatatgct tttctggtcg cgctggcgca gggaacatta
     3961 cccgctacgc ctgcggatta agtattttcc agataatgtt tcagtgttaa acgcagctcc
     4021 gggctcatgc tgtccaggtt attaaataac cagcgcagat agcccggatc gcgttcggca
     4081 acgtcggaaa ccgctttgcc acggtatttg ccaaaggtga aggtcgtcat caacgacgga
     4141 cgtccggtga tatcggccat ctgttctgcc gtccagccgg aggtgttcat aatatcgata
     4201 agcaacgcgg cggtgatata acagtcatac aacgcgcggt gatgatgcag gcccggcggc
     4261 gtctgtacat tgagcttccg tgttttatat aacgccatat tgctgtactt gattcctggc
     4321 cacaagcgac gggccagttt catggtgcaa atccactcac cgggcatctc aggcagtacg
     4381 cggcggtcaa agctggcgtt gtgcgcgaca taccattcac tgccgtaata gtgtgggatc
     4441 acatcttcaa tccacggttt atcggcgacc atggcttcgg tgatgcgatg aatcgccatc
     4501 gcttgtggac taataggacg atcggggcgc accaggtggc tcatggggtt gacgattttt
     4561 ccgtcaatga catcaacaga ggcaatctca acgatccctc cctgcaaacc gcaggtttct
     4621 gtatcgataa tgcgcaacat ggcatactcc aggccgaaaa agcctagcgt aatggaatga
     4681 tatcgccttg ccaaccctgt gatgaacgct gaccgactaa caataatgaa ccacgatcgg
     4741 cgcgaacgat gagacgacca tattcatccc atagtgcact gttaccacgg gcatttgcca
     4801 tcagcacagc aatcgagtac tgatgtgaaa aaaattgtaa gcgacgggcc gacgccagca
     4861 ggtcgggttc tcccagacat tgccctgtcg taaacagtga acaggtcggg tccatatcca
     4921 taccttccgg ttgttcatcg accaccgtga tggttctgga acgacggccc agacatgcac
     4981 catgactctg atggtagatc ccaggcgttt ttcgccacgg tgcgaacacc gcaattccac
     5041 gaataaagcg atcgttatat tcaacgggaa ggccggcaat gatggtcatt cgccaggtcg
     5101 ttgcggcata acaaagcggg tctaacagtg agagatcggg tggggcagga agggcacgtc
     5161 gcgaataatc acaccccagt aaagaaagcg atggaaaaac taacagctga cattgttgcc
     5221 tggcggcggc cctcacaaac tccagatgat gagctacttg ttcggtgagt gaggttttac
     5281 gaggctcata ttgcgctgct gcaactttcc agaacgacat aatgacttcc ttttcaaaat
     5341 gactgcaaat tatgggtgcg gtttagaaaa tactcataat ttagcaggcg ttatgtaaga
     5401 aagtgtaacc ctgtataagt tttatttaag tttgggctcg taaggtaaac gtgacagatt
     5461 gaccgaagcc aaacggtggg caataagccg ctcgcgaaac cagctgcgca aatgttcagg
     5521 ctgttcacgt tcaaccgctt cagcgatcac cggcatattg tagcgttctt taaatgccac
     5581 cccggccgcc gccaaatcga cattcacttt atccatttct gtttgatcca gtttagccag
     5641 attcttcagc attcttaatc tcctcatcat tcgcgggcaa aagttactaa gagtgagcca
     5701 ggatggcaag ccggaattga tgatcctcga ctcagacatt agacaaggtt atgctctgta
     5761 aaaaagttat tacaaaaagg aatagataat atggcttcaa ctgcacgctc ccttcgctac
     5821 gcgctggcaa tcctgaccac ttcgttggtg acacctttcg tctgggcaca tgcgcattta
     5881 acgcatcagt atcctgcggc aaacgcgcaa gtgacagctg caccgcaggc aatcacttta
     5941 aacttctcgg aaggtgtgga aacaggattc agtggtgcaa aaatcacggg gccaaaaaac
     6001 gaaaatatta aaacattacc ggcgaagcga aatgagcagg accaaaagca actgattgtt
     6061 ccactggccg attcgctgaa acccggaacc tacaccgttg actggcatgt tgtttcggtg
     6121 gacgggcata aaacgaaagg acactacacc tttagcgtga aataaatcat gctggcgttt
     6181 acctggatcg cgctgcgatt tatccatttc acctcgctga tgctggtttt tggcttcgca
     6241 atgtacggtg cctggctggc ccccttgacg attcgtcgct tgctggcgaa gcgttttctg
     6301 cgtttacaac agcacgctgc cgtctggagt ttgatcagtg ccactgcaat gctcgccgtt
     6361 cagggcgggt tgatgggaac gggatggaca gatgtatttt cgccaaacat ttggcaggct
     6421 gttttgcaaa cgcagtttgg tggcgtctgg ttatggcaaa ttgttctcgc cctcgtaacg
     6481 ttgatcgtcg cccttatgca accgcggaat atgccacgtt tgctgtttat gctcactacc
     6541 gcgcaattta ttctgctggc gggaatgggg catgcgacac tgaatgaagg ggtaacggcg
     6601 aaaatccatc agactaatca cgcgattcac ctgatttgtg cggcggcgtg gtttggggga
     6661 ttactgccgg tgctctggtg tatgcaactc atcaaaggcc gttggcgaca ccaggctatt
     6721 caggcgctga tgtgtttttc atggtgcggg cattttgcgg tgatcggcgt actggcaagc
     6781 ggcgtgctta atgcattgtt gattaccgga tttcccccca cgcttacgac ttactggggc
     6841 caactcttat tgctcaaagc catcctggtg atgattatgg tggtcattgc gctggctaat
     6901 cgttatgttc ttgtaccgcg catgaggcag gaggaagatc gtgcggcacc ctggtttgtg
     6961 tggatgacta aactcgaatg ggcgataggt gcggtagtgc tggtgatcat cagcctgctt
     7021 gcgactctcg aacctttttg atggattacg aaaatgatga aaaaaagtat tctggcgttt
     7081 ctgttactca ccagttctgc agcggcgctg gctgcaccgc aggtgattac cgtcagtcgt
     7141 tttgaagtgg gtaaagacaa atgggcgttt aatcgcgaag aggtgatgct gacttgccga
     7201 ccgggtaatg ctttgtatgt tattaatccg agtaccctcg tgcagtatcc tttaaacgat
     7261 atcgcacaaa aggaagttgc cagtgggaag accaatgccc aacccatttc ggtgattcag
     7321 attgatgatc ctaacaatcc cggcgaaaaa atgagtctgg caccgtttat agaacgagct
     7381 gaaaaactct gttaattacc taaaatagcc ttttgatttc caataaaaaa accgcctcag
     7441 ttctttcacc agaacgggcg gtttttaaca tttaagctga tgaccaccgc gctttttatt
     7501 gaccattttg cacgcaaact ggaaaacctg gcgtcgtcat ctattcttaa agggcaaggc
     7561 aactaagcct gcattaatgc caacttttag cgcacggctc tctcccaaga gccatttccc
     7621 tggaccgaat acaggaatcg tattcggtct ttttttaatt gcatttgaaa tcaattagtt
     7681 gcaaatgtct cccccgaaat tccccgaaat ttactcgaat ttctgtattc cggtcttttt
     7741 ggttatatca caaccaaaat acatttaaca atccatttac gttaaaatca gagcagtaag
     7801 tacgtttttt ctctctcatc aagatacatt tttgttgtct tctccgatgt gtggccaagt
     7861 agacgctgag caaattcttc tccacatgtt tctttgtaca atcgtccagc caggcttctg
     7921 atctcgtgaa aagttggtgg gttttcactg aactggatac ctgtgaattt tctggctgcg
     7981 acaaattttt ttgtcaggcc atccgggtga atgctgccgt cagggctgtt ttttctaatc
     8041 ccggcactga ttagataatc tccccggctt accaggcggc atcgttcaac tactgtacca
     8101 agccgtagac cagcgacagg aaggctgagt gacaggggga tagcaatcat cattcctgtc
     8161 ttaatttgcc tgacgtggag acgatcatca taaatatcac tgaaccgcat attcgttatg
     8221 tcttcgcgac gttgtcctgt tacaagggct aaatccatag ctaatgggaa ccatgccgga
     8281 agttgatctg ctgcctccct gatgcagttg tatatcttta gtttcagtcg ttctcttgta
     8341 actactattt tcggtgctct tgttggcgtg actggatttt gagatatacg tccttcaaca
     8401 atggcctcgc gaaacatatc agacaacaca gaacgcattg atcctgccat tgtgtttttc
     8461 cctccttcaa tccacaaatc aagaaactcg gcaatatggc gagtggttat ttctgtcagt
     8521 aaaatctctc ccaatttttc ttttattgtc tccagttgat ttgcccgaat tttataagta
     8581 tttctggaca cttttctcct tataagaatc gttttgtaac gttcaatcca gtctgccata
     8641 gtaaatgagt cgaacccttt aagtttttca attaaggcag cgggagagta gtttttgtat
     8701 atataatgat ttgcttcaat tgcctgcgtt actgcatctc ttcttgaaat tttacctagt
     8761 gtaaattctt ctttcgtcag agggttgcgc cagtaatatg ctttgtctct ccttcgatat
     8821 gttaagtttc taggcaaatt gggatcgtat tttttccgct gcatgtttta acttctcc
//
LOCUS       DAZOZD010000167         8747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOZD010000167 DAZOZD010000000
VERSION     DAZOZD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.167"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            20..1744
                     /gene="iucA"
                     /locus_tag="ACHUPB_003190"
     CDS             20..1744
                     /gene="iucA"
                     /locus_tag="ACHUPB_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0759932.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1745..2692
                     /gene="iucB"
                     /locus_tag="ACHUPB_003191"
     CDS             1745..2692
                     /gene="iucB"
                     /locus_tag="ACHUPB_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0759933.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2692..4434
                     /gene="iucC"
                     /locus_tag="ACHUPB_003192"
     CDS             2692..4434
                     /gene="iucC"
                     /locus_tag="ACHUPB_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0759934.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4431..5768
                     /gene="iucD"
                     /locus_tag="ACHUPB_003193"
     CDS             4431..5768
                     /gene="iucD"
                     /locus_tag="ACHUPB_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0759935.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            5774..7969
                     /gene="iutA"
                     /locus_tag="ACHUPB_003194"
     CDS             5774..7969
                     /gene="iutA"
                     /locus_tag="ACHUPB_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0759936.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8520..8663)
                     /locus_tag="ACHUPB_003195"
     CDS             complement(8520..8663)
                     /locus_tag="ACHUPB_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0759937.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2087 a         2232 c         2335 g         2093 t
ORIGIN      
        1 gtgtgggagc tgtttgacta tgaccctgcc ctctgaaaaa ccagccacag atgtggctgc
       61 gcagtgcttc ctgaatgcac tgattcgtga aaccacagac tggaaactga cagaataccc
      121 gccagacgaa ttgctcatcc cgctggatga gcagaaatcg ctccatttca gagtggctta
      181 tttctcccca acccagcatc accgctttgc atttcctgca cgtctggtca cggcatcagg
      241 cagttatcct gtcgacttta ccactctctc ccggcttatt attgataagc tacgccatca
      301 actgtttctg cccgttcccc tctgcgaaac tttccaccag cgcgtgctgg aaagccatgt
      361 ccatacgcaa caggcaattg atgcccgtca tgactgggcc gctctgcgtg aaaaagcgtt
      421 gaattttggc gaggctgagc aggcgctgct gacaggacac gctttccacc ctgcgcctaa
      481 gtctcatgaa ccgtttaacc gacgggaggc tgaacgctac ctgcctgata tggcacccca
      541 cttcccactg cggtggtttt cggtggataa aacgcaaatc gccggtgaaa gtttgcatct
      601 caaccttcaa cagcggctga cgcgatttgc cgcagagaat gcgcctcagt tactcaacga
      661 attaagtgac aaccaatggc tgttcccgtt gcacccgtgg cagggggaat atcttttgca
      721 gcaggggtgg tgccaggcac ttgttgctaa agggctgatt aaagacttag gtgaggccgg
      781 cacgtcgtgg ctgccgacca cctcttcccg ttccctctac tgtgccacca gccgcgatat
      841 gatcaagttc tccctgagcg tacggctgac caactccatc cgtactctgt ccgtgaaaga
      901 agtgaagcgt gggatgcgcc tggcacgcct ggctcaaacc gacggctggc agatgctaca
      961 ggtccgcttc ccgactttcc gggtaatgca ggaggatggc tgggccgggc tgctcgatct
     1021 taacggcaac atcatgcagg aaagtctgtt tgccctgcgt gaaaatctgc tggtggatca
     1081 gccgaaaagc cagactaacg tactggtctc cctgactcag gccgcacctg atggcggtga
     1141 ttcgctgctg gtttcggcgg taaaacgcct gagcgatcgc ctcggtatca ctgtgcaaca
     1201 ggccgcccat gcatgggtcg atgcatactg tcagcaagtg ctaaagccgc tgtttacggc
     1261 tgaagcggat tacggcctgg tgctgctggc gcatcagcaa aatattcttg tccagatgct
     1321 tggggatctg ccggtcggat ttatttaccg tgactgtcag ggcagcgctt ttatgcctca
     1381 cgcgacagac tggctcgatt ctattggcga ggcgcaggcg gaaaatattt tcacccatga
     1441 gcagttgctg cgctatttcc cttattacct gctggttaac tccacttttg ctgtgaccgc
     1501 tgcgctgggg gctgccgggc tggacagcga atcgaatctg atggctcgtg tacgagcatc
     1561 gctggctgaa gtgcgtgatc aggtgactca taaaacctgt ctcaactacg tgctggaaag
     1621 tccgtactgg aacgtaaaag gtaacttttt ctgttatctg aacgatcata acgagaacac
     1681 cattgttgac ccttcggtga tctatttcga tttcgctaac ccgctgcagg ctcaggaggt
     1741 ctgaatgtct gaggcaaaca ttattcacag cagatatgga ctgcgctgtg aaaaactcga
     1801 caagcccctg aatcttggct ggggactgga caatagcgcg gtgttgcact gtcccgggga
     1861 gctgccgaca gggtggctgt gcgatgcgct ggatcagata tttatcgccg caccacaact
     1921 gtcagcagtg gctctcccct gggccgaatg gcgtgaggag ccacaggcgc tgacgctttt
     1981 tgggcaggta aaaagcgaca ttatccatcg tactgccttc tggcagttac cgttatggtt
     2041 gagttcaccg gcaaaccggg cctccggtga aatggttttt gatgcagagc gtgagattta
     2101 tttcccgcag cgcccacccc gtccacaggg tgaggtttac cgccgttacg atccacgtat
     2161 tcgcaggatg ctgagtttcc gcattgccga tcccgtttct gatgcagaac gtttcacccg
     2221 ctggatgaat gatccgcgcg ttgagtattt ctgggagcaa agtggctcac tggaggtaca
     2281 gaccgcctat ctggagcgcc agctcaccgg taaacatgca ttcccgctga ttgggtgctt
     2341 cgacgatcgg ccattcagct atttcgaaat ctactgggcg gcggaagacc gcattggtcg
     2401 tcactattcg tggcagccct ttgaccgtgg cctgcatctg ctggttggtg aacagcaatg
     2461 gcgcggggcc cactatgtgc aaagctggct gcgcgggctg acacattacc tgctgctgga
     2521 tgagccccgt acgcagcgca cagtactgga gccacgcacc gataaccagc gcctgttccg
     2581 ccatcttgag cctgcgggat accggacaat taaagagttc gacttcccac ataagcgctc
     2641 gcgcatggtg atggcggatc gtcatcactt cttcacggag gtcggtctgt aatgaatcac
     2701 aaggactggg attttgttaa ccgtcggctg gtggcaaaaa tgttgtctga gatggagtat
     2761 gagcaggttt tccacgcgga atctcagggc gatgaccact actgcattaa cctgccggga
     2821 gcacaatggc gcttcatcgc tgaacgtggt atctggggct ggctgtggat tgatgcccaa
     2881 acgttgcgct gcacggacga accagtactg gcgcagacgc tgctgatgca gctaaagccg
     2941 gtgctgtcaa tgagtgatgc cactgttgca gagcatatgc aggatttgta tgccacgcta
     3001 ctgggcgacc tgcaactgct gaaggcccgt cgcggactga gtgccagtga cctgattaat
     3061 cttgatgccg atcgtctgca atgcctgctg agcggtcatc ctaaattcgt ttttaataaa
     3121 ggtcgccgtg gctggggcaa agaggcgctg gaacgatatg cgccagagta taccaacacc
     3181 ttcagactgc actggctggc ggtaaaacgt gaacatatga tctggcgctg cgataacgat
     3241 ctggatattc agcagttgtt gactgccgca atggatccgc aggagtttac acgcttcagt
     3301 caggtctggc aggaaaacgg gctggatcac aactggctac cgctgccggt acatccgtgg
     3361 cagtggcagc aaaaaatcgc cacagacttc atcgctgatt ttgtcgaagg caggatggtg
     3421 tctctcggcg agtttggcga ccagtggctc gcccagcagt cgctgcgtac cctgaccaac
     3481 gccagccgcc ggggagggct ggatatcaag ctgccgctga ccatctacaa cacctcatgc
     3541 taccggggaa tccctggcag atatatcgct gccggaccac tggcttcacg ctggctgcaa
     3601 caggtttttg cgaccgacgc caccctggtg caaagcggcg cagtgatcct tggtgaaccg
     3661 gctgcaggct atgtgtccca tgaaggctat gccgcgcttg cccgggctcc ctatcgctac
     3721 caggaaatgc ttggtgttat ctggcgggag aatccgtgcc gctggctgaa accggatgaa
     3781 agtcctgttc tgatggcaac actgatggag tgcgacgaaa acaatcagcc gctggcaggc
     3841 gcatatatcg accgctccgg actggacgct gaaacctggc ttacgcaatt gttccgggtg
     3901 gttgtggttc cgctgtatca cctgctttgc cgctacggtg tcgcgcttat tgcacacgga
     3961 caaaatataa cgctcgccat gaaagagggg gtaccacagc gtgttctgct gaaagacttc
     4021 cagggcgata tgcggctggt gaaagaagcg tttcccgaaa tggactcttt gcctcaggag
     4081 gtgcgtgatg ccacatcccg tctgagtgcg gactacttaa tccatgattt gcagacgggt
     4141 cacttcgtga cagtactgcg ttttatttcg ccactgatgg ttcgtcttgg cgtacctgaa
     4201 aggcgatttt atcaactgct ggcagcagtg ttgagtgatt acatgaacaa acatccacaa
     4261 atggcagagc gttttgcgct tttctcactc ttcaggccac aaatcattcg cgtggtgctg
     4321 aacccggtaa aactgacctg gccggacctg gatggcggca gtcgcatgct gccgaattac
     4381 cttgagaatc tgcaaaatcc gctgtggctg gtaactcagg aatatgaatc atgaaaaaaa
     4441 gtgtcgattt tattggtgtc gggacagggc catttaatct cagcattgct gcattgtcac
     4501 atcagatcga agaactgaac tgtctcttct ttgacgaaca tcctcatttt tcctggcatc
     4561 cgggtatgct ggtaccggat tgtcatatgc agaccgtctt tctgaaagat ctggtcagtg
     4621 cagttgcacc tacaaatccc tacagctttg ttaactatct ggtgaagcac aaaaagttct
     4681 atcgcttcct tacaagcagg ctacgtacag tatcccgtga agagttttct gactatctcc
     4741 gctgggctgc tgaagatatg aataacctgt atttcagtca taccgttgaa aatattgatt
     4801 tcgacaaaaa aagtcgattg tttctggttc agaccagtcg gggagaatat tttgcccgca
     4861 atatctgcct tggtacagga aaacaacctt atttaccacc ctgtgtgaag catgtgacac
     4921 aatcctgttt ccatgccagt gaaatgaatc ttcgtcggcc ggacctgagt ggaaaacgga
     4981 taaccgtggt tggtggagga cagagtggtg cagacctgtt ccttaatgca ttacgcgggg
     5041 aatggggaga agcggcggaa ataaactggg tctcacggcg taataatttt aacgcactgg
     5101 atgaggctgc ttttgctgat gagtatttta cacctgaata tatttcaggc ttctccggac
     5161 tgaaggaaga tattcgccat cagttactgg atgagcagaa aatgacatcg gatggcatca
     5221 ctgccgattc tttactgacc atttatcgtg agttgtacca ccgttttgaa gttctgagaa
     5281 aaccaagaaa tatccgtctg ctacccagtc gctcggtaac aaccctggaa agtagtggtc
     5341 cgggctggag gttattgatg gaacatcatc tggatcaggg cagagagagc ctggaaagtg
     5401 atgtggtgat tttcgccaca ggttaccgtt ctgcattgcc acaaatactt ccctcactga
     5461 tgcccttgat caccatgcac gataagaaca cctttaaagt gcgtgatgac ttcactctgg
     5521 aatggagtgg cccgaaagaa aataacatct ttgcggtcaa cgccagcatg caaactcatg
     5581 gcatcgccga accccagctc agcctgatgg cctggcgatc tgcacgtatt cttaatcgcg
     5641 tactggggcg tgatttattc gatctcagta tgccgcccgc actgattcag tggcgcagcg
     5701 gcagccggaa aaaaccgcag ccggaggctg cttctttaac tcactataca gcaaatattc
     5761 aggaataacg atgatgcgca aaaagtatat gccccgggct cttggtccgc tgcttcttgt
     5821 cgtgctgtca ccagctgtcg cccagcaaaa cgatgataat gagatcatag tgtctgccag
     5881 ccgcagcaat cgaactgtag cggagatggc gcaaaccacc tgggttatcg aaaatgccga
     5941 actggagcag cagattcagg gcggtaaaga gctgaaagac gcactggctc agttaatccc
     6001 cggccttgat gtcagcagcc agagtcgaac caactacggt atgaacatgc gtggccgccc
     6061 gctggttgtc ctgattgacg gtgtgcgcct caactcttca cgttccgaca gccgacaact
     6121 ggactctgtc gatcctttta atatcgacca tattgaagtg atctccggcg cgacggccct
     6181 gtacggtggc gggagtaccg gagggttgat caacatcgtg accaaaaaag gtcagccgga
     6241 aaccatgatg gagtttgagg ctggcacaaa aagtggcttt aacagcagta aagatcacga
     6301 tgagcgcatt gccggtgctg tctccggcgg aaatgaccat atctccggac gtctttccgt
     6361 ggcatatcag aaatttggcg gctggtttga cggtaacggc gatgccaccc tgcttgataa
     6421 cacccagacc ggcctgcagc actccaatcg gctggacatc atgggaaccg gtacgctgaa
     6481 catcgatgaa tcccggcagc ttcaactgat aacgcagtac tataaaagtc agggagacga
     6541 caattacggg cttaatctcg ggaaaggctt ttccgccatc agcgggagca gcacaccata
     6601 cgtcagtaag gggctgaatt ctgaccgcat tcccggcact gagcggcatt tgatcagcct
     6661 gcagtactct gacagtgatt tcctgggaca ggaactggtc ggtcaggttt actaccgcga
     6721 tgagtcgttg cggtactacc cgttcccgac ggtaaatgcg aataaacagg cgacagcttt
     6781 ctcctcgtca cagcaggata ccgaccagta cggcatgaaa ctgactctga acagccaact
     6841 gatggacggc tggcaaatca cctgggggct ggatgctgag catgagcgct ttacctccaa
     6901 ccagatgttc ttcgatctgg ctcaggcaag tgcttccgga gggctgaaca accataagat
     6961 ttacaccacc gggcgctatc cgtcatatga catcaccaat ctggcggcct tcctgcaatc
     7021 cagctatgac attaatgata tttttaccgt tagcggtggc gtacgctatc agtatactga
     7081 gaacagggta gatgatttca tcgactacac gcagcaacag aagattgctg ccgggaaggc
     7141 gatatctgcc gacgccattc ctggtggttc ggtagattac gataactttc tgttcaatgc
     7201 tggtctgctg atgcacatca ccgaacgtca gcaggcatgg ttcaattttt cccagggggt
     7261 ggcattgccg gatccgggga aatattatgg tcgcggcatc tatggtgcag cagtgaacgg
     7321 ccatctttcc ctgacaaaga gcgtgaacgt cagcgacagt aagctggaag gcgtgaaagt
     7381 cgattcttat gaactgggct ggcgctttac cggtgacaac ctgcggactc aaatcgcggc
     7441 atattactcg ctttccaata agagcgtgga aaggaataaa gatctgacca tcagtgtgaa
     7501 ggacgacagg cgccgtattt acggcgtgga aggtgcggtg gacttcctga tcccggatac
     7561 tgactggagt accggtgtga acttcaatgt gctgaaaacc gagtcgaaag tgaacggtca
     7621 atggcaaaaa tatgacgtga aggaatcaag tccatcgaaa gcgacagctt acattaactg
     7681 ggcgccggaa ccgtggagtc tgcgtgtaca gagcaccact tctttcgacg taagcgatgc
     7741 agagggtaac gatattaatg gttacactac cgtcgatttt atcagtagtt ggcagcttcc
     7801 ggtgggaaca ctcagcttca gcgttgagaa cctcttcgac cgtgactata ccactgtctg
     7861 gggacagcgt gcacctctgt actactgccc gggttacggc cctgcttcac tgtacgacta
     7921 caaaggccgg ggccgaacct ttggtctgaa ctactcagtg ctgttctgac ggggatgctt
     7981 ttgcaacaag ggtatgctgt aaacaacatg gtccttggtg gccatgttgt ttacagccag
     8041 tataatatac agccttaaca agatattccg tctccggaat ggactgttca ccactgaata
     8101 acattatttc tgttttatgg atctttgact gaaatttatc gcatatcctc atttcattgt
     8161 ccgtctgaaa actaacctta caagtaagcg tcgtctcagc accgtctggc agatcctgaa
     8221 attcctgaga ggatgatctg cgcccaatat cctgagccgg aactttgctg caatgaaggc
     8281 caacgaaaaa tgggggagta tgttatagag tacttgctac agggtaaaaa gaggtacctg
     8341 tcgctggttc tccgttcagt ttaaccggga gattttctcc tgcgcctgtc ataaagtccg
     8401 gtaatggaga ttgttaatac gatggtgcac gatgcattgt cggtgcccgg cccggaggat
     8461 gccccgtaac tacatacgga tcagggttgg caatatcgaa tggcaggcta taggcaaagt
     8521 taaagcccca tggagtagca caaaatatgc cacacaaagg aaacggtctg aataacgcag
     8581 tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc taattcgccg
     8641 tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa tattaaacat
     8701 tacccctccc cgcattgggc aaaaaaaaaa caaaaaacga tcaatta
//
LOCUS       DAZOZD010000168         8670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZOZD010000168 DAZOZD010000000
VERSION     DAZOZD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.168"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(278..541)
                     /gene="rpsT"
                     /locus_tag="ACHUPB_003196"
     CDS             complement(278..541)
                     /gene="rpsT"
                     /locus_tag="ACHUPB_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0759938.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            643..861
                     /locus_tag="ACHUPB_003197"
     CDS             643..861
                     /locus_tag="ACHUPB_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0759939.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            869..1810
                     /gene="ribF"
                     /locus_tag="ACHUPB_003198"
     CDS             869..1810
                     /gene="ribF"
                     /locus_tag="ACHUPB_003198"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0759940.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1853..4669
                     /gene="ileS"
                     /locus_tag="ACHUPB_003199"
     CDS             1853..4669
                     /gene="ileS"
                     /locus_tag="ACHUPB_003199"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0759941.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4669..5163
                     /gene="lspA"
                     /locus_tag="ACHUPB_003200"
     CDS             4669..5163
                     /gene="lspA"
                     /locus_tag="ACHUPB_003200"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0759942.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5288..5737
                     /gene="fkpB"
                     /locus_tag="ACHUPB_003201"
     CDS             5288..5737
                     /gene="fkpB"
                     /locus_tag="ACHUPB_003201"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0759943.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5739..6689
                     /gene="ispH"
                     /locus_tag="ACHUPB_003202"
                     /gene_synonym="lytB"
     CDS             5739..6689
                     /gene="ispH"
                     /locus_tag="ACHUPB_003202"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0759944.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6755..7669
                     /gene="rihC"
                     /locus_tag="ACHUPB_003203"
     CDS             6755..7669
                     /gene="rihC"
                     /locus_tag="ACHUPB_003203"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0759945.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7836..8657
                     /gene="dapB"
                     /locus_tag="ACHUPB_003204"
     CDS             7836..8657
                     /gene="dapB"
                     /locus_tag="ACHUPB_003204"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0759946.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1980 a         2165 c         2458 g         2067 t
ORIGIN      
        1 cttaagttta atacctcaat gtgtatcaca atatccacat tctttgtagg ggagtctgga
       61 gattgagtag atattcttgt tcagaatgta tcagccgatg gttctacgat tcttaagcca
      121 cgaagagttc agaaagtgta acggcatgtc tgcaaaagcc agcatctttt gactatctgg
      181 caaccggtag tgtgttctct cacgcatcat aaaagcagca ggcataaaaa aaacccgctt
      241 gcgcgggctt tttcacaaag cttcagcaaa ttggcgatta agccagtttg ttgatctgtg
      301 cagtcaggtt agccttatga cgtgcagctt tgtttttgtg gatcagacct ttagcagcct
      361 gacggtccac gatcggttgc atttcgttaa atgctttctg tgcagcagct ttgtcgccag
      421 cttcgatagc tgcgtatact ttcttgatga aagtacgcat catagagcga cggcttgcgt
      481 tgtgcttacg agccttttca gactgaatgg cgcgcttctt agctgatttg atattagcca
      541 aggtccaact cccaaatgtg ttctatatgg acaattcaaa ggccgaggaa tatgcccttt
      601 tagccttctt ttgtcaatgg atttgtcaaa taagcgccgt taatgtgccg gcactcgtta
      661 cgtagtgatg gcgcaggatt ctaccagctt gcggggtgtg aatacagctt ttccgcgata
      721 aaaattgcag caggcggtca ctttcttccc gtgatttgcg ccatggcaat gaaaagccac
      781 ttctttctga tttcggtact caatcgccgg ttaaccttga ccgctgtaca aggtatactc
      841 ggacgatttt cactgttttg agccagacat gaagctgata cgcggcatac ataatctcag
      901 ccaggccccg caagaagggt gtgtgctgac tattggtaat ttcgacggcg tgcatcgcgg
      961 tcatcgcgcg ctgttacagg gcttgcagaa agaagggcgc aagcgcaact taccggtgat
     1021 ggtgatgctt tttgaacctc aaccactgga actgtttgct accgataaag ccccggcaag
     1081 actgacccgg ctgcgggaaa aactgcgtta ccttgcagag tgtggcgttg attacgtgct
     1141 gtgcgtgcgt ttcgacaggc gtttcgcggc gttaaccgcg caaaatttca tcagcgatct
     1201 tctggtgaag catttgcgcg taaaatttct tgccgtaggg gatgatttcc gctttggcgc
     1261 tggtcgtgaa ggcgatttct tgttattaca gaaagctagc atggaatacg gcttcgatat
     1321 cactagtacg caaacttttt gcgaaggtgg cgtgcgtatt agcagcaccg ctgtgcgtca
     1381 ggcgctggcg gatgacaatc tggctctggc agaaagttta ctggggcacc cgtttgccat
     1441 ctccgggcgt gtagtccacg gtgatgaatt agggcgcact ataggtttcc cgacggcgaa
     1501 tgtaccgcta cgccgtcagg tttccccggt gaaaggggtt tatgcggtag aagtgctggg
     1561 cctcggtgaa aagccgttac ccggcgtggc aaacatcgga acacgcccaa cggttgccgg
     1621 tattcgccag cagctggaag tgcatttgtt agatgttgca atggaccttt acggtcgcca
     1681 tatacaagta gtgctgcgta aaaaaatacg caatgagcag cgatttgcgt cgctggacga
     1741 actgaaagcg cagattgcgc gtgatgaatt aaccgcccgc gaattttttg ggctaacaaa
     1801 accggcttaa gcctgttatg taatcaaacc gaaacacgga accgagaatc tgatgagtga
     1861 ctataaatca accctgaatt tgccggaaac agggttcccg atgcgtggcg atctcgccaa
     1921 gcgcgaaccg ggaatgctgg cgcgttggac tgatgatgat ctgtacggca tcatccgtgc
     1981 ggctaaaaaa ggcaaaaaaa ccttcattct gcatgatggc cctccttatg cgaatggcag
     2041 cattcatatt ggtcactcgg ttaacaagat tctgaaagac attatcgtga agtccaaagg
     2101 gctttccggt tatgactcgc cgtatgtgcc tggctgggac tgccacggtc tgccgatcga
     2161 gctgaaagta gagcaagaat acggtaagcc gggtgagaaa ttcaccgccg ccgagttccg
     2221 cgccaagtgc cgcgaatacg cggcgaccca ggttgacggt caacgcaaag actttatccg
     2281 tctgggcgtg ctgggcgact ggtcgcaccc gtacctgacc atggacttca aaactgaagc
     2341 caacatcatc cgcgcgctgg gcaaaatcat cggcaacggt cacctgcaca aaggcgcgaa
     2401 gccagttcac tggtgcgttg actgccgttc tgcgctggcg gaagcggaag ttgagtatta
     2461 cgacaaaact tctccgtcca tcgacgttgc ttttcaggcg gtcgatcagg atgcactgaa
     2521 agcaaaattt gccgtaagca acgttaacgg cccaatctcg ctggtgatct ggaccactac
     2581 gccgtggact ctgcctgcga accgcgcaat ctctattgca cctgatttcg actatgcgct
     2641 ggtgcagatc gacggtcagg ccgtgattct ggcgaaagat ctggttgaaa gcgtaatgca
     2701 gcgtatcggc gtgaccgatt acaccattct cggcacggta aaaggtgcgg agcttgagtt
     2761 gctgcgcttt acccatccgt ttatgggctt cgacgttccg gcaatcctcg gcgatcacgt
     2821 taccctagat gccggtaccg gtgccgttca caccgcgcct ggccacggcc cggacgacta
     2881 tgtgatcggt cagaaatacg gcctggaaac cgctaacccg gttggcccgg acggcactta
     2941 tctgccgggc acttatccga cgctggatgg cgtgaacgtc ttcaaagcga acgacatcgt
     3001 cgttgcgctg ctgcaggaaa aaggcgcgct gctgcacgtt gagaaaatgc agcacagcta
     3061 tccgtgctgc tggcgtcaca aaacgccgat catcttccgc gcgacgccgc agtggttcgt
     3121 cagcatggat cagaaaggtc tgcgtgcgca gtcactgaaa gagatcaaag gcgtgcagtg
     3181 gatcccggac tggggccagg cgcgtatcga gtcgatggtt gctaaccgtc ctgactggtg
     3241 tatctcccgt cagcgcacct ggggcgtacc gatgtcactg ttcgtgcaca aagacacgga
     3301 agagctgcat ccgcgtactc tcgaactaat ggaagaagtg gcaaaacgcg ttgaagttga
     3361 cggcatccag gcgtggtggg atcttgatgc gaaagagatc ctcggcgatg aagctgatca
     3421 gtacgtgaaa gtgccggaca cattggatgt atggtttgac tccggatcta cccactcttc
     3481 tgttgttgac gtgcgtccgg aatttgccgg tcacgcagcg gacatgtatc tggaaggttc
     3541 tgaccagcac cgtggttggt tcatgtcttc cctaatgatc tccaccgcga tgaaaggcaa
     3601 agcgccgtat cgtcaggtac tgacccacgg ctttaccgtg gatggtcagg gtcgcaagat
     3661 gtctaaatcc atcggcaata ccgtttcgcc gcaggatgtg atgaacaaac tgggcgcgga
     3721 tattctgcgt ctgtgggtgg catcaaccga ctacaccggt gaaatggccg tttctgacga
     3781 gatcctgaaa cgtgctgccg atagctatcg tcgtatccgt aacaccgcgc gcttcctgct
     3841 ggcaaacctg aacggttttg atccagcaaa agatatggtg aaaccggaag agatggtggt
     3901 actggatcgc tgggccgtag gttgtgcgaa agcggcacag gaagacatcc tcaaggcgta
     3961 cgaagcatac gatttccacg aagtggtaca gcgtctgatg cgcttctgct ccgttgagat
     4021 gggttccttc tacctcgaca tcatcaaaga ccgtcagtac accgccaaag cggacagtgt
     4081 ggcgcgtcgt agctgccaga ctgcgctgta tcacatcgca gaagcgctgg tgcgctggat
     4141 ggcaccaatc ctctccttca ccgctgatga agtgtggggc tacctgccgg gcgaacgtga
     4201 aaaatacgtc ttcaccggtg agtggtacga aggcctgttt ggcctggcag acagtgaagc
     4261 gatgaacgat gcgttctggg acgagctgtt gaaagtgcgt ggcgaagtga acaaagtcat
     4321 tgagcaagcg cgtgccgaca agaaagtggg tggctcgctg gaagcggcag taaccttgta
     4381 tgcagaaccg gaactgtcgg cgaaactgac cgcgctgggc gatgaattac gatttgtcct
     4441 gttgacctcc ggcgctaccg ttgcagacta taacgacgca cctgctgatg ctcagcagag
     4501 cgaagtactc aaagggctga aagtcgcgtt gagtaaagcc gaaggtgaga agtgcccacg
     4561 ctgctggcac tacacccagg atgtcggcaa ggtggcggaa cacgcagaaa tctgcggccg
     4621 ctgtgtcagc aacgtcgccg gtgacggtga aaaacgtaag tttgcctgat gagtcaatcg
     4681 atctgttcaa cagggctacg ctggctgtgg ctggtggtag tcgtgctgat tatcgatctg
     4741 ggcagcaaat acctgatcct ccagaacttt gctctggggg atacggtccc gctgttcccg
     4801 tcgcttaatc tgcattatgc gcgtaactat ggcgcggcgt ttagtttcct tgccgatagc
     4861 ggcggctggc agcgttggtt ctttgccggt attgcgattg gtattagcgt gatcctggca
     4921 gtgatgatgt atcgctcgaa ggccacgcag aagctaaaca atatcgctta cgcgctgatt
     4981 attggcggcg cgctgggcaa cctgttcgac cgcctgtggc acggcttcgt tgtcgatatg
     5041 atcgacttct acgtcggcga ctggcacttc gccaccttca accttgccga tactgccatc
     5101 tgtgtcggtg cggcactgat tgtgctggaa ggttttttgc cttctagagc gaaaaaacaa
     5161 taataaaccc tgccggatgc gatgctgacg catcttatcc ggcctacaga ttgctgcgaa
     5221 atcgtaggcc ggataaggcg tttacgccgc atccggcaaa aatcctcaaa tataagagca
     5281 aacctgcatg tctgaatctg tacagagcaa tagcgccgtc ctggtgcact tcacgctaaa
     5341 actcgacgat ggcaccaccg ccgagtctac ccgcaacaac ggtaaaccgg cgctgttccg
     5401 cctgggtgat gcttctcttt ctgaagggct ggagcaacac ctgttggggc tgaaagtggg
     5461 cgataaaacc accttctcgt tggagccaga tgcggcgttt ggcgtgccgt caccggacct
     5521 gattcagtac ttttcccgcc gtgaatttat ggatgcaggc gagccagaaa ttggcgcaat
     5581 catgcttttt accgcaatgg atggcagtga gatgcctggc gtgatccgcg aaattaacgg
     5641 cgactccatt accgttgatt tcaaccatcc gctggccggg cagaccgttc attttgatat
     5701 tgaagtgctg gaaatcgatc cggcattgga ggcgtaacat gcagatcctg ttggccaacc
     5761 cgcgtggttt ttgtgccggg gtagaccgcg ctatcagcat tgttgaaaac gcgctggcca
     5821 tttacggcgc accgatatat gtccgtcacg aagtggtaca taaccgctat gtggtcgata
     5881 gcttgcgtga gcgtggggct atctttattg agcagattag cgaagtaccg gacggcgcga
     5941 tcctgatttt ctccgcacac ggtgtttctc aggcggtacg taacgaagca aaaagccgcg
     6001 atttgacggt gtttgatgcc acctgtccgc tggtgaccaa agtgcatatg gaagtcgccc
     6061 gcgccagccg tcgtggcgaa gagtcgattc tcatcggcca cgccgggcat ccggaagtgg
     6121 aaggtacaat gggccagtac agtaacccgg aagggggaat gtatctggtc gaatcgccgg
     6181 acgatgtgtg gaaactgacg gtcaaaaacg aagagaagct ctcctttatg acccagacca
     6241 cgctgtcggt ggatgacacg tctgatgtga tcgacgcgct gcgtaaacgc ttcccgaaaa
     6301 ttgtcggtcc gcgcaaagat gacatctgct acgccacgac taaccgtcag gaagcggtac
     6361 gcgccctggc agaacaggcg gaagttgtgt tggtggtcgg ttcgaaaaac tcctccaact
     6421 ccaaccgtct ggcggagctg gcccagcgta tgggcaaacg cgcgtttttg attgacgatg
     6481 cgaaagatat ccaggaagag tgggtgaaag aggttaaatg cgtcggcgtg actgcgggcg
     6541 catcggctcc ggatattctg gtgcaaaatg tggtggcacg tttgcagcag ctgggcggtg
     6601 gtgaagccat tccgctggaa ggccgtgaag aaaacattgt tttcgaagtg ccgaaagagc
     6661 tgcgtgtcga tattcgtgaa gtcgattaag tcattagcag cctaagttat gcgaaaatgc
     6721 cggtcttgtt accggcattt tttatggaga aaacatgcgt ttacctatct tcctcgatac
     6781 tgaccccggc attgacgatg ccgtcgccat tgccgccgcg atttttgccc ccgaactcga
     6841 cctgcaactg atgaccaccg tcgcgggtaa tgtctcggtt gagaaaacca cccgcaacgc
     6901 cctgcaactg ctgcatttct ggaatgcgga gattccgctc gcccagggag ccgctgtgcc
     6961 gctggtacgc gcaccgcgtg atgcagcatc tgtgcacggc gaatcgggaa tggctggcta
     7021 cgactttgtt gagcacaacc gaaagccaat cgggatacca gcgtttctgg cgattcgtga
     7081 tgccctgatg cgtgcaccag agcctgttac cctggtggcc attggcccgt taaccaatat
     7141 tgcgctgtta ctttcacaat gcccggaatg taaaccgtat attcgtcgtc tggtgatcat
     7201 gggcggttct tctggtcgcg gcaactgtac accaaatgcc gagtttaata tcgctgccga
     7261 tccagaagcg gctgcctgtg tcttccgtag cggaatcgaa atcgtcatgt gcggtctgga
     7321 tgtcaccaat caggcaatat taacccctga ctatctcgct actttgccgg agttgaatcg
     7381 caccggaaaa atgcttcatg cactgtttag ccactaccgt agcggcagta tgcaaagcgg
     7441 tctgcgaatg cacgatctct gcgccatcgc ctggctggtg cgcccggact tattcaccct
     7501 caagccgtgt tttgtcgcgg tagaaactca aggcgaattt acctcaggca cgacggtggt
     7561 tgatatcgac ggttgcctgg gcaaaccagc caatgtaaag gtggcattgg atctggatgt
     7621 gaaaggcttc cagcagtggg tggctgaggt gctggctctg gcgtcgtaac ctgtcacatg
     7681 ttatcggcat gcagtcattc atcgactcat gcctttcact gatatccctc cctgtttatc
     7741 attaatttct aattatcagc gtttttggct ggcggcgtag cgatgcgctg gttactctga
     7801 aaacggtcta tgcaaattaa caaaagagaa tagctatgca tgatgcaaac atccgcgttg
     7861 ccatcgcggg agccgggggg cgtatgggcc gccagttgat tcaggcggcg ctggcattag
     7921 aaggcgtgca gctgggcgcg gcgctggagc gcgaaggatc ttctttactg ggcagcgacg
     7981 ccggtgagct ggctggtgca gggaaaacag gcgttaccgt gcaaagcagc ctcgatgcgg
     8041 taaaagatga ttttgatgtg tttatcgatt ttacccgtcc ggaaggtacg ctgaaccatc
     8101 tcgctttttg tcgccagcat ggcaaaggga tggtgatcgg cactacgggg tttgacgaag
     8161 ccggtaaaca ggcgattcgt gacgccgccg ccgatattgc gattgtcttt gccgccaatt
     8221 ttagcgttgg cgttaacgtc atgcttaagc tgctggagaa agcagccaaa gtgatgggtg
     8281 actacaccga tatcgaaatt attgaagcac atcatagaca taaagttgat gcgccgtcag
     8341 gcaccgcact ggcaatggga gaggcgatcg cccacgccct tgataaagat ctgaaagatt
     8401 gcgcggtcta cagtcgtgaa ggccacaccg gtgaacgtgt gcctggcacc attggttttg
     8461 ccaccgtgcg tgcaggtgac atcgttggtg aacataccgc gatgtttgcc gatattggcg
     8521 agcgtctgga gatcacccat aaggcgtcca gccgtatgac atttgctaac ggcgcggtaa
     8581 gatcggcttt gtggttgagt ggtaaggaaa atggtctttt tgatatgcga gatgtactta
     8641 atctcaataa tttgtaacca caaaatattt
//
LOCUS       DAZOZD010000169         8634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOZD010000169 DAZOZD010000000
VERSION     DAZOZD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.169"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUPB_003205"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUPB_003205"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0759947.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUPB_003206"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUPB_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0759948.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHUPB_003207"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHUPB_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0759949.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHUPB_003208"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHUPB_003208"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0759950.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHUPB_003209"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHUPB_003209"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0759951.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHUPB_003210"
     CDS             complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHUPB_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0759952.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHUPB_003211"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHUPB_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0759953.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2179 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaa
//
LOCUS       DAZOZD010000170         8573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZOZD010000170 DAZOZD010000000
VERSION     DAZOZD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.170"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(152..1816)
                     /gene="glnS"
                     /locus_tag="ACHUPB_003212"
     CDS             complement(152..1816)
                     /gene="glnS"
                     /locus_tag="ACHUPB_003212"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0759954.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2076..4022)
                     /gene="nagE"
                     /locus_tag="ACHUPB_003213"
     CDS             complement(2076..4022)
                     /gene="nagE"
                     /locus_tag="ACHUPB_003213"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0759955.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4355..5155
                     /gene="nagB"
                     /locus_tag="ACHUPB_003214"
     CDS             4355..5155
                     /gene="nagB"
                     /locus_tag="ACHUPB_003214"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0759956.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5215..6363
                     /gene="nagA"
                     /locus_tag="ACHUPB_003215"
     CDS             5215..6363
                     /gene="nagA"
                     /locus_tag="ACHUPB_003215"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0759957.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6372..7592
                     /gene="nagC"
                     /locus_tag="ACHUPB_003216"
                     /gene_synonym="nagR"
     CDS             6372..7592
                     /gene="nagC"
                     /locus_tag="ACHUPB_003216"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0759958.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7640..8392
                     /gene="nagD"
                     /locus_tag="ACHUPB_003217"
     CDS             7640..8392
                     /gene="nagD"
                     /locus_tag="ACHUPB_003217"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR0759959.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8443..8573)
                     /locus_tag="ACHUPB_003218"
                     /pseudo
     CDS             complement(8443..8573)
                     /locus_tag="ACHUPB_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2098 a         2261 c         2183 g         2031 t
ORIGIN      
        1 cggcctacaa aagcacgcaa actcaatata ttgcagagat catgtaggcc tgataagcgt
       61 agtgcatcag gcaatttagc gtttgaaact gtttgtctgt tccttgccgg atgcgacgta
      121 aacgccccat ccggcatagc gaaacttaaa attactcgcc tactttcgcc caggtatcac
      181 gcagcccaac ggtgcggtta aataccggtt tttccgccgt agaatggcgg ctatcgaggc
      241 agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct ttcagcgacg
      301 gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac aggaaatcat
      361 ccgcagcacc agggttcggc acgctgaaca gacggtcgta cagacggatt tcaaccggca
      421 gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca tctgccggat
      481 ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatattg ccttcggcat
      541 ctttctccac gcgttctgcc ttaatgacgt aagcattacg cagacgcact tctttaccca
      601 gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa
      661 tctcaccgct aaacggcacc tggcggctac ccatttccgg tttgttcgga tggttcggca
      721 tggtaaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc ggatcgataa
      781 ccgccattgc gcgcggggcg ttttcgttga gatcttcacg gatgcaggat tccagcgacg
      841 ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac tcacgaatag
      901 aagcagcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc gggtcatccc
      961 agccttcaac gtgcttgtcg gtcaccagca ggttcagctt acgcttggac atcacagtgt
     1021 attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt
     1081 ccagcaccca gtcgtacagg cgacggttgt cctggaactc aagcgtacac agagagtgcg
     1141 taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc
     1201 acttgttgcc agtctggtgg tgttcagcaa atttaatgcg gtacagcacc ggatcgcgca
     1261 tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa
     1321 aaccaccggc acgcattttt tcgaacagcg ccaggttctc ttcaacgctg cggtcgcggt
     1381 acgggctgtt tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg
     1441 tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag gcgtggagct
     1501 gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct aaccactcta
     1561 cgtcgttttt gatcgactca acatactcga tatcttcttt taccgggtta gtgtcgtcga
     1621 aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg cagatagatt
     1681 tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg tgtactgtgg
     1741 tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta gtcgggcggg
     1801 cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg gcgtatgatc
     1861 ttataagcgg gacaggctga caactgttag ttagcgaaaa tcgaaaatct cactgagatt
     1921 atggggatga ttgatgaaaa aaaaaagcgg cagagaagat ctccaccgct tttgccagtc
     1981 ccgcgtcatt gttggatgcg cgtcttatcc aaccaggact gagcttttgt aggcctgata
     2041 agacgcgtca agcgtcgcat caggcataaa gcagattact ttttgatttc atacagcggt
     2101 gtttgacccg ccacaacatt gccctgagct ttaatgatca agccactgaa atcgtcgata
     2161 ttgctgcaaa ccaccgggct aatcatcgaa cgggcgttag cgttcaggta atccagatcc
     2221 atttccagaa tcggttgccc tgcgctcacc tgcgcgccct cttccaccag acgtttaaag
     2281 cctttacctt ccagcgctac ggtgtcgata cccatgtgga cgacaatctc cgcgcctttt
     2341 tcggtttcta ggcagaacgc gtggttggtg ttgaagattt tcacgattgt ccctgcggct
     2401 ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac cgctttgctg
     2461 gcgaatgctt cgtcagaaac ctgatccagt gccacgacat caccggtaat cggcgatacc
     2521 agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg agcggcagtt
     2581 gccggagttg cttcagctga cgcagctgct accgggccac gggcaacgac tttcttcatc
     2641 gcatcgccaa tggattctgc tttcgcgcca acaatcacct gaatggtctg tttgttcagt
     2701 tttactacac cagaagcacc cagacgttta cacatcgcat cgttgacgcg agctgagtca
     2761 gccacggtaa ggcgcagacg ggtaatacag gcgtcaatcg ctttcaggtt gtcagtaccg
     2821 ccaaccgcag caatatagtt ggttgccagt tgagtcagac cttcttcagt attgctgtta
     2881 gcctcttcag ttacgatctc gtcttcttta tcttcacgac ccggcgtttt caggttgaac
     2941 atgcggataa ccaaactgaa caccacgaag tagatagcga agaagacaac acccatcacc
     3001 agcagcatcc agacgttctg gctggcggcc ggcaggttat acatcaacgc gtagtcgata
     3061 gcccccgcag agaaagagaa gccagcatgg atacccagca gcgttgccac aaacaggctg
     3121 ataccggtca gcagtgcgtg caggaggtac agcagcggag caaggaacat gaacaggaat
     3181 tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag cagcatcccg
     3241 ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc
     3301 agaccgaaca tcatgatcgg gaagaagccg gacatgaaca tccccgcggt gccgtcacca
     3361 gcgtagaaac ggttgatgtc gccgtggaaa accgtacccg ccgcgttggt gaattcacca
     3421 atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat cagcagacgg
     3481 ttgatgaaac caaagatacc ggaacccagc gcgcctgcag aaacgatcca ctcgccgcct
     3541 gcatggatag cgtgctgtac tggcggccag acgtaaccaa aaatggccgc cagcaccagg
     3601 cagaaaaagc cggtggcaat cggcacaaag cgtttgccgc cgaagaagct caggaagtcc
     3661 ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc ggtaatgata
     3721 cccgccagta cacccatgtt aatttctggg ttgatggtca ccatcgcttt ggttaacaca
     3781 aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga ccagctggat
     3841 gccacaccga tggcgaagat taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca
     3901 ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagtgc cgccaccggc
     3961 agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc taaaatattc
     4021 atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt ttattttgct
     4081 gcaaattgta ctgccgatgt tctgtaatca gattgttaga tcatctgcta cagagtgtgt
     4141 gaaaatttaa ttcgtatcgc aaattaaacg cgtgcctttt gtgagttttg tcaccaaata
     4201 tcgttatcat cactcccttt tactggctaa agcagaaaac ttattttatc attcaaaaaa
     4261 tcaggtcgga ttgacgcctg tctgcgcaaa tccaggttac gcttaaagat gcctaatccg
     4321 ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc taactaccgc
     4381 tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc
     4441 gactgccgat cgtccgtttg ttctgggcct gccgactggc ggcacgccga tgaccaccta
     4501 taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt
     4561 caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact acagctttat
     4621 gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aacatcaacc ttctcaacgg
     4681 caacgccccg gatatcgacg ccgaatgccg ccagtatgaa gaaaaaatcc gttcttacgg
     4741 aaaaattcat ctgtttatgg gcggtgtagg taacgacggt catattgcat ttaacgaacc
     4801 ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc
     4861 gaactctcgt ttctttgata acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg
     4921 tgttggtaca ttgctggatg ccgaagaagt gatgattctg gtgctgggta gccagaaagc
     4981 actggcactg caggctgcag ttgaaggttg cgtgaaccac atgtggacca tcagctgtct
     5041 gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct tccaccatgg agctgaaagt
     5101 taagacttta agatatttca atgaattaga agcagaaaat atcaaaggtc tgtaattgtt
     5161 atccctgccc tggctccttg ctcagggcaa tattttttaa aatcgggggg cagaatgtat
     5221 gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca cgcggttgtt
     5281 atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa
     5341 caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca gttaaacggc
     5401 tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac gctggaaatc
     5461 atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct tatcaccacc
     5521 agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc aagacatccg
     5581 aatcaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa aaaaggcacc
     5641 cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac
     5701 gccgacgtca ttaccaaagt gaccctggca ccggaaatgg ttcctgcgga agtcatcagc
     5761 aaactggcaa atgccgggat tgtggtttct gccggtcact ccaacgcgac gttgaaagaa
     5821 gcgaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa cgcgatgccg
     5881 tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat
     5941 tgcggtatta tcgctgatgg cctgcatgtt gattacgcca acattcgtaa cgctaaacgc
     6001 cttaaaggcg acaaactgtg tctggttacc gacgccaccg cgccagcagg tgccaacatt
     6061 gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg tgtggatgag
     6121 aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa
     6181 cattgcggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg
     6241 attggcgttg agaaacgtct cggcacactc gccgcaggta aagtagccaa cctgacctca
     6301 ttcacacctg attttaaaat caccaagacc atcgttaacg gtaacgaggt cgtaactcaa
     6361 taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat ctcgtaaaac
     6421 agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc tcgcggattc
     6481 agattgccga gcaaagccag cttgcccccg ccagcgtaac caaaattacg cgtcagctta
     6541 tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc cgccgcgcta
     6601 tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg
     6661 acgccaccat caccctgttt gatctcagca gcaaagtgct ggcagaagaa cattacccgc
     6721 tgccggaacg tacccagcaa acgctggaac atgccctgtt gaatgccatt gctcagttta
     6781 ttgatagcta ccagcgcaaa ctgcgcgagc tgatcgcgat ttcggtgatc ctgccagggc
     6841 ttgttgaccc ggacagcggc aaaattcatt acatgccgca tattcaggta gaaaactggg
     6901 ggctggtaga agctctggaa gaacgtttta aagtgacctg tttcgttggt cacgatatcc
     6961 gtagtctggc gctggcggag cactacttcg gtgcaagtca ggattgcgaa gactccattc
     7021 tggtgcgtgt ccatcgcgga accggggccg ggattatctc taacgggcgc atttttattg
     7081 gccgcaacgg taacgtcggt gaaattggcc atattcaggt cgaaccgctg ggtgaacgct
     7141 gccactgcgg caactttggc tgcctggaaa ctatcgccgc caacgctgcc attgaacaac
     7201 gggtgttgaa tctgttaaag cagggctacc agagccgcgt gccgctggac gactgcacca
     7261 tcaaaactat ctgcaaagcc gcgaacaaag gcgatagtct ggcgtcggaa gtaattgagt
     7321 atgtcggtcg tcatctgggt aaaaccatcg ccattgctat caacttattt aatccgcaaa
     7381 aaattgttat tgccggtgaa atcaccgaag ccgataaagt gctgctccct gctattgaaa
     7441 gctgcattaa tacccaggcg ctgaaggcgt ttcgtactaa tctgccggtg gtacgttctg
     7501 agctggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg
     7561 gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg cttattgttg
     7621 tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct
     7681 gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga ttatggataa
     7741 aggcctgccg ctggtgttgc tgaccaacta tccttcgcag accgggcaag atctggcgaa
     7801 ccgctttgcc actgccggtg tcgatgtgcc ggatagcgtg ttttatacct ctgcgatggc
     7861 gaccgccgat ttcctgcgtc gccaggaagg caagaaagca tatgtggtgg gcgaaggcgc
     7921 actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc ctgatttcgt
     7981 gattgttggc gaaacgcgtt cctacaactg ggacatgatg cataaagcgg cctatttcgt
     8041 cgctaacggt gcacgcttta tcgccaccaa cccggacacc cacgggcgcg gtttttatcc
     8101 cgcttgtggc gcgttgtgtg cagggattga gaaaatctcc gggcgcaaac cgttctatgt
     8161 tggtaagccc agcccgtgga tcatccgcgc agcattaaac aaaatgcagg cgcattcgga
     8221 agaaacggtg attgtcggcg ataacttgcg caccgacatt ctggcgggct tccaggcagg
     8281 tctggaaacg attctggtgc tttctggtgt ttcatcgctc gacgatatcg acagtatgcc
     8341 gttccgccca agctggattt acccgtcagt cgctgaaatc gacgttatct gaataccacc
     8401 cacgtctgcg gacgtggttg gctctgatga caaacgccaa actgcctgat gcgctacgct
     8461 tatcaggcct acgcagctcc tgcaatatat tgaattttca tgcttttgta ggccggataa
     8521 ggcgttcacg ccgcatccgg catgaacaaa tcgcacttta tcagcaattt ggc
//
LOCUS       DAZOZD010000171         8533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOZD010000171 DAZOZD010000000
VERSION     DAZOZD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.171"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHUPB_003219"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHUPB_003219"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0759960.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHUPB_003220"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHUPB_003220"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR0759961.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="ACHUPB_003221"
     CDS             complement(1322..2986)
                     /locus_tag="ACHUPB_003221"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR0759962.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /locus_tag="ACHUPB_003222"
                     /pseudo
     CDS             3028..3861
                     /locus_tag="ACHUPB_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="ACHUPB_003223"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="ACHUPB_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR0759963.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHUPB_003224"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHUPB_003224"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR0759964.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="ACHUPB_003225"
     CDS             8269..>8533
                     /locus_tag="ACHUPB_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0759965.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2390 c         2334 g         1868 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAZOZD010000172         8532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZOZD010000172 DAZOZD010000000
VERSION     DAZOZD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.172"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(171..2162)
                     /gene="tkt"
                     /locus_tag="ACHUPB_003226"
     CDS             complement(171..2162)
                     /gene="tkt"
                     /locus_tag="ACHUPB_003226"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0759966.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2440..3198
                     /gene="loiP"
                     /locus_tag="ACHUPB_003227"
                     /gene_synonym="yggG"
     CDS             2440..3198
                     /gene="loiP"
                     /locus_tag="ACHUPB_003227"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0759967.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3341..4261)
                     /gene="speB"
                     /locus_tag="ACHUPB_003228"
     CDS             complement(3341..4261)
                     /gene="speB"
                     /locus_tag="ACHUPB_003228"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0759968.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4399..6375)
                     /gene="speA"
                     /locus_tag="ACHUPB_003229"
     CDS             complement(4399..6375)
                     /gene="speA"
                     /locus_tag="ACHUPB_003229"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0759969.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6384..6515)
                     /gene="yqgB"
                     /locus_tag="ACHUPB_003230"
     CDS             complement(6384..6515)
                     /gene="yqgB"
                     /locus_tag="ACHUPB_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0759970.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6609..6752)
                     /gene="yqgG"
                     /locus_tag="ACHUPB_003231"
     CDS             complement(6609..6752)
                     /gene="yqgG"
                     /locus_tag="ACHUPB_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0759971.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6863..7114)
                     /gene="yqgD"
                     /locus_tag="ACHUPB_003232"
     CDS             complement(6863..7114)
                     /gene="yqgD"
                     /locus_tag="ACHUPB_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0759972.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7170..8324
                     /gene="metK"
                     /locus_tag="ACHUPB_003233"
     CDS             7170..8324
                     /gene="metK"
                     /locus_tag="ACHUPB_003233"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0759973.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2081 a         2302 c         2187 g         1962 t
ORIGIN      
        1 tataacaaca aatgtcaata cgcatatcgt gatgcatatt acataattga tatttattgc
       61 atataaatat ttgtgatcta caacacgctt tatctattgc ttgtccgcaa acggacatat
      121 caaggtaata aaaaaggtcg ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt
      181 tcttttgctt tcgcaacgac gttgtcgacg gtgaagccga actcttcgaa cagcaactct
      241 gccggagctg actcaccgaa ggtggtcata ccgatgatag cgccattcag gccaacatac
      301 ttgtaccagt agtcagcaat acccgcttct accgcaacgc gtgcagtaac cgctttcggc
      361 agtacggatt cacggtaagc cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa
      421 accacgcgcg ccttcacgcc ttcggcagtc agtttttcgt aggcggcaac agccagttca
      481 acttccgaac cggtcgcgat gaagatcagt tccggctgac cagcacagtc tttcagcaca
      541 taaccaccgc gcgcgatgtt tgccagttgc tcttcagtac gttcctgctg cgccagattc
      601 tgacgggaga ggataagcgc ggtcggaccg tcctgacgct caacaccgta tttccacgcg
      661 accgcggatt caacctggtc acacggacgc catgtagaca tgttcggggt cacgcgcaga
      721 gaagcgacct gctcaaccgg ctggtgagtc gggccgtctt cgcccagacc gatggagtcg
      781 tgggtgtaaa ccatcacctg acgctgtttc atcagcgcag ccatacgtac agcgttacgt
      841 gcgtattcga caaacatcag gaaggtggag gtgtacggca ggaagccacc gtgcagggag
      901 ataccgttag caatcgcggt cataccgaac tcgcgaacac cgtagtggat gtagttaccc
      961 gcagcatctt cgttgattgc tttagaacca gaccacaggg tcaggttaga cggtgccagg
     1021 tcagcggagc cgccgaggaa ttccggcaac agcgggccga acgcttcaat agcattctga
     1081 gacgctttac ggctggcgat tttcgccgga ttagcctgca gtttagcgat aaactctttc
     1141 gctttggcgt cgaagtcaga cggcatttcg cctttcatac ggcgggtaaa ttcagccgct
     1201 tcctgcggat aagctttcgc gtaagccgca aacttctcat tccatgcaga ttctttcgcc
     1261 tggcctgctt ctttcgcatc ccactgcgca tagatttcag acgggatttc gaacggtgcg
     1321 tatttccagc ccagctgttc gcgggtcaga gcgatttctg catcacccag cggcgcaccg
     1381 tgggagtcgt gagtaccggc tttgttcggg gaaccgaaac cgatgatggt tttgcacatc
     1441 agcagggacg gtttgtcagt cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct
     1501 gcgtcatgac cgtcgatgtc gcgaataacg tgccagccgt aagcttcgaa acgcattgcg
     1561 gtgtcgtcgg tgaaccagcc ttcaacgtga ccatcaatag agataccgtt gtcatcgtag
     1621 aacgcaatca gtttacccag cttcagcgta cccgccagag agcaaacttc gtgggagatg
     1681 ccttccatca tgcagccgtc gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg
     1741 cccggacggt taaactgcgc cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg
     1801 gcaataccct gacccagcgg accggtggtg gtttccacac cagcggtata acctacttcc
     1861 gggtggcccg gagttttaga gtgcagctga cggaagtttt tcagttcttc catcggcaga
     1921 tcgtaaccgg tgaggtgcag caggctgtag atcagcatgg agccgtggcc gttggacagc
     1981 acgaagcggt cacggtcagc ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc
     2041 cacaggactt cggcaatgtc agccataccc ataggggcac ccgggtgacc ggatttggct
     2101 ttctgtactg cgtccatgct cagcgcacga atagcattgg caagctcttt acgtgaggac
     2161 attttgactc cagatcggat gatgaagggc acgcccttaa cgacttgacg acagcgcgtt
     2221 ttgggctacg ccggaaaatt tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga
     2281 acatcctttt gccgcttcag aaatctctgg atcatgctcg catgttgcgc aatctactcg
     2341 cccgtccgct gcgcttttcc ttatactgag actgagcgtc gattcacctg caaacggcgc
     2401 atttttagaa taatcctgac cttgtgcgga agagaaaaca tgaaaattcg cgccttattg
     2461 gtagcaatga gcgtggcaac ggtactgact ggttgccaga atatggactc caacggactg
     2521 ctctcatcag gagcggaagc ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc
     2581 ctgagcgatc aggcatgtca ggagatggac agcaaggcga cgattgcgcc agccaatagc
     2641 gaatacgcta aacgtctgac aactattgcc aatgcgctag gcaacaatat caacggtcag
     2701 ccggtgaatt acaaagtgta tatggcgaag gatgtgaacg cctttgcaat ggctaacggc
     2761 tgtatccgcg tctatagcgg gctgatggat atgatgacag ataacgaagt cgaagcggtg
     2821 atcggtcacg agatggggca cgtggcgtta ggccatgtga agaaagggat gcaggtggcg
     2881 cttggtacaa atgccgtgcg agtagctgcg gcctctgcgg gcgggattgt cggcagttta
     2941 tctcaatcac aacttggtga tctgggcgag aaattagtca attcgcaatt ctcccagcgc
     3001 caggaagcag aagccgatga ttattcttac gatcttctgc gccaacgcgg catcagcccg
     3061 gcaggtcttg ccaccagctt tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca
     3121 atgtttgacg accatccggc atccgccgaa cgcgcccagc atattcgcga tcgcatgagc
     3181 gcggatggga ttaagtaaag cctggtggtg tcgaacgtag gtcagataag gcgttcacgc
     3241 cgcatctgac atcaatggca catttgttac cctgtgcgca ttgccggatg cgatgctgac
     3301 tgcatcttat ccggcctacg ggtggcatcg catctggtgc ttactcgccc tttttcgccg
     3361 cttggatata cagcatctcc agcgccagcg tcgccgcagc cagagcggtg atttccgact
     3421 gatcgtatgc cggagccact tccactacgt ccatcccaac aatgttgaga tctttcaggc
     3481 cgcgtaccag tttaatagcg cgatcggagg tcaggccgcc aatcactggc gtaccggtgc
     3541 ctggtgcaaa agcaggatcc aggcagtcga tatcaaaagt caggtaaacc ggcatatcac
     3601 ccacaatctg tttcacttgg gcgataacgt catccacgct gcgatcgttc acctggcagg
     3661 cgtccagcac ggtaaagccg ttgtctttat caaactcggt acgaatacca atctgcacgg
     3721 aatgattcgg gtcgatcaga ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa
     3781 attcacaacc gttcgcatag gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc
     3841 cgaaatgctt cgcatgagca cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg
     3901 tgagcatacg cttaccggca gccagcagct tctcggcgtg cgcctgcagc ttttcgctca
     3961 tctcacgggc atcgccaaag gcatatacca gatcgccgca gtccacgacg ttcagacgct
     4021 cacgcatgtc gaaattccac gggaagcggt tgtgttccca ggccagattc gtcgaaacct
     4081 gacggatcgc tgccggaccg tggcgaccac ccgcacgacc agaagtggcc atatcgaacg
     4141 gcacgccagt aatcacccag tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc
     4201 gtaaaaaacc aaaggcattg gaaaccagtg agttatcgta ttgatgacct aaggtgctca
     4261 tggatggact cctattacaa agtcgatata aaaaaaccct tccgcgtcgt taggcccgac
     4321 gaggaagggt tggatttgtc acaataaatt gtggcggatt atcaccgcta aattaagcgg
     4381 attcaagtaa cacaggactt actcatcttc aagataagta taaccgtaca aacctgcctc
     4441 gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct tcacttgatc
     4501 gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca tgtccgccac
     4561 ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca cgaacacgtc
     4621 aaccgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct gatatgcgcc
     4681 gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca ttggcattgt
     4741 cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt cacaggtaat
     4801 atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg gcagaaccgg
     4861 gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga aattgacgta
     4921 cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg cacggttttg
     4981 cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt ctgcccatgc
     5041 acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt gcagatccat
     5101 ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt gcatttcctg
     5161 ccaggtttcc cacatgcttt gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac
     5221 cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat ggtgcgcagt
     5281 caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt cttcacacgc
     5341 atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt ttacggagca
     5401 gtcagactgc gaacgggtgc cttcatagtc cacgcccaga ccgccgccga cgtcgaagca
     5461 ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc
     5521 cgtggcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca gtagttgcag
     5581 gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc
     5641 caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc cctgcgaagc
     5701 cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt catccagcac
     5761 aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca tcttctcgcc
     5821 aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga tgacgctacg
     5881 ggtcatgcca gcatgtgcca gtactgccat caactcggct ttggaaccgg cttccagacc
     5941 cagcggttcg cccgaatgaa tcagggactc aatcacgcgg cggtgctggt taactttgat
     6001 cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac gtttgaacgc
     6061 ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag
     6121 acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc gagcttccgg
     6181 gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat agtagttatt
     6241 gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg agctcattgc
     6301 aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca aacccataga
     6361 catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca gttaacgact
     6421 atcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag cagtgaatgc
     6481 tgacgctcca actgcgcaac cggtttcttt ttcataacat tattaagcac ataaccgaac
     6541 gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag agcgcccttg
     6601 ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag atgggtataa
     6661 catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc accagaacgg
     6721 tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca cggccgcctg
     6781 ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg gcaacacgaa
     6841 agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc acaatggcag
     6901 aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt tccagccacg
     6961 tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta
     7021 aaatagccat ccagatgtta atccatccat accgattaac actcagactg ccagtgtttt
     7081 taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac agtttgagct
     7141 aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg tccgagtccg
     7201 tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt ttagacgcga
     7261 tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg
     7321 ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag atcacccgta
     7381 acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct aactcctgtg
     7441 cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg
     7501 atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca actaatgaaa
     7561 ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag cgtcaggctg
     7621 aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt
     7681 tccagtatga cgacggcaaa atcgttggta tcgatgctgt cgtgctttcc actcagcact
     7741 ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc atcaagccga
     7801 ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg accggtcgtt
     7861 ttgttatcgg tggcccgatg ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata
     7921 cctacggcgg catggcacgt cacggtggcg gagcattctc tggtaaagat ccatcaaaag
     7981 tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc
     8041 tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa ccgacctcca
     8101 ttatggtaga aactttcggt actgagaaag tgccttctga acaactgact ctgctggtac
     8161 gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg ctgcacccga
     8221 tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg tgggaaaaaa
     8281 ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct
     8341 ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg ataggcattc
     8401 acgccgcatc cggcaaaaag ctgcccgcac aataacatca ttcttcctca acacgtttta
     8461 tcgcaaatta tcatcactac tgaaaccgat tacaccaata acaccagcca aatatttgta
     8521 atattttcat at
//
LOCUS       DAZOZD010000173         8411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOZD010000173 DAZOZD010000000
VERSION     DAZOZD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.173"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            308..1285
                     /gene="glaH"
                     /locus_tag="ACHUPB_003234"
     CDS             308..1285
                     /gene="glaH"
                     /locus_tag="ACHUPB_003234"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0759974.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1305..2573
                     /gene="lhgO"
                     /locus_tag="ACHUPB_003235"
     CDS             1305..2573
                     /gene="lhgO"
                     /locus_tag="ACHUPB_003235"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0759975.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2596..4044
                     /gene="gabD"
                     /locus_tag="ACHUPB_003236"
     CDS             2596..4044
                     /gene="gabD"
                     /locus_tag="ACHUPB_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0759976.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4058..5338
                     /gene="gabT"
                     /locus_tag="ACHUPB_003237"
     CDS             4058..5338
                     /gene="gabT"
                     /locus_tag="ACHUPB_003237"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR0759977.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5649..7049
                     /gene="gabP"
                     /locus_tag="ACHUPB_003238"
     CDS             5649..7049
                     /gene="gabP"
                     /locus_tag="ACHUPB_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0759978.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7070..7732
                     /gene="csiR"
                     /locus_tag="ACHUPB_003239"
     CDS             7070..7732
                     /gene="csiR"
                     /locus_tag="ACHUPB_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0759979.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7733..8182)
                     /gene="kbp"
                     /locus_tag="ACHUPB_003240"
     CDS             complement(7733..8182)
                     /gene="kbp"
                     /locus_tag="ACHUPB_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0759980.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8266..8409)
                     /locus_tag="ACHUPB_003241"
     CDS             complement(8266..8409)
                     /locus_tag="ACHUPB_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0759981.1"
                     /translation="MVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQ
                     TRD"
BASE COUNT         1889 a         2198 c         2357 g         1967 t
ORIGIN      
        1 ggggcagata atatgtttag tttactaacg gtcattttgc agtgaagcca tttactgttt
       61 tttatcgacc agataatctg ttctctaatg ttaactcccc ctaacctgtt gctttagtta
      121 ttcatttcct gtctcacttt gccttaatac tctacgttaa atgttactaa tttgttgctt
      181 ttgatcacaa taagaaaaca atatgtcgct tttgtgcgca tttttcagaa atgtagatat
      241 ttttagatta tggctacgaa atgagcatcg ccatgtcacc ctacatctca taagaggatc
      301 gcttctgatg aatgcactga ccgccgtaca aaataacgct gtcgattcag gccaggacta
      361 tagcggattc actctcatcc cgtcggcgca atccccgcgt ctgctggaac tcaccttcac
      421 cgaacagacg accaaacagt ttctcgagca ggttgccgaa tggcccgtac aggcgctgga
      481 gtacaaatcg tttctgcgtt ttcgggtagg caaaattctt gacgatctgt gtgcgaatca
      541 gctgcaacca ttgctgttga agaccctgct aaaccgtgct gaaggtgcgc tgttgatcaa
      601 tgcggtgggt atcgatgatg tggcgcaggc agatgagatg gtgaagctgg caacggcggt
      661 agcgcatctg attggtcgct caaatttcga tgcgatgagc ggtcagtatt acgcgcgttt
      721 cgtggtgaaa aatgtcgata actcagacag ctatctgcgt cagccgcacc gcgtgatgga
      781 actgcacaac gacggcacgt atgtcgaaga gatcaccgat tacgtgctga tgatgaaaat
      841 cgatgagcaa aacatgcagg gtggaaattc gttgctgctg catctcgatg actgggaaca
      901 tctggaccac tatttccgcc atccactggc gcgtcgcccg atgcgctttg ccgcgccgcc
      961 gagcaaaaac gtcagcaaag atgttttcca tccagtgttc gacgttgatc aacagggtcg
     1021 cccggtgatg cgctatatcg accagttcgt ccagccaaaa gattttgaag aaggcgtgtg
     1081 gttgagcgag ctttccgatg ccattgaaac cagcaaaggc attctttctg tacccgttcc
     1141 cgttggcaaa ttcctgttga taaacaacct gttctggctg cacggtcgcg accgctttac
     1201 tccgcacccg gatctgcgcc gtgaactgat gcgtcagcgt ggctatttcg cttacgccac
     1261 tcaccactac cagacgcatc agtaagcgca aaggaattga gcggatgtat gattttgtga
     1321 ttattggcgg cggcatcatc ggcatgtcga ccgccatgca actgattgat gtttatccgg
     1381 atgcccgcat tgcgttgctg gaaaaagagt ccggcccggc ctgtcaccag acgggccaca
     1441 acagcggcgt gatccatgcc ggggtctatt acacgcccgg cagcctgaag gcgcagtttt
     1501 gcctggcggg gaaccgcgcc accaaagcct tttgcgatca aaacggcatt cgctatgaca
     1561 actgcggcaa gatgctggtc gccacgtctc agctcgaaat ggaacggatg cgcgccttat
     1621 gggaacgcac agcggcgaac ggtatcgagc gcgagtggat aaacgccgtg gaactgcgcg
     1681 agcgcgaacc gaatatcacc gggcttggtg gcatttttgt gccgtccagc ggcattgtca
     1741 gctaccgcga agtcacagcg gcaatggcaa aaatcttcca ggccagaggc ggcgagatta
     1801 tttataacgc cgaagtcagc gccctcagta agcataaaaa cggcgtggtg atacgcaccc
     1861 gtcagggcgg tgaatatgaa gcatcgacgc tgattagctg ttccgggctg atggctgacc
     1921 ggctggtgaa aatgctcggc ctcgaaccgg gctttatcat ctgcccgttc cgtggtgagt
     1981 atttccgcct tgcgccggag cataaccaga ttgttaacca cctgatttac cccattcccg
     2041 accccgcgat gccatttttg ggcgtgcatc tcacccgcat gatcgacggc agcgtgaccg
     2101 tcgggccaaa cgcggtgctg gctttcaaac gcgaaggcta tcgcaagcgc gacttctcat
     2161 ttagtgacac gctggagatt ttgagctcgt cggggattcg ccgggtgctg caaaaccatc
     2221 tacgctcagg actgggcgag atgaaaaact cgctgtgcaa aagcggctac ctgcggctgg
     2281 tgcaaaagta ttgtccccgg ctttcgttaa gcgatctcca gccctggccc gccggtgtgc
     2341 gggcgcaggc agtatcgccg gacggcaagc tgattgacga ttttctgttt gtcaccaccc
     2401 cacgcacgat ccacacctgc aatgcgccct ccccggcagc gacatcagca attcctattg
     2461 gtgcgcatat tgtcagcaag gtacaaacgc tgttggcaag ccagagtaac cccggacgca
     2521 cgctgcgagc ggcacgtagt gtggatgcct tacacgccgc atttaatcaa taacctttga
     2581 aaacaggacg taacaatgaa acttaacgac agtaacttat tccgccagca ggcgttgatt
     2641 aacggggaat ggctggacgc caacaatggc gggatcatcg acgtcaccaa tccggcgaac
     2701 ggcgacaagc tgggtagcgt gccgaaaatg ggcgctgatg aaacccgcgc cgctatcgac
     2761 gccgccaacc gcgccctgcc cgcctggcgt gcgctcaccg ccaaagaacg cgccaacatt
     2821 ctgcgcaact ggttcaattt gatgatggag catcaggacg atttagcgcg cctgatgacc
     2881 ctcgaacagg gcaaaccact ggccgaagcg aaaggcgaaa tcagctacgc tgcctctttt
     2941 attgagtggt ttgccgaaga aggcaaacgt atttatggcg ataccattcc cggtcatcag
     3001 gccgataagc gcctgattgt tatcaagcag ccgattggcg ttaccgcagc tatcacgccg
     3061 tggaacttcc cggcggcgat gattacccgt aaagccggtc cggcgctggc ggcaggctgc
     3121 accatggtgc tgaagcccgc cagtcagaca ccgttctctg cgctggcgct ggcggagctg
     3181 gcgattcgcg cgggcattcc ggctggcgtg tttaacgtag tcaccggttc ggcaggcgcg
     3241 gtcggtaatg aactgaccag caacccgctg gtacgcaaac tgtcgtttac cggttcgacc
     3301 gaaattggcc gccagttaat ggaacagtgc gcgaaagaca tcaaaaaagt gtcgctggag
     3361 ctgggcggca acgcgccgtt tatcgtcttt gacgatgccg atctcgataa agccgtggaa
     3421 ggcgcgctgg cctcgaaatt ccgcaacgcc gggcaaacct gcgtctgcgc caaccgttta
     3481 tacgtgcagg acggcgtgta tgaccgcttt gccgaaaaat tgcagcaggc ggtgagcaaa
     3541 ctgcacatcg gcgacgggct ggataaaggc gtcaccatcg ggccgctgat cgatgaaaaa
     3601 gcggtagcaa aagtggaaga gcatattgcc gatgcgctgg agaaaggtgc gcgtgtggtt
     3661 tgcggcggta aagcggatga acgcggcggc aacttcttcc agccgaccat tctggtggac
     3721 gtgccggcca acgccaaagt gtcgaaagaa gagacgttcg gccccctcgc cccgctgttc
     3781 cgctttaaag atgaagccga tgtgattgcg caagccaatg acaccgagtt tggccttgcc
     3841 gcctatttct acgcccgtga tttaagccgc gtcttccgcg tgggcgaagc gctggagtac
     3901 ggcatcgtcg gcatcaatac cggcattatt tccaatgaag tggccccgtt cggcggcatc
     3961 aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca tcgaagatta cttagaaatc
     4021 aaatatatgt gcatcggtct ttaactggag aatgcgaatg aacagcaata aagagttaat
     4081 gcagcgccgc agtcaggcga ttccccgtgg cgttgggcaa attcacccga ttttcgctga
     4141 ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt gagtatcttg atttcgcggg
     4201 cgggattgcg gtgctcaata ccgggcacct gcatccgaag gtggtggccg cagtggaagc
     4261 gcagttgaaa aaactgtcgc acacctgctt ccaggtgctg gcttacgagc cgtatctgga
     4321 gctgtgcgag attatgaatc agaaggtgcc gggcgatttc gccaagaaaa cgctgctggt
     4381 tacgaccggt tccgaagcgg tggaaaacgc ggtaaaaatc gcccgcgccg ccaccaaacg
     4441 tagcggcacc atcgctttta gcggcgcgta tcacgggcgc acgcattaca cgctggcgct
     4501 gaccggcaag gtgaatccgt actctgcggg catggggctg atgccgggtc atgtttatcg
     4561 cgcgctttat ccttgcccgc tgcacggcat aagcgaggat gacgctatcg ccagcatcca
     4621 ccggatcttc aaaaatgatg ccgcgccgga agatatcgcc gccatcgtga ttgagccggt
     4681 tcagggcgaa ggcggtttct acgccgcgtc gccagccttt atgcagcgtt tacgcgctct
     4741 gtgtgacgag cacgggatca tgctgattgc cgatgaagtg cagagcggcg cggggcgtac
     4801 cggcacgctg tttgcgatgg agcagatggg cgtggcgccg gatcttacca cctttgcgaa
     4861 atcgatcgca ggcggtttcc cgctggcggg cgtcaccggg cgcgcggaag tgatggacgc
     4921 tgttgcaccg ggcggtctgg gcggcaccta tgccggtaac ccgattgcct gcgtggctgc
     4981 gctggaagtg ttgaaggtgt tcgagcatga aaatctgctg caaaaagcca acgatctggg
     5041 gcagaagcta aaagacggat tgctggcgat agccgaaaaa cacccggaga tcggcgacgt
     5101 acgcgggctg ggagcgatga ttgccatcga gctgtttgaa gatggcgatc acagcaaacc
     5161 ggacgcaaaa ctcaccgccg agatcgtggc acgcgcccgc gataagggcc tgattcttct
     5221 ctcctgcggc ccgtattaca acgtgctgcg catccttgta ccgctcacca ttgaagacgc
     5281 tcagatccgt cagggtctgg agatcatcag ccagtgtttt gctgaggcga agcagtagct
     5341 atgtccgttt gtaagagtta accgttgcta agggaatgat aacgatgcag taaaaaataa
     5401 taacgatgag gcgaacgaca aagttaaaaa cgactgcgga cagtcccctc gcccctctgg
     5461 agagagggtt agggtgaggg gtaaaacacg cggctaaacc ctcccctggc cctctcccta
     5521 agggagaggg agaaaaccct cccgctttcg tttttaagat tttgttgacc gtctcaatta
     5581 aacgcgtgac ccggcgcggg aaatgtcggg gcgctctccc aagtgacaca ctttcgagag
     5641 gattcaggat ggggcaatca tcgcaaccac atgagttagg cggcgggctg aaatcacgcc
     5701 acgtcaccat gttgtctatt gccggtgtta tcggcgcaag tctgtttgtc ggttccagcg
     5761 tcgccatcgc cgaagcgggc ccggcggtat tactggccta tctgttcgcc gggctactgg
     5821 tggttatgat tatgcggatg ttggcggaaa tggcggttgc cacgcccgat accggttcgt
     5881 tttccaccta tgccgataaa gccattggtc gctgggcggg ctataccatc ggctggttgt
     5941 actggtggtt ttgggtactg gttatcccgc tggaagccaa tatcgccgct atgatcctgc
     6001 actcgtgggt tccaggcatt cccatctggt tattttccct cgtcattacc cttgccttaa
     6061 ctggcagtaa tttattaagc gttaaaaact acggcgaatt tgagttctgg ctggcgctgt
     6121 gcaaagtcat cgctatcctg gcctttattt tccttggtgc agtcgcaatt agcggttttt
     6181 acccttatgc cgaagtgagc gggatctcaa gattgtggga tagcggcggc tttatgccca
     6241 acggtttcgg tgcggtatta agcgcgatgt tgatcaccat gttctcgttt atgggcgcag
     6301 aaattgtcac cattgccgcc gcggaatccg acacgccgga aaaacatatt gtccgcgcca
     6361 ccaactcggt tatctggcgt atttctatct tctatttgtg ctctattttt gtcgtcgtgg
     6421 cattaatacc gtggaatatg ccggggctga aagccgttgg atcttatcgc tcggttctgg
     6481 aattgctcaa tattccccat gcgaaattaa tcatggactg tgtgatatta ctttccgtaa
     6541 ccagttgcct gaactcggcc ctgtataccg cctcaaggat gctctactcc ttaagccgtc
     6601 gcggtgatgc tcccgcggta atgggtaaaa tcaaccgcag taaaaccccg tacgtggcgg
     6661 tgttactctc caccggcgcg gcattcttaa cggtggtggt gaactattac gcgcctgcga
     6721 aggtatttaa atttctgatc gacagctccg gcgctatcgc cctgctggtt tatttagtca
     6781 tcgccgtttc acagttgcgg atgcgcaaaa ttctgcgagc ggaaggaagc gaaattcgct
     6841 tgcgaatgtg gctttatccc tggctcacct ggctggtcat cggctttatt acctttgtgt
     6901 tggtagtgat gctattccgt ccggcgcaac agttagaagt gatctccacc ggcttattag
     6961 cgatagggat tatctgtacc gtgccaatta tggctcgctg gaaaaagctg gtattgtggc
     7021 aaaaaacacc cgttcataat acgcgctgat aatgatcagg agtcacacca tgaccattac
     7081 gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga
     7141 tgaaaaatta cggatgagtt tgctgacatc gcgttatgca cttggcgttg ggccgttgcg
     7201 ggaagcactt tctcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta
     7261 tcgggtggcg tctatgtcag agcaggagct gctcgatatt ttcgatgccc gcgccaatat
     7321 ggaagcgatg ttagtgagtc tggcgattgc ccgcggtggc gatgagtggg aggcagacgt
     7381 tctcgcaaaa gcgcatctgc tcagtaagct ggaagcctgt gacgccagcg agaaaatgct
     7441 tgatgagtgg gatctgcgtc atcaggcgtt tcatacagca attgtggcgg gctgtggttc
     7501 tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgct atcgatttat
     7561 ctggctgcgg cgaacggtgc tttcggtaga aatgctggag gataaacacg atcagcacca
     7621 gaccctgact gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca
     7681 gcatttactg acgccaattc ccattatcca gcaggcgatg gctggcaatt aactactctt
     7741 ccggaatacg caacacttgc cccggataaa ttttatccgg gctttttagc atcggtttat
     7801 tcgcttcgaa gattttattg tacagattag cgttaccgta gacctgtttg gaaatggcac
     7861 tcagagtgtc gccagactta acggtataaa actggctggc agtggctggt gtcgccgttt
     7921 tcacctgatc atcaacactg gcaataccgg aaatattccc caccgcaaca aggattttct
     7981 ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa
     8041 tgttcacttt atcagcatca ggtataccgg ttttgctcag atgctcctgc accttcttcg
     8101 cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct ccggcatctt
     8161 tcacaaaatt gaacagaccc atagctacct cattcgttaa cggaaacact cagaaagtgt
     8221 agcagaggaa cgcggcgcgt taacgccgcg ggggtgagca caggtttaat cgcgggtttg
     8281 cacccagaac gcgtgaatca aaccggggat atacccaagc agtgtcagca gaatattaat
     8341 aatgaacgcc caaccgaacc ctttaccgag cagcacgccg agcggcggta gaatgatggt
     8401 gatgacgatt c
//
LOCUS       DAZOZD010000174         8409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZOZD010000174 DAZOZD010000000
VERSION     DAZOZD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.174"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            125..736
                     /gene="ruvA"
                     /locus_tag="ACHUPB_003242"
     CDS             125..736
                     /gene="ruvA"
                     /locus_tag="ACHUPB_003242"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0759982.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            745..1755
                     /gene="ruvB"
                     /locus_tag="ACHUPB_003243"
     CDS             745..1755
                     /gene="ruvB"
                     /locus_tag="ACHUPB_003243"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0759983.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1902..2687)
                     /gene="znuB"
                     /locus_tag="ACHUPB_003244"
     CDS             complement(1902..2687)
                     /gene="znuB"
                     /locus_tag="ACHUPB_003244"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0759984.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2684..3439)
                     /gene="znuC"
                     /locus_tag="ACHUPB_003245"
     CDS             complement(2684..3439)
                     /gene="znuC"
                     /locus_tag="ACHUPB_003245"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0759985.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3518..4450
                     /gene="znuA"
                     /locus_tag="ACHUPB_003246"
     CDS             3518..4450
                     /gene="znuA"
                     /locus_tag="ACHUPB_003246"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0759986.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4466..5788
                     /gene="mepM"
                     /locus_tag="ACHUPB_003247"
     CDS             4466..5788
                     /gene="mepM"
                     /locus_tag="ACHUPB_003247"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0759987.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5908..6879
                     /gene="lpxM"
                     /locus_tag="ACHUPB_003248"
                     /gene_synonym="msbB"
     CDS             5908..6879
                     /gene="lpxM"
                     /locus_tag="ACHUPB_003248"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0759988.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6967..8409)
                     /locus_tag="ACHUPB_003249"
                     /pseudo
     CDS             complement(6967..8409)
                     /locus_tag="ACHUPB_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2257 a         1984 c         2087 g         2081 t
ORIGIN      
        1 tatactgtgc catttttcag ttcatcgaga cacctcgcaa gttttcttca tccttcgctg
       61 gatatctatc cagcattttt ttatcataca gcattatctt tgattcatta cgcaggagcg
      121 tcatgtgata ggcagactca gaggcatcat cattgaaaaa caacccccgc tggtgttaat
      181 tgaagtgggc ggcgtaggct atgaagtgca tatgccgatg acctgttttt atgaactccc
      241 tgaagcgggt caggaagcga tcgttttcac ccactttgtg gtgcgtgaag acgcgcaact
      301 gctgtacggt tttaacaata aacaagagcg cacgttgttt aaagagttaa tcaaaaccaa
      361 cggcgtcggc ccgaagttgg cgctggcgat cctctccgga atgtcagcgc agcagttcgt
      421 taatgccgtt gaacgtgaag aagtgggagc actggtgaaa ctgccgggca ttggtaaaaa
      481 aaccgccgag cgtttaattg tcgaaatgaa agaccgattt aaaggtttgc acggcgatct
      541 ctttacgcca gccgccgatc tggtgctcac gtcgcctgcc agcccggcaa ccgacgatgc
      601 tgaacaggaa gcggttgccg cgctggtggc gctgggctat aaaccacaag aagcaagccg
      661 catggtgagc aaaatcgctc gcactgacgc cagcagtgaa acattaattc gcgaagccct
      721 acgcgccgcg ttatgaggta aaggatgatt gaagcagacc gtctgatttc tgccggtacc
      781 actttgccgg aagatggggc agatcgcgcc attcgcccta aattactgga agagtatgtt
      841 ggtcagccgc aggttcgttc acagatggag attttcatca aagcagcgaa actgcgcggc
      901 gatgccctcg atcatctgtt gatttttggt cctccggggt tgggtaaaac tacgcttgcc
      961 aacattgtcg ccaatgaaat gggcgttaat ttacgcacga cttctggtcc ggtgctggaa
     1021 aaggcgggcg atctggcagc gatgctcact aaccttgaac cgcatgacgt gctgtttatt
     1081 gatgagatcc accgtctatc gccagttgtt gaagaagtgc tgtacccggc aatggaagac
     1141 taccaactgg atatcatgat tggtgaaggt ccggcggcac gctccattaa aattgatttg
     1201 ccgccgttta ccctgattgg tgcaaccacg cgcgcaggtt cgctgacatc accgttgcgc
     1261 gaccgttttg gtattgtgca acgtctggag ttttatcagg tgccggatct gcaatatatc
     1321 gtcagtcgca gcgcacgctt tatggggctt gagatgagtg atgacggcgc gctggaagtt
     1381 gctcgtcgcg ctcgcggtac gccacgtatt gccaaccgtc tgctgcgtcg agtgcgtgat
     1441 ttcgccgaag tgaagcacga tggcaccatc tcggcagata tcgctgctca ggcgctggat
     1501 atgttgaatg tcgatgctga aggtttcgat tatatggacc gcaaattgtt gctggcggta
     1561 atcgataagt tctttggtgg gccggtaggt ctggataacc tggcggcagc cattggcgaa
     1621 gaacgtgaaa ccattgagga tgtgctggag ccttatctga ttcagcaagg ctttttgcag
     1681 cgtacaccgc gtgggcgtat ggcgacgacg cgggcgtgga atcactttgg cataacgccg
     1741 ccagaaatgc cgtaagtcgg attggtcgct cgcaccgcat ccgacattgg cagtgtgcct
     1801 gatgcgacgc tattcgcgtc ttatccggcc taccagagat agcgaaccgt aggtcggata
     1861 aggcgctcgc gccgcatccg acaaatgtgt tcagcgacag attagctggc ctgctttttc
     1921 atcatactga taataaataa cagtgcagca catagcacta ccgacggacc cgccggcgta
     1981 tcgtaaaacg cggaaaaggt taaaccgcca gttactgcca ccatccccac caaaacagcg
     2041 acaccagcca tctgttccgg cgtacgggca aagcgacgcg cagtagctgc aggaataatc
     2101 agcaacgaag taataatcaa cgcaccgaca aatttcatcg ctacaccaat cgtcaatgcc
     2161 gtcaccagca tcaacaacaa tttcacgcgc tgtaatttca caccatcaac aaacgccaga
     2221 tccgggctaa tcgtcatcga cagcaaattg cgccattgcc agaacaaaat agccaccacg
     2281 atgaccacgc caatcgcaat agagatgaga tcttctggcg tcactgccag caaatcaccg
     2341 aacaggtaag ccatcaaatc aacacgaata ttagacatca gactaacgac caccaggccc
     2401 agcgacaggg cactgtgcgc cataatccct aataacgtgt cgatcgccag ctgtggacgc
     2461 ttctccagcc ataccagacc gcccgccagc agcagcgtaa cggcaatcac cgcatagaat
     2521 ggattcacgt ccagcaacaa accaaacgcg acaccaagta atgaggcatg agccagcgta
     2581 tcaccgaaat aagacatacg acgccagact acaaacgaac ccagcggacc cgcggcacag
     2641 gcgagcatga tcccggctaa ccaaccggga aataataatt caatcatgag cgatcatttc
     2701 cccgacgcaa aacaattcgt ccctgtaaat cgtgacgatg gttatgatga tggcgataga
     2761 tacccagttg ttcagcacca cgaggaccaa acattgaaat aaactccgga tgcagggaaa
     2821 caacttccgg tgtgccggaa caacaaatgt ggtgattcag gcacagcact tcatcggttt
     2881 ttgccatcac cagatgcaga tcgtgagaaa ccattaaaac gccacaatcc agttcacgac
     2941 gcagttggtc aataaggtca tataacgcca cctggccatt tacatccacg ccttgagtgg
     3001 gttcatccag cactaataat tgcgggcgat ttaacaatgc tcgcgctaac agtacacgct
     3061 gcgtttcgcc accagagagc ttttgcatcg gtgcgttaat caggtgcccg gcctggacac
     3121 gtttcagtgc aggcaaaata tcttctttat gtgtaccagg gcgtaagcgt aaaaaacggt
     3181 ttacggtcag tggcaacgtg gtgtcgagat acagcttctg cggtacatag ccgatgcgca
     3241 gttttccgtt gcgcttgata accccttcat cgggtgttac cagcccgagc actacccgta
     3301 ccagtgtcga cttacctgcg ccatttggcc caagtaaagt caaaattttt ccaggtttaa
     3361 gttccagcga cacatcagag aggacgcggc gttggccaaa agaaaccgag acattttcca
     3421 aggaaaccag acttgtcatg ttaattttag tcttgcagta gtcatgaaat gttataatat
     3481 cacacttctc atattcatta cgatgattgg tcgcattatg ttacataaaa aaacgcttct
     3541 tttcgcagca ttatccgccg ctctctgggg aggtgcaaca caggccgcag atgccgccgt
     3601 tgtcgcttcg cttaaacccg ttgggttcat cgcttctgcc attgctgatg gggtaacaga
     3661 aacagaggtt ttacttcctg acggcgcttc agaacatgat tattcactgc gcccatcgga
     3721 tgtaaaacgc ttacagaacg cggacttagt cgtttgggtt ggcccggaga tggaagcgtt
     3781 tatgcaaaaa ccggtaagca aattaccagg agcgaagcag gtaacgattg cgcagcttga
     3841 agatgtgaaa ccgctactga tgaaaagtat tcacggcgat gatgatgatc acgaccacgc
     3901 ggaaaaaagt gacgaagatc accatcacgg cgatttcaac atgcatcttt ggctttcccc
     3961 agagatagcg cgggctacag cggttgcaat ccatggaaaa ttagtggaac ttatgccgca
     4021 aagtcgagcc aaacttgacg ccaacctgaa ggattttgag gcacaattag cctcaaccga
     4081 aacgcaggtt ggtaacgagc tcgcgccgct caaggggaaa ggttatttcg tttttcacga
     4141 tgcttacggc tatttcgaaa aacagttcgg actgacaccg cttggtcatt ttaccgttaa
     4201 ccctgagatt caacctggcg cgcagcgttt acatgaaata agaacacagt tggttgagca
     4261 aaaagcaacc tgcgtttttg ctgagccaca gttcaggcca gcggtcgttg agagcgtcgc
     4321 acgagggaca tccgttcgta tgggaacgtt ggatcccctg gggacgaata tcaaactggg
     4381 taaaacaagc tattcagaat tcctgagtca attagccaac cagtatgcga gctgcctgaa
     4441 aggagattaa tgaggaagtg attacgtgca acagatagcc cgctctgtcg ccctggcgtt
     4501 taataattta ccgcgaccac accgcgttat gttggggtcg ctcaccgttc ttactctggc
     4561 cgtcgctgtc tggcggccct atgtttatca ccgcgatgcc acgccaattg tcaaaaccat
     4621 tgagctggaa cagaacgaaa ttcgttcgct cttacctgaa gccagtgagc cgattgatca
     4681 agctgcacaa gaagatgaag ccattcccca ggacgaactg gatgacaaaa tcgccggtga
     4741 agcgggcgtg catgaatatg ttgtttccac tggcgatacg ctaagcagca ttctcaatca
     4801 gtatggtatt gatatgggtg atatcaccca actggctgcg gccgacaaag aattgcgtaa
     4861 cctgaaaatc ggtcaacaac tctcctggac attaaccgcg gacggcgaac tgcagcgcct
     4921 cacctgggaa gtgtctcgtc gtgaaacccg aacctatgac cgtactgccg ctaacggttt
     4981 taaaatgacc agcgaaatgc agcaaggaga gtgggttaac aatctgctga aaggtaccgt
     5041 cggggggagc tttgttgcca gcgccagaaa cgccggttta accagcgccg aagtgagcgc
     5101 agtgattaaa gccatgcagt ggcaaatgga tttccgcaaa ctgaaaaaag gcgatgaatt
     5161 tgcggtgtta atgtctcgag aaatgcttga tggtaaacgt gagcaaagcc agctgctggg
     5221 cgtacgtttg cgttcagaag gtaaagatta ttacgctatc cgcgctgaag atggcaaatt
     5281 ctacgatcgt aacggtactg gtctggcgaa aggattcttg cgattcccga cggcgaaaca
     5341 gttccgtatc tcgtctaact ttaacccgcg tcgtactaat ccggtgaccg gtcgcgttgc
     5401 agcacacaga ggtgttgatt tcgccatgcc gcagggtacg ccagtgcttt cagtgggtga
     5461 cggtgaagtg gtggttgcca aacgcagtgg cgcagcaggt tattatgtgg ctattcgtca
     5521 tggtcgcagc tacaccacgc gttatatgca cttgcgcaag attctggtga aaccgggaca
     5581 gaaggtgaaa cgtggcgacc gtatcgcgct ttccggtaat accggacgtt caaccgggcc
     5641 gcatctgcac tatgaagtat ggataaacca gcaggccgta aacccgctga cggcaaaact
     5701 gccgcgtacc gaagggctga ccggctccga tcgtcgcgaa ttcctggcgc aggccaaaga
     5761 gattgtgccg cagctacggt ttgattaatt aacatccatt cgcagccggt acgcagtcag
     5821 tatcggcttt ttttatttgg tgcggggcaa gttgcgccgc tatactatca ccagattgat
     5881 ttttgcctta tccgaaactg gaaaagcatg gaaacgaaaa aaaataatag cgaatacatt
     5941 cctgagtttg ataaatcctt tcgccacccg cgctactggg gagcatggct gggtgtagca
     6001 gcgatggcgg gtatcgcttt aacgccgcca aagttccgtg atcccattct ggcacggctg
     6061 ggacgttttg ccggacgact gggaaaaagc tcacgccgtc gtgcgttaat caatctgtcg
     6121 ctctgctttc cagaacgtag tgaagctgaa cgcgaagcga ttgttgatga gatgtttgcc
     6181 accgcgccgc aagcgatggc aatgatggct gagttggcaa tacgcggacc ggagaaaatt
     6241 cagccgcgcg ttgactggca agggctggag atcatcgaag agatgaggcg taataacgag
     6301 aaagttatct ttctggtgcc gcacggttgg gccgtcgata ttcctgccat gctgatggcc
     6361 tcgcaagggc agaaaatggc agcgatgttc cataatcagg gcaacccggt ttttgattat
     6421 gtctggaaca cggtgcgtcg tcgctttggc ggtcgtctgc atgcgagaaa tgacggtatt
     6481 aaaccattca tccagtcggt acgtcagggg tactggggat attatttacc cgatcaggat
     6541 catggcccag agcacagcga atttgtggat ttctttgcca cctataaagc gacgttgccc
     6601 gcgattggtc gtttgatgaa agtgtgccgt gcgcgcgttg ttccgctgtt tccgatttat
     6661 gatggcaaga cgcaccgtct gacgattcag gtgcgcccac cgatggatga tctgttagag
     6721 gcggatgatc atacgattgc gcggcggatg aatgaagaag tcgagatttt tgttggtccg
     6781 cgaccagaac aatacacctg gatattaaaa ttgctgaaaa ctcgcaaacc gggcgaaatc
     6841 caaccgtata agcgtaaaga tctttatcct atcaaataaa aaaagcctct caaattattg
     6901 agaggctgta ctcgataaaa aatctcaatg agtgttccca ctattttgag tttttaggtt
     6961 agtacgctat tgagttaatc gtagcgtgat aatttcaaaa ggtttgaact caagattgca
     7021 atattgattg caataaaaac tgtcatctaa cttattttct tgaagatcga catattgaac
     7081 gtagtgataa ttacatactc acgaaagcat ggctgaaatt tctaatccat gactctcata
     7141 taatcttacg attataccgt cgccgtcttc agctaatttt attgtttcga tgataacgct
     7201 gttagtatcg atagcaaaaa atgatagtga agggggtaat tcacccgggt gcggtttgac
     7261 agggacgacc tgtaagggat gattcattat ataggcttga ttaataacac cacccacacg
     7321 gtaatctcca tgatgaggaa ataaggagaa tatgaatttt tgtttaccaa tatctgcaaa
     7381 attattaatc aggaaattat cataattgtc tgtcttaaca acttccccag gtttatgctg
     7441 cgaacgcagc aaagttaatt caagcgtctg gtctttgaca cgatagccat atttgttttt
     7501 tgctattaaa gctatgccag tttctcgatc agaaaggtcg acccagtgat gtgctggtat
     7561 ttcatctttg gcaaaagcga acgaatcatc gctatgcgtt ggtctgtcaa ttacaccgaa
     7621 ctgaatttga cattgtgcaa gagatgtttg tactgacacc gggaatttta ctttgagaga
     7681 aacattaggt tcgtgccaat caataaacgt ttggacatca acgcgtggac tatcctgtgt
     7741 cagggtaaag gctaaacgaa gataagagga attgtagtga taaatcagcg tcatcgtagt
     7801 tttaggcccg ctgatcacag ttgtcatttt ttcagcgagt aaaacatatg attccccatc
     7861 acgataattt gaagcaaaat cccaggccgc gaaaaaacct gcatcttcgt ggtatgcacg
     7921 gatctcactg tgcatatttt cagtaatgta ttctttacca tatcttttat catatagcga
     7981 aattaaatca ccgctttctg aaaaaagaag tttaatgtaa ttattttcaa tactttgaga
     8041 ttctgccttc agaccattta cgatcttatt ctttggaaca atcacagcat aaccataaga
     8101 gtttactcgt gctttgagcc agttgttgtt tatattgatc cattcgttac gagcaaaaca
     8161 ggtggtatta aacaaaatgt gcggatcttt ataagaagaa gtgtcaattt tacttaataa
     8221 agtagactgc gcatcagaaa taattttctc ggtttttgct tctagtttaa gatattcctt
     8281 ttcagtttca tgataaactc ttgaaataca tgagccggga aggatgtcat ggaattgcaa
     8341 ggtcagcatc gctttccata tttcatcaaa ctcactgcga agaattgatg tcatattatt
     8401 agtaatagt
//
LOCUS       DAZOZD010000175         8407 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOZD010000175 DAZOZD010000000
VERSION     DAZOZD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.175"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            85..225
                     /locus_tag="ACHUPB_003250"
     CDS             85..225
                     /locus_tag="ACHUPB_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0759989.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            228..395
                     /gene="yciX"
                     /locus_tag="ACHUPB_003251"
     CDS             228..395
                     /gene="yciX"
                     /locus_tag="ACHUPB_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0759990.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            509..604
                     /gene="ymiC"
                     /locus_tag="ACHUPB_003252"
     CDS             509..604
                     /gene="ymiC"
                     /locus_tag="ACHUPB_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0759991.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            768..3443
                     /gene="acnA"
                     /locus_tag="ACHUPB_003253"
     CDS             768..3443
                     /gene="acnA"
                     /locus_tag="ACHUPB_003253"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0759992.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3507..4097)
                     /gene="ribA"
                     /locus_tag="ACHUPB_003254"
     CDS             complement(3507..4097)
                     /gene="ribA"
                     /locus_tag="ACHUPB_003254"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0759993.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4267..5031
                     /gene="pgpB"
                     /locus_tag="ACHUPB_003255"
     CDS             4267..5031
                     /gene="pgpB"
                     /locus_tag="ACHUPB_003255"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0759994.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5180..5488
                     /gene="lapA"
                     /locus_tag="ACHUPB_003256"
     CDS             5180..5488
                     /gene="lapA"
                     /locus_tag="ACHUPB_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0759995.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5495..6664
                     /gene="lapB"
                     /locus_tag="ACHUPB_003257"
     CDS             5495..6664
                     /gene="lapB"
                     /locus_tag="ACHUPB_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0759996.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6857..7594
                     /gene="pyrF"
                     /locus_tag="ACHUPB_003258"
     CDS             6857..7594
                     /gene="pyrF"
                     /locus_tag="ACHUPB_003258"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0759997.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7594..7920
                     /gene="yciH"
                     /locus_tag="ACHUPB_003259"
     CDS             7594..7920
                     /gene="yciH"
                     /locus_tag="ACHUPB_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0759998.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8046..8264)
                     /gene="osmB"
                     /locus_tag="ACHUPB_003260"
     CDS             complement(8046..8264)
                     /gene="osmB"
                     /locus_tag="ACHUPB_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0759999.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         2078 a         1971 c         2221 g         2137 t
ORIGIN      
        1 actaaagttt ctttaggatt tgtctcatcg tatgattaat tacaccaatc gttcaacgtc
       61 ttgctgataa gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc
      121 cgccgcgatc ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt
      181 ttctgggtgg ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg
      241 agcaactgga gtcgtcacca ttatgccagc atagtgacaa tgaaacagaa acgaaacggg
      301 aatgttccgt cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag
      361 aactgtttgc tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt
      421 ttaatgaatt aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac
      481 aaaataataa ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg
      541 gatgggcgcg ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca
      601 ctgatccttg accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct
      661 tttatcaatt tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca
      721 ccctgaagag aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc
      781 tacgagaagc cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc
      841 tgccgcttgc tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt
      901 tgctcgaaaa cctgctgcgc tggcaggatg gtaactcggt taccgaagag gatatccacg
      961 cgctggcagg atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa
     1021 gggtgttgat gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg
     1081 aagcggttaa acgcctcggc ggcgatactg caaaggttaa cccgctctca ccggtcgacc
     1141 tggtcattga ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa
     1201 acgtacgcct ggaaatggag cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc
     1261 aagcgttcag tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc
     1321 tcgaatatct cggcaaagca gtgtggagtg aattgcagga cggtgaatgg attgcttatc
     1381 cggatacact cgttggtact gactcgcaca ccactatgat caacggcctt ggcgtgctgg
     1441 ggtggggcgt gggtgggatc gaagcagaag ccgcaatgtt aggccagccg gtttccatgc
     1501 ttatcccgga cgtagtgggc tttaaactta ccggtaaatt acgtgaaggt attaccgcca
     1561 cagacctggt tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg gagaaattcg
     1621 tcgaatttta tggtgatggt ctggattcac taccgctggc ggatcgcgcc accattgcca
     1681 atatgtcgcc agaatatggt gccacctgtg gcttcttccc aatcgatgct gtaaccctcg
     1741 attacatgcg tttaagcggg cgcagcgaag atcaggttga gttggtcgaa aaatatgcca
     1801 aagcgcaggg catgtggcgt aacccgggcg atgaaccgat ttttaccagt acgttagaac
     1861 tggatatgaa tgacgttgaa gcgagcctgg cagggccaaa acgcccacag gatcgcgttg
     1921 cactgcccga tgtaccaaaa gtatttgccg ccagtaacga actggaagtg aatgccacgc
     1981 ataaagatcg ccagccggtc gattatgtta tgaacggaca tcagtatcag ttacctgatg
     2041 gcgctgtggt cattgctgcg ataacctcgt gcaccaacac ctctaaccca agtgtgctga
     2101 tggctgcagg cttgctggcg aaaaaagccg taattcttgg cctcaagcgg caaccgtggg
     2161 tcaaagcgtc gctggcacca ggctcgaaag ttgtttctga ctatctggca aaagcgaaac
     2221 tgacaccgtt tctcgacgaa ctggggttta accttgtggg atacggttgt actacctgta
     2281 ttggtaactc tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa
     2341 ccgtcggtgc agtgctgtcc ggcaaccgta actttgaagg ccgtatccat ccgctggtta
     2401 aaaccaactg gctggcctcg ccgccgctgg tggttgccta tgcgctggcg ggaaatatga
     2461 atatcaacct ggcttctgag cctattggtc atgatcgtaa aggcgatccg gtttatctga
     2521 aagatatctg gccatcggca caagaaattg cccgtgcggt agaacaagtc tccacagaaa
     2581 tgttccgcaa agagtacgca gaagtttttg aaggcacagc agagtggaag gaaattaacg
     2641 tcacacgatc cgatacctac ggttggcagg aggactcaac ctatattcgc ttatcgcctt
     2701 tctttgatga aatgcaggca acaccagcac cagtggaaga tattcacggt gcgcggatcc
     2761 tcgcaatgct gggggattca gtcaccactg accatatctc tccggcgggc agtattaagc
     2821 ccgacagccc agcgggtcga tatctacaag gtcggggtgt tgagcgaaaa gactttaact
     2881 cctacggttc gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc gccaatattc
     2941 gcatccgtaa tgaaatggtg cctggcgttg aaggggggat gacgcggcat ttacctgaca
     3001 gcgacgtagt ctctatttat gatgctgcga tgcgctataa gcaggaacaa acgccgctgg
     3061 cggtgattgc cgggaaagag tatggttcag gctcaagccg tgactgggcg gcaaaaggtc
     3121 ctcgtctgct tggaattcgt gtggtgattg ccgaatcgtt tgaacgaatt caccgttcga
     3181 atttaattgg catgggcatc ctgccgctgg aatttccgca aggcgtaacg cgtaaaacgt
     3241 taggactaac cggggaagag aagattgata ttggcgatct gcaaaatcta caacccggtg
     3301 cgacggttcc ggtgacgctt acgcgcgcgg atggtagcca ggaggtcgta ccctgccgtt
     3361 gtcgtatcga caccgcgacg gagttgacct actaccagaa cgacggcatt ttgcattatg
     3421 tcattcgtaa tatgctgaag taacaaatat ttgcttgccg gttattttgc ttccggcaag
     3481 caaattaatt ccacaatgcg agagggttat ttgttcagca aatgacccat tttctcggct
     3541 ttggtatcga gataatgttc gttattgggg ttacgaccta caatcaatgg tacgcgttca
     3601 acaatattaa tccctgcttc ggtcagaatt tcgacttttt tcgggttatt ggttaacaag
     3661 cggacttcat tgacgccaag gagtttgaac atatcagcgc aaagagtgaa gtcgcgctca
     3721 tcagcggcga agcctaactg atggttagcc tctacggtat cgtaaccttg atcctgcagt
     3781 gcgtaagcgc ggattttatt cagcagacca atgttacgac cttcctgacg gtgatacagc
     3841 aaaataccac ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa
     3901 tcgcagcgca agctgaacag ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt
     3961 accggggtat gcccggaaat atcgccatag actagcgcga catgatcgtg tccggttgcc
     4021 agttcttcaa atcccaccat caggaaatcg ccccatgggg ttggcagttt ggcttctgcc
     4081 acacgtttaa gctgcatgaa attctccaga taatgctggt tctgtattgg cttattttgc
     4141 cataacgaga aggggttcac ctaatcacga cgcgtcgatc gttcacggaa tggcacaaat
     4201 ctgtcaattt ttcctggaac tggcgttttc agttatgatt gtgggactta tcaaaaagga
     4261 gaggccatgc gttcgattgc cagacgtacc gcagtgggag ctgcactatt gcttgtcatg
     4321 ccagtagccg tatggatttc tggctggcgt tggcaacctg gagaacaaag ttggctacta
     4381 aaagcggctt tttgggttac tgaaactgtc acccagccct ggggcgtcat tacacatttg
     4441 attttattcg gctggtttct ctggtgtctg cgtttttgca ttaaggctgc ctttgtatta
     4501 tttgccattc tggcggccgt aatcattgtg ggacaaggcg ttaaatcctg gatcaaagac
     4561 aaagtccagg aaccacgacc ttttgttatc tggctggaaa aaacacatca tattccggtt
     4621 gatgagttct acactttaaa gcgagcagaa cgcggaaatc tagtgaaaga acagttggct
     4681 gaagagaaaa atatcccaca atatttgcgt tcacactggc agaaagagac ggggtttgcc
     4741 tttccttccg gtcacacgat gtttgctgcc agttgggcac tgctggccgt tggtttgctg
     4801 tggccgcgtc ggcgaacgtt aaccattgct atcttgctgg tctgggcaac gggagtcatg
     4861 ggaagccgcc tgctgctcgg gatgcattgg ccacgcgatc tggtagtagc tacgttgatt
     4921 tcgtgggcgc tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg gccattaaca
     4981 ccacctgcgg aagaaaatcg cgaaatagcg caacgagaac aagaaagtta acgctggttg
     5041 attttccgaa tttagccctt aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg
     5101 cgcgaaaatg gtactttaaa gggctattgc ggtaagttga ccataattta ttcgctttaa
     5161 ccacataacg ggaagtaatg tgaaatattt actcattttc ttactggtgt tagcgatctt
     5221 cgtgatttcg gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta attatctgtt
     5281 agcgcaaggg gagtaccgta tttccacatt gctggcggta ttgtttgctg cgggggttgc
     5341 tatcggttgg ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc
     5401 tgaacgtaaa ataaagcgac tggaaaacca gctttcaccc gcgactgacg tggctgtagt
     5461 gccgcactcg tcagcggcga aggaataact ttctatgctg gagttgttgt ttctgctttt
     5521 gcctgtagcc gctgcctatg gctggtatat gggccgcaga agtgcgcaac aaaacaagca
     5581 agatgaagcc aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc tgcttagtaa
     5641 tcaacaggat aaagcggtag acctgtttct cgatatgctt aaagaggata caggcaccgt
     5701 tgaagcccac cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat
     5761 tcgcatccat cagaccctaa tggaaagcgc ctcactgacc tatgaacagc gtctgttggc
     5821 gattcaacaa ctggggcgtg attacatggc cgccgggtta tacgaccgcg cggaagacat
     5881 gttcaatcag ctgaccgatg aaactgactt ccgcattggc gcgctgcaac agttgctaca
     5941 aatctaccag gctaccagcg agtggcagaa agcaattgat gttgccgaac gcctggtgaa
     6001 gctgggtaaa gataaacagc gcgtcgaaat tgcccatttc tactgtgagt tagccctgca
     6061 gcatatggcc agcgacgatc tcgatcgtgc catgaccttg ctaaaaaaag gggcggcggc
     6121 agataaaaac agcgcccgcg tatccatcat gatgggacgc gtgtttatgg cgaaaggaga
     6181 atacgccaaa gccgtcgaaa gtttgcaacg cgtgatatcc caggacagag aactggtcag
     6241 cgaaacgctg gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg ccgaatgggc
     6301 agaattcctg caacgcgcgg tggaagagaa caccggtgcc gatgctgaat tgatgcttgc
     6361 tgatatcatc gaagcgcgcg acggtagtga ggccgcacag gtctatatta cgcgccagct
     6421 tcagcgtcat ccgaccatgc gtgtgttcca taagttaatg gattaccact taaatgaagc
     6481 ggaagaaggg cgtgccaaag agagcctgat ggtgctgcgt gacatggttg gcgaaaaggt
     6541 acgtagtaag cctcgttatc gctgccagaa atgtggtttt accgcataca ccctctactg
     6601 gcattgtccg tcttgtcggg cctggtcaac cattaaaccg attcgcggtc ttgatggcct
     6661 gtaattttta aaaaaatccg actttagtta caacatacta gttattgatg ttccattgtg
     6721 ctccggcaac gacggagcag aaaaagcctg ccaggggaaa aatcgcaact gttaattttt
     6781 tatttccacg ggtagaatgc tcgccgttta cctgtttcgc gccacttccg gtgcccatca
     6841 tcaagaaggt ctggtcatga cgttaactgc ttcatcttct tcccgcgctg ttacgaattc
     6901 tcctgtggtt gttgcccttg attatcataa tcgtgatgac gcgctgtcct ttgtcgacaa
     6961 gatcgaccca cgcgattgtc gtctgaaggt cggcaaagag atgtttacat tgtttgggcc
     7021 acagtttgtg cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc tgaaattcca
     7081 cgatattcct aacactgcag cgcacgctgt cgctgctgca gctgacttag gcgtgtggat
     7141 ggtgaatgtt catgcctctg gtggggcgcg tatgatgacc gcagcgcgtg aggcactggt
     7201 tccgtttggc aaagatgcac cgcttttgat tgctgtgaca gtgttgacca gcatggaagc
     7261 cagcgacctg gtcgatcttg gcatgacact gtcacctgca gattatgcag aacgtctggc
     7321 ggcactgacg caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg
     7381 ctttaaacag gtattcggtc aggagttcaa actggttacg ccgggcattc gtccgcaggg
     7441 gagtgaagct ggtgaccagc gccgcattat gacgccagaa caggcgttgt cggctggtgt
     7501 tgattatatg gtgattggtc gcccggtaac gcaatcggta gatccagcgc agacgctgaa
     7561 agcgatcaac gcctctttac agcggagtgc atgatgagtg attccaacag ccgtctggtc
     7621 tactcaacgg aaaccggacg tattgatgag cccaaagcgg cccctgtacg ccctaaaggc
     7681 gacggtgtgg tgcgtattca gcgtcagacc agtggacgta aaggtaaggg cgtttgcctg
     7741 attaccggtg tcgatctcga tgatgccgaa ctgacaaaac ttgcagcgga actgaagaaa
     7801 aaatgcggct gcggcggagc agttaaagat ggagttattg aaatccaggg cgataagcgt
     7861 gatttattaa aatcactact tgaagcgaaa gggatgaagg taaaactcgc aggcggttaa
     7921 cataaaaagc cacggatata tccgtggctt tcgaatattt tactgtgcgt attattcagt
     7981 tatttcgtac tgaacagaat caagcgtatt ataattgcga gtcgataatc gacatttatt
     8041 tacgattatt taccgacctg gtgaccaata acaccaccga cagctgcacc acctaatgta
     8101 cccaacgtac tgccatcggt cagtactgca ccgcctaatg cccctgcacc cgcgccgatt
     8161 gcggtgttgc ggtcccgttt agaccagtta gaacaggcac tcagagacat tgccaaagta
     8221 attgccagaa cagccgcggt catttttttg ctcgttacaa acataatact ctctcctgaa
     8281 tttatgattc acggaagtaa gctctctata actataatag ctaagaataa gcctggtgaa
     8341 attattccgt gaaatctgct cgcgcgcagg gttatatcac gcaggtgata atgacttcct
     8401 gttatat
//
LOCUS       DAZOZD010000176         8405 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOZD010000176 DAZOZD010000000
VERSION     DAZOZD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.176"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(85..291)
                     /locus_tag="ACHUPB_003261"
     CDS             complement(85..291)
                     /locus_tag="ACHUPB_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0760000.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            604..693
                     /gene="kdpF"
                     /locus_tag="ACHUPB_003262"
     CDS             604..693
                     /gene="kdpF"
                     /locus_tag="ACHUPB_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0760001.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            693..2366
                     /gene="kdpA"
                     /locus_tag="ACHUPB_003263"
     CDS             693..2366
                     /gene="kdpA"
                     /locus_tag="ACHUPB_003263"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0760002.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2389..4437
                     /gene="kdpB"
                     /locus_tag="ACHUPB_003264"
     CDS             2389..4437
                     /gene="kdpB"
                     /locus_tag="ACHUPB_003264"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR0760003.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4446..5018
                     /gene="kdpC"
                     /locus_tag="ACHUPB_003265"
     CDS             4446..5018
                     /gene="kdpC"
                     /locus_tag="ACHUPB_003265"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0760004.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5011..7695
                     /gene="kdpD"
                     /locus_tag="ACHUPB_003266"
     CDS             5011..7695
                     /gene="kdpD"
                     /locus_tag="ACHUPB_003266"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR0760005.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7692..8369
                     /gene="kdpE"
                     /locus_tag="ACHUPB_003267"
     CDS             7692..8369
                     /gene="kdpE"
                     /locus_tag="ACHUPB_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR0760006.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1749 a         2196 c         2442 g         2018 t
ORIGIN      
        1 taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc gctggcggtt
       61 ttcaatgtat tcagaactca gcaatcagta aaaatcaccg gttgcctttt cggcctgatc
      121 catccacacc ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg
      181 ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata
      241 tgtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc
      301 ccccttttgt atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa
      361 atagtaccgc cttttgtgta attttactac tcatccgacc acttattttt gcttattgat
      421 ggtttattta cattcactct gtaattaagt tacacaaaag ttaaattaat actaaacatt
      481 agttaaatca tggcttttgc catttttata ctttttttac accccgcccg cagatttttg
      541 cgaaatcttt gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc
      601 actgtgagtg caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg
      661 gtttatgccc tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg
      721 ccacgttttt actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc
      781 tgattaatga cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac
      841 ctggcgtctc tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga
      901 acatgctggg gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta
      961 atccacagca gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg
     1021 tcacgaatac caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga
     1081 tggcaggctt aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg
     1141 ccttcatccg tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc
     1201 tgctacgcat cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta
     1261 ttcaacaagg tgcgctgcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag
     1321 cgcaacagct gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta
     1381 ctaacggcgg tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac
     1441 tgaccaactt cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct
     1501 ttggtgaagt gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga
     1561 tttttgtcat ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc
     1621 tggcgctcgg cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc
     1681 tggtcagtag cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga
     1741 tgcatgattc gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg
     1801 aagtggtgtt cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg
     1861 cggtgtttat tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg
     1921 acgtacgcga gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga
     1981 tgggcgcggc gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctggcc
     2041 cgcatggttt tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg
     2101 cctttgccgg attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt
     2161 ttgtcggtcg cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta
     2221 aaaagagcca acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc
     2281 tgttaatcgg caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg
     2341 gtccggtggc ggaatatctc tcctgatgat attgagcgag cactgaatat gagtcgtaaa
     2401 caactggcgc tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa
     2461 ttaaacccgc aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg
     2521 ctgaccacct gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt
     2581 agcgcggcaa ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg
     2641 ctggcagaag gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc
     2701 tttgcccgca agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat
     2761 caactgcgta aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt
     2821 gaagtcattg aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg
     2881 gtgatccgtg aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct
     2941 gactggctgg tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc
     3001 gcgatggtgg aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg
     3061 ctgattgctc tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg
     3121 tggggcggta atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca
     3181 accactattg gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc
     3241 gcgaatgtga ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg
     3301 ctactggata aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc
     3361 gcgcagggcg tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat
     3421 gaaacgccgg aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag
     3481 cgcgatgtgc agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc
     3541 gggatcaaca tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat
     3601 attgaggcta acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg
     3661 cgtcagggag ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg
     3721 ctgaaagata tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaaatgggc
     3781 attaaaacgg tgatgattac cggcgataac cgtctgactg ccgccgcgat agctgcggaa
     3841 gcgggtgtcg atgattttct cgccgaagca acaccggagg ccaagctggc attgatccgt
     3901 cagtatcagg cggaaggtcg tttggtagcg atgaccggcg acggcaccaa cgatgctccg
     3961 gcgctggcgc aggcagatgt cgcggtggcg atgaactccg gcacccaggc ggcgaaagag
     4021 gcgggcaata tggtggatct cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt
     4081 ggcaaacaga tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg
     4141 gcgaaatact tcgccattat tccggcggcg ttcgcggcaa catatccgca gttaaatgcg
     4201 ctgaacatca tgcgcctgca ttcgcccgac tccgccattc tcagtgcggt cattttcaac
     4261 gccttgatta tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt
     4321 accgcttctg ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg
     4381 ccgtttattg gtatcaaagt cattgattta ctgctgaccg tttgcggtct ggtgtgaggt
     4441 ttaccatgag tggattacgt ccggcattat cgacatttct ctttctgtta ttgattactg
     4501 gcggcgttta cccgctgctg accaccgcac tggggcaatg gtggtttccc tggcaggcca
     4561 acggttcgtt gatccgtgaa ggtgatacgg tgcgtggctc ggcattaatc gggcagaatt
     4621 ttaccggcaa cggctatttt catggtcgcc cgtcagcaac ggctgaaatg ccctataatc
     4681 cacaggcttc tggcgggagc aatctggcgg tcagtaaccc tgagctggat aaactaatag
     4741 ccgcacgcgt tgctgcatta cgggccgcta acccggatgc cagcgcgagc attccggttg
     4801 aactggtgac ggcatcggca agcgggctgg acaataatat caccccgcaa gcggcggcct
     4861 ggcagatccc acgcatcgcg aaagcgcgta atctcagcgt tgaacagctc acgcagctga
     4921 tcgcaaaata cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg
     4981 tcgaactcaa tctggcgctg gataagcttg atgaataacg aacccttacg tcccgacccc
     5041 gatcgtctgc tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcggt
     5101 gcctgtgcag gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg
     5161 caagggctgg atattgtggt tggcgtggta gaaacccacg ggcgaaaaga taccgccgca
     5221 atgctggaag ggctggctgt tctgccgcca aaacgcctgg cgtaccgtgg gcggcacatc
     5281 tgcgagtttg acctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa
     5341 ctggcgcaca gtaatgcgcc aggttctcgt caccctaaac gctggcagga catcgaagag
     5401 ctgctggaag caggcattga tgttttcact accgtcaacg ttcagcatct ggaaagtctg
     5461 aatgatgtgg tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt
     5521 ttcgatgccg ccgacgacgt ggttctggtg gacttgcccc cggacgatct gcgccagcgg
     5581 ctgaaagaag gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc
     5641 cgcaaaggca atctgatcgc cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt
     5701 gatgagcaaa tgcgcgcctg gcgggggcat cctagcgaag agaaagtctg gcacacgcgc
     5761 gacgcgatcc ttttatgcat cggccataac accggtagtg agaagctggt ccgcgcggcg
     5821 gcgcggctgg cgtcacggct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc
     5881 ctgcatcgct taccggagaa aaaacgtcgt gcaattctca gcgccttacg tctggcgcag
     5941 gaactgggcg cggagacggc aacactttct gatccagcgg aagagaaagc ggtagtgcgt
     6001 tatgcccgtg aacataatct cggcaagatt attctcggtc gcccggcctc gcgccgctgg
     6061 tggcgtcggg aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg
     6121 ctggtcgcgc ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct
     6181 tttaaagaca agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc
     6241 gttatcacct taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg
     6301 ctgtatctgc ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt
     6361 gccaccgtca ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc
     6421 gccgtctctg atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg
     6481 atcgggaacc ttactgctgg cgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa
     6541 cgcacacggc acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac
     6601 atcgccgcca ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg
     6661 ttgcccgatg acaacggtaa attgcagccg ttaacacatc cgcaaggaat gacgccatgg
     6721 gacgatgcca tcgcgcagtg gagttttgat aaaggcctgc ctgcgggcgc gggcaccgac
     6781 acgttacccg gtgtaccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacggg
     6841 ctggtggtgg tggagccggg gaatctgcgc cagttgatga tcccggaaca gcagcgcctg
     6901 ctggagacgt ttacgctgtt agtcgccaat gcccttgagc ggctgacgct aaccgccagc
     6961 gaagaacagg cgcggatggc aagcgaacgt gaacagatcc gcaacgccct gctggcagcg
     7021 ctttcgcatg atttacgcac gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg
     7081 ctcgatctgg caagcgaagg atcaccccac gcccgccagg ccagcgagat tcgtcagcat
     7141 gtgctgaaca ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc
     7201 ggctttaatt tgaagaaaga gtggttaacg ctggaagaag tagtcggcag cgcgctgcaa
     7261 atgctggaac cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta
     7321 atccacgttg acgggccact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg
     7381 aaatatgcgg gtgcgcaggc cgaaattggt atcgatgccc actttgaggg cgaaaatcta
     7441 caactggatg tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt
     7501 gataagtttg ctcgcgggaa taaagagtcg gcggtgccgg gtgtcgggct tgggttggca
     7561 atttgtcggg cgattgtgga tgtacacggc ggcaccatta ccgcgttcaa ccgaccggaa
     7621 ggtggtgcct gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagacttt
     7681 catgaggata tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc
     7741 tgcgcacggc gctggagggc gacgggatgc gcgtctttga ggccgaaact ctgcaacgcg
     7801 gcttgctgga agcggcaacc cgtaagccag atttgattat tctcgatctc ggcctgcccg
     7861 atggtgatgg gattgagttt atccgcgacc tgcgccagtg gagcccggtg ccggtgattg
     7921 tgctttccgc acgcagcgaa gagagcgaca agatcgccgc gctggatgcc ggagcggatg
     7981 attatctgag taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac
     8041 gccgccactc cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg
     8101 atttagccgc ccgcgtgatt caccggggtg aggaagaggt gcatctcaca ccaattgagt
     8161 tccgcctgct ggcggtgctg ctcaacaatg ccggaaaagt actcacccag cgccagctcc
     8221 ttaaccaggt gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg
     8281 gacatctgcg acaaaaactg gaacaggatc cggcccggcc tcgccacttc attactgaaa
     8341 ccggtattgg atatcggttt atgctttgaa ttgttattca ataaattcgc ttaaatagtg
     8401 attaa
//
LOCUS       DAZOZD010000177         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOZD010000177 DAZOZD010000000
VERSION     DAZOZD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.177"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUPB_003268"
     CDS             <1..188
                     /locus_tag="ACHUPB_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760007.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUPB_003269"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUPB_003269"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0760008.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUPB_003270"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUPB_003270"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0760009.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUPB_003271"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUPB_003271"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0760010.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUPB_003272"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUPB_003272"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0760011.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUPB_003273"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUPB_003273"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0760012.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUPB_003274"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUPB_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0760013.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUPB_003275"
     CDS             7142..7351
                     /locus_tag="ACHUPB_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0760014.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUPB_003276"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUPB_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0760015.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8226..>8332
                     /gene="yjbL"
                     /locus_tag="ACHUPB_003277"
     CDS             8226..>8332
                     /gene="yjbL"
                     /locus_tag="ACHUPB_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0760016.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGS"
BASE COUNT         1919 a         2014 c         2261 g         2138 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcactaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacggt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagttc agaaattatg agcgattttt
     8161 gatagtgacc agatggcaaa agaattataa ggaggacacg gtacgacaaa aaaggtagat
     8221 gaatcatgtt gaagataata cccggtgcaa ctggatactt taataaaaca ctcaattcaa
     8281 atcaattcga taatgaagat gcaatcaaag ataaattaga taatagaggt tc
//
LOCUS       DAZOZD010000178         8296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOZD010000178 DAZOZD010000000
VERSION     DAZOZD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.178"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            500..1771
                     /locus_tag="ACHUPB_003278"
     CDS             500..1771
                     /locus_tag="ACHUPB_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0760017.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="ACHUPB_003279"
     CDS             complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="ACHUPB_003279"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR0760018.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="ACHUPB_003280"
     CDS             complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="ACHUPB_003280"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0760019.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="ACHUPB_003281"
     CDS             complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="ACHUPB_003281"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0760020.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="ACHUPB_003282"
     CDS             complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="ACHUPB_003282"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0760021.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="ACHUPB_003283"
     CDS             complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="ACHUPB_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0760022.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7994..>8296)
                     /locus_tag="ACHUPB_003284"
     CDS             complement(7994..>8296)
                     /locus_tag="ACHUPB_003284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760023.1"
                     /translation="VGLIRRASVASGNGTAFIVGCGATALFDQHTTHFPANFAPHISL
                     SINGFVTETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1911 a         2250 c         2097 g         2038 t
ORIGIN      
        1 catgcccagc tctgcgagcg tcatatagcc gcctgtgttg taatgacaac gtttcgcggc
       61 tattcttgag tggtctaggt tcaagattag cccccgttat gttgtcaggt acatacctgc
      121 aacgtgcggg ggttttctct ctccagcaac caatgccacc agggataaag cccccgcaac
      181 attgcgcctc accggaaccc tttcggcttg ccgctgattt tacgattgct ttttactgtc
      241 agcacccggc aatcttcctc attgcgcgat gccttccagc ttaatttctt tatttgcaat
      301 agttgcagtt catatccggg ctgactccag actggtagtg aaatgcgcaa ttttgtagaa
      361 ttgcgcctcc tcgcgacctc cccagtgaat ctgttgcctc attttttaca tactgcgtat
      421 tcgacttctc cacctgttgc gcaagagaaa ctgggtttat tcatttttgc gaggccgact
      481 tctttctgga caggacttta tgcagcacaa cacactatcg aaacacaatc agaaattgcc
      541 gtttacacgc tacgacttcg gctgggtttt attatgcata ggcatggcga ttggtgccgg
      601 aaccgtgctg atgccagtac aaattggctt gaagggaatt tgggtattta ttaccgcagc
      661 gatcattgct tatcctgcca cctgggtagt gcaggacatt tatttaaaaa ccctttctga
      721 aagcgattcc tgtaatgact acaccgatat tatcagtcat tacctgggga agaactgggg
      781 aattttcctc ggggttatct actttttgat gattatccac gggattttta tctactctct
      841 ctccgtggtt ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg atgccgatct
      901 ttcacaatct ctactttata aagtcgctat tttcgccgta ctggtagcga ttgcgtctgg
      961 tggtgaacga ttactgttta agatttccgg gccaatggtg gtggtcaaag tagggattat
     1021 tgtcgtgttc ggttttgcga tgatcccgca ctggaatttc gccaatataa ccgccttccc
     1081 gcaagcctcc gtctttttcc gcgatgtctt gcttaccatt ccattttgct tcttttctgc
     1141 agtatttatt caggtactta acccaatgaa tattgcctat cgtaaacggg aagcggataa
     1201 agtactggca acccggctcg cgctgcgtac ccaccgaatt agttatatca cgctcatcgc
     1261 ggtgatcctg ttttttgcct tttcgtttac cttctcaatt agccacgaag aagccgtttc
     1321 tgcctttgaa caaaatatct cagcactggc gctggccgcg caggtgatcc ctgggcatat
     1381 cattcatatc acctctacgg tgcttaatat ctttgccgta ctgaccgcat tctttggcat
     1441 ttatctcggt ttccacgagg ccattaaagg cattattctc aatctgttaa gccgaattat
     1501 tgataccaag aaaattaact cacgcgtgct gactctggcg atctgcgctt ttatcgtcat
     1561 tacgttgacg atttgggttt cgtttcgtgt atcggtgctg gtgttctttc agttgggaag
     1621 cccgttatat ggtattgtgt cgtgcctcat tccgtttttc ctgatctata aagtcgcaca
     1681 actggaaaaa cttcgcggat ttaaagcctg gctgattctg ctgtacggca ttttgctatg
     1741 cttgtcgcca ctgttgaagc tgattgagta aaccggagcg catggccccg gttttgtgag
     1801 ttaacgctgc ggattttcat cctgatcaac agcaaaacaa gctaccagtt gaccgccgta
     1861 gtcttttagc tgcggctgca actgggtgca ggggccgaag cgccgacgac agcgggcgtt
     1921 gaaggcgcaa cccggcggtg gattcagtgg gcttggtagt tcaccgctga gcttgatgcg
     1981 ctcgcggcga tcgtccgggt tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg
     2041 atggcgcggg ttattgaaga tttggtcttt cgttcccttc tccacgcagc ggcccaggta
     2101 catcaccatc acttcatcag caatgtgctc caccaccgac aggtcgtggg agataaagac
     2161 ataagacagc cccaactcct gctgcaaatc catcatcaga ttcagcacct gcgcgcgcac
     2221 tgaaacatcc agcgcggaaa ccggttcatc ggcaatcacc acatccgggt cgagcatcag
     2281 accacgggcg atggcgatac gctgacgctg accgccggag aacatatgcg gatagcggtc
     2341 atagtgctcg gttttcaggc cgactttcgc catcatcgac agggcttttt cccgacgctg
     2401 ttctttgctt aagctggtgt tgatcagcag cggctcttca agaatttgcc cgactttttt
     2461 acgcggattc agcgaaccgt aagggttctg gaagacgatc tggattttct gccgacgcag
     2521 cttctgcgcc tgcggatcgt gcttaagcag atcctgcccc tggtaataca gctcgccacc
     2581 ggtgggcgtt tcaatcatcg tcagcaaccg accgagggtc gatttaccgc agccagattc
     2641 gcccactact gccagcgttt tgccacgttc aaggttaaac gaaacgccat ccagcgcttt
     2701 aaccagacgt tccggcgcga acatgccttt cttcaccgga taatgttttt tcaggtcgat
     2761 agcctgcaac agcggttgtt gcagggtggc ctcttgcgta ctcatagtgt cggcctcccg
     2821 gcatcatcaa gtgggtaatg gcatttggac tggcgcccgt cagcgagcat attcagcgcc
     2881 ggttcttcag cgcgacatct gtcagtggca taggggcagc gcgggttaag caggcagccg
     2941 ttcgggcggt cgtacttgcc gggaacgaca cctggcaacg acgccagacg ttctttgtcc
     3001 tgagcaaatt ctggcagcgc acgcagcaat gcctgagtat acgggtgacg cggcgcatgg
     3061 aagatggcgt gcgcatcgcc cgtttccacc acctggcctg catacatcac aatgatttta
     3121 tgcgcagctt ccgccaccag cgccagatca tgggtaatta acaccagcgc catgttctct
     3181 ttctgctgta gctccagcaa cagttcgatg atttgcgcct gaatggtcac gtccagcgca
     3241 gtggtcggct catcggcaat cagcagtttt ggccgacagg caatcgccat ggcgatcatc
     3301 acgcgctggc tcatgccgcc ggaaagctga tgcgggtaaa catccagacg cgatgccgga
     3361 tcgggaatac cgacctgatt cagcaggtca atcgcccgct gacggcgagt gctcttatta
     3421 ccgccctgat gcaccttaat cgcttccatg atctggaaac ccacggtgta gcacgggtta
     3481 aggctggtca tcgggtcctg gaagatcatc gccacttcgg cacccaccag gttgcggcgc
     3541 tctttttctg atatacgctg caaatcctga ccgttaaaca ccagcttttc cgccattacg
     3601 cggcccggat aatcaatcag ccccataatc gccagtgaac tgaccgactt accggagcca
     3661 gactcaccca caatcccgac cacttcgccc tgttttacgc tgtagctgat gcggtctacg
     3721 gcgcggaacg gtgcgctttc gtcgccgaaa tgcaccgata atttatctac atttaataac
     3781 gccatctcga acctcttact gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc
     3841 catcaggtta aatgccagca ccgtcagcag gatcgccaga cccgggaagg tcacgaccca
     3901 ccaggcgctt tgcgcgaact gcaacacgtc ggagagcatg gtgccccact ctggtgttgg
     3961 cggctgcgct cccatgccga ggaagccaag ggcggccata tcgagaatgg cgttagagaa
     4021 accgagcgac gcctgaacaa tcagcggcgc caggcagttc gggaagatgt taataaacat
     4081 ctgacgcatc gccccggcac ctgccacgcg agacgcggta acgtaatcgc gattcacctc
     4141 caccagcacg gcggcgcggg ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc
     4201 cagcgcggcg ttaccaatcg acgggccgaa aatcgccacc agcaccagtg ccaacagcag
     4261 acttggcagc gccagcatga tatcgaccac gcgcatgatg atgttatcga ccaggccgcc
     4321 gaagtaaccg gcgatcaggc cgagaataac gcccataatc agcgataaca ccaccaccag
     4381 acagccaacc agcagcgaca agcgcgcgcc gtacatcagc cgcgacagca catcacggcc
     4441 tacgtcatcg gtgcccagca agtgcgccat gctgccgcct tcctgccagg ctggcggggc
     4501 gagcagcgca tcgcggaact gatccgccgg gttataaggc gcaatccagt tggcaaagat
     4561 cgtgataaac agcacgatga cgatgtaaac cagcccgaca accgcgcctt tgttgcgttt
     4621 aaaatagtgc cagaactcct gtaacggggt catcggcacc ggtgcgctaa tcactttatt
     4681 ttcagtaacc tgtgacatga tgtcccctta cttcttatga cgaatacgcg ggttcaccac
     4741 gccgtacagc agatcgacca gcaggttgac gaggataatc atcgtcgcca ccagcaatac
     4801 gccgccctgc actaccggat agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag
     4861 accgggccac gagaagatgg tttcggtcag aatcgccccc gccagcaatg ttcccacctg
     4921 caggccgata acggtcacta ccggcagcat cgcgttacgc agcgcatgga cgataatcac
     4981 ccgcatgcgg gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc
     5041 cagcatcgaa gagcgcgtca tacgaacgat aaccgccagc gggatagtac ccagcacaat
     5101 agccggtaag atcatatggg caacggcgtc gataaagttg ccatcttcac cccagatggc
     5161 ggtgtcgatc agcataaaac cggttaacgg attggagtca tcgaggaaca ccatatcgct
     5221 cacgcgaccg gagacgggcg tcaggttcca gtgcaccgaa accagcatga tcagcatcat
     5281 gccccaccag aagatcggca tcgagtagcc ggtcagcgcc aggccaactg cagtgtgatc
     5341 gaaaatggaa ccgcgtttaa ccgcagccag cacaccaacc ggaataccga ctgccgtagc
     5401 aaaaatcatc gcgcagacgc caagttccag cgtggcctga aagcgcggca cgaactcttc
     5461 ccatactgga attcggcttt tcattgaaat gcctagatcg ccgtgcatta cgccccaaat
     5521 gtaatggaga tactgctgcc acatcggttt atctaagccg agttcagcca gcagctgcgc
     5581 gtgacgctca ggggagatcc cgcgttcgcc tgccatgatc atcaccggat cgcccgggat
     5641 catgtggaca aaggcaaatg tgagaagggt aataccgata aacgtgggga tgacgagtcc
     5701 caaacgtcgg agaataaact gcaacataac ccggattctc tatagtgacc cgcagcctgg
     5761 aactgcgtgt ctgtattgct cacaaatcaa ttcccgccct tatcggacag ggaatgaagt
     5821 attgctgccg gatggcgctg cacgcagagg cacatcgcca ccgggtatac agattgttga
     5881 caaagtgcgc tttgtttatg ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg
     5941 tgcaaattca atacattgca gaattttcgt aggcctgata agcggagcac atcaggcagt
     6001 tttgcctttg ccatcagtct tgtatggctt ttaattattc gatagagacg ttttcgaagt
     6061 gatgtttgcc taatggatca accacatagc ctttgacttc tttgcgtacc ggttcaaaca
     6121 cggtggagtg agcgatgatc agtgccggag tctgatcgtg catcaccacc tgcgcttgct
     6181 tgtacagttc aacgcgtttg ttatggtcgt cggtagcacg cgccggttga atcagatctt
     6241 caaacggttt gtagcaccat tttgagtagt tagaaccttg ttcagaggcg gcgcagctga
     6301 acagggtggc gaagaagtta tccgggtccc cgttatcgcc agtccagccc atcattaccg
     6361 tctgatgttc gccatctttc gcacgcttga ggtactcacc ccattcgtag gtgacgattt
     6421 tggcctgcac gcccactttc gcccagtctg cctgaatcat ctccgccatg cggcgagcgt
     6481 tcgggttata cggacgttgt accggcatcg cccacaggtc gatggagaaa cctttttcca
     6541 gacccgcttc tttcagcaag gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt
     6601 cgtcgttata gccccacatg gttggcggga tcaggttttt cgctgataca cccgcgccct
     6661 gataaaccgc tttgatgatc gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt
     6721 tcacgtcatc cagcggtttt ttctgcacgt tataggagag gtagccgacg ttcagccccg
     6781 gcatttccat caggttgatg gatttatcct gcttcatgcg agcgatgtct gccgggttcg
     6841 ggtacggcat cacctggcat tcattcttct gcaatttcgc gtaacgcacg gaagcatcag
     6901 gggtaataga gaaaaccagc gtatcgatct gcggtttggt gccccagtag ccatcaaacg
     6961 ctttgtagcg gatacgggaa tctttttggt actgctgtaa ctggaacgga ccggttccga
     7021 ttgggttgag gtccagtttt tccggtgtac cggctttcat catcgcatca gcatattctt
     7081 ttgacagaat agaggcgaag tccattgcca gatcagcgag gaacggcgct tccgggcgag
     7141 tcagtacgaa ctgaactgtg ttgtcgtcca cctttttcac ttcactgatc agctccggca
     7201 agcccatgcc ttcgaagtat tcgtagctgc cgccagagac tttatggtac gggttttgcg
     7261 cgtttttctg acggtcgaac gagaacacca cgtcatcggc gttcagttca cgcgtcggtt
     7321 tgaattcttt attgtcgtgc cacttcacac ctttacgcag atggaaggta taggttttac
     7381 cgtcttcgct gacttcccac ttttcagcga ggcccgggat cacttcagtg gtgccgattt
     7441 taaattcaac caggcggtta taaagcggta cggaagaggc gtcataggtg gtgccggagg
     7501 taaacagctg cgggttaaac ccttccggag atccttctga gcaataaacc agagttttag
     7561 cctgaacact tgctgcgacg gtcatagcca ccaggctgag accaagcttc agcatccctg
     7621 actttttcaa ggaaatacgc attattctgc tccaattgtg atgtttgttg ttttaaccct
     7681 ttgcagtggg ttgtcgctgc ctgacctttt ttgttttttg cccggtcggg tcaacgttat
     7741 gaggtgggga tgccgtacta attaacatca gtgtaacagt gcggatcctc cataaaatgc
     7801 ccctcgtgac ctacaatctg tcaacagaat gtgaaaacgt caatacagcc aaccgggatt
     7861 tacaccaacg gtgagaatcc acaaacaaag attaaaaaaa cttcaaacag ctatttgcag
     7921 cagagaaatt tgtgctactc caacataacc agaacaatca gcttaatatt tagcaacatt
     7981 tggtgattaa gttttatgca tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc
     8041 gattaatacc atgaacgcta aaacgcacag cggaaaatgc cagcgccagc cataagtaac
     8101 gcaaaaaaag atttaagcaa atataaaaaa agacaatggt ttctgtcaca aatccgttaa
     8161 tggatagtga gatatggggc gcaaagttgg caggaaaatg tgtggtgtgt tggtcgaata
     8221 aggcggtcgc gccgcatccg acaataaatg cggtgccgtt gcctgatgcg acgctggcgc
     8281 gtcttatcag gcctac
//
LOCUS       DAZOZD010000179         8187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOZD010000179 DAZOZD010000000
VERSION     DAZOZD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.179"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(188..1078)
                     /gene="malG"
                     /locus_tag="ACHUPB_003285"
     CDS             complement(188..1078)
                     /gene="malG"
                     /locus_tag="ACHUPB_003285"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0760024.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1093..2637)
                     /gene="malF"
                     /locus_tag="ACHUPB_003286"
     CDS             complement(1093..2637)
                     /gene="malF"
                     /locus_tag="ACHUPB_003286"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0760025.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2791..3981)
                     /gene="malE"
                     /locus_tag="ACHUPB_003287"
     CDS             complement(2791..3981)
                     /gene="malE"
                     /locus_tag="ACHUPB_003287"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0760026.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4346..5461
                     /gene="malK"
                     /locus_tag="ACHUPB_003288"
     CDS             4346..5461
                     /gene="malK"
                     /locus_tag="ACHUPB_003288"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0760027.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5533..6873
                     /gene="lamB"
                     /locus_tag="ACHUPB_003289"
     CDS             5533..6873
                     /gene="lamB"
                     /locus_tag="ACHUPB_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0760028.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7025..7945
                     /gene="malM"
                     /locus_tag="ACHUPB_003290"
     CDS             7025..7945
                     /gene="malM"
                     /locus_tag="ACHUPB_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0760029.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         2016 a         2133 c         2123 g         1915 t
ORIGIN      
        1 aattatcgta agtgggaaaa caaataacgt aaaaataata agctctatga tggaaatatt
       61 aaccggcgaa cgattcagat tgcagacgaa agaaaaaaag gcgctccgtg gagcgccgaa
      121 taacagtcac aagttgggat aacgtaagtt gagggtgcag cggcataaca ttggcagaac
      181 aacatcttta acctttcaca ccacctgccg tcaggccgtt gaccagccag cgttgagcca
      241 gcaagaagac gatggtgatc ggtaatgcag acatcacggc agcggcggca aagtcacccc
      301 acaggtagtt ttgcgggttg aggtattgct gcatccccac ggccagggtg tagctgttta
      361 cgtcacgcag taacagcgac gcgaccggaa cttcagtaat ggcagcgata aacgacagga
      421 tgaataccac cgccagaatc ggtactgaca gcggcaacag gacaaggcgg aaggcctgcc
      481 acggtgtcgc accatccagc gcagccgctt cttccagcga actatcgatg gtttcgaaat
      541 agcctttgat ggtccagaca tgcagcgcaa tcccacccag atacgcgaaa attacgccgc
      601 cgtgagtatt caggccaatg aatggaatgt actcacccag acgatcaaac aacgcataca
      661 acgcgaccag tgaaagtact gccgggaaca tctggaaaat cagcattcct ttcagcagcg
      721 tcgctttgcc tggaaagcgc atacgggcga aagcgtaggc gcaggtggta gagagcgcga
      781 caatgccaat cgcggaaatc ccggcgacct ttaccgagtt ccatagccac agcagtaccg
      841 ggaatggcgg tggcgtaatg cgaccatcag cctgttccac gctaaaacct aaagccagtt
      901 tccagtgatc ccaggagatt tgctccggga tcaggctgcc gatcgcaaag ttcccctggc
      961 gcagtgagat agcgacgacc atcagcagcg ggaacataat cgccgcgata aaaagtagca
     1021 gtagcaggtg agtaataaac aaacgtgctt tttgcgattt cggttggacc attgccattt
     1081 ttgttatctc ccttaatcaa acttcattcg cgtggctttc aggttcacta tcgccagcgc
     1141 acccaccagc aggaagagca gcgtggcaat tgctgcagcc agaccgaagt cctgaccccc
     1201 gccgccttca aaagcgatac ggtaggtgta gttaacaagc aggtcggtat aaccggctgg
     1261 cgtggtcgtg ccaagacgat ccgggccgcc gttggttaac agttgaatca gcacgaagtt
     1321 gttaaagtta aaggcgaagc tggcgatcat cagcggcgtc agcggtttaa tcagcagcgg
     1381 cagcgtaatc ttaaagaagt tctggaacgg atctgcgcca tccattgctg aggcttcata
     1441 caaatcgtcc ggaatcgctt tcagcaagcc catgcagagg atcatcatgt acggataacc
     1501 cagccaggta ttgacgataa ttagcatcgt gcgggcggtg gtcggatcgc tgaaccaggc
     1561 gggcttcacg ccaaacagcg cgctcaacat catgttgatt tcaccgaagc tctggttaaa
     1621 caaccctttg aaaatcaaga ttgaaatgaa tgatggcacc gcgtagggca gaatcagcag
     1681 aacgcgatag accgctttgc cgcgcaatgc ttcccactgc accagacatg ccagaaccat
     1741 gccgaccgcc accgttaaaa agacagtgat cagcgagaac accacggtcc agacgaaaat
     1801 ggcgaggaac ggtttctgaa tgccttcgtc ggtgaagacg cgggtaaagt ttttccagcc
     1861 agtagtcacg gtgtaaccgg ggcttagctt ttcatcaccc cagttgccgt cggcggtaat
     1921 ggactggtaa aagccaattt ggttattcgg acgatatttc acgccgctct gattattcgt
     1981 caacgtgccg tcaccgtcga gtgtgtagag cggctgcgtg ccagaaaact ggcgcaggga
     2041 gctcatcatc actttgttgc catccggcag aatggcggta atgtcactca gcgcctgacg
     2101 attctgggta atcacgcgta gattcgcgcg ttcgccttcg ggctgggcgg tcgtttcttt
     2161 cagttgcagt ttttgttcgc cgccaaattt aaacgcgtcg gagaggtaat ttttgccgat
     2221 ttcgccgtca ctgagcgcca gttgccactc atcgcccgcc gggtaaagac caaagttata
     2281 ggttttgcct gcttgccagg agcgatctaa caacacttcc tgcgcacatt caaaagtcag
     2341 ctggttagtg ctgctgtagt tggtgaaggc aatggcgatg gtgcagacca gagggaagag
     2401 gacgaataat cccattccag ccattcccgg gtaaacatag cgccaggcgt aggctttacg
     2461 attggcgaaa atatacagcc ccgctgaact caatatcagc gtggtaatgg cgaacaggta
     2521 ttccccttgt gcgtacatta aaacaacaag gtaacccacc agcaggccga gcagacctag
     2581 cactgaccat ttcagcgcgt cgctttgcca ccaatgtttc tttttaatga catccatggg
     2641 gttcttcctc attccaggac ggataaggct ttcacgcctt atccgacaac aactgcctga
     2701 tgcgacactg atgcgtctta tcaggcctac atgcgtttca gttttgtagg ccggacaagg
     2761 cgttcacgcc gcatccggca tttcacagca ttacttggtg atacgagtct gcgcgtcttt
     2821 cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag tacgcacggc
     2881 ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct gggcgttttc
     2941 catggtggcg gcaatacgtg gatctttcgc caactcttcc tcgtaagact tcagcgccac
     3001 tgcacccagc ggtttgtctt tattgaccgc ttccagacct tcgtcagtca gcagatagtt
     3061 ttcgaggaac tctttcgcca gctctttgtt cggactggcg gcgttaatac cggcgctcag
     3121 cacgccaacg aacggtttag atggttgacc cttgaaggtc ggcagtaccg ttacaccata
     3181 attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg tcatcgctgt
     3241 ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt
     3301 gtttttaata aggtcaacca ggaaggtcag acccgctttc gcgccagcgt tatccacggc
     3361 cacgtcttta atatcgtact tgccgttttc atacttgaac gcataacccc cgtcagcagc
     3421 aatcagcggc caggtgaagt acggttcttg caggttgaac atcagcgcgc tcttaccttt
     3481 cgctttcagt tctttatcca gcgccgggat ctcttcccag gtttttggtg ggttcggcag
     3541 cagatctttg ttataaatca gcgataacgc ttcaacagcg atcgggtaag caatcagctt
     3601 gccgttgtaa cgtacggcat cccaggtaaa cggatacagc ttgtcctgga acgctttgtc
     3661 cggggtgatt tcagccaaca ggccagattg agcgtagcca ccaaaacggt cgtgcgccca
     3721 gaagataatg tcagggccat cgccagttgc cgcaacctga gggaatttct cttccagttt
     3781 atccggatgc tcaacggtga ctttaattcc ggtatctttc tcgaatttct taccgacttc
     3841 agcgagaccg ttatagcctt tatcgccgtt aatccagatt accagtttac cttcttcgat
     3901 tttggcgaga gccgaggcgg aaaacatcat cgtcgttaat gcggataatg cgaggatgcg
     3961 tgcacctgtt tttattttca taatctatgg tccttgttgg tgaagtgctc gtgaaaacac
     4021 ctaaacggac tctagtttct ttatacggca acctctttcc atcctccttg cccctacgcc
     4081 ccaccgttgc tttgtgtgat ctctgttaca gaattggcgg taatgtggag atgcgcacat
     4141 aaaatcgcca cgatttttgc aagcaacatc acgaaattcc ttacatgacc tcggtttagt
     4201 tcacagaagc cgtgttctca tcctcccgcc tcctccccca taaaaaagcc agggggtgga
     4261 ggatttaagc catctcctga tgacgcatag tcagcccatc atgaatgttg ctgtcgatga
     4321 caggttgtta caaagggaga agggcatggc gagcgtacag ctgcaaaatg taacgaaagc
     4381 ctggggcgag gtcgtggtat cgaaagatat caatctcgat atccatgaag gtgaattcgt
     4441 ggtgtttgtc ggaccgtctg gctgcggtaa atcgacttta ctgcgcatga ttgccgggct
     4501 tgagacgatc accagcggcg acctgttcat cggtgagaaa cggatgaatg acactccgcc
     4561 agcagaacgc ggcgttggta tggtgtttca gtcttacgcg ctctatcccc acctgtcagt
     4621 agcagaaaac atgtcatttg gcctgaaact ggctggcgca aaaaaagagg tgattaacca
     4681 acgcgtcaac caggtggcgg aagtgctgca actggcgcat ttgctggatc gcaaaccgaa
     4741 agcgctctcc ggtggtcagc gtcagcgtgt ggcgattggc cgtacgctgg tggccgagcc
     4801 aagcgtattt ttgctcgatg aaccgctctc caacctcgat gctgcactgc gtgtgcaaat
     4861 gcgtatcgaa atctcccgtc tgcataaacg cctgggccgc acaatgattt acgtcaccca
     4921 cgatcaggtc gaagcgatga cgctggccga caaaatcgtg gtgctggacg ccggtcgcgt
     4981 ggcgcaggtt gggaaaccgc tggagctgta ccactatccg gcagaccgtt ttgtcgccgg
     5041 atttatcggt tcgccaaaga tgaacttcct gccggtaaaa gtgaccgcca ccgcaatcga
     5101 tcaagtgcag gtggagctgc cgatgccaaa tcgtcagcaa gtctggctgc cagttgaaag
     5161 ccgtgatgtc caggttggag ccaatatgtc gctgggtatt cgcccggaac atctactgcc
     5221 gagtgatatc gctgacgtca tccttgaggg tgaagttcag gtcgtcgagc aactcggcaa
     5281 cgaaacccaa atccatatcc agatcccttc cattcgtcaa aacctggtgt accgccagaa
     5341 cgacgtggtg ttggtagaag aaggtgccac attcgctatc ggcctgccgc cagagcgttg
     5401 ccatctgttc cgtgaggatg gcactgcatg tcgtcgactg cataaggagc cgggcgttta
     5461 agcaccccac aaaacacaca aagcctgtca caggtgatgt gaaaaaagaa aagcaatgac
     5521 tcaggagata gaatgatgat tactctgcgc aaacttcctc tggcggttgc cgtcgcagcg
     5581 ggcgtaatgt ctgctcaggc aatggctgtt gatttccacg gctatgcacg ttccggtatt
     5641 ggctggacag gtagcggcgg tgaacaacag tgtttccaga ctaccggtgc tcaaagtaaa
     5701 taccgtcttg gcaacgaatg tgaaacttat gctgaattaa aattgggtca ggaagtgtgg
     5761 aaagagggcg ataagagctt ctatttcgac actaacgtgg cctattccgt cgcgcaacag
     5821 aatgactggg aagctaccga tccggccttc cgtgaagcaa acgtgcaggg taaaaacctg
     5881 atcgaatggc tgccaggttc caccatctgg gcaggtaagc gcttctacca acgtcatgac
     5941 gttcatatga tcgacttcta ctactgggat atttctggtc ctggtgccgg tctggaaaac
     6001 atcgatgttg gcttcggtaa actctctctg gcagcaaccc gctcctctga agctggtggt
     6061 tcttcctctt ttgccagcaa caatatttat gactatacca acgaaaccgc gaacgacgtt
     6121 ttcgatgtgc gtttagcgca gatggaaatc aacccgggcg gcacattaga actgggtgtc
     6181 gactacggtc gtgccaacct gcgtgataac tatcgtctgg ttgatggcgc atcgaaagac
     6241 ggctggttat tcactgctga acatactcag agtgtcctga agggctttaa caagtttgtt
     6301 gttcagtacg ctactgactc gatgacctcg cagggtaaag gtctgtcgca gggttctggc
     6361 gtcgcgtttg ataacgaaaa atttgcctac aatatcaaca acaacggtca catgctgcgt
     6421 atcctcgacc acggtgcgat ctccatgggc gacaactggg acatgatgta cgtgggtatg
     6481 taccaggata tcaactggga taacgacaac ggcaccaagt ggtggaccgt cggtattcgc
     6541 ccgatgtaca agtggacgcc aatcatgagc accgtgatgg aaatcggcta cgacaacgtc
     6601 gaatcccagc gcaccggcga caagaacaat cagtacaaaa ttaccctcgc acaacaatgg
     6661 caggctggcg acagcatctg gtcacgcccg gctattcgtg tcttcgcaac ctacgccaag
     6721 tgggatgaga aatggggtta cgactacaac ggcgatagca aggttaaccc gaactacggc
     6781 aaagccgttc ctgctgattt caacggcggc agcttcggtc gtggcgacag cgacgagtgg
     6841 accttcggtg cccagatgga aatctggtgg taatagcaaa acctgggccg gataaggcgt
     6901 ttacgccgca tccggcataa aaacaggttg tcattatctg aaaggggcga aagcccctct
     6961 gattatcggg tttagcgcgc tattgcctgg ctaccgctga gctccagatt ttgaggtgaa
     7021 aacaatgaaa atgaataaaa gtctcatcgc cctctgttta tcagcagggt tactggcaag
     7081 cgcgcctgga attagccttg ccgatgttag ctacgtaccg caaaacacca gcgacgcgcc
     7141 agccattcca tctgccgcgc tgcaacaact cacctggaca ccggtcgatc aatctaaaac
     7201 ccagaccacc caactggcga ccggcggcca acaactgaac gttcccggca tcagtggtcc
     7261 ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg accctgacgc tgaccagcga
     7321 agtgaacaaa caaaccagcg tttttgcgcc gaacgtgctg attcttgatc agaacatgac
     7381 cccatcagcc ttcttcccca gcagttattt cacctaccag gaaccaggcg tgatgagtgc
     7441 agatcggctg gaaggcgtta tgcgcctgac accggcgttg gggcagcaaa aactttatgt
     7501 tctggtcttt accacggaaa aagatctcca gcagacgacc caactgctcg acccggctaa
     7561 agcctatgcc aaaggcgtcg gtaactcgat cccggatatt cccgatccgg ttgctcgtca
     7621 taccagcgat ggcttactga aactgaaagt caaaacgaac tccagctcca gcgtgttggt
     7681 aggaccacta tttggttctt ccgctccagc tccggttacg gtaggcaaca cggcggcacc
     7741 agctgtggct gcacctgctc cggtgccggt gaagaaaagc gagccgatgc tcaacgacac
     7801 ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc
     7861 gttaaaactg cttgatgaag ctgaacgcct gggatcgaca tctgcccgtt ccacctttat
     7921 cagcagtgta aaaggcaagg ggtaattacg ccccacagtg ctgattttgc aacaactggt
     7981 gcgtctcctg gcgcaccttt ttttatgctt ccttcctggg atatgagcga ttttttatag
     8041 tacttcactt cttcttcact aagaatgtcc attatctcaa tgccttatca gagattcttt
     8101 tcctttcgcc ggtagtgtct ggacgctcag gctacttttc caggttattt tatttctgta
     8161 ttacagaggt tttatgatag gtcatat
//
LOCUS       DAZOZD010000180         8183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOZD010000180 DAZOZD010000000
VERSION     DAZOZD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.180"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(351..1547)
                     /gene="hemY"
                     /locus_tag="ACHUPB_003291"
     CDS             complement(351..1547)
                     /gene="hemY"
                     /locus_tag="ACHUPB_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0760030.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1550..2767)
                     /gene="hemX"
                     /locus_tag="ACHUPB_003292"
     CDS             complement(1550..2767)
                     /gene="hemX"
                     /locus_tag="ACHUPB_003292"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0760031.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            complement(2789..3529)
                     /gene="hemD"
                     /locus_tag="ACHUPB_003293"
     CDS             complement(2789..3529)
                     /gene="hemD"
                     /locus_tag="ACHUPB_003293"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0760032.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3526..4467)
                     /gene="hemC"
                     /locus_tag="ACHUPB_003294"
     CDS             complement(3526..4467)
                     /gene="hemC"
                     /locus_tag="ACHUPB_003294"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0760033.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4854..7400
                     /gene="cyaA"
                     /locus_tag="ACHUPB_003295"
     CDS             4854..7400
                     /gene="cyaA"
                     /locus_tag="ACHUPB_003295"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0760034.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7440..7760)
                     /gene="cyaY"
                     /locus_tag="ACHUPB_003296"
     CDS             complement(7440..7760)
                     /gene="cyaY"
                     /locus_tag="ACHUPB_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0760035.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7922..8064)
                     /locus_tag="ACHUPB_003297"
                     /pseudo
     CDS             complement(7922..8064)
                     /locus_tag="ACHUPB_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1819 a         2167 c         2131 g         2066 t
ORIGIN      
        1 ttaaatattc actcaggaag caaagaggat tacagaatta tctcataaca agtgttaagg
       61 gatgttattt cccgattctc tgtggcataa taaacgggta gatgctcatt ccatctctta
      121 tgttcgcctt agtgcctcat aaactccgga atgacgcaga gccgtttacg gtgcttatcg
      181 tccactgaca gatgtcgctt atgcctcatc agacaccatg gacacaacgt tgagtgaagc
      241 acccactgtt gtcatacaga cctgttttaa cgcctgctcc gtaataagag caggcgtttt
      301 tttatgcatc agaaaggtcc cggaggtgct tgcctccggg tgagaaggaa ctactgtggt
      361 gggttgttct gcaacgttaa catcaaaccg tcgcgacgca tcgctgcagc ttcttccggc
      421 ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat cgtaggcgtc cggacgttgt
      481 ttcagcgctg cgcggaaggc gagcgatgct tcctgccatt ctccgtgctt catcagtgac
      541 tggcccagtg tgctccacaa cagcgggcga tcgccgacgt ttttgatctg ctggcgcagc
      601 actttttcca gctgttccgg attgttggtt ttcagtcgag gaatcggcag cagtaggcga
      661 tcgtcgtact ggcgtttcag gccatcgatg ataatttgct gggcagtatc atgatcgtca
      721 cattcaataa gatgttccgc cattgccacc tgcaacgcta cctgatgacg cgttttccgg
      781 ctttggtttt tccaccagtt acgcaaacct tcactaccgt tatcggcacg cgcctgatcc
      841 atcaggccaa tccatgcctg ttgttccagc attgcacgat gttcttcatc accaacatgg
      901 gctttcgcca ttgatgggat gatatccagc agagaactcc atgcacctgt gcggatatac
      961 gcctgttccg ccagacgtaa tacttccgga tggcgtggcg taacttccag cagcttatcc
     1021 acgccgtggc gtgcagcatg gttttcatta cgggccagtt gcagacgtac acgggtgatt
     1081 tctaccggaa tggtgtcatt gccggccagc tctgctgcgc gttccagatg ttggttggcg
     1141 cgtgcttcat caccacgttg ttgcgcggct tcggcagcca gtagatagtt caccaccggt
     1201 tgttccgcgt gatcggcatt tttcgccatc agcttttcaa cttgctgata atcgccttcc
     1261 gccagtttca gcagcgcctg ttcggtctgc ttacgggcac ggcgacgctt acgtccgaca
     1321 aaccacccac gggtgtgtgc gcctgtgcgg aagatccgcc gcagtagcca ctcaatggca
     1381 aacagcacta ccatcgccag aatcaaaata atcgccaggc ccgtgacgct ggtttcgata
     1441 ttgtagttgt cagtctggat cagcacgtaa ccctgatgac cggcaatcat cgggccaacc
     1501 actatccccg caatcagcaa aacaaagagt aataacactt ttagcatggt tattctcctt
     1561 gcggcgcggc tgccggagta tcaccttgcg gtgcaggtgc aggtgcaggt gcaggtgcag
     1621 gtgcaggtgc aggtgcaggt ttagcttccg ttgtccccgc tgccggttgt gccagcaggt
     1681 tacgcacgcg ggtctgcatc agtttttcca gcatcgcctg gctttgcagg gtttccggaa
     1741 gatccatcga gatattttgc tggcttaact ggtccacctc gtcgaggaac gctttggtgg
     1801 tggcatcatc agtatcgtag taagcacgta cccaggtgga gacgttctcc agcgcctggc
     1861 gataagtctc ttcctgatga cgcggtacag cttgtgctgc gaccagcagg cgagagcgaa
     1921 tattttcgcg cagatagata tcctgatttg gcgctaacag cggtacggcg gtgtcatcac
     1981 gacggcgaat cgtaatgaag ttgtccataa agttctgcca gcttttttgc agattgatac
     2041 gccattcgct gatggaactg gaaagctctt caccgtctga atccatcggc gaaccatcgc
     2101 tgtcattatc ggccagacgc aggttatcta cctgatttga aagctgatta agcttaagga
     2161 tgatgccgtc ataatccacc tgcgatactg cagaaaggct ggcgatatca tcggtaattg
     2221 cccgacgaac ggtaatcaga ctcgggtcat tcatatccgc caggctggcg tctgcacttt
     2281 tcagcaacgc tgcagcggtc gtgacgtcct gatcgctcca cagcttccgt ccggcgagtt
     2341 tcaccagaaa atcggcctgg gccagcagcc aggttttagc atcgctgccg gaaatggtgg
     2401 cgaccttttg ctggacttca tccagctgtt ttgccagcgt ttcttgctga cgattcgcct
     2461 gttcaagctg cgtagcctgt tgcttaataa tgccttccag ttcggctttt tggctctcct
     2521 gggctttttg caatgccgtc agttggttag ccagggcatc gctggtggcg gtctgattga
     2581 cggcctgttg tttaccccag ccatacaaac cgacgcccgc cgccagagca atagcgatag
     2641 ccgccgcgct gagaatcaat gcggtattgt tcttactctt tttttctgtt gcgacaggtt
     2701 gtgacgtggt gtccacggcc tccctagtct cttcaaccac ggcggaggtt ttttcttgtt
     2761 ccgtcattat ggcttcctgt tatgagagtt attgtaatgc ccgtaaaagc gcatcgttgg
     2821 cagcgttatc ggcgacctta atgtcttgcc agcccagttc ccgggcgagt ttcgccaaac
     2881 gctcactgac gaccaatagt cgacagtgta gtaaccagtg ctcacgatac cattgtggga
     2941 tcagcgacca gagttgctgc aacatttcac cgctggtaac aacgaccgtc gtcacctcgc
     3001 gggattgcca gcgcatcgct tcttctgcac catcgtaatg gattgcgcat cgttgataac
     3061 attcacaaaa agtgacctca gcaccgcgcg ccgtcagggt atccccaatt agctcacgac
     3121 cgccattgcc acgtaatatc agcgcacgtt tgcccgcaat attttgtaat tcaggtaatt
     3181 gtagcaagac ttcgctgatt tcccgatcct gcgggtagag aatcttctgt ccgcttacgg
     3241 tatgtagtgc cagtgcggtg gtgcgtccaa tggcgaaata attaggtagt cggggccatt
     3301 tacgatcttg ctgatgtagc tgtgattggg caaaagcaac cgcgtgttgc gagagggcaa
     3361 acaacagatc gctctccccc agcgctgcca gttgatcagc aagttgcggt aattgttgac
     3421 ccggagaaaa ctcaatcagc ggaaaatgcc aggccacctg ccccagtgtg cgcagacggc
     3481 tcactaactc ttctccagcg ggagacgggc gggtgacaag gatactcatg ccggggcgtc
     3541 tccgttatag acttcagcga ggatctcgcg cgcgccgtta ttcagtagct cttctgccag
     3601 cgaaatcccc atttgtttgg cattttgcgg cgcaccgcgg cgttcaccgc gaataatctg
     3661 cgaaccgtcc ggcgcgccga ccagcgcacg cagccagatt tcgccatcaa taagctcggc
     3721 gtagctacca attggcacct gacatccgcc ttcgagacgg gtattcatgg cgcgttctgc
     3781 ggtaacgcgc agtgcagttt cgtggtgatt cagcgcggca agcagctcgc gagtgcgcgt
     3841 atcatcaagg cggcattcaa taccgactgc accttgtcct accgccggaa gagaaatctc
     3901 gggtggtaac gcggcgcgaa tgcgggactc cagacctaaa cgttttagtc cggctacggc
     3961 aagaatgatg gcatcgtatt cgccgttatc tagtttgctc aggcgagtgc cgacgttgcc
     4021 gcgcagggag cggataatca gatccgggcg gcgttcagcc agttggcact ggcgacgtaa
     4081 actggacgtc ccgacgatac tgcctgctgg taacgcatcc agactgtcat agttattgga
     4141 cacaaaggca tcgcgaggat cttcacgctc acaaatagtg accagtccca gaccttgcgg
     4201 gaattcaacc ggcacatctt tcattgaatg tacggcgata tcggcgcgat tttcgaggag
     4261 cgcgacttcc agctctttta caaataagcc ttttccgcct actttcgcca gcggcgtatc
     4321 aagaatcaca tcgccgcgcg tcaccatcgg taccagttca acgaccaggc ccggatggct
     4381 cgccatcaac ttgtctttga catagtgtgc ctgccagagt gcaagtgggc tttggcgtgt
     4441 ggcaattctt aaaacattgt ctaacatgct tgttaccgtc attatcatcc gtggtccatc
     4501 ctaacatcct tgccagagtg atgtcagtgt tgtggtgaaa cgtagacgcc tgcgcaaacc
     4561 gtaaaatgag gtctggcagt ggatcctgac agacgtttca cgctgtcgta ataaggaatt
     4621 tacagagaat aaacggtgct acacttgtat gtagcgcatc tttctttacg gtcaatcagc
     4681 aaggtgttaa attgatcacg ttttagacca ttttttcgtc gtgaaactaa aaaaaccagg
     4741 cgcgaaaagt ggtaacggtt acctttgaca tacgaaatat cccgaatgcc gcgtgttacc
     4801 gttgatgttg gcggaatcac agtcatgacg ggtagcaaat caggcgatac gtcttgtacc
     4861 tctatattga gactctgaaa cagagactgg atgccataaa tcaattgcgt gtggatcgcg
     4921 cgcttgctgc tatggggcct gcattccaac aggtctacag tctactgccg acattgttgc
     4981 actatcacca tccgctaatg ccgggttacc ttgatggtaa cgttcccaaa ggcatttgcc
     5041 tttacacgcc tgatgaaact caacgccact acctgaacga gcttgaactg tatcgtggaa
     5101 tgtcagtaca ggatccaccg aaaggtgagc ttccaattac tggtgtatac accatgggca
     5161 gcacctcgtc cgtagggcaa agttgttcct ctgacctgga tatctgggtc tgtcatcaat
     5221 cctggctcga tagcgaagag cgccagttac tacaacgtaa atgcagcctg ctggaaagct
     5281 gggccgcctc gctgggtgtg gaagtcagct tcttcctgat tgatgaaaac cgctttcgtc
     5341 ataatgaaag cggcagcctg gggggcgaag attgtggctc cacccagcat atactgctgc
     5401 ttgacgaatt ttatcgtacc gccgtgcgtc tcgccggtaa gcgtattctg tggaatatgg
     5461 tgccgtgcga cgaagaagag cattacgacg actatgtgat gacgctttac gcgcagggcg
     5521 tgctgacgcc aaatgaatgg ctggatctcg gtggcttaag ctcgctttct gctgaagagt
     5581 actttggtgc cagcctttgg cagctctaca agagtatcga ttccccatac aaagcggtgc
     5641 tgaaaacact gctgctggaa gcctattcct gggaataccc gaacccacgt ctgctggcga
     5701 aagatatcaa acagcgtttg cacgacggcg agattgtatc gtttggcctc gatccatact
     5761 gcatgatgct ggagcgtgtt actgaatacc tgacggcgat tgaagatttc acccgtctgg
     5821 atttagtacg tcgctgcttc tatttaaaag tgtgcgaaaa actcagccgt gaacgcgcct
     5881 gtgtaggctg gcgtcgcgca gtgttgagcc agttagtgag cgagtggggt tgggacgaag
     5941 ctcgtctggc aatgctcgat aaccgtgcta actggaagat tgatcaggtg cgtgaggcgc
     6001 acaacgagtt gctcgacgcg atgatgcaga gctaccgtaa tctgatccgc tttgcgcgtc
     6061 gcaataacct tagcgtctcc gccagtccgc aggatatcgg cgtgctgacg cgtaagctgt
     6121 atgccgcgtt tgaagcatta ccaggtaaag tgacgctggt aaacccgcag atttcacccg
     6181 atctctcgga accgaatctg acctttattt atgtgccgcc gggccgggct aaccgttcag
     6241 gttggtatct gtataaccgc gcgccaaata ttgagtcgat catcagccat cagccgctgg
     6301 aatataaccg ttacctgaat aaactggtgg cgtgggcatg gtttaacggt ctgctgacct
     6361 cgcgcacccg tttgtatatt aaaggtaacg gcattgtcga tttgcctaag ttgcaggaga
     6421 tggtcgccga cgtgtcgcac catttcccgc tgcgcttacc tgcaccgaca ccgaaggcgc
     6481 tctacagccc gtgtgagatc cgccatctgg cgattatcgt taacctggaa tatgacccga
     6541 cagcggcgtt ccgcaatcag gtggtgcatt ttgatttccg taagctggat gtcttcagct
     6601 ttggcgagaa tcaaaattgc ctggtaggta gcgttgacct gctgtaccgc aactcgtgga
     6661 acgaagtgcg tacgctgcac ttcaacggcg agcaatcgat gatcgaagcc ctgaaaacta
     6721 ttctcggcaa aatgcatcag gacgccgcac cgccagatag cgtggaagtc ttctgttata
     6781 gccagcatct gcgcgggtta atccgtactc gcgtgcagca actggtttct gagtgtattg
     6841 aactgcgcct ttccagcacc cgccaggaaa ccgggcgttt caaggcgctg cgcgtttctg
     6901 gtcaaacctg gggcttgttc ttcgaacgcc tgaatgtatc ggtacagaag ctggagaacg
     6961 ccatcgagtt ttatggcgct atttcgcata acaaactgca cggcctgtca gtgcaggttg
     7021 aaaccaatca cgtcaaatta ccggcggtgg tggacggctt tgctagtgaa gggatcatcc
     7081 agttcttctt cgaagaaacg caagacgaga atggctttaa tatctacatt cttgacgaaa
     7141 gcaaccgggt tgaggtgtat caccactgcg aaggcagcaa agaggaactg gtgcgtgacg
     7201 tcagtcgctt ctactcgtca tcgcacgacc gcttcaccta cggctcaagc ttcatcaact
     7261 tcaacctgcc gcagttctat cagattgtga aggttgatgg tcgtgaacag gtgattccgt
     7321 tccgcacaaa atctatcggt aacatgccgc ctgccaatca ggatcacgat acgccgctat
     7381 tacagcaata tttttcgtga tgaacgtgcc ggaaagcgag gcttatccgg catgcaatct
     7441 tagcggaaac tgactgtttc acccgcctgc tgcgtcgccg cctgttccag caaatcccag
     7501 aaggtttcgc cgctgcgatc gcaaatccac tcatcgcctt tcagatcaaa atggtagccg
     7561 ccttgtttgg ttgccagcca tacctggtgc agcggttcct ggcggttgat aatgattttg
     7621 ctgccgtttt caaaggtaat ggtcagcacg ccgccgttga tttcgcagtc gatatcgctg
     7681 tcgccatccc agtcgtccag gcgttcttca atggtcagcc agagttgatc agccaggcga
     7741 tgaaattcac tgtcgttcat tgttgtatcc tgtttttaag tgatggcggc agtatagcgg
     7801 catggggtca gggcttcaaa gtttgcacct ctgcggctgc gttccggcac gattcatccg
     7861 tcaccggaat aatgatgtct ctgtgtagcg aaagatttgt ctcttcatta gggcgcagtt
     7921 acaccacgcc tttccctgtt tctggtaaac attatgatca ggtttaccga gcgagcatcc
     7981 tcacgctgac ggaactaaaa aagacaacaa acaaaccaca ttgcgatagt gcatagagcc
     8041 atcctggcgc gaggtgccga tcacgaaact accagcaaaa cataaatccc cacgagtaag
     8101 cgttatactc gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc
     8161 ttttacgtct tctcctgcga tga
//
LOCUS       DAZOZD010000181         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOZD010000181 DAZOZD010000000
VERSION     DAZOZD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.181"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUPB_003298"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUPB_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR0760036.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUPB_003299"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUPB_003299"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0760037.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUPB_003300"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUPB_003300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0760038.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUPB_003301"
     CDS             6679..>8182
                     /locus_tag="ACHUPB_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR0760039.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1978 a         2029 c         2322 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZOZD010000182         8155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOZD010000182 DAZOZD010000000
VERSION     DAZOZD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.182"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..449)
                     /locus_tag="ACHUPB_003302"
     CDS             complement(<1..449)
                     /locus_tag="ACHUPB_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0760040.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHUPB_003303"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHUPB_003303"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0760041.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="ACHUPB_003304"
     CDS             complement(1788..2507)
                     /locus_tag="ACHUPB_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0760042.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="ACHUPB_003305"
     CDS             complement(2691..3017)
                     /locus_tag="ACHUPB_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0760043.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHUPB_003306"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHUPB_003306"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0760044.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="ACHUPB_003307"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="ACHUPB_003307"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0760045.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="ACHUPB_003308"
     CDS             4977..5252
                     /locus_tag="ACHUPB_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0760046.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="ACHUPB_003309"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="ACHUPB_003309"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0760047.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="ACHUPB_003310"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="ACHUPB_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0760048.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8155)
                     /locus_tag="ACHUPB_003311"
     CDS             complement(7903..>8155)
                     /locus_tag="ACHUPB_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0760049.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2159 c         1987 g         2087 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcga
//
LOCUS       DAZOZD010000183         8120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOZD010000183 DAZOZD010000000
VERSION     DAZOZD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.183"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..492
                     /gene="ftnA"
                     /locus_tag="ACHUPB_003312"
     CDS             <1..492
                     /gene="ftnA"
                     /locus_tag="ACHUPB_003312"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0760050.1"
                     /translation="KPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLRRH
                     AQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKINEL
                     AHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELSTLD
                     TQN"
     gene            complement(530..769)
                     /locus_tag="ACHUPB_003313"
     CDS             complement(530..769)
                     /locus_tag="ACHUPB_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0760051.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            960..2171
                     /gene="tyrP"
                     /locus_tag="ACHUPB_003314"
     CDS             960..2171
                     /gene="tyrP"
                     /locus_tag="ACHUPB_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0760052.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2233..2898)
                     /gene="yecA"
                     /locus_tag="ACHUPB_003315"
     CDS             complement(2233..2898)
                     /gene="yecA"
                     /locus_tag="ACHUPB_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0760053.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3094..3180)
                     /locus_tag="ACHUPB_003316"
     tRNA            complement(3094..3180)
                     /locus_tag="ACHUPB_003316"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3144..3146),aa:Leu,seq:taa)
     gene            complement(3193..3266)
                     /locus_tag="ACHUPB_003317"
     tRNA            complement(3193..3266)
                     /locus_tag="ACHUPB_003317"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3232..3234),aa:Cys,seq:gca)
     gene            complement(3321..3396)
                     /locus_tag="ACHUPB_003318"
     tRNA            complement(3321..3396)
                     /locus_tag="ACHUPB_003318"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3361..3363),aa:Gly,seq:gcc)
     gene            complement(3548..4096)
                     /gene="pgsA"
                     /locus_tag="ACHUPB_003319"
     CDS             complement(3548..4096)
                     /gene="pgsA"
                     /locus_tag="ACHUPB_003319"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0760054.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4153..5985)
                     /gene="uvrC"
                     /locus_tag="ACHUPB_003320"
     CDS             complement(4153..5985)
                     /gene="uvrC"
                     /locus_tag="ACHUPB_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0760055.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(5982..6638)
                     /gene="uvrY"
                     /locus_tag="ACHUPB_003321"
     CDS             complement(5982..6638)
                     /gene="uvrY"
                     /locus_tag="ACHUPB_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0760056.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6934..7110
                     /gene="yecU"
                     /locus_tag="ACHUPB_003322"
     CDS             6934..7110
                     /gene="yecU"
                     /locus_tag="ACHUPB_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0760057.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7097..7321
                     /gene="yecF"
                     /locus_tag="ACHUPB_003323"
     CDS             7097..7321
                     /gene="yecF"
                     /locus_tag="ACHUPB_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0760058.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7388..8110)
                     /gene="sdiA"
                     /locus_tag="ACHUPB_003324"
     CDS             complement(7388..8110)
                     /gene="sdiA"
                     /locus_tag="ACHUPB_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR0760059.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2011 a         2075 c         1905 g         2129 t
ORIGIN      
        1 aaaccagaaa tgattgaaaa acttaatgag cagatgaacc tggaactgta ctcttcactg
       61 ctttatcagc aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc tgccgcgttc
      121 ctgcgccgtc acgcccagga agagatgacg catatgcagc gtctgtttga ttacctgact
      181 gataccggca atttaccgcg tattaatacc gttgaatctc cgtttgctga atattcctca
      241 cttgatgaat tattccagga aacctataaa cacgaacaat taatcaccca gaaaattaac
      301 gaactggctc atgctgcaat gaccaatcag gactacccaa catttaattt cctgcagtgg
      361 tatgtttctg agcagcatga agaagagaaa ctgttcaaat cgattattga taaattaagc
      421 ctggcaggca aaagcggcga aggtctgtat tttatcgaca aagaactctc taccctcgac
      481 acacaaaact aatgctctcc ggcggcaggt ttactgccgc cgtttcaaat cagtgacggc
      541 aaatcttact ctgatcggtc gaaaaaccgt ctttcgaagg tataaatttg ccttttgctg
      601 ccagaaacgc taccagctct cccgctgtca tcccttctgc cgagcaggtg tgaaaacgtg
      661 cctgttcacc aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc
      721 ctgattcaat catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc
      781 aaaaaaagcc tagcgtagcg attgccgctt atgaagactt tgcgccagcg caggactgaa
      841 tgctttttat tgtacattta tatttacact atatgtaacg tcggtttgac gaagcagccg
      901 ttatgcctta acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg
      961 tgaaaaacag aaccctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggca
     1021 tgctggcaat gccgctggct gcggccggtg ttggttttag cgttacgtta atcttgttga
     1081 ttgggctttg ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg
     1141 ttccggcaga taccggtctg ggcacgctgg caaaacgcta tctgggacgc tacggtcaat
     1201 ggctgacggg cttcagtatg atgttcttaa tgtatgctct gactgcggca tacatcagcg
     1261 gtgtcggtga attgttggcc tccagcatca gcgactggac aggtatttca atgtcggcaa
     1321 ccgctggcgt gctgttgttc acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc
     1381 tggtcgattt atttaaccgt tttctgttca gcgccaaaat tatttttctg gtggtgatgc
     1441 tggtattgct gctaccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg
     1501 ggctggctct gtcagcaatc ccggtgattt ttacctcatt tggttttcac ggtagcgtgc
     1561 cgagtattgt cagctatatg gatggcaacg ttcgtaagct acgctgggtg tttataaccg
     1621 gtagtgcgat ccccctggtg gcatatattt tctggcaggt ggcaacgctt ggcagcattg
     1681 attcaacaac ctttatggga ttgctggcta atcatgctgg attaaacggg ctgttacagg
     1741 cgttacgcga aatggtggcc tctccgcatg ttgagctggc agtgcattta tttgctgatt
     1801 tagccctcgc cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt
     1861 tatttcagcg ttcaaatacc gttggtggac ggttgcaaac tggtgcaatt acctttctgc
     1921 cgccgttggc gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg
     1981 gtgtggcgct ggcggtactg gcattgatta tcccttcgct gttgacctgg caaagcagaa
     2041 agcacaatcc tcaggcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt
     2101 ttctctgtgg tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag
     2161 aagtggggtg atcagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc
     2221 caatttggcc tcttaatgca ggcagcactg cttaaatttc ttaccactac cgcacgggca
     2281 aggatcgtta cgccccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg
     2341 cggatgcgcc atccagtacg catgtagatc aagcgccgcc agtcgaatgg catctacgct
     2401 ctcttcaaac gcttctggcg acatcttttc tacccgctcg aagttttcct cagtaccgtg
     2461 cagcgcaatc gcctccagcg ctggttttaa cgaatcgggc aacgttgacc agtcagaaag
     2521 tgccacgccc cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc
     2581 aacttctcgc aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc
     2641 ggtatcggcc atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc
     2701 ccagcgcggc acatagtcag ccccacccca cacggcaacc agccactgtt ccggttcaat
     2761 ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaaggat
     2821 ggcgtggtta gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt
     2881 taacggtccc gttttcatac gcttttcctt gtggatctca actcgccagc acctatctta
     2941 catgccggtc cgtatcagag atactttttg agtggctttg ccggtgatta aaaattaagg
     3001 agggtgtaac gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg
     3061 cttattgctt tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg
     3121 cacagcgcga acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc
     3181 tcgggaagaa agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg
     3241 ctacataacc gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctt
     3301 gacgccgatg tcttttaaac tggagcggga aacgagactc gaactcgcga ccccgacctt
     3361 ggcaaggtcg tgctctacca actgagctat tcccgcattc atcaagcaat cagttaatca
     3421 cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg atgcgttgca ttctacttac
     3481 ctggcgcgat gagtcaacga tatttttcac cacttttgat cgtttgctga aaattacgcc
     3541 gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct caaatattgc aacattgacc
     3601 acagagtcag taccgcagcc acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt
     3661 tcggacgcca cagcagccat gccaacgcca ccatctgggc agtggttttc actttcccaa
     3721 tccaggagac agccacgcta ctgcgtttac ccaactccgc catccattcg cgtagcgcag
     3781 aaataataat ttcacgggcg atcatcgttg ctgccggtaa ggtcacccac cagctgtggt
     3841 aatgctcggt taccagcacc atggcgatag ccacgagaac tttatctgct acagggtcaa
     3901 ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt
     3961 cagtcaccgc cgcgacgcag aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca
     4021 gataaaagac caatacaaag aatgggataa ggatgacacg gaacagtgta agcaacgtag
     4081 ggatattaaa ttgcataatg acgggtaact atctgttgtc agtaagatta cccctatgtt
     4141 gctacagaga catcaatgtt tcaacgacca gaagatcttt tctgccagac cttgcgaaat
     4201 acccggcact tttgcaattt cctcgacgct ggcgttacgt aaaccttgca aaccgcccat
     4261 atatttcaac aacatttgcc gacgttttgg cccgacgcct tcaatggttt ccagggaact
     4321 ggtatttttg accttcgccc gttttttacg gtgcccgcca atcgcgtgat catgtgattc
     4381 atcgcgaata tgctggataa catgcagcgc gggagaatct ggcggcaaac tgaatccctc
     4441 accttccggc tcaaagaaca gcgtttccag cccagcctta cgatctgctc ctttggcaac
     4501 gccaagtagc agcggatgat ttttatccca tgagacatcc agttcggcga agacattttt
     4561 cgcctgcgca agctggcctt tgccgccgtc gataaggatc acatccggga tcttactgtc
     4621 gtcaatggct ttaccataac gccgacgcag cacctgattc atcgccgcat aatcatcgcc
     4681 cggcgtgatg cctgtaatgt tatagcgccg atactccgca cgcagcgggc cgttagcatc
     4741 aaacaccaca caggaagcga cggtttgttc gcccatggta tggctgatgt caaagcactc
     4801 catccgcttc acttccggca atttcaacac gctggcaagc gcggtcagtc gctggtgaac
     4861 ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt
     4921 cagataacgc gccctatcgc cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag
     4981 ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc
     5041 acccggtaag gtgcgcatct ggctgccttg taaatagaac tggcctacga aggtttctac
     5101 cacctcgctc agttccgtac cgccaggcac tttcgggaaa tagctgcggc tgccgagcac
     5161 tttgccctga cgaatgaaca atacgtggac acaagccatg cccgcatcga acgccacacc
     5221 aataacgtcg aggtcgtcgc cggtattgga aacgaattgt ttttcggtga cgcgtcgcac
     5281 cgcctgaatt tggtcgcgaa tacgtgcagc ttcttcaaac tccagattct ggctggcagt
     5341 ttccatacga ctaatgagtt gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg
     5401 cacatactca acctgctgag cgtattcttc ttcactcacc agtccttcaa cgcacggtcc
     5461 cagacagcgc cctatctgat attgcagaca cggacgcgag cgattgcgat aaacactatt
     5521 ttcgcactgg cgaatgggga aaatcttttg cagtagcgcc agtgtttcac gtacggcata
     5581 gccattcggg aacgggccga aatattcacc tttggcatgc ttcgcaccac gatgcatcgc
     5641 cagacgcggg tgggtatcgc cactcaggaa gataaaagga taagatttat catcgcgtag
     5701 caaaacgttg taacgcggct gatagagttt gatgtagttg tgttccagca acagcgcttc
     5761 ggtttctgta tgcgtaaccg ttacatcaat ttgctggatc tgggcgacca gcgcttcggt
     5821 tttgcgcgaa gcgaggttgc tgcggaaata gctggaaagc cgttttttca ggtctttcgc
     5881 tttgccgaca tagataaccg taccaccagc atcgtacatg cgataaacgc ctggctggca
     5941 ggttacggtt tttaaaaacg cttttgcgtc aaactgatca ctcactgact tgataatgtc
     6001 tccgcattac acagaccatg gcgaattgcc aggtgagtca gctcaacatc gccatgaatg
     6061 tttagtttac tgaacatacg atagcggtag ctgttcaccg ttttcggact gagattgagc
     6121 tgttctgaga tctcattgac cttctggccc ttggtgatca tcagcataat ctgcaattca
     6181 cgttcagaca aactggcaaa tgggctttct gttttttctg gttcgatctg gcttaacgcc
     6241 atttgttgag cgatatcaga agcaatgtaa cgctgccctg aatagacaga acgaatcgca
     6301 ctcacgactt cctgcggagc cgcgcctttg ctgaggtagc ccgcagcacc ggcctgcatg
     6361 actttcgctg gtaaagggtt ttctgtatgg acggtaagca tgatgatttt gacatcagct
     6421 gtggaacgcg cgattttacg cgtcgcctca agaccgccaa tgcccggcat actcatgtcc
     6481 attagcacca cgtcaacggc atttgtccgg caccacttaa cggcgtcttc accgcacgat
     6541 gcctcaccga cgacttttat accctttata tcttccagaa tgcgtcgtat ccctgcgcgc
     6601 accagttcgt ggtcatcaac aagtagaacg ttgatcaaag gaatatctcc agaaataggg
     6661 ataacgctac tgatagttag tcattcgtat attagcggtt tttattgcaa ctttgaaacg
     6721 ttaaaaatgg tgggtattcg atttttctct cgtttttgta aattcgattg tccacagtta
     6781 tggtcacgcc cgtcaccaac ggcttccggg atcgtttcac agtaagcccg gcgatgaaat
     6841 gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt
     6901 aattaacccg aacaatacgg gcaaaaaaca ttcatgataa aaatatttat tggtcattat
     6961 attaacgcat ttaatagcat tgttgacgtc acgctcaaaa aacaaccact gctattttta
     7021 gcaaagctta tggtatactc cgccgcctta acatttttca ccgcaaattt tcattgcaac
     7081 atgacgagga aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact
     7141 ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg atgctacagg cgatgggaca
     7201 agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga
     7261 aacccaggct gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta
     7321 atataaaacc ggctgagagc gtgcctttca gccgattttt gcatctggca cgcagaaaga
     7381 aaagcgatca aattaagcca gtagcggccg cgtaacaggc cacctgggtc ttatttggtg
     7441 cattgatttt tttctgcatg tttttctgat ggaaattgac cgtattctca gagattgaca
     7501 aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga atttcttttt
     7561 cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag
     7621 ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg
     7681 gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga tttggcagca
     7741 ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc cataatggct
     7801 gtgcttcgct gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt
     7861 tcagcacagg atcaattgcg agaaaatttt ttgcctgata ataactaacc cacgactcag
     7921 ggtaattggt gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata
     7981 acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg tagacctctt
     8041 ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg
     8101 tatcctgcat agtaaaccgc
//
LOCUS       DAZOZD010000184         8083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOZD010000184 DAZOZD010000000
VERSION     DAZOZD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.184"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            85..1581
                     /gene="ravA"
                     /locus_tag="ACHUPB_003325"
     CDS             85..1581
                     /gene="ravA"
                     /locus_tag="ACHUPB_003325"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0760060.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUPB_003326"
     CDS             1575..3026
                     /gene="viaA"
                     /locus_tag="ACHUPB_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0760061.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUPB_003327"
     CDS             complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHUPB_003327"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0760062.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUPB_003328"
     CDS             4175..4633
                     /gene="asnC"
                     /locus_tag="ACHUPB_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0760063.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUPB_003329"
     CDS             4723..5166
                     /gene="mioC"
                     /locus_tag="ACHUPB_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0760064.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUPB_003330"
     CDS             5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHUPB_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0760065.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7498..>8083
                     /gene="rsmG"
                     /locus_tag="ACHUPB_003331"
     CDS             7498..>8083
                     /gene="rsmG"
                     /locus_tag="ACHUPB_003331"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0760066.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDG"
BASE COUNT         2016 a         2075 c         2076 g         1916 t
ORIGIN      
        1 ctttgagact attttagcca ccagcaagaa acgtctatac tcgcaattta cgcagaactt
       61 ttgacgaaag gacgccactt cattatggct caccctcatt tattagcgga aagaatttcc
      121 cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat ccgcttgtgt
      181 ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa
      241 agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg
      301 atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca ggcgctaaaa
      361 gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga aattgtcttt
      421 ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac cgccattaac
      481 gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct gctggtggcg
      541 gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga ccgcatgctg
      601 attcgtctgt ggttagataa agtgcaggat aaagcgaatt tccgctccat gctgaccagt
      661 caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga tgaagaatat
      721 gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt tgagctgatt
      781 tttatgctgc gcctgcaact ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc
      841 tggaaaaaag cgatccgatt attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt
      901 gccccggtag atctcatttt gctgaaagat tgcctgtggt atgacgcgca aagcctgaat
      961 ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca gcaagggatg
     1021 ttgactcgcc ttggcgcgat tgtgcaacgt cacctgcaac tccagcagca acaaagcgat
     1081 aaaacagcct taacggtaat tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa
     1141 cttcctgtta acgttactgc ttccactctg actctactgc tgcaaaaacc gttaaaactg
     1201 catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca gtggctgagc
     1261 aaaggtggtg aaattcgcgg taaactgaac ggtatcggct ttgcccagaa actgaatctg
     1321 gaagttgata gcgcccaaca tcttgtcgtt cgcgatgtaa gtttacaagg cagtacgctg
     1381 gcacttcccg gttcatcggc tgaaggtctg cctggtgaaa taaaacaaca actggaagag
     1441 cttgaaagcg actggcgcaa gcaacacgct ttattcagcg aacagcaaaa atgtctgttt
     1501 atccctggcg actggttagg tcgcattgaa gccagcctac aggatgtcgg tgcacagatt
     1561 caccaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc gtcagcgaag
     1621 agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg gcggtcttct
     1681 ttgaaaaatt cccacgcctg aaggcggcaa tcactgatga tgttccccgc tggcgtgagg
     1741 cgctgcgcag tcggctgaaa gatgcccgag tcccgccgga actcaccgaa gaggtgatgt
     1801 gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta ccacagatcc
     1861 tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag ttggttgatg
     1921 ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg cgtttaagtc
     1981 tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc gaacaactgt
     2041 tgagtgaagt tcaagaacgc atgacgctga gcggacaact tgaaccgatt ctcgcagata
     2101 acaatactgc agctggtcgt ctgtgggata tgagcgccgg tcagcttaaa cgtggcgact
     2161 atcagttgat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg
     2221 cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg
     2281 aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc
     2341 aacaaagcga tgatatttta cgtcttctgc cgccagaact ggcgacacta gggataacgg
     2401 aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc
     2461 acggtgagtc gtggcgtgaa aaagtgatcg aacgtccggt ggtacataaa gattacgatg
     2521 aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg ggcggcttta
     2581 atgaacagtg tgcgaaagcg ttctgcctgg ccttgatgcg cattgctctc gctgaaaacc
     2641 ggcgctgcta tattatgcta ttttccaccg agatcgtccg ttatgagctt tcaggccccc
     2701 aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc accgatcttg
     2761 ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt gatgccgatg
     2821 cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg agtaaagtga
     2881 aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca
     2941 aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg atgcgaagcc
     3001 gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgt tctcgcgaac
     3061 agcagctggc catacaccac actgaacctg gccgatatgc ggcagttgca gcagcagcat
     3121 ggtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac cgcgcagcag
     3181 cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca actgatgctt
     3241 caacgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat cttccagtac
     3301 agggttccac accagaatat cgccgttcag acccgcatga cccagctctg acggggtgct
     3361 ccagtcatca taatccggtg cgcgcacgtc gtggcgatga ccatcgctca gcttgccgcc
     3421 aatcccgaca aggaataccg cgccaagatc tttcgctatc gcccgctcac gccctttggc
     3481 atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgga tctgatccgg
     3541 caggaacggt gccaggccaa actcttcgct aaccgcagct tcggttgctt taattcccgc
     3601 ccagatcgcc tctaccgtgc ttttcagagt cgagaattga cgctcaccgt cgcccattac
     3661 gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac ggtcttcatc
     3721 ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc
     3781 taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg catcaggcag
     3841 agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct gcgtgccatc
     3901 ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt cttccagttg
     3961 gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt
     4021 cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc gacaaacatg
     4081 caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag aaggctaaaa
     4141 tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga tcgacaatct
     4201 ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg ccgaactggc
     4261 gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa tgaagcaggc
     4321 ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt atgacgtagg
     4381 ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc tggcaaagct
     4441 ggaaagcctt gctgaagtca ctgaagccta ttacacaaca ggccactaca gcatctttat
     4501 aaaagtaatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca agatccaaac
     4561 aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga tcatgcgtac
     4621 catcaagcca taatcggctt ttttaatccc atacttttcc acaggtagat cccaacgcgt
     4681 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
     4741 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
     4801 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
     4861 tcggggatct ggctgattat cagctccacc cacggtgccg gagatattcc ggacaacctt
     4921 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
     4981 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctatcga taaactcgag
     5041 gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat caacattctt
     5101 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
     5161 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
     5221 ccggtagtta tccaaggaac aacggttgtt cagtttttga gttgtgtata acccctcatt
     5281 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
     5341 gttgttgatc ttaaaagccg gatccttgtt atccacaggg tagtgcgatc ctaataagag
     5401 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
     5461 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
     5521 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
     5581 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
     5641 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
     5701 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
     5761 gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg accggcggtt
     5821 cgcgctaccc gagctcaggc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
     5881 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
     5941 gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg
     6001 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
     6061 ggtcgtgctg gtgatccgcc gtccattccg ctttctcgcc gtttgcgtga actgccgctg
     6121 cgcgttggtc gtctgaaaac cgggacacca ccgcgtattg atgctcgaac tatcgacttt
     6181 agtgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggcaat
     6241 gcgtcccagc atccccagca ggtgccgtgt tatatcactc ataccaacga gaaaacccat
     6301 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtacg caggggtgat cgaaggtgtc
     6361 ggcccacgct actgcccatc gatcgaagac aaagtcatgc gcttcgccga cagaaatcag
     6421 catcagatct tccttgaacc ggaagggctg acctctaacg aaatttatcc gaacggtatc
     6481 tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctccatgca ggggatggaa
     6541 aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga tccacgcgac
     6601 ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg tcagattaac
     6661 ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggtcggtct taacgctgcc
     6721 cgtctgtctg ctgacaaaga aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta
     6781 ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc
     6841 gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt
     6901 cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat
     6961 atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca
     7021 gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg tgaagatctg
     7081 ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg
     7141 ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga aggttatatc
     7201 gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg
     7261 acactggatt accgccaggt atccggtctt tctaacgaag tgatcgccaa acttaacgat
     7321 cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc tgcggctatc
     7381 tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt
     7441 aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg
     7501 ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttaccga tcaccagaaa
     7561 aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta caacctgact
     7621 tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat tgtggtggca
     7681 ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact gccaggcatt
     7741 ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct tggtaaacgc
     7801 gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga gccagtacag
     7861 agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag ccgcgctttt
     7921 gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca aggccgtttc
     7981 tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga agaatatcag
     8041 gtcgaatcag tggttaaact tcaggttcca gctctggatg gcg
//
LOCUS       DAZOZD010000185         8082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOZD010000185 DAZOZD010000000
VERSION     DAZOZD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.185"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            32..553
                     /gene="fecI"
                     /locus_tag="ACHUPB_003332"
     CDS             32..553
                     /gene="fecI"
                     /locus_tag="ACHUPB_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR0760067.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            550..1503
                     /gene="fecR"
                     /locus_tag="ACHUPB_003333"
     CDS             550..1503
                     /gene="fecR"
                     /locus_tag="ACHUPB_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR0760068.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1590..3914
                     /gene="fecA"
                     /locus_tag="ACHUPB_003334"
     CDS             1590..3914
                     /gene="fecA"
                     /locus_tag="ACHUPB_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR0760069.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            3959..4861
                     /gene="fecB"
                     /locus_tag="ACHUPB_003335"
     CDS             3959..4861
                     /gene="fecB"
                     /locus_tag="ACHUPB_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR0760070.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            4858..5856
                     /gene="fecC"
                     /locus_tag="ACHUPB_003336"
     CDS             4858..5856
                     /gene="fecC"
                     /locus_tag="ACHUPB_003336"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR0760071.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            5853..6809
                     /gene="fecD"
                     /locus_tag="ACHUPB_003337"
     CDS             5853..6809
                     /gene="fecD"
                     /locus_tag="ACHUPB_003337"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR0760072.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            6810..7577
                     /gene="fecE"
                     /locus_tag="ACHUPB_003338"
     CDS             6810..7577
                     /gene="fecE"
                     /locus_tag="ACHUPB_003338"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR0760073.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1729 a         2332 c         2283 g         1738 t
ORIGIN      
        1 tacgtgataa ttaacttttg atgcactccg catgtctgac cgcgccacta ccacagcttc
       61 cttaacattc gagtcgcttt atggcacaca tcacggctgg ttgaaaagct ggctgacgcg
      121 caaactccag tctgcttttg atgcagatga cattgcccag gacacttttt tgcgggtaat
      181 ggtcagcgaa acgctctcga cgatccgcga tcctcgctcc ttcctctgca ctatcgccaa
      241 acgcgtgatg gtggacctgt ttcgccgaaa cgcgctggaa aaagcgtatc tggagatgct
      301 ggcgcttatg ccggaggggg gagcgccttc acctgaggaa cgcgaaagcc aactcgagac
      361 cctacaactc ctcgacagca tgctggacgg gctaaacggc aaaacacgtg aagcgtttct
      421 gctttcgcaa ctggatggtc tgacatacag cgagattgcg cacaaactcg gtgtttccat
      481 cagctccgtg aaaaaatacg tggcgaaagc cgtcgagcac tgcctgctgt tccgtctgga
      541 gtatgggtta tgaatccttt gttaaccgat tcccgccgtc aggcgctgcg ttcagcttcc
      601 cactggtatg ccgtgctaag cggcgagcgc gtcagcccac aacaggaagc gcgctggcaa
      661 cagtggtatg aacaggatca ggataaccag tgggcctggc agcaggttga aaacctgcgc
      721 aaccagcttg gcggtgtgcc tggcgacgtt gccagccggg cgttgcacga tacccgcctc
      781 acccgccgtc acgtgatgaa aggattactg ctgttgctcg gcgctggcgg aggctggcag
      841 ctctggcagt cggaaaccgg cgaaggtctg cgggcagatt accgcaccgc caaaggcaca
      901 gtcagccgtc agcaactgga agatggctcc ctgctcacgc tgaataccca aagcgcggcg
      961 gatatgcgtt ttgatgcgca tcagcgcacc gtccggctct ggtacggtga aatcgccatt
     1021 actaccgcga aagatgccct gcaacgcccc ttccgtgtcc tgacccgtca gggccagctc
     1081 actgctttag ggacagaatt taccgtccgc cagcaggata atttcacgca gcttgacgtg
     1141 cagcagcacg ccgtggaagt gcttctcgcc agtgcccccg cgcaaaaacg catcgtgaac
     1201 gctggtgaaa gcctgcagtt cagcgcctct gagtttggcg cagtgaaacc gctggatgac
     1261 gagagtacaa gctggacgaa ggacatcctg agcttcagcg ataaaccgct gggtgaggtg
     1321 atagccacgc taacccgtta ccgcaacggc gtgctgcgct gcgatcccgc cgttgccggg
     1381 ctgcgcctga gcgggacgtt cccgctgaaa aataccgatg cgatcctgaa cgttatcgcg
     1441 caaacgcttc ccgttaaaat tcagtctatt acgcggtact ggataaacat ttcaccactg
     1501 taaggaaaat aattcttatt tcgattgtcc tttttaccct tctcgttcga ctcatagctg
     1561 aacacaacaa aaatgatgat ggggaaggta tgacgccgtt acgcgttttt cgtaaaacaa
     1621 cacctttggt taacgccatt cgcctgagcc tgctgccgct ggccggtctc tcgttttccg
     1681 cttttgctgc acaggttaat atcgcaccgg gatcgctcga taaagcgctc aatcagtatg
     1741 ccgcacacag cggatttacc ctctcggttg acgccagcct gacgcgcggc aagcagagca
     1801 acggcctgca cggcgattac gacgtcgaga gcggcctgca acaactgctg gacggcagcg
     1861 gactgcaggt aaaaccgctg ggaaataaca gctggacgct ggagcccgcg cccgcaccaa
     1921 aagaagatgc cctgaccgtg gtcggcgact ggctgggtga tgcgcgtgaa aacgacgtat
     1981 ttgaacatgc tggcgcgcgt gacgtgatcc gccgtgagga tttcgccaaa accggcgcaa
     2041 ccaccatgcg tgaggtactt aaccgcatcc ctggcgtcag cgcgccggaa aacaacggca
     2101 ccggcagcca cgacctggcg atgaactttg gcatccgggg cctgaacccg cgcctcgcca
     2161 gccgctcgac cgtcctgatg gacggcatcc ccgtcccctt cgccccttac ggtcagccgc
     2221 agctttcact ggctcccgtt tcgctcggca acatgaatgc cattgacgtg gtacgcggtg
     2281 gtggtgcggt gcgttacgga ccgcagagcg tgggcggcgt ggtgaacttt gttacccgtg
     2341 ccattccgca ggactttggt atcgaggcgg gcgtggaagg tcagctcagc ccaacctctt
     2401 cacaaaacaa cccgaaagag acgcacaacc tgatggtggg cggcacagcg gacaacggtt
     2461 ttggcaccgc gctgctctac tccggcacgc gcggcagtga ctggcgcgag cacagcgcca
     2521 cccgcatcga cgacctgatg ctgaaaagca aatatgcgcc ggatgaggtg cacaccttca
     2581 acagcctgct gcaatattac gacggtgaag ccgacatgcc cggtggcctg tctcgcgcgg
     2641 attacgacgc cgatcgctgg caatccaccc gcccgtatga ccgcttctgg ggtcgtcgca
     2701 agctggcgag cctgggctac cagttccagc cagacagcca gcataaattc aacattcagg
     2761 ggttctacac ccaaaccctg cgcagcggct acctggagca aggcaaacgc atcaccctct
     2821 cgccgcgtaa ctactgggtg cgcggtattg agccacgcta cagccagatc tttatgatcg
     2881 gcccttccgc gcacgaagtg ggcgtgggct atcgctatgt gaatgaatca acgcatgaaa
     2941 tgcgttacta caccgccacc agcagcgggc agttgccgtc cggctcaagc ccttacgacc
     3001 gcgatacgcg ttccggcacc gaggcgcacg cctggtatct ggatgacaaa atcgacatcg
     3061 gcaactggac catcacgccg ggtatgcgtt tcgaacatat cgagtcatac cagaacaacg
     3121 ccatcacagg cacgcacgaa gaagtgagct ataacgcacc gcttccggcg ttgaacgtgc
     3181 tctatcacct gactgacagc tggaatcttt atgcaaacac tgaaggctcg ttcggcaccg
     3241 tacagtacag ccagattggc aaggctgtgc aaagcggcaa tgtggaaccg gaaaaagcgc
     3301 gaacctggga actcggtacc cgctacgacg acggcgcgct gacggcggaa atggggctgt
     3361 tcctgattaa ctttaacaat cagtacgact ccaaccagac caacgacacc gtcactgcac
     3421 gtggcaaaac gcgccatacc gggctggaaa cgcaggcacg ttacgatctg ggtacgctaa
     3481 cgccaacgct tgataacgtt tccatctacg ccagctatgc gtatgtgaac gcggaaatcc
     3541 gcgagaaagg cgacacctac ggcaatctgg taccattctc cccgaaacat aaaggcacgc
     3601 tgggcgtgga ctacaagcca ggaaactgga tgttcaatct gaacagcgat ttccagtcca
     3661 gccagtttgc ggataacgcc aatacggtga aagagagcgc cgacggcagt accggccgca
     3721 ttcccggctt catgctctgg ggcgcacgcg tggtgtatga ctttggcccg cagatggcag
     3781 atctgaacct ggcattcggt gtgaaaaaca tcttcgacca ggactacttc atccgctctt
     3841 atgacgacaa caacaaaggc atctatgcag gccagccgcg cacgctgtat atgcaggggg
     3901 cgttgaagtt ctgatgtgtt aacgcccggc ttgccgggct tttagctgga atgtgattat
     3961 gttggcattt atccgttttc tttttgcagg cctgctgctg gtgatcagcc acgcctttgc
     4021 cgccacggtt caggacgaac acggcacgtt tacactcgaa aaaacgccac aacggattgt
     4081 ggtgctggaa ctctcgttcg ccgatgcgct ggccgccgtg gacgtcagcc cgatcggtat
     4141 tgccgacgat aacgatgcaa aacgcatcct gcccgaagtg cgtgcgcacc tgaaaccgtg
     4201 gcagtccgtc ggaacgcgcg cgcagccgag cctggaagcc attgccgctc tgaaaccaga
     4261 cctgatcatt gccgacagca gtcgccatgc gggggtttac atcgccttgc agcaaatcgc
     4321 gtcggtactg ctgcttaagt cccgcaacga aacctacgct gaaaatttgc aatctgcggc
     4381 tatcatcggc gaaatggtgg gtaaaaagcg agagatgcag gcacgtctgg aacaacataa
     4441 agagaggatg gcgcagtggg ccagccagct tcccaaaggg acacgcgtgg cctttggcac
     4501 atcacgcgaa cagcaattca acctgcatac tcaggagacc tggaccggca gcgtgctggc
     4561 ctctctgggg ctgaacgttc ccgctgcgat ggcgggtgcg tccatgccgt ccatcggcct
     4621 ggagcaactg ctggcggtca atcctgcctg gctgctggtt gcccactatc gcgaagagag
     4681 cattgttaaa cgctggcaac aagatccgct ctggcagatg ttaaccgccg cgcagaagca
     4741 gcaggttgct tcggtcgaca gtaacacctg ggcgcggatg cgcggtattt ttgctgcaga
     4801 gcgtattgcc gctgacacgg taaaaatctt ccaccatcag ccgcttaccg ttgtgaaatg
     4861 accgcgataa aacacccggt gctgctgtgg gggcttcccg ttgcagcact tattattatt
     4921 ttctggctga gtctgttttg ctactcggcc attcctgttt ccggagcaga tgcaacccgc
     4981 gccctgctgc ctggacacac gccaacgcta ccagaagcgc tggtgcaaaa ccttcgtttg
     5041 ccacgaagcc tggtcgccgt tctgatcggc gcaagcctgg cgctcgcggg cacgctgctg
     5101 caaaccctga cccacaaccc aatggcctct ccttcactgc tcggcattaa cagcggcgcg
     5161 gcgctggcta tggcgcttac cagcgcgctg agtccgacgc cgattgcagg ctattctctg
     5221 tcgttcatcg cggcatgcgg gggcggcgtg agctggctgc tggtcatgac cgcaggaggc
     5281 ggatttcgtc atacccatga cagaaacaaa ctgatcctcg cgggtatcgc gctgtcggcc
     5341 ttttgtatgg gccttacccg catcaccctg ctgctggccg aagatcatgc ttacggcatc
     5401 ttttactggc tggctggcgg agtgtcccac gcccgctggc aggatgtctg gcagctcttg
     5461 ccggtggtgg tcactgcagt ccctgtcgtg ttgctgctgg cgaatcaact gaacctgctc
     5521 aacctcagcg acagcaccgc ccatacgctg ggagtgaacc tgacgaggct acgtttggtc
     5581 atcaatatgt tagtgctgct tctggttggt gcgtgcgtca gcgtggcagg tccggtggcg
     5641 tttatcggtc tgctggtgcc acatctggcg cgcttctggg caggcttcga tcagcgcaac
     5701 gtactgccag tgagcatgct gctgggggcc acgctgatgc tgctggcaga tgtactcgca
     5761 cgcgcgctgg ccttccccgg agatctgccc gcaggcgcag tgctggcgct gattggcagc
     5821 ccttgctttg tctggcttgt gaggaggcga ggatgaaaat tgcgctggtt attttcatca
     5881 cccttgccct ggcgggctgt gcgctgttat cactccatat gggagtgatc cccgtgccgt
     5941 ggcgcgcgct gctgaccgac tggcaggccg gacgcgagca ttattatgta ttgatggagt
     6001 accgactgcc gcgcttgctg ctggcactgt ttgtcggtgc agccctcgcc gtggcgggcg
     6061 tgctgataca ggggattgtg cgcaaccctc tggcatcacc ggatattctc ggcgttaacc
     6121 atgccgccag cctggcctct gtgggggctc tacttcttat gccgtcactg cccgtgatgg
     6181 tgctgccgct gctggccttt gcgggcggca tggcggggtt gatattgctg aagatgctgg
     6241 caaagaccca ccagccgatg aagctggcac tcaccggcgt ggcgctttct gcatgctggg
     6301 ccagcctgac ggattatctg atgctctcgc gcccgcagga tgtgaacaac gccctgctgt
     6361 ggctgaccgg cagcttatgg ggccgtgact ggagctttgt gaagattgcc atcccgctga
     6421 tgattttatt tctgccgctg agcctgagtt tttgccgcga tctcgacctc cttgcactcg
     6481 gcgatgcgcg cgccaccacg ctcggtgtgt cggtgcccta tacccgattc tgggctttgt
     6541 tactagctgt cgccatgaca tctaccggcg tggccgcctg cggcccgatt agctttattg
     6601 gtctcgtggt gccgcatatg atgcgtagca tcaccggtgg acgtcaccgc agactgctgc
     6661 ctgtttcggc cctgacaggt gcgttgctgt tggtggttgc cgatctgctg gcgagaatta
     6721 ttcatccccc actggagctc ccggttggcg tgctgaccgc cattatcggt gcgccgtggt
     6781 ttgtctggtt gcttgtgaga atgcgataaa tgactttacg aactgaaaat ctgacggtca
     6841 attacggaac agacaaggta cttaacgacg tttcactctc actgccaacg gggaagatca
     6901 ccgccctgat cggtcctaac ggttgcggga aatcgacgct gttaaactgt ttttcgcggc
     6961 ttttaatgcc gcagtctggc accgtatttc tcggcgataa tcccataaat atgctctcat
     7021 cgcgccagtt ggcccgcagg ctttcgctgc tgcctcagca ccatttaacg ccagagggga
     7081 tcacagtcca ggagctggtt tcgtatggtc gtaatccctg gctgtcactc tgggggcgtc
     7141 tctccgctga agacaatgca cgagttaatg tcgccatgaa ccagacccgg atcaatcatc
     7201 ttgccgttcg tcggttaacc gagctttccg gcggtcagcg ccagcgcgca tttctggcaa
     7261 tggtcctggc ccagaatacg cccgttgtat tacttgatga gccaaccacc tatcttgata
     7321 tcaatcacca ggtggacctg atgcggttga tgggcgaact ccggactcag gggaaaacgg
     7381 tggtcgctgt gctgcacgac cttaatcagg ctagccggta ctgcgatcaa ctggtggtaa
     7441 tggcaaacgg acatgttatg gcgcaaggca caccagaaga ggtgatgacc ccaggattgc
     7501 tgagaacagt attcagcgtg gaagcggaaa tacaccccga gccggtatct ggcaggccga
     7561 tgtgcctaat gaggtagatt gcacaggccg taagaaccaa accacgactg aatgaaactg
     7621 gactggcgcc agcaagcctg ttcagactgg ggctgaactt ttccggactc tgaaagatta
     7681 ccaatactca tcgtccatcc gcttgcttta ggctgacagg ttcataatca acgcaaacca
     7741 gagctgtaca ggcttgggcg cggctttcaa accagtcgtg atcacggcaa tcaattttga
     7801 actctgctta acggacattt ctgtataacc cttacggcaa cgaaaaacgc gaagttaaaa
     7861 ttttagaaac ccaaaaacgt gacatgacta agtttagatt tcaggggggg gagatcaaaa
     7921 aatttcgctc tgtgccagag cggacattca cggagctggt tcattaccaa tgaggttggg
     7981 cttttgagga taaatcaatg atcagacgcc aacgtaaatc aaaagcaacc ctggaacgga
     8041 attgctaatc cagtttctga ccatcgattt ttctaaaaag tg
//
LOCUS       DAZOZD010000186         8068 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOZD010000186 DAZOZD010000000
VERSION     DAZOZD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.186"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            155..2398
                     /gene="pdeF"
                     /locus_tag="ACHUPB_003339"
     CDS             155..2398
                     /gene="pdeF"
                     /locus_tag="ACHUPB_003339"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0760074.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2437..3978)
                     /gene="ppx"
                     /locus_tag="ACHUPB_003340"
     CDS             complement(2437..3978)
                     /gene="ppx"
                     /locus_tag="ACHUPB_003340"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0760075.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3983..6049)
                     /gene="ppk1"
                     /locus_tag="ACHUPB_003341"
     CDS             complement(3983..6049)
                     /gene="ppk1"
                     /locus_tag="ACHUPB_003341"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0760076.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6220..6858)
                     /gene="purN"
                     /locus_tag="ACHUPB_003342"
     CDS             complement(6220..6858)
                     /gene="purN"
                     /locus_tag="ACHUPB_003342"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0760077.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6858..7895)
                     /gene="purM"
                     /locus_tag="ACHUPB_003343"
     CDS             complement(6858..7895)
                     /gene="purM"
                     /locus_tag="ACHUPB_003343"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0760078.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
BASE COUNT         1967 a         2010 c         1973 g         2118 t
ORIGIN      
        1 agggttattt tgtttgatat atatcaattg gaactttcat atgacactta gaatcaatgc
       61 tctctatagt gatgaataat catcattcga agtcaggtgg gatgcctgtc tgaatacacc
      121 tccttcagga tgtgggggat tctgctgagc atctatgaaa ctgaatgcaa cttatataaa
      181 aatgcgtgat aaatggtggg ggcttccgct gttcctgcct tctttaatct tgcccatttt
      241 cgcccacatt aatactttcg cgcatatttc ttccggtgag gtttttctct tttatctgcc
      301 tctggcactg atgatcagca tgatgatgtt tttcagctgg gcggcattgc cagggatcgc
      361 cttagggatt tttgtccgca aatatgcaga gctgggtttt tacgaaacgc tatcattaac
      421 ggctaatttt attatcatta tcattctctg ttggggcggt tacagggtct ttactccccg
      481 gcgtaacaac gtttcacatg gtgatacccg tttaatttcc cagcgtatat tctggcagat
      541 tgtgtttcct gcaacgctgt ttctgatact tttccagttt gctgcatttg taggattact
      601 ggcgagcaga gaaaatctgg tcggtgtcat gccttttaac ctcgggactt taatcaatta
      661 tcaggcctta ctggtgggta atctgatcgg tgtcccgctg tgctacttca tcattcgggt
      721 agtgcgaaat ccattttatt tacgtagcta ttattcgcaa ttaaaacagc aggttgatgc
      781 caaagtcacc aaaaaagagt tcgcgctctg gctactggca ttaggtgctt tattgttgct
      841 gttatgcatg ccgttaaatg aaaaaagcac aatttttagc accaattata ccttgtcatt
      901 attgctgccc ctgatgatgt ggggagcgat gcgctatggt tataagctga tttcgctgct
      961 ctgggcggtc gtgttgatga tcagcatcca cagctatcaa aattacattc ccatttatcc
     1021 tggctatacc acgcagctga ccataacctc ctccagttat ctggtattct cttttattgt
     1081 caattatatg gctgtactgg caacccgtca gagagcggta gtcagacgca ttcagcggct
     1141 tgcgtatatg gacccggtgg ttcatctgcc aaatgttcgc gccctgaatc gcgcgttgcg
     1201 tgatgctccc tggtctgcgc tttgttattt acgcatccct ggcatggaaa tgctggttaa
     1261 gaactatggc atcatgctgc ggattcaata caagcaaaaa ctttctcact ggctgtcacc
     1321 cttgctggaa ccgggtgaag atgtttatca gctttcgggt aacgatctcg cgctgcgact
     1381 gaatacagaa tcgcaccagg agcgcattac cgcactggat agtcatctca agcaatttcg
     1441 tttcttttgg gatgggatgc cgatgcaacc gcagattggc gtcagttact gctatgtgcg
     1501 ctcgccagtg aatcatatct acctgctgct gggagagcta aatacggtcg ccgaactttc
     1561 catcgtgacc aacgccccgg aaaatatgca gcgtcgcggg gcaatgtatt tgcaacgcga
     1621 attgaaagat aaagtcgcga tgatgaatcg actacagcag gcgctggaac acaaccattt
     1681 tttcctgatg gcccagccga ttaccggtat gcgtggtgat gtttaccatg aaattcttct
     1741 gcgcatgaaa ggtgagaatg atgaactgat cgtccccgat agcttcttgc cggtcgcgca
     1801 cgaatttggt ttatcgtcga gtatcgacat gtgggtcatt gagcatacgc tgcaatttat
     1861 ggctgaaaac agagcgaaga tgcccgctca ccgttttgct attaatctgt ctccaacctc
     1921 ggtatgtcag gctcgttttc ctgttgaagt cagtcagttg ctggctaaat atcagattga
     1981 agcgtggcaa cttatttttg aagtcaccga aagtaatgct ctgaccaatg ttaagcaggc
     2041 gcaaatcacc ttgcagcatc ttcaggaatt aggctgccag attgcgattg atgatttcgg
     2101 caccggctac gccagctatg cacggcttaa aaatgtgaat gccgatctgc ttaaaattga
     2161 cggcagcttt atccgcaata ttgtgtcaaa tagtctggat tatcagattg tggcgtcgat
     2221 ttgccatctg gcgcgaatga agaaaatgcg ggtagtggca gagtacgttg aaaacgaaga
     2281 gatccgcgag gcggtgctct ctttggggat cgattatatg cagggttatc ttattggtaa
     2341 gccgcagccg ttaattgata cgttgaatga aatcgaaccc attcgcgaaa gtgcctgaat
     2401 aatgcgggcc gacatttctc gtcggcccgc aaagcattaa gcggcgattt caggtgtact
     2461 ttcttcttca attttcaacc gccagccagc cacgccttcc cagtattctt gctccttttc
     2521 cagatcaagc agtaccaacg cattctgact aaaccagtca tgcgggaaac gcagtgtcca
     2581 gtgactgtca tcagtaatca gtgtcaatgt tggcggtgtg gtggttgcct gacgttgatt
     2641 gttgagtaat acgccaaggc gcaatagctg aatcagtggc aggaactgtt tcttcttaaa
     2701 caaggtaaag cgcggcagat cgtcgagttt aatcgcttta cggtgatagc gcaccagtgt
     2761 cgccatcatc agctgctgtt cctgattaaa acccggcaag tcactgtttt gcagaatata
     2821 agcggagtgg cgatgcaaac cgctgtggtt gatattcaac ccgacctcat gcagcatggc
     2881 ggcccatcgc agtagcgcct ccagttgcgg atgcgccagc ttcggttgct gttcccgcca
     2941 ctgttcgtac atttgcatag tggtatccag cacccgtcgg gcctgttcgc tgtcgatgtg
     3001 atactggttg gcgaggctgc tggcggtgcg actacgcaca tcctgatgac ggaaacgtcc
     3061 ttccatttcg tacagtacgc cttcgcgtaa cgccccgtca gaaaggcgca gttcacggat
     3121 ggctaaagca tcaaacacac cgcataaaat cgccagtcca ggaacgaaga ctgttttccg
     3181 ctcttcggaa agacccggta aactcagcga tgcgaaatta cggtgacgta aaacttcttt
     3241 taccagtttt tccagacgtt ccggggtaat tatcccgtct ttctcgccca tttccattaa
     3301 cacttcatgg gcggctttta tggtaccgga agcgcccatt gccacgttcc agccctgaat
     3361 acggaattgc caggttaaag tttccagttt ttgtgctgcc gccatacgag cgcgctgaaa
     3421 attctcttta ttgatgaccc cgccagggaa gtaaagctgg gcaaagctga cacaacccat
     3481 ccggcggctt tcaacgagaa tcggttcgaa attttcacca atcaccagtt ccgtagatcc
     3541 gccgccaata tcaataacca gtttgcgacc tttctccggt tgggtatgtt ccacgcccat
     3601 aaaaatcaga cgggcttctt cattaccgga aataatttca atcgggtagg gaatgacctt
     3661 ttccgcgcgt ttcagaaagt cagtggcgtt cagcgcctga cgcagcgtat gggtgccgac
     3721 tatacagacg ctggcaggag aaaacccttg tagccgttcg gcaaacagcg acagacagtt
     3781 caaaccgcgc gtcattgcct cttcactcaa catattatct ggccccaggc cgtccgccag
     3841 atgcacccgc tgtttcaggc ggccaataat ctgcatggca ccatctacca cacgggctat
     3901 gaccatgtga aaactgtttg aaccaagatc gaccgcagca aactcctgcg gacgagggga
     3961 tttatcgtgt attggcatag ggttattcag gttgttcgag tgatttgatg tagtcataaa
     4021 tcgccaactg cgcccgtact ttgcggcgat tgccgcgggg aacgtagcga ttactgagtt
     4081 ctttatcgat ataacgagct ttgaccgtat cgctgaacaa tatgtcgatg atgtccagca
     4141 cccgctgctt caggcgcgga tcgagcagcg gtgtcgccac ttcaatacga taatcaatat
     4201 tgcgcgtcat ccagtcggcg gaagaaaggt agaccttttt atcgccgcca ttttcaaaaa
     4261 tataaacccg gtcatgttca aggtaacggt caacaatact gatggcacga atgttgtcgc
     4321 taatgccttc cagattgggg atcagcgaac acattccgcg aaccagcaga ttaaccggta
     4381 cgccggagct ggaggccaca tacagacgat caaccaggcc tttatcgaca aggttattta
     4441 gcttcagggt gataccactg ggcagccctt gctgcgcgtt ggcgatttcg cggtctacca
     4501 tttcatataa cagacggcga gagttttgcg gtgacaccat taaataatca aatgtcaccg
     4561 ggcggtatgg gttttcaata aagttaaata cccgccgtac ttcgttggtg atacgcgcat
     4621 ctgcggtcag caacgaatag tcagtataaa gacgcgcggt tttttcgtta aagttcccgg
     4681 tcccgatgtg tgcgtaacgg accacttcac cgttttcttt acgtgaaatc aggaacagtt
     4741 tggcgtgaat tttcagcccc ggcgcagaga agataacgtg cacgcctgct tcggtcaggc
     4801 gcttcgccca gtgaatgttg gcttcttcgt cgaaacgcgc ctgtaactca accaccacgg
     4861 tcactttctt gccgttgtgc gcagcgtgga tcatcgagtc gatgatacgt gaatcttttg
     4921 ccacacggta aatgttaatt ttaatcgcca gtacgctcgg gtcgaacgaa gcctgacgca
     4981 gcagttccag cacatgctca aaggtgtgat aaggataata gagcaacaca tcgcgttcgc
     5041 gaatggcatc aaaaccattg cggaactggg ctttatcaaa ccaaatatgg cgtaaacgcg
     5101 gcagtggttt gttcaccaga ttggctttgc cgacattggg gaaattaata aagtctttaa
     5161 aattatgata acgaccgccg gggacgatgg agtcgtagcg ggaaatagtc agtttttcgc
     5221 gtaacacttc aaccagcgca ttgggcatat cgcgctgata aacaaaacgc accggctcag
     5281 cagttaaacg ctgcttgaga ctggaagaca tcaactccat caggctggct tccatctcat
     5341 gcactaaatc gtattcggca tcgcgggtca tcttcattga ataggcattc agcgcgtcat
     5401 aatcaaagaa gcctttgaaa atatcatcaa ggcagtaacg cagaatgtta tccagaagaa
     5461 tcatcggctt gcgtcgacgc ggtgcttctg gcggtaaatt cacaaagcgc ggcactttat
     5521 ctgatgggat ctccagcagc gcgtaacgga tggtatcgcc acggataatt tccaccgcca
     5581 gataggtgta atcatctttc aggaactgca ctaagtcagt gtcaggattg attaaaatcg
     5641 gcgtaatgtg ctgacgcaga tactgcttaa aataatgacg cagccagttt tgttgattga
     5701 cggagagctg gcgttcatta atcaggaaga tctggttacg cgccatctcc agcagcagct
     5761 cgttgtagag gccgtcgaat tcctgatcgg ctttcagcac ccgggactga attttgccca
     5821 gtaaatggcg ggaatgagag ttggagcctt gttcttcgct aataatgatg cgtcgcttca
     5881 gttcagcgaa gcggacttta tagaactcat caaggttatt ggaatagatc cccaggaaac
     5941 gcatcctttc aatcagcggg ttagatttgt ccgccgcttc ctgaagcacg cgttcattga
     6001 acgataacca actgagctct ttttcgatgt atagcttttc ctgacccatt accactttta
     6061 ctccgtttta ttcacgggac actgcctgga tattatggcg agcaatattg gcagatgtcc
     6121 aactatgcca gaaaaggggg aaaaaaactg ctggagcttg cgatttacag gtgaattgaa
     6181 aaacgccagc ggcagagctg gcgctttaac tacggggcat tactcgtcgg cagcgtagcc
     6241 ctgcggcggc agacgttgac catccagcca cgcggcgttt tcgtgcattt tcagacgacc
     6301 atcggcaaac cagctaatca ccagtggata aatagcgtgt tcctgggttt gcacgcgggc
     6361 ggtgatgtca tcttccgtat caccagcaaa taccgggact ttcgcctgta aaataaccgg
     6421 gccaccgtcc agttcatcgg tgacgaaatg caccgatgta ccgtgctctt catcaccatt
     6481 ttccagcgcc tgacgatggg tgtgtaatcc gggatatttc ggcagcagag aagggtgaat
     6541 gttcagcaaa cgcccggcat agtgggagac aaacgccggg ctgagaatgc gcataaaacc
     6601 agccagcacg accacatcgg gtgcgtacat gtcgatttca tgaatcaact cccggtcata
     6661 ggcttcacga ctgtcaaacg cgctggcgat gagcgtatgc gttgcaatac ccgcctggcg
     6721 ggcgcgttca aggccgaacg cgtcggcctt attgctgaaa actgcccgta tggtgccttt
     6781 aattttgttg gttttacagg cgtcaataat tgcctgtaaa ttacttccgt tgccggaaat
     6841 aagcaccaca atattcatta ttcgataacc acgcgttgtt cggaatcaga ggctttgatg
     6901 ataccgattt tccacgcgtt ttcaccgttg gcatttagca gggcgagggc tttgtccact
     6961 tccggagcag gcagggcgat aatcatcccg acgccgcagt tgaaggtgcg atacatttca
     7021 tggcgctcaa cgttaccagc cgtttgcagc cagttgaaca cttccggcca ctgccaggaa
     7081 gattcatcaa tcactgcctg agtattatct ggcaatacgc gcggaatgtt ttcccagaag
     7141 ccgccgccgg tcaggtgcgc aatggcatgc acatcgacct tttcaatcaa ctccagcact
     7201 gacttcacgt aaatgcgggt cggtgccagc agatgatcgg ctaatggctt accatcaagt
     7261 tcggtggttt gcggatcaca accgctgact tcaagaattt tgcgcaccag cgaatagccg
     7321 ttcgagtgcg gaccgctgga accgagtgca atcagcacat cgccgtcgct gactttagag
     7381 ccgtcgatga tttctgattt ttctaccacg ccaacgcaga aacccgcgac atcgtaatcc
     7441 tcaccgtgat acatccccgg catttctgcc gtttcgccac ccaccagtga acagcccgat
     7501 tgcagacatc cttccgcgat accgctgatc accgctgaag cggtatcaac atccagtttt
     7561 ccggttgcgt aatagtcgag gaaaaacagc ggttctgcac cttgcaccac caggtcatta
     7621 acgcacatgg cgaccagatc aataccaatg gtgtcgtgac gttttaagtc cattgccaga
     7681 cgcagcttgg tacctacgcc gtcagtgccg gaaaccagca cgggttcacg atatttttgc
     7741 ggcaatgcac acagcgcacc gaagccgccc agaccgccca tcacttccgg acgacgcgtt
     7801 ttcttcacta cgcctttgat tcttccaacc agagcattac ccgcgtcaat atcaacaccg
     7861 gcatctttgt agctaagaga ggttttatcg gtcactgctt gggtccccac gcgttacttg
     7921 cggtagaaaa ataaaattcg gcgcaattct aacagggaaa gcaaacgttt tcgagactgc
     7981 tttgcacaac ctttttgcac gtcttttccc cagacgcgcg gcgaaagaag acttgtgcca
     8041 gggcaaaggt tagttttcgg atggaata
//
LOCUS       DAZOZD010000187         7997 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOZD010000187 DAZOZD010000000
VERSION     DAZOZD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7997
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.187"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            44..568
                     /gene="radC"
                     /locus_tag="ACHUPB_003344"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="ACHUPB_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0760079.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUPB_003345"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="ACHUPB_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0760080.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUPB_003346"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHUPB_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0760081.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUPB_003347"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="ACHUPB_003347"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0760082.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUPB_003348"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHUPB_003348"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0760083.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUPB_003349"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHUPB_003349"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0760084.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUPB_003350"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHUPB_003350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0760085.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="ACHUPB_003351"
     CDS             5387..6511
                     /locus_tag="ACHUPB_003351"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0760086.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUPB_003352"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHUPB_003352"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0760087.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..>7997
                     /locus_tag="ACHUPB_003353"
     CDS             7317..>7997
                     /locus_tag="ACHUPB_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0760088.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLI"
BASE COUNT         2039 a         1849 c         2039 g         2070 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatc
//
LOCUS       DAZOZD010000188         7968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOZD010000188 DAZOZD010000000
VERSION     DAZOZD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.188"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUPB_003354"
     CDS             <1..239
                     /locus_tag="ACHUPB_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0760089.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="ACHUPB_003355"
     CDS             279..1142
                     /locus_tag="ACHUPB_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0760090.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUPB_003356"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHUPB_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR0760091.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="ACHUPB_003357"
     CDS             2679..4037
                     /locus_tag="ACHUPB_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0760092.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="ACHUPB_003358"
     CDS             4180..5130
                     /locus_tag="ACHUPB_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR0760093.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="ACHUPB_003359"
     CDS             5140..6522
                     /locus_tag="ACHUPB_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0760094.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6738..6812
                     /locus_tag="ACHUPB_003360"
                     /pseudo
     CDS             6738..6812
                     /locus_tag="ACHUPB_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUPB_003361"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="ACHUPB_003361"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR0760095.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAZOZD010000189         7959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOZD010000189 DAZOZD010000000
VERSION     DAZOZD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.189"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            166..320
                     /locus_tag="ACHUPB_003362"
                     /pseudo
     CDS             166..320
                     /locus_tag="ACHUPB_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            366..782
                     /gene="hicB"
                     /locus_tag="ACHUPB_003363"
     CDS             366..782
                     /gene="hicB"
                     /locus_tag="ACHUPB_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR0760096.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            861..2267
                     /locus_tag="ACHUPB_003364"
     CDS             861..2267
                     /locus_tag="ACHUPB_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR0760097.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2512..3657
                     /gene="ydcS"
                     /locus_tag="ACHUPB_003365"
     CDS             2512..3657
                     /gene="ydcS"
                     /locus_tag="ACHUPB_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR0760098.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3675..4688
                     /locus_tag="ACHUPB_003366"
     CDS             3675..4688
                     /locus_tag="ACHUPB_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0760099.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4689..5589
                     /locus_tag="ACHUPB_003367"
                     /pseudo
     CDS             4689..5589
                     /locus_tag="ACHUPB_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5579..6373
                     /locus_tag="ACHUPB_003368"
     CDS             5579..6373
                     /locus_tag="ACHUPB_003368"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0760100.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6395..7819
                     /gene="patD"
                     /locus_tag="ACHUPB_003369"
     CDS             6395..7819
                     /gene="patD"
                     /locus_tag="ACHUPB_003369"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0760101.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         1849 a         1889 c         2196 g         2025 t
ORIGIN      
        1 ttattcagtt ttgcaaatta gcgcaaagaa attctggaat cttccttcct gattttgcat
       61 tgcattctgc cgttgcggcg atttagtgct attttcgagc aaattacaca taggggtaaa
      121 cactaatgtt tactttattg atatactcag cggcagggag gcgatgtgaa acaaagcgag
      181 ttcagacgtt ggctcgaatc tcagggcgtc gatgtagcga atggccgcaa ccatttgaaa
      241 ctcaggtttc atgggaggcg cagtgtcatg ccgcgtcact cctgcgtaaa gcaatcctga
      301 aacaactcgg tttgagttaa tcgccaatta aaaaggttaa tgacatgcga gagacagtcg
      361 aaattatgcg ttatcccgtc actcttacac ccgcgccgga aggcggttat atggtttctt
      421 ttgtggatat ccctgaagcg ttgacgcagg gcgaaactgt cgctgaagcg atggaagcgg
      481 caaaagatgc tttactgacc gcatttgatt tttattttga agataacgag cttatcccgt
      541 taccttcgcc attaaatagt catgatcact ttattgaagt acctttgagc gtcgcctcta
      601 aagtattgct gttaaatgct tttttacagt cagaaatcac tcagcaagag ttagccaggc
      661 gaattggcaa acctaaacag gagattactc gcctatttaa cttgcaccat gcgacaaaaa
      721 tcgacgccgt ccagcttgcg gcaaaggcgc ttggcaaaga gttatcgctg gtgatggttt
      781 aattacagtt aacgaaaagt tgtcattttt aacaactgat atagactgcc gaatcatctg
      841 cacataatta cgattcgata atgaaaaaat accagcagct tgcagaacaa ttacgcgagc
      901 agattgcgtc gggtatctgg caacccggcg atcgtttgcc ttcgttgcgt gaccaggtgg
      961 cgctttcagg catgagcttt atgactgtca gccatgccta tcagttgctc gaaagtcagg
     1021 gatatattat cgcacgaccg cagtcgggtt attacgttgc gccacaggca ataaaaatgc
     1081 cgaaagcgcc agtcattcca gtcactcgag atgaagcagt cgatatcaac acttatattt
     1141 ttgatatgtt gcaggccagt cgcgaaccgt cggtcgttcc gtttgcctcg gcctttcccg
     1201 acccgcgact tttccccctc caacaactaa accgctcgct ggcgcaggta agcaaaaccg
     1261 ccacagcgat gagcgtgatt gaaaacttac cgccaggaaa cgcagaactg cgtcaggcta
     1321 ttgctcgtcg ctatgcctta cagggcatca ccatttctcc tgatgaaatt gtcattactg
     1381 ccggggcgtt agaggcatta aacctcagtt tgcaagcggt aactgaaccg ggcgattggg
     1441 tgatagtaga gaatccttgt ttctacggtg cgttgcaggc gctggagcgg ctacggctga
     1501 aggcgttatc ggtggcgacg gatgttaaag aagggatcga tcttcaggcg ctggaactgg
     1561 cgttgcagga ttatccggtg aaagcgtgct ggctgatgac taatagccag aacccactcg
     1621 gatttacctt aacgccgcaa aaaaaagcac aactggtggc gttactcaat cagtacaacg
     1681 taacgctgat tgaagatgac gtttacagcg aactttattt tggacgggaa aaaccgctgc
     1741 ctgcgaaagc gtgggatcgc cacgatggcg ttttgcattg ctcttcgttt tcgaaatgtc
     1801 tggtgcctgg ttttcgtatt ggttgggtcg ccgccggaaa acatgcacgt aaaattcaac
     1861 gcttgcagtt gatgagtacg ctttccacca gctcaccgat gcaacttgcg ctggtggatt
     1921 acctttccac gcgccgatac gacgcccatc ttcgtcgcct gcgtcgccag cttgcggaac
     1981 gtaaacaacg tgcctggcag gcactgctgc gttatctgcc tgcggaagtt aaaattcatc
     2041 atagtgacag cggttatttt ctctggctgg agctccccga gccgttagat gccggcgaat
     2101 taagcctggc ggcactgacg catcatatca gtattgcgcc gggtaaaatg ttttctaccg
     2161 gtgaaaactg gtcacgtttt tttcgtttta ataccgcgtg gcagtgggga gaacgtgaag
     2221 aacaggcggt aaaacaatta ggcaaactta ttcaagaacg gctgtaatag cgtttaattt
     2281 aattcctctt agattgggta atatgaattt cgaatagcag tcatatttcc taaccccttg
     2341 tctatactcc agaagataac cttacagacg gcataatgcg cggtagctca caacctgatt
     2401 aaattttctc agggacgaag gtgtgcctgc aagccgccgt ctatggttaa acaaggagat
     2461 atttttacgg cacggcggct gaacaattaa ttacgacagg agtaagacct tatgagcaag
     2521 acatttgccc gcagcagcct gtgtgcgctc accatgacaa taatgaccgc tcacgccgcc
     2581 gaaccaccta ccaatttaga taaaccggaa gggcgactgg atattatcgc ctggccggga
     2641 tacatcgaac gcggacaaac tgataaacaa tacgactggg taacgcaatt tgaaaaagag
     2701 acaggctgcg cggtgaatgt gaaaaccgcc gcgacttccg atgaaatggt cagtctgatg
     2761 accaaagggg gttacgatct ggttacggca tccggcgatg cctcgctgcg tttgattatg
     2821 ggcaaacgcg tgcagccgat taataccgcc ttgattccca actggaaaac gctcgatccg
     2881 cgcgtggtta aaggcgactg gtttaatgtt ggcggcaaag tttacggcac accttaccaa
     2941 tgggggccga acctgctgat gtacaacact aaaaccttcc cgacgccgcc ggatagctgg
     3001 caagtggttt ttgttgagca aaatctgccg gacggcaaga gcaataaagg ccgcgttcag
     3061 gcttatgatg gccctatcta cattgcggac gctgcgttgt tcgttaaagc cactcagccg
     3121 cagttgggca tcagcgatcc gtatcaacta accgaagaac agtaccaggc ggtgctgaaa
     3181 gtgctgcgcg ctcaacacag tttgatccat cgctactggc atgacactac cgtgcaaatg
     3241 agcgatttca aaaacgaggg tgtggttgct tccagtgcct ggccctatca ggccaacgcc
     3301 ctgaaagccg aaggccagcc tgttgctacc gttttcccga aggagggtgt taccggttgg
     3361 gctgatacca ccatgctgca tagcgaagcg aaacatccgg tttgcgccta caaatggatg
     3421 aactggtcat taacgccaaa agtgcagggc gatgtggcgg cctggtttgg ctcgttaccg
     3481 gtagtgccgg aagggtgtaa agccagtccg ttattaggcg aaaaaggttg tgaaaccaac
     3541 ggttttaact atttcgacaa aatcgccttc tggaaaacgc ctatagcaga agggggcaag
     3601 tttgttccct acagtcgctg gacgcaggat tacattgcca ttatgggcgg tcgctaactt
     3661 cgctggggtg ctttatgacg tacgcagtgg agtttgacaa cgtctcgcgg ttgtacggtg
     3721 acgtgcgcgc agtagatggc gtcagtattg cgataaaaga tggtgagttc ttctctatgc
     3781 tggggccgtc cggctccggc aaaaccacct gcctgcgcct gattgctggc ttcgaacagc
     3841 tttccggcgg ggctatctct atctttggta aacccgccag caatctgcca ccgtgggagc
     3901 gggacgtgaa tactgtcttt caggactacg cgctatttcc gcatatgtcg attcttgaca
     3961 atgtcgccta tgggctgatg gtcaaaggcg tgaataaaaa gcagcggcac gcaatggcgc
     4021 aagaggcgct ggagaaagtg gcgttggggt ttgtacagca acgtaaaccg tcacaacttt
     4081 ctggtggtca gcgccagcgg gttgctatcg ccagagcatt ggtgaatgaa ccgcgcgtat
     4141 tgctgttgga tgaaccgctc ggcgcactgg atctcaaatt gcgtgagcag atgcagctgg
     4201 aactgaaaaa actgcaacag tctctcggta tcacttttat cttcgttacc cacgatcagg
     4261 gcgaagcgtt atcgatgtcc gatcgtgtgg cggttttcaa taatggacgc attgagcagg
     4321 tcgattcccc gcgcgatctc tatatgcgcc cgcgcacgcc gtttgttgcc gggttcgttg
     4381 gtacatcgaa tgtttttgat ggactgatgg cagagaaact ttgtggcatg acgggaagct
     4441 tcgccctgcg accggaacat atccgcctca acacccctgg tgaactgcag gccaatggca
     4501 cgatccaggc ggtgcaatat cagggcgcgg caactcgttt tgaactgaaa ttgaacggcg
     4561 gtgaaaaact gcttgtgagt caggccaata tgacaggcga agaactgcct gccacgctca
     4621 cgcccggaca acaggtgatg gtttcctggt cgcgtgatgt gatggtgccg ctggttgagg
     4681 agaggtgaat ggcgatgaat gtattgcaat caccttcacg tccaggtctg gggctgtttt
     4741 tactgctgct tggcccgcta atgtggtttg gcattgtcta tttcggctcg ctgctgacac
     4801 tgttatggca gggattttat acttttgacg atttcaccat gtcggtaacg ccggaactga
     4861 cgctggcgaa tatccgtgcg ctgtttaatc cggcgaatta cgacatcatt ctccgcacgc
     4921 tgactatggc tgtggcagtc actatcgcca gcgccattct ggcttttcca atggcgtggt
     4981 atatggcgcg ctataccagc gggaaaatga aagcgttttt atattgcggt aatgttgccg
     5041 atgtgggcga gctacattgt taaagcctat gcctggacgt tattgctggc aaaagatggc
     5101 gtggctcagt ggtttttaca acatcttggg ctggaaccac tgctgactgc gttccttaca
     5161 ttacctgcgg tgggcggaaa tacgctgtca acttccgggc tggggcgctt tctggtgttt
     5221 ctctatatct ggttgccgtt catgatcctg cccgttcagg cggcgcttga gcgtttgccg
     5281 ccgtcattgt tgcaggcgtc ggctgatctc ggcgcacgtc cacgacaaac ctttcgctat
     5341 gtggtgctgc cgctggcaat cccgggtatt gccgctggct ctatctttac cttctcactc
     5401 actctgggcg attttatcgt cccgcagctg gttggtcctc caggatattt tatcggcaat
     5461 atggtttatt cccagcaggg ggcgattggc aatatgccga tggcggcggc attcaccttg
     5521 gtgccgatta ttctcatcgc actgtacctg gcgttcgtga aacgtctggg agcgttcgat
     5581 gcactctgaa cgcgcaccgt ttttcctcaa actggcggcc tggggcggcg ttgttttcct
     5641 acattttccc atcctgataa tcgccgccta tgcgtttaac actgaagatg cggcgtttag
     5701 ttttccaccg cagggcctga cgctgcgctg gtttagcgtg gcagcacagc gtagtgatat
     5761 tcttgatgcc gtgacactgt cacttaaagt ggcggcgctg gcgacattaa ttgcgctggt
     5821 gttagggacg ctggcagctg ccgcgctgtg gcgacgagac tttttcggca aaaacgccat
     5881 ttcgctgtta ctgctgctgc ccattgcgct gccgggcagc gtcactggtc tggcgttatt
     5941 aaccgccttt aaaaccatca atctggagcc gggatttttc accatcgtgg tcggtcatgc
     6001 gactttttgt gtagttgtgg tgtttaacaa tgtcatcgcc cgttttcgcc gcacctcctg
     6061 gagtctggtt gaggcgtcaa tggatcttgg ggccaatggc tggcaaacct tccgctacgt
     6121 agtgttgccg aatctcagtt cggcgttact ggcaggagga atgctggcgt ttgccttgtc
     6181 gttcgatgaa atcatcgtta cgacctttac ggcaggtcat gaacgaacgt tactgttgtg
     6241 gttgctcaat cagcttgggc gaccgcgtga tgtaccggta actaacgtgg tggcactgct
     6301 ggttatgttg gtaacaacct tgccgatcct gggggcctgg tggctaaccc gcgaaggcga
     6361 caatggtcaa taaccactga tacaggaata tgctatgcaa cataagttac tgattaacgg
     6421 agaactggtt agcggcgaag gggaaaaaca gcctgtctat aatccggcaa cgggggacgt
     6481 tttactggaa attgccgagg catccgcaga gcaggtcgat gctgctgtgc gcgcggcaga
     6541 tgcagcattt gccgaatggg ggcaaaccac gccgaaagtg cgtgcggaat gtctgctgaa
     6601 actggctgat gttatcgaag aaaatggtca ggtttttgcc gaactggagt cccgtaattg
     6661 tggcaaaccg ctgcatagtg cgttcaatga tgaaatcccg gcgattgtcg atgtttttcg
     6721 ctttttcgcg ggtgcggcgc gctgtctgaa tggtctggcg gcaggtgaat atcttgaagg
     6781 tcatacttcg atgatccgtc gcgatccgtt gggggtcgtg gcttctatcg caccgtggaa
     6841 ttatccgctg atgatggccg cgtggaaact tgctccggcg ctggcggcag ggaactgcgt
     6901 agtgcttaaa ccatcagaaa ttaccccgct gaccgcgttg aagttggcag agctggcgaa
     6961 agatatcttc ccggcaggcg tgattaacat actgtttggc agaggcaaaa cggtgggtga
     7021 tccgctgacc ggtcatccca aagtgcggat ggtgtcgctg acgggctcta tcgccaccgg
     7081 cgagcacatc atcagccatg ccgcgtcgtc cattaagcgt actcatatgg aacttggtgg
     7141 caaagcgcca gtgattgttt ttgatgatgc ggatattgaa gcagtggtcg aaggtgtacg
     7201 tacatttggc tattacaatg ctggacagga ttgtactgcg gcttgtcgga tctacgcgca
     7261 aaaaggcatt tacgatacgc tggtggaaaa actgggtgct gcggtggcaa cgttaaaatc
     7321 tggtgcgccg gatgacgagt ctacggagct tggaccttta agctcgctgg cgcatctcga
     7381 acgcgtcagc aaggcagtag aagaggcgaa agcgacaggg cacatcaaag tgatcactgg
     7441 cggtgaaaag cgcaagggta atggctatta ctatgcgccg acgctgctgg ctggcgcatt
     7501 acaggacgat gccatcgtgc aaaaagaggt atttggtcca gtagtgagtg ttacgccctt
     7561 cgacaacgaa gaacaggtgg tgaactgggc gaatgacagc cagtacggac ttgcatcttc
     7621 ggtatggacg aaagatgtgg gcagggcgca tcgcgtcagc gcacggctgc aatatggttg
     7681 tacctgggtc aatacccatt tcatgctggt aagtgaaatg ccgcacggtg ggcagaaact
     7741 ttctggttac ggcaaggata tgtcacttta tgggctggag gattacaccg tcgtccgcca
     7801 cgtcatggtt aaacattaag ttagatgtta gtggctgtta taggacggcc actatcgttt
     7861 taggttctga gtttaatggc ggttaagtat tagaatgatt acattaataa ctagcgtaaa
     7921 aattaggcta aatgtttgat ggcgattttt catcctgtc
//
LOCUS       DAZOZD010000190         7945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOZD010000190 DAZOZD010000000
VERSION     DAZOZD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.190"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..359
                     /locus_tag="ACHUPB_003370"
     CDS             <1..359
                     /locus_tag="ACHUPB_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR0760102.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHUPB_003371"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHUPB_003371"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0760103.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHUPB_003372"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="ACHUPB_003372"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0760104.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="ACHUPB_003373"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="ACHUPB_003373"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0760105.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="ACHUPB_003374"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="ACHUPB_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0760106.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHUPB_003375"
     CDS             complement(5490..6926)
                     /gene="selO"
                     /locus_tag="ACHUPB_003375"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0760107.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHUPB_003376"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="ACHUPB_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0760108.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2030 a         2086 c         1888 g         1941 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgac
//
LOCUS       DAZOZD010000191         7916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOZD010000191 DAZOZD010000000
VERSION     DAZOZD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.191"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(98..361)
                     /gene="cedA"
                     /locus_tag="ACHUPB_003377"
     CDS             complement(98..361)
                     /gene="cedA"
                     /locus_tag="ACHUPB_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0760109.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            544..2805
                     /gene="katE"
                     /locus_tag="ACHUPB_003378"
     CDS             544..2805
                     /gene="katE"
                     /locus_tag="ACHUPB_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0760110.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3063..3821)
                     /gene="chbG"
                     /locus_tag="ACHUPB_003379"
     CDS             complement(3063..3821)
                     /gene="chbG"
                     /locus_tag="ACHUPB_003379"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0760111.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3834..5186)
                     /gene="celF"
                     /locus_tag="ACHUPB_003380"
                     /gene_synonym="chbF"
     CDS             complement(3834..5186)
                     /gene="celF"
                     /locus_tag="ACHUPB_003380"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0760112.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5291..6133)
                     /gene="chbR"
                     /locus_tag="ACHUPB_003381"
     CDS             complement(5291..6133)
                     /gene="chbR"
                     /locus_tag="ACHUPB_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR0760113.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6141..6491)
                     /gene="chbA"
                     /locus_tag="ACHUPB_003382"
     CDS             complement(6141..6491)
                     /gene="chbA"
                     /locus_tag="ACHUPB_003382"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0760114.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6542..7900)
                     /gene="chbC"
                     /locus_tag="ACHUPB_003383"
     CDS             complement(6542..7900)
                     /gene="chbC"
                     /locus_tag="ACHUPB_003383"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR0760115.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
BASE COUNT         2119 a         2075 c         1776 g         1946 t
ORIGIN      
        1 ggcagctttt tgttacaggt ggtttgaatg aatgtagcaa cgaaatgcag aatttcaggt
       61 catgtaactc ccggcaaaac cgggaagtat gtaatcctta ctcagtcact tccccttcct
      121 ggcggatctg atttgcccaa cgttgggcag attcaggcac agtaaacgcc ggtgagcgca
      181 gaaatgactc tcccatcagt acaaacgcaa catatttgcc acgcagcatc cagacatcac
      241 gaaacgaatc catctttatc gcatgttctg gcggcgcggg ttccgtgcgt gggacatagc
      301 taataatctg gcggttttgc tggcggagcg gtttcttcat tactggcttc actaaacgca
      361 tattaaaaat tagaaaaact gtagtttagc cgatttagcc cctgtacgtc ccgctttgcg
      421 tgtatttcat aacaccgttt ccagaatagt ctccgaagcg ggatctggct ggtggtctat
      481 agttagagag ttttttgacc aaaacagcgg ccctttcagt aataaattaa ggagacgagt
      541 tcaatgtcgc aacataacga aaagaaccta catcagcacc agtcaccact acacgattcc
      601 agcgaagcga aaccggggat ggactcactg gcacctgagg acggatctca tcgtccagcg
      661 gctgaaccaa caccgccagg tgcacaacct accgcccctg ggagcctgaa agcccctgat
      721 acacgtaacg aaaaacttaa ttctctggaa gacgtacgca aaggcagtga aaattatgcg
      781 ctgaccacta atcagggcgt gcgcatcgcc gatgatcaaa actcactgcg tgccggtaac
      841 cgtggtccaa cgctgctgga agattttatt ctgcgcgaga aaatcaccca ctttgaccat
      901 gagcgcatcc cggaacgtat tgttcatgca cgcggatcag cagctcacgg ttatttccag
      961 ccatataaaa gcttaagcga tatcaccaaa gcggatttcc tctcagatcc gaacaaaatc
     1021 accccagtat ttgttaggtt ctctaccgtt cagggtggcg ctggctctgc agataccgtg
     1081 cgtgatatcc gtggttttgc caccaagttc tatactgaag agggtatttt tgacctcgtt
     1141 ggcaataaca cgccaatctt ctttatccag gatgcgcata aattccccga ttttgttcat
     1201 gcggtaaaac cagaaccgca ctgggcaatt ccacaagggc aaagtgccca cgatactttc
     1261 tgggattatg tttctctgca acctgaaact ctgcacaacg tgatgtgggc gatgtcggat
     1321 cgcggcatcc cccgcagtta ccgcaccatg gaaggcttcg gtattcacac cttccgcctg
     1381 attaatgccg aagggaaggc aacgtttgta cgtttccact ggaaaccact ggcaggtaaa
     1441 gcctcactcg tttgggatga agcacaaaaa ctcaccggac gtgacccgga cttccaccgc
     1501 cgcgagttgt gggaagccat tgaagcaggc gattttccgg aatacgaact gggcttccag
     1561 ttgattcctg aagaagatga attcaagttc gacttcgatc ttctcgatcc aaccaaactg
     1621 atcccggaag aactggtgcc cgttcagcgt gtcggcaaaa tggtgctcaa tcgcaatccg
     1681 gataacttct ttgccgaaaa cgaacaagca gcattccatc caggtcatat tgttcctggt
     1741 ctggacttca ccaacgatcc gctgttgcag ggacgtttgt tctcctatac cgatacacaa
     1801 atcagtcgtc ttggtgggcc gaatttccat gagattccga ttaaccgtcc gacctgccct
     1861 taccacaact tccagcgtga tggcatgcat cgtatgggga tcgacactaa cccggcgaat
     1921 tacgaaccga actcgatcaa cgataactgg ccgcgcgaaa caccgccggg accgaaacgc
     1981 ggcggttttg aatcatatca ggaacgcgtg gaaggaaata aagttcgtga gcgcagtcca
     2041 tcgtttggcg aatattattc ccatccgcgt ctgttctggc taagtcagac gccattcgag
     2101 cagcgccata ttgtcgatgg tttcagtttt gagttaagca aagtcgttcg tccgtatatt
     2161 cgtgagcgcg ttgttgacca gctggcgcat attgatctca ctctggccca ggcggtggcg
     2221 aaaaatctcg gtatcgaact gactgacgac cagctgaata tcaccccacc tccggacgtc
     2281 aacggtctga aaaaggatcc atccttaagt ttgtacgcca ttcctgacgg tgatgtgaaa
     2341 ggtcgcgtgg tagcgatttt acttaatgat gaagtgagat cggcagacct tctggccatt
     2401 ctcaaggccc tgaaggccaa aggcgttcat gccaaactgc tctactcccg aatgggtgaa
     2461 gtgactgcgg atgacggtac ggtgttgcct atagccgcta cctttgccgg tgcaccttcg
     2521 ctgacggtcg atgcggtcat tgtcccttgc ggcaatatcg cggatatcgc tgacaacggc
     2581 gatgccaact actacctgat ggaagcctac aaacacctta aaccgattgc gctggcggga
     2641 gacgcgcgca agtttaaagc aacaatcaag gtcgctgacc agggtgaaga agggattgtg
     2701 gaagctgaca gcgctgacgg tagttttatg gatgaactgc taacgctgat ggcagcacac
     2761 cgcgtgtggt cacgcattcc taagattgac aaaattcctg cctgatggga ggcggcgcaa
     2821 ttgcgccgcc tcaaatgatt tacatagtgc gctttgttta tgccggatgc ggcgtgaacg
     2881 ccttatccgg cctacaaaac tgtgcaaatt caatatattg caggaaacac gtaggcctga
     2941 taagcgaagc gcatcaggca gttttgcgtt tgtcagcagt ctcaagcggc gcagttacgc
     3001 cgcctttgta ggaattaatc gccggatgca aggttcacgc cgcatctggc aaacatcctc
     3061 acttacacat cccgataact ccccaaccga taaccacgct gagcgatagc acctttcaac
     3121 gacgctgatg tcaacacatc cagctccgtt aagcgtggga aacagtaagc actctgacgg
     3181 atagtattat cgataaacgc cggatgacac atcacctcca gcgaacgatc accccgatgg
     3241 cttgcgtcat ccagcacttg caggaacagt gcctcgctaa tctcttcacc ataaaacgcg
     3301 ctactgaatc cctgagaact gcgcagatta gccggtaaat caccagagtt agatagcggc
     3361 tgacggtcga tgcgcaacgc aatcccctct tcagccgcaa acctggcaac aatcgggaaa
     3421 atctgcggga acatatgcac atgatgatgg ctatcaagat gcgtaggttt gcgtccaaat
     3481 agctcaatga aacgcaaata ctgactggca agctcctgag taatttcttc cagcggtaaa
     3541 gcatcttctt ctgccaactg ccagatccat tttcccagca caccatcgcg ggttaacccc
     3601 ggcatagctg tcagtggctt gcccatagta aggacaaagt acatccctat ggccagactc
     3661 ggttcatcac gactcaactg caccgcatgg tcaatagcct gcccattcac cagcgccgtc
     3721 gtcgatgtga caatcccatt gcgacaggcc tcgataatgc cgtagttctg acctttgctt
     3781 aagccaaaat catcggcatt aacaatcagt aagcgttcca taatcagcct cggttaatgt
     3841 gcttttttaa gctctgcgat gcagtcggca aaatttggca gccatttctc gtgcgccaga
     3901 atcatctcgc gtgccagcag ctcagcatcg cgatcggaat gcaccaacgg actaaggttt
     3961 agcgccagta acacatcgtt aaattctccg ctaagtgcgg cgttgctggc agcaatctcg
     4021 aagcctttaa tggtgtgaat cagccccatc actttatcat cgaaatgcgt aatgcgcgga
     4081 tgtggcgtcg cgccatcgcg ccccagctta caggtcattt ctaccgccca gtctgccgga
     4141 atattatcaa tatgcccatg atgcgggata ttaacgtaat gttcagcttg cttgtcgttg
     4201 tagatagcgt tgatcacttc gcacgctgca tcagagtaat aagccccacc gcgctgttcc
     4261 agttctttcg gcttaacttt caactccggg tttttataca gctcaaaaag ttgtttctcg
     4321 actttctgta ctacctgcgc tcgtgcgccg cctttgtagt attcgcccat ttcaatagcc
     4381 agcatctctt tttgcttgaa gtaataaagc aaataagaac acggcaacag attcagagaa
     4441 cgaattaagc cttcgctaaa tggcagatcg aaaatatttt taacgccaga tgcttttaac
     4501 tgcccggatg ccacaccatc aagcaattcg gcaaagcgcg acttgccatt taccagcaca
     4561 tccttaatga acaccatatg gttgaggccg aacaaatcga tagataaatc atcgctgtct
     4621 ttcagcatca gaacatcgtg aataaacatc ttcatgccga tcggaatatt acacacgccg
     4681 ataaagcgtt taaatccggt atgacgataa acggcttcag taaccattcc cgccgggtta
     4741 gtgaagttaa tcacccatgc attcggacaa agttcttcga catcttttac gatgtcaaaa
     4801 atcaccggaa tggtacgcag acctttaaac agaccgcccg cgccgttggt ttcctggcca
     4861 agataaccat gacttaatgg aatacgttca tccagttcgc gcgccggtaa ttggcctacg
     4921 cgcagttggg tagtaacgaa atcagcatct ttcaatgctt cgcggcgatc cagcgtttta
     4981 taaagcttca tcgggacgcc agcgttatca atcatccgtt ggcagagttc aaaaatgata
     5041 tccagtttct ctttaccatc ttcgacatcc accagccata attcgctgac cggcaattcg
     5101 tgataacgct taataaatcc ttccagtaac tccggggtat agctgctccc gccaccaata
     5161 gtgacgactt ttaatttctg gctcataatt tctcccttca gtacagaata ctgatatctg
     5221 gcatatctgc ccccccggac ataaataatc cagcaacagg acagatatgt gaattgtcag
     5281 gtataacgac ttactgatta aattcagtca atttcttacg atagctctta ggcgtaaagg
     5341 acgttaattt cttaaacgtt ttaataaaca aactcgggct actataaccg gcctcaaacg
     5401 caatatccgt cacggaatag ttggtcattt ccagttgttt tttggcaaaa ttaatacgga
     5461 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
     5521 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
     5581 ctttatcatg catcttttct accgtacttt tcagccactg cggtacatca tcaatcacct
     5641 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
     5701 cttccagccc tgtttcgcgg aaattcaatg aagaaatcac tgtttccaca taggtgagaa
     5761 aagcgttatt ggtccggtat acctgcgaag cgacaaagca ataaggcaac aacggcaggt
     5821 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatacgcgtg gcaccaaact
     5881 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
     5941 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
     6001 aatagtcgtg ctgatgcagc ccgctgatac tctcagtttt gttatagata aacacatgga
     6061 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
     6121 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
     6181 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
     6241 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
     6301 gcttcattca atgccatccg tgactgatcc atcatggctt ttgctgcagc aaaatcgccc
     6361 tgcttcgcct gtttcagtgc cgcatacgcc aggctgcgcg cttgtccgga gttgatgata
     6421 agccccatca ccacttcttc cagctcttca gcttccgttt gcgtatcggg aatgttatcg
     6481 agatccatca tacatcgttc ctctctattc ttaccggcac gattacccgt accggcatcg
     6541 attaaaattt cagggcgtta gcgatatctt cttcgcgctc ttctttatca atcgcgtttt
     6601 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgata ccaaggttga
     6661 agagtgcaac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     6721 ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg
     6781 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     6841 tcatgataat tggcagacca aacagaatcg gttcgttaat ctggaagatg ccggacggca
     6901 gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga
     6961 tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg
     7021 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     7081 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     7141 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     7201 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     7261 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     7321 agccgggaat taatgccgag aaggaacgcg atactgaagc aggtacgcta tcgggcaatt
     7381 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     7441 taatcccgga gatgatattc gctccaccta accagtttgc gccaaccaca taggcctcac
     7501 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca gccgccagcg
     7561 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     7621 ttatccctaa tgttccgtta tatacgttgc cgccaatacc tttcaggcca ttaagtgttt
     7681 caatggttga ggcgtcgagg cgaataccta aggaataaaa aaacgccccc tccccaaagc
     7741 ttagaaaaac gttgttaatt aatacaaaca tggcccccgc aagggttaac ggcattaagc
     7801 gaataaagcc atttttgatt gcattaacgt gtggctgctt tcctatttta actgcaaaag
     7861 ggaggagtac cttttcaagc gatgcaataa cattactcat agaaaaatac ccttaa
//
LOCUS       DAZOZD010000192         7818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZOZD010000192 DAZOZD010000000
VERSION     DAZOZD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.192"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="ACHUPB_003384"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="ACHUPB_003384"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR0760116.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="ACHUPB_003385"
     CDS             complement(2362..2802)
                     /locus_tag="ACHUPB_003385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0760117.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUPB_003386"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHUPB_003386"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0760118.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="ACHUPB_003387"
     CDS             3520..4218
                     /locus_tag="ACHUPB_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0760119.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="ACHUPB_003388"
     CDS             4447..5655
                     /locus_tag="ACHUPB_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0760120.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUPB_003389"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="ACHUPB_003389"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0760121.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
BASE COUNT         1790 a         1918 c         2076 g         2034 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttc
//
LOCUS       DAZOZD010000193         7812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOZD010000193 DAZOZD010000000
VERSION     DAZOZD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.193"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(392..481)
                     /locus_tag="ACHUPB_003390"
     tRNA            complement(392..481)
                     /locus_tag="ACHUPB_003390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Ser,seq:cga)
     gene            575..1372
                     /gene="mtfA"
                     /locus_tag="ACHUPB_003391"
     CDS             575..1372
                     /gene="mtfA"
                     /locus_tag="ACHUPB_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0760122.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1473..1548
                     /locus_tag="ACHUPB_003392"
     tRNA            1473..1548
                     /locus_tag="ACHUPB_003392"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1506..1508,aa:Asn,seq:gtt)
     gene            1862..7812
                     /gene="yeeJ"
                     /locus_tag="ACHUPB_003393"
                     /pseudo
     CDS             1862..7812
                     /gene="yeeJ"
                     /locus_tag="ACHUPB_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         2187 a         1921 c         1893 g         1811 t
ORIGIN      
        1 gttagtgtga tacaaaggct ttcaaaaaag ctgggaaatt caacaactct tggaaattat
       61 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttgactc aattattgac
      121 cccttctcta cgcaacttca gttcccacca ccaactttgc ggcagtttca taggatgaat
      181 gtctaaaaga ataatggtga gcgataagaa aacgactgaa taactgcaca ttttcgctcg
      241 aaagcttcct gtcagatcca tagcgaatca agtgctgaat gtcacagtat cgaacagaaa
      301 acagtgacga tctaaccctt caagaatatt ctacaattgt tctgtttagg aaaagcaagg
      361 cgggaagtca ggagataagt cattgataaa gtggcggaga gagggggatt tgaacccccg
      421 gtagagttgc ccctactccg gttttcgaga ccggtccgtt cagccgctcc ggcatctctc
      481 cgttcagatg gttgccatga tgccaggaaa tttggcattt taacagtccc tgttcgtgca
      541 attttgttca agtgacgagt ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca
      601 agaatcagca catcaaactg cccttccctg gcaggaagca ctatcgatcc cccttttaac
      661 gggtctgacg gaacaggaac aaagcaaatt agtcactctt gccgaacgtt ttttacagca
      721 aaagcggctt gttcctttac agggctttga actggattca ttaagaagct gccggatagc
      781 acttctattt tgcctacccg ttctggagtt aggactggaa tggctggatg gttttcatga
      841 agtcttaatt tatcctgcgc catttgtggt cgatgatgaa tgggaagacg atatcggtct
      901 ggtgcataac caacgtattg ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt
      961 gaactggttg gatatacaag attcttttga tgcatctggt tttaacctga ttattcatga
     1021 agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc agcggagttc cctttattcc
     1081 gttgcgtgag gttgctggct gggaacacga tcttcatgct gcaatgaaca acattcagga
     1141 agaaatcgaa ttggttggcg agaatgcggc gagcattgat gcttatgcag ccagcgatcc
     1201 tgctgaatgt tttgccgtac tttctgaata tttctttagc gccccagaac tttttgctcc
     1261 tcgtttccct tcattgtggc aacgtttctg ccaattttat caacaagatc ctttgcagag
     1321 actgcatcac gctaatgata cagactcgtt ttcggcgacg aatgttcatt aattaacaac
     1381 tttgcagatt aattaaccaa ttgaaatgac ttatgaaatt tagtgttgac agacaaggta
     1441 ccgctaagta atatgtgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc
     1501 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa ttctaaaaat
     1561 tcgctttttt agcgcaatgt cactgacctt agttgaacat tgttttttaa cggatagcgg
     1621 gtttttaaca ccttaagcgc cctcgacctt tatggttgag ggcgttttgc tatgaacgcc
     1681 atcaccattt tcccctcgat tataaaactt gagttattca gtagtctccc ctcttgcaac
     1741 tcacacccaa aactgcctaa cgaaaagtta ttaattttca atcgtattgc tatcagtatt
     1801 tacatttttt cgctgtgcta gaaagggcgc atttatgtta gctcgttcag ggaaggtaag
     1861 catggctacg aagaagagaa gtggagaaga aataaatgac cgacaaatat tatgcgggat
     1921 gggaattaaa ctacgccgct taactgcggg tatctgtctg ataactcaac ttgcgttccc
     1981 tatggctgcg gcagcacaag gtgtggtaaa cgccgcaacc caacaaccag ttcctgcaca
     2041 aattgccatt gcaaatgcca atacggtgcc ctacaccctt ggagcgctgg aatcggccca
     2101 aagcgttgcc gaacgtttcg gtatttcggt ggctgagtta cgcaaactca accagtttcg
     2161 tacgtttgct cgaggttttg ataatgtccg ccagggtgat gaactggatg tcccggcaca
     2221 agttagtgaa aaaaaattaa ccccgccgcc gggtaatagc agtgacaacc tcgagcaaca
     2281 gatagccagt acttcacagc aaatcgggtc tctgctcgcc gaagatatga acagcgagca
     2341 agcggcaaat atggcgcgtg gatgggcctc ttctcaggct tcaggcgcaa tgacagactg
     2401 gttaagccgc ttcggtaccg caagaatcac gctgggcgtg gatgaagatt ttagcctgaa
     2461 gaactcccag ttcgattttc tccatccgtg gtatgaaacg cctgataatc tctttttcag
     2521 tcagcatact ctccatcgta ctgacgagcg tacgcagatt aacaacggct taggttggcg
     2581 tcatttcact cccacatgga tgtcgggcat caacttcttt ttcgaccacg atcttagccg
     2641 ttaccactcc cgcgtcggca ttggcgcgga gtactggcgc gactatctaa aattaagcag
     2701 taacggctat ttgcgactga ccaactggcg cagcgcacct gaactggaca acgattataa
     2761 agcacgcccg gccaatggct gggatgtacg cgcagaaagc tggctacccg cctggccgca
     2821 ccttggcggt aaactggtct atgaacagta ttatggcgat taagtggccc tgttcgataa
     2881 agacgatcgg caaagtaatc ctcatgccat aaccgctgga cttaactata cccccttccc
     2941 gctgatgacc ttcagcgcgg agcaacgcca gggtaaacag ggcgaaaatg acacccgttt
     3001 tgccgtcgat tttacctggc aacctggcag cgcaatgcag aaacagcttg acccgaatga
     3061 agtcgctgca cggcgtagcc ttgcaggcag ccgttatgat ctggtggatc gcaacaacaa
     3121 tatcgttctg gaatatcgca aaaaagaact ggttcgccta accctgacag accccgtgac
     3181 agggaagtca ggagaagtga aatcactggt ttcgtcgcta caaaccaaat atgccctgaa
     3241 aggctataac gtcgaagcca ccgcactgga agctgccggt ggcaaagtgg tcacaacggg
     3301 taaagatatt ctagttaccc tgccggctta ccggttcacc agtacgccag aaaccgataa
     3361 cacctggccg attgaagtca ccgccgaaga tgtcaaaggc aatttgtcga atcgtgaaca
     3421 gagcatggtg gtcgttcagg cacctacgct aagccagaaa gattcctcgg tatcgttaag
     3481 tacccaaaca ttgaacgcgg attcccattc aaccgccaca ctgactttta ttgcgcatga
     3541 tgcagcaggt aatcctgttg tcgggctggt gctctcgacg cgtcacgaag gtgttcagga
     3601 catcaccctt tctgactgga aagataatgg tgacggaagc tatacccaga tcctgaccac
     3661 aggagcgatg tctggcacgc tgacgctgat gccacagctg aatggtgtgg atgcggctaa
     3721 agcccccgcc gtggtgaata tcatttctgt ttcgtcatcc cgaactcact cgtcaattaa
     3781 gattgataag gaccgttatc tctccggcaa tcctatcgag gtgacggtag aactgagaga
     3841 tgaaaatgac aaacctgtta aggaacaaaa acagcaactg aataacgcag tcagcatcga
     3901 caacgtgaaa ccaggagtca ctacagactg gaaagaaacc gcagatggcg tctataaggc
     3961 gacctatacc gcctatacca aaggcagtgg acttactgcg aagctattgg aatgaagatt
     4021 tgcataccgc tggatttatc atcgacgcca acccgcagtc agcgaaaatt gcgacattat
     4081 ctgccagcaa taatggtgta ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg
     4141 tcgctgatga aggaagcaac ccaatcaatg atcataccgt cacgtttgcg gtattaagcg
     4201 gatcggcaac ttccttcaac aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga
     4261 cttttgatct gaaaagtagt aagcaggaag acaacacggt tgaagtcacc cttgaaaatg
     4321 gcgtgaaaca aacgttaatc gtcagttttg tcggcgactc gagtaccgcg caggttgatc
     4381 tgcagaagtc gaaaaatgaa gtggttgctg acggcaatga cagcgccaca atgaccgcga
     4441 ccgtccggga tgcaaaaggc aacctgctca atgacgtcat ggtcactttc aatgttaatt
     4501 cagcagaggc gaaactgagc caaaccgaag tgaatagcca cgacgggatc gccacagcta
     4561 cgctgaccag tttgaaaaat ggtgattata gggttacggc ctctgtgagc tctggttccc
     4621 aggctaatca acaggtgaat tttatcggtg atcaaagtac tgctgccctg accctcagtg
     4681 tgccttcagg tgatatcacc gtcaccaaca cagctccgca acatatgact gcaaccttgc
     4741 aggataaaaa tggcaaccca ctaaaagata aagaaatcac cttctctgtg ccaaacgacg
     4801 tcgcaagtaa gttctcgatt agcaacggag gaaaaggcat gacggatagt aacggggttg
     4861 caatcgcctc cctgaccggc acgttagcgg gcacgcatat gatcatggct cgtctggcta
     4921 acagcaatgt cagcgatgca cagccaatga cgtttgtggc ggataaagac agagcggttg
     4981 tcgttttgca aacatcgaaa gcggaaatca ttgggaatgg cgtggatgag acaactctga
     5041 cagcaacagt gaaagatccg tcgaatcatc cggtggcggg gataacggta aacttcacca
     5101 tgccacagga cgttgcggca aactttaccc ttgaaaataa cggtattgcc atcactcagg
     5161 ccaatgggga agcgcatgtc acgctgaaag gtaaaaaagc gggcacgcat acggttaccg
     5221 caacgctggg taataacaat accagtgatt cgcagccggt aacatttgtg gcggacaaag
     5281 cctcggctca ggttgtcctg cagatatcaa aagatgagat cacaggtaat ggcgtcgata
     5341 gcgcaacgct aactgcaacg gttaaagatc agttcgacaa tgaggtgaat aatcttccgg
     5401 taacattcag ctcagcctct tcaggactca ccctgacccc gggagtaagt aataccaatg
     5461 agtctggcat cgcgcaggcc actctcgcag gcgttgcctt tggtgagcag acggtcactg
     5521 catcactggc taataatggt gccagcgaca acaaaactgt gcattttatt ggcgacacag
     5581 cggcggcaaa aattatcgag ttgacgcctg tcccagacag cataatcgcc ggtaccccgc
     5641 agaacagctc cggcagcgcc atcaccgcca cagtcgttga taataatggc tttccggtga
     5701 aaggtgtgac tgtgaacttc accagcagaa caaactctgc cgaaatgacg aatggcggcc
     5761 aagccgtaac gaacgaacag ggtaaggcta ccgtcactta taccaatacc cgctcctcga
     5821 tagaatcagg agcgagaccg gataccgttg aggccagtct ggaaaatggt agctccacgc
     5881 ttagcacatc aattaatgtc aacgctgatg cgtctacggc acatctcacc tggctacagg
     5941 cactttttga tacagtctcc gcaggcgaga caaccagtct gtatattgag gtgaaggata
     6001 attacggcaa cggtgtcccc cagcatcagg tgaccctcag cgtttcacca agtgaaggcg
     6061 tgacccccag taataacgct atatatacta ccaaccacga cggcaatttt tacgcaagct
     6121 ttaccgctac aaaagccggg gtttatcaag tgacggcaac cctcgaaaat ggcgattcga
     6181 tgcaacaaac agtgacctat gtgccgaacg tcacgaatgc cgaaatcacg ctggcaacct
     6241 cgaaggatcc ggtgattgcc gacaataacg atctcacgac actaacagca acagtcgctg
     6301 atacagaggg caatgcgata gccaacactg aagtaacatt tactctgccg gaagatgtta
     6361 aggcgaactt cacgctgagc gatggcggta aagcgattac tgatgctgaa ggcaaagcga
     6421 aagtcacgct gaaaggtaca aaagcaggcg ctcatactgt tacagcatcg ataactggcg
     6481 gtaagagtga gcagttggtg gtgaacttta ctgcggatac gctcactgcg caggttaatc
     6541 ttaacgttac cgaggacaat tttatcgcta ataacgtcgg gatgaccagg ctgcaggcaa
     6601 cagtgactga tggaaacggc aacccgttag ccaatgaggc ggtgacattc acgctaccgg
     6661 cagatgtgag cgcaagcttt actctcggac aaggcggttc cgccattact gacatcaacg
     6721 gcaaggctga agttacactg agcggtacaa aatccggcac ctaccccgtg acagttagcg
     6781 tgaacaatta tggtgtcagt gatacgaaac aggtgacttt gattgccgat gctggtaccg
     6841 caacactagc ctccttaacc tctgtatact cattcgtcgt cagcacgacc gaaggcgcga
     6901 ccatgactgc aagcgtcact gacgctaacg gcaacccggt agaaggcata aaagttaatt
     6961 tccgcggaac ctccgtcacg ctaagcagca ccagcgttga aacggatgat cggggtttca
     7021 ctgaaattct tgtgacaagc accgaagtcg gactgaaaac agtttcagcc tctctggcag
     7081 ataaacctac tgaagtcata tcgcgattac tgaatgcaaa agcagatatt aattctgcaa
     7141 cgattaccag tctggagata cctgaaggtc aggtcatggt cgcacaagac gtagcagtta
     7201 aagctcacgt caacgaccag tttggcaacc cgattcttaa tgaatctgta acattcagtg
     7261 cagagccacc agagcacatg accatcagcc aaaatattgt ctctactgat acgcatggta
     7321 tagccgaggt ctccatgacg cccgaaagaa acggttcgta tatggtgaaa gcatccctgg
     7381 cgaatggagc ctcacttgag aaacaactgg aggctattga tgaaaaactg acactctcgg
     7441 cgtccagccc gcttatcggt gtcaattccc caacaggtgc aactctgacg gcaacgctaa
     7501 cttctgcaaa tggcactcca gtggagggtc aggtcatcaa ctttagcgta acgccagaag
     7561 gtgcgacgtt aagtggcgga aaagtgagaa ctaactcttc tggtcaggct ccagtcgttc
     7621 tgaccagcaa taaagtcggt acatatacgg tgactgcatc gttccataac ggcgtaacaa
     7681 tacagacaca gacaaccgtg aaagtcactg gcaactcaag caccgcccat gttactagct
     7741 ttatcgctga tccatcgact atcgccgcca ccaacagtga tttaagtacc ttaaaggcaa
     7801 cggttgagga tg
//
LOCUS       DAZOZD010000194         7776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOZD010000194 DAZOZD010000000
VERSION     DAZOZD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.194"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUPB_003394"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUPB_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR0760123.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUPB_003395"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUPB_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0760124.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUPB_003396"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUPB_003396"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0760125.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUPB_003397"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUPB_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0760126.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUPB_003398"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUPB_003398"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0760127.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUPB_003399"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUPB_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0760128.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUPB_003400"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHUPB_003400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0760129.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAZOZD010000195         7750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOZD010000195 DAZOZD010000000
VERSION     DAZOZD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.195"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            44..304
                     /gene="dinJ"
                     /locus_tag="ACHUPB_003401"
     CDS             44..304
                     /gene="dinJ"
                     /locus_tag="ACHUPB_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR0760130.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            307..585
                     /gene="yafQ"
                     /locus_tag="ACHUPB_003402"
     CDS             307..585
                     /gene="yafQ"
                     /locus_tag="ACHUPB_003402"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR0760131.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            741..1481
                     /gene="dpaA"
                     /locus_tag="ACHUPB_003403"
     CDS             741..1481
                     /gene="dpaA"
                     /locus_tag="ACHUPB_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0760132.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1452..2219)
                     /locus_tag="ACHUPB_003404"
     CDS             complement(1452..2219)
                     /locus_tag="ACHUPB_003404"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0760133.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2425..3003)
                     /gene="lpcA"
                     /locus_tag="ACHUPB_003405"
     CDS             complement(2425..3003)
                     /gene="lpcA"
                     /locus_tag="ACHUPB_003405"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0760134.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3243..5687
                     /gene="fadE"
                     /locus_tag="ACHUPB_003406"
     CDS             3243..5687
                     /gene="fadE"
                     /locus_tag="ACHUPB_003406"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0760135.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5730..6176)
                     /gene="ivy"
                     /locus_tag="ACHUPB_003407"
     CDS             complement(5730..6176)
                     /gene="ivy"
                     /locus_tag="ACHUPB_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0760136.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6357..7127
                     /gene="yafV"
                     /locus_tag="ACHUPB_003408"
     CDS             6357..7127
                     /gene="yafV"
                     /locus_tag="ACHUPB_003408"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0760137.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVAWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(7169..>7750)
                     /locus_tag="ACHUPB_003409"
     CDS             complement(7169..>7750)
                     /locus_tag="ACHUPB_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0760138.1"
                     /translation="KQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKS
                     HGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISS
                     ADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILT
                     NDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1836 a         1954 c         2032 g         1928 t
ORIGIN      
        1 tacaattcaa gctgaataaa tatacagcac aggagatccc ccaatggctg ctaacgcgtt
       61 tgttcgcgcc cgaatcgatg aagatctgaa gaatcaggcg gcggacgtac tggccgggat
      121 ggggctgacc atctctgacc tggttcgcat aaccctcaca aaggtcgcgc gtgaaaaggc
      181 attgccgttt gatttacgcg agcctaatca attaaccatt caatcaatca aaaacagcga
      241 agctggcgtt gatgttcata aggccaaaga cgctgatgat ttatttgata aattaggaat
      301 ttaaatatga ttcaaaggga tattgaatac tcgggacaat tttcaaagga tgtaaaactt
      361 gcacaaaagc gtcataagga tatgaataaa ttgaaatatc ttatgacgct tcttatcaat
      421 aatgctttac cgcttccagc tgtttataaa gaccacccgc tgcaaggttc atggaaaggt
      481 tatcgcgatg ctcatgtcga accggactgg atcctgattt acaaacttac cgataaactt
      541 ttacgatttg agagaactgg aactcacgcg gcgctctttg ggtaaagcac gctgccgatg
      601 aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc
      661 ttccaaatcc agactattcc gatttgggat ctattttgcc gtcctggcgt gtgtaaccgt
      721 tttatcaagg aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc
      781 cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag
      841 agtataagca gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta
      901 cgctcgatct ctacgtcaaa atgggcgagc aatatcagct gctcgacagt tataaaatct
      961 gtaaatattc cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag
     1021 ggttttatag cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta
     1081 atattggttt ccccaatgcc tatgaccgtg cgcatggtta tgaagggaaa tacctgatga
     1141 tccacggcga ttgtgtttcc atcggctgct acgcaatgac caatcagggt attgatgaga
     1201 tcttccagtt cgttactggt gcgctggtgt ttggtcagcc gagcgtgcaa gtgagtattt
     1261 acccgttccg catgaccgac gccaatatga agcgccataa atattccaac tttaaggact
     1321 tctgggagca actgaagccg ggttacgact actttgagca aacccgtaag ccaccgaccg
     1381 tttctgtggt caatggtcgt tacgtggtca gcaagccgtt aagccacgaa gttgtgcagc
     1441 cacaactggc atcaaactac acgctccccg aggcaaaata agcgccattc gcctggcata
     1501 atcttttgcc aggtttcatt gcccgtcagc ggctgtgttg caatcaccgt gaccacatca
     1561 ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct gatccagcag cgttgccacg
     1621 ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg agcaatacgc catcacatag
     1681 cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc gcagttcatc cgccagtgag
     1741 gcgatatatt taaacactgc cgccatgttg cccggtgtgc gcgggtaacg ctgcgttaat
     1801 ttatgcagga gccagcagaa ggctttttcg ctatcggttt caccgaccgg gcggaagttg
     1861 ccggtttcca gtgatttgta gcccgtcagt tgtccgttat gggcataagt ccagttacgc
     1921 ccccataact cacgggtaaa cgggtgagta ttttccagcg ccacctcgcc ccgattggcc
     1981 tggcgaatat gagccaccac cgaacaggat tttatcgggt agtcctggac aagtttggcg
     2041 atgggggaat taaagctggg ttgtggatct ttaaatgtgc gacagccttt accttcgtaa
     2101 aaggtaatgc cccagccatc tttatgtggc ccggttccac caccacgctg caccagaccg
     2161 gtgaaactaa agcagatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg
     2221 ggtaacctcc acaacagcgg gagcgcatgg cccccggcga agattgattt aagaattttc
     2281 aggccggatg cggcgtaaac gtcttattcg gcctacggtt cgcgtacaga tttgtaggca
     2341 tgataagacg catcagcgtc gcatcaggca tcagcgcaca agtgccggat gcggcgtaaa
     2401 cgccttatcc ggcctacgcc agacttactt aaccatctct ttttcaatta actggatcag
     2461 gatatggatc actttaatgt gaatctcctg aatgcggtcg gcataaccaa agtgcggaac
     2521 gcgaatttcg atatccgccg tgccagccat tttaccgccg tctttaccgg tcagggtgat
     2581 cactttcatc cccttctcac gcgccgctgc gatcgctttg atcacgtttg cagagttacc
     2641 ggaggtggag atccccagca gtacatcgcc ttcgcgacct accgcttcaa cgtagcggga
     2701 gaaaatatca ttgaaaccga aatcattacc gacgcaggaa atatgactaa cgtcagaaat
     2761 agcaatcgcc gggtagcccg gacggttttc acggtagcga ccggtcaact cttcggcaaa
     2821 gtgcatagcg tcgcaatggg aaccgccgtt gccgcaggaa agcactttgc caccggcttt
     2881 aaagctgtct gctaacagga ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt
     2941 taaaaagtta gccagcgttt ccgccgcttc gttcagttcg ttacgaataa gatcctggta
     3001 catgaggata tccttcagca taaatgtaat agacaaaatg cagtgtaccg gataccgcca
     3061 aaagcgagaa gtacgggcag gtgctatgac caggactttt tgacctgaag tgcggataaa
     3121 aacagcaaca atgtgagctt tgttgtaatt atattgtaaa cacattgcta aatgttttta
     3181 catccactac aaccatatca tcacaagtgg tcagacctcc tacaagtaag gggcttttcg
     3241 ttatgatgat tttgagtatt ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc
     3301 gcgtgagctt atttatcagc agtctgattt tgctcgcctg gacagccgcc ctcggcgttg
     3361 ctggtctgtg gtcagcgtgg gtactggtgc ctctggccat tatcctcgtg ccatttaact
     3421 ttgcgcctat gcgtaagtcg atgatttccg cgccggtatt tcgcggtttc cgtaaggtga
     3481 tgccgccgat gtcgcgcact gagaaagaag cgattgatgc gggcaccacc tggtgggagg
     3541 gcgacttgtt ccagggcaag ccggactgga aaaagctgca taactatccg cagccgcgcc
     3601 tgaccgccga agagcaagcg tttctcgacg gcccggtaga agaagcctgc cggatggcga
     3661 atgatttcca gattactcat gagctggcgg atctgccgcc ggagttgtgg gcgtacctta
     3721 aagagcatcg cttcttcgcg atgattatca aaaaagagta cggcggactg gagttctcag
     3781 cttatgccca gtctcgcgtg ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta
     3841 ccgtcggcgt gccgaactca ttaggcccgg gcgagctgct gcaacattac ggcactgacg
     3901 agcagaaaga tcactatctg ccgcgtctgg cgcgtggtca ggagatccct tgctttgcac
     3961 tgacaagccc ggaagcgggt tccgatgcgg gcgcgattcc ggacaccggg attgtctgca
     4021 tgggcgaatg gcagggccag caggtgctgg ggatgcgtct gacctggaac aaacgctata
     4081 ttacgctggc accgattgcg actgtgcttg ggctggcgtt taaactctcc gacccagaaa
     4141 agctgctggg tggcgcagaa gatttaggta ttacctgtgc gctgatcccg accaccacgc
     4201 cgggcgtgga aattggtcgt cgccacttcc cgctgaacgt accgttccag aacggaccga
     4261 cgcgcggtaa agatgtcttc gtaccgatcg attacatcat cggtgggccg aaaatggccg
     4321 ggcaaggctg gcggatgctg gtggagtgcc tctcggtagg ccgcggcatc accctgcctt
     4381 ccaactcaac cggcggcgtg aaatcggtag cgctggcaac gggcgcgtat gctcacattc
     4441 gccgtcagtt caaaatctct atcggtaaga tggaagggat tgaagagccg ctggcgcgta
     4501 ttgccggtaa tgcctacgtg atggatgctg cggcatcgct gattacctac ggcattatgc
     4561 tcggcgaaaa acctgccgtg ctgtcggcta tcgttaagta tcactgtacc caccgcgggc
     4621 agcagtcgat tattgatgcg atggatatca ctggtggcaa aggcattatg ctcgggcaaa
     4681 gcaacttcct ggcgcgtgct taccagggcg caccgattgc catcaccgtt gaaggggcta
     4741 acattctgac ccgcagcatg atgatcttcg gacaaggagc gattcgttgc catccgtacg
     4801 tgctggaaga gatggaagcg gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt
     4861 tcaaacatat cggtcacgtc ggtagcaaca aagttcgcag cttctggctg ggcctgacgc
     4921 gcggtttaac cagcagcacg ccaaccggcg atgccactaa acgctactat cagcacctga
     4981 accgcctgag cgccaacctc gccctgcttt ctgatgtctc gatggcagtg ctggacggca
     5041 gcctgaaacg tcgcgagcgc atctcggccc gtctggggga tattttaagc cagctctacc
     5101 tcgcctctgc cgtgctgaag cgttatgacg acgaaggccg taatgaagcc gacctgccgc
     5161 tggtgcactg gggcgtacaa gatgcgctgt atcaggctga acaggcgatg gatgatttac
     5221 tgcaaaactt cccgaaccgc gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg
     5281 gacgtcatta tctggcacct tctgacaagc tggatcataa agtggcgaag attttacaag
     5341 tgccgaacgc cacccgttcc cgcattggtc gcggtcagta cctgacgccg agcgagcata
     5401 atccggttgg cttgctggaa gaggcgctgg tggatgtgat tgccgccgac ccaattcatc
     5461 agcggatctg taaagagctg ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc
     5521 acaacgcgct ggcgaagggg ctgattgata aagatgaagc cgctattctg gtgaaagctg
     5581 aagaaagccg tctgcgcagt attaacgttg atgactttga tccggaagag ctggcgacga
     5641 agccggtaaa gttgccggag aaagtgcgga aagttgaagc cgcgtaattg aataacggag
     5701 ccgaaaggct ccgtttcttt atccgctaat tatttaaaat taaagccatc cggatggttt
     5761 tccaggctgc cggtcaacgc cgcgaacagc accgttttac catcaatcga aagcgcatcg
     5821 ttcacattca gccaggtgag tttctcttgc gacgttttct catcaatagt cgagaacagc
     5881 cccgtcatct gattagattt ctcggaccac atcacagcga tacgttgcga gccacagtca
     5941 tgcggtttgc acgcgctcat cacctgatac gtatcatctc ccaacgttac ggtttgtgcg
     6001 ggagtataag taccgccttt catcacccag gcaggcagct tatgcccttg taccatctga
     6061 ttaaatgcag ctttggtggt ttcgcccttt gcaaggctgc taatggttaa atcatcctgc
     6121 gccattgcac tggtggcgat gaccagagcg gcgactgtcg ttattgtctt aaacatcatt
     6181 cctcccgagc ttatcctgcc catgttatta accttctaaa gcctggcgat aacacgataa
     6241 tttgcaaatc agatatttcc aatatcagca tttgttatca atttattctg cgctaaggca
     6301 actgcctgat aaggtgttaa agttataaaa cccttcgcat aaaggagttg tacaccgtgc
     6361 ctggtttgaa gattacgctt ttgcagcaac cactggtgtg gatggatggc cctgccaacc
     6421 tgcgtcattt tgatcgtcaa ctggaaggta ttaccgggcg cgatgtgatc gttctaccga
     6481 agatgtttac cagcggcttt gccatggaag cggcagcttc gtcgctagca caagatgacg
     6541 tagtggactg gatgacagct aaggcgcagc agtgcaatgc gctgattgca ggcagtgttg
     6601 cattacaaac ggagtctggt tcggttaacc gctttttgct ggttgagccg ggcggcacgg
     6661 tacattttta tgataagcgt catctgttcc gcatggcaga tgagcatcta cattataaag
     6721 cgggcaatgc gcgagtgatt gtggcatggc gcggctggcg tattttgccg ttggtgtgct
     6781 acgacttacg ttttccggtg tggtcgcgca atctcaacga ttatgacctc gccatctacg
     6841 ttgccaactg gcctgctccg cgctctctgc actggcaggc attgctgacg gcccgcgcga
     6901 ttgagaatca ggcgtatgtg gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc
     6961 attatcgcgg tgacagccgg gtgattaatc cacaaggcga gattatcgct actgccgacg
     7021 cgcatcaggc aacgcgcatt gatgcggagc tgtcgatgat ggcattgcgg gaatatagag
     7081 aaaagtttcc ggcatggcag gatgcggatg agtttaggtt gcggtgagtg ggggttgtgt
     7141 ttaaggctgg gggagagtcg gggtaagact acgaaagccc gctctccgca aggactgacg
     7201 ccagatagtt tctgtccata gctgcttttc gcatcttacg tcttaaccct gccttgaata
     7261 ccttatcatt cgtcaaaata ttaatagcga tgtgccgtat ccctgaaaat aattctgctg
     7321 catttcctct tcttattttg cagtcgtctt cattcattac cacgtccaga cgccagtgca
     7381 gcttattctc cacgtgccag tggtttcgga ttgctgtggc gaacttctct gcggttaaat
     7441 cagcagaact gatataatat ctgaccgtca tttctggctc tttcttttgt tctgctatta
     7501 tggaccgaaa ggagactgcc acgcataatt tcttcagccc tttccattca aacgtgaaat
     7561 caataagttc atcagggaca tcgcaaacaa tatgaagacg gatttcttct ctgccgtgac
     7621 tcttttcact gattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt
     7681 tttcctcaaa ggctttatta agccgcccct ggtttccttt taccgcgaat aaataatcac
     7741 cgccctgttt
//
LOCUS       DAZOZD010000196         7746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOZD010000196 DAZOZD010000000
VERSION     DAZOZD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.196"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..116
                     /locus_tag="ACHUPB_003410"
     CDS             <1..116
                     /locus_tag="ACHUPB_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFR0760139.1"
                     /translation="EKLSIPFIYLLFQWSFPHLHSDGMTVVIRPLQPPHTN"
     gene            150..1217
                     /gene="pilL"
                     /locus_tag="ACHUPB_003411"
     CDS             150..1217
                     /gene="pilL"
                     /locus_tag="ACHUPB_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HFR0760140.1"
                     /translation="MKKNCLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAQPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1217..1654
                     /gene="pilM"
                     /locus_tag="ACHUPB_003412"
     CDS             1217..1654
                     /gene="pilM"
                     /locus_tag="ACHUPB_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR0760141.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1668..3350
                     /locus_tag="ACHUPB_003413"
     CDS             1668..3350
                     /locus_tag="ACHUPB_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR0760142.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNGRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3343..4638
                     /gene="pilO2"
                     /locus_tag="ACHUPB_003414"
     CDS             3343..4638
                     /gene="pilO2"
                     /locus_tag="ACHUPB_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR0760143.1"
                     /translation="MADEDINPVVLLEDPKVNHRVWAACLKWTPVVKKQRVPSHHKHK
                     SHVKPRRLTSLKVTVGSRNSRGKISRLTGTGILTRPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLAMNEEPPEKWQVVSSLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAAGAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFGVIPDFNLKDGARQVSVTRPLPSLPGRDEA
                     VPAPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDEFFPLLQDTGVRLSNIHFELNGGTFSYSSEGHIYASK"
     gene            4625..5077
                     /gene="pilP"
                     /locus_tag="ACHUPB_003415"
     CDS             4625..5077
                     /gene="pilP"
                     /locus_tag="ACHUPB_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR0760144.1"
                     /translation="MRPSKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTAPLPSVSEQTAPKNRKELPIIMEINGKD
                     KRLHAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5088..6641
                     /locus_tag="ACHUPB_003416"
     CDS             5088..6641
                     /locus_tag="ACHUPB_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR0760145.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PGINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWETRAPELRDD
                     ERDYLERHCNKDSLCSTDNIWFRNPHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            6654..>7746
                     /locus_tag="ACHUPB_003417"
     CDS             6654..>7746
                     /locus_tag="ACHUPB_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR0760146.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTQLIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGN"
BASE COUNT         2217 a         1934 c         1754 g         1841 t
ORIGIN      
        1 acgaaaaatt atcaattcct tttatatatc tcttatttca gtggtctttc ccgcatttgc
       61 attcggacgg aatgacagtc gtcattcgtc ctctccagcc gcctcacacc aattaacaaa
      121 cacgtatata cgaacacgga gtctccgtta tgaaaaaaaa ctgtctgaca cttatactgc
      181 cgtcattatt gtccggctgt gccggaattt ccggcagcaa taacgtggca ccatcagata
      241 cgcctgctct ggtttttgtt gacggacaaa tcagtgaaag tgctggcgtt ctggcactta
      301 cgcaacaacg tctttctcca cctaaaataa ctcccccccc gagagtcagc gtgccagaga
      361 cagtaaaaac agcacagcct gttagcgaat cccgaccgtt accttcacca acaataagtc
      421 agaatttctc ccggttaatc attacaggca agcctggtag cgcccctgta acactatcgc
      481 cgacggcccg taaccggacc gtcagtcagt ggataaaaac attactaccg gaaggatgga
      541 aattcagaca agagaatgca gccacgccca agctgaatac caaactggtt tcatggtctg
      601 ccaatgatca gtggacccgt tctctgaacc ggttgctgga agaacaacac ctatggggcc
      661 atctggactg ggataataaa acgttgaccg tcacaacatc agctgctgca ccggcacagg
      721 acacggcaac cactatctcg ccagcagcca tggtaaccac atccacttcc cctgaatctc
      781 caacaacagc aaatagccag aacaaaccac gaaatccatt cagaggaaac agcgtatctt
      841 cgtcaacccc cgccgccacc ccaactccaa ccggctccac agtcaagtct atcccgctca
      901 tgaccgggac gcctgtcaaa ccggtatctc agggtaaaga gtggcgtgcg ccggcaggca
      961 ccacattaag agagaacatt attaagtggg cagaagaaac aaaatgcgaa agcatggcat
     1021 caacgcactg gatggtcata tggccacttt ctgtaaccga ttaccgtctg gatgcgcctt
     1081 taatgttccg gggatcattt gaatcagcca tggagcaggt atttgagtta tatcggactg
     1141 cgcaaaaacc tttatacgca caggcttctc gcatgcagtg catcattaca gtttctgata
     1201 caagggatgg tcgctaatgg gatggctggt catggccgtc ggcctgacca ttctgattat
     1261 cactggtagc taccagaatc agaagatgtc tgaaaccaca aacgcccagc aatatgcaag
     1321 tgcgtctgta tgggcaagtc agatactaat gattgctaac agaatcaatg atatcagata
     1381 cgtatcaggc cagcaggacg gagtaatttc ctcagataaa ctggctctcc ctgtcactcc
     1441 ggactcccga attaaacatc agttacaaca ggggcgtctg tgggtatgga tgcctgaaca
     1501 accaggactt gttgaaactt tgcgttcaaa aagtcgtggt tctgcactaa tagggatctt
     1561 ccaaaacggg caactcacct ggctatcagg aactgcaacc ggtctgacac ccccggcagg
     1621 cattactgct ggttctgtcg tctacgttaa ctaatctgga tatattcatg aaaaaatcac
     1681 accagcgttc aatgaagctg gcggtgctcc cctgcatgat cgccgtcgct ctttccatct
     1741 ccggatgtac tttcagcgaa atcaacaaaa tgcagaaaaa agcacaggaa gactcagcac
     1801 atgcacggga aaaggtatca gccctttcgg cccgtaaatc gcaggctctc acctggctcg
     1861 ataatcaatg gataaaccct gttccggtcg ctcaggtatc aagagagaaa aaacaaacag
     1921 ctccggcctg ctacatcacg caggcaagaa aaggagagat cactctgcag gaactggggc
     1981 aacgtattac tgccgtatgc ggcatccctg taatcatcac gcctgacgca gccaattcaa
     2041 ctcttgaagg aggcgctacc cgccagatga caggaacact accagcacca gatgaaaatg
     2101 gacgtctacc gttaagcagc ctgggcagca caacaatgac tacctccact cagccattaa
     2161 cgctgaataa cctcatgtgg cagggagata tcaatggtct tctggatctg atggccagcc
     2221 ggagtggtct gtactggcgc atggataatg gtcggattgt attctatctg actgaaacca
     2281 gaacgtatcc acttcatatg ctgaacacca aaaccagcag cagttccagt gtcagctctg
     2341 gctcaacaag cacaatgggg gcaacaggag gccaggataa ctcagcatcc ggtgatgcaa
     2401 cgtcctctca gagcacaacc gttggtcagg aatacgatct gtatgaagac atccggaaaa
     2461 ctattgaagc aatgctgaca ccagaaaaag gccgttactg gttatctgca tcgagctcaa
     2521 cgctaacagt cactgatact cctgctgtcc aggaagccgt cgcacgatat gtggacgaac
     2581 aaaacagtat tatgaaccgc caggtagccc tgaacgtaca ggttctgagc gtcagcaata
     2641 ccagaaacga gcagttcggt ctggactgga accttgttta caaatcgcta cattccgccg
     2701 gagcaacgtt gaacaatgca agcggagatt ttacaggcgc tacatctgca ggcgtatcaa
     2761 ttctggatac ggcaacaggg aatgccgcca aattcagcgg ttccagcctt ctgattaaag
     2821 cactgagtga acaaggcgat gtcagcgttg taacttcaca agaaagcact gtcacaaacc
     2881 tgacgccggt acctatccag atggcagatc agacagttta cgtcgcccag tcagcaacaa
     2941 ctacgactac ggatgtagga gcaacaacaa cattaacacc gggcatgatc accactggat
     3001 tcaatatgac cctactgcct ttaattcaga aaacaggcaa tctccagttg cagatgaact
     3061 ttaatctgtc tgatccccca actatccgta gctttacgtc aaaagacgga aacagttaca
     3121 tcgaaatgcc atataccaaa ctgcgttcac tgagccagaa ggtcaatctg aaagaagggc
     3181 aatcacttgt cgttactggt ttcgatcaga acaatacgac gacaagtaaa gccggtacgt
     3241 ttacgccagc aaatccatta tttggtggtt cacaaaccgg aaaaaatgaa cgcagcacgc
     3301 ttgtgatcat cattaccccg actttcccgt caggaggcaa taatggctga tgaagatatc
     3361 aacccggtag tgcttctcga agatcctaaa gtaaatcatc gcgtgtgggc tgcctgtctt
     3421 aaatggactc ctgtcgtaaa aaaacagcgc gtcccctcac atcataaaca caagtctcat
     3481 gtaaaaccca gacggcttac ctccctgaaa gtgactgtag gcagcagaaa ttcccgtgga
     3541 aagatcagcc gccttaccgg cacaggaata ctgaccaggc cagaacgcaa tcattatttt
     3601 tccctggcac tggctttctg ctcatgggtc agaaatggat acggtgtctt ccggtacagc
     3661 gataaggagc tcttattcct tgcttccatt aatggccagc ctgccgttat ggctgactta
     3721 tcaggaaatg atgccgacgt tgcccaaaag gtcagccttt ttctggcgat gaacgaagag
     3781 ccccccgaaa aatggcaggt tgtatcgtct ctggaacatc ctgataactg ggaaagtatt
     3841 attacccggt tatcatcagc agacctccgc cgttgcaaac ttactgtcgg caatcgcagc
     3901 aaattcacac tgcctgccgt actgttttta gtagcagccg gcgcaggcac tgtattctgg
     3961 atgactcagc cagagccgga tgtcgtaccg acgcccgagg agatagctgc tcgggcaaga
     4021 ttgcagttta aaaagcccga tccaccgcca gaactcccgc atccatgggc atcacaacct
     4081 gtaatcagtg attttctgaa agcctgcgcg gaccttcgaa aaccgtcacc ggtcgcactg
     4141 gaaggctgga aacttaccgg cggaacatgt actcctgaga cattcacact gatttacgaa
     4201 cgacagcctg gaggaaccat tgagggtttt ctggcaagaa gtaaagaaat ctttggtgtt
     4261 attcctgact ttaaccttaa agatggtgca cgccaggttt ctgtaacccg tccactgccg
     4321 tccctgcccg gcagggatga ggctgtcccg gccccctcag aacaactgat gcgggtgttc
     4381 acgtggtttc agaaaaaaca gttaacaccg gccattaatg aaatagccat accggagcca
     4441 ttaccaggca acgatggtga accagcccct gttcagaaat ggaaagaata tcagttcagt
     4501 ttatcaactc ctgtaaatcc tgatgagttt ttccctttac ttcaggatac cggcgtccgt
     4561 ctgagcaata tccattttga attaaacggc ggtactttca gttattcctc agaaggtcat
     4621 atatatgcgt ccaagtaaat tactcattat tccttccata tttttcttct ctggtttttc
     4681 atttgccaca acacaacctc ttgtcacaat aggagaactt gaagcacaac aaaaccggaa
     4741 tattctttta caggcaaaag ttcagggtgc acagttacag aaacaacttg aagaatcaga
     4801 tgttacttca tcatctgaaa ctgtttcagg tttctctggt gtgaccgccc ccttgccatc
     4861 agtttcagag caaacggcac cgaaaaacag aaaagaattg cccatcatta tggaaataaa
     4921 cggcaaagat aaacgtcttc atgccgttct tcgcatggct gatggacgtc agacaagcgt
     4981 caccactggc agccagcttc caggtacatc cgtcacggtg aagtcgatct cattgtctgg
     5041 cgtcacgtta agtgatggca ctacccttac tttctgagta acgtgatatg gataaatata
     5101 atctgaagga tgctctcctt tttatatccc ggggagacac ccatgaaatt cttatagaaa
     5161 caaaccagcg gacaaggcct gacgtacaaa gtaatctcca ggaactgcta aaattatatc
     5221 cgggtatcaa cccaaaagta gtctcgttgt cggagttaca ggaagcaggt caggatgaaa
     5281 aaaacagagg cccgaaagaa cgaattattc atctgaaaga tcttgctgat gtcagtgaaa
     5341 gtgagaagaa agttctatct tattttgaaa cggccagaaa acttggtgct tcagatattc
     5401 attttttaat ttctgagtct attttcaaag ttcgcatgag aatattcggt gaactgcaga
     5461 ctgtagatga agatcaacca gcactgggct attcactttg cgcaacagcc attctgtcaa
     5521 tggctgatgt cactgaaacc agtttcttcc ctcaaaggga acaggatgcg cgcctgtccc
     5581 cacaattaat gcgaaaaata ggaatatttg gtgcacgcta cagtcacaga cctacgggag
     5641 acggtctgat tgcagttatg cgcctgatac ccgatgacgg agataaagtt cccacgttta
     5701 aacaactggg ctttatcccg gaacaaatcc ggttgctgaa cataatgctg cgccgcccgg
     5761 aaggaaaaat agtcctgtca ggtccaaccg gttccggtaa atcgacaacg ttacgcagcg
     5821 cctgccgtgt atatcttgac gataatcagg ggcgacacct gctaacaatc gaagatccgc
     5881 tggaaggaca gatactggga gcaacacaga ctccgattat ctgcgacaaa tccgacgaag
     5941 atgccgtcaa actggcatgg agtcgggcta tttcatcggc aatgcgactt gaccctgatg
     6001 ccatcatgga aggggaaatg cgcgatctta tctcgatgat gtcaaccacc tacgcggcac
     6061 agaccgggca catagttctg acgacattac atacaaactc agctctgggt ataccggagc
     6121 gaatgattac gatgggtatg aatgccgatc ttatttgcga tgcgcaatta ctgattggga
     6181 tgatcagcca gcgccttgta ccaactctgt gtccctcctg ccgtattcca tgggaaacgc
     6241 gagcacctga acttagagac gacgagcgtg attaccttga gcggcactgc aataaagatt
     6301 cactctgcag tacagataat atctggttcc gaaatcctca cggatgctct gaatgtaatc
     6361 acgacgtaat cattaacggc agaaagcgcg gtgaaatagg taaaggcctc accggcagga
     6421 ctgtaattgc tgaggtgata gaacctgata accgcttatt tcagattctg aaaacacgcg
     6481 gcaaagttgc agcacgtaaa tactggctgg aaaacatgaa aggcatcagt cgtgtagagc
     6541 atcttcttcg ccgtatcaat gaaggtctgg tagaccctct tgaagccgac agaatcatcc
     6601 ctctggacga agacgagcgc ctcagcatcg atgatgtcta acaggagaac aacatgcgtg
     6661 aaatgaattt cagtcaacgg ctgaggcgtt tcattgtcag aaaaacgttc agcgccccct
     6721 acagggtgca gttttacgaa gccctgcgtt ttctgctgga aaataaacag ccattaaaga
     6781 ccgcactgga acagatgcgg gatgcctgga cagactttgg acgaaaatgg catccctttg
     6841 ctgaactggc cacagactgc attgagtctc ttcgtgaaaa cagtggcgaa aactctctgg
     6901 aatatactct tagtttatgg gttcctcagg aagaggccgc agtaatcagc gccgggataa
     6961 gaagtggttc aattgtggat gcactgcaat tcgccaccac tcttacagat gcaaaagaac
     7021 aaatccatca ggctatatgg caaatggcta tttatccggt gggattgtta atcatgatga
     7081 cggggacatt atatgttctg aatacacagc taatacctga actgagcaaa ataagctccc
     7141 ccgattcatg gagtggtgct cttggttttc tttatggatt atctgttttt gtcgataatt
     7201 acggcgctat atgtgccgtt ctttttgcag tgatcacagg tttaatatca tggtctctgc
     7261 caaactggaa aagccctgac agtgttcgta cttttgccga caagataatg ccatggagta
     7321 tttatcagga tatacaggga gcaacatttc tcctgaatat ggctgcactt ctgaaagcca
     7381 aaatgacgac gctcaactca ctaaacattc ttcaggagtt tgcatcacca tggttaagca
     7441 cacgtcttga cagcattatt taccgcgtcc gccaggggga tcatcttgga ctggctttgc
     7501 gtcagtgcgg ttatcagttc ccatccaggg aagcagccaa cttcctgtct ttattacagg
     7561 gagatggcgc aacagagcta atcagcaatt atggtcaacg atggcttagt caaacacttc
     7621 aacgtgtaaa gaaacgggca aacgtgattc gtttaattat gctgatattt cttgtcatgt
     7681 ctctcatgtt actggttttt gccattatgg atattcagtc tattagtgac aacagcatgg
     7741 gtaact
//
LOCUS       DAZOZD010000197         7715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOZD010000197 DAZOZD010000000
VERSION     DAZOZD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.197"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUPB_003418"
     CDS             <1..643
                     /locus_tag="ACHUPB_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0760147.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUPB_003419"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUPB_003419"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR0760148.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUPB_003420"
     CDS             2474..2986
                     /locus_tag="ACHUPB_003420"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0760149.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUPB_003421"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUPB_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0760150.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUPB_003422"
     CDS             5579..5782
                     /locus_tag="ACHUPB_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0760151.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUPB_003423"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUPB_003423"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0760152.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHUPB_003424"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHUPB_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0760153.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZOZD010000198         7602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOZD010000198 DAZOZD010000000
VERSION     DAZOZD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.198"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1593
                     /locus_tag="ACHUPB_003425"
     CDS             <1..1593
                     /locus_tag="ACHUPB_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0760154.1"
                     /translation="DSTTTITTNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLG
                     EVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRRNNCYQFIVII
                     NSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVFDIK
                     FETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGE
                     NFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQPYLVINKDAV
                     TPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTR
                     MKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNAL
                     DPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLG
                     INQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKS
                     YQHEKNRNGLMPLIRRRTMLQL"
     gene            1783..1896
                     /gene="yhiY"
                     /locus_tag="ACHUPB_003426"
     CDS             1783..1896
                     /gene="yhiY"
                     /locus_tag="ACHUPB_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0760155.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1959..3026
                     /locus_tag="ACHUPB_003427"
     CDS             1959..3026
                     /locus_tag="ACHUPB_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0760156.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            3023..5758
                     /gene="rbbA"
                     /locus_tag="ACHUPB_003428"
     CDS             3023..5758
                     /gene="rbbA"
                     /locus_tag="ACHUPB_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0760157.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5758..6882
                     /locus_tag="ACHUPB_003429"
     CDS             5758..6882
                     /locus_tag="ACHUPB_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0760158.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6955..7228
                     /locus_tag="ACHUPB_003430"
                     /pseudo
     CDS             6955..7228
                     /locus_tag="ACHUPB_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            7225..7584
                     /locus_tag="ACHUPB_003431"
     CDS             7225..7584
                     /locus_tag="ACHUPB_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0760159.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
BASE COUNT         1878 a         1808 c         2062 g         1854 t
ORIGIN      
        1 gacagtacaa cgacaatcac aacaaatgat atggtgcaag agcatgttac caacttcact
       61 aaagaactgt ttgggtatat tgcaaatggt ataggtgatg atatatcaag tattgccagg
      121 acaatgctcg gtgaggttgt ggaaaaaata gatgattggc aaatagaacg gttccaacaa
      181 tctattcaag atgacaaaat ttcatttacc atacaaacga atcattcaga aaaatatagt
      241 atgctatcgg gcatgcgcgc tcatattcta cgtaggaata actgttatca gtttattgtg
      301 ataattaata gtaaaaatta cggttgccct cttgataata ccgatattaa ttggtgcagc
      361 attgtttatt tacttaataa tatgacagta aatgataatg ctaatgatgt tgcagttact
      421 gagtcgtaca aacccatctg gaactggaaa atatcgcaat acaatgtatt tgatattaaa
      481 ttcgaaacaa tgataaaacc ccagttcgct gacagaacct atttctccaa ttgttcacct
      541 gttgatccca caagtaccag accgacctat tttggcgata ccgatggctc agtcggtgca
      601 gttttgtacg cgctttttgc aacgggacat ttgggaatca tggctgaagg agaaaatttc
      661 cttagccagt tgcttaatat agaagatgaa gttctcaacg tattgctaag ggagaacttt
      721 aatgagcaac ttgacactaa tgttaatata ataatcagta tcctgaacag aagagacatt
      781 gttttagaaa gtcttcagcc ttatttggtt attaataaag acgcagtcac gccatgtacg
      841 tttctgggcg accagactgg cgatcgattt agtaatatct gtggcgataa atttatcatc
      901 gatttattaa aacgcataat gagtatcaat gaaaacgttc atgtattagc tggaaatcat
      961 gaaacgaatt gcaatggtaa ctatatgcag aacttcacgc gcatgaaacc acttgatgag
     1021 gatacctact ctgggataaa agattatcct gtatgtttct atgaccccaa atacaaaata
     1081 atggcaaatc atcatgggat aacttttgat gatcagcgaa aacgttacat tataggaccg
     1141 ataacagtct ctagtgatga aatgaccaat gctctcgatc cggtcgaatt agcggcaatt
     1201 atcaataaaa agcaccatgc tgtaattaat ggcaaaaagt ttaaaaatag ccgggctatc
     1261 tcttgtcgat cctttaatcg ttatttttct gtttcgacag attacagacc taaacttgaa
     1321 gcactattag catgctcaca aatgttaggt ataaatcaag ttgttgcaca taacggtaat
     1381 ggtggaagag aatgtatagg tgaaacgggg acagttttag gtcttaacgc ccgtgacagt
     1441 aaacatgctg gaagaatgtt cagcatgcat aattgccaaa ttaaccctgg tgcagggcct
     1501 gagataacca ctccctggaa gtcttaccag catgaaaaaa acagaaatgg acttatgcct
     1561 ttgatcagaa ggcgcacaat gttacagctc tgaaccatag acaacatgaa tattaatgtt
     1621 actatctggt tgagactgtt gataaaacgt aaaaaggaat gctctgttcc tgcaactgaa
     1681 caggcgtgaa acgctatcca acaggatgga taccgttttg tcagccagtt ttatagatca
     1741 cacttatgag tatgcgtgtt tcatttacgg ccaggaatgc tgatgatgac aacattacta
     1801 cccgttttca ctaagccttc cccgttggcg ctcaatgctc tgcgcgctgg tcgtatttgt
     1861 cgtttccttc ttatcccgga tggaagaatc cgctgaacgt cgttcggcgg cagttgtaac
     1921 acgcgtcgtt tgcttgcagg gattatggcg ggtcgattat ggataagagt aagcgccatc
     1981 tggcgtggtg ggttgtcggg ttactggtgg tggctgccgt cgtggcgtgg tggctgttgc
     2041 gcccggcggg tgtgccggaa ggctttgctg tcagcaatgg gcgcattgaa gcgacggaag
     2101 tggatatcgc cagcaaaatt gccgggcgta tcgacaccat tctggtgaaa gaaggccagt
     2161 ttgttcgcga aggcgaagtg ctggcgaaga tggatactcg cgtgttgcag gagcagcgac
     2221 tggaagccat cgcgcaaatc aaagaagcac aaagtgccgt tgctgccgcg caggctttgc
     2281 tggagcaacg gcaaagcgaa actcgtgccg cgcagtcgct ggttaatcaa cgccagtccg
     2341 aactggactc cgtggctaaa cgtcatacgc gttcccgctc gctcgcccaa cgaggggcta
     2401 tttctgcgca acagctggat gacgatcgcg ccgccgctga gagcgcccga gctgcgctgg
     2461 aatcggcaaa agctcaggta tcggcttcta aagcggctat agaagcggcc cgtaccaata
     2521 tcattcaggc ccaaactcgc gtcgaagccg cgcaagccac tgaacggcgt attgccgcag
     2581 atatcgatga cagtgaactg aaagccccgc gtgacggacg cgtgcagtat cgggttgctg
     2641 agccagggga agtgctggcg gcaggcggtc gggtgctgaa tatggtcgat ctcagcgacg
     2701 tctatatgac tttcttcctg ccaaccgaac aggcgggcac gctgaaactt ggcggtgaag
     2761 cccggctgat cctcgatgcc gcgccagatc tgcgtattcc ggcgaccatc agttttgtcg
     2821 ccagtgtcgc ccagttcacg ccaaaaaccg tcgaaaccag cgatgaacgg ctgaaactga
     2881 tgttccgcgt caaagcgcgt atcccaccgg aattactcca gcagcatctg gaatatgtca
     2941 aaaccggttt accgggcgta gcgtgggtgc gggtgaatga agaactttcg tggcctgacg
     3001 acctcgtggt gaggttgccg caatgacgca tctggaactg gttcccgtcc cgcctgtcgc
     3061 gcaactggcg ggcgtgagcc agcattatgg aaaaaacgtt gcgctgaaca atattactct
     3121 cgatattccg gcccgctgta tggtcgggct gattggtccg gatggcgtcg ggaagtcgag
     3181 cttattgtcg ttgatttccg gtgcccgcgt cattgagcag ggcaatgtga tggtgctggg
     3241 cggcgatatg cgcgacccga ggcatcgccg cgacgtctgc ccgcgcatcg cctggatgcc
     3301 gcaggggctg ggcaaaaacc tctaccacac cttgtcggtg tatgaaaacg tcgatttttt
     3361 cgctcgcctg ttcggtcacg acaaagcgga gcgggaagtg cgaattaatg agctgctgac
     3421 cagcacgggg ttagcaccgt ttcgcgatcg tccggcaggg aaactctccg gcgggatgaa
     3481 gcaaaaactt gggctgtgct gcgcgttaat ccacgacccg gaactgttga tccttgatga
     3541 gccaacaacg ggggttgacc cgctctcccg ctcccagttc tgggatctga tcgacagtat
     3601 tcgccagcgg cagagcaata tgagcgtgct ggtcgccacc gcctatatgg aagaggccga
     3661 acgcttcgac tggctggtag cgatgaatgc cggagaagtg ctggcaactg gcagcgccga
     3721 agagctacgg cagcaaacgc aaagcgctac gctggaagaa gcatttataa atctgttacc
     3781 gcaagcgcaa cgccaggcgc atcaggcggt agtgatccca ccgtatcaac ctgaaaacgc
     3841 agagattgcc atcgaagcgc gcgatctgac catgcgtttt ggttccttcg ttgccgttga
     3901 tcacgttaat ttccgcattc cacgcgggga gatttttggt tttcttggtt cgaacggctg
     3961 cggtaaatcc accaccatga aaatgctcac cggactgctg cccgccagcg aaggtgaggc
     4021 gtggctgttc gggcaaccgg ttgatccaaa agatatcgat acccgccgtc gggtgggcta
     4081 tatgtcgcag gcgttttcgc tctataacga actcaccgtg cggcaaaacc ttgagttaca
     4141 tgcccgtttg tttcacatcc cggaagcgga aattcccgca agagtggctg aaatgagcga
     4201 gcgttttaag ctcaacgacg ttgaagatgt tctgccggag tcattgccgc tcggcattcg
     4261 ccagcggctt tcgctggcgg tggcggtgat tcatcgcccg gagatgttaa tcctcgatga
     4321 gcctacttct ggtgtcgatc cggtggcgag ggatatgttc tggcagttga tggtcgatct
     4381 ctcgcgccag gacaaagtga ctatcttcat ctctacccac tttatgaacg aagcggaacg
     4441 ttgcgaccgc atctcactga tgcacgccgg aaaagtgctt gccagcggta caccgcagga
     4501 actggttgag aaacgcggag ccgccagtct ggaagaggca tttatcgcct atttgcagga
     4561 agcggcaggg cagagcaacg aagccgaagc gccgcccgtg gtacacgaca ccacccacgc
     4621 gccgcgtcag ggatttagcc tgcgccgtct gtttagctac agccgccgcg aagcgctgga
     4681 actgcgacgc gatccagtac gttcgacgct ggcgctgatg ggaacggtga tcctgatgct
     4741 gataatgggt tacggcatca gtatggatgt ggaaaacctg cgctttgcgg tgctcgaccg
     4801 cgaccagacc gtcagtagcc aggcgtggac actcaacctc tccggttccc gttactttat
     4861 cgaacagccg ccgctcacta gttatgacga gcttgatcgt cggatgcgtg cgggcgatat
     4921 cacggtggcg attgagatcc cgcccaattt cgggcgcgat atcgcgcgtg gtacgcctgt
     4981 ggaactcggc gtctggatcg acggagcgat gcccagtcgt gccgaaacgg taaaaggtta
     5041 cgtgcaggca atgcaccaga gttggcttca ggatgtggcg agccgacaat cgacacccgc
     5101 cagccaaagc gggctgatga acattgagac gcgctatcgc tataacccgg acgtaaaaag
     5161 cctgccagcg attgttccgg cggtgatccc gcttctgctg atgatgatcc cgtcaatgct
     5221 aagcgccctt agcgtggtgc gggaaaaaga gctagggtcg attatcaacc tttacgtgac
     5281 ccccaccacc cgtagcgaat ttttgcttgg caaacagctg ccgtacatcg cgctggggat
     5341 gctgaacttt ttcctgctct gcggcctgtc ggtgtttgtg tttggcgtac cgcataaagg
     5401 cagtttcctg acgctcaccc tggcggcgct gctgtatatc atcattgcca ccggaatggg
     5461 gctgctgatc tccaccttta tgaaaagcca gattgccgcc attttcggaa cggcgattat
     5521 cacgttgatc ccggcgacac agttttccgg gatgatcgat ccggtagctt cgctggaagg
     5581 gcctggacgt tggatcggcg aggtttaccc gaccagtcat tttctgacta tcgcccgcgg
     5641 aacgttctcg aaagcgctgg atctgactga tttgtggcaa ctttttatcc cgttgctgat
     5701 agccatcccg ctggtgatgg gcttaagcat cctgctgctg aaaaaacagg agggatgatg
     5761 cgccatttac gcaatatttt taatctgggt atcaaagagt tgcgcagtct gctcggtgat
     5821 aaagcgatgc tgacgctgat tgtcttctcg tttacggtgt cggtgtattc gtcagcgacc
     5881 gttacgccag gatcgttgaa cctcgcgccg atcgccattg ccgatatgga tcaatcgcag
     5941 ttatcgaacc ggatcgttaa cagcttctat cgtccgtggt ttttgccacc ggagatgatc
     6001 accgccgatg agatggatgc cggactggac gccggacgct ataccttcgc gataaatatt
     6061 ccgcctaatt ttcagcgtga tgtcctcgcc ggacgccagc cggatattca ggtgaacgtc
     6121 gatgccacgc gcatgagcca ggcatttacc ggcaatgggt atatccagaa tattatcaac
     6181 ggtgaagtga acagctttgt cgcgcgctac cgtgataaca gcgaaccgtt ggtatcgctg
     6241 gaaacccgga tgcgctttaa cccgaacctc gatcccgcgt ggtttggcgg ggtgatggcg
     6301 atcatcaaca acattaccat gctggcgatt gtattgaccg gatcggcgct gatccgcgag
     6361 cgtgaacacg gcacggtgga acacttactg gtgatgccga taacgccgtt tgagatcatg
     6421 atggcgaaga tctggtcgat ggggctggtg gtgctggtgg tatcgggatt atcgctggtg
     6481 ctgatggcga aaggtgtact gggcgtaccg attgaaggct cgatcccgct gtttatgctg
     6541 ggcgtggcgc tcagtctgtt tgccaccacg tcaatcggca tttttatggg gacgatagcg
     6601 cgttcaatgc cgcaactggg gctgctggtg attctggtgc tgctgccgct gcaaatgctt
     6661 tccggtggat ccacgccgcg cgaaagtatg ccgcagatgg tgcaggacat tatgctgacc
     6721 atgccgacga cacactttgt tagcctcgcg caggccattc tctaccgggg tgccggattc
     6781 gaaatcgtct ggccgcagtt cctgacgctg atggcaattg gcggcgcgtt tttcaccatt
     6841 gcgctgctgc gattcaggaa gacgattggg acaatggcgt aaggaaggga aacttgactt
     6901 catactgtga tgtcatacta ttgatgtcat aaagtgaagt cataaggaca tgccatgaga
     6961 accaaggtac aggctttacg gaagaaaaaa aaatacattg gatcaaatat ttaaaactcc
     7021 tgttcctcaa gggatcaagt ggtctgatat agagtcactg gttaaagcat taggcggaga
     7081 aattaaggaa ggaagaggtt cgcgttgtaa gttcatacta aatatgagcg ttgcgtgttt
     7141 ccatcggcct catccgtcgc cagataccga taaaggcgct gtagaaagcg tgcgtgactg
     7201 gttactaagt ataggggtaa aaccatgatc aaactgaaga cgccaaactc tatggagatt
     7261 gccgggcaac cagctgttat tacctacgtg ccagaactga atgcatttcg tggcaaattc
     7321 ctgggcttgt caggttattg tgattttgtc tctgacagca ttcaggggct gcaaaaagaa
     7381 ggggaacttt ccctgcgaga gtatcttgag gattgtaaag ctgcgggtat tgagccgtat
     7441 gcgcgtacag aaaagattaa gacctttacg ttacgctatc cagaatcgct gagtgaacgc
     7501 cttaataatg cggctgcgca gcaacaagtc tctgttaata cctatattat tgaaacactg
     7561 aatgagcgtt tgaatcattt ataaatttta gcggcgtaca tt
//
LOCUS       DAZOZD010000199         7595 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOZD010000199 DAZOZD010000000
VERSION     DAZOZD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.199"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..716
                     /gene="gadW"
                     /locus_tag="ACHUPB_003432"
     CDS             <1..716
                     /gene="gadW"
                     /locus_tag="ACHUPB_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0760160.1"
                     /translation="SVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFCSPDTR
                     RLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESGVNSPI
                     LSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAERMYTS
                     ESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYFINTFR
                     QYYGVTPHQFAQHSPGTFS"
     gene            complement(1079..4192)
                     /gene="mdtF"
                     /locus_tag="ACHUPB_003433"
     CDS             complement(1079..4192)
                     /gene="mdtF"
                     /locus_tag="ACHUPB_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR0760161.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4217..5326)
                     /gene="mdtE"
                     /locus_tag="ACHUPB_003434"
     CDS             complement(4217..5326)
                     /gene="mdtE"
                     /locus_tag="ACHUPB_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR0760162.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5434..5712
                     /locus_tag="ACHUPB_003435"
     CDS             5434..5712
                     /locus_tag="ACHUPB_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760163.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5713..6240)
                     /gene="gadE"
                     /locus_tag="ACHUPB_003436"
     CDS             complement(5713..6240)
                     /gene="gadE"
                     /locus_tag="ACHUPB_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0760164.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7039..>7595)
                     /gene="hdeD"
                     /locus_tag="ACHUPB_003437"
     CDS             complement(7039..>7595)
                     /gene="hdeD"
                     /locus_tag="ACHUPB_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0760165.1"
                     /translation="ATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILS
                     TVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIA
                     AFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVST
                     LVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         1990 a         1830 c         1808 g         1967 t
ORIGIN      
        1 gctcggtgat cctcattcgt cgttcattcg atatttatca tgaacagcat aaaatatcgc
       61 tgcataacga gagtatcgtg ctgctggaga aaaatttggc agacgatttt gcgttttgtt
      121 caccggatac gcgacgactg gatatcgatg agctgacagt ttgccattac ttacaaaata
      181 ttcgtcagct accacgcaat ttagggttac atagcaaaga ccgtctatta attaaccagt
      241 caccccccat gccgctggtg acggcgattt ttgatagctt caatgaatcc ggggtaaatt
      301 caccgatact gagcaatatg ctctaccttt cctgtttatc gatgttttct cataagaaag
      361 aactgatccc cttacttttc aatagcatca gcactgtttc aggaaaagtt gaacgcctta
      421 ttagctttga tatcgccaaa cgttggtatc tgcgcgatat cgcggaaaga atgtatacca
      481 gcgagagtct cataaaaaaa aagttgcagg atgaaaatac ctgtttcagt aaaatattac
      541 tcgcttccag gatgtcgatg gccagacgat tactcgagtt acgtcaaatt cctctgcata
      601 ctattgcaga aaaatgtggc tatagcagta cgtcgtactt tataaacaca tttcgacaat
      661 attatggtgt aacgccacat cagtttgcgc aacattcgcc aggtaccttt tcctgacata
      721 ttttgcattt gaatattggt caggatctca cacctgcttc atgtgaaact cttccctgat
      781 gatttctgcc gggctaccgg ctagttctct ttcgcagttt gccaggcggt ataggtattt
      841 tcaggaggaa aagactcggc atgtttggga ttattaagct gacaattcat acgattacgg
      901 gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa tggctggagt ggtttctact
      961 ttcagtgctg tgtactaagc ctggtattcc tgacggttag ctggttgttg agtggtgaat
     1021 ggttagcagg aaagagtaag gctgaacctt cacgttcaac cttactctca tttacacgtt
     1081 acgctttttt aaagcgggca aagagatgtt ccactacaac gaaaaagacc ggaacgaagt
     1141 aaattgccag cactgttgcg gcaaacatcc cgcccatcac gccggtacct accgcgtttt
     1201 gcgcgccaga accggcacca tgactgataa ccagcggcag cacgccgaga ataaaggcga
     1261 gagaggtcat caggattggg cgtaaacgca tccgcgccgc ttcgatgatt gcctctatcg
     1321 gcgttttccc ttctttctgc atcatctcaa cggcaaattc gacaatcagg atggcgttct
     1381 tggcggaaag cccgatggtg gtcagcaaac caacctggaa gtagacgtca ttacttaagc
     1441 cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg
     1501 agaacggaat tgaccagctc tcatagagtg cggcgagggc gaggaacacc acgaccagtg
     1561 aaatggcata cagtgcagga gcctggtttg aggataacgc ttcctgatac gacagtccgg
     1621 tccatgagta gccgacgcct gccggaagtt tagcgaccag gtctgccata aatttcatag
     1681 cgtcaccggt acttttcccg gccgccgctt cacctaaaat ctccattgac gggatgccgt
     1741 tgtagcgttc caatcgcggt gaaccatagg tccattcggt agacgagtag gcagaaagcg
     1801 gtgccatcgt gccagaggcg ttgcgtacat accattggtt gatgttatcc ggcaacatac
     1861 ggaacggcgt gcctgcctgg acatacactt ttttcacccg cccctggttg aggaagtcgt
     1921 tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt gatatcagac agcgccacac
     1981 ccattgcctc agctttcgcg gcgttgacgt tcactttgaa catcggcgta tcttccaggc
     2041 cgttcgggcg taccccggtg acctggtcag gcgattgcgc tgccagtgat aacagctcgt
     2101 ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt gtccagcagt tccatatcaa
     2161 aacctgacgc ggtacccagt tcagccaccg cgggtaagtt gaacgggaag acgacggctt
     2221 tattgatact gcttaacgca atcattgccc gctgaatgat cgcggtaacc gagttttcct
     2281 caccgacacg ttcagaccac ggcttgagac tgataaacgc caggccgttg ttttgcccct
     2341 ggccgctgaa gccaaagccg ccaacggtaa acaccgactg aacattatct ttctcttttg
     2401 taagataata atccgtcacc tgttgcagca ctttcgtggt gttaaccatg gtggcaccgg
     2461 aaggtaactg cgcggtggtc ataaataccc cctgatcctc ttctggtaag aaagaggtcg
     2521 gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag caggtagacc accatgtagc
     2581 gaccggtaca acgcaacagc gagcgggtgc tatcggtata gtgctgagtt gatttttcaa
     2641 acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg accgccttcc ggcgcggctt
     2701 tcagaatggt ggcgcacagg gcaggggtca ggctcattgc cacaaatact gaaagcagca
     2761 tggaggagat cagcgtgatg gagaactggc ggtagatctc cccggttgcg ccgctcataa
     2821 aggccatcgg cataaacact gcggaaagaa caacagcaat accgaccagc gcacgttgga
     2881 tctgccccat cgatttatgc gtcgcttcct tcggtggtag cttatcttcc gcaatgacac
     2941 gctcgacgtt ctccaccacc acgatggcgt catccaccag taaccctatc gccagcacca
     3001 tcccgaacat agttaacgtg ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc
     3061 cgagaataac caccggtacg gcaatcgtcg ggatgattgt ggcacggaaa ttctgcaaaa
     3121 acagatacat gaccaggaag acgaggatga tagcctcaac cagtgtttta aaaacttcct
     3181 gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata aaccgtcttc agacttgcgg
     3241 ggaaataggc tgataagcgg ttcagctcct ctttgactgc ccgcgaggta tccagggcgt
     3301 ttgctccggt agccagtttg atggcgatcc cggcagcagg tttgccgtta tagcgcgcca
     3361 cggtggaata atcttccgct ccaagttcga cgcgggcgac atcacgcagc agcacttgcg
     3421 aaccatcttg ctgaactttc aacaggattt tgccaaattc ttccggcgtt tgcagacgcg
     3481 tctgcacaat gatcgaggcg tttagttgct ggtctgccgc ctgtggcatg ccacccagtt
     3541 gaccaccgga aatctggttg ttttgcacct taatctggga aataacatcg gaaggtacca
     3601 gattgtattt attgagtttt tgcgggtcca gccagatacg catggcgtac tcggaaccaa
     3661 agagctgtac gctaccaacg cccgcggtac ggcttagcgg gtctttgata ttagacgcta
     3721 catagtccgc gatatcgtac tggttgaggc tgccgttatc agaaataaac gccgctacca
     3781 tcagaatatt actgctcgac ttatcaacgc taatcccctg ctgctgcacc gcttcaggta
     3841 atgaaggcat agcgagttgc agtttatttt gcacctgaac ctgtgcgata tcaggagatg
     3901 tcccagtctc gaaggtcaga gtgatagagg cattacccgc cgcatcactg gttgaagaca
     3961 tgtacatcag gccatcaagc ccattcatat tttgctcaat cacctgagtg accgagtctt
     4021 ctaccgtttg cgcatcggca cctggatagg tagcgctgac ggtaatggtc ggtggcgcaa
     4081 tctgcggata ctgcgcaacc ggtaagttca tgatcgccag accacctgca agcatcataa
     4141 taatggcaag tacccaggca aaaaccgggc gatcaataaa atagttagcc atgaaagtcc
     4201 ccttaagcct gcaacgttat tgtttcgatt cggtgctggc gttttcctgg ctggaggaaa
     4261 ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc ggaaacgatc acccgatcgc
     4321 cagcctgcaa gccagaggtg acaacccact ggtcgccgat ggctttgctg gcttcaattt
     4381 cgcgtagctg cacaacatcg tctttatcca gaatgagcgc cgttgcttta ccctgggcgt
     4441 tgtgagtgac gccttcctgc ggcaccagta atacattctg gcggctacct tcatccacta
     4501 atgccgtgac gtacatgcca ggcagcaagt caccatttgg gttggggaaa atcgcccgta
     4561 acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga gaatttcagt gtgccggtct
     4621 ggctgtagcg tttaccattt tccagattga gctgtactgg cgtactgccc tgaacctgtt
     4681 tgatttgccc actggcgacc tcttctttca tgcgcaagaa atcctgcacc gactgcgtga
     4741 gatcgacata aatcgggtcc agacgttgta cggtaaccag cgaatctgcc tgattagcgg
     4801 taacgagtgc gccgacggtc accgacgatt tcccgctgac gcccgtaatc ggcgaggtga
     4861 cattcgcgta ttgcagattg atcgtcgcct gttcaacagc cgctttggcg acggtgacat
     4921 tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta atcctgacgg ctaacgtagt
     4981 tggtcttcag caacgatgcc tggcggttaa aggtgatgcg ggcattgctg gcggtagaga
     5041 gcgctttcgc cagcgagcct ttggcggagt ttagctcggc ctgtaaaggt gcaggatcaa
     5101 tctgatacag cgaatcgccc tggttcactt tatcgccttc gataaagttg cgtttaatga
     5161 taataccgcc cacctgggga cgtatctcgg caacttcata aggaacggtt ctaccgggca
     5221 attcgctcaa cacattgacc gaaccggggg agagtgtgac gacaccgacc tcaggcgtca
     5281 tggcggcggc gttttccgcc gatttgtcat cgcaggcggt gagcatcgcg ccgcagaata
     5341 acaacggtat taacagcttt cttcttctgt tcattttagt ccctgaaaat tcttgagaaa
     5401 taacggtttt ttaaattgcg cgcgaggaga gatatgaggg atttacagac cagcggcata
     5461 gtggggttgt cagccagtaa ggtgggatac aggcacagtg atcgacatgg tgaggtcaac
     5521 gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca gcattcgggc aacgaagggt
     5581 tctactggtg gatacacata cctggggaat aaagtcgttt tagcctctcc tttagtaatc
     5641 accgggggta atgaaatcag catctgcatc cctcgtcatg ccagccatca atttcagttg
     5701 cttatgtcct gactaaaaat aagatgtgat acccagggtg acgatgtcgc tcatacgtct
     5761 tagtttcagc ttgtacataa tgttgaattt ctgaatcttt aatgtttgtt gcgaaatatt
     5821 gagcgtcgac gtgatattgc tttcattttt atttttcaga atcagttgca taattttgaa
     5881 ctgattatgt gaaaaacata attgtgaatt cttatggggc aagtgtttac cataagtaat
     5941 ctcaagaaga tttttgatac tttctttgcg gctaacaaaa taaataggcg ctttagcttt
     6001 tagtttactg atgtgataag gtgccaaaac aataattctc tcggcatcta atttctccag
     6061 aaatttaatc gcttcttcat caaggatatg attgtgatag gtatcaatga taaccttgaa
     6121 gggtttattg cctacttttt tgatctctga cagggaattg atttttatca tttcgtgatt
     6181 atctttcaac tgccaaaagc cctgtaaaag aaaagaatct ttcgtcatga gaaaaatcat
     6241 aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc ttttcattat ttacatcctt
     6301 gtccgaatcg ttgttcaata tagtaaacgc ctataagaac tttacccttc gtgaactaat
     6361 acgtctatta acactaatca atacttcaga tttatccatc acgcactcca tatattgatc
     6421 ctaagtaaga ttcctggttg ttatcagctt gtaaatagta aacaaaaagt taacgtttgc
     6481 ggattactgc ccaagaataa gttatcaata accagtttgt gatctctgaa gaatattact
     6541 aaagttaaaa tgtaatccga tttaaatatc gagtctcctt gtttcgactt aagctggcaa
     6601 ttggattgcc agctttcttt taacgcattg aaattacctg agtaaataac gcattcatgt
     6661 cttcaggttt ttatatagcg tctggagaca cggatattta tgcaatgtaa tcaacgccag
     6721 ttgcaaaaaa atgaacccgg attacttatt taaccgccca tagcatcaac gcttacggaa
     6781 ctctttgttc actttcccac agcactttta aaacattaaa cctgactacg gaaaatatca
     6841 gccatgataa tgtttgaaat ggctaatttg ccatggagtg aaaaaaatta gatgaaattc
     6901 agtaggttga aataatcact agcaggtaat tatttcaatg atagtgcgca attgatctac
     6961 aacactccgt agcggagaga gtattaatcg gatcatagtc atatcaagtg actatgatcc
     7021 gggtgacaac cgggataatt attgctgctt aacgaacaaa ctggcgaagc tgaacaggct
     7081 ggcggcgcta aatatcagtt caattcccac cagtgtggaa accagcgtta cagacaccat
     7141 cggcgttgca ccaaggaata tccaggcaat gacgatatcc agcacaccaa taacgagctg
     7201 tagccagctg cctttcattg aacgctgacg ataccaactc atcaggcgaa taacccctgc
     7261 aacacagaac aaaccggcaa taaatgccgc aatggcaaaa atgcccagct ccggtgcgcg
     7321 gatgaagaaa tagccgatca ataaataggc gactgcgacg aggaaaccgg ataataccgg
     7381 ccagaaatta tgactgcggt tgctgaataa cccgacaata agcgcaatgc ccgagcagat
     7441 taataatgca cccactactg tgcttaaaat atcgccagag acgaacggga aactgataca
     7501 cagcaacccg acgataaaca gtagcacggc aataaactgg attgctctgc gatgtttttt
     7561 aagcatctcc agatcaaact tcaaaattgt tgcct
//
LOCUS       DAZOZD010000200         7588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOZD010000200 DAZOZD010000000
VERSION     DAZOZD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.200"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..295
                     /locus_tag="ACHUPB_003438"
     CDS             <1..295
                     /locus_tag="ACHUPB_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0760166.1"
                     /translation="LKAQFEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNE
                     GTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            658..1695
                     /gene="dusA"
                     /locus_tag="ACHUPB_003439"
     CDS             658..1695
                     /gene="dusA"
                     /locus_tag="ACHUPB_003439"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0760167.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1829..2071
                     /gene="pspG"
                     /locus_tag="ACHUPB_003440"
     CDS             1829..2071
                     /gene="pspG"
                     /locus_tag="ACHUPB_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0760168.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2237..3220)
                     /locus_tag="ACHUPB_003441"
     CDS             complement(2237..3220)
                     /locus_tag="ACHUPB_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0760169.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3303..4718
                     /gene="dnaB"
                     /locus_tag="ACHUPB_003442"
     CDS             3303..4718
                     /gene="dnaB"
                     /locus_tag="ACHUPB_003442"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0760170.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4771..5850
                     /gene="alr"
                     /locus_tag="ACHUPB_003443"
     CDS             4771..5850
                     /gene="alr"
                     /locus_tag="ACHUPB_003443"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0760171.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6103..7296
                     /gene="tyrB"
                     /locus_tag="ACHUPB_003444"
     CDS             6103..7296
                     /gene="tyrB"
                     /locus_tag="ACHUPB_003444"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0760172.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1825 a         1827 c         1986 g         1950 t
ORIGIN      
        1 cctgaaagct cagtttgaaa aattatctca acgtgatatg aagaagtacc tgggagagat
       61 gaagcgtctg gctgatgggg gagaaatgaa ttttgatggc tatctggata agatgtcaca
      121 tctggtgaat gaaggaacgc tcgatcctga tattttaagc aaaatgcgag atgctgcacc
      181 acaattaatt agcttcgcga agtcgtttga cccaacctca aaggaagaga ttaaaatact
      241 tacagacact tctaaattaa tttatgattt gttcggggtt aaatcggaga aataatatgt
      301 gaagttcttc gatagtatgg aaggcattat ataaaaggac ccaatattta ttgggttctt
      361 ttttctctat caatgctatt agcagggaga tatatcacca gagtttaatg tgtgattttt
      421 tatttatcgt cgaacctgga ttgtttatca ttggccttaa caaagttaac ggctaataag
      481 attatttcca tcacttcgtg agagcttcat gccttgagag gatctcaatt ttcttttgca
      541 atgagacagg cgcttcctgt tgttatggta tagtaccccg ctattgagcc tcctgaatag
      601 tgatgctgaa taacataacc ccatgatata tcgataaaat aatctctaca tttgaaaatg
      661 cacggtaatt ctgaaatgca aaaaatcaac caaaccagcg caatgcctga aaaaactgac
      721 gttcactgga gtggtcggtt tagcgttgca ccaatgctcg actggacgga cagacattgc
      781 cgctatttct tgcgtctgct ttcccgcaat acgttgctgt ataccgaaat ggtgaccaca
      841 ggggcgatta ttcacggtaa aggtgattac ctggcgtaca gtgaagaaga acatccggta
      901 gcgttgcaac tcggcggtag cgatccggcg gcgctggcac agtgtgcgaa gctggcagaa
      961 gcgcgtggat atgatgagat caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac
     1021 ggcatgtttg gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg cgtgaaagcg
     1081 atgcgcgatg tggtgtcgat tccagtgacg gtgaaaacgc gtattggcat cgatgaccag
     1141 gacagctatg aatttctctg cgatttcatc aacaccgttt ccggcaaagg cgagtgtgag
     1201 atgtttatca tccacgcacg taaagcctgg ctttcggggt taagcccgaa agaaaaccgt
     1261 gaaatcccgc cgctcgatta tccgcgtgtg tatcaactga agcgtgactt tccgcatctg
     1321 acaatgtcga ttaacggtgg tatcaagtcg ctggaagagg ccaaagcaca cctgcaacat
     1381 atggatggcg tgatggtcgg gcgcgaggcg tatcagaatc cgggtattct ggcggcggta
     1441 gaccgggaga tctttggttc ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc
     1501 atgtatccgt acattgagcg tgaactcagc caggggacgt atctcggcca tattacccgg
     1561 catatgttgg gcttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt
     1621 gaaaatgccc ataaagcggg tgcagacatt aatgtgctgg aacacgcgct caaactggtg
     1681 gcggataagc gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt
     1741 cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt gtaatgccag
     1801 caagagattt catatttggg agagcatcat gctggaacta ctttttgtga ttggcttttt
     1861 tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc tggttgtggc
     1921 gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggtt
     1981 actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa aagtgccgaa
     2041 atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg
     2101 ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg cgcctctaac
     2161 agattcatcg tgctgtaccc tacatacagc cgaactataa aaagaaaggg cttcccaggt
     2221 ggaagcccta tttcttttat ggaatcaaca ggctggaacc ttgcgtcgcc cggctttcca
     2281 gaatctcatg cgcacgctgc gcatccttca gcggatattt ctgctgctcg gcgacatcga
     2341 ccttaatcac accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct
     2401 cccgcgtggt gatatagcct tgcagggaag ggcgtgtcac atacaacgag cctttttgat
     2461 tgagaatgcc taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctgaccatta
     2521 agccgcggcg ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc acggaatcgt
     2581 acaccacgcg cactttctta ccgccggtga tctcttttaa ccgctcgacc agatcctctt
     2641 cacgatagtt aataacctgc cacgcgcccg cttttagcgc gctctgcgct ttttgcgcgg
     2701 ttcctacggt gccgataagt ttcgcgccca gggcttttgc ccactggcag gcaattaagc
     2761 caacgccgcc agccgctgcg tggaacagga actgttcatc gggtttaatt tcataggttt
     2821 tgcgcagcag ataataaacc gttaagcctt tcaggaagga tgccgcagct tgctcaaaag
     2881 aaattgccgc aggcagaatc gccgctttat ccgcattaat gttatgcaca gagctgtaag
     2941 cgcctaacgc cgactgcgca tagactacac gatcgcctgc cttaatatgc tttacaccac
     3001 tgccgacttt actcacgatg cctgccgctt cggtacctaa tccgctgggt agtgatggcg
     3061 gcgggtaaag gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt
     3121 cgacctggat ttcattctct gccggatcgg caggagtgaa ctctacggct tgaagtactt
     3181 ccgggccacc gtgcttgtga aattcaattc gtgttgccat gtgtcctcca gaacgtatcg
     3241 tcagggtctg cttcatatgg taaagtttcg acccattctt tatctcggta actccattca
     3301 ctatggcagg aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac
     3361 aagttgccgg gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg
     3421 gtttaatgct ggataacgaa cgctgggacg atgtagccga gcgtgtggtg gcagacgatt
     3481 tttacacccg cccacaccgc catatcttta ctgaaatggc gcgtttgcag gaaagcggta
     3541 gtcctatcga tctgattacc cttgcggaat cgctggaacg ccaggggcaa ctcgatagcg
     3601 tcggtggttt tgcttatctg gcagagctgt caaaaaatac gccaagtgcg gcgaacatca
     3661 gtgcttatgc tgacatcgtg cgtgaacgtg ccgttgtccg tgagatgatc tcggttgcga
     3721 atgagattgc tgaagccggt tttgatccgc aggggcgtac cagcgaagat ctgctggacc
     3781 ttgctgaatc ccgcgtcttt aaaattgccg aaagtcgtgc aaacaaagac gaagggccga
     3841 agaacatcgc cgatgtgctc gacgcaaccg tggcgcgtat tgagcagttg tttcagcagc
     3901 cacacgatgg cgttaccgga gtaaacaccg gttatgacga tctcaacaaa aaaaccgctg
     3961 gcttgcagcc gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat
     4021 ttgcgatgaa cctcgtcgaa aacgcggcga tgttgcagga taaaccagta cttatcttct
     4081 cgctggagat gccttcagaa cagattatga tgcgttctct ggcgtcgctg tcgcgcgttg
     4141 accagactaa aatccgtacc gggcagctcg atgatgaaga ctgggcgcgc atttccggca
     4201 ccatgggtat tttgctcgaa aaacgcaata tctatatcga tgactcctcc ggcctgacgc
     4261 caacggaagt gcgttcccgc gcacgccgta ttgcccgtga acacggcggc atcgggctta
     4321 tcatgatcga ctacctgcaa ctgatgcgcg taccggcgct ttccgataac cgtacgctgg
     4381 aaattgcaga aatctctcgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg
     4441 tggcgctgtc ccagttgaac cgttctctgg aacaacgtgc cgacaaacgc ccggtcaact
     4501 ccgacctgcg tgaatctggc tctatcgagc aggatgcgga cttgattatg tttatctatc
     4561 gtgatgaggt gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta
     4621 aacaacgtaa cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct
     4681 tcgacaacta tgcggggccg cagtacgacg acgaataata attattttat gaattaggta
     4741 attaaagcaa acacttatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc
     4801 cgcgctctgc gacacaacct gcaacgtctt cgtgaactgg cccctgccag taaaatggtt
     4861 gcggtggtga aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc
     4921 gatgctgacg cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga
     4981 atcaccaaac ctgtactgtt actcgaaggc ttttttgatg ccagagatct gccgacgatt
     5041 tctgcgcaac attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag
     5101 gctagcctgg acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg
     5161 ggcgtaaggc cggaacaggc tgaggcgttt tatcatcgcc tgacacagtg caaaaacgtt
     5221 cgtcagccgg tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgtggcgca
     5281 accgagaaac aactcgctat ctttaatacc ttttgcgaag gcaaacctgg tcaacgttcc
     5341 attgccgcgt cgggtggcat tctgctgtgg ccacaatcgc attttgactg ggtgcgcccg
     5401 ggcatcattc tttatggcgt ctcgccgctg gaagatcgct ccatcggtgc cgattttggc
     5461 tgtcagccag tgatgtcact aacctccagc ctgattgccg tgcgtgagca taaagccgga
     5521 gagcctgttg gttatggtgg aacctgggta agcgaacgtg atacccgtct tggcgtagtc
     5581 gcgatgggct atggcgatgg ttatccgcgc gccgctccat ccggtacgcc agtgctggtg
     5641 aacggtcgcg aagtaccgat tgtcggacgc gtggcgatgg atatgatctg cgtagactta
     5701 ggtccacagg cgcaggacaa agccggggat ccggtcattt tatggggcga aggtttgccc
     5761 gtagaacgta tcgctgaaat gacaaaagtg agcgcttacg aacttattac gcgcctgact
     5821 tcaagggtcg cgatgaaata cgtggattaa tcgttctgta atatttgatt gtctgtgccg
     5881 gatgcggcgt gaatgcctta tccggccaat aaaatcctaa aaattcaata agttgatgtt
     5941 ctttcatgct cttataaagg tcgtgcctct ggcggatgtt cgtttgtcat gagtctcact
     6001 ctgttgctaa ttgccgttcg ctcctgaaca tccactcgat cttcgccttc ttccggttta
     6061 ttgtgtttta accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac
     6121 gcctacgctg gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc
     6181 gacaaagtga atttaagtat cggtctgtac tacaacgaag acggaattat tccacaactg
     6241 aaagccgtgg cggaggcgga agcgcgcctg aatgcgcagc cccatggcgc ttcgctttat
     6301 ttaccgatgg aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcg
     6361 gaccatccgg tactgcaaca acagcgcgta gcaaccattc aaacccttgg cggctcaggg
     6421 gcattgaaag tgggcgcgga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc
     6481 agcgatccta cctgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtgagt
     6541 acttacccct ggtatgacga agcgactaac agcgtgcgct ttaatgacct gttggcgacg
     6601 ctgaaaacat tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg
     6661 ggtgccgatc tcactaatga tcagtgggat gcggtgattg aaattctcaa agcccgcgag
     6721 cttatcccat tcctcgatat tgcctatcaa ggatttagtg ccggtatgga agaggatgcc
     6781 tacgctattc gcgccattgc cagcgctgga ttacccgctc tggtgagcaa ttcgttctcg
     6841 aaaattttct ccctttacgg cgagcgcgtc ggcggacttt ctgttctgtg tgaagatgcc
     6901 gaagccgctg gccgcgtact ggggcaattg aaagcaacag ttcgccgcaa ctactccagc
     6961 ccgccgaatt ttggtgcgca ggtggtggct gcagtgctga atgacgaggc attgaaagcc
     7021 agctggctgg cggaagtaga agagatgcgt actcgcattc tggcaatgcg tcaggaattg
     7081 gtgaaggtat taagcacaga gatgccagaa cgcaatttcg attatctgct taatcagcgc
     7141 ggcatgttca gttataccgg tttaagtgcc gctcaggttg accgactacg tgaagaattt
     7201 ggtgtctatc tcatcgccag cggtcgcatg tgtgtcgccg ggttaaatac ggcaaatgta
     7261 caacgtgtgg caaaggcgtt tgctgcggtg atgtaatgca ggaaagcagg ctggagttac
     7321 ccagcctgca gtgaaattaa actgtcgtcg ctttcactat ttctttgtag atgatttttt
     7381 tgatgccgtt gttctacgtg agagataata aaacgttgtc agttctttta ttgttaagtt
     7441 tatcccaatt atctggaatt cctttcccta ttttttcgtg tggagtaata atttctgcac
     7501 atctaaccat tattaaattt aataataagt atcgggtttg ttacgataga atatttctat
     7561 atcctcaaga atatactttg ccttattg
//
LOCUS       DAZOZD010000201         7521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOZD010000201 DAZOZD010000000
VERSION     DAZOZD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.201"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            10..1299
                     /gene="hpaG"
                     /locus_tag="ACHUPB_003445"
     CDS             10..1299
                     /gene="hpaG"
                     /locus_tag="ACHUPB_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0760173.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1296..2762
                     /gene="hpaE"
                     /locus_tag="ACHUPB_003446"
     CDS             1296..2762
                     /gene="hpaE"
                     /locus_tag="ACHUPB_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0760174.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2764..3615
                     /gene="hpaD"
                     /locus_tag="ACHUPB_003447"
     CDS             2764..3615
                     /gene="hpaD"
                     /locus_tag="ACHUPB_003447"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0760175.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3625..4005
                     /locus_tag="ACHUPB_003448"
     CDS             3625..4005
                     /locus_tag="ACHUPB_003448"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0760176.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4116..4919
                     /gene="hpaH"
                     /locus_tag="ACHUPB_003449"
     CDS             4116..4919
                     /gene="hpaH"
                     /locus_tag="ACHUPB_003449"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0760177.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4930..5718
                     /gene="hpaI"
                     /locus_tag="ACHUPB_003450"
     CDS             4930..5718
                     /gene="hpaI"
                     /locus_tag="ACHUPB_003450"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0760178.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5893..7269
                     /gene="hpaX"
                     /locus_tag="ACHUPB_003451"
     CDS             5893..7269
                     /gene="hpaX"
                     /locus_tag="ACHUPB_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0760179.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7279..>7521
                     /locus_tag="ACHUPB_003452"
     CDS             7279..>7521
                     /locus_tag="ACHUPB_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0760180.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1715 a         2117 c         2141 g         1548 t
ORIGIN      
        1 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
       61 gcatggcagg aagcgttcca gcaatccccc tacaaagccc cgcctaaaac tgcggtctgg
      121 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
      181 gaaaaggtac tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc gacgaaagta
      241 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
      301 ccggaagaga gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
      361 attggcgaaa ccgtggctct cagcaatgtc gataatctga ccatctatac cgagatcaac
      421 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc gcagttgctg
      481 agcgccctga gcgaatttgc cacgctgaat ccaggcgacg ccattctgct cggcacgcca
      541 caggcgcgcg tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga aggtttcccg
      601 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcacaagag cttcccaacg
      661 cagccacacc cgcacggcac gctgtttgcc ctcggcctga actacgccga ccacgccagc
      721 gaactggaat ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc aaataccctc
      781 actggcgata accagacctc cgtgcgtcca aacaatattg aatacatgca ctatgaagcg
      841 gagctggtgg tagttattgg caaacaggcg cgtaacgtca gcgaagccga tgccatggat
      901 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gtgactatct ggaaaactac
      961 taccgcccta acctgcgggt aaaaagccgc gacggactga cgccgatgct ttcaaccatc
     1021 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
     1081 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
     1141 tacttaagtg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacacccaaa
     1201 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
     1261 ctggtgaacc gaattgtgag tgaggataca gcgaaatgaa aaaagtaaat cattggatca
     1321 acggcaaaaa tgttgcaggt aacgactact tccagaccac caatccggca acgggtgaag
     1381 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
     1441 aagaggcgtt cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
     1501 gtctgggcga tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa accgcggaca
     1561 ccggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttcc cacaactttg
     1621 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
     1681 tgctcaacta cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg ccgtggaacg
     1741 taccgtttat gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
     1801 tactgaaaat gtcggaactc tccccgctga ccgctgacag cctgggtgag ctggcgctgg
     1861 aagccgatat tccggcaggc gtgctgaacg tggtacaggg ctacggcgca accgcagggg
     1921 atgcgctggt tcgtcatcat gacgtacgtg ccgtgtcgtt caccggcggt acggccaccg
     1981 ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggaactg ggcggtaaat
     2041 cgccggtgct gatttttgaa gatgccgata ttgaacgcgc gctggacgcc gccctgttca
     2101 ccatcttctc gatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcagcaaa
     2161 gcatctaccc ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
     2221 atccgaacga tccgaatacc caggttggcg cgcttattag ccagcagcac tgggaaaaag
     2281 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgctggcg ggcggcccgg
     2341 ataaaccgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaaccgtgc
     2401 tggcagacgt tgataaccgt atgcgagtcg cccaggaaga gattttcggg ccggtcgcct
     2461 gcctgctgcc gtttaaagac gaagccgaag gcttacgtct ggcaaacgac gtggagtacg
     2521 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
     2581 ttgaagctgg catggtgttc gtcaacaccc agaacgtgcg tgacctgcgc cagccatttg
     2641 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
     2701 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
     2761 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatcactct
     2821 gaactgccag ggaaaaacca cggttgccgc cagggcgcga tcgacgggca taaagagatc
     2881 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac ccactggctg
     2941 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
     3001 gagctgccgc attttatccg cgatatgacc tacaactacg agggcaaccc ggagttgggg
     3061 cagcttattg ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca caacattccc
     3121 agcctgaaac tggaatacgg cacgctggtg ccgatgcgct acatgaatga agacaagcac
     3181 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
     3241 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
     3301 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
     3361 tacacccgcg agttcgaccg ccagatggac gagcgcgtgg tgaagttgtg gcgcgaaggc
     3421 cagttcaaag agttctgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
     3481 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
     3541 gtggagttta tcaccgagct attcccaagc tctggcaccg gtcaggttaa cgctgttttc
     3601 ccgcttcccg cgtaaggacg ttttatgccg cactttatcg ttaaatgcag tgacaacatc
     3661 cgcgaagaag ccgacctgcc ggggttgttc gccaaagtga atccgacgct ggcagccacg
     3721 ggtatttttc cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac ctggcagatg
     3781 gccgacgggc agcgtgatta tgccttcgtg catatgacgt tgaaaatcgg cgcaggtcgc
     3841 agcctggaaa gccgccagca ggcgggtgaa atgctgtttg aactgattaa aacgcacttc
     3901 gccgccctga tggagagccg cctgctggcg ttgtcgtttg agattgaaga gctgcatccg
     3961 acgctgaatt ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc agattgcccg
     4021 gtggcgctgc gcttaccggg cctacaaaac cccaaaccgt acaccgtagg ccggataagg
     4081 cgcagccgca tccggcaatg ccacaggata tcgctatgtt cgataaacac acccacaccc
     4141 tgatcgccca gcgtctggat caggcagaaa aacagcgcga acagatccgc gcgatctcgc
     4201 tggattaccc ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa tgggttcgcc
     4261 tgaaaatcgc cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc tcgaaagcga
     4321 tgcaggcaag ctcgcagatc agcgaaccgg attacggcgc gctgctggat gacatgttct
     4381 tccacgatgg cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc
     4441 tggctttcgt gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca
     4501 acgccacgga ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc cacaacatcg
     4561 atccggaaac ccagcgcccg cgcaaagtgt tcgacaccat ttctgataac gccgccaacg
     4621 ccggggtgat cctcggcggt cgtccgatca aaccagacga actggatctg cgttggatct
     4681 ccgccctgat gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct ggcgtgctga
     4741 atcatccggc aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat gacgtacaac
     4801 tggaagccgg gcaaatcatt ctcggtggtt cgttcacccg cccggttccg gcgcgtaagg
     4861 gcgacacctt ccacgtcgat tacggcaaca tgggttccat tagctgccgc tttgtttaag
     4921 gagagaacga tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc gcagatcgga
     4981 ttatggctgg ggctgagtag cagctacagc gcagagttac tggccggagc aggattcgac
     5041 tggttgttga tcgacggtga gcacacgccg aataacgtgc aaaccgtgct cacccagcta
     5101 caggcgattg cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa cgatccggtg
     5161 caaatcaaac aactgctgga cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac
     5221 gctgacgaag cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg tattcgcggt
     5281 gtgggcagtg cgctggcccg cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa
     5341 gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat gaagaactta
     5401 ccgcagattc tggacgtgga aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc
     5461 gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc cattgagcag
     5521 gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc caatgagcaa
     5581 ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc
     5641 ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtgcgcaggc caccgccgtg
     5701 aagcccggcg tgtattaatg ccggatgcgc tgcgcttatc cggcctacac tcgcaccaaa
     5761 ccgtaggccg gataaggcgt ttacgccgca tccggcaaaa agccgtacca aatcgcggac
     5821 cggataaggc gttacgccgc atccgccata aaccttgtcg taccctacaa aaatcccatt
     5881 agaggaagaa aaatgagcga cacctcacct gccataccgg agagtatcga tccggcgaat
     5941 cagcataaag cgctgactgc cggacaacag gcggttatta agaagctatt tcgccgcctg
     6001 agcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa catcggcttt
     6061 gccggactca cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg cctcgctacc
     6121 accctgttct atgccgctta tgtcatcttc ggcattccca gcaacattat gctgagtatt
     6181 gtcggtgcgc ggcgctggat cgccaccatc atggtgctct ggggcatcgc ctctactgcc
     6241 accatgtttg ccactggccc caccagcttg tacgtactgc gtatactggt tggcattacc
     6301 gaagccggct ttctgcctgg tattctgctg tatttaacct tctggtttcc ggcctatttc
     6361 cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc gttgggctcg
     6421 atcgtttccg gctacatttt gtcgctggat ggcgtcatgg cattaaaagg ctggcagtgg
     6481 ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg gttctggctt
     6541 gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg cctgcaagag
     6601 atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag ccaccacgcc
     6661 atgcaacaac gcagtatgtg gcgggagatc ttcactccgg tggtgatgat gtataccctg
     6721 gcgtatttct gcctgaccaa cacacttagt gcgatcagca tctggacacc gcagatcctg
     6781 caaagtttta atcagggcag cagtaatatc accatcggca tgctggccgc cgtaccgcag
     6841 atttgtacca ttctcgggat ggtctactgg agccgtcact cagatcgccg ccaggaacga
     6901 aggcatcaca ccgcccttcc ttatttgttc gctgccgcag gttggttact ggcttcggca
     6961 actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg atcattcagc
     7021 gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc acgagcgatc
     7081 ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc gtttatgatc
     7141 ggctggttga aagatttgac cggcagcttt aacagtggat tgtggtttgt tgccgcgctg
     7201 ctggtgattg gtgcggggat tatctgggcg attccaatgc agtcctcccg tccgcgagcg
     7261 accctgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat cagcaaagag
     7321 tacgatgaaa gcctgggcac ggacgatgtg cattatcagt cgttcgcccg catggcggca
     7381 ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat gcatttcctc
     7441 aatagcggac agattgagct acagcttgac gatcatcgct actcggtgga agcgcccctg
     7501 tttgtcctga cgccgccgtc a
//
LOCUS       DAZOZD010000202         7510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZOZD010000202 DAZOZD010000000
VERSION     DAZOZD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.202"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..283
                     /gene="rrrD"
                     /locus_tag="ACHUPB_003453"
     CDS             <1..283
                     /gene="rrrD"
                     /locus_tag="ACHUPB_003453"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0760181.1"
                     /translation="ALLNKDLATVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRT
                     STLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            280..750
                     /locus_tag="ACHUPB_003454"
     CDS             280..750
                     /locus_tag="ACHUPB_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0760182.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(839..1351)
                     /locus_tag="ACHUPB_003455"
     CDS             complement(839..1351)
                     /locus_tag="ACHUPB_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0760183.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1546..2310
                     /locus_tag="ACHUPB_003456"
     CDS             1546..2310
                     /locus_tag="ACHUPB_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760184.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2261..3664
                     /locus_tag="ACHUPB_003457"
     CDS             2261..3664
                     /locus_tag="ACHUPB_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0760185.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3664..5130
                     /locus_tag="ACHUPB_003458"
                     /pseudo
     CDS             3664..5130
                     /locus_tag="ACHUPB_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5021..5764
                     /locus_tag="ACHUPB_003459"
     CDS             5021..5764
                     /locus_tag="ACHUPB_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0760186.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5768..6988
                     /locus_tag="ACHUPB_003460"
     CDS             5768..6988
                     /locus_tag="ACHUPB_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0760187.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6992..7498
                     /locus_tag="ACHUPB_003461"
     CDS             6992..7498
                     /locus_tag="ACHUPB_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760188.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1997 a         1800 c         2045 g         1668 t
ORIGIN      
        1 agctctcctg aataaagacc ttgccactgt cgccagacaa attaacccgt acatcaaagt
       61 cgatataccg gaaacaacgc gcggcgctct ttactcgttc gtttacaacg tgggcgctgg
      121 caatttcaga acatcgacgc ttcttcgcaa aataaaccag ggctatatca aaggcgcatg
      181 tgaccagcta tgtcgctgga catatgctgg cggtaagcaa tggaaagggc tgatgacccg
      241 tcgtgagatt gagcgtgaag tctgtttgtg ggggcagcaa tgagcagagt caccgcgatt
      301 atctccgctc tggttatctg catcatcgtc cgcctgtcat gggctgttaa tcattaccgt
      361 gataacgcca ttacctacaa agagcagcgc gacaaaaacg caagagaact gaagctggcg
      421 aacgcaacca ttactgacat gcagcagcgc cagcgtgatg ctgatgcact cgatgctaaa
      481 tacacgaagg agttagctga tgcgaaagct gaaaatgatg ctcttcggcg caagcttgat
      541 aatggtggtc gggtgctcgt caaaggaaaa tgccctgtgc catcctcagc cgaaacctcc
      601 agcgcctccg gcatgggcaa tgatgccacc gtcgaactct ctccagttgc tggacgaaac
      661 gttctcggta tccgggacgg aatcatcagc gaccagacag cattgagaat gcttcaggag
      721 tacatcagga tccaatgcct tggggggtag cggtaatttt actcattatc cttcaaatca
      781 gattctgttg tcagaggaat gggggaagct ggattcgcaa gagcttttta tgggcagttt
      841 agctaaaaaa aggcaacacg aagctaaaac taccaacata aagtgctgac cagctagcag
      901 ctgaaatcag actcactgta tacactttca ctggatgaag ttgcaacatt cctgccacca
      961 aaggagcaat ataacgcagc acggcgataa agcgcgaagt gaaaagtatt gacacagagt
     1021 tattttgcaa ctgaaggcga acccgttcta ttgtattgga acggcttgat attatacatg
     1081 caagttgagg gatatgccca attattatac caagatggta attaacgata gtaccgcacc
     1141 atgcgccact catgaccgtg attccagctt cccatggtga aagagtcgtc ttgctgactg
     1201 taattacagt ggccatcata attgatgctg gtggcaatat cgccgaaatg agtaaagtgg
     1261 attttgtgaa cgctattata aaaagaagcc cccataggcg atatggatga agtacaaaat
     1321 agttcatcaa agcatttatc cactccatca atgtctttcc ttctgtgaat ttttctttgc
     1381 tatttaaccg tgatacgact taatttaggc ttaaacacct tacatttaca aggtactcct
     1441 gtggggggcc ctgccacgga gcgtcgcagg agtgagtgat gaggaagaat taaagcaacc
     1501 tattgtgaca ggcagcgata acagtaaatc acaggaagca taatcatggc aaaaccggac
     1561 tgggaggcca tcgaatcggc ataccgggcc ggagtcctta gcctccgtga tataggcgag
     1621 aaatacggcg ttacagaagg ggctatcagg aagagggcca aaaagcttgg ttgggcacgc
     1681 agtggcggta cgcaggtttg caaaaatggt acgcaaaaaa ggaaagtgcg taccagcaga
     1741 aagcctgcca ttactggcct tacacaaaaa agtacgcaac taaaaacaga atctacaccg
     1801 gatacgaaac cgatacgcgg aatgcgtacc gatcccccga ctaacccatt ccaacccggt
     1861 aaccagcagg cattaaaaca cggtggttat gcccgtcgcc ttctgctcaa agatgaggtg
     1921 atagaggacg ctaaagcgtt gacgctcgag gacgaattat ttcgccttcg tgctaacaac
     1981 cttgtcgctg cagagaatat tggtcggtgg ttggtgtcgc tggaagatgc taatggggac
     2041 caggaaagga agatgctgat tgaaaatatc agcgccgccg agaaagcaat gatgcgcaat
     2101 acagttcgta ttgagtccat cgttggcacg cttgcgacgg taggaaaaat atttgctgat
     2161 acagcctacc gcaaggccgc cactgataag gtgtctctgg aggctgatcg tcttcgccgt
     2221 gatgcaggta ttgatgatgg caatggagag cgtgacctca atgacttcta ctctgacatc
     2281 caaaccgacg ctgaatccgg ctttacgtag tttctggact atgcgggcac gtaacaaagt
     2341 gctttatggt ggtcggtcat cgtcaaaatc atgggatgcc gctggcattg ccatatttct
     2401 gtcgaataaa tacaccctgc gtttttgttg tgcccgtcag atccagaata aaatcgaaga
     2461 gtcggtgtat accctgctca aaattcagat agacaggttt ggtctgcggc accgtttccg
     2521 tattctgaac aacaaaatca ttaaccgggt tactggctcg gaatttgttt tttatggatt
     2581 atggcgcaac atcgaagaaa ttaagtcact ggaggggatc gatgtgttgt ggctggaaga
     2641 agcccacgca ctgacggaat accagtggaa aattctggag ccaacgatcc gtaaagaggg
     2701 ttcggaatgc tggttcatat tcaaccccgg acttgttact gatttcgtct ggcgcaactt
     2761 cgttgttgat ccgcccgaag gcactctcat ccgcaaaatt aactatgacg aaaatccgtt
     2821 tctgtctgac accatgctta aggttatcga cgcggcgcga cgccgtgatc cggatggttt
     2881 taaacatgtg tatgagggcg ttccggagtc tgatgatgat gcggcaatca tcaaactgtc
     2941 ttggatagaa gccgcagtgg atgcgcacaa aacgttaaat ttcgaaccca gtggaagaaa
     3001 gcgtattggc tttgacgtgg ctgacagtgg tacagataag tgcgctaacg tttaccgtca
     3061 cggatccgtt gttttctggg ccgacgaatg gaaggccaaa gaagatgaat tactgaagag
     3121 ctgccagcgt acttatcagg cggcgctgga gcgtgaagca gatattgttt acgactctat
     3181 cggtgttggt gcgtctgccg gtgctaaatt ctctgaaatt aacgctgacc ggaagagcga
     3241 gaacgcatac gcgcgacgtg tgaattacca gaggtttaac gccggtgctg gtgtgcatga
     3301 gccagatgac gagtacaacg gcatccccaa caaagacttt ttcgcaaatc ttaaggctca
     3361 ggcatggtgg ctggtggctg accgtttcag aaatacgttt aacgccatta acaacggaga
     3421 acagtatcct gtggatgagc tgatcagcat agattctcgt tgtccgttgc ttgaaaagct
     3481 gaaactggaa ctgacaacac ctcatcgtga tttcgaccgt aacggacgtg tgatggtcga
     3541 aagtaaaaaa gacctcgcaa aacgcgagat accgtcacca aacgttgctg atgcattcat
     3601 tatggccttc gcgccaatcg atacatcgct ggatatctgg gaacagctgg ggagacaggc
     3661 ctgatggcac gaaacaaaca agccctgcgg cgaactgtgc aggccacagc tgatggttat
     3721 gagaatttta ttgcccgcgt agggatgcag acacctaacc agcactcagc atccacctac
     3781 cgggctaatt tcaccagtcg taaccgcatg ctggtggaat ggtcctatcg ttcatcctag
     3841 atcatcggcg aagcagtcga tgctatcccg gatgatatga cccgcaaagg cattcgcatc
     3901 acttcggaaa ttgatgcaaa agatcgtggc attctcgaat cacaactgga tgagttgcaa
     3961 atctgggatg cgctgaatga cgtgctgaaa tggtcgcgcc tctacggcgg cgcggtgggt
     4021 ttcatcatga ttgaggggta ggcaccaatg accccgctgc gacccgaaac catcggtaag
     4081 ggcaagttta aggggattct cccgctcgac cgctggatgg tcgacccggc actgacccgc
     4141 cgcattaaag atatggggcc ggacctgggt aaacctgagt tttacgatgt ggtgaccaca
     4201 gcaacgggaa ttcctgcctg gcgcattcat cacagtcgcc tgattcgctt tgatggcgtc
     4261 acgttgccat ttcagcagaa gatgaccgag aacgaatggg gaatgtcggt tgtagagcgt
     4321 atctgggatc gtcttaccgc gttcgacagc gctactgtcg gcgcggcgca gctggtctac
     4381 aaggcgcatt tgcgtaccta cagcgtggag aagctacgcg agcttatcgc acttggtggt
     4441 cctgcgtatg aagcgttgct gaagaatatc gacctgattc gacagttcca gagtaatgaa
     4501 ggcatgacgc tcatggactc gcgggataag tttgaaaccc atcagtacag cttcagtggt
     4561 ctggatgaca tcctttcgca gtttgtagaa cagattagtg gcgctgttgg tatcccactg
     4621 gtgcggttgt tcggacagtc cccgaaagga ttttctaccg gtgatgcaga ccttgccaac
     4681 tattacgatc gcatcagttc gttgcaggag aggcgtttac gtcttccggt gcggcggata
     4741 ctggacatca tgcatcgttc ggaacttggc aagccgctgc cggacgattt cacgtttgag
     4801 tttaacccgc tctggcaaat gtctgatgtc gatcgctcaa cggtggcgtt aaacaccacc
     4861 aacgcaatca gtacggcgct gggtgatggt ctgatgacac tgaaagccgc tatgactgat
     4921 ttgcgagaaa attctgacgt aaccggcatc ggggcatcca ttaccgacga ggacatcgag
     4981 aatgccgaag atgaagcgcc gcccggcatc ggcgaacctg atgacgaacc gcaggagccg
     5041 tcaggcggaa atccggtatc gaaccagcct acgcaggata gcgagggcgg tcggagacat
     5101 cgtaaatggt cgctacgatg gttcaaatga cagtatcacg gaaattattg cggcgctgga
     5161 gcgttacagt gaaatcatca ccccctgggc gacaaaggtc gcggaaaact ttactgcgga
     5221 cctaacccgg cagaacgaga aagtttggcg gcaacacagc aagaacatca gtcgcgagct
     5281 ccgcaatctt gtggaaagcg ctcctgtggg ccaggtgatg caatccatca tcgccgaaca
     5341 ggtcaagtac atcaaatctc tgcctctcga ggccgcagac agggtgtacg acatccagaa
     5401 caaagcgata gaggctgttg tcaccggtgg tcgggcggag cagtttgcta aagagattgc
     5461 atctaccggc gatgttgcta agtctagggc cgatctgatt gcccgaacgg aactgggaag
     5521 agcaacgggc gcgctggata tggcccgagc gatagctatt ggctcggatg gttatatttg
     5581 gcgtacagcc gatgatggcg atgtccgaga ttcccacgat cacatgaaag gtaaattcgt
     5641 ccgctgggat tcacctccaa ctttggacgg catgaccggc cacgcgggcg agctgccaaa
     5701 ctgccgctgc tataaagaga tcgtgtttgt tcgcgttcca ttcgcaatga aaagggcagc
     5761 ataacccatg aaatactttt ttgagaccag gctcggggaa acccgatacc gcctggctga
     5821 cggctcgttg ctgtgcaaag acgtgccgat aggacgaaca ggtaagcagc tctatggtgc
     5881 tgatgacctg ccaaaactga aacccgataa gttcggtgaa atagtcgtca cgcgttctcc
     5941 tgagcaggta ttccatccgg ccacgcttgc ctcattcgaa gggatgagca tcacgatcct
     6001 gcatcctgaa gatgaaaacg ggaatgtgcg gctggtaaat cccgagaact ggaaagagct
     6061 tgctgtcggg cacctccaga atgtccggcg cgggacgggt gagcagtctg atttgatgct
     6121 ggctgacctt atcgtcaaag acgaaagcgc cattcagctt atcgaagatg gcctgcgcga
     6181 agtgtcgtgc ggctatgacg cggagtacga gcagaccgag ccaggtaaag ccgagcaggt
     6241 cgatattacc ggaaaccatg tggctcttgt ccctaaaggc agagccggaa atcgttgtgc
     6301 aattggagac agagacacaa tggcaaatca aaagaaaaac tggtggaacc gcatgcgtgc
     6361 ggccatcaag acaggagatg ccgacaccat gaacgaactg gtggagtcgg ctcccgcatc
     6421 ggttacagga gatgaggggg atttgccgca gggcgttaat ctcaacatca acctgtcccc
     6481 gcagcaacca ctaccggaca aagcaccaga gatgggcgga gatccaaccg gcgacagtga
     6541 tgatgacctc aaaacattac tgaaagccct gctggctaag ctggaaagaa atgccacggg
     6601 cgataacgat aataagcctg acgataatcc gaccggtgac ggcgaggacg atgaagagga
     6661 aaccacgatt actggtgact cagcctggcg tgccgaagtt atcgttccgg gtatcgatct
     6721 gagccgtaag atgaaaccga ccgcgttcaa acgcgaggtt ctggcttctg ctgacaaaac
     6781 gctggttcgc cagatagtcg gtgatgcgga tatccgcaaa ttaccgaaac aatcggtcga
     6841 catggcgttt aatgccgtgt ctgaggttgc caaagggcga aacacccgcg ccaccaccgg
     6901 cgatgcacag cgcctaaaca tgggcatgac cagtatcgct tccctgaaca aacaaaacgc
     6961 tgaattctgg gcaaaccgta aagggtaaaa aatgaataat gtatttctgt accggatgcc
     7021 tgttggcatt gccggggctg tctctcgccc gcaggactta accgtcgaac cggtggtcct
     7081 taaatccgat aacgccttcg ctgcctatgg cctggctggt aaatacgatg atgacggttt
     7141 tttcgtgccg ctggcagatg gtgataccgc agacaaggtg aaggggatct acgtgcgccc
     7201 ttatccgacc acgtcgcagc cggacatggt tcgccaggtg ggaacaggca agaacttccc
     7261 gggcgacgcc atgaagcgtg gctacgtgac cgttaatctc ggttctgatt ttgatgccag
     7321 cactatcaaa aaaggcgatc cggtatacgt tgtcgtctcc actgatgaat ccatcaaagt
     7381 gccgctgggt ggattcatgt ccacgtcagt cagtggcaaa aacgtggtgc tgaccaacgc
     7441 tgaattcaca ggtgccggtg atgctgacgg caatgcagaa atttcctgga agatttaagg
     7501 aacagacgaa
//
LOCUS       DAZOZD010000203         7487 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOZD010000203 DAZOZD010000000
VERSION     DAZOZD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.203"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1555
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPB_003462"
     CDS             <1..1555
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPB_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0760189.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
     gene            1566..2639
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPB_003463"
     CDS             1566..2639
                     /gene="lpfD-O113"
                     /locus_tag="ACHUPB_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0760190.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2887..3927
                     /gene="pstS"
                     /locus_tag="ACHUPB_003464"
     CDS             2887..3927
                     /gene="pstS"
                     /locus_tag="ACHUPB_003464"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0760191.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            4014..4973
                     /gene="pstC"
                     /locus_tag="ACHUPB_003465"
     CDS             4014..4973
                     /gene="pstC"
                     /locus_tag="ACHUPB_003465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0760192.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4973..5863
                     /gene="pstA"
                     /locus_tag="ACHUPB_003466"
     CDS             4973..5863
                     /gene="pstA"
                     /locus_tag="ACHUPB_003466"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0760193.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5954..6727
                     /gene="pstB"
                     /locus_tag="ACHUPB_003467"
     CDS             5954..6727
                     /gene="pstB"
                     /locus_tag="ACHUPB_003467"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0760194.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6742..7467
                     /gene="phoU"
                     /locus_tag="ACHUPB_003468"
     CDS             6742..7467
                     /gene="phoU"
                     /locus_tag="ACHUPB_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0760195.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1894 a         1740 c         1947 g         1906 t
ORIGIN      
        1 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
       61 agcgattctt aaacgtgaag gtcagacaga tgttgatgtc agcgtgggtg aattgcgcga
      121 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
      181 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
      241 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
      301 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaacgatt
      361 tgacgacaca gacactagct tgcgcttggt tggctatcgt tactccaccg agggctacta
      421 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
      481 ccgacgtagt tgcgtggagg ggacgctaac gcagtcgttg gggagagatt atggcaattt
      541 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
      601 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
      661 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
      721 catgctctct ttatctgttc ctttattggg ctggtggggt aatagttacg ccacctattc
      781 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
      841 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
      901 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
      961 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     1021 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     1081 ggccgccggg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     1141 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     1201 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     1261 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     1321 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     1381 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     1441 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcact
     1501 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     1561 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     1621 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcaccgta
     1681 cgcttgattt ctcgggatat cttattaccg cgaacgaaaa taaggttgga acgacattta
     1741 ataccactgt aactaatgga tcctcatatc ctggacgctg tcattgtgat actggtaatg
     1801 taggagaatt cccctatatc tattatacgt caaaaataaa ccaggcatta acttatgctg
     1861 gggttcactc taatattaat tattatgatc ttaatccgaa ccttgatgtt gggattgcta
     1921 tagatattct tggggttggg tatgtgaatg caccttttga atatcatgct aacaacccgt
     1981 ctggtaatac aaagtacaat tgcaaccgca ttgaaccttt aagtatatcc agtgggggta
     2041 aagcgatagt atatttttat atcaagaaga catttgcagg aaaattgatt attcctgaaa
     2101 caaagatagt gacattgtat ggaacaatta gccgtgacac cccggtggat tactcacagc
     2161 cgatggctga tgtttatatt cggggcgata ttacagctcc gcaaagttgc gaaataaata
     2221 atttacagcc agtttatttt gattttaaag aaataccagc tgcagatttt tcatctgttg
     2281 ttggaagtgc ggtaacaaca cataaaatta ctaaaacagt cactattgag tgtgaaaacc
     2341 tggggatact gaataccgat gatatcagta cgtcttttta tgctaccgaa cccaatacgg
     2401 acaattcaat ggtcgtaact tcaaactcta acgtggggat aaaaatttac gacaaaaata
     2461 ataaggaaat caaagtgaac ggtggtgagt tgccaacaga catgggtaaa tcaacagtgt
     2521 atggtgaaaa atcaggtagt gtaacttttt cagctgctcc tgcgagcctt actggagctc
     2581 gcccagcccc ggggcaattt actgcaacag cgacgataac tgttgagatt gtgcgctaat
     2641 aggtctgaca acgattgttc atcacatcgt tgtcatctca cccatttctt tatcactttt
     2701 aaatgcatca tgacaaaaag atgacatgtg ggattttcat cttcacattt aaaatcagta
     2761 agttatttgt taattccttt ctgtcataaa aatgtcatat tccttacata taactgtcac
     2821 ctgtttgtcc tattttgctt ctcgtagcca acaaacaatg ctttatgaat cctcccagga
     2881 gacattatga aagttatgcg taccaccgtc gcaactgttg tcgccgcgac cttatcgatg
     2941 agcgctttct ctgtgtttgc agaagcaagc ctgacaggtg caggtgcaac cttccctgcg
     3001 ccggtgtatg ccaaatgggc tgacacttac cagaaagaaa tcggtaataa agttaactac
     3061 cagggtatcg gttcttccgg tggcgtaaaa cagattatcg ctaataccgt tgattttggt
     3121 gcctctgact cgccgctgtc tgacgaaaaa ctggctcagg aaggtctgtt ccagttcccg
     3181 accgtgattg gcggcgtggt gctggcggtt aacattccag ggctgaagtc tggcgaactg
     3241 gtgctggatg gtaaaaccct cggcgacatc tacctgggca aaatcaagaa gtgggatgat
     3301 gaaaccatcg ccaaactgaa tccgggtctg aaactgcctt cacaaaacat tgctgtagta
     3361 cgccgcgcag atggctccgg gactaccttc gtcttcacca gctacctggc gaaagtgaac
     3421 gaagagtgga aaaacaacgt tggtactggc tctaccgtaa aatggccgat cggtctgggc
     3481 ggtaaaggta acgacggtat cgccgcgttc gttcagcgtc tgccgggtgc aattggttat
     3541 gttgaatatg cttacgcgaa gcagaacaac ctggcgtaca ccaaactgat ctccgctgat
     3601 ggtaaaccgg ttagtccgac cgaagaaaac ttcgctaatg cagcaaaagg tgcagactgg
     3661 agcaaaacct tcgctcagga tctgaccaac cagaaaggcg aagatgcatg gcctattacc
     3721 tctaccacgt tcattctgat ccacaaagat cagaagaaac cagaacaagg cacagaagtg
     3781 ctgaaattct tcgactgggc gtacaaaacc ggggctaaac aggcgaacga cctggattac
     3841 gccagcctgc cggatagtgt agttgaacag gttcgcgctg cgtggaagac caatattaaa
     3901 gacagtagcg gtaagccgct gtactaataa aactccaggc cgggtacggt gttttacgcc
     3961 gcatccggca ttacaaaatg actttgtaaa cgcgtttaac tgaagagtaa cttatggctg
     4021 caaccaagcc tgcttttaac ccaccgggta aaaagggcga cataattttc agcgtgctgg
     4081 taaaactggc ggcgctgatt gtgctattga tgttgggtgg cattattgtc tctctgatca
     4141 tctcctcctg gccgagcatt cagaaatttg gtctggcttt cctatggacc aaagagtggg
     4201 atgcaccgaa cgatatctac ggggcgctgg tgccgatcta cggtacgttg gtgacttcgt
     4261 ttatcgcgct gctgatcgcc gtcccggtga gtttcggtat cgccctgttc ctgactgagc
     4321 ttgcgcctgg ctggctgaaa cgcccgctgg gtatcgccat tgagctgctg gcagccattc
     4381 caagtatcgt ttacggcatg tggggcctgt ttatctttgc gccgctgttc gccgtttact
     4441 ttcaggagcc ggtcggcaat atcatgtcga atatcccgat tgttggcgcg ctgttctctg
     4501 gccccgcatt tggtatcggt atcctcgcgg caggcgtgat cctcgccatc atgattattc
     4561 cgtacattgc ggcggtaatg cgtgatgtgt tcgaacaaac cccggtgatg atgaaagagt
     4621 cggcctacgg tattggctgc accacctggg aagttatctg gcgtatcgtt cttccgttca
     4681 ccaaaaatgg tgttatcggc ggcatcatgc tggggctggg ccgcgcgctc ggtgaaacca
     4741 tggcggtgac ctttatcatc ggtaacacct accagctcga cagcgcctcg ctgtacatgc
     4801 cgggcaatag tatcacctct gcgttggcga acgaatttgc ggaagcggaa tccggtctgc
     4861 acgttgccgc actgatggaa ctgggcctga tcctgtttgt gattaccttc atcgtcctcg
     4921 ccgcatcgaa gtttatgatt atgcgcctgg ctaagaatga gggggcacgc taatggctat
     4981 ggttgaaatg caaaccactg cggcgctggc tgaatctcgc cgcaaaatgc aggcgcgtcg
     5041 ccgcctcaaa aaccgtattg cgctgacgct ctcgatggcg acgatggcct tcggcctgtt
     5101 ctggctgatc tggattttaa tgtccaccat cactcgcggt atcgacggta tgtcgctggc
     5161 gctgttcact gaaatgacgc cgccgcccaa tacggaaggt ggtggtctgg cgaacgccct
     5221 ggcgggtagc gggctgttaa ttttgtgggc cacggtattc ggtacgccgc tgggcattat
     5281 ggcggggatt tatctggcgg aatatggtcg taaatcctgg ctggcagaag tgattcgctt
     5341 cattaacgac attctgctct ccgtgccgtc gattgtggtt ggtctgtttg tctacaccat
     5401 cgtggtggca cagatggagc acttctccgg ctgggcgggc gtaattgcgc tggcgctgtt
     5461 gcaggtgccg atcgttatcc gcaccaccga gaacatgctg aaactggtgc cggacagcct
     5521 gcgtgaagcg gcttatgcgc tgggtacacc gaagtggaag atgatctccg cgattacgct
     5581 gaaagcgtcg gtgtccggga ttatgaccgg tatcctgctg gcgattgccc gtattgccgg
     5641 tgaaaccgcg ccgctgctgt ttaccgcgct ctccaaccag ttctggagca cggacatgat
     5701 gcagccgatc gccaacctgc cggtgacgat ctttaagttt gcgatgagcc cgtttgcgga
     5761 atggcaacaa ctggcctggg ccggggtatt gatcattacc ctgtgcgtcc tgctgctgaa
     5821 cattctggcg cgcgttgttt ttgcgaagaa taaacacggt taacactttg ccggatgcgg
     5881 cgtgaacgcc ttacccggcc tacggtaagc ctgattagcg aaaagcatca ggtacgatga
     5941 ggaaaagatt gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt
     6001 gaacttctac tacggcaaat tccatgccct gaaaaacatc aacctggata tcgctaaaaa
     6061 ccaggtaacg gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt
     6121 caacaaaatg tttgaactgt atccggagca gcgcgcagaa ggtgaaattc tgcttgatgg
     6181 cgacaacatc ctgaccaact ctcaggatat cgcactgctg cgtgcgaaag tgggcatggt
     6241 gttccagaaa ccgacgccgt tcccgatgtc catctacgac aacatcgctt ttggtgttcg
     6301 tctgtttgag aagctctccc gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa
     6361 agccgcattg tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg
     6421 tggtcagcaa cagcgtctgt gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct
     6481 gctcgacgaa ccgtgttcgg cgctcgaccc catctctacc gggcgtattg aagagctgat
     6541 caccgaactg aagcaggatt acaccgtggt gatcgttacc cacaacatgc agcaggctgc
     6601 gcgttgttcc gaccacacgg cgtttatgta cctgggcgaa ttgattgagt tcagcaacac
     6661 ggacgatctg ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta
     6721 cggttaattc aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag
     6781 ttcaacgccg aactggaaag tatccgcacg caggtgatga ccatgggtgg catggtggag
     6841 cagcagcttt ctgatgcaat caccgcgatg cataaccagg acagcgatct ggcgaagcgc
     6901 gtgatcgaag gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg
     6961 cgcattatcg ccaaacgtca gccgacggcg agcgacctgc gactggttat ggtgatcagt
     7021 aaaaccattg ccgagctgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg
     7081 ctggagaaat tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt
     7141 cataccatcc agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa
     7201 gcggtacgta tttatcgtga agataaaaaa gtcgatcagg aatacgaagg tattgttcgt
     7261 caactgatga cctacatgat ggaagattcg cgtaccattc cgagcgtact tactgcgctg
     7321 ttctgcgcgc gttctatcga acgtattggc gaccgctgcc agaatatttg tgagtttatc
     7381 ttctactacg tgaaggggca ggatttccgt catgtaggcg gtgatgagct ggataaactg
     7441 ctggcgggaa aagatagcga caaataattc accagacaaa tcccaat
//
LOCUS       DAZOZD010000204         7484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOZD010000204 DAZOZD010000000
VERSION     DAZOZD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.204"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            355..597
                     /gene="yiiF"
                     /locus_tag="ACHUPB_003469"
     CDS             355..597
                     /gene="yiiF"
                     /locus_tag="ACHUPB_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0760196.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(927..1856)
                     /gene="fdhE"
                     /locus_tag="ACHUPB_003470"
     CDS             complement(927..1856)
                     /gene="fdhE"
                     /locus_tag="ACHUPB_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0760197.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1853..2488)
                     /gene="fdoI"
                     /locus_tag="ACHUPB_003471"
     CDS             complement(1853..2488)
                     /gene="fdoI"
                     /locus_tag="ACHUPB_003471"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0760198.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2485..3387)
                     /gene="fdoH"
                     /locus_tag="ACHUPB_003472"
     CDS             complement(2485..3387)
                     /gene="fdoH"
                     /locus_tag="ACHUPB_003472"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0760199.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3400..6450)
                     /gene="fdnG"
                     /locus_tag="ACHUPB_003473"
     CDS             complement(3400..6450)
                     /gene="fdnG"
                     /locus_tag="ACHUPB_003473"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5863..5865),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0760200.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6644..7477
                     /gene="fdhD"
                     /locus_tag="ACHUPB_003474"
     CDS             6644..7477
                     /gene="fdhD"
                     /locus_tag="ACHUPB_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0760201.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1699 a         1974 c         1999 g         1812 t
ORIGIN      
        1 aattataacg atgagcaagt aaagaccggt tggtggcagt actgaccggt ctgtgatgaa
       61 agattaattt caccttaaat cctggtgctg ttaggatgct gacgtttttc ggctacacca
      121 ttcagattat gcaaataaat atccctgaat ataatatatt catagcaccc ataactcatc
      181 ctgggcgata actaaaccat ttttgagtaa taaatgaaac aactgcaacg ctcttattat
      241 ttctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa atgagctttc tttaaagatt
      301 cggcaagggt acttttctct ccatagattc aaaaatgata ttggcgagat atttatgaac
      361 tctctggcag gtatagatat gggcagaatt ttactcgatt tatcgaatga ggtgattaag
      421 caacttgatg atctcgaggt gcagcgtaat cttcctcgtg cggacctatt aagggaagca
      481 gtagatcaat acctgataaa tcaatcgcaa acagcaagaa ccagtgttcc tggcatctgg
      541 caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc tgcgcgagga atggtaatgg
      601 gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt tgttcatgcc ggatgcggcg
      661 tgaacgcctt atccggccta caaagtcatg caaattcaat atattgaagt ttattggtag
      721 gcctgataag cgcagcgcat caggcaatca tacgcttgtc atcagtgaca tacccataac
      781 cttatcgatg tgttggaggg aagaacgagg caaacagaca attgcggcat gatcttgaat
      841 gggtaacgcg gaatacgaga gatggtgtcg tcacacccta tgagctgtaa tccgggcgac
      901 ctgcgccacc cggaaaacgt cagagattac tctccttcac ccggaaacag gaacgggtta
      961 atggaactac gggcgtagcc ttcttgctcc attcgcgcgt ccagtaccag agaggcgagg
     1021 tcatctgcca cggcttcaac tttcggttct ttttcctgat aaagaatctt caggtaagtg
     1081 ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt cgtcatccag cgaccagtaa
     1141 tgcagtttgc cgctctgctc gcagttgctg cattttacgc gcactacgtg ccattcggtt
     1201 tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg tgccaatttg caccatgctg
     1261 gacaccggca tagagccaca taccgggcaa tattgacgtt gttcgccgta ttcagcgcgg
     1321 gctttgccgg ggatcagatt ggccatctgc gcccagtaga gcgacagcgc agcccagata
     1381 aacggcgctt tatcgctgct gacggacgag aaatcagagg caaacagcgc gctggccata
     1441 tcttccagct cctgagtcga tgccttctcc agattctcaa tcactgccag cgccgggccg
     1501 ctcatttcag gtttcagctc agcaattagc gccatcagca gcttttgcca gtgcttatca
     1561 cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg cgctggcttc tttaatgcgc
     1621 gcggtcagat ccatctccag cggatggtcg tacagcacca cttcctgggc gtgggcgata
     1681 agcgcagcaa agcgcaggta atcacccagc ggattatttt ctgccagctc gcgcagacgc
     1741 tcggcgcggc ggttgtataa attcttgagc cgagggaaca ataacggcgg aatcatatcc
     1801 gccgtacgtt tctcgctcga acccagctca tcttgcggga ttatgcgaat actcattcag
     1861 ctttcttttc cgttgtcttg cggacctcac ggtaccagcg cgggtgatgt ttcttcgccc
     1921 aggcgctggt aacccatcct tccaccatgg cggtaatcgt gcctttcacc caaagggcgg
     1981 cgtagatatg caccatgata accacaatta acgccactgc ggcaaatgaa tgcagcatta
     2041 acgcgaatcg gatcaccggg attgagaaag caggcgcaaa ataaggacgc cagataatca
     2101 cgccgctcac cagcagcaga accaggaaaa taatcgccgc ccagaaaacg catttctgac
     2161 cgaagttata acgcccggtg tcacctactt cctcgttgac gacgatctta cgaatattct
     2221 tcgcccaaaa gatatcatcc cgattgatta ggttgtgatg ccagtaacgg aaaaacatga
     2281 tgatgaacga ggcaaacata accacgccga caaacgggtg cagaattcgc gccagctgcg
     2341 gtgtgcccat gatttgcatc aaccagttga aggacgggaa caaaaagccc agcccgctca
     2401 ccgccgccag gatgaagcag aaggcggtga tccagtggtt gatacgttcc ggcgcggtgt
     2461 agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg tctttctctt cgtgcagatt
     2521 attctcttcc tcatccgcac ggttcggacc gacacccacg tagtggaaga tactggccgc
     2581 aaaggtagcc gcaaagccaa cagccgcgag cggtttccag atgcctttcc agaatttcac
     2641 ggtttcgctg atttccgggt tctccggcaa gccatgatac agatttggct tgtcagcatg
     2701 gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc ggatcgtaca gacccgcatt
     2761 gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc gccagcgttt tcatcgactc
     2821 tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc acgcaggctg gttcttgccc
     2881 aaccaccacg cggtcaacgc acagcgtaca tttgtagacg cggttgtctt ccgggttgag
     2941 gcgcggaatg tcgaacggac agcccgcaat gcaataaccg cagccaatgc actgctcgga
     3001 ctggaagtcg acgataccgt tggcatactg aataattgca ccttccgccg ggcacgcttt
     3061 caggcagcct ggatcggaac agtgcataca gccgtctttg cggatcagcc attccagttt
     3121 gtcgttctgc tccacttccg agaagcgcat caccgtccac gatttggcgc ttaaatcatt
     3181 ggggttgtcg tacaccccaa tgttattgcc gacggtatcg cgaatgtcgt tccactctga
     3241 acacgccacc tgacaggctt tacagccgat acaggtggtg acgtcgatga gtttcgccac
     3301 ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga ccgttagtcg cggaacgacg
     3361 aatgatatct tgcgattgat aagccatatg tcgtctccgt tacacctttt ccacattcac
     3421 aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca ccaacgaatg gcgttaacgt
     3481 attggcaata aagccttttt tagctacgcc ttcatagccc cagtgaatcg gaataccgat
     3541 ggtatcgata tctttgccgt tcgctttcag cgtgcgaata cgtttggtca ccaccgcttt
     3601 ggctttgata tagccacggt tggaggagac tttcacggta tcgccctggg caatgccaag
     3661 tttattcgcc agcgactccc cgatttccac aaactgctct ggttgcaaaa tcgcgttcaa
     3721 cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga cgataggtgg ttccgacata
     3781 cgggaactta tcggctttac ccaatgcttc ggcgtcgtct ttaaagattc gcgcagccgg
     3841 gttcgagata acgtttggat gcagcgggtt agttcccagc ggcgtttcaa acggctcgta
     3901 gtgttccggg aacggacctt ccgccatctt atcgagggca aacagacgcc ccatgccttc
     3961 ctgctgcatg ataaacggcc cgacgccgct gcccggaggc gctgcgctgt agtccggaat
     4021 atcccagccg gtccacttag tgccgtccca tttcagcaac tgacgcttcg gatcccacgg
     4081 gttaccctgc ggatctgcgg aggcgcggtt atacagaatg cggcggttaa gcggccatgc
     4141 ccatgcccag cccagcgtgt taccgaggcc agacggatcg gcgttatcac gacgcgccat
     4201 ctggttgcct tccggcgtcc agctaccggc gaaaatccag cagccacagg aggttgtacc
     4261 gtcatcgcgc aattgggcga acgagctaag ttgttggcct ttcttgacga taaccgcacc
     4321 ggttgccgga tcggtaatat cggccagcgc cttaccgttg ctctccatcg ccacttcttc
     4381 cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg ttcagcacct gatccgggtt
     4441 tgcgccaccc tgttcggcat acatcttgcg caagcgcagg aagataccgg agaggatctc
     4501 gccatcagtc agcgcaatcc ctggggcgtc cgcacctttc cagtgccact gcaaccagcg
     4561 acctgagtta acgattgaac cgttctcttc tgcgaagcag gtcgatggca gacggaacac
     4621 ttcggtctgg atcttcgacg agtcaacttc gttcagctca ccgtggttct gccagaagtt
     4681 agaggtttca gtgttcagcg ggtcgatggt gacgaggaac ttcagtttcg acagacaacc
     4741 gatcactttg tttttgttcg ggaatgaggc aacagggtta aagccctggc agatatagcc
     4801 attgaccttg ccctctttca tcatctcgaa gtattgcagg acgtcgtagc ctttatccca
     4861 cttcggcaac cagtcaaagc cccagctatt ttccgccgtc gctttatcac caaagaaggc
     4921 cttcatcata gagacgaaga atttcgggta gttgccccag tagtttacct ggccttccag
     4981 cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga tcggtctgct tctcgcttgg
     5041 cagcgtcatg taacctggca ggctctgcga cagcagcccc aggtccgtca gcccctgaat
     5101 attggagtga ccgcgcaggg cgttaacgcc gccgcctgcc atccccatat tgccgagcag
     5161 cagctggatc atcgccatcg tacgaatgtt ttgcgcacca acggagtgtt gcgtccagcc
     5221 gagggcatac aggaacgagg cagttttatc gtgagcactg gtttctgcga tgtattcgca
     5281 gactttcagg aacgcgtctt ttggcgtacc acagatgttt tcaaccacat ctggcgtgta
     5341 gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc ggatgttgca gcgtggtatc
     5401 gcgtttggcg aagccgtttt cgtccagttc gtaagtccag gaggatttat cgtacttgcg
     5461 tttttccgcg tcatagccgg tgaacaggcc atcttcaaag ccgtaatcct cacgcacgat
     5521 caggctggcg ttggtatagg cttcggtgta ttcgcggttg aatttttcat tgttcagcag
     5581 gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg gaacgaatag gggcatagta
     5641 gtcagccacc gccgccgtac gcgtaaagcg aggatcgatc acaatcagct tcgcgccatt
     5701 gtgaattttg gcttccatcg cccagcggaa cccgaccggg tgagcttcag cggcgttacc
     5761 gcccatcacc acgacgaggt tggcgttctt gatgtcgacc cagtggttgg tcatcgcacc
     5821 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacac gcgcctggtt
     5881 gtcgaccgcg agcataccca gcgcgcggga gaatttttgc gttaaatagc cggtttcgtt
     5941 actcgacgcg gaagcacaca gcatcccggt ggagagccag cggttaacag tcacgccttc
     6001 ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc atcagtttgg cgatgcgatc
     6061 aaacgcctct tcccaactga tttgctgcca tttatcagaa cctggcgcac ggtattccgg
     6121 aaacttcaga cggctttcgg agtggatgaa atccaccagg ccagcgcctt tcggacaaag
     6181 tgcaccgcgg ttgaccgggt gatccggatc gccttcgata tggaagatag atgctttggc
     6241 gttttttgct ccgtcaccga ggctgtacat caacagccca cagcctacgg aacaataggt
     6301 gcaggtatta cgggtttcgc gggtgcgcag cagtttatac tgccgggttt ccgcgagtgc
     6361 tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct gccataccgc cagcgcagat
     6421 cttaaagaac tgccttctgc tgacctgcat ggattgctcc ttgtttcgac attgtcacgt
     6481 cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag ttatttttct ttgcggaagg
     6541 ggccgcaaag gtccagaatt ggctcaattt ccctccatcc aggaagggtt tgtaacagaa
     6601 taccataatg ttggtgtgtg tgttcttatc tggttaagag aaagtgaaaa aaacacagcg
     6661 aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt caaattgagt tatggcggcg
     6721 tgatgattta caacacccac ggttagatga ggtcgcggaa gaagttcccg ttgcgctggt
     6781 ctacaacggc atttcgcatg tggtgatgat ggcgtcgccc aaagaccttg agtactttgc
     6841 gctcggtttt tcgctttccg aggggataat agaaagtccg cgcgatatct tcggcatgga
     6901 tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag ctttccagcc gccgctttat
     6961 ggggttgaag gagcgccgcc gggcgctggc gggacgtacg ggctgtggcg tatgcggcgt
     7021 ggagcaactt aatgacatcg gaaaaccggt gcagccgcta ccgttcaccc agacgtttga
     7081 tctcaacaaa ctggatgatg cattacgtca tctcaacgat tttcagccag tggggcaact
     7141 gactggttgt actcacgccg ctgcctggat gttgccatct ggcgaactgg tcggcgggca
     7201 tgaagacgtg ggtcgccatg tggcgctgga caaactgtta ggccgccggt cacaagaagg
     7261 ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt gccagttatg aaatggtgca
     7321 aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg tctgccgcga ccacgcttgc
     7381 tgtagaagtg gctgagcgct gtaatctgac actggtaggt ttttgtaaac cgggtcgggc
     7441 aacggtttat acccatccgc agcgtttgag caattaatta aaat
//
LOCUS       DAZOZD010000205         7462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOZD010000205 DAZOZD010000000
VERSION     DAZOZD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.205"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(35..565)
                     /locus_tag="ACHUPB_003475"
     CDS             complement(35..565)
                     /locus_tag="ACHUPB_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFR0760202.1"
                     /translation="MSEINYQALREVAERAIPAMERLLMLPADDDLLSEQELKDSGVD
                     IDALNAFKFLAGPETVLALLDEINALEETRINDVCRIAELTKQLELAKSKLNEQREYY
                     EGVISDGSKRIAELESDSQAQKLVEAIIVAIENEQERLFDEDYLMDSKECIDVIREEV
                     KRWNDSRAAGIRIKGE"
     gene            complement(552..806)
                     /locus_tag="ACHUPB_003476"
     CDS             complement(552..806)
                     /locus_tag="ACHUPB_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760203.1"
                     /translation="MSNSARLQLGFSPLSKTIMLAKMRDVEGGRMRVGNDPGRDVTNE
                     AAQLVWQLVMAEGGEIVWELDDGSRMVLKAEKQEAASERN"
     gene            complement(803..1345)
                     /locus_tag="ACHUPB_003477"
     CDS             complement(803..1345)
                     /locus_tag="ACHUPB_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760204.1"
                     /translation="MSNKIENPVVLIHKRENHDSYEVAITNGSHDYYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENAALKESERAFDAMCAEEHGDNWVS
                     ELTETPATDAFLAEVRAQGVDAAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGK
                     VEMTEWLVDFAAQLRKGGNQ"
     gene            complement(1484..1864)
                     /locus_tag="ACHUPB_003478"
     CDS             complement(1484..1864)
                     /locus_tag="ACHUPB_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760205.1"
                     /translation="MFGIDAQRIAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMSNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILYGN"
     gene            complement(1864..2568)
                     /locus_tag="ACHUPB_003479"
     CDS             complement(1864..2568)
                     /locus_tag="ACHUPB_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760206.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GDFDESAKTFIDFIAKRWSEQWKDLEKRANEFDRFMDTMDTAKEALAAGTPLDLESLF
                     NSTMASEMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQREEGKTPGERVAELE
                     AVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMAEVNSSHKDGEVR"
     gene            complement(2630..4315)
                     /locus_tag="ACHUPB_003480"
     CDS             complement(2630..4315)
                     /locus_tag="ACHUPB_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFR0760207.1"
                     /translation="MNTALSIMGDASSNTAIDYRQEMNVIHEIVAECEKEISFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRVWLDNVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWRELFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYEIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            complement(4419..5033)
                     /locus_tag="ACHUPB_003481"
     CDS             complement(4419..5033)
                     /locus_tag="ACHUPB_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760208.1"
                     /translation="MFGMSITQSNGNVWMSPEFTPQNLINRGTMSTSKGSVFKTSIPS
                     NKSCFFFIKSSNKANMMFVHEFRDGYNVLRLNQVGGNPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGKMVYHGEMMPLDAEIITITNPQFTINMGYPCAIMPAMVGVYNYRRTPYDRP
                     VYVTMTGATGNQIYNGEWYSSNVTWNVKKLYTNKVLVINTSKYD"
     gene            complement(5039..5320)
                     /locus_tag="ACHUPB_003482"
     CDS             complement(5039..5320)
                     /locus_tag="ACHUPB_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760209.1"
                     /translation="MAYGTGIYNNKGVNVTGFLTPIFFLDRFTTTSGSKTYPNPPPGK
                     SLHAVCSLMPLNNDNHVNIPTPTITINGNTVSWSNLYKGLGSYIYTYWG"
     gene            complement(5375..5944)
                     /locus_tag="ACHUPB_003483"
     CDS             complement(5375..5944)
                     /locus_tag="ACHUPB_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760210.1"
                     /translation="MCEKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKQRADKLQETLDMVIETQGTHDSNKSETI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMVVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            complement(6084..6242)
                     /locus_tag="ACHUPB_003484"
     CDS             complement(6084..6242)
                     /locus_tag="ACHUPB_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0760211.1"
                     /translation="MLERFRLVTVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(6242..6667)
                     /locus_tag="ACHUPB_003485"
     CDS             complement(6242..6667)
                     /locus_tag="ACHUPB_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HFR0760212.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGGYMISYANGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRAQYEASPRRANAQALRELADVAGTDDA
                     VDVLCLCLDIADQDGIDEPEEQALKKIAQALQLSLDAYL"
     gene            complement(6761..6949)
                     /locus_tag="ACHUPB_003486"
     CDS             complement(6761..6949)
                     /locus_tag="ACHUPB_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760213.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(6959..>7462)
                     /locus_tag="ACHUPB_003487"
     CDS             complement(6959..>7462)
                     /locus_tag="ACHUPB_003487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760214.1"
                     /translation="GVHMARQTYFTSVTKRPRSLRQILAELFSGRVMSRLDELETTVR
                     LLNERLDNQASVVANVWAIVASGSSREAKNARPLVKEKNNKDSSNGKFSKKEAETNGL
                     RSHYSFTGDGSRSSRSEPFDAGFIHHHTSVDDNYHHSSRATCHSGWDDGGCDTSSSSS
                     YSGSCCD"
BASE COUNT         1948 a         1819 c         1557 g         2138 t
ORIGIN      
        1 tttctgggta attgcagaat gcgatgtggt catctcactc tcctttgatg cgaatgccag
       61 cggcgcggga atcattccat cgctttactt cttcacgaat tacgtcaatg cattctttcg
      121 aatccattag gtaatcttca tcaaaaagac gttcctgttc gttttctatc gcaacaatga
      181 ttgcttcaac taacttttgt gcctgagaat cactttctaa ctctgctatg cgcttacttc
      241 catccgagat aacgccctcg taatactcac gctgctcgtt gagttttgat tttgccaact
      301 ccagttgttt tgttagttcc gcaatacggc aaacatcgtt gatacgcgtt tcctctaatg
      361 cgttgatctc atccagtagt gccagcacgg tttctggtcc ggccagaaat ttgaaggcgt
      421 tgagggcatc aatatcaaca ccgctatctt taagttcctg ttcacttaac aagtcatcat
      481 cagctggcaa cattaacagg cgttccattg ctggaattgc acgttccgcc acctcacgca
      541 gtgcctggta attaatttcg ctcactggct gcctcctgct tttctgcctt caacaccatg
      601 cgagaaccat catccagctc ccacactatc tcaccacctt cagccatgac cagttgccac
      661 accaattgag cagcctcatt ggtaacatca cgacctggat cattgccaac gcgcatacgt
      721 ccaccttcaa catcgcgcat ttttgccagc atgatagttt ttgatagcgg tgaaaaacca
      781 agctgtagtc gtgctgaatt actcactggt tgcctccttt gcgaagctgg gcagcaaagt
      841 caactaacca ctcagtcatt tcaaccttcc ctaccaggtc tgaaccaggg tgcatacagc
      901 aatcactctg cgccgctttg aaatccttat actcatattc ttgggccacc agattttttg
      961 cagcttctat agcagcatcc accccctgcg cacgtacttc agccagaaaa gcatcggtgg
     1021 ctggagtctc cgttaattcg ctaacccaat tatctccgtg ttcctcggca cacatcgcat
     1081 cgaatgctcg ctcagattct ttcagggctg cattctccgc cgctaatgcg tctctctgcg
     1141 cccgcaactt ctcaatttcg gcagccatgt agtaaccaac catagcgaag acggcaaaag
     1201 agttgtctgc ctcatcaggc gacactgagg ccattagcag accatcgtaa taatcgtgac
     1261 tcccattggt gatcgccacc tcataggagt cgtggttctc acgcttgtga atgagaacta
     1321 cggggttttc gattttgtta ctcatgtgtt tttctcttct ttgctgttac ataagcacta
     1381 ccaagtgctg gtttattttc acaaataaga aaatgtagaa aacaatttat ttaaggccac
     1441 aacttatggc ctttatttat tcatgccgaa ggatttgtag cgtctaattg ccgtataaaa
     1501 tggggtgagg tgcttgccag taggctccac cggtccatgt gaaccccatg cgaataagag
     1561 tgtttattgc ggctttggta gactccgatt cgacactggc tttacgaaac tcttgaacct
     1621 ctttcgccaa cctgtatgta attgtgcagg ggacgattgc agcccagcct ttctcatgac
     1681 tgttaatgac catctggatg tgaccatcca gaataggttg attagacatg ttattgaaaa
     1741 tgtctgcctg tttttgaatg tgaagaaaaa gccttgccag ctccatttgc tcactacgag
     1801 acaagggatt atcaagaggg ctttttgcaa aagcggctat acgctgtgcg tcaatgccaa
     1861 acatcagcgc acctcaccat ctttatggct ggagttaact tccgccatta aatgtctgac
     1921 gtaatcgaca agagagccac ctggtggaat ctggcattcc tcaactaact ggaagtagat
     1981 atccgcagca ttacgtgtat tgctacccgc gcatattctt tcttcccgaa gtgcatgaag
     2041 ctcgttgatc aaacggtcac actctccgtt acgctgatcc acaacagcct caagttctgc
     2101 gacgcgttca cctggcgtct taccttcttc gcgttggatg gtaacgacga aatcgccaat
     2161 tgcagggacg ttgtaatcca gctcaaggta gtttttggca ccgctgcgga cgaactcacc
     2221 cgcgaacatg gtggcgaaca tctcagaggc cattgtgctg ttgaaaagtg actccagatc
     2281 taacggagtc ccggcagcca gagcctcttt tgctgtatcc attgtatcca tgaaccgatc
     2341 aaactcatta gcgcgctttt ccaggtcttt ccattgctcg ctccaacgtt tggcaatgaa
     2401 gtctatgaat gttttcgcgg attcatcaaa atccccctca aacttaacga tgcctttatc
     2461 aataatgatc tggcctaccg catcctctgc ataacctttc aatgtaaact tgaaaggaaa
     2521 atcagaatgg cctgtgacgt taaaatgctc taactggagg ttgttcatat gtttttctct
     2581 tatcgtttag ttattacatt cttacacaaa ttaagtaagt atttacctat catttaacgc
     2641 gtttgaaaac gtatacgctg actgtgatcc ctgtgtcttc aaactcgttg gtaaacgact
     2701 tccctctggc ataaacgtaa ttatccatcg tcatccagtc cagtattggc gcagtgcccg
     2761 gcaatactgc tacaagacgc ccgccgactt tcagatgccc cagcgcagcc agcgtgtgct
     2821 ctctatgacg accaagcgag tacggcgggt tcataacgat tttgtcgaac tgataacctg
     2881 cgttgtcctc agaccacttt atgaagtcgc agcaaatcgt gttcgtatac ccttttccac
     2941 gcaggatatc agcgaagaga ggtgcgattt ctatgcaggt aacatcttcc agatcggcgt
     3001 taatataggc cagaagatcc ccacgtccgg cttgaggctc caacagcttc tcaccaggct
     3061 tcaattcagt ggctctggca acgtactcag caatcaagcg tggggtaggg tagaactggt
     3121 gtgattttgt atccggtatt aaaccggtag ccacaatcgt atttagcgta tggccgatct
     3181 catacgggaa ctgccagtgc tttttctcct gcacgccgcc aatgaagctc agtgtgcgct
     3241 caagctcttc cacctgcgac ttctggagag ctgaatcaga gaagtaccat acgcctttgt
     3301 ctttgctcaa tcgcccgtca cgaagcgcag tgcgaaccgg cacggagatc gtcttctgga
     3361 ttaacccgaa ctgctttggt gcccgtgttt ttggcgcagt tcggcatggc gcggggattg
     3421 cagcaggcat actgtaagcc agcacctcat tcaacttcca ggccacgtca ggatgtattt
     3481 caaagtgaac gttgccgttc ttgaacatct tcacgcgcat cagatttcca tcgacgttca
     3541 tccagtcacc ggtctggcag tcgtttgccc gatacgcagc tgatagcacc tcagcagtgc
     3601 ggttgatggt aataaattct ttgtgcgcga agaaatgaag caagacacgc agatcgtcga
     3661 tgtagtcctc tttgcgatag ttcacgctaa cgctgtcacg ccagaaatca gaaatgcagt
     3721 cagcgattat cagacgctcg ctgaaaccgt ttgtcttatt tgtcttgtgc gcaggactca
     3781 gcgccttaaa caagccatac acgcgctcag agagatattt gtgcctgtca ttcagcaaat
     3841 taagcatcgt tggtatgacc gtttctgctt tgaactccgg tacaccaacg aactctttaa
     3901 ccttcatctg atagccggtt ctgtcagttt tgatgacttc ctgcttgccc tcgataaata
     3961 gctcacgcca ctcgtcgcga cgggaagctg gcatgatcag cagaacgtta gtcatatccg
     4021 taaccttcct ccagtactcg gcccaaatat tctgtttcac ccattccaga tcgacgttat
     4081 ccagccagac tcgattcaaa cgtgagcgat cttcatctgg tcggtggttt agtcgaagca
     4141 ggcgattaat catgttgtgg cgttcatcgc cataaacgaa gtcgtgtacc tgatacatga
     4201 aggagatctc tttctcgcac tcggccacaa tttcgtggat gacgttcatt tcctgtcgat
     4261 agtcgatagc agtgtttgag ctggcatcgc ccatgatgga aagggctgta ttcataattg
     4321 caccattaag aaatgatttg tttaatgatg caattatttc aaaaataaaa aggtgctaaa
     4381 tagaaattaa caggcaatac ttaaaataaa ggcattgact aatcatactt ggatgtattt
     4441 ataaccagga ctttatttgt atatagtttt tttacattcc atgtaacatt acttgaatac
     4501 cattcaccat tatatatttg gtttcccgtt gcgcctgtca ttgttacgta tacaggtctg
     4561 tcgtaaggtg ttcgtctata gttatatact ccaaccattg caggcattat cgcacacggg
     4621 tatcccatat ttatggtaaa ttgaggattt gtaatagtga taatctctgc atcaaggggc
     4681 atcatctcac cgtgatatac cattttacct gcgctattat acatggcaat accatatcct
     4741 gaatgtggta acaccatatc agaaaaagca taaactgtta tcgttccggg gtttcctcca
     4801 acctgattca gtctgagaac attataccca tctctaaatt catgaacgaa catcatattc
     4861 gccttattac tgctttttat aaagaaaaaa catgatttgt tcgacggtat tgaagtctta
     4921 aaaacagaac ctttagacgt ggacattgtc cccctattaa tcaaattttg cggtgtaaat
     4981 tctggactca tccacacatt accatttgac tgagtaattg acatcccgaa cattattgtt
     5041 atccccaata tgtatatata taagatccaa gtcccttgta taaatttgac caacttactg
     5101 tattgccgtt aatagtgata gttggtgtag gtatatttac atggttatcg ttatttaaag
     5161 gcatcaatga gcacacagca tgtaatgatt tgccaggtgg tggatttggg taagtcttag
     5221 agcctgacgt ggttgtaaat cgatcaagaa aaaaaatagg agtaaggaag ccggtaacgt
     5281 taactccctt attattgtag atccctgtac cgtatgccat ttttcatcct tgtttttatc
     5341 aacataatat ggccgatcat gcggccatat cagatcaatt agagctttga aaggctttgt
     5401 gtggctgtgt tgcgaggtta tgtcgatggc ttaactcacg catcatgtct tcaaggcgac
     5461 tctttgtgtc gtcgagttgg tcagccattg ctcctaaaag ctgacgaact accatcggat
     5521 cgtcgctgtt tagtgatggc attttataac ccgcctgaga agacatcaga ttgaatgctg
     5581 acatcaacat cgttagagag gatttaagcc cggcaatttc acgatctttg ctggcaataa
     5641 tcgtctcact tttgttactg tcatgagttc cttgagtctc gatcaccata tccaaagtct
     5701 cttgcaactt atcagctctc tgtttttcag caagataatg aacaccgaaa tgatgtgcta
     5761 aagctacaac ctgagttggc tcttcaaagg tggactcaaa attaagtgct gtaaccactc
     5821 tctcaaaaat ggaaacgtct tctattcctc gaagaatggc taacattttg acaagttcat
     5881 tagcatccat ttcttcgaat agggcgtttt gttcatcagc tattgctttg catttctcgc
     5941 acatatgttt tccttaatgc ttaaacaagt tgttttctta ttggctttat tgtgatgtgg
     6001 cataaaaggg gagcaagcta aacgtaaagg tgcaaactct ctatttcggt caacgaaaca
     6061 caatggccat ttgacccttg tcattaacac gttgctttac ggactagcgg ataagcgaag
     6121 tagatcgcca gaccaatgag gacgccatca gaaatgacag acatcatctt tcccgtaaaa
     6181 tccaccaaca ccgccatcac cagaagagca atgacggtca caagccggaa tctctcaagc
     6241 attagagata agcatccagt gacaactgaa gcgcctgggc gatctttttc agcgcctgct
     6301 cttctggttc atcaatgccg tcttggtcgg caatatcaag gcacagacaa agtacatcta
     6361 ctgcatcatc agtcccggcc acatccgcca gctcacgtaa agcctgggca ttagcacggc
     6421 gcggtgaggc ttcgtattga gcgcggatat tggcgctcat ctgggctatc tcaccagcaa
     6481 aaggagaaaa tgcaggcaga gctgcaattg ttttctcaag aatggaaatt tcttttgcat
     6541 cgcatgtacc gttggcatag gagatcatgt acccacccca cacggtggct tcaaccgcat
     6601 cacggttctc catttttttg acttcgataa ctgctttacg agttttcttt ttgaagaaac
     6661 ctaacatgtg ttttcctttt gttattggtc aaaacaagtt gttttctagt taaataaatt
     6721 gcttacgcaa agacggtcaa tgcatatgca ctaacaaccg tcagaaataa ccaagtaccg
     6781 aaccgactgg aaaaataaaa atcccaacta cacgagccaa ggtcatgcca gcctgaaact
     6841 ggagatcgcc agagcaaaca agttttataa tgttcgatac ccaaccggcg gccatgaggg
     6901 caagaattgc cagccaaatt tttccaaagt gatttgaaaa ccagttcata caaccgcctt
     6961 agtcacagca tgatcctgag taactggatg aacttgaggt atcgcatcca ccgtcatccc
     7021 atccagagtg acaggtcgct ctactggagt ggtggtaatt gtcgtcgacg gaggtgtggt
     7081 gatggatgaa tccggcatca aaaggctctg accggctgga acggctaccg tcgccagtga
     7141 aactataatg agaacgtagg ccattggttt cagcctcttt ctttgaaaat tttccattcg
     7201 aactgtcctt gttgtttttc tccttcacta aaggccgtgc atttttcgct tcgcgtgaag
     7261 aaccagaggc aacaatcgcc cacacgttcg caacaactga cgcttgatta tctaaacgtt
     7321 cattcagcaa ccgaacggta gtctctagtt catcaaggcg tgacataaca cgaccgctaa
     7381 ataattcggc caaaatttga cgtaaagaac gaggacgttt agttacagaa gtgaaatagg
     7441 tttgacgtgc catgtggact cc
//
LOCUS       DAZOZD010000206         7252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOZD010000206 DAZOZD010000000
VERSION     DAZOZD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.207"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHUPB_003488"
     CDS             <1..208
                     /locus_tag="ACHUPB_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760215.1"
                     /translation="LKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQHM
                     QRREEVNINVHRERGEDVEPGDDF"
     gene            208..5478
                     /gene="traI"
                     /locus_tag="ACHUPB_003489"
     CDS             208..5478
                     /gene="traI"
                     /locus_tag="ACHUPB_003489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0760216.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5498..6244
                     /gene="traX"
                     /locus_tag="ACHUPB_003490"
     CDS             5498..6244
                     /gene="traX"
                     /locus_tag="ACHUPB_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR0760217.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6299..6859
                     /locus_tag="ACHUPB_003491"
     CDS             6299..6859
                     /locus_tag="ACHUPB_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0760218.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            6991..7203
                     /locus_tag="ACHUPB_003492"
     CDS             6991..7203
                     /locus_tag="ACHUPB_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0760219.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
BASE COUNT         1648 a         1839 c         2434 g         1331 t
ORIGIN      
        1 gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga tcagtgaatc
       61 cggtgaagtg gtggatatgg ccgcttatga ggcatggcaa caggaaaatc atccggacat
      121 ccagcagcat atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg agcgcgggga
      181 ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa atcggcaggc
      241 agtgccggaa actattacac cgacaaggat aattactatg tgctgggcag catgggagaa
      301 cgctgggccg gtcagggggc tgaacagctt gggctgcagg gcagcgtcga taaggatgtt
      361 tttacccgtc ttctggaggg caggctgccg gacggggcgg atttaagccg catgcaggat
      421 ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa aagtgtctcc
      481 atgatggcca tgctcggggg ggataaacgt cttattgatg cacataacca ggccgtggat
      541 tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga cggacagtca
      601 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accatgacac cagtcgcgat
      661 caggacccac agttacacac gcatgtggtg gtggctaatg tcacgcagca taacggcgag
      721 tggaagacac tgagcagtga caaagtgggg aaaaccggat tttccgagaa cgtgctcgcg
      781 aaccggattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt tgaggcgctg
      841 ggttatgaaa ccgaagtggt cggtaagcac ggtatgtggg agatgccggg cgtaccggtg
      901 gaggcctttt ccagtcgttc acaggctatc agggaggccg tcggtgaaga cgcctcgctg
      961 aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt cgatccggaa
     1021 atcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1081 cgtgacgcgg cggatcagcg tgcggagatc cgcacgcagg cgcccgggcc tgcttcacag
     1141 gacgggccgg atgtgcagca ggcggtgaca caggcgattg ccggattaag tgaacgaaaa
     1201 gtgcagttca cgtatacgga cgtgctggcc aggacggtcg gcatactgcc gccggaaaat
     1261 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1321 cccctcgacc gtgagaaggg gctgttcaca tcaggaattc atgtgctcga tgagctgtcc
     1381 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     1441 agtgtccccc ggacggctgg ttacagcgat gcggtcagtg ttctggcaca ggaccgtccg
     1501 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     1561 ctggtcatga tggcccggga acaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     1621 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     1681 cagctgctgg aaggcatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggtgaa
     1741 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     1801 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat ggccatgaag
     1861 gatgccgggg tgaacacata tcgctggcag ggcggagaac agcgaccggc caccatcatc
     1921 agtgaaccgg accgtaatgt ccgctatgcc cggctggccg gagattttgc ggccagcgtg
     1981 aaagccggag aagagagcgt ggcacaggtc agcggggtac gggaacaggc catactgaca
     2041 caggccattc gcagtgagct gaaaacgcag ggcgtgctcg gacaccctga ggtgaccatg
     2101 actgcactgt caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccga
     2161 ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgttatc
     2221 gaccgggtga cggcgcagag tcacagcctg accctgcggg atgcgcaggg cgaaacgcag
     2281 gtggtgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg
     2341 gtggcagacg gcgagcgact gagggtgaca gggaaaattc ccggactccg cgtctccggc
     2401 ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccgggg
     2461 cgggctgaac cggccaccct gcctgtggct gattcaccgt tcacggcact gaagctggag
     2521 aacggctggg tggaaacgcc cgggcattcc gtcagtgaca gtgcgacggt gtttgcctcc
     2581 gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag cggtcgtgat
     2641 gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaactcgc ccgccatccg
     2701 tcctttacgg tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc
     2761 gctatcagtc tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc
     2821 cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg
     2881 aagtcgtttg ctgcagaagg aaccggtttt actgaactgg gaggggaaat caatgcgcag
     2941 ataaaacgcg gtgacttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg
     3001 gtttcccgtg cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggcaag
     3061 gaggcggtca cgccgctgat ggagagagta cccggcgaac tcatggagac gttaacgtcg
     3121 ggacagcgtg ccgccacccg tatgatactg gaaacgtccg accgtttcac ggtggtacag
     3181 gggtatgccg gtgtgggtaa gaccactcag ttccgggcgg tgatgtcagc cgtgaacatg
     3241 ctgccggaga gtgagcgtcc ccgggttgtg gggctggggc ccacgcaccg tgcggtcggt
     3301 gagatgcgca gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag
     3361 ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgtttct gctcgatgag
     3421 agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc
     3481 ggtcgtgctg tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct
     3541 ttccgtctcc agcagacgcg cagtgctgcc gatgtggcca tcatgaagga gattgtgcgt
     3601 cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggca
     3661 ctgtccgggc ttgagagtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca
     3721 ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag
     3781 cagaaggcga tgctgaaagg cgaggctttc ccggatatcc ccatgacact gtatgaggcc
     3841 attgtccgcg actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg
     3901 cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc
     3961 ggtgagctgg gcaaagagca ggtcatggtg cctgtcctga acacagcgaa tatacgtgac
     4021 ggagaactgc gtcgtctctc cacctgggag aaaaacccgg atgcccttgc cctggtggat
     4081 agtgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac cctgcaggat
     4141 gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg tgtcacactg
     4201 tacaccccgg acaaaatccg ggtggggacc ggcgaccgga tgcgcttcac gaagagtgac
     4261 cgggagcgcg gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt
     4321 gtcacgctgt cggacggaca gcagacccgg gtgattcgcc ccggtcagga gcgggcagag
     4381 caacatattg acctggccta tgccatcact gcccacggtg cgcagggggc gagtgaaacc
     4441 tttgccatcg cgcttgaagg cacggaaggt aaccggaaac agatggccgg ctttgagtca
     4501 gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag
     4561 ggctggacgg atgccattaa caatgccgta cagaaaggaa ctgcccacga tgtgctggag
     4621 ccgaaaccgg accgggaggt catgaatgcg cagcggctgt tcagtacggc acgggaactg
     4681 cgggacgtgg cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt
     4741 ccggcacggt ttattgcacc ggggcgcaaa tatccgcagc cgtatgtggc actgcctgct
     4801 tttgaccgta acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga
     4861 aacgggctgc ggggattcag cggtgaagga cgggtgaaag gcagcgggga tgcgcagttt
     4921 gtggccctgc agggcagccg taacggcgag agcctgctgg ctgataatat gcaggatggt
     4981 gtccggattg cccgtgataa ccccgacagc ggtgtggtgg tgagaatcgc cggtgaaggt
     5041 cgtccgtgga atcccggtgc catcaccggt ggtcgcgtgt ggggggatat cccggacaac
     5101 agcgttcagc cgggagccgg aaatggcgaa ccggtcacgg cagaggtact ggcacagcgg
     5161 caggctgaag aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa
     5221 atggcagaga acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggagatt
     5281 gccggacagg agcgtgaccg gactgccact tctgaacggg aagccgcgct gccggagagt
     5341 gtgctgcgtg aatcacaacg ggagcgggaa gcggtccggg aggttgcccg ggaaaatctg
     5401 ctgcaggagc gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa
     5461 accctgggcg gagactgata caggaagata aacgctgatg acaacggata acacgaacac
     5521 gacacgtaac gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg
     5581 gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca
     5641 catcaatctg atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt
     5701 tccgctgttt gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc
     5761 ggccattaac cggctgtggg gatgggggat tattgcccag ttcgcatatt atctggcagg
     5821 ctttccctgg tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac
     5881 gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc attcttctga tggccctgtg
     5941 ggggcctttg tccggcacca gttacggcat tgccgggctg ctgatgctgg cagtcagcca
     6001 ccggctgtac cgggcggaag acagagcgga acgtctggcg ctggtcgcct gtctgctggc
     6061 tgttattccg gcgcttaacc ttgccaccag tgatgcagcg gcggtggctg gtcttgtgat
     6121 gacggtgctg accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc gtttctggcc
     6181 cggggatttt ttcccggtat tctacgcctg tcatctggct gtgctgggcg ttctggcgct
     6241 gtgacgggtg tggtatcttt gaccgtaaca ggatgattcg tcagaggcag aacacagcat
     6301 gacagagcag aagcgaccgg tactgacact gaagcgaaaa acggaagggg agacgcctgt
     6361 ccgcaggcgg aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca
     6421 gaagctggcc gagaaagcag cccgggaagc ggagctggcg gcaaaaaaag cgcaggccag
     6481 acaggcgctg tccatttatc tgaacctgcc ctcgctggat gaggccgtga acaccctgaa
     6541 accctggtgg ccgggattat ttgacggtga aacgccccgg cttctggcct gcggtatccg
     6601 ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg
     6661 cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc
     6721 ctgccggtat gatacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta
     6781 tgcggcagcg cgtctggata aaatccgccg ccagaacagg ataaaggcag agcttcaggc
     6841 cgtgcttgat gagaaataaa aaaaagcctc catcagagag gaggcaggga aataaggctt
     6901 aaaaggaaat aacttccgta cccgaaagga agccaattca gtagattatt taacaccata
     6961 atactgacgg gttaaagagg agtgataaag atgaatggat tcagaaacag ttcacggaac
     7021 ggtcaggtct ggcgctggca gcgtgccgga agccgggccg ttattctgga ggtcagtgga
     7081 cgctggatgg aagcggcaga agcatggcga cgggctgccg gtgttgcgcc ccggacagac
     7141 tggcagcagt ttgcccgaaa aagggctgaa cactgccacc ggcgcagccg gggaagggtg
     7201 taacgaaaaa agcagccttc attactccag ggattcagaa ggctgaataa tg
//
LOCUS       DAZOZD010000207         7239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOZD010000207 DAZOZD010000000
VERSION     DAZOZD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.208"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..337
                     /locus_tag="ACHUPB_003493"
     CDS             <1..337
                     /locus_tag="ACHUPB_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0760220.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="ACHUPB_003494"
     CDS             337..666
                     /locus_tag="ACHUPB_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0760221.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="ACHUPB_003495"
     CDS             676..1374
                     /locus_tag="ACHUPB_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0760222.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="ACHUPB_003496"
     CDS             1379..2122
                     /locus_tag="ACHUPB_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0760223.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="ACHUPB_003497"
     CDS             2059..2661
                     /locus_tag="ACHUPB_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0760224.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="ACHUPB_003498"
     CDS             2722..6201
                     /locus_tag="ACHUPB_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0760225.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="ACHUPB_003499"
     CDS             6269..6868
                     /locus_tag="ACHUPB_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0760226.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="ACHUPB_003500"
     CDS             6932..>7239
                     /locus_tag="ACHUPB_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0760227.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgc
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAZOZD010000208         7169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOZD010000208 DAZOZD010000000
VERSION     DAZOZD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.209"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHUPB_003501"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHUPB_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHUPB_003502"
     CDS             complement(4538..4999)
                     /locus_tag="ACHUPB_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0760228.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6928)
                     /locus_tag="ACHUPB_003503"
     CDS             complement(5027..6928)
                     /locus_tag="ACHUPB_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0760229.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1334 a         2194 c         1951 g         1690 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cttggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag attgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggggcggttt tcctggtggt
     5581 aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtct acaatcactt
     5641 cctgacccac acgcggtatc gccaggtggc caaaaccggt gcctgcccac gcctgtgcca
     5701 cacggatcca gcacgaactg tcttggtctg ccgggttata acggtcccag ttgaacttca
     5761 cccgcacgcg accatgttcg tcgcagaaga tttcctcgcc tgccggtcct gtcacgacag
     5821 cgctctgcgg gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg
     5881 ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg
     5941 cctgtggctg ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg
     6001 cctgcggatg ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca
     6061 ttccccgcgc ggtttctgca ttgtttcgcc agccgtccat ctgccagcgg gcaaagttct
     6121 gcccgtgagc acccttgaaa cgtccggggt agtcatacac ttcatactgc gtgcgctggt
     6181 agtcctggtg ctggccttcc tgttcaaaac gtccggccca gccggggcgt ttaaaggtgt
     6241 agtctttggt caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc
     6301 agagggtgct cacctcggta cgggtgttcg ggttccatgg gatttcaaaa gattcgggca
     6361 gatggcggac tgtgtcgcac agcaccaggc tctggtcggt acttttgtaa gcatgctcct
     6421 cataaaagaa gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac
     6481 cgtactggac acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccattcgg
     6541 tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt gatgtcctcg ttctggaaga
     6601 tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc atgctgtaca
     6661 gcatctggtt tttgtcgttc tccccgagtt caaaccaggt caccacgcct ttcacccggc
     6721 gctgtacttc atcgccctgc catatcgtca ggtaggccat tttgtccagc acctgcgcaa
     6781 attcaaggga gagaaactgc tggctgacca gggagagatc gagggaaaag agcgaagaga
     6841 gtgactggtt cagatgaaag gaaaccaccg caaaagcgtc cggcggcagg ccgtccactt
     6901 ccagtgtgaa acgtaatccg gttgacatag caacctccag taaaatgaat aatgatccgt
     6961 accgtacaac tgcggataat gtgataagcg ggacgacttc agacgttatt agggaaggaa
     7021 cagaaaatgc tatggttatg tgagaatttt gtttaaatat tactttacag aaacgggtgt
     7081 ctgttcagtc aaaaaaataa tcacaagctc aacctcttgt ttcttggcgt tattcaggac
     7141 aggtacgcat atcacaacga gtaacaaaa
//
LOCUS       DAZOZD010000209         7121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOZD010000209 DAZOZD010000000
VERSION     DAZOZD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.210"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHUPB_003504"
     CDS             <1..435
                     /locus_tag="ACHUPB_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0760230.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHUPB_003505"
     CDS             518..1420
                     /locus_tag="ACHUPB_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR0760231.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHUPB_003506"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHUPB_003506"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0760232.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHUPB_003507"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHUPB_003507"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0760233.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHUPB_003508"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHUPB_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0760234.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHUPB_003509"
     CDS             complement(5330..6037)
                     /locus_tag="ACHUPB_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR0760235.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHUPB_003510"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHUPB_003510"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0760236.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHUPB_003511"
     CDS             complement(6895..7098)
                     /locus_tag="ACHUPB_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0760237.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1853 g         1638 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgtt tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZOZD010000210         7115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOZD010000210 DAZOZD010000000
VERSION     DAZOZD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.211"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            270..1142
                     /gene="rluB"
                     /locus_tag="ACHUPB_003512"
     CDS             270..1142
                     /gene="rluB"
                     /locus_tag="ACHUPB_003512"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0760238.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(1182..1772)
                     /gene="cobO"
                     /locus_tag="ACHUPB_003513"
     CDS             complement(1182..1772)
                     /gene="cobO"
                     /locus_tag="ACHUPB_003513"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0760239.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(1769..2527)
                     /locus_tag="ACHUPB_003514"
     CDS             complement(1769..2527)
                     /locus_tag="ACHUPB_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0760240.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2747..3796
                     /gene="sohB"
                     /locus_tag="ACHUPB_003515"
     CDS             2747..3796
                     /gene="sohB"
                     /locus_tag="ACHUPB_003515"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0760241.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3832..4083)
                     /locus_tag="ACHUPB_003516"
     CDS             complement(3832..4083)
                     /locus_tag="ACHUPB_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0760242.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4463..7060
                     /gene="topA"
                     /locus_tag="ACHUPB_003517"
     CDS             4463..7060
                     /gene="topA"
                     /locus_tag="ACHUPB_003517"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0760243.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1803 a         1696 c         1933 g         1683 t
ORIGIN      
        1 tattccggtg gtggtgctgg tggcggcgcg ggtggcggag gcggtggtgg ctggtaatta
       61 agctgatgtt aatcgaaaca agctttaacc ataaagatag tgtagtatgt tgcgcctcaa
      121 agccaggcca gttaaaacgt cgagtcgttt acttaaggcc tgaagagttc aaacagactt
      181 tactatcagt gagtcagatg taagttctga ctcactgttg cacaaaagca tatcagacgc
      241 ctgggaaggc gacaccaaaa ggaagctcta tgagcgaaaa attacagaaa gtgctggccc
      301 gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg
      361 ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg ctgaaaattc
      421 gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt cgcgttctgg
      481 cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg cgtccgacgg
      541 tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg
      601 ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac cgtttaatgc
      661 atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag gttgacgacg
      721 cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca gcttttaaaa
      781 ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact ctgaccgaag
      841 gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg agccgcctga
      901 tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt ggctggacgg
      961 agctggacct cgcccagact aactatctgc gcgaactggt ggagcttccg ccagaaacca
     1021 gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggctaatcag attcgtcgtg
     1081 cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcgggcgt aataacggtt
     1141 aatcaaagta tgccggacgt catatccggc atttttacag attaataatc aatccctatt
     1201 tgcgccttaa caccggcatc aaacgcatgt ttgatggggc gcagctcact taccgtatcc
     1261 gccagttcaa gaatatcccg atgacagcct cgacccgtga taatcaccgt ctgttgatgt
     1321 ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata cgccaccata
     1381 tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat ccgctttgca
     1441 tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt atcccaggta
     1501 aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt gcgttcgcca
     1561 ttaggccagg tgcctttaat aaactgcacg acgcccactt tttttccgtg accaactgcg
     1621 cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt aaagacgatg
     1681 ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc tttcactcgc
     1741 tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta cggcccggtt
     1801 gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc cagaggtaga
     1861 gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg aaggcgcttg
     1921 cgcgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg cgctgctgat
     1981 attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct gcatatgcac
     2041 cccagttggc tcgtccctga cgcccaacgc ttgatgaagt aaagaccagt gaaccggcgt
     2101 ccgatttgag taataaagga agcagtgcct gggtgagcat aaaggtggca ttaacgttga
     2161 cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa acatcgccga
     2221 gcaatccggc attatgcaaa acaccatcca gacgcggata attaacgaca atgcgctgtg
     2281 ccagttgttg gcaattttcg gacgtgcagg tcagcaaatc gagaataaac cactgtggct
     2341 gacgcccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct tcattacggc
     2401 ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca cgaccaatcc
     2461 catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt tttggctggt
     2521 aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact ttatgacttt
     2581 ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata acaccgcaaa
     2641 atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct taacatgcct
     2701 gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg aattgttgtc
     2761 tgaatatggt ttgtttttgg cgaaaatcgt taccgttgtg ctagcgattg cggcgattgc
     2821 cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa
     2881 caatctcagc gaacagtata aggagatgaa agaagaactg gccgcggcgc tgatggactc
     2941 acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag aagcgaaagc
     3001 agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc gcgtctgggt
     3061 gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg aagagataac
     3121 ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg aaagccctgg
     3181 tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa
     3241 cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca tgatggcctg
     3301 tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg gggtggtggc
     3361 gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg aactgcacac
     3421 cgccgggcag tataagcgca cgctgacctt gctgggtgaa aataccgaag aagggcggga
     3481 gaaattccgc gaagagctga acgaaacgca tcagttattt aaagattttg tgaagcgtat
     3541 gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg gacaacaggc
     3601 ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc ttagcctgat
     3661 ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca ttgaccgatt
     3721 caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc agcggggtca
     3781 aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc tttaatcaac
     3841 cagatgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa acaccgcggt
     3901 gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccgataa ataccagcac
     3961 gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct catgctcacg
     4021 aatgattttg ttggcctctt tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt
     4081 cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg cagacaaata
     4141 taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg ctaataaagt
     4201 tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca atagatttgc
     4261 ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc
     4321 agtcacctga attttcgtga acagagtcac gacaaggggt tgatatccgc agagaacgag
     4381 tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca ttcctggaag
     4441 aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt ccccggcaaa
     4501 agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca gcgtcggtca
     4561 catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact ctacctccac
     4621 caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt
     4681 tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt
     4741 ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga
     4801 ccgcgaaggg gaagccattg catggcacct gcgggaagtg attgggggtg atgatgcgcg
     4861 ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg catttaacaa
     4921 accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg
     4981 cgtggtgggg tatatggttt cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc
     5041 cggtcgtgtg cagtcggtgg cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc
     5101 gttcgtgccg gaagagttct gggaagtcga tgccagcacg accacgccat ctggtgaagc
     5161 gttggcgtta caggtgactc atcagaacga caaaccgttc cgtccggtca acaaagaaca
     5221 aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg aacgtgaaga
     5281 caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc aacaagctgc
     5341 cagcacccgt cttggatttg gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga
     5401 agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa
     5461 tatggttcgt ggttatatca gcgataattt tggtaagaaa tatctgccgg aaagtccgaa
     5521 tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc cttctgacgt
     5581 caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt
     5641 aatctggcgt cagttcgtag cctgccagat gaccccagcg aaatatgact ccacgacgct
     5701 gaccattggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc gttttgatgg
     5761 ctggacaaaa gtgatgcctg cgttgcgtaa aggcgatgaa gatcgcatct taccagcagt
     5821 taataaaggc gatgctctga cgctcgttga acttacacca gcccagcact ttaccaagcc
     5881 gccagcccgt ttcagtgaag catcgctggt taaagagctg gaaaaacgcg gtatcggtcg
     5941 tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctacg tgcgagtaga
     6001 aaatcgtcgt ttctatgcgg aaaaaattgg cgaaatcgtc accgatcgcc ttgaagaaaa
     6061 tttccgcgag ttaatgaact acgacttcac cgcacagatg gaaaacagcc tcgacctggt
     6121 ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg atttcaccca
     6181 gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga accagatggt
     6241 tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac
     6301 cggggtattc cttggctgtt ctggttatgc gctgccgccg aaagagcgtt gcaaaaccac
     6361 cattaacctg gtgccggaaa acgaagtgct gaacgtactg gaaggcgaag acgctgaaac
     6421 caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg acagctatct
     6481 catcgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg acggttacga
     6541 gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa
     6601 atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa
     6661 cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac caccgaaaga
     6721 agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg
     6781 tgacggcgct gcgggtgttt tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg
     6841 tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta
     6901 tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc gctttagccg
     6961 taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg gctggtcagc
     7021 attttatgtt gatggcaaat gggttgaagg aaaaaaataa cctttaattc tgtcaggttt
     7081 ttataaacaa agggtcgcga aagcggccct ttttt
//
LOCUS       DAZOZD010000211         7092 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOZD010000211 DAZOZD010000000
VERSION     DAZOZD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.212"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..4274)
                     /gene="traI"
                     /locus_tag="ACHUPB_003518"
     CDS             complement(<1..4274)
                     /gene="traI"
                     /locus_tag="ACHUPB_003518"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR0760244.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDHFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKL"
     gene            4356..4583
                     /gene="vapB"
                     /locus_tag="ACHUPB_003519"
     CDS             4356..4583
                     /gene="vapB"
                     /locus_tag="ACHUPB_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR0760245.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4583..4981
                     /locus_tag="ACHUPB_003520"
     CDS             4583..4981
                     /locus_tag="ACHUPB_003520"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR0760246.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4990..>7092)
                     /gene="traD"
                     /locus_tag="ACHUPB_003521"
     CDS             complement(4990..>7092)
                     /gene="traD"
                     /locus_tag="ACHUPB_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0760247.1"
                     /translation="RIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYW
                     WCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVA
                     LVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIGDL
                     PIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSID
                     KILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLM
                     RNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAI
                     RYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGL
                     LAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEK
                     AAATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKD
                     MERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMEN
                     RLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNV
                     PAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDTWQQMQRREEV
                     NINVHRERGEDVEPGDDF"
BASE COUNT         1483 a         2191 c         1807 g         1611 t
ORIGIN      
        1 agtttccggt taccttccgt gccttcaagc gcgatggcaa aggtttcact cgccccctgt
       61 gcaccgtggg cggtgatggc ataggccagg tcaatatgtt gctctgcccg ctcctgaccg
      121 gggcgaatca cacgggtctg ctgaccgtca gaaagtgtga cactgtcacc ggaaaccgct
      181 gtcaccgtcc agacgctgtt ggccacataa ccgcgctccc ggtcactctt cgtgaagcgt
      241 atcctgtcac cggtccccac ccggatggtg tccggggtgt acagcgtgac gccttcagcc
      301 accgcctccc ggggcgaaat cagccgcgtg ttaccttccg catcctgcag ggttatcagc
      361 ccgtcatcct tgctgatacc ggcaatccgg tgatacacat tatccaccag ggcaagggca
      421 tccgggttat tctcccaggt ggagagacga cgcagctccc cgtcgcgtat attcgctgtg
      481 ttcaggacag gcaccatgac ctgcacctgc cccagttctc cggccttttc ccgtgcatca
      541 tgaatcatgc tgttcagtac gcgccgatcc tcattcaggt gcgtgacaat cagcgtctgc
      601 tcccgtgctt ccggtgtcct gccggtatag tcgcggacaa tggcttcata cagtgtcatg
      661 gggacatccg gaaaagcctc gcctttcagc atcgccttct gctgcgcttc tgccagtttc
      721 gcttcctggc tgtgactgaa ctccgtcacg gagtgctccg gtgcccatgc gccctccaga
      781 cgtggcacct gagacggttt cacacgctca agcccggaca gcgccctttc cacatcccgg
      841 ttaatcaggc tgtataccgc ctcccgcagt tccggcgtct gacgcacaat ctccttcatg
      901 atgaccacat cggcagcact gcgcgtctgc tggagacgga aaggctgacc aggcgcgatg
      961 gcctgcagct ggtccgtgtc accgctggcc acagcccgac cgccaccgac cgcaatcagg
     1021 gcgtatgccc gtgccatgtc ggtattgccc accattgagc tctcatcgag caggaacagc
     1081 gtgttgctga aatccggcgt ttctccgctg cgctgctgca gctgcgtgtc atgcagaaag
     1141 gacgccagtg tctgcgcatc cacgccggcg ctgcgcatct ccccgaccgc gcggtgcgtg
     1201 ggacccagcc caacgacacg gggacgttca ctctccggca gcatgttcac ggctgacatc
     1261 accgcccgga actgtgtggt cttacccaca ccggcatagc cctgcaccac cgtgaaatgg
     1321 tcggacgttt ccagtatcat acgggtggcg gcgcgctgtc ctgatgtcag tttctccatg
     1381 agttcgccag gtactctctc catcagcggc gtgaccgcct ccttaccttc gagaatatgg
     1441 cgaagaatgc tcttttctgc ctcatacgac gcacgggaaa ccagcaggcc tgtgccatag
     1501 ccttttgcca catccacata cagtaaatca ccccgtttta tctgcgcatt gatttcccct
     1561 cccaggtcag caaaaccggt tccttctgca gcaaacgact tcgcctctgt cagcaggtcc
     1621 accatgctga aggccaggtt tttactttcc agcacaggaa gggccagatg aatggcctgc
     1681 tgtgccggcg tgtgaagccc ggctttctgc agactgatag cggtttccag caatgtctca
     1741 ccggcacgcg cctttatctg ctcagaaacc accgtaaagg agggatggcg ggcaagtttt
     1801 tccgcagtac gggtttcatc cagtgaggaa tacagccgga catcacgacc actgcgggcc
     1861 agaccgttca gggtggcatt gtccattgcc atctgtgtga cggaggcaaa aaccgtcgca
     1921 ctgtcgctga cggaatgccc gggcgtttcc acccagccgt tctccagctt cagtgccgtg
     1981 aacggtgaat cgctcacagg cagggtggcc ggttcagccc gtcccggcac aacaaccgtc
     2041 atcgcatctt cactgacgga tgccacctgc aggcggtcac cgccggagac gcggagtccg
     2101 ggaattttcc ctgtcaccct cagacgctca ccgtctgcca ccggcatttt ttccggccgg
     2161 aacagcgacc agctgctgtc cagggaagaa atacgcacca cctgcgtttc gccctgcgca
     2221 ttccgcaggg tcaggctgtg actctgcgcc gtcacacgtt ctgtcacata gcggtcatga
     2281 ctgcgtgtct ccgggttcca ctgctccatc accattcccg ggcggtacat atcccgcaga
     2341 taacggctcc ggctgtccag ccagaccggt gacagggcgg tcatggttac ctccgggtgt
     2401 ccgagcacgc cctgtgtttt cagctcactg cgaatggcct gtgtcagtat ggcctgttcc
     2461 cgtactccgc tgacctgtgc cacgctctct tctccggctt tcacgctggc cgcaaaatct
     2521 ccggccagcc gggcatagcg gacattacgg tccggttcac tgatgatggt ggccggtcgc
     2581 tgttctcccc cctgccagcg atatgtgttc accccggcat ccttcatggc catcagtgca
     2641 ctgcctgtac cggttcgctg cccgctgtcg gttatcagga cctgtacgtt atgacgtgcg
     2701 gcaccatcca gcagggttaa cgtctctttc agggagagtt tttcgccctg gtcgacgata
     2761 acggtactgc ccggcgtgaa ggccatgcct tccagcaact gacgacgtcc ggttatcagt
     2821 tcaccggaca gccgttcatc ctgcttcagg ttcatctgcg agcgacggtc agcagcgata
     2881 atctgcacct cccgcccctg ctcccgggcc atcatgacca gttcagccac ccgctcacgc
     2941 tgcccggctg caccgccctg cccggacaca atggccagcg acggacggtc ctgtgccaga
     3001 acactgacgg catcgctgta accggccgtc cgggggacac ttttctccgg atgtacggtc
     3061 acccggttct gtttcatgat gtcacgactg agtgcccgga ctgacagctc atcgagcata
     3121 tgaatcccgg acgtgaacag ccccttctca cgatcgaggg ggataagctg ctcacggctg
     3181 atggcctcat cgataccggc gcgtgcccgt tcaatcacac cattttccgg cggcagtatg
     3241 ccaaccgtcc tggccagcag gtccgtatac ataaactgca ctttgcgttc acttaatccg
     3301 gcaatggcct gtgtcaccgc ctgctgcaca tccggcccgt cctgtgaagc gggtccgggt
     3361 gtctgcgtgc gggtatatgc ccgttgttcc gctgcgtcac gatatgcccg gatgtcgaac
     3421 ccggtttcct tcagcgtctg catccactcg gccattctga cttcaggatc gacgtgctgt
     3481 ttggatttac gcgtatccag cgccgccaca tcccgggatt tcagcgaggc gtcttccccg
     3541 acggcctccc ggatagtctg tgagcgtccg gaaaaggcct ccaccggtac acccggcatt
     3601 tcccacatac cgtgcttacc gaccacttca gtttcatagc ccagcgcctc aacctgctct
     3661 ttccgttttt cccggtagag cctgccaaag gcaatctgat tagcgtacac attctcaatg
     3721 aaccccgttt tccccacttt gtcactgctc agtgtcttcc attcgccgtt atgctgcgta
     3781 acattagtca ccaccgcatg cgtgtgtaac tgtggttcct gatcgcgact ggtgtcgtgg
     3841 ttaaacagtg ccatcaccag attaccggtc agcaccgttt ctgactgtcc gtccgtcatc
     3901 acccgtgtgg aggccagtgc ctccacctga cggacagcaa aatccacggc ctggttatgt
     3961 gcttcaataa gacgtttgtc ccccccgagc atggccatca tggagacact tttgggagcg
     4021 gagaaggtca ggtcgtagcc gggacgatgc ctgttactgc catcctgcat gcggcttaaa
     4081 tccgccccgt ccggcagcct gccctccaga aggcgggtaa agacatcctt atcgacactg
     4141 ccctgcagcc ccagttgttc agccccctgg ccggcccagc gttctcccat gctgcccagc
     4201 acatagtaat tatccttgtc ggtataataa ttatcggcac ttccggccga tctgacctgc
     4261 gcgatactca tcataaacgt atatcccttt gacatatccc ggcatcaatc ccacaataga
     4321 tatacacaag acatatccac ataaggaggc aaataatgga aaccaccgta tttctcagca
     4381 accgcagcca ggcggtcaga ctgccaaaag cggttgcatt gccggaaaac gtaaaacgcg
     4441 ttgaagtgat tgctgtcgga cgaaccagaa tcattacgcc agccggagag acatgggacg
     4501 aatggttcga cggacacagc gtcagcgccg attttatgga taacagggaa cagcccggca
     4561 tgcaggaaag ggagtcattc tgatgctgaa gtttatgctc gataccaaca tctgcatttt
     4621 tacgataaag aacaaacccg ccagcgtcag ggagcgtttt aacctgaacc aggggagaat
     4681 gtgtatcagt tcggtcaccc tgatggagct gatatatggt gcagaaaaaa gccagatgcc
     4741 tgaacgtaat ctcgctgtga tcgagggatt tgtttcccgc attgatgttc tggattacga
     4801 cgctgctgca gccacacaca ccggccagat aagagcagaa cttgcccgtc agggacgccc
     4861 tgtcgggcca tttgatcaaa tgatcgcagg tcatgcccgc agtcgggggc tgattattgt
     4921 gactaataac acccgggaat ttgaacgtgt gggcggcctg agaactgaag actggagctg
     4981 acctgttcat cagaaatcat ctcccggctc aacatcctcc ccgcgttccc ggtgcacatt
     5041 gatgttcacc tcttcacgac gctgcatctg ctgccaggtg tccggatgat tttcctgctg
     5101 ccatgcctca taagcggcca tatccaccac ttcaccggat tcactgatcc cgggtggcag
     5161 ttgctgttcc atctcttctt ccggtttcat tttcagctcc tgctcaatcc cccctgccgg
     5221 aacattcaca cctgaatctg acttcttatc gttgatgaca ggagacaccg gctgttgcgg
     5281 ctgttcagcc tgagtcacgt cttctccgga aacaacctcc gggacatccg gttcgaagag
     5341 gctggccatc tgacgacctt ctgcttccct tgcggcaagt acggcactca gacggttctc
     5401 catttccggg ttgatgtcac gcggaataaa ctccggagcg accttcggac gttcctgata
     5461 tttcagtgag agtttgactg ccggatacgg tccgggcagg gtgacatagc aggtcagatc
     5521 cggcagggac tgaatgtcgg aataactgac cagcgtctgg cgctccatat ccttaccggt
     5581 cgataccccg tcacgtaccg gatcagcacc gtaggaatac tgttcgctgg ctttcaggtg
     5641 ctctttctca ccaatttcac cgacagcgaa ctccgcaatc ttatggctgg gagaacggaa
     5701 aaaggcacgg gtgttcatga cgtcaaacag cgtggcagcc gctttctcac cgtagatatc
     5761 ttccagctgg gcataggact ggataccaaa cacataacag ccaccgaact tacgggcttc
     5821 cggcaggatc tccaccaggt ccggcagttt gtgtaatgtg ggtaactcat cacagaagaa
     5881 ccacacacga cgattacggt tttctcccat tgccagcaga ccacgaatgg caatggacag
     5941 ccacatggag atcaccggct tcagggaggc atgagtgtcg gcattcgacg agataaacag
     6001 ccagccgttt ttctgatcct cccggacacc acgcatccag tcacggatgg tgaagggctc
     6061 accgttatgc tcaatcccct gcaggtaacg gatggctttc acatagttgg tcagcacagc
     6121 acggatggaa atcgccgttt tctcaatttt ctcttccacc aggttggccg ccggtgaatt
     6181 acgcaggtag gtacgcagtt tttcaatttt gatggaaagc agtgtgtcca ccagtttgct
     6241 gtagctgcgg ttggggtcat tacgcatcag atacgccgct tccgcaaaaa tggtacgtcc
     6301 tgaaccctgc cagaacgggt cctcttttgt gcccatcggg atcagagtat ttgcggtatt
     6361 atcaaaatcc ggctgtgtca ggcactcctt ccacaaatcc caggcggcac agcgtgcatc
     6421 cagcggattc aggatcttat cgatggaggg atcatagtaa cttttaacaa attcccctga
     6481 acggtcataa atcaccacca tatcaccacg ctgacgggcg tagttggcca gacgacggat
     6541 aacctccgac ttaccggccc cgaccgtgcc gtgcagacag aagttctgga tttcggaatc
     6601 ccggataatc ggcagatcgc caatccggat atcggagtcc ctgccgtctt ttttcagcat
     6661 ccgggcaacg tctttcggat tgtctgtcag ttgacgacca cctgtgactt cattttcgct
     6721 ctgttgttta ccctgacgcc ccagaatcca ggagacaaca aagaaggtaa tcaggcaaat
     6781 aaccagtgcc acaactgtgg ccagaacgaa tgcagaccat aactgctcac cgcaccagat
     6841 catatattta tcatgcagta cctgagcagc gttcatccgg aaggttttgc cgtaatactg
     6901 aatctcatat accggctgag acttgattaa atcccgcatg ccttccagcg tggtacacca
     6961 ccagtaaata cagccattca caaacgtctg ccagcttatt tttacccata aaaccagacc
     7021 aacgagtatc cagaaaaaaa taaacaggca gtaaagcata atattggcga tctggctgaa
     7081 catgcggata cg
//
LOCUS       DAZOZD010000212         7007 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZOZD010000212 DAZOZD010000000
VERSION     DAZOZD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.213"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHUPB_003522"
     CDS             complement(<1..815)
                     /locus_tag="ACHUPB_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0760248.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHUPB_003523"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHUPB_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHUPB_003524"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHUPB_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHUPB_003525"
     CDS             complement(1397..1873)
                     /locus_tag="ACHUPB_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0760249.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUPB_003526"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHUPB_003526"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0760250.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUPB_003527"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHUPB_003527"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0760251.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUPB_003528"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHUPB_003528"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0760252.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUPB_003529"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHUPB_003529"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0760253.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUPB_003530"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="ACHUPB_003530"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0760254.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1704 a         1748 c         1943 g         1612 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aatttta
//
LOCUS       DAZOZD010000213         6848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOZD010000213 DAZOZD010000000
VERSION     DAZOZD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.214"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUPB_003531"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUPB_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0760255.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUPB_003532"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUPB_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR0760256.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUPB_003533"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUPB_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR0760257.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUPB_003534"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUPB_003534"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR0760258.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4057)
                     /gene="casA"
                     /locus_tag="ACHUPB_003535"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             complement(2496..4057)
                     /gene="casA"
                     /locus_tag="ACHUPB_003535"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4155..>6848)
                     /gene="cas3"
                     /locus_tag="ACHUPB_003536"
     CDS             complement(4155..>6848)
                     /gene="cas3"
                     /locus_tag="ACHUPB_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR0760259.1"
                     /translation="KYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVA
                     AMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPEL
                     KIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGH
                     HGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYI
                     SATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINT
                     LFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGL
                     FFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGS
                     EEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKIL
                     LADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQE
                     APFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGK
                     CIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDS
                     SQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGK
                     DERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPH
                     AARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLP
                     EKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEG
                     EAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1660 a         1861 c         1621 g         1706 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt ttattgagca gcagcgggaa atgatgttcg taccaacagc
     2941 gcgctttcat gttgtcgaaa tcatagccaa atccccacag gcccactttt gcctgcttga
     3001 gactgctggc attaaagagt ttcaccacca gcgcgggaag ttccgtattg ttttctgact
     3061 tacccgtttc gataaggcct aaccagtcgc gccagattaa accgcccggt tgtggtttga
     3121 ccgagtaaaa ctcgccgccc tctttaagta gtacacggta aggcgttaag ggatgctgcc
     3181 acatggcata gttagcgccg taatttttgg tcgtcatcag gctcagaagc gcgtcgctct
     3241 gctcaccgca aatatcgcag ttgccgactg tggtggtatt aaaatcaata cgaatacggc
     3301 gaggcattcc ccagtacgcc tggagtttat tgacctgctc atcggttacc accgcaccgg
     3361 ccagttcgct gatgcgcgtc gggccaagcc aggggaaaac cagatcgtca aattttttgg
     3421 gtagcggtaa atcggcttca tcctgcggca tgacattgat ccacagtttg cgccacaagg
     3481 gagtttgttg attaccctga tattcctgta attcaatcag agtggtcatc ggcccaccgc
     3541 cgcgtaaacc ggtgcggtag cctttgccac ctgacggcgc attcaactgt agggagaaca
     3601 gagctaacgc agagcaatgt gggcacaggt attccgttac accacgcttg ataaagtggt
     3661 ctttattaaa cttcgttgtt tgagcgccgg gaatctcagg cagtagcgaa gcgaccggaa
     3721 ctttatctcc ggtgagcgcc tcgaaatcct gtataaatga aggggaatct ggcccaaact
     3781 ggaaagcatg ttctaatgac agcaatgctt cccgtagctt ttcagcttcc agtccgtctt
     3841 cccagatatc atcccaacga cgatgatctt ttggcgcgaa gctggtttgt agtagcccca
     3901 gcaaaaactg ccataccgca ccctggagat ctgcccgcgg cgcagaaata tcgacaacat
     3961 tttcatccgc cagatcgact ggcgccagtt tgcctgtcgt tccgtcttta aaacgaacgg
     4021 gcaaccacgg ggttgtcaga agtgaaaacg agttcatacg catcctctcg taaaaaagac
     4081 catcccaggt cggttgtcca tctttccatc cttttcggtg tatcaactat tcagagagtc
     4141 ctgtcgcaga catctcaacc aaccttgcca atcaatcctt ccgtagccga atacccacaa
     4201 ctttcgtcat cgttgactaa aataacgttt gccatctccg gatcttgtcg ctgttcaatg
     4261 caccacagcc tgaacgcctc tccctccagt aaagaaaact catcccgatg ttttttccac
     4321 cagcttcggc gcactctgac aacgctcatt tcccatgcgt gagcaccggt ggcataaggc
     4381 ttcaccacac cgtcaataca ggtagccagc cacagggaaa cagactcctc agccagacgt
     4441 gtcgacagtt tttctgggag gtaatcgttg atattggcgg catagccggg cctgaagttc
     4501 agcacaaact ttttagccat tgcgcgatca cagtaatatt tgcccacttg ctcctgctcg
     4561 ctgcgggcaa atccttccgg cattaccacg tcctcaccgt agacggattc aataagaagc
     4621 cgggctgcgt gtggcatctg aatagcacct tgctcacgca gcacacgctg cgtcagccag
     4681 attcgcccat gatcgggata gacaaatgca ctgttacgca tggcactgcc aaaccattcg
     4741 tcgccaggag agtcgtccca gacgggagcc agaatcagca attcaggggg ggaacgctcg
     4801 tcctttccgt cacgctttaa ctgaccatta atatcgcgga tatgccgctg taatcgtcct
     4861 gctcgctgaa tcagcaaatc aacaggggcc aggtcggaga tcatttcgtc caggtcacaa
     4921 tcgacactct gctctaatac ctgagtacaa ataaggactt ttccggcacg ctgtgagcta
     4981 tcttctttac caaagcgtgc cagtgtctcc gtttcaattc gctggcgatc gctaaaagca
     5041 aagcggctat gaaaaagtga aaggctggaa gcaggaatga cgccgcgggc aagcagctga
     5101 cgatgaacct taatagcgtc atcgacagaa ttccggatcc aggcgatgca tttcccctga
     5161 cttaccgccg attcgatacg cgcaatacac tcttgttcac tatgaagcca acccacgctg
     5221 acgctacgct caacgtcttt gcgcgtcgct acccggtgtg agttcacatc ggatttcgtg
     5281 acatgcgtca gccaggggta atcatccttt tccaggaacg gagcttcttg caggccctct
     5341 gtaccccgcg caaaggcagc gacgagtttg tcgcgctgct gttgagataa cgtggcagaa
     5401 agcaaaataa cgctgtttcc gccacgcgcc tgccgctcga tcaacccttc aagaatgcac
     5461 gacatgtaag cgtcacaggc gtggatctca tcagccagca ggattttgtt actcagcccc
     5521 agaagccgca gattattatg tttgaacggc atcaccgcca tcatcgcctg atccagcgtg
     5581 ccgacgccaa tttcagccag caacgccttc ttgttactgt cggcaaacca ggccgcacat
     5641 ccctgactga acgtttgttc atccggttct tctgacccga ctaaatcacc agaccagagt
     5701 gattcattaa agcggtccat taatgtgcgg gcactatgtg ccagcaccag gctggggcgg
     5761 gattctggcg aatagaaagc aagccaggtt ttgaccagcc gatcgtacat ggcattggcc
     5821 gttgccattg ttggcagacc aaaaaacagc ccctgagcct tccccgcagc tatcaacctg
     5881 tgcgccagga taagcgccgc ttctgtttta cctgcgccag tcacatcttc cagaataaat
     5941 aactgcggtc cttgctggct gatatccaga tccagcacct tttgctgtaa cggtgtcgga
     6001 ttctcaataa aaggaaacag cgtattaatt ccgttgaaag gcgcgacttt tgcttttaga
     6061 ggaaagacgg ttaaggcgtt ttgagcctga actaaagttt tctgccagta acctttaata
     6121 tccattgggt gtgcgacgcg tggaaaaaat cgcgttgacg aacccgtcca gtctgcgagc
     6181 acgacggttg cagagatata ccaggaaagg tgttttataa gttcaatgcc ctcatcatca
     6241 tcccagaatg cggggatctc tatgagcgga aacagtggtt tgatttcaag gagaaaatct
     6301 cgtgctgcag ctttgtcttc aggcagaaaa ttatccaatt catccatacg atcagggggc
     6361 caaccatgat gccctgtcgt cacgggcatc cacatttcta tcactcgcct aagtttgcgc
     6421 ggagagagcg aagatgaagg aagtaactcc tgacattcac ttaaataatg attccacaac
     6481 cagtagccca gcgttgaatg agagattttt tcgtaatttt ttctggcacc ttcctgaatc
     6541 ttgagttcag ggggcaggta aagttgttga aaagagcggg caaattttcc aatatcgtgc
     6601 cagcacagca accaggcgaa aaattgagcc gcctgttcct tgtcagaaat ccctaattga
     6661 cgaaaataat cagccagccc gaagcaattt cttttaacca ttaaatagcc catcgcggct
     6721 acatccagcg aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt
     6781 cgggtttttc cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa
     6841 ggatattt
//
LOCUS       DAZOZD010000214         6776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOZD010000214 DAZOZD010000000
VERSION     DAZOZD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.215"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHUPB_003537"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHUPB_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR0760260.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2013..5975
                     /gene="putA"
                     /locus_tag="ACHUPB_003538"
     CDS             2013..5975
                     /gene="putA"
                     /locus_tag="ACHUPB_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0760261.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHUPB_003539"
     CDS             complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHUPB_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0760262.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
BASE COUNT         1617 a         1892 c         1784 g         1483 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
      241 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
      601 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
      721 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
      961 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     1861 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     1921 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     1981 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     2041 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     2101 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     2161 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     2221 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     2281 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     2341 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     2401 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccagg
     2461 ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag
     2521 aagcgttgtt gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca
     2581 gcaacggtaa ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta
     2641 cctgggggct gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc
     2701 gctcgctgaa ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata
     2761 tggcgatgcg cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag
     2821 ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag
     2881 ctgcactgac tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg
     2941 ccattggtaa agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac
     3001 tgtcggcgct gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt
     3061 acccgcgtct gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg
     3121 atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg
     3181 agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct
     3241 gcccgttggt gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga
     3301 ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc
     3361 ttgaaggcta tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg
     3421 cgaaaaagct gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc
     3481 atacgctggc agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt
     3541 tccagtgcct gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg
     3601 acggtaaact taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc
     3661 tggcgtatct ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta
     3721 ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa
     3781 aactggcgca acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg
     3841 atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc
     3901 tggcctcgct ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa
     3961 tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga
     4021 aagatattgt gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa
     4081 gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt
     4141 tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc
     4201 gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc
     4261 tccactacta cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag
     4321 ggcctgtggt gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg
     4381 ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga
     4441 ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat
     4501 tgctgccagg tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg
     4561 gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc
     4621 gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga
     4681 tgattgtcga ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt
     4741 tcgacagtgc gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg
     4801 ccgaccacac gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg
     4861 gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc
     4921 gccatattca gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca
     4981 gcgaagatgc ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg
     5041 atgactttgc cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca
     5101 accgtaacca gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc
     5161 ttggtgtcca tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg
     5221 gtaacctgta tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg
     5281 gcgaagggtt gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc
     5341 tggcgaatcg cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc
     5401 cggtcgatgc acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg
     5461 cagcaaatcg tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag
     5521 gaacacaacg attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc
     5581 gtgagcgcgt gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg
     5641 tgctggcggt gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa
     5701 aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg
     5761 ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg
     5821 aagccgttgc tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa
     5881 gcaatatcct tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg
     5941 ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac
     6001 ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca
     6061 accgtttgat tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga
     6121 ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga
     6181 tcaatcggcg cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca
     6241 attaatgcct tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc
     6301 tccatacaga acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg
     6361 atgtactctt ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc
     6421 agccagatat cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag
     6481 taatacagca gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg
     6541 ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc
     6601 gcgcttactg cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct
     6661 cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac
     6721 tctcatcgcg cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccac
//
LOCUS       DAZOZD010000215         6774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOZD010000215 DAZOZD010000000
VERSION     DAZOZD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.216"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..214
                     /locus_tag="ACHUPB_003540"
     CDS             <1..214
                     /locus_tag="ACHUPB_003540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760263.1"
                     /translation="KKTKRQGNTRVNIKNASESFLARCITMHESDARSVSCVLNTERL
                     KTTSFLPLDGKFCTGILDPISHMEFL"
     gene            complement(281..424)
                     /locus_tag="ACHUPB_003541"
     CDS             complement(281..424)
                     /locus_tag="ACHUPB_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR0760264.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(522..851)
                     /locus_tag="ACHUPB_003542"
     CDS             complement(522..851)
                     /locus_tag="ACHUPB_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HFR0760265.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKK
                     GLWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTA
                     FIITLKM"
     gene            complement(841..2511)
                     /locus_tag="ACHUPB_003543"
     CDS             complement(841..2511)
                     /locus_tag="ACHUPB_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR0760266.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALITAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2744..4963)
                     /gene="mobQ"
                     /locus_tag="ACHUPB_003544"
     CDS             complement(2744..4963)
                     /gene="mobQ"
                     /locus_tag="ACHUPB_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR0760267.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADTVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            5142..5408
                     /locus_tag="ACHUPB_003545"
     CDS             5142..5408
                     /locus_tag="ACHUPB_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFR0760268.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5633..6526)
                     /locus_tag="ACHUPB_003546"
     CDS             complement(5633..6526)
                     /locus_tag="ACHUPB_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0760269.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
BASE COUNT         1600 a         1870 c         1563 g         1741 t
ORIGIN      
        1 aaaaaaaaca aaaagacaag gaaacaccag ggtaaacata aaaaatgcgt cagaatcgtt
       61 tttagcgcgt tgcataacta tgcacgaaag cgatgcacga tcggtttcgt gcgttctgaa
      121 caccgaacgg ctcaaaacga caagttttct ccccctggat ggaaagttct gcacggggat
      181 tttagatccc atctcccaca tggaattttt gtgacacttc aaaaaaaagc gcgtcagaat
      241 cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg
      301 tcagttcagc tgaggatgac ggggatacag tcccgccctg aacatcgcgg atatagtttg
      361 cctgacatgc agcaagaata atggctgcaa gaagcaataa aataatccat gttatttttt
      421 tcatactgtt ccttgtatat atggactcag gcattcatcc ataaaaacct gaaatccaaa
      481 ggttgaaatc atccatgaaa cataaaattc tataacacta tttacatctt taacgtgata
      541 atgaaagcag tgtatccatt ctcaatatca tctttaatat tttcaatagc caatctatta
      601 ttttcaggga tttttgattt gtattcctcc ggcatatcat atgtccatga tgctgaacta
      661 tctatcctga atccaggatt cagccaatgc ccaccatcaa tgcccaaaat ccataagcct
      721 tttttcgctg cttcattaca aacttctatt gcagcttttg gtgataactt catagaagca
      781 tttcctttta attcaaaaaa ggcatcacca ctatcatata acaccgtatg ttcaaataat
      841 ttattggtca tttttatttc cccattgaat tctcgaatcg tccacccaac ccggatctgt
      901 cttatcacta atctgagtaa cctcaccagt acgatcattg acaacaacat atttaccagg
      961 agaaccatac actgttgctg gatcatttcg acctaaatag tctggaggtg ttttcttcgg
     1021 actacgctta tcaaaagatg taccagttgc accgttagat acggtatttt tgatatcatc
     1081 ctctgtccat cctctttctg gcatttgtcc cctaattttc tgatctattt ttagacctgg
     1141 tatctttcct ttattttttg ctaactcatc atttagtttg ccttcagcat ctcttttatt
     1201 atcctctttc ttcttcctgc tttccatcgc agtaattaat gcagcatcag catctgcctt
     1261 ctcttttgca gcagcatcaa atgcagcctg cttattattt acatccgtct gcgcccgctg
     1321 agctttaagc ccggccattt gccacattct gtgaccgcca gccattgggt catgtgcaaa
     1381 tcgttcaaat tgttttattt cagctattgc atcagcaaga gttttattcg ctgcatcaag
     1441 ttcgctttta cgcgaattat aaacctgaac agctttagcc tgtcgctcct gatttctggc
     1501 aacatcttca tttgcctgat tcagctctgc acgcgcgcgt tcataatttc gctcagccgc
     1561 ttcaaccgga tgcgtagcat cccattcctg ctgacggcga ttttcttcat cctgacgttg
     1621 ttttacctgg tcagggctaa gaacatcact cactgaaaca tatacggcat tatgaccgct
     1681 gtccttcggg aatcgaataa ctgcatccct ggtattaccc ccctgagtaa atcctgccgg
     1741 gcgaacatcc ttatcagtat tatttgtaac acctgggctt aatgtctgta ctgctggcgt
     1801 actgttatta actgaaatat tcagaacagg tgcacctgga attgatgccg taaaaacacc
     1861 tggacgttcg gtaggttttg catcaaccac cggaacactc atcggaacac ctgaaacaac
     1921 cgaaatattc tgtcgttcgt cttttacatc atcaacaaca cgaacattta cgtttactgt
     1981 tgccttatcg agaggtaatg aactgacagg tgattcagta atatcatctg cgggtaatga
     2041 cgtcacaatc tttgacatca tattggggtc atctttcgct atttgtgatg gcaatacacc
     2101 atataaagcc accccccaaa gaccaaattt aaacggtcct ttcagggcag ccataatatc
     2161 agcaatagct gccgataatg ctcccgctga aatactgacc gccagaccgc cagctcctgg
     2221 agtggaaagt gccggaaaac caaatgccac tggcgcagct actgctgaca gattaccgcc
     2281 tgttcccgag ccaccaccgg aattaccatt ccccccgcca ttaccatgac cggaaccacc
     2341 accccagtga atgccgctac cggaaccacc accccacggg ttattttccg aactccatcc
     2401 ggagccatca gaagcaccac cacctacacc aagcccggtc gggccaccat taatgttacc
     2461 acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg ccaccgctca taaaaactcc
     2521 ctcttttaag cgttaaaaca attaattaca tgattaatac tgtacataaa accactggtt
     2581 ttatgtacag atcaaaaagt acccgtcatc gttttttctg tcaagttcag gtggaaaatt
     2641 ttttagaaat caggagggat aacacttaga aaacgcagag cgggccacaa aaaattttgt
     2701 ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg atatcacata ctcatgcccc
     2761 ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc gtcccgcttc cactcatgcc
     2821 aggtctgatt aaaaccgagt tgacggaaga gataatccgg catctcttcc ggtttgtgga
     2881 cagcagccaa aagcctgtca tgatggcctt tgagccggaa ctgcatcagg tgcggttccg
     2941 gtgggtacgg agtatcctca tcatggttta tagcttctcc gtaacctgcc aggcataccc
     3001 cgttctccgt gatggtgctg actatcagat gactgtaatc cggcgacgga agcacaagaa
     3061 cggggcaaaa ccctccttcc cggcgaacaa acccgacaag catttcctcc ggccctttgc
     3121 ataacaacag attcagcgtc ctgctgtttc cctctctcga aaatacaaca ggcatgagtt
     3181 gttccggtac agtatcagcg gtgatatcca cccggtgcgc tgacggcagc ggagaggcta
     3241 cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca gtgatgcggc agcgccggaa
     3301 aaccgtaaga gggccacccc tggcgcatct gttccagtac atgccggacc gtcgcaccgg
     3361 aatacattct cacacgctca ggccgcgcca tcgtctcatg agattctggc attccctcgc
     3421 gtatatccga gaccatcaca gccccgcctg gcgacaggta aacattcacc gggtcatgaa
     3481 cactctgggg gtaatgcgcc agctcatcac cccataccgt gaattctccg gctctgttac
     3541 tcaacatcat gtagcgggtt atcctgcccg ttcccggctc tctggcacgc cccagctccg
     3601 taataacccc gactaatgcc gtttcacggc tcagacggtg gttatcatga cgtttcagcc
     3661 gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc ggcttcctcc ctgcgccgct
     3721 ccgcttcccg tgctgacagt ttttctttca gctctgccat tcccggcgca atccacgtat
     3781 tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc ccgttctttc acggccctgt
     3841 aggtgcggcg aagagattca atctgtttcc cgagtttcca gaaacggagt tgtcttttgc
     3901 ggtattccgg ttcagcccat gtcatcaggg gaatctctga cagaagggac gcgacagggc
     3961 gtgctttttt gagtccggtc atctcctttt cgagaatggc ctgctctttc agcagcgcat
     4021 tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc caggaactcc ggggtcatct
     4081 cccacgcgtt cccgcattcc tgcggcagcg tggcaatgaa ttcccgcagg gtatccgctg
     4141 aggcattcgc agcgggaatg gtcaggttaa gggcttccgc tgcacgacgt gcagcatcgc
     4201 tgagttcccg cttacgctgc gcttcctgaa ccgtcctgag agcaagctgc gtgcgggaaa
     4261 cctgtatttc caggttaagg ctgttaatca gacggttctg ctcaccgcgt tctgtttcca
     4321 ttccccgttt ttccatcgca caggcagcct ttccgaggtg aaggcccggc gctttctcca
     4381 gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg gtagccggcg ttcgcaagcg
     4441 ctctgttcgc atggtcagcc catgatgcgc gccacgtctc ggcgagtgcc cggtcgttcc
     4501 agtccctgac ctttcctgcg aatcccgttt ccccgacagc tctcgtggtc agcatgatgt
     4561 gcgcatgggg attggtccgg tccatgtgat ggaacgccac atcggcaatc atgccccgac
     4621 tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg tgcggcctcg cgggaaatct
     4681 cacggggaat ggcaagttca atctcccttg ccagctggga gttccggcgc tgttcggctt
     4741 tctcgaccgc attccagaga acggaacggt caccacacca ggacggtgca ttcgcagggg
     4801 tgagaatgac cgcatcatca acgccgcgtt tacgggtgta atcgtggatt aatccggtac
     4861 ggtcatcggg tatgacagag ccggaacggt aggcagcaga agcaacagca ctgtagccgt
     4921 tttttcgcga aatgattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     4981 tttgccgttt tcttttagcg tcgcagaccg cacacaatcg aaacgaagtg gagattgtat
     5041 aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc agttttgcaa ctaccagtga
     5101 cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa aatgagcaga acactcgaac
     5161 agaagattgc cgaagctgag gccagattac agcgcctgaa ggcgaagagc cggagcctgg
     5221 acacggcgca aaaagtcatt gtgggggctg ccatgctggc gagggtacgg aggccggaag
     5281 aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa ggaagtcaca agacaggccg
     5341 atgtgaaccg gatacaaccg ctgattaacg agctggagaa gctacccaaa ccacctgcga
     5401 aaccgtagaa cggagaaacc ccggcgcgta agtgcccatg caccaccgga cgcctttgcg
     5461 cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagcg ctaccacctc
     5521 aatcatgcgg aaagccacgc cattacgggg cagaacggag gaaatcgcaa gaaagatcac
     5581 tgctcaaaaa gacgccaaaa gggcgctgtt atctgataag gcttatctgg tctcattttg
     5641 cgagatctgg atcacatttt aattttcggt agtacgtcgc tcgactgatc cctaatgcct
     5701 cccacggctt cagcgaacgt gctgatgtag gaactgctgc gcgcttagat ttagttccac
     5761 ccttgcgacc tcgagcggcc tgtacagcgg aaaatccctc tggtgagaat ttcctgtgtg
     5821 tatatttcgc aatgctcttg ccaatagccc gacattcagc cggagaaagc ggaacgggaa
     5881 gcgatgcgtt gtacatttcg acacgctgga tcacggcatc aagccattgt gagaatacag
     5941 gccagccctg acgaatcgct ctgtaggccc atttacggac cttttcgaac agatggtagt
     6001 ttcgccccag cccgtaattt ttatcgacgc tacggcgcgc tgaggcgctc aaatcgagat
     6061 aatcagccag ttcgtcgaga gtgtagggtt cctcgcgcca ttccacttcc tgccattcag
     6121 ggtggcacgg atttttgcag atcaggccgc tgtaattcac atccgcgccc agtttttcac
     6181 acaacgcacg ctcaatagcg gcagcgtatc tgagcgccga agccgatgca tccggcgcag
     6241 ttctcacagg aagggcgagc gcgtagagca gatgcgcgtg cccgttacgg ggatttttta
     6301 cggtgatgtt aggggccgga caattccggt cgctccagtc gattgatgcc cctgtccggt
     6361 ccacgtcaaa gggaagccag tacagtgcat gtggctggtt ctgctggatg aaacgggcaa
     6421 gaatggcaat gtttttcggc agaatgcgaa cgccgtacgc gaaatcgttc gtacagtacg
     6481 gtttgtgcgg taatttttcc ctgaagcgtt gaagtacggc gctcatgtga tacgatttct
     6541 ctcggtacac agtttaacta accgcgcttt cccatcggga aacgcggaac ccggcgccct
     6601 gtaatttcgt gatcaatctt ggcggtgaga gcacgattta cggggcgttt gtttttcagt
     6661 cgcttacact acacccaacg gcaaatcctg tcgatccttc ccgaaaaacc gatccattta
     6721 ccataactcc cgttgtacgt accccttaaa acaccctcaa tcgctttctg acgc
//
LOCUS       DAZOZD010000216         6757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOZD010000216 DAZOZD010000000
VERSION     DAZOZD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.217"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(206..691)
                     /gene="sixA"
                     /locus_tag="ACHUPB_003547"
     CDS             complement(206..691)
                     /gene="sixA"
                     /locus_tag="ACHUPB_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0760270.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(894..3038)
                     /gene="fadJ"
                     /locus_tag="ACHUPB_003548"
     CDS             complement(894..3038)
                     /gene="fadJ"
                     /locus_tag="ACHUPB_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0760271.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3038..4348)
                     /gene="fadI"
                     /locus_tag="ACHUPB_003549"
     CDS             complement(3038..4348)
                     /gene="fadI"
                     /locus_tag="ACHUPB_003549"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0760272.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4528..4812)
                     /locus_tag="ACHUPB_003550"
     CDS             complement(4528..4812)
                     /locus_tag="ACHUPB_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0760273.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5184..6524
                     /gene="fadL"
                     /locus_tag="ACHUPB_003551"
     CDS             5184..6524
                     /gene="fadL"
                     /locus_tag="ACHUPB_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0760274.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1678 a         1815 c         1617 g         1647 t
ORIGIN      
        1 gtgtcaaaca gacattattc atttcaacat gtaactttgg gcaaaatcaa gatacgcgca
       61 gaagaataat ttccgaatga aagtagaaag tgttttattt atagatgggg agagtcttcg
      121 atagtagtga gattgacaga tatccgctgc ctgatgagca attatgccca atgaatctac
      181 ctcattgggc ataatttgga actcatcaga tagcttttgc catcttcaga ttacacgggc
      241 tcatctgcca gttgaatgtg ccgtttccgc tttcatcaag cgttacgctg gcaatggccg
      301 aggtggtaaa cataggcggc gtttcgcccg gacataactc ggcaaccaga tacccgacta
      361 acggtaagtg agagatcacc agtaccgaag caacaccttc attggtcaac gcctgcaaat
      421 aggcactgac cagaccaaca tcgccgcagg gcgttaactc cggcagaact tctgcactgg
      481 aaggcaggtt cagacaatcc cctacttctt ccagtgtttg ctcggctcgc aggaacgggc
      541 tcaccagaac gcgttcgatt tccacttttt gacctttcag ccagttcgcc atcaggcgag
      601 attcgtcaca accattagtg gtcagaggac gaaccgaatc actggcggca tcaagggctg
      661 cgtcgccgtg acgcatgata aaaacttgca tattgcaccg cttttgttaa ccagattcgt
      721 ccgttttctt tcatctgaaa ccagagaaag aaaacggtgg ccggcattgt gccttatcca
      781 ttcaccgaat gaaacgctgt tttttacctc aatggcgtaa gtatagtcaa tatgcgttta
      841 cattttgacc aacactccgc cattcagcgc ggattcatat agctttgacc ttcttattga
      901 aggtcagttg cagttgtttt ccaaaaactt tccccacgcg cgcccatctc gaccaaacgc
      961 tcgcaagggg taaaacggga accatactgc gtggccagtc gttgcattat tgcaaccact
     1021 tcgcccgcgc cgagagaatc gatatagcgg aacggtccac cgagaaatgg cggaaaacca
     1081 atgccaaata ccgcgccaat atccccgtca cgcacgctac ggataacctg ctcatcaaca
     1141 caacgtactg cttcattcag catcaacatc acacaccgtt cagcaacctg cggtgcggag
     1201 agtcgcccct gcccttgtgc gccaatgagc gggtaaatgg cgggatcgac ctgttttttg
     1261 cttttacgcc ctttctgacc ataaagatag aaaccccgac cattttttct gcctttgcga
     1321 tcgtcgttca aaattgaaga aacaacattt gcaggcgcgc taaaacgttc tccataagcg
     1381 gcttccagta caggaataat tttagtcccg gtgtcgattc ctacctcatc caaaagttgg
     1441 attgggccta ccggaaaacc aaatttcact agcgcggcat caatgtgctc tacccgttca
     1501 ccttcggtca gcatgcggat agcttcatta atgtaaggcg ctaagatgcg attgacgtaa
     1561 aacccggctt tgtcacgcac gacaatgggc gttttgccct gttttttcgc cagttttacc
     1621 gtagtggcga tggtttgcgc cgatgtaccc gcatgaggaa taatctctac cagcggcatt
     1681 ttttccacag gactgaagaa atgcaggcca ataacttgct caggtcgcgc ggcgtgagcg
     1741 gcgatatcac caatcggtaa agatgacgtg ttcgaagcaa aaatggtatg agcggcgcaa
     1801 ttttgctcca cttccgccac catctgttgt tttaattcga gattttcaaa caccgcttca
     1861 ataatcagat cgcgatgggc aaagccgcga tagtccgtcg ttccggagat taatgccagc
     1921 tgtttgtcac gttcgctggc tttgagatga cgacggcgaa ctttgccctc cagctgatcc
     1981 caactgtact tcagcgcatg atttatgccc tgcgggttga tatctttaat tctgaccgga
     2041 agccccgctt tacaagcagt gacataagca ataccgccgc ccatcaagcc accacctaaa
     2101 attcccacgc tgtttaatgg cgcaggcggt gcatcactac cgggatcttt cttcacgtcc
     2161 gtactggcaa aaaagatact acgcaaagct tgcgattgcg gcgtcatcgc cagttcgcca
     2221 aaggcgcgag cttcagcgtc atagccgctg ctggtgccct gcgctaatcc cgtttcaaca
     2281 acctccagga tgcgttctgt cgccggataa ttgccttgag ttttgtgttc tgttttcttg
     2341 ccgaccattt tgaacagcag cgcacgacct aacggccccg ccagaatacg ctcgcgtaca
     2401 ggtagagggc gggaagatgg gcgatccttc ttcgccagct caacagcggc ttccagcaaa
     2461 atggagtgcg gaacaacgtc atccaccagc cccagcttta atgcctgttt cgcccgaagt
     2521 tgttttccgg tgagaatcat ctctaatgct gtactgacgc ctatcagacg cggtaaacgc
     2581 tgggtgccgc ctgaaccggg taacaatcca agttgtactt caggcaaacc gagcaccgtt
     2641 ttaggatcgt cagtacaaac gcgaccgtgg cacgccagcg ccagctccag cccaccgccc
     2701 aggcaagcgc catgaatagc cgcgataacc ggaatgggca aagcatgaat ctccgccatc
     2761 aactgttggc cctgccgcgc cagtgcttcc gcttcttgcg ccgttttgca gttgccaatc
     2821 atgttgatgt ctgctccggc gataaaattg tccggtttcg cggagataaa caccacgccg
     2881 cgcaactctt tgttttcacg gagttgctta ataatggcgc gcacctgcga ggcaaattcc
     2941 gccttcaggg tattcatttt ctcatccggt acatcgatgg tgatgatagc aatgttgtcc
     3001 agacgaacat taagagtaaa cgctgatgcc atttccatta ttccgcctcc agaaccattg
     3061 ccgcgccaag cccaccggca gcacaggcgg taactaaacc aaatccaccg ccgcgacggc
     3121 gaagttcatg caatgtctgg gtaatcatcc gcgcgccggt cgccgcgaag ggatgcccgt
     3181 aagcaatcga accgccaagc acgttaaatt tgctatcgtc cacttcgcca gtggcatgtg
     3241 cacgccccag tacttcacga gcaaaacgtt cactacccag caactgaata ttcgccagtg
     3301 tctgagctgc aaaggcttcg tgcatatcga tcaatgtcag ttcgctcatc gtcaaaccgg
     3361 cacgttccag cgccagcggt gttgaccagg ctggaccgag caacatgtcc tgccagacat
     3421 caatcgcagt aaatgcgtag ctgcgcagat accccagcgg caccagccct aattctttcg
     3481 cccgggattc agtcatcagg atcaccgctg ccgcgccatc ggtcagcggc gtactgtttg
     3541 ccgccgttac cgttccgtgt ttgcgatcaa acgccgggcg cagctttgcg taatcggcaa
     3601 gcgaggaatt accgcgaata ttgttgtctt cgacaagcgg ttgtttataa ggagggataa
     3661 aggcagtcat cacctcttct ttgagttttc cgtctgacca tgcctgagcg gcacgctgat
     3721 gcgaacggtg cgctaatgca tcttgctgtt ctcgggtgat gccgtaggtt ttcgccattt
     3781 gttcggcggt gtcgcccatc cgcaagccag tagaatattc tgctaccgca ggcggtacgg
     3841 gcattaagtc gcacaaacgc aggcgagaga agagtttcag gcgctggctc atggttcgag
     3901 ctttgttgac atcaaccagc acgcgcgcca gttttttact gacgccaatt ggcaatacgg
     3961 aagaggaatc tgccccaccg gcaatccccg ctcggatagt tcccgccatc aggctttctg
     4021 cgacgtttgc aaccgcctgg aaactggtag cgcaagcgcg gctgacgctg taagcatcgg
     4081 tatgcacatt cattccggta ccgagaacaa tttcacgcgc aatgttgggg gcttcaggca
     4141 tttgtacgac ctgaccaaag accagctgtt caatcacttc agcggggatc tcgctgcgtg
     4201 ccagcagttc gcctaccacc atcttcccta aatcaaccgc gggaatgcca tgaaaagccg
     4261 tcgcctggcg ggcaaaaggc gtacgtaaac cgctaacaat ggcgatacga tcgccctggc
     4321 gggtaaccag cggtaaaacc tgacccataa cactcccctg taaaaaaata aataaagtgg
     4381 tctgacctga tcatagtctt aactattttt ttacatttag ccaagcggag aaaaaggaaa
     4441 gtgggagcta tgacacagtg aagacaggag aaagaaaaga aaacgcccct gccagcaact
     4501 gacaggggcg aacgtacccg agaggaatta acgcagaccc agctggaaga tcagcatttc
     4561 agcttcgcag gcaaaagtga aatcgatatc caggcgcaca ccgtcagact cttcagtgaa
     4621 agtcggggag attttgcacg gttcggattc cacgctacgg gctttttcag tcagcgccgc
     4681 cagcgtttgt tctgcttctg cgcggtttgc aaacacgcgg ctgtaagacg cggtgcagtc
     4741 ggagttgtcc ataatggtgc caacatccat acagcagcaa accggggttt catcagcact
     4801 acatttactc atcgttgatt tcctctgtat gtgcacccaa ggtgccagat aaacgttgtg
     4861 gatattttac gcttccggaa agtgctgctc cagttgttaa ttctgcaaaa tcggataagt
     4921 gaccgaaatc acacttaaaa atgatctaaa acaaaattca cccgaatccg cgagtgcgca
     4981 acctccaaat tttgccagct ggatcgcgtt tcttagatca tatttgaaaa aagatggaaa
     5041 catacttgca gcattccagc tggtccgacc tatactctcg ccactggtct gatttctaag
     5101 atgtacctca gaccctacac ttcgcgctcc tgttacagca cgtaacatag tttgtataaa
     5161 aataaatcat tgaggttatg gtcatgagcc agaaaaccct gtttacaaag tctgctctcg
     5221 cagtcgcagt ggcacttatc tccacccagg cctggtcggc aggctttcag ttaaacgaat
     5281 tttcttcctc tggcctgggc cgggcttatt caggggaagg cgcaattgcc gatgatgcag
     5341 gtaacgttag ccgtaacccc gcattgatta ccatgtttga ccgcccgaca ttttctgcgg
     5401 gtgcggttta tattgacccg gatgtgaata tcagcggaac gtctccgtct ggtcgtagcc
     5461 tgaaagccga taacatcgcg cctacggcat gggttccgaa catgcacttt gttgcaccga
     5521 ttaacgacca atttggttgg ggcgcttcta ttacctctaa ctatggtctg gcaacagagt
     5581 ttaatgatac ttatgcaggc ggctctgtcg ggggtacaac tgaccttgaa accatgaacc
     5641 tgaacttaag cggtgcgtat cgcttaaata atgcatggag ctttggtctt ggtttcaacg
     5701 ccgtctacgc tcgcgcgaaa attgaacgtt tcgcaggcga tctggggcag ttggttgctg
     5761 gtcagattat gcaatctcct gccgggcaaa ctcctcaagg gcaagcattg gcagctaccg
     5821 ccaacggtat cgacagtaat accaaaatcg ctcatctgaa cggtaaccag tggggctttg
     5881 gctggaacgc cggaatcctg tatgaactgg ataaaaataa ccgctatgca ctgacctacc
     5941 gttctgaagt gaaaattgac ttcaaaggta actacagcag cgatcttaat cgtgcgttta
     6001 ataactacgg tttgccaatt cctaccgcga caggtggcgc aacgcaatcg ggttatctga
     6061 cgctgaacct gcctgaaatg tgggaagtgt caggttataa ccgtgttgat ccacagtggg
     6121 cgattcacta tagcctggct tacaccagct ggagtcagtt ccagcagctg aaagcgacct
     6181 caaccagtgg cgacacgctg ttccagaaac atgaaggctt taaagatgct taccgcatcg
     6241 cgttgggtac cacttattac tacgatgata actggacctt ccgtaccggt atcgcctttg
     6301 atgacagccc agttccggct cagaatcgtt ctatctccat tccggaccag gaccgtttct
     6361 ggctgagtgc aggtacgact tacgcattta ataaagatgc ttcagtcgac gttggtgttt
     6421 cttatatgca cggtcagagc gtgaaaatta acgaaggccc ataccagttc gagtctgaag
     6481 gtaaagcctg gctgttcggt actaacttta actacgcgtt ctgataacgc gttcgcctgg
     6541 ataaagtcac ctgcatagca ggtgacttta actcccccac ttcaccgaaa gtagtgtcct
     6601 cattgcttac ctcctgagtt ttgcaaacag cctgttggca gcttgccctt tcaaatcaat
     6661 aagcggtatc cacagacaaa cctgaaggga aaggcatatt ttcaggcgtt ctgctcgtcc
     6721 ttctcaaaga gtttactttt ctgcatttcc aggatac
//
LOCUS       DAZOZD010000217         6682 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOZD010000217 DAZOZD010000000
VERSION     DAZOZD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.219"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(442..3984)
                     /locus_tag="ACHUPB_003552"
                     /pseudo
     CDS             complement(442..3984)
                     /locus_tag="ACHUPB_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4052..6160)
                     /locus_tag="ACHUPB_003553"
     CDS             complement(4052..6160)
                     /locus_tag="ACHUPB_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR0760275.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT         1297 a         2081 c         1725 g         1579 t
ORIGIN      
        1 cgtcccaagc tcttcaaaaa acttcattgg agaatctttg atattctcaa tcttttcacc
       61 ataaacgtct aatattgatg gttcaccagg gtcatcaaac tgctcaccat aaggtaaaat
      121 acccaataaa tttctccaac acctatctct accattttcc tgaagtgatt ctgataattg
      181 taaatttgct ctgttatatg cagctgtcgc agattttata ttccacgccc ctaaagccat
      241 agaagctgca ccgactgggg cagcaatgat cgttccatcg gcagcaacac ttataccgaa
      301 ttttgtcatg ccacttgcat acagccttgc tgaattagca gtatttaaac tagctgtaaa
      361 catttttgtc gggttaaacg gaaggttatc cagtttatta tttatatcat ctataaaatg
      421 actacaactc tcacatgaga ataaccccaa cggatctatc ctgctcaccg gattcagcgg
      481 atactgatac agattccacc cacctttcag cccaatgggg tcctgggtga tgtaccgtct
      541 ctgcaacgga tcatagtacc ggtgacgatt gtagtacagc cccgactcct catcatactg
      601 ctgccccggc agacggtatg gctgatacac atgatgcggg ttctcctcat ttagctggtt
      661 gccccactca tcatacgccc cgctccacac cgtatcgccg tcttcgctga tgagcgccag
      721 cggctggccc cgatggtcgc agtggtaaag atgaacgttt cgcactggtg tgtattccgg
      781 ctccacctgt ctggccagtt gctccaccgt cagcccgcac tgcgcaagcc acacccggct
      841 ttcactgctc acgcggtctg cccggatttc ttcctccagt ctgtccagca gccgcaccag
      901 ttcagccgga aataccacgc cgtgcccgtc ctcgctccct tcctgctgca gcttctccgc
      961 caggctgcgg cgctgcgctt tctcccgctc gccgttctct gtctcgaccc ggatgagcgg
     1021 cgtgaagctt cccggctcgt ataccgtctg gatacgtgtg gtgtcggtct gtaccgtcgt
     1081 cagcctgtcg ccgtcccagc cgtaccatgt ctcctccggt ttacgcgaca gcgacatcca
     1141 ccccgtcagg tcacgctccc gccgccatac ccgtttcgcc attcgccgtc ccagcgggtc
     1201 gtagaggtag cggctctcga ccagtggctc gccatgctgt atccgcgtgt ggaacaccag
     1261 gcggtgctgg ctgtcgtagt ggtgggtccg ctcgtcgtcc gtccgtatca cacccgtcgg
     1321 gatgcggtcc gtcttctccg tcagtctgcc gtattcatca tagtggtaaa catagtgcgc
     1381 atcctctgcg atgcggttat ccggccacgc cgtgagcgtg ctgtccgggt gtagctccgg
     1441 gtccggcagg cggtttcccg ccgggtcggt ggcatacggg atgcggatgt ccaggtccgg
     1501 tgcgagggtg cgcaccgtct cacggtgcag cctgtcccgc gtatactcga ccagcggcgt
     1561 cccgcccagc ttcatgcccg ccaggtaacc gctgccgtag gtcagccact ccaccggcgg
     1621 caggctgtcc ggcgtgacgc ggtttgccag cccctgttcg ttgtaggcgt gtttcgtctc
     1681 atgctgccac agcagttccc ccgtctccgg gttctccacc gtctgccgtt cgcccgtcag
     1741 gcggccctta tcgtcgtagc cgtagtggac agcgacacgg tggccttcgc tcaggtggct
     1801 gatttctctc agccagccgt ggccgtcata ctgccactgc tctgccgggt cgccgttcac
     1861 cgtccggtgc gtgatgcggt ccgacgcatc gtagtgccag agggtgacaa gcccctcatc
     1921 ctcactctgc gtgagttttc cggtcaggtc ataatgataa cgttgcgtcc gcccgtcaaa
     1981 gccgccctgc tgtaccagcc ggtccagcgc atcgtagctg aagtcgctgt gactgccgtt
     2041 ctcgttagtc agactgatga cacgtccggc agcatcgtac tccatactgc gcgtcagccc
     2101 gccctgcgtg gtgctgaccg cctttcccca cgcatcgtac tgtgtctcgc tccggttgcc
     2161 gtccggggtg ataacggcag tcaggtcgcc tgcggcgttg tactcatact gcgtttcacg
     2221 gccctgtgcg tctttcaccg aggttaaccg gccacggttg tcatagtggc ggtaaaggct
     2281 gataccttcc tcgcggtgaa ccgccgtcat ctggccgaag cggtcgtatt catagcgggt
     2341 ctggtagccc gagcagtcag tgaacgccag caactgcccg tagcggctcc acgtcatctg
     2401 ccgggtgctg cccgtcgcat ccgttgtcgt cgccggtaac tcactgtgcg cgtcatcgta
     2461 gcggtagcgt actgtctccc cgctgcgcga tgtctccgat accagcctgc ccggttcatc
     2521 atattcccgg cggctctcca gcccgtccgg gtacaccacc gccgtcagct ggttcccgtc
     2581 gttatagtaa aatttcgtct cccgcccgtc cggtgtggtg atgtccgtga tatcgccgga
     2641 caccacattc agaccgtact ctgtccgccg tcccgccgcg tccgtctgcg ccgtgagcct
     2701 tcctgccgcg tcatacccgc tgcgcgtgac gctgccgtcc gccagttctt ttttcaccac
     2761 ccgtttcagc ccggccccgc cttctgtatg cagcacctca cgccggttca ggctgtcggt
     2821 gacggtgatg cggtcctgct cataaagata gcggtagctt aaccctgccg ggttcagttg
     2881 ctccaccacc cgccccgtat cgtcgtagcg gtagcgcatc tccggccttc ccgcgtaacg
     2941 gtgcgccacc atccggcccg ggcgctgcgc gtcatacgtg aaagcgcgca cctgcgtatt
     3001 gctgcggtca tataccgcca gcagttcacc ggcttccgta tacgtgtacc gcaccagtgg
     3061 cgcagcgggc aggctctccg ggtatgccgg gtcgtgcatc aaccacaccg ccgaaaggcg
     3121 gatacccctg tcggggccgt attcggtacc gggcagtgtg tcggggaacg ctgaggctga
     3181 gagagggcgg gaactgtcag aagaagatag cgaagaggtg cgggcctctt ccgcacgctg
     3241 cgcctgcgtg gtcagcacca gacggaactc ccgcccggca ccgtccgtca cgtcggtgat
     3301 ttccccggcc aggtcaccgg cggcctcacg ccggtacgtc agcgtccgcc atcccggtaa
     3361 gcacccggta cggcggcagc ggcgctggca gtacgtcctc agcacccggc acccgctcag
     3421 ccccccccag tatccaccac ggcccctgtg cgctgttggt cgccaggtaa agatgcgggc
     3481 ttaaccggat atccggcgca gcgcccccca cagccgcgcc agcgtatggc cgtccggctg
     3541 tgctgcctta ccaccgcgca ccagccacat tgactcactg cggctgtaca ccgcctcccc
     3601 cggcagcagc ggctcaaagt gaatgctccg cccgccgttg tcgttgagta tcagtccgtc
     3661 atcacgtagc tgtaagcgga tatcagaagg cgctttccag ccggggccga aaacgcccac
     3721 cggtgcaggc gtcttcgtcc ggtagctgct gtaggtgcgg gagagaatga acggcagcgg
     3781 gccgggcagc gcaaggtccg tctcgccggg cagcaccttc gcccccagca gcggatttac
     3841 cgggttgccc gaagtcatcc cgcccggaca caccgagcac gccacgccgg tgggcgcgcc
     3901 aattcttaca cctgccgaac cctggacaat gggaccgcca tactgagtca tatctccctg
     3961 acgcgccgct ggttttccgc tcatgattca aattccttct tctgtttatt tttaatgctg
     4021 gtttgttact gaataccgaa caggtacggg atcagtactc tctcatatcc ggcgtatcct
     4081 gcttttcatc aggggtcttc ggtggggatt tcgaggctgc acagttccag ttaatcaaca
     4141 gtgagtcacc attaacctgc tccttaccct gcaaatgaat ttttgtgccg ttgagaaaaa
     4201 tttgtccgtc cttcgtcagc accagccttg ccttaccaca gcagagctcc agatgctcac
     4261 cggcggagta aatcgcggtc tgcccggtat catccttttt cgttttacca acggtgaagg
     4321 taacgttatt tccgactttg acgtcataac ccagcccgac cctcaacgat ttatgcaaac
     4381 cgatctgcga ggactgcatc agtgccaccg aggtgttcat aatgccacct accgtcgtct
     4441 gatacgccac gcccaccgtc agcgcacgaa caagtccaat cgtttccacc tgaacagaac
     4501 ccactttaat tatctggtta ctgcccaccg tctctatctg gttaacgccc accgtctcta
     4561 tctgattgtt ggtaaccgca atcatctgat tgttgccaat ggtttcagca tggttgttta
     4621 tcacatccgt ggtgcggtta ttcagcacct cggtgttcat gttcttctgc gcgtggatgt
     4681 agacctgttc tttccctgtc gcatcgtcaa acttcagttc attaaacccg ctgcccttat
     4741 aggttttcga acgaatggtc atctgcgtct tcgtccccgg caggctgccg ggtgtgcggt
     4801 tttcctggtg gtaggtacgc cccataataa tcggctggtc cgggtcgccg ttgaggaagt
     4861 ccacaatcac ctcctgaccc acacgcggta tcgccaggtt gccaaagccg gtgcctgccc
     4921 acgcctgtgc cacacggatc cagcatgaac tgtcctggtt tgacgggtta tagcggtccc
     4981 agttaaattt cacccgcacg cggccatgtt catcacagaa gatttcctcg cctgccggtc
     5041 ccgtcacgac ggcgctctgc gggccatcca ccagcggttt catcaacggc tgtggccgcc
     5101 atgttctgtc tgccgggatt accgcaaaat ggttctccag cgtggtgcct gaacccctgc
     5161 gtcccggtac cgcctgcggc tgttcgccgt gaagatcgct cgccaccacc tgccattccc
     5221 ggttcaggtt cgcctgcgga tgccccgtca gcacaattcg ccgtcccggc catatctccg
     5281 gcgaacggct tgttccgcgc gccacttctg cgttgtttcg ccagccgtcc atctgccagc
     5341 gggcaaagtt ctgcccgtgg gcacccttga aacgtccggg gtagtcatac acttcatact
     5401 gcgtgcgctg gtaatcctgg tactggcctt cctgatcaaa acgccctggc cagccgggac
     5461 gtttaaaggt gtagtctttg gtcactacgg aagaaggacg gatttgtgcg ctgtagcgaa
     5521 actggctgat gcagagggta cttacctcgg tacgggtgtt cgggttccag gggatctcaa
     5581 aggactccgg cagataacgc acggtgtcgc acaggaccag gctctggtcg gtactttttt
     5641 gcgcgtgctc ctcataaaag aagatgcctt cctccgccgc catccggcac aggaaatcgt
     5701 aatcagtttc gccgtactgg acacaaaact cacgggaagg atgcggctcg ctgaacagcg
     5761 gactccactc ggtcacaccg ttttccttca ggatcgtcgc gaggatgctt tcgatgtcct
     5821 cattctggaa gatacggaag ttctggcgca gccctatgcg ccacagcggt gggcatacct
     5881 tcatgctgta cagcatctgg tttttgtcgt tctcccccag ttcaaaccag gtcaccacac
     5941 ctttcacccg gcgctgtacg tcatcgccct gccataccgt caggtaggcc attttgtcca
     6001 gcacctgcgc aaattcaagg gagagaaact gctggctgac cagagagaga tcgagggaaa
     6061 agagcgaaga gagtgactgg ttcaaatgaa aggaaaccac cgcaaaagca tccggtggca
     6121 ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc gtgaaaatga
     6181 ataataatcc gcacatattg catgtacaac atgtcgggcg gatgagataa gcgagtcgac
     6241 atctggcgtt accggggaat atcagaaaaa tgtcatggtt atgtgatgat ttagtctggg
     6301 attttacttc agagaaacgg gtgtctgttt agtcaacaaa ataatcacaa acgcaacgga
     6361 tgggttctcg acgttattca cgacaggctc gtacattaca gcgagtaata aaaaacctga
     6421 tatgcaaagt caaaccaata ttcataatgt ctttttattc acaacaataa ataattcaat
     6481 aacaatcatt gaaatgaatg atggtttgta taaggaatat acgctaccgc ccaccctgat
     6541 aacatgatct gcacattgca catcttaacg ttgttaaata ctggctcaat agtggatatt
     6601 ttaaattagg aatttatcaa attcacttga caaatagatt gagtgaataa tataacaatt
     6661 aatgatgatt aatcgcgatt aa
//
LOCUS       DAZOZD010000218         6646 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOZD010000218 DAZOZD010000000
VERSION     DAZOZD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.220"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            55..717
                     /locus_tag="ACHUPB_003554"
     CDS             55..717
                     /locus_tag="ACHUPB_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0760276.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            813..1271
                     /gene="mgsA"
                     /locus_tag="ACHUPB_003555"
     CDS             813..1271
                     /gene="mgsA"
                     /locus_tag="ACHUPB_003555"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0760277.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(1303..3357)
                     /gene="helD"
                     /locus_tag="ACHUPB_003556"
     CDS             complement(1303..3357)
                     /gene="helD"
                     /locus_tag="ACHUPB_003556"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0760278.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            3480..3926
                     /locus_tag="ACHUPB_003557"
     CDS             3480..3926
                     /locus_tag="ACHUPB_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0760279.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            3936..6098
                     /gene="yccS"
                     /locus_tag="ACHUPB_003558"
     CDS             3936..6098
                     /gene="yccS"
                     /locus_tag="ACHUPB_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0760280.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(6061..6645)
                     /gene="sxy"
                     /locus_tag="ACHUPB_003559"
     CDS             complement(6061..6645)
                     /gene="sxy"
                     /locus_tag="ACHUPB_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0760281.1"
                     /translation="MATLGTIEYRSLFGSYSLTVDDTVFAMVSDGELYLRACEQSAQY
                     CVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYSLDAALKEKSTRNTRE
                     RLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQNSLVTEKILFMLEGAI
                     IGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
BASE COUNT         1517 a         1787 c         1616 g         1726 t
ORIGIN      
        1 aaagaaaaat tttgacacat tattattcgg gcggtctttt gacaggagct acctatgaaa
       61 accggcatcg tgaccacctt aattgcatta tgtttgccgg tatccgtttt tgccaccacg
      121 ctgcggcttt caaccgatgt cgatcttctg gtactcgacg gcaagaaggt ttccagctct
      181 ctgctgcgcg gtgcggacag cattgaactg gataatggac cgcatcagtt agtgtttcgc
      241 gttgagaaga cgattcatct ttccaatagt gaagaacggc tgtatatctc cccaccgctg
      301 gtggtcagtt ttaataccca gctcatcaac caggtcaatt ttcgcctgcc tcgcctggag
      361 aatgagcggg aagctaacca ttttgatgcc gcgccgcgcc ttgaattgtt ggatggcgat
      421 gcgacgccga ttccggtaaa gctggatatt ctcgccatta cctcaacggc aaaaacgatt
      481 gattatgagg tcgaggttga acgctataac aaatccgcga aacgcgcttc actaccgcaa
      541 tttgccacga tgatggcaga tgacagtacg ctgctttcgg gtgtttccga gctggatgct
      601 attccaccgc agtctcaggt gctcacagaa caacggctga agtattggtt taaactggct
      661 gacccacaaa cgcgaaatac tttcctgcag tgggcggaaa aacaaccatc ttcctgagat
      721 ttttgtgcct gtgcgcaggc tttttcggtc tttatcttgc agcgataagt gcttacagta
      781 atctgtagga aagttaacta cggatgtaca ttatggaact gacgactcgc actttacctg
      841 cgcggaaaca tattgcgctg gtggcacacg atcactgcaa acaaatgctg atgagctggg
      901 tggaacggca tcaaccgtta ctggaacaac acgtactgta tgcaacaggc actaccggta
      961 acttaatttc ccgcgcgacc ggcatgaacg tcaacgcgat gttgagtggc ccaatggggg
     1021 gtgaccagca ggttggcgca ttgatctcag aagggaaaat tgatgtattg attttcttct
     1081 gggatccact aaatgccgtg ccgcacgatc ctgacgtgaa agccttgctg cgtctggcga
     1141 cggtatggaa cattccggtc gccaccaacg tggcaacggc agacttcata atccagtcgc
     1201 cgcatttcaa cgacgcggtc gatattctaa tccccgatta tcagcgttat ctcgcggacc
     1261 gtctgaagta atattgcaca ggtggcaaac gccacctgtt tcttacggtt ttctcgccac
     1321 cggcacatcc agatttttca gtatttccac aaagggagag ggattctctt tgttaaacag
     1381 tgcccatacc cgatggcgtg cccgggtcag cgccacgtac attaaccgcc gttcttcagc
     1441 gtccgggaaa tcctcaaccg gtggcagtag cgcctcttcc ataatcgact cccgcgccgc
     1501 agccggaaaa ccatcacttc cctcctgcaa gccaacgatg atgacgtaat ccgcctgttg
     1561 ccctttgctg gcatgaatgg tcataaagtc gatttgcaac ttcggccagc gtgttgccgc
     1621 tttttccagg ctggcaggcc tcatgtgatg gtaacgcgcc aggatcagaa tgcgctcttc
     1681 cggtttggca taaccagaga gcttatccag caaagcgtca agttgactct catccaataa
     1741 cgtgacggct tttttgtctc cattggttaa gctgtttagc ggctttttca gctggcctgg
     1801 gttctgctga ataaaccggt ttgccacctt accgatacga ctgttaaaac ggtaagtcgt
     1861 gtctaaatca caacgttcgc cttcaccaaa gttttcatgg aaagcggtgg tgagcgacat
     1921 ttgcgcaccg ctgaatcggt aaatcgcctg ccagtcatca ccaacagcga acaacgtcgt
     1981 ctgactgttt tgcttgcgta atgccgctaa caacgctgcc cgctgcgacg agatatcctg
     2041 aaattcatca accagaatat gcttccacgg gctgataaag cgacctttct ccagaatcac
     2101 aatcgcctga tgaataaggc ccgaaaaatc gacagcgttt tctgccttca gcgcaccttt
     2161 ccaggctttt aataacgggg ccatcaactt gatacgttta ctgaacagat cgcgaatctc
     2221 ttcgggtgca ctggcaatca tttctgcctg tgcaccaccg tgcatccgca tcagacttac
     2281 ccagcgatcg aggcgagagg caaggcgacg ctgtaatttt tcatcatccc agaagttacc
     2341 ttctggcact gaccactgca tttcttccgt cagccattgc cgccagccct tcgcctgcgc
     2401 tttcttttcg ctgcattgct tgcgccactc agcaataaag agttcatgac gggcagcggt
     2461 atcattttcc agtttgctga ctatcggaac ttttttgctg ccctgctgaa taatatgcag
     2521 cgccagcgca tgaaacgtgc gtgcggtaat gtcttcggta tgtagccgtt cgcgaatccg
     2581 ctcgtccatc tcttcagcgg ctttgcgacc aaacgccagc aataaaattt gctcagggga
     2641 cgcttcacca cgcgccagca accagcctgc acgggccacc agcaccgacg tttttccgct
     2701 tcctgcacct gccagcacta acagagaatg ctcgccatta acgactgccc gggcctgcgc
     2761 cggattcagc ggtgaagact cgacctggcg gaaaaaatcc gcatactcgg taagcatggc
     2821 ttcggtatac gcctggttat gctgcaaccg agcgctttca atatctttca accaggcctg
     2881 acatttacgc cacgcctcac ggcagttatc gaattcttcc agtcggttaa ccggcaacgg
     2941 caacgccgac aaagcctggc ggatttgttg ctgaacgcca gaggtttgct cacgcgtcag
     3001 ccatttattt tctccagtgc gcgtggcaat caaatccagt tgctggcgta aaacaccaga
     3061 cgcaatttcg ctcatctcgc cactccaccg ccgccagtga gcatcaagat gatggtaaaa
     3121 acgctgcgtc tcgccccatt cggtgccgtg cagacgcacc actttttcgt ccggcagtac
     3181 aaattccagc tcgccccata ccagaccgcg cttacagtga atcgccagta attgattgaa
     3241 aggaataagg tattcgtggc gatcgccgga gactttaatc ccggcattga ggatcaccgc
     3301 ccgatcgtaa gggtgctgtg ccagacgttt tccaagcgtt gtcgctttca gttccatgac
     3361 tctgtagatc cacacgaaat tatttcttat cagtgtaacc gtcagagaaa acgtgctcca
     3421 gcccaaaaaa acgttacaat tgccgcccat tactgaaaac cacagtaaag cgaggtttta
     3481 tgcgtaccgt tttgaacatt ctgaactttg tgcttggcgg atttgccacc actctgggct
     3541 ggctgttggc gactctggtc agtattgtgc tgatttttac cttaccgctg acacgatcct
     3601 gctgggagat cactaaacta tctctggtgc cttatggcaa tgaagctatt catgtcgatg
     3661 aactgaaccc ggctggcaaa aatgtgctgc tgaatactgg cggtacggta ttgaatattt
     3721 tctggctgat tttctttggc tggtggttat gcctgatgca cattgcaacg ggcatcgcac
     3781 aatgtatttc aatcattggc attcctgtcg gcattgcgaa ctttaaaatt gccgctattg
     3841 cactatggcc ggttggtcgt cgcgtggtat cggtagaaac agcgcaagct gcgcgtgaag
     3901 ccaatgcacg tcgtcgtttt gaataatcca ctaatatggc ctttatgcta agtcctttgc
     3961 tcaaacgcta tacctggaac agcgcctggc tgtattacgc gcgtattttt attgcgcttt
     4021 gtggaaccac agcgtttccg tggtggctgg gtgatgtaaa actgacgatt ccgctaacgc
     4081 tggggatggt ggcagcggcg ctgaccgatc tcgatgaccg actggcggga cgtttgcgta
     4141 acctcatcat tacgctgttc tgctttttta tcgcctcggc ctcagtagaa ttgctgtttc
     4201 cctggccctg gctatttgcg attggcttaa cgctctctac cagcggcttc attttgctcg
     4261 gcggtctggg tcaacgctat gcaacaattg ccttcggtgc attgctgatc gccatttaca
     4321 ctatgttggg aacatcactg tatgagcact ggtatcagca gccgatgtat ctgctggccg
     4381 gtgccgtctg gtacaacgtc ctgacactta ttggtcatct gctgttcccg gtccgcccgc
     4441 tgcaggacaa cctggcgcgt tgctatgaac aactggcgcg ttatcttgag ctcaagtcgc
     4501 gcatgtttga tcctgatatt gaagatgaaa gccaggcacc gctgtacgat ttggctctcg
     4561 ccaacggtct gctgatggcg acattgaatc agacgaaact ctcgctgctg acccgcttac
     4621 gtggcgatcg tggtcaacgg ggaacgcgtc gcacgctgca ttattacttt gtcgcacagg
     4681 atattcacga gcgtgccagc tcttctcata ttcagtatca aacattgcgt gaacattttc
     4741 gccacagcga cgtgctgttc cgttttcagc ggctgatgtc gatgcagggc caggcgtgcc
     4801 agcaactgtc acgctgtatt ttgttgcgtc agccttatca acatgatccg cattttgagc
     4861 gcgcttttac gcatattgat gctgcgctgg agcggatgcg cgataacggc gcacccgccg
     4921 atttactcaa aacactggga tttttgctga acaatttacg cgccattgat gcccaactgg
     4981 caacaattga atcagaacag gcccaggcac taccccataa taatgacgaa aatgagctcg
     5041 ctgatgacag cccgcacggg ttgagtgata tctggctgcg tcttagccgt cacttcacgc
     5101 cggaatccgc cctcttccgt catgcggtaa gaatgtcgct ggtattgtgc ttcggctacg
     5161 ccatcattca gataaccgga atgcatcacg ggtattggat cttgctgaca agtttgtttg
     5221 tctgccagcc aaactataac gccacgcgcc accgcctgaa gttaaggatt attggtacgc
     5281 tggtaggtat cgccattggc attcctgtgc tgtggtttgt gccatcactg gaagggcagc
     5341 tggtgctgct ggttattacc ggcgtgctct tttttgcctt ccgtaacgtg caatacgctc
     5401 atgcaacgat gttcatcaca cttttggtgc tactgtgttt taacttactg ggtgaaggtt
     5461 ttgaagtagc gttacctcgc gtaatcgata cgctgattgg ttgtgccatt gcgtgggcgg
     5521 cagtgagcta catctggcct gactggcagt ttcgcaatct gccgcgcatg ctcgaacgcg
     5581 ccacagaggc caactgtcgg tatctcgatg ccatactgga gcaataccat caggggcgtg
     5641 ataaccgtct ggcgtatcgt attgcccgcc gcgatgcaca caaccgtgat gctgagctgg
     5701 cgtcggtggt atcaaatatg tccagcgagc cgaacgttac cccgcaaatt cgcgaagccg
     5761 cgtttcggtt gctgtgcctt aaccatacgt ttaccagcta tatctcagcc ctcggtgctc
     5821 accgggagca gttaactaat cctgaaattc tggcgtttct tgatgacgca gtttgctatg
     5881 ttgatgacgc gttacatcat caacctgctg atgaagaacg cgtcaatgag gcattagcta
     5941 gcctgaaaca gcggatgcag caacttgaac cacgggcaga cagcaaagaa cctctggtcg
     6001 tacaacaagt tggattattg attgcattac tgcctgagat tggtcgtctg caacgccaga
     6061 ttactcaagt tccgcaggaa actcctgttt cggcgtaaga gagtcagccc attctgcaag
     6121 ctcctggcgg cgtgccaccg ggagcgcagc ttcatgaatg ccgataatgg caccttcaag
     6181 cataaacaga atcttttctg tcaccagact gttttgctgc cgcagtcgca accaacacat
     6241 ttttgcccca agtatacgta acgcccgtac atccttaatc cccacctccc cgagaatcgc
     6301 ttccagatga aaagacatat tgggcaaatc tttcagtctt tcccgggtat tgcgcgtgct
     6361 tttctctttc agcgctgcat cgagagaata tttcgacaga cgcaccagct tcagttgatt
     6421 tcgccataga ctttcatcaa cccgatagta attgagggta acggatcggc cacacttttt
     6481 atatgtcagc cagacaggcg gatgttttac acagtactgt gcactttgct cacaagcccg
     6541 aagatacaac tcaccatcag aaaccatcgc aaacaccgtg tcgtcaacgg tcaggctgta
     6601 actgccaaac aatgatcggt attcaattgt gcccaacgtt gccagg
//
LOCUS       DAZOZD010000219         6594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZOZD010000219 DAZOZD010000000
VERSION     DAZOZD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.221"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            52..936
                     /locus_tag="ACHUPB_003560"
     CDS             52..936
                     /locus_tag="ACHUPB_003560"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0760282.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHUPB_003561"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHUPB_003561"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR0760283.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHUPB_003562"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHUPB_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0760284.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHUPB_003563"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHUPB_003563"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0760285.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="ACHUPB_003564"
     CDS             5160..6443
                     /locus_tag="ACHUPB_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR0760286.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         1728 a         1632 c         1605 g         1629 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgat
//
LOCUS       DAZOZD010000220         6537 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZOZD010000220 DAZOZD010000000
VERSION     DAZOZD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.222"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUPB_003565"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUPB_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0760287.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUPB_003566"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUPB_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0760288.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHUPB_003567"
     CDS             2408..3157
                     /locus_tag="ACHUPB_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0760289.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUPB_003568"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="ACHUPB_003568"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUPB_003569"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHUPB_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0760290.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUPB_003570"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="ACHUPB_003570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0760291.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="ACHUPB_003571"
     CDS             6018..6491
                     /locus_tag="ACHUPB_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR0760292.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAZOZD010000221         6528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOZD010000221 DAZOZD010000000
VERSION     DAZOZD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.223"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..957)
                     /gene="rfaC"
                     /locus_tag="ACHUPB_003572"
     CDS             complement(<1..957)
                     /gene="rfaC"
                     /locus_tag="ACHUPB_003572"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0760293.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEEN"
     gene            complement(961..2007)
                     /gene="rfaF"
                     /locus_tag="ACHUPB_003573"
     CDS             complement(961..2007)
                     /gene="rfaF"
                     /locus_tag="ACHUPB_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0760294.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2017..2949)
                     /gene="rfaD"
                     /locus_tag="ACHUPB_003574"
     CDS             complement(2017..2949)
                     /gene="rfaD"
                     /locus_tag="ACHUPB_003574"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0760295.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3163..4359
                     /gene="kbl"
                     /locus_tag="ACHUPB_003575"
     CDS             3163..4359
                     /gene="kbl"
                     /locus_tag="ACHUPB_003575"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0760296.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4369..5394
                     /gene="tdh"
                     /locus_tag="ACHUPB_003576"
     CDS             4369..5394
                     /gene="tdh"
                     /locus_tag="ACHUPB_003576"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0760297.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            5633..>6528
                     /gene="waaH"
                     /locus_tag="ACHUPB_003577"
     CDS             5633..>6528
                     /gene="waaH"
                     /locus_tag="ACHUPB_003577"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR0760298.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1549 a         1633 c         1692 g         1654 t
ORIGIN      
        1 gttttcttca atgaatcgct taacagcatt tgctgtcaat tgagacaact cattccccgg
       61 agccctacaa accatctgat tcttcccata tccaccaatt aatcccggat cggttggtcc
      121 ataaaccgtg atattgggtc tatccagtgc cgccgttaaa tggcttaacc ccgtatccac
      181 cgacactaca aatttagccc cggccagcac gcgggcaacg ccttccagac tcatcttcgg
      241 caatacttca acataagcaa atccttccgc cagtcgtttc gcccgctctt cctcatgcgg
      301 cgcgccccac ggaagtttaa tccgtattcc tgaatcagcc agtaaaccaa tcaattctcg
      361 ccagtgttct tccggccagt gtttatcatc acgggtcgtc gcatgaagaa atacggcata
      421 ttcgccagca tctgtaggca ggttcgtcag gaaatgctgt gcaatagcat aatcgccctg
      481 ggtttgcggt ttgctatagc ccagactttt ggcaaacagt tcgcgggtgc gttctacggc
      541 gtgctgctgt tttgcaatat gatgcttacg attgtaaaac agactggcta aaggttcgcg
      601 agcggtttgc cagtccatgc catgctttac gccatgcgcc agacgcgtca ccagcgccgc
      661 gctttttacc agcccctgag cgtcgataac tgcgtcatag ttctctgctt gtagcgcttc
      721 acgaaacgct ttgcgttccg cttttatggg ggccgagaac caggctttac gccagcgacg
      781 tattgccaca ggaataactc gctcaacggc ggcgtgccag gaaggaatct gtgcgaaccc
      841 ttcttccacc acccagtcaa acttaatccc tgggattgcc tgctgggcat cagtgagtgc
      901 gggcaacgta tggagaacat cgcccatcga cgatgtttta acgatcaaaa cccgcatccg
      961 tcaggcttcc tcttgtaaca atagcgcgtt gagttcttcc agtacgcgct ggggagtaat
     1021 gtcgattaag ctctggtgat aaccctccgc agcgtcacct ttacgcactt tgtgatagcc
     1081 ggtaatcagg cggatcacgc gcgctttatg ggatagcggc ggtgtgaagt ccgggctact
     1141 cggaccatac agggcaacca gcggacgatt gagcgccgcc gcaacgtgca tcaggccaga
     1201 atcgttagtg acaatggctt tacaggctgc aatcaggata accgcttgat caagctgtgt
     1261 ttcccccgcc aggttccgac accatgcctg ctgctcggta ttcaaagcgg caagaatctc
     1321 attgcccgct tcatgatctt tcgccgagcc aaacagaacc acctgataac cttcatcaat
     1381 cagctgcttt gccagttccg catagtggta gtgtggccag cgttttgccg gaccaaactc
     1441 cgcacccggg caaaaaccaa tcatcggacg ttctgatgaa agcgaaaatt gattacaggt
     1501 atatgatttt tcaccttcgc tcacctgcaa ttgcggccat aacaacggtt ttggtagatc
     1561 ttgtgcggta cgcatcacgc ctttgtcata ggccagcgcg acatagcgtt ccaccattag
     1621 cggccaggct tctttatcga gcacgcgtaa atcgttgagt aaaccgtagc gcatctcgcc
     1681 gcgccagccg gtgcgatgag gaatacccgc gaagaaaggc actaatgcag atttgaagga
     1741 gttgggtaag acgtaggcgc ggtcgtagcg cttttcacgc aggctatgac ccagtttgcg
     1801 gcgttcgccg atttccagcg ctccgtgacc gagaggcata ggaatagctt cgttaacttc
     1861 cggcatccgc gataataatg gacggcacca tgccggtgcc atcacatcga ttatcgcctg
     1921 gggatagcgc gcctggagcg tgcgatagag actttgcgac atcatcatgt cgccaaccca
     1981 agacgggccg atcaccagta ttttcatgca gagctcttat gcgtcgcgat tcagccaggc
     2041 catgtattcc gttacacctt cagcaacggt tttgaacggt ttgtcgtaac ccgccgcgcg
     2101 cagatttgtc agatctgcct gagtgaacgc ctggtagcgg cctttcagct tatccgggaa
     2161 cggaatgtat tcgatctggc ctttcttgtg ataagccagc gtagcatcag ctacagcctg
     2221 gaaggattcc gcacgaccag taccgaggtt gaagatgccg gaaacgccat tttccaggaa
     2281 ccacagattc acatcagcca cgtcgcccac atagacgaag tcgcgtttga agttctcgct
     2341 accttcaaac agcttcggtg attcaccgtt gttaagctgg gtgttgagat ggaaagcgac
     2401 gctcgccatg ctgcctttat ggccttcacg cggtccataa acgttgaaat agcggaagcc
     2461 aacaatctgc gagttagctt caggcaggat ttggcgaaca tattcatcaa acaggaattt
     2521 tgagtaaccg tagacgttca acggtttttc gtactcgcgg gattcaataa agtcggaggt
     2581 gcgtccgccg taggtggctg cggaagaggc atacaggaac gggatttcgc gctccagaca
     2641 gtagtgcagc agctctttgg agtattgata gttgttatcc atcatatact tgccatccca
     2701 ctcggtggtg gaagagcacg cgccttcgtg gaaaatcgct tcgacatcgc cgaactcttc
     2761 gccagccata atctggatca ggaagtcttc cttatccata tagtccgcga tatccagatc
     2821 caccaggttc acaaacttgg tgccgtcttt caggttgtcc accaccagaa tatcggtgat
     2881 gcctttatca ttcagggcct taacgatgtt gctgccgata aagcccgcgc cgccggtaac
     2941 gatgatcata actgtaacct tcgaattatg gagtcagaga caatctcaga cacgaatact
     3001 tctatcatat cactacacgg cgttggcttc agccattcgc caaaagtgag attgatggac
     3061 gttatttatc ctgaattttg cagaagtgtt aacgcgtcat ctcgtcgcga cctataagtt
     3121 tgggtaatat gtgctggaat ttgccctgtc tggagaatcg caatgcgtgg agaattttat
     3181 cagcagttaa ccaacgatct ggaaaccgca cgggcggaag ggttgtttaa agaagagcgc
     3241 attattacgt ctgcgcagca agcagatatc actgtggctg atggaagcca cgtcattaac
     3301 ttttgtgcca ataactatct cgggctggcg aattatccgg atctgattgc ggcggcaaag
     3361 gcgggaatgg attctcacgg tttcggcatg gcttcggtgc gttttatttg cggcactcag
     3421 gacagccata aagagcttga acaaaaactg gcggcattcc tggggatgga agatgcgatt
     3481 ctctactctt cctgctttga tgctaacggt ggcctgtttg aaacgctgct gggcgcggaa
     3541 gacgccatta tctccgacgc actgaaccac gcgtctatta ttgatggtgt gcgtctgtgt
     3601 aaagctaaac gctatcgcta tgccaacaac gatatgcagg agctggaagc acgtctgaaa
     3661 gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca ccgatggtgt gttctcgatg
     3721 gacggcgtga ttgccaacct gaagggcgtt tgcgatctcg cagataaata tgatgccctg
     3781 gtgatggtag acgactccca cgcggtcggt tttgtcggtg aaaatggtcg tggttcccat
     3841 gaatactgcg atgtgatggg ccgggtcgat attatcaccg gcacgctcgg taaagcgctg
     3901 ggcggggctt ctggtggtta taccgcggcg cgcaaagaag tggttgagtg gctgcgccag
     3961 cgttctcgtc cgtacctgtt ttccaactcg ctggcaccgg ccattgttgc cgcatccatc
     4021 aaagtactgg agatggtcga agcgggtagc gagctgcgtg accgtctgtg ggcgaacgcg
     4081 cgtcagttcc gtgagcaaat gtcggcggcg ggctttaccc tggcgggagc cgatcacgcc
     4141 attattccgg tcatgcttgg tgatgcggta gtggcgcaga aatttgcccg tgagctgcaa
     4201 aaagagggca tttacgtcac cggtttcttc tatccggtcg ttccgaaagg tcaggcgcgt
     4261 attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa ttacgcgtgc agtagaagca
     4321 ttcacgcgta ttggtaaaca actgggcgtt attgcctgag gatgtgagat gaaagcgtta
     4381 tccaaactga aagcggaaga gggcatctgg atgaccgacg ttcctgtacc ggaactcggg
     4441 cataacgatc tgctgattaa aatccgtaaa acagccatct gcgggactga cgttcacatc
     4501 tataactggg atgagtggtc gcaaaaaacc atcccggtgc cgatggtcgt gggccatgaa
     4561 tatgtcggtg aagtggtagg tattggtcag gaagtgaagg gcttcaagat tggcgatcgc
     4621 gtttctggcg aaggccatat cacctgcggt cactgtcgca actgtcgtgg cggtcgtacc
     4681 catctgtgcc gcaacacgat cggcgtcggc gttaaccgcc cgggctgctt cgccgaatat
     4741 ctggtgattc cggcgttcaa cgccttcaaa attcccgaca atatctccga cgacctggct
     4801 tccatttttg atcccttcgg taacgccgtg cataccgcgc tgtcgttcga tctggttggc
     4861 gaggatgtgc tggtctctgg tgcaggcccg attggtatta tggcagcggc ggtggcgaaa
     4921 cacgttggtg cacgcaatgt ggtgatcact gatgttaacg aatatcgtct cgaactggcg
     4981 cgcaaaatgg gtatcacccg tgcggtcaac gtcgctaaag aaaaccttaa tgatgtgatg
     5041 gcagaactgg gcatggccga aggctttgat atcggtctgg aaatgtccgg tgcgccgcca
     5101 gcgtttcgta ccatgctcga caccatgaac cacggcggtc gcattgcgat gctgggtatt
     5161 ccaccgtccg atatgtctat cgactggacc aaagtaatct ttaaaggctt gttcattaaa
     5221 ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga tggcggcgct gattcagtca
     5281 ggtctggatc tctcgccgat cattacccat cgtttctcta tcgatgattt ccagaagggc
     5341 tttgacgcta tgcgttcggg ccagtccggg aaagtaattc tgagctggga ttaacacgaa
     5401 caagggctgg tattccagcc cttttgcctg aggataatcc gttaaatatg taaaatcctg
     5461 tcagtgtaat aaagatatcg taattgcgct gatctcttat atagctgctc tcattatctc
     5521 tctaccctga agtgactctc tcacctgtaa aaataatatc tcacaggctt aatagtttct
     5581 taatacaaag cctgtaaaac gtcaggataa cttcagaggt cgtcggtaat ttatgatgaa
     5641 cagcaccaat aaacttagtg ttattattcc gttatataat gcgggcgatg atttccgcac
     5701 ttgtatggaa tctttaatta cgcaaacctg gactgctctg gaaatcatta ttattaacga
     5761 tggttcaacg gataattctg ttgaaatagc aaagcattac gcagaaaact atccgcacgt
     5821 tcgtttgttg catcaggcga atgctggcgc atcggtggcg cgtaatcgtg ggattgaagt
     5881 ggcaacgggc aaatatgtcg cttttgtcga tgctgacgat gaagtctatc ccaccatgta
     5941 cgaaacgctg atgaccatgg cgttagagga cgacctcgac gtggcgcagt gcaacgctga
     6001 ctggtgtttt cgtgaaacgg gagaaacctg gaaatccatc cccagcgatc gccttcgctc
     6061 aaccggcgta ttaaccggcc cggactggct gcggatgggg ctttcttcgc gccgttggac
     6121 tcacgttgtc tggatggggg tttatcgccg cgatgttatt gttaaaaata acattaaatt
     6181 tattgccgga ttacatcatc aggatattgt ctggacaaca gaatttatgt ttaatgcgct
     6241 gcgtgcgcga tataccgagc aatcgttata taaatattat ctgcataata cgtcagtgag
     6301 tcggttgcat agacaaggga acaaaaacct taattatcaa cgtcactata ttaagattac
     6361 tcgtctgcta gagaaattaa atcgaaatta tgccgacaaa attacgattt atccggaatt
     6421 tcatcagcaa ataacttacg aagcattgcg tgtttgccat gcggtgcgca aagagccgga
     6481 tattattacc cgccaacgga tgattgccga gatatttact tccggtat
//
LOCUS       DAZOZD010000222         6521 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZOZD010000222 DAZOZD010000000
VERSION     DAZOZD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.224"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            72..758
                     /locus_tag="ACHUPB_003578"
     CDS             72..758
                     /locus_tag="ACHUPB_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0760299.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(849..985)
                     /locus_tag="ACHUPB_003579"
     ncRNA           complement(849..985)
                     /ncRNA_class="other"
                     /locus_tag="ACHUPB_003579"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1030..1117)
                     /locus_tag="ACHUPB_003580"
     tRNA            complement(1030..1117)
                     /locus_tag="ACHUPB_003580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1081..1083),aa:Ser,seq:gga)
     gene            1351..2289
                     /gene="ghrA"
                     /locus_tag="ACHUPB_003581"
     CDS             1351..2289
                     /gene="ghrA"
                     /locus_tag="ACHUPB_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR0760300.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2344..3081
                     /gene="ycdX"
                     /locus_tag="ACHUPB_003582"
     CDS             2344..3081
                     /gene="ycdX"
                     /locus_tag="ACHUPB_003582"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0760301.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3105..3659
                     /gene="ycdY"
                     /locus_tag="ACHUPB_003583"
     CDS             3105..3659
                     /gene="ycdY"
                     /locus_tag="ACHUPB_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0760302.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3761..4252
                     /locus_tag="ACHUPB_003584"
     CDS             3761..4252
                     /locus_tag="ACHUPB_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0760303.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4317..5150)
                     /gene="csgG"
                     /locus_tag="ACHUPB_003585"
     CDS             complement(4317..5150)
                     /gene="csgG"
                     /locus_tag="ACHUPB_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR0760304.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5177..5593)
                     /gene="csgF"
                     /locus_tag="ACHUPB_003586"
     CDS             complement(5177..5593)
                     /gene="csgF"
                     /locus_tag="ACHUPB_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0760305.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5618..6007)
                     /gene="csgE"
                     /locus_tag="ACHUPB_003587"
     CDS             complement(5618..6007)
                     /gene="csgE"
                     /locus_tag="ACHUPB_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0760306.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6012..>6521)
                     /gene="csgD"
                     /locus_tag="ACHUPB_003588"
     CDS             complement(6012..>6521)
                     /gene="csgD"
                     /locus_tag="ACHUPB_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0760307.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         1627 a         1500 c         1620 g         1774 t
ORIGIN      
        1 ggcatgtaac acgctgcagc gagttacatt ggtaatgaaa tataatcaac atggataggt
       61 gaaaattaat tatgagcttt atggcttttg taacttgttt gacaattgca tttatcgccg
      121 gttttgctgt tgccagaaaa ttgacactcc gaaaagttgc ctcatcggta atcatcgcta
      181 aatcgcaacc cgtcccttca tctgcaactt cccctgccgc taaggcgtcg acaaggagct
      241 atataccgca gaaaacctat cgtgactctc gttggcaaga gcgagatgag ttatttcagg
      301 gaatggttgc cgaaggtatt ttattggaaa gaaaagagtg gttaatcacg cgttgtgaaa
      361 aaatgtattt cgataatctg gaaaaatggt ttggacatta ttgtcatatc cattgccagg
      421 tctctctggg gcgcttgatc aactttccag aacaacctga gtttagcgaa gaagaaagaa
      481 agcgtttctt tgcaatttat aatggcatgg caatggatta tgttctggtt tcaaagaaga
      541 acaataaaat agtctgtgtt attgagctgg atgacccgag tcatgaatgg gcggatcgca
      601 ttattcgtga ccgttggttg gatacaatta tgactcttgc acgggtgcct tttgtacgag
      661 cttctgtcac taaattggag gaaattcctc cggtctggga gactcgtgac aaaattcgcc
      721 cattagtcgc ggcgacaaag agtgaaccgg tgcattaact aagtatcccg gtcggggctt
      781 ctcatccccc cggtgtgtgc aatatacgaa aaaaagcccg taccttcgta cgagctcttc
      841 tttaaagatg gcggtgaggg ggggattgac tcgcttcgct cgccctgcgg gcagcccgct
      901 cactgcgttc acggtctgtc caactggctg tcgccagttg tcgaaccccg gtcggggctt
      961 ctcatccccc cggtgtgtgc aatatacgaa aaaaaagccc gtactttcgt acgagctctt
     1021 ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt
     1081 tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc tgtcttgctg
     1141 gaacgggcgc taatttaggg aaatcatgac ctgaggtcaa caaacttttt gaaaaaatcg
     1201 cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa gcgaaatctg ctttttttgc
     1261 caccgaccac ggatttgtta tgctggtggc ctttgtagat cataacgata agtgcgaata
     1321 aatttcgcac aacgcttttc gggagtcagt atggatatca tcttttatca cccaacgttc
     1381 gatacccaat ggtggattga ggcactgcgc aaagctattc ctcaggcaag agtcagagca
     1441 tggaaaagcg gagataatga ctctgctgat tatgctttag tctggcatcc tcctgttgaa
     1501 atgctggcag ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt tgattctatt
     1561 ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt ctgttccact ttttcgcctg
     1621 gaagataccg gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt gctgcattgg
     1681 tttcgacgtt ttgacgatta tcgcatccag caaaatagtt cgcattggca accgctgcct
     1741 gaatatcatc gggaagattt taccatcggc attttgggcg caggcgtact gggcagtaaa
     1801 gttgctcaga gtctgcaaac ctggcgcttt ccgctgcgtt gctggagtcg aacccgtaaa
     1861 tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac tgtctgcatt tctgagccaa
     1921 tgtcgggtat tgattaattt gttaccgaat acccctgaaa ccgtcggcat tattaatcaa
     1981 caattactcg aaaaattacc ggatggcgcg tatctcctca acctggcgcg tggtgttcat
     2041 gttgtggaag atgacctgct cgcggcgctg gatagcggca aagttaaagg cgcaatgttg
     2101 gatgttttta atcgtgaacc cttaccgcct gaaagtccgc tctggcaaca tccacgcgtg
     2161 acgataacac cacatgtcgc cgcgattacc cgtcccgctg aagctgtgga gtacatttct
     2221 cgcaccattg cccagctcga aaaaggggag agggtctgcg ggcaagtcga ccgcgcacgc
     2281 ggctactaat aaagcatcag gattcctgct atccttggcg ggaattgaat acaggagaga
     2341 gttatgtatc ccgtcgacct tcatatgcat accgttgcca gcacacatgc atatagcaca
     2401 ttgagtgatt acattgccca ggccaaacaa aagggcatta aactttttgc gatcaccgat
     2461 catggcccgg atatggaaga tgctccgcat cactggcact tcattaacat gcgtatctgg
     2521 ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg aagctaacat taaaaatgtt
     2581 gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc tggatctgat tattgccggt
     2641 tttcatgagc cggtttttgc accacatgac aaagcgacca atacacaagc gatgatcgcc
     2701 actatcgcca gcggcaatgt gcatataata agccatcccg gaaatcccaa atatgaaata
     2761 gatgtgaaag ccgttgctga agcagccgcg aaacatcagg tggcgctgga aatcaataat
     2821 tcttcatttt tacactcacg taagggcagt gaagacaact gtcgtgcggt agccgcagcg
     2881 gtacgtgatg ctggaggttg ggtggcatta ggctcggatt ctcacaccgc gtttaccatg
     2941 ggggaatttg aagagtgtct taaaatcctc gacgcggtag attttccgcc agagcgcatt
     3001 ttgaatgttt ctccgcgccg cttactgaac ttccttgaat ctcgcggtat ggcaccgatt
     3061 gcggaatttg cagaccttta attactagta atggaaatat attaatgaac gagttttcta
     3121 tcctctgtcg tgtgctgggt tcgctctatt accgccaacc gcaagatcct ttactggtgc
     3181 cgctgtttac cctgattcgt gaggggaaac tggctgcgaa ctggccactg gagcaggatg
     3241 agttactgac gcgtttacag aaaagctgtg atatggcgca agtctctgcc gattacaatg
     3301 cgttgtttat cggcgatgaa tgtgcagtgc cgccatatcg tagcgcatgg gttgaggacg
     3361 taacggaagc ggaagtgcgc gcttttcttt ccgaacgagg gatgccactg gcggatacgc
     3421 cagccgatca catcggcaca ttgctgcttg cggcttcctg gctggaagat cagtcaacgg
     3481 aagatgagag cgaagcactg gaaacactgt tcagtgagta tctgttaccc tggtgtggtg
     3541 cgttcctcgg caaagtggag gcccatgcaa ccacgccttt ctggcgcacc atggcaccgc
     3601 taacccgtga tgccattagt gcaatgtggg acgagctgga agaagattct gaagagtaaa
     3661 tgtgatcatc gtcactcata actgcaacag ataatttgtt gttgcataaa atgtgtgctc
     3721 gatctcattc atggccgcgt tttctgctat catgcgcggc atgaacatac ttctctctat
     3781 tgcaatcaca acaggcattc tctccggtat ctggggatgg gtggctgttt ccctgggctt
     3841 actaagctgg gcgggcttct tgggctgtac ggcctacttt gcctgcccgc aaggtgggct
     3901 gaaagggctg gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg cgatggtcat
     3961 tatttacggt agtgcactgg caccacatct ggaaattctc ggttatgtca taaccggtat
     4021 tgtggcgttt ctgatgtgta ttcaggccaa acagctgctg ctttcatttg ttccggggac
     4081 gtttataggc gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg tgttaccttc
     4141 gctggcgctt gggctggtat ttggttacgc aatgaaaaac agcggcctgt ggctggcggc
     4201 gcgtagtgca aagacggctc accgtgagca ggaaatcaaa aataaagcgt gaggggcact
     4261 cacgctttcg cttaaacagt aaaatgccgg attattaatt ccggcatttt tatctgtcag
     4321 gattctggtg gaaccgacat atggcggtat ttaaccagaa tgtcattctg ccgttctgct
     4381 ttgttctgca aatcccacag accacggtcg ataccatcat taatcaggaa aatgacccct
     4441 gtttcgatag ccgacatcag acacagcata acgggttcgt ttgaggtgta acccacttcc
     4501 ccttccagca agcgctggta gtcaataaag cggaaaaccc cggcctgaac ttcataggaa
     4561 agtatcgtct tactggtgtt caccgaggag aggatctcac cggtactcac attgacgaca
     4621 cgcaggttca cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat cccaaaatat
     4681 cttgccccaa caccgcccga tttaacgttg ctttcataac cgataatcga accttcaacc
     4741 atgatatttg ccgccgttaa agattgcagc gggattcggt tattaatggc aactgtgccg
     4801 ttttcttgtg ccgcacgaat aatcttgcgc tcgttaagca ggttttgtaa actctgacgc
     4861 tccagcggta taaaccagcg agaatctttc aatgccgtga ccagcattgc cgtggcgctt
     4921 tgcggaacag cagtggagaa gttacttgcc gggtagggtt taaattgccc ggtttcgtcc
     4981 tgaatgttgt ataccgaaac aaagatctta cccgtcggcg ctggcagatg ggtcaaatct
     5041 ttgtagctct gagctcgagg cattaatgtc ggtctggcgg cttctttagg cggggcggtt
     5101 aagcatccgc tcagtaacat gacggcaacc aaaagaaata agcgctgcat gattattttc
     5161 cttatgaagc tggggcttaa aaatcggttg agttattttg taaacccgaa acctggatgg
     5221 tcgaggtttg tccggtttta cgatctgtca cgttcaactg caattgacca tcgcggttag
     5281 caatatcaac aatataatcg ttggtcacca tgcggcccgg tttaccggta ttaatattcg
     5341 acagtagccc acctaaaatt tgtgactgga tggcctgagt aaagttatct aacgctgagg
     5401 gtgtttcaat accaaagtca tcgttatagc tcggatcttt ataagagttt tgggcctgag
     5461 cgctatttaa taaaaaagcg ccattatttg ggttaccacc aaagtttgga ttacggaact
     5521 ggaaagtcat ggttccagcc caacttaatg gcgaaataag catgagtaga actactgcat
     5581 gtttgacacg cattgcagcc tccgaacaat tttttattta gaattcatca tgcgccaaat
     5641 cgcccgtact taataacgcc tgatttatct ggcgacgatt tagtgcttct tcagtttgaa
     5701 tcagtgcaaa gacgacagtt ttctcgaagt ctcttttcaa cggaaataaa aaagtctgga
     5761 aaataacgtc ctgattgacc gttatagtga tccagcttcc ccatcgtgca ctgggccttt
     5821 cattaatcgt taagttaccc gtatagtcac tttcccattt atcactaaag gctcggtaaa
     5881 aatcatggcc aatagatgaa acagtatggt cagttagcaa tcccgggact tctacctcaa
     5941 cggcgtgaag attccctgcg gcgaacagaa attctgccgc cacaatccag cgtaaataac
     6001 gtttcatggc tttatcgcct gaggttatcg tttgcccagg aaaccgcttg tgtccggttt
     6061 tttacggcta tcttcttgaa aagattataa agatgcgttt taaccgtatt ttcgctgatg
     6121 aacaacgaac gagcgatctc gttattagac gcgccgatac gcagtttatt caggatctct
     6181 ttttcccgat gagtaaggag ggctgattcc gtgctgttat aacgatagtt acctgaatgc
     6241 gtaatcaagt agctggcaag cttttgcgta aagtagcatt cgccgcgcag gacgccttgc
     6301 aacccattga caacacgttc ttgatcctcc atggcataaa aaacgccgtt gatatgaggc
     6361 cagttttcaa tgtcgcggta cgggtaatct tcaggcgtat ttagcaacaa tattttgata
     6421 ttgttgtttt tcctgctcaa agtatcctgc caataatgga taagcttttt atccgcttcc
     6481 atcatatcca gaagaataat agagcctgaa gatatatcgt c
//
LOCUS       DAZOZD010000223         6515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOZD010000223 DAZOZD010000000
VERSION     DAZOZD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.225"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUPB_003589"
     CDS             38..250
                     /locus_tag="ACHUPB_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760308.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUPB_003590"
     CDS             283..501
                     /locus_tag="ACHUPB_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0760309.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUPB_003591"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUPB_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUPB_003592"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUPB_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0760310.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUPB_003593"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUPB_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUPB_003594"
     CDS             1917..2660
                     /locus_tag="ACHUPB_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0760311.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUPB_003595"
     CDS             2690..3229
                     /locus_tag="ACHUPB_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0760312.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUPB_003596"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUPB_003596"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0760313.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUPB_003597"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUPB_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0760314.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUPB_003598"
     CDS             4582..6102
                     /locus_tag="ACHUPB_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0760315.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="ACHUPB_003599"
     CDS             complement(6135..>6515)
                     /locus_tag="ACHUPB_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760316.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAZOZD010000224         6500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOZD010000224 DAZOZD010000000
VERSION     DAZOZD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.226"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUPB_003600"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUPB_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0760317.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUPB_003601"
     tRNA            1230..1305
                     /locus_tag="ACHUPB_003601"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUPB_003602"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHUPB_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUPB_003603"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHUPB_003603"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0760318.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUPB_003604"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHUPB_003604"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0760319.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUPB_003605"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHUPB_003605"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0760320.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHUPB_003606"
     CDS             complement(4869..5306)
                     /locus_tag="ACHUPB_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR0760321.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="ACHUPB_003607"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="ACHUPB_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
BASE COUNT         1739 a         1756 c         1422 g         1583 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa
//
LOCUS       DAZOZD010000225         6494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOZD010000225 DAZOZD010000000
VERSION     DAZOZD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.227"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHUPB_003608"
     CDS             <1..153
                     /locus_tag="ACHUPB_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0760322.1"
                     /translation="LRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQGS"
     gene            182..346
                     /locus_tag="ACHUPB_003609"
                     /pseudo
     CDS             182..346
                     /locus_tag="ACHUPB_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            complement(384..1445)
                     /locus_tag="ACHUPB_003610"
     CDS             complement(384..1445)
                     /locus_tag="ACHUPB_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0760323.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1658..3121
                     /gene="bepA"
                     /locus_tag="ACHUPB_003611"
     CDS             1658..3121
                     /gene="bepA"
                     /locus_tag="ACHUPB_003611"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0760324.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            3142..3501
                     /gene="arsC"
                     /locus_tag="ACHUPB_003612"
     CDS             3142..3501
                     /gene="arsC"
                     /locus_tag="ACHUPB_003612"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0760325.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3639..4340)
                     /locus_tag="ACHUPB_003613"
     CDS             complement(3639..4340)
                     /locus_tag="ACHUPB_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0760326.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="ACHUPB_003614"
     CDS             complement(4435..5724)
                     /gene="uraA"
                     /locus_tag="ACHUPB_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0760327.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5810..6436)
                     /gene="upp"
                     /locus_tag="ACHUPB_003615"
     CDS             complement(5810..6436)
                     /gene="upp"
                     /locus_tag="ACHUPB_003615"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0760328.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
BASE COUNT         1717 a         1774 c         1625 g         1378 t
ORIGIN      
        1 ctcagggatc cgctctccag ggcttactac acccgcaaaa tgagtcaggg aaaacgacac
       61 aatcaggcgc ttatcgccct ggcgagacga cgctgcgacg ttctgttcgc catgatgcgc
      121 gacgggactt tttatacccc gcaggggtca taacatgctt gacaacttaa taggggcacc
      181 ccccttctgg caactggcgc acagtagcgc agacaatttt ccggcactga cggtaagcca
      241 ttttattacc gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct
      301 ggtgagtatg tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc
      361 gaaacagttc gtcgggctga agattattct tgcgctattt gcccatcggg ccaggcgtgg
      421 atcacggctt tgatcagcgt cgccaatgga atagcgaaga atacgcccca gaatccccac
      481 aaaccaccga agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg
      541 gagaacaaca ccggtactaa cagattaccg tccagcgcct gaataatcag atacactgcg
      601 aagcagctcc agaattccgt gcctgcacca aactggaata gcgccacgcc aaccaccgga
      661 atggtcacca caaatgcgcc aatgtacgga ataagtaccg agaagccgac cagcaccgcc
      721 agcagcagcg aatagttcag cccaaagagc aagaacccca gccaagtggc gatccccacc
      781 acgatcatct ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc
      841 cacacctgtc ctgccagtcc acggttgcgc ggcagcaccc gacgaacggc gttcagcatc
      901 tgctctttgt ctttcagcag gaagaagacc atcaatggta ccagcaccag atagacggca
      961 atggtcagca aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc
     1021 agcatccgac tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaatgcc
     1081 ggatagcggc gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata
     1141 tcgcggatca agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt
     1201 agcaatatac cgacgaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca
     1261 atagattgca ggcgcacggt tggccattcc agcaaatagg ccagcacaat agccaccagc
     1321 aacggagcaa gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc
     1381 agcaaggcaa tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg
     1441 agcataaaga ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat
     1501 gtcacaaaaa agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg
     1561 ggctaatctt cgccgttaca ctcaaaggcg gcgcggtggg aacaatattt cacagtatcg
     1621 gtcaaatgac tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa
     1681 cctggttgca accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga
     1741 cagcgcagac accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca
     1801 ggaaatgcag atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa
     1861 tgacccgctg ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa
     1921 ttcggttaag acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc
     1981 tttctttggc ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag
     2041 tcaactggct tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg
     2101 agcgatggaa gatcagcagc gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat
     2161 tttactggcg atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg
     2221 aacgcgtcag gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg
     2281 tattcaggtg ctgcaacgct cgggattcga tccgcaggcg atgccaacct tcctcgaaaa
     2341 attactcgat caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt
     2401 gccggaaagt cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt
     2461 gcagtcgtcg gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg
     2521 acgtaaccag ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca
     2581 acgagcggcg caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc
     2641 gcgtaaaacg ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct
     2701 ggctaccgat atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa
     2761 tgcccgtgat ttgcgcacca atccggtgtt gcagctcaac ctggcaaacg cttatctgca
     2821 aggcggtcaa ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga
     2881 tgacagcaac ggctgggatt tactggcaca ggcggaagcc gcgctaaata accgcgatca
     2941 ggagctggct gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc
     3001 gctgttgagt agcgccagtt cgcaggtgaa attaggcagc cagcaacaag cgcgttacga
     3061 tgcgcgcatc gaccagttgc gccagctgca ggaacgcttt aagccttata ccaagatgta
     3121 attcgcatta aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt
     3181 tcaaagagcc gggaaacgct gaacctgctg aaaaaaaacg gcgtggagcc agaagtggta
     3241 ctctaccttg agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg
     3301 atgaatagcg cccgtgaatt gatgcgccag aaagaggatc tttataaaga actgaacctg
     3361 gctgacagtt cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg
     3421 atggagcgcc cgattgtggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag
     3481 gtactggaaa tcgtgggtta agatgtgccc catcgctagt tgaagcacat tgccggatgc
     3541 gacgcttgcc atgtcttatc cggcctacga atactatgga ttaacttcgc gtagttcgga
     3601 taaggcgttc gcgccgcatc cgacaataaa caccttatct acaacttcag aatttctttc
     3661 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccaa ctgatccaac
     3721 gtcataaata gcgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
     3781 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
     3841 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gtcgcgacgc gagatccggt
     3901 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaatcg tgttttgccc
     3961 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
     4021 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
     4081 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgcgac
     4141 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
     4201 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcagccag taaagaggag
     4261 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
     4321 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
     4381 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
     4441 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcacactaat
     4501 cagtttgaag atcaaactta agccgatacc gacgatggtc gccagcgcca tacctttcag
     4561 ctccgccgcg ccaatgttta ccttcgcgcc actgacaccg atgatcaaaa tcacggaagt
     4621 caggatcagg ttctgtgctt tgttgtaatc cacttttgat tcgatcaaaa cacgaatacc
     4681 ggaagcaccg atgacaccat aaagcagcag cgaaacgccg cccatcaccg gcaatgggat
     4741 catctggata gcggcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
     4801 cccaccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
     4861 gtaagtggta tttggcgtag agccaaagaa gccggagatc acagtcgaca ggccgttagc
     4921 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
     4981 tactaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
     5041 aatggcaaac cactcgaagc gcggcgtata gagggttggc agcgcaaacc agtgagcatt
     5101 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtacc ccaccagcac
     5161 gccaattaaa atcgggataa ttgccaggaa accacggaac agcacggaac ctaaaaccgt
     5221 gaccgccagg gtggtaatag agatgatgat ggttttggag tctggcgttt gcccttcagc
     5281 cgggagtaaa cccgccatac cggcagctac gcccgccagc tccagaccga tgacggcaac
     5341 gattgcgccc attgccgcag gtggaaacag cacgtccagc cagccggtcc ccgctttctt
     5401 cacgataaaa gaaaccaggc agaacagcac gccgcacata ataaagccgc ccagcgcgac
     5461 ttcataccct aacggcaaca gtaacaatac cggtgaaata aaggcaaagc tggaaccaag
     5521 ataagccgga attttccctt tacagatgaa gagatacagc agcgttccaa taccgttaaa
     5581 taacagtaca gtcgccgggt taatatgaaa taagacgggc accaggacgg ttgcaccaaa
     5641 catggcgaac aaatgttgca aactaagcgg gattgtctgt aaaagtggcg gtctttcact
     5701 caccccgata gcacggcgcg tcatagtatt atcctctgta ttatgtgtta taggcgcttt
     5761 actcaaaaaa aagccgactc ttaaagtcgg ctttaattat ttttattctt tatttcgtac
     5821 caaagatttt gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc
     5881 cctggtcaat cgatgcggta tacagttcga cgtccgggtg cgctttttcc agcgcagcga
     5941 taccttctgg cgcagctacc agcaccagaa ctttgatgct gctgcagccc gcttttttca
     6001 gcaggtcgat ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg
     6061 ccatacgctc atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt
     6121 cttcattacg gtacataccg acaacgctga tgcgcgcgct cggaacattt tccagcacac
     6181 cgtccatcat ccccagaccg gcacgcagaa ttggcacaac ggtaattttc ttacctttga
     6241 tctggtcgat ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt
     6301 cggcggtcgc ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct
     6361 tggtgctgat atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga
     6421 cttccacgat cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat
     6481 tatacctcct ttct
//
LOCUS       DAZOZD010000226         6490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOZD010000226 DAZOZD010000000
VERSION     DAZOZD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.228"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..163
                     /gene="ymgJ"
                     /locus_tag="ACHUPB_003616"
     CDS             <1..163
                     /gene="ymgJ"
                     /locus_tag="ACHUPB_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0760329.1"
                     /translation="TITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTAFKKCLF
                     LMRDIIISS"
     gene            124..243
                     /locus_tag="ACHUPB_003617"
     CDS             124..243
                     /locus_tag="ACHUPB_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0760330.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(722..988)
                     /gene="minE"
                     /locus_tag="ACHUPB_003618"
     CDS             complement(722..988)
                     /gene="minE"
                     /locus_tag="ACHUPB_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0760331.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(992..1804)
                     /gene="minD"
                     /locus_tag="ACHUPB_003619"
     CDS             complement(992..1804)
                     /gene="minD"
                     /locus_tag="ACHUPB_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0760332.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1828..2523)
                     /gene="minC"
                     /locus_tag="ACHUPB_003620"
     CDS             complement(1828..2523)
                     /gene="minC"
                     /locus_tag="ACHUPB_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0760333.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3043..3411
                     /locus_tag="ACHUPB_003621"
     CDS             3043..3411
                     /locus_tag="ACHUPB_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0760334.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3519..3920)
                     /gene="pliG"
                     /locus_tag="ACHUPB_003622"
     CDS             complement(3519..3920)
                     /gene="pliG"
                     /locus_tag="ACHUPB_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0760335.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4162..4455
                     /locus_tag="ACHUPB_003623"
     CDS             4162..4455
                     /locus_tag="ACHUPB_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0760336.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4527..5186
                     /locus_tag="ACHUPB_003624"
     CDS             4527..5186
                     /locus_tag="ACHUPB_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0760337.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5278..5724
                     /locus_tag="ACHUPB_003625"
     CDS             5278..5724
                     /locus_tag="ACHUPB_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0760338.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5931..>6490)
                     /gene="hlyE"
                     /locus_tag="ACHUPB_003626"
     CDS             complement(5931..>6490)
                     /gene="hlyE"
                     /locus_tag="ACHUPB_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0760339.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1660 a         1449 c         1536 g         1845 t
ORIGIN      
        1 aacgattaca ttagtcattt tattttgccg acggcctcat tgtcgaaaga taagcgtacg
       61 acagtattat ctgaaaagag tgatttttta tccaactaca cttcagcgca ctgcgtttaa
      121 aaaatgcctc tttcttatgc gggatatcat catttcatca tgatgtcttt ggtgagcggt
      181 gaacacaata cacctgcgct gtctcttcag gatgaatccc ctggtctggt gcctgcgggc
      241 tgatgttgca gcagagctta ggtcacttag acgctactat catttatcca atggcatgga
      301 atcgaaatca gtcgataccc gcagtatata tcgtgaactg gtcgttctga ccatcaacga
      361 ttaaactgcg cttcggcgca gttttcgttt acagggggct aaaagggaaa attctggggc
      421 aaaaaaagcc cgccagttac ggcgggaaac ctcatcctat gggagaacaa tgaataatga
      481 aattgcgggg ttatcatccc ccagtgtatc cacactaaca ataaggttat ttactcagcc
      541 aggcataaac attatgttct gtgcgtggga acagccttaa ggtgtaaagg gggaggtgga
      601 aatagcaatg aggagtatca gcaagaatac tcgccgcttt accacaacgt ggatgagagg
      661 gatgaaaaac tcaaggcaga aatgactctg ccttgaagat aaatgcgttt ttacagcggg
      721 cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata gaaatatcgc
      781 catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtacg tatttacaaa
      841 tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca tcgctgcgac
      901 ggcgttcagc aacaataatc tgcagccgtt cttttgcaat gttggctgtg tttttcttcc
      961 gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt ttgaggaagc
     1021 ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga cgttctacgg
     1081 tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt tcaccctggt
     1141 tagaggcgcg taatactgat tgatcctctg ggatcacgcc gacgagtttg atgcgcagga
     1201 tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct gggttatagc
     1261 gcgttaacag caggtgctct ttaataggct cttcgccatt ttctgcgcgg cgtgatttcg
     1321 acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc gggttggtgg
     1381 taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca atccctgccg
     1441 gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga actttggcga
     1501 ccccttcacg ggtgagggca tctttatcgc gtgtttgcga tgccggaaga atatagagat
     1561 tttcagtacg cttatcttta attaacgcct gatttagcgt tgcatcgccc tgaatgacgt
     1621 tgacgaaatc gtaaacgacc cggcgttcgc aacccataat caggtcgaga ttacgcaggc
     1681 cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca gtggcgatgg
     1741 ccgcgctgga ggttgtctta ccaacccccc ctttgcccga agtaacaaca ataatgcgtg
     1801 ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca aagcgttttc
     1861 gactaactgc agtcgcgccg ctttgccata aaattctgct gggatttgat cactcagcca
     1921 gtattcacct gcgatggaca ccaattccgc catcaggttc gtacaaaata tttgcgtttc
     1981 ccggtcacca cttgcccctg ccagcgcacg accgcgcatc atgccataga catgaatgtt
     2041 cccatcggca atcaattcgg ccccagcgct aacgtggctt gtaacaatca gatcacattg
     2101 tggagcataa atacgctgac cggaacgcac cggggtatct attaaacgcg tttttgtgac
     2161 cggcgtggta ttttgcgctg gagcctgtgg tgcgggagct ggacgtggcg ctttttcctt
     2221 tccttccgtc aggataggca gccccatttt ttcaatttcg gctttaagtt gcgcgtcttt
     2281 gcagccactt acgccaataa cccgcaaacc ggttgccgaa accgccttat gcatcgctga
     2341 ccagtttacc gggtcttcca gtgcactgac gttgagtaca acgggggcat gttttaaaaa
     2401 tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag gttctgcctc
     2461 atgcagatga accacagata aagtgaagct actgccttta agctcgattg gcgtgtttga
     2521 catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat gttccgaaga
     2581 ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca tttattcaga
     2641 gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata cccttgtcaa
     2701 tcaacccatt gccgccgtac ttttgattct tcttatttac gcttcctttt tccgcaccct
     2761 aatcgtgaca gcatctccag acctgtttaa atatccagca ttgaggcaag tgtatttttt
     2821 caccacgcca ggctttctta actttccatt ttttgcagca tcattaaaga acagataccc
     2881 gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag acgtctgata
     2941 cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga ttgatgacga
     3001 aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat gatgcgtatt
     3061 atttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc tggacatgac
     3121 gctggcggac ttcaatctcc ggcgtgcggc gttgtttgcg acccgtatat ttgtgtgaac
     3181 tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac
     3241 ttacaatcat tacaaggcta cgatcccagc gaatttacat tcgctaacgg tgtattttgc
     3301 gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtagatgg taagcggagt
     3361 ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg atgccttgtt
     3421 tacattatgt tctctgctat tcgtcattaa cctcttgagc gacagagagg agtggggcgt
     3481 taacgtctat gatggggata gtccccgtcg gttgacattt acttaatttg aatatcgacg
     3541 ttatattttt tagttttgtt tttacgggcg tcgtttcgag tttgcagcac tcgcagctca
     3601 tatttgccag aagcagggag tgagtattgt ccgtgactat cgagttctgg ggaatatctg
     3661 gataaatcaa cagaatcatc gattcctggc ccgaagagat aagtatcagc gccttcatta
     3721 gagatactca catgcacttt ctgacctttt ttggcataga aggtatatgt atcgtaatcg
     3781 tatcccttta tttcgccgga gtactgagcg ctgctatggc ctttgctgaa ctcaacattg
     3841 acatttttac ccgcagcaaa gctcgtggag gttagcagtg caagcagcaa tacagcctta
     3901 ctgatgctct tgattttcat tggtagtctc ctgttcagaa tgagtacgat gaactgttat
     3961 aatataacaa tcccttacgg gagaattcca gtggcttttc tgcgttcggt aaaatcccgg
     4021 cgcggcattt tttgccgggg ggattactta taaaggggca tggactcaac aaaccgcaga
     4081 aatctacagc tggttggctt gtggcgcggg tttcattgat gttagcttat gccaaaacct
     4141 ggcatcttaa agagtaaaag tatgttttgt gtgatttatc gaagcagcaa gcgtgaccag
     4201 acctatttat atgtcgaaaa aaaagacgat ttttcgcgtg ttcctgagga actgatgaaa
     4261 ggttttggtc agcctcagtt agcgatgatt ctgccgttgg atgggcggaa gaaactggtc
     4321 aatgctgata ttgaaaaggt taaactggca ttaaccgagc aaggttacta tttgcagtta
     4381 ccgccaccac ctgaagattt gctgaagcaa catctttccg tcatggggca gaaaacagac
     4441 gacactaaca aataaccgat atccggcggt ggcattatct ttgtcggcgc gggttttcat
     4501 atccacgata aggtgagggg aacgttatgt atcaacatca caactggcaa ggtgcgctgc
     4561 tggattatcc ggtgagtaaa gtagtctgtg ttggcagtaa ctatgccaaa catattaaag
     4621 agatgggcag cgcagtgccc gaagagccag tgctgtttat taaaccagaa acggcactgt
     4681 gcgatctgcg gcagccattg gcgatcccat ccgatttcgg ttcagttcat catgaagtcg
     4741 aactggcggt gttgattggc gcgacgctgc gtcaggcaac ggaagagcat gtccgcaaag
     4801 ccattgccgg ttacggcgtg gcgcttgatc tgacattgcg tgatgttcag ggaaaaatga
     4861 agaaagccgg gcagccgtgg gaaaaggcta aagcgtttga taactcttgt ccgctttccg
     4921 ggtttatctc cgcggcggaa tttaccggcg atccacaaaa tacaacgctg ggcctgagcg
     4981 taaacggcga acaacgccag caaggtacga ctgcggacat gatccataaa atcgttccgt
     5041 tgatcgctta tatgagcaag ttttttaccc tcaaggccgg tgacgttgtg ttgacaggca
     5101 cgcctgatgg cgtcggcccg ttgcaaagcg gtgatgagct gacagtcact ttcgatgggc
     5161 attctttgac aactcgcgtt ttgtaatact ttttgccgcc tgaaagcggc ggcaaaactt
     5221 gcatcgctgt gccagactgg ttataaggtg cgttttaacg taatggcgga acacctgatg
     5281 agcgatgtac ctttctggca aagtaaaacc ctggacgaaa tgagcgatgc ggaatgggag
     5341 tcgttgtgtg atggttgcgg tcagtgttgc ctgcataaac tgatggatga agacaccgac
     5401 gaaatctact tcactaacgt cgcctgtcgc cagctcaata ttaaaacttg tcaatgtcgg
     5461 aactacgaac gtcgtttcga gtttgaaccc gactgcatta aattaacccg tgaaaatctg
     5521 ccaacattcg aatggctgcc aatgatctgc gcttatcgtt tgctggcgga aggtaaagat
     5581 ttacctgcgt ggcatccgct acttactggt tcgaaagcgg caatgcatgg tgaacgtatc
     5641 tctgtgcgtc atatcgcagt gaaagaatca gaagtgattg actggcagga tcatatctta
     5701 aataaacctg actgggcgca gtgataaatt taatagatgt tgatgggggc tttcttaata
     5761 tacgtctgga tgctcgccat ttcgtagccc tgtttttgaa agcgctctgt cataatgaga
     5821 gttacccgat accttattat caacaggtaa ctctcaatct ttaaaaaacg tctgtgtttt
     5881 taaatgtgat aaaccttata ccttgagtac atggagagaa taatcgctga tcagacttca
     5941 ggtacctcaa agagtgtctt tttaccgtgt tttttctgat actcattaca ggtgttaatc
     6001 atttttttgg ccgcttcttt tagcaaagaa agcattaaat catcataatc aacgtagaat
     6061 ctggtggttt cagtttccgt ttttatctct ccgatggcgg ctatttcggt ggttaatttc
     6121 aatttggcgg catcgatatc tttattcgct tgtttaaccg tgttagacag ggtggtaaag
     6181 aaactctgca cagattttaa cttgttcttc aattctggga tcagtttccc ttcaactacg
     6241 cccgcagcaa tagaatagga aatgattaaa ccaaatggac cggcgacgac accggctgcg
     6301 gcaccggcat acgcttcctt cctgatttta tctacctgtg actggaaata gctgcttttt
     6361 tctgaaaaat cattggttaa ctggctatct aacgccagca gtttcccgga agcgttgttg
     6421 aaactttgtg agcttaccag cagggatttt tgcgcttcat tcagcttcgt gatgccgtca
     6481 tccagtacct
//
LOCUS       DAZOZD010000227         6392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOZD010000227 DAZOZD010000000
VERSION     DAZOZD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.229"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..357
                     /gene="ydjO"
                     /locus_tag="ACHUPB_003627"
                     /pseudo
     CDS             1..357
                     /gene="ydjO"
                     /locus_tag="ACHUPB_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(361..1752)
                     /gene="tcyP"
                     /locus_tag="ACHUPB_003628"
     CDS             complement(361..1752)
                     /gene="tcyP"
                     /locus_tag="ACHUPB_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR0760340.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1885..2475)
                     /locus_tag="ACHUPB_003629"
     CDS             complement(1885..2475)
                     /locus_tag="ACHUPB_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0760341.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2638..3306)
                     /gene="hxpB"
                     /locus_tag="ACHUPB_003630"
     CDS             complement(2638..3306)
                     /gene="hxpB"
                     /locus_tag="ACHUPB_003630"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0760342.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3453..3989
                     /locus_tag="ACHUPB_003631"
     CDS             3453..3989
                     /locus_tag="ACHUPB_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0760343.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4030..4890)
                     /locus_tag="ACHUPB_003632"
     CDS             complement(4030..4890)
                     /locus_tag="ACHUPB_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0760344.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4996..5286)
                     /gene="ghoS"
                     /locus_tag="ACHUPB_003633"
     CDS             complement(4996..5286)
                     /gene="ghoS"
                     /locus_tag="ACHUPB_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0760345.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5387..6316)
                     /gene="pfkB"
                     /locus_tag="ACHUPB_003634"
     CDS             complement(5387..6316)
                     /gene="pfkB"
                     /locus_tag="ACHUPB_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0760346.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1657 a         1597 c         1564 g         1574 t
ORIGIN      
        1 ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa gtttccttcg
       61 ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga
      121 agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg
      181 tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg tattgataag
      241 ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat cgccgcgatg
      301 gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc agcataagaa
      361 ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt
      421 cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta gcgccgtacg
      481 gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc
      541 catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc
      601 tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt
      661 aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc
      721 gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc
      781 cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt
      841 aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt
      901 aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac
      961 aacgaaactt cccagtttga tgatgtcttg caagttagaa cctgcaacca ctttggtcat
     1021 tagagccaga acgccgtaag gggtcaactg catgacaagg cgaaccagtt tcatcaccca
     1081 gctttgtagg gtatcgatag cggctaagac gcgttcacct ttcggcgcat catccttcag
     1141 cagtttcaat gcagctacgc cgaggaatgc ggcaaaaatt accacgctga taattgacgt
     1201 cggattggct ccggtaagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg
     1261 cggaacgctc agatcagaga ctttaccaac atagttactt tcaatggcgt tcagacgtgc
     1321 agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag
     1381 cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg tcagaaaact
     1441 gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa
     1501 gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa
     1561 ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag
     1621 gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt
     1681 atggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa
     1741 tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag
     1801 tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat
     1861 tccagaaagg tgatttttgc ctttttagta ttcaacctga tgcttaataa gccgatgaaa
     1921 ctggatctgc aaggtattga tcatttgcga tgaccaacga acagcgctgt tctcgggtaa
     1981 tgaatggggc atccataccg accagacaaa taatgccatg cagataaaac gttccagttg
     2041 gttgcccttt tggggaacca ggataggcaa gcggaaacgc cagcggcatg gccagagcag
     2101 gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac ccagcaccat
     2161 tccttgtagc gcatcagccg gaatgaacca gccttccgga accttaaggt aaaaggttgc
     2221 cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc
     2281 gatcggtttt gatatccatt ttaatcgctg cccaagaaac gactttgggt gatcgatgtc
     2341 cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag
     2401 cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg
     2461 accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta
     2521 tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc
     2581 agttgaatgc aaattcagat aaagaaccag gggcagcgtg atcactgccc ctggtcatta
     2641 accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac
     2701 aaaacgtgga tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc
     2761 tttagaagcg atcatgccat ttaccgagtc ttccagcgcc acgcaggtca gtgggtcaac
     2821 gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt
     2881 ttcagccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc
     2941 cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa
     3001 cgcaacagct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc
     3061 acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc
     3121 gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg
     3181 acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat cccgtaaagg
     3241 ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt
     3301 tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt
     3361 aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa
     3421 gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg
     3481 ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc tccacattga
     3541 tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca
     3601 tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc
     3661 tgttctggta taactccccg acccctaatt ttaatggtgg cgtgaacgtt atgttctggg
     3721 tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc
     3781 gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag
     3841 gggaagaggg gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta
     3901 ttttcctgca gtttttctca ctatatatcc tgccggttat ttgtattgct gcaggttatg
     3961 tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg ccattatcct
     4021 caacccatat catgctgcta ataatctatc caatgactgc tgagcaataa ccaaatgctg
     4081 accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc
     4141 aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg
     4201 ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg
     4261 gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg
     4321 caacagagaa ggctgcagtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc
     4381 gtcgatattg ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg
     4441 ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga
     4501 gagcgagtta tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc
     4561 aatttgctga ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc
     4621 catcaccaga aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac
     4681 ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag
     4741 ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc
     4801 atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc
     4861 gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca
     4921 taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta
     4981 ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaagtcg tgatggtgat
     5041 ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc
     5101 ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc
     5161 atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat
     5221 ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact
     5281 ggccatagtt cacccctttg ttatcacatg acttttgagc aaagtcggag aaacgcgttg
     5341 ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt
     5401 aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg
     5461 ctgcactccc cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca
     5521 gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca
     5581 ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac
     5641 caagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc
     5701 ggacatcgtc cggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag
     5761 gcttaaccaa ctcgatgtta ccaattgcca gtgctgcact taacgcttcg ccagaactgt
     5821 cgacgatgca gcggatccct tgtttttgcg aagcggaaat cagttgggtt aatttttcca
     5881 gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca
     5941 gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa
     6001 aacgatactg ctcaccgctt gcttccacat gtacatgtaa attctgccgg gtccagtctt
     6061 tggcttctac agtagcgacg gggacatttt catccgccaa cagtgaaacc aggtgttcgc
     6121 cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc
     6181 gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc
     6241 cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg
     6301 tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcg
     6361 gaaagtgaag aaattaacaa tatgatttat tg
//
LOCUS       DAZOZD010000228         6391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOZD010000228 DAZOZD010000000
VERSION     DAZOZD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.230"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            48..1103
                     /gene="phoE"
                     /locus_tag="ACHUPB_003635"
     CDS             48..1103
                     /gene="phoE"
                     /locus_tag="ACHUPB_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0760347.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1142..1543)
                     /gene="crl"
                     /locus_tag="ACHUPB_003636"
     CDS             complement(1142..1543)
                     /gene="crl"
                     /locus_tag="ACHUPB_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0760348.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1601..2845)
                     /gene="frsA"
                     /locus_tag="ACHUPB_003637"
     CDS             complement(1601..2845)
                     /gene="frsA"
                     /locus_tag="ACHUPB_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0760349.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2937..3395)
                     /gene="gpt"
                     /locus_tag="ACHUPB_003638"
     CDS             complement(2937..3395)
                     /gene="gpt"
                     /locus_tag="ACHUPB_003638"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0760350.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3656..5113
                     /gene="pepD"
                     /locus_tag="ACHUPB_003639"
     CDS             3656..5113
                     /gene="pepD"
                     /locus_tag="ACHUPB_003639"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0760351.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5170..5784)
                     /gene="prfH"
                     /locus_tag="ACHUPB_003640"
     CDS             complement(5170..5784)
                     /gene="prfH"
                     /locus_tag="ACHUPB_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0760352.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5781..6391)
                     /locus_tag="ACHUPB_003641"
                     /pseudo
     CDS             complement(5781..6391)
                     /locus_tag="ACHUPB_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1627 a         1638 c         1575 g         1551 t
ORIGIN      
        1 ggctctcccg ctgaatattc gcgcgttaat taaaatcagg aatgaaaatg aaaaagagca
       61 ctctggcatt agtggtgatg ggcattgtgg catctgcatc tgtacaggct gcagaaatat
      121 ataataaaga cggtaataaa ctggatgtct atggcaaagt taaagctatg cattatatga
      181 gtgataacgc cagtaaagat ggcgaccaga gttatatccg ttttggtttt aaaggtgaaa
      241 cacaaattaa cgatcaactg actggttatg gtcggtggga agcggagttt gccggaaata
      301 aagcggagag tgatactgca cagcagaaaa cgcgtctcgc ttttgccggg ttgaaatata
      361 aagatttggg ttctttcgat tatggtcgta acctgggcgc gttgtatgac gtggaagcct
      421 ggaccgatat gttcccggaa tttggtggcg attcctcggc gcagaccgac aactttatga
      481 ccaaacgcgc cagcggtctg gcgacgtatc ggaacaccga cttcttcggc gttatcgatg
      541 gcctgaactt aaccctgcaa tatcaaggga aaaacgaaaa ccgcgacgtt aaaaagcaaa
      601 acggcgatgg cttcggcacg tcattgacat atgactttgg cggcagcgat ttcgccatta
      661 gtggggccta taccaactca gatcgcacca acgagcagaa cctgcaaagc cgtggcacag
      721 gcaagcgtgc agaagcatgg gcaacaggtc tgaaatacga tgccaataat atttatctgg
      781 caactttcta ttctgaaaca cgcaaaatga cgccaataac tggcggcttt gccaataaga
      841 cacagaactt tgaagcggtc gctcaatacc agtttgactt tggtctgcgt ccatcgctgg
      901 gttatgtctt atcgaaaggg aaagatattg aaggtatcgg tgatgaagat ctggtcaatt
      961 atatcgacgt cggtgctacg tattatttca acaaaaatat gtcagcgttt gttgattata
     1021 aaatcaacca actggatagc gataacaaat tgaatattaa taatgatgat attgtcgcgg
     1081 ttggcatgac ctatcagttt taatggatat tgccggatgt gatacatccg gcacatttca
     1141 ctcacgccgt taacttcacc ggttcatcac gaaaatcatc cgccggttcc agcttcagat
     1201 tcagcgtcgt cagcagttca cgcagcttct cgtgaaactc acgtagggtg tgctccagtc
     1261 gttcaaccac ttcagtgtct tttaccggaa cactcttcca gtcgcctgct ttatcgaaca
     1321 gaccaaactg gtaactgtag gtaaaacggg attcctgcgc ttcaagctcc atccaccagc
     1381 cccagaactc acgcacttcc ggtgccggtt tcacgttgac gcatacagcc agacaatcga
     1441 aaaagaatcg attatcttcg cacttacctt cacgaatata cgggcctagt gcggtaaatt
     1501 ttttgatcaa tctgctcttc gggtgtccac tcggtaacgt cattgcaatc tcctgttgtg
     1561 atgcaactgt tttaccaaat tggcaaattt tagcaaattt ttaacacaag cgtttttcga
     1621 tccagccggt gatttcctga agacctttgt caaaattccg atacaccggg ttaaatggga
     1681 tctctaataa tttaccgtca gcagatgatg aggtgattaa gcgtgagtcc tcttccgggc
     1741 tgaacggatc gttcttccag tagcctgata acattggcgt tgggcagcga cgccccagca
     1801 atccttgcac ttttaatgaa tagcgattca gctccacgcg taacgcttcg tcggaagcat
     1861 catgcatccc caaacgactc gccagaacgt caagatacat ttccggcacc tgttgctggc
     1921 acttaaaatc actcaacagg gtatgaacta ccggaccaag acaggcaacc gctttcagac
     1981 gcggcgattc aagatatgcc agacgcacgg caacgttagc gccgaaacgg aaaccaaagg
     2041 ccgcgacgcg agtgtgatcc acccacggta cgttaggcag agcctttaaa acgtgctgat
     2101 gcaacaggct ggagtcctgg gtgagcttcc attttgatga aaagcccacc gacggcatat
     2161 caatagtcag catcgcaatg ccgcgcggcg caaaataacg ttcatacagg ctgtaatagt
     2221 ccgtctgcat cgcatccaga ccaccacaca ttaatactgt cgggaacggg ccatcgcctt
     2281 tcggcatatg caaaaagccg gtgatgggcg caccgccggg tacggtaaac tccatctgcc
     2341 gcatcgtgcc cggcagacgc tgggcggctt cttcataggc acggtttgac aaagcctgcg
     2401 cttgctcggc caggtcatct cctttcagat gaggataggc agcaatgttg tacaacgtag
     2461 ccgcatgcag ccagtgacga ccactgagtt gcggatcttc ttccgcacag gctttttgtt
     2521 gccacaccat cgcctggatg gcccactcat aaatccagtt gccaccacgg tagccaatca
     2581 ccgtatcgta taaatcatcg tcggtacgtt cggcatcgct catcacaata cgcgcctgga
     2641 cgtcgaggat ttcacgtggg tcaatgccgc gccagatcca catcagacgg ttaatcatgc
     2701 gataccagtg agggatggtt ttaccatcaa gggccgactg cacaggcggt tgtgcgccgt
     2761 gattaaagcg gcggactagc gtcgaggttt caggatgttt aaagcggggt ttgaacaggg
     2821 tttcgctcag gtttgcctgt gtcatggatg cagcctccag aatacttact ggaaactatt
     2881 gtaacccgcc tgaagttaaa aagaacaacg cccggcagtg ccaggcgttg aaaagattag
     2941 cgaccggaga ttggcgggac gaatacgacg cccatatccc acggctgttc aatccaggta
     3001 tcttgcggga tatcaacaac atagtcatca accagcggac gaccagccgg ttttgcgaag
     3061 atggtgacaa agtgcgcttt tggatacatt tcacgaatcg caaccgcagt accaccggta
     3121 tccaccaggt catcaataac gatgaagcct tcgccatcgc cttctgcgcg ttttagcact
     3181 ttaagctcgc gctggttgtc gtgatcgtag ctggaaatac aaacggtatc gacatgacga
     3241 atacccagtt cacgcgccag taacgcaccc ggtaccagac cgccacggct tacggcaata
     3301 atgcctttcc attgttcaga aggcatcagt cggcttgcga gtttacgtgc atggatctgc
     3361 aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa gtgtcccagc ctgtttatct
     3421 acggcttaaa aagtgttcga ggggaaaata ggttgcgcga gattatagag atctggcgca
     3481 ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct ccctgacgcg
     3541 ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgttgac aacattttct
     3601 gctaaccctg tgacctgcaa tactgttttg cgggtgatcg acaaggagac ttaacgtgtc
     3661 tgaactgtct caattatctc cacagccgct gtgggatatt tttgccaaaa tctgttctat
     3721 tcctcacccg tcctatcatg aagagcaact cgctgaatac attgttggtt gggcaaaaga
     3781 gaaaggtttc catgtcgaac gcgatcaggt aggtaatatc ctgattcgta aacctgctac
     3841 cgcaggtatg gaaaatcgta aatcggtcgt cttacaggcc cacctcgata tggtgccgca
     3901 gaaaaataac gacaccgtgc atgacttcac gaaagatcct atccagcctt atattgatgg
     3961 cgaatgggtt aaagcgcgcg gcaccacgct gggtgcagat aacggcattg gtatggcgtc
     4021 tgcgctggcg gttctggctg acgaaaacgt ggttcacggc ccgctggaag tgctgctgac
     4081 catgaccgaa gaagccggta tggacggtgc gttcggctta cagagcaact ggttgcaggc
     4141 tgatattctg attaacaccg actccgaaga agaaggtgaa atctacatgg gttgtgcggg
     4201 gggtatcgac ttcacctcca acctgcattt agatcgtgaa gcggttccag ctggttttga
     4261 aaccttcaag ttaaccttaa aaggtctgaa agggggtcac tccggcgggg aaatccacgt
     4321 tggcctgggt aatgccaaca aactgctggt gcgcttccta gcgggtcatg cagaagaact
     4381 ggatctgcgc cttatcgatt tcaacggcgg cacactgcgt aacgccatcc cgcgtgaagc
     4441 ctttgcgacc attgctgtcg cagctgataa agtcgacgtc ctgaaatctc tggtgaatac
     4501 ctatcaggag atcctgaaaa acgagctggc agagaaagag aaaaatctgg ccttgttgct
     4561 ggactctgta gcgaacgata aagctgccct gattgcgaaa tctcgcgata cctttattcg
     4621 tctgctgaac gccaccccga acggtgtgat ccgcaactca gacgtggcaa aaggtgtggt
     4681 cgaaacctcc ctgaacgtcg gtgtggtgac catgactgat aataacgtag aaattcactg
     4741 tctgatccgt tctctgatcg acagcggtaa agactacgtg gtgagcatgc tggattcgct
     4801 gggtaaactg gctggcgcga aaaccgaagc gaaaggcgca tatcctggct ggcagccgga
     4861 cgctaattct ccggtgatgc atctggtacg tgaaacctat cagcgtctgt tcaacaaaac
     4921 gccgaacatc cagattatcc acgcgggcct ggaatgtggt ctgttcaaaa aaccgtatcc
     4981 ggaaatggac atggtttcta tcgggccaac tatcaccggt ccacactctc cggatgagca
     5041 agttcacatc gaaagcgtag gtcattactg gacactgctg actgaactgc tgaaagaaat
     5101 tccggcgaag taattatttg atttgctgcc ggatggcgtt taatcgcctt ccggcagttt
     5161 catccttcat tatccttcga taaaagccat ccctgtaaat gtccgtctcg ggttgccacg
     5221 ttcaatctgg tgatggaaca ttcgccgctg cgatttcagc accgcgctat tttcctgttg
     5281 ctgctgctcc agcttccagg caatcagcaa tcgtgccagc cgcttgttag catgctgact
     5341 acgctctgac tgaaccttca cgctaatacc ggatgccaaa tgcgtggcgc gtaccgccga
     5401 gtcggtttta ttgacatgtt gaccgcccgg ccccgacgaa cgcaacgtct cataacggat
     5461 tgcatccgat tgttcctgct catcagcggt aaaacgccca atgcccagaa accagttttt
     5521 gcgcccatga tgaggccgat acggactcgg acaaatccac tgaatagtgc cgcaccagct
     5581 ttcgcttaat gcccatgcgt tatcgccatc cagagaaacc agagctgaac gcagcgtgtc
     5641 agagtagcgg cccgtttctg tttccaacac cgttaccgcg acgtcttgtc gggcagcttc
     5701 tttaattaat cgctccagcg cctttttcac tgcgagacaa cattcttccg gcccctgcgc
     5761 tgaggagagt tgtagcaaga tcatcctttt ttccctccac tgtttttgag cgttagcacc
     5821 ggacgcagcc gcgcgacagg aataattaaa ccagcctgca ccagacattg caccacgctt
     5881 tcagccgatt tataggcttg tggcgcttct tcaaagatga gttgtttatc gcgacaaatt
     5941 acccgactgc caagttcagt acgtgagagc tgcgtcgctg tgtatttcgc tgccagacgc
     6001 cctttacact cggtgcgccc ccatttacgc ccagccccat gcgccagcga gtggagcgtt
     6061 ttttcgttcg caacaggctg tacaagctag gagtaatcac cgagtgaacc gggaataatc
     6121 accagaccgt tgtcatccgg cgtggcacct ttacgatgca accagccctg ttgatcgccg
     6181 atttgacacg cgctaacaaa gttatgcgcc acatccagaa ccggacttcc ggtggcctta
     6241 acctgttgca ttatgcgtaa agagatcagc tggcgattaa tacgcgcaaa cgccagcgca
     6301 tcgtcatgtt ccgcaatata ccgtagcgcg tcgtcactgc cttcaggcaa accatgatgc
     6361 gaaaacgagg caatatgccg ctgtaaaata g
//
LOCUS       DAZOZD010000229         6360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOZD010000229 DAZOZD010000000
VERSION     DAZOZD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.232"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHUPB_003642"
     CDS             <1..331
                     /locus_tag="ACHUPB_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0760353.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUPB_003643"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUPB_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0760354.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUPB_003644"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUPB_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0760355.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUPB_003645"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUPB_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0760356.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUPB_003646"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUPB_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0760357.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUPB_003647"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUPB_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0760358.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUPB_003648"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUPB_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR0760359.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1723 a         1506 c         1544 g         1587 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagtgaa aacatcctaa aggagaagtt cagttagccg
//
LOCUS       DAZOZD010000230         6353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOZD010000230 DAZOZD010000000
VERSION     DAZOZD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.233"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPB_003649"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUPB_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0760360.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUPB_003650"
     CDS             2219..2767
                     /locus_tag="ACHUPB_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0760361.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPB_003651"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUPB_003651"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0760362.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUPB_003652"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUPB_003652"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0760363.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUPB_003653"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUPB_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0760364.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1598 a         1558 c         1610 g         1587 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtg
//
LOCUS       DAZOZD010000231         6326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZOZD010000231 DAZOZD010000000
VERSION     DAZOZD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.234"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="ACHUPB_003654"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="ACHUPB_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0760365.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHUPB_003655"
     CDS             complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHUPB_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR0760366.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="ACHUPB_003656"
     CDS             2736..3797
                     /locus_tag="ACHUPB_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR0760367.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHUPB_003657"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHUPB_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR0760368.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..6295
                     /locus_tag="ACHUPB_003658"
     CDS             5678..6295
                     /locus_tag="ACHUPB_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR0760369.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1704 a         1594 c         1513 g         1515 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctggggtat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgctag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 ggggtctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta tttgagtggg
     6301 ataccagagg attatgttat ttatta
//
LOCUS       DAZOZD010000232         6298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZOZD010000232 DAZOZD010000000
VERSION     DAZOZD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.235"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUPB_003659"
     CDS             complement(<1..170)
                     /locus_tag="ACHUPB_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR0760370.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAI"
     gene            251..535
                     /gene="traQ"
                     /locus_tag="ACHUPB_003660"
     CDS             251..535
                     /gene="traQ"
                     /locus_tag="ACHUPB_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR0760371.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            522..1067
                     /gene="trbB"
                     /locus_tag="ACHUPB_003661"
     CDS             522..1067
                     /gene="trbB"
                     /locus_tag="ACHUPB_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR0760372.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            997..1344
                     /gene="trbJ"
                     /locus_tag="ACHUPB_003662"
     CDS             997..1344
                     /gene="trbJ"
                     /locus_tag="ACHUPB_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR0760373.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            1325..1717
                     /gene="trbF"
                     /locus_tag="ACHUPB_003663"
     CDS             1325..1717
                     /gene="trbF"
                     /locus_tag="ACHUPB_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR0760374.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            1704..3077
                     /gene="traH"
                     /locus_tag="ACHUPB_003664"
     CDS             1704..3077
                     /gene="traH"
                     /locus_tag="ACHUPB_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR0760375.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            3074..5899
                     /gene="traG"
                     /locus_tag="ACHUPB_003665"
     CDS             3074..5899
                     /gene="traG"
                     /locus_tag="ACHUPB_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR0760376.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            5896..>6298
                     /gene="traS"
                     /locus_tag="ACHUPB_003666"
     CDS             5896..>6298
                     /gene="traS"
                     /locus_tag="ACHUPB_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628113.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR0760377.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSI"
BASE COUNT         1618 a         1426 c         1658 g         1596 t
ORIGIN      
        1 ataatcgcta taaataacag tatcgtcaca aataaatcag caaaggtttt aatggggagg
       61 gagtgcaata atgaatacac cagtaaagca aaagccgcat tacctgataa tgactctctt
      121 atgatgtttc tcactatttc cagtttttct ttaaaagata actttttcat ttgtacctct
      181 gattaatatc acacaaacgc gaatattaca gacatgaata ctctcatgta aataataacc
      241 ggagtttaac atgataagta aacgcagatt ctctttaccc cggcttgata ttacgggaat
      301 gtgggtattt tccctgggtg tctggtttca tatcgtcgcc cgtcttgttt acagcaaacc
      361 ctggatggct ttttttctgg cggagttgat tgcggccatc cttgtgctgt tcggcgcata
      421 ccaggtgctg gatgcatgga ttgcacgtgt cagcctggaa gagcgtgagg cactggaagc
      481 caggcaacag gccatgatgg aagggcagca ggagggcgga catgtctctc actaaattac
      541 tgccggtcat tttgttgatg atggccacag gtgtgcaggc ctccacccgg gatgaaattg
      601 aacgactctg gaatccgcag ggaatggccg tgcagcctgc acaaccggca gcagacacat
      661 cagccagaac agaaaagccg gctccccgct ggttccgcct cagcaacggc aggcaggtta
      721 acctggccga ctggaaggtg gtgctgttta tgcaggggca ttgcccttac tgtcaccagt
      781 ttgacccggt actgaagcag ctggcacagc agtacggctt ttccgttttt ccctacaccc
      841 tggatggtca gggggatact gcctttccgg aagcattacc ggtgccaccg gacgtgatgc
      901 agaccttctt cccgaatatc ccggtggcca caccgaccac cttcctggtc aacgtcaaca
      961 cacttgaggc attaccgctt ttgcaggggg caacggatgc cgccagtttt atggcgcgga
     1021 tggataccgt tctgcagata tacggagagg aaaaaggtgc gaaataagca ggtggtgtta
     1081 ctcattgccg gaatatccgg catcgcgacg ggaataatcg tcagcctgaa tatccccttt
     1141 atccgtcagg ggctttttta tcccgccagc cctgtggaaa ttgtcgtttc gctgtgtctt
     1201 accttttctg tttctgttgt gttttttgtg ggggcaattg tgggatggat ttcggtgtct
     1261 gaaatatatt acagccgcat gacctgtctg catgaatcat cagaaatatc agagggaact
     1321 tatgatgaaa gaaaaaataa atgatacaga accgggaatc aaacaaattg aaagggagat
     1381 tgaacgtggt tgtgataatg caaaaaaata cttctggctt tttgtggttt tctttgctgc
     1441 cggattgatt gtaaggaacg ttatgcatga ttttttcagt gccggtattg attcgtggaa
     1501 agctgacccg gaattaaata attttcgtta tatgtggaac accctgatgt atgtcattcc
     1561 gattatgtta tatgcgcttg cagctggttt tcttgctgcg gcttctctgt cgccgttgtg
     1621 cgaaataata tttgggggtg tccggatatt cctgctgaaa cgccgtatgc gccgggagaa
     1681 cacattaagg gagggcagca ataatgcttc gcattaaacc tcttctggtt ctttgtgccg
     1741 ctttactgac ggtcacaccc gcagcgtcag cggatgtgaa cagcgatatg aatcagttct
     1801 ttaacaagct gggcttcgcc tccaatacca cacagccggg cgtctggcag gggcaggccg
     1861 ccgggtatgc ctatggtggc tccctgtatg cccgcaccca ggtcaaaaat atccagctga
     1921 tttccatgac gctgccggat atcaatgccg gatgtggcgg catcgacgcc tatctcggct
     1981 cgttcagttt tattaatggc gatcagttgc agcgttttgt taagcagatt atgagcaatg
     2041 ctgccggtta cttttttgac cttgccctgc agacaacggt gccggaaata aaaaccgcaa
     2101 aagacttcct gcagaaaatg gcaagtgaca ttaacagtat gaacctcagt tcctgtcagg
     2161 tggcacaggg gcttgtgggg gggctgttcc ctcagacgca ggaatctcag cagaaagttt
     2221 gtcaggacat tggcagtgaa agcaatatct tcgccgactg ggccgcatct cgtcaggggt
     2281 gcacggtcgg agggcagtcc tcatctgttc gtgacaaagc cagcgacaag gacaaggagc
     2341 gggtgctgaa aaaccagaat atcatatggg aggcgctgaa aaaaaacaga atgtttgacg
     2401 gcaacaaaga gttgaaagaa tttatcatga cgctcaccgg tacgctgatt tttggggaag
     2461 acagcgagat tacccccctg ccggcgcgta ccactgaccg cgatatcctc cgggcgatta
     2521 tggagggggg aacggcaaaa atttaccact gcaacgattc ggacaagtgt ctgaaggtgg
     2581 tggcggatgc aaatgtgacc atcgcccggg ataaagccct gaagtcacag attacaaaac
     2641 tgctcaccag tattcagaat aaagcagtga gtgacactcc gcttgacgcc cgggaacagg
     2701 ggtttatttc cagcaccacc attcccgttt ttaaatatct gattgacccg cagatgctcg
     2761 gtgtgtccaa cagcgtcgtt taccagctga cggactatat cggctacgac atcatgctcc
     2821 agtacattca ggaactgctt cagcaggccc gggccatgat tgccaccggg aactatccgc
     2881 aggctgtgat ggacaatgtt ctggagaatc tgaatcaggc acagcaacag attgccattt
     2941 tccagagtca ggtacaggtc cagcaggatg cgctgctggt tgtcgaacgt cagatgaact
     3001 acatgcgtca gcagctttcc gcccgtatgc tcagccgtta ccagaacaac tatcacttcg
     3061 gagggaattc actgtgaatg aagtttatgt gattgccggt ggtgagtggt tgcggaataa
     3121 cctgaacgcc attgctgcat ttatgagtac ccggacctgg gattcaattg aaaaaatcgc
     3181 gctcaccctg tctgttgttg ctgtggcttt tatgtgggta cagcggcata acgtgatgga
     3241 tttgctgggc tgggtggccg tgtttgtgct tatcagcctg ctggttaatg tccgcacatc
     3301 ggtgcagatt attgataaca gtgacctggt caaagtccat cgggtggata atgttccggt
     3361 cggtctggcg atgccacttt ctcttacgac ccgtatcggg catgcgatgg tggtcagcta
     3421 cgagatgata ttcacccaac cggacagcgt cacctacagc aagacaggca tgctgttcgg
     3481 ggcgaatctg attgtgaaaa gcaccgattt cctgtcccgg aatccggaaa tcatcaatct
     3541 gttccaggac tacgtccaga actgcgtgct gggtgatatt tacctgaatc ataaatacac
     3601 gctggaagat ctgatggcct ccgctgaccc ctacacgctg attttttccc gacccagccc
     3661 gctacggggc gtttatgaca acaataataa tttcataacc tgtaaggatg cgtcggtcac
     3721 gctgaaagac aggctgaatc tcgatacaaa gacgggaggc aagacctggc attattatgt
     3781 gcagcagata tttggcggca gaccggaccc ggacctgtta ttcagacaac tggtcagtga
     3841 cagttacagt tatttctacg gctccagtca gtctgccagc cagattatgc gccagaacgt
     3901 cacaataaat gccctgaaag agggtatcac cagtaatgct gcccgtaatg gtgacaccgc
     3961 cagcctggtg aatctggcca ccacgtcatc gatggagaag caacgtctgg cgcatgtctc
     4021 tatcggtcat gtgacaatgc gaaacctgcc aatggtacag actatcctga cgggaattgc
     4081 cattggtatt ttcccgcttc tggtactggc agctgtcttc aacaaactga cactgtccgt
     4141 cctgaaaggc tatgtgtttg ccctgatgtg gctgcagacc tggccgttgt tgtatgccat
     4201 tctgaacagt gccatgacat tctatgcgaa gatgaatggt gcgccggtcg tgctgtctga
     4261 actctctcag atacagctga aatactctga cctggcctct actgccgggt atctttcagc
     4321 catgatcccc ccgttgtcat ggatgatggt aaaaggcctt ggcgcaggtt tttccagcgt
     4381 gtacagccac ttcgcctctt cctctatcag tccgacggcc agtgcagcag gcagtgtggt
     4441 tgacggtaat tactcctacg gcaacatgca gacggaaaac gtgaacggct tcagctggag
     4501 caccaacagc accacgtcgt ttggtcagat gatgtaccag accggcagtg gcgcaactgc
     4561 cacacagacc cgtgacggta atatggtgat ggatgcaagc ggagcgatgt cccgtttacc
     4621 ggtcggtatc aatgcaacgc gtcagattgc agcggcgcaa caggaaatgg cccgggaggc
     4681 gtcgaacaga gcagaaagtg ccctgcatgg tttcagcagc agtattgcca gtgcctggaa
     4741 cacgctcagt cagtttggtt ctaaccgggg gagcagtgat tctgtcaccg gtggcgctga
     4801 cagcaccatg agtgcacagg actccatgat ggccagccgt atgcgcagtg cagtggaaag
     4861 ctatgcgaag gcgcataata tcagtaatga gcaggcgact caggaactgg catcaacagg
     4921 ttctaagttc tcgtatggcg gatatgcaga tgctcatgct gaatggggga ttaaacctgg
     4981 aattcttggt gttggtggtg gggttggtgt cagaggcggg tttaagggaa gtcttgatcg
     5041 aacggatgat gattcacatt ctgcaagtag tggcagcaga gctacccatg atgctcgcca
     5101 tgatattgac gctaaagcca gtaaggactt taaagaagcc agcgattact ttaccagtcg
     5161 caaggtcagt gagtccggca gccatactga caataatgct gactcccggg tggaccagtt
     5221 gtctgcagcc ctgaactcag cgaaacagag ttacgaccag tacacgacga atatgacccg
     5281 cagccatgaa tatgcggaaa tggcctcccg tactgaaagc atgagcgggc agatgagtga
     5341 agacctgtcg caacagtttg cacagtatgt gatgaaacgc gcaccgcagg atgcagaagc
     5401 tattctgaca aataccagtt cgccggagat tgcagaacgt cgtcgggcga tggcctggtt
     5461 ttttgtgcag gaacaggtac agcctggtgt tgataatgcc tggcgggaat cccgtggcga
     5521 tattggtaaa ggaatggaga gcgtaccttc gggtggtggc agtcaggata ttattgctga
     5581 ccatcaggga catcaggcca ttattgagca aagaacgcag gacagtaata tccgtaatga
     5641 tgtaaaacat caggttgata atatggtcac agaatatcgt ggcaatatcg gagatactca
     5701 gaacagtatc cgtggcgagg aaaatattgt aagagggcag tattctgaac tgcaaaatca
     5761 tcataaaaca gaggcattat ctcagaataa taaatataat gaagagcgag aaagtcaaaa
     5821 gataatacct gagccatcag aagaggctgt gaaaaaaatg gttgatgata aaaaggatag
     5881 actgaagggg ccattatgat cactcactat gatataaaaa cggagacaca gaaactaaag
     5941 gatgtactct ctgtagaggg agttaatatt ccgccattat tacaagtcat aaaaccaggg
     6001 gtgtatgtgt ttttatgggt tttattatgg ccgacatttc ttcgtttgct ggcagataaa
     6061 gttgatatcc gagatgctgg atttgatata tgttttagtg gcgtaatggg ttttatcctt
     6121 tttgttgcca ttactaatgg aatgatgttg tatcttgcca tccctaaaaa attcagagat
     6181 gagtcgaaag ttatatcttt tatgtatgat aagaacaaaa cttatatctt gtcgtttgtg
     6241 attgtttttt caatagtttc ctttgctcat acgttcttat ttgggttttt atcaataa
//
LOCUS       DAZOZD010000233         6182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOZD010000233 DAZOZD010000000
VERSION     DAZOZD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.236"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            8..1468
                     /locus_tag="ACHUPB_003667"
     CDS             8..1468
                     /locus_tag="ACHUPB_003667"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0760378.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1504..1707)
                     /gene="ydfZ"
                     /locus_tag="ACHUPB_003668"
     CDS             complement(1504..1707)
                     /gene="ydfZ"
                     /locus_tag="ACHUPB_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0760379.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1884..2570)
                     /gene="rspR"
                     /locus_tag="ACHUPB_003669"
                     /gene_synonym="ydfH"
     CDS             complement(1884..2570)
                     /gene="rspR"
                     /locus_tag="ACHUPB_003669"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0760380.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2659..3405)
                     /gene="ydfG"
                     /locus_tag="ACHUPB_003670"
     CDS             complement(2659..3405)
                     /gene="ydfG"
                     /locus_tag="ACHUPB_003670"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0760381.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3542..5587
                     /gene="dcp"
                     /locus_tag="ACHUPB_003671"
     CDS             3542..5587
                     /gene="dcp"
                     /locus_tag="ACHUPB_003671"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0760382.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5632..6150)
                     /locus_tag="ACHUPB_003672"
     CDS             complement(5632..6150)
                     /locus_tag="ACHUPB_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0760383.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1536 a         1480 c         1595 g         1571 t
ORIGIN      
        1 aattgttatg ggaaataatt tgttatcagc aaaagcgaca ctccctgttt atgatcgtaa
       61 taacctggcc ccaagaatta ttcatttagg ctttggtgca tttcaccgtg cgcatcaggg
      121 cgtgtatgcc gatattcttg ctacagaaca tttcagtgac tggggatatt atgaggtcaa
      181 cttaatcggc ggcgaacagc aaattgccga tttacaacag caagataatc tttataccgt
      241 tgcggaaatg tcggccgatg cgtggacggc tcgcgtcgtt ggcgtcgtta agaaagcctt
      301 gcacgtacag atggatggct tagaaaccgt gttggctgcg atgtgtgaac cgcaaatcgc
      361 gattgtctct ctgacaatca ccgaaaaagg gtatttccac tctccggcga ccggacagtt
      421 aatgctcgat cacccgatgg tcgctgccga cgtgcaaaat ccccaccagc cgaaaacagc
      481 aacaggggtg attgttgagg cgctggcccg ccgtaaagcg gcaggacttc ccgcatttac
      541 cgtcatgtca tgtgacaaca tgccagaaaa cggtcatgtt atgcgtgacg ttgtcacttc
      601 atacgcgaaa gctgttgatg taaaactggc acaatggatc gaagataacg tgactttccc
      661 atcaacaatg gtggaccgta ttgtgcccgc agtgacagag gatacgctgg cgaaaatcga
      721 acaacttacc ggtgtgggcg atcctgcggg cgttgcctgt gaacctttcc gccagtgggt
      781 aatagaagat aactttgttg ccggacgtcc agaatgggaa aaagcgggag ccgaactggt
      841 tagtgatgtg ctgccttatg aagagatgaa gttgcgcatg ctcaacggca gtcattcatt
      901 cctggcgtat ttgggttatc ttgctggata tcagcacatt aatgactgca tggaagatga
      961 acattatcgt tatgcggcgt atggcttgat gttgcaggaa caagcgccga cgctgaaagt
     1021 gcagggcgtt gatttgcaag attacgctaa ccgattaatt gaacgctata gcaacccggc
     1081 gttacgtcat cgaacctggc agattgcgat ggatggcagc cagaaattgc cacagcggat
     1141 gttggattct gttcgctggc atctggcgca tgacagcaag ttcgatctgc tggcactggg
     1201 cgtcgcgggt tggatgcgtt atgtcggtgg tgttgatgaa cagggaaatc cgattgaaat
     1261 cagtgaccca ctgttacctg ttattcagaa ggctgtacaa agtagtgctg aagggaaagc
     1321 gcgcgtccag tcattgctgg cgattaaggc gatctttggt gatgatttgc cagacaatag
     1381 tttgtttact gcaaaagtga cggaagcgta cttgtcttta ttagcgcatg gcgcgaaagc
     1441 gaccgtggcg aaatattccg tgaagtaaaa aaagcaatgc cgccatcggc ggcattcaca
     1501 cgcttagttc atgccgagac gaatcaggtc cagaggtgcc agtttctctt cacaaccttc
     1561 aacaactacg gtttttccgc ggcggatttg ctcagcggtc agaccttcag tatcaatcga
     1621 caggatcttg ccagtgtgac cggtgccgct aaccataaca cggctgccag tggtgattgc
     1681 gttacggtta cgatcgtagg tcgtcatagg tattttctcc tttctgattt acagtcgaag
     1741 cccacgtaac gggcgcaata taaatactcc tgtcgagtga aatatttttt gtttttgatc
     1801 aagctcacat cttttttgat acatgcatga gccataaggc gggggaaaag actctcaccc
     1861 cggatgggat gagaggaaat gaattactct tcgctaaacc agtcgctatt ttcctggcgg
     1921 atctggcgca cggattcgct gatttcctgc aaatgttgcg tcattgcacg ttctaccgca
     1981 tcgccatcac gtttttgcag ggcagagaaa atatcaagat gctggcgtaa cagcatttct
     2041 ggtggagaaa cgtggtcgaa actcatatag cgcacgcgat caacggtcgc tttcaggttc
     2101 tcaatggtat cccacgccag ttgacagtcg gcaatctgcg tcaggagttg atggaagttg
     2161 tcatcaagtt caaaaaaatc atccagttgc ttgcgctcaa tggcaatgcg ttgctggtga
     2221 agattttgtt ccagttggta gcactggctt tcggtaatca tgctcgccgc ccgacgcgcc
     2281 accgcgcact caatggcctg acggataaaa ctgccattgc gcacctgggc catggaaatt
     2341 ttgttgacgt agctgccacg ctgcggacga atttgaatca ggccgttttc cgccagttta
     2401 ataaaggctt cacgaaccgg ctggcgtgac acattgaaac gaacagaaac ttctttttcc
     2461 gacaacggtg tgcctggtgc aatcaggcaa tggacaatgt cgcgacgaag aatacgataa
     2521 atttgctgat tgacaggctg tgtgggatta agttgcgttt cgacggtcat tcgttcttgc
     2581 ttgtgagtga gttaactgca tgagtctacc acttttttgt gacaagcaat aacccggcag
     2641 ttacgccggg tataaaaatt actgacggtg gacattcagt ccggcatagc tttgggtaac
     2701 cggcatcatt tccagggtat tgatattgac gtgagcaggc agcgttgaca cccaccagac
     2761 ggcttcgctg acatcttctg gcgtcaatgc aacggtattt tgataggttt tttccgcttt
     2821 accgtcatcg cctttaaagc ggacattgga aaactcggtg ccacccacca gacccggttc
     2881 gatgtcggtg acgcgcaccg ccgtaccatg caggtccgta cgcagattca ggctaaactg
     2941 acgaacaaac gctttcgtcg caccgtaaac gttaccaccg gcatacggcc agctacctgc
     3001 cgttgagcca atgttaataa tatgaccatg attacgttca accatacccg gtaagacggc
     3061 gcgcgtcata tataccaggc ctttgttgtt ggtatcaatc atcgtttccc agtcttcaac
     3121 gctggcttta tgcgcaggct ccatgcccaa cgccaggccg gcattattta ccaggatatc
     3181 aatattgcac cactcggcag gaagcgatgc cagcatctct tcaatagcgg cgcggttgcg
     3241 aacgtccagt tgggcgatat acagattatc tcccagttcg tcttttaact cctgcaaccg
     3301 ctcctggcga cggccagtgg cgataacttt atgcccttgt tgaataaaac gacgagtaat
     3361 gcattcacca aaacctgccg ttgctccagt tactaaaacg atcatctcac tgttcctcaa
     3421 cgcttttgtg tgtcaataac atagcacgtg atgcttaaga gcgggtaata ctggtttgca
     3481 agattgcact ccgttatcag taagcctacg ctatggaaca tctcgcgttc aggagaagaa
     3541 aatgacaaca atgaatcctt tccttgtgca aagcacacta ccgtatctgg ctccccattt
     3601 tgatcaaatt gccaatcatc actatcgccc ggcattcgat gagggaatgc agcaaaagcg
     3661 ggcagaaatt gctgccatcg cgcttaaccc gcaaacgcct gatttcaaca atactattct
     3721 ggcactggaa caaagcggag aattacttac ccgcgttacc agcgtctttt ttgcgatgac
     3781 tgcggcgcat accaatgatg aattacagcg tcttgatgaa cagttttccg ctgaactggc
     3841 ggaactggct aatgatatct atctgaacgg tgaattattc gcgcgggtag atgctgtctg
     3901 gcagcgccgt gaatccctgg ggcttgatag tgaatccatc cgcctggtgg aggtgattca
     3961 tcaacgtttt gtccttgccg gagccaaact tgcgcaagct gataaagcaa aattaaaagt
     4021 actgaataca gaagctgcga ccctgaccag ccagtttaac cagcgattac tggcagcaaa
     4081 taaatccggc ggtctggttg tgaacgatat cgcgcagctg gcaggaatga gtgagcaaga
     4141 gattgcgctg gcggcagagg cggctcgcga gaaaggtctg gataacaaat ggctgattcc
     4201 gctgctgaat accacccaac aaccggcgct tgccgaaatg cgcgatcgtg cgacgcgtga
     4261 aaaactgttt attgcgggct ggacgcgagc ggaaaaaaat gatggcaatg atacccgcgc
     4321 tatcattcaa cgtctggtag agattcgcgc acagcaggcg aaactgcttg gttttcctca
     4381 ttatgccgca tggaaaatcg ccgatcagat ggcaaaaacg ccagaagcag cactcaactt
     4441 tatgcgggaa attgttccag cggcgcgtca acgtgcgagc gatgaattag cctccataca
     4501 ggcggttatc gataagcagc agggcgggtt tagcgcgcag ccgtgggact gggcatttta
     4561 tgcggaacag gtacggcggg agaaatttga tcttgatgaa gcgcagctca agccatattt
     4621 tgaattaaac acggtgttga atgaaggtgt attctggacc gcgaatcagc tcttcggtat
     4681 taagtttgtc gaacgttttg atattcctgt ctaccatcct gacgttcggg tgtgggaaat
     4741 ttttgatcat aatggcgtgg ggctggcgtt attttacggt gatttcttcg cccgtgattc
     4801 aaaaagcggc ggtgcatgga tgggcaattt tgttgagcaa tcaacgctta atgaaacgca
     4861 tccggtaatt tataacgtct gcaattatca gaaacccgct gccggtgagc ctgcgttgtt
     4921 actctgggat gatgtaataa ccttattcca tgaatttggt catacgctgc acggcctttt
     4981 tgcccgccag cgttatgcca cgctttccgg caccaacacg ccgcgtgatt ttgtcgaatt
     5041 tccgtcgcaa atcaacgaac actgggcaac gcatccgcag gtattcgctc gctacgcccg
     5101 gcattatcag agcggggcag caatgcctga cgaactgcaa cagaaaatgc gtaatgccag
     5161 cctgttcaac aaagggtatg agatgagcga actgcttagc gccgcacttc tcgatatgcg
     5221 ctggcattgc ctggaagaaa acgaagcaat gcaggatgtc gatgattttg aattgcgggc
     5281 gctggtggcg gaaaatatgg atcttcctgc tataccgcca cgctatcgca gcagttattt
     5341 cgcccatatt tttggtggcg gatatgctgc aggttattac gcttatctgt ggacgcaaat
     5401 gttggccgat gatggttacc agtggtttgt tgagcagggc ggattaacgc gtgaaaatgg
     5461 gcagcgtttt cgcgaggcga tcctttccag aggtaacagc gaagatctgg aacgcctgca
     5521 tcgacaatgg cgcggtaagg ctcctcagat tatgccgatg ctgcaacatc gtggcttgaa
     5581 catataaaca ccaccaatgg ctggcctggg gatatcgcca ggccagcctg gttatagcag
     5641 taattgcagc agctgggcga gggcaaacct caccgctaaa gctaataccg tttcgcagtc
     5701 gccagcaaaa tgcataaccg cagtgtagtt ctggccttta atatgccacg cgaaccagac
     5761 ggtacccgct ggcgtaccat cttcaccgcc ctccggtccg ccgtagccgg taatggcaat
     5821 actgacatca gcatccgcac gctctatggc acctgttgcc atttctgctg ccactttctc
     5881 actcaccgca gaatatcgtt caagagactg ctggcttacg ctgaggattt tcatctttgc
     5941 ctgatcggtg aaagtaacaa agcctgcacc gtaaaatttg ggtgtatctt cagctgcaca
     6001 cagggcgcta gccaacttac cgccggtgca tgattcggcg gtagttaagc gtaattgatc
     6061 cgcgataagt ctttgactca acgcggatgt caggttttca atagtgtcgg tatctgcgag
     6121 ttgtacaatt ttatctttat taatgttcac tgttttttgt ccacgctatt atatgtaaag
     6181 gt
//
LOCUS       DAZOZD010000234         6131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOZD010000234 DAZOZD010000000
VERSION     DAZOZD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.237"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            3..827
                     /locus_tag="ACHUPB_003673"
     CDS             3..827
                     /locus_tag="ACHUPB_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR0760384.1"
                     /translation="MPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIYLP
                     PLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKITS
                     ALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYAGT
                     TIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGNDP
                     IIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(968..1741)
                     /gene="uxuR"
                     /locus_tag="ACHUPB_003674"
     CDS             complement(968..1741)
                     /gene="uxuR"
                     /locus_tag="ACHUPB_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0760385.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1956..3416)
                     /locus_tag="ACHUPB_003675"
     CDS             complement(1956..3416)
                     /locus_tag="ACHUPB_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0760386.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3497..4681)
                     /gene="uxuA"
                     /locus_tag="ACHUPB_003676"
     CDS             complement(3497..4681)
                     /gene="uxuA"
                     /locus_tag="ACHUPB_003676"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0760387.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5021..>6131
                     /gene="gntP"
                     /locus_tag="ACHUPB_003677"
     CDS             5021..>6131
                     /gene="gntP"
                     /locus_tag="ACHUPB_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0760388.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1436 a         1600 c         1573 g         1522 t
ORIGIN      
        1 ctatgccatt aagcaatgcc cttcagagtc aaattattac agataatcac ttccttcatc
       61 acccaatagt cgacagtgag tttacacata aattaaaata tgcacgcatg gatttggaaa
      121 acatctatct tccgcctctt actcgtggca ataaccataa ttacgatgga aaaagcgctg
      181 tcgaaattag aaaacttgat attagtaaag agtcctggcc atttaattat gtgacaggag
      241 catgcagaga atccgatggt attacaacaa caggacgtat gctctatcgg aacttgaaaa
      301 tcacctcagc tttagacgaa atatatggtg gcatatgtaa aaaggcgcat gctgccacag
      361 agttagcaga aggtttacgg ctaaatttat ttatgaagag ccctttcgat cctgttgagg
      421 actatacagt tcatgaaata acattaggcc ctggttgcaa tgttcctggt tatgccggaa
      481 ccacgatagg atatatttca acccttccag cttctcaggc aaaaagatgg accaatgagc
      541 agccaaggat tgatatctat attgatcaga taatgacggt aacaggggta gcaaattctt
      601 ccggattcgc ccttgccgca ttacttaatg ctaatattga gttgggtaat gacccaataa
      661 ttggtataga agcctatccc ggtaccgcgg agatccatgc aaaaatggga tataaagtta
      721 tcccgggtga tgaaaatgca ccactcaaac gtatgactct gcaaccgtca tctcttccag
      781 aactgtttga attaaaaaat ggtgagtgga attatattgg taaataacta ccaggaagaa
      841 tgagtactaa cattcaggtt atacagatag ctaaacaata ccctgcaatt tacaaagtaa
      901 tactcattgc gacttcttaa tttaagaaag cgcaaggaac atttaccctt gcgcttatta
      961 taataagtca ggcgtcaact ttatccagcg gccatgaatc aaacagatag ccatcaaaat
     1021 aaatatcgtc aacgttcgag aattccagca gacgttgctt aacgttttcc agatgccgcc
     1081 acattgccag cttcgccgct cgggcatctt ttttgattaa cgcggcgagg atctgtttgt
     1141 gatcgcccaa ccactctttg cgatacaggc tgtcatccag atggctgtgc aactggatcc
     1201 acattggatt gttttcccgc cactgccagg actgacggaa cagctccacc agcatgctgt
     1261 tatgcgttgc ttcggcaatg gcgagatgga actgcatatc accactttcg ctgctgcccg
     1321 gcgcgctgga agccagctca cgctcttcca gttgcaatgc ctgacgcatt ttgacgatat
     1381 cttcgcgggt agcctgcaaa gcggcaaatt ccgcgatgtt actttccagt aactgacgcg
     1441 cctgtaacag ctcaaaagga ccggcatcat tgcagacgtt agcatccgga ctgtctgtgt
     1501 tgtggctgcc tgagctatca agaacataga taccggcacc ccggcgcact tccaccagcc
     1561 ctttgatctc cagcatgatc agcgcttcac gtaccaccgt cctcgtgaca tcaagcattt
     1621 ctgcaatttc acgctccggc ggcagccgtt cgccaggatt gtatggcgtc tttatgatca
     1681 gatcgcggat catcgccccg acttcctggt aaggtctttg cgcagaggtg gcagatttca
     1741 tggtgttgtg tccgttaggt gcggttaatc aaatttttgc cggatgcggc gtgaacgcct
     1801 tatccggcct acaacgggca attttacggc acccgttaat ctcaaactat accattagca
     1861 ctggaagttg gcacgtacag gtgggcaaaa aaacgaaaac tggtcaacca aatatcagtt
     1921 tgattgacca gtaagtctgt agggtaatca gttagttaca gcgcagccac acactcgcgc
     1981 gcaccgcgtt cgcacagctg ctgatatgcc gccgtcaccg cgccaacaaa gtcggcattc
     2041 tgcggcagat catcggcaaa aatgccgctc aggcccagca gcgctttcac gcggtctgcg
     2101 ccctgatact gcgcgttaat cttctggaac tcggccagca tcgggtcaac cacgtcaatg
     2161 gcattaccct gttcatccac gccctgggtg tagcgcatcc agccagccac gcccagtgcc
     2221 aggtgacgcc agctgccgcc gttttgcagg tgcagacgca ccggatccag aagacgctgc
     2281 ggtaacttct ggctgccgtc cattgcaatc tgccaggtac ggtgacgcag agacgggttg
     2341 ctgaaacgct cgatcagcag cgtcgcatag gcgttcaggt ctgtaccttc cggcatcgac
     2401 agcgttggcg cttgttcctg catcatcagg gcaaaggccg ctttgcgata atccggatta
     2461 gtcatggtgt cggcaatggt ttcatagccg ccgaggtaac cgaggtacgc caggaaagag
     2521 tggctgccgt tcagcatacg cagtttcatc atttcgaacg gcacaacgtc tgcaacgaac
     2581 tgtgcgccca ctttatccca gtccggacga ccattaacga agttatcttc aatcacccac
     2641 tgacggaacg gttcgcaggc aatggcgcac gggtcgtaaa cacccagctg gtcagcaatt
     2701 tcctgtaagg tttctggcgt cgccgccgga acgatgcggt caaccatggt gcacgggaag
     2761 gtgacgttct cttcaatcca tgccgccagc tgcggatcac gtgcctgagc cagccccaga
     2821 accgcgacct tcgccacatg accgttttca cgcacgttgt cgcaggacat caccgtaaac
     2881 gctttcagcc ctttttcacg acgcagacgc aaggcttcga cgatgtaacc aatcgcggac
     2941 ttcggcgcag tcgggttttc cagatcgtgc ttgatcagcg ggttattgag atccagctga
     3001 ccgcttgccg catcggcgca ataacctttc tctgtgaccg tcagggagac aatcgccgtc
     3061 tgcggacgcg ccatcgcgtt gagaatacct tcacaaccgt cgatttccgg atgcagggct
     3121 tctttcatcg aaccgataat tttcagctcg gtgctttctg cgcctttttc cgccacggtg
     3181 tacagcagtt gctgtttttt caggttttca atcagcacac ggtcgttgcc tggcatcagg
     3241 ttaacttcgc agatacccca gtcgctgtcg gtactttcca gcagatgatg ggtgtacagc
     3301 gcctggtgcg cgcggtgaaa cgccccgcaa ccgagatgca caatgcgtga ttccagacga
     3361 gaatgatccc atgacgggcg ggcaaccggc agattgctgt caacaatagt agtcatcgta
     3421 aactccagaa tccgtcttca gagagtgacg gcaaggaaga aggaaccggc acggcgcgca
     3481 gccatgccgg tgggtatcaa cggctaaaga aagcgcgctg gatcgccagt tcgacaccgc
     3541 gaacttcagc caggcctttc agacgaccaa ttgcggagta acctgggttg gttttcttct
     3601 tcaggtcgtc cagcatctga tgaccgtggt ccggacgcat cgggatcagg tcttctttgc
     3661 cttccgcttt acgacggtgt tcttcttcaa caatcgcttt caccacttcg tacatatcaa
     3721 cgtcaccgtt caggtgcgcc gcttcgtgga aggttttcgg gttatcttca cgcatggtgg
     3781 agcgcagatg ggtgaagtaa atacgcggac cgaactgctt gatcatatca accagatcgt
     3841 tgtcagcacg cacgccgtag gaacctgtgc acatggtgaa accgttcgcc atgctgttta
     3901 cggtatcaac catccactgc atatcttcaa tggtggaaac aatgcgcggc aggccgagga
     3961 tcgggcgcgg cggatcgtcc gggtgaacag ccatacggac gccaacttct tcggcaaccg
     4021 gaataatcgc tttcaggaag acggcaaagt tttcgcgcag tttggctttg tcgatatctt
     4081 tatacagctc caggtgctta cggaactggt cgagggtata cccttcttcc gcgcccggca
     4141 gaccggcaat gatgttacgg gtcagacgcg ctttgtcttc gtcgctcata gtggcgaagc
     4201 gttcagcggc ctgagcaatt tcttcttcgg tgtaatccgc ttccgcgcct ggacgcttca
     4261 ggatatgcat ttcgaatgca gcgaattcga tctggtcgaa gcgcagagct ttggagccgt
     4321 ctggcagcac gtattcgagg tcagtacggg tccagtcgag caccggcatg aagttgtagc
     4381 acacggtgcg aatgccgcac tgcaccaggt tgcgcagagt ctgctgatag ttagcgatcc
     4441 actgttcata gttgccagtg tgggttttga tatcttcgtg aattggcacg ctttctacga
     4501 cagaccatac caggcctgcg tcttcaacga tcgccttgcg tttgaggatc tcttctacgg
     4561 accatacttc gccgttcggg atatggtgca gcgcggtaac cacgccagtt gcgcccgcct
     4621 gacggacatc agctagagaa accggatcgt ttgggccgta ccagcgccag gtctgttcca
     4681 tgatttttcc tctttcacat agtgggttcc acagcaaaga gacgtggagc cccggagttg
     4741 tttaggtatc cggatgaaca accagaccgc agccgtggcg ttgcatcgga tgagatggcg
     4801 tataagttct accttttggt aagacatgtc aacccgaaat ctgaaattgg ttaaccacat
     4861 cgcaagaatt tcactctcac ggttgttgct gcaaattgca gcaaagggta atcgatggtt
     4921 attgatcaga ggatgtgaca ttcatcgcaa caatggttga ccaatttaca taacatgtcg
     4981 cccaaaataa caacggttca accaggtaag gtaaaacgtt atgcatgtgc ttaacattct
     5041 ctgggtggta ttcggcattg gcctgatgct ggtactgaat ttgaagttca aaatcaattc
     5101 aatggtggct ttgctagtgg cggcgctgtc cgtcgggatg ctggcgggca tggatttgat
     5161 gtcgctgctg cacacaatga aagcgggctt cggcaacacg ctgggggaac tggctatcat
     5221 cgtggtgttc ggtgcggtca tcggtaaatt gatggtcgac tccggcgcgg cgcaccagat
     5281 agcgcatacg ctgctggcgc gtctcggtct gcgctatgta cagctgtcgg taattatcat
     5341 cggcctgatt ttcggtctgg cgatgttcta tgaagtggcc tttatcatgt tagcgccgct
     5401 ggttattgtc attgccgccg aagctaaaat cccgtttctg aaactggcga tcccggcagt
     5461 agcagctgcc actaccgcac attcactgtt cccaccgcag ccgggtccgg tggcgctggt
     5521 gaatgcttat ggcgcggata tggggatggt ttatatctat ggcgtactgg tgacgatccc
     5581 aagtgtcatc tgcgcaggtc tgatcctgcc gaagttcctc ggcaatcttg agcgcccaac
     5641 gccatcattc ctgaaagcag atcaaccggt agatatgaat aatctgccct ctttcggcgt
     5701 ttcgattctg gtgccgctga tcccggcgat cattatgatc tctaccacca tcgccaatat
     5761 ctggctggta aaagataccc ctgcctggga agtggttaac tttatcggtt cctcgccgat
     5821 tgcaatgttt attgcgatgg tggttgcatt cgtactcttc ggcaccgcgc gcgggcacga
     5881 tatgcagtgg gtgatgaacg cttttgaaag cgcggtgaag agcattgcaa tggtgattct
     5941 gatcatcggt gcgggtggcg tgctgaagca gaccatcatc gacaccggca ttggtgacac
     6001 catcggcatg ttaatgtccc acggcaatat ctcgccctac atcatggcat ggctgatcac
     6061 cgtactgatt cgtctggcga cgggtcaggg tgtggtttca gcgatgaccg ccgccgggat
     6121 tatcagtgct g
//
LOCUS       DAZOZD010000235         6128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOZD010000235 DAZOZD010000000
VERSION     DAZOZD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.238"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            72..848
                     /gene="exuR"
                     /locus_tag="ACHUPB_003678"
     CDS             72..848
                     /gene="exuR"
                     /locus_tag="ACHUPB_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0760389.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1088..1171
                     /gene="yqiM"
                     /locus_tag="ACHUPB_003679"
     CDS             1088..1171
                     /gene="yqiM"
                     /locus_tag="ACHUPB_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0760390.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1193..1855
                     /gene="yqjA"
                     /locus_tag="ACHUPB_003680"
     CDS             1193..1855
                     /gene="yqjA"
                     /locus_tag="ACHUPB_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0760391.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1859..2242
                     /gene="mzrA"
                     /locus_tag="ACHUPB_003681"
     CDS             1859..2242
                     /gene="mzrA"
                     /locus_tag="ACHUPB_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0760392.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2389..2757
                     /gene="yqjC"
                     /locus_tag="ACHUPB_003682"
     CDS             2389..2757
                     /gene="yqjC"
                     /locus_tag="ACHUPB_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR0760393.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2795..3100
                     /locus_tag="ACHUPB_003683"
     CDS             2795..3100
                     /locus_tag="ACHUPB_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0760394.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="ACHUPB_003684"
     CDS             3103..3507
                     /locus_tag="ACHUPB_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0760395.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="ACHUPB_003685"
     CDS             3497..3796
                     /locus_tag="ACHUPB_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0760396.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4374
                     /locus_tag="ACHUPB_003686"
     CDS             3982..4374
                     /locus_tag="ACHUPB_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0760397.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4444..5429
                     /gene="yqjG"
                     /locus_tag="ACHUPB_003687"
                     /pseudo
     CDS             4444..5429
                     /gene="yqjG"
                     /locus_tag="ACHUPB_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5722..6087
                     /locus_tag="ACHUPB_003688"
     CDS             5722..6087
                     /locus_tag="ACHUPB_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0760398.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1533 a         1513 c         1544 g         1538 t
ORIGIN      
        1 tatgaccaat ggcataccca tgaataaagt ggtatgataa cttcgtccgc attccactcc
       61 gggagacgca tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc
      121 tgaaagagcg cattgaacag ggcgtctatc tggtgggtga taaactgcct gccgaacgct
      181 ttattgccga tgaaaagaac gtcagccgaa cggtagttcg tgaagccatc atcatgctgg
      241 aagttgaagg ctatgtggag gtgcgtaaag gttcaggtat tcatgtggtt tccaaccagc
      301 cgcgccatca gcaggcggct gacaataata tggaattcgc caattacggt ccgtttgagt
      361 tgcttcaggc tcgccagctc atcgaaagta atattgccga gttcgcggca actcaggtaa
      421 cgaaacaaga catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc gaacaatgct
      481 tccgtgattc cgagtgggat ttacagttcc acattcaggt agccctggcg acgcagaact
      541 ccgccctggc ggccatcgtt gaaaaaatgt ggacccagcg tagccataac ccgtactgga
      601 aaaaactaca cgaacacatt gattcccgta ccgttgataa ctggtgtgat gaccacgatc
      661 aaattctcaa ggcgttaatt cgcaaagatc cccatgctgc taagctggca atgtggcagc
      721 atctggaaaa caccaaaatc atgttattta acgaaaccag cgacgatttc gagttcaatg
      781 ccgaccgcta tctattcgcc gaaaacccgg tagtgcatct cgatacggcc accagtggca
      841 gtaaatgaaa ctcctgtccg acaggcgctg cgacgcgcct gttcgcttcg tcggtaagcg
      901 aaaagtataa agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc attcagcaaa
      961 atcagactcc acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag
     1021 attcaatttg aatttatgtt tttgaatgct ttcttatctc acgatttaac agggaatagt
     1081 tcaggctgtg ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt
     1141 agtgcatctt ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact
     1201 tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc
     1261 gatgattggc atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa acggcttgct
     1321 tccagcggcc tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa
     1381 aggcgcgatg ggttacccgc aaacgattct gctgctgacc gttgccgcca gcctcggctg
     1441 ctgggtcagc tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct
     1501 atctcattta cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt
     1561 atcggcgctg ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc
     1621 cgggttatca gggctgaata acgcgcgctt tcagtttttc aactggatga gcggtctgct
     1681 gtgggtattg atcctgacaa ctctgggtta catgctcggc aaaacgccga tatttttaaa
     1741 gtacgaggac cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg
     1801 cctggcaggt tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat
     1861 gcaaatacct cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt tacttctggt
     1921 cggcacgctg ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg
     1981 tgccgttcat caaggcacaa cgatgccaga cggtttttcg atctggcatc accttgacgc
     2041 tcatggcatt cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacatttga
     2101 ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta
     2161 catcattgcg cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttacggga
     2221 taactctcat cgcttcggat aacttcctgt tatccgaaac atatcactca ctttggtgat
     2281 ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac actaccgctt cgttgtctgg
     2341 acaatcgttc ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc
     2401 atcgctttag ctgtttctct ctttgctctt agtgccggta gttatgccac taccctgtgt
     2461 caggaaaagg agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag
     2521 aatcgtattg acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc
     2581 cagctgcgtg ccgatcatca caagaaaatc gcaaagcaga aagatgaggt ggcggaacgc
     2641 cagcaagatt tagccgaggc gaagcaaaaa ggcgatgccg ataagattgc caaacgcgaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtagc gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt
     3841 ttgaagattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taataccgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgc
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgccgct ggtgattctg
     4141 cttgagttcg gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cacagccctg
     4201 tttactgcgg gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc
     4261 gtcaactcgc tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca
     4321 attaccggtc cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc
     4381 gctatactta acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga
     4441 aaaatgggtc aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc
     4501 ggcggtaaat ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgca
     4561 cctggcccca ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatatt
     4621 tcactcgcct gcccgtgggc gcaccgcacg ctgatcatgc gcaaactcaa aggactggaa
     4681 ccgtttattt ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat
     4741 gacagttttc cgggagcaac cggagatacg ctctatcaac atgaatttct gtatcagctt
     4801 tatctccacg ccgatccaca ctacagcgga cgagttactg ttcccgtact gtgggacaaa
     4861 aagaaccaca ccatcgtcag caacgaatca acagaaatca tccgcatgtt caataccgcg
     4921 tttgatgcgc tgggcgcgaa agcgggtgat tactacccac cagccctgca aacgaaaatt
     4981 gacgaactta acggctggat ttatgacacc gttaacaacg gcgtgtataa agctggtttt
     5041 gccaccagcc agcaagccta cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga
     5101 ctggagcaga ttttaggcca gcatcgttac ctgaccggca accagctaac cgaagccgat
     5161 attcgcctgt ggactacgct ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt
     5221 gataagcacc gcatcagcga ttacctgaat ctgtatggct tcctgcgcga tatctaccag
     5281 atgccgggga tcgccgaaac tgtcaatttc gatcacatcc gtaatcatta cttccgcagc
     5341 cataagacca tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa
     5401 ccgcaggacg agatgttcgc ttcggttaaa aaaaggcacc ttacgaagtg cctctgataa
     5461 ttagctaagt gcatgttgtt catatcggat gcggcgtgaa cgccttatca aacctacaaa
     5521 acatttatat tcaatatgtt gcaatgaccg cgcaggccag ataagcgtag cgcatctggc
     5581 gattttgcgc ttgtcatcag tcttagcgcg ccttcacagc actttattaa ttccttgaac
     5641 gaatatttac tgccatttca ttcacgtcta ttcttaattt gctgctttaa aaacaagtga
     5701 ttgagcaaca ttgaggcaaa aatggactgg tatctgaaag taataaaaaa ttatgtcggt
     5761 ttccggggtc gtgcgcggcg caaagagtac tggatgttta ttctggtcaa catcatcttt
     5821 acgttcgtgc tggggctgct ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc
     5881 atcctgacga caatctacgg tattctggtg tttttaccgt ggtgggcagt tcagttccgc
     5941 cgcctgcacg acaccgaccg ctcggcgtgg tgggcactgc tattcttaat cccgtttatc
     6001 ggctggctta ttatcatcgt ctttaactgc caggcgggta cgccaggcga aaaccgcttt
     6061 ggacctgatc caaaactgga accttaaaac aaatctgctc gccggatgtc tgactcatcc
     6121 ggctttta
//
LOCUS       DAZOZD010000236         6115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOZD010000236 DAZOZD010000000
VERSION     DAZOZD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.239"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..353
                     /locus_tag="ACHUPB_003689"
     CDS             <1..353
                     /locus_tag="ACHUPB_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0760399.1"
                     /translation="RNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYT
                     SQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
     gene            complement(393..1064)
                     /locus_tag="ACHUPB_003690"
     CDS             complement(393..1064)
                     /locus_tag="ACHUPB_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR0760400.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1171..1404)
                     /gene="yedF"
                     /locus_tag="ACHUPB_003691"
     CDS             complement(1171..1404)
                     /gene="yedF"
                     /locus_tag="ACHUPB_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0760401.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1401..2606)
                     /gene="yedE"
                     /locus_tag="ACHUPB_003692"
     CDS             complement(1401..2606)
                     /gene="yedE"
                     /locus_tag="ACHUPB_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0760402.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2793..3206
                     /gene="yedD"
                     /locus_tag="ACHUPB_003693"
     CDS             2793..3206
                     /gene="yedD"
                     /locus_tag="ACHUPB_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0760403.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3240..4727)
                     /gene="amyA"
                     /locus_tag="ACHUPB_003694"
     CDS             complement(3240..4727)
                     /gene="amyA"
                     /locus_tag="ACHUPB_003694"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0760404.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4805..5170)
                     /gene="fliT"
                     /locus_tag="ACHUPB_003695"
     CDS             complement(4805..5170)
                     /gene="fliT"
                     /locus_tag="ACHUPB_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0760405.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5170..5580)
                     /gene="fliS"
                     /locus_tag="ACHUPB_003696"
     CDS             complement(5170..5580)
                     /gene="fliS"
                     /locus_tag="ACHUPB_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0760406.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5595..>6115)
                     /gene="fliD"
                     /locus_tag="ACHUPB_003697"
     CDS             complement(5595..>6115)
                     /gene="fliD"
                     /locus_tag="ACHUPB_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0760407.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1498 a         1605 c         1523 g         1489 t
ORIGIN      
        1 ggcgcaatgt ccgggcaaaa tcaggtttaa accgttcgat actggatcag ggctggtatg
       61 aaatgcaccg ccagcttgag tacaagcagc tctggcgtgg cggtcaggtg cttgctgttc
      121 cgccagcgta tacaagccag cgttgcgcgt actgtggtca tacagcgaaa gagaaccgcc
      181 tgtcacaaag taaattcaga tgccaggtat gtggatatac agcgaacgcc gatgtaaatg
      241 gtgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggagag atggtgcagt
      301 caggccgccc gttgaagcag gaacccaccg aaatgattca ggcgacagcc tgaacgtagc
      361 agggatcctc gtccttcagg gcgtggagga tgtcaacaaa caggttgaat taactccgcg
      421 ccctggtttt tgacattacc taccgcgcgc gataccgggt gccaggtgaa ctggtttgct
      481 ggcacacagc cgttggtagc aatttctgat gcttctttac cgccaatctc ttgccgcatc
      541 cattcctgtg cagcttctgg cgacagaacc agcgggcggc ggtcatgaat gtctaccaga
      601 ccctggtctg ctgcagcagt gacaattaaa aatccttcgg cttcgtcacc acgctcaaat
      661 ggtgtgctac ctatcgcggc cataaaaata ggttgtccat cagcacgata gataaaaaaa
      721 ggctgttttt tgtcgccttc ttttttccac tcaaaccagc catcggcaaa acagattgcc
      781 cgaccatgtt gccagagcgg tttaaacatg cgactggttg cggcagtttc tacgcgggcg
      841 ttaatcagcg gcggtttatc ccaccatccg ggagcatatc cccagaaaac cggatccaga
      901 tgaaggtgtt catcacgttc actgagcaga agaactttgg ttcccggcgc gacgttgtat
      961 ctgccaatgg gttcgggatc gtagggaata tcgcgttcaa tatcttccgc gagaagcgca
     1021 aggtaatctt cacgcgtttg ggattgggca aagcgtccac acatagaaac ctcctgagat
     1081 catactgaaa agaataggag agggggcggg taaaatctat gtgtcagagg caacaatgtt
     1141 cccgctgtgg gccacagcgg gaatgaggga ttacttctga attaaataac gaatggtcgg
     1201 cccgtcttgc tgaatatcca gcaccgtata gccgtgatta cgcgcatcca gtggaatatt
     1261 attgatcgac tgtggacagt cgctcaccac ttccaggatt tccccttttt ttaactgcgg
     1321 catcgcctca agggttgcga ctgccggata agggcagggt tcacccacca tatcaaggcg
     1381 gtaatcagga acgatatttt tcatgcgatc tccttagcag tctgcggtgc cgcacggcgg
     1441 aagaagcgtt tttcccagcc gataatcaac atcagcgcag taaacaacag caaatatgtc
     1501 accagcaggc cacccatcgg gccaaaggtt ttcagcaggt tgattttgtc ccagtcggtg
     1561 gccagcgccg gagcgaaatc atcccagtaa tacgccagaa tcgtcgagcc gatcacattg
     1621 cccagaccga cccaccagta gtgcacctgg ccttctaccg cgcggtacat ccagccggtt
     1681 tcgcagccgc ctgccagcac gatgccaaaa ccaaacagta aaccaccaat taccgcgttt
     1741 ggtcccgccc acatgatttt gggttcaacg cctaactgta cgtaactgaa gatcccgatg
     1801 gcactcaccg ccataccgat aatgattgct ttcgccatat gggtacgtcc ggtgatccac
     1861 atatcgcgga acgccgaagt aaagcagatt tgcgcacgtt caatcagtaa accaaagccg
     1921 acgccaaaca gcattgccag ccccagtttg ggttggttca tcgccgtgag cagcgcccag
     1981 cccagcatgc cgaaaaagac cagcatcccg agacgaaaac gacgccgcgc ctgatccggt
     2041 ttttgcgtca gcggtgaggc ggcagaaact ttctgcattt tcacgggaat acggaagatg
     2101 ggcagcaggg taaagcgcgc gccaaaccat gaaccaatgg cagtggcgat ggcaaagaac
     2161 caggcatgca gcgagaactg aggaatgccg gtaaagaacg ccgccaggtt acagcccatt
     2221 gccagacgcg cgccaaaacc ggcgataatg ccgccaataa tggcctgcat aatgcggata
     2281 cggctgcgcg gcatgcgcag tttgacattg ttggcccaca gcgctgcggc aaagcagccg
     2341 ccaaacatgc cgaggatcat catcccgtcg atgcgggtta atggcgatcc ttccagatgg
     2401 ataattttaa aataacccca ctcttcagca tggacgccaa acagctgcag gagctgaccg
     2461 ccccaacggg taaattcacc cgtgacagcc caaaaggtgc cagtaatgcc aaaatagtaa
     2521 gtggagagaa tacccgccgc gatgaccgca gggatgggtg cccagaattt aatcaaccag
     2581 gcgtgtttga attgctgcca tgacatgaat gtagcctctg aactcagaag gtttgaaaca
     2641 agaatcgcga atcatacatc atgcaaattt attttcgggg taataattca caaaaataat
     2701 agttagatca aatttctctc tgaacctgga aaggctggcg acatcagggc tttaatgcca
     2761 caatcctctt ttcttctcat gcaggacgaa tgatgaaaaa actcgcaatt gcaggtgcgc
     2821 tcatgttgct ggcgggctgt gccgaagtcg aaaactataa caatgtcgtg aaaacccccg
     2881 cgccggactg gctggcgggc tactggcaga ccaaagggcc gcaacgtgcg ctggtcagtc
     2941 cggaagagat aggcagcctg attgtcacta aagagggcga taccctggat tgccgtcagt
     3001 ggcagcgggt gattgccgtg ccgggcaagt tgacattgat gtctgatgat ctcactaacg
     3061 ttaccgtaaa acgtgagctg tacgaggttg agcgtgacgg taacaccatt gagtacgatg
     3121 gtatgacgat ggagcgtgtg gatcgcccga cggcagagtg tgccgccgcg ctggataaag
     3181 cgccgttacc gacgccactg ccgtaatgaa atctccccgg cttgccgccg gggatgaatt
     3241 taaatcacct cttcgataac ccacacgctg acgctgccgc cgttgcaaaa gaaggtcgct
     3301 tcgccgtttt cgtcggtcac tacgctctct tgccgattcc ccagaaaatc acgccaggtt
     3361 ttattgccgt aattctctcc cagatgaatg gttttttcgc catcatcccc gttcgacatg
     3421 accaccacgc agccgggata ttcgtcagtg ccactgcggc taaaggcaat gcagttcgga
     3481 tggtcgaaaa ataacgtctg tacaccgtgg gcgaaacgct gacgggcgag aattaactca
     3541 tcaagctgtt cgattattgg catatctatc ggataggttt gcccgtcacc accgacatct
     3601 tcgtaatgcg caccgtagag gtccggatag aacaccgaag gaacgccatt ttcccgcaac
     3661 aaaattagag catacgccag cggtttaaac cacggttcga ccggcgcttc gagggcttgc
     3721 aacggttggg tgtcgtgatt ggctacgagg gttacggcgt ggaaaggatc ggcttccact
     3781 aatgtacccg tgaaaatctg cgtcatgtcg tagtcgcgtc ccatgcgcga tgcttcatgg
     3841 aatttcatct gcagcggcgc atcgaacagc atggttttgc cttccacctg atcaatatac
     3901 gtttgcagtt tatcgacttc atgcgaccag tactctgcca caataaacag cggctttggc
     3961 gcaacttcct gtacgtgttc gatccactct ttataaaacc acgccggaat atgtttaacc
     4021 gcatcaagac gaaaaccgtc gcattgtgtt tgttccatca cccagcgcgc ccagtattta
     4081 atctcttccg tcacggcatg attgcggaaa tcgatattct cacccatcag ataatcgaaa
     4141 ttaccgagtt catcatcaac ctgatcgttc cagccttcgc cggtgtagtc gttaacaatt
     4201 ttaaatatgc catcttcatc aggattttcg atatggtcga taccgctaaa acatttgaag
     4261 tcccagataa actgcgagta ttgcccggca cgggcgggga aggtgtaacg cgtccagcct
     4321 tcgcactcaa tgatttcttc atcaatttgc gtacggtcat cagcatttac acgctgcacg
     4381 cgaatagctt ctttttcatc cgcccccatt ttgtggttga ctaccacgtc cagtagcacc
     4441 gcaatgtcat tacgtttcag agcgtcaatg gcggccagca gttgtgcttt atcgccatat
     4501 ttagtaggga tgctgccttt ctgatcaaac tcacctaaat caaataaatc ataggagtcg
     4561 tagccgaccg aatacccgcc cgatgcgcct ttataggcgg gcggcaacca gaccatattg
     4621 ataccaatat cattaaaacc gtcggcgcgc tcggccagtt caggccagag cttaccgcct
     4681 tccgggtaat accagtgaaa acattgtaac agcgtgggat tacgcattct tcgactccat
     4741 tcaaggggaa cattagaagc gtagccgtaa tcggattatt cgcgagccat cggctcgtta
     4801 agattcaaaa gaggttatcc tgcggagcca gcacaaagcc gccctgatct ccataggcac
     4861 ttaacactga tttttgcacc gatgactgac cgaccagttt cgccagttca tccatacgaa
     4921 tctgtaataa ctgctttacc ttgctttcgt tgtcgagaat caggcgcagc atcgggcgga
     4981 gctgctcctg catcgtggtg gacggggcaa cctcttcagt cagatgtgca atctcctgca
     5041 ccgcattcac atacgccatt tcgctggcga tgagttcgtc ccattgttct tccgttgcca
     5101 ggcgtaacat gagctggctt ttatcgacga gttgttgcca ggcgaaatac aaatgcggtg
     5161 catggttcat cagactgggt cctgaatcaa agaaggggag agtaaagact ctttccaggc
     5221 atcggcaata ttgcgcatta atgcttccac ttcttcgact gcggagacat cgttgcgtaa
     5281 attggcttgc agcaagcgcc tgaccatata gctataaaga gcaatcaagt tttgggttag
     5341 ttcgtctttg ctctcttcat caagactcac ccgcagcccg ttctcaatga tgttgatcgc
     5401 ttttgacaaa gagacgcctt tgccttgctg attgttgtcc tgcataaaca ggcgcgctct
     5461 aaccagtgcg ctcagcactc catcaaatag catggtgacc agctgttgct ggctggcgct
     5521 cattacggcg ctttcgacgc caatttgtgc ataggctttt gagccgctcg cggtatacat
     5581 gctgacctcc gtgatcagga attactcatt gcagtaaatt gttggctgag ataggtactg
     5641 gtgttattca gtttgctcat catggtatct aattgcgtaa actgtgcagt gtaacgcgcc
     5701 acggtgtcat caatgctggc actgacggat agatactgtt tggtcagttt tttcagtgtg
     5761 gcgttaatac tgtcctgtgc gctatcaata atgccatcat cggcaaggta accttttacc
     5821 tcggtggcaa ttttggtggt aattcctgtt tctttaccat cgcctaccag caattcacgt
     5881 acggatgctg tattttcatt caggactttt ttgagtttat catcatcaat tttcagctta
     5941 cccgtcgtgc cgtcctgctt aatcccgatc tcgtttaatg ttttgaaggc actgtcgctg
     6001 gcaccgtttg caaattgtgc gcggatgccg ctttgaattg tacgtacaac gctgtctccg
     6061 agaagtgcac cattattttt atcttgtgct tcagcacctg ggtctacttc tttat
//
LOCUS       DAZOZD010000237         6097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOZD010000237 DAZOZD010000000
VERSION     DAZOZD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.240"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUPB_003698"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="ACHUPB_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR0760408.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUPB_003699"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="ACHUPB_003699"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0760409.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="ACHUPB_003700"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="ACHUPB_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0760410.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUPB_003701"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="ACHUPB_003701"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0760411.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1618 a         1617 c         1450 g         1412 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtacttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccg
//
LOCUS       DAZOZD010000238         6063 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOZD010000238 DAZOZD010000000
VERSION     DAZOZD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.241"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUPB_003702"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUPB_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0760412.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUPB_003703"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUPB_003703"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0760413.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUPB_003704"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUPB_003704"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0760414.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUPB_003705"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUPB_003705"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0760415.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUPB_003706"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUPB_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0760416.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1437 a         1530 c         1646 g         1450 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaat atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 cta
//
LOCUS       DAZOZD010000239         6024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOZD010000239 DAZOZD010000000
VERSION     DAZOZD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.242"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            221..501
                     /locus_tag="ACHUPB_003707"
                     /pseudo
     CDS             221..501
                     /locus_tag="ACHUPB_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(600..1136)
                     /gene="ssb1"
                     /locus_tag="ACHUPB_003708"
     CDS             complement(600..1136)
                     /gene="ssb1"
                     /locus_tag="ACHUPB_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0760417.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1390..4212
                     /gene="uvrA"
                     /locus_tag="ACHUPB_003709"
     CDS             1390..4212
                     /gene="uvrA"
                     /locus_tag="ACHUPB_003709"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0760418.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4247..4603)
                     /locus_tag="ACHUPB_003710"
     CDS             complement(4247..4603)
                     /locus_tag="ACHUPB_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0760419.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4607..5023)
                     /locus_tag="ACHUPB_003711"
     CDS             complement(4607..5023)
                     /locus_tag="ACHUPB_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0760420.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5134..5847)
                     /gene="aphA"
                     /locus_tag="ACHUPB_003712"
     CDS             complement(5134..5847)
                     /gene="aphA"
                     /locus_tag="ACHUPB_003712"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0760421.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1424 a         1577 c         1570 g         1453 t
ORIGIN      
        1 taatgccact gaaaaaccac ataatctgta cagcaataaa agtcacggcc taatatggct
       61 aacaaacaat caattgtgat atagctcaca aaattaatga aacaaacaga gtgtttcatt
      121 tttgtgttct gcaagccccg cgatagccag agtgtaccac gcctcccgtg aacaacgccg
      181 cgctgtccag ggcatcggct cttttcagag gaattgtttg atggctaccc tcaccactgg
      241 cgtggttctt cttcgctggc agcttcttag tgccgtaatg atgtttctgg ccagcacatc
      301 aacatccgtt ttcgtcggtc tgattatgtc gggcttgcgg tgatcagcag cggtctgggc
      361 gttgtttccg cctgttggtt cgcaatgggg ttgcttggca tcacaatggc ggacatcacc
      421 gctatctggc acaacatcga gtcggtgatg atagaagaga tgaatcagac accgccacaa
      481 tggccaatga ttttgacctg atacgtagaa gcctccataa cggaggcttc ttttttatgg
      541 ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt aatgacaaat
      601 cagaacggaa tgtcatcatc aaagtccatc ggcggctcgt tagacggcgc tgccggagcg
      661 gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccaccctg cggctgctga
      721 ggctgccccc aaccgccctg cggctgacca ccaccgatat taccacctgc cggagcgcca
      781 ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac gacttctgtg
      841 gtgtagcgat cctgaccgga ttgatcggtc catttacggg tacgcagctg accttcgata
      901 taaacctgag aacctttacg cagatattcg ctggccactt ctgccagttt gccgaacagc
      961 acaacgcggt gccattcagt ctgttctttc atctcgccgg tcgctttatc acgccaggat
     1021 tcggaagtag ccagcgtaat gttggcaact gcgccaccat ttggcatgta gcgtacttcc
     1081 gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct ggccatgttc
     1141 gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac cattaccaat
     1201 agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag acattgttac
     1261 acaacactcc ggataatgca ttccaatact gtatattcat tcaggtcaat ttgtgtcata
     1321 attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg tttaatccgg
     1381 gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct caaaaacatc
     1441 aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg ttctggcaaa
     1501 tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt tgaatccctt
     1561 tccgcctacg cgcggcagtt tctgtcactg atggaaaagc cggacgtcga tcatattgag
     1621 gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc gcgttctacg
     1681 gtggggacaa tcaccgaaat ccacgactat ttgcgtttgt tgttcgcccg cgtcggcgaa
     1741 ccgcgctgtc cggaccacga cgtcccgctg gcggcgcaaa ccgtcagcca gatggtggat
     1801 aacgtgctgt cgcagccgga aggcaagcgt ctgatgctgc tcgcgccaat cattaaagag
     1861 cgcaaaggcg aacacaccaa aacgctggag aacctggcaa gccagggtta catccgtgct
     1921 cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact gcaaaagaaa
     1981 cacactattg aagtggtggt tgatcgcttc aaggtgcgtg acgatcttac ccaacgtctt
     2041 gcggagtcgt ttgaaaccgc gctggagctt tccggtggta cagcggtagt ggcggatatg
     2101 gacgacccga aagcggaaga gctgctgttc tccgccaact tcgcctgccc aatttgcggc
     2161 tacagtatgc gtgaactgga gccgcgactg ttttcgttta acaacccggc aggtgcctgc
     2221 ccgacctgtg acggccttgg cgtacagcaa tatttcgatc ctgaccgcgt gatccaaaac
     2281 cccgagctgt cactggctgg cggtgcgatc cgtggctggg atcgccgcaa cttctattat
     2341 ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc gccgtggggc
     2401 agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt ctggcaaaga aaacattgaa
     2461 ttcaaataca tgaacgatcg tggcgatacc tccattcgtc gtcatccgtt cgaaggcgtg
     2521 ctgcacaata tggagcgccg ttataaagag acggaatcca gtgcggtacg tgaagaatta
     2581 gccaagttta tcagcaatcg cccatgcgcc agctgcgaag gaacgcgtct gcgtcgggaa
     2641 gcgcgccacg tgtatgtcga gaatacgccg ctgcctgcta tctccgacat gagcatcggt
     2701 catgcgatgg aattcttcaa caatctcaaa ctcgcaggtc agcgggcgaa gattgcagaa
     2761 aaaatcctta aagagatcgg cgatcgtctg aaattcctcg ttaacgtcgg cctgaattac
     2821 ctgacacttt cccgctcggc agaaacactt tccggcggtg aagcccagcg tatccgtctg
     2881 gcgagccaga ttggtgcggg cctggttggc gttatgtatg tactggacga gccgtctatc
     2941 ggcctgcacc agcgcgataa cgagcgcctg ttgggtacgc ttatccatct gcgcgatctc
     3001 ggtaataccg tgattgtggt ggagcatgac gaagacgcaa ttcgcgccgc tgaccatgtg
     3061 atcgatatcg gtcctggtgc gggtgttcac ggcggtgaag tggtcgcaga aggtccgctg
     3121 gaagcgatta tggcggtgcc tgaatcgttg accgggcagt acatgagcgg taaacgcaag
     3181 attgaagtgc cgaagaaacg cgttccggcg aatccagaaa aagtgctgaa gctgacaggt
     3241 gcacgcggca acaacctgaa ggacgtgacg ctcacgctgc cagtcggtct gtttacctgc
     3301 atcacagggg tttcaggttc cggtaaatcg acgctgatta acgacacact gttcccgatt
     3361 gcccaacgcc agttgaatgg tgcgaccatc gccgaaccgg caccgtatcg cgatattcag
     3421 gggctggagc atttcgataa agtgatcgat atcgaccaaa gcccaattgg tcgtactcca
     3481 cgttctaacc cggcgaccta taccggcgtg tttacacctg tgcgcgaact gtttgcgggc
     3541 gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt tcagctttaa cgtccgtggc
     3601 gggcgctgcg aagcctgtca gggcgacggt gtgatcaaag tggagatgca cttcctgccg
     3661 gatatctacg tgccgtgcga tcagtgcaaa ggtaaacgct ataaccgtga aacgctggaa
     3721 attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga agaggcgcgt
     3781 gagttctttg atgcggtgcc agctctggcg cgtaagctgc aaacgttgat ggacgttggt
     3841 ctgacgtaca ttcgcctggg gcagtccgca accacacttt ctggtggtga agcccagcgc
     3901 gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta tattctcgac
     3961 gagccgacca ccggtctgca cttcgccgat attcagcaac tcctcgacgt gctgcataaa
     4021 ctgcgcgatc agggcaatac cattgtggtg attgagcaca atctcgacgt gatcaaaacc
     4081 gctgactgga ttgtcgacct gggaccagaa ggcggcagtg gcggcggcga aatcctcgtc
     4141 tccggtacgc cagaaaccgt cgcggagtgc gaagcttcgc atacggcacg cttcctcaag
     4201 ccgatgctgt aatcgttaag gccgctttct gagcggcctt ttcctttcag agttgtacca
     4261 gcaatttacg cttttcttcc ggtagtaaat tcactgcctg ctgataagac gcatccacca
     4321 gataatagat ttgcgaatcc ggcagcgaac cgtcgagata cacggtgctc cagtgcgctt
     4381 tattcagatg gcggctagga cgcacatcgc tgtgctgctg acgtagcagc tccgccagct
     4441 ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc atcgcaaaca
     4501 gtacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc tgttctgcgc
     4561 ctggttttgc catgcaatat tgtagcaact ccgaaatggt cattttttac tccccttgta
     4621 gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc cagatgcctt
     4681 gccaggtgcc ggtctgaata cgccctttat gtaacggcaa tacaagcgat gttcccagca
     4741 ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc tcataatttc
     4801 cgttgtcggg aacggtgcgg aggaaaaaac gctccatgtc gtggcgtacg gtgggatcgc
     4861 agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc agtaagccga
     4921 tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc agatgaaacc
     4981 cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc tccttgataa
     5041 aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat taaaaaaaca
     5101 gcattcggga gagcatcacg ctctcccgct ctgtcagtat tctgaattga cgatcacctc
     5161 ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg cgcgcagaat
     5221 gcggatacca cgagcgccga catcgcgtgc ggcggtaata tcgttatcgg aatcgccata
     5281 aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgcc ctggtttatc
     5341 gcccgcaaag attaccggat tcatgttggt ggcaggaata tgaaaattat ccgccagcgt
     5401 ttttgaaacc gtttctgttt tcgtcgggct acgaccagtc acaaagaaga tcgcgtcacc
     5461 gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc tgaattcatc
     5521 ccagccatta ttcatttttt cccagaacac aggatttttc agataatctt cgctttctgg
     5581 cgagaaggtt tttttgccgc gccagaaccc cggactggaa aaaagtaccg tgtcatcgat
     5641 atcaaacccc accgccattg gcggacgccc tgcgaggcta ttttcaattt gtgcgaccga
     5701 aacccaatga attggtgcct gttcagcaag cctggcaacg ttagtcccag ggttaagcgg
     5761 tgaaggagat gaggccaggg caacagcgga actgtttaga gcgaacaata agcaaacggc
     5821 actgattgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat gttgaaactt
     5881 tttattgtga gatttgttgc aaaaaccatc tgaccataac gccagctgcc ggggaaagga
     5941 aggattatct gcggtttttg tgatgaaaag caggatgatt atatttaacc cagtaaatac
     6001 gttcctgtct ttttttgtat aaaa
//
LOCUS       DAZOZD010000240         6015 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOZD010000240 DAZOZD010000000
VERSION     DAZOZD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.243"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(369..1304)
                     /locus_tag="ACHUPB_003713"
     CDS             complement(369..1304)
                     /locus_tag="ACHUPB_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0760422.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="ACHUPB_003714"
     CDS             complement(1802..1945)
                     /locus_tag="ACHUPB_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR0760423.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="ACHUPB_003715"
     CDS             complement(2029..2286)
                     /locus_tag="ACHUPB_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HFR0760424.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="ACHUPB_003716"
     CDS             complement(2296..3933)
                     /locus_tag="ACHUPB_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR0760425.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="ACHUPB_003717"
     CDS             complement(4154..4687)
                     /locus_tag="ACHUPB_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760426.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="ACHUPB_003718"
     CDS             complement(4690..5616)
                     /locus_tag="ACHUPB_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0760427.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="ACHUPB_003719"
     CDS             5792..>6015
                     /locus_tag="ACHUPB_003719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760428.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1488 a         1659 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agacaaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAZOZD010000241         5961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOZD010000241 DAZOZD010000000
VERSION     DAZOZD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.244"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUPB_003720"
     tRNA            292..368
                     /locus_tag="ACHUPB_003720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUPB_003721"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUPB_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0760429.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUPB_003722"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUPB_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0760430.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUPB_003723"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUPB_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR0760431.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHUPB_003724"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHUPB_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAZOZD010000242         5947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZOZD010000242 DAZOZD010000000
VERSION     DAZOZD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.245"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(130..876)
                     /gene="cutC"
                     /locus_tag="ACHUPB_003725"
     CDS             complement(130..876)
                     /gene="cutC"
                     /locus_tag="ACHUPB_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0760432.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(890..1456)
                     /locus_tag="ACHUPB_003726"
     CDS             complement(890..1456)
                     /locus_tag="ACHUPB_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0760433.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1672..3408
                     /gene="argS"
                     /locus_tag="ACHUPB_003727"
     CDS             1672..3408
                     /gene="argS"
                     /locus_tag="ACHUPB_003727"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0760434.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3450..3494)
                     /gene="yecV"
                     /locus_tag="ACHUPB_003728"
     CDS             complement(3450..3494)
                     /gene="yecV"
                     /locus_tag="ACHUPB_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR0760435.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3585..3947
                     /locus_tag="ACHUPB_003729"
                     /pseudo
     CDS             3585..3947
                     /locus_tag="ACHUPB_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(3951..5021)
                     /locus_tag="ACHUPB_003730"
                     /pseudo
     CDS             complement(3951..5021)
                     /locus_tag="ACHUPB_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(5014..>5947)
                     /gene="flhB"
                     /locus_tag="ACHUPB_003731"
     CDS             complement(5014..>5947)
                     /gene="flhB"
                     /locus_tag="ACHUPB_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR0760436.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1535 a         1584 c         1475 g         1353 t
ORIGIN      
        1 cccgctcaac agatcacggt tatcgttcgt tttttatact gctcagggat aaaaaaaggc
       61 ccctgttgaa attgcagggg cctggtacga gcaagcatca tattgggcga catgatgcaa
      121 cggtaaaaat catttggcct gatggcgttc aatgattcct ttcatttcag caaccgccgc
      181 cccgtctacg atataacgcg aatactcgtc cgcgtgttca tctgatgaca tggacaatcc
      241 ttgattacga taacgcatcg gtgaggcttg ccacgctccc gcggagctat ggacttccag
      301 cactccggca tcgaggaagt ggtgcaggtt ttctgcacgg actcctgctc cggccataat
      361 gattggagca tcacgatggg caataagttc cataattttt gataaacctt gcagtgcgtc
      421 tgatttttgc cctgatgtca gtacccgggc aatgcccaat tccgcaagat tattgagtgt
      481 atataaaggg ttagcgcaca tatcgaaggc gcgatgaaaa gtcactgcca gcggaccggc
      541 agcagccatt attttttcca ttcgtggcat atcgacattc ccgtcaacat cgagaacgcc
      601 cgtcaccagt ccaggaaaac ctaattcgcg gaccgtgcgc acatcctcaa gaatggcggc
      661 aaactcaccg tcgctgtagc aaaaatcacc accgcgtggg cgaattatcg gatgcacagg
      721 gatcgtcacc cgctggcgca cggatttcag tacccccaac gacggcgtta agcccccctc
      781 ttttggggct gcgcataatt caactctgtc tgcgccgttt tgctgcgccg ttagtgcaca
      841 ttccatgctg taacagcaaa tttccagtaa tgccattttt gctccttaat tacgctgact
      901 gctcgctggc aacgatctct tcaatggacc acggatgaaa cttaatagtt gttttgccat
      961 cggtgaccgc cagcgtcgga ttcggtaatc gctcatgctc accttttggt gaactggttt
     1021 taactgagat ccccggtagg ctgagtcctt catcagaaag caatgccagc gcacgggcat
     1081 tcagcgtttc tggatcaccc agcagaacga tttcaatatg ttcccaacct tcgtgtgggt
     1141 aacgtttttc cccgggccac ggtagctcca caatagaaaa ctgccagtgc gcaacctgta
     1201 ccggttcatg caatttaaac agacaaatcg gtctgccatt gatcatattt tctgacaaaa
     1261 gctcgccaca ctgttcaaac ccgcgacgcc agcgttcagc agtggcgttt tgatggcaac
     1321 gcaaagaaat gtgatcggca gtcagcggag tgatattcaa ccccagacgg agggaaagtt
     1381 catctaatgc gtgggtaaat cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag
     1441 attgccagtt cgccataatc actcttcgtc tttcagtaaa agcgttaatt taccctgttg
     1501 ccctgcgcca accaaccgct gatttcacgc cgcttctgat gcaatagtga aaacggcaat
     1561 acgccacgcg cacgttgctg acgaaaacag ccatttgcag tatactcccg ccctaatttc
     1621 tttaactggt gcgggcaatt tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt
     1681 caggctcttc tctcagaaaa agtccgtcag gccatgattg cggcaggcgc gcctgcggat
     1741 tgcgaaccgc aggttcgtca ttcagcaaaa gttcagttcg gcgactatca ggctaacggc
     1801 atgatggcag ttgctaaaaa actgggtatg acaccgcgac aattagcaga gcaggtgctg
     1861 actcatctgg atcttaacgg tatcgccagc aaagttgaga tcgccggtcc aggctttatc
     1921 aacattttcc ttgatccggc attcctggct gaacatgttc agcaggcgct ggcgtccgat
     1981 cgtctcggtg tttctacgcc agaaaaacag accattgtgg ttgactactc tgcgccaaac
     2041 gtggcgaaag agatgcatgt cggtcacctg cgctctacca ttattggtga cgcagcagtg
     2101 cgtactctgg agttcctcgg tcacaaagtg attcgcgcaa accacgtcgg cgactggggc
     2161 actcagttcg gtatgctgat tgcatggctg gaaaagcagc agcaggaaaa cgccggtgat
     2221 gaaatggagc tggctgacct tgaaggtttc taccgcgatg cgaaaaagca ttacgacgaa
     2281 gatgaagagt ttgccgagcg tgcacgtaac tacgtggtaa aactgcaaag cggtgacgaa
     2341 tatttccgcg agatgtggcg caaactggtc gacatcacca tgacgcagaa ccagatcacc
     2401 tacgatcgtc tcaacgtgac gctgacccgt gatgacgtga tgggcgaaag cctctacaac
     2461 ccaatgctgc caggaattgt ggcggatctc aaagccaaag gtctggcagt agaaagcgaa
     2521 ggggcgaccg tcgtattcct tgatgagttt aaaaacaagg aaggcgaacc gatgggcgtg
     2581 atcattcaga agaaagatgg cggctatctc tacaccacca ctgatatcgc ctgtgcgaaa
     2641 tatcgttatg aaacactgca tgccgatcgc gtgctgtatt acatcgactc ccgtcagcat
     2701 caacacctga tgcaggcatg ggcgatcgtc cgtaaagcag gctatgtacc ggaatccgta
     2761 ccgctggaac accacatgtt cggcatgatg ctgggtaaag acggcaaacc gttcaaaacc
     2821 cgcgcgggtg gtacagtgaa actggccgat ctgctggatg aagccctgga acgtgcacgc
     2881 cgtctggtgg cagaaaagaa cccggatatg ccagctgacg agctggaaaa actggctaac
     2941 gcggttggta ttggtgcggt gaaatatgcg gatctctcca aaaaccgcac cacggactac
     3001 atcttcgact gggacaacat gctggcgttt gagggtaata ccgcgccata catgcagtat
     3061 gcttacacgc gtgtattgtc cgtgttccgt aaggcagaaa ttaacgaaga gcaactggct
     3121 gcagctccgg taatcatccg tgaagatcgt gaagcgcaac tggcagctcg cctgctgcag
     3181 tttgaagaaa ccctcaccgt ggttgcccgt gaaggcacgc cgcatgtgat gtgtgcttac
     3241 ctgtacgatc tggctggtct gttctctggc ttctacgagc actgcccgat cctcagcgca
     3301 gaaaacgaag aagtgcgtaa cagccgtctg aaactggcac aactgacagc gaagacgcta
     3361 aagctgggtc tggatacgct gggtattgag acagtagagc gtatgtaatc gatttttcgt
     3421 gagagtaaag cctgatcagg gttacctgat caggcttttt tattgccatc taaatgtatt
     3481 ctgaaaatgg acatgccatt gttttctgac tgttggataa gaggccagaa gcgtaatatc
     3541 cggccccagg gaaacgataa cggttgaatt taaggaatac cgcagtgttt aaatttcttg
     3601 tattaacatt aggcattatc tcttgccagg cttacgcaga agatacggtt atagaaaacg
     3661 accatgacat ttcagccatc aaagattgtt ggcaaaaaaa ttcagatgat gatactgaca
     3721 ttaacgtgat caaatcatgc ctgcgacaag aatacaatct cgtcgatgcg caattaaata
     3781 aagcctatgg tgaagcttat cgttatatag aacaagtgcc acgcacaggt gtaaaaaaac
     3841 ctgataccga acaacttaac ttgcttaaaa aatcacagcg agcctggctg gattttaggg
     3901 acaaagaatg tgaattaatc ctttcaaatg aggacgttca ggatttaatg tacatcgttc
     3961 cacgtcgctt cgacaacggt attattctct gccattttta ccggtttggg ttcggcaggc
     4021 gctttttgtt cgcgtccgcg tatccaccag gccagcccga gcaatccggc agtgaacagc
     4081 aaaaatacca ggttcggcat tccaggcacc aggccgagta aaccgagcac ggcggcgctt
     4141 aacaacataa cgcttgggtt actgaaaagc tgattcacca tctgctcgcc aacatcctga
     4201 tcggtgctga cacgcgtaac gatgaccccc gcggcggtaa aaatcaccag cgccggaatt
     4261 tgtgccacca gaccatcgcc aatggtcaat agcgtataac tttccgccgc gtgtcccatg
     4321 ctcatgccat gttgcagcac gccgaccagc aacccgccga caacgttaat gaccatgatg
     4381 aggatcccgg cgatggcatc gccgcgaaca aacttacttg ccccgtccat tgagccgtaa
     4441 aaatcggctt cctgagtcac ttcggagcgg cgttttttcg cctcatcttc accaatcaat
     4501 ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg gcataccatc gagaacaaag
     4561 cgcgcaccca cttctgcgat acgcccggca cctttggtaa tgaccataaa gttgatgatc
     4621 acgagaatga caaacaccac gataccgata gcgaaattgc caccaacgag gaagtgaccg
     4681 aacgcttcga ccaccttccc tgccgccgcc gcgccggtat gcccttccat caaaatgata
     4741 cgggttgaag ccacgttaag tgccagacgc aacaacgtag taaacaacag aatggtcgga
     4801 aacgcagcaa actcaagcgt gcgctgggta aacatcgcca ccagcaacac catgatcgac
     4861 aaggcaatat tgaaggtaaa caacaggtcg agtatgaatg cgggcagtgg cagcaccatc
     4921 atcgacaaga tcaacaggat caaaatcggt ccggcaagga tctgccattg tgtcgatttc
     4981 aggtttgcgg gcaggcgcag catcgcggcc agattactca tgggtcggtt tctcgttaat
     5041 aaaatccagg gcttccggca ccggaagatg agtaggttgt acagggcgct gtccaccagc
     5101 cagacgccag cgtttcagtt gccagaccca ggccagcact tccgccaccg cggcgtacag
     5161 ttgacccggg atttgttgac caatctccgc atgtcgatac agcgctcgcg ccagcggcgg
     5221 cgcttcaagc gtcgggacgt tattttcagc gccaatttca cgaatgcgca gcgcgaccag
     5281 ccctgcacct ttagcgacca ctttcggtgc gctcattttg ttttcgtcat actgcaacgc
     5341 taccgaatag tgggtcgggt tattgacaat gacatcagct ttcggcacat cggccatcat
     5401 ccgacgccgt gcagcagctc gctgcatctg acggatccgc cctttaacat gagggtcacc
     5461 ttcgctttgt ttgaactcat cacgaatatc ctgccgcgac atacgcagct ttttcaggtg
     5521 gctgaagatt tggaaaaaga cgtcaaatcc caccattgga atgacaccaa gcaccaccag
     5581 cagtgcgcat agcccaacca gatccatcgc attacccatg gcggtagtcg gagactcggc
     5641 cattaagcgc atcatctgcg gccagtgatg ccagagataa aaccccgtca cgctgccaac
     5701 caggatggtt ttcaaaattg ccttaagcaa ctccgcgcca gtctgtgccg agaacatccg
     5761 tttaatgccc ggtagcgggt tgagtttgga aaacttcggc tgcaaggatt tgccgctaaa
     5821 taccagcccc cccagcatga ccggagaaat gagcgccacc agcaccacgc cgctaatcag
     5881 cggcagcagc gccagcatgg cttctctgat cagcagaata atctgcccga ggatcagatt
     5941 cgggtca
//
LOCUS       DAZOZD010000243         5942 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZOZD010000243 DAZOZD010000000
VERSION     DAZOZD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.246"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(17..2449)
                     /gene="metL"
                     /locus_tag="ACHUPB_003732"
     CDS             complement(17..2449)
                     /gene="metL"
                     /locus_tag="ACHUPB_003732"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0760437.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(2452..3612)
                     /gene="metB"
                     /locus_tag="ACHUPB_003733"
     CDS             complement(2452..3612)
                     /gene="metB"
                     /locus_tag="ACHUPB_003733"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0760438.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3889..4206
                     /gene="metJ"
                     /locus_tag="ACHUPB_003734"
     CDS             3889..4206
                     /gene="metJ"
                     /locus_tag="ACHUPB_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0760439.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            4390..4998
                     /locus_tag="ACHUPB_003735"
     CDS             4390..4998
                     /locus_tag="ACHUPB_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0760440.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            5143..5818
                     /locus_tag="ACHUPB_003736"
                     /pseudo
     CDS             5143..5818
                     /locus_tag="ACHUPB_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1425 a         1674 c         1516 g         1327 t
ORIGIN      
        1 tattaaattt ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc
       61 cggcggtgac gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcgatacc
      121 agcggctttc gatggcaaag acgttatcgc acggcagcag tgatgccagc ggatggtctt
      181 cacgcaccgc ttccacgccc acacgcgctt tgccattggc atcgaaacgc gccacgtagc
      241 gcagcaccag ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt
      301 catcgccatt ttcaaagaaa tggtcgatgc tgccgccttc gcaatgggca ggcaccagcg
      361 actccacgcg tacctgatcc ggttcgatgt tgtaacctgc ttcacgcgcc agaatcacca
      421 gcttgcgcat cacgtctttg ccagagagat cgtcacgcgg gtcaggttcg gttaagccct
      481 gctgccacgc ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca
      541 accaggagag cgtgccggag aagatcccgc tgatcgacaa aatagtatcg ccgctgtcga
      601 tcagatcgcg cacggtgtgg ttgatcggta agcccgcacc gacggtggca ttgtacagcc
      661 agtgacgccc ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgctgg
      721 ctcccgccag tttgttggcg ctgatgacgt ggaaaccgtg gctggcgaaa tcaagatact
      781 gatcagcaag ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg
      841 cgcgcatcca caggaacaac gactcttcat cctgctcaac cgcttcatcg ttgaagaagg
      901 ctaacgcgcg gctggcgtcc agcccgtcat agctcaacag actgcggcgg ctgtccacca
      961 cgcctgccag cacaaattca aagtcggtac gtgccgaaag cgtgctctgc tcacgggcga
     1021 acagttccag ccaacgggaa ccgatattgc ccttaccgaa caataccagg ccgatgcgtt
     1081 tttctgcgcg gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc
     1141 gcagtactgc caccaggctg atgccgtcat cggactgcca ggtaaattcg accggctggc
     1201 ctttcagttg ctgccagaag cggtggcaat gcagcgggtt acgggtgacg cctgcaccga
     1261 ccatcgccac cagcgccagc ccctgacgca ggcgcagttc gccaggtaac cccgcttcgt
     1321 cgaggatttt cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc
     1381 gatcgttatg tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg atttggtcga
     1441 tctctttatg cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga
     1501 catcatcgtg gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc
     1561 gcgtggaacc ttgatccggc gtgtagctac agcgcagttg caggtcgatt tcgctgccag
     1621 aaaccggctg taaagtacgg gcgtgaagaa cgggagccgc cagacgagcc agttcgctgg
     1681 cttcatccag acgcagcaac ggcagcagac aggcatcttt cactttacgc gggtcggcac
     1741 tgtatacccc ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga
     1801 tttgtgtcgc ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt
     1861 tacggctgat aaacccggtc accaccagac gtttgcccgg atgttgcacc agtagctgtt
     1921 gcagcaacgg gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt tcagcgcgta
     1981 aaaactcgcg ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca
     2041 tcaggcgtgc cgaccatact tccccgtggc ccaccacttc cgcatacact gcgtcgttaa
     2101 taccgctgtc gagcagcgcc gccaggcgtt ccagatcgct gacaaaagcg ctaatgaggc
     2161 tatcggcttc ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg
     2221 tttgttgaac ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca
     2281 actggttagt ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact
     2341 ccgccataat gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa
     2401 atttatgcag ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt
     2461 gtttgcagcc cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc
     2521 ggtggagata cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat
     2581 gcctgcatgt gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc
     2641 cgccagcgta aacaacgaca gcccgctcag gaaacgacgc agcgtctgct catcgccatc
     2701 cagttcaaaa ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc
     2761 ctgattttcc ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata
     2821 tttcacaatc gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa
     2881 cccgcgtagc agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca
     2941 ccaggcaagt tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc
     3001 agagtgaccg ttcagatatt tcgtgcatga atgcaacacc agatcggcac ctaatgccag
     3061 cggattttgt aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc
     3121 ccttgccaga tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct
     3181 ttctaccagt accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc
     3241 ttgatcaaca aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata
     3301 gctaccgccg tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt
     3361 cgttaccagg tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc
     3421 cagttctgcc agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc
     3481 atgcgcgcgc ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg
     3541 gacaacgcaa ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg
     3601 tttacgcgtc atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt
     3661 aaacattgtg ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt
     3721 gagcaaatcc caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca
     3781 cgatgtcacg gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa
     3841 attgtgtggt ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg
     3901 agcggcgaat atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag
     3961 attacggttt ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt
     4021 caggtgaaca acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat
     4081 gcctttaccg ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa
     4141 atcccggaag cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa
     4201 tactaaagca aaaaagagcg gcgcggagtg gaatcgcctg atgcgctacg cttatcaggc
     4261 ctacgtcata ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac
     4321 gccgcatccg gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga
     4381 cgtttaacta tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt
     4441 gcctggcagc cacaaaccgg cgacatcatc tttcagatct ctcgctcatc gcaaagtaaa
     4501 gcgatccaac tggcgaccca ttccgattat agccacaccg gtatgctggt gatacgcaac
     4561 aaaaagccct acgtttttga agcggtcggc ccggtgaaat accccccgct caagcagtgg
     4621 atcgcccatg gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg actgagtgtt
     4681 gaacaacagc aaaaactggc gcaaacggca aaacgttatc tcggtaaacc gtatgatttc
     4741 agcttctcat ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag
     4801 aacgcgctgg gaatgcgcgt gggcgagcag cagaagttga aagagttcga tctcagcaac
     4861 ccgctggtcc aggctaagct caaagaacgc tacggcaaaa atattccgct tgaggaaacg
     4921 gtagtttcac cacaagccgt tttcgacgcg ccacaactca ccacagtcgc caaagagtgg
     4981 ccgctgtttt cgtggtaatg gatgatgaaa aaaataattc ccgccgtagt ctgtttgttg
     5041 ctttgtagtt gcacgacctt tccgcaagct gtcgctccgg taaacggtga tgcctccaat
     5101 tagttgaatt aaggatataa tactcgcttt taaggttgtt tcatggtcac agttaacctt
     5161 cattgccccc gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc aaaaagacat
     5221 gagcgattcc gcggccgtga ctgtcaccgg gtatttcagc tcagttactg ttacgagccc
     5281 cgtaaaccgg gagttaaaga ccaaatcgct gaaatgactt tcaacggtgc cggggttcgc
     5341 gatactgccc gtacgctgaa aatcggcatt aatacagtca tccgaacttt aaaaaactct
     5401 cgccacgaag aataacgtca tccccggtcg ctcatgctga tgtggcactc atctgcaaac
     5461 ttagtcagca atggagcttt gtcggcagta aagcgcgaca gcactggctc tggtacgtgt
     5521 acaacacgaa aactggtggt gttctggcct acacttttgg cccccggact gacgaaacct
     5581 gtcgtgaatt gcgggcgctg cttacccttt taccatcggc atgttgacca ctgacgaatg
     5641 gggtagttac gctagagagt taccgaagga gaagcatctg actggtaaga tttttactca
     5701 gcacattgag cgtaacaatc tgaccctgag aacccgaatc aaacgtctgg cccgcagaac
     5761 catctgtttc tcgcgttctg tagaacttca tgaaaaagtc atcggtgctt ttatcgaaaa
     5821 tatatgttct actaattgga gtcatcaccc ttctgataag ctttccttgg atgattacca
     5881 gactgaatac acagccaaag atttttacct cattctgctc ttttttctca aatgacatat
     5941 at
//
LOCUS       DAZOZD010000244         5846 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZOZD010000244 DAZOZD010000000
VERSION     DAZOZD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.247"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHUPB_003737"
     CDS             complement(38..400)
                     /locus_tag="ACHUPB_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0760441.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUPB_003738"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUPB_003738"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0760442.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUPB_003739"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUPB_003739"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0760443.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUPB_003740"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUPB_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0760444.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUPB_003741"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUPB_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0760445.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHUPB_003742"
     CDS             5321..5683
                     /locus_tag="ACHUPB_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0760446.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1473 a         1445 c         1519 g         1409 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgagat
     5821 tctgaaaatg catttttacc gaaata
//
LOCUS       DAZOZD010000245         5823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOZD010000245 DAZOZD010000000
VERSION     DAZOZD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.248"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            331..744
                     /locus_tag="ACHUPB_003743"
     CDS             331..744
                     /locus_tag="ACHUPB_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760447.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            746..1525
                     /locus_tag="ACHUPB_003744"
     CDS             746..1525
                     /locus_tag="ACHUPB_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0760448.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1705..2349
                     /locus_tag="ACHUPB_003745"
     CDS             1705..2349
                     /locus_tag="ACHUPB_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFR0760449.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2436..2744
                     /locus_tag="ACHUPB_003746"
     CDS             2436..2744
                     /locus_tag="ACHUPB_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFR0760450.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            3158..4138
                     /locus_tag="ACHUPB_003747"
     CDS             3158..4138
                     /locus_tag="ACHUPB_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR0760451.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            4131..4547
                     /locus_tag="ACHUPB_003748"
     CDS             4131..4547
                     /locus_tag="ACHUPB_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR0760452.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(4549..5823)
                     /locus_tag="ACHUPB_003749"
     CDS             complement(4549..5823)
                     /locus_tag="ACHUPB_003749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFR0760453.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRTLKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNYV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
BASE COUNT         1454 a         1327 c         1451 g         1591 t
ORIGIN      
        1 agcacaacat gtggtatctc attcagctgc ttatgacact atatgttgtg tcatctccct
       61 gacctgtgat gcgccgcgca ggggcggaaa acagcgatat gatgattttc tcagcgtggt
      121 acacttccgg aaagttgtga tattccggaa agtcggatct gacggaaacg gctctccggt
      181 aatttaacgg cgtggttata tggatgcttg ttatcatggt gatgatgata acggcatgat
      241 gttatctgac ggcgtgacgg taagggcagt gatgatggat gacgttatcg catgaccgtc
      301 cctgcccgga aaagaaaaaa ggagtcaccc atgtttttta ttgagaatga aggtcaggct
      361 gtcgccggaa cggattactg gcagtctgta caggcgcagg ccggatatgt ctacctcagc
      421 tggaatgccg gcgcagccag gctgcttgtc ccggatgcgg caaaacattt actcagggag
      481 atgcgggggg ctgagtacgt catcatcagt aagggagcac tgcatggccg cgatgcgctg
      541 gaactggtat ttgaagacgg cagcgatgcg ccgtttgtga tccacatgct gagtgagcag
      601 tgcgatcgcc tgctccccga aaacaaccag ggagggggtt ttgttgtcac cgtctggacg
      661 cgtggcggta accagctccg ttatccggga aagtaccgtg ttgtggaaaa cctgcccgac
      721 gtttccccgt ggagtgaaca ctgatatgca gcacctgccg gcaccgatcc accatgcccg
      781 ggatgccgct cagcttcctg ttgccatcga ttatccggca gcgctggcac tccgccagat
      841 gtcgatggtt catgatgaac tgcccaaata cctgctggcc cctgaagtga gcgccctgct
      901 ccattacgtc ccggatctgc gccgcaagat gctgctggcc acactgtgga acaccggtgc
      961 gcgcattaat gaagcactgg cgctgacgcg gggggatttt tcgctcacgc ccccgtatcc
     1021 gtttgtgcag cttgcgaccc tgaaacaacg gacggaaaaa gccgccagga cggcaggaag
     1081 aatgcccgcc ggtcagcaga ctcaccggct ggttccgctc tccgacgcct ggtacgtcag
     1141 ccagctgcag acgatggtgg ccacactgaa aatccccatg gaacggcgta ataaacgaac
     1201 aggcaggaca gagaaagcgc ggatctggga agtgacggac agaacggtca ggacctggat
     1261 tggggaggcg gttgccgccg ctgccgctga cggtgtgacg ttctctgtcc cggtcacgcc
     1321 acatacgttc cgccattcct atgcgatgca tatgctgtat gccggtatac cgctgaaggt
     1381 cctgcagagc ctgatggggc ataagtccat cagctcaacg gaggtctaca cgaaggtgtt
     1441 tgcactggat gtggctgcac ggcaccgggt gcagttttcg atgcctgagt ccgatgcggt
     1501 gtctatgctc aaaagaattc cataagctct gactttttta aatccctttc ggatatctca
     1561 tggatatatt atcggtattt catattctgt tgacatgcag gaccggtata gagtaaaaaa
     1621 acatctattg agtacctctt ggatatccga aaggtgtctt atggggtctt tatgggtatt
     1681 acatgtggat tctggagata cactatgcct gttattgcta atacgcatcc caaaggtggt
     1741 gtgggtaaga caacttcatc cgtaaacatt gttggtgaaa tgaagtctga tacagttgat
     1801 ctggatactc ataccgggct ttctatcatt ctggggctga gacctgaagg aaaagaaatt
     1861 tccgtgaaag tacccaaaac agtggatgag ttaatcgaaa ttatgactcc ctacaagaac
     1921 agcgataaga cgttacttat tgactgtggg ggatttgact cagatcttac ccgtactgcg
     1981 attgcctttg cagactgcgt tattgtccct tcaaaagact ccctgactga acgtattggt
     2041 ttgatgcatt ttgatggagt actggacgaa atcagctcta tcatggggac cgatataact
     2101 gctcacctgt atctctgtaa agttaatcct aacaagaaga aattccctaa gcttgatgca
     2161 atcctgccat cgttcaaaca tctgaagctg atgaagagcc gcatttctgc tcgtgcagaa
     2221 tttgaggatg tcattgagac tgggatggga atcactgagt ctgttcatgg gcgctattcg
     2281 gcaggaggta aggaagttat tgccctgatc gaagaaatca atcatctgat tgaaaataac
     2341 aaacagtaag gtgtgtctat tgggtatcca tatagtatcc tttgggcttt taatggatat
     2401 cttttaagta tctaacaaac atcctgggtt atctcatgag caaagcgaaa tttagtgcac
     2461 aggctgaaaa actgaaaaac ctggtggatt ctgccagatt acaaacttct tcttctgccg
     2521 taacagcaac agccccgtct cagccaaatg aaggtgcaga ggaagtttca ggtcgtcccg
     2581 tggctgccag gacgaaggct aagaatctta aggccatacc gctgtcgtat tttgaagctc
     2641 atgcggaact taaagcgacc agtaaaacat ctctggattt tagctcatat attattgagg
     2701 ctataagaga gaagcttgaa cgtgatggtg caataggtca ataaactatc ggatacctta
     2761 agggtatctg atgtgtgtct tttaaatgcg ttatggggat tcttcacgta cctaaagagt
     2821 ccccattaga catctttctg tttatggtga catgattcgt caccgctttc ctttcctctc
     2881 agtttcacca tttcattcct tttcccttaa cctgaaccat gatgtccacg atgtttttat
     2941 attgcattta tgtgtcacat ataaccttta taatgttttt gtatctatga tttcctctgg
     3001 tcttaaagtt gtgtgtacaa tgtacataca atttatcgtg tttttatgtt gcatttgtcc
     3061 tttcagaaag actgtttttt cattataatt gaatatacat tgcatgtatg aagtatgttt
     3121 gttttttcta tttgtttatg agcaacggag actggttatg aaaatcttta ttgatgatgg
     3181 ttctaccaac attaaacttg cctggttgga ggatggtgac gtgaaaacgt taatcagccc
     3241 taacagcttt aaaccggaat ggtcttttag tttactggat gatgctgctc cggctaatta
     3301 cgagatcgac ggcgagaagt tttcatttga tccattaagt gctgatgctg tcgtaacgac
     3361 tgaaacacgc tatcagtaca gtgatgtgaa tgttgtggcc attcagcatg cgctacagca
     3421 gaccggcctg aaagcgcagc ctgttgatgt catcgtcaca ttgccgatct ctgaatacct
     3481 tgacgccaac aatcagaaaa ataagcaaaa cattgaaaga aagaaaaaaa atgtgatgcg
     3541 tgaagtacgg gtgcagggga gtgatgcatt tgttattcgc agtgtttccg ttcttcctga
     3601 aagtattccc gctggtttca gtgttcttgc ggggcttgag gatgatgaat cccttttgat
     3661 cgtagatctc ggtggtacaa ctcttgatgt gtcgcatgtt cgtagcaaaa tgacagggat
     3721 tacaaaaacc tggtgtgatc cgaatatcgg cgtgtccctg attacatccg gggttaagga
     3781 acagatggct gtacatgcta atacaagagt gagctcattc caggctgaca atatcattgt
     3841 gcaccgtaat gaacctgact acctttcccg tcgcatttat aatgctgaac agcgtgagtc
     3901 tatcattaat gttatcaatg aacggcagaa gttgcttatt aaacgagtta atgatgtcat
     3961 cagccgtttc accgactata cacatgtaat gtgtgttggt ggtggggctg aaattgtggc
     4021 cgaggctgtg aagaacctga ctaaggttcc tgatgagcgc ttttatctga gctcatcccc
     4081 gcagtttgat ttagtcatgg gaatgataaa aatgaaaggt ggtgtgacta atgagtgatg
     4141 aaaataaatc cagacgctgc tcatttgaac ttttcccgga tgaacggaca ggagataaga
     4201 tagcagatga gctcattgca aatgaaaaac tgaaggagcg gggacgtttc atgcgcgcga
     4261 tgttagttac tggtgcggcg tttgcagcta tagataaacg tcttcctttg ctgatatcgg
     4321 agttactgac agaaaatacg actctggatg atattaataa ggtgatttcc agcgtaatac
     4381 caggtgcttt ttcggttgaa aagaaattgc ttgaattact ggaaaaacaa tctggtctcc
     4441 atacgtctgt ggattgcagt actcctctta cagagcagag tctgtcgaga aatgacggtg
     4501 aagaccagac cagaagaaac gctgaaaata tgttcggaga tgactgaact aacccagcct
     4561 ggccagggga atatctcgcc atttagttgt ataacactga ctcaacattt cccgtttcat
     4621 ttgccattca ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt
     4681 cagttcatcg agtgttttca ttaacttttc actgttttta cggggctgga tttcatcaaa
     4741 taatcccggc tgcgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat
     4801 atagcggtac ccttcacgcc agacatagtt taaggctctg catgcggcgg caataatgtc
     4861 ccggctgtcc tgtgtgggca atggaagctt ttccacagcg gcattgctgt aacagggttc
     4921 ttttactgca aagggggatg tccgtacaaa tgtcgtcacc tgccggcaat actgacgctc
     4981 cccacgtagt ttctctgcgg cccgctctgc atactgaaca acagcctggt gcatggcatc
     5041 tttgtctgtg attcgttcac caaaactgcg actacagaca atctgctgtt ttgccggtgg
     5101 tgcttcttcc agggatatgc aggactcgcc gttgagttcg cgtaccgtac gctcaagaat
     5161 gacgctgaag tttttccgga tgaatgccgt gttagcctgc gccagctgca gtgctgtgtt
     5221 aatacccagc gcattcagct tttccgtcag tctgcgtcct actccccaga cctcaccaac
     5281 tggctgcagt cccagtagct tcaaggtccg attacggttt tctgccgtca gcgcgaccac
     5341 tccggaaaac tgtggccatt gctttgttgc ccactgtgca cttttagcca gcgtttttgt
     5401 aggcgcaatg cccaccccca tggtgagtcc tgtccagctt tttacctgtt ccctgagctg
     5461 atgaccaaaa aactccggag agatgcaatg atttatcccc cgcaaatcaa taaacatttc
     5521 atcaattgag tagggctcaa ctgcgggaga aagcgactcc agaacagcca tgacccgttg
     5581 gctcatgctg tggtacagcg cataattgct ggaaaataca tgtattttct tctccaggcg
     5641 catctgtctc acctgaaacc agggctgccc cattctgatg ccaagggctt ttgcctccgg
     5701 gctgcgcgcg atcacacagc catcgttatt gctgagtacg atgaccggtt cgttgcgaag
     5761 gtccgggcgg aaaacttttt cacatgaggc gtagaaactg ttgatatcag ccagtgcaaa
     5821 cat
//
LOCUS       DAZOZD010000246         5726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOZD010000246 DAZOZD010000000
VERSION     DAZOZD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.249"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            10..999
                     /gene="ldhA"
                     /locus_tag="ACHUPB_003750"
     CDS             10..999
                     /gene="ldhA"
                     /locus_tag="ACHUPB_003750"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0760454.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1110..1532
                     /gene="hslJ"
                     /locus_tag="ACHUPB_003751"
     CDS             1110..1532
                     /gene="hslJ"
                     /locus_tag="ACHUPB_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0760455.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1529..1795)
                     /locus_tag="ACHUPB_003752"
     CDS             complement(1529..1795)
                     /locus_tag="ACHUPB_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0760456.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2069..5593
                     /gene="nifJ"
                     /locus_tag="ACHUPB_003753"
     CDS             2069..5593
                     /gene="nifJ"
                     /locus_tag="ACHUPB_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0760457.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1437 a         1480 c         1473 g         1336 t
ORIGIN      
        1 gaaagtctta tgaaactcgc cgtttatagc acaaaacagt acgacaagaa gtacctgcaa
       61 caggtgaacg agtcctttgg ctttgagctg gaattttttg actttctgct gacggaaaaa
      121 accgctaaaa ctgccaatgg ctgcgaagcg gtatgtattt tcgtaaacga tgacggcagc
      181 cgcccggtgc tggaagagct gaaaaagcac ggcgttaaat atatcgccct gcgctgtgcc
      241 ggtttcaata acgtcgacct tgacgcggca aaagaactgg ggctgaaagt agtccgtgtt
      301 ccagcctatg atccagaggc cgttgctgaa cacgccatcg gtatgatgat gacgctgaac
      361 cgccgtattc accgcgcgta tcagcgtacc cgtgacgcta acttctctct ggaaggtctg
      421 accggcttta ctatgtatgg caaaacggca ggcgttatcg gtaccggtaa aatcggtgtg
      481 gcgatgctgc gcattctgaa aggttttggt atgcgtctgc tggcgttcga tccgtatcca
      541 agtgcagcgg cgctggaact cggtgtggag tatgtcgatc tgccaaccct gttctctgaa
      601 tcagacgtta tctctctgca ctgcccgctg acaccggaaa actaccatct gttgaacgaa
      661 gccgccttcg atcagatgaa aaatggcgtg atgatcgtca ataccagtcg cggtgcattg
      721 attgattctc aggcagcaat tgaagcgctg aaaaatcaga aaattgggtc gttgggtatg
      781 gacgtctatg agaacgaacg cgatctattc tttgaagata aatccaacga cgtgatccag
      841 gatgacgtat tccgtcgcct gtctgcctgc cacaacgtgc tgtttaccgg gcaccaggca
      901 ttcctgacag cagaagctct gaccagtatt tctcagacta cgctgcaaaa cttaagcaat
      961 ctggaaaaag gcgaaacctg cccgaacgaa ctggtttaat cttgccgctc ccctgcattc
     1021 caggggagct tattcagata atccccaaag acctttcatc ttctattctt aaaatagccc
     1081 tgagtcagaa actgtaattg agaaccacaa tgaagaaagt agccgcgttt gttgcgctaa
     1141 gcctgctgat ggcgggatgt gtaagtaatg acaaaattgc tgttacgcca gaacagctac
     1201 agcatcatcg ctttgtgctg gaaagcgtaa acggtaagcc cgtgaccagc gataaaaatc
     1261 cgccagaaat cagctttggt gaaaaaatga tgatttccgg cagcatgtgt aaccgcttta
     1321 gcggtgaagg caaactgtct aatggtgaac tgacagccaa agggctggca atgacccgta
     1381 tgatgtgcgc taacccgcag cttaatgaac tcgataacac cattagcgaa atgctgaaag
     1441 aaggtgcaca agtggatctg accgcgaacc agttaacgct ggcgaccgcg aaacagacat
     1501 taacttataa gctggcggat ttaatgaatt aatagctgcc acagctcccg gcggcaagtg
     1561 actgttcgct acagcgtttg ccgttgggta atgcacacat cccaatcgcc gtaccatcca
     1621 gttgacgggc aacagaaagc gaaccgccga tcattgcaca atttgcttct ccactactgg
     1681 acatcgacgc ttttaaacct ggcgctacgt gcgccgcagt cgcctgctga acaggttcac
     1741 tactacacgc cgacaacaat aaagcggcac accctaccca aaacgctgct cgcatctctt
     1801 cttcctctga tcttcaagcc aaacgacacc gccataaata ataggcagca cagagggcgg
     1861 cgtcgagagc tgtcctgcgc gttgccccgc atttttactt ttttatggct atttttttgc
     1921 cctctgtttg atcaaaacat tcattacgct gatgtggggg acacaaaagc gaaaatgcag
     1981 aagaaagcca tttgctaaaa ttgaaggatt actactgggc gcgcagcaat ttcgtgcgcc
     2041 cctcatttgc gcaatgtaag ggtgtcatat gattactatt gatggtaatg gcgcggttgc
     2101 ttcggtggcg tttcgcacca gtgaagttat cgccatctac cctattaccc ccagttccac
     2161 gatggcagaa caggctgatg cctgggccgg aaacggcttg aagaacgttt ggggagacac
     2221 accatgcgtg gttgaaatgc agtcggaagc gggtgctatc gctaccgtgc atggcgcttt
     2281 gcagacgggt gccctttcaa catcgtttac gtcatcgcag ggtttgctgc tgatgatccc
     2341 gacactgtac aaactggcag gcgaactcac accgtttgtc ctgcatgtag cggcacgtac
     2401 cgttgccaca catgcactct ctatttttgg cgatcattcc gacgtcatgg cggtgcgcca
     2461 gacgggttgc gcgatgttgt gtgcggcaaa cgtccaggaa gcgcaagact ttgctctcat
     2521 ttcgcacatc gcgacgctga aaagccgcgt gccatttatt catttctttg atggtttccg
     2581 cacgtcccac gaaatcaaca aaattgtccc gctggccgat gacacgattc ttgatctcat
     2641 gccgcaggcc gaaattgatg ctcatcgcgc ccgggcgctc aacccggaac atccggtgat
     2701 ccgtggtacg tccgccaatc ctgacactta tttccagtct cgcgaagcca ccaacccctg
     2761 gtacaacgca gtctatgacc atgttgaaca ggcgatgaat gatttctctg ccgcgacagg
     2821 tcgtcagtat cagccgtttg aatattacgg gcatccgcaa gctgaacggg tgattatcct
     2881 gatgggctca gccattggca cctgtgaaga agtggttgat gaattgctaa cccgtggcga
     2941 aaaagtcggc gtgctgaaag ttcgcctgta ccgccccttc tcagccaaac acttgctaca
     3001 aactctaccg ggatccgtac gcagcgtggc ggtactggac agaaccaaag aacccggtgc
     3061 ccaggcagaa ccgctctatc tggatgtgat gaccgcactg gcagaagcct ttaataatgg
     3121 caagcgcgaa actctgcccc gtgtcattgg tgggcgctat ggtctttcat ccaaagaatt
     3181 tggcccggac tgtgtactgg cggtatttgc cgagctcaac gcggctaaac cgaaagcgcg
     3241 ctttacggtt ggtatttacg atgatgtaac caatctgtcc ctgccgttgc cggaaaacac
     3301 ccagccaaac tcggcgaaac ttgaagcctt gttttatggc cttggtagtg atggcagcgt
     3361 ttccgcgacc aaaaacaata tcaagattat cggtaattcc acgccgtggt acgcacaggg
     3421 ctattttgtt tacgactcca aaaaagcggg cggcctgacg gtttctcacc ttcgcgtgag
     3481 cgaacagccg attcgttccg cttatctcat ttcccaggct gattttgttg gctgccacca
     3541 gttgcagttt atcgataaat atcagatggc tgagcgttta aaacctggcg gcattttcct
     3601 gctcaacacg ccgtacagcg cagatgaagt gtggtcgcgc ttgccgcaag aagttcaggc
     3661 cgtgttaaac cagaaaaaag cgcgcttcta tgtgattaac gcggcgaaaa tcgcccgcaa
     3721 atgtggcctg gcggcccgta ttaataccgt catgcagatg gcttttttcc atctgacgca
     3781 aattctgcct ggcgatagcg ccctcgcaga attgcagggt gcgattgcca aaagttacag
     3841 tagcaaaggc caggatctgg tggaacgcaa ctggcaggct ctggcgctgg cgcgtgaatc
     3901 cgtagaagaa gttccgttgc aaccggtaaa tccgcacagc gccaatcgac cgccagtggt
     3961 ttccgatgcc gcccctgatt tcgtgaaaac cgtaaccgca gcgatgctcg cagggcttgg
     4021 tgatgccctc cccgtttcgg cgctgccgcc agacggcacc tggccgatgg gcaccactcg
     4081 ctgggaaaaa cgcaatatcg ccgaagagat ccccatctgg aaagaggaac tctgtaccca
     4141 atgtaaccac tgcgtcgcag cttgcccaca ctcagctatt cgcgcaaaag tggtaccacc
     4201 cgaagcgatg gaaaacgccc ctgccagcct gcattcgctg gatgtgaaat cgcgtgatat
     4261 gcgcgggcag aaatatgtct tgcaggtggc tccggaagat tgcaccggtt gtaacctgtg
     4321 cgtcgaagtt tgcccggcga aagaccgtca gaatccagag attaaagcca tcaatatgat
     4381 gtcgcgcctg gaacatgtcg aagaagagaa aatcaattac gatttcttcc tcaacctgcc
     4441 agaaatcgac cgtagcaaac tggaacgtat tgatattcgt acatcgcagc tgattacacc
     4501 gctgtttgaa tattccggtg cttgctccgg ttgtggcgag acgccgtata ttaaattgct
     4561 gactcagctc tatggcgacc ggatgttgat cgctaacgcc accggctgtt cttcaattta
     4621 tggcggtaac ctgccctcta caccgtatac caccgatgcc aacggtcgtg ggccggcatg
     4681 ggcgaactct ctatttgaag ataatgccga atttggcctt ggtttccgcc tgacggtcga
     4741 tcaacaccgt gtccgcgtgc tgcgtctgct ggatcaattt gccgataaaa tcccggcgga
     4801 attactgacg gcgttgaaat cagacgctac gccagaggtt cgtcgagaac aggtcgcagc
     4861 tttacgccag caactcaacg atgttgccga agcacatgaa ctgctacgtg atgcagatgc
     4921 actggtggaa aaatcaatct ggctgattgg tggtgatggc tgggcttacg atatcggctt
     4981 tggcggtctg gatcatgtat tgagtttgac ggaaaacgtc aacattctgg tgctggatac
     5041 gcaatgctat tccaacaccg gtggtcaggc gtcgaaagcg acaccgctgg gtgcagtaac
     5101 taaatttggc gagcacggca aacgtaaagc gcgtaaagat cttggcgtca gtatgatgat
     5161 gtacggtcat gtttatgtgg cgcagatttc tctcggcgcg cagctgaacc agacggtgaa
     5221 agcgattcag gaagcggaag cgtatccggg accatcgctg atcattgctt atagcccgtg
     5281 tgaagagcat ggttacgatc tggcactcag ccatgaccag atgcgccaac tcacagctac
     5341 cggcttctgg ccgctatatc gcttcgatcc tcgtcgtgcc gatgaaggca aactgccgct
     5401 ggcgctggat tcacgcccgc cgtcagaagc actggaagaa acgttacttc acgagcaacg
     5461 tttccgtcgt ctgaattcgc agcagccaga agtggcagaa cagttatgga aagatgctgc
     5521 agctgatttg caaaaacgct atgacttcct ggcacaaatg gccggaaaag cggaaaaaag
     5581 caacaccgat taaatgctct ggataaggat tacccaattc taaaaaaaag cccggacgac
     5641 tgttcgggct tgtcttttta tatgttggaa aatcagtggc aatgcaatgg cccaacagaa
     5701 aataaaatct gtgacaataa aggcat
//
LOCUS       DAZOZD010000247         5685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOZD010000247 DAZOZD010000000
VERSION     DAZOZD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.250"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            22..645
                     /gene="nfeR"
                     /locus_tag="ACHUPB_003754"
                     /gene_synonym="yqjI"
     CDS             22..645
                     /gene="nfeR"
                     /locus_tag="ACHUPB_003754"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0760458.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(752..2272)
                     /gene="aer"
                     /locus_tag="ACHUPB_003755"
     CDS             complement(752..2272)
                     /gene="aer"
                     /locus_tag="ACHUPB_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR0760459.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2690..4069
                     /gene="ygjG"
                     /locus_tag="ACHUPB_003756"
     CDS             2690..4069
                     /gene="ygjG"
                     /locus_tag="ACHUPB_003756"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0760460.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4111..4443)
                     /locus_tag="ACHUPB_003757"
     CDS             complement(4111..4443)
                     /locus_tag="ACHUPB_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR0760461.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4662..5645
                     /gene="ebgR"
                     /locus_tag="ACHUPB_003758"
     CDS             4662..5645
                     /gene="ebgR"
                     /locus_tag="ACHUPB_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0760462.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1382 a         1545 c         1434 g         1324 t
ORIGIN      
        1 atcaagcaaa aggaggctga tatgagccat catcacgaag ggtgttgtaa acatgaaggc
       61 cagccacgcc atgagggctg ctgcaaaggt gagaagtcag aacacgagca ctgcggacac
      121 ggtcaccagc atgaacacgg tcaatgctgc ggtggtcgcc acagtcgcgg cggcggtcgt
      181 cggcaacgtt tctttggtca cggtgaattg cgtctggtga ttctggatat tctctcgcgc
      241 gatgacagcc acggttacga attgattaaa gcgattgaga atctaaccca ggggaattac
      301 accccaagcc cgggcgtcat ctacccaacg ctggattttc tgcaggagca gtcgctgatt
      361 accatccgcg aagaggaagg tggtaagaag cagattgcgc tgaccgaaca aggcgcgcag
      421 tggctggaag aaaaccgcga acaggtggag atgattgaag aacgcatcaa agcgcgttgc
      481 gttggtgctg cgctgcgcca gaacccgcaa atgaagcggg cgctggataa ttttaaagcg
      541 gtgctggatt tacgcgtcaa ccagagcgat atcactgatg cacaaataaa aaagatcatt
      601 gcggtgatcg accacgccgc ttttgatatt acccaactgg attaatcacc gattgccgga
      661 tgcggcgtac cctacacacc ccgcaggcct gataagatgc gccagcatcg catcaggcat
      721 tgtgctccaa ccgccggatc cggcataccg attaatgcag caccgtcacc gcgtcttcca
      781 gtcggctggc gcggtgtttc accatcgccg acacctgcgc actctcttcc accagctcag
      841 catttttctg ggtgatcagg ttaagctcat ccactgcacg ggtcaggctg gaaagcccat
      901 cggcctgttc cagcgttgaa tggctaatct gggcgatcaa ctgggtgacg tttttcacct
      961 gtgccacaat atcttccatc gtccgtccgg cggcgtgtac ctgctgcgaa ccggattgca
     1021 ccttatcagc actggcatca atcagcttgc gaatatcgtt ggcagcatta gcgctgcggc
     1081 tggctaaatg acgcacttcc cctgccacca ctgcaaaacc tttgccctgt tcgccggcac
     1141 gcgccgcttc caccgccgca ttcagggcca gaatattggt ctgaaacgca atatcgttaa
     1201 tcagcgaagt aatggtgcca atgcgctggg tactgtcggc gatatcgtcc attgtcttga
     1261 tcaccgtggt catcgcctcc ccaccctgca ctgccgcatt actggcggtg atagaaagtt
     1321 tatcggcagc cgacgccgtg gcagagttct gtttcaccga cgccgccatt tggttcatgg
     1381 tcgccaccgt ttgctgaacg ttatcaactg tctgctgggt atgttcgttc agttcatcgg
     1441 tgcctttcgc cagcgtctca ctgccatttc tgacactgga cacctggctt gagacatcgt
     1501 taattagcca acggcacatc aggccaagct gccctaccgc gcgtaatgtc agccccagct
     1561 catcgctgcg attcaggtgc tcaacactat tacgctctcc ggtcgccacc ttcagtgcct
     1621 gacgggcgac attttctatc gggcgcacaa tctgccattc aaaacaagcg cttgccaaca
     1681 ataccactaa cacacagagg aaatacgtca ccaccggggc agcaacaaac caaagcatgg
     1741 ccgccagcaa gataaacatc agggtcatca ctccacgcgc ccgccagcga agcggtaatg
     1801 aaggcagttt acccagccag cctttacgca ccaccaggcc tttatgaata cgcttactgg
     1861 tacgtccggc gttcagcgct ttgtacagcg gctccaccgc cgcaatctct tcatccgtcg
     1921 cccgggtacg aatcgacata tagccactga tttttccctc gcgcaccatc ggtaccgcat
     1981 tggcccgcac ccaataatgg tcgccatttt tgcggcgatt tttcacgatg ccgctccagg
     2041 gctccccttt tttcagggtg aaccacatat ccgcaaacgc cgcttttggc atatccgggt
     2101 gacgcaccat gttgtgcggc tgcccttgta actcttgcaa ggtaaagccg ctcacctgca
     2161 caaaagtgtc attagcatga gtgatatagc tttgcagatc ggtagtggac atcagagtgg
     2221 tatcgtccgc cagcggggta ttttgctggg tgacatacgg atgagaagac atgttcgcgt
     2281 cctgtgcagg ttatatggtt gttaacttct tgtcagagtt tatgtcggcc ccgctgcggt
     2341 tatctttaac cgattaattt gatttagatc gcaatttgcg attaaagcac aaatctaatt
     2401 ctttgattta aaatactttc actctgttac tatacgaaaa cgttaattat cttgcccaaa
     2461 aatcaggcaa ttattgccct gaaaacgtgc atttgcgcag caatcatcaa atccataccc
     2521 gacaaaaacc gtgcaaaata acaacaaatg ttaacagata gcattaaata ttgcacaaat
     2581 gataaccgaa tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct
     2641 gcaatactta aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt
     2701 accttcgagc gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg
     2761 aacgctggat catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga
     2821 gcatgtcaat ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg
     2881 agccgtagag tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt
     2941 tatcgactgc ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt
     3001 ttccgccgta cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc
     3061 gttacgggcg atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag
     3121 cttcttctgt aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta
     3181 ccagtcaccg cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc
     3241 acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgcagttact
     3301 gccgggcttc cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga
     3361 gtgcaaaaaa accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg
     3421 tggcgtaatt ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt
     3481 cggcgcactg atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt
     3541 cgcctgcgag catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg
     3601 cggcgtgatg ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga
     3661 caacccattc ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct
     3721 ggcgaccatc aatgtgttgc tggagcagaa cttaccggct caggctgatc aaaaaggcga
     3781 tatgttgctg gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc
     3841 gcgtggtaaa gggatgttga tggcgattga gtttgttgat aacgaaatcg gctataactt
     3901 tgccagcgag atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac
     3961 gatccgcatt gaaccgccac tgacactgac cattgaacag tgtgaactgg tgatcaaagc
     4021 ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg
     4081 atggcgatac gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat
     4141 ccgctccggc gttaacagca cgctttcact gccgtcatcg gtttccgcac acaacagcat
     4201 gcattccgac gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt
     4261 ttttcccatc agctcctctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt
     4321 tttttccccc acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac
     4381 ttccacaatc tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc
     4441 cattgcttcc tcccttcaag taaaatttta ctaaactata gcaaaatttt ttctcaatcc
     4501 tgtaggctaa aaatggagaa tgcaggcgtg atcacattcg taagccgctg tgttaccgtt
     4561 acagcgtcaa agaaacgcgc tttatttact gaaaacaggt gacccgataa gcacttcctc
     4621 tacaatgggg gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ttaaaagaca
     4681 tcgcaatcga agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga
     4741 cattgaatgt gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt
     4801 acaagaccag tagcgcccgt aaactacaga caggcgcagt caaccaacac catattctgg
     4861 ctatctacag ctaccagcag gagctggaga tcaacgatcc ttactatctg gcgatccgcc
     4921 acggcattga aactcagtgc gaaaagctgg cgatcgaact gaccaactgt tatgaacaca
     4981 acggattacc agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg
     5041 ccctgcgcgc cgctgccagc gcgttgaccg acaatatctg ttttatcgac tttcacgaac
     5101 ccggcagcgg ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg
     5161 acttctatat caaccagggc gttaatcgta ttggttttat tggcggtgaa gatgagcctg
     5221 gcaaggcgga tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac
     5281 gcgaagagga tatctggcgc ggcggttttt ccagttcgtc gggttatgaa ctggcaaaac
     5341 aaatgctggc gcgggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta
     5401 tcggcgtact gcgggcaatt catgaacgtg gcctgaacat cccacaggat atttcgcttt
     5461 tcagcgttaa cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca
     5521 tccattccga gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg
     5581 gtcgcgcgct gccgctgcta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc
     5641 gttaaatccc cttacacact gtccggcaat catttttgcc ggaca
//
LOCUS       DAZOZD010000248         5683 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOZD010000248 DAZOZD010000000
VERSION     DAZOZD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.251"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(266..660)
                     /locus_tag="ACHUPB_003759"
                     /pseudo
     CDS             complement(266..660)
                     /locus_tag="ACHUPB_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            603..1109
                     /locus_tag="ACHUPB_003760"
     CDS             603..1109
                     /locus_tag="ACHUPB_003760"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0760463.1"
                     /translation="MLEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHA
                     DSYGYRPGKSAHDALKQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWV
                     ILYCRRWMEAPMQSCENGELITRTRGTPQGGVISPLLANLFHHDAFDLWMEREYRGYR
                     LRGTLTIL"
     gene            1124..1651
                     /locus_tag="ACHUPB_003761"
     CDS             1124..1651
                     /locus_tag="ACHUPB_003761"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFR0760464.1"
                     /translation="MSDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSF
                     TFLGYDFKVRTLKNFKGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARR
                     YNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEY
                     PKLFVHWYLLRASNE"
     gene            1734..1913
                     /locus_tag="ACHUPB_003762"
                     /pseudo
     CDS             1734..1913
                     /locus_tag="ACHUPB_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1940..2425)
                     /locus_tag="ACHUPB_003763"
     CDS             complement(1940..2425)
                     /locus_tag="ACHUPB_003763"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0760465.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAADLRQ
                     K"
     gene            complement(2681..3661)
                     /locus_tag="ACHUPB_003764"
     CDS             complement(2681..3661)
                     /locus_tag="ACHUPB_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR0760466.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3661..4860)
                     /locus_tag="ACHUPB_003765"
     CDS             complement(3661..4860)
                     /locus_tag="ACHUPB_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0760467.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5034..5168)
                     /locus_tag="ACHUPB_003766"
                     /pseudo
     CDS             complement(5034..5168)
                     /locus_tag="ACHUPB_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5182..5535)
                     /locus_tag="ACHUPB_003767"
                     /pseudo
     CDS             complement(5182..5535)
                     /locus_tag="ACHUPB_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5602..>5683)
                     /locus_tag="ACHUPB_003768"
     CDS             complement(5602..>5683)
                     /locus_tag="ACHUPB_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760468.1"
                     /translation="FVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1569 a         1296 c         1333 g         1485 t
ORIGIN      
        1 agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag gaatgaggtg
       61 gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga agctgaacga
      121 aagtaaactg tccgatgaag catcgaaatc ttagatgatg tcaaaatggg gccgtacagc
      181 cgccccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg
      241 cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc
      301 gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg
      361 aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga
      421 tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc
      481 gtcgcaacaa gggagccccc ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac
      541 gtgatggcaa tctatacaag atctggaacc ggctatgttc gggaacatgg tttccgccac
      601 cagtgctgga aaaacggatc ccgaagtcga atggcaagga acgcattctg gggatcccaa
      661 cagtatccga tcgaattgct cagggagcga taaaactttt catggaagaa aagcttgacc
      721 cgattttcca tgctgattca tatggttacc gaccaggcaa atcagctcat gacgctctga
      781 aacaatgtgc catccggtgc tggcgttata gctggatact ggaagtggac atcagtgcgt
      841 ttttcgacca tgtgagacat gatctggtgc tcaaagctct ggaacaccac gggatgccta
      901 aatgggtcat cctgtactgt cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg
      961 gagaattaat aacccgaaca cgaggcactc cgcagggcgg ggtcattagt ccgctacttg
     1021 ctaacttgtt ccatcacgat gcatttgatt tgtggatgga aagagaatat cgggggtacc
     1081 gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg atgcaacaag
     1141 gctgaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga atgcagggaa
     1201 aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga gtttcacctt
     1261 tctcggatat gacttcaaag tgcgtacgct gaagaatttc aaaggcgaac ggtaccgaaa
     1321 atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa tcaagaagtg
     1381 gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct acaatgcgat
     1441 agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca actatcgact
     1501 gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca gactttcgaa
     1561 ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc tatttgttca
     1621 ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg agagtgtcac
     1681 gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga ctcgcgggtt
     1741 ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca tgccgtctga
     1801 acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag gactggctgg
     1861 caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag taaatatcaa
     1921 tacggttctg acgagccgct tacttttggc gtaaatctgc tgcgccttta ctgaacacaa
     1981 tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa
     2041 ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt tttattggta
     2101 cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg ttataatcat
     2161 ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt acatcatggt
     2221 cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc atcgcaacag
     2281 tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt ttggtctttc
     2341 ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta gccagatcaa
     2401 tgccggtgat tttctgttca gacatggtat tccgcttact tttgtggtta aagtttatcc
     2461 atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat cggttactgt
     2521 taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa caggcgattt
     2581 caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt taaaacagcc
     2641 aattaaaaac tggtgagttg ctggcgaaaa acttgaattt tcacagcgaa ttcagattat
     2701 cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac ggtaatctgc
     2761 cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta tctttactat
     2821 caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca agcaatgaag
     2881 acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca gaaagattgt
     2941 catcagcagt aataagctca atactcggcg agatattctt cacaacttca tcaatagata
     3001 tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg gcgtatgtca
     3061 gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct gcttggaggg
     3121 cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc tggttcatac
     3181 cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt tcaagtgacg
     3241 tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca tccgttacaa
     3301 gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa ccatccaaaa
     3361 tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt aaatgtatag
     3421 ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg ccattcacct
     3481 cttcaaccac aaaagtttga gtgtccactt tgtcatgatc aaccttgatc tcagtaaatt
     3541 ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt tcttcagtat
     3601 tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt ctgttttcca
     3661 tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga aatcttctgc
     3721 tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag gattcgcaga
     3781 tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg gcattgaaac
     3841 atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt tatgatctga
     3901 cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg aacatccatc
     3961 ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg tggaatggaa
     4021 atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa tggcattttt
     4081 caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat catgcttcag
     4141 cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata gatgctcctc
     4201 gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg gcataacata
     4261 aacgccaggg ataactgacg gaacgataaa atcttccagt agttcctcac gactcacgtt
     4321 ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca agtagttcag
     4381 aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta aatcttctga
     4441 cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca cggttttaga
     4501 cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac gatcgcgata
     4561 tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta atgcatattt
     4621 tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca tctcaccgac
     4681 tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag actttgaata
     4741 gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct gttttgttag
     4801 ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa aactagtcat
     4861 tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat tagcagggta
     4921 atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg ctgatttatt
     4981 aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa gacgctgatt
     5041 tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc aggctccaga
     5101 tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga ctgtcgcaac
     5161 aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg agccgatgac
     5221 tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat
     5281 gcgggtgcgg agggtccggt tgttacgttc aatgcgttgc gtgaagattt tgccggtcag
     5341 gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg tcaccatgcc
     5401 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     5461 cgtggcccga aagtcgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     5521 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     5581 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     5641 tttctgtctg agtcgatagc tttctccttt gatttgaacg aaa
//
LOCUS       DAZOZD010000249         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOZD010000249 DAZOZD010000000
VERSION     DAZOZD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.252"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUPB_003769"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUPB_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0760469.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUPB_003770"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUPB_003770"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0760470.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUPB_003771"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUPB_003771"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0760471.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUPB_003772"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUPB_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0760472.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZOZD010000250         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOZD010000250 DAZOZD010000000
VERSION     DAZOZD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.253"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHUPB_003773"
     CDS             complement(<1..513)
                     /locus_tag="ACHUPB_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0760473.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHUPB_003774"
     CDS             complement(759..1211)
                     /locus_tag="ACHUPB_003774"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0760474.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUPB_003775"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUPB_003775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0760475.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUPB_003776"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUPB_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0760476.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHUPB_003777"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHUPB_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUPB_003778"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUPB_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0760477.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHUPB_003779"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHUPB_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0760478.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZOZD010000251         5618 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOZD010000251 DAZOZD010000000
VERSION     DAZOZD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.254"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUPB_003780"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUPB_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0760479.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHUPB_003781"
     CDS             1598..4654
                     /locus_tag="ACHUPB_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0760480.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHUPB_003782"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHUPB_003782"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0760481.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHUPB_003783"
     CDS             5161..5349
                     /locus_tag="ACHUPB_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0760482.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
BASE COUNT         1386 a         1439 c         1496 g         1297 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctg
//
LOCUS       DAZOZD010000252         5614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOZD010000252 DAZOZD010000000
VERSION     DAZOZD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.255"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..586)
                     /gene="araH"
                     /locus_tag="ACHUPB_003784"
     CDS             complement(<1..586)
                     /gene="araH"
                     /locus_tag="ACHUPB_003784"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0760483.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
     gene            complement(601..2115)
                     /gene="araG"
                     /locus_tag="ACHUPB_003785"
     CDS             complement(601..2115)
                     /gene="araG"
                     /locus_tag="ACHUPB_003785"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0760484.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(2185..3174)
                     /gene="araF"
                     /locus_tag="ACHUPB_003786"
     CDS             complement(2185..3174)
                     /gene="araF"
                     /locus_tag="ACHUPB_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR0760485.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            3971..4474
                     /locus_tag="ACHUPB_003787"
     CDS             3971..4474
                     /locus_tag="ACHUPB_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0760486.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4553..4804)
                     /gene="yecJ"
                     /locus_tag="ACHUPB_003788"
     CDS             complement(4553..4804)
                     /gene="yecJ"
                     /locus_tag="ACHUPB_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0760487.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="ACHUPB_003789"
     CDS             complement(4919..5005)
                     /gene="azuC"
                     /locus_tag="ACHUPB_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0760488.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5268..5591
                     /locus_tag="ACHUPB_003790"
     CDS             5268..5591
                     /locus_tag="ACHUPB_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0760489.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         1464 a         1432 c         1257 g         1461 t
ORIGIN      
        1 aggtggtttt attcagcaac aaaccaaaga taatcagaca cgcgacggtg agccagattg
       61 gcgcaggcag accgaaccag ttagcgtaac caagggcaaa gaagctttca tcttcgatac
      121 cgaccgcttt accgtctgaa atgatgtacg ccagacctcg aacaatctgc atcgttgcca
      181 gtgttgtgat cagagcattt attttcagtt tggcgataac aaagccattg accaggccac
      241 agagaatgcc aagcagcaac cccgctgcca cgccaatcca caggctttca gtcaggttga
      301 taaccaccgc cgtggtgaca cccgcacagg caattacgga ggcgacagaa aggtcaaagt
      361 caccggaagc aaggcagaac aacatgccac acgccaccat ccccgacatg gaaattgcca
      421 ggcccaaccc tttcatatta atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga
      481 taaagagcac cgcaaacacc accagcatgc cgtactgatc ccagatacgc ccgaagctga
      541 atgacgactt aggtgcgcca gaacccgatg tagaaacaga agacatcata ctctccttac
      601 tcaggcaact gcctggctga ctttaggcat cgcaaggctc agtgcctgac gctcatctgc
      661 ctgctcgtgt aacaattcac cggcgatttc accttcccgc atcaccacaa tccggtcggc
      721 aacgccgagg acttcaggta agtcgctgga ggcaaacagc accgccacac cctgcgccgc
      781 cagcgcataa attacgttat atatttcgtg cttagcacca acatcaatgc tgcgcgtagg
      841 ttcatccagc aaaatgacct tcatctcttc cgataaccag cggcccagaa tggctttttg
      901 ctgatttccg cctgagagat tcatgatcag ttgctcagcg cccggcgttt tgatgttgag
      961 cgaacgaatg tgctgatcgg cattgttttc ttcccaaccg ttgttgatta cacaaccgcc
     1021 gagcacatgt ttgcgtctgg cactgatgtt gatattgtcg cgaacggagt gcacgggaat
     1081 aatgccttcc gctttgcgat cttccgggca gagcatcatg cctgcggcaa tggcgtggct
     1141 cggtttacga atatcgatcg gctgttggtc gatataaacc tgaccggcgg tgatttgcgt
     1201 cccgccaaac aagcctttca ttaattcgct acgccccgcc cctaccagac caaacagccc
     1261 aacaatttca ccactgcgaa ccgccagact tattggcgta cgcacgcctg gtgctttcac
     1321 agcatcaaga cgcaggcgct cctcgccata actacgaggt tgccagccgt agatatcgcc
     1381 aatgtcgcgc ccgaccatcg cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt
     1441 aaaggttttg acataacgtc catctttaaa gacagtgatg gcatcgctga gggcaaatat
     1501 ttcttccata cggtgagaaa cgtataagat cacccgcccc tcttttcgca gttcacgaat
     1561 aacgcggaaa agattgtcga tttcgcgggc agaaagggag ctggttggct catcaaaggc
     1621 gataatttta gcgttacgcg ccagcgcctt ggcgatttca accatctgcc actgaccaat
     1681 ggagagatat ttcagcggcg tgtccgggtc aatatccata ccaagatgtt taagttgtaa
     1741 acccgcctca taattcagca atgagcgatt cacaatgccg cctttatgcg gcagctggcc
     1801 gagatagatg ttttccgcga cggtcatttc cggcacgaga tgcagttcct ggtaaataat
     1861 cgccacaccc gcgttaagtg ctgcggtcgt gtcggaaaag gacatttcct gcccattaat
     1921 cactacagaa cccgtggttg gcgcatagtt gccgctgagg atttttaaga gagttgattt
     1981 tcctgcgcca ttttcaccca tcaacgcatg aacctgaccg gcatagcagt caaaactaat
     2041 atccgtcagc gccttaacgc cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg
     2101 ggtagactgt tgcataacgt ctccgtgaat cactggtcgt tcacaccccc tctgcatcat
     2161 gcagagggga atttttccgg caaattactt accgcctaaa ccttttttct ccagttcttc
     2221 tttaaagttg tcacgcgtga tcagcaccac gtcggtaact tcggtaaatt ttggcggttc
     2281 aacgtctttt gctacccagt tgtaaagcat ttcgctggat ttatagccat gtacgtccgg
     2341 gcttggcagc agggaaccgt agaagccggt tgcctgtgct ttagacagtt cgctcaacgc
     2401 atccacaccg ttaatgccaa tgccgatgat atcggcagct ttaaagccct gaccttccgt
     2461 cgcgcgtacg ccgcccagca cggtgctgtc gttcataccg acgatcagcc aatgtttaac
     2521 ttccggatgt tgaaccagca ttgagttggc agcgtcaaat gcccccggga tgtcgttaga
     2581 tttggtaggt acctgataaa tttgtttttc cgggaatccg gccgctttca gcgcatccat
     2641 agaacccgtt gtacggcgac gtgccgtatc cagttcgttg gcggtaatcg ccatcaccgc
     2701 gctttctttg acatcccagc cacgtttctg catctcttta tacagttcct gaccctgacg
     2761 ttcgccaatt ttagtcgccg ccatcatcac cagcggaacg gtatccattg gcttaccttt
     2821 ggcgttaaca aactggtcat ccacggcaat gactttcata tcgtagccac gcgctttcgc
     2881 gacgatggca gagccgagtt tggggtccgg agtacaaata acgaaacctt ttgcgccact
     2941 ggcagccagg ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat
     3001 aacctcaaac cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca
     3061 cggctcttcc ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg
     3121 tgacataacg gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct
     3181 ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat gactttcgtt aaattaatcg
     3241 gaaaacaaag cattaccttt tatctaaaag ataagtgacc gtgttgacat agttttagcg
     3301 agaaattaat tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca
     3361 taaatttagc gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca
     3421 cagcagatta atccataaga ttagcctgga aatccttgtt gtctttggta cccatgcggg
     3481 atgtcttctt tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt
     3541 gtaataaccg caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc
     3601 actgtgctca ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa
     3661 cgttttataa aatcatcact tcatcatgaa ttcaaattca ttgattaata tcaacaagat
     3721 acaaaaagca ctatcattaa aattcattgc agttacattg atttcatcaa tgaaatgtaa
     3781 aaatatataa acttgatgat ttaagcattt tcttataccc gttcagacgt tattcttatt
     3841 tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa aggaattatt
     3901 ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc tggatataag
     3961 gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa tgaaccgcga
     4021 gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac agagtctgaa
     4081 cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat
     4141 gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg atgttcccgg
     4201 tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat acgagcaacg
     4261 ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg attcaaccgt
     4321 caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat
     4381 tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca
     4441 tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa tgcattgcgc
     4501 cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt ttttaatggg
     4561 attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg attgcccgca
     4621 tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc agctgactgg
     4681 acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga atttgtttga
     4741 taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc agcggctggg
     4801 acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc acatcttcag
     4861 cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag gttttttgtt
     4921 atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac
     4981 ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata caggtgttat
     5041 tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatgt gatacaaatc
     5101 acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc cctatttctg
     5161 acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc
     5221 gctgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg agacttctga
     5281 ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa gcccatgtga
     5341 gcgaagttga cgcggcaaca ggtattgtac ggttggttta tgaccaggct tttttgcagc
     5401 atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag
     5461 gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt
     5521 ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc gccttccctg
     5581 tctttctcta aatattttaa tcagcgaggg gatc
//
LOCUS       DAZOZD010000253         5609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOZD010000253 DAZOZD010000000
VERSION     DAZOZD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.256"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUPB_003791"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUPB_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUPB_003792"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUPB_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR0760490.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUPB_003793"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUPB_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0760491.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUPB_003794"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUPB_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0760492.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUPB_003795"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUPB_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0760493.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUPB_003796"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUPB_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0760494.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUPB_003797"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHUPB_003797"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0760495.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="ACHUPB_003798"
     CDS             complement(5024..5239)
                     /locus_tag="ACHUPB_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0760496.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1255 a         1623 c         1369 g         1362 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tcccgccccc cgacagacct gtgctatcgg aaagaaaaaa
     5281 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
     5341 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
     5401 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
     5461 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggggcatt tcattatttt
     5521 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
     5581 catgattcca gtccatgcaa aatctgatt
//
LOCUS       DAZOZD010000254         5589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOZD010000254 DAZOZD010000000
VERSION     DAZOZD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.257"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..725
                     /gene="yneK"
                     /locus_tag="ACHUPB_003799"
     CDS             <1..725
                     /gene="yneK"
                     /locus_tag="ACHUPB_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0760497.1"
                     /translation="PKPVTMGRVFQTGVERVLFLFLNDFIEQFPMFNPGVPIKIAHTP
                     HIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLITA
                     ILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQESPHFQDLFLFFANML
                     LHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKNETPPKVTHEDLVKNR
                     PRSPVRPPIPATSKTPDLPERH"
     gene            875..2065
                     /gene="ydeA"
                     /locus_tag="ACHUPB_003800"
     CDS             875..2065
                     /gene="ydeA"
                     /locus_tag="ACHUPB_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0760498.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2090..2755)
                     /gene="marC"
                     /locus_tag="ACHUPB_003801"
     CDS             complement(2090..2755)
                     /gene="marC"
                     /locus_tag="ACHUPB_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0760499.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2967..3401
                     /gene="marR"
                     /locus_tag="ACHUPB_003802"
     CDS             2967..3401
                     /gene="marR"
                     /locus_tag="ACHUPB_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0760500.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3421..3804
                     /gene="marA"
                     /locus_tag="ACHUPB_003803"
     CDS             3421..3804
                     /gene="marA"
                     /locus_tag="ACHUPB_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0760501.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3836..4054
                     /gene="marB"
                     /locus_tag="ACHUPB_003804"
     CDS             3836..4054
                     /gene="marB"
                     /locus_tag="ACHUPB_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0760502.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4111..5550)
                     /locus_tag="ACHUPB_003805"
     CDS             complement(4111..5550)
                     /locus_tag="ACHUPB_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0760503.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         1457 a         1387 c         1242 g         1503 t
ORIGIN      
        1 acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc
       61 tgtttctgaa tgattttatt gaacaatttc caatgttcaa ccctggcgtt ccgatcaaaa
      121 tagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct gcagattact
      181 tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc
      241 cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga
      301 tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga
      361 tggattcatt aggttctaac aaacatgtac tacccgaagt ctgcatctgc tttttattcc
      421 ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt
      481 tattgcacta tcaccaattt atgaatccca atgaaagtga tttgaatgac gtgttgatgc
      541 cagcatcatt aagtgatgat aaaattatca agcatatggc gcgcaggacc ctcgaactgt
      601 ttgtaaaaaa tgaaacaccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc
      661 gctcccctgt cagaccacct atacccgcaa cctccaaaac gccagacctc cctgaacgtc
      721 attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca gagaaacgga
      781 aaacgcgatc cagatcacaa atacattgta ttcacatcat taaccgtttt aagatcattt
      841 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
      901 agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga
      961 atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca
     1021 ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat
     1081 gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat
     1141 tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat
     1201 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
     1261 ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc
     1321 gatggtctta ggtttacctc tcgggcgcat tgtggggcag tatttcggtt ggcgaatgac
     1381 cttcttcgcg attggtattg gggcgcttat cacccttttg tgcctgatta agttacttcc
     1441 cttactgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
     1501 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
     1561 cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc
     1621 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
     1681 taatcagtat gcgtctgcgt tggtgagtac ggcgattgcg ctgttgctgg tgtgtctggc
     1741 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
     1801 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
     1861 tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg
     1921 tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg
     1981 cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc gctggccagt
     2041 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg
     2101 ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga
     2161 atcccatcag acgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc
     2221 cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
     2281 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa
     2341 taatcatcgc tatggttccc ggaccagcgg tacttggcat tgctagcggc acaaaggcaa
     2401 tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa
     2461 tcgctttctg ttgcggaaag agcatccgaa aaccgataaa cgcgacgatt aagccgcctg
     2521 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccggcgtaat
     2581 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
     2641 cggcactgtt catgttgcct gccaggccaa gaaatagcgc gacagttgtt aatgggttag
     2701 ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg
     2761 gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag
     2821 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
     2881 gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt atcctctgca
     2941 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
     3001 ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt
     3061 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
     3121 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga
     3181 cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg
     3241 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc
     3301 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
     3361 ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg
     3421 atgtccagac gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac
     3481 aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatgg
     3541 cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata catccgcagc
     3601 cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat actctatctg
     3661 gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa aaattacttt
     3721 gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg ctttttacat
     3781 ccattaaatc attacaatag ctagttgata acgtgacaac gtcactgagg caatcatgaa
     3841 accactttca tccgcaatag cagctgcgct tattctcttt tccgcgcagg gcgttgcgga
     3901 acaaaccacg cagccagttg ttacttcttg tgccaatgtc gtggttgttc ccccatcgca
     3961 ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt cggatgcgct
     4021 cggcgtgccc tattataatc aacacgctat gtagttattg tcaggctctg atcacagagc
     4081 ctgacatctc cgtaatatct tcctcaagtc tcaaatattt tcgccattgt tggcgatgag
     4141 atcccgatac cagtaaaacg acgctttacg tatccgttcc agcgttccct tcccttcgtt
     4201 gtctttatcg acataaatca ttccgtagcg ctttttcatt tcccctgttc cggcagaaac
     4261 caggtcaatg cagccccacg gggtgtagcc aattaagtca acaccatctt caactacggc
     4321 ttttttcatt tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat cgttcaccgt
     4381 gccgtcagct tgtctctggt caaccgcacc aaatccattt tcgacaataa acagcggcaa
     4441 ctggaaatga tcccagaacc agttgagtga ataacgtagc cctgccggat caatttgcca
     4501 tccccattcg gatgtatcga tataagggtt gctgaccaaa tctcgtggtt caacataatc
     4561 cagttgcggg ttatcgggag aaaattgtgt cgtaaaagac atgtagtagc taaaaccgat
     4621 aaagtcgaca catccactgg caagaatcgc gttatcttct ggtgtgatat cgaggttgaa
     4681 tcctttcctg gcaaagtaat tcagcatatg ttgcggataa tatccacgag catgaacatc
     4741 agtaaaccag taacgacgat gcatcgcttt cgtggccatc atcatatcgt tgggtgcaca
     4801 cgtcagagga tagatggggc acatggcgat catacagcca atgttaaatt caggattgat
     4861 cgattttcca gtctgtaccg ccagggcact ggcaactaac tcgtaatgta ccgcctggta
     4921 cattatttgt tcgcgctcat tgatatcttc ctctggcgaa tacaagatac cggaattagt
     4981 aaatggacac aggctttcgc tgaaattcac ctgattattg atttcgttaa acgtcatcca
     5041 gtactttact ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga taaaaaagtc
     5101 gatcagctta cggtttcgcc agccaccata ttttgtcacc agatgataag gcatctcaaa
     5161 atgcgaaagc gtcaccacag gttccattcc ttgcttcagg cattcatcga acagatcatc
     5221 ataaaattgt aaaccctctt cattcggctc ctgttcgtca ccttgcggaa agattcgtgt
     5281 ccaggcaatg gaagttcgaa agcatttgaa tcccatctcg gcaaataact gaatatctgt
     5341 tttatagcga tgataaaaat caattgcttc atgattggga taattaagcc cgtcgataac
     5401 gccttctgtc acttcacgcg gcaccccgtg agcgccagca gtcatcacat cagcgatact
     5461 gatgcctttt cctccttcat tccagccacc ttccaactga tgcgcggcta ccgcgccacc
     5521 ccataaaaaa ccttttttaa atcctgacat acacaactcc ttaactaaaa tagattacca
     5581 ccagacttc
//
LOCUS       DAZOZD010000255         5577 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOZD010000255 DAZOZD010000000
VERSION     DAZOZD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.258"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(124..843)
                     /gene="dcuR"
                     /locus_tag="ACHUPB_003806"
     CDS             complement(124..843)
                     /gene="dcuR"
                     /locus_tag="ACHUPB_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0760504.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(840..2471)
                     /locus_tag="ACHUPB_003807"
     CDS             complement(840..2471)
                     /locus_tag="ACHUPB_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0760505.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2652..2882
                     /gene="yjdI"
                     /locus_tag="ACHUPB_003808"
     CDS             2652..2882
                     /gene="yjdI"
                     /locus_tag="ACHUPB_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0760506.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2894..3166
                     /locus_tag="ACHUPB_003809"
     CDS             2894..3166
                     /locus_tag="ACHUPB_003809"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0760507.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3393..3689
                     /gene="ghoS"
                     /locus_tag="ACHUPB_003810"
     CDS             3393..3689
                     /gene="ghoS"
                     /locus_tag="ACHUPB_003810"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0760508.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3717..3890
                     /gene="ghoT"
                     /locus_tag="ACHUPB_003811"
     CDS             3717..3890
                     /gene="ghoT"
                     /locus_tag="ACHUPB_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR0760509.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4009..5526)
                     /gene="lysS"
                     /locus_tag="ACHUPB_003812"
     CDS             complement(4009..5526)
                     /gene="lysS"
                     /locus_tag="ACHUPB_003812"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0760510.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
BASE COUNT         1456 a         1272 c         1321 g         1528 t
ORIGIN      
        1 agcgatgatg cagggataat tatatttgat ctggcacaag ttttactgat gaaggatgta
       61 acttgtgcca ggggtatttt gcattacggt aataattatt acttacagat aaccagcgat
      121 ccgttattgg caatattgtt tcagtagtga gtagtgttct gcctgaatac ggtaacggta
      181 aactggacgc cccgtgacgc cataatggat actggtgaac aagatgtggc agttgaccag
      241 ccagatgagg tatttacggc aggaaacacg cgaaatgtta acctcgttgg ctagctcgtc
      301 ggttgaaaat tcatagtcct gatgcgcgtc aatccactgg cacagtgtgc gtaacgtctg
      361 cggcgttaag ccttttggca agcgacgagg atcctgttcg ttggagctgc tgccgtggat
      421 tagctgatca agctcggcct ggtcataata ctgatgtttt tccagcgcca ttttcttttg
      481 ccgccagtcg gtgagcgcct cttcaaagcg ggaagcctgg aagggtttga tcaggtaatc
      541 cacgacaccg taatgcagcg aatctttaat ggttgccgca tcggctgcgg aggagatgac
      601 aatcacatca cttttgcaac gcgcgttatg caggacaggc agtaaatcga gcccgttctc
      661 tttttgcata tagatatcga gcaatatcag gtcgataggc gtatcgctat tgaagataat
      721 ctctttggct ttctccagcg tcgaggctgt tccacagcat tgaaagcctg ggatttgtgc
      781 tacgtatcgg cgattcagct ccgcgaccat tgcgtcgtca tcgataatta atacattgat
      841 catctgttcg acctctcccc gtcccagggt atctggacaa aaaattgtgt gaaaatcccg
      901 ggttccgatt ccacggcgat gctgccgccg agattttcta cctgttgttt gacaagtgct
      961 aaaccgacgc ctcgctcgct tccttttgtc gagacacctt tgtcaaaaat gtgatcgatt
     1021 ttgtcgggtg cgatccccgg tccatcatcg ttaacttcgc agtgcagcca gccgtgacgg
     1081 tagtgcaatg ttacgctaat ttcgccaccg ggttccggcc ctaatgcttc cagcgcgttt
     1141 tctatcagat ttcccaatgt ggtaatcagc gtcgcgacct ggtcctcact gccgctgtct
     1201 ggcagctggc tttcactgtt taaaatcagc gtatggccta aatcggtcgc gcgattaatc
     1261 ttgctgatta aaaaaccagc gataaccgga gatttgatct tacccagcag agagccaatc
     1321 tcttcctgat agttattggc tgtttttaga atgtaatctt ccaactgctt ataactcttc
     1381 agatgcaata atccgagaat cacgtgcaat ttattcataa attcgtggga tcgttcacga
     1441 agtgcgtcgg catagttgac cagaccgtcg agtcgctgca tcagtttacg tacttcggtt
     1501 ttgtccctga aggttgaaat ggcaccgatg ataacgccat tactgcgcac cggaacggtg
     1561 ttgatcagta atagccggtc tttaatcgta atctcttcgt cgcggcgcgg cgtaccgtcg
     1621 cgtaacactt ccgagacatc taccacctgt gaccatgagt ggctgagcgt cgacagtttc
     1681 tcatcgtcct gcgacttacg gtaattcagc aattcttgtg cggcatcgtt gatcagcgtg
     1741 acctcgccgc gatcgtccac ggcaacgacg ccttctttga tagactgcaa catggcctgg
     1801 cgttgctcaa acagcgtgga gatttcgtag ggttccaggc cgaaaaggat ttttttcagt
     1861 accttaacca gaatgcaggt gccaatcagt ccgaccagca tgccaaataa taccgaccag
     1921 ataatgctcc agcgactgtc attgatttgt tgggtcacgc ggcttaactc aaggccgatc
     1981 gccaccacgc caatttgttt atgattttca tcgtagatgg gggtaaatac gcgtaaagcc
     2041 tgcgccagaa aaccgcgatt gatagcgaca ttttctttgc cattcagcgc tttaaggatg
     2101 tcatcacctt taaatggctg accaatacgc tgggcttcag gatgcgagta gcgaagactt
     2161 tgcatatcgg taacgacaat aaacagcaga tcgttgcgtt tgcgcacggc ttccgcgatg
     2221 gcctggatgc cgctctcctg cggttttttc tgcaagccct gacggatttc cggcgagttg
     2281 gcgagggtac gcgccactgc cagtgccttg ttggctagcc cgtctcgcgt catatcactg
     2341 atttgcgaga agtaaatcag atgcaccacc aatagcaccg agaacagtac cgcactgacc
     2401 attaagatca ctgtggtact caatttcatc ggacgtttgc gtaacatgcg gtagggcaat
     2461 gaatgtctca tcagcttcct tgtgtgacaa atttcttaaa cattatctct gatgaggtgg
     2521 gtaattcaaa gggagtaaga atgattggct atatagggga agagattctg gcaacggaaa
     2581 ctgccagtgc tgtatgaaga ttccagggct atgcttatag cgataatcat actgatgaga
     2641 gagggaaagt catggatcag gcgctactgg acgagggtta tcgctgttat accggcgaaa
     2701 agatcgatgt ctatttcaac actgcgatat gtcagcattc tggcaattgc gtacgtggca
     2761 acggcaagtt atttaatctc aaacgaaagc cgtggatcat gccggatgaa gtcgacgtcg
     2821 ccactgtggt taaagtgatt gatacgtgcc cgagcggcgc gctgaaatac cgtcataaat
     2881 aagcgagggt aaaatggaaa tacgcgaagg ccacaataaa ttttacatta atgacgaaca
     2941 aggcaagcaa atcgctgaaa ttgtctttgt gccgactgga gagaatttag cgattatcga
     3001 acataccgat gtcgatgaaa gcctgaaagg gcaggggatt ggtaaacagc tggttgcaaa
     3061 agtcgtggaa aaaatgcgtc gggaaaaacg aaaaattatc ccactatgcc catttgcgaa
     3121 acacgaattt gataaaacgc gggagtatga tgatattcgc agttgatggg agagtacaga
     3181 gtcacgatat ttttcatgct ctccgcggag tgatgcagga gagcatctga agggtagggg
     3241 gatgcacaaa gaatgggcag agagcgcgtt tttttgtccc aagtcatccc ctttactgag
     3301 caaaaaaaag aatatctcct atatgagaat catcaatcgg ggttatcaag ttttgcgtcc
     3361 ccagagcatt caatattgat aggagtcata ttatggaagg taaaaacaag ttcaatactt
     3421 atgttgtttc ttttgattat ccatcatctt attcctcagt gttcttaagg ttaagatcat
     3481 tgatgtatga tatgaatttc tcttctatcg tggctgatga atatgggata ccacgacaat
     3541 tgaatgaaaa ttccttcgca ataacgacat cgttagccgc aagtgaaatc gaagatttaa
     3601 tcaggcttaa atgcttagac ttaccggata ttgattttga cctcaacatt atgacagttg
     3661 atgactattt ccgtcagttt tacaagtagc caggaaggat aagaagaaca taaaaaatgg
     3721 cactattctc taaaatatta attttttatg tgattggtgt gaacatatcc tttgtcatta
     3781 tctggtttat ctcacatgag aaaacacata ttcgtttact tagtgcattc ctggtcggaa
     3841 taacctggcc aatgagtctg cctgtggcat tacttttttc tctcttttag gagctgtagt
     3901 tgagtggacc cgctggttat cgtgaaacga agcgtctctc ctcaaaactg gatttgcgga
     3961 acgacgaacg aagcagtcgc ctgactgctt cgttcattaa agtgaaattt atttctgtgg
     4021 gcgcatcgcc gggaagagaa taacgtcgcg aatagtatga ctgtttgtaa acagcataat
     4081 cattcggtcg ataccaatac ccagaccagc ggttggcggc agaccatatt ccagcgcagt
     4141 tacgtaatct tcgtcataga acatggcttc gtcgtcacct gcagctttag cgttaacctg
     4201 ttcctggaaa cgttcagcct gatcttctgc gtcgtttaat tcgctaaaac cattaccgat
     4261 ttcacgacca ccgatgaaga attcaaaacg gtcggtgatt tccgggttaa catcattacg
     4321 gcgcgccagc ggtgacactt ctgccggata ttccgtaata aaggttggct gaatcagatg
     4381 tgcttctgcc acttcatcaa agatctccgt gataatacgt cccagccccc agcttttctc
     4441 taccgtaatt ccgatagatt cagctaatgc tttagcagca tcaaaattat ccaggtcggc
     4501 catatcggtt tctggacgat attttttgat tgcttcgcgc atggtgagtt tttcaaacgg
     4561 tttgccgaaa tcaaacacat gctcgccata agtgacttta gtggtaccca gaacctcttg
     4621 tgccagggtg cggaacagtg actctgtcag ttcaatcaaa tcgtggtaat ccgcatacgc
     4681 catgtagagt tccatcattg tgaactcagg attatggcga acagaaatac cttcattacg
     4741 gaagttacgg ttgatttcga atacccgttc aaaaccgcct acaaccagac gtttcagata
     4801 cagctccggc gcgatacgca gatacatatc taaatccaga gcattatgat gggtaataaa
     4861 cgggcgagca gatgccccac ctggaattac ctgcatcatc ggggtttcta cttccataaa
     4921 gccgcgcgcg accatgaact gacggatagc ggccagaatt tttgaacgga caacaaacgt
     4981 ttgacgggat ttatcgttag cgatgaggtc cagataacgt tgacgataac ggacttcctg
     5041 atcctgcaga ccatggaatt tatctggtaa aggacgtagt gctttagtca gcaggcgcaa
     5101 ctcagtacag tgaatggaaa gctcacccgt ttgcgtctta aacagcgtac cgcgggcacc
     5161 gataatgtca cccagatccc attttttaaa ctgatcgtta taaacacctt ctggcaggct
     5221 atctcttgca acgtacagtt gaatacggcc accgacatcc tgcaacgtta caaaggaggc
     5281 tttccccatg atacgacggg tcatcattcg gccagcaacc gagacttcaa tgtttaagga
     5341 ttccagttcc tggttattct tcgcatcaaa ctcttcgtgc aactggtcag aggtatggtc
     5401 gcggcgaaaa tcattgggaa acgccacacc ttgctgacgt agtgccgcca gtttttcgcg
     5461 gcgatttctc agttcatcgt taaaatcaat agcctcattg gctccccgtg tttcttgttc
     5521 agacattttg gttcctctaa atccagcttt caatttagcg tagataaaga gacagat
//
LOCUS       DAZOZD010000256         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOZD010000256 DAZOZD010000000
VERSION     DAZOZD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.259"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUPB_003813"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUPB_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0760511.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUPB_003814"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUPB_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR0760512.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHUPB_003815"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHUPB_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0760513.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUPB_003816"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUPB_003816"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0760514.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUPB_003817"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUPB_003817"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0760515.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHUPB_003818"
     CDS             4447..4812
                     /locus_tag="ACHUPB_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0760516.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHUPB_003819"
     CDS             4962..5480
                     /locus_tag="ACHUPB_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0760517.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1520 a         1391 c         1240 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZOZD010000257         5433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOZD010000257 DAZOZD010000000
VERSION     DAZOZD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.260"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(197..292)
                     /locus_tag="ACHUPB_003820"
                     /pseudo
     CDS             complement(197..292)
                     /locus_tag="ACHUPB_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(289..855)
                     /locus_tag="ACHUPB_003821"
     CDS             complement(289..855)
                     /locus_tag="ACHUPB_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0760518.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            1165..2937
                     /gene="aspS"
                     /locus_tag="ACHUPB_003822"
     CDS             1165..2937
                     /gene="aspS"
                     /locus_tag="ACHUPB_003822"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0760519.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            3055..3507
                     /gene="nudB"
                     /locus_tag="ACHUPB_003823"
     CDS             3055..3507
                     /gene="nudB"
                     /locus_tag="ACHUPB_003823"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0760520.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            3536..4276
                     /locus_tag="ACHUPB_003824"
     CDS             3536..4276
                     /locus_tag="ACHUPB_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0760521.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            4311..4832
                     /gene="ruvC"
                     /locus_tag="ACHUPB_003825"
     CDS             4311..4832
                     /gene="ruvC"
                     /locus_tag="ACHUPB_003825"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0760522.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4834..>5433)
                     /locus_tag="ACHUPB_003826"
     CDS             complement(4834..>5433)
                     /locus_tag="ACHUPB_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0760523.1"
                     /translation="NINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGHN
                     GEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRK
                     LYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLG
                     SIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1363 a         1314 c         1478 g         1278 t
ORIGIN      
        1 agtgagggaa agtgattttt tggggcatgc atggggcaaa aatatttgtt gtggggcatt
       61 ttggggcatg attgggacac tttgttacat atgaactttg ccgattttca tatggcatgg
      121 caatccaacg ctttgattat tctaaagaaa acacatgctc ttgggcgttc tttagtgatt
      181 tttaaaaatc ccgtatcacg cagttaaagt ggcgggcata ctcttcaagg ctggtgatcc
      241 ccaaccgcac ccatttagga tgcgaccatt gcggtagacc gatgtaaatc ataacgcgtg
      301 aaggatctct tccacgctac gcacacgggc gatgcgcggg tagatatgat taatgctgtt
      361 attgtgttgc tcggcgctgg cggcactaca ggcgtcttcg gcaatcacca gattaaagcc
      421 aagttcccag gcattgcggg cggtggattc aacaccgata ttggtcgaga tcccacataa
      481 cactattgta tcgatacctc ggcggcgcag ttgcaactcc agatccgtac cgtaaaacgc
      541 accccactga cgtttgatga tttcgatatc actgtcagtt gcacctaatg cagctggatg
      601 ttgccaccag ttttcgggca acactttagc gggggagggg gcatcaaccg gctgttttaa
      661 tgcttcggcg taatcggcag accagccaac tcgcaccaga aacacgggct gaccgctggc
      721 gcgaaatttc gccgccagct tcccggcgcg attaactacc tcatcggcag tatgtggacc
      781 accggcaaaa ggtaagatgc cttcttgtaa atcgatcacc accaacgcag tggttttagc
      841 attaagttca agcatgctga ctcctgaaaa aacaaaatcg tgcctcacac cttaatgcga
      901 ttagccgatg gcggaacgac aaattttgtt aatttttatg agttcgggca acacctttat
      961 agcaaactgg cgtagagtct gatttcaggc ccgatataaa gtggtaacga ggcgttacag
     1021 cgcgggccag gctcttacgg tgcggcagaa tttccagtat aatagccgcc ttttttcatt
     1081 cagttgtgac atacagctaa cgctgcgact tggtcacctg cgcagcaggg ggcatccgcc
     1141 ggcagctaag ttaagggata tctcatgcgt acagaatatt gtggacagct ccgtttgtcc
     1201 cacgtggggc agcaggtgac tctgtgtggt tgggtcaacc gtcgtcgtga tcttggtagc
     1261 ctgatcttca tcgatatgcg cgaccgcgaa ggtatcgtgc aggtattttt cgatccggat
     1321 cgtgcggacg cgttaaagct ggcctctgaa ctgcgtaatg agttctgcat tcaggttacg
     1381 ggcaccgtac gtgcgcgtga cgaaaaaaat attaaccgcg atatggcgac cggcgaaatt
     1441 gaagtgctgg cgtcctcgct gactatcatc aaccgcgcag atgttctgcc gcttgactct
     1501 aaccacgtca acaccgaaga agcgcgtctg aaataccgct acctcgacct gcgtcgcccg
     1561 gaaatggcgc agcgcctgaa aacccgcgct aaaatcacca gcctggtgcg ccgttttatg
     1621 gatgaccacg gcttcctcga catcgaaact ccgatgctga ccaaagccac gccggaaggc
     1681 gcgcgtgact acttggtgcc ttctcgtgtg cacaaaggta aattctacgc actgccgcaa
     1741 tccccacagt tgttcaaaca gctgctgatg atgtccggtt ttgaccgtta ctatcagatc
     1801 gttaaatgct tccgtgacga agacctgcgc gctgatcgtc agcctgaatt tactcagatc
     1861 gatgtggaaa cttctttcat gaccgcgccg caagtgcgtg aagtgatgga agcgctggtg
     1921 cgtcatttgt ggctggaagt gaagggcgtg gatctgggcg atttcccggt aatgaccttt
     1981 gcggaagcag aacgccgtta tggttctgat aaaccggatc tgcgtaaccc gatggaactg
     2041 accgacgttg ccgatctgct gaaatctgtt gagtttgcag tatttgcggg gccggcgaac
     2101 gatccgaaag gccgtgttgc cgctctacgt gttccgggtg gcgcatcgct gacccgtaag
     2161 cagatcgacg aatacggtaa cttcgttaaa atctacggcg cgaaaggtct ggcttacatc
     2221 aaagttaacg aacgcgcgaa aggtctggaa ggtatcaaca gcccggtagc gaagttcctt
     2281 aatgcagaaa tcatcgaagc catcctggat cgtactgccg cgcaagatgg cgatatgatt
     2341 ttcttcggtg ccgacaacaa gaaaattgtt gccgacgcga tgggcgcact gcgcctgaaa
     2401 gtgggtaaag accttggtct gaccgacgaa agcaaatggg caccgctgtg ggttatcgac
     2461 ttcccgatgt ttgaagacga cggtgaaggc ggcctgacgg caatgcacca tccgttcacc
     2521 tcaccgaaag acatgactgc tgcagaactg aaagctgcac cggaaaatgc ggtggcgaac
     2581 gcttacgata tggtcatcaa tggttacgaa gtgggcggtg gttcagtacg tatccataat
     2641 ggtgatatgc agcagacggt gtttggtatt ctgggtatca acgaagagga acagcgcgag
     2701 aaattcggct tcctgctcga cgccctgaaa tacggtactc cgccgcacgc aggtctggca
     2761 ttcggtcttg accgtctgac catgctgctg actggcaccg acaatatccg tgacgttatc
     2821 gccttcccga aaaccacggc ggcagcgtgt ctgatgactg aagcaccgag ctttgctaac
     2881 ccgactgtac tggctgagct gagcattcag gttgtgaaga aggctgagaa taactgatat
     2941 gactcaaata cacgaaatca ttcgcgttgc atcaaggcgg caactgagtg aactcccatg
     3001 agcatagata actatgtgaa tgggatgagc gaaggcagtc aacgaagagg cagcgtgaag
     3061 gataaagtgt ataagcgtcc cgtttcgatc ttagtggtca tctatgcaca agatacgaaa
     3121 cgggtgctga tgttgcagcg gcgtgacgat cccgatttct ggcagtcggt aaccggcagc
     3181 gtggaagagg gtgaaaccgc gccgcaagct gccatgcgcg aagtaaagga agaggtcacc
     3241 attgatgttg tcgctgaaca actgacctta attgactgtc agcgcacggt agagtttgaa
     3301 attttttcac atttacgtca tcgctatgcg ccgggcgtga cgcgtaatac ggaatcatgg
     3361 ttctgtcttg cgctcccgca cgaacgccag gtagtcttca ctgaacatct ggcttacaag
     3421 tggcttgatg cgcctgctgc cgcggcgctc actaagtcct ggagcaaccg gcaggcgatt
     3481 gaacagtttg taattaacgc tgcctgaaca ggcagggcta tctggagata tttttatggc
     3541 aggtcatagt aaatgggcca acaccagaca tcgtaaagct gcgcaggatg ctaagcgcgg
     3601 taaaatcttc actaaaatca ttcgtgagct ggtaaccgcg gctaagctgg gcggtggcga
     3661 tccggacgct aacccgcgtc tgcgtgcggc ggtggataaa gcactgtcta acaacatgac
     3721 ccgtgacaca ctgaaccgcg caattgcacg tggtgtgggc ggtgatgatg atgcaaacat
     3781 ggaaaccatc atctacgaag gttacggtcc tggcggcacg gcaatcatga ttgaatgtct
     3841 gtctgacaac cgcaaccgta ccgttgctga agtgcgtcat gcatttagca aatgtggcgg
     3901 taacctcggt actgatggtt ccgttgccta tctgttcagc aaaaaaggcg tgatctcctt
     3961 cgagaaaggc gatgaagaca ccattatgga agcagcactg gaagcaggtg ctgaagacgt
     4021 tgtgacctat gatgacggcg cgattgacgt ctacactgca tgggaagaaa tgggtaaagt
     4081 gcgcgacgct ctggaagcgg caggtctgaa agcagacagc gcggaagttt ccatgatccc
     4141 gtccaccaaa gctgatatgg atgcagaaac cgcaccgaaa ctgatgcgtc tgatcgatat
     4201 gctggaagat tgcgacgacg tgcaggaagt ctaccataac ggtgaaatct ctgatgaggt
     4261 tgcagcaact ctctgatggg gcctgctaaa cagcaaaacg gagacgcgtg atggctatta
     4321 ttctcggcat tgacccgggt tcgcgcgtga tcggctatgg tgtcatccgc caggtaggca
     4381 ggcaactgtc ctacctgggt agcggatgca tacgcaccaa agtggatgat ttaccgtctc
     4441 gtctgaagct catctatgcg ggcgtgacgg aaatcatcac ccagttccag ccagattatt
     4501 tcgccattga acaagtcttt atggccaaaa atgccgactc ggctctgaaa ctggggcagg
     4561 cgcgcggcgt ggcgattgtg gcggcggtga atcaggaatt gccggtattt gaatacgcgg
     4621 cacgtcaggt aaagcaaacg gtggtaggta ttggtagtgc cgaaaaaagc caggtgcagc
     4681 atatggtccg caccttgctg aaactgcccg ctaatccaca ggcggatgcc gccgatgcgt
     4741 tagctatcgc gattacccat tgtcacgtca gccagaatgc aatgcagatg agcgaatcgc
     4801 ggctgaacct ggcgagaggg cgactgcgtt aagttatacc gcctcggtca gttcaggctg
     4861 aggcgtttca actccctccg cgtgaatcaa caccagaccc ggatgatgcc acaaactggc
     4921 tggcgtgacg gttttacgct cccacggaat agaacctaag aaccagaatt tccagaaagt
     4981 gagttttgca ttcggattgc tatttaacag ttctccaaac gtttctattt cacccaccgg
     5041 aatgcatagc gcttctttat aaatatcaaa aacaaatttt gaacagaact ggcgcgaaga
     5101 ttcgtattta aaaccggtgt ggtaaagttt gcgtagccgg gagggaacct gttcaacaat
     5161 tcgttgtttt tgttgttctg tcagcccagc gtctaatcgc tttatagcat agcgttgatt
     5221 agacgaacgt ttaataaaac gagatagcgt ggtgatggtt gaaaggggaa cccggctttc
     5281 tgcaaccagg aagtcttcac cgttatgacc gataatgatc ccgacgtgat tactccagca
     5341 atttgatgca gctgatattt gaccaaataa ggcagcacct atacatgtaa agacgatatc
     5401 tccaatttca tattcagcag ggtaattaat att
//
LOCUS       DAZOZD010000258         5384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOZD010000258 DAZOZD010000000
VERSION     DAZOZD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.261"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(183..326)
                     /gene="ypaB"
                     /locus_tag="ACHUPB_003827"
     CDS             complement(183..326)
                     /gene="ypaB"
                     /locus_tag="ACHUPB_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0760524.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            454..2739
                     /gene="nrdA"
                     /locus_tag="ACHUPB_003828"
     CDS             454..2739
                     /gene="nrdA"
                     /locus_tag="ACHUPB_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0760525.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2973..4103
                     /gene="nrdB"
                     /locus_tag="ACHUPB_003829"
     CDS             2973..4103
                     /gene="nrdB"
                     /locus_tag="ACHUPB_003829"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0760526.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4103..4357
                     /gene="yfaE"
                     /locus_tag="ACHUPB_003830"
     CDS             4103..4357
                     /gene="yfaE"
                     /locus_tag="ACHUPB_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0760527.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4411..5060)
                     /gene="inaA"
                     /locus_tag="ACHUPB_003831"
                     /pseudo
     CDS             complement(4411..5060)
                     /gene="inaA"
                     /locus_tag="ACHUPB_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(5140..5244)
                     /locus_tag="ACHUPB_003832"
                     /pseudo
     CDS             complement(5140..5244)
                     /locus_tag="ACHUPB_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5239..5384
                     /locus_tag="ACHUPB_003833"
                     /pseudo
     CDS             5239..5384
                     /locus_tag="ACHUPB_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1347 a         1437 c         1305 g         1295 t
ORIGIN      
        1 tttaacaata accgaataga aaacaaccat ttcgacatca acaatcgctt acattcgctt
       61 atatattgac cacaactgat acatcagatt atgtgatgac tcgtgcttag atcaattttt
      121 gcaatcatta acaaaaagat taataaacca tctatatcaa tttatccaac ctattctgcc
      181 gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat tgactttccc
      241 ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga gttatccaca
      301 aagttatgca cttgcaagag ggtcattttc acactatctt gcagtgaatc ccaaacatac
      361 cccctatata tagtgttcta agcagcttcc cttactacag gtagtctgca tgaaactatt
      421 gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct ggtgacaaag
      481 cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct ggattgggcg
      541 gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca cattcagttt
      601 tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc cgcagacctg
      661 atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat cttccacctg
      721 cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt ggtgaaaatg
      781 gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga agagttcaag
      841 cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc tgccgttaag
      901 cagctggaag gcaaatatct ggtacagaac cgcgtgaccg gcgaaatcta tgagagcgcc
      961 cagttccttt atattctggt tgccgcgtgc ctgttctcga actacccgcg tgaaacgcgc
     1021 ctgcaatatg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc gctgccgacg
     1081 ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt actgatcgag
     1141 tgcggtgaca gcctggattc catcaacgcc acctccagcg cgattgttaa atacgtttcc
     1201 cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag cccgattcgc
     1261 ggtggtgaag cgttccatac cggctgcatt ccgttctaca aacatttcca gacagcggtg
     1321 aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta cccgatgtgg
     1381 catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga aggcaaccgc
     1441 gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg tctgctgaaa
     1501 ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga cgcgttcttc
     1561 gccgatcagg aagagtttga acgtctgtat accaaatatg agaaagacga cagcatccgc
     1621 aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg tgcgtctacc
     1681 ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt tgatccggcc
     1741 atcgcgccag tgcgtcagtc taacctgtgc ctggagatag ccctgccgac caaaccgctg
     1801 aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc tttcaacctg
     1861 ggcgcaatta ataacctgga tgaactggaa gagctggcaa ttctggcggt tcgtgcactt
     1921 gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg agcgatgggt
     1981 cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa gcacggtaaa
     2041 cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc cattcagtat
     2101 tacctgctga aagcctctaa tgagctggcg aaagagcaag gcgcgtgccc gtggtttaac
     2161 gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga tctggatacc
     2221 atcgctaatg agccgctgca ttacgactgg gaagcattgc gtgaatcaat caaaacgcac
     2281 ggcctgcgta actctacgct ttctgccctg atgccgtccg agacctcttc gcagatctct
     2341 aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc gtcgaaagac
     2401 ggtattttgc gccaggtggt gccggactac gagcacctgc acgacgccta tgagctgctg
     2461 tgggaaatgc cgggtaacga tggctacctg caactggtag gtatcatgca gaaatttatc
     2521 gatcagtcga tctctgctaa caccaactac gatccgtcac gtttcccgtc aggaaaagtg
     2581 ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt caaaacgctg
     2641 tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt accgtcaatc
     2701 caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc cggatgcggc
     2761 gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc gccagcgtcg
     2821 catcaggctc cgggtgccgg atgcagcgtg aacgccttat ccggcctacg gctcggattt
     2881 gtaggcctga taagacgcgc cagcgtcgca tcaggcacag gatgcggcgt aaaatgcctt
     2941 atccggcatt aaactcccaa caggacacac tcatggcata taccaccttt tcacagacga
     3001 aaaatgatca gctcaaagaa ccgatgttct ttggtcagcc ggtcaacgtg gctcgctacg
     3061 atcagcaaaa atatgacatc ttcgaaaagc tgatcgaaaa gcagctctct ttcttctggc
     3121 gtccggaaga agttgacgtc tcccgcgacc gtatagatta ccaggcgctg ccggagcacg
     3181 aaaaacacat ctttatcagc aacctgaaat atcagacgct gctggattcc attcagggtc
     3241 gtagcccgaa cgtggcgcta ttgccgctta tttctattcc ggaactggaa acctgggtcg
     3301 aaacctgggc gttctcagaa acgattcatt cccgttccta tactcatatc attcgtaata
     3361 tcgttaacga tccgtctgtt gtgtttgacg atatcgtcac caacgagcag atccagaaac
     3421 gtgcggaagg gatctccagc tattacgatg aactgatcga aatgaccagc tactggcacc
     3481 ttctgggcga aggcacccac accgttaacg gtaaaaccgt aaccgttagc ctgcgcgagc
     3541 tgaagaaaaa actgtatctc tgcctgatga gcgttaacgc gctggaagcg atccgcttct
     3601 acgtcagctt tgcttgttcc tttgcctttg cagaacgcga actgatggaa ggtaacgcca
     3661 agattattcg cctgattgcc cgcgatgaag ccctgcacct gaccggtact cagcatatgc
     3721 tgaatctgct gcgcagcggc gcggatgatc cggagatggc ggaaattgcc gaagaatgta
     3781 agcaggagtg ctatgacctg tttgtccagg cggcacagca agagaaagac tgggcggatt
     3841 atctgttccg tgacggttcg atgattggtc tgaataaaga tattctctgc cagtacgttg
     3901 aatacatcac caatatccgc atgcaggcag tcggtttgga tctgccgttc cagacgcgct
     3961 ccaacccgat cccgtggatc aacacctggc tggtgtctga taacgtgcag gttgctccgc
     4021 aggaagtgga agtcagttct tatctggtcg ggcagattga cgcggaagtg gacaccgacg
     4081 atttgagtaa cttccagctc tgatggcccg cgttaccctg cgcatcactg gcacacaact
     4141 gctgtgccag gatgaacacc cttcccttct ggcggcgctg gaatcccaca atgttgcggt
     4201 tgagtaccag tgccgcgaag gttactgcgg ctcctgtcgc acccgactgg tcgcaggtca
     4261 ggtggactgg attgccgaac cgttagcctt tattcagccg ggggaaattt tgccctgttg
     4321 ttgccgggca aaaggcgata ttgaaatcga gatgtgaatt ggttgtagtg ccagatacaa
     4381 cgcttatacg tcttatctgg cctacaacga ttacatggcg tagtaatacg ctttcacctg
     4441 ctcccagtcc gctttgggga ttggctccag atattttcca gctggcggaa gtcatgatta
     4501 atcgctttat tgcgacgcaa gcggcgacga cttttctcca ggtcaagaaa accagcttct
     4561 gctttacctt ctgttttcac atagatatgg cgaacataac aacagccatg ctgacgatta
     4621 acgctatgca ttttcttaaa cgccagcgcc actgctttca acatggcttg ccgtacttcg
     4681 tcagaatagg gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     4741 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     4801 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     4861 acaatcgttg gtcggccgaa cggataacgt acggaatgaa acagatgatg cgtcatgcgc
     4921 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     4981 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     5041 tactttgctg aaactgccat accctatcgc ctgtcatttt tattaacgac aatgactata
     5101 ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat taatgaattt cttttgccgc
     5161 tactagtgtg acaccgcttt tgcccttcat ttgcagcgca tagattccac cagccacgac
     5221 cactaatagt gcgcccagca gataaaatgt gaatttaatc aggtggatac agcgatgaat
     5281 tttattcgac agggattagg catcgcctta ttaccagagt taacgctgaa aaccattgca
     5341 ggtgaattgt gttccgttcc tcacgaacca accttctatc gaca
//
LOCUS       DAZOZD010000259         5377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOZD010000259 DAZOZD010000000
VERSION     DAZOZD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.262"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUPB_003834"
     CDS             <1..108
                     /locus_tag="ACHUPB_003834"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0760528.1"
                     /translation="LRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            180..1538
                     /gene="mltD"
                     /locus_tag="ACHUPB_003835"
     CDS             180..1538
                     /gene="mltD"
                     /locus_tag="ACHUPB_003835"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0760529.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1587..2210)
                     /locus_tag="ACHUPB_003836"
     CDS             complement(1587..2210)
                     /locus_tag="ACHUPB_003836"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0760530.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2214..3014)
                     /locus_tag="ACHUPB_003837"
     CDS             complement(2214..3014)
                     /locus_tag="ACHUPB_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0760531.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3255..4169
                     /gene="yafC"
                     /locus_tag="ACHUPB_003838"
     CDS             3255..4169
                     /gene="yafC"
                     /locus_tag="ACHUPB_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0760532.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4166..4969)
                     /gene="dkgB"
                     /locus_tag="ACHUPB_003839"
     CDS             complement(4166..4969)
                     /gene="dkgB"
                     /locus_tag="ACHUPB_003839"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0760533.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5132..5208)
                     /locus_tag="ACHUPB_003840"
     tRNA            complement(5132..5208)
                     /locus_tag="ACHUPB_003840"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5172..5174),aa:Asp,seq:gtc)
BASE COUNT         1422 a         1290 c         1327 g         1338 t
ORIGIN      
        1 ttaagaacgg aagatattga tttaattaat gtaattaatg aagaaacatt attgcaacaa
       61 cctgaagagc gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt
      121 gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta agcaactatt gacacacaca
      181 tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggttgccag agtaccggca
      241 acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta
      301 caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg
      361 ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga
      421 aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt
      481 atatgtactg gatagcaggg caagttaaaa aacgtaacat gcctatggaa ctggtactac
      541 tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gccgcaggca
      601 tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg
      661 acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaatatgatg cagcgtctga
      721 acaaaatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc
      781 gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt
      841 taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc
      901 tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg
      961 cgcgtgtgca cctgagtagc ccggttgaaa tggcgaaggt tgcagatatg gcggggattt
     1021 ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg
     1081 ggccgcagta cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctcgctt
     1141 caggcgaaat tgctgctgta cagtcgacgc tggttgccga caatacgccg cttaacagcc
     1201 gtgtttacac cgtacgctct ggcgacacgc tttcaagcat cgcttcacgt ctcggcgtaa
     1261 gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa
     1321 gtttgacgat tggtgcaggc agtagcgcac agcggttggc aaacaacagc gatagcatta
     1381 cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca
     1441 tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc gataagctga
     1501 cgttgtttgt gaaaaacaac aacatgccag attcctgaca aaccagataa taaaaaaggc
     1561 accgattccc ccggtgcctt ttttatttat gccgctttat gtgcttctac catgatgata
     1621 tcactggtga aagagccatc agtctgcaag gaaaaatacg ttctcacctc tgtcgatgcg
     1681 ctctgttggt aaatgcgaat agcgtctact aacgcttctg gcgtacgcat tctcacgacc
     1741 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     1801 tcattgatta acgtcaacca cgcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
     1861 aatgcttcta ccgcctgtaa ccagatgtcg cgcactgggt gacccggaga cattacatcc
     1921 atcacaatca gcctaccgcc aggtttcaat atcctattca cttctcgcag tgctgcacca
     1981 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     2041 aatggcagac ttgcggcata tccctggcgg gtggtgatat ttttcagttg ccgggcttcg
     2101 gcagcttgtg ccacgacatc cagcatgtgg gcagataagt catacgccac caccgcgctc
     2161 acgttttgcg cagcgccaaa cgttggtctg ccctgcggca gacctgacat accttattta
     2221 tcaggcttgc cgggactgaa ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc
     2281 agtacagaag cttcactgac gttcagacca cggtagaaaa caaaatcgag cgggcgacca
     2341 aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc gcagcgacat ttcccgcgca
     2401 aagcgatata acgcgttcat ccttctacgg ctccaggcat tgaaatctcc cgccataatg
     2461 accgggccgc tgtggtgagc tatctgatca ccaataggaa gtaactgctt actatagaca
     2521 tccacgccca gactgaagtt gacggcgtgt atattaacca ccatcaacag gcgggtgtca
     2581 ggtaatggat agaccgtcac cagtgccgac ttcgccagac gcaaaatggg ttctcgttca
     2641 cgtaacgggc agcaatacac tggatgtgcc gccgaaaggg tcattacgcc agaaggatgt
     2701 tgtggcagca cgaaagcggg tacctgatcg gcggcaagat agttagcggt cgcaaactgt
     2761 accaactctg gcgttgtttg cgcttcctgc aataacacca gatgtgcgtc tttgccgtaa
     2821 ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt atatgttcca caccaggatc
     2881 cgaatacgct cttcggtact taacggttct ccgggtggta atgcctggcc gatgctcgca
     2941 aaagaccccg gcggtaagat cctctccgca ggttgtccgg caacatagcg catggcatag
     3001 gtgttttttc gcacttttga ctttgaaacc tctattacgt caacccttcc gcactcggga
     3061 gggttctact gttataggga ttttagctca ctctttcaac gagcaattgt atattgttat
     3121 gtaagcaagt gcttacaccg gtcagaaatt tgctcattat tgagaacatt aaatcggatg
     3181 acatatcacg catcctgatg gtttaatgac aaataaattc aaaatatctc atctattttg
     3241 caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg
     3301 aaagcggcag ctttagccgg gcagcggaac aattagggca agcaaactca gcggtaagcc
     3361 gggcggtgaa aaagctggaa atgaaacttg gcgttagcct gcttaatcgg accacgcggc
     3421 aacttagcct gacggaagaa ggcgagcgtt attttcgtcg cgtacagtca attttgcagg
     3481 agatggcagc ggcagaatca gaaattatgg agacgcgtaa tacaccgcgt ggactgttac
     3541 ggatcgatgc cgcaactcca gtggtgctgc actttctgat gccgttaatt aagcctttcc
     3601 gtgaacgcta tccggaagtc actttatcgc tagtctcctc cgaaacgatt attaatttga
     3661 tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt aacggactcc agcttacgtg
     3721 ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc ccccgattat atttcccgct
     3781 acgggaagcc agaaacgatc gacgatttaa agcaacatgt ttgcctggga ttcactgaac
     3841 ccgcttccct caatacctgg ccgatagcct gtagtgatgg acaattacat gaggtgaagt
     3901 acggtttgtc atccaatagt ggggaaacac tgaaacagct ttgcctgagt ggcaacggga
     3961 ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt
     4021 taatggcaga taaagtgttg ccagtggaaa tgccattcag tgccgtctat tacagcgacc
     4081 gtgcggtaag tacgcgcatc cgggctttta tcgatttcct tagcgagcat gtaaaaacag
     4141 ctcccggagg agctgtcaga gaggcttaat cccattcagg agccagacct tccgggctaa
     4201 ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt atcttcggca tcaagctgta
     4261 aattttgtgc cttaagatta ctttccaggt ttttacgttt agtagaagaa ggaattactg
     4321 agtaaccttc ccccatagcc cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag
     4381 ctgcaatacg agcaataacc tcatctttca gtgccttacc ataagccagc gtcatatagg
     4441 aagtgatatg aataccgtgc tgtttagccc aggcaaccac tttacggttt tgcagataag
     4501 gagagagttc aatctggttg gtagcgatgt tttcagcgcc aacagcagca atcgcttttt
     4561 ccatcaacgg gatcgtgaag ttggaaatac cgatctcacg tgtcagacct tgttttttgg
     4621 cctccagcag cgcctgcata aactcttcaa cagagacttc atcgtttggt gacggccagt
     4681 ggattagcgt cagatcaaca taatcggtac gcaatttttg caggctctct tttagactcg
     4741 ggatcaattt gtctttgctg agattttcaa tccagatttt agtggtgatg tagagttcat
     4801 gacgtggcac gccactttct gcaatcgcct gacctactgc ggcttcgtta tcatagattt
     4861 gtgcagtatc aattgcgcga taaccaagtt caagcgccgt tttcacagat gaaataacaa
     4921 cgtcgtcttt gagacggaaa gtacctaaac caaatgcagg gatagccata atatgcctct
     4981 tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc agactgttgc ggaaaaagag
     5041 cataaaatgc agaggatttt tgcgattctg gcaataatag atatacagac gaaaaaaaac
     5101 ctcgagcgtt aactcgaggc ttcttaataa gtggcggaac ggacgggact cgaacccgcg
     5161 accccctgcg tgacaggcag gtattctaac cgactgaact accgctccac cgaattcttt
     5221 tacaaccacc ggttttatga ccggcttact gcttaatttg atgcctggca gtttatgaat
     5281 cacttcgggc tgttgcttcg caacgttcaa aagctttcgc ttttgtccta ctctcttatc
     5341 gagtagtgaa ctgcctcaaa ggtcagtgcc attcttc
//
LOCUS       DAZOZD010000260         5373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOZD010000260 DAZOZD010000000
VERSION     DAZOZD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.263"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1034..1387)
                     /locus_tag="ACHUPB_003841"
     CDS             complement(1034..1387)
                     /locus_tag="ACHUPB_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFR0760534.1"
                     /translation="MLTSKEAKNNANNHSLQACQSGTWRKSNSGSTVITGRIANGQQI
                     PLPTGFSASQCSWSVSNAENPQGWKPNYFAGSVATYDANRIVKCGFYDEYNFHKGTFR
                     ADLTGKCSYVVACQN"
     gene            1386..2540
                     /locus_tag="ACHUPB_003842"
     CDS             1386..2540
                     /locus_tag="ACHUPB_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0760535.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKAITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            2691..3515
                     /gene="traE"
                     /locus_tag="ACHUPB_003843"
     CDS             2691..3515
                     /gene="traE"
                     /locus_tag="ACHUPB_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFR0760536.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLINDSSNTEVIFWHSFPFAGNTKREYCGVILRQEKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            3601..4803
                     /locus_tag="ACHUPB_003844"
     CDS             3601..4803
                     /locus_tag="ACHUPB_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFR0760537.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDVDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            4863..>5373
                     /locus_tag="ACHUPB_003845"
     CDS             4863..>5373
                     /locus_tag="ACHUPB_003845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
                     /protein_id="HFR0760538.1"
                     /translation="MMKPRSSYSKTAFILLFSVFLVAAVTKAKSSLPDITLEQAKEIN
                     ADNTVIFLFRHGERCDRSDMPCYSDKSGITITGTEKAQQEGIKFATIFSEYDIYSSNA
                     VRTIQTAKFFSGKEPVVMDSLSDCNNDLYKTLESIARESHKRNIVIMTHNHCLSFLAR
                     DRLGKKFKPA"
BASE COUNT         1546 a         1265 c         1152 g         1410 t
ORIGIN      
        1 aaaggtagga tctggtgagc ttcaaattgc tacagcacag gccacaggtt ggcgctttcc
       61 tggagcaaca gctacctgtc caactggaaa acgtgttaca ggtggcggcg gaatatgcac
      121 ctcacgaact ggttacatat ggcttaccag gtcattccca tcagccaata actcatggtc
      181 ggctgcgtgc gatacaaccg aagatcagaa tggttcaata actgtatatg ccatttgcca
      241 ataagctatt tcaagcaagt aatcttcata aagtaggacg aatactgtgt gtagtttttg
      301 gcttgccaag tcatctcgcc gttagagtca ggcatgtcaa taggaacgac ttctgtttct
      361 gccatttcac gcccgtcaat acgataggtg ttaatacaca gtttaaacct ccctaagacc
      421 tgtgtgtttt tgggatataa cgtaaatgtt tttatattcg tccatatcga cgctgatgat
      481 gatttccagg taccggattg gcactggcct acgtgccaat ccggtacctg gggcgctcct
      541 aaaattcaat tcacaacgca gacctacaac atagctaaaa acactcgtaa tttaaggctc
      601 ggggtccatg catattgctc atggacttac ctgaatggct caccctttgg tggttttcaa
      661 caggtatatt ccgaccaaaa caacgtttgg tatgtgagta attatgcttg gggaaattat
      721 gagtctggtg ggaccatatc agtcacatgc ctcaatcttc ctggtgctgg agcttaatta
      781 aaggggcaac agtaggcatc gacatgttcc tcatcaacac catcaccaat accgtacatg
      841 cgtgttccag tcataatgta tccagtcgga cattgaatcg gcttgtaata gatcgaacca
      901 ccactcttac cgccgataaa gtgattcatt gctacggatg atttgtacca attacatgct
      961 gagtaattaa ttttattacc ccctgaccac cgaccggatt ggcacgtagg ccagcagcgc
     1021 cagcaattta atctcagttc tgacacgcga cgacataact acattttccg gttaaatcag
     1081 ctctaaatgt ccctttatgg aaattgtatt catcataaaa accacattta acaattcgat
     1141 ttgcatcata agttgcaact gatcctgcaa agtaattcgg cttccaacct tgagggtttt
     1201 ctgcattgct cacactccat gagcactgac tggcagaaaa tcctgttggc agcggtattt
     1261 gctgaccatt tgctatacga ccagttatca ccgtactacc agaattgctt ttacgccaag
     1321 taccggattg gcacgcttgc agagaatgat tgttggcatt attttttgcc tcttttgagg
     1381 ttagcatgcc gtctccacgc atccgtaaaa tgtccctgtc acgcgcactg gataagtacc
     1441 tgaaaacagt ttctgttcac aagaaagggc atcaacagga gttttaccgg agcaatgtta
     1501 tcaagcgata tcccattgct cttcggaata tggacgaaat aacaaccgtt gatattgcta
     1561 catacagaga cgttcgttta gcagaaataa acccccgaac gggtaaagcc attacaggta
     1621 atactgtacg tcttgaactc gcccttctgt catctctgtt caatattgct cgtgttgaat
     1681 ggggaacctg tcgtactaac ccggttgaac tggttcgcaa gccgaaagta tcctccggac
     1741 gagatcgccg gctaacgtct tcagaagaac gtcgcctttc tcgctatttc cgcgaaaaaa
     1801 atctgatgtt gtatgtcatt ttccatcttg cccttgaaac agccatgcgg cagggcgaaa
     1861 tactggcctt acgttgggag cacattgatt tgcgccacgg tgtggctcat ttacctgaaa
     1921 ccaaaaacgg tcactcacgg gatgttcctc tgtccagacg tgcccgtaac tttcttcaaa
     1981 tgatgcccgt taatctccac ggcaatgttt ttgattacac cgcatccggc tttaaaaatg
     2041 cctggagaat agccacacaa cgacttcgca tcgaggacct gcattttcac gatctacggc
     2101 atgaagcaat aagccgcttc ttcgaactgg gtagcctgaa tgtaatggag attgctgcaa
     2161 tatcaggaca tcgttccatg aatatgctga aacggtatac tcatcttcgt gcatggcaac
     2221 tggtcagtaa gcttgatgcc cgccggcggc agacacaaaa agtggcagca tggtttgtgc
     2281 cgtatcctgc ccatatcacg actatcgatg aagaaaatgg gcagaaagcg catcgtattg
     2341 agatcggtga ttttgataac cttcacgtca ctgccacaac aaaagaggaa gcagttcacc
     2401 gcgccagtga ggttttgttg cgtacactgg ccattgcagc acagaaaggc gaacgtgtcc
     2461 catctcccgg agcgttacct gttaacgacc ctgactacat tatgatttgc cctctgaacc
     2521 cgggcagcac accgctgtaa acatcattca aaacacatac agaaaggcga gttacaacaa
     2581 ctcgccaaac acattcgatc tgcatgatcg aaattattcg tttcaccaga aaaaacaccg
     2641 aagatacaat aaccacatga gctgaaaaga ctttttcagt cggaggaaca atgagactga
     2701 atacgaccgg tattgcggcc cgaatgatgc tctcgctgga caaaaaagca atcggcgaaa
     2761 aactcatcaa cggtcgtcag ataaacccga caccactgca ggtccgttac ccacaagggg
     2821 taagagaagt tctggggata atgagcgaac aattagccat atccaccgct gatttaaccc
     2881 gaatcctgct tgaggatgct cttcataata tgtttatgcc tgcggataac accacaggaa
     2941 acataataag cagaattgaa tacatcatgc tgtcgcatga tataaatgcg ccattactgg
     3001 caacattgct gtcgccatgg aatatccgct ccacggttat acaggacccg gccagacttg
     3061 cagactatct ttcagctgac gctctcgaac atcttgctga atgttttaat ctcaatccgg
     3121 actggctgaa tggccatgaa aactatccga tagcgttgtc cggtgaatgg ccagatactg
     3181 ctgataattt cagaatgctt attaatgata gcagcaacac tgaagttatt ttctggcata
     3241 gtttcccctt cgcaggaaac acgaaaagag aatattgcgg ggtaatattg agacaggaaa
     3301 aagaaataaa tggctctgta atatatccgg cactatcatt atcaccaaca cttctgaacg
     3361 atgaaaaaag aaaatggtta actgaatata caaccaggca gaatacaaca atgtctttgc
     3421 gacgcgtcac gttgagacct ggccttgccg ggaaccttat tacagggcaa atactacctg
     3481 ttagcttatt taatacgtct ttattaccct ggtaaatacc agatagtacc aaagtaaaaa
     3541 attacggcac atccgtgtca tagcgttgtt ttgtctaaat tctgataaac aggaaattaa
     3601 atgatgaaga aaaatcatat tacccgcacc atcattgcat ctgctgtatt attttccttt
     3661 aacgctgcag cagcgacgtc atattttgaa gcgcgtaacg atgcaatggg tggcaccggg
     3721 gtagcatctt cccattatgg tgtggcacct ctggcaaacc cggctttact gacaaagcac
     3781 aacagcaatg atgatttcag ccttcttctg ccgtctgttg gggcacaagt tgctgatcct
     3841 gatgatgtat caaataaagc tgatgatgtt aaagatgact gggatttgtt tgacagtgct
     3901 gtagataacc agcatggcgt acaacaggca gcggcaaatc ttaaacatcg tctgcaggaa
     3961 ttccgcaata tcaatgcaga tgctcaggta ggtgtatcag ctgtagcagc catggcaaat
     4021 gacactctgc cgtttgccct tatggtcaag tcttacggaa ctgtcagcgt aaacggaaaa
     4081 gtaaacgatg cagacctgga ttatctggac aaagttgcca atggaacaat taccgacgtc
     4141 gataagaatg ccctgacctc acgcgcattc gggcgtgcag ctgtaattac cgacgtcgga
     4201 atttcatttg ccaaagaact ggaaacggcc ggacaaaaat ggtctcttgg cgtcacacca
     4261 aaatatcagc gtgtggacct gtttaactac aacgtaacgg ttcgtgatta cgacaaagac
     4321 gattttgacg gtgacaagta ccacaacacg aaaaacggat tcaacgccga tattggtgct
     4381 tatacagacc tgaatgacaa ctggactgtc ggtcttgttg cccagaacat cattccacgc
     4441 agtattgata ctaaagtcgt caatggcttt aaagaaacct tcaaagttcg tccacaggca
     4501 accgccggtg tatcctggca taatgacctg tttaccactg ctcttgacgt tgatctgact
     4561 cctgccagtg gcttcacttc tgacagcaaa cgtcagtttg caagtgttgg tgctgaattt
     4621 aacgcatgga aatgggcgca attgcgtgcc ggttatcgtc agaacatggc atcaaactcc
     4681 ggaagcgcct tcaccgctgg cgttggtatc tctccatttg acgtcgtaca tatcgatgtt
     4741 tccggcctgg taggaactga ccacgattac ggtgcaatgg cacaacttca gttcactttc
     4801 tgattacagt ccccgccccc ggcactaaac ccgggggtat ttttatattc agtttaaata
     4861 acatgatgaa accacgttct tcttattcaa agacagcatt cattctttta ttttctgttt
     4921 tcctggtagc tgcagtgaca aaagcaaaaa gctcgcttcc cgatatcacc ctggaacagg
     4981 caaaagaaat taatgctgat aacaccgtta ttttcctttt ccgccatgga gaacgttgcg
     5041 accgttcaga tatgccctgc tattcggata agagcggaat cactattacc ggtacagaga
     5101 aagcacagca ggaaggtatt aagttcgcaa caatcttcag tgaatatgat atttactcca
     5161 gcaatgcggt aagaaccata caaactgcaa aattcttctc cggtaaagaa cccgtcgtta
     5221 tggacagttt atcagactgc aataatgatc tttataagac gcttgaatca atcgcaagag
     5281 aatctcataa aaggaacata gtaataatga cacataatca ttgtttatca tttctggcca
     5341 gagataggct cggcaagaaa ttcaaaccgg cgt
//
LOCUS       DAZOZD010000261         5351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOZD010000261 DAZOZD010000000
VERSION     DAZOZD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.264"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            219..665
                     /locus_tag="ACHUPB_003846"
     CDS             219..665
                     /locus_tag="ACHUPB_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0760539.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            676..2205
                     /locus_tag="ACHUPB_003847"
     CDS             676..2205
                     /locus_tag="ACHUPB_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR0760540.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            2195..3265
                     /locus_tag="ACHUPB_003848"
     CDS             2195..3265
                     /locus_tag="ACHUPB_003848"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0760541.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3265..5013
                     /locus_tag="ACHUPB_003849"
     CDS             3265..5013
                     /locus_tag="ACHUPB_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR0760542.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(5062..>5351)
                     /locus_tag="ACHUPB_003850"
     CDS             complement(5062..>5351)
                     /locus_tag="ACHUPB_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR0760543.1"
                     /translation="FDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETK
                     KYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         1241 a         1362 c         1389 g         1359 t
ORIGIN      
        1 gttatatttc cctgatacat tgtgagtaaa tcacaaaaat aatgaataac ccattaatga
       61 ttcatgtggt ttatttaaat aaccattatg tgcattactc cgcaaatctg acctttcact
      121 ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc atcacttaat cgctccacca
      181 ttacaattga ttaaaatata ttttatggag tccgattaat ggcagctctt actgcaagct
      241 gtattgacct gaatattcag ggcaatggcg cttattccgt tctgaagcag ttggcgacaa
      301 tagcgttaca aaacggtttt atcaccgact cacaccagtt cctgcaaacc ctgctgctgc
      361 gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc cgtgccgcac ggtaaaagcg
      421 cctgcgttaa acaaccgttt gtattattcg cccgcaaagc gcaggctatt gactggaaag
      481 ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg cgtgccgcaa agcggcgaag
      541 aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat tatccaccag gattttattc
      601 atcaactgca acagggcgac gccgaccagg tgcttgcctt gttaaatcaa accctcagct
      661 cataaggaag tggcgatgga gtcatcctta tgtattgtcg cgatcaccaa ctgccccgcc
      721 gggatcgctc acacctacat ggtggcggaa gccctggaac agaaagcccg ttctctcggc
      781 cataccataa aagtggaaac tcaagggtcc agtggcgttg aaaaccgctt atccagcgaa
      841 gagattgccg ctgccgatta cgtcattctc gctaccgggc gtggcctgag cggtgatgat
      901 cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca tctcccaggc gttgaaaaat
      961 atcgaccaga ttttcagcga attaccgaca aactcgcagc tttttgccgc agatagcggc
     1021 gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga tgagccacct gatggcgggc
     1081 gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc tggtggcgct tgccaacatg
     1141 ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag gcgcgccgtc gtttacctgg
     1201 gtagttgaat ccatcggtta ccttggcttc acctttatga tccccatcat gggcgcttat
     1261 attgcctcgt cgattgccga taaaccggct ttcgctccgg cgtttctggt ttgctatctg
     1321 gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg gattcctcgg cgcggtggtg
     1381 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
     1441 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
     1501 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
     1561 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
     1621 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
     1681 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
     1741 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
     1801 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
     1861 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
     1921 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
     1981 ggtggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
     2041 ggtatctttg acccgctgat cggcctgatg tcgccggtgg gttcgtttta tctggtgcta
     2101 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
     2161 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
     2221 gtgcgaagcc agcgccgtca gcggcgatga acaggaagtt cgcgacattc tgataaacac
     2281 gctggaacct tgcgttaatg agatcacctt tgatggtctg ggcagctttg ttgcccgtaa
     2341 ggggaataaa ggtccaaaag ttgccgttgt caggcatatg gatgaagtcg gctttatggt
     2401 cacccacatc gacgagagcg gttttctgcg ctttaccacc attggcggct ggtggaatca
     2461 gtcaatgctc aaccaccggg taaccatacg cacacacaag ggatttaaaa tccctggtgt
     2521 gattggttcc gtcgcacctc atgcgttaac ggaaaagcaa aagcaacagc cgctgtcatt
     2581 tgatgagatg ttcattgata ttggcgcgaa cagtcgcgat gaggtggaaa aacgcggtgt
     2641 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
     2701 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
     2761 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
     2821 acgcggggca caaacctcgg cggaacacat taaaccggat gtggtgattg tgctggatac
     2881 cgctgtcgca ggtgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
     2941 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtggcggc
     3001 gttaaaaagc tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
     3061 tgcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
     3121 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
     3181 tctgctcacg ttgatacggg attttctgac caccttaacc gcggagaaag tcaacgcgtt
     3241 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
     3301 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
     3361 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
     3421 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
     3481 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tacctttact
     3541 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cctgggtagc agagcgtctg
     3601 ccccggttaa aacagcgtta tgaacgcact tgttgcctgg ccagtcgccc cggtttgggc
     3661 catttcattg atgagacaga agagaaacgc gttatcttgc tggcgaactt gctgcgcaaa
     3721 gatccgtttt taattccgct ggcgggcgta acacgagaca attttcagca tttatccacg
     3781 gcctgcgaca accaacaccg ctggccgctc atgcagggtg attatctctc cagcctgatt
     3841 ctggcgattt acgccctgcg taatcaactg accgatgagt ggccgcaata tcccggtaac
     3901 gagataaaac aaatcgttga acagagcggc atgtttcttg gtgataacgc tgtaagaacc
     3961 ctgacaggtt tgatagagaa acagcatcag caagcgcagg taatttcagc cgatcatgtg
     4021 ctggggttgc tgcaaagggt gccaggcatc gcgtcattga atattattga tacgcagctg
     4081 gttgagaata ttaccgggca tttattacgt tgccttgccg caccggtgtg gattgccgag
     4141 caccgccaaa gcagcatgaa taacctgaaa gccgcctggc ctgcggcctt tgatatgagt
     4201 ctgcacttta ttacgctact gcgtgaacag ctcgatattc cccttttcga tagcgatctg
     4261 atcggtttgt attttgcctg tgcgctggaa cggcatcaaa acgaacgcca gccgatcatt
     4321 ttgctctcgg accagaacgc gattgccact attaatcagc tcgccattga gcgcgatgtt
     4381 ttacattgtc gggtgattat tgcccgtagc ttaagcgagc ttattgccat tcgcgaagag
     4441 attgagccgt tattgatcat taacaacagc cattatttac tggatgacgc ggtaaataat
     4501 tacatcaccg taaaaaatat cattacggct gccgatatcg aacaaataaa acactttctg
     4561 gcgacggcat ttattcgcca acagccggaa cgctttttct ctgcccccgg aagttttcat
     4621 tattcgaatg tacgcggtga aagctggcaa catattaccc ggcaaatttg tgcgcaatta
     4681 gtggcacaac accatattac cgccgatgaa gcgcaacgga tcatcgcccg cgaaggcgaa
     4741 ggtgaaaacc tgattgttaa tcgcctcgcc atcccacatt gctggagcga acaggagcga
     4801 cgttttcgtg gattttttat taccctcgcc caaccagttg aggtgaataa cgaagtcatt
     4861 aaccatgtct tgatcgcctg cgccgccgcc gatgcgcgtc atgagctaaa aatatttagc
     4921 tatctggcaa gcgtattgtg tcagcatccg gcagaagtta ttgccgggtt aacaggatat
     4981 gaggcattta tggagttact tcacaagggg tgaactgtgt cggatgcgcc gcatccgaca
     5041 ctgcattttc atcaaaaacg tttaacgcag gctattatag tcatcaacca tttcgttgta
     5101 ctcgcgtaac gctcgtggaa atgagtcttc gaccatccag cttgtgtttg catcctgaag
     5161 atgctcttta tcccagtcag agtacgggac tttatctctg acgcggcgaa catatttttt
     5221 agtctcgagt tgatactggt ctgccgaatt aatgaacgag aaattcatac cgcctttatc
     5281 cgcttctttg gcctgcgcta ccagcgtttc cagttcagag actttcttca ttgccgcatc
     5341 aacatcaaac t
//
LOCUS       DAZOZD010000262         5342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOZD010000262 DAZOZD010000000
VERSION     DAZOZD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.265"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUPB_003851"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUPB_003851"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0760544.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUPB_003852"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUPB_003852"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0760545.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUPB_003853"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUPB_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR0760546.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUPB_003854"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUPB_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0760547.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1266 g         1330 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tt
//
LOCUS       DAZOZD010000263         5338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOZD010000263 DAZOZD010000000
VERSION     DAZOZD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.266"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            361..447
                     /gene="leuL"
                     /locus_tag="ACHUPB_003855"
     CDS             361..447
                     /gene="leuL"
                     /locus_tag="ACHUPB_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0760548.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            540..2111
                     /gene="leuA"
                     /locus_tag="ACHUPB_003856"
     CDS             540..2111
                     /gene="leuA"
                     /locus_tag="ACHUPB_003856"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0760549.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDITTAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2111..3202
                     /gene="leuB"
                     /locus_tag="ACHUPB_003857"
     CDS             2111..3202
                     /gene="leuB"
                     /locus_tag="ACHUPB_003857"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0760550.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3205..4605
                     /gene="leuC"
                     /locus_tag="ACHUPB_003858"
     CDS             3205..4605
                     /gene="leuC"
                     /locus_tag="ACHUPB_003858"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0760551.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4616..5221
                     /gene="leuD"
                     /locus_tag="ACHUPB_003859"
     CDS             4616..5221
                     /gene="leuD"
                     /locus_tag="ACHUPB_003859"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0760552.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5270..>5338)
                     /locus_tag="ACHUPB_003860"
     CDS             complement(5270..>5338)
                     /locus_tag="ACHUPB_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760553.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1371 a         1408 c         1396 g         1163 t
ORIGIN      
        1 ttaatgcatt aattcttaac attaattgat caataatatt caccaaatca atatcaaaaa
       61 aaatcgcaaa acatataatt caatacaaat catcaggata ggttttgcaa cgcgtgcatt
      121 ttgtcccctt tttcctcgtt gattagatgc aaaaatttat gctgaaatat gtcaaccgat
      181 gaaaagcgtc ggtagttaag cagaaattaa tatcgcttac tttaaccacc gcagcacaat
      241 tagctaattt tacggatgca gaactcacgc tggcgggacg tttttattgc gtcagggttg
      301 acatccgttt ttgtatccag taactctaaa agcatatcgc attcatctgg agctgattta
      361 atgactcaca tcgttcgctt tatcggtcta ctactactaa acgcatcttc tttgcgcggt
      421 agacgagtga gcggcatcca gcattaagcc agcacgcagt caaacaaaaa acccgcgcca
      481 ttgcgcgggt ttttttatgc ccgaagcgag gcgctctaaa agagacaagg acctaaacca
      541 tgagccagca agtcattatt ttcgatacca cattgcgcga cggtgaacag gcgttacagg
      601 caagcttgag tgtgaaagaa aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg
      661 acgtgatgga agtcggtttc cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca
      721 tcgcccgcca ggttaaaaac agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata
      781 tcgacgtggc ggccgaatcc ctgaaagtcg ccgaagcctt ccgtattcat acctttattg
      841 ctacctcccc aatgcacatc gccaccaagc tgcgcagcac cctggacgag gtgatcgaac
      901 gcgctatcta tatggtgaaa cgcgcccgta attacaccga tgatgttgaa ttttcttgcg
      961 aagatgccgg gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg
     1021 ccggtgccac caccatcaac attccggaca ccgtgggcta caccatgccg tttgagttcg
     1081 ccggaatcat cagcggcctg tatgaacgcg tacctaacat cgacaaagcc attatttccg
     1141 tacataccca cgacgattta ggcctggcgg tcggcaactc actggcggcg gtacatgccg
     1201 gtgcacgcca ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc
     1261 tggaagaagt catcatggcg attaaagttc gtaaggatat tctcaacgtc cacaccgcca
     1321 ttaatcacca ggagatatgg cgcaccagcc agttagttag ccagatttgt aatatgccga
     1381 tcccggcaaa caaagccatt gttggcagca gcgcattcgc tcactcctcc ggtatccacc
     1441 aggatggcgt gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa tctattggtc
     1501 tgaaccaaat ccagctgaat ctgacctctc gttcggggcg tgcggcggtg aaacatcgca
     1561 tggatgagat ggggtataaa gaaagtgaat ataatttaga caatttgtac gatgctttcc
     1621 tgaagctggc ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg ctggccttca
     1681 tcggtaagca gcaagaagag ccggagcatt tccgtctgga ttacttcagc gtgcagtctg
     1741 gctctaacga tatcaccacc gccgccgtca aactggcctg tggcgaagaa gtcaaagcag
     1801 aagccgccaa cggtaacggt ccggtcgatg ccgtctatca ggcaattaac cgcatcactg
     1861 actataacgt cgaactggtg aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc
     1921 tgggtcaggt ggatattgtc gccaactaca acggtcgccg cttccacggc gtcggcctgg
     1981 ccaccgatat tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac aatatctggc
     2041 gtgccgcaga agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa aacaacaagg
     2101 aaaccgtgtg atgtcgaaga attaccatat tgccgtattg ccgggggacg gtattggtcc
     2161 ggaagtgatg acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct ttgcgatgcg
     2221 catcaccacc agccattacg atgtaggcgg cgcagccatt gataaccacg ggcaaccact
     2281 gccgcctgcg acggttgaag gttgtgagca agccgatgcc gtgctgtttg gctcggtagg
     2341 cggcccgaag tgggaacatt taccaccaga ccagcaacca gaacgcggcg cgctgctgcc
     2401 tctgcgtaag cgcttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct
     2461 ggaagcattc tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca tcctgtgcgt
     2521 gcgcgaactg accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca
     2581 atatgaaaaa gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg
     2641 catcgcgttt gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa
     2701 cgtgctgcaa tcctctattt tatggcgtga aatcgtcaac gagatcgcca cggaataccc
     2761 ggatgtcgaa ctggcgcata tgtacatcga caacgccacc atgcagctga ttaaagatcc
     2821 atcacagttt gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg
     2881 cgcaatgatc actggctcga tggggatgtt gccttccgcc agcctgaacg agcaaggttt
     2941 tggtctgtat gaaccggcag gcggctcagc accagatatc gcaggcaaaa acatcgccaa
     3001 cccgattgcg caaattctgt cgctggcact gctgctgcgc tacagcctgg atgccgatga
     3061 tgcggcttgc gccattgaac gcgccattaa ccgcgcatta gaagaaggca ttcgcaccgg
     3121 agatttagcc cgtggcgctg ccgccgttag taccgatgaa atgggcgata tcattgcccg
     3181 ctatgtggca gaaggggtgt aatcatggct aaaacgttat acgaaaaatt attcgacgct
     3241 cacgtagtgt acgaagccga aaacgaaacc ccgctgttat atatcgaccg ccacctggtg
     3301 catgaagtga cctcaccgca ggcgttcaat ggtctgcgcg cccacggtcg cccggtacgt
     3361 cagccgggca aaaccttcgc cactatggat cacaacgtct ctacccagac gaaagacatt
     3421 aatgcctgtg gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa
     3481 tttggcgtcg aactgtatga cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg
     3541 ccggaacagg gcgtcacctt gccggggatg accattgtct gcggcgactc gcataccgcc
     3601 acccacggcg cgtttggcgc actggccttt ggtatcggca cttccgaagt tgaacacgta
     3661 ctggcaacgc aaaccctgaa acagggccgc gcaaaaacca tgaaaattga agtccagggc
     3721 aaagccgcgc cgggcattac cgcaaaagat atcgtgctgg caattatcgg taaaaccggt
     3781 agcgcaggcg gcaccgggca tgtggtggag ttttgcggcg aagcaatccg tgatttaagc
     3841 atggaaggtc gtatgaccct gtgcaatatg gcaatcgaaa tgggcgcaaa aaccggtctg
     3901 gttgcaccgg acgaaaccac ctttaactat gtcaaaggcc gtttgcatgc gccgaaaggc
     3961 aaagatttcg acgacgccgt tgcctactgg aaaaccctgc aaaccgacga aggcgcaact
     4021 ttcgataccg ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc
     4081 aatccaggcc aggtgatttc cgtgaacgac aatattcccg atccggcttc gtttgccgat
     4141 ccggttaaac gcgcgtcggc agaaaaagcg ctggcctata tggggctgaa accgggcatt
     4201 ccgctgaccg aagtggctat cgacaaagtg tttatcggtt cctgtaccaa ctcgcgcatt
     4261 gaagatttac gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag
     4321 gcactggtgg ttcccggctc tggcccggta aaagcccagg cggaagcgga aggtctggat
     4381 aaaatcttta ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg
     4441 atgaacaacg accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt
     4501 gaaggccgcc aggggcgcgg cgggcgcacg catctggtca gcccggcaat ggctgccgct
     4561 gctgctgtga ccggacattt cgccgacatt cgcaacatta aataaggagc acaccatggc
     4621 agagaaattt atcaaacaca caggcctggt ggttccgctg gatgccgcca atgtcgatac
     4681 cgatgcaatc atcccgaaac agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca
     4741 tctgtttaac gactggcgtt ttctcgatga aaaaggccaa cagccaaacc cggacttcgt
     4801 gctgaacttc ccgcagtatc agggcgcttc cattttgctg gcacgagaaa acttcggctg
     4861 tggctcctcg cgtgagcacg cgccctgggc attgaccgac tacggcttta aagtggtgat
     4921 tgcgccgagt tttgctgaca tcttctacgg caatagcttt aacaaccagc tgctgccggt
     4981 gaaattaagc gatgcagaag tggacgaact gtttgcgctg gtgaaagcca atccggggat
     5041 ccatttcgac gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt
     5101 taccatcgat gccttccgcc gccactgcat gatgaacggt ctggacagta ttgggcttac
     5161 cttgcagcac gacgacgcca ttgccgctta tgaagcaaaa caacctgcgt ttatgaatta
     5221 atccccttgc ccggtcaaat gaccgggctt tccgctatcg tccacgtcat caaacgtctt
     5281 taacctttgc ggttaaaaat aatgcgacaa cagaaataac cgcaattacc cagtagac
//
LOCUS       DAZOZD010000264         5296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOZD010000264 DAZOZD010000000
VERSION     DAZOZD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.267"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHUPB_003861"
     CDS             complement(<1..130)
                     /locus_tag="ACHUPB_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0760554.1"
                     /translation="MDTRVAFMTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPS"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="ACHUPB_003862"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="ACHUPB_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUPB_003863"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHUPB_003863"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR0760555.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUPB_003864"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHUPB_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0760556.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUPB_003865"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="ACHUPB_003865"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0760557.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="ACHUPB_003866"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="ACHUPB_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAZOZD010000265         5291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOZD010000265 DAZOZD010000000
VERSION     DAZOZD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.268"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(59..2842)
                     /locus_tag="ACHUPB_003867"
     CDS             complement(59..2842)
                     /locus_tag="ACHUPB_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0760558.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2899..5271)
                     /locus_tag="ACHUPB_003868"
     CDS             complement(2899..5271)
                     /locus_tag="ACHUPB_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0760559.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1317 a         1232 c         1209 g         1533 t
ORIGIN      
        1 cataaaaatg gcgaatattg ctaaaatccc cgccaacgat gtgttgacgg ggctgttatt
       61 attttggcaa taatactcca gtataagtat ttaccggatg agaaagatat tgtttaacgg
      121 cagtgttaac attctctacc gtcatttgtt tcaacaattg ctcctgctca gtccatgctg
      181 caggatcgtc atattgaata agactattta caatagtgtt cgctaattgt tgaacgctac
      241 gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcattcagt tcttgctcac
      301 tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac agttcatcat
      361 gtcgttctgg ttgacaagta aaagccagca aatgactgat atctttggcc tgaggatcaa
      421 ccgagaggcg agaagaaacg ctgtatgctc cagatgcctg ttcacgaata ttaacgcgta
      481 gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtcgcc agattaacag
      541 gtgtccggga atcataacgc ttccactgtg aaacctgtgc cacaggttca ttttgttctt
      601 ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg gctaatggcg
      661 aatcagagtg tttgattgat cctaagtaac gcgtaattaa cgccacgagt ttgtcttctg
      721 agacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac tggcgatcgg
      781 cagccagcgc atctgcggca gtaaactgta caatctgatt ttcttgcagt aatttcgtgc
      841 ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct ggacgctggt
      901 cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata ttgtcgttaa
      961 tcgtgctgtg ggtgattcgc tggttaatca actgaaaacc aggttcaggg ttattagtcc
     1021 gcgcgctaac ggagagcaac gtattcatgc cactgacttt actgctcatg gttaccgaat
     1081 tttccgcact ccagcgtttc aggctggaag aggagagctc accaacgccg cttccgctta
     1141 ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt aaatcgcctt
     1201 tattagatac ggcagtaatt tgtagctttt gctcttcacc cgcggatttt gccagaataa
     1261 ccctggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt tctttgctgc
     1321 taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct gggaagatgt
     1381 aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc gcaagagaca
     1441 atgctttttt ggcggcaagc tcattgttta ccatttgctc ccaaaatgcg tcctggttct
     1501 ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc cacagacgtt
     1561 tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat gagctggatg
     1621 ccaggcgact ggtcagcata cgtaaatcac gttcagcttg ctgatcaacc gcatttttca
     1681 gccaggtgag gcgggtagat ttgacatcat cgagttcttc agcagaaaaa ccatgctgat
     1741 caatggttgc caactctgcc attaatgcat ttgcagcatc ctgcatatta tcgtctcgtg
     1801 catttacacg gaaaaacagc gactgataat cgggtgcaat tttaacgctg cgcgcagtgc
     1861 cgccagaaat agtcttcaac tcgcccgact gtatgcgttc ctgcagacgt tgattgaaca
     1921 gctgaactaa catgctccat tcagcttgtt cgacaaagct ttgctcatcg ttcacttgta
     1981 ccattggcag gcgataatag agtgcgatgc cgttcactcg gttttcttta tcattaatga
     2041 tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca gctgctttgt
     2101 tagccggaag cttacttaaa ttatccttta tcagcgccag cgcttcttta ctgtcgatat
     2161 cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa aattggcgca
     2221 attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc gataggttca cggtctaaat
     2281 tacgggtatt tgccagcagg aaagggcggc gcgcctgaga ggtgcgccat ttcgcatcct
     2341 gatgggcacg ccattcctca gtaattacgc cgcgttcagc gtctacttcg agtatttcaa
     2401 aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga ttttgtttct
     2461 gcgtagtcgg cagactcacc tgatacagcg tttcgtcata gctggtatag gcattaacat
     2521 cgcgaccgaa acgcaggccc attgactcaa atgtttcgat gactttatta cccggccatg
     2581 tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc tcattgtctt
     2641 cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc tttggttgag
     2701 catgcggata aatcatatat cgcaagccat tgtccagttg cccggtaatt aacttttcat
     2761 cctgcggtaa ggcggcggca atcagccgcc cggggagcaa cagagttgcc actaacgtca
     2821 gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcgaa tttacccggt
     2881 taagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag tacggccact
     2941 ggcgtaggtc gccacaccgg tgttcgttgt atcaacagcg gttttgctat ccagtacatt
     3001 gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg tccaggacaa
     3061 gcgggtgtcc caggtcaggc tgctatccag tttttcgtca acatactgct tgtaatctga
     3121 atattcgctg atgtattgcg catttgtctt accaaggata atgcgagctt tacgcgcttc
     3181 ttgccaggcc agcgtattcg cccacactaa cccgctcggt tgatgcgtga aatccatgtt
     3241 taaggagatc tttaatgggt tattaaaatc tgccactgga acactatcgt aagagaccag
     3301 attaccgtta taaaccacct ggttatcacc ggtattgctc tcctcataac cattgtttaa
     3361 cgacaagttg cccttgctct tgatatagct aaagacaatt tgtgggttaa tatctacttg
     3421 gcggatatgc aggggttcgg ccagttcaaa actgaggctg aacgaatgcg ttttggtttt
     3481 gccatcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac gactgctttt
     3541 gctgatttga tcatgcgctt cacggtaaac atagtttgca cgcgcaataa cgttcttacc
     3601 gattttctgc tgcaatccca ttgccagttc atcgttataa ggcgttttca aatcctgata
     3661 acgcgtcaga gttttattac ctgataccga ttccgtccag ctattgcgga tatcacgtaa
     3721 tcccatatca agaatattcc cgccatagta acggttataa ccggcggtaa tcattgaggt
     3781 ttgatcagca aaaatatccc attccgtcat aaagcgcggg gagatattgt ggtttgacag
     3841 atagttgtca tagtcataac gcacaccggg cattaacgac acattccgcc agctaatgtg
     3901 ctccgccata tacagcgtgt agttgtcaat tcccaggctg cctttacctt tatggtaaat
     3961 ggtatggtta gttttctttc cggcagcatt aatcacatag gattcagact ggttatggcg
     4021 ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat gcgaaacatc
     4081 acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat ctacagcctg
     4141 ggaaatgtgt cctaatccgc cacaggtaca acgccctgta atatcaccat atgtacatga
     4201 aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat gatcccaacc
     4261 aacggtggta cgtagtttgg cccatgcgag ttgcgtatcc atatcccatg ccagaccata
     4321 ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat aatcacggct
     4381 ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa accaggtaaa
     4441 tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggtga caatgccgtc
     4501 attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac cggcggtaac
     4561 gccgaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct ttttaaaatc
     4621 tggggagtaa taagtacttc ctgaagagcc ttgattaaat gcgctcttgt tattctcatc
     4681 gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccaatttca ccttgctatc
     4741 atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc gaccaaattc
     4801 aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat caagataata
     4861 cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat tcgctgggtt
     4921 caggttatta gttgcactaa taccgtcaat caaataggca ttctggtagg gcgacgcacc
     4981 gtgaatagag attttctcag ggcgaatatc tccctggttc aacgaggtac tttgcgttga
     5041 atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat tcccggtcgg
     5101 taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta cagggacagg
     5161 agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttgtcat cgacggcctg
     5221 cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca tttatttata
     5281 tctccgtaca g
//
LOCUS       DAZOZD010000266         5265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOZD010000266 DAZOZD010000000
VERSION     DAZOZD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.269"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            110..967
                     /gene="focA"
                     /locus_tag="ACHUPB_003869"
     CDS             110..967
                     /gene="focA"
                     /locus_tag="ACHUPB_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0760560.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1022..3304
                     /gene="pflB"
                     /locus_tag="ACHUPB_003870"
     CDS             1022..3304
                     /gene="pflB"
                     /locus_tag="ACHUPB_003870"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0760561.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3496..4236
                     /gene="pflA"
                     /locus_tag="ACHUPB_003871"
     CDS             3496..4236
                     /gene="pflA"
                     /locus_tag="ACHUPB_003871"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0760562.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4368..4505)
                     /locus_tag="ACHUPB_003872"
                     /pseudo
     CDS             complement(4368..4505)
                     /locus_tag="ACHUPB_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            4605..>5265
                     /locus_tag="ACHUPB_003873"
     CDS             4605..>5265
                     /locus_tag="ACHUPB_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0760563.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1348 a         1302 c         1272 g         1343 t
ORIGIN      
        1 ccatgcgagt tacgggccta taagccaggc gagatatgat ctatatcaat ttctcatcta
       61 taatgctttg ttagtatctc gtcgccgact taataaagag agagttagtg tgaaagctga
      121 caaccctttt gatcttttac ttcctgctgc aatggccaaa gtggccgaag aggcgggtgt
      181 ctataaagca acgaaacatc cgcttaagac tttctatctg gcgattaccg ccggtgtttt
      241 catctcaatc gcattcgtct tctatatcac agcaaccact ggcacaggca caatgccctt
      301 cggcatggca aaactggttg gcggcatttg cttctctctg gggctgattc tttgtgttgt
      361 ctgcggagcc gatctcttta cttccaccgt gttgattgtt gttgctaagg cgagtgggcg
      421 catcacctgg ggtcagttgg cgaaaaactg gctaaatgtc tattttggca acctggtcgg
      481 cgcactgctg tttgtacttt taatgtggct ttccggcgag tatatgaccg caaatggtca
      541 atggggacta aacgtcctac aaaccgccga ccacaaagtg caccatactt ttattgaggc
      601 cgtctgtctt ggtatcctgg caaacctgat ggtatgtctg gcagtatgga tgagttattc
      661 tggccgcagc ctgatggaca aagcgttcat tatggtgctg ccggtcgcga tgtttgttgc
      721 cagcggtttt gagcacagta tcgcaaacat gtttatgatc ccgatgggta ttgtaatccg
      781 cgacttcgca tccccggaat tttggaccgc agtcggttct gcaccggaaa atttttctca
      841 cctgaccgtg atgaatttca tcactgataa cctgattccg gttacgatcg gcaacattat
      901 cggtggtggt ttgttggttg ggttgacata ctgggtcatt tacctgcgtg aaaacgacca
      961 ccattaatgg ttgtcgaagt acgcagtaaa taaaaaatcc acttaagaag gtaggtgtta
     1021 catgtccgag cttaatgaaa agttagccac agcctgggaa ggttttacca aaggtgactg
     1081 gcagaatgaa gtaaacgtcc gtgacttcat tcagaaaaac tacactccgt acgagggtga
     1141 cgagtccttc ctggctggcg ctactgaagc gaccaccacc ctgtgggaca aagtaatgga
     1201 aggcgttaaa ctggaaaacc gcactcacgc gccagttgac tttgacaccg ctgttgcttc
     1261 caccatcacc tctcacgacg ctggctacat caacaagcag cttgagaaaa tcgttggtct
     1321 gcagactgaa gctccgctga aacgtgctct tatcccgttc ggtggtatca aaatgatcga
     1381 aggttcctgc aaagcgtaca accgcgaact ggatccgatg atcaaaaaaa tcttcactga
     1441 ataccgtaaa actcacaacc agggcgtgtt cgacgtttac actccggaca tcctgcgttg
     1501 ccgtaaatct ggtgttctga ccggtctgcc agatgcatat ggccgtggcc gtatcatcgg
     1561 tgactaccgt cgcgttgcgc tgtacggtat cgactacctg atgaaagaca aactggcaca
     1621 gttcacttct ctgcaggctg atctggaaaa cggcgtaaac ttggaacaga ctatccgtct
     1681 gcgcgaagaa atcgctgaac agcaccgcgc tctgggtcag atgaaagaaa tggctgcgaa
     1741 atacggctac gacatctctg gtccggctac caacgctcag gaagctatcc agtggactta
     1801 cttcggctac ctggctgctg ttaagtctca gaacggtgct gcaatgtcct tcggtcgtac
     1861 ctccaccttc ctggatgtgt acatcgaacg tgacctgaaa gctggcaaga tcaccgaaca
     1921 agaagcgcag gaaatggttg accacctggt catgaaactg cgtatggttc gcttcctgcg
     1981 tactccggaa tacgatgaac tgttctctgg cgacccgatc tgggcaaccg aatctatcgg
     2041 tggtatgggc ctcgacggtc gtaccctggt taccaaaaac agcttccgtt tcctgaacac
     2101 cctgtacacc atgggtccgt ctccggaacc gaacatgacc attctgtggt ctgaaaaact
     2161 gccgctgaac ttcaagaaat tcgccgctaa agtgtccatc gacacctctt ctctgcagta
     2221 tgagaacgat gacctgatgc gtccggactt caacaacgat gactacgcta ttgcttgctg
     2281 cgtaagcccg atgatcgttg gtaaacaaat gcagttcttc ggtgcgcgtg caaacctggc
     2341 gaaaaccatg ctgtacgcaa tcaacggcgg cgttgacgaa aaactgaaaa tgcaggttgg
     2401 tccgaagtct gaaccgatca aaggcgatgt cctgaactat gatgaagtga tggagcgcat
     2461 ggatcacttc atggactggc tggctaaaca gtacatcact gcactaaaca tcatccacta
     2521 catgcacgac aagtacagct acgaagcctc tctgatggcg ctgcacgacc gtgacgttat
     2581 ccgcaccatg gcgtgtggta tcgctggtct gtccgttgct gctgactccc tgtctgcaat
     2641 caaatatgcg aaagttaaac cgattcgtga cgaagacggt ctggctatcg acttcgaaat
     2701 cgaaggcgaa tacccgcagt ttggtaacaa cgatccgcgt gtagatgacc tggctgttga
     2761 cctggtagaa cgtttcatga agaaaattca gaaactgcac acctaccgtg acgctatccc
     2821 gactcagtct gttctgacca tcacttctaa cgttgtgtat ggtaagaaaa cgggtaacac
     2881 cccagacggt cgtcgtgctg gcgcgccgtt cggaccgggt gctaacccga tgcacggtcg
     2941 tgaccagaaa ggtgcagtag cctctctgac ttccgttgct aaactgccgt ttgcttacgc
     3001 taaagatggt atctcctaca ccttctctat cgttccgaac gcactgggta aagacgacga
     3061 agttcgtaag accaacctgg ctggtctgat ggatggttac ttccaccacg aagcatccat
     3121 cgaaggtggt cagcacctga acgttaacgt gatgaaccgt gaaatgctgc tcgacgcgat
     3181 ggaaaacccg gaaaaatatc cgcagctgac catccgtgta tctggctacg cagtacgttt
     3241 caactcgctg actaaagaac agcagcagga cgttattact cgtaccttca ctcaatctat
     3301 gtaattagat ttgactgaaa tcgtacagta aaaagcgtac aataaaggct ccacgaaagt
     3361 ggggcctttt ttagcacgag agcctttttt gtcagctatc tatactctaa ggtgactgcc
     3421 aaaacagact cgacgtagcc ttcgagctgc gcaccaacac ggcctcagat gggccacatc
     3481 tggagaaaca ccgcaatgtc agttattggt cgcattcact cctttgaatc ctgtggaacc
     3541 gtagacggcc cgggtattcg ctttatcacc tttttccagg gctgcctgat gcgctgcctg
     3601 tattgtcata accgcgacac ctgggatacg catggcggta aagaagttac cgttgaagat
     3661 ttaatgaaag aggtggtgac ctatcgccac tttatgaacg cttccggcgg cggcgttacc
     3721 gcatccggcg gtgaggcaat cctacaagct gagtttgttc gtgactggtt ccgcgcctgc
     3781 aaaaaagaag gcattcatac ctgtctggac accaacggtt ttgttcgtcg ttacgatccg
     3841 gtgattgatg aactgctgga agtaaccgac ctggtaatgc tcgatctcaa acagatgaac
     3901 gacgagatcc accaaaatct ggttggagtt tccaatcacc gcacgctgga gttcgccaaa
     3961 tatctggcga acaaaaatgt gaaggtgtgg atccgctacg ttgttgtccc aggctggtct
     4021 gacgatgacg attcagcgca tcgcctcggt gaatttaccc gtgatatggg caacgttgag
     4081 aaaatcgagc ttctccccta ccacgagctg ggcaaacaca aatgggtggc aatgggtgaa
     4141 gagtacaaac tcgacggtgt taaaccaccg aagaaagaga ccatggaacg cgtgaaaggc
     4201 attcttgagc agtacggtca taaggtaatg ttctaatttc cccgtaaagc ggcaacttta
     4261 ttgagttgcc gcttttttat tccgcacttc attctgctac agcgttttta acacatctta
     4321 tagtgcatct accatatccc gtacctgaga taataacgac tgataatatt acgccgatat
     4381 aaatcgagtt ccgtcacctg tattttccgc tccattgccc gctgatggat cgcatcagca
     4441 atatgtgcgg tcaatgaatc atgacgagga tgggcccata ccaaataaat acgttcagac
     4501 tgcataattt tacctccacg ttaagttctg gagcgcactt tacgcaggcg gcataataat
     4561 gtaaacgtga tgattcagga tgagttaatg aaggattttc actaatgcgg atgaatatgt
     4621 ctgactttgc cactttcttt gctgtggccc gtaatcaaag ctttcgtgca gcgggcgatg
     4681 agttaggctt atcctcgtcc gccattagcc atagtattaa aacactggaa caacgtctta
     4741 aaattcgctt attcaatcgg acaacccgaa gcgtatcgtt aacggaagcg ggttctaatc
     4801 tctatgaacg attgcgccca gctttcgacg aaattcagat catgttagat gagatgaatg
     4861 attttcgtct gacaccaaca ggaacgctca aaatcaatgc cgcccgggta gctgcacgta
     4921 tatttttaat gcctttactg gttggtttca cacgtgaata tcctgatatt aaagtcgagc
     4981 tcacaactga cgactctctt gtcgatatcg tccaacaggg ctttgacgct ggcgttcgac
     5041 ttagtggcat cgtcgaaaaa gatatgatca gcgtggcgat cggcccacca gtgaaattat
     5101 gtgtagcagc aacgccagag tactttgcaa gatatggcaa accccgccat cctcatgatc
     5161 tgcttaatca tcaatgtgtt gtatttcgtt atcccagcgg caaacctttc cactggcaat
     5221 ttgcaaagga gcaggaaatt gctgtcgcag ggaatatcat tctgg
//
LOCUS       DAZOZD010000267         5243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOZD010000267 DAZOZD010000000
VERSION     DAZOZD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.270"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            204..551
                     /locus_tag="ACHUPB_003874"
                     /pseudo
     CDS             204..551
                     /locus_tag="ACHUPB_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            604..999
                     /locus_tag="ACHUPB_003875"
     CDS             604..999
                     /locus_tag="ACHUPB_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0760564.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1040..1783
                     /gene="cmoA"
                     /locus_tag="ACHUPB_003876"
     CDS             1040..1783
                     /gene="cmoA"
                     /locus_tag="ACHUPB_003876"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0760565.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1780..2751
                     /gene="cmoB"
                     /locus_tag="ACHUPB_003877"
     CDS             1780..2751
                     /gene="cmoB"
                     /locus_tag="ACHUPB_003877"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0760566.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2916..>5243)
                     /gene="torZ"
                     /locus_tag="ACHUPB_003878"
     CDS             complement(2916..>5243)
                     /gene="torZ"
                     /locus_tag="ACHUPB_003878"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0760567.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1180 a         1362 c         1279 g         1422 t
ORIGIN      
        1 gactataccg atactgcata gaatttgtgt aggattgcat agatacttgg aaaaacaccg
       61 ttaaattgca tagtcattca cctgatattg tcctgttttt acaggtagtt gattcggatg
      121 ttctaggaat catcctcata gttgattcgg ttagttgggc ttgtaccaac ccctaatcaa
      181 cctcatagtg tatttagacc ttgatgattt tagacagccg ccctgttcat gcagcacgtc
      241 cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg
      301 tactgacggc gacaaatccg ctgattcgtt ttatcggtag tgatgatatg acgcaaaacc
      361 gggaattatt tcaggtctgg ttacagaaat tagcgcagtg gcatcagacc actacgcctt
      421 atcttttttt acatacgcca gacattgccc aggccccgga actggtacat accctgtggg
      481 aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt
      541 ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt
      601 tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt tattaatgaa gttctctttt
      661 gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa
      721 ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat cgcgattgtg
      781 ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc
      841 gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct gtttcgctgg
      901 cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt
      961 tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt
     1021 tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag
     1081 actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata tgatccagcg
     1141 ttccgttccc ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt
     1201 tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt
     1261 gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact ccccggcgat
     1321 gattgaacgc tgcagtcgtc atattgacgc ctataaagcc cctacgccag tagacgtcat
     1381 tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac
     1441 cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt atcaaggact
     1501 gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg
     1561 tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga
     1621 gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca
     1681 taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa
     1741 ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt
     1801 tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt
     1861 gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cgcggtggaa
     1921 tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac cgccgaaagc
     1981 gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg
     2041 ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc
     2101 gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg caccattctg
     2161 gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc
     2221 gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta
     2281 ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct tccggcactg
     2341 aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc gccgctggag
     2401 catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct ggaaacgctg
     2461 gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt
     2521 aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt
     2581 gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg acgcactgaa
     2641 tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag taagacagtt
     2701 gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt
     2761 attactgccg gatgcggcgt gaatgcctta tccggcctac aaacccttgc taattcaatc
     2821 tattgcaggg actatgtagg cctgataagc atagcgcatc aggcagtttt acgtttgcat
     2881 aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc aggcttatca
     2941 aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag cgcagagtta
     3001 ccggcacagg catttgccag ctgcgagctg gggatatccg ccgttaacac gttcgcactg
     3061 ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac gcataccacg
     3121 ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg tttgttccag
     3181 acacgcacca gatcgccatt cgcaatacta aatcgagcag catcttcggt gtgaatagtg
     3241 atcggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg
     3301 tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc
     3361 ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt
     3421 tccagcgttt tggagtaaat ctcaattttg ccacttggcg tacccagcgc atttttcacc
     3481 ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct gcggcgcatt
     3541 tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac gcgttgcgca
     3601 cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct
     3661 tcggtataga tctcttttcc gccaggtttg agtaattccg caagatcggc aaacacatca
     3721 aaatcgtttc gcgcttcaaa ttgcggagca acagcctgct tcatcggcac aatatgctgg
     3781 ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat
     3841 acgatatctg cgtgtttagc agctgcggtc cagtagcatt cagaaacgac gatcatctcc
     3901 ggtttctgcc atgctttaat cagacgatta gtatcctggt gatgggtaaa gttaccgcca
     3961 cccgcccacc agatcatttt gatattcgga tacgtttgct ctttaccgtt atgctgatat
     4021 ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc cctcattccg
     4081 ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc cggcagcacg
     4141 ccgccaacac gtgtcgggtt gccaccattg gagtaatgat aagagaaacc aaaaccgccg
     4201 cccggtgtac caatctgccc taacatcgcc gccagcgtca ccagcatcca gtgtttctgt
     4261 tcaccatatt gctggcgctg aattccccag cctgccatca gcattgtgcg attcgccgcc
     4321 atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgcccag
     4381 gccgcgctct tcggcgtatt atcgctctta cctctcagat actcttcaaa ctgcggatac
     4441 ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc
     4501 gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca ggtggcatta
     4561 tcgccaaaga agtcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca
     4621 gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct ccaggcaatt
     4681 tttaaggtat tcagcgggtt catcccccac agcaccacaa cctggctgtt ttccagaatc
     4741 agcggccagg aggtctgctg ttcatacacc tctacagaac cgaccacatg cggcatgatc
     4801 acctgtgccg ccccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc
     4861 atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg ccagccgtat
     4921 gatccggcaa aaatagccga tggtccgtta gctttgcgaa tgcgatcgtg ttgctcatga
     4981 atcagtttta atgcttgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc
     5041 gtcggttgca gtggattatc gagatagctt tttctcacca tcggatgctg aatacgcgtc
     5101 gtggtgtgta cctgatccgc cgccgtagac tgtaaggaat tcggtatggt tttcgccagc
     5161 gcgcctgttg aagaaacaat cttgccgtcc ttcacttcta cgttcatcgc tccccaacgt
     5221 tccgcggtga ggattttacc gtc
//
LOCUS       DAZOZD010000268         5236 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOZD010000268 DAZOZD010000000
VERSION     DAZOZD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.271"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            120..1178
                     /locus_tag="ACHUPB_003879"
     CDS             120..1178
                     /locus_tag="ACHUPB_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760568.1"
                     /translation="MRMNRLPGYGLPELAFWPQPKYERNKWSMFCLKLRNDGTLAWYR
                     RYVDRGMPNHAVDDDYDNYPEARKAALELNKNVSFDIEKLPLSMFQKKSLRLKVDKAL
                     KAKSRLMDEEYIMLNEAIKAHANDPRVTLNELIINPDYETLRQPLFDVLSEMPYLHFV
                     FLPKFQVSLQLVAPNTWECSYNNKAKTAKICYQERIARGFGLSGAAHWGKTKATIRSM
                     LLPRANRLLQLASVKRMLDEALRNGQKVLVVGNFVFWFEDKNQVGWSVKVASESETTS
                     RGNTLWKEGTIISKNHGRIVVLPYTKENGKHVKGYTKNAPNDGNAIPRHKDEYVELPF
                     EILDGDLMFSLFGELNYE"
     gene            complement(1239..1592)
                     /locus_tag="ACHUPB_003880"
     CDS             complement(1239..1592)
                     /locus_tag="ACHUPB_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760569.1"
                     /translation="MKMGDLAKRKESDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(1598..2266)
                     /locus_tag="ACHUPB_003881"
     CDS             complement(1598..2266)
                     /locus_tag="ACHUPB_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0760570.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            complement(2429..3205)
                     /locus_tag="ACHUPB_003882"
     CDS             complement(2429..3205)
                     /locus_tag="ACHUPB_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760571.1"
                     /translation="MSPSFIAEYSKVLSDFPECEAREKIRVGYMVNSDFSVLRLTEWS
                     LIGSNYDYCLTVSFVGDPDADWLQEHAMDLDEQGHSEEEIVHSVRELVGDEQEDDDGI
                     QIARFVYKRFQFRSQKGSHLGVQIKGAFVTPSKEQKGLARRVYNFLLNWHDHVVCDDH
                     QTVYGARIWAVGMLLVGRVQIYDNLNKEFIDVLVEGGIGQNGVKPWDALLLNSEAQLR
                     HWNPMAVSIDPSSQILTIISKEDRHIEVGVTTFDARSTNH"
     gene            complement(3206..3604)
                     /locus_tag="ACHUPB_003883"
     CDS             complement(3206..3604)
                     /locus_tag="ACHUPB_003883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760572.1"
                     /translation="MHTALNASRSIQTIRDCTKMIASVPLRHWNDSLQIREMNKPGML
                     KDQRFIVNLKSDVEEGKFTIKLFAEMMVLNNRLRFLVKPEKQFPDVVRCEQDAFYEIE
                     SKIAKFIKDKYRVDVKIAKLDSSTDMRLAN"
     gene            complement(3930..4181)
                     /locus_tag="ACHUPB_003884"
     CDS             complement(3930..4181)
                     /locus_tag="ACHUPB_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760573.1"
                     /translation="MLGSLMIKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQSTVDVKNETLDELRRKDASTVHHELHNKWLRD"
     gene            complement(4183..4875)
                     /locus_tag="ACHUPB_003885"
     CDS             complement(4183..4875)
                     /locus_tag="ACHUPB_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760574.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGSSIDTIREVIERWAPPNENNTDTYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMA"
     gene            complement(4889..5212)
                     /locus_tag="ACHUPB_003886"
     CDS             complement(4889..5212)
                     /locus_tag="ACHUPB_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760575.1"
                     /translation="MANDVGGVTHDQVERIAKIVAREVVGKLGKEIREEIVHEVHEQL
                     KNYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIISKVTSFIITALILGLAAYGV
                     KNGLQ"
BASE COUNT         1419 a         1229 c         1075 g         1513 t
ORIGIN      
        1 tcctaatgga taccaagaac aacataattt gactccaact ccagatgcta tgtcattgac
       61 agatttatca agggagatca tgttagaatt agtgttttag cctcgtggat attgtcacca
      121 tgcgcatgaa tagacttcca ggatatggcc ttcccgaact ggctttttgg cctcagccta
      181 aatatgaacg gaataaatgg tcgatgttct gtctgaaact ccgtaatgat ggaacccttg
      241 cttggtatcg gcgatatgtt gatcgtggta tgccaaacca cgctgttgat gacgactacg
      301 ataactatcc tgaagcaaga aaagcggcat tagagctgaa taaaaacgtg agctttgaca
      361 ttgaaaaact tcctctatct atgtttcaaa agaagtcatt gcgtttgaaa gttgacaaag
      421 cactcaaagc caaatcacgt ctcatggatg aagagtatat aatgctcaac gaagcaatta
      481 aggcacacgc caacgatccg cgagtgacat taaatgagtt aataattaac ccagattacg
      541 aaactttgcg tcaacccctt tttgacgtgc taagcgagat gccttactta cattttgttt
      601 ttcttcccaa attccaagtt tcacttcaac tcgttgctcc taacacttgg gaatgttcat
      661 ataacaacaa agctaagaca gcgaagatct gctaccaaga acgcatagcc agaggttttg
      721 gtttatcagg tgctgcacat tggggaaaaa caaaagcgac tattcgctca atgcttctgc
      781 cgcgtgctaa ccggttactg caactcgcca gcgtaaaaag aatgctggat gaagctctca
      841 ggaatgggca aaaggtactg gttgttggca actttgtttt ctggtttgag gacaaaaatc
      901 aggtaggttg gagtgttaaa gttgctagcg aaagcgaaac cacctcaaga ggcaatacgc
      961 tctggaaaga aggaactatc atttcaaaaa atcacggtcg aattgtcgtg ctgccgtata
     1021 caaaagaaaa cggtaaacat gtaaaaggtt ataccaagaa tgcccctaac gacggcaatg
     1081 caattccgag acataaggat gagtatgttg agttgccctt tgaaattttg gatggtgact
     1141 tgatgttcag tctgttcggt gagcttaact acgaatagct ttcatagaac gaaatatttc
     1201 taaggctgtc caatgcagac cagccttaga gatttatatc aacctttctt cattaactcg
     1261 cgtttaattt catcggtacg catcgtgaca tcggcagcag tgatcgcctc gttaagcttc
     1321 acgatgtcct cgatttcctg cggtgacttc tctgccagat ggaaaatagc agcacgaatc
     1381 acatcagagc gggtgaattt ctcgaagcga gggatgaact tcatcatctc cagcagttcg
     1441 aagtattcgt cctccagtga cattgtgcgg cttttaattt tctctttgcc acgagtcggg
     1501 cgtccctgtg gtctgactgg ttggcgcaaa ggagttgtgt tcttagccgg tgcatcagac
     1561 tctttgcgct ttgctaggtc acccattttc atggacatta ttcttcttcc tccagactca
     1621 acagataatc tacaaattct tcaaactcgg cttccgcctt tttgtcgcgc tcgctaccgg
     1681 tcatttcaaa gatagaacga ccagactctt ccgcatcatc atagacgttg cggttatata
     1741 gattgactgg cgcagactcg atgccaaacg tctcaacaat ctctttagcc gccagaatgc
     1801 gagacacttg tgatggcaaa gccgggcact ggttcatgac cgcgcggacc ttcactttat
     1861 cgtttacatt gcgaacattg tcgataatag gatcgatgtc acgtaaagat ttcaaatcac
     1921 gacgcttagg acgcagcggg ataatgataa cgtcagccat cagcatcgct tgtcgctgaa
     1981 tttcggagtc gaagccacca gcatctacca ctacaaactc agctctaccc tgaagcgatt
     2041 ttaggtgctt aatgatgtca tcctgaacgt atgcaaaagg aatcagctca aggtcttcgt
     2101 tctgtcgacg gtcttcacac cagctcgttg tcgtgcgctg aatatctata tcggtaatat
     2161 aaaccttctt cttctttttg actttcaggc aaacggcaat ttgctgggca acggtggatt
     2221 tgccaggccc gccctttgtg ccgccaacca caaagatctt ggtcattgga gagtttcctt
     2281 tgcgtctatt attattgtct gaaacaactt gttttcttat atgtgatata gcctaaatgc
     2341 ctacggctgc tgtgtaaagg tttaatgata ggtacataga tttggtaagg acttaataac
     2401 aaaaaacccg ccgtagcggg tttaggaatt agtggttggt agacctggca tcaaatgttg
     2461 taacgcccac ttcaatgtgg cggtcttctt tggagatgat ggtgagaatc tgactagatg
     2521 ggtctatgct gacagccata gggttccagt gacgtaactg agcctcgctg ttcaatagca
     2581 gcgcatccca cggtttgacg ccattctgcc caataccacc ttcaacaagg acatctataa
     2641 attctttatt taaattgtca tagatttgga cacgaccgac caaaagcata cctacagccc
     2701 aaattctggc accgtaaacc gtctgatggt catcacatac tacatggtca tgccaattga
     2761 gaagaaaatt gtaaactctt ctagcaagtc ccttttgttc ctttgaaggg gttacaaatg
     2821 cccctttaat ctggacccca aggtgagagc ctttttgtga gcgaaactgg aatcgtttgt
     2881 agacgaacct ggcgatctga atgccgtcat catcttcttg ctcatcacca actaattcac
     2941 gaacactatg aactatctct tcttctgaat gcccctgctc gtcaagatcc attgcatgtt
     3001 cttgaagcca atctgcatca gggtcgccta caaatgacac tgtcaaacag taatcataat
     3061 tagacccgat caaggaccac tctgtgagcc gtaaaacaga aaaatcagag ttaaccatgt
     3121 agccaactct gattttctct cttgcttcac attcagggaa atcagacagt acttttgaat
     3181 actctgctat gaatgatggt gacatttagt tagcaagccg catatccgta gatgaatcca
     3241 gtttcgctat tttaacatct acgcggtatt tatctttgat aaatttagct attttgcttt
     3301 caatctcata gaaagcatcc tgttcacaac gcacaacatc gggaaactgt ttttctggct
     3361 taacaagaaa acgtaaccga ttgttgagca ccatcatttc tgcaaacaat ttgatggtaa
     3421 acttgccttc ttcaacgtca cttttaaggt taacgatgaa acgctgatcc ttaagcatac
     3481 ccggtttgtt catttcacga atttggagcg agtcattcca atgccgcaac ggaaccgatg
     3541 cgatcatctt ggtacagtct cttatcgtct gaatagaacg agatgcgtta agcgcagtat
     3601 gcatgtttcc accttagccg gtaataactt tatgtcatag cggcgacctt gtcggtttag
     3661 aggctacgag tatgcgtcga tttattaaaa caaggtatga tttacttgac atccatgctt
     3721 caaatctacc ttgtgctcta ataatatgca aattttatgt tagaaaattc cgcttttatt
     3781 atggcacttt tctgccccat ccacacaatc gctcccctat catattgtga aaaagtactt
     3841 ccctttcagt ctcttcggtc atgagatcat cacggctgat gtatagcggt gtagccgcat
     3901 cgcagaaaag cacaccagag gattgaggct taatcacgca accacttatt gtgcaactca
     3961 tgatgaacag tagaagcatc tttgcgccgt aactcatcaa gtgtttcatt tttgacatcc
     4021 actgtgcttt gaagtcgttt cctgtcttcc tgttttgcct tctcttccat tgctcgtcgc
     4081 gccgcatttc cgcccatcgt gtaagcgccg accagaacga aaagaacggc agccagagta
     4141 atcaaagcaa cttttagctt tatcatcagg ctacctagca tattaggcca tccctttctg
     4201 gtgttttctt acctgcgacc aggcaatgaa tcctgccaca acaatagtgg caataccgaa
     4261 gatgatgcgt actgtatccc cactagagat atgaccttgt gctttatcca tagcagcgga
     4321 aacctgcggc ataacatcgg ccagctgcgc cagaccaata cctgctgtaa cagttgcgcc
     4381 tgctgtttct ttagttacag gaacagcctt cacggttttc accggcttaa cgactccagc
     4441 tcgacgcaga ccttcctcaa taacttctgc cgcataccag gtgttcagcg tttttagcgg
     4501 acctcgccca ttctcatggc gaatgattgc ctcaaccaaa ggtcgaagga tgtcgtaatc
     4561 atgcagatcg atgatcatgt ctgcggttac accaacagct ttagacacct cattaatgta
     4621 ggtgtcagtg ttgttttcat tcggcggtgc ccaacgttca ataacttcgc gaatggtatc
     4681 aatacttgag ccgtctttgg cgcgacgttt atcgtggtag gtaattagag tcactgccag
     4741 agcacgaata ccccaaacag ggtctttaaa cgtgcaaaag cgaggttcgt ctggattcgc
     4801 aaccagacct tgccacggtg atcctttatc aagattaccg gggttattat tacgaatgcc
     4861 tctcggagtc ttcatccttg ttctcctgtt attgcagtcc attttttacg ccatatgcgg
     4921 ctaatcctaa aattagtgcg gttataataa acgacgtcac tttagaaata atgcctccaa
     4981 agaagccgct tgagatcgaa tctaacctat taagtagttt atccagattg gaatgttgaa
     5041 tactgtgttg agccggagtc atgtcaccaa aatagttttt caactgctca tgaacctcat
     5101 gaactatctc ctctctgatt tcttttccta atttaccaac aacttctctg gcgacaattt
     5161 tagctattct ctcaacctga tcatgagtga cgcctcctac gtcattagcc atgcttcctc
     5221 catgagcaat ctattt
//
LOCUS       DAZOZD010000269         5230 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOZD010000269 DAZOZD010000000
VERSION     DAZOZD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.272"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..319
                     /gene="gatA"
                     /locus_tag="ACHUPB_003887"
     CDS             <1..319
                     /gene="gatA"
                     /locus_tag="ACHUPB_003887"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0760576.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
     gene            350..634
                     /gene="gatB"
                     /locus_tag="ACHUPB_003888"
     CDS             350..634
                     /gene="gatB"
                     /locus_tag="ACHUPB_003888"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0760577.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            638..1993
                     /locus_tag="ACHUPB_003889"
     CDS             638..1993
                     /locus_tag="ACHUPB_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0760578.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            2041..3081
                     /gene="gatD"
                     /locus_tag="ACHUPB_003890"
     CDS             2041..3081
                     /gene="gatD"
                     /locus_tag="ACHUPB_003890"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0760579.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            3187..3960
                     /locus_tag="ACHUPB_003891"
     CDS             3187..3960
                     /locus_tag="ACHUPB_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0760580.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4042..4941)
                     /gene="yegS"
                     /locus_tag="ACHUPB_003892"
     CDS             complement(4042..4941)
                     /gene="yegS"
                     /locus_tag="ACHUPB_003892"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0760581.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
BASE COUNT         1311 a         1153 c         1341 g         1425 t
ORIGIN      
        1 agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc
       61 gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca
      121 gcaagcggat gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggagaa
      181 tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat
      241 cgtcgagaca ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt
      301 tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa
      361 gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa
      421 agagttgtgt cagaatcata gtattcctgt tgaattaatc cagtgtcggg ttaatgaaat
      481 agaaacctat atggatggtg tgcatttgat atgcaccact gccaaagtgg atcgtagttt
      541 tggcgatatt ccgttagttc acggcatgcc ttttatttct ggtgtcggta tcgaagcatt
      601 acaaaataaa attctgacta tcttacaggg gtgacctatg ttttcagaag tcatgcgtta
      661 tattctcgac ctcggcccta cggtgatgct tccgattgtc atcattattt tttctaaaat
      721 attaggcatg aaggcaggcg attgctttaa agcgggtctg catatcggga ttggctttgt
      781 tggcattggc cttgtgattg gcttaatgct ggattccatt ggtccggcgg cgaaagcgat
      841 ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg gctcttcacc
      901 aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg attgcgattc tggttaacgt
      961 ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga atatctggca
     1021 tatgaccttc accggcgcgt tgctgcatct ggcaaccggt tcatggatga tagggatggc
     1081 aggtgtggta attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac
     1141 ccgaaatttc tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg
     1201 gccgattgcg gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa
     1261 atttagcgcc gacgatattc agcgcaaatt tggtccattt ggcgagcctg tcaccgtggg
     1321 ttttgtgatg gggctgatta tcggcatcct cgccggttac gatgtcaaag gtgtattgca
     1381 gctggcggta aaaacggcgg cagttatgct gctgatgcca cgggtgatta aacccatcat
     1441 ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg
     1501 tcaggagttc ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc
     1561 ggcaagcctg atttttatcc cgctcaccat tttaattgct gtttgtgttc cgggtaatca
     1621 ggtactgccg tttggcgatc ttgccaccat cggcttcttc gtggcgatgg cggtcgcggt
     1681 gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct
     1741 gtggatcgcg acgcaaacta ttggtttgca cacccaactg gcggctaatg ctggggcgtt
     1801 aaaagccggg ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact
     1861 gattcaggtt ttctccacgc aaaatattcc cggtttcatt attatcggcg caatttatct
     1921 taccggtatt ttcatgacct ggcgtagagc gcgtggtttt attaaacaag agaaaaccgt
     1981 tctcgcagaa taatttttac ctgaggggga ggttatcccc cttaacaatc aggagttttt
     2041 atgaaatcaa tggtgaatga tactgatggt atcgtgcgcg ttgcagaaag cgtcattcct
     2101 gaaattaaac atcaggatga ggtgcgggta aaaattgcca gctcgggctt atgtggttcc
     2161 gatttaccca ggatatttaa aaatggtgca cattattatc caataacgtt aggccatgaa
     2221 tttagcggct atattgatgc ggtgggatcc ggtgttgatg atttacaccc tggcgatgcg
     2281 gttgcctgtg tgccgttatt accctgtttt acttatccag agtgtttgaa agggttttat
     2341 tcccagtgcg caaaatatga ttttattggc tcgcggcgtg atggtggatt tgctgaatat
     2401 attgtcgtta agcgaaaaaa tgtctttgct ctacccacgg atatgcctat tgaggatggg
     2461 gcttttattg agccgattac cgttggcctg catgcttttc atttagcgca aggttgtgag
     2521 aataaaaacg ttattattat tggtgccgga accattggcc tgctggctat tcagtgcgct
     2581 gtcgcgctgg gagcaaagag tgtgacggcg atcgacatta gctcagaaaa actggcattg
     2641 gcaaaatctt tcggtgcgat gcaaacattt aacagtagcg aaatgagcgc gccgcaaatg
     2701 cagagcgttt tacgcgaact gcgctttaat cagcttatcc tcgagacggc tggcgtaccg
     2761 caaactgtcg aactggcggt agagattgcc ggtcctcatg cccaactggc gctggtgggc
     2821 acgttgcatc aggatctgca tttaacatcg gcaacgtttg gcaaaatatt gcgtaaagag
     2881 ctgacggtta tcggcagttg gatgaactat tccagccctt ggccggggca ggagtgggaa
     2941 acggcgagcc ggttgctgac agaacgtaag ttaagcctgg agccattaat cgctcaccgt
     3001 ggaagctttg aaagcttcac ccaggcggtg cgtgacatcg ctcgtaatgc catgccgggc
     3061 aaagtgttgc tcattccatg aaaccgcggg ccagcgtgat gctggcccgg tattgtgcaa
     3121 aacagattat tcaccaatgg tcccccttcg tttacactag ccacaattga atatgggtaa
     3181 atgacgatga attcattcga gcgaaggaat aagatcatcc aattagtgaa tgaacaggga
     3241 accgtgcttg ttcaggatct ggcgggagta tttgctgcct cggaagcgac aatccgtgcc
     3301 gatttgcgct ttctcgaaca aaaaggcgtg gttacgcgct ttcatggcgg tgcggcgaaa
     3361 ataatgtccg gtaatagtga aaccgagacc caggaagtcg ggtttaaaga gcgatttcag
     3421 ctcgccagcg cgccaaaaaa cagaatagcg caggcggcag tcaaaatgat ccatgaaggg
     3481 atgaccgtta ttctcgacag cggaagtacg acaatgctta tcgccgaagg attaatgacc
     3541 gctaaaaata tcacggtgat taccaacagt ctcccggcag cgtttgccct ttccgaaaat
     3601 aaagacatta ctctggtcgt ctgtggtggc acggtccgcc ataaaacgcg ctcgatgcat
     3661 ggttccattg ccgagcgttc attgcaggat attaatgccg atttaatgtt tgtcggtgct
     3721 gatggtattg atgcggttaa tggtattacg acctttaatg aaggttattc gattagcggt
     3781 gcgatggtca ccgccgctaa taaagtgatt gccgttctcg attcatcgaa atttaaccgt
     3841 cgcggtttta atcaggtact gccaatcgaa aaaattgaca ttattattac cgatgatgct
     3901 gtatcagagg tggataaact ggcattgcag aagacacggg taaaattaat tacggtgtag
     3961 tattgttctg tatgccggat aaggtattta caccgcatcc ggcatttcgg attctccctg
     4021 gttcaagaga gaatagtttg attaacgcaa taatagacaa tctggtggta atcgacaacg
     4081 caacgccgcc ggaagtattt caatatgaaa gttttgtccg ctcaacggtt cgccatcaag
     4141 attaaaagtg atttcgtgtg gggcttgtat atcaaaccac gacgaagcgc cttcgataat
     4201 attcgggtta tcttcgtcag attttaaggt tgatacgaga gccggaagaa tttcatcgcc
     4261 ggtaaaaatg cgcagttgca gcaagccatc gttaattaac gcgttcggac acaattgctg
     4321 accgccaccg gcctgacgcc cgttaccaat accaatgacc agggcgtcac cttgccagtg
     4381 aaagttttca ccgcggattt cacaacggtc cggttgcaga gtatccatgc gcattaagcc
     4441 atgaatgatg taagagacgc cacccagcgc ggcttttaat ttttccggcg tttctgtggt
     4501 aatacgcgtc ccaaatccgc ctgtcgccat attaataaaa caggtttgtt tgttgacctg
     4561 cgccatatct atcgcaatgg cgttaccggc aattgccagt ttcagcgcct tatccagtgc
     4621 ctcaggaatc cctacactgg tggcaaaatc attggcggtt cctaatggca aaattcccag
     4681 cgcgggtatg ccatccccct cacactgaat caacgccgta gaaacttcat taatggtgcc
     4741 atcgccacca ccggcaatca ccgttgcgac gcccaacttc cgggcctcct ctacatatcg
     4801 tgcggcatcg cctttctccc aggtgacccg cacatggatc gtcattcctt cctcacgcaa
     4861 cagcataatt gcttcgcgca agggtagatt gtcagtactt ttgccattaa gaatcagtaa
     4921 gctggcggga aattctgcca tgatcgtatg tgcctttatg attggtctgt agatagtgta
     4981 gagcagaaaa caaaaggtgg agtcagaaca ggatgaaagt cggaggataa aaagtcagtt
     5041 caagtatgga ggattcagtg gttaccacca ggagcttgca atggggacag cgaatactaa
     5101 cggctgccac cgataaattt caaaaaagag catataccta atattcaact aaacagtggc
     5161 atcttcaata taatatatta aagcccccat ggagttaccc tgaagggcct caatgtccgt
     5221 aattcctact
//
LOCUS       DAZOZD010000270         5219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOZD010000270 DAZOZD010000000
VERSION     DAZOZD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.273"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHUPB_003893"
     CDS             <1..227
                     /locus_tag="ACHUPB_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760582.1"
                     /translation="KNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPA
                     IIHSALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(291..575)
                     /locus_tag="ACHUPB_003894"
     CDS             complement(291..575)
                     /locus_tag="ACHUPB_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="HFR0760583.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="ACHUPB_003895"
     CDS             complement(593..790)
                     /locus_tag="ACHUPB_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760584.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="ACHUPB_003896"
     CDS             1002..1298
                     /locus_tag="ACHUPB_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760585.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="ACHUPB_003897"
     CDS             complement(1843..2076)
                     /locus_tag="ACHUPB_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR0760586.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="ACHUPB_003898"
     CDS             complement(2083..2613)
                     /locus_tag="ACHUPB_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR0760587.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2651..3349)
                     /locus_tag="ACHUPB_003899"
                     /pseudo
     CDS             complement(2651..3349)
                     /locus_tag="ACHUPB_003899"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="ACHUPB_003900"
     CDS             complement(3291..3614)
                     /locus_tag="ACHUPB_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR0760588.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="ACHUPB_003901"
     CDS             3679..3870
                     /locus_tag="ACHUPB_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0760589.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4538..4651
                     /locus_tag="ACHUPB_003902"
     CDS             4538..4651
                     /locus_tag="ACHUPB_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0760590.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
BASE COUNT         1373 a         1427 c         1116 g         1303 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAZOZD010000271         5157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOZD010000271 DAZOZD010000000
VERSION     DAZOZD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.274"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            35..1468
                     /gene="cscA"
                     /locus_tag="ACHUPB_003903"
     CDS             35..1468
                     /gene="cscA"
                     /locus_tag="ACHUPB_003903"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0760591.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1476..2471)
                     /locus_tag="ACHUPB_003904"
     CDS             complement(1476..2471)
                     /locus_tag="ACHUPB_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0760592.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2702..2920
                     /locus_tag="ACHUPB_003905"
                     /pseudo
     CDS             2702..2920
                     /locus_tag="ACHUPB_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2938..4266
                     /gene="dsdA"
                     /locus_tag="ACHUPB_003906"
     CDS             2938..4266
                     /gene="dsdA"
                     /locus_tag="ACHUPB_003906"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0760593.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4374..>5157)
                     /gene="emrY"
                     /locus_tag="ACHUPB_003907"
     CDS             complement(4374..>5157)
                     /gene="emrY"
                     /locus_tag="ACHUPB_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0760594.1"
                     /translation="VIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMP
                     QLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYW
                     RSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFFRTL
                     SGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINN
                     EITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG"
BASE COUNT         1390 a         1268 c         1272 g         1227 t
ORIGIN      
        1 aattcaccaa atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc
       61 gcaaaacgca ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca
      121 cctcgcgcca cctgccggat ggatgaacga tccaaacggc ctgatctggt ttaacgatcg
      181 ttatcacgcg ttttatcaac atcacccgat gagcgaacac tggggaccaa tgcactgggg
      241 acatgccacc agcgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg
      301 agacgataat gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct
      361 ctcacttatc tacaccggac acgtctggct cgattgtgca ggtaatgacg atgcaattcg
      421 cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat
      481 cctcactcca ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga
      541 cacatggtgg atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta
      601 tcgcggcagt tcattgcgtg aatggacctt cgatcgcgta ctggcccacg ctgatgcggg
      661 tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat
      721 gttttccccg cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg
      781 cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga
      841 acttgataac gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg
      901 tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggatg
      961 ggcaggctgc atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg
     1021 cccggttcac gaagctgagt cgttacgcca gcagcatcaa tctgtctctc cccgcacaat
     1081 cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc
     1141 gctgaagaac agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta
     1201 tattgataac caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga
     1261 cggctaccgt agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga
     1321 tacatcatcc gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta
     1381 tccgcagcca gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca
     1441 acatggagca ctctggctac tgggttaaca taatatcagg tggaacaacg gatcaacagc
     1501 ggacaaggga tccgcgtcac tcttccccct tcacgacctt caataatatg caatgcagct
     1561 tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc
     1621 ccgacgccaa ccagattatc aaagcccatt acggcgacat cctgcgggat acgtaccccc
     1681 ttcgccagga gaacctgata agcgacaaag gctgcacgat cgttaccaca tatcagaaca
     1741 tcaaaatctg gtttgcccga tttgaagtgg gcattgagta aacttgcgag atcggtgtag
     1801 tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca
     1861 cgccaggcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc
     1921 cagaagcata acggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt
     1981 gcagtgtaat catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc
     2041 aatacaatat tttcaccata cagagactca ggcagcgtga tatgtcgcag ccccattgta
     2101 gtatagataa tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca
     2161 tcttcagaaa aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca
     2221 atggcaagca gaatatcaac agagaaagga gtggtagccg tgtcctgcgc cagcacggcg
     2281 agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa
     2341 ttcagggtct ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca
     2401 ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt
     2461 aatgaagcca tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca
     2521 ctggcgagat ccaacgccct atcacctgac acagcaatac aataaaaaat aacaataatt
     2581 cccggacaat tgtccccaat tccgcctctg ttctcgccaa cgagtaacca tataggcaag
     2641 taagtaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat
     2701 tgtgctgccg ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg
     2761 tgcaattggc tgcacgatcg ttacggactc gcttttctgg ctagtgaagc aatattgcgg
     2821 cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt
     2881 cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg
     2941 gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct
     3001 cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat
     3061 gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc
     3121 tatctggcaa aagcatttgc tgaaactgct gccactgggg ggattattga atcagaactg
     3181 gttgccattc cagctatgca aaaacggctg gaaaaagaat atcagcaacc gatcagcggg
     3241 caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc
     3301 gggatttatg aagtcctggc acacgcagaa aaactggctc tggaagcggg gttgctgacg
     3361 cttgatgatg actacagcaa actgctttct ccggagttta aacagttctt tagccaatac
     3421 agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc
     3481 attggcttta aggtgacagt tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa
     3541 ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag
     3601 gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc
     3661 cgcacgttgt tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag
     3721 caaggccgta tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc
     3781 ggtggtcctg gtggcgtcgc attcgggctt aaactggcgt ttggcgatca tgttcactgc
     3841 ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac
     3901 gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca
     3961 gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat
     4021 acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt
     4081 cgtcttgaac cttcggcact ggcgggtatg gccggacctc agcgcgtatg tgcatcagta
     4141 agttaccaac agatgcacgg tttcagcgca gaacaactgc gtaataccac tcatctggtg
     4201 tgggcgacgg gaggtggaat ggtgccggaa gaagagatga atcaatatct ggcaaaaggc
     4261 cgttaataac gtttcaacgc agcatcgcaa tcctttccct gggtgagcga tgctgccgat
     4321 ggcgcagact taagatcccc ggtcttaccc gctataaccc cctcctttta tcatcaccca
     4381 acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa
     4441 gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg ctgggttatt
     4501 tcattattaa tttcattaag aactcctgaa agcgaaccat aatatttatc cataatttgt
     4561 gacgatgaat taaacacggg gttaaattga tcgatggttg ctgtcaactg actatggtgt
     4621 aacgattcgc gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag
     4681 gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa
     4741 aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg aaaaaactgc
     4801 ggcaaaatga tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag
     4861 caaaccgcat acatcaaaaa actaaatgtc actaacaacc gcatgtctat tttgttgcca
     4921 taacgtccta tcaaaggtga aattaatagt ggcatgatgc cgatgggcgc ataagcaagt
     4981 ccggcccata tcgcattata ccccatcgtt tcctggagta actgcggcat aaggacgatc
     5041 gctccagagt aaaataaata cgcgcatgtg atactcacaa taccaatggt gaagttacgg
     5101 gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgactccca aatgact
//
LOCUS       DAZOZD010000272         5140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOZD010000272 DAZOZD010000000
VERSION     DAZOZD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.275"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            16..783
                     /gene="hdhA"
                     /locus_tag="ACHUPB_003908"
     CDS             16..783
                     /gene="hdhA"
                     /locus_tag="ACHUPB_003908"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0760595.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            1004..1594
                     /gene="uidR"
                     /locus_tag="ACHUPB_003909"
     CDS             1004..1594
                     /gene="uidR"
                     /locus_tag="ACHUPB_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0760596.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1983..3794
                     /gene="uidA"
                     /locus_tag="ACHUPB_003910"
     CDS             1983..3794
                     /gene="uidA"
                     /locus_tag="ACHUPB_003910"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0760597.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3791..>5140
                     /gene="uidB"
                     /locus_tag="ACHUPB_003911"
     CDS             3791..>5140
                     /gene="uidB"
                     /locus_tag="ACHUPB_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR0760598.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     "
BASE COUNT         1253 a         1221 c         1356 g         1310 t
ORIGIN      
        1 gacggaaaat gcgccatgcg ccatgcgcca tgcgccatgc gccatgcgcc atgcgccatc
       61 atcacaggtg cgggtgcagg tattggtaaa gaaatcgcca ttacattcgc gacagctggc
      121 gcatctgtgg tggtcagtga tattaacgcc gacgcagcta accatgttgt agacgaaatt
      181 caacaactgg gtggtcaggc atttgcctgc cgttgtgata ttacttccga acaggaactc
      241 tctgcactgg cagactttgc tatcagtaag ctgggtaaag ttgatattct ggttaacaac
      301 gccggtggcg gtggacctaa accgtttgat atgccaatgg cggattttcg ccgtgcttat
      361 gaactgaatg tgttttcttt tttccatctg tcacaacttg ttgcgccaga aatggaaaaa
      421 aatggcggtg gcgttattct gaccatcact tctatggcgg cagaaaataa aaatataaac
      481 atgacttcct atgcatcatc taaagctgca gccagtcatc tggtcagaaa tatggcgttt
      541 gacctgggtg aaaaaaatat tcgggtaaat ggcattgcgc cgggggcaat attaaccgat
      601 gccctgaaat ccgttattac accagaaatt gaacaaaaaa tgttacagca cacgccgatc
      661 agacgtctgg gccaaccgca agatattgct aacgcagcgc tgttcctttg ctcgcctgct
      721 gcgagctggg taagcggaca aattctcacc gtctccggtg gtggggtaca ggagctcaat
      781 taatacacta acggaccggt aaacaaccgt gcgtgttgtt taccgggata aactcatcaa
      841 cgtctctgct aaataactgg cagccaaatc acggctattg gttaaccaat ttcagagtga
      901 aaagtatacg aatagagtgt gccttcgcac tattcaacag caatgatagg cgctcacctg
      961 acaacgcggt aaactagtta ttcacgctaa ctataatggt ttaatgatgg ataacatgca
     1021 gactgaagca caaccgacac ggacccggat cctcaatgct gccagagaga ttttttcaga
     1081 aaatggattt cacagtgcct cgatgaaagc catctgtaaa tcttgcgcca ttagtcccgg
     1141 gacgctctat caccatttca tctccaaaga agccttgatt caggcgatta tcttacagga
     1201 ccaggagagg gcgctggctc gtttccggga accgattaaa gggattcatt ttgttgacta
     1261 tatggtcgag tctattgtct ctctcaccca tgaagccttt gggcaacggg cgctggtggt
     1321 cgaaattatg gcggaaggga tgcgtaaccc acaggtcgcc gccatgctta aaaataagca
     1381 tatgacgatc acggaatttg ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat
     1441 aagcccagac atcaacacgt caatgacttc acgtttactg ctggatctga cctacggtgt
     1501 actggccgat atcgaagcgg aagatctggc gcgtgaagcg tcgtttgctc agggattacg
     1561 cgcgatgatt ggcggtatct taaccgcatc ctgatcctct catttcgcgg gctggtgata
     1621 actgtgcccg cgtttcatac tttaatttcc ctgtacaaaa tacaatctag ccgggatcgg
     1681 agaattcccc cccaaaaata ttcaccgtag ccatatgtca tgagagttta tcgttcccaa
     1741 tatgctcgaa cgaacgttcg gttgcttatt ttatggcttc tgtcaacgcc gttttaaaga
     1801 ttaatgcgat caatatcacg ctgtgggtat tgcagttttt ggttttttga tcgcggtgtc
     1861 agttcttttt atttccattt ctcttccatg ggtttctcac agataactgt gtgcaacaca
     1921 gaattggtta actaatcaga ttaaaggttg accagtattt ttatcttaat gaggagtccc
     1981 ttatgttacg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg
     2041 cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac
     2101 aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc gatgcagata
     2161 ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg
     2221 caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca
     2281 ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc
     2341 cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac gaactgaact
     2401 ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt
     2461 acttccatga tttctttaac tatgccggga tccatcgcag cgtaatgctc tacaccacgc
     2521 cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg
     2581 cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg
     2641 atcaacaggt ggttgcaact ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc
     2701 acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc aaaagccaga
     2761 cagagtgtga tatctacccg cttcgcgtcg gcatccggtc agtggcagtg aagggcgaac
     2821 agttcctgat taaccacaaa ccgttctact ttactggctt tggtcgtcat gaagatgcgg
     2881 acttgcgtgg caaaggattc gataacgtgc tgatggtgca cgaccacgca ttaatggact
     2941 ggattggggc caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact
     3001 gggcagatga acatggcatc gtggtgattg atgaaactgc tgctgtcggc tttaacctct
     3061 ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggcag
     3121 tcaacgggga aactcagcaa gcgcacttac aggcgattaa agagctgata gcgcgtgaca
     3181 aaaaccaccc aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag
     3241 gtgcacggga atatttcgcg ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc
     3301 cgatcacctg cgtcaatgta atgttctgcg acgctcacac cgataccatc agcgatctct
     3361 ttgatgtgct gtgcctgaac cgttattacg gatggtatgt ccaaagcggc gatttggaaa
     3421 cggcagagaa ggtactggaa aaagaacttc tggcctggca ggagaaactg catcagccga
     3481 ttatcatcac cgaatacggc gtggatacgt tagccgggct gcactcaatg tacaccgaca
     3541 tgtggagtga agagtatcag tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg
     3601 tcagcgccgt cgtcggtgaa caggtatgga atttcgccga ttttgcgacc tcgcaaggca
     3661 tattgcgcgt tggcggtaac aagaagggga tcttcacccg cgaccgcaaa ccgaagtcgg
     3721 cggcttttct gctgcaaaaa cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg
     3781 gaggcaaaca atgaatcaac aactctcctg gcgcaccatc gtcggctaca gcctcggtga
     3841 cgtcgccaat aacttcgcct tcgcaatggg ggtgctcttc ctgttgagtt actacaccga
     3901 cgtcgctggc gtcggtgccg ctgcggcggg caccatgctg ttactggtgc gggtattcga
     3961 tgccttcgcc gacgtctttg ccggacgagt ggtggacagt gtgaataccc gctggggaaa
     4021 attccgcccg tttttactct tcggtactgc gccgttaatg atcttcagcg tgctggtatt
     4081 ctgggtaccg accgactgga gccatagccg caaagtagtg tatgcatatt tgacctacat
     4141 gggcctcggg ttttgctaca gcctggtgaa tattccttat ggttcacttg ctaccgcgat
     4201 gacccaacaa ccacaatccc gcgcccgtct gggcgcggct cgtgggattg ccgcttcatt
     4261 gacctttgtc tgcctggcat ttctgatagg gccgagcatt aagaactcca gcccggaaga
     4321 gatggtgtcg gtataccatt tctggacgat tgtgctggcg attgccggaa tggtgcttta
     4381 cttcatctgc ttcaaatcga cgcgtgagaa tgtggtacgt atcgttgcgc agccgtcatt
     4441 gaagatcagt ttgcaaaccc tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc
     4501 gctgtgtgtg ctgatttcga cctttgcggt cagcgcctcg tcgttgttct acgtgcgcta
     4561 tgtgttaaat gataccgggc tgttcactgt gctggtactg gtgcaaaacc tggttggtac
     4621 tgtggcatcg gcaccgcttg tgccggggat ggtcgcgagg atcggtaaaa agaatacctt
     4681 cctgattggc gctttgctgg gaacctgcgg ttatctgcta ttcttctggg tttccgtctg
     4741 gtcactgccg gtggcgttgg ttgcgttggc catcgcttca attggtcagg gcgttactat
     4801 gacagtgatg tgggcactgg aagctgatac cgtagaatac ggtgaatacc tgaccggcgt
     4861 gcgaattgaa gggctcacct attcactatt ctcatttacc cgtaaatgcg gtcaggcaat
     4921 cggaggttca attcctgcct ttattttggg gttaagcgga tatattgcca atcaggtgca
     4981 aacgccggaa gttattatgg gcatccgcac atcaattgcc ttagtacctt gcggatttat
     5041 gctactggca ttcgttatta tctggtttta tccgcttacg gataaaaaat tcaaagaaat
     5101 cgtggttgaa attgataatc gtaaaaaagt gcagcagcaa
//
LOCUS       DAZOZD010000273         5114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOZD010000273 DAZOZD010000000
VERSION     DAZOZD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.276"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHUPB_003912"
     CDS             <1..379
                     /locus_tag="ACHUPB_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760599.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="ACHUPB_003913"
     CDS             complement(575..2125)
                     /locus_tag="ACHUPB_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR0760600.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="ACHUPB_003914"
     CDS             2297..2743
                     /locus_tag="ACHUPB_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR0760601.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="ACHUPB_003915"
     CDS             complement(2937..3872)
                     /locus_tag="ACHUPB_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0760602.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4393..>5114
                     /locus_tag="ACHUPB_003916"
     CDS             4393..>5114
                     /locus_tag="ACHUPB_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760603.1"
                     /translation="MNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMTVGK
                     AFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSIAQN
                     NNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYLDRM
                     LYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKNASP
                     LITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAZOZD010000274         5044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOZD010000274 DAZOZD010000000
VERSION     DAZOZD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.277"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUPB_003917"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="ACHUPB_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1771
                     /locus_tag="ACHUPB_003918"
                     /pseudo
     CDS             1595..1771
                     /locus_tag="ACHUPB_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1779..1868)
                     /locus_tag="ACHUPB_003919"
                     /pseudo
     CDS             complement(1779..1868)
                     /locus_tag="ACHUPB_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUPB_003920"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHUPB_003920"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0760604.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="ACHUPB_003921"
     CDS             complement(3750..4700)
                     /locus_tag="ACHUPB_003921"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0760605.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="ACHUPB_003922"
     CDS             complement(4839..>5044)
                     /locus_tag="ACHUPB_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0760606.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAZOZD010000275         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOZD010000275 DAZOZD010000000
VERSION     DAZOZD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.278"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..677)
                     /locus_tag="ACHUPB_003923"
     CDS             complement(<1..677)
                     /locus_tag="ACHUPB_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0760607.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPMESLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREA"
     gene            906..1514
                     /locus_tag="ACHUPB_003924"
     CDS             906..1514
                     /locus_tag="ACHUPB_003924"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0760608.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1612..3003
                     /gene="selA"
                     /locus_tag="ACHUPB_003925"
     CDS             1612..3003
                     /gene="selA"
                     /locus_tag="ACHUPB_003925"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0760609.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3000..4844
                     /gene="selB"
                     /locus_tag="ACHUPB_003926"
     CDS             3000..4844
                     /gene="selB"
                     /locus_tag="ACHUPB_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0760610.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1068 a         1279 c         1443 g         1080 t
ORIGIN      
        1 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
       61 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      121 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      181 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      241 acgccgccgc gcaccagcca gagtgattcg ctgcggctgt aaccgtcctc accgggaaac
      301 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      361 agctgtaagc ggatatccgc aggcattttc cagccggggc cgaggctctc catcggcgcg
      421 ggcgtttttg tccggtaact gctgtaggtg cgggagagga tgaacggcag cggggcgggc
      481 agggcgatgt cggtttcacc gggaaggacc tttgcaccga gcaggggatt gaccggatgg
      541 ccggacgtca ctccgccggg gcacaccgaa caggccacac cggtgggggc accgatgcgc
      601 accccggctg aaccctgaac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgct
      661 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
      721 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
      781 ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac aaaccatcaa
      841 cattttctgc cctttttccc atcaccaggc catactgata tttttcctcg cacaaaggac
      901 tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt tctattctgt
      961 tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac gcggacaacg
     1021 gcgtggcgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa gagggcgaat
     1081 gctggtttga ttcgccgatc atcgctgaat atattgagtt aatgaatgtc gctccggcga
     1141 tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggcg ctggcggatg
     1201 gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccggcg gcgcagcagt
     1261 ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag
     1321 gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact atcgccattg
     1381 cctgtgctgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc
     1441 cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac
     1501 cgccaaaggc ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca atccgtggtt
     1561 atgttaaacg cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc
     1621 gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc
     1681 ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg
     1741 ctcgacgaag cgcgagaagt gattcgtgac agccagacgc ttcctgcgtg gtgtgaaaac
     1801 tgggcgcaag aagtcgatgc ccggttgacg aaagaggcgc agagcgcgct gcgtccggtg
     1861 atcaacctga cgggaaccgt gctgcatacc aaccttggac gagctttaca ggcggaagcc
     1921 gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta cgatctggac
     1981 gacgccggac gcggacatcg cgatcgggcg ctggcgcaac tgctgtgccg tattacgggg
     2041 gcggaagatg cctgtatcgt caataacaat gcggcggcgg tgttattgat gttggcggcc
     2101 actgccagcg ggaaagaggt ggtagtatct cgcggcgaat tggtggagat tggcggcgcg
     2161 tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt agggaccacc
     2221 aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc actgttgatg
     2281 aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga tgaagcggaa
     2341 ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg
     2401 ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg
     2461 gcgggcgtca gtctggtgag cttctccggc gacaagttgt taggcgggcc gcaggcaggg
     2521 attatcgttg gtaaaaaaga gatgatcgcc cgcctgcaaa gccacccgct gaagcgtgca
     2581 ttacgcgcgg ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct ttatttacac
     2641 cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc
     2701 attcaaatcc aggcacaacg tttacaggct ccccttgccg cacattacgg cgcggagttt
     2761 gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc
     2821 ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca
     2881 ttagccgccc gctggcgtga attgccagtg ccgatgattg gtcgtattta tgacggacga
     2941 ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat gttgttgaaa
     3001 tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg caggtgatta
     3061 ctggcgtaaa tgctgaccgt ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg
     3121 gctatgccta ctggccgcag ccggatggtc gcgtgcctag ttttatcgac gtacccggtc
     3181 atgaaaagtt tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg
     3241 tggtggcgtg cgatgacggc gtgatggcgc agacccgtga gcatctggcg attttgcagc
     3301 tgaccggtaa cccgatgctg acagtggcgc tgaccaaagc cgatcgcgtg gacgaagcgc
     3361 gtgttgatga ggttgaacgc caggtaaagg aggtgctgcg ggaatacggt tttgctgagg
     3421 caaaactgtt tatcaccgca gcaaccgaag gtcggggaat tgatgccctg cgcgagcatc
     3481 tgcttcagtt accaggacgt gaacacgcca gccaacatag tgtccgcctc gcgatcgacc
     3541 gcgcatttac cgtaaaaggt gccggtctgg tcgtcaccgg tacggcgtta agcggggaag
     3601 tgaaggtagg cgattcactc tggctgactg gtgtaaataa accgatgcgt gtacgtgcgc
     3661 tgcatgcaca aaaccagcca acagaaaccg cccatgccgg gcagcgtatc gcgcttaaca
     3721 tcgcgggtga tgcggaaaaa gagcagatta accgtggcga ctggctgctt gccgatgcgc
     3781 cgccagagcc gttcacacgg gtgattgtcg agcttcaaac ccatacgccg ctgacccagt
     3841 ggcagccgct gcatattcac cacgccgcca gccacgttac gggacgcgtt tcgctgctgg
     3901 aagataacct tgccgagctg gtgttcgaca cgccgctgtg gctggcggat aacgatcgct
     3961 tagtgctgcg cgatatctcg gcccgcaaca cgctggcggg ggcgcgtgtg gtgatgctta
     4021 acccgccgcg tcgcggtaaa cgtaagccgg aatatctgca atggctggcg tctcttgcac
     4081 gggcgcagag cgatgccgat gcgttatctg ttcatctgga acgcggcgcg gttaaccttg
     4141 cggatttcgc ctgggcgcgc cagctcaacg gcgaagggat gcgcgaattg ctgcaacagc
     4201 ctggctatat tcaggctggt tatagcttgt tgaatgcgcc ggttgccgcc cgctggcagc
     4261 ggaaaattct cgacacatta gcgacttatc atgagcaaca tcgcgatgaa cctggtcctg
     4321 ggcgcgaacg tctgcgacgt atggcgttgc caatggaaga tgaagcgctg gtactgttgc
     4381 tgattgaaaa gatgcgcgaa agcggcgaca tcctcagtca tcacggttgg ctgcatctgc
     4441 cggatcacaa agcgggcttc agcgaagagc agcaggccat ctggcaaaaa gcagagccgc
     4501 tgtttggtga cgagccgtgg tgggtgcgtg atctggcaaa agagacgggt accgacgagc
     4561 aggcaatgcg cctgacgcta cgccaggcgg cgcagcaagg aataattacc gcgatcgtca
     4621 aagatcgtta ttaccgtaac gatcggattg tcgaatttgc caatatgatc cgcgatctcg
     4681 atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa
     4741 agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt cgtcgtggaa
     4801 atgatcattt attgcgcgac gcattattat ttccggaaaa ataaggaaat gattaattaa
     4861 tttttaaaat
//
LOCUS       DAZOZD010000276         4860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOZD010000276 DAZOZD010000000
VERSION     DAZOZD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.279"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUPB_003927"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUPB_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(796..1248)
                     /locus_tag="ACHUPB_003928"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUPB_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUPB_003929"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUPB_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUPB_003930"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUPB_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0760611.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUPB_003931"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUPB_003931"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUPB_003932"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUPB_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0760612.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..>4860
                     /gene="mlaA"
                     /locus_tag="ACHUPB_003933"
     CDS             4153..>4860
                     /gene="mlaA"
                     /locus_tag="ACHUPB_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0760613.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENP"
BASE COUNT         1157 a         1138 c         1145 g         1420 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
//
LOCUS       DAZOZD010000277         4853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOZD010000277 DAZOZD010000000
VERSION     DAZOZD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.280"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1249
                     /locus_tag="ACHUPB_003934"
     CDS             <1..1249
                     /locus_tag="ACHUPB_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFR0760614.1"
                     /translation="YRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVL
                     DSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMA
                     SPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLD
                     EMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYI
                     ENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVD
                     FDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQ
                     WFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWK
                     KSGPEMASLEAWLEEHEKYRSVA"
     gene            1246..1632
                     /gene="trbI"
                     /locus_tag="ACHUPB_003935"
     CDS             1246..1632
                     /gene="trbI"
                     /locus_tag="ACHUPB_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0760615.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            1629..1940
                     /locus_tag="ACHUPB_003936"
                     /pseudo
     CDS             1629..1940
                     /locus_tag="ACHUPB_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            1935..2933
                     /locus_tag="ACHUPB_003937"
                     /pseudo
     CDS             1935..2933
                     /locus_tag="ACHUPB_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            2953..3700
                     /gene="traX"
                     /locus_tag="ACHUPB_003938"
                     /pseudo
     CDS             2953..3700
                     /gene="traX"
                     /locus_tag="ACHUPB_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            3755..4315
                     /locus_tag="ACHUPB_003939"
     CDS             3755..4315
                     /locus_tag="ACHUPB_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0760616.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            4445..4657
                     /locus_tag="ACHUPB_003940"
     CDS             4445..4657
                     /locus_tag="ACHUPB_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0760617.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1132 a         1213 c         1534 g          974 t
ORIGIN      
        1 ctaccgtaac cagctggcgt ttatcgatat tttcttccgg ggaatgaata acaccaacta
       61 caacatggcg gtctgtggca cctccggggc cggtaaaacc gggctgatac agccacttat
      121 ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc gacatggggg atggctacaa
      181 gtccctgtgt gagaacatgg gtggcgtata tctggacggt gaaaccctgc gttttaaccc
      241 gtttgcgaac atcaccgata tcgaccagtc agcggagcgt gtccgtgacc agttgtcagt
      301 gatggccagc cccaacggta acctggatga agtgcatgaa ggtctgctgc tgcaggcggt
      361 cagggcctcc tggctggcca aagagaacag ggcacgtatt gatgacgtgg tggatttcct
      421 gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg actatccgca gccgtctgga
      481 cgaaatgatt gtgctgcttg accagtacac tgccaacggc acttacggcc agtattttaa
      541 ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg gtgctggagc tgggcggact
      601 ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc ctgattatct acatcgagaa
      661 caggatgtac cgcacgccgc gtaacctcaa gaaactgaac gtcattgatg aaggctggcg
      721 tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt gagaaaggtt accgtaccgc
      781 ccgtcgtcat accggtgctt atatcaccat cacacagaac atcgtcgact ttgactctga
      841 caaggcctcc agtgcggccc gtgcggcatg gggtaactcc tcctacaaaa ttatcctcaa
      901 acagagtgcg aaggagttcg caaaatacaa ccagctgtat ccggaccagt tccagccact
      961 gcagcgcgac atgattggta agtttggtgc ggccaaagac cagtggttca gttccttcct
     1021 gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt gtggacccgt taagccgcgc
     1081 catgtacagc tctgacggcc cggattttga gtttgtgcag cagaaacgta aagaggggct
     1141 gagtattcat gaggcggtgt ggcagctggc gtggaagaag tcagggccgg agatggcttc
     1201 gctggaagcc tggctggaag aacatgagaa atacaggagt gttgcatgac cacaacgcaa
     1261 aaaacgactg atgttacagc cccccgacgc agccactggt ggtggacggt gccgggatgc
     1321 ctggcaatgg tgttactgaa cgcagccgtc agttacggca ttgtcagact gaacgcaccg
     1381 atgactgccg ccttcaacat gaaacagact gtggatgcat tttttgacag cgccagtcag
     1441 aaacaactgt cagaagcaca gtcaaaagcc ctttcagcgc gttttaacac ggcactggaa
     1501 gccagcctgc aggcgtggca gcagaaacac cacgcggtca ttctggtgtc gcctgccgtg
     1561 gtacaggggg ctccggatat cacccgggaa atccagcagg atattgcccg gcgaatgaga
     1621 gcggaaccat gagatgccgg gggctgattg ccctgctgat atgggggcag agtgtggccg
     1681 ccgccgatct tggcacctgg ggtgatctgt ggccggtaaa ggagccggac atgctgacgg
     1741 tgatcatgca gcgcctgacg gcactggagc agtccggtga gatgggccgg aaaatggatg
     1801 cctttaagga gcgggtgatc cgcaacagtc tgcggccccc tgccgtgccc ggtatcggac
     1861 gcacggagaa atacagcagc cggttgtttg acccgtccgt cagactggct gcggatatcc
     1921 gggataacga agggcgggtg gccggctttg agtcagccta cgtggccctg tcgcgtatga
     1981 agcagcatgt gcaggtgtac accgataacc gtcagggctg gacggatgcc atcaacaatg
     2041 ccgtacagaa aggaactgcc cacgatgtgt ttgagccgaa accggaccgg gaggtcatga
     2101 atgcagagcg gctgttcagt acggcgcggg agctgcggga cgtggcggca ggccgtgccg
     2161 ttctccgtca ggcggggctg gccgggggag acagtcctgc acggtttatt gctccgggac
     2221 gtaaatatcc gcaaccgtat gtggcactgc cggcgtttga ccgtaacggc aagtccgccg
     2281 gtatctggct gaacccgctg accacggatg acggaaacgg gctgcgggga ttcagcggtg
     2341 aaggtcgggt aaaaggcagc ggggatgcgc agttcgtggc cctgcagggc agccgtaacg
     2401 gagaaagcct gctggctgat aatatgcagg atggcgtccg gattgcccgt gataatcctg
     2461 acagtggtgt ggtggtaagc attgccggtg aaggccgtcc gtggaatccc ggtgccatca
     2521 ccgatggccg cgtgtggggg gatatcccgg acaacagcgt ccagccggga gccggaaatg
     2581 gcgaaccggt cacggcagag gtactggcac agcggcaggc tgaagaggcc atccgccgtg
     2641 aaacggaacg tcgcgcagat gaaattgtcc gtaaaatggc agagaacaaa cctgacctgc
     2701 cggacggcaa aacagagctg gctgtcaggg aaattgccgg gcaggagcgt gaccgggctg
     2761 ccataactga acgggaagcc gcgctgccgg agagtgtgct gcgtgaatca caacgggagc
     2821 gggaagcggt ccgggaggtt gcccgggaaa atctgctgca ggagcgtctg cagcagatgg
     2881 agcgggatat ggttcgtgac ctgcagaaag agaaaacccc gggcggagac tgatacagga
     2941 agacaaacac tgatgacaac ggataacacg aacacgacac gtaacgattc actggctgca
     3001 cggaccgata cctggttgca gtcatttctg gtctggtcac ccggacagcg ggatatcatc
     3061 aaaacggtgg cactggtgct gatggtactg gaccacatca atctgatatt ccagctgaag
     3121 caggaatgga tgtttctggc cgggcgagga gcctttccgc tttttgccct ggtgtggggg
     3181 ctgaatctgt cccgtcatgc gcatatccgg caaccggcca ttaaccggct gtggggatgg
     3241 ggaattattg cccagttcgc atattatctg gcaggctttc cctggtatga ggggaatatc
     3301 ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt gtgaaacgcg cagtgggtgg
     3361 cgtacagcag cggccattct tctgatggcc ctgttggggg cctttgtccg gcaccagtta
     3421 cggcattgcc gggctgctga tgctggcagt cagccaccga ctgtaccggg cggaagacag
     3481 agcggaacgt ctggcgctgg tcgcctgcct gctggctgtt attccggcgc ttaaccttgc
     3541 caccagtgat gcagcggcgg tggccggtct tgtgatgacg gtgctgaccg ttggtctggt
     3601 gtcgtgtgca ggaaaatcat taccccgttt ctggcccggg gattttttcc cggtattcta
     3661 cgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga cgggtgtggt atctttgacc
     3721 gtaacaggat gattcgtcag aggcagaaca cagcatgaca gagcagaagc gaccggtact
     3781 gacactgaag cggaaaacgg aaggggagac gcctgtccgc agccggaaaa ccatcatcaa
     3841 tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag ctggccgaga aagcagcccg
     3901 ggaagcagag ctggcggcaa aaaaagcgca ggccagacag gcactgtcca tttatctgaa
     3961 cctgcccacg ctggatgagg ccgtgaatac cctgaaaccc tggtggccgg gattatttga
     4021 cggtgacacc ccccggcttc tggcctgcgg tatccgggac gtgttactgg aagacgtggc
     4081 gcagcggaat atcccgctct cgcataaaaa actgcgcagg gcgctgaagg ccatcacccg
     4141 ttcagaaagc tatctgtgtg ccatgaaagc cggtgcctgc cggtatgaca cggaagggta
     4201 tgtgacggag catatttctc aggaggaaga agcgtatgcg gcagcgcgtc tggataaaat
     4261 ccgccgccag aaccggataa aggcagaact tcaggccgtg cttgatgaga aataaaaaaa
     4321 agcctccatc agagaggagg cagggaaata aggcttaaaa ggaaataact tccgcttccg
     4381 aaaggaagcc agtccactga attatttatc acaataatac tgacaactaa agggggacaa
     4441 gaaaatgaat ggattcagaa acagttcacg gaacggccag gtctggcgtt accagcgtgc
     4501 cggcgggcgg gccgttattc tggaagtcag tggacgctgg atggaagcgg cagaagcatg
     4561 gcgacgggct gcctgtgttg cgcccaggac agactggcag cagtttgccc gaaaaagggc
     4621 tgaacactgc caccggcgct gccggggaag ggtgtaacga aaaaagcagc cttcattact
     4681 ccaggagtca gaaggctgaa aggagtttat gagatttgct ttttttaata tccggtcggg
     4741 cagtatgacc ggacagttct gagtagcaca gagttttttc cgtattaagt cgtgatttta
     4801 tattttgtga ttaatttcac aaaataaggt gttattcagt gtgtgctgcg ata
//
LOCUS       DAZOZD010000278         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOZD010000278 DAZOZD010000000
VERSION     DAZOZD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.281"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(44..2113)
                     /gene="glyS"
                     /locus_tag="ACHUPB_003941"
     CDS             complement(44..2113)
                     /gene="glyS"
                     /locus_tag="ACHUPB_003941"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0760618.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2123..3034)
                     /gene="glyQ"
                     /locus_tag="ACHUPB_003942"
     CDS             complement(2123..3034)
                     /gene="glyQ"
                     /locus_tag="ACHUPB_003942"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0760619.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3129..3428)
                     /locus_tag="ACHUPB_003943"
     CDS             complement(3129..3428)
                     /locus_tag="ACHUPB_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0760620.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3603..4598
                     /gene="wecH"
                     /locus_tag="ACHUPB_003944"
     CDS             3603..4598
                     /gene="wecH"
                     /locus_tag="ACHUPB_003944"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0760621.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4640..>4803)
                     /locus_tag="ACHUPB_003945"
     CDS             complement(4640..>4803)
                     /locus_tag="ACHUPB_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0760622.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1095 a         1265 c         1221 g         1222 t
ORIGIN      
        1 aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc aacagcgaaa
       61 tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga cggttgagac
      121 gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca accggctcac
      181 gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag tacggctcca
      241 gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc tctttcaggg
      301 tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccagaatg ttagatacac
      361 gtttgttcgc cgccgccagt gcagcagctg catccagggt acggaaatgc gataccgctt
      421 tcatacgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc gcctggatgg
      481 tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga
      541 taacatcatc aactacgttg gcgttagtca gcttatcgcc atacagacgc actgcttctt
      601 cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc agcacgccaa
      661 gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctga ccgataccga
      721 agatacccgc cagagtatcc atcttatcag caatcgccag cgcacaagct accgggttgg
      781 acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcg acggctacgt
      841 cttccgcttc gccatcgtga cgcgcgtagt gcatccccat aacgccttgg gtgtcggtga
      901 actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca cgggttgcgt
      961 ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg atgcggtcag
     1021 ttttgtcacg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg cgcggcaggt
     1081 tatcttccag acgtttttta cggtcggtat tgaagaagaa ctcggcatcc gccagacgtg
     1141 gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat tcgatgttgg
     1201 caacgaagat gaagttcggc agcagtttgc catcgttcgc atacaccggg aagtatttct
     1261 ggtcaccttt catggtgtaa accagggctt cagccggcac cgcgaggaat ttctcttcga
     1321 attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc agcaggcttt
     1381 cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg gctttaatct
     1441 tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga atttccggat
     1501 actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg ccgcgaatca
     1561 cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc agcagcaggg
     1621 tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc atcagtttcg
     1681 ggatcggcag tttcgccagc gaagtcgcaa ccatattcgg cagtagtgct tcagtgcttt
     1741 cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc agacgctcag
     1801 cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg ctcggtttgc
     1861 cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca cgatctggtt
     1921 gcgcttccgc caggttagct actttcaacg ccagacgacg cggagcagca aaccattgaa
     1981 cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaagttcgca gcaaaggact
     2041 cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc accagaaaag
     2101 ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag ccgagggctt
     2161 cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt
     2221 agcgctgacg ctcagtgacg gagatggctt tacgcgcatc cagcaggttg aagctgtggg
     2281 cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc gccagcagct
     2341 gctgcgcttc tttctcgtac tgctcgaagc aggtgaacag gaaatccaca tccgcgtatt
     2401 cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg taggtggttt
     2461 tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca
     2521 tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac tccagaccac
     2581 caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac acttcccagc
     2641 ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt
     2701 gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc tgaatattgt
     2761 ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg ttggggtttt
     2821 cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc gccatcggtt
     2881 ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg acttccatgt
     2941 ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag gtcaggatca
     3001 agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga tacgtgtgga
     3061 tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga aacgggaaaa
     3121 agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca
     3181 aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa ctgttcgaag
     3241 gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg atccttgcat
     3301 aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc tttctgcacg
     3361 ggtgacggtg tagaccaacc cactagcacc ataagcgcca ttgccggaaa gaaagcgttc
     3421 atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat cggtatagtg
     3481 gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa ctcagattat
     3541 tccagtgcgc caaccaaatg atttatcggt cagacttttt ttaaccagat acagagacac
     3601 agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt ttaatggtgg
     3661 tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc cccgtcacat
     3721 gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta tttttcatga
     3781 tttccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg
     3841 gcttatgtct gattttttat agcgcaatcg cactgctcta cattgcgctg tttacctcca
     3901 tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt
     3961 ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg aagaacgtcg
     4021 gcggaaaaat gttgctggtg ctaatggcgg tgattggcat tatcgctaac ccaaacacag
     4081 tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc aatggcgata
     4141 ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg gacacacagc
     4201 ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt attatctctc
     4261 gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg tatctttact
     4321 gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa aacacgctgg
     4381 atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt atatacggat
     4441 tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa aattggccaa
     4501 tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta ctttctatgc
     4561 tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt tcacattgga
     4621 ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt gtttcttttg
     4681 gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca aacaagctta
     4741 agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca ttccacagac
     4801 cta
//
LOCUS       DAZOZD010000279         4796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOZD010000279 DAZOZD010000000
VERSION     DAZOZD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.282"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUPB_003946"
     tRNA            6..81
                     /locus_tag="ACHUPB_003946"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPB_003947"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUPB_003947"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUPB_003948"
     CDS             complement(299..1057)
                     /locus_tag="ACHUPB_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0760623.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUPB_003949"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUPB_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0760624.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUPB_003950"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUPB_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="ACHUPB_003951"
     CDS             complement(3427..4452)
                     /locus_tag="ACHUPB_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0760625.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAZOZD010000280         4796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOZD010000280 DAZOZD010000000
VERSION     DAZOZD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.283"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..354)
                     /gene="rnpB"
                     /locus_tag="ACHUPB_003952"
     ncRNA           complement(<1..354)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUPB_003952"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(387..1532)
                     /gene="garK"
                     /locus_tag="ACHUPB_003953"
     CDS             complement(387..1532)
                     /gene="garK"
                     /locus_tag="ACHUPB_003953"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0760626.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1629..2519)
                     /gene="garR"
                     /locus_tag="ACHUPB_003954"
     CDS             complement(1629..2519)
                     /gene="garR"
                     /locus_tag="ACHUPB_003954"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0760627.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2549..3319)
                     /gene="garL"
                     /locus_tag="ACHUPB_003955"
     CDS             complement(2549..3319)
                     /gene="garL"
                     /locus_tag="ACHUPB_003955"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR0760628.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3335..4669)
                     /gene="garP"
                     /locus_tag="ACHUPB_003956"
     CDS             complement(3335..4669)
                     /gene="garP"
                     /locus_tag="ACHUPB_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0760629.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1151 a         1382 c         1145 g         1118 t
ORIGIN      
        1 ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct
       61 acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag
      121 tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc
      181 ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac tggtcgtggg
      241 tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc
      301 gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg
      361 cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat cgccagtgtc
      421 gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc
      481 aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg atgtacaacg
      541 ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc
      601 acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca
      661 ccggtgatca ccagcgtaca atcgtgaata tgttcttcca gattcagcgc cgtagtgacg
      721 atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc gcccataccg
      781 cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt tttaatgaca
      841 tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt
      901 tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa
      961 gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc
     1021 agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc
     1081 gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctgcc gccaatgccg
     1141 ataataatgt ttgttgcact gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt
     1201 gaagtggtta cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc
     1261 gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc
     1321 tcgcccagcg gccctgtaac ccaggcgtga cgttcagccc cctgggtggc tgcaatcatc
     1381 gcttccaccg ttccttcacc accgtcggca accggaacag aaacatactg tgcatcagga
     1441 aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct
     1501 ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg
     1561 agggcgtttc tcttattcgg cctagattcc aggcccggat tgcaatacac catccgggca
     1621 cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca cgccagggcg
     1681 ctatgatccg ccgttcctaa accatctgca cgcagtgcct gcatcatctc cataaccgca
     1741 gctgtgagca gcagttgtgc gccgacgccg tgagaagtat ccagcgcatt cgccagatcc
     1801 ttaatatgca gatcaatgcg gaagcccggc ttgaagttgc ggtccatcac catcggcgct
     1861 ttggcatcca gcacggtact gcccgccagc ccaccgcgaa ttgcctgata aaccaggtcc
     1921 gggttaacgc ccgctttggt tgccagcgtt aacgcttctg acatcgcagc aatattcagc
     1981 gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc cccggtatgc
     2041 accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg
     2101 ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga
     2161 gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc
     2221 ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca
     2281 ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg
     2341 tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc agcaatcacg
     2401 tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg
     2461 tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata
     2521 tcaaccatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttctgag
     2581 tggcagagcg gaagacgcca agatcgctgc cgacagccac aaacgtcgcg ccccattcca
     2641 gataacgacg cgcatcggct tcgaccggcg cgagaatacc gctgggtttg ccgtgcgcgc
     2701 tggcacggtt aaaaatatgc tgaattgctt tttgtacatc cggatgcgag gcattgccga
     2761 gatggcccaa tgccgcagcc agatcgctgg ggccgacgaa gataccgtct acaccttcgg
     2821 tagcggcaat agcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag
     2881 taatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata ttggtgcggt
     2941 gagaaacgga gacgccgcga atgccttccg gcgggtaacg agttgatgcc accgcctgct
     3001 ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa
     3061 gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg ctggcgctgc
     3121 ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca tgttcgccat
     3181 ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga
     3241 gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg
     3301 ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa
     3361 tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa
     3421 ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga
     3481 caatggaggc aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt
     3541 tcggcgcggt gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag
     3601 ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg
     3661 tggaaaccag caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac
     3721 cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca
     3781 gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca
     3841 gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga
     3901 aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac
     3961 cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag
     4021 agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta tgaatcaact
     4081 tgatccacag cgccgtcagc acaaaaccaa tcccccccat aacggtgaag acgtgctccc
     4141 agccccaagc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc gagaaatatt
     4201 gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga
     4261 caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata aagcgcataa
     4321 agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct tgcaggaagg
     4381 tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa
     4441 gcaaccagcc gccggggatt tgcatcagca agtaggccca gccaaaagca gagaagatgt
     4501 aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca
     4561 gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat
     4621 agctggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt ttatttacct
     4681 cgggtactta tgctgatttt tattattatg gggaaggtgt tatttatgag ttttatttat
     4741 gccgttacga caatgaactc gggaattagt ataagcagcg ggagaataat aatcat
//
LOCUS       DAZOZD010000281         4772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOZD010000281 DAZOZD010000000
VERSION     DAZOZD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.284"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            400..558
                     /locus_tag="ACHUPB_003957"
                     /pseudo
     CDS             400..558
                     /locus_tag="ACHUPB_003957"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            596..883
                     /locus_tag="ACHUPB_003958"
     CDS             596..883
                     /locus_tag="ACHUPB_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0760630.1"
                     /translation="MSPQMIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1325..1858
                     /locus_tag="ACHUPB_003959"
     CDS             1325..1858
                     /locus_tag="ACHUPB_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFR0760631.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2112..2795
                     /locus_tag="ACHUPB_003960"
     CDS             2112..2795
                     /locus_tag="ACHUPB_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HFR0760632.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLETAELIFSDEGFQVVRGYEHQFGVERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2968..3519
                     /locus_tag="ACHUPB_003961"
     CDS             2968..3519
                     /locus_tag="ACHUPB_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760633.1"
                     /translation="MKKQPLLWMTIAAALLSGCTGHREHHPQVTFTVDTVRTDTTASN
                     PICLAEKYGSLLNERHPDLFAGAQGSISRSAFFNDSLTLTPETLHGMIQNTPCVESLV
                     TSVGPDTFTADVSPGVRMQVSPDIHGAPGNITLRLSTTDQKRKQTVEQSISLRPEQSL
                     VVNGFTGDGAGEKRVVLITPHVR"
     gene            3681..3935
                     /locus_tag="ACHUPB_003962"
     CDS             3681..3935
                     /locus_tag="ACHUPB_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFR0760634.1"
                     /translation="MPQQHPGRLQILVVDAHCKRRLFSTKTPTDPDELARRFCTPDNC
                     LVVVLRDNRFLFRLERAPGSHCRWHKGSSSRHQHLQDWLS"
     gene            4102..4398
                     /locus_tag="ACHUPB_003963"
     CDS             4102..4398
                     /locus_tag="ACHUPB_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilJ"
                     /protein_id="HFR0760635.1"
                     /translation="MFTMFFLVKLTIAIVIVCGILSPILVSPHNKLSTLFWKIPNAFW
                     IFLICIYFAIAMAVLIESSEQTRIRRQQYFQQYEVPNETHKNVVIENSIKSQLI"
     gene            4395..>4772
                     /locus_tag="ACHUPB_003964"
     CDS             4395..>4772
                     /locus_tag="ACHUPB_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HFR0760636.1"
                     /translation="MKNNNVPLYLWLLWCTLCLYITTPWQYLRYPYTDNLSLPYMLYF
                     CVVVVICIFPLLFRCVLLIVRSASKYDFTFFVKKKKGSKKSTIHLSPYADTRCPKSMR
                     KHWDVLLSLISKAINNDEIIVMHS"
BASE COUNT         1263 a         1186 c         1044 g         1279 t
ORIGIN      
        1 ctaaaaaacc tttaaaaaat cattttgtag atcattcgat cgtaaataga ataattctta
       61 gataataaaa ccatttgtta cacagcccgc caattgtgta acaacagcct ctacaagagc
      121 atatttgttc atatgttcgt cagaactgcg gattgtctgg acgagggtca gacaccgttt
      181 cgtgcatatg gaccagcatg ttcgaacgcc acaaagcaga agcatcatgc ctggatcgct
      241 tcggcgcgga gcgttaagtt ttgacgcttt tttgtatgtc ttttaagcgg ttagttgact
      301 cggtacgtta tactaagtac cctttactgg tcacaggcaa aactgtgatc gggcgtagga
      361 acccgcgtag tcatcgtcgg cgcagaagtt aatttcttca tttggcgtcg ttttttgcca
      421 taccataaag acaggaactt gtatttcttg tcgtcatcta tggtagctca ggcggggcag
      481 gcattagcct ggccggtttc cgatgacgcc ggtattccta cccccgtctg ggctatcacc
      541 aatgagcgta ggaactcggt gatagcgttt ttagttgtca tcggagaccg ttgctatgtc
      601 acctcaaatg atacagaaac tcccggccat cactctgttg gaaggcatgt ttcccgagct
      661 atcaacaaac cagcttaaag tctgcgtttt ttacgcaatg ggtgttcctt acgacgccat
      721 tgctcagaac tgtcggcttt ctcccgaaac ggtacgaacc tacctgaagc gaagcctgaa
      781 gaacttaaat ctggaaggat atgatgcact tcgctctgct gtgctgatgc gaacgttcgt
      841 tttcatgatt agcaatacag ctaaagaaaa cgaaaaaatg tgaacaggtg tccccattaa
      901 aggggacact ttcatggaaa ttgcattttg cactttacag gcgatcccaa attgataaac
      961 ataaacacca agcacaagtt actttaataa agcaaatcac ttcagacggt gtcccagtta
     1021 tttgtatctg gctcatgccc gatatatggc gaccttactc tgaactgacg tgctgatgtt
     1081 ttatttcgcg tatcacaata ctgtgccact gtattgctga tattagtgct tagccgttct
     1141 ttatctgtga gttcgccaaa tcaccgatac agaacgtact agagtctgct ggttatatgc
     1201 ctgtcagaac tgcagtttat ccagatatgg gctggacact gcttcagaca tatgatttag
     1261 catattcgcc tgacatcgca aaatcgtcca tcatggatcg gttttgcgcg gagcgttgaa
     1321 cttcgtgaat atagaacacc tgaataaccg gaactggtat cttgcgcaat ataatacagc
     1381 cggcaaaaac agagaatctt tattctcatg gctgaatgag caaaacgtcg taccatggac
     1441 tccattaata acaagaaaga taagacgggc agactccagg tgctgttacc gtgaaagaat
     1501 attcgctatt tttccagggt acttttttat tctggctaat tttgacattc acccagtatc
     1561 agcattacgt cgacatagcg ccttcattga tttcgtgaag tttggcggtg aaataaaacc
     1621 cgtcaataaa gatattgttg atggattaat gaagatttac ccggaccctg tgctaaatcc
     1681 gggagccagg gaagagctga atgcggcttc cagcatatgg ctgacaaaag cacaatatca
     1741 gtatctcctc agaatggaaa atactctcca gcctgagtcg aggatatccc tgctcctgga
     1801 actggtatca aatgccgaac atcacggctt tatggagcgc cttgtaaaca tcccatagcc
     1861 agtatttccc ggccttcggg ccgggctttt ttcaattcca gagaatattc cggagtgaag
     1921 cctgcacgaa gccatcccca ccatggactc cccctgtctt ttgatggaac cgtgtatttc
     1981 ttaatggcga aagaaataaa aggcttcacc ccagagtatt gattcgtttt atcaaaggcc
     2041 ggcccgtgcc atcacgtgtg gcggagagaa cggataccgg tcttcttttc atctcacagt
     2101 aggacattcg catgaccacg acagaacatc ccgtgcgcca gttgctctgg tgtgcactga
     2161 atgccctgaa aacggcacag gaaaaccaca atataaccag tgaaaccgga ttacggcatt
     2221 acctgcttga atggctttct ggtgccagca gacatcctga gttccgtagt ctccctggcg
     2281 aaatcatggc gttaaaagca ttgaccgaaa aagatcgcaa tattcccatc atcggaacgc
     2341 tgaatacgct ctttctcagc tctgccactg tcggggaatg tccgttgttt cgttttcggg
     2401 ctgcactggg taaattacag aaagcaggct ggcgcacata tgtctgccca tggcctgaga
     2461 gggtgttcaa tgaatccatt gaacgggcct gttccgggaa acgtcatctg ctacaactaa
     2521 gtcgcaccga agagtgtttt ctaccgacag gcgatatgag ggcaccagtt acgttgcagc
     2581 tgatcactcc actacaccgc aaaactatgc agcttctgga aacggcagag ttaatattct
     2641 ccgacgaagg atttcaggtg gtgagaggat acgaacatca attcggggtc gaacgcagag
     2701 aaaccctctt tcacacccta tttatcgggc tgcccagcct gccagaagat gtctggggtg
     2761 caacacagga gcagacgact atcacattac actgaggatg tgtaatcccc ggcatacccc
     2821 gtttccagtc ttcgcagcct gtaccgccct tcctgccgtt tccgacattc tttctgtcac
     2881 tactcgcttg tgccattttt tcgcaagcgg cccccatcga catgacggtg cgagtctttc
     2941 tttttaccct gagaggaaat taacagcatg aaaaaacaac cactactctg gatgacgata
     3001 gccgccgcac tgctgtccgg ctgcacaggc cacagggaac atcacccgca ggtgacattc
     3061 acggtcgata ccgtgcgaac agacacaacg gccagcaacc caatctgcct ggcggaaaaa
     3121 tacggaagcc ttcttaatga gcgccatccg gacctgtttg ccggtgctca ggggagtatt
     3181 tcacgcagtg cattttttaa tgacagcctg acgctgacac ctgaaacgct ccacgggatg
     3241 atacagaata cgccctgtgt tgaatcactg gtaacgtccg taggtccgga taccttcact
     3301 gcagatgtct ctcccggtgt cagaatgcag gtatcaccgg atattcacgg tgcgcccgga
     3361 aatatcactc tgcgactctc gaccacagac cagaaacgaa aacagactgt tgaacaaagc
     3421 atttctctca gaccggaaca gtcccttgtc gtgaatggat tcaccggaga cggagctggc
     3481 gaaaagcgcg tcgttctcat cacacctcac gtgcgttaat tcccaacaca acccgtttcc
     3541 cgtcttcgca gcctgtaccg cccttcctgc cgtttccgac attctttctg tcactactcg
     3601 cttgtgccgt tttttcgcaa ccggtacgtg cagttatgac ggtacagctg cttcttttcc
     3661 cgttccggaa ggagtctttg atgccacagc aacatcccgg acgactgcaa atactggtcg
     3721 tcgacgcaca ctgcaaacgc aggctgtttt caacgaaaac accgacagac cccgatgagc
     3781 ttgcccgacg tttctgcacg ccggataact gtctcgtggt ggtactacgc gacaaccgat
     3841 ttctgttccg actggagcgt gctcccggca gccactgccg ctggcacaag ggcagcagct
     3901 cccgccatca gcatctacag gactggctgt cgtaaacaca acaaaaacaa aagacctcgt
     3961 caaaacaggt gggggccgtg ttctttttag ttaaattaac catcgcgatt gcgattgtct
     4021 gtgtacacct tttaacgatt ttaatttagc cgtttgttgc catctttatt acctctctcc
     4081 cttttatcat ggagattaac gatgtttacc atgttctttc tagttaaatt aaccatcgcg
     4141 attgtgattg tctgtggaat cctttctccg attttagttt caccacataa caaactgtcc
     4201 acactttttt ggaagatacc aaacgcgttc tggattttcc tgatttgcat atactttgca
     4261 atcgccatgg cggtgttaat agaaagcagt gaacagacac gcatccgccg ccaacaatat
     4321 ttccaacagt acgaagtgcc aaatgagaca cataaaaatg tggtgataga aaacagtatt
     4381 aaaagtcaac taatatgaaa aataataacg taccattata tctatggttg ctttggtgta
     4441 cgctttgtct gtacatcact accccatggc aatacttacg ttatccatac actgacaact
     4501 tatcattgcc ttatatgtta tatttctgtg tggtggttgt gatttgcatt ttcccactac
     4561 ttttcagatg tgtacttctt attgtaaggt ctgcatctaa atacgacttc acattctttg
     4621 tgaagaagaa aaagggaagc aagaaatcaa ctatacatct tagcccctat gctgacacac
     4681 gttgcccgaa gtcaatgagg aaacactggg atgtattgct ttctctgata tcaaaagcaa
     4741 ttaataatga tgaaatcatt gtcatgcatt ca
//
LOCUS       DAZOZD010000282         4766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOZD010000282 DAZOZD010000000
VERSION     DAZOZD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.285"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUPB_003965"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUPB_003965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0760637.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUPB_003966"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUPB_003966"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0760638.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1042 a         1342 c         1230 g         1152 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttaatta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atattttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgct
//
LOCUS       DAZOZD010000283         4766 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOZD010000283 DAZOZD010000000
VERSION     DAZOZD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.286"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(95..856)
                     /gene="kduD"
                     /locus_tag="ACHUPB_003967"
     CDS             complement(95..856)
                     /gene="kduD"
                     /locus_tag="ACHUPB_003967"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0760639.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(921..1757)
                     /gene="kduI"
                     /locus_tag="ACHUPB_003968"
     CDS             complement(921..1757)
                     /gene="kduI"
                     /locus_tag="ACHUPB_003968"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0760640.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2044..3225)
                     /locus_tag="ACHUPB_003969"
     CDS             complement(2044..3225)
                     /locus_tag="ACHUPB_003969"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0760641.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3480..4709
                     /locus_tag="ACHUPB_003970"
     CDS             3480..4709
                     /locus_tag="ACHUPB_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0760642.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1240 a         1206 c         1055 g         1265 t
ORIGIN      
        1 atggattatt tcataaccat gatatgaatt atgatgacct acaggtataa aaaaccctgc
       61 catgaggcag ggtcataaaa gtaagaggaa tgaattaacg agccagccaa ccgccatcta
      121 cggcaatggt ataaccattc acataatctg aagcgctgga ggcaaggaac actatcggcc
      181 ccatcaggtc actcggcagt ccccaacgac cagctggaat gcggtcgaga atttccgcgc
      241 tacgttgttc atctgcccgt agttgttgag tattgttggt cgccatgtaa cccggagcta
      301 tcgcattaac attaatgttg tgtttagccc attcgttcgc catcaatcgc gtcacgccca
      361 tcacgccgct ttttgatgcg gtataagaag gcacacggat cccgccctgg aaggagagca
      421 ttgacgtgat attgataatc ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg
      481 cctgagacat gaagaatacg ctcttgatat tcaggttcat gacatcgtcc cagtcttttt
      541 cgctgaactc gagagcatct tcgcggcgaa tcaatccggc gttattcacc aggatatcaa
      601 tatgaccaaa ctcagctacc gcgcgatcca gcagtgctgg aatgccatca atctttcgca
      661 gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt gacctgcttg atggtttcag
      721 tcggttcaac gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca
      781 tcccctggcc cagtccagta tcacaaccag tgacgaccgc aactttacct tcgagagaaa
      841 atgcacttaa aatcataaca ataccttatt cgtttatgcc gacaactagc gcaaatcttt
      901 aacggccact atgccgacaa ctagcgcaaa tctttaacgg ccacatggtc catatcatca
      961 aagacctggt tttcaccaac catgccccag ataaaggtat aagctttggt tccgacgccg
     1021 gaatggatcg accagctggg ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc
     1081 gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat attgaaatag
     1141 aaataaactt ccatccggcg ctcgtgagtg tgacacggca tggtgttcca caagttacct
     1201 ggagccagct ccgtcagccc catactcaat tggcaggttt ccagcacatc cgggacaaaa
     1261 tatttgttaa ttgtgcgacg gttactggtg aggttatcgc ctaacgtgac tggagatact
     1321 tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa
     1381 aactttgccg gagtggcggt atcaatactg gcaaagacaa cttcttttgc acctttacca
     1441 acatacaggg cgtcgcggtg accgatttca tagcattggc catcgacagt aatcgtaccg
     1501 gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaatagct tacgccgagt
     1561 tgtttaccaa cttccccgcc aacggaaacc gttttagtta tcggcataat gccgccaaca
     1621 ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa taccttttca
     1681 accaaaaatt cattgcgcag cccttgggta tccagcgttt ttgcgtgcgc actgtggatg
     1741 ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat
     1801 agtgaaacac tgtttcgttt tctctataaa gggatgatat gcccatgggc tagggttttc
     1861 aattaaaaat aaaacaatgt ttcattttta ggttaaggat taaaaaaagt gcagtgtcga
     1921 tcacgaatat gtcatgagaa tggaaaaatg agggagccca gaaaatgtat gtcgacttgc
     1981 cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg aggaaatgca
     2041 gagctactcg tcacgttcaa tggtcaatgc cacgccctgg cccccgccga tacaaagcgt
     2101 tgccagccct ttgcgggcat tacgtttcac catttcatga accagagaaa ccaggattcg
     2161 gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga cattgacccg
     2221 acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt
     2281 agcctcgata agatcgacat cagccaactg ccagcctaca cgctccaggc aacggcgggt
     2341 cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg
     2401 gatgcgggcc agcacgggta aattcaacgc tcgtgctttg gcttcgctca tcatcattac
     2461 cgcagctgcg ccatcgttta tggatgatgc attccccgcg gtcacagaac cgagactatc
     2521 aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc
     2581 ggtatcaaca accaacgtct gaccgttact ttggatcatt accgggacga tctcatcttt
     2641 aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc
     2701 ctgcaactga cggctgatgc catattcgcg agccagattt tcggcggtga caccaatatg
     2761 ataatcattg aaggcatccc acaacccatc atgcaccaga ctgtcaacca actggctatt
     2821 gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc
     2881 ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag tagccagatg
     2941 cagtgcttta agtccggaac cgcaaacgtc attaatcgtg attgcagaaa cgctattagg
     3001 aagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg cagtcaacac
     3061 ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc
     3121 tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac cacgaaagca
     3181 gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa cctcgcacca
     3241 attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt taaaaaaagt
     3301 gatttttatc acaaaggaaa tatgcctgag cagtagtcag agtcataact ggcacgtaag
     3361 gtttgcaacc actaacccac caatagatgg gtagataagg cgttaatctc ccatacttaa
     3421 cctggtttat ggtaaattgc cctccatttt gtttaatttg tagatgatac gttcagataa
     3481 tgtctaatat ttggtctaaa gaagaaactc tgtggagttt cgcgctctac ggcacagccg
     3541 ttggtgcagg cacgctcttc cttcctattc agttaggttc ggcaggggct gtggtcctgt
     3601 ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc ttatgccagt
     3661 tcatcctctc atcaaaaaca tcagcatgtg aagggataac gggcgcggta acacactact
     3721 atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt tttgtcgtcg
     3781 tgttgatata tgcagtggca attaccaact cacttacgga acagctggca aagcatatgg
     3841 ttattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatt ctgaatctca
     3901 tttttctgat gggacgtcat gccactattc gggtaatggg atttttggta ttcccattga
     3961 ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct gatctattaa
     4021 cgacccaggt agagttcaat cagaataccc ttcaccagat atggatatcg attcccgtga
     4081 tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac agacgtgaaa
     4141 aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct tatctcatca
     4201 tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca ccttcgtata
     4261 ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg ctgccgaacg
     4321 ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat
     4381 tcctgggtac gtactttggc gttattgaag gtgccacgga ggtcgtcaaa acaacactac
     4441 agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc actatcaatt atgttggtat
     4501 cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg atttatgcga
     4561 tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgttgtca acgtatctga
     4621 tcccggcgct taaaccctgg cgctccatcg gaaatctgat tacgctgatc gtgggtatcc
     4681 tgtgcgtatc ggtaatgttc tttagctaat atccgttgtg gatggttcaa aattgctaca
     4741 attttgaacc attccatttt tcataa
//
LOCUS       DAZOZD010000284         4760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOZD010000284 DAZOZD010000000
VERSION     DAZOZD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.287"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHUPB_003971"
     CDS             <1..279
                     /locus_tag="ACHUPB_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0760643.1"
                     /translation="YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
                     NRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            620..2272
                     /gene="ushA"
                     /locus_tag="ACHUPB_003972"
     CDS             620..2272
                     /gene="ushA"
                     /locus_tag="ACHUPB_003972"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFR0760644.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2283..2453)
                     /locus_tag="ACHUPB_003973"
                     /pseudo
     CDS             complement(2283..2453)
                     /locus_tag="ACHUPB_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2586..2705)
                     /locus_tag="ACHUPB_003974"
                     /pseudo
     CDS             complement(2586..2705)
                     /locus_tag="ACHUPB_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2779..3327)
                     /locus_tag="ACHUPB_003975"
                     /pseudo
     CDS             complement(2779..3327)
                     /locus_tag="ACHUPB_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="ACHUPB_003976"
                     /pseudo
     CDS             complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="ACHUPB_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1273 a         1151 c         1111 g         1225 t
ORIGIN      
        1 tacacacagc ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg
       61 tatgacaacg cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
      121 aagagctgga aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat
      181 aataatcgac gattgctgga aaggctgggc cacatccctc cggcagaagc agaaaaagct
      241 tattatgctt ctatcggaaa cgatgatctg gcagcctgag ttcacagata aaacattctc
      301 caggaaactt ggggcggttc acgtcagtat cagaccatat taattgctga aactgataaa
      361 catcaaacct tatagaaggg tgaatgctga aagggggcta tggtgatcga cgtgatgaca
      421 acgacaggta agaccgtgac ctgataaacc tgaattgtgt cctagacata aagtccgtga
      481 agggaataag gctcaggtca gcgaatctta tagtggcccg cagctctgac tgcaataagg
      541 ccggatacag agcagcaact actcgtcgat gtcagaacaa cggaagcctt gcaaacgggg
      601 atgcgttcat acaggtacaa tgataccgtt aaaaaaaaac attacgctaa taatgttcac
      661 cctgtcatta ctgacaggaa atcctgctat agcgtatgaa acagataaag tatataaaat
      721 aaccgttctg catacaaatg accaccatgg acacttttgg cgtaacaatc atggtgagta
      781 cgggttatcc tcccaaaaaa ctctggtgga caatatccgg cagaaagtaa taaataatgg
      841 cggaagtgtt ctattattat ctggtggtga tattaacacc ggtgtacctg aatcagacct
      901 tcagaaggct gaacccgata ttcgtggaat gaatctaatt ggttatgatg caatggccgt
      961 cggtaatcat gaatttgata atcctctaaa tatattacgt cagcaggaaa aatgggccac
     1021 atttcctttc ctttctgcta acatatatca aaaaagtaca gggaggcgtt tattcagtcc
     1081 atggaaaatt ttcatccgtc agaatttgaa aattgcagtc attggcttga cgacagatga
     1141 tactgcgaaa acaggaaatt ctgaatattt tactgatatt gagttccgtc aacctgctgc
     1201 tgaggcgagg agtgtcattg atgaacttaa tcagcaggaa aagccagata ttattattgc
     1261 ggcaacacat atgggtcact atgacaatgg cgagtcaggt tcgaatgctc ctggtgatgt
     1321 tgaaatggct cgcagtttgc cgactggctc attagcaatg attgtgggag gacattcaca
     1381 agctcctgtc tgcatggcca gcgataataa aaaacagtgg aattatattc cggggacaac
     1441 ctgtgtacca gataaacaaa atggcatctg gattgtacaa gcgcatgagt ggggaaaata
     1501 tgtcggtcag gctgattttg aattctgtaa cggtacaatg aaactggtta attatcagtt
     1561 acacccagtt aatctcaaaa tgagaattac cagagaagat ggaaaaacgg aattttcttt
     1621 ttacaccccc gaaattactg aagatccaca gatgttatcc ttactgacac cattccagaa
     1681 taaaggcaaa gcacaactgg atgtgaaggt tggggttgtg aacggacgtc tggaagggga
     1741 cagaagtaaa gttcgttttg ttcagaccag tatgggccat ctgattctga gcgctctgac
     1801 agaacgtatt gacgcagatt ttgctgttgt cagtggtggg gaaattcgag attccataga
     1861 gagtggtaac atcacataca aagatatact gaaagttcag ccatttggta atacggtagt
     1921 aagtattgat ttgacgggga aagaagtagc tgactaccta gctacagtgg cacagatgaa
     1981 accggattcc ggagcctacc cacaattttt gaataccagc tttgtagtca aaaaaggaaa
     2041 aatcgaaatg ctaaaaataa aaggtaaatc ggttgacctt aacaaaaaat atcgtatgac
     2101 aacatttagc tttaatgcta ctggtgggga tggctatcca cgtattgata atagacccgg
     2161 atacataaat accggattta ttgatgctga agtacttatc gaatatatcc gggaacattc
     2221 accgctggat gctgcttcat atgaaccaaa aggtgaagtc agttggcagt gataacgtaa
     2281 ttgacgatca tcacgtgttt tttgctgaca gtcgctgtga ctttgtgaaa gtagtctctg
     2341 cggatgtttg cgatatggga atgcagttgc tgtattttgt gtttctgctt ctgccagttg
     2401 ttgctgaatt taaccttgcg gcttaactga tgttggagcg ctgccagctt cttcgtcaat
     2461 ttcgctgatt aaagtttatt cattatggca tgatctgcgt aagcggctcg tcagaaccgt
     2521 attgatattt actaagagct cagatcaact ttccagggca acagatcgcg tacccggttt
     2581 gccggtcagt cctggatatg ctcaatgacg taacgcagcc atttttctgg ctccacattg
     2641 ttcagacggc atgtgccgat cagcgagtac aacaccgccg catgttcgcc accgctatcg
     2701 gaaccccgcc ggagagagtt tcactctccg gctgctcacg gaaccgtacg tgacactctc
     2761 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gggcaaatag
     2821 cttcggatac tgtttcctta ccagcgccag ctttcgctga gccttccggt tcgaaagtct
     2881 gtatttagac tgcatccact taagcaaacg tgactgcatt gcactccaca gtcgatagct
     2941 gaagtttctg gaccagaact ttccatagta ctcaatccag cctctcacta tcgcattgta
     3001 gcgcctcgca aaatccagca aactctcagc tgttgagcga tgtatacgcc acttcttgat
     3061 tgtttctgtt attttgcaca ttgctgcatt tgatgcaccc ggcatacatt ttctgtacaa
     3121 ttcgcctttg aaattcttca gagtacgcac tttgaagtca tatccgagaa aggagaaact
     3181 cgtagcgacg tttctccttt taaacgtgtc aatgtaggcg atattcgtct tccctgcgtt
     3241 caggaccaac ccgacttccg agaagcgcac cgataacctg ttcttcagcc ttgttgcatc
     3301 actcattcgt gaacaatgta ctacaagtcg agtcacccga gggaacctcc ccctctggtg
     3361 ctcacggaac cggacgtgaa cctctcagct catccggctc ttctcattga ggcctgtccc
     3421 catagtaacg gccagtgtgc aaacaacctc gtttcactcc gcgcaacacg ggccagccag
     3481 tggctggccc gtcgtcggtg gcccctaagt cgcttgtgtt tacggggcag ccataacacg
     3541 agtttgcgat cgatttgcct cagcgtcaga tacagtgctg atttataaaa agcaccgtag
     3601 tactttatcc agcctctgat tttcgcattg aacatatgcg ccagctcgta caaggacttg
     3661 tcactgcgat tctcctcacc tcctgacgga ttgcttttcc tgctctggca ctcatcgccg
     3721 gactgaagtt aacgaaggtc ttaccccagc gattcatcga cagacgcggc cggaatgtat
     3781 agcccagaaa gtcgaagctc gtttccgggt agtccccccg tcggtcagca tccttacagt
     3841 aaactattcg tgttttttgc ggatgcagct gcagcccgca ttgcgcaagc cgctgcctca
     3901 gtccacttat cagagcgtca gcctgccact ggctgtgaca gtggcataca acatcatcgg
     3961 cgtacctttc gaagggaacg ccgggaaact gacgttgcat ccacatatca aaggcatagt
     4021 gcaggaacag atttgagagc aggggggaga ttacgccgcc ctgcggcgtt cctcgacccc
     4081 gtgccagcat tgctccatcc ggcatttgca ccggtgcctt cagcctgcgc tcaatgtaca
     4141 tcaccaccca gcgtgcctga gtatgatgct gcactgtctt cagtagcagg tcatgatcga
     4201 tatcatcaaa gaagccttta atatcgactt ccaccaccca gtcaaacttc cagcagcgtt
     4261 tcctcgcctg cgcaatcgcc tggtgtgcgg atttacccgg tcgataacca tacgagtctg
     4321 catggaacag gggttccagt accggctcga gtatctgctt aacgaccatc tgggcgatcc
     4381 ggtccgacac ggccggaata ccgagcggac gtacaccacc gtcagctttg ggaatgtcca
     4441 cacgccttac cgcctgcggc atataactgc cggaagccat tctgttccac agtttgtaga
     4501 gattgtctgt aacattctcg tcaaacccgg ccagtgtctg cccgtcaatg cctgccgccc
     4561 cacggttggc ttttactcgt ttataagctt cccatacctg tcgtttactt acaggaaatg
     4621 gctttgccga ttttacagca gtcctcccat cggcattgtc gcccgtggtt gccgccgcac
     4681 catcagcaca atggggcaag ccctttgctc cggacccatt acggtccttc accgctacta
     4741 cggtttactc cgtccctgta
//
LOCUS       DAZOZD010000285         4746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOZD010000285 DAZOZD010000000
VERSION     DAZOZD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.288"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHUPB_003977"
     CDS             complement(<1..737)
                     /locus_tag="ACHUPB_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0760645.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUPB_003978"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUPB_003978"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0760646.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUPB_003979"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUPB_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0760647.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHUPB_003980"
     CDS             4397..4678
                     /locus_tag="ACHUPB_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0760648.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1148 a         1178 c         1185 g         1235 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 agatgc
//
LOCUS       DAZOZD010000286         4743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZOZD010000286 DAZOZD010000000
VERSION     DAZOZD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.289"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUPB_003981"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUPB_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0760649.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUPB_003982"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUPB_003982"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0760650.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUPB_003983"
     CDS             2475..4124
                     /locus_tag="ACHUPB_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0760651.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..>4743)
                     /locus_tag="ACHUPB_003984"
     CDS             complement(4278..>4743)
                     /locus_tag="ACHUPB_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0760652.1"
                     /translation="SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLE
                     MVICTDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILR
                     GEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT          979 a         1133 c         1321 g         1310 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gag
//
LOCUS       DAZOZD010000287         4727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOZD010000287 DAZOZD010000000
VERSION     DAZOZD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.290"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            247..1116
                     /locus_tag="ACHUPB_003985"
     CDS             247..1116
                     /locus_tag="ACHUPB_003985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0760653.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUPB_003986"
     CDS             complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="ACHUPB_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR0760654.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1881..2117)
                     /gene="rclB"
                     /locus_tag="ACHUPB_003987"
     CDS             complement(1881..2117)
                     /gene="rclB"
                     /locus_tag="ACHUPB_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR0760655.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(2226..3551)
                     /gene="rclA"
                     /locus_tag="ACHUPB_003988"
     CDS             complement(2226..3551)
                     /gene="rclA"
                     /locus_tag="ACHUPB_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR0760656.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            3777..4631
                     /gene="rclR"
                     /locus_tag="ACHUPB_003989"
     CDS             3777..4631
                     /gene="rclR"
                     /locus_tag="ACHUPB_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR0760657.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1305 a         1028 c         1047 g         1347 t
ORIGIN      
        1 cattctccat taattcttcg tgatatcgca cattttcgtt atgcccaagg ccctaagtgt
       61 atcaaaaaca gcaaggtcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
      121 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
      181 cacatactaa tttgaggtta tgttttaacg tagactcatt gttcatgcct aatggaggga
      241 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
      301 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
      361 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
      421 gttcgtgaag ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa
      481 ttgtgggggc aggatatgtt gaatcaggat acggcagcag caggtattga agcatcatta
      541 aaaaaatcgg gactagagta cttcgacctc tatttattgc accaggctat gcgcgattat
      601 ttcagtgtgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg
      661 gtttccaatt tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
      721 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
      781 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
      841 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc
      901 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
      961 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
     1021 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt
     1081 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcatatat tttacctgag
     1141 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
     1201 atatggcgtc aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag
     1261 acagtgttcg gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg
     1321 tttaagaatt tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt
     1381 atctttcaat accaagcgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc
     1441 caatgcgggt acccatgcct ccggggtggt gattaaaaat gagagtgtta ccagcggcgt
     1501 ggtatatgcc atcagtcctc ccaataaacc taaccagcga ttgacaggat tagccaaaac
     1561 cagcagagca ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt
     1621 ggccgattgc catgcccttg cttctggttt gtattcgcct tcgtgagtca gatactgttt
     1681 atagtcttcc gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt
     1741 aatgctgtct gcctcgtaag ggacaaactt taataaccca atccacataa aaacaatcgc
     1801 aatactcaga cgaatcaatg acaggccaat tttatctccc cgacttaaca ggtgcaggta
     1861 tttttccatt gtgaacatcc ttatttatac aatactgctg ttgcggttga attattatca
     1921 attcgcatcg atgtgacttt ccaggatgaa gcattatatt ttctggctat atcagcaatg
     1981 gtatcctcca gaactgtcgg tgatgctgat actgacgaga cgctgatatg tttcagaatt
     2041 attttatcat ctgcattggt ggaaaattcc ataacgccag ataatagtgt tgcaaataaa
     2101 acagattttt taaacatata aaataccatc aatgaagtga ttaagaagtt atagagtaac
     2161 agagaattaa taagattctt ttctctgaga cgccagaata tttgttctgg cgtctgattt
     2221 tgagtttatt tgactaatga aaatagatca ttgagtgatt cgctcatcga cggatgagta
     2281 aatatctgat cgcgtaatat gctataaggc agcccggcat ccatcaccat tttcactata
     2341 ttgatcatct cgtgggagtc aacacacagc agtgatgccc ctaatatacg ttgggtttta
     2401 ttatcaacaa tcgcttttaa taccccacga gtatcattca tcactctggc acgcggaatt
     2461 gcagctacag gcaatgtcac cacctgaata tcagcaccac tctctctggc ctgttcttct
     2521 gtcataccaa ccctggacag tggcggtgtc atgaatacgg aataaggcac atttttccga
     2581 tcatcagtac tacgtctgcc ttctcccagc aactcatcac gcacaatgcg gtaatcatcc
     2641 agtgaaatat aagtaaattg cagcccgccg gtaacatctc ccatcgccca aatattgtct
     2701 gcggtggtat gtaattgctt gtcaacgaca attgccccgc gctcgtttac tgcgataccg
     2761 gcattttctg gatgtaacga agcggtagcc ggttgacgac cggaagctat taacagtgca
     2821 tccaccgcca gttgggcgtg ctcgctatgc acttgcactt gattttcatg gtgactgatt
     2881 cgctccacat gggcattgag gataatatcg acgccctgat cgcgtaaaat cgtcgcgata
     2941 ttatcagcaa tatcccgatc ttcccgaggc aaaaacagcg aagctgcttc taaaatggtt
     3001 accttgctgc caaaattagc gaacatagag gcgaactcaa cgccaatata tccgccgccc
     3061 aaaataccta aatgcccagg caattctttt agattaagta atccggtgct gtcatataca
     3121 ccaggcgtgg tggtaattcc aggaattggc ggaaccacgg tttgtgcacc ggtattaata
     3181 aaaattttct cgccatgaat ttccagattt ccctcaggcc gatgaacacg caggctatga
     3241 ttattgataa actccgcctg gccgtcgatc acgtcgatat tgggcatatc cgcaagatta
     3301 tgaaaattct tattacgtaa aaaattaacc acttcatttt tacgctgtat ggcacggaca
     3361 aaatctgtgt gctgctgtgc gtcatgaacc aatgttttgg ttgggatgca gccgatatta
     3421 atacaggtcc cgccatacat tgcatttgat tgttcgatga gagccactcg ccaacctgct
     3481 tttgccagcg tgacggctaa tgtttttcca gccttgccaa aaccaataat aactgcctga
     3541 tatttattca tgatgatttc ctgtcgaaac acgttcattt gacatcatga ctatagacgc
     3601 aaaagagcaa ataccatttc ttcacaacgc ttgatatttt gtcttatcgt ctggtatttt
     3661 ctttttctta atcagaatca caaaacaaaa atcatgatta accatttgat ggtattagat
     3721 tagttattta ctaagattgt tggtgtttgt aatcaaaaac cactcaggag tctgatatgg
     3781 atgcccttag ccgtttgttg atgcttaacg ctccacaagg aacgatcgat aagaattgcg
     3841 tgttaggaag tgactggcag cttcaacatg gtgccgggga attatcggtt attcgttggc
     3901 atgcgttaac gcaaggagcg gcgaagctgg aaatgccgac gggagagatt tttacattac
     3961 gcccgggaaa tgtggtcctg ctaccacaaa attcagctca tcgcctgagt catgtcgata
     4021 atgaatcgac ctgtattgtc tgcggcactc ttcggttgca acattcggcg cgttattttt
     4081 taacgtcttt gccggaaacg ctgtttttag caccggttaa ccacagcgtg gaatataact
     4141 ggctgaggga ggcgatcccg tttttacaac aggaatccag atcggcgatg ccgggagtgg
     4201 atgcactgtg tagccagatc tgcgctacat tctttaccct cgcggtgcgt gagtggattg
     4261 cacaggttaa tacagagaaa aacattctca gtttgcttct gcatccacgt cttggtgcgg
     4321 taatacagca aatgctggaa atgccaggac acgcctggac cgtcgaatcg ctggccagca
     4381 tcgcccacat gtctcgggca agttttgccc agcttttccg tgatgtttcc ggaaccacgc
     4441 cgctggctgt attaacaaag ttgcgtctac aaatagcggc ccagatgttt tcccgggaaa
     4501 tgctccctgt tgtggtgatc gctgagtcag taggctatgc cagtgaatca tcttttcaca
     4561 aggcgtttgt ccgcgagttt ggttgtactc cgggagaata tcgggaaagg gtcagacagc
     4621 ttgcaccctg aataaaaccg ccagaaatca gggcaaagat aatccgcatt ccgggagttg
     4681 tgagcctttc aactatttct atttccaacg gtggtttggg ctttata
//
LOCUS       DAZOZD010000288         4713 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOZD010000288 DAZOZD010000000
VERSION     DAZOZD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.291"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            97..1071
                     /gene="hemB"
                     /locus_tag="ACHUPB_003990"
     CDS             97..1071
                     /gene="hemB"
                     /locus_tag="ACHUPB_003990"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0760658.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1177..2028)
                     /gene="tauD"
                     /locus_tag="ACHUPB_003991"
     CDS             complement(1177..2028)
                     /gene="tauD"
                     /locus_tag="ACHUPB_003991"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0760659.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2025..2852)
                     /gene="tauC"
                     /locus_tag="ACHUPB_003992"
     CDS             complement(2025..2852)
                     /gene="tauC"
                     /locus_tag="ACHUPB_003992"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0760660.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2849..3616)
                     /gene="tauB"
                     /locus_tag="ACHUPB_003993"
     CDS             complement(2849..3616)
                     /gene="tauB"
                     /locus_tag="ACHUPB_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0760661.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3629..4591)
                     /gene="tauA"
                     /locus_tag="ACHUPB_003994"
     CDS             complement(3629..4591)
                     /gene="tauA"
                     /locus_tag="ACHUPB_003994"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0760662.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1074 a         1378 c         1220 g         1041 t
ORIGIN      
        1 ttctcttctt gtcaacatca cgacaacttt cgtaaaacac tcctgccccg cttcaggtat
       61 actgtgcacc ttaattccac aaacatcagg cagaccatga cagacttaat ccaacgccct
      121 cgtcgcctac gcaaatctcc tgcgctgcgc gctatgtttg aagagacaac acttagcctt
      181 aacgacctgg tgttgccgat ctttgttgaa gaagaaattg acgactacaa agccgttgaa
      241 gccatgccag gcgtgatgcg cattccagag aaacatctgg cacgcgaaat tgaacgcatc
      301 gccaacgccg gtatccgttc cgtaatgact tttggcatct ctcaccatac cgatgaaacc
      361 ggcagcgacg cctggcggga agatggactg gtggcgcgta tgtcgcgcat ctgcaagcag
      421 actgtgccag aaatgatcgt catgtcagac acttgtttct gtgaatacac ttctcacggt
      481 cactgcggtg tgctgtgcga gcatggcgtc gacaacgacg cgactctgga aaatttaggc
      541 aagcaagccg tggttgcagc tgctgcaggc gcagacttca tcgccccttc cgccgcgatg
      601 gacggccagg tacaggcgat tcgtcaggcg ctggacgctg caggctttaa agatacggcg
      661 attatgtcgt attcgaccaa gttcgcctct tccttttatg gcccgttccg tgaagctgcc
      721 ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga acccaatgaa ccgtcgcgag
      781 gcgattcgtg aatcactgct ggatgaagcc cagggcgcag actgcctgat ggttaaacct
      841 gctggagcgt acctcgacat cgtgcgtgag ctgcgtgaac gtactgaatt gccgattggc
      901 gcgtatcagg tgagcggtga gtacgcgatg attaagttcg ccgcgctggc gggtgctata
      961 gatgaagaga aagtcgtgct cgaaagctta ggttcaatta agcgtgcggg tgcggatctg
     1021 attttcagct actttgcgct ggatttggct gagaagaaga ttctgcgtta atttttttcc
     1081 tctcagaaag ggggagttaa cctgttgaaa gtacggtgaa cgccccctca ccctaaccct
     1141 ctccccaagg gggcgaggga accgtccgca ctcgtatcac cccgcccgat aaaacggttt
     1201 atccccaagg atcgtcgccc gatgcataat ccgccgctgt ggcaggtaat cggcattggc
     1261 atagtgctga gtcacgcggt tatcccaaat cgcaatatca ttcggttgcc agcgccaacg
     1321 cacctgaaac tccggtttag tgatatgggc gaataaaaag cctaacaagg cttcgctctc
     1381 tttctcgctg acatcgacaa ttcgcgtagt aaagccttca ttcacaaaca gcgcctgttt
     1441 accgctcacc ggatgcgttc gcaccaccgg atgtagcaac ggaggatttt tcgcgaccgc
     1501 ctcgcgccag cgttgatgtt cctcctcggt tttgcggtat ttgtattccg ggaacgattt
     1561 acggaaatca tgctccgcac gcagcccgct cagcagctgg cggaagggaa cagagagcgc
     1621 ctcataggcc gcaataccgc tggtccagag cgtatcaccg ccggtcgaag gtaactcttt
     1681 agctgccaga atcgcccctg cgggtggcgt ttcaataaat gtcacatcgg tatgccagtt
     1741 gtcgttatcc ggcggattat cgttatgggt atccagcacg atgatctcgt caaccccttc
     1801 ggcatgaggg taaacagggt gaatatgcag ttcgccaaaa cgctgggcca gcgcgcgttg
     1861 ctgctgcggc gtaatagctt gatcgcgcag aaataccacc tgatagcgca gcaccgcatg
     1921 gtaaagctgt tcaaactgat tatcgcttaa cgggcgcgtc aggtcggcac ccgaaatttg
     1981 tgcgccgata tacggcccca gcggggtaat actcagacgt tcactcattg tacttctcca
     2041 tgccagggcg tcagacggcg ctgtaacgcg cgcagaccca gttctaaaag aaaggcgata
     2101 atcgcaatca ccgcgatccc cgccagtacc acgtcagtcg cgagaaattc acccgctgac
     2161 tgaaccataa atcctaaacc gcgtgtcgcg gcaatcagct ccgccgctac cagcgtagac
     2221 cagcccacac ccagaccaat gcgtaatccg gtgaggattt ccggcagcgc gccgggcaaa
     2281 atgacaaacc acagcacctg cgcacggctg gcacccagcg actgggcggc acgaatgcga
     2341 acctgctgcg cgcttttcac ccccgccagc accgacatcg ccaccggtgc aaaaatcgct
     2401 aaatagatca ataagatctt cgaggtttca ccaataccaa accagatcac catcagcggc
     2461 aaataagcca gcggcggcac cggacgataa agttcgatta ccggatccag aatgccgcgc
     2521 accgtcgggc ttagccccat ggcgatcccg accggaatac cgatcaccac cgccgcgagt
     2581 agcgccagca caatgcgcgt caggctggct gccagatgct gccacagcgt ggcgtccata
     2641 aatccctgcg gtccggcaat ggtgagcagt tttgccagta cttgttgcgg cggcggcaaa
     2701 aacagcgggc taatcagttg cagcgccgcc accgtccacc atacagtgag taaaaccgct
     2761 aacgtgccaa tgcttaaggt cacctgacgc gagagcggcc agcgccattt cagccgccgc
     2821 gaatgcagtt tttcattaat gagcacactc atgagaacgc ctcccgttgc tcaaatacgc
     2881 ggcttaaaac atattcgcgc atggcgatga attgtggatc ggacttgatg ctgcggctcg
     2941 actctcccgc aacaaagcgg cgagcaaagt tgagcggcag ccgctccagc acacggccag
     3001 ggccggatga aagcagaacc agttcagtcg ccataaacac cgcttcttct atatcgtggg
     3061 taatcaacag cacctgcttg cccgtctcct gccagagttt cagcagcaga gtttgcatct
     3121 ggtcgcgggt gaaggcgtcc agcgcaccaa acggttcgtc gagtaataac agctggggat
     3181 tcgccgccag cgcgcgggca atccccaccc gctgacgttg accaccggaa agctgccaga
     3241 tatagcgttt ttctgcgcct tccagcccca cttttttcag catctggtgc gcgatttcca
     3301 gtcgctgcat tttctctata cctgccaatt gcaggccgaa cgccacgttg tcctgtacat
     3361 tgcgccacgg tagtagccct tcattctgaa aaaccacccc gcgctctgct cccggtccct
     3421 caatacgctt acccgccagt tgaatgctgc catgctgata aggcacaaaa ccggcaatca
     3481 gattcagcag ggtggtttta ccgcagccgg acggccccag caccaccagt agctcgccgc
     3541 tttccagcgt caggttgata tcttccagtg ccggtttgcc gccataatcg gcatagagat
     3601 gagagatttg cagcatctgc gcctcctttt attgcacgaa gcgcgaggta acgtactggc
     3661 tgtaatcatt cgctacagcc gggaccttgc cctgctcttt caaaaactgc gcggtgtcga
     3721 tgattgcttt gttcaccggt ccggtcagtt ctgccgtttg ttgctgcggc gtcagatagg
     3781 tattcccctt caccagcccc ggaacgtcac cttccggcac gccgcttaaa cgcgccagtt
     3841 tgctgatgtt ttccggctgt ttcagccacg cgtctgggtt agcaatgtac ggttgctgag
     3901 catcgatggc gcttttagcg aacgctttca cgacctcagg atgtttctcg gcaaaatctt
     3961 tgcgcaccac ccagacgtcc agcgttggcg caccccactg cccgacctgt tcagaatcgg
     4021 tcaacacctt gccgtctttt tccagggcgt taaccgccgg tgcccagaca taagcaccat
     4081 caatatcacc ccgctgccat gcggcgataa tcgcgggcgg ctgcaggttc acaatctcca
     4141 cttgccctgg tttaatcccc cagtgtttca gcgccgccag caggctgtag tgggtggtgg
     4201 agataaacgg tacggcaatg cgtttgccaa tcaaatcttc gggtttgctg atagttttct
     4261 ttaccaccag cgcttcggag ttacccagtt ttgacgccag caagaagact tcaatcggca
     4321 cctgttggct ggctgcaacc gctaatgggc tggaaccgag gttgccgatt tgcacgtcgc
     4381 cagaagccag cgcccgcacg atgctggctc cgctgtcaaa cttacgccag tccacgctcg
     4441 ccccgctctc tttagcaaag gtgttgtcgg cctgagccac tttcgccggt tcggctgagg
     4501 tttgatacgc cacggtgacg ttcaccgcct gtgcctgaaa agcgatgaat gccagtgcgg
     4561 caagaagtgt gttacgcgat gaaattgcca tgattgtctg ctcccctgtc ttgttatggg
     4621 agcagtattc aggaataaaa acattcatta aaagaattag tcgttatcgc acagatgatt
     4681 ttattcttag caaaaaaacg gcaaaggaaa gat
//
LOCUS       DAZOZD010000289         4607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOZD010000289 DAZOZD010000000
VERSION     DAZOZD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.292"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..2163
                     /locus_tag="ACHUPB_003995"
     CDS             <1..2163
                     /locus_tag="ACHUPB_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0760663.1"
                     /translation="KGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKA
                     SGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMR
                     EMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGK
                     PAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLS
                     SVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVY
                     EIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYEN
                     VIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCF
                     GAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSAS
                     ELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKV
                     CSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLA
                     GNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLA
                     NIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVI
                     HEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2397..3680)
                     /locus_tag="ACHUPB_003996"
     CDS             complement(2397..3680)
                     /locus_tag="ACHUPB_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0760664.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3815..>4607)
                     /locus_tag="ACHUPB_003997"
     CDS             complement(3815..>4607)
                     /locus_tag="ACHUPB_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0760665.1"
                     /translation="QKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKA
                     ASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESS
                     PCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGI
                     SMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELR
                     SLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1094 a         1142 c         1194 g         1177 t
ORIGIN      
        1 aaaggcacta ttgcctggtc tattctctct tctcataata cgtccggaaa tatggataaa
       61 cttaaaatta agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag
      121 accgctaaag cgtccggaat ggaacgtttc ccgctgccgt atgtgctgac caactgccat
      181 aactcactct gcgccgtcgg cggcaccatt aacggtgatg accatgtttt tggtttatcg
      241 gcagctcagc gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat
      301 atgcgtgaga tgatggcagg cgggggcaaa atgatcctcg ggtcagacag ccacacccgt
      361 tacggtgcat tagggacaat ggcagtcggt gagggtggcg gtgagttggt aaaacagctg
      421 cttaatgaca cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa
      481 cctgcgccgt atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa
      541 aacggttacg tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct
      601 accgatttcc gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc
      661 tggcaaactg atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac
      721 tgccagctta accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc
      781 gccatcaaac caatgattgc gctgccgttc cacccgagca acgtgtatga aatcgacaca
      841 ctgaaccaga acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg
      901 cacggtaaag ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag
      961 caggggatta tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat
     1021 gcactgcgcg gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca
     1081 cagccggtgt ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc
     1141 gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac
     1201 aacggtttga gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca
     1261 gctaatgggc agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg
     1321 gcaaacggtg gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag
     1381 tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca
     1441 actcagcaac cgctgattta cgggccgaac attaaagact ggccggaatt gggtgcgctg
     1501 actgacaata tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac
     1561 gaactgattc cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag
     1621 tttaccctgt ctcgccgtga tcccggttat gttggcagaa gtaaagcgac tgctgagctg
     1681 gaaaatcagc gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag
     1741 cagattgctg gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtctat
     1801 gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt
     1861 ggcggtctgg cgaatattgc cgaggagtac gcgactaaac gctaccgttc taacgtcatc
     1921 aactggggga tgttacctct gcagatggcg gaagtgccaa cctttgaagt gggggattac
     1981 atttacatcc ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat
     2041 gtgatccatg aagatgcgcc ggtaacggaa attacgctct atatggaaag tctgactgct
     2101 gaagagcgcg agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg
     2161 taaaaagcgc catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc
     2221 gcatcttatc cgacctacga atcgcatcga atctgtaggc cagataaggc atttacgcag
     2281 catccggcac ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat
     2341 cgaaccgtag gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac
     2401 ttcttcgcct ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg
     2461 ttgtagttag tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta
     2521 ttacccgcaa acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa
     2581 ccttcgttga tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc
     2641 agcgagcggc ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg
     2701 aggaaaccgt agtacatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga
     2761 gtacgtgagt tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa
     2821 acgatatcca catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacgattc
     2881 gtttccagac ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga
     2941 atgttaacgt tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg
     3001 ttacctgcat ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg
     3061 ccattgtttt gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg
     3121 ctctggcagc tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg
     3181 ttgacgtcgt gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc
     3241 acatcaatcg gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca
     3301 tataccgttc cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc
     3361 ttgataattg ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc
     3421 gccgggccga caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct
     3481 gcagcacctg gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt
     3541 gccgacagaa tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg
     3601 ggcggggttg agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg
     3661 gaaactgaaa atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg
     3721 aagcctgctt ttttatacta agttggcatt ataaaaaagc atcgcttatc aatttgttgc
     3781 aacgaacagg tcattatcag tcaaaataaa atcattattt gatttcaatt ttgtcccact
     3841 ccctgcctct gtcatcacga tactgtgatg ccatggtgtc cgacttgtgc ccgagaagat
     3901 gttgagcaaa cttatcgctt atctgcttct catagagtct tgcagacaaa ctgcgcaact
     3961 cgtgaaaggt aggtggatcc ccttcgaagg aaagacctga tgcttttcgt gcgcgcataa
     4021 aataccttga tactgtgccg gatgaaagcg gttcacgacg agtagatgca attatggttt
     4081 ctccgccaag aatctctttg catttatcaa gtgtttcctt cattgatatc ccgagagcat
     4141 caacatgcaa tgttgtaggg atggcaattt ttacgcctgt tttgctttgc tcgacataaa
     4201 gatatccatc tacgatatca gaccacttca tttcgcataa atcaccaact cgttgcccgg
     4261 taacaacagc cagttccatt gcaagtctga gccaacatgg tgatgattct gctgtttgat
     4321 aaattttcag gtattcgtca gccgtaagtc ttgatctcct tacctctgat tttgctgcgc
     4381 gagtggcagc gaccggtttt gttgttatat ggccttcagc tattgcctct cggaatgcat
     4441 cgctcagtgt tgatctgatt aacttggctg atgccgcctt gccctcgtct atgtatccat
     4501 tgagcattgc cgcaatttct tttgtggtga tgtcttcaag tggagcatca ggcagccccc
     4561 tccttattgc tttaattttg ctcatgtaat ttatgagtgt cttctgc
//
LOCUS       DAZOZD010000290         4552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOZD010000290 DAZOZD010000000
VERSION     DAZOZD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.293"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="rutC"
                     /locus_tag="ACHUPB_003998"
     CDS             <1..268
                     /gene="rutC"
                     /locus_tag="ACHUPB_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0760666.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            276..1076
                     /gene="rutD"
                     /locus_tag="ACHUPB_003999"
     CDS             276..1076
                     /gene="rutD"
                     /locus_tag="ACHUPB_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0760667.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1086..1676
                     /locus_tag="ACHUPB_004000"
     CDS             1086..1676
                     /locus_tag="ACHUPB_004000"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0760668.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1687..2181
                     /gene="rutF"
                     /locus_tag="ACHUPB_004001"
     CDS             1687..2181
                     /gene="rutF"
                     /locus_tag="ACHUPB_004001"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0760669.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2202..3530
                     /gene="rutG"
                     /locus_tag="ACHUPB_004002"
     CDS             2202..3530
                     /gene="rutG"
                     /locus_tag="ACHUPB_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0760670.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3788..3961)
                     /locus_tag="ACHUPB_004003"
     CDS             complement(3788..3961)
                     /locus_tag="ACHUPB_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0760671.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
BASE COUNT          971 a         1164 c         1263 g         1154 t
ORIGIN      
        1 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
       61 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
      121 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
      181 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
      241 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
      301 cttatgctga tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
      361 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
      421 gcaccggcaa taatcccgac acgctggcag aagattacag tatcgcccag atggcagcgg
      481 aactgcatca ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc
      541 tcggtgcgct ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga
      601 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
      661 aacgattact gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct
      721 atcccgccga ctggatggcg gcccgcgcac ctcgccttga ggcagaagac gcgctggcac
      781 tggcgcattt tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg
      841 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
      901 atctgctggt gccatcagca tgttccagtg agcttcatgc cgccctgccc gatagccaga
      961 aaatggtgat gcgctatggc ggacacgcct gcaacgtgac cgaccccgaa acgtttaatg
     1021 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     1081 ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca
     1141 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc
     1201 cgataaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     1261 ctgtagaagg aaaagaacgt ctgcacccgg cgctttccag cggcaatcta caaaaaaccc
     1321 tgaccgcgcc cgtcaccgct atcgttgcct gggacagtga attttatgaa cggttaccac
     1381 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     1441 aaacagcgtt tcgcaacagc tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     1501 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     1561 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     1621 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     1681 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     1741 gcggtcaata tcatcaccac ggacggtcca tccgggcgcg ccgggttcac cgccagcgcc
     1801 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     1861 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     1921 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     1981 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     2041 tgccgcatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     2101 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     2161 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     2221 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     2281 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     2341 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     2401 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     2461 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     2521 cgaatatcag catcgcactg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     2581 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     2641 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     2701 cggcttcagc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     2761 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggttta attgtcgcct
     2821 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     2881 tcgtcagcca tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatagtc
     2941 aggcaatgat gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     3001 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     3061 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     3121 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     3181 ctgctgccgt catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     3241 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     3301 caggcgcaag gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     3361 tggtcgcagt aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta
     3421 cgttgggagg gatcggtaca gccacctttg gcgcgatttt actcaatgcg ttgttaagta
     3481 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     3541 gccggggtta cccggcggtg cagattgttg acaacgtacg cattgttcat gccggatgcg
     3601 gcgtaaacgc cttatccggc ctacaaaaca gtgcaaattc aatatattgc aatcttctgg
     3661 taggcctgat aagcatcgcg catcaggcaa gcttacattt gtcatcagta tcaaccaccg
     3721 ggtaaccggc ggtgatggct ttttgacgag ctacagacaa aaatgcattc gtcattgatt
     3781 agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
     3841 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
     3901 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
     3961 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
     4021 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggt agacccgcgt
     4081 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
     4141 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
     4201 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
     4261 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
     4321 ggagtggtta gaaatggcta aagttctggt gcttttacat agctaaacac gtacattcaa
     4381 ctacctgtta ataacaggct ttatcaggtg tagttgggct tgttccaacc cctatgacta
     4441 tgcaatccta cacagattct atgcagtatc ggtatagtcc ctttttatcc taacttattg
     4501 aaatatatag agaatctgtg aagccccttt tcagggaact atgcagggtg tt
//
LOCUS       DAZOZD010000291         4514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOZD010000291 DAZOZD010000000
VERSION     DAZOZD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.294"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..190)
                     /locus_tag="ACHUPB_004004"
     CDS             complement(<1..190)
                     /locus_tag="ACHUPB_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0760672.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIG"
     gene            complement(193..912)
                     /gene="sanA"
                     /locus_tag="ACHUPB_004005"
     CDS             complement(193..912)
                     /gene="sanA"
                     /locus_tag="ACHUPB_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0760673.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(1062..1946)
                     /gene="cdd"
                     /locus_tag="ACHUPB_004006"
     CDS             complement(1062..1946)
                     /gene="cdd"
                     /locus_tag="ACHUPB_004006"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0760674.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(2076..2771)
                     /locus_tag="ACHUPB_004007"
     CDS             complement(2076..2771)
                     /locus_tag="ACHUPB_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0760675.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(2768..3166)
                     /locus_tag="ACHUPB_004008"
     CDS             complement(2768..3166)
                     /locus_tag="ACHUPB_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0760676.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            3405..4352
                     /gene="dusC"
                     /locus_tag="ACHUPB_004009"
     CDS             3405..4352
                     /gene="dusC"
                     /locus_tag="ACHUPB_004009"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0760677.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         1182 a         1157 c         1175 g         1000 t
ORIGIN      
        1 agccgatata tgccaccata taagcgaaaa agagtccggg attttttgct cgccagacat
       61 acaccggttc cggtgcattt ttaacccgtt tatcaatcgc gcctgcgcgc cagcctttcc
      121 atgcttcgcg gaaacaaaat atcgggataa ggaaaaaaac ggcaacgaca aaaaactgct
      181 gtacatccat aactactttc cttgtttctt ttgtaattca agtaactgtt cgggtgtgac
      241 ggcgggatac ccctgcgcat cttccggcac ctggtgcata gccggaatag ggaccagcgg
      301 ccctaaaaaa cgcggttcac gtctaaaaat ataaaggtca gccagcgcac cgaaacgggc
      361 ggcaaattcg cgaatacgta ctgacagcat atctttcggt gacggtacgg cataacactg
      421 agcctgaatc cccatatgca gcgcaataaa taatgcccgc tcacagtgga aacgttgggt
      481 gataataatg aaatcattag tatcgaacac tttgcgtgta cgcacgatgg aatccagcgt
      541 acgaaagcct gcgtaatcga gaacaatatc tgatgggtcg acaccagcag cgattaaatc
      601 tttgcgcatg gtcatcggct cattataact ttgcaatgcg ttatcgccgc tcagtaatag
      661 ataatttacc ttaccgctgt tataggcatt aatcgctcct tgaatgcggt agcgataata
      721 ctgattaatt acgccagtac gataatattt tgctgtgccg agcaccacgc cgacctggcg
      781 gtaggggaga tcctgcaatt cgtcgtagat ataaggcgcg gttttccagc tcatccagcg
      841 atcgaggccg agcacagtca acagcagcaa gccgatcagg actaacaggc tgaggaacac
      901 gcgctttaac atgcaggaga ctcgatctaa ggaaaatttc catcaggcta ctttacccgc
      961 tggttttgcg caagaaacgg caaactgttt gccgggaatt tcgacaccaa gagagggctg
     1021 gcaagccgga caaggcgttc acgccgcatc cggcaccagg cttaagcgag aagcacgcgg
     1081 tcgatactgt gacagccgag agctttcagc gttgcggagg tggcatccca ctgaatcaac
     1141 ggcgcatcgg ctttttctgc cagaaccgcg cgctggatat ccgggtaatc ataacccttg
     1201 agattcaaca gaattaacgc cccctgcaac ggtggcagag tcgggttgaa tgcggcgttt
     1261 tcagcgtagc tgccgctgaa aatacgaccg tctttacatt ccagcgcgac accgcttggc
     1321 gacttactgt aaggcatgtg cgaacggttt gccgccgcaa tcgctgcctg agaaagcgca
     1381 tcacccgtca gcgcatagcc gtgatcctgt tcgtccatca gcagcgtttt aatctccaga
     1441 tctttcggcc caaaggcatc cggcagatag tcacgcagcg cgtgtgcctc gcggcccggc
     1501 agatgaatac gcagatccag accgctgttc agttcattca taaactgacg gcagtgacca
     1561 caaggcgtat agttaacggt gatggctgca agcgcttttt caccactcaa ccaggcgtgg
     1621 ctgatcgcgc tttgttcggc atgaacggtt tgctgcattg ttgcaccaat aaattccata
     1681 ttggcaccga aataccaggt tccgctcaca ccgcgcgcaa ttgcgccaac attaaaattc
     1741 gacaatggcg tacgcgcaca ggccgccgcc agtggaagta gtgcgaatgc cagcgcgtct
     1801 tcgtccagcc ccgttgcgct cttcagcgat gagacttgct ccccggtcaa caaagcgggg
     1861 aagtacttgt ctgccagaat aggttccagt gcagattgca agttatccgc aagttgggca
     1921 aaagcggttt gaaaacgtgg atgcatggcg ttgcctcata atcatgtaat gggataacag
     1981 tttacgaacc cgttatggct ttatatgtga tctgaatctc attaattatg caacgcgtga
     2041 aacttacatc aaaaatgcgc gacgcatcgc aaattttagc ccattactgc cagaataatc
     2101 gggaaaagga acggtgcgat cagcgaagta attatcccgc ataacaccag cgccagcgaa
     2161 ctaaatgcac cttcctgata atccagctcg gcgcagcgcg ccgtaccgag ggcgtgcgag
     2221 gctgtcccca ttgccagacc gcgcgcagct ttggtacgaa tacgcatcgc attaagcaat
     2281 gtatggccaa ataccgcgcc gaggatgccg acgaaaatca cgcaaactgc gctgattgcc
     2341 ggaataccgc caatactgcc gccaaccgcc attgcaatag gcgtggtgac tgatttcggc
     2401 aggattgacg cggcgatttc cggtgaagcg cccatcaaca atgccacgga agtccccgtc
     2461 accatcgcaa ccacgctgcc gataaaacag atggtgatga tcgatttcca gcgcgcgcgg
     2521 atctggtgta gctgctcata taaaggatag gccagcgcga cgaccgccgg ttgcagcaga
     2581 tcgtttagca cttcgctacc tttaaagtag ctgtcgtaag agatgccggt cagcattaaa
     2641 aaaggaatga tcaccaccat tgctaccagc aacgggttaa gcaggggaaa cttataccgt
     2701 gccgccagtt tgcgggcggc aaaaaagaca atcaacgtca gcggtaatga ccaccagata
     2761 ttcgccatca ttcttctgat cctttctgac ctacgacttt acgttcaccg tgtaccagtt
     2821 gtgaactcca gctcaccacc agaaaaacca ccagcgtact gactgcacag gaaaccacta
     2881 ccgggccaaa ctgtgcgcgg agcaaatcaa aatattgcat gacgcctacg ccaatcggca
     2941 caaacaatag cgccatatag cgaatcagta cgtagcaccc cggattgacc cattttgccg
     3001 gaagaatttg caaggccagc aggacaaaca ggatcagcat cccgatgatg ctgcccggaa
     3061 tggttaccgg tagcagggaa gcaatgaaaa tgcctgcata caggcaggca taaatcagga
     3121 cgaaagcgcg taaatattgc cagataatgt tcagtgtctt actcataatc aacaccccag
     3181 atgagatgga ttcatcatac aattaattcg taaaacgtgc taccgatcac attaagaaat
     3241 cagcgcttat tttttcgctc cccggcatta cgcaatgcgc aacgaactca ggcatacatc
     3301 aagcatagag agggattaca gtttgcagca atccctcttt gcagaccttt aatccgttga
     3361 ccataggcgc taacatagcg cctcattttt ttccgggtga agatatgcgt gtgttactgg
     3421 ctccgatgga gggtgtgctt gactctctgg tgcgagaatt gctgaccgaa gttaacgact
     3481 acgatctgtg catcaccgag tttgtccgcg tggtggatca actgctgccg gtaaaagtct
     3541 ttcatcgcat ttgccctgag ctacaaaacg ccagccggac accatccggt acgctggtgc
     3601 gcgttcagtt gttaggtcag ttcccacaat ggctggcaga gaacgccgcc cgtgcggtgg
     3661 aattaggttc ctggggcgtg gatctcaatt gcggctgccc gtcgaaaacg gttaacggca
     3721 gcggcggcgg ggcgacgtta ctcaaagatc ctgaactcat ctaccagggt gcaaaagcga
     3781 tgcgtgaagc tgtaccggcg catttgcccg tcagcgtgaa agtgcgtctg ggctgggaca
     3841 gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca ggctggtgct acggagctgg
     3901 tggtgcatgg acggacaaaa gagcagggtt accgcgcgga gcatattgac tggcaggcga
     3961 ttggcgagat tcgccagcgg ctgaatattc cggtgattgc caacggcgaa atctgggact
     4021 ggcagagcgc gcaacaatgc attgcgatca gcggctgcga cgcagtgatg attggtcgcg
     4081 gggcgctcaa tattcccaac ctgagccggg tagtaaaata taacgaaccg cgaatgccgt
     4141 ggccggaggt ggttgctttg ctgcaaaaat atacccgtct ggaaaagcag ggcgataccg
     4201 ggttatatca cgttgcgcgg attaaacagt ggttgagtta tttgcgtaaa gaatacgatg
     4261 aagcaacgga attatttcag catgttcggg tgttgaataa ttcccctgat attgcaaggg
     4321 ctattcaggc aattgatatc gagaaactct aaaaaaccat agtgatgttt tttgacaatg
     4381 tgttagctct ttatcaatag gccgcacggt tgtttggtgg cgtcaaaatt gccgaggcgt
     4441 tccctgaagg ccggggcagc ccacatggag gtgggctgag ggcgcgtttt acagggatgt
     4501 tacctcgcgc ccga
//
LOCUS       DAZOZD010000292         4497 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOZD010000292 DAZOZD010000000
VERSION     DAZOZD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.295"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..476
                     /locus_tag="ACHUPB_004010"
     CDS             <1..476
                     /locus_tag="ACHUPB_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR0760678.1"
                     /translation="LHAYKQNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNK
                     GEARNALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKR
                     KGMKINAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(1002..2177)
                     /gene="senB"
                     /locus_tag="ACHUPB_004011"
     CDS             complement(1002..2177)
                     /gene="senB"
                     /locus_tag="ACHUPB_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR0760679.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2246..>4497)
                     /locus_tag="ACHUPB_004012"
     CDS             complement(2246..>4497)
                     /locus_tag="ACHUPB_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0760680.1"
                     /translation="KLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKT
                     TSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFGR
                     VNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINAL
                     AGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVM
                     AAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSARTYE
                     NKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQNRS
                     DALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFAPSG
                     QQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDKEGG
                     FNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETWQAG
                     FNIDTRDLLVEQDALRFKALAYHSRIQNYIYSESYLVCSGGRKCSMPEVIGNDWEGIS
                     DEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYFGAG
                     DMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQDLPQ
                     IPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSARGRT
                     WIFGGDIRF"
BASE COUNT         1141 a         1052 c         1048 g         1256 t
ORIGIN      
        1 tgctgcacgc ttacaaacaa aatgggttgg ctaaagctct gagagagatt ggccgtatag
       61 aacgaacact gtttatgctg gactggtttc gggatccggc attgcggcga cgtgtccagg
      121 ccgggctgaa caaaggagaa gcccgtaatg ctctggcccg tgctgtattc atgcatcgat
      181 tgggggagat tagagatcgg aaaccggaaa atcaaagtta tcgcgccagt ggattgactt
      241 tattgacggc ggctatttcc ttatggaaca cagtttacat ggaaagagcg gttgatgctc
      301 tgaagcgtaa gggcatgaaa ataaatgctc aactattatc gcatttatca ccattaggtt
      361 gggaacatat caatctgaca ggcgattata tctggaaaaa cagccggata ccgacttcag
      421 gtaagtttcg tcgtttgaga tcagttaaga tcgacaaggt caaaaaacaa ccttaacgta
      481 caataatttt cgatatccaa actgacccca agtataaaaa acagcctgtc acggtataaa
      541 aatcgaccat gcagaaatta tgctggttat tttaccgctg ctggtccctc attcagtgac
      601 tgccccaggg gttttcatgc tctccttata gtacaataat ttccatatcc aaactcaccc
      661 ctactttgac ttttatcata gtcaaaacct cttaacgtga gttttcgttc cactgagcgt
      721 cagccctcta aaactactga ataatcagtt agaaaacaaa caatcggaaa aatctggggt
      781 tccggcctag aaccccatga acaggcatag ctgtgatttc atttaattat tggcacaaat
      841 cagttgatcg tacaatgctg tgcctgtctc gttttctgga gattcctcag cctcatggag
      901 cggcgtaaca ggcgtaccgg caggacagag atgtttgctt ttcctagtcc ggccagttta
      961 gtggaataac ggagttttta tcccatgttt tttccccccg atcacgactt tttcaaatca
     1021 atgctcagaa tacgttcttt agctccttcc acaaacacat caggatccca atcgcggtta
     1081 aagccttctt tgatgccaat gtaaagggta tccatatcgt ccgacaacac catctggttt
     1141 gggtagaccg gcatgttaaa ttcacgggtg atgctgtatt tggtggcatc cactacgctg
     1201 atggtgccgc tagtgaagtt ggtgacatac agttcgttat atttcggatt gtatttcacc
     1261 gcgttagctc cggctcctgt gtgcacatag ccaagctgtt taccgtcgcg taagtcaaaa
     1321 atatagagtc catcttttgt accggaacga tcgtcatggt cgaataccac cacaaaggca
     1381 cggtcagaag cggagtccac cgccagattc tgcacacttg tgatttgtgg tctcggatct
     1441 ttcaacggga tggtacgcag cagcgtctga tttttcgcat caatttcgtt gatgtaatct
     1501 cttccgccaa cgtagatttt ctgtgttttt tcatccatcg tcagaccata cgctgtacct
     1561 ttgtaagcat ccttaacctc gccttttaag gcaagcgtcg tcccgtcgag cattaacagc
     1621 ttatgcagag gcttcatgcc ctcggccgtt ttcagcatat tgctataact cacaaacagg
     1681 gtatcggaat cctggctgta aaccatatgg cgaaaatgct caaaacgcga atctgcagcg
     1741 tttgccgctt taaaacttaa cctgtcgctg gttcttacca gtttgccggt tggggtgtca
     1801 aacatactga tacgcaggga cgctgactgg gtgtgtccga tatagagctg atgtttatcc
     1861 tgatttatcg ccagcgaaaa tgcccggtat ggcatcgtgt atttattttc gactttcagc
     1921 gaatctgaat taaacgaata cagatagcct ttattggctt cacgatttac acgatcggtg
     1981 actgctgcaa acaccttttt actggcatta tcaaacgcca gttcatagac gccttcatca
     2041 ggggactgaa ggtaattcgt tttcagggca aatcgcgaaa gttcgcgttc aggtggatcg
     2101 atagccatgg ccatcgtggg taaaaatagt gaaattaaca ggtatagcgg tgctttggat
     2161 aaagtgaaaa tattcatttt tcgttcctgc aataaaattg acataagaga gaaaacgtat
     2221 tgtctgaacc tagcggaaat tgtcatcaga aacgaatgtc cccgccaaat atccatgttc
     2281 tgccacgcgc ggaattggct gggtgggtct cgtcaccaag acctggcatc atgttgagct
     2341 tattcagcgc ctccgaataa tctctgttca tcaggttttg taccgaaagt ttcagtgtta
     2401 ggttgcggtt gtactcgtaa gtaccataga gatcgataat cgttgggatc tgcggcaaat
     2461 cctgtttgat tattgcgccg gtatgttcgt cctgctcaaa atcaggcgac aggcgacgag
     2521 ccttgcctgt gtattttatg atagtgccca gggtcaacgc gttatcgaag aagcgaacac
     2581 cagtatccag cgtcatgtat tttctgggca gttcggtcat atcccctgcg ccaaagtagg
     2641 tgctggcgat ggaggttggc tggtctgttt gctgctggct gaaagagagt cggccaaaag
     2701 caaaacctgc atcataatcc atctccagtt cgaagccctt tgcgataacg tcgcttgccg
     2761 agttaacgta gatgtacata ttgtcgctgt attcatcgct aatgccctcc cagtcattgc
     2821 caatcacctc aggcatactg catttacgac ctccagaaca aaccagataa gactcgctgt
     2881 agatgtagtt ctggatcctg ctgtggtacg ccagagcctt aaagcgcagg gcatcctgtt
     2941 cgaccagtaa atctctggta tcaatgttaa aacccgcctg ccaggtttct gcacgttcag
     3001 gcttcaggaa tgggttcatg gatgcgcctc ctgaattaga gaagaacatc tcctggatgt
     3061 tcggggcgcg catggatttg ctgtagccaa tgaacggctg aagccatggt gttacctgag
     3121 cagaaagctg aactgaaggg ttgaagccac cctctttatc gtcaatatcg tagctaccct
     3181 gtggcacgca gataacgcgt gaatcgcagg cgggcttgta ccctgtgatc ctgttacgtg
     3241 tgtagttgag attgaaatct gcctcccaga tgccgcgcgt aaccttcagc ccggtataca
     3301 gcgctgaaat atcctgctgg ccggagggtg cgaacggatt gttctcgatc gattcctgat
     3361 tcgctttcgg gtcgcccgcc gctgagtgaa tggtccggtc atagcgggta cgcatcagtt
     3421 tgctgcccag cataaactcc aggtcattgt ccgcgacagt gaaccggctg gtattgttga
     3481 tatccagcgc gtcagaacga ttttgcgctg aggttttgta gaaagtatac agcgagccat
     3541 cacgatactt ttgattaccg cgactggtac tggccgttac gttaaagtca attaattcag
     3601 aaaaaggggt gtaatgatat ttaatgtaat agtcgtcact ggttatatca cgacgtgtaa
     3661 atttattttc ataggttcga gcggaaagtt cgaagctgtt aaattcgtct ggtctgatat
     3721 ccattttata cagttgggat ttggggttct gcttcatata tttatcataa ccaaactctt
     3781 tgctgttaat tccagaacca tttgagaaat tagagtacac agaactgccg ctcacagcag
     3841 ccataacgcc aatgcttccc gtatccgtaa aagcgtcgct ttttccacca agggcgatca
     3901 ttccgctgcg tcccagccca ttactaccga cagagaaacg tgaacgaagg ccatatgtat
     3961 taccgttaaa tattacatcg tcaacgccaa tagtacgcat atttgcacta ccggcaaggg
     4021 cgttgatccc ttcagagcca ctgctgtcac cgcgtgtaac atcaactgct accagtaagt
     4081 taggatctat aagtacgcca gccatattgt tagttgaacc atgcgtcgtt gttccggagg
     4141 tagaggttcc gtaaaaactc tgggtaatac catcgaccat agtgtttaca cgaccaaatc
     4201 cgctcatgcc tcgaatattc acactgattg ctccctgacc aggatctatt tgagtataag
     4261 taccaggcat actacgcact gttgcgtcaa gtgattgcag gtttttgtcc gttgtccggg
     4321 aactggtcgc gcctgttttc tcgagagctt cctgctcact ggtcgtcttc ccgacagaaa
     4381 cgctgagcgg actgaattct gccgctcctt ttttgtcgga attagttttt tcactgatgg
     4441 attgtgagta agcaccgcag ctgagcaata atatgcctga gccgatagcc agtttta
//
LOCUS       DAZOZD010000293         4482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOZD010000293 DAZOZD010000000
VERSION     DAZOZD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.296"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHUPB_004013"
     CDS             complement(7..1068)
                     /locus_tag="ACHUPB_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0760681.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHUPB_004014"
     CDS             complement(1065..2462)
                     /locus_tag="ACHUPB_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0760682.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHUPB_004015"
     CDS             2617..3615
                     /locus_tag="ACHUPB_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0760683.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHUPB_004016"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHUPB_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR0760684.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAZOZD010000294         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOZD010000294 DAZOZD010000000
VERSION     DAZOZD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.297"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUPB_004017"
     CDS             complement(<1..105)
                     /locus_tag="ACHUPB_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760685.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUPB_004018"
     CDS             complement(173..2122)
                     /locus_tag="ACHUPB_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0760686.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUPB_004019"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUPB_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0760687.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUPB_004020"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUPB_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0760688.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUPB_004021"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUPB_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0760689.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1314 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZOZD010000295         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOZD010000295 DAZOZD010000000
VERSION     DAZOZD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.298"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(21..1967)
                     /locus_tag="ACHUPB_004022"
     CDS             complement(21..1967)
                     /locus_tag="ACHUPB_004022"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0760690.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLGLIP"
     gene            complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUPB_004023"
     CDS             complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHUPB_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0760691.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2369..3076
                     /locus_tag="ACHUPB_004024"
     CDS             2369..3076
                     /locus_tag="ACHUPB_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0760692.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="ACHUPB_004025"
     tRNA            3182..3257
                     /locus_tag="ACHUPB_004025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="ACHUPB_004026"
     CDS             3455..>4409
                     /locus_tag="ACHUPB_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0760693.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZOZD010000296         4408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOZD010000296 DAZOZD010000000
VERSION     DAZOZD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.299"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUPB_004027"
     CDS             complement(<1..590)
                     /locus_tag="ACHUPB_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0760694.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUPB_004028"
     CDS             complement(583..993)
                     /locus_tag="ACHUPB_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0760695.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUPB_004029"
     CDS             complement(999..1532)
                     /locus_tag="ACHUPB_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0760696.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="ACHUPB_004030"
     CDS             complement(1510..2598)
                     /locus_tag="ACHUPB_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0760697.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="ACHUPB_004031"
     CDS             complement(2595..3986)
                     /locus_tag="ACHUPB_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0760698.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..>4408)
                     /locus_tag="ACHUPB_004032"
     CDS             complement(4033..>4408)
                     /locus_tag="ACHUPB_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760699.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1025 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgtt
//
LOCUS       DAZOZD010000297         4404 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOZD010000297 DAZOZD010000000
VERSION     DAZOZD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.300"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHUPB_004033"
     CDS             complement(139..3654)
                     /locus_tag="ACHUPB_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0760700.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="ACHUPB_004034"
     CDS             complement(4106..>4404)
                     /locus_tag="ACHUPB_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0760701.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1127 c          905 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAZOZD010000298         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZOZD010000298 DAZOZD010000000
VERSION     DAZOZD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.301"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            147..3239
                     /gene="ebgA"
                     /locus_tag="ACHUPB_004035"
                     /pseudo
     CDS             147..3239
                     /gene="ebgA"
                     /locus_tag="ACHUPB_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            3236..3685
                     /locus_tag="ACHUPB_004036"
     CDS             3236..3685
                     /locus_tag="ACHUPB_004036"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0760702.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKMVLKVTIEDGYFHNK"
     gene            3748..>4330
                     /locus_tag="ACHUPB_004037"
     CDS             3748..>4330
                     /locus_tag="ACHUPB_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0760703.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
BASE COUNT         1044 a         1167 c         1089 g         1030 t
ORIGIN      
        1 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgtcactttt
       61 tagtaaaaat tttactaaac tccccagcaa ttacacaaac taccatcacc atgaatggtt
      121 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
      181 aaaaccgact tgcgccacgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
      241 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cggtcagtgg aatttccact
      301 tttttgacca cccgctgcaa gtgcctgaag ccttcacctc tgagttaatg gctgactggg
      361 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
      421 acgaaggttt tccgttcccc atcgatgtgc cgtttgttcc cagcgataac ccaaccggtg
      481 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
      541 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
      601 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
      661 tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta cgtggaagac caggatatgt
      721 ggtggtcagc ggggatcttc cgcgatgttt atctgatcgg aaaacaacta acacatatta
      781 acgatttcac cgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
      841 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
      901 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
      961 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     1021 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtac
     1081 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     1141 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     1201 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaac atcaactccg
     1261 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     1321 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     1381 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     1441 ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     1501 atggctgtaa catccgcgcg atgtaccacg cagcgaaggc gctggatgac acgcgactgg
     1561 tgcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     1621 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     1681 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     1741 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     1801 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     1861 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggaccgggcc
     1921 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgcgggat ctgactcgcg
     1981 gcgagttgaa agtcgaaaat aaactgtggt ttaccacgct tgatgactac cccctgcacg
     2041 cagaggtgcg cgccgaaggt gaaacgctcg cgacgcagca gattaaactg cgcgacgttg
     2101 cgccgaacag cgaagccccc ttgcagatca cgctgccgca gctggacgcc cgcgaagcgt
     2161 tcctcaacat tacggtgacc aaagattccc gcacccgcta cagcgaagcc gggcattcta
     2221 tcgccactta tcagttcccg ctgaaggaaa acaccgcgca gccagtgcct ttcgcaccaa
     2281 ataatgcgcg tccgctgacg ctggaagacg atcgtttgag ctgcaccgtt cgcggctaca
     2341 acttcgcgat caccttctca aaaatgagtg gcaaaccgac atcctggcag gtaaatggcg
     2401 agtcgctgct gacccgcgag ccaaagatca acttcttcaa gccgatgatc gacaaccaca
     2461 agcaggagta cgaagggttg tggcagccga atcatttgca gatcatgcag gaacatctgc
     2521 gcgactttgc cgtagaacag agcgagggtg aagtgttgat catcagccgc acggttattg
     2581 ccccgccggt gtttgatttc ggaatgcgct gcacctacat ctggcgcatc gctgccgatg
     2641 gccaggttaa cgtggcgctt tccggcgagc gttacggcga ctatccgcac atcattccgt
     2701 gcatcggttt caccatggga attaacggcg aatacgatca ggtggcgtat tacggtcgtg
     2761 gaccgggcga aaactacgcc gacagccagc aggctaacat catcgatatc tggcgcagca
     2821 ccgtcgatgc catgttcgag aactatccct tcccgcagaa caacggtaac cgtcagcatg
     2881 tccgctggac ggcactgact aaccgccacg gtaacggtct gctggtggtt ccgcagcgcc
     2941 caattaactt cagcgcttgg cactatacct aggaaaacat ccacgctgcc cagcactgta
     3001 acgagctgca gcgcagtgat gacatcaccc tgaacctcga tcaccagctg cttggcctcg
     3061 gctccaactc ctggggcagc gaggtgctgg actcctggcg cgtctggttc cgtgacttca
     3121 gctacggctt tacgttgctg ccggtttctg gcggagaagc taccgcgcaa agcctggcgt
     3181 cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca cagcgagaat aagcaatgag
     3241 gatcatcgat aacttagaac agttccgcca gatttacgcc tctggcaaga agtggcaacg
     3301 ctgcgttgaa gcgattgaaa atatcgacaa cattcagcct ggcgtcgccc actccatcgg
     3361 tgactcattg acttaccgcg tggagacaga ctccgcgacc gatgcgctat ttaccgggca
     3421 tcgacgctat tttgaagtgc attactacct gcaagggcag caaaaaattg aatatgcgcc
     3481 gaaagagaca ttacaggtag tggaatatta tcgtgatgaa actgaccgtg aatatttaaa
     3541 aggctgcgga gaaaccgttg aggtccacga agggcaaatc gttatttgcg atatccatga
     3601 agcgtatcgg tttatctgca ataacgcggt caaaaaaatg gttctcaaag tcaccatcga
     3661 agatggttat ttccataaca aataacaact acggcggcaa aaggagtttg ccgccaccgc
     3721 taccctactc attttcggag atgtgttatg tctgatacca aacgtaatac aatcggcaaa
     3781 ttcggcttgc tctcgctgac ttttgccgcc gtttacagct ttaacaacgt tatcaataat
     3841 aatattgatc ttggactggc ctcggcaccg atgtttttcc tcgcgacgat tttttatttt
     3901 attcccttct gtctgatcat cgcagaattt gtttcgttaa ataaaaactc agaagccggt
     3961 gtctacgcat gggtaaaaag ttcgctgggc ggacgttggg catttattac tgcctatacc
     4021 tactggttcg taaacctgtt ctttttcacc tcactgttgc cgcgcgttat tgcttatgct
     4081 tcgtatgcct tcctcggata cgaatatatt atgacgccgg ttgccaccac cattatcagt
     4141 atggtgctgt tcgccttctc cacctgggtt tccaccaacg gggcgaaaat gttggggcca
     4201 attacctccg tcacttcaac gctgatgctg ctgttaacgc tctcctacat tttactggca
     4261 ggtacggcgc tggttggcgg cgtacagcct gctgacgcca tcaccgttga cgcgatgatc
     4321 ccgaacttca
//
LOCUS       DAZOZD010000299         4273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOZD010000299 DAZOZD010000000
VERSION     DAZOZD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.302"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            147..299
                     /gene="hokA"
                     /locus_tag="ACHUPB_004038"
     CDS             147..299
                     /gene="hokA"
                     /locus_tag="ACHUPB_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0760704.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(276..347)
                     /locus_tag="ACHUPB_004039"
     CDS             complement(276..347)
                     /locus_tag="ACHUPB_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760705.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(487..699)
                     /gene="cspA"
                     /locus_tag="ACHUPB_004040"
     CDS             complement(487..699)
                     /gene="cspA"
                     /locus_tag="ACHUPB_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0760706.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(980..1270)
                     /locus_tag="ACHUPB_004041"
     CDS             complement(980..1270)
                     /locus_tag="ACHUPB_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR0760707.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1704..2414
                     /locus_tag="ACHUPB_004042"
     CDS             1704..2414
                     /locus_tag="ACHUPB_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0760708.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2464..3438)
                     /gene="ghrB"
                     /locus_tag="ACHUPB_004043"
     CDS             complement(2464..3438)
                     /gene="ghrB"
                     /locus_tag="ACHUPB_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0760709.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3542..4201)
                     /locus_tag="ACHUPB_004044"
     CDS             complement(3542..4201)
                     /locus_tag="ACHUPB_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR0760710.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1056 a         1030 c         1043 g         1144 t
ORIGIN      
        1 tgcttgagac tgtttgtctc aggtattcac cgaaaggcag acagagaaaa gccccacctg
       61 actataaatc aaagtgaggc taccctatgc ctgaacacca taaggttagc ctcttactcg
      121 ttggaaatca acacaggagg ctgggaatgc cgcagaaata tagattactt tctttaatag
      181 tgatttgttt cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc
      241 atattaaaca ggagagttat gagctggcgg catttttagc ctgcaatttg aaagagtaag
      301 agtctttggc gggaagttat tcccgccttt cttacggcgt tgcgcattct cattgcaccc
      361 aaatttattc ttcacaaaaa taatactaaa ttttattacg cgatcgatta tttatttcct
      421 gaaaaacaaa taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag
      481 cagagattac aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt
      541 gaaggacact ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa
      601 gtgtacgaac acatctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc
      661 gttgaaccat tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt
      721 aagtgtgcct ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc
      781 actacgaggg gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg
      841 gtctgtacgt caaaccgttg atgtgcatta agccacgcat tggcgggtga tgcaacaatt
      901 atttttcata tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag
      961 caataaagtg gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa
     1021 tcaaacgcat caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct
     1081 ttaccatggc gactgatacg cccaaaaccc gggcgaaatc atcgattttt aaccctgtcc
     1141 cttttcgtaa ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct
     1201 gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt
     1261 tatattccat tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca
     1321 ttaagaatag tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg
     1381 attcgattca gcctgtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg
     1441 atatatctct taccactcct gttttgtaaa gatttcgaaa atctaacctt ctgcgaaggc
     1501 gtaaatctct ggctacaaaa aaggcaacgg cactatcatt ttttaacaat gaaacaacaa
     1561 attaaccttg tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc
     1621 tgcaagcctg tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt
     1681 ttttgggtga aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc
     1741 ctcttgcggc gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc
     1801 agcgtaaagc gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac
     1861 caaccctgac tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc
     1921 tgtatggtta ttctcagcag gtgaatcagg cgatggattc tggtctgcgc ccggtcgtag
     1981 acagcgttaa cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg
     2041 aaatgaatgg ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg
     2101 atgctgcgca ctctgcgcta aaacagagcg atgacctgaa accggtattt gatcaggcat
     2161 tcaccaaagt ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa
     2221 ctttcacgca acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga
     2281 gctttgtcgc aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac
     2341 tgattgcgcc attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta
     2401 ctgcaaccca ataaagagta aaagcccgag cgattatcgg gctttacttc acgcagtcgc
     2461 ggcttagtcc gcgacgtgag gattcacaca gttcttctca acctttcctt gtaacgcatc
     2521 aattaaatta tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc
     2581 aatatgcggt actgcgacga cgttggccat tgagagcaac ggcgaatcta cggacagtgg
     2641 ctcttgttcg aagacatcaa gcccggcggc gtgaatttcc cctttctgta atgctgcgat
     2701 cagtgcattt tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt
     2761 cattttggcg aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat
     2821 caggcaaacg aaatctgact cttgtaacag tgtatccaaa tcgcagtagc gggcgttgaa
     2881 gcgttcttct gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc
     2941 aaagtgcgca cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt
     3001 tttatggtga acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc
     3061 ttttacccgt tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag
     3121 cgtatcggcg acggtttctg ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc
     3181 ggtaagcgca tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcatgcag
     3241 tttcggcatt ttttccagca atgcggcatc aacattctcg tttgaaccca gtaaaccttc
     3301 agcttcggca aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg
     3361 gtgaacggtg aaatgctctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag
     3421 gataacggac ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa
     3481 atatgatgaa ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg
     3541 attacagcgg gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc
     3601 tgttgctggc gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca
     3661 cgccctgggt gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt
     3721 tcaggtcgtg accccccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat
     3781 actctgtcag caccattgcc acgccggtca gggtgttagc gcctgccggt ttcagggtcg
     3841 cgctgctgct gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc
     3901 gggttacgct aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca
     3961 tgtaataacc cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt
     4021 taccgcgatc tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca
     4081 gacctgcgcc gatagcagat ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc
     4141 agccagatac cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca
     4201 ttgttaatcc ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc
     4261 cgcgtgaaca tga
//
LOCUS       DAZOZD010000300         4271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOZD010000300 DAZOZD010000000
VERSION     DAZOZD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.303"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(84..902)
                     /gene="cof"
                     /locus_tag="ACHUPB_004045"
     CDS             complement(84..902)
                     /gene="cof"
                     /locus_tag="ACHUPB_004045"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0760711.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            1002..2702
                     /locus_tag="ACHUPB_004046"
     CDS             1002..2702
                     /locus_tag="ACHUPB_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR0760712.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQEQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2767..3462
                     /gene="queC"
                     /locus_tag="ACHUPB_004047"
     CDS             2767..3462
                     /gene="queC"
                     /locus_tag="ACHUPB_004047"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0760713.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(3514..3912)
                     /gene="fadM"
                     /locus_tag="ACHUPB_004048"
     CDS             complement(3514..3912)
                     /gene="fadM"
                     /locus_tag="ACHUPB_004048"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0760714.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4006..>4271)
                     /locus_tag="ACHUPB_004049"
     CDS             complement(4006..>4271)
                     /locus_tag="ACHUPB_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0760715.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
BASE COUNT         1065 a         1115 c         1061 g         1030 t
ORIGIN      
        1 tgtgcggttt aaacaccacg gcacaaaatg acaaattcaa cgcaaaaaaa ccgggcaatt
       61 gcccggtgct ggaagggatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
      121 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
      181 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaacccgct accgacgctg
      241 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
      301 cctaaatgtt gggtcagcac cgtcaatgca gcgcctttat tgcagcccac cggcagcact
      361 tcgaggcaat ctgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
      421 tggatctgca agcgtgtaag atcgtcgtga tcgccacaga agcagatctt ggtgacgctg
      481 ccgagtggca tttttttgac atcgattatc tgataacgaa aaccgctata gacaaatgcc
      541 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa gatatgcatg
      601 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
      661 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
      721 tatgcatcca gcgatagcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
      781 gcaaaagtga gggtaatgtc gcgttcacgc agtcgcgcca aagtagagag ggttttctca
      841 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
      901 atttctttct ccactctgtg acacggttaa tggagattga gtatcacctg atatatacgg
      961 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgattg ctcaaccgtc
     1021 ttaaccagta tcaacgtctg tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca
     1081 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
     1141 cacaggaggc gggatggctg gagtggcagg agcagtcagg acgcggaaag cgcggacaat
     1201 tacgctttct ggtcacgccg gaatcgctac gcaatgcgat gatggaacag gcactggaaa
     1261 ccggaaagca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
     1321 tgttacagcc gtttatgggc ggacaatggc aaaacgatac acccacgttg cgtattccct
     1381 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
     1441 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
     1501 gcgatttagc gcatcacagg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
     1561 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
     1621 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
     1681 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
     1741 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
     1801 gtccttttcg attaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
     1861 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
     1921 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
     1981 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
     2041 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
     2101 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
     2161 tactgccagg ctggactatt ccgcactggc aagtaccgga tgaagtcaaa ctaccgaaaa
     2221 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
     2281 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
     2341 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
     2401 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
     2461 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
     2521 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
     2581 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
     2641 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
     2701 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
     2761 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
     2821 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
     2881 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
     2941 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
     3001 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtccctggg
     3061 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
     3121 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
     3181 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
     3241 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
     3301 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
     3361 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
     3421 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
     3481 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
     3541 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
     3601 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
     3661 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
     3721 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
     3781 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
     3841 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
     3901 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
     3961 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
     4021 cgccagatta cgttccacca gcgaattgcc catccccggc acctgcttta gatcctccac
     4081 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
     4141 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
     4201 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
     4261 cgctttggtt t
//
LOCUS       DAZOZD010000301         4235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOZD010000301 DAZOZD010000000
VERSION     DAZOZD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.304"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..399
                     /locus_tag="ACHUPB_004050"
     CDS             <1..399
                     /locus_tag="ACHUPB_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0760716.1"
                     /translation="HGRVVRVLDGDTIEVMDSRKAVRIRLVNIDAPEKKQDYGRWSTD
                     MMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVNQFMVRAGAAWVYEQYNTDPVL
                     PVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            445..654
                     /gene="hha"
                     /locus_tag="ACHUPB_004051"
     CDS             445..654
                     /gene="hha"
                     /locus_tag="ACHUPB_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR0760717.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            692..1282
                     /locus_tag="ACHUPB_004052"
     CDS             692..1282
                     /locus_tag="ACHUPB_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0760718.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1437..1910
                     /locus_tag="ACHUPB_004053"
     CDS             1437..1910
                     /locus_tag="ACHUPB_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760719.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            2157..2306
                     /locus_tag="ACHUPB_004054"
     CDS             2157..2306
                     /locus_tag="ACHUPB_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0760720.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            2591..2839
                     /locus_tag="ACHUPB_004055"
     CDS             2591..2839
                     /locus_tag="ACHUPB_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0760721.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            3084..3158
                     /gene="tap"
                     /locus_tag="ACHUPB_004056"
     CDS             3084..3158
                     /gene="tap"
                     /locus_tag="ACHUPB_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0760722.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            3151..4008
                     /gene="repA"
                     /locus_tag="ACHUPB_004057"
     CDS             3151..4008
                     /gene="repA"
                     /locus_tag="ACHUPB_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0760723.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         1150 a          934 c         1114 g         1037 t
ORIGIN      
        1 catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcacggaag
       61 gcagtgcgga tcaggctggt aaatattgat gcaccggaaa agaaacagga ttatggccgg
      121 tggtcaacgg acatgatgaa atcactggtt gcaggaaaaa cggttaccgt cacatatttt
      181 cagcgagatc gttatggtcg tattctcggg caggtttatg caccagacgg aatgaatgtt
      241 aatcagttta tggtccgggc cggggctgcc tgggtatatg agcagtacaa cacagatccc
      301 gtgctgcctg tccttcagaa cgaagccaga caacaaaaac gtgggctctg gtcagatgct
      361 gatccggttc ccccctggat atggagacac cgtaaataaa gcgatggcaa tcttatcatt
      421 catctgttct cagcgggaat aaaaatggcg aaaacaaaac aggaatggct gtatcagtta
      481 cggcgatgtt catcagtcaa tacgctggaa aaaattattc ataaaaacag agacagttta
      541 ttaaacagtg aacgtgaatc atttaattca gcagcagacc accggttagc ggaattaatc
      601 accggaaagt tatatgacag gataccaaaa gagatatgga aatatgtaag gtaataattc
      661 agaataatta attaccagga gcagataaac aatgaagctg attattttta ttctcattgt
      721 tctgattatt gcagcgttac ttatccggat tatcctcaaa agtgtaaatc agcattctcc
      781 tttgctgatg cagttgcatg cggcaggtat acggaccggt gatgcagagc gaatactgtc
      841 cggcggtgaa tactggcaac gtcagaagac gctgctgaca gaaagggaag tcagttttat
      901 gaaaggactg ttcagaattg tggatatgaa gcggtggtat ctgtgtccgc aggtacgggt
      961 cgcggatatc gtccagctga acgggaatat ccggccacga tcacgccagt ggtggcagtt
     1021 attcaggatg gtgtctcagt ggcatgttga tgtggttatc gttgaacggc gttcgttcag
     1081 tattgttgca gcagtagagc tggatgatgc cagccattta cgaccggaac gccggcgccg
     1141 ggatattctt ctggaagagg ttctgaggca ggctggtatt ccgttgctca gaagccacga
     1201 tgccagaaaa ctgctgcaga tgaccggaga atggctgaat acaacagggg ctgatcagca
     1261 gtccccggaa catcgtagct gacgccttcg cgttgctcag ttgtccaacc ccggaaacgg
     1321 gaaaaagcaa gttttccccg ctcccggcgt ttctgtaata aaaagcggga aatggtggtc
     1381 acagagtaaa ttccggaacc acctgtaccc aaactttata taataatggt gaatatatga
     1441 aatatgatga tgtatcagaa ttgatgatta agcgtgaaca caatattctt attgttggga
     1501 actctgcctc ccacattgat aagtttttta aggggtattc tataccaggt tctaaatatt
     1561 atgatttcac tcagattaac agtgattcag atgtagagag aaatgaggat gctgtctcct
     1621 ttatcaggga tgcaatgaat agtgaagagc agacaatcat ttttaattgt gctgggtggc
     1681 ctgatttagg tggtggatca gcagtatcac agtttgcaat gatggtcaga aaatttggta
     1741 aacaacttat tgtcgctgta agtgaaaagg atgccatcaa attaaaagac aactttgata
     1801 ttattgggat gttgtcatat ggtaaagaaa attttattgc catgagtcat acgaaaaacg
     1861 agttaactgg aggaaaacgt cggtaccgga ttaaaaatgg atgtaaataa aaagcatact
     1921 tattggctgg agtgacgcct tcgtgcttct gtagtaaaaa gcgggcaatg caggtaatag
     1981 tcagcaacag ggaatgtggt attatcgcgg cgggtgtctg agcctttctg gttcaggcaa
     2041 gacgcaggta ccagaaatgc aaagacccca cttgttaatc cattaactcg tgaggtctgc
     2101 atgaagtaca ttgcactact gattgtagcc tcttccttgc agagaggtca aagtttatga
     2161 cgaagtatgc ccttatcggg ttgctcgccg tgtgcgccac ggtgttgtgt ttttcactga
     2221 tattcaggga acggttatgt gagctgaata ttcacagggg aaatacagtg gtgcaggtaa
     2281 ctctggccta cgaagcacgg aagtaaactg ccgggcgggg acggaagtcc ccgctttccg
     2341 gaagtgtgag gtatttcagg ggcagacacc cgacatgcca gaaacagccg gtcccgcccg
     2401 gggccggctc ccgggttaag gcatttcctg ttttttcagc catttcgcaa caaaaatcac
     2461 attaaacaac agtaatcccc gttgatttgt gcgccaacac agatcttcgt cacaattctc
     2521 aagtcgctga tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagtattg
     2581 aggaggcgag atgtcgcaga cagaaaatgc agtgacttcc tcatcaggta caaagcgagc
     2641 atacagaaaa ggtaatcctc tgacgcttgc agaacgtcag caggcatccc tggcaagaaa
     2701 acgagctaca cataaagaac tcagagtgtt cattcctgct gcgttaaaag cgcaattgca
     2761 ggtgatgtgt gaagctgagg gcgttaccca ggctgagatg attgccgaac tgataaagca
     2821 gaagagtgct tttagctaac taaaatgtag cgacatcaca ttcttgcctg tttctggtgt
     2881 gcgtgataga ttgctgatcg tttaaggaat tttgtggctg gccacgccgt aaggtggcag
     2941 ggaactggtt ctgatgtgga tttacaggag ccagaaaagt gaaaaccccg ataatcttcg
     3001 acaagtttgg cgactgagaa gattaccggg gccatctaaa aaccgtatag ccaacaattc
     3061 agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcac
     3121 tccttctgcg cattgtaagt gcaggatggt gtgactgatc ttcaacaaac gtattaccgc
     3181 caggtaaaga acccgaatcc ggtgtttaca ccccgtaaag gtgccggaac gctgaagttc
     3241 tgcgaaaaac tgatggaaaa ggcggtgggc ttcacttccc gttttgattt cgccattcat
     3301 gtggcgcatg cccgttcgcg tggtctgcgt cgacgcatgc caccggtgct gcgtcgacgg
     3361 gctattgatg cgctcctgca ggggctgtgt ttccactatg acccgctggc caaccgcgtc
     3421 cagtgctcca tcaccacgct ggccattgag tgcggactgg cgacggagtc tgctgccgga
     3481 aaactctcca tcacccgtgc cacccgggcc ctgacgttcc tgtcagagct gggactgatt
     3541 acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga tatcacgttc
     3601 acatctgcac tgtttgctgc cctcgatgta tcagaggagg cagcggccgc cgcgcgccgc
     3661 agccgtgtgg tatgggaaaa caaacaacgc aaaaagcagg ggctggatac cctgggcatg
     3721 gatgaactga tagcgaaagc ctggcgtttt gttcgtgagc gttttcgcag ttatcagaca
     3781 gagcttaagt cccggggaat aaagcgtgcc cgtgcgcgtc gtgatgcgga cagggaacgt
     3841 caggatattg tcaccctggt gaaacggcag ctgacgcgcg aaatcgcgga agggcgcttc
     3901 actgccaatc gtgaggcggt aaaacgcgaa gttgagcgtc gtgtgaagga gcgcatgatt
     3961 ctgtcacgta accgtaatta cagccggctg gccacagctt ccccctgaaa gtgacctcct
     4021 ctgaataatc cggcccgcgc cggaggcatt cgcacgcctg aagcccgccg gtgcacaaaa
     4081 aaacagcgtc gcatgcaaaa aacaatctca tcatccacct tctggagcat ccgattcccc
     4141 ctgtttttaa tacaaaatac gcctcagcga cggggaattt tgcttatcca catttaactg
     4201 caagggactt ccccataagg ttacaaccgt tcatg
//
LOCUS       DAZOZD010000302         4197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOZD010000302 DAZOZD010000000
VERSION     DAZOZD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.305"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            400..1740
                     /gene="dcuB"
                     /locus_tag="ACHUPB_004058"
     CDS             400..1740
                     /gene="dcuB"
                     /locus_tag="ACHUPB_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0760724.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1818..3464
                     /gene="fumB"
                     /locus_tag="ACHUPB_004059"
     CDS             1818..3464
                     /gene="fumB"
                     /locus_tag="ACHUPB_004059"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0760725.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3587..>4197
                     /locus_tag="ACHUPB_004060"
     CDS             3587..>4197
                     /locus_tag="ACHUPB_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0760726.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
BASE COUNT         1000 a         1115 c         1074 g         1008 t
ORIGIN      
        1 taacaaaagt taattaacta ttatgtcacc cgcattatgt gtatttttac ccacaaatgg
       61 gtagatcaga ttaatctata aacctaatga catctgccct gagaacaaaa aatagaccga
      121 taaatatcaa taagataaca gcaaacaaaa cattaacatc tgcgcagtac aaactataaa
      181 cccatcgcca gagagtcttt ctctctgaaa aagccgctta tcacagtgca taaatttgcc
      241 gctgctttaa tcagccaata ttcactgtga ggtatttgct aaagccggta acgaccaaac
      301 ggatatttag tcaggctctg aaaacagttc atacaaaaca gaacgtgact gtgatctatt
      361 cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca tgttatttac tatccaactt
      421 atcataatac tgatatgtct gttttatggt gccagaaagg gtggtatcgc gctgggttta
      481 ttaggcggta tcggtctggt cattctggtc ttcgtcttcc accttcagcc aggtaaacca
      541 ccggttgatg tcatgctggt tatcattgcg gtggtggcgg catcggcgac cttgcaagct
      601 tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc tgctgcgccg caacccgaaa
      661 tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacgctga ccattctttg cggtacgggt
      721 catgtggttt acaccattct gccgatcatc tacgacgtcg ccattaagaa caacatccgt
      781 ccggaacgtc cgatggcggc aagttctatc ggtgcacaga tggggatcat cgccagtccg
      841 gtgtcggttg cggtcgtgtc tctggttgca atgctgggta atgtcacctt tgatggtcgc
      901 catcttgagt tcctcgacct gctggcaatc accattccat cgacgttaat cggtatcctg
      961 gcgatcggta tcttcagctg gttccgtggt aaagatctgg ataaagacga agagttccag
     1021 aaattcatct ccgtaccgga aaaccgtgag tatgtttacg gtgataccgc gacgctgctg
     1081 gacaaaaaac tgccgaaaag caactggctg gcaatgtgga ttttcctcgg ggcaatcgct
     1141 gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat ccttcggcgg caaaccgctg
     1201 tcgatggtac tggttattca gatgtttatg ctgctgaccg gggcgctgat tattatcctg
     1261 accaaaacca atcccgcgtc tatctcaaaa aacgaagtct tccgttccgg tatgatcgcc
     1321 atcgtggcgg tgtacggtat cgcatggatg gcagaaacca tgttcggtgc gcatatgtct
     1381 gaaattcagg gcgtactggg tgaaatggtg aaagagtatc cgtgggccta tgccattgtt
     1441 ctgctgctgg tttccaagtt tgtaaactct caggctgcgg cgctggcggc gattgttccg
     1501 gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg cttcagcacc ggcttgctac
     1561 ggttattaca tcctgccgac ttatccgagc gatctggcag cgattcagtt tgaccgttcc
     1621 ggcaccaccc acatcggtcg cttcgtcatc aaccacagct ttattctgcc ggggttgatt
     1681 ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg cgatgtacgg gttcttataa
     1741 atgcactttg cgtgccgccc gtgactacgc ggcacgccat tttcgaataa caaatacaga
     1801 gttacaggct ggaagctatg tcaaacaaac cctttatcta ccaggcacct ttcccgatgg
     1861 ggaaagacaa taccgaatac tatctactca cttccgatta cgttagcgtt gccgacttcg
     1921 acggcgaaac catcctgaaa gtggaaccag aagccctgac cctgctggcg cagcaagcct
     1981 ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa acaggttgcg gctattcttc
     2041 acgatccaga agccagcgaa aacgacaagt acgtggcact gcaattctta agaaactccg
     2101 aaatcgccgc caaaggcgtg ctgccgacct gccaggatac cggcaccgcg atcatcgtcg
     2161 gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga agaagcgctg tcgaaaggcg
     2221 tctataacac ctatatcgaa gataacctgc gctactcgca aaacgcggcg ctggacatgt
     2281 acaaagaggt caacaccggc actaacctgc ctgcgcaaat cgacctgtac gcggtagatg
     2341 gcgatgagta caaattcctt tgcgttgcga aaggcggcgg ctctgccaac aaaacgtatc
     2401 tctaccagga aaccaaagcc ctgctgactc ccggcaaact gaaaaacttc ctcgtcgaga
     2461 aaatgcgtac cctcggtact gcagcctgcc cgccgtacca tatcgcgttt gtgattggcg
     2521 gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc aagcgctcac tattacgatg
     2581 aactgccgac ggaagggaac gaacatggtc aggcgttccg cgatgtccag ctggaacagg
     2641 aactgctgga agaggcccag aaactcggtc ttggcgcgca gtttggcggt aaatacttcg
     2701 cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc atcctgcccg gtaggcatgg
     2761 gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat caaccgcgaa ggtatctgga
     2821 tcgaaaaact ggaacacaat ccaggtcagt acattccaca agaactgcgt caggccggtg
     2881 aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa agagatcctc gcccagcttt
     2941 cgcaatatcc ggtttccact cgtttgtcgc tcactggcac cattattgtg ggtcgagata
     3001 ttgcacacgc caagctgaaa gagctgattg acgccggtaa agaacttccg cagtacatca
     3061 aagatcaccc gatctactac gcgggtccgg cgaaaactcc tgccggttat ccatcaggtt
     3121 cacttggccc aaccaccgca ggtcgtatgg actcctatgt ggatctgctg caatcccacg
     3181 gcggcagcat gatcatgctg gcgaaaggca accgcagcca gcaggttacc gacgcgtgtc
     3241 ataaacacgg cggcttctac ctcggtagca tcggcggtcc ggcggcggta ctggcgcagc
     3301 agagcatcaa gcatctggaa tgcgtcgctt atccggagct gggtatggaa gctatctgga
     3361 aaatcgaagt agaagatttc ccggcgttta tcctggtcga tgacaaaggt aacgacttct
     3421 tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa gtaacctctt cggcccagcg
     3481 cctggcagca tgctgccagg tgatccccct gggccacctc ttttgcgatt gtaatttcat
     3541 gcttgctggt gaatagtcag tattttccct gacttgcgaa ctcaccatga cccgcacact
     3601 caaaccgtta attcttaaca ccggcgcact ggcgctaacg ttaatcctga tttataccgg
     3661 catttcggcc catgacaaac tcacctggct gatggaagtg acaccggtga ttattgtcgt
     3721 gccgctactg cttgccaccg ctaaacgtta tccgttaacg ccgctgctct atacgctcat
     3781 tttctttcac gccatcatcc tgatggtcgg cgggcaatac acctacgcga aagtccccat
     3841 cggttttgag gtgcaggaat ggctggggtt gagccgtaat ccgtatgaca agctggggca
     3901 ttttttccag gggctggtgc ctgcactggt ggcacgagaa attctcgtgc gcgggatgta
     3961 cgtgcgcgga cgtaaaatgg tggcgttcct agtctgctgc gtggcgctgg cgataagcgc
     4021 gatgtatgaa ttaatcgagt ggtgggcggc gctggcaatg ggccagggag cggatgattt
     4081 tctcggcacc cagggcgacc agtgggacac gcaatccgat atgttctgcg cgctgcttgg
     4141 cgcattaacg acggtgatat tcctcgctcg ctttcattgc cgccagctac ggcgctt
//
LOCUS       DAZOZD010000303         4192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOZD010000303 DAZOZD010000000
VERSION     DAZOZD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.306"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUPB_004061"
     CDS             complement(<1..215)
                     /locus_tag="ACHUPB_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0760727.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUPB_004062"
     tRNA            complement(330..405)
                     /locus_tag="ACHUPB_004062"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUPB_004063"
     tRNA            complement(412..486)
                     /locus_tag="ACHUPB_004063"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUPB_004064"
     tRNA            complement(603..687)
                     /locus_tag="ACHUPB_004064"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUPB_004065"
     tRNA            complement(696..771)
                     /locus_tag="ACHUPB_004065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUPB_004066"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUPB_004066"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0760728.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUPB_004067"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUPB_004067"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0760729.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUPB_004068"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUPB_004068"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0760730.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAZOZD010000304         4187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOZD010000304 DAZOZD010000000
VERSION     DAZOZD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.307"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..325
                     /locus_tag="ACHUPB_004069"
     CDS             <1..325
                     /locus_tag="ACHUPB_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0760731.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            552..1817
                     /locus_tag="ACHUPB_004070"
     CDS             552..1817
                     /locus_tag="ACHUPB_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0760732.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1829..2695
                     /gene="ydiB"
                     /locus_tag="ACHUPB_004071"
     CDS             1829..2695
                     /gene="ydiB"
                     /locus_tag="ACHUPB_004071"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0760733.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2726..3484
                     /gene="aroD"
                     /locus_tag="ACHUPB_004072"
     CDS             2726..3484
                     /gene="aroD"
                     /locus_tag="ACHUPB_004072"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0760734.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3628..>4187
                     /locus_tag="ACHUPB_004073"
     CDS             3628..>4187
                     /locus_tag="ACHUPB_004073"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR0760735.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1093 a          938 c         1005 g         1151 t
ORIGIN      
        1 attttatgtg gtgataatat ttgcttttgt cattggtttt acctctgctg gaggtgttgt
       61 gcaaattggc ctgacgttaa tggctgaacg tttcccttac gctaaaggta aagctacagg
      121 gatctattac agtgcgggca gtattgcgac ctttactatt ccgttgatta cggctcatct
      181 gtcccaaaga agtattgccg atattatgtg gttcgatacc gccatcgctg ccatcggttt
      241 tttactggca ctgtttatcg gcttacgcag ccgcaaaaaa acgcggcatc actcgctaaa
      301 ggaaaatgtc gctccgggtg ggtaatgcaa tattcttttc aggtcatgca agatcttacg
      361 gataaataac tctttctgcg ctaactaagg aaaatcgcga tcaaaaacaa actatgacat
      421 gcaatattcc tggaaacata aactttatgc catgtaccca gggaaaatca tcttcagtat
      481 agtaattatg taaaccgtcg gagaacaata cgtacggtaa cgaaattatc tttcagcaag
      541 gagctgtgaa aatgtctcaa aataaggctt tcagcacgcc atttatcctg gctgttcttt
      601 gtatttactt cagctatttc ctgcacggca ttagtgttat tacgcttgcc caaaatatga
      661 catctctggc agaaaagttt tccactgaca acgcgggcat tgcctattta atttccggta
      721 tcggtttggg gcgattgatc agtattttat tcttcggtgt gatctccgat aagtttggtc
      781 gtcgggcggt gatattaatg gcagtaataa tgtatctgct attcttcttt ggtattcccg
      841 cttgcccgaa tttaactctc gcctacggtc tggcagtgtg cgtaggtatc gctaactcag
      901 cgctggatac gggtggctac cccgcgctca tggaatgctt tccgaaagcc tctggttcgg
      961 cggtcatact ggttaaagcg atggtgtcat ttgggcaaat gttctaccca atgctggtga
     1021 gctatatgct gctcaataat atctggtacg gctatgggct gattattccg ggtattctgt
     1081 ttgtactgat cacgctgatg ctgttgaaaa gcaaattccc cagccagttg gtggacgcca
     1141 gcgtagctaa tgaattaccg caaatgaaca gcaaaccgtt agtctggctg gaaggtgttt
     1201 catcggtact gttcggtgta gccgcattct cgacctttta tgtgattgtg gtgtggatgc
     1261 ccaaatatgc gatggctttt gctggtatgt cagaagctga ggcattaaaa accatctctt
     1321 attacagtat gggctcgttg gtctgtgtct ttatttttgc cgcactactg aaaaaaatgg
     1381 tccggcccat ctgggctaat gtatttaact ctgcgctggc aacaataaca gctgccatta
     1441 tctacctgta cccttctcca ctggtgtgta atgccggcgc ctttgttatc ggtttctcag
     1501 cagctggcgg cattttacag ctcggtgttt cggtcatgtc agagtttttc cccaaaagca
     1561 aagccaaagt caccagtatt tatatgatga tgggtggact ggctaacttt gttattccgc
     1621 tgattaccgg ttatctgtcg aacatcggcc tgcaatatat cattgttctc gattttactt
     1681 tcgcgctgct ggccctgatt accgcaatta ttgtttttag ccgctattac cgcgttttta
     1741 ttattcctga gaatgatgtg cggtttggcg aacgcaaatt tagcacccgg ttaaacacaa
     1801 ttaagcatag aggttaaagg agttaattat ggatgttacc gcaaaatacg aattgattgg
     1861 gttgatggct tatcctatcc gccacagttt atcgcctgaa atgcagaata aagccttaga
     1921 aaaagcggga ttgccattta cctatatggc cttcgaagtg gataacgata gctttcccgg
     1981 agcaattgaa ggattaaaag ccctcaaaat gcgcggaact ggtgtgtcga tgccgaacaa
     2041 acaactggcg tgtgaatatg ttgatgaatt aacgccagca gccaaactgg tgggtgccat
     2101 caacaccatc gttaatgatg atggctatct gcgtggctat aacaccgacg gcacgggcca
     2161 tattcgcgcc attaaagaga gcggttttga tatcaaaggc aaaacgatgg tgctgttagg
     2221 ggccggaggt gcctcaacag cgattggcgc gcagggggca attgaaggtt taaaagaaat
     2281 taaactcttt aaccgtcggg atgagttctt cgataaagcc ctcgccttcg cgcagcgggt
     2341 taacgaaaac accgattgtg tcgtcacggt taccgatctc gccgatcagc aagcctttgc
     2401 tgaagtcctg gcttccgccg acattttaac caatggcaca aaagtaggta tgaaccccct
     2461 tgagaatgaa tcattggtta atgatatcag tctgttacat ccgggactgc tggtcactga
     2521 atgcgtgtat aacccgcata tgacgaagtt attgcagcag gcacaacaag caggttgcaa
     2581 aacgattgat ggatacggca tgttgttgtg gcaaggggct gaacagttca cgttatggac
     2641 tggcaaagat ttccctctgg aatatgttaa acaggtcatg gggttcggtg cctgacaggc
     2701 tgaccgcgtg cagaaagggt aaaaaatgaa aaccgtaact gtaaaagatc tcgtcattgg
     2761 tgcgggcgca cctaaaatca tcgtctcgct gatggcgaaa gatatcgccc gcgtgaaatc
     2821 cgaagctctc gcctatcgtg aagcggactt tgatattctg gaatggcgtg tggaccactt
     2881 tgccgacctc tccaatgtgg agtctgtcat ggcggcggca aaaattctcc gtgaaaccat
     2941 gccagaaaaa ccgctgctgt ttaccttccg cagtgccaaa gaaggcggcg agcaggcgat
     3001 ttccaccgag gcttatattg ctctcaatcg tgcagccatc gacagcggcc tggttgatat
     3061 gatcgatctg gagttattta ccggcgatga tcaggttaaa gaaaccgtcg cctacgccca
     3121 cgcgcatgat gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc cggaagccga
     3181 agaaatcatt gcccgtctgc gcaaaatgca gtccttcgac gccgatattc ctaagattgc
     3241 gctgatgccg caaagtacca gcgatgtgct gacgttgctt accgcgaccc tggagatgca
     3301 ggagcagtat gccgatcgtc caatcatcac gatgtcgatg gcaaaaactg gcgtaatttc
     3361 tcgtctggct ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa aaaaagcctc
     3421 tgcaccaggg caaatctcgg taaatgattt acgcacggta ttaactattt tacaccaggc
     3481 ataagcaata atatttcggc gggaacaccc tccccgccga acttaaaaaa tatatttaac
     3541 cgtatttaat aaaaatattt cgtgagtctc tgtgcgctaa ttctccatct ggcgtaggga
     3601 aaatcacatc tgaatcagga attaacaatg aaacctgtaa aaccacctcg tattaatgga
     3661 cgagtgccgg tcctgtcggc acaggaagcg gtgaattata ttcccgacga agcaacactt
     3721 tgtgtgttag gcgctggcgg cggtattctg gaagccacca cgttaattac agctcttgct
     3781 gataaatata aacagactca aacgccacgt aatttatcga ttattagtcc gacagggctt
     3841 ggcgatcgcg ccgaccgtgg tattagtcct ctggcgcaag aaggtctggt gaaatgggca
     3901 ttatgtggtc actggggaca atcgccgcgt atttctgaac tcgcagaaca aaataaaatt
     3961 attgcttata actatccaca aggtgtactt acacaaacct tacgcgccgc cgcagcccac
     4021 cagcctggta ttattagtga tattggcatc gggacatttg tcgatccacg ccagcaaggc
     4081 ggcaaactga atgaagtcac gaaagaagac ctgattaaac tggtcgagtt tgataacaaa
     4141 gaatatctct attacaaagc gattgcgcca gatatcgcct tcattcg
//
LOCUS       DAZOZD010000305         4132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOZD010000305 DAZOZD010000000
VERSION     DAZOZD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.308"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            117..530
                     /locus_tag="ACHUPB_004074"
     CDS             117..530
                     /locus_tag="ACHUPB_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0760736.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(575..892)
                     /gene="hspQ"
                     /locus_tag="ACHUPB_004075"
     CDS             complement(575..892)
                     /gene="hspQ"
                     /locus_tag="ACHUPB_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0760737.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(950..2140)
                     /gene="rlmI"
                     /locus_tag="ACHUPB_004076"
                     /pseudo
     CDS             complement(950..2140)
                     /gene="rlmI"
                     /locus_tag="ACHUPB_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2235..2513
                     /gene="yccX"
                     /locus_tag="ACHUPB_004077"
     CDS             2235..2513
                     /gene="yccX"
                     /locus_tag="ACHUPB_004077"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0760738.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2510..2839)
                     /gene="tusE"
                     /locus_tag="ACHUPB_004078"
     CDS             complement(2510..2839)
                     /gene="tusE"
                     /locus_tag="ACHUPB_004078"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0760739.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2930..3589)
                     /gene="yccA"
                     /locus_tag="ACHUPB_004079"
     CDS             complement(2930..3589)
                     /gene="yccA"
                     /locus_tag="ACHUPB_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0760740.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3796..3883)
                     /locus_tag="ACHUPB_004080"
     tRNA            complement(3796..3883)
                     /locus_tag="ACHUPB_004080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3847..3849),aa:Ser,seq:tga)
BASE COUNT         1100 a         1048 c         1017 g          967 t
ORIGIN      
        1 tcatacttac taacatatag acatatttcc cgcttttgct ctcattcatt cgtattagct
       61 gcatggttgg catgtcggct ttgtcgtaca ctttgttaaa cagtctggag gaaataatga
      121 aagaaaccga tattgcaggc attttaacgt ccactcacac cattgcgctg gtgggggcaa
      181 gcgacaaacc cgatcgtccc agctatcgcg tgatgaaata tctactcgac caggggtatc
      241 acgtcattcc ggtttcgcca aaagttgccg gcaaaacgct gctgggccag caggggtatg
      301 gcacgctggc agacgttccg gaaaaggtcg atatggtcga tgttttccgt aattcagaag
      361 cggcgtgggg cgtggcacag gaagctattg ccattggcgc gaaaaccctc tggatgcaac
      421 tgggggtgat taatgagcaa gcggcagtac tggcacggga tgccgggttg aatgtggtga
      481 tggatcgctg cccggctatt gagatccctc gcctgggcct ggccaaataa aaaatccccg
      541 gaaggcaaaa accttccggg gattcgttca gggatcagtt acgcagacgc ggggcctgga
      601 gttgtttgcg gatggtctgc gccagctcat ccatggatgg ctgttcagga tgttcatcct
      661 gcaattcgct acttagctgg gcttcagcca ggtaggtatg aaccggtagg ccgttatcgt
      721 cctccatcac tacgtgatac cacggagcgg cgcgaagctc gtcatttacc gccagctcat
      781 caggcgacgg ttcagagagc gaataaaccg ggtcgatatc cacgaccact ccgagataac
      841 ctaacaggga atggcggacc tgctggccga taccgaattt gctggcaatc atagtcacct
      901 cccgggaaat ctttaccttt gatataaggg taaaattcca catttcaagt tacatgacgc
      961 gacaggcaaa ccctttcaga tatagccctt ccggataggt agcgatcacc ggatgatcgg
     1021 ctgcctgacg gaactgctct ataaattgta catcacggcc ggcatcaatt gcggcatccg
     1081 cgatgatttt ctgaaataaa tcgctggtca tcagaccgga acaggagaaa gtcaggagaa
     1141 taccgccttc attcagcaac tgaatcgcca gcatgttgat atctttatag ccacgacacg
     1201 cgcccatcaa ctggctttta ttctcaacaa acttcggcgg gtccatcacg ataacgtcaa
     1261 atttttcacc gcgatcgcga taagtacgca gcaatttaaa gacatcatca cggacaaact
     1321 cagccttgct cagatccagt ttgttcagct caacgttctg ccgtgcaata tccagcgctt
     1381 cctaggaggt atcaacgctg acaacctggc tgcaaccgcc catcagtgcc gatacggcga
     1441 aaccaccggt ataggagaaa cagttcagca cacgtttatt ttcaacgtag cggcgggtag
     1501 ccaggcggct gtctcgctgg tccaggtagt agcccgtttt gtgtccgtgc tgaatatcca
     1561 ccagcagctt catgccgtgt tcttcaatcg gcagcagggc aggcggcaac tcgccggtga
     1621 cgaggccctg ggtcagctcc atcccttctt ttttacgtac cgcaacatcg ctgcgatcgt
     1681 aaatcgcaca ttccgggtac agcgtttgca gggcactaat taatgccgcg cgctgatatt
     1741 ctgccccagc actcagcagt tgcagcacca gaaaattacc gaaacgatcg atagtaatac
     1801 ccggcaggcc atcagattct ccggcgatta aacgatagct gtcgaggcca tctttttgcg
     1861 ccagtcagtc acgccatttt tgtgcttgtt gcaaacggcg ggaaaaaaaa gcaatgtcga
     1921 tagactcaga cgggtcaaac gtccagacgc gcgcccggat ttgcgaagct ggcgaataag
     1981 cgccgcgtgc taaccatttt ccctgatgat caacaatatc gatggtttca ccgaggctgg
     2041 ctttaccttc catgcgggca acggccccgg aaaagaccca cggatggcga cgaagtaatg
     2101 atttttcgcg ccctttggct aacactaaac gtacactcat aatttacttt tctgtcgatg
     2161 caaagaaatg ggcgtcattg tccggagttt tccatggatt tgcaacgcgt ccattaagga
     2221 taaggagagc agcaatgtcg aaagtctgca taattgcctg ggtttacggg cgagttcagg
     2281 gcgtaggatt tcgctacacc acacagtacg aagcgaaaag actgggttta accgggtacg
     2341 ccaaaaatct tgacgatggc agcgtggaag tggttgcctg cggtgaagaa gggcaggtgg
     2401 aaaaattaat gcagtggtta aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc
     2461 tcagcgagcc gcatcatccc tcgggggaat taaccgattt tcgtatccgt taaatacatt
     2521 ttaccggttt tggcaggcca gcaattttgg tggcttgctt agccggacct ttcggaaaca
     2581 gtcggtataa atagcggctg ttaccttttt cttcgccaaa tttattcgcc atcgctttta
     2641 ccagcatacg aatcgccgga gaagtattga attccagata gaaatcacgc acaaaacgca
     2701 ccacttccca gtgttccggt gacagcgaaa tcccttcgtt ctctgcaatc accactgcca
     2761 gcggctcact ccactggctg ctttctttga gatagccttc ggtatccgtt tctatctctt
     2821 taccttcgaa gatcagcata attattacta ccttaatcag actggcggca gtgtaacaaa
     2881 aaacaaagcc ccgcataaag cgaggctatg aaagtgttag cgggtgagat taatcgcggc
     2941 tggcgaagcc cagaatgctc agcaggctga cgaagatgtt gtacagcgaa acatacaggc
     3001 taaccgtggc acgaatatag ttcgtttcac cgccatgaac gatgttgctg gtttcaaaca
     3061 aaatagcgcc agaggagatc agaatgaaga ccgcgctgat cgccagatgc agagcaggca
     3121 gctgcaggaa gatattagca accataccaa tcagcaccac cacaataccc gccatcagca
     3181 taccgccgag gaacgacata tctttgcggg tggtcagcac atatgcagag cagcagaaga
     3241 acactaacgc cgttccgccc agtgccatag cgattacgtc acccattccg gcagacagat
     3301 aggtgttcag aataggtccg aggatataac ccagaaaacc ggtaaaggcg aatgcggaga
     3361 taatcccggt cggcttattc gccgttttat aggtcaggaa catcaaacca tacataccca
     3421 ccagcgtcag aatcagaccc ggagatggca gcatcagcac cgtactggca gttgcggtaa
     3481 tcgccgaaaa ggccagcgtc aggctcagca gaaaataggt attacgcagc accttatggg
     3541 tgctaagcag tgatgtacgg tcatgtgaag aactaacaat acgatccatt gagtcactct
     3601 ctatgacaga tgtaattaat taagcagcat aatgataatg cgtaagggca cccagaagtt
     3661 ttacccatct ttacgcattt gatctggaac aggtttaaca gcggattatc aggtcattaa
     3721 gcaaatataa cgccctgaga atttcgacag gcaaaagaaa aaggggttag catttagcta
     3781 accccttatc ttatttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
     3841 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
     3901 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
     3961 agtgcattga tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct
     4021 cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggcaaaaag aaaaataggt
     4081 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aa
//
LOCUS       DAZOZD010000306         4130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZOZD010000306 DAZOZD010000000
VERSION     DAZOZD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.309"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(183..1502)
                     /locus_tag="ACHUPB_004081"
     CDS             complement(183..1502)
                     /locus_tag="ACHUPB_004081"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0760741.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1633..3168)
                     /gene="gadC"
                     /locus_tag="ACHUPB_004082"
     CDS             complement(1633..3168)
                     /gene="gadC"
                     /locus_tag="ACHUPB_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0760742.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3324..>4130)
                     /locus_tag="ACHUPB_004083"
     CDS             complement(3324..>4130)
                     /locus_tag="ACHUPB_004083"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0760743.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1060 a         1028 c         1051 g          991 t
ORIGIN      
        1 cgaatatcgt atggtttttc atattcatac atttttatta gggatttata gctgtttaac
       61 taagtgtggt taattgcgtg gttaatttga cttatgtaag catgattact agtgggatat
      121 ttcacaaggt gtaacaagcc ggtgggtaaa gcaccggctt gttacaaagt aagcatggga
      181 gtttaactgc cccaacgact ttgcagatag ctgaccgctt gttgagtctg cggtttattc
      241 agatagtcct cacggaacaa gatggtgccg ctaatttctg gtaccgcatc gttaagatcg
      301 agctgctttt tcagttccgg tacgccgccg ttaatcatcc agtctggctc tatctttgaa
      361 ggttcaccca ctttatagaa ggcgataccg atatacaggc gggtcctagt cggtttaacg
      421 acatccgccc accattttgc caacacgtca taacgcgcgg cactccgtga gaacggccag
      481 taaatttggg gagcaatgta atccagcaat ccttgttcca cccatcgacg ggtgtcagcg
      541 taggattcgt cataggctgc cgcgcctcgg gtatcggaac cgagcggatc gtgtgatcgg
      601 ttacgccaca cgcctgccgg gctaacgcca aattcgactc ccggcttaat gcttttaatg
      661 gtgtgcgata cttttgcaat taactgctga gtattgttgc gccgccagtc tgcttttgac
      721 gcaaatgcgc ctccgtattt gcggtacgtt tcgttatcat ttagccgtga acccggtgac
      781 tcagtataga aatagtcgtc aaactgcacg ccatctaccg gatagcggga aaccacttct
      841 gcgactattg atgtgatcca gtcctgaacc tcagggatgc ccgggtcgag gacaaagcga
      901 tcgccagacg ttctgatcca gtcacggtgt tgcacataga cgctcgccgg ttgttgagac
      961 agagtgctat tcagttccct gatagtaccg ggcttcgtat taaccgatac gcgatagggg
     1021 ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt cgtcgagcat aaattgcagc
     1081 ggatcgtaac ccggattttc accaatctta ccggtcataa gatcggacca tggcaaaatt
     1141 ttcgatggcc acagggcggt accgtccggc ttaacctgga aaaagaccgt gtttataccg
     1201 agacgttgca gatgatccag tttgtcgatc atcgcctgtt gttgtacacg ggcccggctg
     1261 gtggggttac taatgttaac cgaggaaacc ggtggccagt cgaggcgaga caccgtggcc
     1321 agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg tggctggtgg ctttgaacct
     1381 gctggtggtg tcaccatgga ctctggaggc gtgcttttac aactacacag taaaagtgcc
     1441 agtgcaacca gtatcgctgg tcttctaatc gctaatttct cgtttcggga gcagatatcc
     1501 atatatgctg gtttccggta aacagatgtg cgctcattct cgtacttatc ccggttaagt
     1561 caatacgaca gcgagcacga aaaagggagc gatgaattat cgctcccttg tcttataacc
     1621 attcagacat ggttagtgtt tcttgtcatt catcacaata tagtgtggtg aacgtgcacg
     1681 cgggtgcagg aagaagtgac cttttggtgc gttctgactg ttgattggct ccagagtgac
     1741 gccggtattc gctttgcctt tacgatcatg aacagcatag agaataaagg gcagggcaag
     1801 taccaccagg aaactaacga ccagtaattc aacatacata tcggtagagt caccctggat
     1861 gttatccggc ggcaggaagg aaacaataaa cgccataatt gaagtcagca gaccgacaat
     1921 agccacgacc agtttcaccc ctttaccacc agggatatta aatgtgcgtt ttaagtcagg
     1981 atgtttaaga accaacacaa tgtagccaat aaacagcatg aaataagcac acagataaat
     2041 caccaccgtc agcgccagtg cgatcaggaa ggacatgttg ttaccgccac cggtattggt
     2101 gaggatgatc aacgcgatag aagtaatcac cagctgtgaa atgaccagcg ttaccggtac
     2161 gccatttttg ttcattttag cgaatgccgc tggcagcagg tttttctgcg ctgtgacata
     2221 catcccgcga gaaggaccaa caatccagga ggcgatttcc gccagaacac ccagcaacag
     2281 cagtgcggag atcacgcgaa ccgtccactc aatttccggt gccacatggg acatcagaac
     2341 ggtaaaggtt tgcattaccc ctgcggagag gttgatttca ttacccggaa tgaccatcgc
     2401 aatagacaaa ccaccaacag agcttaagca gattgccgct accatcagca gtaacatagc
     2461 caacggatag tcgcgccctg ggttgctcat ttcattgacg tgggttgcgg atgcttctac
     2521 gcccatataa ctcaaaatga aggcaacaaa tacaaccagg gtgcccactt tagagaagtc
     2581 agggaagaag gtctttgaat ccatttcgat agcaacgggg gcaccggagt gcagataaat
     2641 agccgctaat gcgatcaaaa taaatgcagg taacaggata ccggcgaaga agccaacttt
     2701 agcaattcgt gccgtgtatt tcgtgccacc aaactgcgtt aatgccagcg cccaaagaat
     2761 gatgagtgct gcaatagttt tggtaatggg gtcttcattc agcgctggcc atttcaggat
     2821 gtaggagagt gcccctaaca cgaaatagag catcggaata aaaccaatgg cgatttgcag
     2881 atagccaaat gagatcgctg caaatcccca tctcggcccc agagtatttg atacccaggc
     2941 gaagacacca ccttcttccc agccgtcgac ggtggccatt tccgcagcac aaagtcccac
     3001 gggaataaac cataaaatcc cgcctaatag caggaagaag actaatgaaa agcccgatgt
     3061 tgcgaaggta gggtattcat aaacagccat taccatcgat gccgttatgg caaaaaagcc
     3121 gagtaatgtg agctgcttag ctttacctgt ctgtactgat gtagccatat tatcccccta
     3181 aaacggtatt cctgtcggaa ccgcacctgt tttgttctga agcgtattca gaacaatatt
     3241 ttccgttgct aatgccagtg aacagacttt ggaaattatc ccgaaacggg ttcgtttcgg
     3301 gacaccgtta ccgttaaacg ttatcaggta tgtttaaagc tgttctgctg ggcaataccc
     3361 tgcagtttcg ggtgatcgct gagatatttc agggaggctt tgtagtcttc cagcaacagt
     3421 tcagcaaagt ccatttcgaa gccgcgacga cacataatgc gcatcaccac gatgtcggtg
     3481 gcttcaccgc cgagagtgaa ggccggaacc tgccagccgc gcagacgcag acgttcagag
     3541 aggtcgtaca gggtgtatcc cggatcttca ccctctttca gtttgaagca aaccgccggg
     3601 atgccttcgt ccgggcgacc ggcacagatg aactcatacg gccccagttt ggcgatctca
     3661 tccgccagat aggcagcgac ctggtaggaa gcattctgta ctttggtata gccttcacga
     3721 ccaaggcgca ggaattcata gtactgtgca atcacctgac ccgccgggcg ggagaagttg
     3781 atggcaaaag taccaatctg accaccgagg tagtcaacgt tgaacaccag ttcctgcggc
     3841 agcgcttctt cgtcacgcca gataacccag ccgcagccca gcggagccag accgaatttg
     3901 tggcctgaag cactgatcga tttcacacgc ggcaggcgga agtcccagac gatatccggg
     3961 gcgacgaacg gtgccaggaa gccaccgctg gcggcgtcga tgtgcatgtc gatgtcgata
     4021 ccggtgtcgg cctggaattt atccagtgca tcgtgcagcg gttgcgggaa ctcatagtta
     4081 ccggtgtagg tcacgccgaa agtcggcacc acgccgatgg tattttcgtc
//
LOCUS       DAZOZD010000307         4124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOZD010000307 DAZOZD010000000
VERSION     DAZOZD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.310"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHUPB_004084"
     CDS             <1..83
                     /locus_tag="ACHUPB_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0760744.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="ACHUPB_004085"
     CDS             459..566
                     /locus_tag="ACHUPB_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0760745.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHUPB_004086"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHUPB_004086"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0760746.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHUPB_004087"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHUPB_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0760747.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHUPB_004088"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHUPB_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0760748.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1051 a         1029 c         1074 g          970 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccacccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAZOZD010000308         4107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOZD010000308 DAZOZD010000000
VERSION     DAZOZD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.311"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHUPB_004089"
     CDS             complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHUPB_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR0760749.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
     gene            981..1274
                     /gene="yicS"
                     /locus_tag="ACHUPB_004090"
     CDS             981..1274
                     /gene="yicS"
                     /locus_tag="ACHUPB_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR0760750.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHUPB_004091"
                     /pseudo
     CDS             complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHUPB_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2706..3158)
                     /locus_tag="ACHUPB_004092"
     CDS             complement(2706..3158)
                     /locus_tag="ACHUPB_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR0760751.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHUPB_004093"
                     /gene_synonym="yicO"
     CDS             complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHUPB_004093"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR0760752.1"
                     /translation="LESLPGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGI
                     LGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSG
                     ALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAG
                     AFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALI
                     FVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRW
                     RDLNLCVVVVASLFALKIILVD"
BASE COUNT         1211 a         1080 c          911 g          905 t
ORIGIN      
        1 tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat cttcccgtgc
       61 caccaaaata ttcacatacg gcgaattctt atcttcaata aatacgctgt cgtgcaccgg
      121 agaaagccct gtctgctgaa tgtaagtggt gctgataatc gctacatcaa ctttgggatc
      181 atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat gacgaggatt
      241 atcggttata tccagcgcgg taggtaataa cccttttccc tctttcagag taattagctt
      301 ctctttttgc aacagtaaaa gtgcgcgacc aaggttggtc gggtcgttgg ggatcgccac
      361 tgtcgcgcct tcttttattt gcgccacggt tttgattttt ttggaataac ccgccatagg
      421 gaagacaaac gtatttccca gcgctaccag tttataacca tgcgcctgat tatcttgttc
      481 aagaaagggt cgatgctgga agacattcgc atcaagttcg ccatgatttg tcgcatcgtt
      541 cggtagcaac gaaccgctaa atcctaccag ttcaacatcg aggccatatt tctcttttgc
      601 caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc caactttaat
      661 gtgttttgca tcgctgctac tctggtcgca acctgccagc agaattccgg ccagcaataa
      721 tgcggcccct gtccgtagat gatgtgttgt cagtttcatt tttttatcct tttattaatg
      781 attacctgac tatagcggta aggtcgctgc ggtttaaaaa acgaaacgct atcgataaga
      841 ataaaaagga ataaaagtgg aatataaagt ctggcacttc ttactgacca cgcaagcgcg
      901 tttcgtacag catgatgaga gcgatgaaag caaactacac ttatgcttca ttcgatacac
      961 atttgtaaaa ggttgaatcc atgaaaccaa cgacactact gcttattttc acttttttcg
     1021 ctatgccagg catcgtatac gccgagtcgc cttttagctc actgcagtcc gcaaaagaga
     1081 aaaccacggt attacaagat ttacgtaaaa tttgcacgcc acaggcgtca ttatcagatg
     1141 aagcgtggga aaagttaatg ttgtcagatg aaaacaataa acaacatata cgcgaggcca
     1201 tcgtggcgat ggaacgtaat aatcagagca attattggga agcgttgggt aaggtagaat
     1261 gcccggatat gtaagaacag caacaactac tgaccaacgc ttctttacct gatatcagga
     1321 tttcttcatt ttcacctttg cagtaaccaa caatgcagtc agcaacatca atgtgccaga
     1381 aaacatcaac ggcgaagtca gaccaatatt atccagcgca taaccgccga ttgccgcgcc
     1441 acaagctgaa taaccgccac ctgaatagac ccggcttttt ctgcctgatc ggccagtgag
     1501 cgggtgatcc acgttgacca gccgacggga accaatgcaa aagttagccc ccagataatc
     1561 gccacgccgg tagcaacgat tttatcgctt ccccacaacg tcagcaccaa cgcactcaca
     1621 gccagtatta acggcgcgcc tgctaaggcc agttttaccg aacgtttaag aatgaatgac
     1681 gaaagcgacg taccgataaa gctggcaatg ccaaaactca acagcaccag cgttaagcca
     1741 tccacgctga atcccgccag gttcatatac accgggcgaa tatatgtgaa gaaagcaaac
     1801 tgcccggcga aagacatgaa gatggcgatc atccctgcca tcacacccgg acgttgtaat
     1861 aagcggaaag tattttgttt ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg
     1921 ataatccaga aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa
     1981 ccgataagct cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc
     2041 gcgccgaaga tcaccgacag cgccttcggc accgtacgcg gcggcaccag acgcatggtc
     2101 agcgacgccg acatcgccca gaacccgccc agcgccagcc ccagacaggc acgaccgatt
     2161 aaaagcaaac tgaatgagtt agcaaaggaa accagcaagc aggagagcgt cagcaaaacg
     2221 gcaaacaaaa taacaacgtt gcggcggtca gtagcctgaa ttgtctgggt aataaacaaa
     2281 ctggcaaaca ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa
     2341 atgcctaaat cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataatc
     2401 agacaggcga cacaaaacgc caccgagaaa acggctgacc agttcggtcg ggtgatggca
     2461 tccgcgccgc ggttttcggc aataaattca ctcatggtgt tacccgtggc atgtttcagg
     2521 ggaaaagccg accactttaa cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt
     2581 acggtgttgc ttgagctgga gttaagatcg aaacggaaca gggcaaagat taccggatgc
     2641 ggcgtgaacg tcttatccac ccgacaggtc aggcgcggtc tgtaggctga taagacgaag
     2701 cggttttaat gcagccagtg aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc
     2761 tgaaaagcat taatatcctg catttcaggt tcggtttcgc gcagaaaacc cagcgccagc
     2821 ctgacctgag catcggtaat cggcgcggaa aggcgggctt tttgtaaaat ccgatgccat
     2881 tgcagcaggt tctgttcgct accatcgaca atgacaattt gccagatgag caacacctgc
     2941 gcggcgtttt gcaggtgcga ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg
     3001 gctgactttc ccatttgatc gaccatcgac tccacgggta cgacatcgat attcagccga
     3061 tcactgagac ggcgaatcgc ttttctggcc ccggatgtca gcacccgaaa accaaccaca
     3121 aacactaccg ccagcgtggc gagcattatc cagatcatga gttctcctgc gttatcagaa
     3181 ctcatgatat ctggaaaccc tcgccagtta ctaatccacc agaataatct tcagtgcaaa
     3241 cagagatgcg accaccacca cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt
     3301 catgatgcag tacgacataa agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg
     3361 catcatcacc gtggtaataa acgcaggcac cgattcggta aaatcatccc agttaacgcg
     3421 cgccaggctc gaagtcatca gcacgccaac aaagattaac gctccggcgg ttgcgtaagg
     3481 aggaaccatc gccaccagcg gggagaagaa cataaccaac aggaacataa cgccaaccac
     3541 aaccgcagtc agccccgtgc ggccaccgac tgccacacca gaagtacttt caatataggc
     3601 agtaacagac gaggtgccga taaacgcacc cgccaccgaa ctgacgctat caacatacag
     3661 cgccttattc atattgggga atttaccgtt accatctatt aagcccgctt tatcagttac
     3721 accaattaat gttcctgatg aatcaaatag gttgatcagc ataaaggaga aaatgatacc
     3781 ggcgagttca agtgttaacg cgccgctcaa atctacttca ccaatgacgc cgctaatatc
     3841 aggcggaatg gaatagacgc cgctaaaatg aacatcaccg aaaaataatc cacagcaaga
     3901 cgtcaccaca atagaaacca gcaccgcggc atgaaaatga cgtgatgaca acacggttat
     3961 aataaaaaaa cctaaaatac ctaacaacac gccgtgagaa cttaaatcgc caatcatcac
     4021 cagcgtgtct ttattggcga caataacgcc agtatttttt aatcccatta aggcgataaa
     4081 taatccaatt ccgggtaatg actccaa
//
LOCUS       DAZOZD010000309         4082 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOZD010000309 DAZOZD010000000
VERSION     DAZOZD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.312"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(46..747)
                     /locus_tag="ACHUPB_004094"
     CDS             complement(46..747)
                     /locus_tag="ACHUPB_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0760753.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPK
                     LQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYAKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIIALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1280..1534)
                     /gene="mobC"
                     /locus_tag="ACHUPB_004095"
     CDS             complement(1280..1534)
                     /gene="mobC"
                     /locus_tag="ACHUPB_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR0760754.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1713..3872
                     /gene="mobQ"
                     /locus_tag="ACHUPB_004096"
     CDS             1713..3872
                     /gene="mobQ"
                     /locus_tag="ACHUPB_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR0760755.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEAL
                     PKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGL
                     AALRVQLLKREEELRRKEQEDTERSRTERRAEQLRKNDNAVMDSGVLSAVVTGYGEAP
                     MPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHP
                     PLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNS
                     QLVISALTDKGLRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQP
                     DHLFRSLGFNRTWDEWRREEDARTHATERRHDRGWSQ"
BASE COUNT         1075 a         1032 c          985 g          990 t
ORIGIN      
        1 aaaaaaaacg cagcagggag cgttttaggt gccttaaaat cgattttaga tcgtcatctt
       61 gatttgtttg tcttccttga agcgaaaatg cagggctaca accgtgcgcc cctttttgat
      121 cgtctcaacc gttacagcca ggtcgctttt tttgttcagt tcgtcaatgc agggtcttaa
      181 taattgctgg ttaagtgatt tatatgtttg atatttatgc tctatatcaa gcattgagcg
      241 aaaatcatcg agagcgataa tacgttcgcc agtactgcgg aactgctgga ggagttcgta
      301 tatccttata ctgtaacttc ttgataatct agatatattc ttgattacaa ctctagtaaa
      361 ttgcccttgt agttgtgtca aataaggcat tactgcgtca gaaaatgtta tttgtgctct
      421 ggcctcgcct ttagcgtact gtgcccgata ctggatccag cggaactcct cacgtttctc
      481 gtcatccttg agaatgattg acctgtccca caaacgatca attgcttcct gtaactgaac
      541 ctcggcattt ttccgcccca tgtcagggaa tgaatcgaaa tattccgcag ctgtaatggt
      601 cattgtcttt tgcaattttg gagattctgc atcagcccca gacttcaatc gaccgatgca
      661 aagtaacaaa aatcgctgtt cctgtagggt caatttataa ctggcttcaa ccagcgcatt
      721 ggatttatag gcaattagat ccgacataga gcatccctcc ccaaaaggta atgaataaaa
      781 gataaacata ccctcaacga aaggcaatgc ctttttattt taatatccct ttttctgtga
      841 atattacctt aaatcatatt gcctttacct taaattcccc cacctaaaca ccttaaatca
      901 tattaccttt accttaaatc acattaccta tagcattgta acattttgtt ttctataagg
      961 aattttggcg ctaaaacaag tattaaaaac aagatcttaa aaacaagatc taaaaactta
     1021 acgacgagcc tgtggataac ttggagtgct gaaaacaaaa aaccaataaa aacaatgcgt
     1081 tgcaaaaaag cagaattgca agttccgaat acaccccctc caaccctgtg gaaatccagt
     1141 attcatgcgg gttcagcgca tatcacctcc cgaaaaaccg tgcgtatgtg ctcaatcagg
     1201 cgacggattc acttccgctt cctcgcccac tcgctcgcta acgctcggtc gctgggactg
     1261 cggcgagcgg acgatacaat cactgtcctg gcagcgcttc cagctcgtta atcagcggca
     1321 gtatgcgcgt cacatcagcc tgtcgtgtca cctctgcctt caggaactgg agtaaccagg
     1381 cacgcaactg cacctcctcc ggctttctga cctttgccag caatgctgca cccacaatga
     1441 ccttctgcgc tgtgtccaga ctcctgctct tcgccttcag gcgctgtaat ctggcctcag
     1501 catcagcaat cttctgttcg agtgttctgc tcatgatgta actccgtgcg cgatgaaaaa
     1561 tcgcatttta gcgcgtcact ggtagtttaa aaactgagct ggcataatgc acggaaaatc
     1621 acgaagtgcg cacttataca atctccactc cgtttcgatt gtgtgcgccc tgcggggctg
     1681 aaagaaaacc acaaaaaggc attacggcag aaatggcgat ttatcatctc agcatgaaaa
     1741 tcatctccag aaacagcggc tacagcgccg ttgcttctgc tgcctatcgt tccggttcac
     1801 tgatgcttga tgagcgtacc ggactgactc acgactacac ccgcaaaagc ggtgtggctg
     1861 aagctgtgat tctgactccc gcgacagccc ctgcctggtg tacgaaccgt gccgaactct
     1921 ggaatgccgt tgagaaggcc gaacgccgga agaactcaca acttgcgcgg gaaattgaac
     1981 tcgccatccc ccgcgaacta ccgcaggatg ccgcacggga aaccgttctc gcctttgtca
     2041 gagaaaactt tgtcagtcag ggcatgattg ctgatgtggc gttccatcac atggataaaa
     2101 caaatcccca tgcgcacatc atgctgacca cgagagctgt cggacctgct ggattcggtg
     2161 gaaaggtcag ggactggaac gaccggacac atgccgagac gtggcgtgca tcatgggctg
     2221 accatgcgaa cagggcactg gcgaacgccg gttatcagga agagatagac catcgttcat
     2281 acgagcgaca ggggcttgag aaaacgcctg gtattcacct cggaaagtct gcctgtgcga
     2341 tggaaacacg cggtatcgaa acagaacgcg gcgaacagaa tcgtctgatt aacagactta
     2401 acctggaaat acagatttcc cgtaccgaac tcagaaacag cgggctgaca gagcctgccc
     2461 gccgtgtggc tgaccttctg aatatcgtgc ttcctgacaa cgcaacttcg tacacactac
     2521 gtgacatcat cgaagcgttg ccgaaggaca gtaatgcggc atggaaactc accagtaatg
     2581 ccatgtcgat gatggcagat atgcaggcga cgcgcagacg ctgggaagag ctgaacaccg
     2641 aacgaaagga agcgatgagc catgctgcca gcctgaaaaa atcatcgccg ttttcgtccg
     2701 gtttttcccg tattcccctg atgcaatggg cggcaccgga ataccgtagc gaacagggaa
     2761 aaatccagtc gctgagccag caaatggaaa aattgcgaca gcattaccgg aaggtggaga
     2821 aacaggacat tcccgcgagt cagacggcat ttgaaaaaca gtggcagcag tggggggcat
     2881 ccggccttgc agctctcaga gtccagttgc ttaaacgtga agaggaattg cgccggaagg
     2941 agcaggagga tacagaacgt agtcgcacgg agcgacgagc ggaacagctc aggaaaaatg
     3001 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
     3061 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
     3121 acacgctctg ggggaacgag ctggagaaat accgcaccag aatctttgag cgtgttgatt
     3181 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggtcaa catccaccac
     3241 tgcaacgtgt ttattcctct gcgacctttg agcaacttct gacgcaggtt tgccagacat
     3301 ggccgcaata tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     3361 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     3421 gacgtctgct accgacgctg atgccggtaa tgagttctgt cgaccgggaa acccgccagc
     3481 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     3541 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     3601 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     3661 acagccacat atcacctgcg ccggaactta tggagtttca tctgaaaacc cgtgaggcac
     3721 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     3781 gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca cacgccaccg
     3841 agaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtttaac
     3901 atcgtgttat gttcgtcgat gttcttcgat gttcatcgac agggatgaat gcaagaagca
     3961 cacacataat gcaagcatag ttttgttttt ccgcccccaa aaaatggggg ttcttttttc
     4021 gctgcattag agcgcttttg acgcaatttt tcacttcaat agtaccttcg cactactaac
     4081 ac
//
LOCUS       DAZOZD010000310         4067 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOZD010000310 DAZOZD010000000
VERSION     DAZOZD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.313"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(230..877)
                     /gene="ychE"
                     /locus_tag="ACHUPB_004097"
     CDS             complement(230..877)
                     /gene="ychE"
                     /locus_tag="ACHUPB_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0760756.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1355..4030
                     /gene="adhE"
                     /locus_tag="ACHUPB_004098"
                     /gene_synonym="adhC"
     CDS             1355..4030
                     /gene="adhE"
                     /locus_tag="ACHUPB_004098"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0760757.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1130 a          998 c          944 g          995 t
ORIGIN      
        1 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
       61 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      121 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      181 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      241 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      301 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      361 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      421 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      481 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatgctt tcacgtaccg
      541 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      601 acatcgctat cgtcaccacc aggatgcccc ctgcgatacg gaacgaatca attgatatac
      661 caaaaagttg cagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      721 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      781 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      841 tgaagtaaac gggaaaatca aaaaaggtct gaatcacggt tagctccgaa gcaaaagccg
      901 gataatgtta gccataaatt aaggttgaaa agacgcgctg acaatacgcc ttttgacagc
      961 atttttcacc tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca
     1021 aaacatgcta atgtagccac caaatcatac tacaatttat taactgttag ctataatggc
     1081 gaaaagcgat gctgaaaggt gtcagcttag caaaaatttg atttggatca cgtaatcagt
     1141 acccagaagt gagtaatctt gcttacgcca cctagaagtg acgcattaga gataataact
     1201 ctaatgttta aactctttta gtaaatcaca gtgagtgtga gcgcgagtaa gcttttgatt
     1261 ttcataggtt aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt
     1321 tactaaaaaa gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact
     1381 taacgcactc gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga
     1441 gcaagtagac aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact
     1501 cgcgaaaatg gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa
     1561 ccactttgct tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct
     1621 gtctgaagac gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg
     1681 tatcgttccg accactaacc cgacttcaac tgctatcttc aaatcgctga tcagtctgaa
     1741 gacccgtaac gccattatct tctccccgca cccgcgtgca aaagatgcca ccaacaaagc
     1801 ggctgatatc gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg
     1861 gatcgatcaa ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct
     1921 gatcctcgcg actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc
     1981 tatcggtgta ggcgcgggca acactccagt tgttatcgat gaaactgctg atatcaaacg
     2041 tgcagttgca tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga
     2101 acagtctgtt gttgttgttg actctgttta tgacgctgta cgtgaacgtt ttgcaaccca
     2161 cggcggctat ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa
     2221 cggtgcgctg aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg
     2281 cttctctgta ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag
     2341 cgaaccgttc gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt
     2401 cgaagacgcg gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc
     2461 ttgcctgtac actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat
     2521 gaaaacggct cgtatcctga ttaacacccc agcgtctcag ggtggtatcg gtgacctgta
     2581 taacttcaaa ctcgcacctt ccctgactct gggttgtggt tcctgggggg gtaactccat
     2641 ctctgaaaac gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga
     2701 aaacatgttg tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat
     2761 cgcgctggat gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt
     2821 cctgttcaac aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga
     2881 aactgaagtc ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc
     2941 agaactggca aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat
     3001 ggacgccgcg aagattatgt gggttatgta cgaacatccg gaaactcact tcgaagagct
     3061 ggcgctgcgc tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa
     3121 agcgaaaatg atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc
     3181 ggttgtaact gacgacgcta ctggtcagaa atatccgctg gcagactatg cgctgactcc
     3241 ggatatggcg attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt
     3301 cggtggtctg gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga
     3361 gttctctgat ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc
     3421 ctaccacgaa gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat
     3481 cgcgggtatc gcatttgcga acgccttcct gggtgtatgt cactcaatgg cgcacaaact
     3541 gggttcccag ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat
     3601 tcgctacaat gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc
     3661 gcaggctcgc cgtcgttatg ctgaaattgc cgaccacttg ggtctgagcg caccgggcga
     3721 ccgtactgct gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact
     3781 gggtattccg aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt
     3841 ggataaactg tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc
     3901 gctgatctcc gagctgaaac agatcctgct ggatacctac tacggtcgtg attatgtaga
     3961 aggtgaaact gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa
     4021 atccgcttaa tcagtagcgc tgtctggcaa tataaacggc cccttct
//
LOCUS       DAZOZD010000311         4054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOZD010000311 DAZOZD010000000
VERSION     DAZOZD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.314"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..414
                     /gene="hiuH"
                     /locus_tag="ACHUPB_004099"
     CDS             <1..414
                     /gene="hiuH"
                     /locus_tag="ACHUPB_004099"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0760758.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            523..1527
                     /gene="msrP"
                     /locus_tag="ACHUPB_004100"
     CDS             523..1527
                     /gene="msrP"
                     /locus_tag="ACHUPB_004100"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR0760759.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1528..2163
                     /gene="msrQ"
                     /locus_tag="ACHUPB_004101"
     CDS             1528..2163
                     /gene="msrQ"
                     /locus_tag="ACHUPB_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0760760.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            2420..3070
                     /gene="zinT"
                     /locus_tag="ACHUPB_004102"
     CDS             2420..3070
                     /gene="zinT"
                     /locus_tag="ACHUPB_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0760761.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            3413..3943
                     /locus_tag="ACHUPB_004103"
                     /pseudo
     CDS             3413..3943
                     /locus_tag="ACHUPB_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT         1141 a          928 c          880 g         1105 t
ORIGIN      
        1 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
       61 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
      121 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
      181 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
      241 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
      301 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
      361 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
      421 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
      481 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
      541 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
      601 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
      661 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
      721 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
      781 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
      841 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
      901 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
      961 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     1021 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     1081 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     1141 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     1201 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     1261 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     1321 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     1381 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     1441 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     1501 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     1561 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     1621 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     1681 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     1741 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     1801 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     1861 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     1921 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     1981 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     2041 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     2101 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     2161 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     2221 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     2281 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     2341 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     2401 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     2461 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     2521 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     2581 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     2641 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     2701 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     2761 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     2821 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     2881 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     2941 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     3001 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     3061 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     3121 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     3181 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     3241 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     3301 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     3361 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     3421 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     3481 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     3541 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     3601 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     3661 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     3721 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     3781 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     3841 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     3901 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     3961 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     4021 tttctactat caacaaggag gagcagaaac tgtg
//
LOCUS       DAZOZD010000312         4052 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOZD010000312 DAZOZD010000000
VERSION     DAZOZD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.315"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            47..1561
                     /gene="caiT"
                     /locus_tag="ACHUPB_004104"
                     /pseudo
     CDS             47..1561
                     /gene="caiT"
                     /locus_tag="ACHUPB_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            1592..2734
                     /gene="caiA"
                     /locus_tag="ACHUPB_004105"
     CDS             1592..2734
                     /gene="caiA"
                     /locus_tag="ACHUPB_004105"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0760762.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2863..4052
                     /gene="caiB"
                     /locus_tag="ACHUPB_004106"
                     /pseudo
     CDS             2863..4052
                     /gene="caiB"
                     /locus_tag="ACHUPB_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT          948 a         1034 c         1086 g          984 t
ORIGIN      
        1 aacgggaacg caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa
       61 gagaaaaacg ggaatagaac cgaaggtttt ctttccgccg ttaataatcg tcggcatact
      121 ttgttggctt acagtcagag atctggatgc agcgaatgtc gttattaatg ctgtattcag
      181 ttacgtcacc aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg
      241 ttggttctgg ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccacctga
      301 atttagtacc gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact
      361 gttctgggga tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc
      421 gaactcgaca ggggcgaaag agttggggct ggcttacagc ttgttccact ggggacctct
      481 gccgtgggcc acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa
      541 aatggaagtg attcgcccca gctcgacact ggtgccgctg gtaggtgaaa aacacgccaa
      601 agggttgttc ggcactatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg
      661 taccagtctg ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat
      721 tccgcatacc ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat
      781 ttgcgtcgct tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct
      841 gagcttcctg atgctgggtt gggtgttcat tgtcagcggt gccagcttca tcatgaacta
      901 cttcaccgat tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga
      961 tcccatcgct aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt
     1021 gatttatgct atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg
     1081 tgaactgtgc ttcggcatgg tgctggggct gacagcgtca acctggatcc tgtggactgt
     1141 actcggtagt aacactctgc tgttgataga taaaaacatc atcaacattc caaatctgat
     1201 cgaacagtac ggtgtggcgc gcgccatcat tgaaacctag gccgctctgc cactcagcac
     1261 cgccaccatg tggggcttct tcatcctctg ctttattgcc accgttacac tggttaacgc
     1321 ctgctcttat accctggcga tgtccacttg ccgcgaagta cgcgatggtg aagaaccacc
     1381 tctgctggtg cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc
     1441 gctcggcggc ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt
     1501 cgtcaacatt atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta
     1561 attaaccccc aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga
     1621 actgtttgtc gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc
     1681 cgagtgcgac cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg
     1741 tatcgacagt ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct
     1801 cgccgccgtg tggatggagc tgggacgtct gggggcacca acctatgtgc tgtaccagtt
     1861 gccgggcggg ttcaacacct tcctgcgcga aggcacacaa gagcagatcg acaaaattat
     1921 ggctttccgc ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg
     1981 ctccgacgtg ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa
     2041 tggtagtaag tgttttatta ccagcagcgc ctacaccccg tacatcgtgg tgatggcgcg
     2101 cgacggggct tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc
     2161 gggcatcaaa gtgaccaaac ttgaaaagct cggtctgcgt atggatagct gctgtgaaat
     2221 cacctttgac gacgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt
     2281 taaccgcgtc aaagaagagt tcgaccatga acgtttcctg gtagccctca ccaactacgg
     2341 tacggcgatg tgcgcttttg aagatgcggc gcgctacgcc aatcagcgcg tgcagtttgg
     2401 cgaggctatt ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt
     2461 aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac
     2521 ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagttgtgga
     2581 tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt
     2641 ctggcgtgat ctgcgtgtag accgcgtctc cgggggatct gacgaaatgc agatcctgac
     2701 gctgggtcgc gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct
     2761 aatgcgtctt atcaggccta cgggagcacg aatgtaggcc ggataaagcg tttacgccgc
     2821 atccggcagt catgcactac ataaattttt caggagctaa taatggatca tctacccatg
     2881 ccgaaattcg ggccgttggc cggattgcgc gttgtcttct ccggtatcga aatcgccggg
     2941 ccgttcgccg ggcaaatgtt cgcagaatgg ggtgcggaag ttatctggat cgagaacgtc
     3001 gcctaggccg acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg
     3061 cacgcgctgt cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg
     3121 gaaaccaccg atatcttcat cgaagccagc aaaggtccgg cctttgcccg tcgtggcatt
     3181 accgatgaag tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt
     3241 ggtcagtacg gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc
     3301 ttcagtggtt acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat
     3361 accgccgatt acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa
     3421 gtgcgtgaaa ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt
     3481 atgggccagt acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc
     3541 aaaggtaaag atccctacta cgccggttgc ggtctgtata aatgcgccga cggctacatc
     3601 gtgatggaac tggtgggcat tacccaaatt gaagagtgct ttaaagatat tgacctcgca
     3661 catctactcg gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc
     3721 ccttacggcc cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca
     3781 gaagttaaag aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg
     3841 gaactggaaa gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg
     3901 gatggtcgca cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa
     3961 atctggcgcg gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc
     4021 tacagcgaaa acgacattca ggagttggtc ag
//
LOCUS       DAZOZD010000313         4045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOZD010000313 DAZOZD010000000
VERSION     DAZOZD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.316"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHUPB_004107"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHUPB_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0760763.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUPB_004108"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUPB_004108"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0760764.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUPB_004109"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUPB_004109"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0760765.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUPB_004110"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUPB_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0760766.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1142 c          970 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAZOZD010000314         4038 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOZD010000314 DAZOZD010000000
VERSION     DAZOZD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.318"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            150..851
                     /locus_tag="ACHUPB_004111"
     CDS             150..851
                     /locus_tag="ACHUPB_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0760767.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1109..3256)
                     /gene="mobQ"
                     /locus_tag="ACHUPB_004112"
     CDS             complement(1109..3256)
                     /gene="mobQ"
                     /locus_tag="ACHUPB_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR0760768.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3435..3689
                     /gene="mobC"
                     /locus_tag="ACHUPB_004113"
     CDS             3435..3689
                     /gene="mobC"
                     /locus_tag="ACHUPB_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR0760769.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          957 a         1027 c         1051 g         1003 t
ORIGIN      
        1 gggggtgaaa ggtggagctt ttgggggtga aaggtggagc ttttgggggt gtaatccaca
       61 gaaaaagggg gttgcaaaaa aaaaacttcc acccatatga taagtggcgt acagtaaaaa
      121 aacgccacca atagcaacga gcaagcgaaa tgtcagatct gatagcttac aaatcaaacg
      181 cccttatcga ggcaagctac aagctcactt tgcaggaaca gcgcttcctg ctggtctgta
      241 tcagccgcct gaaatccgga gaagattccc cttcgccgga tgaacaaaaa accatgacca
      301 tcacggctgc agagtttcat gatgcctttc ctgacatggg acggcagcac gctgaagcta
      361 ggctcaagga agcgatagat cgcctctggg acaggtcagt aatcatcaag aacgaggaaa
      421 aacgagaaga gttccgctgg gtgcagtacc gggcgcagta tttcaaaggt gaggcgaaag
      481 tagatatcac cttctccgat gccatcatgc cgtatctgac acagctcaag gggcagttta
      541 cgcgggtagt cgtgaagaac gtctccagcc tttccagcac ctacagcatc cgcatttacg
      601 agctgctcca gcagttccgc agcaccggtg acagaacgat agctatcgat gatttccgaa
      661 ccatgcttgc cgttgaagat aaatatcaac aattcaaaga cttgaataag atgataatca
      721 agccagccat cgccgaactg aacgaaaaaa gcgatctggt tgtaactgtt gagacgatca
      781 aaaaggggcg cacggttgta gccctgcatt ttcgcttcaa ggaagacaaa cagatcaaga
      841 tgacgatcta aaatcgattt taaggcacct aaaacgctcc ctgctgcgtt ttttttgtgt
      901 tagtagtgcg aaggtactat tgaagtgaaa aattgcgtca aaaacgctct gatgcagcga
      961 aaaaagaccc cccaattttt gggggcggaa aaacaaaact atgcttgcgt tatgtgagtg
     1021 tgcttcttgc attcatccct gtcgatgaac atcgaagaac atcgaagaac ataacacgat
     1081 gttaaacaaa acaaaatatt gttcacattc actgtgacca accgcgatca tgtctcctct
     1141 cggtggtgtg tgttcgtgca tcttcctcgc gctgccattc ctcccaggta cgattaaaac
     1201 ccagcgagcg gaacagatga tccggctgtt tttctggtgt gttgacggca gcaaaaagcg
     1261 gtgcctcacg ggttttcaga tgaaactgca tcagttctgg tgcaggtgat atgtggctgt
     1321 ctgcatcgtg atttacggca acaccatacc ctgcataccg taacccctta tcggtcagcg
     1381 cactgatgac cagatgcctg ttatcggctg agggaacggc aaacgcggga tacagcgtac
     1441 cattaagccg gataacaccg cccagagagt catccacgcc catgaccagc aaaagctgaa
     1501 gctggcgggt ttcccggtcg acagaactca tgaccggcat cagcgtcggt ggcagacgtc
     1561 cctgcataaa atccaccttc cttgccgctg gtgatggcat ggctggctgg cggtcttctc
     1621 caagacagaa agactccggc catgtcttcg gctggcggag attgcgggta tgttgcggcc
     1681 atgtctggca aacctgcgtc agcagttgct caaaggttgc agaggaataa acacgttgca
     1741 gtggcggacg ttggccgatt tcgcttcggt cacagatata accactcctg tcacgcatta
     1801 aatcaacact ctcaaagatt ctggtgcggt atttctccag ctcgttcccc cagagcgtat
     1861 attctccgtt ccggttgtga agcagaaggt aacaggtcat ttccccgtta ccaggcatgg
     1921 gagcctcgcc ataccccgta acgactgctc tcagcacccc tgaatccata acggcattgt
     1981 catttttcct gagctgctcc gcgcgactac gttcagtatc ttcctgttcc ttccggcgca
     2041 attcctcttc acgtttaagc aactgggctc tgagagctgc aaggccggat tccccccact
     2101 gctgccactg tttttcaaat gccatctgac tcgcgggaat gtcctgtttc tccaccttcc
     2161 ggtaatgctg tcgtaatttt tccatttgct ggctcagaga ctggattttt ccctgctcac
     2221 tacggtattc cggtgccgcc cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg
     2281 gcgatgattt tttcaggctg gcagcatggc tcatcgcttc ctttcgttcg gtgttcagct
     2341 cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat cgacatggca ttactggtga
     2401 gtttccatgc cgagttactg tccttcggca acgcttcgat aatgtcacgt agtgtgtacg
     2461 aagttgcgtt gtcaggaagc ccgatattca gaaggtcagc cacacggcgg gcaggctctg
     2521 tcagccctcc gttcctgagt tcggtgcggg aaatctgtat ttccaggtta agcctgttaa
     2581 tcagacgatt ctgttcgccg cgttctgttt cgataccgcg tgtttccatc gcacaggcag
     2641 actttccgag gtgaatacca ggcgttttct caagcccctg tcgctcgtat gaacgatggt
     2701 ctatctcttc ctgataaccg gcgttcgcca gtgccctgtt tgcatggtca gcccatgatg
     2761 cccgccacgt ctcggcatgt gtccggtcgt tccagtccct gacctttccg ctgaacccag
     2821 caggtccgat agccctcgtg gtcagcatga tgtgcgcatg gggatttgtt ttatccatgt
     2881 gatggaacgc cacatcagca atcatgccct gactgacaaa gttttctctg acaaaggcga
     2941 gaacggtttc ccgtgcggca tcctgcggca gttcgtgggg gatggcgagt tcaatttccc
     3001 gcgcaagttg tgagttcttc cggcgttcgg ccttctcaac ggcattccag agttcggcac
     3061 ggttcgtaca ccaggcaggg gcggtggcgg gggtcagaat cacagcctca gccacaccgc
     3121 ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc aagcatcagt gaaccggaac
     3181 gataggcagc ggaagctacg gcgctgtagc cgctgcttct ggagataatt ttcatgctga
     3241 gatgataaat cgccatttct gccgtaatgc ctttttgtag ttttctttca gccccgcagg
     3301 gcgcacacaa tcgaaacgga gtggagattg tataagtgcg cacttcgtga tttgccgtgc
     3361 attatgccag ctcagttttt aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg
     3421 cacggagtta catcatgagc agaacgctcg aacagaagat tgctgatgct gaggcgagat
     3481 tacagcgcct gaaggcgaag agccggagcc tggacacagc gcagaaggtt gttgtgggtg
     3541 ctgcattgct ggcaaaggtc agaaagccgg aggaggtgca gttgcgtgcc tggttactcc
     3601 agttcctgaa ggcagaggtg acacgacagg ctgatgtgac gcgcatactg ccgctgatta
     3661 acgagctgga ggcgctgcca gaacagtgat tgtatcgtcc gctcgccgca gccccagcga
     3721 ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga atcagtcgcc aggttgagca
     3781 catacgcacg gtttttcggc aggtgatatg cgctgaaccc gcatgaatac tgggtttcca
     3841 cagggttgga gggggtgtat tcgaaacttg caattctgct ttatttcaac gcattgtttt
     3901 tattggtttt ttgttttcag caccccaagt tatccacagg ctcgccgtta agtttttaag
     3961 atcttatttt tagatctttt ttttaaaacc tttttaggtc ttgtaacacc atgattttaa
     4021 agatgttctg gcaagata
//
LOCUS       DAZOZD010000315         3992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOZD010000315 DAZOZD010000000
VERSION     DAZOZD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.319"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(207..1478)
                     /gene="efeB"
                     /locus_tag="ACHUPB_004114"
     CDS             complement(207..1478)
                     /gene="efeB"
                     /locus_tag="ACHUPB_004114"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0760770.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(1484..2611)
                     /gene="efeO"
                     /locus_tag="ACHUPB_004115"
     CDS             complement(1484..2611)
                     /gene="efeO"
                     /locus_tag="ACHUPB_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0760771.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(2669..3499)
                     /gene="efeU"
                     /locus_tag="ACHUPB_004116"
     CDS             complement(2669..3499)
                     /gene="efeU"
                     /locus_tag="ACHUPB_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0760772.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT          981 a         1114 c          959 g          938 t
ORIGIN      
        1 ctactggaaa gatgacagag tgatgacagt gatgaaaaaa gctgtgtgct ttcagcagga
       61 tttgcatcag gcatcagtgc acgatcgccg gatgcggcgt gaacgcctta tccagcctac
      121 caaacccgca ctgattgtag gcatgataag acgcgcaagc gtcgcatcag gcatctgcgc
      181 acaaatgcct tatccgccta aaaacattaa acccgcaata acgcgcttcc gagataatcg
      241 ttcgcgtcct tcactcccgg cagcgaaaaa aaataaccgc cgccgatagg tttaacgtat
      301 tcctccagcg cttcgccatt gagccttttt tgtactgtca ggaagccttt ttccagatcg
      361 tgttggtagc agacaaacag caaccccata tccagttgtc cggagttggt gacccccaac
      421 gaataactgt agccgcgacg cagcatcagg ctggactcac tctccgccgt gcggggattc
      481 gccagccgga tatggctgtc cagcgcaatc accttccctt ccgggtcgct ggcgtaatca
      541 ggcacatcat gctcatgctg cattcccagc ggcgcaccgg tttgcttatc acggccaaaa
      601 atcgtctgct gttctttcag cggcgttctg tcccaaaatt ccactcgaaa ctgaatcaag
      661 cgtactgcct gatagctgcc accgattgtc cacgcaggct cctgctgatc tgccgttacc
      721 cacaccactt tttgcatcaa cttatcattc tggctatcgg gattggcagt gccgtctttg
      781 aaacccagca aattaatcgg cgtctctttg cctttactac gcgacacgtg atcggaaata
      841 aacccttccc gcttccagcg cacactgagc aaatccggcg tgtgtttgat gatatcgcgc
      901 agcgcatgga taaccgtgtc ctgggtgttg gcgcaaatct gtagcaacac atcaccatga
      961 cataacgccg catccagcga gtcgttgggg aaacgcgtca tcttctgcag cttttttggc
     1021 atctgtggcg caaggccaaa gcgctcatca aacaatgagt gacccaccga taacgtgatg
     1081 gtgagattat cgggcgcaat gtagccgcca agaatgccgg aatcgagtgg tggcaggcgc
     1141 ggatttggcg tttctggtgc tgctccgccc tgagtcagaa aagcaaaacg ctgagtcaac
     1201 aagcgaaaca accgctcaag atcggcttta tcgctggcaa gcacatcaaa cgccaccagc
     1261 atcattgcgg cctgttgtgg cgtcaggatc cctgcctgat gctcaccata aaacggctgt
     1321 ttctcattgc gtgcatccgg tgaaagcgtg cccggtgcac tttgcgtttt ttgtgcatga
     1381 gcgaccggac aacttcccgc cagcgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     1441 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     1501 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggtcctttc agtgcattcc
     1561 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     1621 gaatggtatc gaccttttta aagttggcat cgacttttgc cagcagttcc gggttggctt
     1681 tttgcagttg tggacgcagc aaatcgacaa ttttctgcga gccttcaacg ttagcctgga
     1741 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctaattttg ctggctgcca
     1801 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     1861 tgatgcgttt ttgcaaatcg accacatctg tataaagctg ctcagcgtac tgatccatcc
     1921 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     1981 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     2041 agaacagttc agcaatcggt tcaatgcgct catagtgctg gcgcgtcggc gcatacagtg
     2101 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     2161 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     2221 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     2281 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     2341 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     2401 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     2461 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     2521 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     2581 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     2641 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     2701 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     2761 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     2821 agtgagttga gagcactgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     2881 cggcttcatg aaatgcgcga atggcaccag cagccagccc tgcggcgacg aagagaataa
     2941 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     3001 ggaagcctag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     3061 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     3121 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     3181 tatcgactgc ctgctccagt tgtactttga cgttgcgcga cactttgcgc atccagaaaa
     3241 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgtt
     3301 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat acccaagccc aggcacaacg
     3361 cagcggcaag caacacgcca atccacatca cgccaatcca tcggcctcgc tgggtacgct
     3421 taagatagct ggcaatcaaa ctgacaatca gcgcggcttc aagtccttcg cgcaacataa
     3481 tgagaaacgg aacaaacatg ccacccaccc ttaaacgttt ttctgagtca atccatgcaa
     3541 agaaaagtaa atcgcagtga tagcgattat cattacggca aagaaaaata caagcggaat
     3601 gtttataaaa tgatgttgga atgtaaacaa agagaggtaa taccagggga tataaacgat
     3661 tattccaggt acaaagatgg gattgtttac aacgagccat gtgttcttgc cagatgccgc
     3721 gtaaacgcct tatccagccg acaaaactgt ccagactcaa tgcatcacag cttccatgta
     3781 aaactgataa gcgggtgtat caagcaattt cgtccatccg cgattatcaa atctaacttt
     3841 ttgcccggaa cataataaca gcgcgggtgt tggtggcgtc aaaatcgctg aaacgttccc
     3901 cgaaggccgg ggcagcccac atggatgtgg gctgagggcg cgtcttacag gacgttacct
     3961 cgcgcccgac ccggtagccg aaggggataa gc
//
LOCUS       DAZOZD010000316         3950 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOZD010000316 DAZOZD010000000
VERSION     DAZOZD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.320"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUPB_004117"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUPB_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR0760773.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUPB_004118"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUPB_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR0760774.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUPB_004119"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUPB_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR0760775.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUPB_004120"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUPB_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR0760776.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1008 c         1001 g          918 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag
//
LOCUS       DAZOZD010000317         3919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZOZD010000317 DAZOZD010000000
VERSION     DAZOZD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.321"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHUPB_004121"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHUPB_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHUPB_004122"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHUPB_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR0760777.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          948 a         1036 c          973 g          962 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataa
//
LOCUS       DAZOZD010000318         3909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOZD010000318 DAZOZD010000000
VERSION     DAZOZD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.322"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            25..591
                     /locus_tag="ACHUPB_004123"
     CDS             25..591
                     /locus_tag="ACHUPB_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0760778.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            595..1170
                     /locus_tag="ACHUPB_004124"
     CDS             595..1170
                     /locus_tag="ACHUPB_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0760779.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1212..2264)
                     /locus_tag="ACHUPB_004125"
     CDS             complement(1212..2264)
                     /locus_tag="ACHUPB_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0760780.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2489..3409
                     /gene="lpxL"
                     /locus_tag="ACHUPB_004126"
     CDS             2489..3409
                     /gene="lpxL"
                     /locus_tag="ACHUPB_004126"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0760781.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3581..3766
                     /locus_tag="ACHUPB_004127"
                     /pseudo
     CDS             3581..3766
                     /locus_tag="ACHUPB_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          988 c          951 g          999 t
ORIGIN      
        1 cgcaaattcg tattggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt
       61 atatctgcgg ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt
      121 ctgtggatgg tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat
      181 aaaagcatcg gtattttgtt aatgatgggg ctggttattc gcgttctgtg gcgtgtcata
      241 tctccaccgc cgggtccgct gccgagttat tcgcccatga ctcgtcttgc cgcaaaggct
      301 ggacatctcg cattgtacct attgctgttc gccatcggca tcagcggcta tctgatctca
      361 actgccgatg gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gacccttgcc
      421 gacgctggcg cacaggcaga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc
      481 gtagtactgt ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac
      541 gacactctga agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa
      601 aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct ctgccggttc agcggttgcc
      661 gccgattaca aaattgacaa agaaggtcag cacgcctttg ttaatttccg catccagcac
      721 cttggctata gctggttata cggcaccttt aaagatttcg acggtacttt tacctttgac
      781 gaaaaaaatc cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact
      841 aatcacgccg aacgcgataa acatcttcgc agtgcagatt tcctcaatac cgcaaaatat
      901 ccacaggcaa cattcacctc caccagcgtg aagaaagacg gtgacgaact ggatattacc
      961 ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt
     1021 cagggcgacg acccatgggg tggtaaacgt gcaggcttcg aggccgaagg caaaattaag
     1081 ctcaaggact tcaatatcaa gacagattta ggtccagctt ctcaggaagt ggatctgatt
     1141 atttcagtgg aaggcgtaca gcagaagtaa gcgtgaaatg ttgccggatg gcacgcatcc
     1201 ggcaatgata tttattctgt tggatcagga atgcccagtg ttgtattcag acgtccacga
     1261 gacttattaa agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttct
     1321 ggcggtaacg cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgcg
     1381 catactggac actgaataaa cagcaggtgg cagccatcat ttttacagtt ggtatggctg
     1441 tcgcacggcg cgccgcactg gtggcaatgc gcgataatct catccgatat acgttcgccc
     1501 atccgctcgt caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc
     1561 gccttacggg cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta
     1621 tgtttcatcc aggcactggc cttttcacaa cgaatgccgc cggtgcagta catgacgatt
     1681 tttttatctt tatgtgcctg catcatctcg actgcttttg gcagctgctc acggaaggta
     1741 tctgccggaa tttcgagcgc gttttcaaag tgccccactt catactcata gtggttacgc
     1801 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
     1861 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
     1921 tcgcgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
     1981 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
     2041 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
     2101 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
     2161 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcaac caacattttg
     2221 gcttttaacg cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc
     2281 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
     2341 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
     2401 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
     2461 cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
     2521 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca
     2581 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
     2641 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
     2701 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggactgat
     2761 ggaaaccggt atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
     2821 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
     2881 ccattttctg acactggaac tgggcgcgcg gcagtttggt attcaggaac cggggattgg
     2941 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
     3001 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
     3061 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
     3121 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
     3181 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
     3241 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
     3301 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     3361 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc
     3421 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
     3481 agctctctgg attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt atcttttctt
     3541 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtggaaatga
     3601 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     3661 tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     3721 cggtcactcc gccctgaata tgtggtccgg tattgtcttc agtataatac cttcctaaat
     3781 acactacctg agagaagaga tgaaaagagc tatcagctac atgaggtttt caactgagaa
     3841 gcagataaaa ggggactcta tacgaagaca gtctaagctg gtcactgact ggttatcaaa
     3901 gaaccctga
//
LOCUS       DAZOZD010000319         3858 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOZD010000319 DAZOZD010000000
VERSION     DAZOZD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.323"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHUPB_004128"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHUPB_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0760782.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUPB_004129"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUPB_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0760783.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUPB_004130"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUPB_004130"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0760784.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUPB_004131"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUPB_004131"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0760785.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAZOZD010000320         3840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOZD010000320 DAZOZD010000000
VERSION     DAZOZD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.324"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..288)
                     /gene="chbB"
                     /locus_tag="ACHUPB_004132"
     CDS             complement(<1..288)
                     /gene="chbB"
                     /locus_tag="ACHUPB_004132"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0760786.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAA"
     gene            complement(587..925)
                     /gene="osmE"
                     /locus_tag="ACHUPB_004133"
     CDS             complement(587..925)
                     /gene="osmE"
                     /locus_tag="ACHUPB_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0760787.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1127..1954
                     /gene="nadE"
                     /locus_tag="ACHUPB_004134"
     CDS             1127..1954
                     /gene="nadE"
                     /locus_tag="ACHUPB_004134"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0760788.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2184..3071
                     /gene="cho"
                     /locus_tag="ACHUPB_004135"
     CDS             2184..3071
                     /gene="cho"
                     /locus_tag="ACHUPB_004135"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0760789.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3031..3606)
                     /gene="ves"
                     /locus_tag="ACHUPB_004136"
     CDS             complement(3031..3606)
                     /gene="ves"
                     /locus_tag="ACHUPB_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0760790.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          980 a          953 c          918 g          989 t
ORIGIN      
        1 cgcagcctta agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc
       61 aaccggtttg ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc
      121 taataacacg acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc
      181 aataatgacc ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga
      241 ggtagacatg cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc
      301 atcgacgatt atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg
      361 taacttgcga aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg
      421 agcaatagta tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc
      481 ttcacatttc gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg
      541 cgcaatggcc ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct
      601 gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat
      661 catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct
      721 ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg
      781 caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt
      841 ctttagtacg atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca
      901 gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag
      961 cctggaatac attgggcgct ttttcaagcc cgcgaacgaa acggctccgc tttcagagga
     1021 ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtggcaac
     1081 gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca
     1141 acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg
     1201 tagtatcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct
     1261 cgggatcagc ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa
     1321 tgagctgcgc ctggaaaccg gcaacgaatc actgcaattt attgccgtac gcctgcccta
     1381 tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg
     1441 cgtattaacc gttaatatca agggcgcggt attggccagc gaacaggcat tgcgggaagc
     1501 aggcattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc
     1561 acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga
     1621 agccattacc ggattcttca ctaaatatgg tgacggcggt acagacatta atccgctgta
     1681 tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct
     1741 ttataagaaa gcgccaacgg ccgatctgga agatgatcgc ccttctctgc cagatgaagt
     1801 ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga
     1861 acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc
     1921 aattaccgtt ttcgatgatt tctggaaaaa gtaataattt gcacataatg gtttgtgcga
     1981 aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta
     2041 tgcaaattca atatattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt
     2101 gtaacctttg tcatcgaccc gcctcttttt taatcgctcc ccgcctgtta cactggatag
     2161 ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg
     2221 aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca
     2281 cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca
     2341 aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca
     2401 tgctacggca atcccggcga attagctgga tctgtaccgc aggcgaaatc ggcgctctgc
     2461 tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca
     2521 atcgccagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag
     2581 aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt
     2641 tgcaagcatt gcagaccatc gctgatgaac aaaaactttg ttatggcctg ctgggactgg
     2701 aaccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat
     2761 gctgcggtaa agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt
     2821 tgcgggtggt gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa
     2881 tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag
     2941 aagcgacaac gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt
     3001 gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc
     3061 gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg
     3121 cctgccatcg aaccagcacg cgccctggtc ggtagtgagc aatttatcgc ccagttgcca
     3181 cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc
     3241 ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga
     3301 catctgccct tccgtcagtt tcgcttttac cacctggtcc gctgcaaagg caaaaggctg
     3361 taacggtttt aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc
     3421 cagcaacgtc actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat
     3481 ggatgcaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc
     3541 gcgcgtttca ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta
     3601 ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt
     3661 tatcggcaac tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag
     3721 aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc
     3781 ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg
//
LOCUS       DAZOZD010000321         3828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOZD010000321 DAZOZD010000000
VERSION     DAZOZD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.325"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..2893
                     /gene="sigA"
                     /locus_tag="ACHUPB_004137"
     CDS             <1..2893
                     /gene="sigA"
                     /locus_tag="ACHUPB_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0760791.1"
                     /translation="SSIIKDKDLSVSGGGELTLKQDTDLGIGGLIFDKNQTYKVYGKD
                     KSYKGAGIDIDNNTTVEWNVKGVAGDNLHKIGSGTLDVKIAQGNNLKIGNGTVILSAE
                     KAFNKIYMAGGKGTVKINAKDALSESGNGEIYFTRNGGTLDLNGYDQSFQKIAATDAG
                     TTVTNSNVKQSTLSLTNTDAYMYHGNVSGNISINHIINTTQQHNNNANLIFDGSVDIK
                     NDISVRNAQLTLQGHATEHAIFKEGNNNCPIPFLCQKDYSAAIKDQESTVNKRYNTEY
                     KSNNQIASFSQPDWESRKFNFRKLNLENATLSIGRDANVKGHIEAKNSQIVLGNKTAY
                     IDMFSGRNITGEGFGFRQQLRSGDSAGESSFNGSLSAQNSKITVGDKSTVTMTGALSL
                     INTDLIINKGATVTAQGKMYVDKAIELAGTLTLTGTPTENNKYSPAIYMSDGYNMTED
                     GATLKAQNYAWVNGNIKSDKKASILFGVDQYKEDNLDKTTHTPLATGLLGGFDTSYTG
                     GIDAPAASASMYNTLWRVNGQSALQSLKTRDSLLLFSNIENSGFHTVTVNTLDATNTA
                     VIMRADLSQSVNQSDKLIVKNQLTGSNNSLSVDIQKVGNNNSGLNVDLITAPKGSNKE
                     IFKASTQAIGFSNISPVISTKEDQEHTTWTLTGYKVAENTASSGAAKSYMSGNYKAFL
                     TEVNNLNKRMGDLRDTNGEAGAWARIMSGAGSASSGYSDNYTHVQIGVDKKHELDGLD
                     LFTGLTMTYTDSHASSNAFSGKTKSVGAGLYASAIFDSGAYIDLISKYVHHDNEYSAT
                     FAGLGTKDYSSHSLYVGAEAGYRYHVTEDSWIEPQAELVYGAVSGKRFDWQDRGMSVT
                     MKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIK
                     GEKDGRILMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            2952..3521
                     /locus_tag="ACHUPB_004138"
     CDS             2952..3521
                     /locus_tag="ACHUPB_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760792.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTDTITE
                     ATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLFQGKM
                     STDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT         1215 a          806 c          846 g          961 t
ORIGIN      
        1 atcatcgatt ataaaagata aggacctgtc tgtctcagga ggaggggaat taacgctcaa
       61 gcaggatacc gatcttggca ttggcgggct tatattcgat aagaaccaga catataaagt
      121 gtacggaaaa gataagtctt ataaaggtgc cgggatagat attgataata ataccaccgt
      181 tgaatggaat gttaagggcg ttgccggaga taatctgcat aaaataggta gtggtactct
      241 ggatgtaaaa atagcacagg gaaataacct taaaataggt aatgggactg tcatccttag
      301 tgctgaaaaa gccttcaata aaatttacat ggccggaggt aaaggtacgg taaaaataaa
      361 tgccaaagac gctttaagcg aaagcggtaa tggcgaaatc tattttacca gaaatggcgg
      421 aacactggat ctaaacggct atgaccagtc atttcagaaa atcgcagcaa cagatgcggg
      481 aacaaccgta acgaactcaa acgtgaagca atcaacatta tcacttacta atactgatgc
      541 atatatgtac catgggaatg tatcaggtaa tataagcata aatcatatta tcaatactac
      601 ccagcaacat aacaataatg ccaatctgat ctttgatggc tcagtcgata tcaaaaacga
      661 tatctctgtc cggaatgcac agttaacatt acaaggacat gcgacagaac atgccatatt
      721 taaagaaggc aataacaact gtccaattcc ttttttatgt caaaaagact attctgctgc
      781 cataaaggac caggaaagca ctgtaaataa acgttacaat acggaatata agtccaacaa
      841 tcagatagcc tctttttccc agcccgactg ggaaagtcgt aaatttaatt tccggaaatt
      901 aaatttagaa aacgcaaccc tgagtatagg ccgggatgct aatgtaaaag gacacataga
      961 ggctaaaaac tctcaaattg ttctgggaaa taaaactgca tacattgaca tgttctcagg
     1021 aagaaacatt actggcgaag gttttggatt cagacaacag cttcgctccg gggattcagc
     1081 aggcgaaagt agtttcaacg gcagtctgag tgctcaaaac agcaaaataa ctgttggtga
     1141 taaatcaact gttactatga ctggtgcatt atccttaatt aatacagacc tgattatcaa
     1201 caaaggagct actgttaccg cccagggaaa aatgtatgta gataaagcta ttgaactggc
     1261 cggaaccctg acattaacag gcacccctac agaaaataat aaatacagcc cggcaatcta
     1321 tatgtcagat ggatataata tgacagaaga tggtgccacg ttaaaggctc aaaattatgc
     1381 ctgggtcaat ggtaatataa aatcagacaa aaaagcatct attctgtttg gtgttgacca
     1441 gtataaagaa gataacctgg acaaaaccac acacacaccg ctggctacag gtttgctggg
     1501 tggctttgat acttcttata ccggaggtat tgatgctcct gctgcctcag ccagcatgta
     1561 taacacctta tggagagtaa acggacagtc agccctgcaa tcattaaaaa cccgcgacag
     1621 tcttttgttg tttagtaaca tagagaattc gggtttccat actgtgacag taaacacact
     1681 ggatgccact aatactgctg tgattatgcg ggctgatctg agccagtctg taaatcaatc
     1741 ggataaactc attgttaaaa atcagttaac cggaagcaat aacagtctgt cggtcgatat
     1801 acagaaagtg ggaaataata actcaggatt aaacgttgac ctgataacag ccccaaaagg
     1861 aagcaataaa gagatattta aagccagtac tcaggccata ggtttcagca acatatctcc
     1921 tgtgatcagc acgaaagagg atcaggaaca taccacgtgg accctgaccg gatataaggt
     1981 ggctgaaaat acagcatctt ccggtgcagc aaaatcgtat atgtccggta attacaaagc
     2041 cttcctgaca gaagtcaaca acctgaataa acgaatgggg gatctgcgtg acaccaatgg
     2101 cgaggccggt gcatgggccc gcatcatgag cggagcaggt tcagcttcta gtggatacag
     2161 tgacaactac acccatgtgc agattggtgt ggataaaaaa catgagctgg atggacttga
     2221 ccttttcact ggtctgacta tgacgtatac cgacagtcat gccagcagta atgcattcag
     2281 tggcaagacg aagtccgtcg gggcaggtct gtatgcttcc gctatatttg actctggtgc
     2341 ctatatcgac ctgattagta agtatgttca ccatgataat gagtactcgg cgacctttgc
     2401 tggactcgga acaaaagact acagttctca ttccttgtat gtgggtgctg aagcaggcta
     2461 ccgctatcat gtaacagaag actcctggat tgagccgcag gcagaactgg tttatggggc
     2521 cgtatcaggt aaacggttcg actggcagga tcgcggaatg agcgtgacca tgaaggataa
     2581 ggactttaat ccgctgattg ggcgtaccgg tgttgatgtg ggtaaatcct tctccggtaa
     2641 ggactggaaa gtcacagccc gcgccggcct tggctaccag tttgacctgt ttgccaacgg
     2701 tgaaaccgta ctgcgtgatg cgtccggtga gaaacgtatc aaaggtgaaa aagacggtcg
     2761 tattctcatg aatgttggtc tcaacgccga aattcgcgat aatcttcgct tcggtcttga
     2821 gtttgagaaa tcggcatttg gtaaatacaa cgtggataac gcgatcaacg ccaacttccg
     2881 ttactctttc tgataacagc ccgggccgcg tttgcggccc ttcttctacc gtagagaata
     2941 tgtattaccc tgtgacagac tatatcgctc ttgctctcat tattagcttt ctttttctga
     3001 cattatttat ctgcctgtta tgccttaaac atgagcgaat aaaaaaagaa actatcaggc
     3061 aaaaaaatgc acatattctg gagcatggct ggaatgcaac tgagttctca tggttccgat
     3121 acggacagta taacgaaacg ggtatttaca tctcgatcga gaagaccatt atcattacaa
     3181 tcaccgtttc tggtgagtgc tttaaaaaag aatacagcat tgtctcccac atgctggtca
     3241 ctgacaccat aacagaggcc accctgtacg aaaacgggct gtatacgcgc cacatcagac
     3301 tcagtcgccc agtatctgac agcaaatacc ccttgccacc cggatctcag ctcattaaaa
     3361 atatgacctt acgactgcgc ctgcaggatc agcaggaaga aacatccgtg acattatttc
     3421 agggaaaaat gagtaccgac ggaaataact attacatcat caaaggtaaa gtaagctcag
     3481 tacttctgtt attaaaaatg ctgcagatta atcacgcctg atctctgtcc cggtatttta
     3541 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
     3601 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
     3661 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
     3721 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
     3781 gtaagcgtaa accgactgcc gtatgtagcc attaagccag tattggta
//
LOCUS       DAZOZD010000322         3824 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOZD010000322 DAZOZD010000000
VERSION     DAZOZD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.326"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..3239
                     /gene="icsA"
                     /locus_tag="ACHUPB_004139"
                     /gene_synonym="virG"
     CDS             <1..3239
                     /gene="icsA"
                     /locus_tag="ACHUPB_004139"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR0760793.1"
                     /translation="VKEKTCKLSFSPFVVGASLLLGGPIAFAIPLSGTQELHFSEDNY
                     EKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHASRTKLRINSDIMISAGHGGAGDNND
                     GNSCGGNGGDSITGSDLSIINQGMILGGNGGSGADHNGDGGEAVTGDNLFIINGEIIS
                     GGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTISGGNGGNNYGEGDGGNGGDAITGSS
                     LSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDNLSIINNGAILGGNGGHWGDAINGS
                     NMTIANSGYIISGKEDDGTQNVVGNAIHITGGNNSLILHEGSVITGDVQVNNSSILKI
                     INNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVSGDVSFGENSSLNLAGISSLEASGN
                     MSFGNNVKVEAIINNWAQKDYKLLSADKGITGFSVSNISIINPLLTTGAIDYTKSYIS
                     DQNKLIYGLSWNDTDGDSHGEFNLKENAELTVSTILADNLSHHNINSWDGKSLTKSGE
                     GTLILAEKNTYSGFTNINAGILKMGTVEAMTRTAGVIVNKGATLNFSGMNQTVNTLLN
                     SGTVLINNINAPFLPDPVIVTGNMTLEKNGHVILNNSSSNVGQTYVQKGNWHGKGGIL
                     SLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEGGSGDKTLEGVQIISTDSSDKNAFI
                     QKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMDNQESKQMSNQESTQMSSRRASSQL
                     VSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSPSLYDRHGSTIVDPTTGQLSETTMW
                     IRTVGGHNEHNLADRQLKTTANRMVYQIGGDILKTNFTDHDGLHVGIMGAYGYQDSKT
                     HNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGLYMDAWLQYGWFNNTVKGDGLTGEK
                     YSSKGITGALEAGYIYPTIRWTAHNNIDNALYLNPQVQITRHGVKANDYIEHNGTMVT
                     SSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPFLEVNWKWSSKQYGVIMNGMSNHQI
                     GNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNSYRDTQGILGVKYTF"
     gene            3383..3701
                     /locus_tag="ACHUPB_004140"
                     /pseudo
     CDS             3383..3701
                     /locus_tag="ACHUPB_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1157 a          660 c          916 g         1091 t
ORIGIN      
        1 cggtaaagga aaaaacatgc aaattgtctt tttctccttt tgttgttggt gcatccctgt
       61 tgctcggggg gccaatagct tttgctattc ctctttcggg tactcaagaa cttcattttt
      121 cagaggacaa ttatgaaaaa ttattaacac ctgttgatgg actttctccc ttgggagctg
      181 gtgaagatgg aatggatgcg tggtatataa cttcttccaa cccctctcat gcatctagaa
      241 ctaagctacg gattaactct gatattatga ttagcgcagg tcatggtggt gctggtgata
      301 ataatgatgg taatagttgt ggcggtaatg gtggtgactc tattaccgga tctgacttgt
      361 ctataatcaa tcaaggcatg attcttggtg gtaacggcgg tagcggtgct gaccataacg
      421 gtgatggtgg tgaggctgtt acaggagaca atctgtttat aataaatgga gaaattattt
      481 caggtggaca tggtggcgat agttatagtg atagtgatgg ggggaatgga ggtgatgccg
      541 tcacaggagt caatctaccc ataatcaaca aagggactat ttccggtggt aatggaggta
      601 acaattatgg tgagggtgat ggcggtaatg gaggtgatgc catcacagga agcagcctct
      661 ctgtaatcaa taagggcacg ttcgctggag gcaacggagg tgctgcttac ggttatggtt
      721 atgatggcta cggtggtaat gctatcacag gagataacct gtctataatc aacaatggag
      781 ctattttagg cggtaatggt ggacattggg gggatgctat aaatggtagc aatatgacca
      841 ttgctaatag cggatatata atttcaggta aagaagatga tggaacacaa aatgtagtag
      901 gtaatgctat ccacatcact ggtggaaaca attcattaat actccatgaa ggttctgtca
      961 ttactggtga tgtacaggtt aacaattcat ccattctgaa aattatcaac aatgattaca
     1021 ctgggaccac accaactatt gaaggtgatt tatgtgctgg tgattgtaca actgtttcac
     1081 tatcaggtaa caaattcact gtttcaggtg acgtttcttt tggtgagaac agttctttaa
     1141 atttagctgg aatcagtagt ctggaagctt ctggaaatat gtcatttggc aacaatgtaa
     1201 aagtggaagc tattataaat aactgggcgc agaaggacta taaactgcta agtgcagata
     1261 aagggataac aggtttcagt gtttctaata tatctatcat caatccgtta ctcactactg
     1321 gtgctattga ctatacaaaa agctatatca gtgaccagaa taaattgatc tacggtttga
     1381 gctggaatga tacagatggc gacagtcatg gagagttcaa tctgaaagaa aacgctgaac
     1441 ttactgttag tactattctg gcagataatc tcagccatca taatataaat agctgggacg
     1501 gaaaatccct aacaaaatca ggggagggaa ctctcatttt ggcggaaaaa aatacctact
     1561 ctggtttcac caacatcaat gcaggcattc taaaaatggg gacagttgaa gctatgacac
     1621 gtaccgctgg tgttattgtt aataaaggtg ctaccttgaa tttttcaggc atgaaccaaa
     1681 ctgttaacac tttattaaat agtgggactg tgctaatcaa taatattaat gccccttttt
     1741 tgcctgaccc cgtcattgtc acaggtaaca tgactctgga gaaaaacggt catgttattc
     1801 tcaataatag ttcgtcaaat gtcggtcaga cctatgttca gaaaggtaat tggcatggaa
     1861 agggcggaat attatctttg ggcgcggttc tcggcaatga caacagtaaa actgaccggc
     1921 tggaaattgc aggccatgcg tctggtatta cctatgttgc agtgacaaat gagggaggct
     1981 ctggagataa aactcttgaa ggtgttcaaa ttatttcgac agattcttct gataagaatg
     2041 cttttattca gaaaggccgt attgttgctg gtagttatga ctatcgcctg aaacagggca
     2101 ctgtatctgg actgaatacc aataagtggt atctaactag tcagatggat aatcaagaat
     2161 caaaacagat gagcaatcaa gagtctactc aaatgagtag tcgccgagct agttcacagc
     2221 ttgtatcttc acttaatttg ggtgaaggta gtattcacac atggcgccct gaagctggca
     2281 gttatattgc taacctgata gcaatgaaca cgatgtttag tccttctctc tatgaccgac
     2341 acggtagcac tattgttgat cctactacag gtcagctcag cgaaaccacc atgtggattc
     2401 gtactgttgg tggacataat gagcataatt tagctgatag acaattaaaa accacagcta
     2461 acaggatggt ttatcagatt ggtggagata ttttgaagac aaacttcact gatcatgatg
     2521 gcttgcatgt gggtattatg ggagcttatg gatatcagga tagcaaaact cataataagt
     2581 atactagtta tagttcacga ggaactgtga gcggttatac tgccggtttg tacagttctt
     2641 ggtttcagaa tgaaaaagaa cgaacaggtc tatatatgga tgcttggttg cagtacggtt
     2701 ggtttaataa tacagtcaaa ggagatgggt taactggtga gaaatattcc agcaaaggaa
     2761 taacaggagc tttggaagct ggctatatct acccaaccat acgctggact gctcataata
     2821 atattgacaa cgcattgtat ctcaatccac aagtccagat aactaggcat ggggtaaaag
     2881 caaacgacta tattgaacac aatggcacta tggtcacatc ctctggggtc aataatattc
     2941 aagcaaaatt gggattgcgt acatccttaa ttagtcagag ttgtatcgat aaggagactc
     3001 ttcgtaagtt cgaaccattt ttggaagtga attggaaatg gagctcaaag caatatggtg
     3061 taattatgaa tggcatgtca aatcaccaga taggcaaccg taatgtgatt gaactcaaaa
     3121 ctggtgtggg ggggcgtctt gcagataacc taagcatctg gggaaacgta tctcagcaat
     3181 tgggtaataa cagttacaga gacacccaag gtattttggg tgtgaaatat accttctgat
     3241 aataaaaatg cagggcgtgt tgatgtcctg catttctctg ggacccaccg tgtaaaagct
     3301 cggtgggttt ttgtgcacat gatggtatgc ctgcgatcaa tgccaaacgt gtttaccaga
     3361 tcatgcgcca gaatgcgctg ttgcttggcg ttacacagga attgcattag aaagcgatga
     3421 acgaatgcta ccgccaaata ttcgtggtta tgcgcctcag attactggta ttgcagaaac
     3481 taatgcacgt gttgttgtat ctcaacaagg tcgagtaata tatgactcta ctgtaccagc
     3541 tggaactttt tctattcagg acttgagtag ttctgtacga ggaatattgg atgttgaaat
     3601 ttttgagcaa aatggtaaaa gaaaacattt ccaagtagaa atgtgcaggt gtgcctttct
     3661 tatccagact tggtcagagt aggtataaat tagtaacagg tctacctaaa acaaataata
     3721 aagcaactgg tgatgcattt ttttcggtga agatcctacg gggtcctgag cccggtgaag
     3781 tggcgaggca aattacatgg ggaagcgtac agcctgaact gaac
//
LOCUS       DAZOZD010000323         3811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOZD010000323 DAZOZD010000000
VERSION     DAZOZD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.327"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..172)
                     /gene="yneK"
                     /locus_tag="ACHUPB_004141"
     CDS             complement(<1..172)
                     /gene="yneK"
                     /locus_tag="ACHUPB_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0760794.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATK"
     gene            complement(249..1130)
                     /locus_tag="ACHUPB_004142"
     CDS             complement(249..1130)
                     /locus_tag="ACHUPB_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0760795.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            1231..2619
                     /gene="sad"
                     /locus_tag="ACHUPB_004143"
     CDS             1231..2619
                     /gene="sad"
                     /locus_tag="ACHUPB_004143"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR0760796.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2683..3609
                     /gene="glsB"
                     /locus_tag="ACHUPB_004144"
     CDS             2683..3609
                     /gene="glsB"
                     /locus_tag="ACHUPB_004144"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0760797.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3609..>3811
                     /locus_tag="ACHUPB_004145"
     CDS             3609..>3811
                     /locus_tag="ACHUPB_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR0760798.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
BASE COUNT          928 a          891 c         1042 g          950 t
ORIGIN      
        1 tttttgtggc tgagttgatg tttaaagacg tgcgccactc ttcatcacat attattgtac
       61 ttagcgcatc aataaagtcc tttaaaactt cttttacctg tggtgcaata ttacgaacgg
      121 gaaaatcatc aggaagagat atgtaattgc tgatgcttac tggcgtaacc atatttgccc
      181 catatagtca taagtcatcg gaacatcact atatagagca tattagccgc aagaattatc
      241 aaaatagttc attggtaact ctgttttgcc gagtcagaca catccagtag ttgaataaat
      301 gcctcaagcg gacgcgtttt cgcaccgcga cgccagacca gccaggttgt taaccaacgc
      361 cattgctcag ctaacggcca cgcttcaacc tggtgatgcc ccggcatact ttccagcata
      421 gagcgtggaa taagcgcaat gcctgctcct gcgaccacac aggccaacat cccgtgatac
      481 gactccatct cgtgaatagt tcccggagcg gcaccgtcag catgaaacca gctctcgaag
      541 tggcgacgat acgaacaatt ggtgcggaag gcataaatgt tactgccgtt aacctgactg
      601 gcgcggatta ctggcgcatg accttgtggc gtgacgatca tcagctcctc gcggtatacc
      661 ggtatcccgt caatggcagt atggttaatg ggcccatcaa taaatgccgc attcagtttt
      721 ccctccagca ctccttccag catagtgccg gaagggccgg tggaaagtga aaactgaatt
      781 ttgggataac gatggttgaa ttccgccagc gtggccggaa tgcgcactgc tgcggtgctt
      841 tccagcgaac caagagaaaa taaaccttgc ggctcatcgc cagcgacaac gctccgcgct
      901 tcatccacta acgcgagaat ttgttggcta tagcgtaaaa agttatgccc ggcaggagag
      961 agacgtaaac gctgattctc gcgaataaac agatctaccc ccagttctgt ttccagctgg
     1021 cgtagacggg tagtgagatt cgacggtacg cgatgcactt ttgctgcagc ctgggtaatg
     1081 ctgccagctt cggcaaccgc gttgaacatc tccagttggg tcagatccat aacattctcg
     1141 attcgtgaat aagtggctta atattattca ttttaaagca agagtaaatc tgcgtatctt
     1201 cataccatga ctcataaagg agataccccg atgaccatta ctccggcaac tcatgcaatt
     1261 tcaataaatc ctgccacggg tgaacaactt tctgtgctgc cgtgggctgg cgctaacgat
     1321 atcgaaaacg cacttgagct ggcggcagca ggctttcgcg actggcgcga gacaaatata
     1381 gattatcgtg ctgaaaaaat gcgtgatatc ggtaaggctc tgcgcgcccg tagcgaagaa
     1441 atggcgcaaa tgatcacccg cgaaatgggc aaaccaatca accaggcgcg cgctgaagtg
     1501 gcgaaatcgg cgaatttgtg tgactggtat gcagaacatg gtccggcaat gctgaaggcg
     1561 gaacctacac tggtggaaaa tcagcaggcg gttattgagt atcgaccgtt ggggacgatt
     1621 ctggcgatta tgccgtggaa ttttccgtta tggcaggtga tgcgtggcgc tgttcccatc
     1681 attcttgcag gtaacggcta cttacttaaa catgcgccga atgtgatggg atgtgctcag
     1741 ctcattgccc aggtgtttaa agatgcggga atcccgcaag gcgtatatgg ctggctgaat
     1801 gccgacaacg acggtgtcag tcagatgatt aaagactcgc gcattgctgc tgtcacggtg
     1861 accggaagtg ttcgtgctgg agcggctatt ggcgcacagg ctggagcggc actgaaaaaa
     1921 tgcgtactgg aactgggcgg ttcggatccg tttattgtgc ttaacgatgc cgatctggaa
     1981 ctggcggtta aagcggcggt agccggacgt tatcagaata ccggacaggt atgtgcagcg
     2041 gcaaaacgct ttattatcga agagggaatt gcttctgcat ttaccgaacg ttttgtggca
     2101 gctgcggcag ccttgaaaat gggcgatccc cgtgacgaag agaacactct cggaccaatg
     2161 gctcgttttg atttacgtga tgagctgcat catcaggtag agaaaaccct ggcgcagggt
     2221 gcgcgtttgt tactgggcgg ggaaaagatg gctggggcag gtaactacta tccgccaacg
     2281 gttctggcga atgttacccc agaaatgacc gcgtttcggg aagaaatgtt tggccccgtt
     2341 gcggcaatca ccgttgcgaa agatgcagaa catgcgctgg aactggctaa tgatagtgag
     2401 ttcggccttt cagcgaccat ttttaccacc gacgaaacac aggccagaca gatggcggca
     2461 cgtctggaat gcggtggggt gtttatcaat ggttattgtg ccagcgacgc gcgagtggcc
     2521 tttggtggcg tgaaaaagag tggctttggt cgtgagcttt cccatttcgg cttacacgaa
     2581 ttctgtaata tccagacggt gtggaaagac cggatctgac gcgacatatt cagctctgat
     2641 atactcgcag gtcttttcag acctgcggtc caggagtaga aagtggcagt cgccatggat
     2701 aatgcaattt tagaaaacat cttgcggcaa gtgcggccgc tcattggtca gggtaaagtc
     2761 gcggattata ttccggcgct ggctacagta gacggttccc gattgggcat tgctatctgt
     2821 accgttgacg gacagctttt tcaggccgga gacgcgcaag aacgtttttc cattcagtct
     2881 atttccaaag tgctgagtct cgttgtcgcc atgcgtcatt actccgaaga ggaaatctgg
     2941 caacgcgtcg gcaaagatcc gtctggatca ccgttcaatt ctttagtgca actggaaatg
     3001 gaacagggta ttccgcgtaa tccgtttatt aatgctggtg cgctggtggt ctgcgatatg
     3061 ttgcaagggc gattaagcgc accacggcaa cgtatgctgg aagtcgtgcg cggcttaagc
     3121 ggtgtgtctg atatttcata cgatacggtg gtagcgcgtt ccgaatttga acattccgcg
     3181 cgaaatgcgg ctatcgcctg gctgatgaag tcgtttggca atttccatca tgacgtgaca
     3241 accgttctgc aaaactactt tcattactgc gctctgaaaa tgagctgtgt agagctggct
     3301 cggacgtttg tctttctggc taatcagggg aaagctattc atattgatga acctgtagtg
     3361 acgccaatgc aggcgcggca aattaacgcg ctgatggcga ccagtggtat gtaccagaac
     3421 gcgggggagt ttgcctggcg ggtgggacta ccggcgaaat ctggcgttgg tggcggtatt
     3481 gtggcgattg ttccgcatga aatggccatc gcagtctgga gtccggaact ggatgatgca
     3541 ggtaactcgc ttgcgggtat cgccgtcctt gaacaattga cgaaacagtt aggacgttcg
     3601 gtttattaat gcagtctctc gatccactct tcgcgcgttt atcccgttca aaatttcgct
     3661 ctcgctttcg tctgggcatg aaagagcgtc agtattgcct ggagaaaggc gcgccagtca
     3721 tcgaacaaca tgcggcggat tttgtcgcta aacgtcttgc tccggcaggt aatgactcca
     3781 acttattgat agtgttttat gttcagataa t
//
LOCUS       DAZOZD010000324         3799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOZD010000324 DAZOZD010000000
VERSION     DAZOZD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.328"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHUPB_004146"
     CDS             <1..83
                     /locus_tag="ACHUPB_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760799.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(100..909)
                     /gene="proC"
                     /locus_tag="ACHUPB_004147"
     CDS             complement(100..909)
                     /gene="proC"
                     /locus_tag="ACHUPB_004147"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0760800.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1029..1487
                     /locus_tag="ACHUPB_004148"
     CDS             1029..1487
                     /locus_tag="ACHUPB_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0760801.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1670..2194
                     /gene="aroL"
                     /locus_tag="ACHUPB_004149"
     CDS             1670..2194
                     /gene="aroL"
                     /locus_tag="ACHUPB_004149"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0760802.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2244..2435
                     /gene="yaiA"
                     /locus_tag="ACHUPB_004150"
     CDS             2244..2435
                     /gene="yaiA"
                     /locus_tag="ACHUPB_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0760803.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2693..3370
                     /locus_tag="ACHUPB_004151"
     CDS             2693..3370
                     /locus_tag="ACHUPB_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0760804.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3442..3726
                     /gene="ppnP"
                     /locus_tag="ACHUPB_004152"
     CDS             3442..3726
                     /gene="ppnP"
                     /locus_tag="ACHUPB_004152"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0760805.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          952 a          904 c          990 g          953 t
ORIGIN      
        1 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
       61 accgcaccga agtggcggcc tgacgtccgg cgaaagtcat caggatttgc tgagtttttc
      121 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
      181 cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat ctttcagtgc
      241 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
      301 aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
      361 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgaccaccg ggtggatcat
      421 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
      481 atcttcgggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
      541 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gggcaagctg
      601 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
      661 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcggcaaaaa tgatgtcggc
      721 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
      781 ggcgacttta tccggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
      841 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
      901 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
      961 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
     1021 atgactttat gacaatttgg gtggatgccg acgcgtgtcc caatgtaatt aaagagattt
     1081 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
     1141 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
     1201 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
     1261 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
     1321 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
     1381 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
     1441 cggagctgga gaagtggtgg ctggaagtgc aacgtagtcg tggctaaatg taatttatta
     1501 tttacacttc attcttgaat atttattggt atagtaaggg atgtattgag attttcactt
     1561 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggtatg atcgctattc
     1621 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
     1681 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
     1741 ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc tcaatatgac
     1801 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag aaacggcggc
     1861 gctggaagcg gtaactgcgc catccaccgt tatcgctaca ggcggcggca ttattctgac
     1921 ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
     1981 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
     2041 gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctatatcg
     2101 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
     2161 tcgtagcgcc ctggcacaga cgatcaattg ttgattttag agcgcctata cttaacgttc
     2221 atcccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
     2281 tagtgacgat tgaaaaagga aagccaggac agacggtaac ctggtaccaa ctcagagccg
     2341 atcatcctaa accagactcg ttgatcagtg aacatccgac ggctcaggaa gcgatggatg
     2401 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
     2461 tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt tttctaataa
     2521 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
     2581 gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc agcagtggtg
     2641 ctccacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa caatgagtgc
     2701 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaggttttgc cgctcctgac
     2761 ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagtcgtga
     2821 agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca cattattaaa
     2881 tgacaaccag ctggcccatg tttcgcgtcg taaagtggag cgagacctgc aaggtgtggt
     2941 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
     3001 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccactggt
     3061 ttcctctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agatgctgac
     3121 cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat tgggtaaccc
     3181 cattcatgat tcagaacaaa aaatcattga tgccgggaaa gaattactgg caaaaggggc
     3241 tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt tactgcaaaa
     3301 acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg ctgcggaatt
     3361 actggtgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
     3421 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
     3481 tcaatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggc
     3541 gaatacacct tcagcaccgc tgagccggaa gagatgacgg taatcagtgg cgcgctgaat
     3601 gtgttactgc ctgacgcgac cgactggcag gtgtatgaag ccggttcggt gtttaatgtt
     3661 cccggtcaca gtgagtttca tctgcaagtt gccgaaccca cctcttatct gtgccgctat
     3721 ctgtaattcc tcgccttccc cttgaacggg agggcatttt tctgaaatat cctctcttta
     3781 acccataata atatttcct
//
LOCUS       DAZOZD010000325         3777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOZD010000325 DAZOZD010000000
VERSION     DAZOZD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.329"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            231..2147
                     /gene="dnaK"
                     /locus_tag="ACHUPB_004153"
     CDS             231..2147
                     /gene="dnaK"
                     /locus_tag="ACHUPB_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0760806.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2236..3366
                     /gene="dnaJ"
                     /locus_tag="ACHUPB_004154"
     CDS             2236..3366
                     /gene="dnaJ"
                     /locus_tag="ACHUPB_004154"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0760807.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3470..3622)
                     /gene="mokC"
                     /locus_tag="ACHUPB_004155"
     CDS             complement(3470..3622)
                     /gene="mokC"
                     /locus_tag="ACHUPB_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0760808.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         1000 a          939 c         1024 g          814 t
ORIGIN      
        1 aattgctgtc aattttacgt cttgtcctgc catatcgcga aatttctgcg caaaagcaca
       61 aaaaattttt gcatctcccc cttgatgacg tgggttacga ccccatttag tagtcaaccg
      121 cagtgagtga gtctgcaaaa aaatgaaatt gggcagttga aaccagacgt ttcgccccta
      181 ttacagactc acaaccacat gatgaccgaa tatatagtgg agacgtttag atgggtaaaa
      241 taattggtat cgacctgggt actaccaact cttgtgtagc gattatggat ggcaccactc
      301 ctcgcgtgct ggagaacgcc gaaggcgatc gcaccacgcc ttctattatt gcctataccc
      361 aggatggtga aactctggtt ggtcagccgg ctaaacgtca ggcagtgacg aacccgcaaa
      421 acaccctgtt tgcgattaaa cgcctgattg gccgccgctt ccaggacgaa gaagtacagc
      481 gtgatgtttc catcatgccg ttcaaaatta ttgctgctga taacggcgac gcatgggtcg
      541 aagttaaagg ccagaaaatg gcaccgccgc agatttctgc tgaagtgctg aaaaaaatga
      601 agaaaaccgc tgaagattac ttgggtgaac cggtaactga agctgttatc accgtaccgg
      661 catactttaa cgatgctcag cgtcaggcaa ccaaagacgc aggccgtatc gctggtctgg
      721 aagtaaaacg tatcatcaac gaaccgaccg cagctgcgct ggcttacggt ctggacaaag
      781 gtactggcaa ccgtactatc gcggtttatg acctgggtgg tggtactttc gatatttcta
      841 ttatcgaaat cgacgaagtt gacggcgaaa aaaccttcga agttctggct accaacggtg
      901 atacccacct ggggggtgaa gacttcgaca gccgtctgat caactacctg gttgaagaat
      961 tcaagaaaga tcagggcatt gacctgcgca acgatccgct ggcaatgcag cgcctgaaag
     1021 aagcggcaga aaaagcgaaa atcgaactgt cttccgctca gcagaccgac gttaacctgc
     1081 cgtacatcac tgcagatgcg accggtccga aacacatgaa catcaaagtg actcgtgcga
     1141 aactggaaag cctggttgaa gatctggtaa accgttccat tgagccgctg aaagttgcac
     1201 tgcaggacgc tggcctgtcc gtatctgata tcgacgacgt tatcctcgtg ggtggtcaga
     1261 ctcgtatgcc aatggttcag aagaaagttg ctgagttctt tggtaaagag ccgcgtaaag
     1321 acgttaaccc ggacgaagct gtagcaatcg gtgctgctgt tcagggtggt gttctgactg
     1381 gtgacgtaaa agacgtactg ctgctggacg ttaccccgct gtctctgggt atcgaaacca
     1441 tgggcggtgt gatgacgacg ctgatcgcga aaaataccac tatcccgacc aagcacagcc
     1501 aggtgttctc taccgctgaa gacaaccagt ctgcggtaac catccatgtg ctgcagggtg
     1561 aacgtaaacg tgcagctgat aacaaatctc tgggtcagtt caacctggat ggtatcaacc
     1621 cggcaccgcg cggcatgccg cagatcgaag ttaccttcga tatcgatgct gacggtatct
     1681 tgcacgtttc cgcgaaagat aaaaacagcg gtaaagagca gaagatcacc atcaaggctt
     1741 cttctggtct gaacgaagat gaaatccaga aaatggtacg cgacgcagaa gctaacgccg
     1801 aagctgaccg taagtttgaa gagctggtac agactcgcaa ccagggcgac catctgctgc
     1861 acagcacccg taagcaggtt gaagaagcag gcgacaaact gccggctgac gacaaaactg
     1921 ctatcgagtc tgcgctgact gcactggaaa ctgctctgaa aggtgaagac aaagccgcta
     1981 tcgaagcgaa aatgcaggaa ctggcacagg tttcccagaa actgatggaa atcgcccagc
     2041 agcaacatgc ccagcagcag actgccggtg ctgatgcttc tgcaaacaac gcgaaagatg
     2101 acgatgttgt cgacgctgaa tttgaagaag tcaaagacaa aaaataatcg ccctataaac
     2161 gggtaattat actgacacgg gcgaagggga atttcctctc cgcccgtgca ttcatctagg
     2221 ggcaatttaa aaaagatggc taagcaagat tattacgaga ttttaggcgt ttccaaaaca
     2281 gcggaagagc gtgaaatcaa aaaggcctac aaacgcctgg ccatgaaata ccacccggac
     2341 cgtaaccagg gtgacaaaga ggccgaggcg aaatttaaag agatcaagga agcttatgaa
     2401 gttctgaccg actcgcaaaa acgtgcggca tacgatcagt atggtcatgc tgcgtttgag
     2461 caaggtggca tgggcggcgg cggttttggc ggcggcgcag acttcagcga tatttttggt
     2521 gacgttttcg gcgatatttt tggcggcgga cgtggtcgtc aacgtgcggc gcgcggtgct
     2581 gatttacgct ataacatgga gctcaccctc gaagaagctg tacgtggcgt gaccaaagag
     2641 atccgcattc cgactctgga agagtgtgac gtttgccacg gtagcggtgc aaaaccaggt
     2701 acacagccgc agacctgtcc gacctgtcat ggttctggtc aggtgcagat gcgccaggga
     2761 tttttcgctg tacagcagac ctgtccacac tgtcagggcc gcggtacgct gatcaaagat
     2821 ccgtgcaaca aatgtcatgg tcatggtcgt gttgagcgca gcaaaacgct gtccgttaaa
     2881 atcccggcag gggtggacac tggagaccgc atccgtcttg cgggcgaagg tgaagcgggc
     2941 gagcatggcg caccggcagg cgatctgtac gttcaggttc aggttaaaca gcacccgatt
     3001 ttcgagcgtg aaggcaacaa cctctattgc gaagtcccga tcaacttcgc tatggcggcg
     3061 ctgggtggcg aaatcgaagt accgaccctt gatggtcgcg tcaaactgaa agtgcctggc
     3121 gaaacccaga ccggtaagct tttccgtatg cgcggtaaag gcgtcaagtc tgtccgcggt
     3181 ggcgcacagg gtgatttgct gtgccgcgtt gtcgtcgaaa caccggtagg cctgaacgag
     3241 aagcagaaac agttgctgca agagctgcaa gaaagcttcg gtggcccaac cggtgagcac
     3301 aacagcccgc gctcaaagag cttctttgat ggtgtgaaga agttttttga cgacctgacc
     3361 cgctaacctc cccaaaagcc tgcccgtggg caggcttggg taaaattagg gtgcgttgaa
     3421 gatatgcgag cacctgaaaa gtggcgggga tcactccccg ccgttgctct tactcggatt
     3481 cgtaagccgt gaaaacagca acctccgtct ggccagttcg gatgtgaacc tcacagaggt
     3541 cttttctcgt taccagcgcc gccactacgg cggtgataca gatgacgatc agggcgacaa
     3601 tcatcgcctt atgctgcttc attgctctct tctccttgac cttacggtca gtaagaggca
     3661 ctctacatgt gttcagcata tagggggcct cgggttgata gtaaaatatc actcggggct
     3721 tttctctatc tgccgttcag ctaatgcctg agacagatag cctcaagcac ccgccgc
//
LOCUS       DAZOZD010000326         3774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOZD010000326 DAZOZD010000000
VERSION     DAZOZD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.330"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..637)
                     /locus_tag="ACHUPB_004156"
     CDS             complement(<1..637)
                     /locus_tag="ACHUPB_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFR0760809.1"
                     /translation="MTVAIALVNGKGGVKKSSMSRAIATEYTKADWKVLGLDMDVDQG
                     SFLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVIDSAAYATKSLLSLAE
                     IVDLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVSESESEYRTALEYLK
                     PCGVQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQGVIDLLTK"
     gene            1003..1353
                     /locus_tag="ACHUPB_004157"
     CDS             1003..1353
                     /locus_tag="ACHUPB_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0760810.1"
                     /translation="MWTVYFGRLFDEWFEEQELALKRKVLAELKHLEEFGPSLSRPHA
                     DTVKGSRHKNMKELRIQYEGHPIRAFFAFDPIRQAIILCAGDKSNDKKFYDRMIHIAD
                     EEFSTHLAELEGKK"
     gene            1350..1649
                     /locus_tag="ACHUPB_004158"
     CDS             1350..1649
                     /locus_tag="ACHUPB_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0760811.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADEMILETGLQMMREELQLSQK
                     QVADAMGISQPAVTQLEQRGNDLKLATLKRYVEAMGGKLSLDVELPTGKRVAFNI"
     gene            complement(1680..1925)
                     /locus_tag="ACHUPB_004159"
     CDS             complement(1680..1925)
                     /locus_tag="ACHUPB_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760812.1"
                     /translation="MQTKPTPADVKEARISAGLTLKEAAEIFGYQLNTWQMKESAGKA
                     TRSLSVGEYNYLLLLAGKHPEYVLCKRDTITAEPTTV"
     gene            complement(1915..2172)
                     /locus_tag="ACHUPB_004160"
     CDS             complement(1915..2172)
                     /locus_tag="ACHUPB_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760813.1"
                     /translation="MKTDSVFFKSKGSYSYVVTVDMDNQMYMVRKYVKTYVKSCDKEQ
                     VKGKKTVKKLVLDTNLTLPELLDLDNGILADLFTRGNNNAN"
     gene            complement(2208..2750)
                     /locus_tag="ACHUPB_004161"
     CDS             complement(2208..2750)
                     /locus_tag="ACHUPB_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760814.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTELRK
                     YSIIIRLERERFFIVGSVDSLVWHGNPSVCTVSLTRNPVGIANDISRKILANALDEIE
                     LLRKHENALQKKQDKVQIIKGMLSQLISISPWYGALTGFNVDNGLTGKILQNGDDYEM
                     VIRGLNVDQLIKVAGFIKKL"
     gene            complement(2878..3411)
                     /locus_tag="ACHUPB_004162"
     CDS             complement(2878..3411)
                     /locus_tag="ACHUPB_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0760815.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKDAFKKAALKYHPDRNPVGAEMM
                     KAVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKNLYGLSGLVYEVIGNWIW
                     ISGNTIEHKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQ
                     AGGWQQVEGRARVEARA"
BASE COUNT         1120 a          840 c          669 g         1145 t
ORIGIN      
        1 gtttagtcag caagtcaata actccctgga caacttcgtc agctttctgc cgtggacctg
       61 gaaaagggga ttccgtcaat gccagtcctt tatcctgtgc ctggctgaac gaggtcataa
      121 aaggtattgc gccattgata acttgaacac cacacggttt aagatattca agcgcggtac
      181 gatactcact ttcgctttca ctgactccga caaaaactac agccatctta tttgttgagt
      241 gtcccttgtt acgcaatgca ttcacggttg taaccgtcgt tctcaaatcg tccacactaa
      301 aaccagacgg taacacgagc aaatcaacaa tttctgccag gctcaacagc gatttagttg
      361 cataagcagc actatcgata acgactaaat cccatatccc tgattcaata tgttttttag
      421 cctgtgatac cgttccgcat ccctgaatag tgacaggtgg ttcaacttta tatctcaatc
      481 gccgctgtcc ccaatccagg aatgacccct gatcaacatc catatcaagc cctaatacct
      541 tccagtctgc cttcgtgtac tctgtggcaa tagccctgct cattgaggac tttttcaccc
      601 cgcctttacc gttaaccagc gcaatagcta ccgtcatacg accccctata aaaatatttt
      661 tatagaaaaa tataattata gtatccgtat tggtgccaag aattgcaaca actatttgaa
      721 ctacaggcgg caagtgaaga gataacttgt gaatataagg ctttcacaac caaattcagc
      781 caaaacaaaa aatgacgttt tattaacaaa tacgcaacat aaaaaacggt ggaaaacgcc
      841 caggaaaaca tgatcttttg agcggatttt gcagatccga caacgaacgg tgatccgatg
      901 gtgccgataa cgtccattat gttaaatagc cccgtttccc ttgaaattat aagtcgtagc
      961 ttatacaatc atctataagc tataacttat aggaaaaggg acatgtggac tgtttatttt
     1021 ggcagattat ttgatgaatg gtttgaagag caggaactag ccctaaaacg aaaagtgctg
     1081 gctgaactta aacatcttga agagtttggc ccctctcttt cacggccaca tgcagataca
     1141 gtgaaagggt cacgtcataa gaatatgaag gaactacgta tacaatatga aggacatcca
     1201 atcagggcat tctttgcatt tgatccaatt agacaagcaa tcattctttg cgctggcgac
     1261 aaaagtaatg ataagaaatt ttacgacaga atgattcata ttgctgacga agaattttcc
     1321 actcacttag ccgaactgga gggcaaaaaa tgagaacatt agaagaagta attgcggctc
     1381 aatcacctga aagtcaggcg atcatcaaag ctatggctga tgagatgatt ctggaaacag
     1441 ggctgcaaat gatgcgtgaa gaattgcagc tttcacaaaa acaggttgct gatgcaatgg
     1501 gaatcagtca gcccgctgta actcaactgg aacagcgcgg aaatgatttg aagctggcaa
     1561 cgttaaagcg ttacgttgaa gctatgggag gaaagttgag cctagatgtt gaactcccta
     1621 ccggaaagcg agtggccttt aacatttagt agaaaagggg cgaaaagccc cttttggggt
     1681 cagacagtgg ttggttctgc ggtgatcgta tcccttttac aaagaacata ttcagggtgt
     1741 tttccagcca acaacaataa gtagttatat tcacctacag ataaagacct tgtagcttta
     1801 cctgcgcttt ctttcatttg ccaggtattt aactgatagc caaaaatttc ggcagcttcc
     1861 ttaagcgtta aacctgcgga aattctcgcc tctttaacat ctgccggagt tggtttagtt
     1921 tgcattgtta ttacctcttg taaataaatc agccagaata ccattatcaa gatccaataa
     1981 ttctggcaat gtaagattcg tatcgagcac caatttttta actgtctttt tccctttaac
     2041 ttgctcttta tcacaagatt tcacatatgt cttaacatac tttctgacca tatacatctg
     2101 attatccata tctactgtaa caacataaga gtaactaccc ttacttttaa aaaaaacact
     2161 atcagttttc ataaacaacc ccatataaaa ttcacatcaa cactttgtta tagttttttt
     2221 ataaaaccag ccactttgat aagttgatca acattaagcc ctctgataac catttcataa
     2281 tcatcaccat tttgtagtat ttttcctgta agcccattat ccacattaaa tcccgttaat
     2341 gccccatacc agggggatat agaaatcaac tgtgataaca tccctttgat tatttgtact
     2401 ttatcttgtt ttttctgtaa tgcgttctca tgcttcctga gcaattcaat ttcatctagt
     2461 gcattagcta ggattttccg agatatatca ttagcaatac caacaggatt gcgtgtaaga
     2521 gataccgtac aaacggaagg attaccgtgc catacaagac tatcaacact accaacgata
     2581 aaaaatcttt cacgctccag acgaataatg atggagtatt tacgaagttc tgtagaagtc
     2641 agtttaatac gatgctgata attatccagt ttattaacac gccaaccgtt ccccagaata
     2701 cggcatacaa cttcaaagac aggctgatat ttttctgaaa atgttgtcat gttttcacat
     2761 cctttactgc gctaacatag ccagcaattg cagagtatca gctactcaat ccttttcggg
     2821 ttcgatgccc gaaaggattg ctggcgagga tacagggggg cttacgcccc cgtttcgtta
     2881 tgctctggct tcaactcttg ccctgccttc aacttgttgc catcctccgg cctggaattt
     2941 tccgcttgtg ccatattttg cgcggatttc ctctatcgtg tgctcttcac ggttccagta
     3001 acttttatgt tcttctggac gataaaacca ttgttttttc tttgccgccc atttacaccc
     3061 tatttctttt aatgcttctt tgtgctcgat agtatttcca ctaatccaga tccagttacc
     3121 aataacctca taaactaaac cagacaaacc gtaaagattc tttaaaacat tttctagttc
     3181 atcaccatag ttatatattg cactttgttc aggactttgg tactgattaa tcttatcaag
     3241 attcgccatt aaaaaatcga atgctgaatt aaccgccttc atcatttcag cacctacagg
     3301 attacgatct gggtgatatt ttaatgcagc tttcttaaag gcgtctttga tgtctttttc
     3361 agtcaactga ccagacaagc caaaaatatt taatgcttct tggatattca ttttgtagct
     3421 cccgcccctg tggggctaag atgagaggga aaccatttcc ctctcgatga tttaattata
     3481 ccttcaattg caggtattgc aagtttttta gctctctttt tgaaaaaatt tataattact
     3541 gtcaggacat caacattact taaaacattt catatccctg taaataattt aaccaccacc
     3601 tgaattgatc ccaatccgta aatttcattg ctgggattct cgtttcccat cctttgacaa
     3661 gtgaatccgc aagttgtacg atctgacctg ttgacatttg aacacctctc tattgttttg
     3721 agtgtcgata aagtgatcag gttgctcgac caacctgatc acacctttat agaa
//
LOCUS       DAZOZD010000327         3753 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOZD010000327 DAZOZD010000000
VERSION     DAZOZD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.331"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUPB_004163"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUPB_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0760816.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUPB_004164"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUPB_004164"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR0760817.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUPB_004165"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUPB_004165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR0760818.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          856 a          985 c          910 g         1002 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatcgc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgtg atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgctcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa tta
//
LOCUS       DAZOZD010000328         3750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOZD010000328 DAZOZD010000000
VERSION     DAZOZD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.332"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            78..2357
                     /gene="ydeP"
                     /locus_tag="ACHUPB_004166"
     CDS             78..2357
                     /gene="ydeP"
                     /locus_tag="ACHUPB_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0760819.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2605..2802
                     /gene="safA"
                     /locus_tag="ACHUPB_004167"
     CDS             2605..2802
                     /gene="safA"
                     /locus_tag="ACHUPB_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0760820.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2877..3638
                     /gene="ydeO"
                     /locus_tag="ACHUPB_004168"
     CDS             2877..3638
                     /gene="ydeO"
                     /locus_tag="ACHUPB_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0760821.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT         1047 a          828 c          868 g         1007 t
ORIGIN      
        1 gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa taattcattt
       61 ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct gcaggtggtt
      121 ggggtgctgt taaatccgta gcgaatgcag tacgtaagca gatggatata cgccatgatg
      181 ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt tgtgcatggc
      241 cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa gcaatcgcct
      301 gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg gttcaatcat
      361 tacttacctg gggagatcac gagcttgagg ctgcggggcg tctcactcag cctttgaaat
      421 atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc gacgaaattg
      481 gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact tcgggccgca
      541 cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg agcaataact
      601 ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca gcgagtatcg
      661 gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta gtcatttgca
      721 ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc gctttagtga
      781 aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg gagcgattta
      841 ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg gccagtgcct
      901 actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg cgcctgttaa
      961 ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat gacgaattta
     1021 ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat tccgagtgga
     1081 aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg gctgacgcat
     1141 atgccgctgc cgatcgcacc attatctgtt acggaatggg gatcactcag cacgaacatg
     1201 gtacccagaa tgtacagcaa ctggtcaatc tgctgttgat gaaaggtaac attggcaagc
     1261 ctggtgcggg tatctgccca ctacgaggtc actctaatgt acagggcgac cgaaccgtcg
     1321 gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc tatggcttca
     1381 ccccacctca tgcacctgga catgctgcaa ttgccagcat gcaagcaata tgcacggggc
     1441 aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca gatcgggaag
     1501 cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact aagcttaacc
     1561 gctctcatct gctgaccgca cggcatagct atattctgcc ggttctgggg cgtagcgaga
     1621 ttgacatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg tcgatgattc
     1681 atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag tgtgcagtgg
     1741 tcgcgggaat cgcgcaggca gcactacccc acagcgtggt agcctgggag tatctggtgg
     1801 aagattatga tcgcattcgc aatgacattg aagctgtgtt accagagttc gccgactata
     1861 accagcgcat ccgacatcct ggaggttttc acctgataaa tgcagctgct gaaaggcgct
     1921 ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta gaagatccct
     1981 cttcagcgtt taacagtaag ctggtcatgg cgacagtacg tagccacgat cagtacaaca
     2041 cgacgattta tggtatggat gatcgctatc gaggggtatt cggtcaacga gatgtggtct
     2101 ttatgagtgc taaacaagct aaaatttgcc gtgtaaaaaa cggcgaaaga gttaatctta
     2161 ttgcgcttac gccagacggt aagcgcagct cacgccgcat ggatagatta aaagtggtca
     2221 tttaccctat ggctgaccgc tcactggtga cctattttcc agaatcgaat cacatgctaa
     2281 ctcttgataa ccacgatcca ttaagtggca ttcctggtta taaaagtatt ccggttgaat
     2341 tagaaccatc aaattaattc gcctcctcat ttcttctgct gtcatccgca cagcagaaga
     2401 atttctcatt gactattatc gcgcaatttg ctcacattga ttaaattaaa ccacacacta
     2461 taagatataa acctctgcct acagctgtaa gaaactccgc tcagtactga agtgccagtc
     2521 ctatttcctc ttttctccag cctgttatat taagcatact gattaacgat ttttaacgtt
     2581 atccgctaaa taaacatatt tgaaatgtat gcgaccacag tgaaaaacaa aatcacgcaa
     2641 agagacaact ataaagaaat catgtctgta attgtggttg tcttattact gacccttacg
     2701 ttgatagcca ttttttcggc aattgatcag ctgggtattt cagaaatggg tcgcattgca
     2761 agagatctta cacatttcat tatcaatagt ttgcaagact gaaaataaac agcaaattat
     2821 aaatatgaaa tgttaaaaaa gtatcgataa aaactttatt gttttaagga gataaaatgt
     2881 cgctcgtttg ttctgttata tttattcatc atgccttcaa cgctaacatt ttagataaag
     2941 attacgcctt ctctgacggc gagatcctga tggtagataa cgctgttcgt acgcattttg
     3001 aaccttatga gcggcatttt aaagagatcg gatttactga aaataccatt aaaaaatatc
     3061 tacaatgcac taacatccag acagtgacgg tgcctgttcc tgcgaagttt ttacgtgctt
     3121 caaatgtacc gactggattg cttaatgaaa tgattgctta tcttaactcg gaagaacgca
     3181 atcatcataa tttttcagaa cttttgcttt tttcttgcct gtctattttt gccgcatgca
     3241 aaggtttcat tacactatta actaacggtg tgctatccgt ttccggaaaa gtgagaaata
     3301 ttgtcaacat gaagctggcg cacccatgga agctgaaaga tatttgtgac tgcctgtaca
     3361 tcagtgaaag cctgttgaag aaaaaactaa agcaagagca aacgacattc tcacagattc
     3421 tattagatgc cagaatgcag cacgcaaaaa atttgatacg cgtagaaggt tcagtcaata
     3481 aaattgccga acaatgtggt tatgccagta catcttattt tatttatgcg ttccgcaaac
     3541 atttcggcaa cagtccgaag agagtttcta aggagtaccg ttgtcaaagt cacatgggta
     3601 tgaatacggg caacacgatg agtgctttag ctatttgatt atttgctaac gagtagtcaa
     3661 ccacgcacgc tgcgtaagaa ttaaatgggg cagccattcc ctgccccgcg ttgtttttag
     3721 gcgatatatt tattgaaata aataagtgac
//
LOCUS       DAZOZD010000329         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOZD010000329 DAZOZD010000000
VERSION     DAZOZD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.333"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHUPB_004169"
     CDS             complement(<1..181)
                     /locus_tag="ACHUPB_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR0760822.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHUPB_004170"
     CDS             425..682
                     /locus_tag="ACHUPB_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR0760823.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUPB_004171"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUPB_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0760824.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUPB_004172"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUPB_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0760825.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUPB_004173"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUPB_004173"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0760826.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="ACHUPB_004174"
     CDS             complement(3307..>3734)
                     /locus_tag="ACHUPB_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0760827.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAZOZD010000330         3685 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOZD010000330 DAZOZD010000000
VERSION     DAZOZD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.334"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            159..1262
                     /gene="proB"
                     /locus_tag="ACHUPB_004175"
     CDS             159..1262
                     /gene="proB"
                     /locus_tag="ACHUPB_004175"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0760828.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1274..2527
                     /gene="proA"
                     /locus_tag="ACHUPB_004176"
     CDS             1274..2527
                     /gene="proA"
                     /locus_tag="ACHUPB_004176"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0760829.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2642..2717
                     /locus_tag="ACHUPB_004177"
     tRNA            2642..2717
                     /locus_tag="ACHUPB_004177"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2675..2677,aa:Thr,seq:cgt)
     gene            3323..3481
                     /locus_tag="ACHUPB_004178"
     CDS             3323..3481
                     /locus_tag="ACHUPB_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760830.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          934 a          875 c          986 g          890 t
ORIGIN      
        1 attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc cgcgcaacaa
       61 aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt
      121 ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg
      181 tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata
      241 ttgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg
      301 tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga
      361 ctatcgcctc gaaacaactg ctggcagcgg tagggcagag tcgactgatt caactgtggg
      421 aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc cgtgctgata
      481 tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca
      541 atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaagtcggcg
      601 ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgctgc
      661 tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga
      721 ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtaac agcgtttcag
      781 gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct tgccgtgcgg
      841 gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag
      901 gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct
      961 ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca actgccgcca
     1021 ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct
     1081 cgcgtggtga agtcatccgc atttgcaacc tcgaaggccg cgatatcgcc cacggcgtca
     1141 gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg
     1201 caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt
     1261 aaggagctgg ccgatgctgg aacaaatggg cattgctgcg aagcaagcct cgtataaatt
     1321 agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga
     1381 agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa
     1441 tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat
     1501 tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg
     1561 cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg
     1621 cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac
     1681 cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg ctgcaacggt
     1741 ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg tgcaggcgat
     1801 tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat
     1861 gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt cgacaatccc
     1921 ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg tagagatcgc
     1981 tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat gtaatacggt
     2041 tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca
     2101 aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc agttgcaggc
     2161 aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt ttctgtcatt
     2221 agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca
     2281 cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt
     2341 taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg
     2401 ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc
     2461 aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg
     2521 tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcataaggc cattgacgca
     2581 tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag
     2641 ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac
     2701 tcctattatc ggcaccatta agaaaattaa taacttagct taacttctct aagaaattgt
     2761 atcttctgtt atcacttaca aacgcctata cgttgttatg ggctttggta gtgtaacacg
     2821 tatgggcgag tagttcccag gctcaacctc gtaacaagtt cctttgaagc tggaagacgt
     2881 gccaccgtct ctaaaccttc taccaaaact cttagcaaga tgttccacac ctggcgcggt
     2941 gtccaatgat gctacacgtc gtgagacgtt gcagaggata aacaggctaa atgatgggaa
     3001 gagttttaag tgcaatagtg gattaaagcc cacctcttca aaacctaact tgatcgggaa
     3061 agaatcaagc tctagcacgg tttcgtccac cggagtacaa cgggtgcctg ataagagaag
     3121 aactgctaag acttcatagc ttttgtgaaa aagccctttt tagtggatac gcctacggcg
     3181 cttatttttt ggtataacca tcaaaggcgc tttatggctc ctattgtctt gaaaatgtat
     3241 ctttacttgt attgtgtttt atgcgtagtc tccctgtgta gtatgtaact tgttaatttt
     3301 catatgaata cagaggcaca cgatgaaaaa cattgcagca atcaaacgta ataaccgcaa
     3361 gattcacgct cgtaagttcc tgtctacgcc agaaggaaaa gcctggctag agcgtaaaca
     3421 gagagagaac gaagaaagaa aactccttag tgagttgaaa tggcttagag aggatttctg
     3481 aaaatctcca gaacgcaaca ggacgcattc taacgctgtt ttcattactc ggtataggga
     3541 taggtgatgt gtgtttaatg cgtctgtgcg cttgttttgt gccttatttt gcattttcat
     3601 aatgcggaag agaacacgga aaaccttaaa attaattatt ggggtattta aggctatttt
     3661 caggtgattt aagggtatta aactt
//
LOCUS       DAZOZD010000331         3679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOZD010000331 DAZOZD010000000
VERSION     DAZOZD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.335"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..212
                     /gene="ypdI"
                     /locus_tag="ACHUPB_004179"
     CDS             <1..212
                     /gene="ypdI"
                     /locus_tag="ACHUPB_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR0760831.1"
                     /translation="AFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILFKMREDA
                     PPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(289..531)
                     /locus_tag="ACHUPB_004180"
     CDS             complement(289..531)
                     /locus_tag="ACHUPB_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0760832.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            884..1804
                     /gene="lpxP"
                     /locus_tag="ACHUPB_004181"
     CDS             884..1804
                     /gene="lpxP"
                     /locus_tag="ACHUPB_004181"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0760833.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2160..2231
                     /gene="ypdK"
                     /locus_tag="ACHUPB_004182"
     CDS             2160..2231
                     /gene="ypdK"
                     /locus_tag="ACHUPB_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0760834.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2296..3534)
                     /gene="alaC"
                     /locus_tag="ACHUPB_004183"
     CDS             complement(2296..3534)
                     /gene="alaC"
                     /locus_tag="ACHUPB_004183"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0760835.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT          943 a          947 c          870 g          919 t
ORIGIN      
        1 ttgcattttc catttctggt ggtgtaagtg agaggagcta taaagagact gaaaaaacat
       61 cagcgatgac aaccacacac tctacaaaac ttcagccatc acaggcgatt ttgtttaaga
      121 tgagagaaga tgcgccacca ttaaacctca cagaagaaat cacgcctact tatccgacaa
      181 aggcgaatta tcttattcat cctgtgcgat agcgtctgtc acatggcagg tgaacaatca
      241 tgtctgatgc tgatgtgaac acctgcccaa atgagccatc ctgacccact atcgcctgcc
      301 ataccccacc ataaagcgaa tcatcaagcc acaagccggg gcaagaaaca taaatatccc
      361 cgtcaccagt tcattaccgc cagttaacca catggtgaag taataaatga gtgcagcgag
      421 gatcaccatg ccgaagaaag aagagaccgc cgataccacg ttcagtacct gaccgatgta
      481 gccgcttacg cagacaatac acagggcgag aaacatccat aaataaatca tagctcaacc
      541 tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa tagtaagaca atccttaacc
      601 tctggcaatt tttgctaagc atcaatgaaa acagcacgtt aaatgcaacg ctattgcgtg
      661 cggaaaattt tttactttgc acgattaata atcagtagct gaaagcagtc agccagtgat
      721 aaagtagagc caccgcattc atgctcctcg tggttatgtc ctgactagtc tttacactct
      781 ttacaggaac cattgtcgta catgatggcc caaccaattg agtgtaccgc tgtcgacatg
      841 gaatcttctg cgatacaaca attcgtatct acagaaggta actatgtttc cacaatgcaa
      901 attttcccgc gagtttctac atcctcgcta ctggctcaca tggtttgggc ttggtgtact
      961 ctggctttgg gtacagcttc cttatcctgt tctctgcttt ctcggcacgc gtattggcgc
     1021 aatggcgcga ccattcctga aacgtcgtga atctatcgcc cgtaaaaacc tggaactttg
     1081 tttcccgcag cattctgcgg aagaacgcga gaagatgatt gccgaaaact ttcgttcact
     1141 cggcatggcg ctggtagaaa ccggcatggc atggttctgg cccgacagtc gcgtacgtaa
     1201 atggtttgat gttgaagggt tggataaccc taaacgcgca caaatgcaaa atcgcggcgt
     1261 aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc ggccgcgtga tgggactgtg
     1321 ccaaccaatg atggctacct atcgtccaca taataatcag ctgatggaat gggtgcagac
     1381 ccgtgggcgc atgcgctcta acaaagcgat gatcggcaga aataatctgc gcggcattgt
     1441 cggtgcactg aagaaaggtg aagcggtatg gtttgctccc gatcaggatt atggtcgtaa
     1501 aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc gccacaacca atggcaccta
     1561 tgttctctcc cgtctctctg gcgcagccat gttgaccgta acgatggtaa gaaaagcgga
     1621 ttacagcgga tatcgtttgt ttatcacccc agagatggaa ggctacccga cagatgaaaa
     1681 tcaagccgct gcctatatga acaagattat cgagaaagag atcatgcgcg caccggagca
     1741 gtacctctgg atccaccgtc gctttaaaac gcgcccggtg ggagaatcgt cgttgtacat
     1801 ttaacaaact gctcttactc tttccctcct caaatcggcc ataataacct catggttttt
     1861 atggcttttt tcttaaggtt ttttccttcg ctgaatatct aattattacc gtcagaaatt
     1921 aaactgtcgc cgatatgaga cacttgtaag ttactaaaag taaataagaa aatgcctctt
     1981 gtctcgtttc agttttaggc gtacattaac gccgtcttga gcagtttagc tcagaattct
     2041 ggggtgatgc ccacggtaaa atacagggct tttaaggtcg ctttgcccgg tgtcaactca
     2101 ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa ttacgcggag agatgtaaag
     2161 tgaaatattt ctttatgggc atttctttta tggtcatcgt ttgggccggt acttttgccc
     2221 tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc catcaggctc ctgtttgcat
     2281 ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt ttgctgctgg cgggtagtaa
     2341 accgtcggca cggaacatcg ctttaatccc tctgatggcc tgacgaatac ggtcgcggtt
     2401 ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg ccaaagccaa tccccggcga
     2461 gacacaaacc ttcgcttcgt taaggagctt cttggcaaat tccagcgatc ccatggccgc
     2521 atatggttcc gggattttcg cccagacata catcgaagcc ttcggcattt cgaccatcca
     2581 gcccgcttca tgcagccctt taaccaatac atcacggcgg cgtttgtact gttcggtaat
     2641 gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc gccgccacct gcaacggcgt
     2701 aaaagtgccg taatcgtgat agcttttgat tcgcgccagg gcactgacca gcgttttgtt
     2761 gccgaccata aagccgatac gccagcccgc catgttgtag cttttcgaca gcgtaaagaa
     2821 ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt gacggcgctt tccagccatc
     2881 gtagacgata tcggcatagg ccaggtcatg gaccaccagc acatcgtagc gtttcgccag
     2941 cgccacaacc ttttcgaaga actccagttc cacgcattgc gcggttgggt tcgacgggaa
     3001 gccgaggatc atcatcttcg gtttcggata actttcacga atggcgcgct ccagttcgtt
     3061 gaagaaatcg acaccttcca ccagcggcac tgagcgtacc tgcgccccgg caatcaccgc
     3121 gccataaata tggatcgggt aacttggatt cggcaccagc accgtgtcac catgatccag
     3181 cgtcgccagc atcagatgcg ccaggccctc tttcgaacca atagtgacga tggcttctga
     3241 ttccgggtcg atttcaacgt cgtagcgatc ctgataccag cgggaaatgg cgcgacgtaa
     3301 ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc gggcgctggg ccacagtaca
     3361 taatttttcg acgatatgcg gcggagtcgc accgtccggg ttacccatgc tgaaatcgat
     3421 aatatcttcg ccgcgccgac gcgcagccat tttcagttca gcggtgatgt taaaaacgta
     3481 gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg cgagtgtcag ccataatttc
     3541 ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct gccacaatgg tggctgtttt
     3601 gaaaatagcc tgattaattt ctgtctgtcc agcgttttag aacattttta tccatggctt
     3661 taaatgagga actccactt
//
LOCUS       DAZOZD010000332         3656 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOZD010000332 DAZOZD010000000
VERSION     DAZOZD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.336"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(51..95)
                     /locus_tag="ACHUPB_004184"
     CDS             complement(51..95)
                     /locus_tag="ACHUPB_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760836.1"
                     /translation="MKRNDKILHYEGLN"
     gene            214..1938
                     /gene="ilvI"
                     /locus_tag="ACHUPB_004185"
     CDS             214..1938
                     /gene="ilvI"
                     /locus_tag="ACHUPB_004185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0760837.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1941..2432
                     /gene="ilvN"
                     /locus_tag="ACHUPB_004186"
     CDS             1941..2432
                     /gene="ilvN"
                     /locus_tag="ACHUPB_004186"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0760838.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2444..2530
                     /locus_tag="ACHUPB_004187"
     CDS             2444..2530
                     /locus_tag="ACHUPB_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0760839.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2612..3616
                     /gene="cra"
                     /locus_tag="ACHUPB_004188"
     CDS             2612..3616
                     /gene="cra"
                     /locus_tag="ACHUPB_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0760840.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          859 a          867 c         1011 g          919 t
ORIGIN      
        1 taaaaaaata gagaaattgc tgtaagttgt gggattcagc cgatttatta tcaatttaat
       61 ccttcgtaat ggaggatttt atcgtttctt ttcacctttc ctcctgttta ttcttattac
      121 cctgcgttta tgtctctggc tgccaattgc ttaagcaaga tcggacggtt aatgtgtttt
      181 acacattttt tccgtcaaac agtgaggcag gccatggaga tgttgtctgg agccgagatg
      241 gtcgtccgat cgcttatcga tcagggcgtt aaacaagtat tcggttatcc cggaggcgca
      301 gtccttgata tttacgatgc attgcatacc gtgggtggta tcgatcatgt tttagttcgt
      361 catgagcagg cggcagtgca tatggccgat ggtctggcgc gcgcgaccgg ggaagtcggc
      421 gtcgtgctgg taacgtcggg tccaggggcg accaatgcga ttactggcat cgccaccgct
      481 tatatggatt ccattccatt agttgtcctt tccgggcagg tagcgacctc gttgataggt
      541 tacgatgcct ttcaggagtg cgacatggtg gggatttcgc gaccagtggt taaacacagt
      601 tttctggtta agcaaacgga agacattccg caggtgctga aaaaggcttt ctggctggcg
      661 gcaagtggtc gtcctggacc agtagtcgtt gatttaccga aagatattct taatccggcg
      721 aacaaattac cctatgtctg gccggagtcg gtcagtatgc gttcttacaa tcccactact
      781 accggacata aagggcaaat taagcgcgct ctgcaaacgc tggtagcggc aaaaaaaccg
      841 gttgtctacg taggcggtgg ggcaatcacg gcgggctgcc atcagcagtt gaaagaaacg
      901 gtggaggcgt tgaatctgcc cgttgtttcc tcattgatgg ggctgggagc gtttccggca
      961 acgcatcgtc aggcactggg catgctggga atgcacggta cctacgaagc caatatgacg
     1021 atgcataacg cggatgtgat tttcgccgtc ggggtacgat ttgatgaccg aacgacgaac
     1081 aatctggcaa agtactgccc aaatgccact gttctgcata tcgatattga tcctacttcc
     1141 atttctaaaa ccgtgactgc ggatatcccg attgtggggg atgctcgcca ggtcctcgaa
     1201 caaatgcttg aactcttgtc gcaagaatcc gcccatcaac cactggatga gatccgcgac
     1261 tggtggcagc aaattgaaca gtggcgcgct cgtcagtgcc tgaaatatga cactcacagt
     1321 gaaaagatta aaccgcaggc ggtgatcgag actctttggc ggttgacgaa gggagacgct
     1381 tacgtgacgt ccgatgtcgg gcagcaccag atgtttgctg cactttatta tccattcgac
     1441 aaaccgcgtc gctggatcaa ttccggtggc ctcggcacga tgggttttgg tttacctgcg
     1501 gcactgggcg tcaaaatggc gttgccagaa gaaaccgtgg tttgcgtcac tggcgacggc
     1561 agtattcaga tgaacatcca ggaactgtct accgcgttgc aatacgagtt gcccgtactg
     1621 gtggtgaatc tcaataaccg ctatctgggg atggtgaagc agtggcagga catgatctat
     1681 tccggccgtc attcacaatc ttatatgcaa tcgctacccg atttcgtccg tctggcggaa
     1741 gcctatggtc acgtcgggat ccagatttct catccgcatg agctggaaag caaacttagc
     1801 gaggcgctgg aacaggtgca caataatcgc ctggtgtttg ttgatgttac cgtcgatggc
     1861 agcgagcacg tctacccgat gcagattcgc gggggcggaa tggatgaaat gtggttaagc
     1921 aaaacggaga gaacctgatt atgcgccgga tattatcagt cttactcgaa aatgaatcag
     1981 gcgcgttatc ccgcgtgatt ggcctttttt cccagcgtgg ctacaacatt gaaagcctga
     2041 ccgttgcgtc aaccgatgat ccgacattat cgcgtatgac catccagacc gtgggcgatg
     2101 aaaaagtact tgagcagatc gaaaagcaat tacacaagct ggtcgatgtc ttgcgcgtga
     2161 gtgagttggg gcagggcgcg catgttgagc gggaaatcat gctggtgaaa attcaggcca
     2221 gcggttacgg gcgtgacgaa gtgaaacgta atacggaaat attccgtggg caaattatcg
     2281 atgtcacacc ctcgctttat accgttcaat tagcaggcac cagcgataag cttgatgcat
     2341 ttttagcatc gattcgcgag gtggcgaaaa ttgtggaagt tgctcgttct ggtgtggtcg
     2401 gactttcgcg cggcgataaa ataatgcgtt gagaatgatc tgaatgcgca atttgcagcc
     2461 caacatgtca cgttgggctt tttttgcgaa atcagtggga acctggaata aaagcagttg
     2521 ccgcagttaa ttttatgcgc ttagatgtta atgaatttaa cccataccag tacaatggct
     2581 atggttttta cattttacgc aaggggcaat tgtgaaactg gatgaaatcg ctcggctggc
     2641 gggagtgtcg cggaccactg caagctatgt tattaacggc aaagcgaagc aataccgtgt
     2701 gagcgacaaa accgttgaaa aagtcatggc tgtggtgcgt gagcacaatt accacccgaa
     2761 cgccgtggca gctgggcttc gtgctggacg cacacgttct attggtcttg tgatccccga
     2821 tctggagaac accagctata cccgcatcgc taactatctt gaacgccagg cgcggcaacg
     2881 gggttatcaa ctgctaattg cctgctcaga agatcagcca gacaacgaaa tgcgctgtat
     2941 tgagcacctt ttacagcgtc aggtcgatgc cattattgtt tcgacgtcgt tgcctcctga
     3001 gcatcctttt tatcaacgct gggctaacga cccgttcccg attgtcgcgc tggaccgcgc
     3061 cctcgatcgt gaacacttca ccagcgtggt tggtgccgat caggatgatg ccgaaatgct
     3121 ggcggaagag ttacgtaagt ttcccgccga gacggtgctt tatcttggcg cgctgccgga
     3181 gctttctgtc agcttcctgc gtgaacaagg tttccgtact gcctggaaag atgatccgcg
     3241 cgaagtgcat ttcctgtatg ccaacagcta tgagcgggag gtggctgccc agttattcga
     3301 aaaatggctg gaaacgcatc cgatgccgca ggcgctgttc acaacgtcgt ttgcgttgtt
     3361 gcaaggggtg atggatgtca cgctacgtcg cgatggtaaa ctgccttctg acctggcaat
     3421 tgccaccttt ggcgataacg aactgctcga cttcttacag tgcccggtgc tggcagtggc
     3481 tcaacgtcac cgcgatgtcg cagagcgtgt gctggagatt gtcctggcaa gcctggacga
     3541 accgcgtaag ccaaaacctg gtttaacgcg cattaaacgt aatctctatc gccgcggtgt
     3601 gctcagccgt agctaagccg cgaacaaaaa tacgcgccag gtgaatttcc ctctgg
//
LOCUS       DAZOZD010000333         3628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOZD010000333 DAZOZD010000000
VERSION     DAZOZD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.337"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHUPB_004189"
     tRNA            complement(1..76)
                     /locus_tag="ACHUPB_004189"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHUPB_004190"
     tRNA            complement(79..155)
                     /locus_tag="ACHUPB_004190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHUPB_004191"
     tRNA            complement(157..233)
                     /locus_tag="ACHUPB_004191"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
     gene            complement(423..986)
                     /locus_tag="ACHUPB_004192"
     CDS             complement(423..986)
                     /locus_tag="ACHUPB_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0760841.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1090..1308)
                     /locus_tag="ACHUPB_004193"
     CDS             complement(1090..1308)
                     /locus_tag="ACHUPB_004193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760842.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1467..1853)
                     /locus_tag="ACHUPB_004194"
     CDS             complement(1467..1853)
                     /locus_tag="ACHUPB_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0760843.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1843..2214)
                     /locus_tag="ACHUPB_004195"
     CDS             complement(1843..2214)
                     /locus_tag="ACHUPB_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0760844.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(2214..2471)
                     /locus_tag="ACHUPB_004196"
     CDS             complement(2214..2471)
                     /locus_tag="ACHUPB_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760845.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2468..>3628)
                     /locus_tag="ACHUPB_004197"
     CDS             complement(2468..>3628)
                     /locus_tag="ACHUPB_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0760846.1"
                     /translation="ITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEALQKLRDA
                     HTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEKSRTLMT
                     GIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTMEMANIQI
                     GERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTGRNIWMVDQANLTIDEICAT
                     TKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPLIALSQL
                     SRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQATGIAEINITKQRN
                     GSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYSNTRTDSSKMERFF"
BASE COUNT          846 a         1007 c          812 g          963 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt ttttcaggta taaaaaaacc cgctcatcgg cgggtttaag ctgtgtggcg
      361 tagtaaccac tcttaacagg atattcaact ttttacgatc gtaaagtttt cggaaaaaat
      421 ttttaaaacc gcatcaaccg ctccaccagt tgctctttac gggcaacgat ccacccgtgt
      481 tgctccagat aaaatttaaa ccgttccaga gtacatacca tcgcatcggc gggtaccttt
      541 tccgtgaact caacctgacc gtgtttatcg aagtggctca gtaatgcgca tccatcattt
      601 tcgatagatg tgttttgtgc cgctggtggc tgtttctggt tgaaataaca gtcttccagt
      661 ttttcgaaca cttcccacgc ctgatcggtt tcgagcattt tggcgtgacg ggctgcgcca
      721 cgttctgtcc agaggatgag ggagcgaacg ttacgggcaa ttttcacaga aactcttaaa
      781 gagttttcgt tcttcaactc tcgtaacgca tcaccttcta tcaggaaaaa atgtttacct
      841 tctataaacc gttctttgtt tcgattgaaa ttgtttgtca aacgctggcg ctctgttccg
      901 tacaaatcag ccagcagctc tgttgtcatg acaggaagat ggttatgcgt aagtgacggg
      961 agtttttcaa tagacgtgcg attcatagac atgtcctttc gaatattttg atttacccct
     1021 tttgagaggg tgaccgggcg ctcaaaaccg tcgaaagtcg gcgggcgtat ttccattgct
     1081 ggtattgtat tagccgcaca cccggtcata aaccaagaat tctggacaca aaaaaaccac
     1141 atggctatcg ggtgtggata ccgctttcga ggtgttttga gcaccgcggg gaaatgtaaa
     1201 ctgcaaacct gaaactgtca acaccgtcag gttccaatca tcgggtggtg agacgcacag
     1261 ggttggaact accgggaaac cgaccggcga gcttttcagc tcccccatgc gccccaccat
     1321 aattcagatg tgcgcgtgca tacgacaata aaaaacacgc tcgcggcgtg tgtctgtcgc
     1381 ggtctctatc cggggttcca atcccgacgc cagattttgc tggcgcgtga ggaatatagc
     1441 cccggacaat gtgtctggtc aagcacctac ataattcgtt ctacgtatct gtccatctcc
     1501 agccggatat caagcatcat caacatgcca tcaataaccc cttccgcttt ctgcaggcgc
     1561 ttgccaatac aggtatccga acacccatgt ttccgtgcca gactcataaa agtcattcca
     1621 cctacgtaat aatccaccag caaatcgtgc aaatcctgat ttttcttgtt caaccgggcc
     1681 atacagccac aaattatcat tgcatcatca tcagaacact gagggcgtga tttcactttc
     1741 gacggaatta accctttaaa accagcagcg atcgacgccc aggatacatc ttcatgagtg
     1801 tttgcagccc aagcccccca ccgttccata acctgctgaa tatcacgcgc catcgttatc
     1861 acctgtgatt tcataaatct tcacgcccag tcgcccacca ggaacgagct gaccgcgcac
     1921 aatattgatt tcatcaaact gctcgtcgtc tatgagtagt ccggcatgcg tcagcgcatc
     1981 cagtggtgct ttcaggatat tgtccaggtc acgacggcgc ttatccggtg gctctgcaat
     2041 aatctttatc gccagccttc cggacaggtt taatttcagc cgctgctggc gaacaattag
     2101 cgtcacatca cggcgataac gctcaccaac ttttgataca aaatatgtgc tgccacgacg
     2161 tcgccagtaa gtattcaccg tcggcggata aggtaaaata aactcatggc gcatcagcgc
     2221 agcacctcct gcaccatttt ttcaaacttt ccaactttgg tttccagctc tgccacacaa
     2281 tcaacgagct catccaccgc tttttgtgcg cgatgtttcg cctgcatcag ttccctgagc
     2341 gctggcacca tatcccgacg aatggcatct tttgttacac ctgttttttc cagttgttcc
     2401 gcctgtcgca acatttcctg cgcgtgttta cgcaattgtt cagaggtaaa agtcattgtc
     2461 tggttgttca aaagaaacgc tccatcttac tgctgtcggt acgtgtattg ctgtatctgc
     2521 gcggctgggg ctgccgcatt ggggtggaaa gaacctgtgc gctttcctgg tccacaggca
     2581 gaaaatgtcc gttataaaaa cgccggtaaa tcgtccccag agaaccgtta cgttgtttcg
     2641 tgatattaat ttctgctatg cctgtagcct gtgtatccgg gttgtacact tcatccctgt
     2701 aaagcatcag aatgatgtct gcatccgcct ctatttctcc ggaatttttc aggtctgagt
     2761 tcatgggacg tttattgggt ctggactcca caccgcggga gagctggctc agcgcaatca
     2821 acggaaaacc accggatttt gccaggcctt taagcccctt tgagatttca cccacggcaa
     2881 ggtcatgacg ccccgtggtt cgggttttta tcagcccgag ataatcaacc accaccagcg
     2941 ccgtttccgg atgtttaatc agatggtgtt tcgttgttgc gcatatctca tcaatggtca
     3001 ggttcgcctg gtccaccatc cagatattgc gcccggtcat ccgccccacc ccttgtgaga
     3061 aacgcgccca gtcttcatct tcaaagtgag ccacagattt caggcgtgat actggcatcc
     3121 ctccagccgc agacaccata cgttcaccaa tctggatgtt cgccatctcc atggtgaaca
     3181 gaagcacacc atgcccctgc tcagtcacct tgtcgatgat gtccagcgca agttcggttt
     3241 tccccatcga aggacgggcg gcaatgaata ccaggtctcc gggctccata ccgcctgttt
     3301 ttgcgtccag ttcatcaata ccggtcatca acgtcctgga tttctccagc ccctgattcc
     3361 ggcattcaac acgctcaacc acttccggaa gcacatcatc aatatgtacc ggctgaatga
     3421 cgccctttcc tgtcgacagt gtgaccatca tgttctgcgc atccttcagg gcatcttcag
     3481 ctgcttcaca ggtatgcgca tcacgtaatt tctgcagcgc ctcattcagt gttttttctg
     3541 catcgcgcag tgcggcattg cgccgcaacg ctgcaacata gtgctccagt gaagacttca
     3601 cccaggtttt acgcccggta tcagtaat
//
LOCUS       DAZOZD010000334         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOZD010000334 DAZOZD010000000
VERSION     DAZOZD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.338"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            68..799
                     /gene="traT"
                     /locus_tag="ACHUPB_004198"
     CDS             68..799
                     /gene="traT"
                     /locus_tag="ACHUPB_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR0760847.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1002..1739
                     /locus_tag="ACHUPB_004199"
     CDS             1002..1739
                     /locus_tag="ACHUPB_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760848.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            1790..>3627
                     /gene="traD"
                     /locus_tag="ACHUPB_004200"
     CDS             1790..>3627
                     /gene="traD"
                     /locus_tag="ACHUPB_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR0760849.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDV"
BASE COUNT          905 a          843 c          966 g          913 t
ORIGIN      
        1 ctctctgcaa tagtctcagt aataggtctg ttcaaaaaag agtctgttag ttaaaaggat
       61 attctggatg aaaacaaaaa aaatgatgat ggttgcactg gtcagttcca ctctggccct
      121 ttcagggtgt ggtgcgatga gcacagcaat caagaagcgt aaccttgagg tgaagactca
      181 gatgagtgag accatctggc ttaaacccgc cagcgaacgc acggtatttc tgcagatcaa
      241 aaacacgtct gataaagaca tgagtgggct gcagggcaaa attgctgatg ctgtgaaagc
      301 aaaaggatat caggtggtga cttctccgga tgcagcctac tactggattc aggcgaatgt
      361 gctgaaggcc gataagatgg atctgcggga gtctcaggga tggctgaacc gtggttatga
      421 aggcgcagct gttggtgcag cgttaggtgc cggtattacc ggctataact caaattctgc
      481 cggtgccaca ctcggtgtgg gccttgctgc tggtctggtg ggtatggctg cagatgcgat
      541 ggtggaagat gtgaactata ccatgatcac ggatgtgcag attgcagagc gtactaaggc
      601 aacggtgaca acggataatg ttgccgccct gcgtcagggc acatccggtg cgaaaattca
      661 gaccagtact gaaacaggta accagcataa ataccagacc cgtgtggtct caaatgcgaa
      721 caaggttaac ctgaaatttg aagaggcgaa gcctgttctc gaagaccagc tggccaaatc
      781 aatcgcaaat attctctgaa cctcgtcagg aggctgatct gaccggcctc cttgctgacg
      841 cctcgcgttg ctcgttgtcc aaccccggga tgtaacaaaa taaattttgt cacctcccgg
      901 cgtttccgaa ataaatatga aaacactgag cggttgtgca aattctttgt gcgaccgtga
      961 agtgttgccg gcgaagccgg gatcttccgg agaaagtcat tatgacatca gacaatatcg
     1021 ctgaatatat tggtgagaat aatcaattta ctgagaagcc tgcaactgaa taccccccgg
     1081 tgatacagga taatacagtg accactaaag actatctgta cagcaataat attgatactg
     1141 accttttagt ggttatcgga gggcttattg ttatattgtt tctcatgatg cttatcatgt
     1201 actttattaa ttctctcagg cataaggaat taactgaaat actgacaaaa atatcttttc
     1261 ctgaaaaatc cgggagcaat ttgcttaaac cggaggcggt cagctgtaat ttgttgtccc
     1321 tgtgtgtgct ggcaccggta ttctgtacac ccagaaagct ggctgaagtt tccagggaga
     1381 tcatcgtggc gagtgcgcag gaaattgcat ggacgccggg gagcagtcgt attgatggcc
     1441 accaggcgga gaatatcacc tgggcgattt catcgcagtc tcctgccgat aagtatgatc
     1501 tcatcaataa aacgtcctgt gtttctcctg acagtggccg tttttatgaa ccggacacag
     1561 ttctggatca ccgtgaacgg gtacgccttt ttattcagat ccagtttccg catgggagtg
     1621 actggtctga agtattgcgc ctgactgaca ttgttacaga gaaggtctct tccggcagga
     1681 ctgcaggcgg gcatcaccac cggcatacag ggtacacctg gtctgtttct gtgaattaac
     1741 cccacatcat ccttttctgc cctgttccag attttcccgg agtttcatca tgagttttaa
     1801 cgcaaaggat atgacccagg gcggtcagat tgcgtccatg cgtatccgca tgttcagcca
     1861 gatcgccaat atcatgcttt actgcctgtt tatttttttc tggatcctca ttggtctggt
     1921 cttatgggta aaaataagct ggcagacgtt tatcaacggc tgtatttact ggtggtgtac
     1981 gtcactggaa ggcatgcggg atttaataaa atcccagccg gtctatgaga tccagtatta
     2041 cggcaaaacc ttccggatga acgctgctca ggtactgcat gataaatata tgatctggtg
     2101 cggagagcag ctatggtccg cattcgttct ggctacagtt gtggcactgg ttatttgcct
     2161 gattaccttc tttgttgtct cctggattct ggggcgtcag ggtaaacaac agagcgaaaa
     2221 tgaagtcaca ggtggtcgtc agctgacaga caatccgaaa gacgttgccc ggatgctgaa
     2281 aaaagacggc aaggactccg atatccggat tggcgatctg ccgattatcc gggattccga
     2341 aatccagaac ttctgtctgc acggaacagt gtcgacgggt aagtcagagg ttattcgacg
     2401 tcttgccaac tatgcccgta aacgtggcga catggtggtg atttatgacc gttcgtgtga
     2461 gtttgttaaa agttactatg atccctccat cgataagatc ctgaatccgc tggatgcccg
     2521 ctgtgccgcc tgggatttgt ggaaggagtg cctgacacag ccggattttg ataatgtcgc
     2581 gaacacgctg atcccgatgg gaacgaaaga ggacccgttc tggcagggat caggacgtac
     2641 catttttgcg gaagcggcgt atctgatgcg taatgacccc aaccgcagct acagcaaact
     2701 ggtggacaca ctgctttcca tcaaaatcga aaaactgcgt accttcctgc gtaattcacc
     2761 ggcggccaac ctggtggaag agaaaattga gaaaacggcg atttccatcc gtgctgtgct
     2821 gaccaactac gtgaaagcca tccgttacct gcaggggatt gagcataacg gtgaaccctt
     2881 caccatacgt gactggatgc ggggtgtccg ggaagatcag aaaaacggct ggctgtttat
     2941 ctcgtcaaat gccgacactc atgcctccct gaagccggtg gtttccatgt ggctgtccat
     3001 tgccattcgt ggtctgctgg caatgggaga aaaccgtaac cgtcgtgtgt ggtttttctg
     3061 tgatgagtta cccacgttac acaaactgcc ggacctggtg gagatcctgc cggaagcccg
     3121 taagttcgga ggctgttatg tgtttggtat ccagtcctat gcccagctgg aagatatcta
     3181 cggtgagaaa gcggcggcca cgctgtttga tgtgctgaat acccgtgcct ttttccgctc
     3241 cccgagccat cagattgcag agtttgctgc cggtgaaatt ggtgagaaag agcacctgaa
     3301 agccagtctg cagtattcct acggtgctga ccccgtgcgt gacggggtat cgaccggtaa
     3361 ggagatggag cgccagacgc tggtcagtta ttccgacatt cagtctctgc cggatctgac
     3421 ctgttatgtc actctgcccg gaccgtatcc ggcagtaaaa ctctcactga aatatcagac
     3481 acgaccgaag gtcgctccgg agtttattcc gcgtgacatc aacccggaaa tggagaaccg
     3541 tctgagtgcc gtacttgctg caagggaagc agaaggtcgc cagatggcca gcctcttcga
     3601 accggaggtt gcctccggag aagacgt
//
LOCUS       DAZOZD010000335         3624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOZD010000335 DAZOZD010000000
VERSION     DAZOZD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.339"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..931
                     /locus_tag="ACHUPB_004201"
                     /pseudo
     CDS             1..931
                     /locus_tag="ACHUPB_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(1048..2391)
                     /gene="gdhA"
                     /locus_tag="ACHUPB_004202"
     CDS             complement(1048..2391)
                     /gene="gdhA"
                     /locus_tag="ACHUPB_004202"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0760850.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2627..2899
                     /locus_tag="ACHUPB_004203"
     CDS             2627..2899
                     /locus_tag="ACHUPB_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR0760851.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2865..3272)
                     /gene="nudG"
                     /locus_tag="ACHUPB_004204"
     CDS             complement(2865..3272)
                     /gene="nudG"
                     /locus_tag="ACHUPB_004204"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0760852.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            3359..>3624
                     /locus_tag="ACHUPB_004205"
     CDS             3359..>3624
                     /locus_tag="ACHUPB_004205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0760853.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          908 a          893 c          891 g          932 t
ORIGIN      
        1 tgctgctttg taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtgttcg
       61 cagcgataac atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc
      121 tttatctatt aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac
      181 aactgcatta ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc
      241 tacaattgat cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct
      301 ggccgcgaaa gcctgaacct ggtaaagata ccttccaata tcatgtgctt ccttttgact
      361 cgatcgatat catcagtaaa agacgcgagt ctttagagaa tcaatggggt atcgaagata
      421 gcgaaagtta ttgtgcctaa tggagcattt tctttctggc gaccatggag ccaatacctt
      481 taaagcaaac atggaggaag ccccagagca ggttatcgcc ttgttgaata aatttgctgt
      541 ttttccctca gactatatct ctgattgcgc taataatcgc tccggtaaat cctcggcgaa
      601 gctaatatgg gcggcggaat tatcatggat gacctcgata tcaagcacag cttttcaaaa
      661 cgggacaatt gaagaagaac tggcctggca ttatataatg cttgcttctc gaaaggcgca
      721 cgagttgttc gaaagcgaag aagattatca aaaaaatagt ctaatggggt ttctttactg
      781 gcatatctgc tgctatcgca gaaagttaac ggatgctgaa cttgaagcgt gttatcgtta
      841 cgacaagcaa ttttgggagc actacagtaa gaaatgccgt tggcccataa gaaatgttcc
      901 ctggggagca tcatccgtta aatactcata aacgcctgaa attttgccgg gggcgctgcg
      961 cttgcccggc ctacattaga gcgtaactgc aatgaattgt gcccatttgt aggcctgata
     1021 agcgtagcgc catcaggcat ttacaactta aatcacaccc tgcgccagca tcgcatcggc
     1081 aaccttcaca aaaccggcaa tgttcgcgcc ctgcacgtag ttggtttgct caccttcacc
     1141 accatgctca acacaggcat ggtggatatc cagcatgatg tgatgcaaac gtgcgtcaac
     1201 tttctcggct ttccagccca ggcgcgcagc gttttgtgcc atttccaggc ccgacgttgc
     1261 tacaccgcca gcattagccg ctttacccgg tgcaaacagt acgcctgcct gctggaacag
     1321 ttcagtcgct tcgatggtgg tcggcatatt tgccccttca gcgacggctt taacgccatt
     1381 ggcgataagc tgatgcgcgg cgtcaacatc cagttcgttc tgggtggcgc aaggcagggc
     1441 gatatcaacc ggtacagacc acggctgttg gccttcgaga tagaccagac caaattcttt
     1501 ggcgtaatct gccactcgac catcgcggct ggctttgatt tcgataagac gtgccagttt
     1561 ctctttcgtg aatccgcttt catcaactac agtgccgctg gagtctgacg cagtgatcac
     1621 acgagcacca aattccatcg ctttttcgat agcgtactgg gcgacgttgc cggagccaga
     1681 aacggaaacg cgcatccctt caaaacccat accgtggcgt tttaacattg cttctgtgaa
     1741 ataaaccaga ccgtagccgg tagcttccgg gcgaataaga ctaccgccaa atgaaaggcc
     1801 cttaccggtg aagacgcagg cggtattgtt ggagagcttt ttcatcatcc ccgccataaa
     1861 gccgacttca cgaccaccaa cgccgatatc acctgccgga acgtcggtat ccgcgcccag
     1921 atgacgataa agctcggtca tcagcgcctg gcaaaaacgc atcacttcac cttcgctttt
     1981 tcctttcgga tcgaaatcgc tgccgccttt accaccgccc atcggcaggg tagtcagggc
     2041 atttttgaag gtttgttcaa agccgaggaa tttgagaatg gaaaggttaa ctgacggatg
     2101 gaagcgcata ccgcctttgt acgggccgat ggcagagctg aactgcacac gccatgcacg
     2161 gttgacctgt atctggttgc gatcatcaac ccataccacg cgaaactgga tcacgcgctc
     2221 cggttcaacc agacgctcca gtaatgacat ctggcgatat tttggatttt gttcaagaaa
     2281 aggccagagt gtggtcatta cttcacgaac ggcttgcgcg aactcggttt gattcgggtc
     2341 gcgcttttgg acatggttga ggaatgactc cagagaatat gtctgatcca tagatataaa
     2401 acccttatat attaatacga ttgtgcttat gttgtttatg tcatgtgctt ttgcagtttt
     2461 cgactatacc atcaagaatg tgacggtaag caagaaaaaa atgacccggg aaagcaaatt
     2521 aataacgaga gtaatctcat aaccaaatgc gataactcag acgctaaagt tttgccaccc
     2581 ggctaccgtt atcagttgca gagacaataa cgttaaggag ttccctgtga gtcgagcatt
     2641 gttcgccgta gtattagcat ttccattaat tgcactggct aacccgcatt atcgtccgga
     2701 cgtggaggta aacgtaccgc cagaagtgtt cagttcaggc gggcagtcag cgcagccctg
     2761 tactcagtgc tgcgtttatc aggatcaaaa ttattctgaa ggggcggtaa ttaaagccga
     2821 aggaatactg ctgcaatgcc agcgggatga caaaaccctt agcactaatc cgctgctctg
     2881 gcggcgcgta aagccataaa cgcctctaat agtggaatgt cagcaggggc cagcggatat
     2941 tgcagcgcct cttcaggtga gcaccagacc agcgcctgat gttcatgtgc ctgtaacgtc
     3001 ccgtggaagt cgggtacgtg ccaggcatga agatggataa tccggcccga aacttctcgc
     3061 tgatggctgg caacatactc acccacagtt gcttcgatgc ccaattcttc gtttaactca
     3121 cgcaccagtg cctgctgctg gctttcatcc agctcgactt taccatcggc aaactcccat
     3181 aatcccgcct gatcgctctg ggcggggcgt tgcgcgagta aaattttgcc atcacgttca
     3241 atgatggcgg caacaacttc aatcattttc ataaattaca cacgggcaga taagcgaaaa
     3301 atgccataat atccgcactg ttgctcaatc agaaaggctt tatatttaag gagtgcctgt
     3361 gctagaccgc catcttcatc cccggattaa accgttgttg catcagtgcg tgcgggttct
     3421 tgataaaccg ggcattacgc cagatggttt aacattagtg ggatttgcca tcggcgtgct
     3481 ggctctgccg tttctggcgc tgggctggta tctggcggcg ctagtcgtca ttttgttgaa
     3541 caggctgctt gatggtctgg acggtgcgct ggcgcggcgc agagagctta ccgacgcggg
     3601 cggctttctt gatatttctc ttga
//
LOCUS       DAZOZD010000336         3597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOZD010000336 DAZOZD010000000
VERSION     DAZOZD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.340"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..70
                     /locus_tag="ACHUPB_004206"
     CDS             <1..70
                     /locus_tag="ACHUPB_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR0760854.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHUPB_004207"
     CDS             complement(71..175)
                     /gene="yoaI"
                     /locus_tag="ACHUPB_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0760855.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            345..791
                     /locus_tag="ACHUPB_004208"
     CDS             345..791
                     /locus_tag="ACHUPB_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0760856.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHUPB_004209"
     CDS             complement(748..1569)
                     /gene="nimR"
                     /locus_tag="ACHUPB_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR0760857.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1666..2847
                     /gene="nimT"
                     /locus_tag="ACHUPB_004210"
     CDS             1666..2847
                     /gene="nimT"
                     /locus_tag="ACHUPB_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR0760858.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2902..3249
                     /locus_tag="ACHUPB_004211"
     CDS             2902..3249
                     /locus_tag="ACHUPB_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0760859.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHUPB_004212"
     CDS             complement(3271..3525)
                     /gene="yoaF"
                     /locus_tag="ACHUPB_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0760860.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          815 a          919 c          893 g          970 t
ORIGIN      
        1 tattttgaaa accacggatt atttgcgcat tgcgcaacca gaactgctca atttccgccg
       61 cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga caaaaccagc acaacagcaa
      121 tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa catttgatcg ttcatagcat
      181 gcccccggga gatagctgtt ttcacgttat tgttagcgtg cacaaatggc agtttgatga
      241 caattcgcct gattttttat gcaaaaaacg taaagatttt ctacttcctt cctgcagcaa
      301 acgtaaagta agcaggctta ttattttttg gcaaggaaac cacgatgttt gatgtcactt
      361 tgctgatcct gctcgggtta gctgcgctgg gctttatcag tcataacacc actgtcgccg
      421 tttcaattct ggtgttgatc attgtccgcg tcacaccgtt aagcaccttt ttcccgtgga
      481 ttgaaaaaca ggggctaagt atcgggataa tcattctgac gattggcgtt atggccccca
      541 tcgccagcgg gacgctaccg ccctcaacct tgattcactc gtttctgaac tggaaatcgc
      601 tggtggcaat tgcagtaggc gtgattgtct cctggctggg tgggcgcggc gtgaccttaa
      661 tgggcagcca gccgcaactg gtcgccgggc tgctggtagg cactgtttta ggcgtggcgc
      721 tgtttcgcgg cgtaccggtc ggcccgctta ttgccgcggg tctggtttcg ctgattgtgg
      781 ggaaacagta gttaatcccg caatatatcg gcctggcgtt tgccccaggc ctttcttaaa
      841 catagtaata aaggccgttg ttgaatcata ccccagcgta tgagcaactt tctgtacagt
      901 atcgcctttc actaatccct gaagtgccat aatcagctgt aactgctgac gccactggcg
      961 aaaactcaac cccgtctctt ttactatcag tcgcgcaaga ttgcgttcac tcatcgcaaa
     1021 aaagccagcc cattgcccta atgaccccca ctcgacgggc cctttcgcca tcatctccac
     1081 catcgtgcgg attttaggat gagaagaaac gggcaggtgc agctgttgtt gtggctgctg
     1141 agggagttca tcaaacagga cctgaattaa tctgcgggtc atcggctcag cacgctgagt
     1201 cgtcgtcctg tttgccaggg ttaaaatcaa ttcccggcat aagggcgata ttttcaatgt
     1261 gcaacatgtt gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc
     1321 gttagctgtc acttgattgc tatgttccac cccgcccggt atccaaaccg catattgcgg
     1381 cggcaccatc cataaagcat tttccaccgt acaggtaatt gcaccatgta atgctagtat
     1441 cagctgtcct ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat
     1501 acaaaatgcg accgcggctt catggtgtcg gtcgggttca tagccattga gattcaacct
     1561 gtgcatcatt ttgtccgaat ttagcgataa tttgtcattt tagcttgatt caacataaca
     1621 ataaaaacgg taaggtacag cctcgtttgt aacaatgaga agcatatgac ctgttcaact
     1681 tcattaagcg gcaaaaacag gattgtcctt atcgctggca ttctgatgat tgccacaaca
     1741 ttacgcgtca cctttatcgg cgcagcaccg ttactggata cgattcgttc cgcttactcg
     1801 ctgacgacag cgcaaaccgg cttattgacc accctgccat tattggcctt tgcgctaatc
     1861 tcacctttgg ccaccccggt agcgcgacgt tttggtatgg aacgtagcct gtttgccgcg
     1921 ttacttttga tctgtgctgg tatcgcaatt cgctctctcc cttcgcctta cttattattt
     1981 ggcggtacag cggtcattgg cggtgggatt gcattaggca atgtcttact gccaggatta
     2041 attaaacgcg atttccctca ttccgtcgcc agacttaccg gcgcatattc cctgacaatg
     2101 ggagctgcag cggcactggg atcggctatg atagtgccgc tggctttgaa cggttttggc
     2161 tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc tggctctttt tttatggctg
     2221 ccacagtggc gaagccaaca acatgcaaat ttgagtacct cgcgcgcctt acatactcgg
     2281 ggtatctggc gttcgccgct tgcctggcag gtaacattgt ttcttgggat caactcactg
     2341 atctattacg tgattattgg ctggcttccg gcgatcctca tcagtcacgg ctatagcgaa
     2401 gcacaggcgg gttcactgca tggtttgctg caactagcca cagcagctcc cggtttgctg
     2461 atccctcttt tcttacatca tgtgaaagat cagcgtggta ttgcagcgtt cgttgccttg
     2521 atgtgcgcag tgggcgctgt tgggctctgc tttatgccag cgcacgcgat cacctggact
     2581 ctgcttttcg gttttggttc cggcgcaaca atgatactgg ggttgacgtt cattggtctt
     2641 cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga tggcacaatc cgtcgggtat
     2701 ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac acgatgctaa cggtgactgg
     2761 tccgtaccac tgatgggtgt tgcaatactt tcactactga tggcgatttt cggactttgc
     2821 gccgggagag acaaagaaat tcgctaatat ccggtgctat agtgacgtga caaatcatgc
     2881 gtgaaaggga gaacaaacac gatgaatatt cagtgcaaac gcgtttatga tccggctgaa
     2941 cagagcgatg gttatcgcgt actggtcgac cgcctctggc cgcgcggtat caaaaaaacc
     3001 gatttagccc ttgatgagtg ggataaagaa atcacgccgt caacggaact gcgcaaagcc
     3061 tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc aatatcttgc agaactggcg
     3121 caacacgagc aagaaggaaa gcggctggcg gacatcgcca aaaaacagcc gctgaccctg
     3181 ctctacgcag caaaaaacac cacgcagaac catgcgctgg tgctggccga ctggctacgt
     3241 agcttgtgat tttagtacag catccggcgg ttatttttca ccagccggat ggtcccgtcg
     3301 ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat ttgcaattgt tgctgacccc
     3361 ttgcgctgtc gacactggaa tgagcgtccc gcccttctgc tggcaataga ccgacgccgg
     3421 atttgccata ccaatctgcg gcggtttagg tacttctggc tgagaagagg ttgaacaacc
     3481 agccaggacc agcaagcaag gcaaagcaaa actgataatt ttcatttatt gatctcactc
     3541 atttatctac gcttagagta tcgcggtatc gttttgtttt gcagcactat ttttatt
//
LOCUS       DAZOZD010000337         3578 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOZD010000337 DAZOZD010000000
VERSION     DAZOZD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.341"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUPB_004213"
     CDS             <1..197
                     /locus_tag="ACHUPB_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760861.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            208..375
                     /locus_tag="ACHUPB_004214"
     CDS             208..375
                     /locus_tag="ACHUPB_004214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760862.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            372..716
                     /locus_tag="ACHUPB_004215"
     CDS             372..716
                     /locus_tag="ACHUPB_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760863.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1008..1163
                     /gene="hokD"
                     /locus_tag="ACHUPB_004216"
     CDS             1008..1163
                     /gene="hokD"
                     /locus_tag="ACHUPB_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0760864.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1384..1470
                     /locus_tag="ACHUPB_004217"
                     /pseudo
     CDS             1384..1470
                     /locus_tag="ACHUPB_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1467..1739
                     /locus_tag="ACHUPB_004218"
     CDS             1467..1739
                     /locus_tag="ACHUPB_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760865.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1741..2799
                     /locus_tag="ACHUPB_004219"
     CDS             1741..2799
                     /locus_tag="ACHUPB_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0760866.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2800..3180
                     /locus_tag="ACHUPB_004220"
     CDS             2800..3180
                     /locus_tag="ACHUPB_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0760867.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3177..>3578
                     /locus_tag="ACHUPB_004221"
     CDS             3177..>3578
                     /locus_tag="ACHUPB_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0760868.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          809 c          980 g          821 t
ORIGIN      
        1 catcaccgaa aaaaattggc ttttacacca ttggcgataa aagcatttac ctgtatgacc
       61 tacgccgcat ggatgaaatc atggaggctc ttgataatcg ttcgtcgatg gattggtgtg
      121 ttgctgttca tgatatgaat gcagggtttg atgaaaagat tttgttcccc tcatcagttg
      181 aaagcactgc gggttaagga gtaacacatg accactatta ccaaagaacg tattgaattg
      241 ttcattaaaa acccgcttga aaacgggctt acccgtggtg aacaaatgga actggcacgg
      301 attgcgctgg catcgctgga agcagagccg gttgtgttct ggtttgaaaa atatcaagaa
      361 ggggctacgg catgacgact tttaccagag agcagttaat agctcacgca gaggagacta
      421 ttgaagcaca gagactgtgc ataccgggca caatcgacca tgacatcatc cgcacatata
      481 agatggatat tgctgttctg gaaatcgcac tggtatcgct ggcagcagag ccagccggta
      541 aattgcatga atacaaacca gtgggatatc agcgtctggt cgatgagtta accatgctgg
      601 taaagcagtt aacctggcaa ctgaggaaag cgaagccaga ctgcaaatta ccggataagg
      661 cgatgagtta tctggagcgg aacggactga taagcgtgga ggatatttta cgatgacctg
      721 gcctgaagca ttaacaacgg taggaattgc gatggcggtg gcgctggtgg tgtattcgat
      781 ttgccgctgg ggataaaaac ggtttgcggg aaaaggagag ttaagtagaa ttgcagcggg
      841 tgcttgaggc tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt
      901 taacattacg cgtccggcaa gacgcttaac attaatctga ggctcaatct atgaacggca
      961 aatctaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     1021 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     1081 cgaggaaaga cctctgcgag gtacgaattc gaaccggcca gacggaggtc gctgtcttca
     1141 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
     1201 tgggtcaggc atcctcaatg cacccacact taacccgctt cggcgggttt ttgtttttat
     1261 tttcaacgcg tttgaagttt tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg
     1321 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1381 ttgatgaaaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1441 gttgagaatg gcgctattgt cagccactgc cttccagact acacaattct gataattcag
     1501 ccgtctttgc cagcaggcac gggcggcgct ctcacgcatt taaatctgat tggtaccagc
     1561 atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc aggcgcggat
     1621 gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc gtcaggctcc
     1681 cttactccga acggccaacg cgtccgtccc gcacattcca gcaacggatc tggaggtaat
     1741 gtgcgggtat tacttcgacc tgttctggta ccggaactcg gtctggttat cgttaagcca
     1801 ggccgtgaat caatgtcagc attccataac ggcagaatac tggtggagcc ggaaccaaaa
     1861 agcatgcgta atctgccgtc cggggtcgtt cctgccgctc gccagccgct ggtggaagac
     1921 aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg tggtgctggt
     1981 gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca cggcgattat
     2041 catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt gtgctggcac
     2101 tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc tcatcaaaac
     2161 ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca ggagcgtgaa
     2221 ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga cgcgctaccg
     2281 gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaattcgctc tgtgtacagt
     2341 gaaagcgaca tcataccggg agagcagaca gccaccagca tactgaagca gcgcacaaaa
     2401 aatattgcgt tactgcctca cgtccaccag caacagaacc caccacagga aaagacggtg
     2461 gtcagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca gaaatcacaa
     2521 cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat gacgtgtggc
     2581 aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat gggaacaaag
     2641 gctgatgatt tgtttgttat tccgttatgc cgtaaatgcc gtaacgaact acatgccgga
     2701 gtaaaagatt ttgaagagaa acacggcagt cagctgttgt tgctgattcg ttttttaatg
     2761 cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa tgaccgagcg catagaattt
     2821 gttttacctt acccgccgac ggtgaacact tactggcgtc gtcgtggcag cacatatttt
     2881 gtatcaaaag ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg ccagcagcgg
     2941 ctgaaattaa gcctgtccgg aaggttggca ataaaaatta ttgcagaacc accggataag
     3001 cgccgccgtg acctggacaa tattctgaaa gcgccgctgg atgcgctgac gcatgcgggg
     3061 ttgctaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtgctca gccagtatct
     3121 ggtggacgtc tgggggtgaa gatttatccc ataatgcttg aagggcaggt caaaaaatga
     3181 aactggaaga tttaccgaaa tactactccc caaaatcccc cggcctgact gatgcatcgg
     3241 cctcaacgtc gaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac
     3301 agaatcgggc tgagatgggg gtttctgcgt tccttgggaa aatgggcatt agtatgaatg
     3361 acagagagcg ggcaacagaa ttgctgacag aatatgcact cagtcggtgt gatcgcgtgg
     3421 cggcgttaag aaaactcccg gcagaaataa aaccggcagt gatgcgtatt atggcttcgt
     3481 atgcgtttga agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc tgctgtcacg
     3541 gaaaaaaatt tattgaaagc gaggttttta caaacaag
//
LOCUS       DAZOZD010000338         3559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZOZD010000338 DAZOZD010000000
VERSION     DAZOZD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.342"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHUPB_004222"
     CDS             129..1376
                     /locus_tag="ACHUPB_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR0760869.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUPB_004223"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUPB_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR0760870.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHUPB_004224"
     CDS             complement(2228..2572)
                     /locus_tag="ACHUPB_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760871.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHUPB_004225"
     CDS             2814..3416
                     /locus_tag="ACHUPB_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760872.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAZOZD010000339         3558 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOZD010000339 DAZOZD010000000
VERSION     DAZOZD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.343"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..353
                     /locus_tag="ACHUPB_004226"
     CDS             <1..353
                     /locus_tag="ACHUPB_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0760873.1"
                     /translation="RWYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVVIV
                     ELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWL
                     NTTGADQQSPEHRS"
     gene            593..853
                     /locus_tag="ACHUPB_004227"
     CDS             593..853
                     /locus_tag="ACHUPB_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR0760874.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1077..1151
                     /gene="tap"
                     /locus_tag="ACHUPB_004228"
     CDS             1077..1151
                     /gene="tap"
                     /locus_tag="ACHUPB_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR0760875.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1144..2001
                     /gene="repA"
                     /locus_tag="ACHUPB_004229"
     CDS             1144..2001
                     /gene="repA"
                     /locus_tag="ACHUPB_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0760876.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2701..2892
                     /locus_tag="ACHUPB_004230"
     CDS             2701..2892
                     /locus_tag="ACHUPB_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760877.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            2965..3216
                     /locus_tag="ACHUPB_004231"
     CDS             2965..3216
                     /locus_tag="ACHUPB_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFR0760878.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            3213..3500
                     /locus_tag="ACHUPB_004232"
     CDS             3213..3500
                     /locus_tag="ACHUPB_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0760879.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          942 a          872 c          908 g          836 t
ORIGIN      
        1 agcggtggta tctgtgtccg caggtacggg tcgcggatat cgtccagctg aacgggaata
       61 tccggccacg atcacgccag tggtggcagt tattcaggat ggtgtctcag tggcatgttg
      121 atgtggttat cgttgagctg cgttcgttca gtattgttgc agcagtagag ctggatgatg
      181 ccagccattt acgaccggaa cgcagacgcc gggatattct tctggaagag gttctgaggc
      241 aggctggtat tccgttgctc agaagccacg atgccagaaa actgctgcag atgaccggag
      301 aatggctgaa tacaacaggg gctgatcagc agtcaccgga acatcgtagc tgacgccttc
      361 gcgttgctta gttgtccaac cccggaaacg ggaaaaagca agttttcctc gctcccggcg
      421 tttccataac tgaaaaccat actatttcac agtttaaatc acattaaacg acagtaatcc
      481 ccgttgattt gtgcgccaac acagatcttc gccacaattc tcaagtcgct gatttcaaaa
      541 aactgtagta tcctctgcga aacgatccct gtttgagtat tgaggaggcg agatgtcgca
      601 gacagaaaat gcagtgactt cctcattgag tcaaaagcgg tttgtgcgca gaggtaagcc
      661 tatgactgac tctgagaaac aaatggccgc agttgcaaga aaacgtctta cacacaaaga
      721 gataaaagtt tttgtcaaaa atcctctgaa agatctcatg gttgagtact gcgagagaga
      781 ggggataaca caggctcagt tcgttgagaa aatcatcaaa gatgaactgc agagactgga
      841 tatactaaag taaagacttt actttgtggc gtagcatgct agattactga tcgtttaagg
      901 aattttatgg ctggccacgc cataaggtgg cagggaactg gttctgatgt ggatgtacag
      961 gagccagaaa agcaaaaacc ccgataatct tctttaactt tggcgagtca gaaagattac
     1021 cggggcccac ttaaaccgta tagccaacaa tcaagctatg cggggagtat agttatatgc
     1081 ccggaaaagt tcaagacttc tttctgtgct cgctccttct gcgcattgta agtgcaggat
     1141 ggtgtgactg atcttcaaca aacttattac cgccaggtaa agaacccgaa tccggtgttc
     1201 acaccccgtg aaggtgcccg gacgcttccg ttctgcggga aactgatgga aaaggcggtg
     1261 ggcttcacct cccgttttga tttcgccatt catgtggcac atgcccgttc gcttggtctg
     1321 cgtcggcgca tgccaccggt actacgtcgc cgggccattg atgcgctgct gcaggggctg
     1381 tgtttccact atgacccgct ggccaaccgc gttcagtgct ccatcaccac gctggccatt
     1441 gagtgcggac tggcgacgga gtctgatgcc ggaacacttt ccatcacccg tgccacgcgt
     1501 gccctgacgt tcctggcaga gctggggctg attacctacc agacggaata tgacccgctt
     1561 atcggctgca acattccgac cgacatcacg ttcacaccgg cgctgtttgc cgccctcgat
     1621 gtatcagagg aggcggtggc gtccgcgcgc cgcagtcgtg tggaatggga aaacagacag
     1681 cgcaagaagc agggactgga taccctgggt atggatgaac tgatggcgaa agcctggcgt
     1741 tttgtgcgtg agcgtttccg cagttaccag acagagctta agtcccgtgg aatgaagcgt
     1801 gcccgtgcgc gtcgtgatgc ggacaggcag cgtcaggata ttgtcaccct ggtgaaacgg
     1861 caactgacgc gtgaaatctc ggaagggcgc ttcactgcca gtcgtgaggc ggtaaaacgt
     1921 gaagtggagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
     1981 ctggccacag cttccccctg aaagtgacct cctcagaata atccggcacg cgccggaggc
     2041 attcgcacgc ctgaagcccg ccagtgcaca aaaaatcagc gctacataca aaaaacaacc
     2101 tcatcatcca ccttctggtg catccggtcc cccctgtttt tgatacaaaa cacgcctcac
     2161 agacggggaa ttttgcttat ccacattaaa ctgcaaggga cttccccata aggttacaac
     2221 cgttcatgtc ataaagcgcc agccgttagc tttacagggt gcaatgtatc ttttaaacag
     2281 ctgtttatat ctcctttaaa ctacttaatt acatccattt aaaaagaaaa cctgttcact
     2341 gcctgtcctg tggacagaca gatatgcacc tcccaccgca agcggcgggc cccgaccgaa
     2401 gccgatttag ttacaacatg caaacataaa cccctaaaaa acccaagtcc gacgcagaac
     2461 tgaaaccaca acgcccctcc tcataactga aaagcgccgc tgcccccgcc cgaagggcgg
     2521 gaacagagtc gcttttaatt atgaatgttg taactacatc gtcatcgctg tcagtcttct
     2581 ggctggacgt atcgaataca cgctcgtaag cggccctgac ggcccgctaa cgcggagata
     2641 cgccccgact tcgggtaaac cctcgtcggg accactccga ccgcgcacag aagctctgtc
     2701 atggctgaaa acgggtatgg cttagcaggg ctggggatgg gtaaggtgaa atctgtcaat
     2761 cagtaccggc taacgccggg cttcggcggt tttactccgg tatcacatgt aaccgcagcg
     2821 tgccgcctta catgccgctg gcgcggcatc agaataatac aagcagcatt taatgcattt
     2881 gcaaaagtgt gagtaataaa gacttgttaa gtggtcgttt cagggatatc gttaagactt
     2941 gttaaacagt ctcaaaggtg cgttatgcct aacatcattc tgtctgagac cagtgcaagt
     3001 gtcagtgaac tgaaaaaaaa tcctatggct acagtcagtg ccggagatgg ttaccctgtc
     3061 gccattctta accggaatca gccggcattc tactgtgtgc ctgctgaact gtatgaaagg
     3121 atgcttgatg ctctggatga ccaggaactg gtgaaactgg ttactgaacg cagcaatcag
     3181 ccactgcatg atgtggacct ggacagttat ttatgactta tacggtaaaa ttcagggatg
     3241 atgcgctcaa ggaatggctg aaactggata agaccattca acaacagttt gtgaaaaaac
     3301 taaaaaaatg cagcgaaaat cctcatatac cgtcggcaaa actaagaggg ctaaaggact
     3361 gctacaaaat taaactacgt gcatcgggat ttcgtctggt ttatcaggtg attgacgata
     3421 tgttaattat tgcggttgtt gcggtaggaa aacgagaacg tagtaacgtt tataatctcg
     3481 ccagtgagag aatgagatag taacatataa ataacctttc ccgcagataa ctcagaattg
     3541 ttattttctc tgtggact
//
LOCUS       DAZOZD010000340         3544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOZD010000340 DAZOZD010000000
VERSION     DAZOZD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.344"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            342..800
                     /gene="traH"
                     /locus_tag="ACHUPB_004233"
     CDS             342..800
                     /gene="traH"
                     /locus_tag="ACHUPB_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HFR0760880.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            797..1615
                     /gene="traI"
                     /locus_tag="ACHUPB_004234"
     CDS             797..1615
                     /gene="traI"
                     /locus_tag="ACHUPB_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HFR0760881.1"
                     /translation="MKALTSLILPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLISTEGGSR"
     gene            1612..2760
                     /gene="traJ"
                     /locus_tag="ACHUPB_004235"
     CDS             1612..2760
                     /gene="traJ"
                     /locus_tag="ACHUPB_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFR0760882.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPNLLEAMVCKSGLG
                     FVCGETGSGKSTLCAALYCYIKKNYPNAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VASFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            2757..3047
                     /locus_tag="ACHUPB_004236"
     CDS             2757..3047
                     /locus_tag="ACHUPB_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR0760883.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTICTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            3062..>3544
                     /locus_tag="ACHUPB_004237"
     CDS             3062..>3544
                     /locus_tag="ACHUPB_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFR0760884.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     P"
BASE COUNT          880 a          915 c          882 g          867 t
ORIGIN      
        1 acgggcacat gtgcctgtct gaattgcagg ttatccggac gagggccggg aactgtttca
       61 ggtacaagcg ccggtacgtt cgattcataa atcgtcgagt catccatcat ggatcgattc
      121 ggcgcggagc gttaaagatt acgctcacat tcatattatg tctgacgacc aatcaaccag
      181 ccagtatcag gaataatcac cttcagtaaa ctatgaggct atttacctgg ccgcactggc
      241 atttatttcc ggtaacgcaa gtatagccgg aaacaaacat catcctgtct gtaccggagc
      301 accggtatat cccattctga tttaactgct caggaattat tatgaaccgt attatacctg
      361 cggggtgtct tgccgccctt tttctggctg gctgcaactc cccttctcag catactcctg
      421 ttccgcgtaa ctacggtgct gcctctgatg tggcggttac ccggcacact cagtacacat
      481 tatgggacaa aggtgtgttt tccggccaac ttccggtttc aacggggtct catcttctga
      541 cagctaacag cgatcgcctt tcctttgact gggaaggtga tgcgattgaa ttactgaacg
      601 aactggccag agtcaggggg atgcagttta actacaatgg tgtccgcctg ccgttaccag
      661 tcaaccttca cgtccgggat atgacgtttt ccaatacgtt gagacttatt gaagcccaga
      721 cagcctggag agcaacaatc catcaatatc cgggcttgtt acaggtcagc ttcatgcagc
      781 cggagaaccg gaaaaaatga aagcactcac ctctctcatt ttgccagtaa tactgcttac
      841 tggttgttct tcttccccta ccatctcgcc aggtggtgtc aatacgccac cacctgacat
      901 taatgcatgg cttaaaccac gaagtgaaaa gccggatagt ctgtcagaaa cacgctggaa
      961 aatgctgacc gacgcaggta aaacgcttgg atttcgtggt ggcaaggctc agcgctcatg
     1021 ggagctgatt caggcactca acgcccggga aagcacactt aatgccttat acgatttccg
     1081 gccactgatt agcccggaag gctggctacc accggttatt gatgaagcac aggacgtagc
     1141 tcatatcacg ccagatcaaa tccggacctc ttcccgggtc tggaccatca ttcgccctga
     1201 acgctttgtc agcaatccac cgggctggcg tgactggctt ctccgggggc tttcaacaac
     1261 agccactccg ggtacagaag gcagcgtcgt tccagaagac agcgtgcaac gaaaagtgtg
     1321 ggaaaccgca ttacggcagg gatggcagga aggaagacaa aacgccgacc tgacgcttga
     1381 agccaaccag aaaaccctca cccgtgatta ccggggcatg atgctgtact cacttctctg
     1441 gcgtcagggc atgatcaccc gtccggatgt ttctgatcaa atgcaaaccg ttacaggtga
     1501 cgggaaaaaa ctggtcactg gagatcgtgt tcgtcgcctt aaaaatcatg cggaattcaa
     1561 tcttcagaaa tctcactggc gtccgcttat aagcactgaa ggaggctccc gatgaatatt
     1621 cctgatgagt ttggtctttt tccgtttcag cgtttcacag cagatgaact acggcatttc
     1681 tttgtctggt gtgccgcaca taaagtcagt gatgtggatc tgaccggtgg gtcaccagtt
     1741 tctgtcagtc gctttggccg acgggtgcgt tgttccagtg caacgttacc aaccacactc
     1801 atgtcctccc tgattgatga actgttcggt cgggaagtca ttccccgtgt tctggcaggc
     1861 aatccggttg acaggacgat acagattaac ggggatgcat ccggtcgcta tggtcttaaa
     1921 cgcggagaac gcatccggtt acgctctcac ctcattcagg gcacatctgg tgcggaggag
     1981 aaagcaatat caataaccat gcgtgtcatc ccgacagaaa taccggacat cctctccatg
     2041 aacattgaac caaacctgct tgaagcaatg gtctgcaaaa gcggtctggg atttgtatgt
     2101 ggtgaaacag ggtcaggaaa atcaacgctg tgtgcagcac tgtactgtta cattaagaag
     2161 aattatccca acgccaaaat cgtgacatat gaagacccgg tggaatatat cctgggaaat
     2221 gaaaacgatc tgctcccacc gcatcaggca gaaatcgggc gtgatgtggc cagttttgct
     2281 gccggactgc gttctgctgt acgacgtaat cccgaaatta tcggtgtcgg tgaaatcagg
     2341 gataacgaaa ccgccgacgc agcggtacag gcaggcaaca ccgggcatta ctgcctgagc
     2401 accatgcata cgaaatctcc tggtgaaacg ctggcacgtc ttcttgggct gtttccccct
     2461 gtcatcaggg attcgatggc ctgggctgtc ctgtcactgt tgcagttcat tctggttcag
     2521 gttctggtca gaacaaatga cggtggtcgt aaagccgtcc gggaatatat cgtcattaac
     2581 gatgagcttc gcgacaatct ttccggaatg ccacatgcag agtgggggca tcacattgat
     2641 gccataatcc ggcaggagaa gcgccgtatc cgggaccaga ttctggaaat gtatatccgg
     2701 aatgaagttg accgaagaga agcgattctt tttatcccgc caggggagtt acggtcatga
     2761 aaagagaagg tttctggcga tatgcaacac cgccagtcaa catccagggc gtacctctgc
     2821 cgttcaccat gatttatctg tttatatgcc cctttccctc caaaacaacc ttctggattt
     2881 gcaccggcat cattttattt ttcgttattc tcgaccgttc aggctggact gtcaggacga
     2941 tctgtactcg cattttcagc attctgagag gggccatcgc ctccggtcgt ccatggtggt
     3001 atcgccacca tacggaatca ccacgagact ggactggcct cgattaaatt ctggagtctt
     3061 tatgtcaggt aaaaaaacta tcgttactct gctccgggta tcgctactgg catgcccgtt
     3121 actgttcacg acaccatcgt ttgcaatgat cgacacgcca tccgtgaagg tcggcttttc
     3181 tcctgaagga agcgcatccg ctctggtgct tgacacaatc aacagtgcgg aaagctccat
     3241 caggatgatg gcatattcct tcaccgaccc ggatgtaatg catgcgctgg caaaagcgaa
     3301 aaaacgcgga gtggacgtcc gtattgttgt tgatgacaag gggaatacga accgggcaag
     3361 tcaggaagcg atgaaatata tcaacctgct ggacattcct ctccggactg tagatgcctt
     3421 ccccatccat cacgacaaag tcatcattgt tgacggaaac acggttgaaa ctggctccta
     3481 taacttctct cgtgctgctg cacgcaagaa ttcagagaat gtcgttgtgc tcaagaatat
     3541 gccg
//
LOCUS       DAZOZD010000341         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOZD010000341 DAZOZD010000000
VERSION     DAZOZD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.345"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUPB_004238"
     CDS             complement(<1..140)
                     /locus_tag="ACHUPB_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0760885.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUPB_004239"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUPB_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0760886.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUPB_004240"
                     /pseudo
     CDS             complement(696..2151)
                     /gene="xylB"
                     /locus_tag="ACHUPB_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(2223..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUPB_004241"
     CDS             complement(2223..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUPB_004241"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR0760887.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          923 a          983 c          783 g          854 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaagaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc ccccccccgt
      901 acggtaatcg agcttctgac cgctgatatc cgccagcatc tgacgccagt actcactacg
      961 cgccccgcca ccaatcaacg taacactttg cggtttaatg ccgcaggcat gcacgacatc
     1021 catgccatct gccagcgcat aacccacgcc ttccagcact gctcgcgcca gttcattggg
     1081 gccatgttga tgagtcaaac caaagaaaac ccccttcgcc tggggattat tgtgtggtgt
     1141 acgctcgccg gaaagataag gcagaaacca aactggctcg acactttcat cagcctgttg
     1201 agctgcagcg attaaagctg ggacattgca caggccggtt aatttcgcgg cccaatccag
     1261 acacgatgct gcactcagca ttacagacat taaatgccaa cgctgcggta gcgcatggca
     1321 aaagctgtgt acagcgcttt ctggcttgct taagaaccct tcgctgacag caaaatagac
     1381 ccccgacgtc cccagcgata acattgcctg attagcatca accattccca caccaactgc
     1441 accagctgca ttgtcgccac cgcctgcgac aactggcacc gtcgccatac cccacgcttt
     1501 cgcaacttca ggtaacaaag caccagtaat ttcgctgcct tcgtataatg cgggcatctg
     1561 gtcacgagat aagtcgcaag cctgcagcat gacgtcactc cagtcacgct ttgcgacatc
     1621 cagccacatg gtgccagctg cgtcagacat atcgctggca aactcccccg tcatacgcag
     1681 acgcaagtaa tctttcggta ataatacttt gtcgatttga cggaatatct ccggctcatg
     1741 ccgctgaacc catagcaatt taggcgcagt aaatccgggc atcatcaggt tgccggtaat
     1801 cactcgtgat tgcggaactc tcgcttccag caaagtgcac tcttgcgcac agcgcccgtc
     1861 gttccacaaa atggcagggc gcaatacccg ttgttgagca tccagtaagg ttgctccatg
     1921 catctggccg gcaataccca atgctttaac gtcctgcaga gaatgctgat cgcccagagc
     1981 tttcattgcg cgatcagttg cctgccacca ctgttccggg tcttgttccg accagagtgg
     2041 atgcgggcgc gaaacggtca gcttttccgt ttgcgaatca accacctcac cctgctcgtt
     2101 gagcaaaata acttttacgc ccgaggtgcc aagatctatc ccgatataca tatcgatcgt
     2161 tccttaaaaa aatgcccggt atcgctaccg ataaccgggc caacggactg cacagttagc
     2221 cgttatttgt cgaacagata atgatttacc agattttcca gttgctcctg gcgaccactc
     2281 tgatgcaccg gagacaaatt atgttcctga gcatatttgg ctaaatctgc cagtgacatt
     2341 tggcctttca ggatttgctg gcccaattcg ctattccagc cggaataacg ctgcgcgatg
     2401 cgtttatcca gctcgccatc ttcaatcatg cgcgctgcaa ttttcagcgc cagtgccatc
     2461 gtatccatcg cgccgatatg accgtaaaac agatcatatt tatcagtact ttgacgacgt
     2521 actttggcat cgaagttcag accaccggtg gtgaaaccgc ctgctttgag aatttcatac
     2581 atcaccagcg cattctcttc cacactgttc gggaactggt cggtgtccca gcccagttgc
     2641 gcatcgccac ggttggcgtc gacagaaccg aacaggccaa gcgcaatggc ggtggctatt
     2701 tcatgatgga aagagtgacc tgccagcgtc gcgtggttag cttcaatgtt cagtttaatc
     2761 tctttttcca gaccaaactg tttcaggaag ccatagaccg tcgcggcatc gtaatcatat
     2821 tgatgtttag tcggttcttg cggtttcggt tcgataagca acgtgccctg gaagccgatt
     2881 ttatgtttat gctcaaccac catctgcata aagcggccca gttgttcacg ctcctgacgc
     2941 aagtcggtat ttaacagcgt ttcgtaacct tcacgaccac cccacaggac atagttttca
     3001 ccgcccaatt tatgggttgc ttccatcgct gtaacaactt gcgttgccgc ccagctgaag
     3061 acttcaggat ctgggttcgt cgccgcaccc gcgccatagc gagggtttgt aaagcagtta
     3121 gcggttcccc acagcagctt cacgccgctc tcttcttgct tggctgccag gacatcaacc
     3181 atttgcgcaa aattattgat gtactctttt aacgacgcgc cctcagggga aacatccaca
     3241 tcgtggaagc aataaaatgg cacatgtaac ttgtggaaaa actcaaatgc gacatctgct
     3301 ttacgcttcg ccaacgccag tgcctcacca ggctgctgcc acggacgatt aaacgccccc
     3361 acaccaaaca tgtccgcccc gttccagcag aaggtgtgcc agtagcaggc ggcaaaacgc
     3421 aagtgctctt ccatacgctt acccaacacc agttcgtcgg gattgtagtg acggaatgct
     3481 aacgggtttg aggattttga gccttcataa cgaacgcgat cgagctggtc aaaataggct
     3541 tgc
//
LOCUS       DAZOZD010000342         3520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOZD010000342 DAZOZD010000000
VERSION     DAZOZD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.346"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..168
                     /locus_tag="ACHUPB_004242"
     CDS             <1..168
                     /locus_tag="ACHUPB_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0760888.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="ACHUPB_004243"
     CDS             complement(340..669)
                     /locus_tag="ACHUPB_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0760889.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="ACHUPB_004244"
     CDS             complement(674..859)
                     /locus_tag="ACHUPB_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0760890.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="ACHUPB_004245"
     CDS             complement(856..3495)
                     /locus_tag="ACHUPB_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0760891.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT          852 a          956 c          801 g          911 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg
//
LOCUS       DAZOZD010000343         3484 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOZD010000343 DAZOZD010000000
VERSION     DAZOZD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.347"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..287
                     /locus_tag="ACHUPB_004246"
                     /pseudo
     CDS             1..287
                     /locus_tag="ACHUPB_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(317..3229)
                     /locus_tag="ACHUPB_004247"
     CDS             complement(317..3229)
                     /locus_tag="ACHUPB_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR0760892.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3286..>3484)
                     /locus_tag="ACHUPB_004248"
     CDS             complement(3286..>3484)
                     /locus_tag="ACHUPB_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760893.1"
                     /translation="GDMFTSEQHRCSNEKKEFQRRVYLRGDAFINLYTNFYSGIMTGL
                     QLLRHFQRIALPAYHPTFFIP"
BASE COUNT          869 a          937 c          852 g          826 t
ORIGIN      
        1 gttccgtcag cactgcgaca gcgttcttta cccggtcaaa acagcgttgc ggtgcaccac
       61 gcctgacgga tgaactgcgt gctcagggtt accactttaa cgtaaaaacc gtggcggcaa
      121 gcctgcgccg tcaggggctg agggcaaagg cctcccggaa gttcagcccg gtcagttacc
      181 gcgcacatgg cctgcccgtg tcagaaaatc tgctggagca ggatttttac gccagtggtc
      241 cgaaccagaa gtgggcggga gacatcacgt acttacgtac agatgaagtt cggttacatc
      301 ctgtcagcac agaacctcac gctttttgag cggcacgacg gatccccccc acccagtatc
      361 cgacagactt ttcactgcgt tcgtaaaaac ccttcttccc tcccagcata tccactaatt
      421 ccggtgacat atcctgcaga taagtctgca aatcatgccc cagatcttcc ggtgactgca
      481 actcataaac cgcgcgccac acatcaccct gccttatttt aagccccggg ttaagaatat
      541 actgtccgtg gttcagcctc agaaaaagct tttttccata aggatttttg ctgttaactt
      601 catgttgaga gagctggctg ctgatataac tccttcgttt gcggcgttcc ggcagcaacg
      661 cttcaggaag atgaaccaca cactctgcca gtcgtgccgc actgaaggct tccgaaaaaa
      721 acagagcact tcccagatgc gtgtggaaca gcgcctgcat caggatcacc agaaacattg
      781 ccatctgccg gttgtccagt ttgatgaggc gtccttccac ctgcagtgac agactttcag
      841 gtttcagcag ggtatacaac tgcgcacttt tctgttgcgc ataacggggc gtactgaccg
      901 ccgctgacag aacttgatga taagccgcca gcccgtcatt accggtcagc aagggatctg
      961 caccacgctc cagcagtaac tgaaccagtg caatattacc tgcccgggct gctgacatca
     1021 gcggagtaag attaaacggc gaacggtgat caacaccata acgttcaata tcccgcaaca
     1081 cggcaaccgg gttattcatt gagtatggcc ggaaatactt cagctccagt aactgcagag
     1141 tcttcgttct gggctgcctt gtgggcttaa aatcttcccg gtacagtgca ttatacagcg
     1201 ggaacaggga ataaatcaga ctgtactcca gcgcctgcag ttgtatggtt ttatcggctg
     1261 tacctgcgag gatatgctgt ttccactgtc gtgcatcctc tgcggtgata atctgccatg
     1321 gcatctcaga tgtctgcagg atccggctac ggatggtgtc cgcctgctcc tgcttcccct
     1381 gtttttcaag cagatgtgcg gttttctgcc actcatcacg gctggaaatc tcttcctgca
     1441 gattcagagg ctcttcctga tgcgtgagcg caagcagact ccacagagga tgctccacct
     1501 gctgttcaac aagatacaca ttgtgtgtag cacgggtgag cgcaacatac agcgcgttgg
     1561 taaaaaattt atagatttcc gctgaacgat cctgcctgtc ccgcggacgg gaataacggg
     1621 cctcgccctc aagatcagcc ggtgtaagac cctcgcaaat atcatcaaat gcctgccggg
     1681 cagcgcttac tatattgtaa aggatcaccg tttcgtattc cagcccctta acctcctgaa
     1741 ccgaaaagac cagcggcgta ctgaaccagc atcgcgcctg tgctttctgt tccggatgca
     1801 tcacaatgac cgctacacga ttcgaaagtg aggttttggc attcagttcc tgctttgtct
     1861 cttccctgtc gtccagcaac cggatggtcc cttctgcctg accgcagctt ctgacaaaat
     1921 ggtgactttc ccgatcaata gcactaaagc ggacctgttt gagtctcagc aaccggttag
     1981 ccagtgcagt gacgtggtgc ccgttacggt aattcgcctg caggatatta acggttgtgt
     2041 cgtttccctg ttgctgccgg aaaaagagac ttttgagcga actccatgag aaaaagctcg
     2101 gatgaactat ctgattggca tcaccacaga gtaaaaactg accgggatga cgtagcgttt
     2161 tcagtaccag ttgcagttga acaggcgtca tatcctgaac ttcatcaaca aaaatgacat
     2221 cataacgggg agttgccaac gaaagatact gatgactgag cagattactg tcatactgat
     2281 gcgactgttt cagccatgcg atataacgtt caaacaggac atagaccgtc gggcggtcct
     2341 ccatgccata gagcgactgc cgtatcccca gtgacagata cgcctcccgg gacagcggcc
     2401 cattcccgga ggcaaccgca ccgatcacgc ccctgaactc ctcatacagg gtatgggcag
     2461 cccccagtgc ggcgatagcc ctgttgcggg gcagccagtc gctgaacgca ctgaaagtca
     2521 cctccctgcc tttcggaatg cgcagagttt ccagaaattc cgtcagcgaa agaaagtcaa
     2581 tattctgtac ctcactccct tcccctgaag cgtcatagag agtacgcgct ttttccacca
     2641 gaaatgagga aaggctgaga tacagtatat caccagcagc ctgcttcatt ttttccagta
     2701 ccagcgccgt cttaccactt cccgccgagc caacaatcac cagcggtagc gggtgttcgt
     2761 acagtgcctg ctgatcatca tcaaaggaca gcattttatc caggcggtgg aaacgcccat
     2821 gagacggatt gatatacgtc agcgcttcag tcgcgatatc aacaggatcc ggcactggtt
     2881 gttgttgtaa gcgcccttca tcaatcacca cgtttctgcg aagaaaacgt gaagtgttat
     2941 aggcatgatt gcgaatatat tccagaacaa ggcagatagt ttcaccgtgg tagcgataca
     3001 gcgaaaacag taatcggtcc cggatactga gccgggcgcg gtaaagattg gtgtcaattt
     3061 tacgaacatc agcctgaaca aagttatcgg tggcaatagc ctgtgcaaat tttttataac
     3121 ccgggatcgt gtcaggattc agagtatgat aaagacagat tttcatggct gccggtttcc
     3181 gtcgtgcaaa aaacatgctg attttacgaa ggaatgcctt actgaacaac cttgcccgga
     3241 ataccccccg aaatcagatg gaacaatcag aaaaatgctc tgcagttatg gaataaagaa
     3301 ggtggggtgg tacgccggga gggcgatccg ctggaaatgg cgcagtagct gcaggcctgt
     3361 catgatgccg gaatagaagt tcgtgtacag gttaatgaaa gcgtcacccc gtaaataaac
     3421 tctgcgctga aattcctttt tttcattgga gcacctgtgt tgttctgagg tgaacatatc
     3481 acct
//
LOCUS       DAZOZD010000344         3466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOZD010000344 DAZOZD010000000
VERSION     DAZOZD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.348"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1280)
                     /locus_tag="ACHUPB_004249"
     CDS             complement(<1..1280)
                     /locus_tag="ACHUPB_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR0760894.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSDIMGLNKIHNISICNDGLDYGINIDNHFY
                     IRNHIISRPSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHAIHTRTQ
                     LENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRYYQAVFL
                     ALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLCALIRRY
                     FKESENKFINHAWQTLIRTLLNNSRTEQPNYDFK"
     gene            complement(1565..1897)
                     /gene="pemK-mt"
                     /locus_tag="ACHUPB_004250"
     CDS             complement(1565..1897)
                     /gene="pemK-mt"
                     /locus_tag="ACHUPB_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR0760895.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1899..2156)
                     /gene="pemI"
                     /locus_tag="ACHUPB_004251"
     CDS             complement(1899..2156)
                     /gene="pemI"
                     /locus_tag="ACHUPB_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR0760896.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(2249..2902)
                     /locus_tag="ACHUPB_004252"
     CDS             complement(2249..2902)
                     /locus_tag="ACHUPB_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR0760897.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3000..3140)
                     /locus_tag="ACHUPB_004253"
     CDS             complement(3000..3140)
                     /locus_tag="ACHUPB_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760898.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
BASE COUNT          948 a          813 c          730 g          975 t
ORIGIN      
        1 tgtttaaaat cataatttgg ttgttctgtt cttgagttat taagtagagt tcttatcagt
       61 gtttgccaag catgattgat aaatttattt tctgattctt taaaatatcg tctgattaaa
      121 gcacataaat cttcgacact tttctgacga gcgctagctg cccaacggcc cccttcggtt
      181 tgcaccattg aacgttgtac actgtctcct aactgagcga taaatttctg ttcatcgtca
      241 agttgcatgt tttcatttat gattaattca tacaaagcaa gaaaaacagc ttgatagtaa
      301 cgaggacatt cttgagatga agatgcattg cccagaatgt gtgatctaaa gttcaaatta
      361 tttgcatcaa ataatttttg aatcatctca taaacgaaga ggtaattatt gacaatttta
      421 tctgcaccat ttgtctgaat gtaattctct aattgagttc gtgtatggat tgcatgactc
      481 gtcgaacctt caccgtaaaa tgtatcgagc gatgttgatc ctgatgttgg cttatcatct
      541 aaaaatatat agcccaagat atttgcaact aactcttcat cgtcagagtc tctaatgcta
      601 ggcctactaa tgatatgatt tctaatatag aaatgattgt ctatatttat tccataatcg
      661 agtccatcat tgcaaattga tatgttatgt attttattca gtcccattat atcgctatgt
      721 gtagtatctc ctcttataat tgtcgatatt ttccttacta actctgaaaa attactaacg
      781 caccccgcac ttcgtagttc ttgttttgat aaaattttac cattggaatt tatgcgtcga
      841 aacacttcgt caatgtggtc ctctgttgaa gagtattcag atatagcaaa tctatagcga
      901 gcaattaatg tagaactttc gcgggatagc actggctctt tttgttgaag tttaccactg
      961 tcttttagtt gttttgttaa tgcaatggca tctaaattaa agtatttacc atttaatgaa
     1021 aatcgctgct caataaaact cattaaagct tctagtcttt gcattccatc aaggatttct
     1081 cttttgttgt caatggaaac aaacaatagc aaagggatag ggtattgctg agtcattgat
     1141 gaaatcagtg ctgttttctc ttcgagggac catactaatt ttctttggta tcttcgattt
     1201 ataattaatt tatcatccag ataccactcg tataattctt ctgctgtctt ggagtcaata
     1261 tctaagtttt cattagccat tagatgtatc tcgagtattt agttaagaaa ctgataggtc
     1321 agtatttgca tcaatgttca ttgaatttgt atccgaagtc aatctgatgt aggaacttca
     1381 aaactgcgca aaacacgcaa atacgcacaa gaaagtaggg ctgcgcttgg aattcggaaa
     1441 ataaaccaca tcaaaactac tttttagcca aataacgttt gggaatcacc agaatggtgg
     1501 gacaacagcg gttttagtgc cctaaatcgt acgttttcat ccagttgccc ctcaaacccc
     1561 atgttcaagt cagaatagtg gacaggcggc caagaacttc gttcatgata gtctccggaa
     1621 cccgttcgag tcgttttccg ccccgtgctt tcatatcaat tgtccggggt tgatcgcaac
     1681 gtacaacacc tgtggtacgt atgccaacac catccaacga caccgcaaag ccggcagtgc
     1741 gggcaaaatt gcctccgctg gttacgggca caacaacagg caggcgggtc acgcgattaa
     1801 aggccgccgg tgtgacaatc agcaccggcc gcgttccctg ctgctcatga cctgcggtag
     1861 gatcaagcga gacaagccag atttcccctc tttccatgtc agatttcctc ctgaccagtc
     1921 gccggtgcat ccagccattc tcgttcttca gctgatattt cagcattcgg atcacactgt
     1981 gccagtagct cagccagtga atattgcggg cgtctgtacg gctcaacaat cagccggcca
     2041 ttatcaatga ccatgccaac ttcattatct gtgcccagag acagcgcatt cagcagtgcc
     2101 ggtgggacgg tcagcataac tgagccgcca accctcttca gtcgggtggt atgcattctt
     2161 cacctccata aaagttatat ttaaatataa catccactaa aaaaacacac caggctttaa
     2221 cgcacaatgt ttaataaaaa tataactttc aaccaaacag taaacccagc gtggttgctt
     2281 ccatatgcag caataccggc agcagcaggc caccacttct gatcctggcc actgatgtaa
     2341 tcaaccccac caggaacagt tctgccagtg tcagcaggtt ctgatactgg ctgtgcgcgg
     2401 cgacgaacaa caacgacgtt atcagcgccc ccagccacat cgtccagcag taccgtgaac
     2461 ggaagacgtt cagcataatc ccccggaaca gcgtttcctc attcaacggg gcaaggataa
     2521 agatggtcag caacgtcagg atcacgtcag gtatggactt atcggcaaaa agtttcgtca
     2581 taaatggctc agcaggcaga gccagcgcct taccgagcag aaatacaccg acatacacca
     2641 cggccatcgc accgaccagc cacggtacgc caacgttgcg cagctgacca acgaccggta
     2701 gcggagctat ccaacggcgg tataccagga aaacacacag caggtacatc agaacagtac
     2761 catgactgaa gaacaaatag ttttttcctg atccataaag cagaacggcc tgctccatga
     2821 caaatctggc tccccaacta atgccccagg cagccagcat aaccagcata aactgcagat
     2881 attgattacg tgtttgaatc attgcatcgc ctgtaaattt ttaacttgtc ctatttttgt
     2941 cattaccacg tatatacaca tgtataacaa ttcagatatc gttaccagga tatgccgcat
     3001 cagcggcatg gaaggcggca ctctgttgtt tcatatgata caggagtaaa accgccgaag
     3061 cccggcgtaa gccggtactg attgatagat ttcaccttac ccatccccag ccctgccaga
     3121 ccatacccgc tttcagccat gagagagctt ctgtgcgcgg tcggagtggt cccgacgagg
     3181 gtttacccga agtcggggcg tatctccgcg ttagcgggcc gtgagggccg cttacgagcg
     3241 tgtactgaga acttccagcg agaagactga cagcgatgaa gatgtagtta caacattcat
     3301 aattaaaagc gactctgttc cggccctttg ggccggggcg gggccgcttt tcagttatga
     3361 gggaggggct ttgtggtttc ggttctgcgc tggaccgggg tttttctgga ggttgttttt
     3421 gtgtgttgta actaaagtgg ctccggtcgg ggcccgccgc ttgcgg
//
LOCUS       DAZOZD010000345         3412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOZD010000345 DAZOZD010000000
VERSION     DAZOZD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.350"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..470
                     /locus_tag="ACHUPB_004254"
     CDS             <1..470
                     /locus_tag="ACHUPB_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0760899.1"
                     /translation="DDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSE
                     AAKEVVKKEDKISDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIK
                     ERFRNAKLKNKVSDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            470..766
                     /locus_tag="ACHUPB_004255"
     CDS             470..766
                     /locus_tag="ACHUPB_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760900.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            1090..1380
                     /locus_tag="ACHUPB_004256"
     CDS             1090..1380
                     /locus_tag="ACHUPB_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760901.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            1601..1816
                     /locus_tag="ACHUPB_004257"
     CDS             1601..1816
                     /locus_tag="ACHUPB_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760902.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            1963..2268
                     /locus_tag="ACHUPB_004258"
     CDS             1963..2268
                     /locus_tag="ACHUPB_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760903.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            2744..>3412
                     /locus_tag="ACHUPB_004259"
     CDS             2744..>3412
                     /locus_tag="ACHUPB_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0760904.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIE"
BASE COUNT         1135 a          610 c          724 g          943 t
ORIGIN      
        1 aagatgactt tgaagaagaa tattttaaga agggagcttc gctctgtgat tcacatgaat
       61 gtgattctct tggtattgaa gaggctatta aagagactgt taaaacatct gacttagatg
      121 accttgatag tgaagctgct aaagaggtcg ttaaaaagga agataaaatc tctgatgtga
      181 ttgctagtag tgaccataaa aacacacagt gccccgccac tgatgatgaa ttaccggatg
      241 ttgaccttga tgatgaagaa gaccctgaag aggaattgaa acgtgaacag cgtaaagcag
      301 aaattaaaga aagattcaga aatgcgaagc taaagaacaa ggttagtgac ctcgatgact
      361 ttgatgatag tgacgatgat tttactttta caccaaaaaa gaaaactcac accgagccaa
      421 agaaagaaaa agttaaagtc agacgtgttt atggtggagg aatgttttaa tgaaaactta
      481 taatggcgag agaaaatacc gtgactattt actcagacgt tacagcattt ctcgtgaagg
      541 ttacttgatg aaagatactc acggggaagt ttaccggatt cgaccgaaga aagaagggag
      601 ggattattat ttcgtcgatg atgtgacaga tttaaagatt gacgctttga agtttgcggt
      661 gctgtatcat tacgatttat gggattcagt ccaccagcta cgattaaaag acggtgaccc
      721 cggtaatctg agggccacca atattatcaa gggaaagtgc cggtaaggcc accagctatt
      781 tatttactga aatctctatc tcaacacgaa gaagtttaag tcgaattgta agctttgtct
      841 tttttgtaat gactcgattc ataacgcaaa acaccaatag cacaataaat accgcaaagc
      901 ccaacgagct aaaacgatga gattcaataa ttgctgtgaa aaaatcatgc attaattttc
      961 cttttgtgaa aggaaacagc acctgtatca gcccctacaa ccatagtatc atgtaaatga
     1021 ggtactgaca atatgttaaa tagatgttaa gattgtagta tgagtaagag atttaaggag
     1081 atttttacaa tgactcacac agttacgcgc cttttgccag aggcatttga acgactggca
     1141 gcaatcaaag ccaaatatcc cgatatgaag cacacagcgc ttgttgggga gattttaaaa
     1201 gagtcggtaa accttagccc tttcgataac gttaagggcg tcgcagaggg ttctaaggcc
     1261 atcagaatca caacgggcca actggaacta ctcaaagcac agtccgcagt agcaggttgc
     1321 tcaatgattc aggtgttaag cgctaaggtc catcaactgg cggatagatt ggaggtttaa
     1381 tgtgttatag gttccaatat tccacacttg gccgccacct aatcagacag aatttagcag
     1441 atgctgaatt tgaagatgtg accctcaaag caaacaaacg attaaaaccc tctcaaatcc
     1501 accagcttaa acacgaatta aagccactgg taatactcga ctataacgac cctgaacagc
     1561 ttgagcaggg aattaagctt atttctgaat ggggtgcttg atgaaaacta tcacagtaca
     1621 acttcagact aataaagcat ttcgttattt tgaaaacttg cttgagttat acgaaggatg
     1681 gggaagtatc cacggcaaag atgatatcta tcttcacttg tcagccccta actattctct
     1741 gaagactccg gttaagcaaa gctggttaaa agactacggt caccagatgg ggctattagt
     1801 ctcagacttg agctaaaaac agcaaaacat atctctcagg ctttaccaga cgcaccagaa
     1861 acgtttttaa gcccctaatc agggtaactt agggaatcgc cttaagtggt ctaaaaaagg
     1921 ggttaaaacg tcttagaatg cgatacagga aggattttaa aaatgaatta cccaatgcaa
     1981 attatggttg accaagaaac catgaattgg ttgattgacc tgaacaggtc gggctttaat
     2041 atctatttca tgcattgttt tgaagcgtca acgtatattg acaagcatat aatcaacgaa
     2101 attaaacttg gtgactatgt tcacaagctg gaagtaacag acccatacaa taagcgcttt
     2161 atcattgaag agtgcaatcg acttgattta agccctgagc aattattgaa gctgaatgta
     2221 ttcttgacct atcagaatga attaaaccct tacagcgagg tgatgtaatg acactcgtta
     2281 ttctctggat gactttaggg ttatttgttg gttgctgggt aggtaatacg gtaaataaga
     2341 attgatgtta ccaatctgtt aaaaagctgt tgacaaataa ccttaagcat gagatgatgg
     2401 taatagggaa gagcaactct tcctgaatta aactattaat cattctacat caatgatagt
     2461 tattctgaaa ggtgcaatgc ttttcgctac atgacctttc aggttagtct gagaggtcat
     2521 ctcgaaaagt actcttgttc attcgtttga atcctttttg aatcctttag atgttttaat
     2581 ttcttcacct tatgaatggc cctttataag aaattatcta gggctattca gcttttaaaa
     2641 agcatacttg gcaaaccgcc ttttaagtct cttctaagtt tagataggct gtcaggtgtg
     2701 ctttttaaaa actgaaacac ttccgcttag aagaggaaaa gcaatgacaa atgatattta
     2761 cattaatggc aaagtgattg atagcttcag tgactggtcg ccacagactg aacatccagc
     2821 tatcactatt cagcgtaaag agcatgacaa gcgtattgag cttgagcaat cgctaaagat
     2881 gtcaccaaaa acaaccatct ttgtgtctcc tgagcctcaa gagatgcctg atatttgcaa
     2941 gagtgaagca ttacttgagc ttgaaaagaa atattaccaa ctgctgaaag aacagcgcct
     3001 tagattagat gacgcttaca gcaaagttac cttgatgcaa tcagctattg agccgtcaga
     3061 atttgagatt caggatgaat tgtcacagaa accatttgtc tattaccaat acgaagataa
     3121 tgatggtttt ggtacttttc aagaaaacgt cgcagccgtg attagtagtc ttcctgctgg
     3181 acatcgaatt gtgaagatgg ttaaggcatc aagaggtgct ggtcagttta tttacatgac
     3241 taataagagc gatgaagaac tcgctgaaat tgctagacag aacatcttgg catcacgtaa
     3301 caagcaactt gataatgtta agttgtatct ctcaagagag cttcaggcta tgaaagagct
     3361 tgtgaaagaa tatgaagatg ctaagaagat tgctatgcag gctgatattg ag
//
LOCUS       DAZOZD010000346         3367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOZD010000346 DAZOZD010000000
VERSION     DAZOZD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.351"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUPB_004260"
     CDS             complement(<1..312)
                     /locus_tag="ACHUPB_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR0760905.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUPB_004261"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUPB_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0760906.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUPB_004262"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUPB_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0760907.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          917 a          874 c          731 g          845 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgac
//
LOCUS       DAZOZD010000347         3322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOZD010000347 DAZOZD010000000
VERSION     DAZOZD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.352"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(33..167)
                     /locus_tag="ACHUPB_004263"
     CDS             complement(33..167)
                     /locus_tag="ACHUPB_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760908.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            243..2804
                     /gene="mutS"
                     /locus_tag="ACHUPB_004264"
     CDS             243..2804
                     /gene="mutS"
                     /locus_tag="ACHUPB_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0760909.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2910..3322
                     /locus_tag="ACHUPB_004265"
                     /pseudo
     CDS             2910..3322
                     /locus_tag="ACHUPB_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT          825 a          868 c          897 g          732 t
ORIGIN      
        1 tgaaagggga ctatggctct gaaggaggag gtttattcaa ccgacgcaat tttgcgtact
       61 tgcttcataa gcatcacgca aaagctgcaa aacagcatct ttcccggaac cagcatcaag
      121 aactcgccgt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg cgccttatgt
      181 gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc ggacataacc
      241 ccatgagtgc aatagaaaat ttcgacgccc atacgcccat gatgcagcag tatctcaggc
      301 tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt tatgaactgt
      361 tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc aaacgcggtg
      421 cttcggcggg agagccgatc ccgatggcgg ggattcccta ccatgcggtg gaaaactatc
      481 tcgccaaact ggtgaatcag ggagagtccg ttgccatctg cgaacaaatt ggcgatccgg
      541 cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca ggcaccatca
      601 gcgatgaagc cctgttacag gagcgtcagg acaacctgct ggcggctatc tggcaggaca
      661 gcaaaggttt cggctacgcg acgctggata tcagctccgg gcgttttcgc ctgagcgaac
      721 cggccgaccg cgaaacgatg gcggcagagc tgcaacgcac taatccggcg gaactgctgt
      781 atgcagaaga tttcgccgag atgtcgctga ttgaaggccg tcgcggcctg cgccgtcgcc
      841 cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa tttggcaccc
      901 gcgatctggt cggttttggc gtcgagaacg cgccgcgcgg actttgtgct gccggttgtc
      961 tgttgcagta tgcgaaagat acccaacgca cgaccctgcc gcatattcgt tctatcacca
     1021 tggaacgtga gcaggacagc atcattatgg atgccgcgac acgtcgtaac ctggaaatta
     1081 ctcagaacct ggcgggcggt gcggaaaata cgctggcttc tgtgctcgac tgcaccgtca
     1141 cgccgatggg tagtcgtatg ctgaaacgct ggctgcatat gccagtgcgc gatacccgcg
     1201 tgttgcttga gcgccagcaa actattggcg cattgcagga tttcaccgcc gagttgcagc
     1261 cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct ttacgaactg
     1321 ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg gagctgcgtg
     1381 cgcagttaga aactgtcgat agtgcaccgg tacaggcgct acgtgagaag atgggcgagt
     1441 ttgccgagct gcgcgatctg ctggagcgag caatcatcga cacaccgccg gtgctggtac
     1501 gcgacggtgg tgttatcgca tcgggctata acgaagagct ggatgagtgg cgcgcgctgg
     1561 ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa cgtaccggcc
     1621 tggacacgct gaaagttggc tttaatgcgg tgcacggcta ctacattcaa atcagccgtg
     1681 ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa aacgccgagc
     1741 gctacatcat tccagagcta aaagagtacg aagataaagt tctcacctca aaaggcaaag
     1801 cactggcact ggaaaaacag ctttatgaag agttgttcga cctgctgttg ccgcatctgg
     1861 aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtg aacctggcgg
     1921 aacgggccta taccctgaac tacacctgcc cgaccttcat tgataaaccg ggcattcgca
     1981 ttaccgaagg tcgccatccg gtagttgaac aagtactgaa tgagccattt atcgctaacc
     2041 cgctgaatct gtcgccgcag cgccgtatgt tgatcatcac cggtccgaac atgggcggta
     2101 aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc ggcagctatg
     2161 taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc gtaggcgcgg
     2221 cagatgacct ggcttccggg cgctcaacct ttatggtgga gatgactgaa accgccaata
     2281 ttttacataa cgccaccgaa tacagtctgg tgttaatgga tgagatcggg cgtggaacgt
     2341 ccacctacga tggtctgtcg ctggcgtggg cgtgcgcaga aaatctggcg aataagatta
     2401 aggcattgac gctgtttgcc acccactatt tcgagctgac ccagctaccg gagaaaatgg
     2461 aaggcgtcgc caacgtacat ctcgatgcgc tggagcacgg cgacactatc gcctttatgc
     2521 acagcgtgca ggacggcgcg gcgagcaaaa gctacggtct ggcggttgct gctctggcgg
     2581 gcgtaccgaa agaggttatt aagcgcgcac ggcaaaaact gcgtgagctg gaaagcattt
     2641 cgccgaacgc cgccgcgacg caagtggatg gtacgcagat gtctttgctg tcagtgccag
     2701 aagaaacttc gcctgcggtc gaagcactgg aaaatctcga cccggattca ctgactccgc
     2761 gtcaggcgct ggagtggatt tatcgcttga agagtctggt gtaataacaa ttcccgatag
     2821 tcttttgcta tcgggaatat taacgacaac tgacgaatca aataaaaata ccctgtataa
     2881 taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa aaaatcaatg
     2941 cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat cagttattac
     3001 aatcccgtta acatcaactc tctttttacc ctgaaaccga cttacttatt tccacaggcg
     3061 ataatattga tcgtgggccg aagagtctta acgtgctgcg cctgctaaac caaccctggt
     3121 ttacgtcggt taaaggcaac cacgaagcga tggcgttgga tgcattcgaa actggcgatg
     3181 gaaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat tcagagcaac
     3241 aagaggcaat agatctgttg ctgaaattcc atcaccttcc acatattatt gaaatcatta
     3301 atgacaacat aaaatatgcc at
//
LOCUS       DAZOZD010000348         3319 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOZD010000348 DAZOZD010000000
VERSION     DAZOZD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.353"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(108..1472)
                     /locus_tag="ACHUPB_004266"
                     /pseudo
     CDS             complement(108..1472)
                     /locus_tag="ACHUPB_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1537..1656)
                     /locus_tag="ACHUPB_004267"
     CDS             complement(1537..1656)
                     /locus_tag="ACHUPB_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0760910.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1880..2038)
                     /locus_tag="ACHUPB_004268"
                     /pseudo
     CDS             complement(1880..2038)
                     /locus_tag="ACHUPB_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2383..>3319)
                     /locus_tag="ACHUPB_004269"
     CDS             complement(2383..>3319)
                     /locus_tag="ACHUPB_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760911.1"
                     /translation="TRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQVKGHYGDK
                     FKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNGNCSERLF
                     NFYECCYKFLPRAQPQDKVESYNSALQAFSIFRSSTLNNNDVGFNFKLFPEVKLSGEN
                     LETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNYDIQYVNW
                     RTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDIL
                     FMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          916 a          754 c          611 g         1038 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctagc aactaaagca
      121 ctgcccgtct cctggttaac cccatgtgct ataccgccgg tatagacaat cagcgagcct
      181 ccggcgctaa tattgctgcc agttgccgta ccatctttct caacaacctg ccggctcccg
      241 gtggaaatat ttgtcgtgtc agctttcccg ccgctcttga tattttgtgt tccgctgttg
      301 atattggtac ccgtggcaat accatgatta ttaatattct gtgtaccacc attgattatg
      361 gtattggtcg cgtttctggt aacatccatt accccgccgt tatctattcg ggtcgcatca
      421 gctttagcat tcgttaaaac tgacattgtt cctttatctt taataatcgt cttatttgcc
      481 gaaccatatg cgtttatgtc taaatgacca ccgttttcca gcaacacatt gtctgccacg
      541 tgattgtgga tggagaatgc accttcacta ttcgtaccgc tcaccgtcgt accgttagtg
      601 tttgttttta aaattgcacc atcgtgctgg gtaacatttg ttgccgtacc accactaaca
      661 tcaagcacgc caccggaata aacttcaata acatccgagg agctggtgta atcaacaatt
      721 tgcgtaccac cagaatagat ctgagtattt tttgccgttg acttattatt cagagactga
      781 gttccacctt caatcgtcgt gtccagcgca tggctttcat atactctttg ctcaccgcca
      841 tttttaatgg tggttgttcc tactgtgctc tgttcaacat actgtcgtcc accatttatg
      901 attgtgttcg ttgccatact tccttgcgtg atgtcctgat aaccggactt atttatcgtt
      961 gtaccatcaa catgcccctg agttgttact gtctgccctc caccatcaat gatggttcca
     1021 ttcgcagtcc ccccacttat gtgtgaatta ccacccttaa ttgtcgttcc attgctgata
     1081 ccgcctgtat aaacgtcctg attgccactg tcgattatcg taccggtgga aatgcccccg
     1141 tcatgaattg tctgtcttcc tccgttaatg gttgtattat tagactgccc catataattg
     1201 ttattccttc caacatcttg atatccacca gattcaataa gacttccatt agatactccg
     1261 ccataaacac tctgctggcc atggttgata atatgagtgt tatttgttgt acctcgttca
     1321 tctactcgtt ggttgccatc tacagtctca tcgtttacca caccaataac attagtagta
     1381 aaggccgcca tcacgggcgg ggcatatatc aaggcagata tcaataagga aagtactgag
     1441 cggcgacaat aatggggact ggtcctgttc ataaatttca tcctctgaaa agtgaatact
     1501 gagtagcgtt taagcgactt tagctctgct gcaacatcag cccgcaggca ccagaccggg
     1561 gggttcatcc tgaagagaca gcgcaagtgt attgtgttca ccgctcatca aagacatcat
     1621 gatgaaatga tgatgttcca cataagaaag aggcattttt taaacacagt gcgctgaagt
     1681 gtgattggat aaaaaagtca atctattcag gaaatacggg cgtattcttt tctttcgaca
     1741 atcaggccgt cggcaaaata aaatgattta cataatcgtt tctgatgaat atcttctgct
     1801 cacataaaaa tcacacaata actttgagat cgcagattct tttactttta cagcattcgt
     1861 ccccccattg ttgggcaaat atagattggg ccagagcacg aaagttaata ccacgtttgc
     1921 acagctcctc caacagcacg acaagatgcc acatactgcg ccccagtcgg tgcggtttac
     1981 agactacccg tgcgtccacc gccgataatg tcctgagcag ttttttcagt ccggacctcc
     2041 tgccacggtt ttacgttgaa gaggtaaccc tgagcacgca gttcttcagt caggcgtggt
     2101 gcaccgtaac gctgttattg ctgggtaaga tcaaaaaact ttcaggcagc taaggaaagt
     2161 tgaaccagac attagatgaa atatttcaac caattacagc accaatttaa actcagtaaa
     2221 tcacagcaca gatcaagggg ttacgtgaaa gcgtagcccc tttttctttg gtagtggcag
     2281 caaaatggtt gtagtgtaaa aaataatccc gtttaatcaa tcaataatac atattgtttc
     2341 aatctacgtt attatctctt tgtaaaaata gccatttatt aatcattgaa aactgctttt
     2401 agaacttgat acaacgggac tagtcacaac aggactattc tcaacgggat catcctcaga
     2461 ggaactatca tcaaagtcat catccataaa taaaatatca tcgaatggtg ccacgcccgt
     2521 gatgagtttt attttattat tacgatcagt caagactcca cttaaaccgt tttcgctcac
     2581 aggttttaat gatttttcat tactcttgtt gtaagcaggc gcattaaaaa tacacggagt
     2641 atcaagatca aacaatgacg ttctccagtt cacatattga atatcatagt tactgaagtt
     2701 ctgtccagaa aagaagcatc ccttaaaatc caatccacct aaattatata aaccatcctc
     2761 ttctttttgg agagtaatgt taattctggc tatctcccgg acaaaacttc cttttttgta
     2821 tttgaatacc gtttcaagat tttcaccaga cagcttgact tctgggaata atttaaaatt
     2881 aaaccctaca tcattattat tcaaagtaga tgaacgaaag atggaaaaag cttgtagtgc
     2941 tgaattatag ctttcgactt tatcttgagg ctgcgctctt ggtaaaaact tatagcagca
     3001 ctcataaaaa ttgaataaac gttctgaaca attcccattt ttatcaatta ataccccccg
     3061 gaattctttc acaaaggcat ccgttatttt ttgaatatct atggtgtgtt cctttgactc
     3121 tgacaaagat ggagtctgtt gaagtgcatt gctcatctgg ttaaggaaca tctgcatttc
     3181 tttaaattta tctccatagt ggcctttaac ttgctgagag tttttagtta caggtgaaac
     3241 cttatctatt tggttgtcat ataaattatg tggttccata cagacatttg gttgtgtttc
     3301 taaaataaca ctacgtgta
//
LOCUS       DAZOZD010000349         3295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOZD010000349 DAZOZD010000000
VERSION     DAZOZD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.354"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(293..368)
                     /locus_tag="ACHUPB_004270"
     tRNA            complement(293..368)
                     /locus_tag="ACHUPB_004270"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Asn,seq:gtt)
     gene            complement(469..1956)
                     /gene="yeeO"
                     /locus_tag="ACHUPB_004271"
     CDS             complement(469..1956)
                     /gene="yeeO"
                     /locus_tag="ACHUPB_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0760912.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2117..2192
                     /locus_tag="ACHUPB_004272"
     tRNA            2117..2192
                     /locus_tag="ACHUPB_004272"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2150..2152,aa:Asn,seq:gtt)
     gene            complement(2230..3180)
                     /gene="cbl"
                     /locus_tag="ACHUPB_004273"
     CDS             complement(2230..3180)
                     /gene="cbl"
                     /locus_tag="ACHUPB_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0760913.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          966 a          859 c          696 g          774 t
ORIGIN      
        1 gcaaagtggc aattacgcat agtttcccga taaagacgcg atagcgacat cccgcataag
       61 gcatttttct ctttatcttt gtacggtact tcatggaaca gagtttttga ccttgcgaat
      121 cgtgatgtct gttggggagg ggcaatttgc tcactgaagc gtgagactcg attaagcgca
      181 cgaaacacag aaatcaaaaa acccggtcac ttttttacaa ggtaaccggg taaaaataat
      241 ttttattttt taactgtttt gagactcata gagatgtctc aaaactaaaa tttggctcct
      301 ctgactggac tcgaaccagt gacatacgga ttaacagtcc gccgttctac cgactgaact
      361 acagaggaat cgtgagaacg aggcgaatat tagcgatgcc cacccacaat gtcaaagcct
      421 gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt attccgacac
      481 aactggcttt ttttcacact tttgcggttc ggatcgaggg tatttccaca gccaacgtcc
      541 agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa acattcccat
      601 ccagacacca accacacccc agccaagcat gatgcccagc acataaccga cgacaacccg
      661 gcaaccccac atgctcaaca tagaaaccca catggcgtaa cgggcatcgc gagcaccttt
      721 aaatccagcg ggtagcaccc atgaggcgga ccaaataggc ataaataaag catttagcca
      781 aatcagaatc acaacgacat gtttaacctg tggatcctgg gtgtaaaacg atgccataac
      841 cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg aaagccagaa
      901 cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc ttcggcctgt
      961 aatgatcgta gaagcagagc cgagcgcact tccgggtaag ttgataagag ccgcaattga
     1021 aaacgcgata aaatttccgg caataacact ggtccccatc ccggcaacga acatttgggt
     1081 taataaccga ccactggtaa ataacactga ttcgacactc gcgggaatac caatccccat
     1141 gacttcccag ataatgctaa aattcagcgg tttaaaatag ctctttaacg aaatccttag
     1201 cgcaggatta aaaccaatcg ccagcaccca caaaattgca actgcgccaa tataacgaga
     1261 aatggttaaa cccagccctg ccccgacaaa tcccagtccc ggccaggaga aaaggccgta
     1321 aatcaatatg ccgctaataa taatattaag aatattcagg ctaccgttaa tcaatagcgg
     1381 tattttcgta ttccctgcac cacgaagtgc cccgctacca ataagagtga tggcagctgc
     1441 tggataactg agtaccgtca gctccagata agtcaacgcc agtgctttaa cttctgtcgt
     1501 ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa tgatgaataa gcgttgccaa
     1561 cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg
     1621 tcgatcccgc ttaccgagac taaatgccac aacgacagta gtaccaagat cgatagcagc
     1681 aaaaaaagcc ataatgacca tattgaagct gtccgccaat cccacgccgg ccatcgcatc
     1741 ttttcccagc cagctgacca gaaaagtgct cagaaccccc atcaacagga cacaggcatt
     1801 ctccatgaag ataggaacag caagcggggt tatctcgcgc cagaacaaca ctttgtagct
     1861 cttgcgttta gcgtgccagc gagtgccgtg aacaacctgg cgtaaagcag aggagatatt
     1921 caaagccgac cttaattgca gaaagtgaaa ccacatttca aataatgagg gagaatcagc
     1981 aaagctgcaa agattttcgc caacaaattg tctgcaaatg caacaaactg ttgatagaaa
     2041 cggcaaacag ttggggaatt taaaaatcgg gtttgacaaa agatttttcg ccgttaagat
     2101 gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta
     2161 tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc ttttatagcg
     2221 ggctttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca cctgccgctt
     2281 gatatcctca actgacagtc ctgcattaca aagttccaga aagcgccaga catagttacg
     2341 ctgaagttgt cctcgcttca gtcccaacca gacagtatta gcatcgaaaa gatgccgcgt
     2401 atccagacgg attaaattct cttcctcttg ttcgccgctg gattgttcgg caactaatcc
     2461 gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgcg cacttaatac
     2521 aatatctgcc agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc gccccgtaat
     2581 cccctgtcgg taagtgatta acggccactt cgctattgat tccagcgtca atggtgtaat
     2641 ttgcgttaag ggatgatcga gtggaacaag caaactatgg tgccaacgaa accacgggaa
     2701 ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat cagcttcgcc
     2761 attttgcaac aacgtcgcaa tttcctgtgg cgtcccctgg attagctcga gccgaacctc
     2821 cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag ctataacgtg cctgagtatg
     2881 cgtcgttgca atagtgagaa cgccagacgt atcgttggta aacaggtctg caagccgacg
     2941 aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt tgcccggttc
     3001 agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc
     3061 cagttcccga atatgacggc tgacgccgga ctgtgaggta aaaagcatat tcgcaacctc
     3121 tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt ggaaattcac
     3181 ggtaaactcc gggcagttca gatttcccgt tattgttaaa gtctaatgcc cggcataaca
     3241 aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac caatt
//
LOCUS       DAZOZD010000350         3292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOZD010000350 DAZOZD010000000
VERSION     DAZOZD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.355"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            95..523
                     /gene="uspC"
                     /locus_tag="ACHUPB_004274"
     CDS             95..523
                     /gene="uspC"
                     /locus_tag="ACHUPB_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR0760914.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(530..1954)
                     /gene="otsA"
                     /locus_tag="ACHUPB_004275"
     CDS             complement(530..1954)
                     /gene="otsA"
                     /locus_tag="ACHUPB_004275"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR0760915.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1929..2729)
                     /gene="otsB"
                     /locus_tag="ACHUPB_004276"
     CDS             complement(1929..2729)
                     /gene="otsB"
                     /locus_tag="ACHUPB_004276"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0760916.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2896..>3292)
                     /gene="araH"
                     /locus_tag="ACHUPB_004277"
     CDS             complement(2896..>3292)
                     /gene="araH"
                     /locus_tag="ACHUPB_004277"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0760917.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT          827 a          829 c          783 g          853 t
ORIGIN      
        1 tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
       61 tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc
      121 tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc
      181 agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt
      241 agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct
      301 tgataagttc attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga
      361 gttaagcgaa catattctgg aagtatgtca caaacatcat ttcgatttgg tgatttgcgg
      421 taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc
      481 aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct
      541 ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct
      601 ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca
      661 gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg
      721 cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg
      781 ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa
      841 catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag
      901 agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg
      961 gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat
     1021 gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac
     1081 gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct
     1141 ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca
     1201 gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt
     1261 ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat
     1321 ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca
     1381 gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg
     1441 tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga
     1501 aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat
     1561 aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca
     1621 acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc
     1681 gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt
     1741 cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg
     1801 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
     1861 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
     1921 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
     1981 ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc
     2041 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
     2101 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
     2161 ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc
     2221 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
     2281 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
     2341 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc
     2401 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
     2461 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
     2521 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
     2581 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
     2641 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
     2701 tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtt
     2761 ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt
     2821 gcaatgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg
     2881 ttgtggaaaa aagcatcaga caatgcgttt cgctttttgc ttgtaacggt cgaagatcac
     2941 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
     3001 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
     3061 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc
     3121 ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc
     3181 tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtac
     3241 acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac ga
//
LOCUS       DAZOZD010000351         3280 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOZD010000351 DAZOZD010000000
VERSION     DAZOZD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.356"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            335..1054
                     /locus_tag="ACHUPB_004278"
     CDS             335..1054
                     /locus_tag="ACHUPB_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0760918.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1065..2492
                     /locus_tag="ACHUPB_004279"
     CDS             1065..2492
                     /locus_tag="ACHUPB_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0760919.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2485..3180
                     /locus_tag="ACHUPB_004280"
     CDS             2485..3180
                     /locus_tag="ACHUPB_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0760920.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          858 a          688 c          924 g          810 t
ORIGIN      
        1 tattgtcggt gatttttttg tacaccatca gtgtatatct caataacccc tgaataagta
       61 gctctgaata ggtataaggg atgtagcctt tttaaatgga tttcttatgc caaaaatgaa
      121 cgtcgatttc cccataaaat gtgagcgatg ccgaaagaaa taaaattagt tattgcattt
      181 gaagtttgga tgaaagatta tcatttgtca cacaaatgag gggtggtatg ttgctagcca
      241 attaaaaaag aacgccatat ttattgatga ttgatccccc ggaaggaggg gaaccatcgg
      301 gcataaatgg gcatgaagta atggagtatt agttgtgaat gtcaatttct ttgtcacctg
      361 tattggtgac gccctgaaat caagaatggc acgagactcc gtgctgctac tggaaaaact
      421 cggctgtcgc gtaaatttcc cggagaaaca gggatgctgc ggtcagcctg cgatcaatag
      481 cggttatatc aaagaagcga ttccagggat gaaaaatctg atcgccgcac tggaggataa
      541 cgacgatccc attatttcac cggctggctc ttgcacctat gccgtaaaaa gttacccgac
      601 gtatctggcg gatgaacctg aatgggcatc acgtgccgaa aaggttgccg cgcgtatgca
      661 ggatctcacc tcttttattg ttaataaatt aggggtagtc gatgtaggtg ccagtttgca
      721 agggagagct gtgtatcacc catcttgtag cctggcccgt aagctgggag tgaaggacga
      781 gccacttacg ctgctgaaaa atgtgcgtgg actggagctg ttgacctttg ctgaacagga
      841 tacctgctgc ggatttggcg gcacgttctc ggtcaaaatg gccgaaatat ccggcgagat
      901 ggtgaaagaa aaggttgtgc acctgatgga agtccgccct gagtatttaa ttggtgctga
      961 cgtgagttgc ctgctgaaca tcagtgggcg attacaacgg gaagggcaga aagtcaaagt
     1021 gatgcatatt gctgaagtgt tgatgagccg ctgaggatat aaagatgtcg atcaaaacca
     1081 gtaatacaga ttttaagaca cgcatccgtc agcaaattga agatccgatc atgcgcaaag
     1141 cggtggcaaa cgcgcagcag cgtattgggg caaatcggca aaaaatggtc gatgaattgg
     1201 ggcactggga ggagtggcgc gatcgggccg cccagatacg tgatcatgtt ctgagtaatc
     1261 tcgacgctta tctgtaccag ctctcagaaa aagtgacgca aaacggcggt cacgtctggt
     1321 ttgcaaaaac caaagaagac gctacccgct acattttaca ggttgcccaa cgcaaaaatg
     1381 cccggaaggt ggtgaaatct aaatcgatgg tgaccgaaga gattggtgtc aatcatgtgt
     1441 tgcaggatgc tggcattcag gtgattgaaa ccgatctggg tgaatatatt ctccagctgg
     1501 atcaagatcc gccatctcat gttgtggtcc cggcaattca taaagatcgc catcagatcc
     1561 gtcgagtgct acacgaacgt ctgggctatg aggggccgga aacgcctgaa gcgatgacct
     1621 tattcatccg gcaaaaaatc cgcgaagatt tcctcagtgc tgaaataggt attaccggct
     1681 gtaatttcgc ggtggcagag acaggttcgg tatgcctggt gaccaatgaa ggtaatgcgc
     1741 gaatgtgtac cacgctgcct aaaacgcata ttgctgtgat gggaatggag cgtattgccc
     1801 ccacgtttgc cgaggtagat gtattgatca ccatgctggc gcgcagtgcc gttggtgcac
     1861 gtttgacggg atacaacacc tggctgacag gaccgcgcga agcggggcac gttgatggtc
     1921 ctgaagagtt tcatctggtt attgtcgata acgggcgttc tgaggtgctg gcctctgaat
     1981 ttcgggatgt gctgcgctgt attcgctgcg gggcttgtat gaatacttgt ccggcatatc
     2041 gccatattgg cggtcatgga tatggctcta tttatccagg gccaattggt gcggtgattt
     2101 ctccgctact tggcggctat aaagatttta aagatttacc ctacgcctgc tctttatgca
     2161 ctgcttgtga cagcgtgtgt ccggtgcgta ttccgctgtc aaaactgatt ttgcgtcatc
     2221 gtcgggtgat ggctgaaaaa gggatcaccg caaaagcaga gcaacgggcg ataaaaatgt
     2281 tcgcttatgc caatagtcat ccaggattgt ggaaagtcgg gatgatggcc ggcgctcatg
     2341 cggcaagctg gtttatcaat ggcggcaaaa caccactcaa atttggcgcg attagcgact
     2401 ggatggaagc acgcgatctt cctgaagctg acggagagag tttccgtagt tggtttaaga
     2461 aacatcaggc gcaggagaaa aagaatggat aatcggagcg aatttttgaa taacgttgct
     2521 caggcactgg gtcgcccgct gcgacttgaa ccgcaagcag aagatgcgcc gcttaacaac
     2581 tatgctaacg agcggcttac ccaacttaac caacagcagc gctgtgacgc gtttattcag
     2641 tttgccagcg atgttatgtt gacgcgctgt gagctgacca gcgaggcgaa ggcggcagaa
     2701 gctgcaatac gtctgtgtaa agagctggga gatcagtcgg tcgtgattag cggtgacacg
     2761 aggctggagg aattggggat tagtgaacgt ttgcagcagg aatgcaatgc cgttgtttgg
     2821 gatccggcga aaggtgccga gaatatctcg caggcagagc aggctaaagt gggtgtcgtg
     2881 tatgctgaat atggtttaac cgaatcggga ggcgtggttc ttttttccgc cgtcgagcgc
     2941 gggcgttcat tgagcctgct cccggaatct tctcttttta tcctgcgtaa aagcacaatc
     3001 ctgccgcgtg tagcgcaact cgcagaaaaa ttgcatcaga aagcgcaggc cggtgaacga
     3061 atgccgtcat gtattaacat catcagtggt cccagctcaa cggcggatat tgagcttatc
     3121 aaagtcgtcg gggttcatgg tccggtgaaa gcggtgtatc tgattattga ggattgctga
     3181 gagagatatg agatctgaac ataaacctga agaaggcata cgaggttgaa aatagcgtta
     3241 aggacaacct gacttaaccc cgttccgtca tggttatgct
//
LOCUS       DAZOZD010000352         3270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOZD010000352 DAZOZD010000000
VERSION     DAZOZD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.357"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..951
                     /locus_tag="ACHUPB_004281"
                     /pseudo
     CDS             1..951
                     /locus_tag="ACHUPB_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(989..1888)
                     /gene="nhaR"
                     /locus_tag="ACHUPB_004282"
     CDS             complement(989..1888)
                     /gene="nhaR"
                     /locus_tag="ACHUPB_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0760921.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(1954..3120)
                     /gene="nhaA"
                     /locus_tag="ACHUPB_004283"
     CDS             complement(1954..3120)
                     /gene="nhaA"
                     /locus_tag="ACHUPB_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0760922.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT          966 a          792 c          698 g          814 t
ORIGIN      
        1 ttttattaat caccttgagt ctatattctt ttattgtgca aagtgctccc tgtgcattga
       61 ccaatgtcgg ggaacaacgt ggaacatata tactcaagcc actgagtatg aaaggtaatc
      121 tcaccgccga aaaaattgaa aacaccggaa atatcattga gtgtgcagac ttacaagaga
      181 cgaccaacgc cattaaagtt cattttttaa cacagaacgc tttaaggttt aaagtcggaa
      241 gtcgtcatta tattattaat ttcccctttg aatctgacgg taaaaaaaat agagcttcca
      301 ggaaggtcat cttataattt agaagaaatg ctatctgcga taaatttcac cattccatac
      361 tctatcgcat ctgtatcaga tagttccaac accgcagata tttttccagg aactcctttt
      421 cctttgttgt tcgaaattga agtgagttca tgtacaggga ataatactaa ttgtaatagc
      481 gtccgcttta attataattt aaatactatt ttacaggtaa atttaatgac ctgtggtttt
      541 gaagatcaat ccatcgacac tggacgattt gtactgtctc gtatcaataa tgaaaattat
      601 cagtttcatc atataagatt tgactgcatc cagggcgagc aaggtgaatt agtttttgaa
      661 cccagtgatg ttgaatatta ctttgaacca gtgacgatac aggagtcagg aaccagcata
      721 ttaaaaaatg agttagaaaa cagtgaggac ggtgcgggtc aagtaggctt tcaaccctcc
      781 aatgatggta cgaatgaaat ccagtatggt aaaagcaatt actactcttt tcagcatccc
      841 catgaaggaa gtaatcagat tcctctattt attcgcccgc gtacatatgg aaataatgtc
      901 tcaagcgggc aaataatgag ccgagtcaaa atcgtggtta tgtacaacta atctatctcc
      961 cccaccttaa ctttggggga ggtgccgatt aacgcgccgc tggactaaaa agcgcagaat
     1021 aatccgtatt gcagattcgc tgtaccgccg ggtgctgaat catccgctca gcaaaaatag
     1081 catggtactc ttccatcaca ttctcgacgc gaccaatttc tacgacagtt ttatcggcat
     1141 aaaagtcata tgcataaagc gttggggcaa cgaagattgc attgtgcatc gcaccaaaag
     1201 ctttcatcaa cgcggcatca tcaaactcgc cgaggatttc tacgtttaat ccctgggagt
     1261 taaaccagtt aagcaatttg cgccctaaca ttgaacgtcg cccaggaatc aaaagtcgcc
     1321 gttcttccag acaagccggg aacgattttt ctggtggtgg atttgtacac cagaaactca
     1381 cgccacattc gccaattctc acggagaaca ggccttcctg ctgcgtagag tctatcggac
     1441 agtcagaaat gatcatatcc agtttatgct gacttaattg ctccagcagc atttcgtggg
     1501 tggattcgaa gcagcgaaga tgaatgggct cgccttctac cactgcggcg ttaagtacgc
     1561 tactgaccag gcgtttggaa agtgcatcag ccacgccaac gtcaaacaat aaattggatt
     1621 ctttgcgata gttcacaata tccagcattt cctggcttaa ggtgaacatt ttatcggcat
     1681 agcgatagac cagttctccc agctcgctgg gttcgagacc acgtcccttg cgtttaaata
     1741 atttgccttg caggcgctct tccagcgctc gaatctgtcc ggtaatggtt tgtggagtta
     1801 aataaagcgc ctccgctgcg ccaaccacgg aaccttcttt atagacatgc cagaagtaat
     1861 acaagtggtt gtaattgata tgagacatgc tcatttctct ccctgataac aatgaaaagg
     1921 gagccgttta tggctccccg gtaaaccgtc ctgtcaaact gatggacgca aacgaacgcg
     1981 taaccagctg tatccaatta ccgccgaaga gatagaaccg accaggatac cgagtttcgc
     2041 ccagttaatc agttctggat ctacgctacc aaaggccagg ctggcaataa agatagacat
     2101 agtaaaaccg ataccgcaca ggatccccac cgccataatt tgctgataag tcgttccctc
     2161 aggcagatgc gccagtttca aacgcagcgc caaccagcag aacagactaa tccccagcgg
     2221 tttgccaatc agcaagccag cgatgatccc caatggcaga atggaggtca agccatccag
     2281 cgtgacgcct tgcagtgaaa cgccagcatt agcaaatgca aacagcggta aaatcagata
     2341 cgccacccac gggtgcaata catgctccag tcgcttcgct ggagaacgcc catgcttctc
     2401 tttcaaagga ataaagaagc cgacaattac ccccgccagg gttgcgtgaa cccccgattt
     2461 caacaccgca gtccacaaca ccacgccaac aagaatatag acgcccgtgc ggcgtacacc
     2521 acacagattc aataccgcga gtaccgcaat tgctacagcc gcgacgccaa gagaggccat
     2581 cgataagtca ttagtgtaga acaatgcgat gataatgatg gccccaagat cgtcgataat
     2641 agccagagcc atcaaaaaga tcttcagtgc taacggaaca cgacttccca acagcgccag
     2701 cacaccaagt gcaaaggcaa tgtcagtagc cgccgggatc gcccaccctt cgcgggtaat
     2761 cggatcggca tagttaaagg ccagatagag taatgccgga acaatcatcc caccaatagc
     2821 ggcgataacc ggaaatgcgg cctggcgtaa gctggctagc gatccttgca tcagttcacg
     2881 tttaacttcc agaccgacta acaggaaaaa taccgccatc agcgcgtcat ttatccataa
     2941 cagcatgttt ttgttgattt cgagtgaacc aacccggagc tgaaccggcg tctccagaaa
     3001 gtcgtgatac catccactgg ttgcgccgct gttggccatc atcatcgcca ggatagcggc
     3061 aatgataaga ataatgcctc ccgaggcatc actgctaaag aatcgatgca gatgtttcac
     3121 tttttatttc tctttcaggt gaatagatcg tttttactat tttaccccgc acaaatcatc
     3181 gttaaaatca gattatagtg taggaataac tcgtttttta cgagcaatta aaggatttgc
     3241 gccatcagcg gtgagatgag tttttgacgg
//
LOCUS       DAZOZD010000353         3267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZOZD010000353 DAZOZD010000000
VERSION     DAZOZD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.358"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1337
                     /gene="ipaB"
                     /locus_tag="ACHUPB_004284"
     CDS             <1..1337
                     /gene="ipaB"
                     /locus_tag="ACHUPB_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR0760923.1"
                     /translation="YEKQINKLKNADSKIKDLENKINQIQTRLSELDPDSPEKKKLSR
                     EEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQLST
                     QQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYAAE
                     VRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQAAT
                     GNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGALVL
                     VAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARLNK
                     FLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGSVASAVFQNSASTNLADLTLS
                     KYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQANRTDVAKAILQQTTA"
     gene            1300..2448
                     /gene="ipaC"
                     /locus_tag="ACHUPB_004285"
     CDS             1300..2448
                     /gene="ipaC"
                     /locus_tag="ACHUPB_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR0760924.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKSTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            2499..>3267
                     /gene="ipaD"
                     /locus_tag="ACHUPB_004286"
     CDS             2499..>3267
                     /gene="ipaD"
                     /locus_tag="ACHUPB_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR0760925.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKKKYEDKPLYPATNTV
                     SQKEADKWLTELGGTIGKVSKKNGGYVVNINMTPIDNM"
BASE COUNT         1194 a          675 c          573 g          825 t
ORIGIN      
        1 actatgaaaa acaaattaat aaactaaaaa acgcagattc taaaataaaa gacctagaaa
       61 ataaaattaa ccaaattcaa acaagattat ccgaactcga cccagattca ccagaaaaga
      121 aaaaattaag ccgggaagaa atacaactca ctatcaaaaa agacgcagca gttaaagaca
      181 ggacattgat tgagcagaaa accctgtcaa ttcatagcaa acttacagat aaatcaatgc
      241 aactcgaaaa agaaatagac tctttttctg cattttcaaa cacagcatct gctgaacagc
      301 tatcaaccca gcagaaatca ttaaccggac ttgccagtgt tactcaattg atggcaacct
      361 ttattcaact agttggaaaa aataatgaag aatctttaaa aaatgatctg gctctattcc
      421 agtctctcca agaatcaaga aaaactgaaa tggagagaaa atctgatgag tatgctgctg
      481 aagtacgtaa agcagaagaa ctcaacagag taatgggttg tgttgggaaa atacttgggg
      541 cacttttaac tatcgttagt gttgttgcag cagctttttc tggaggagcc tctctagcac
      601 tggcagctgt tggtttagct cttatggtta cggatgctat agtacaagca gcgaccggca
      661 attccttcat ggaacaagcc ctgaatccga tcatgaaagc agtcattgaa cccttaatca
      721 aactcctttc agatgcattt acaaaaatgc tcgaaggctt gggcgtcgac tcgaaaaaag
      781 ccaaaatgat tggctctatt ctgggggcaa tcgcaggcgc tcttgtcctg gttgcagcag
      841 tagttctcgt agccactgtt ggtaaacagg cagcagcaaa acttgcagag aatattggca
      901 aaataatagg taaaaccctc acagacctta taccaaagtt tctcaagaat ttttcttctc
      961 aactggacga tttaatcact aatgctgttg ccagattaaa taaatttctt ggtgcagcgg
     1021 gtgatgaagt aatatccaaa caaattattt ccacccattt aaaccaagca gttttattag
     1081 gagaaagtgt taactctgcc acacaagcgg gaggaagtgt cgcttctgct gttttccaga
     1141 acagcgcgtc tacaaatcta gcagacctga cattatcgaa atatcaagtt gaacaactgt
     1201 caaaatatat cagtgaagca atagaaaaat tcggccaatt gcaggaagta attgcagatc
     1261 tattagcctc aatgtccaac tctcaggcta atagaactga tgttgcaaaa gcaattttgc
     1321 aacaaactac tgcttgatac aaataaggag aatgttatgg aaattcaaaa cacaaaatca
     1381 acccagattt tatatacaga tatatccaca aaacaaactc aaagttcttc cgaaacacaa
     1441 aaatcacaaa attatcagca gattgcagcg catattccac ttaatgtcgg taaaaatccc
     1501 gtattaacaa ccacattaaa tgatgatcaa cttttaaagt tatcagagca ggttcagcat
     1561 gattcagaaa tcattgctcg ccttactgac aaaaagatga aagatctttc agagatgagt
     1621 cacaccctta ctccagagaa cactctggat atttccagtc tttcttctaa tgctgtttct
     1681 ttaattatta gtgtagccgt tctactttct gctctccgca ctgcagaaac taaattgggc
     1741 tctcaattgt cattgattgc gttcgatgct acaaaatcag ctgcagagaa cattgttcgg
     1801 caaggcctgg cagccctatc atcaagcatt actggagcag tcacacaagt aggtataacg
     1861 ggtatcggtg ccaaaaaaac gcattcaggg attagcgacc aaaaaggagc cttaagaaag
     1921 aaccttgcca ctgctcaatc tcttgaaaaa gagcttgcag gttctaaatt agggttaaat
     1981 aaacaaatag atacaaatat cacctcacca caaactaact ctagcacaaa atttttaggt
     2041 aaaaataaac tggcgccaga taatatatcc ctgtcaactg aacataaaac ttctcttagt
     2101 tctcccgata tttctttgca ggataaaatt gacacccaga gaagaactta cgagctcaat
     2161 accctttctg cgcagcaaaa acaaaatatt ggccgtgcaa caatggacac atcagccgtt
     2221 gctggtaata tatccacatc aggagggcgt tatgcatctg ctcttgaaga agaagaacaa
     2281 ctaatcagtc aggccagcag taaacaagca gaggaagcat cccaagtatc taaagaagca
     2341 tcccaagcga caaatcaatt aatacaaaaa ttattgaata taattgacag catcacccaa
     2401 tcaaggaatt cgacagccag tcagattgct ggtaacattc gagcttaata tatccaagag
     2461 ccataataat atatggctct tcctgtaagg aaataaccat gaatataaca actctgacta
     2521 atagtatttc cacctcatca ttcagtccaa acaataccaa cggttcatca accgaaacag
     2581 ttaattctga tataaaaaca acgaccagtt ctcatcctgt aagttccctt actatgctca
     2641 acgacaccct tcataatatc agaacaacaa atcaggcatt aaagaaagag ctttcacaaa
     2701 aaacgttgac taaaacatcg ctagaagaaa tagcattaca ttcatctcag attagcatgg
     2761 atgtaaataa atccgctcaa ctattggata ttctttccag gaacgaatat ccaattaata
     2821 aagacgcaag agaattatta cattcagccc cgaaagaagc cgagcttgat ggagatcaaa
     2881 tgatatctca tagagaactg tgggctaaaa ttgcaaactc catcaatgat attaatgaac
     2941 agtatctgaa agtatatgaa catgccgtta gttcatatac tcaaatgtat caagatttta
     3001 gcgctgttct ttccagtctt gccggctgga tctctcccgg aggtaacgac ggaaactccg
     3061 tgaaattaca agtcaactcg cttaaaaaag cattggaaga actcaagaaa aaatatgaag
     3121 ataaaccgct atatccagca actaatactg ttagtcagaa agaagcagat aaatggctta
     3181 cagaattagg tggaacaatc ggcaaggtat ctaaaaaaaa cgggggatat gttgtcaata
     3241 taaacatgac cccaatagac aatatgt
//
LOCUS       DAZOZD010000354         3265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOZD010000354 DAZOZD010000000
VERSION     DAZOZD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.359"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            216..1898
                     /locus_tag="ACHUPB_004287"
     CDS             216..1898
                     /locus_tag="ACHUPB_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0760926.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1950..3107
                     /locus_tag="ACHUPB_004288"
     CDS             1950..3107
                     /locus_tag="ACHUPB_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0760927.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3073..3195)
                     /locus_tag="ACHUPB_004289"
     CDS             complement(3073..3195)
                     /locus_tag="ACHUPB_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760928.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          999 a          692 c          645 g          929 t
ORIGIN      
        1 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
       61 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
      121 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
      181 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
      241 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
      301 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gattttacgc
      361 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
      421 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
      481 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
      541 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
      601 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
      661 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
      721 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
      781 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
      841 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
      901 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
      961 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     1021 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     1081 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     1141 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     1201 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     1261 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     1321 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     1381 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     1441 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     1501 aacaaggcgc gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     1561 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     1621 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     1681 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     1741 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     1801 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     1861 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     1921 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     1981 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     2041 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gaagcgtctg
     2101 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     2161 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     2221 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgaat
     2281 ttctggttgg tatctcgatc gatggccccc tggagttaca tgaccgttac agacgcagta
     2341 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     2401 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     2461 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctagaaa
     2521 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     2581 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     2641 aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag ctttgtcagc cgttttttgg
     2701 ggaatgggca tacaagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     2761 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     2821 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     2881 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     2941 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     3001 ttttttcaac catggtacct tatatgaacg ccatggttga gttagctaag aacagagtac
     3061 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt
     3121 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     3181 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca
     3241 gggcattagc aatacccttg attat
//
LOCUS       DAZOZD010000355         3249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOZD010000355 DAZOZD010000000
VERSION     DAZOZD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.360"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..1898
                     /gene="tssM"
                     /locus_tag="ACHUPB_004290"
                     /pseudo
     CDS             1..1898
                     /gene="tssM"
                     /locus_tag="ACHUPB_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..>3249
                     /locus_tag="ACHUPB_004291"
     CDS             1909..>3249
                     /locus_tag="ACHUPB_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFR0760929.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTT"
BASE COUNT          817 a          888 c          920 g          624 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacg
//
LOCUS       DAZOZD010000356         3246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOZD010000356 DAZOZD010000000
VERSION     DAZOZD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.361"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..401)
                     /locus_tag="ACHUPB_004292"
     CDS             complement(<1..401)
                     /locus_tag="ACHUPB_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0760930.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
     gene            453..1058
                     /gene="wrbA"
                     /locus_tag="ACHUPB_004293"
     CDS             453..1058
                     /gene="wrbA"
                     /locus_tag="ACHUPB_004293"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0760931.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1079..1306
                     /locus_tag="ACHUPB_004294"
     CDS             1079..1306
                     /locus_tag="ACHUPB_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0760932.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(1344..2585)
                     /gene="agp"
                     /locus_tag="ACHUPB_004295"
     CDS             complement(1344..2585)
                     /gene="agp"
                     /locus_tag="ACHUPB_004295"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0760933.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2879..>3246)
                     /locus_tag="ACHUPB_004296"
     CDS             complement(2879..>3246)
                     /locus_tag="ACHUPB_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760934.1"
                     /translation="FIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKVNEVKNFL
                     TALSDSICSLFIVYSNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAY
                     ELNNKERNAQIDFLTRYAI"
BASE COUNT          837 a          785 c          762 g          862 t
ORIGIN      
        1 aacttcacag ggtctgttaa ggctgacttg tcgtagagtt caccatcacc cagagtcagt
       61 atagagaccc ccttcctcag tatctgcctg acatcatcta tagctgtgtc gatatcagcc
      121 ctactgaagc ggtcaaagtt ctctacaaca atcaaggaac cttctggaat agccccagac
      181 ataacatttt caaacatacg tctgaagtca gaaccttgat tcttatgttg ctggtgaaaa
      241 ccagatttac ccttatcaac atatctgtga gcaggaagaa ggtaataatc gtttttgtgc
      301 ttagagtaga aagcatcaat gaggtttgtt tgccgtctta aggagtctcc tgagtcttgc
      361 tctttagtac tccacctgat gtatgtcact agcagcttca tatcttcgga ctatgcttat
      421 ctaagtggtt cattgaacag gagaacaatg atatgagtgt taaggcaaga atactagtgc
      481 tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa
      541 gcaaagtcga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat
      601 tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg
      661 ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa
      721 tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac
      781 tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa
      841 cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac gcagcgcagg
      901 aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag
      961 gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg
     1021 aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat
     1081 gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc
     1141 gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa
     1201 gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc tttgctaagg ccgataaaga
     1261 ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc
     1321 gcttctcgcg ggggagtttt ctgttatttc accgcttcat tcaacacgct atcaaattta
     1381 tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacacgc
     1441 tgagcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc actctgatac
     1501 acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg
     1561 attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc
     1621 agcgccgtta acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt
     1681 gcgctggtgc gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca
     1741 ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac
     1801 aacaccttcc actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa
     1861 ccttcgtaat attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg
     1921 gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa
     1981 cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagc
     2041 tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt
     2101 tcactgaatg cggcggaatc atcggtgatc accgggttaa aggttgggtc catagtgccc
     2161 attttttcct ggtgatgcac aggaatatca caccccggga atgcgccggt aataaagaac
     2221 tgtgcggtcg caacggtacg ttgcagacta ttggcatagg cataaacggt gtccggcggc
     2281 gggcattccc ccgatttcac catcccctgc tctgccagcc attcacgcat gtaatggccc
     2341 atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattct
     2401 ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag cggcgcacgt
     2461 aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt
     2521 gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat tagcgttttg
     2581 ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa atatgacttt
     2641 tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat atacatcttc
     2701 aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg cgtacgccgc
     2761 tgataattgc acctgtggat aaacagaata tcagcggcgt tactaataca gaggcaatag
     2821 taccaataat aagagaagtg aggctaataa caatggtttt tagcacatgc ctttgtcatt
     2881 aaattgcata acgagttaaa aaatcaattt gcgcattcct ttccttattg tttaactcgt
     2941 aagcctgagc aaaaagttca ggagggaggt aaacgtcata atgattgcca gtgtttatta
     3001 aatacccata ctcaaggtcg tcatctgttg aaaaggattc attccctaag gagattgatg
     3061 ccacattgct gtaaacaata aataatgagc aaatgctatc tgaaagagca gtgaggaaat
     3121 ttttcacctc attaaccttc tgctctgggt cggtgtctaa aacaaggcgt gccttgttat
     3181 cattaaatat aaattcgggt aaatgcagta tgcatttttt gtcattcaag caactatcaa
     3241 taaatt
//
LOCUS       DAZOZD010000357         3206 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOZD010000357 DAZOZD010000000
VERSION     DAZOZD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.362"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACHUPB_004297"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACHUPB_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0760935.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACHUPB_004298"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACHUPB_004298"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0760936.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="ACHUPB_004299"
     CDS             1223..1492
                     /locus_tag="ACHUPB_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0760937.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="ACHUPB_004300"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="ACHUPB_004300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0760938.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="ACHUPB_004301"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="ACHUPB_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0760939.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          773 c          778 g          835 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgaca ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtttttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatggccgc
      661 cagccagacg cataaatgcc attacccggc gttctcctgc ttgcagatat tctagtaata
      721 aaggctcaat agcacggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagccg ggaagctaaa acatggggaa
      841 tgtaaggcgt atcgcacgga caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tggcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgttg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcaaggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag agcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gacggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcaca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttggcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgtcc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt caaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAZOZD010000358         3203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOZD010000358 DAZOZD010000000
VERSION     DAZOZD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.363"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            89..805
                     /gene="nanC"
                     /locus_tag="ACHUPB_004302"
     CDS             89..805
                     /gene="nanC"
                     /locus_tag="ACHUPB_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0760940.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            825..1931
                     /gene="nanM"
                     /locus_tag="ACHUPB_004303"
     CDS             825..1931
                     /gene="nanM"
                     /locus_tag="ACHUPB_004303"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0760941.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1996..2976
                     /locus_tag="ACHUPB_004304"
     CDS             1996..2976
                     /locus_tag="ACHUPB_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0760942.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3086..3203
                     /locus_tag="ACHUPB_004305"
                     /pseudo
     CDS             3086..3203
                     /locus_tag="ACHUPB_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          941 a          680 c          756 g          826 t
ORIGIN      
        1 gcctggcaaa aaatattaca caacataaat gctaattgtt tatgcgggct ttgtattgct
       61 ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt
      121 attactgtgc ttttcgtcac cattaatttc tcaggctgcg acactggacg tacgtggtgg
      181 atatcgtagc ggaagccatg cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa
      241 tggatggtgg gcaagcatgg aaagtaatac ctggaatacc attcatgata ataaaaagga
      301 aaatgccgca ctcaatgatg ttcaggttga agttaattac gcgattaaac ttgatgatca
      361 atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac gctacggacc
      421 ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc gctatcgtta
      481 cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg ataacgttca
      541 tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg catggcaaac
      601 gacgctatac agcaaacaga atgattatcg ctatgcaaac cacaagaaat gggcgacgga
      661 aaatgccttt gttctacaat accatatgac gcccgatatt acgccataca tagaatatga
      721 ctaccttgac cgccagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg
      781 cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaactataa
      841 tggcgcttgc tatcatgatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc
      901 ctgtgccatt taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag
      961 gtagcgcagg tacagcatgg tacaagctag atacacaggc caaagataaa aaatggactg
     1021 cgttagctgc attccctggt ggaccgagag atcaagcaac ctctgcattt attgatggca
     1081 atctatatgt gtttggcggc attggcaaaa acagcgaggg attgactcag gtatttaatg
     1141 acgtacacaa atacaacccc aaaaccaata gctgggttaa attgatgtcg ctcgcgccga
     1201 tgggcatggc gggtcatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg
     1261 ttaaccagaa tatcttcaat ggctattttg aagatctcaa cgaggctgga aaagattcaa
     1321 ccgctataga taaaatcaac gcccactatt ttgacaaaaa agcagaagat tatttcttca
     1381 ataagtttct gttgtctttt gatccctcaa cacagcaatg gagttacgct ggcgaatcgc
     1441 cctggtacgg aacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta
     1501 atggcgaagc caaaccagga ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta
     1561 ataatttaaa atggaataag cttgatcccg tctcatcacc agatggcgtc gctggcggtt
     1621 ttgcggggat aagcaatgat tctcttatat ttgccggagg ggccggattc aaaggttcac
     1681 gagaaaatta ccagaacggt aagaactatg cgcatgaagg cctgaaaaaa tcatatagca
     1741 ctgatattca tctttggcat aacgggaaat gggataaatc gggtgaatta tcgcaaggtc
     1801 gggcctacgg agtatcattg ccctggaata atagtttatt aattattggc ggtgaaactg
     1861 caggcggcaa agcggtgacg gattcagttt taatttctgt gaaggataat aaagtcacag
     1921 tacaaaacta acgcttcagg gccccggtaa aggggccctg ctatcaattt gtcatttgta
     1981 ttacgcgaaa attatatgaa cgcaataata tcgcccgatt attactatgt tcttaccgtt
     2041 gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga cagggaagat
     2101 gcgcctcatt ccagaattaa acaattagcg agatttgcgc atacgcatcc cggaggcccg
     2161 ccatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt tcaggatatg
     2221 cagggttatc accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag
     2281 gcactgcata ttgcacggaa attactgccc ttcattccta ataatgcagg gattcttatc
     2341 gttccgtgtt gccgtggcgg atcggctttt accgcgggca gcgaagggac atattcagaa
     2401 cggcacggag ccagccatga tgcttgtcgt tggggaacgg atactccgct gtaccaggat
     2461 ttagtcagca gaacgcgagc cgcactggca aaaaatccgc agaacaaatt cctcggcgta
     2521 tgctggatgc aaggcgaatt tgacttaatg accagtgact acgcgtcaca ccctcaacac
     2581 tttaatcata tggttgaagc ctttcgtagg gatctaaaac aataccattc tcagcttaat
     2641 aatattactg acgcgccatg gttttgcggc gataccacct ggtactggaa agaaaatttc
     2701 ccgcattcgt atgaagctat ttatggcaat tatcaaaata atgttttagc caatattatt
     2761 ttcgtcgact tccagcaaca aggtgaaaga ggactgacga acgcgcctga tgaagacccg
     2821 gacgatttaa gcacgggata ttacggttca gcgtaccggt caccggagaa ctggacgacg
     2881 gcactgcgaa gcagtcattt cagcacggca gcccgtcggg ggattatttc tgacagattt
     2941 gtagaagcaa ttttgcagtt ttggcgcgaa aggtgagcgg ggagtttatt catgggggat
     3001 gtccttgttc gttctcagag tagacaacaa aaaacaaata agatcaaggt aatatctctt
     3061 cacttgcggg ctcgaacagc aggcaattgc caatataata atctggcaca agggtgaaaa
     3121 acgcgcaccg cataaaccgt tgttattgct tacagattag ccggatgcct taatagacat
     3181 ccgcgccttt tcgattatgg ctc
//
LOCUS       DAZOZD010000359         3195 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOZD010000359 DAZOZD010000000
VERSION     DAZOZD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.364"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUPB_004306"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUPB_004306"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0760943.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUPB_004307"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUPB_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0760944.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUPB_004308"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUPB_004308"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0760945.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUPB_004309"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUPB_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0760946.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          747 a          858 c          767 g          823 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaa
//
LOCUS       DAZOZD010000360         3177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOZD010000360 DAZOZD010000000
VERSION     DAZOZD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.365"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUPB_004310"
     CDS             <1..493
                     /locus_tag="ACHUPB_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0760947.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUPB_004311"
     CDS             493..1083
                     /locus_tag="ACHUPB_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0760948.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHUPB_004312"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHUPB_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHUPB_004313"
     CDS             2170..2658
                     /locus_tag="ACHUPB_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0760949.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>3177
                     /locus_tag="ACHUPB_004314"
     CDS             2682..>3177
                     /locus_tag="ACHUPB_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0760950.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEI"
BASE COUNT          822 a          709 c          914 g          732 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacgacga ctcaaagccc ggcacgctga
     2941 atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc aaacgctttg
     3001 aggaaatggt ggcgcaggcg cagcagagcg ccggggccgc agccggaaac gcacagcaga
     3061 cggcgcagga tgtggcggca gccgcaggtt atgcccgcgc agcagagcag gccaaaaatg
     3121 acattgatgc tgcgctgacc ggcactctga aaacggctaa ccatctgtct gaaatcg
//
LOCUS       DAZOZD010000361         3174 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOZD010000361 DAZOZD010000000
VERSION     DAZOZD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.366"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            532..2385
                     /locus_tag="ACHUPB_004315"
     CDS             532..2385
                     /locus_tag="ACHUPB_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0760951.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2536..2751
                     /locus_tag="ACHUPB_004316"
     CDS             2536..2751
                     /locus_tag="ACHUPB_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0760952.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2756..3070
                     /locus_tag="ACHUPB_004317"
     CDS             2756..3070
                     /locus_tag="ACHUPB_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR0760953.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          761 c          885 g          750 t
ORIGIN      
        1 ttccaggccc atccgctccg gtgggccttt ttactgcaga aaacaggttc cccgttaaat
       61 gctatgttgc tcacaattca gtaagttgac agttgcctgt cagactgggc atttgttaaa
      121 aaaatttcgc atggtgaatc cccctgagcg gaggggcgac tggtgacggt ataatctctg
      181 attatcaaaa cgagaatgac gcgggtttag tggcaccggg ctgaactcac cgggaggcac
      241 ccggcaccat gtgcatgatg atacagatac gcggctttag cccctctccg gaggggtttt
      301 cttgtgggca aaaaaagccc gcgctgggag acgcgggcgg caaggaataa acaataaaac
      361 gtgaagtaat atttcagctg gcgaataata ccccatagta atcactctgc gcaactgcgc
      421 ggtctttttc gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta acttccggac
      481 ttcagcccgc tccttatttt actcacaata ttatcccggc cgggaggatt catggcattt
      541 aaacactatg acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg actgacacaa
      601 aaactgaagg agggctggca gccgtttggt agtccggtgg ccataacccc ttataccctg
      661 atgcaggcga ttgcagcaga aggtgatgtg gtcgtcagtg gtgcaactga gccggagtgg
      721 tactacgtca tcgtactggc cgggcaatcc aatgccatgg cttacggtga aggtcttccg
      781 cttccggatt catacgatgc gccccatccg cgcattaagc aactggcccg tcgtaacaca
      841 gtgactcccg gtggtgaagt atgcgtattt aacgacatca ttcctgctga ccattgtctg
      901 catgatgttc aggatatgag tacgattaac catccccggg ctgacctgag caaagggcag
      961 tacggctgtg tcggacaggg cttacatatt gccaaaaaac tgcttccgta tatccctaat
     1021 aatgcgggga tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcacg
     1081 gaggggacat tcagcgagtc cacgggggcc agtcaggatt cggctcgctg gggagtgggt
     1141 aagccgttat atcaggatct gcttttccgc acgaaggcag cattgcagaa aaacccgaaa
     1201 aacgttttgc tggcgatatg ctggatgcag ggggaattcg atatgacgaa tgccagttac
     1261 gcccagcagc cagcagcatt tcttgcaatg gtacagcagt tccgtgctga ccttgccggg
     1321 ctggcggcgc agtgtcacgg tggaagtccg gcatcagtcc cctggatttg tggcgacacg
     1381 acatacgcgt ggaaacaaga acacggtacg caatatgaag tggtatatgg tgcatataaa
     1441 ggtaaagaat cccagcagat ttattttgtt ccctttatga ccgatggtag cggagttaat
     1501 acaccgacaa acaacccgtc agaagatcct gatattgccg ggtctggtta ttacggttcg
     1561 gcatcccgaa cgaacaaaaa ctgggtatca tcaaatcgcc cgacgcattt cagctcatgg
     1621 gcgcgtcgtg gcattattcc cgatcgtatg gcaactgcta ttctgaacgt agccggtcgc
     1681 accttagcct tcattagtgg taaggcaccg gaaatcaaac cctcgcccgg cggcgacacg
     1741 ccatcggggc cgtctgaaga tgcatccata cgcacaatct ccctgttgcc gacagccgga
     1801 gacgctgctg cgcagggctg gagcattaag aatggcggag ttcagttgtc agatggtgta
     1861 tttaagatca ccaagcagag caataaagcc tggtccctga cgcgcccggt ggatgacgca
     1921 gtctccctgc tgacacgggg tggcagactg agctgtaagt ttcgactgtc aggcgcactg
     1981 accaacaacc agttcggtct gggaatttat ctgtataccg atgtagcgtt acctgacgtc
     2041 gtggcgatga ccgggactgg taacccgttc ctgatgtcgt tcttcaccca gaccacagac
     2101 ggcaaactga atctgatgca tcacaagaaa gcaggaaaca caaagttggg cgagttcggg
     2161 aattacagta acgactggct gacgctggag ctggtgttca ccgccggcag tgccacggtt
     2221 actccgaaac tgaatggagt ggctggcccg gcattccagg tcataaaaga cagtctgaca
     2281 ctggggctga atgcgctgac gctgacggat attaccaaaa atgcagcgta tggcgttgag
     2341 atagaaagtc tggtgctgga gataaatgca ccggcatcat cataaaaagt gagccagcca
     2401 aatggaaggt atcgttaaac tcaccggtag tgtcagtggg tcgtctgaga tgcctgcatg
     2461 agttatcaga gccatcagta cttaactggt ggcttttttt attgttgtca gcttccggat
     2521 aacgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     2581 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     2641 tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata tctgactaac
     2701 ctgtatttca aaatcagaga ggaccgtcgt aaggctgcac ggggagagta attcaatgac
     2761 tcaaaactat gaactgattg tgaaagggat ccgcaatttt gagaataaag ttacggtaac
     2821 tttagcgtta cgggacaaaa aacgctttga cggtgaaatt tttgacctgg acatctcgct
     2881 ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca gcagccagaa ggagcatcag
     2941 acaggtcttc ctggatgttg ctgccgggtt atgtgaaggg gacgagctgt tgccagaaac
     3001 gcgcccctgt tcagaggcgc ggtataccat aaaaattaac agttctgata actcgattac
     3061 gggttgttag ctttttgcag ttggctttcc agtatctttc attggtagca tcctgataaa
     3121 tatccatgag cgcaaaaatc aaatacggcc tgtcagctgc tgttctggcg ctga
//
LOCUS       DAZOZD010000362         3151 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOZD010000362 DAZOZD010000000
VERSION     DAZOZD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.367"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..1985
                     /locus_tag="ACHUPB_004318"
                     /pseudo
     CDS             1..1985
                     /locus_tag="ACHUPB_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            2027..2704
                     /gene="ybiX"
                     /locus_tag="ACHUPB_004319"
     CDS             2027..2704
                     /gene="ybiX"
                     /locus_tag="ACHUPB_004319"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0760954.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="ACHUPB_004320"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="ACHUPB_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0760955.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT          787 a          863 c          812 g          689 t
ORIGIN      
        1 gcgtgggtgc gttttttgcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggcg cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgaa
      781 cctggtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccaac tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctgat gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggcaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcaccg
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgtctgtt gctcaccgcc gcgctgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgcgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccaatatc aggcaaccga cgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcat
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtctac aacctgtttg atactgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcgagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga acttgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc ccggtttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtcgacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttgttctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt ccatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgta ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggcgat ctcgtgttgt atccctccag cagccagcat tgcgtgccac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactgg acaacaatat tcagtcgctg aaaagccgct acggcgaaag
     2641 tgaagagatc ctgtcgctgc ttaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgatgcttg aactgcccga gttcgtccct ggattagcta cacttaactg tacaagtatt
     2761 gatatgggga ggtcgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgcggcgaaa
     2881 gcctggatga atgtgaagag tgcggtgccc ccatcccgca ggcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcttata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataattttt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggt ataaaacaag ttcataagta c
//
LOCUS       DAZOZD010000363         3145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOZD010000363 DAZOZD010000000
VERSION     DAZOZD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.368"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            392..1078
                     /locus_tag="ACHUPB_004321"
                     /pseudo
     CDS             392..1078
                     /locus_tag="ACHUPB_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1411..2286
                     /locus_tag="ACHUPB_004322"
     CDS             1411..2286
                     /locus_tag="ACHUPB_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR0760956.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2294..3070
                     /locus_tag="ACHUPB_004323"
     CDS             2294..3070
                     /locus_tag="ACHUPB_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR0760957.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          856 a          735 c          759 g          795 t
ORIGIN      
        1 aaaaactggt cgcagaaaag tcctgttttc agcttggaaa cccggtaatc agaataatgg
       61 ataggggaac aaaaatctct gtatagaacg ctccattgag ggagagaaaa cgtgtcattt
      121 tgacccgttc ggtattcaga acacacaaaa ccgatcgcgg gtcgctttcg tgcatagtta
      181 tgcaacgcgc taaaaacgat tctgacgcat tttttatgtt taccctgttt tttcttgtct
      241 ttttgagttt tttgcgtcag agagcgtctg agagggcacg tacaacggga gttatggtaa
      301 atggatcggg ttttcgggaa ggttcgacag gatttgccgt tgggtgtagt gtaagcgact
      361 gaaaaacaaa cgcccctgaa atcatgtcca gatccggcag aaccagcctc atgcgcagtt
      421 ctggctggtt tttgacgttg atcgcgaggg ggcagcgatt gactggagcg accggaacgc
      481 tcccgcgccg aacatcaccg taaaaaatcc tgtgaacggt catgctcacc tgctctacgc
      541 gctcaatatc gccgtgagaa ccgcgccgga cagctcggtt aaggcgctga aatacgccgc
      601 tgcgattgaa cgttcgctgt gtgagaaact ttgtgctgat gtgaactaca gcggactgat
      661 ttgtaaaaat ccgttccacc tggaatggca ggtgatggag tggcgagagg aagcctatac
      721 cctcgatgaa ctggctgatt atctcgattt gagcgcctca gcgcgccgta gcatcgataa
      781 acattacggg atggggcgaa actgccacct gttcgaaatg acgcgcaaat gggcttacag
      841 ggcgattcgt cagggctggc ccgaattctc acagtggctt gatgccgtga ttcagcgtgt
      901 cgaaatgtac aacgcatcgc ttcccgttcc gctttcaccg cctgaatgtc gggctattgg
      961 caagagcatt gcgaaataca cgcacaggaa cttcacgccg gaaactttcg cacagtatgt
     1021 ggctgatacg cacacattaa cgtacgtttt cgttccattg gccctcaccc cccgataacg
     1081 acccaggtag cgcgccgtat tccccccgcc ggccgttttc ttcgacatgt acatcaggcg
     1141 cggaactcat cggggcgcgg gaatgaaatc ccattcagac tccggataga ttcaagcaag
     1201 cccacttatc gtcaaaatcg gtgttgcaaa aacgggagtg accatagatc cgttttccaa
     1261 gcggacccca taatatcggc tggtaaacat cactcgctag tgcattcttt ttgttggtaa
     1321 tgataatcat ttacattcat tctttgatgt tttatcataa ttaatgtttt gtcaataaat
     1381 atatggaaac atattgtttc agatgggaat atgagaaagt tcatccttat ttctatgata
     1441 acgttagcta gtgttagtct gggagcctgc agacagaatg tcaccataaa gcaggatgcg
     1501 ccagggcaga aagctttcct gactgacggg cagatttacg atttgcacag tggcaaaatt
     1561 atttctagca gcgagctttt ggcggatctt gctaccgctc gacatcttat tattggtgaa
     1621 aaacacgata atgccgagca tcatcagatt gagctttggc ttattcaaaa tttgctcatc
     1681 cagcgaccac aggggagtgt gttgcttgag atgttgacgt cagagcaaca gccccgagtt
     1741 aaccaagtta aatgttggct gaaagataat cctgtggttc gagatagccg tgttcaggaa
     1801 ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg gcgacatcgt catgcagcta
     1861 ttacgtgggc cttacccgct gctcaatgcc aacattggcc gggagcagat gttggcactg
     1921 tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg cacctgtggt acaagaagct
     1981 ctgcgtgaaa ccatcatatc tatgcatgaa ggcaaccttg aaagccagca actgacgtca
     2041 atgctctcta ttcagcagca gcgggatcgc tacatggccc gccagcttct gagtgccccg
     2101 gtaccgtcgt tgttgattgc aggtggttat cacgcgtcaa aaagcatggg cgtaccgtta
     2161 cacatggaag atcttgccac cggcacccat ccagtggtgt tgatgcttgc agaaaaagga
     2221 atgaacatca cagtagatca tgctgactac gtgtggtttg tagcgccaga tacaacaaaa
     2281 aggtaaacca tggatgatga atattctcca cttctcacag tcggtaaaat ggagcagctg
     2341 gtttatctgt tctctactgc ttcatggact gattttcctt gcgtttatct ggcgtttttc
     2401 cgaagttcag cccgctatgt ctcctgcacc ggcgatcatg ctgcaatggg cagaaattat
     2461 agaggctcct tcttctccac tttcgcttcc ggtgggaatc gctcaacagg agtccgcagt
     2521 gacagaagaa aagcaacaaa ctgaggacag acaacaacgt cctgtcactg aagacagtga
     2581 tgcaaccatt gaaataacta ggaagaaaaa gtcatccgat ggagaaaaga aaaagacacg
     2641 tccacctcgt aagataaagg ctcaaaccag cgacagcaac cccacagccg tttccagtaa
     2701 tgctgcacct caggctctgg tcgaatcgtc ccgtattgct gcgcctttta acagtgactc
     2761 aacaaagcga gataattctg aggcatcttg ggaaagcaga gtaaaaggcc acctaaaccg
     2821 ttataaacgt taccctggcg atgccagaaa acgtgcacga accggcacag ccgtcgtgac
     2881 ttttaccgta aacacggaag gcactattgt cagtagtttc ttggaaatct cttcaggaac
     2941 cctttctctt gaccgcgaag ctattgctgt tttagaacgc gctcaaccac ttcccaaacc
     3001 accgcctgaa atattggagg gaggattatt taaagtaaaa atgccgatta cctttaagtt
     3061 gaaggagtaa agggataatt ggccgttcaa gttcatttaa ctgaaattta attttcattt
     3121 ttatgctgaa cattttatca aactt
//
LOCUS       DAZOZD010000364         3134 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOZD010000364 DAZOZD010000000
VERSION     DAZOZD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.369"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHUPB_004324"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHUPB_004324"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0760958.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3122)
                     /locus_tag="ACHUPB_004325"
                     /pseudo
     CDS             complement(1496..3122)
                     /locus_tag="ACHUPB_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT          894 a          722 c          727 g          791 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaa
//
LOCUS       DAZOZD010000365         3133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZOZD010000365 DAZOZD010000000
VERSION     DAZOZD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.370"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            95..577
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004326"
                     /pseudo
     CDS             95..577
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(800..1156)
                     /locus_tag="ACHUPB_004327"
     CDS             complement(800..1156)
                     /locus_tag="ACHUPB_004327"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR0760959.1"
                     /translation="MIQAQEYIGAGYHWVVDLDLEKFFDRINHDVLMSRIEKRVSDKR
                     VLSLIRRFLNAGVMDAGLVRPVTEGTPQGGVISPLLSNLFLHYAFDMWMQRQCPDVPF
                     ERYADDGVPRRRAPGT"
     gene            complement(1217..1420)
                     /locus_tag="ACHUPB_004328"
     CDS             complement(1217..1420)
                     /locus_tag="ACHUPB_004328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760960.1"
                     /translation="MRVSELPDYLRHHWPELKAQLLSGRYRPSPVRRVSILKPGGGER
                     LLGIQMVVDRFIQQAMMQVLQAL"
     gene            1446..2228
                     /locus_tag="ACHUPB_004329"
                     /pseudo
     CDS             1446..2228
                     /locus_tag="ACHUPB_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2806..>3133
                     /locus_tag="ACHUPB_004330"
     CDS             2806..>3133
                     /locus_tag="ACHUPB_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0760961.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGD"
BASE COUNT          666 a          890 c          837 g          740 t
ORIGIN      
        1 cggcacggaa ccacggcagc ttgacggctg caatgacgtg tgtcgtctgg cggtggagta
       61 tctgctgtat gcagcccgaa aacgggggct ggaaatcggc atcttctgca ccatccacac
      121 tttacgcctt cactttgaag aacatttacc cctcgttgta gccggacgtc gcctgggcgt
      181 accaaaatca acagtttgta gtatgtttgt gcgcttccgc aaagctggcc tttcatggcc
      241 tctgcccgca ggtatgtcgg agcgggaact tgacgcccgt ctttacggga gtgcctccac
      301 agtacctgtc gtacttactg aaagtaccgt tatgcccgaa gtccccgggg taaaaaagcg
      361 tccccggcgc cccaacttcc cttatgagtt caaaatcgcc ttagtggagc agtcactgca
      421 gcccggagcc tgtgtggcgc agattgcccg tgaaaatgga atcaacgata acctgctctt
      481 caactggcgc catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgccggc
      541 actgcttccc gtgacgttaa cgccggagcc ggataaccat ggttttgaca tcatctatat
      601 gctttcgaca catcatcagc ggttcacttt cgttcgtctc ttcgatccat acctgatcgg
      661 ctcacgtccg accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg
      721 ggtggtttgg tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc
      781 agcatggctg cacagggact cacgtccctg gtgcgcgcct tcgtggcaca ccatcatcgg
      841 cgtacctttc gaagggaacg tcgggacact gacgttgcat ccacatatca aaggcatagt
      901 gcaggaacag atttgagagc aggggcgaga tcacgccgcc ctgcggcgtt ccttctgtca
      961 ccggccttac cagacctgcg tccattaccc ctgcattcag gaacctgcgg ataagtgaca
     1021 acacccgttt atccgatact cgtttctcta tccggctcat cagcacgtcg tgatttatcc
     1081 ggtcgaagaa cttctccaga tcgaggtcga ccacccagtg atacccggcc ccgatatatt
     1141 cctgggcctg tatcactgcc tggtgggccg aacgaccggg acggaacccg tagctgttgt
     1201 cactgaacga gctatctcac agtgcctgca atacctgcat catagcctgc tggatgaagc
     1261 gatccaccac catctggatg cccagcagac gttcgccgcc gccgggcttc agtatggata
     1321 ctcttctcac aggggatggg cggtatctgc cggacagcag ttgcgctttc agctctggcc
     1381 agtggtgcct caggtagtcc ggcagttcac ttacgcgcat gccatcgact cccgccgcac
     1441 cttgattgcg tcttgtcgat ggaacagcaa tcagcgcgcc cgggggcggc agcgctgaat
     1501 ggcgactaca tatgggatat gatcctcata cctgtcagtt cactgatttt gagctaaccg
     1561 acagcagaga cgctgaacgg ctggatcgat ttgcgcaaac ggcagacgag atacgaattg
     1621 ctgaccgggg attcggttcg cgtcccgaat gtatccgctc acttgctttt ggagaagctg
     1681 attatatcgt ccgggtttac tggcgaggat tgcgctggtt aactgcagaa ggaatgcgct
     1741 ttgacatgat ggattttctg cgcgggctgg attgcggtaa gaacggtgaa accactgtaa
     1801 tgataggcaa ttcaggtaat aaaaaagccg gagctccctt tcctgcacgt ctcattgccg
     1861 tatcacttcc tcccgaaaaa gcattaatca gtaaaacccg actgctcagc gagaatcgtc
     1921 gaaaaggacg agtagttcag gcggaaacgc tggaagcagc gggccatgtg ctattgctaa
     1981 catcattatc ggaagatgaa tattcagcag agcaagtggc tgattgttac cgtctgcgat
     2041 ggcaaattga actggctttt aagcggctca aaagtttgct gcacttggat gctttgcgtg
     2101 caaaggaact tgaactcgcg aaagcgtgga tatttgctaa tctactcgcc gcatttttaa
     2161 ttgacgacat aatccagcca tcgctggatt ttccccccag aagtgccgga tccgaaaaga
     2221 agaactaacg cgttgtggag aataacaaaa atggtcatct ggagcttaca ggtggccatt
     2281 cgtgggacag tatccctgac agcctacaaa acgcaattga agaacgcgag gcatcgtctt
     2341 aacgaggcac cgaggcgtcg cgttcttcag atggttcaac ccttaagtta gcgcttatgg
     2401 ggtaaatccc cgcctttttc ctgccggtcc ccggacagag cacaaaggaa gtcgccgccg
     2461 gctccgcttt acccggccat gcggggcatg gccttgtggg ttttcagctc tgtagcctca
     2521 gcgtcgtgtg cgggctgtgc cgtgcctcca tcttagccgg gctggcgttg atgcaagggt
     2581 acgcttcgcc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
     2641 tcacccgctc agatttcgcg gtttcaccct ccaactcccg ccagccgtgt gcgtcaggct
     2701 gcggcttccc ttgcgccctg tgcatccccg ccttatcgcc cggctaagat ggaggcacgg
     2761 caccgcccgg tgtcgcggaa tatgtgaaca acggaggaaa acatcatgca atatgcgaaa
     2821 cctgtcactc tgaatgttga agagtgcgac cgtctctcct ttctgcctta cctgtttggc
     2881 aacgattttc tgtatgccga ggcgtatgtg tacgcgctgg cgcaaaaaat gatgccggaa
     2941 tatcagggcg gcttctggca cttcatccgc ctgccggacg gtggcggtta catgatgccg
     3001 gatggcgacc gtttccacat ggtgaacggt gcaaactggt ttgaccgtac cgtgagtgct
     3061 gatgccgcag gcatcattct gacttctctt gtgattaacc gccagttgtg gctgtaccac
     3121 gacagcgggg atg
//
LOCUS       DAZOZD010000366         3121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZOZD010000366 DAZOZD010000000
VERSION     DAZOZD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.371"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHUPB_004331"
     CDS             complement(<1..1199)
                     /locus_tag="ACHUPB_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR0760962.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHUPB_004332"
     tRNA            1492..1586
                     /locus_tag="ACHUPB_004332"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..3070
                     /locus_tag="ACHUPB_004333"
                     /pseudo
     CDS             1858..3070
                     /locus_tag="ACHUPB_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          905 a          739 c          707 g          770 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa ttctgcatga acaaggcttt aattccgcct ggattgaaat
     2941 gcagttagct catgtggata aaaattccat caggggtacc tataatcatg ccctgtatct
     3001 cgatggccgc cgtgaaatga tgcaatggta cgctgattac attgattcac tctccagccg
     3061 ggaaagttaa gtcagaaata cttttccctg atattttcct caattaagta ttttatcctc
     3121 t
//
LOCUS       DAZOZD010000367         3114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZOZD010000367 DAZOZD010000000
VERSION     DAZOZD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.372"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            389..1291
                     /locus_tag="ACHUPB_004334"
     CDS             389..1291
                     /locus_tag="ACHUPB_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0760963.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEELPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIIDNVAHYGG"
     gene            complement(1242..1472)
                     /locus_tag="ACHUPB_004335"
     CDS             complement(1242..1472)
                     /locus_tag="ACHUPB_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFR0760964.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGARRH
                     PLSEKKRAVARPSPSYFIRHNAPHCRLSTQVR"
     gene            1388..1600
                     /locus_tag="ACHUPB_004336"
     CDS             1388..1600
                     /locus_tag="ACHUPB_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760965.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASPSPFSDERPSSR
                     FRGIALPSKRRRLRYSTVGLTRYRTR"
     gene            1676..2359
                     /locus_tag="ACHUPB_004337"
     CDS             1676..2359
                     /locus_tag="ACHUPB_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0760966.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            2360..2581
                     /locus_tag="ACHUPB_004338"
     CDS             2360..2581
                     /locus_tag="ACHUPB_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760967.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            2511..3029
                     /locus_tag="ACHUPB_004339"
     CDS             2511..3029
                     /locus_tag="ACHUPB_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0760968.1"
                     /translation="MAPIACSWKRIPKFWNWIPASKIEKETRMYGTCETLCRALAAKY
                     PGDTPLMLVIWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRTESGISSGAAM
                     EIINNVRENRQVTVPAELLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQA
                     RALLKNNTREND"
BASE COUNT          672 a          846 c          916 g          680 t
ORIGIN      
        1 gtttatacag tattctaata actgaatccg cacacagtca aatgtcattg ttgtgactaa
       61 aaaacgctca attctctgag tgtgtggctg tatgcctgct gtgggctgct gtgtccagct
      121 tatccacagc attttgtgca cggttctgtg gacaaaatac ctggttaccc aggccgtgcc
      181 ggcacgttaa ccgggctgca tccgatgcaa gtgtgtcgct gtcgccggcc tcctcacccg
      241 gtcacgtttc gtcgttcctc ctccacgcgc tgcggcttcg gggccgcacc tgcattcgta
      301 tgcggtcgcc cggtaacagg tgcggcacgg cctgatggag gccgcatgtg agaggagaat
      361 tcccatgcca aactggtgct cgaatcgtat gtatttttct ggtgaaccgg cacagatcgc
      421 tgagattaaa cgactggcca gcggtgcagt tacaccgctt tatcgccgcg ccacaaatga
      481 aggtattcag ctgtttctgg ccggaagtgc cggacttctg cagatcactg aaaatatccg
      541 ctctgagcag tgccccggag tgacggctgc cggacgtggt gctgtatcac cggaaaatat
      601 cgcgttcacc cgctggctga cacacctgca gaacggtgta ctgctggatg agcaaaactg
      661 cctgatgctg catgaactct ggctgcagag cggtactgga cagcgtcgct gggaagagtt
      721 accggatgat gtcagggaaa ctatcactgt gcatttcacc gcaaaaagag gtgactggtg
      781 tgacatctgg ggtaacgagg atgtatcggt gtggtggaac cgtctgtgtg acaacgtact
      841 gccggaaaaa accatgccgt ttgacctgct gacggttttg ccgacccgcc tggatattga
      901 agtgaatggc tttaacggcg gtgttctgaa cggtgttcct tctgcatatc actggtatac
      961 agaacggtat ggcgtgaagt ggccttgtgg gtatgacctg aatatcagca gtcaggggga
     1021 aaacttcatt caggtagatt tcgacacgcc gtggtgtcag ccggaaagcg acgttgttgc
     1081 agaattaagc cgccgtttcg gctgcacgct ggagcactgg tacgccgaac agggctgcaa
     1141 tttctgtggc tggcagttgt atgagcgcgg agagctcgtt gatgtgctgt ggggggaact
     1201 ggaatggtct tccccgacag atgatgatga gctgccggaa gtcaccggac ctgcgtggat
     1261 aatcgacaat gtggcgcatt atggcggatg aagtatgacg gagacgggcg ggcaaccgcc
     1321 cgtttctttt ccgacaaagg atgtcgccgt gctcctcttt ttactgcgcc ggctgacgcg
     1381 gcgcggcata aacgctgcct gtggtcagtg tcccgcgtcc ggccgggcac gggacgggcg
     1441 cttttaccgc tcccggctgg cgtcggtgac agtgtacgcc agcccgtcgc ccttttctga
     1501 tgaacgccct tcaagccgct ttcgcggcat agccttgccg tcaaaaagac gacggctgcg
     1561 gtattccacg gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggg
     1621 aacgggcacc acaggggtgc cctcccggtg ccctctgtaa gagaaggagt ttggtatgtc
     1681 ccgttttgtc ctcggtaact gcatcgatgt tatggcccgt atccctgata acgccattga
     1741 tttcatcctc accgacccgc cgtacctcgt cggttttcgt gaccgtcagg ggcgtaccat
     1801 tgccggcgat aaaaccgatg aatggctgca accggcctgt aatgagatgt accgcgtact
     1861 caaaaaagac gcgttaatgg tgagcttcta tggctggaat cgcgtcgatc gttttatgtc
     1921 tgcctggaaa aatgcgggat tcagcgttgt tggtcacctg gtcttcacca aaaactacac
     1981 atcgaaggcc gcatatgtgg gctatcgcca cgaatgtgcc tacatcctgg caaaaggccg
     2041 tccacgtctg ccacaaaacc cgctgccgga cgtgctgggc tggaaatatt cgggcaatcg
     2101 ccatcacccg acggaaaagc ccgttaccag cctgcaacca ctgattgaga gcttcacaca
     2161 tccgaacgcg attgtgctgg acccgtttgc aggcagcgga tcaacctgcg tggccgctct
     2221 tcagtccgga cgccggtata tcggtatcga gctgcttgaa cagtatcacc gtgccggaca
     2281 gcaacgactg accgccgtgc aacgggccat gcagcagggg gccgcgaatg ataactggtt
     2341 tgaaccggag gctgcgtaaa tgaactatgc cggacacgaa aaacttcgcg ccgacgtggc
     2401 ggaggtggcc aacaccatgt gtgaccttcg ggccaggctg aacgatatgg agcatcgctg
     2461 tcgctttgat tccgatgtgc ttgttgaacg cctgacacgc cagacgctgt atcgcgccaa
     2521 tcgcctgttc atggaagcgt ataccgaaat tctggaactg gattcctgct tcaaagattg
     2581 agaaggagac gagaatgtac ggaacatgcg aaacgctctg ccgggcgctg gcagcaaagt
     2641 atccgggaga tacgccgctg atgctggtta tctggtcccc ggaagaaatt caggccctcg
     2701 ctgacggaat ggatatttcc ctgtccgatc atgaaatcag gactgtcctg gcgcgcctgg
     2761 aggacatccc ggaagaccag cggactgaat ccggtatttc ttccggtgcg gcgatggaga
     2821 tcatcaataa cgtgagagaa aaccgccagg tgaccgtccc tgctgaactg ctggcgtccc
     2881 tgattcagac cgccgaacag gcactgtgga aacgtgagtg ggccgcccgt gataacggcc
     2941 tcgccgtccc ggaatgcgtc acccgccgac aggcggtggt taatcaggcc cgcgccctgc
     3001 tgaaaaacaa cacacgcgaa aacgactgat gttatcgccg cctgcgggcg gcgattcagg
     3061 gagaccatga atcatgaacg aaacgctgaa tgcactgatt tgtcgccacg cccg
//
LOCUS       DAZOZD010000368         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOZD010000368 DAZOZD010000000
VERSION     DAZOZD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.373"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1258)
                     /locus_tag="ACHUPB_004340"
     CDS             complement(<1..1258)
                     /locus_tag="ACHUPB_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0760969.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="ACHUPB_004341"
     CDS             complement(1415..1624)
                     /locus_tag="ACHUPB_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760970.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="ACHUPB_004342"
     CDS             complement(1647..2357)
                     /locus_tag="ACHUPB_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0760971.1"
                     /translation="MTNELYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="ACHUPB_004343"
     CDS             complement(2873..>3112)
                     /locus_tag="ACHUPB_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760972.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          912 a          654 c          507 g         1039 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataactcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAZOZD010000369         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOZD010000369 DAZOZD010000000
VERSION     DAZOZD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.374"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUPB_004344"
     CDS             <1..173
                     /locus_tag="ACHUPB_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760973.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="ACHUPB_004345"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="ACHUPB_004345"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR0760974.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="ACHUPB_004346"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="ACHUPB_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="ACHUPB_004347"
     CDS             2191..>3046
                     /locus_tag="ACHUPB_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR0760975.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          697 a          710 c          836 g          803 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggagaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAZOZD010000370         3032 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZOZD010000370 DAZOZD010000000
VERSION     DAZOZD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.375"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            150..863
                     /locus_tag="ACHUPB_004348"
     CDS             150..863
                     /locus_tag="ACHUPB_004348"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0760976.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            895..1653
                     /locus_tag="ACHUPB_004349"
     CDS             895..1653
                     /locus_tag="ACHUPB_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760977.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1699..>3032
                     /locus_tag="ACHUPB_004350"
     CDS             1699..>3032
                     /locus_tag="ACHUPB_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0760978.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          684 a          735 c          813 g          800 t
ORIGIN      
        1 ccattaaaaa tatttatttg gttaatatgt ttttatgaaa gcgtaattca ggtcaatgtc
       61 acaattaacc attgtcacaa taaggttgaa cggatattct gagcacggac cacgtaatat
      121 tcaatacatt atttactgcc gggtttttcg tgaatcaacg taatatgtca ataattaatt
      181 ccacgccagt gcgtgttatt gccattgtag gatgtgatgg ttcaggtaaa tcgcccctca
      241 cggcaagcct ggtaaatgaa ctggcagcaa gaatgccaac agaacacatt tatctcgggc
      301 aatcgtccgg gctaattggc gaatggattt cacagctccc tgttattggc gcaccttttg
      361 ggcgttatct gcgaagtaaa gcggcacatg tgcacgaaaa accctcaaca ccgcctggca
      421 atattactgc tctggttatc tatctgcttt cctgctggcg ggcgtacaag tttcgcaaaa
      481 tgttgtgtaa aagccagcaa ggctttctgc tcatcaccga ccgctacccg caggttgaag
      541 tgccgggttt tcgctttgat ggcccgcaat tggcaaaaac cacgggcggt aacggttgga
      601 taaaaatgtt aaggcagcgc gagctgaagc tgtaccaatg gatggcatct tatttgcccg
      661 tattgttgat tcgtcttggc attgatgaac aaaccgcgtt tgcgcgtaaa cctgaccacc
      721 aactggcagc gttacaggaa aaaatcgccg ttacgccgca actgactttc aacggcgcca
      781 ggatccttga actggatggt cggcaaccag ccgatgaaat cctgcaagcg tcactacgtg
      841 caattcacgc cgcgctttcc tgatcaccaa aatggatagg ggatgattta acgaatggat
      901 gcactacaaa cacaaactgt taatagtaca accgcgccgc agcccagcta cattccgggg
      961 ctaattgcgg tggtcgggtg cgatggcacc ggtaaatcca cgctgaccac cgatctggtg
     1021 aaatcattgc aacagcactg gcaaaccgag cggcgctatc tggggctgct ctcgggcgaa
     1081 gacggcgaca aaatcaaacg attaccgttg gttggcgtct ggctggaacg gcgactggcg
     1141 gccaaatcct cgaaaaccca aagcatgaaa accaaatctc cggcgctatg ggcggcggtg
     1201 attatgtact gcttatcgct gcgaagaatg gcgaatctac gcaaggttca gcgactggcg
     1261 caaagtggcg ttctggtggt cagcgatcgc ttcccgcagg ctgaaatttc gggcttttat
     1321 tacgacggac cggggattgg cgtcgaacgt gcgaccggga aaatcagcat gtttctggcg
     1381 cagcgcgaac ggcgtttata ccaacaaatg gcgcaatatc gcccggaatt aattattcgc
     1441 ctgggcattg atattgagac tgccatctcc cgcaagcctg accatgacta tgccgagctg
     1501 caggacaaaa tcggtgtcat gtcgaagatt ggctataacg gcacaaaaat tcttgagatt
     1561 gattccagag cgccctacag cgaagtgctg gaacaggcac agaaggcggt ttctctggtt
     1621 gccatcgttt ctgaccgccg aagtttaact taaggctggc gggatacttt ttactatcaa
     1681 cgataaggtc aggctgaatt ggcgggtttt aacatcaaac attggtttgc agatggcgcg
     1741 tttcgcacca ttattcgcaa tagcgcctgg ttaggctcca gtaatgtcgt gagcgccttg
     1801 ctggggctgt tggcgctctc gtgtgccggt aaagggatga cgcccgccat gtttggcgta
     1861 ctggtgattg tgcaatcgta cgccaagtcg atcagcgatt ttattaagtt tcagacatgg
     1921 cagctggtgg ttcagtacgg aacgccagcc ttgaccaaca ataatccgca gcaatttcgc
     1981 aatgtcgtct cattttcctt ctcgctggat atcgtcagcg gcgcggtggc gattgtcggc
     2041 ggcattgcct tactgccatt cctttcccat tcattaggtc tggatgacca aagcttttgg
     2101 ctggcggcgc tgtattgcac gctcattcct tcaatggctt cctccacgcc gaccggcatt
     2161 ctgcgtgcgg tagatcgctt cgatttaatt gctgtacagc aggcgacgaa accttttctg
     2221 cgcgcagcgg ggagcgtcgt agcctggtat tttgactttg gttttgcggg ttttgttatt
     2281 gcctggtacg tgtcgaatct ggttggcggc accatgtact ggtggtttgc agcgcgcgaa
     2341 ttacgccgca gaaatatcca taatgccttc aaattgaatc tgtttgagtc tgcccgacac
     2401 atcaaaggcg cgtggagttt tgtctggtca accaacattg cccactccat ctggtcggcg
     2461 cgtaactcgt gcagcacagt gctggtgggg atcgtgttag gacccgctgc cgccgggtta
     2521 tttaaaatcg ccatgacatt ctttgacgcc gccggaacgc cagcgggttt gctgggtaaa
     2581 agtttttacc cagaggtgat gcgtttagat ccgcgcacca ccagaccgtg gttgttaggt
     2641 gtgaagtccg gtttactggc gggcggaatc ggtattctgg tggcgctcgc ggtgttgatc
     2701 gccggcaagc cgctcatttc gctggtgttt ggcgttaagt atctcgaagc gtatgacctg
     2761 attcaggtga tgctgggcgc aattgtgatt tcgatgctgg gtttcccgca ggagtcattg
     2821 ctattgatgg cgggaaaaca gcgcgctttt ctcgtggcgc aaaccatcgc ctcaatcggc
     2881 tacatcgtat tgctgttcat gttctgtcat ctgtttggtg tgctgggagc agcgtttgct
     2941 tacttcggtg ggcaatgtct ggatgtggtg ctctcgttaa ttccaacttt aaaggcattt
     3001 tttcagcgtc attccttgct ttataacgca gc
//
LOCUS       DAZOZD010000371         3031 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOZD010000371 DAZOZD010000000
VERSION     DAZOZD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.376"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            68..400
                     /gene="hdeA"
                     /locus_tag="ACHUPB_004351"
     CDS             68..400
                     /gene="hdeA"
                     /locus_tag="ACHUPB_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0760979.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            516..842
                     /gene="hdeB"
                     /locus_tag="ACHUPB_004352"
     CDS             516..842
                     /gene="hdeB"
                     /locus_tag="ACHUPB_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0760980.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            906..1553
                     /locus_tag="ACHUPB_004353"
     CDS             906..1553
                     /locus_tag="ACHUPB_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR0760981.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1595..2125)
                     /locus_tag="ACHUPB_004354"
     CDS             complement(1595..2125)
                     /locus_tag="ACHUPB_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0760982.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2281..2847)
                     /gene="slp"
                     /locus_tag="ACHUPB_004355"
     CDS             complement(2281..2847)
                     /gene="slp"
                     /locus_tag="ACHUPB_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0760983.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          878 a          601 c          668 g          884 t
ORIGIN      
        1 aggttataac ctcagtgtcg aaattgattc gtgacggctc tttcacttta tagttgagga
       61 tattacgatg aaaaaagtat taggcgttat tcttggtggt ctgcttcttc tgccagttgt
      121 gagcaatgca gcggatgcgc aaaaagcagc tgataacaaa aaaccggtca actcctggac
      181 ctgtgaagat ttcctggctg tggacgaatc cttccagcca actgcagttg gttttgctga
      241 agcgctgaac aacaaagata aaccagaaga tgcggtttta gatgttcagg gtattgcaac
      301 cgtaacccca gctatcgttc aggcttgtac tcaggataaa caagccaact ttaaagataa
      361 agttaaaggc gaatgggaca aaattaagaa agatatgtaa ttccgggaat gcgttacatc
      421 gtacttcctt gcatattgaa caggccggaa tatcttcttt aaaagcagct attcctcctg
      481 ttcatatata atctctatat tgaatgggtt acaaaatgaa tatttcatct ctccgtaaag
      541 cgtttatttt tatgggcgct gtagcggctt tgtcactggt gaacgcacaa tctgcgctgg
      601 cagccaatga atccgctaaa gatatgacct gccaggaatt tattgatctg aatccaaaag
      661 caatgacccc ggttgcatgg tggatgctgc atgaagaaac agtatataaa ggtggcgata
      721 ccgttacttt aaatgaaacc gatctcactc aaattcctaa agtgatcgaa tactgtaaga
      781 aaaacccgca gaaaaatttg tataccttca aaaatcaagc atctaatgac ttgccgaatt
      841 aatgaggtac aagtaaaaag gagtagcaag ttgagccatc ttgctgctcc tttttgcatt
      901 tttatatgac agcagaattt attatacgtc ttatactcgc ggcaattgcc tgtggcgcta
      961 ttggcatgga aaggcaaatg cgcggcaaag gagcagggtt acgcacacat gtattaattg
     1021 gcatgggaag cgccctgttt atgattgttt cgaaatatgg ttttgctgac gtgctgtctt
     1081 tagatcacgt cggactcgac cccagccgta ttgctgctca ggtggtgacg ggcgtcgggt
     1141 ttatcggtgc aggtaacatt ctcgttcgta accaaaatat tgtaggtctg acgacggcag
     1201 cggatatctg ggtgaccgcc gccataggta tggttattgg cagcggtatg tacgaactgg
     1261 gtatttatgg ttcagtgatg accttgttgg tgctggaagt cttccatcaa ttaaccttcc
     1321 gcctgatgaa caaaaattac catttacagc tgacattagt gaacggtaat atggtatcaa
     1381 tgctcgactg gtttaagcag caaaaaataa aaactgattt agtttcgttg caggaaaatg
     1441 aagatcatga agtggtcgcc attgatatcc agcttcatgc cacaacgtcg atagaagacc
     1501 tgttgcgatt attaaaaggt atggcagggg tgaaaggcgt ttcaattagc taatctgtgt
     1561 ggcagcgtag ccagactcac cgtaagcctg aaattcacac cagataatca atatgctgat
     1621 ggcgtaccag ctcattgatc cgcttaagat tgaggatcat catgatattg tgtttatggc
     1681 agtagaacgt ttttagcgat attttcagtt tctccagaat atcttcagtt gtcatttgat
     1741 tcgcaatgca gctcagaacc tgatgttgct tgcgcgaaag tgacacattc gcttcaggca
     1801 cagagcagta actgccattg atgagtgcga caaaatcaat gatagtgata gtccgcggaa
     1861 caaaggtcac cggtactttc cccagacagc gtttaatatt aaagggggag aaaacaatga
     1921 catggacagg ttttaatcgt tcaatttgag tgagaaaatt agaatgaaat acattattca
     1981 ttaatgtatc aataatgacg aaggcattct gatgcaacat tacgtcgatc tcttcttcgt
     2041 tctgtatatg aacgacatta cctttactca gaatgttttt catcgcggtg aagaacatcg
     2101 tatccctggt aattataaga aacataaccg actcctggtg cggacattaa ctttataatc
     2161 aatttgccaa aggtaattaa caggacaggg cttataataa taatagttag cttactgtat
     2221 attaatttta tgtaatacat gatgcagatg ctgagaaagc atctgcatct ttcggtggtg
     2281 ttatttgacc agctcaggtg ttacctgact caccgcattg gtgtagtaag gcgcacccca
     2341 gcccggttcc ggccagaatg caccatagcc gtaatcccac aggttatagg tggtattaac
     2401 cacttctctc aaatgccaca cctggatgcc ctgcatattc acttccagga agttatacgg
     2461 gactttattg ataaagccag gttgttcacc ctgaatggta ccgaggatgg taacaaagtg
     2521 attacgatag ttcactgggt caaggaagcc gctttgtctg gcgagcagtc ggccctgata
     2581 gttggcttca atatcaggtt tcgcatagct atccaacggt aatacagcga tttctaacaa
     2641 cgtatccgtt ttgccattga taacgttgat aaccttccca ccaaagcgcg cttgttgacc
     2701 aacatataac cccggctggt tatgaacagc aacaaaactt ttttgaatat caggttggtt
     2761 attgcctttg atattttgcg gaattgaact acatgcggca agcaaaaatg aaaggctgag
     2821 gatgagtgca ccttttgtca tgttcatgtt actatcctta tcaacaaata ttaaaaccat
     2881 aatagatatt tctttatagt tttcatctga ttctgaggcg atatgcctga tagtaacgta
     2941 aacctgtctg atgtttatat cccttttgtt tagttcaatt ccattgattt tggggcgtgg
     3001 catgagtatc catcgcccca taatataatc c
//
LOCUS       DAZOZD010000372         3020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZOZD010000372 DAZOZD010000000
VERSION     DAZOZD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.377"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            94..1695
                     /gene="aceB"
                     /locus_tag="ACHUPB_004356"
                     /pseudo
     CDS             94..1695
                     /gene="aceB"
                     /locus_tag="ACHUPB_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            1725..3016
                     /gene="aceA"
                     /locus_tag="ACHUPB_004357"
                     /pseudo
     CDS             1725..3016
                     /gene="aceA"
                     /locus_tag="ACHUPB_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT          757 a          778 c          827 g          658 t
ORIGIN      
        1 aatgagtagt cttagttgtg ctgaacgaaa agcgcacaac gatccttcgt tcacagtggg
       61 gaagttttcg gatccatgac gaggagctgc acgatgactg aacaggcaac aacaaccgat
      121 gaactggctt tcacaaggcc gtatggcgag caggagaagc aaattcttac tgccgaagcg
      181 gtagaatttc tgactgagct ggtgacgcat tttacgccac aacgcaataa acttctggca
      241 gcgcgcattc agcagcagca agatattgat aacggaacgt tgcctgattt tatttcggaa
      301 acagcttcca ttcgcgatgc tgattggaaa attcgcggga ttcctgcgga cttagaagac
      361 cgccgcgtag agataactgg cccggtagag cgcaagatgg tgatcaacgc gctcaacgcc
      421 aatgtgaaag tctttatggc cgatttcgaa gattcactgg ccccggactg gaacaaagtg
      481 atcgacgggc aaattaacct gcgtgatgca gttaacggca ccatcagcta taccaatgaa
      541 gcaggcaaaa tttatcagct caagcccaat ccagcggttt tgatttgtcg ggtacgcggt
      601 ctacacttgc cggaaaaaca tgtcacctgg cgtggtgagg caatccccgg tagcctgttt
      661 gattttgcgc tctatttctt ccacaactat caggcactgt tggcaaaggg cagcggtccc
      721 tatttctatc tgccgaaaac ccagtcctgg caggaagcgg cctggtggag cgaagtcttc
      781 agctatgcag aagatcgctt taatctgccg cgcggcgcca tcaaggcgac gttgctgatt
      841 gaaacgctgc ccgccgtgtt ccagatggac gaaatccttc acgcgctgcg cgaccatatt
      901 gttggtctga actgcggtcg ctgggattac atcttcagct atatcaaaac gttgaaaaac
      961 tatcccgatc gtgtcctgcc agacagacag gccgtgacga tggataaacc attcctgaat
     1021 gcttactcac gcctgctgat taaaacctgc cataaacgcg gtgcttttgc gatgggcggc
     1081 atggcagcgt ttattccgag caaagatgaa gagcgtaata accaggtgct caacaaagta
     1141 aaagcggata aagcgctgga agtcaataac ggtcacgatg gcacatggat cgctcaccca
     1201 ggccttgcgg acacggcaat ggcggtattc aacgacattc tcggctcccg taaaaatcag
     1261 cttgaagtat tgcgcgaaca agacgcgccg attactgccg atcagctgct ggcaccttgt
     1321 gacggtgaac gcaccgaaga aggtatgcgc gccaacattc gcgtggctgt gcagtacatc
     1381 gaagcgtgga tctccggcaa cggctgcgtg ccgatttatg gcctgatgga agatgcggcg
     1441 acggctgaaa tttcccgtac ctcgatctgg cagtggatcc atcatcaaaa aacgttgagc
     1501 aatggcaaac cggtgaccaa agccttgttc cgctagatgc tgggcgaaga gatgaaagtc
     1561 attgccagcg aactgggcga agaacgtttc tcccagggcc gttttgacga tgccgcacgc
     1621 ttgatggaac agatcaccac ttccgatgag ttaattgatt tcctgaccct gccaggctac
     1681 cgcctgttag cgtaaaccac cacataacta tggagcatct gcacatgaaa acccgtacac
     1741 aacaaattga agaattacag aaagagtgga ctcaaccgcg ttgggaaggc attactcgcc
     1801 catacagtgc ggaagatgtg gtgaaattac gcggttcagt caatcctgaa tgcacgctgg
     1861 cgcaactggg cgcagcgaaa atgtggcgtc tgctgcacgg tgagtcgaaa aaaggctaca
     1921 tcaacagcct cggtgcactg actggcggtc aggcgctgca acaggcgaaa gcgggtattg
     1981 aagcagtcta tctgtcggga tggcaggtag cggcggacgc taacctggcg gccagcatgt
     2041 atccggatca gtcgctctat ccggcaaact cggtgccagc tgtggtggag cggatcaaca
     2101 acaccttccg tcgtgccgat cagatccaat ggtccgcggg cattgagccg ggcgatccgc
     2161 gctatgtcga ttacttcctg ccgatcgttg ccgatgcgga agccggtttt ggcggtgtcc
     2221 tgaatgcctt tgaactgatg aaagcgatga ttgaagccgg tgcagcggca gttcacttcg
     2281 aagatcagct ggcgtcagtg aagaaatgcg gtcacatggg cggcaaagtt ttggtgccaa
     2341 ctcaagaagc tattcagaaa ctggtcgcgg cgcgtctggc agctgacgtg acgggcgttc
     2401 caaccctact ggttgctcgt accgatgccg atgcagcgga tctgatcacc tccgattgcg
     2461 acccgtatga cagcgaattt attaccggcg agcgtaccag tgaaggcttc ttccgtactc
     2521 atgcgggcat tgagcaagcg atcagccgtg gcctggcgta tgcgccatat gctgacctgg
     2581 tctggtgtga aacctccacg ccggatctgg aactggcgcg tcgctttgca caagctatcc
     2641 acgcgaaata tccgggcaaa ctgctggctt ataactgctc gccgtcgttc aactggcaga
     2701 aaaacctcga cgacaaaact attgccagct tccagcagca gctgtcggat atgggctaca
     2761 agttccagtt catcaccctg gcaggtatcc acagcatgtg gttcaacatg tttgacctgg
     2821 caaacgccta tgcctagggc gagggtatga agcactacgt tgagaaagtg cagcagccgg
     2881 aatttgccgc tataccttcg tatctcacca gcaggaagtg ggtacaggtt acttcgataa
     2941 agtgacgact attattcagg gcggcacgtc ttcagtcacc gcgctgaccg gctccactga
     3001 agaatcgcag ttctaagcaa
//
LOCUS       DAZOZD010000373         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOZD010000373 DAZOZD010000000
VERSION     DAZOZD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.378"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUPB_004358"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUPB_004358"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0760984.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUPB_004359"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUPB_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR0760985.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUPB_004360"
     CDS             2805..>2992
                     /locus_tag="ACHUPB_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR0760986.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZOZD010000374         2979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOZD010000374 DAZOZD010000000
VERSION     DAZOZD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.379"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..415)
                     /gene="umuD"
                     /locus_tag="ACHUPB_004361"
     CDS             complement(<1..415)
                     /gene="umuD"
                     /locus_tag="ACHUPB_004361"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0760987.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVI"
     gene            complement(412..660)
                     /locus_tag="ACHUPB_004362"
     CDS             complement(412..660)
                     /locus_tag="ACHUPB_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0760988.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            1054..1980
                     /locus_tag="ACHUPB_004363"
     CDS             1054..1980
                     /locus_tag="ACHUPB_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFR0760989.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLF
                     LAGSAGLLQTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNCVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRLFGCTLEHWYAEQGCDFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            1977..2288
                     /locus_tag="ACHUPB_004364"
     CDS             1977..2288
                     /locus_tag="ACHUPB_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760990.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            2365..>2979
                     /locus_tag="ACHUPB_004365"
     CDS             2365..>2979
                     /locus_tag="ACHUPB_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR0760991.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRL"
BASE COUNT          614 a          809 c          859 g          697 t
ORIGIN      
        1 gtatcacgtg agtgacaaca ccaaaaatac agatgttttc cggatacagt gtgcggaact
       61 ccgggctgtc tgaaaccggc tccagtgccg ggcgtgggcg caacagcagt cgtttgacgg
      121 tgaactcacc gtcgatctca gcgataacga tgtccccgtg ttgtggtttt tcggccctgt
      181 ccactaccag cagatcacca ttctgcacgc cagcctggtt catcgattca ccgctggcgc
      241 gcagaaagaa ggtggctgca ggtctgctga tgcaatagct gttcagatcc agttcctgct
      301 cagcataatc agtggcaggc gaaggaaaac cggcctggca acgatcggca aacaacggtc
      361 gcacgtaact gtcatcgcct gaagggtcag ccggacggtg ataaacggta ctcatgctgc
      421 aggcagccag ctgtcgtcct gccagatttc ttccagcaga gacaggatac gttctttatc
      481 cttgtcgttg cgggctcctg tgacagatac agacggggcg ctgccctggt ctatacgcag
      541 ccagatttcc gggtagtgtg gtttcagacg ttgttcgatt tcattctgta gcgcctgaag
      601 tgtgcctgat ttgaggtttt ttgtgctctg gcggtcgaaa agaatttcaa tgcggatcat
      661 gttctcatct cccttttcta ttgctgtatg catatacagt attctaataa ctgaatccac
      721 acacagtcaa atgtcattat tgtgactaaa aaacgctcaa ctctctgaga gtgtggctgt
      781 atgcctgctg tggactgctg tgtccagctt atccacagca ttttgcgcac ggttctgtga
      841 ataaaatacc tggttaccca ggccgtgccg gcacgttaac cgggctgcat ccgatgcaag
      901 tgtgtcgctg tcgccggcct cctcacccgg tcacgtttcg tcgttcctcc tccacgcgct
      961 gcggcttcgg ggccgcacct gcattcgtat gcggtcgccc ggttacaggt gcggcacggc
     1021 ctgatggagg ccgcatgtga gaggagaatt cccatgccaa actggtgctc gaatcgtatg
     1081 tatttttctg gtgaaccggc acagatcgct gagattaaac gactggccag cggcgcagtc
     1141 acaccgtttt atcgccgcgc cacaaatgaa ggtattcagc tgtttctggc cggaagtgcc
     1201 ggacttctgc agaccactga agatgtgcag tttgaaccgt gccccggact gacggctgcc
     1261 ggacgtggtg ttgtatcgcc ggagaatatc gcgttcacac gctggctgac acacctgcag
     1321 aacggtgtgc tactggatga acaaaactgc ctgatgctgc atgaactctg gctgcagagc
     1381 ggtactggac agcgtcgctg ggaaggatta ccggacgatg tcagggatac catcaccgca
     1441 cttttcaccg caaaaagagg tgactggtgt ggcttctgga gtaacgagga tgtatcggtg
     1501 tggtggaacc gtctgtgtga caacgtactg ccggaaaaaa ccatgccgtt tgacctgctg
     1561 acggttttgc cgacccgcct ggatattgaa gtgaatggct ttaacggcgg tgttctgaac
     1621 tgtgttcctt ctgcatatca ctggtataca gaacggtatg gcgtgaagtg gccttgtggg
     1681 tatgacctga atatcagcag tcagggggaa aacttcattc aggtagattt cgacacgccg
     1741 tggtgtcagc cggaaagcga cgttgttgca gaattaagcc gccttttcgg ctgcacgctg
     1801 gagcactggt acgccgaaca gggctgtgat ttctgtggct ggcagttgta tgagcgcgga
     1861 gagcttgttg atgtgctgtg gggggaactg gaatggtctt ccccgacaga tgacgatgag
     1921 ctgccggaag tcaccgggcc tgcgtggata gtcgacaatg tggcgcatta tggcggatga
     1981 agtatgacgg agacgggcgg gcaaccgccc gtttcttttc cggtaaagga tgtcgccgtg
     2041 ctcctctttt tactgcgccg gctgacgcgg cgcggcataa acgctgcctg tggtcagtgt
     2101 cccgcgtccg gtgggcacgg gacgggcgct tttaccgctc ccggctggcg tcggtgacag
     2161 tgtacgccag cctgtcgccc ttttctgatg aacgcccttc aagccgcttt cgcggcatag
     2221 ccttgccgtc agaaagacga cggctgcggt attccacggt cggcctgacc cgttaccgga
     2281 cgcggtgaac agcccacagg cagcggggaa cgggcaccac aggggtgccc tcccggtgtc
     2341 ctctgtaaga gaaggagcgt tgttatgtcc cgttttgtcc tcggtaactg catcgatgtt
     2401 atggcccgta ttcctgataa cgccattgat ttcatactga ctgacccgcc gtacctcgtc
     2461 ggttttcgtg accgttccgg gcgcaccatc gctggcgata aaaccgatga atggctgcaa
     2521 ccggcctgta atgaaatgta ccgcgtactc aaaaaagacg cgctgatggt cagcttctac
     2581 ggctggcacc gtattgaccg ctttatggcg gcctggaaac gcgccggatt cagcgtggca
     2641 gggcacctcg tgttcaccaa aaactacaca tccaaatctg cgtatgtcgc gtaccgccac
     2701 gaatgcgctt acatcctggc aaagggcagt ccggcccggc cccgtaaccc tctgccggat
     2761 gtgctgggct ggaaatattc aggcaatcgt catcacccga cggaaaagcc cgttaccagc
     2821 ctgcaaccac tgattgagag cttcacacat ccgaacgcga ttgtgctgga cccgtttgca
     2881 ggcagcggct caacctgcgt cgccgccctc cagtccggac gccggtatat cggtatcgag
     2941 ctgcttgaac agtatcaccg tgccggacag caacgactg
//
LOCUS       DAZOZD010000375         2958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOZD010000375 DAZOZD010000000
VERSION     DAZOZD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.380"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            776..1665
                     /locus_tag="ACHUPB_004366"
                     /pseudo
     CDS             776..1665
                     /locus_tag="ACHUPB_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1665..1955
                     /locus_tag="ACHUPB_004367"
     CDS             1665..1955
                     /locus_tag="ACHUPB_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760992.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2276..2566
                     /locus_tag="ACHUPB_004368"
     CDS             2276..2566
                     /locus_tag="ACHUPB_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760993.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2566..>2958
                     /locus_tag="ACHUPB_004369"
     CDS             2566..>2958
                     /locus_tag="ACHUPB_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760994.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYI"
BASE COUNT          990 a          564 c          599 g          805 t
ORIGIN      
        1 tttagtagct ttcctgatac cgcatagagc caacaccagc tactggctta ggcacattta
       61 cagcaataac cactgtcatg aaatccgtat tcttcatcgt gcagttaaat accttccacc
      121 agcagggcat aacaggctta ctattaggtc accgtttcca agtgcagtcg ttgtttttaa
      181 aaaagaacca cgtaagcatg aaattacaca gatggtttgc tgtgctgata cattgctacc
      241 gttaaccatc attaaccgtg gtggactaag aggaagaccc accatttacc cacctgagac
      301 gctcgacagc ttcataaaac tatacaggca aggaaaaccc attaagtaca ttgcagatgc
      361 tctcagaatg cctctgagca cgtcatacag gattgctcaa aggttgagct aaaattctca
      421 taaatatggt catttacaag acaggtgatt cagatgttaa gaagtatccc tgtgaacaat
      481 aaatgaccaa tcagtaatca ttctgtggaa taattgttgc aatttataat cgcatctgtt
      541 atagtttcta tatgggctga taaaaaagtt catgtcttga agtggtaaca cttggggagg
      601 tgtcagatgg ttggcgtata ttaacctctc attcagggta tacctcagcg ttttaatcat
      661 cgtactgttt caggcatgga cttctttatc agtccatcaa tcccccagat attttaatca
      721 cacaggctaa tcggttttat ccggttggcc ttttcacgta tgaggtaatt tatgaatgtc
      781 taaatataac ccaaatcagt tttataaatt tgatgtgcga atctctcaac tttcagaaga
      841 aattaacgca gcagcaagat tgatttatat ggtcatggtc catagctgtg acttctatga
      901 agagaaatac aaatcatatt caccaagtat tgaccatctc gcttatatgg ctggtgtatc
      961 aaagagaaca gcaatcagag ccattgagac acttgagaaa cacggtttaa ttcaggtgca
     1021 aaagaatcag gggcgtcaaa gcacttatcg tgtattcaca ctggatgata agccggaatt
     1081 acttgtaaga cctacatcac aggaagttgc agcctcagta agacaaaatg acaccactac
     1141 cagtgacaaa atgtcatggg ggtagtgaca aaatgtcacc cgtgttagag tttagataag
     1201 ttattagata aaacattaga taataatatt acagataaga ataaagagac tggacttaat
     1261 gatgatatta aagaagatga ctttctttct ttggatgaat taaatgattt tctctctatg
     1321 aatgaatctg accttgaagt aagctctaaa aagacttcta aacaagacag agaaatcact
     1381 gatgtgagtg ttgatagtga ccataaaaac acacagtgcc ccgccactga tgatgagttg
     1441 gaccccttcg atgatgaaga ggatgaacgt gaaaaagcca ttaagagggc agagaagaaa
     1501 aagagattcg agcttgcaag acaaaagaac aaagtatcgg gtttagaccc attcgatgac
     1561 agtgaagatg attattatct ttcaccgaaa aagaaacaaa ctcaggatga caagaagcaa
     1621 aaaattaaac cgaagatgta ttttgccggt ggaggaatta agtaatgaaa gaatatcacg
     1681 gtgaaaagag atacaaagat tacctgttaa agcgttacag catttctcgt gaaggtcatc
     1741 ttatgaagga tactcacggg gaagtttacc ggattagacc aaagaaagaa gggaggggtt
     1801 atttctttta cgatggtgtg acagatttaa agattgacgc tttgaggttt gcagtgctgt
     1861 atcattttga tatctggaat tcaatccacc agctacgatt aaaagacggt gaccccagca
     1921 acttaaaggc caccaatatt atcacaaaac gttagtttgt tttaccatca tcaatcgtaa
     1981 ttgtgatttc atatttgaaa agcttaatgc tgaaattgaa tcgtgtactt ctggcgataa
     2041 tcatttttaa aagctgatac acaagaagca ccagaaacac aaagcaacct aagcttgcga
     2101 atctgtgaga ttcagcaatt gtttcaagaa agcccattaa ttccccttat ttatcaaagg
     2161 gaatgacctg tatcagcccc ctacaaccat attaacatgt aaatgagttg ttgacaatat
     2221 gttataaata tgttaaagtt agttataagt caaagatttt aagaaggaaa atttaatgag
     2281 acacacagtt acacgccttt tgccagaggc atattccaga ctggcagcaa tcaaagacaa
     2341 atacccagcc atgagccaca cagcacttgt tggtgagatt ttgaaggaac acatagccct
     2401 tacaccgttt gacgctatca agggcgttgc agagggttct aaggccatca gaatcacaac
     2461 gggccaactg gaactactca aagcacagtc cgcagtggca ggttgctcaa tgattcaggt
     2521 gttgagtgcc aaggttcatc aactggcgga taagctgggg gtttaatgct atgcacattt
     2581 gattatcatg cgttttatcg ccatagaggt caccttgtcc gtgagaataa cggaacatgg
     2641 aaagatttaa ctcgtaatgc tgaacccttc acacaccagc aattaaaatc ccgcttctcc
     2701 agatggaaga atgcaccagc agatactggt gattttgaga ttatccatac cttcgattat
     2761 gcctcagaag atgaccttca cgatgcaatt aagctgattg aagacttcaa tacctcagaa
     2821 gatgtggatg gtgacaaata taaatcccgt ggtaaccgct ataaccgtga tgatatcact
     2881 caggccataa atgctggtca atctcagcga tacattacag aaaaatatgg tatttcacgg
     2941 agacagtatt tctacatt
//
LOCUS       DAZOZD010000376         2944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOZD010000376 DAZOZD010000000
VERSION     DAZOZD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.381"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            45..1120
                     /locus_tag="ACHUPB_004370"
                     /pseudo
     CDS             45..1120
                     /locus_tag="ACHUPB_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1136..1884
                     /locus_tag="ACHUPB_004371"
                     /pseudo
     CDS             1136..1884
                     /locus_tag="ACHUPB_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1886..>2944
                     /locus_tag="ACHUPB_004372"
     CDS             1886..>2944
                     /locus_tag="ACHUPB_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760995.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAZOZD010000377         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOZD010000377 DAZOZD010000000
VERSION     DAZOZD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.382"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..267
                     /locus_tag="ACHUPB_004373"
     CDS             <1..267
                     /locus_tag="ACHUPB_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760996.1"
                     /translation="ITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLWFELVT
                     KIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(458..724)
                     /locus_tag="ACHUPB_004374"
     CDS             complement(458..724)
                     /locus_tag="ACHUPB_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760997.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(724..1200)
                     /locus_tag="ACHUPB_004375"
     CDS             complement(724..1200)
                     /locus_tag="ACHUPB_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0760998.1"
                     /translation="MCDSEAEKILFILDKHERAYRCQYPVYIFLAIVLWILPILLIIY
                     LHFCIGIGFGIAWTAYYFLSLFEYFVKYFFPLDREDLLNLMDLAKDSPDAKQELLERL
                     LSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDKKGIR"
     gene            complement(1256..1858)
                     /locus_tag="ACHUPB_004376"
     CDS             complement(1256..1858)
                     /locus_tag="ACHUPB_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR0760999.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            2212..>2939
                     /gene="yagA"
                     /locus_tag="ACHUPB_004377"
     CDS             2212..>2939
                     /gene="yagA"
                     /locus_tag="ACHUPB_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0761000.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIKLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARR"
BASE COUNT          793 a          631 c          631 g          884 t
ORIGIN      
        1 ataacgaaaa ttaccgctga acgtattagc gactacatga ccgaagcagt taatacagac
       61 tctatagccg ttgcggaaat gtttcataac gctgcctggg gagtgcgaac attgtggttt
      121 gaactcgtca caaaaataga tatagatata cataaaaaga acaggtatgc gagttatgat
      181 cttagaagga aaatcgaaat gcagcatgaa gagtttcaga aaatgacaga gcgcgagcaa
      241 gtgcctttat tgaaatcgcc agaataatag aaacagtaca agtacattgc tgtattgtgt
      301 tttccttgaa tattgaatcg cggtgcccat gtactacgat tcctttcgtg ttgtttatca
      361 ttgcacactt tccctgactg agcgtaaaca ctgactactt tcccctcaaa ctcctggacg
      421 gcgaatgcaa aattggtctg ttccagcata gaatatgtta ctttttcatg accgaacccg
      481 aaattttgcg cgtcaataag agacgttgtg taaactcagt ccttttatct ggcaggttaa
      541 cccctatcaa tcttaggatg aaaccacgcc atcctctgtt tgcctggtta ttaatttcat
      601 aaagacgatt gttttcctct cgtagttttg tccactctaa acaaagtgtt atatcatcca
      661 gttctgtgat gtgtttcact tttccattga cagttacagt gtttattcca tttcctgtga
      721 tcatcatctt atgccttttt tatcttcatt tgggaccgtg cttttcttcg gagtatcact
      781 attcgttaac tgctgtttat caataaaatt cctgatgtta tcaagtgctt cctttgtttt
      841 tagaatgcgc ttgatatcca tttcatcccg cccggtcatt ctttttccgg acaaaagacg
      901 ttcaagaagc tcctgtttcg catcaggaga atctttggcg agatccatca ggttaagcaa
      961 atcttcacga tcgagaggaa agaagtattt tacaaaatac tcgaaaagcg aaagaaagta
     1021 gtatgctgtc catgcaatac cgaaaccgat tcctatacag aaatgcaggt atataatcag
     1081 gagaataggt aaaatccata atactatagc taaaaaaata tatacggggt actggcatct
     1141 gtatgcccgt tcatgtttat ccaggatgaa taaaatcttc tccgcttctg aatcacacat
     1201 aacattctct tcccattttt tctgatacgt aatgtccacc tgttatcagg agtgtttatt
     1261 gcccagaaat attctcctgc tgtgcctgca tcctgagcca cttttgttct aatgtcgcca
     1321 gtctgcgacc ggcactctgt gcttcagagt ctgagacctg ccctttgggg tgaccatcca
     1381 ggccataacg tgggacgccg gcgacagctg tccgctgcag atatacgcgg cgggatgtga
     1441 tgcaggacac tgcacagagc cattcgtcca tggtcagttc tgtctccggg tgttctgcca
     1501 gaaatgccga aatatcattg tttatgccaa ttttcagggg aataaatcca ccaccttcag
     1561 cacgaagatg aggaaacagg gcctttattt tttccacgca ctcgtgagta tactcacggg
     1621 tccagtggga gggacgtgga aaacggtgtc ttttcttttt cttcttcctg gctggcgctt
     1681 cggggatatg tgtttcgacg gcagatgaat ttattccggc gggggctgat tctgccgtct
     1741 gttcagtaaa tacaggtgcg ataatatctg ttttttcgga taaggacggt ggcgaaaaag
     1801 tacgacgttt tttcaccaca atgacgggag ttttcctttc ttcctgattt tttcccataa
     1861 atacctcggc tggctggttt tactctttaa taatatcaca attcataaat tagtaaattt
     1921 cccatcaggt gatttttcat ggctaataag tccgttctct gagtatttat actcgaggta
     1981 gtaacagtta tggcgtgaca gtcgaattga tattctactg aaccctgaca gaaacatggt
     2041 tgatatattc tctcacttta atgttgtcat gatataaata aaaacaattc atttgaaagg
     2101 atatcagaag aagatatcgg ggcgcacagt tcatctctgc ctttgtttca tggggggttc
     2161 tttgtatatt ttacttactg tttaagcatg tacataagga gtgctttcat tatgccaatg
     2221 atacttgtaa agaagaatcc ccggggcaaa gtgattcggg agcttagcag tgaggaagag
     2281 actgcagtaa agacggtctg tggactgaaa aagccagcaa ccatggcact acataacctg
     2341 gccaatgata ttctccggga aatgcgtgag tatgatgcat ggttacagtg tgactgtatt
     2401 ccgggagatt cgccggccat gaattttgct gctcttaaaa acaatacggg tactctttat
     2461 ctgtccagtt ttaatcatga gcatgcacct gaatgcccca tgtatcgtca gttatccgga
     2521 aatgaagagg aaacatcttc cggagcaagc cggcatcccg ttagcacccg tataaattac
     2581 agaaactttt taccacctga tgacagtaat tctgttatca aactgcaggc ccgctctgct
     2641 taccataatg gtgagagctc ctccgttcgc aagaaacgtc cgcgccttgc ccgtttactc
     2701 ctgtcgctta ttgacgatgc tgggctgaat aaactggatt ccctggctaa tccccgtatc
     2761 cggactaatt atcgtgagtg tcttgatgcc atacggcagg ttacactgca gcaggagtat
     2821 attcggggaa gatccttatc tgaaattatc catttccggc ctggaatgtc tgaaaggtct
     2881 caggagcggt taatggaaac gctggagaat tcagaacgac actggcctgc cagaagaaa
//
LOCUS       DAZOZD010000378         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOZD010000378 DAZOZD010000000
VERSION     DAZOZD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.383"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUPB_004378"
     CDS             37..264
                     /locus_tag="ACHUPB_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0761001.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUPB_004379"
     CDS             complement(265..639)
                     /locus_tag="ACHUPB_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR0761002.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUPB_004380"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUPB_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUPB_004381"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUPB_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0761003.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZOZD010000379         2933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOZD010000379 DAZOZD010000000
VERSION     DAZOZD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.384"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(116..192)
                     /locus_tag="ACHUPB_004382"
     tRNA            complement(116..192)
                     /locus_tag="ACHUPB_004382"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Asp,seq:gtc)
     gene            complement(325..1056)
                     /gene="dnaQ"
                     /locus_tag="ACHUPB_004383"
     CDS             complement(325..1056)
                     /gene="dnaQ"
                     /locus_tag="ACHUPB_004383"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0761004.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1121..1588
                     /gene="rnhA"
                     /locus_tag="ACHUPB_004384"
     CDS             1121..1588
                     /gene="rnhA"
                     /locus_tag="ACHUPB_004384"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0761005.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1585..2307)
                     /locus_tag="ACHUPB_004385"
     CDS             complement(1585..2307)
                     /locus_tag="ACHUPB_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0761006.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2341..>2933
                     /gene="gloB"
                     /locus_tag="ACHUPB_004386"
     CDS             2341..>2933
                     /gene="gloB"
                     /locus_tag="ACHUPB_004386"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0761007.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRA"
BASE COUNT          789 a          717 c          689 g          738 t
ORIGIN      
        1 aacacattta cagcataaca aaaccataga ccgaagaaat agcgggattt aaatgcaaaa
       61 aaaccgcaaa attaaaaatc ttgcggttct ctgaactcat tttcatgagt gaatttggcg
      121 gaacggacgg gactcgaacc cgcgcccccc tgcgtgacag gcaggtattc taaccgactg
      181 aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcacc
      241 cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagccgct
      301 atttttagcg ccttttacag gtatttatgc ccgccagagg cagcttccgc ccttcttctc
      361 caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg
      421 taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgctgtgt
      481 ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc
      541 cgcaaggatc tgtgcatcga gtaatgcccc gtgtagcgtt cgtttactgt tatctatttc
      601 gtagcgggca cataacgcat cgaggctgtt gcgcttacca ggaaacattt tcctcgccac
      661 cgcaaggcta tcggtgacct tacagaaggt attggtcttc ggaatatcgc gcttaagcaa
      721 cgaaaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc
      781 gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa
      841 aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt
      901 gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat
      961 ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt
     1021 atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttat
     1081 cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat
     1141 tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg ggttacggcg ctattttacg
     1201 ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat
     1261 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt
     1321 gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa
     1381 acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat gtcgatctct ggcaacgtct
     1441 tgatgctgcc ctagggcaac atcaaatcaa atgggaatgg gttaaaggcc atgccggaca
     1501 cccggaaaac gaacgctgtg atgaactggc tcgtgctgcg gcaatgaatc ccacactgga
     1561 agatacgggc taccaagtcg aagtttaagc ttgtggttta cgacattgcc gggtggctcc
     1621 aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcgggttta gcgttaaagg
     1681 aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt
     1741 caatagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgcagcac
     1801 ttcaaaattc aacaaagaga gccagtccag ttgccgcatc agagtaaaca tccggctgtt
     1861 atagggcgag gtttttcgca ataccggcac aagtttgcgt aatcccatta aactaatggg
     1921 attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcgg tttcacgcaa
     1981 taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga
     2041 tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc
     2101 agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt
     2161 gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc
     2221 cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac
     2281 agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactaa gaggtaatct
     2341 atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa
     2401 gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc
     2461 gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc
     2521 ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca
     2581 caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat
     2641 gaatttagtg taattgccac accgggtcac actttaggac atatctgtta cttcagtaaa
     2701 ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtaggtt gtttgaaggg
     2761 acagcatcac aaatgtatca atcacttaat aagttaagtg cgttacctga cgatacattg
     2821 gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg
     2881 cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc aaa
//
LOCUS       DAZOZD010000380         2877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOZD010000380 DAZOZD010000000
VERSION     DAZOZD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.385"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUPB_004387"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHUPB_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0761008.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUPB_004388"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="ACHUPB_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0761009.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="ACHUPB_004389"
     CDS             1649..2098
                     /locus_tag="ACHUPB_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0761010.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUPB_004390"
     CDS             complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHUPB_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0761011.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUPB_004391"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHUPB_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0761012.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUPB_004392"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHUPB_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0761013.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAZOZD010000381         2856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOZD010000381 DAZOZD010000000
VERSION     DAZOZD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.386"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..592
                     /locus_tag="ACHUPB_004393"
     CDS             <1..592
                     /locus_tag="ACHUPB_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR0761014.1"
                     /translation="TLTGNNKKNEPHDKGWGILEQGTIALVVLFVIVVVLGSLYALRT
                     RTNVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGAVPSGMTVQGDKTS
                     GTATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTGLTNGITLN
                     STAHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            602..1162
                     /locus_tag="ACHUPB_004394"
     CDS             602..1162
                     /locus_tag="ACHUPB_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR0761015.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            1147..1803
                     /locus_tag="ACHUPB_004395"
     CDS             1147..1803
                     /locus_tag="ACHUPB_004395"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR0761016.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            1803..>2856
                     /locus_tag="ACHUPB_004396"
     CDS             1803..>2856
                     /locus_tag="ACHUPB_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR0761017.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDN"
BASE COUNT          817 a          608 c          680 g          751 t
ORIGIN      
        1 cactttaact ggaaataata agaagaatga gcctcatgat aaaggatggg gaattttgga
       61 gcagggaaca atagcgctcg ttgttctttt tgttatagtc gttgttctgg gtagtttgta
      121 tgcgttacgt acaagaacca atgtggcaac tgaaaccgca aatatacaaa caattatcac
      181 aagtgcccag agcctgctaa aaggtagcga tggatataca tttaccagca gtgccaaaat
      241 gacaggtgcc ctcattcaga tgggggccgt tccctctggg atgacagtac agggcgacaa
      301 aacatctggt acagcaacgc tctataacgc atggggcggt gctgtaactg ttgccccggc
      361 ttcaacatca gggttcaaca acggttttac cgttacctat gataaagtcc cacaggatgc
      421 ctgtatccag attgccacac ggatcagtaa aactggttta actaacggca ttaccctgaa
      481 cagtactgct cacagtgatg gaaaagtcac tactgaagaa gccagtacgc aatgcaaggc
      541 agataatggc agcactggta caaacaaact tatcttcacc atcaatggtt aaggagtttt
      601 aatgtcaggg ggtacagtac ccctcagact tatttcatta atcctgcttt tttctcttat
      661 tccacccgcc cgagcttcat acgtagcccc caaacagatc tggaccaaca agtgggacaa
      721 ttgctttgcc gctgccggcg cgcgctatca aattgaacca ttattattga aggctatttc
      781 tgccggggaa tcttcgttga ggccgggcgc aattaatata aataaggaca aaaaaacagg
      841 taaagccagt agcacggatt atgggttgat gcaaataaac tcaacccata ttccaaagct
      901 gataaatatg ggcgtaataa aaaagtctga ggatttaatc accaaaccat gccttaatat
      961 ccatatagga agctggatac tggcaagaca ttttcagata tgtggcgtca gctggaattg
     1021 ccttgggtca tacaatgccg gattccgtaa ggacagacat gaaacaagag agcaatatgc
     1081 caataaaata tggagaattt atcgtgacat gaaagggata tgtcttcccg gacagggagg
     1141 caggcaatgc agacaatcgt aggcataagt ctatcccctg tttatatact gccattgatg
     1201 ttcacatttt ccattattgg acgaactctg ctattcagag ttcgttattt tttatctgat
     1261 acgggacatc tctggtataa gacacaccct gctgttctgt caggaatatg gctatacagt
     1321 atagctgtgg cgttaattat tttatcatca tcaccactgc tctaccgtat tcatgctgtc
     1381 cttattcttt cgttcatact gcaaatggcg gtaacggatg cactgacagg tttattacca
     1441 ggaacattca cccggcgttt tttaattgca ggaatgctat cacaaatcac gactgatata
     1501 tggtggttcc gtacaacaga atttgccact gctgcaattg tcttattctg ccttcataaa
     1561 ttagttaacc gccatcgtct taatattggc actggagacc tgtggcttat tgcaggtatt
     1621 accgcatggt cagggttata taacgccata tggtgtgtgt tgctgggcac aggaggattt
     1681 gtcttatggc attcaacatg gtgcataaaa gggcataaag aaggccctct gggtccatgg
     1741 ctatgttttg gccatgtcct tttgctgctg gataatcttt atcaaccact atgggtaatc
     1801 tgatgaaaaa atatgaccga ggctgggcat cgctggaaac tggcgcggct ttactgattg
     1861 ttatgctcct gattgcctgg ggagcgggca tatggcagga ctatattcaa acaaaaggat
     1921 ggcaaactga agcacgtctt gtcagcaact ggaccagtgc ggcccgctct tatataggga
     1981 aaaactacac gactctccag ggcagtagta ccacaacaac tcctgccgtt atcacgacaa
     2041 ccatgctgaa aaataccggc tttttgtcca gcgggtttac tgagacaaac agcgaggggc
     2101 agcggttaca ggcatatgtg gttcgaaacg cccaaaaccc ggaattacta caggcaatgg
     2161 ttgtatccag tggtggcaca ccttatccag tgaaagcact tatccagatg gctaaggata
     2221 ttaccactgg tcttggtgga tatatccagg acggcaaaac agccacaggt gcattacgtt
     2281 cctggtcagt agctttaagt aattatggtg ccaaaagcgg taacgggcat attgccgtat
     2341 tgttatcgac agatgaactt agtggtgcag ctgaggacac tgatcgtctt tacagattcc
     2401 aggtcaatgg tcgccctgac ttaaacaaaa tgcacacggc cattgatatg ggatcaaata
     2461 acctgaataa cgttggggca gtaaatgccc agacaggtaa tttcagcggc aatgtgaatg
     2521 gtgtaaatgg cactttcagc ggtcaggtaa aaggcaatag cggaaacttt gacgtaaatg
     2581 tcaccgctgg cggtgatatc agaagtaata atggttggtt aattactcgt aacagtaaag
     2641 gctggctcaa tgaaactcac ggtggcggat tttatatgtc cgatggatca tgggttcgaa
     2701 gtgtaaacaa caagggcatc tataccggcg gtcaggtgaa aggcggtact gttcgggctg
     2761 atggtcgcct ttatactggt gaatacttac aactggaaag aactgccgtt gctggcgcat
     2821 catgttcgcc taacggcctt gtaggccgcg ataata
//
LOCUS       DAZOZD010000382         2849 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOZD010000382 DAZOZD010000000
VERSION     DAZOZD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.387"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..361
                     /locus_tag="ACHUPB_004397"
     CDS             <1..361
                     /locus_tag="ACHUPB_004397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761018.1"
                     /translation="TEYYSIFDVVCGILDRLKKFGDLFYWKRRKKQHKRSFVKLFIFL
                     TEFKKTTLFRGLKRATVTLRNRVGFSKRWKRGILRIVIQWLLGSSRSGKPENGRMRHK
                     AFRTYGRNDGSLPGPEG"
     gene            546..1418
                     /gene="repA"
                     /locus_tag="ACHUPB_004398"
     CDS             546..1418
                     /gene="repA"
                     /locus_tag="ACHUPB_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR0761019.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            2322..2606
                     /locus_tag="ACHUPB_004399"
     CDS             2322..2606
                     /locus_tag="ACHUPB_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFR0761020.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            2606..>2849
                     /locus_tag="ACHUPB_004400"
     CDS             2606..>2849
                     /locus_tag="ACHUPB_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0761021.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYV"
BASE COUNT          725 a          754 c          752 g          618 t
ORIGIN      
        1 gacagaatat tattctatat ttgatgttgt gtgcgggata ttagatcgtt taaaaaaatt
       61 cggcgatctc ttctactgga aaagaagaaa aaagcagcat aaaagatcgt ttgtgaaatt
      121 gttcattttt ttgacagagt tcaaaaaaac gacgcttttt agggggttaa agagggcaac
      181 tgtaactctg agaaatcgtg tcggcttttc aaagcggtgg aaaaggggta tattgcggat
      241 cgttattcag tggcttttgg gatcctcgcg gtccggaaag ccagaaaacg gcagaatgcg
      301 ccataaggca ttcaggacgt atggcagaaa cgacggcagt ttgccggggc cggaaggctg
      361 aaaaagtttc agaagaccat aaaggcgaaa cccccatcat ctttcctcga aacttggcgg
      421 aatcagagaa agataatggg ggtatcacgg aatacgggac aagtatatat gaaaccgtgt
      481 cagagattca acccttcgca gtgcattaac gcacggcaat ctgttcccgc cataagcgac
      541 agcttgtggc ggccctgaac cagacctcat tcactgaaag tacataccgt caggtaaaga
      601 acccgaatcc ggtgttcaca ccccgtgaag atgccggaac gctgaagttc tgcgaaaaac
      661 tgatggaaaa ggccgtgggc ttcacctccc gttttgattt tgccgcgcat gtggcgtatg
      721 cccgttcgcg cggcctgcgc cggcgtatgc cgccggtgct gcgccgccgg gcgattgatg
      781 cgctgctgca ggggctgtgt ttccactatg acccgctggc caaccgcgtg cagtgctcca
      841 tcaccacgct ggccattgag tgcggactgg cgacggagtc tgaggccggg aaactctcca
      901 tcacccgtgc cacccgtgcc ctgaagttcc tgtctgagct ggggctgata acctaccaga
      961 cggaatacga cccgacaatt ggctgtaaca tcccgaccga catcacgttc acgccggcac
     1021 tgtttgactc actcgatatc tcagaagagg cagtggcttc tgcacgccgc agccgcgtgg
     1081 aatgggaaaa caggctgcgc aaaaagcagg ggctggatgc gctgggtatg gatgaactga
     1141 tagcgagggc ctggcgtttt gtgcgcgagc gtttccgcag ttatcaggca gagcttaagt
     1201 cccacggcat gaaacgtgcc cgtgcgcgcc gcgatgcagg caggacccgt caggacattg
     1261 tcaccctggt gaaacggcag ctgacacgtg aaatcgcgga agggcgtttc cgcggaagcc
     1321 tggaggccgt gaagcgcgaa atcgaccgtc gcgtgaagga acgcatgata atgtcgcgga
     1381 acaacaacta cacacggctg gccactgcct caccctgagt ttgacctcat ctgaataatc
     1441 cggcctgcgc cggaggcttc cgcacgtctg aagcccggcg acgcacaaaa aagcagcaca
     1501 gcacacaaaa aacagcctca tcatctgcct tccggctcgc cttgctcccc ctgtttttca
     1561 cgcaaaacac gtctcactga cggggaattc tgtttatcca catttaactg caagggactt
     1621 ctccgtaagg ttacaaccgt taatgctata aagcgccagc cgcaggcgtt acagggtgca
     1681 atgtttcttt taaaccccag tactgtattt cctttaaact acttaattac ttccatttaa
     1741 aaaaaaagac ctattcaatg cctgtcctgt ggacagacaa atatgcacct cccacgctga
     1801 cgcgcgggcc ccgaacctca ccgttctgaa accacaacaa aaagacatca ggaataaaaa
     1861 caccacacaa acgcagcacc gtgcctaccc ctcataactg aaaagcgagg ccgcccccgc
     1921 ccgaagggcg ggaacaacat cgcttttaat tatgaatgtt gtaactacat tgtcatcgct
     1981 gccagtcttc tggctggacg tatcgagtac acgctcgtaa gcggccctga cggcccgcta
     2041 acgcggagat acgccccgac ttcgggtaaa ccctcgtcgg gaccactccg accgcgcaca
     2101 gaagctctgt catggctgaa agcgggtatg gcttggcagg gctggggatg ggtaaggtga
     2161 aatctatcaa tcagtaccgg cttacgccgg gcttcggcgg tttcacacct gtgctacatg
     2221 taaccaccgt atgccgcctt acatgcgctg acgcgcatta tttgccttgt ttcgtctgaa
     2281 agtaatcact atgattaaat atgattaaca actaatcgga tatgcaaatg aaaaacaata
     2341 ccgcacaggc aacaaaagtc attaccgcgc atgtgccatt acctatggct gataaagtcg
     2401 accagatggc cgccaggctg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
     2461 catggcttgc ccaggaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
     2521 catccggaca ggttatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     2581 acaatccgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cgctttctga
     2641 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgcggccg ccagaacggt
     2701 gcaatccctg acacaggcac cggtcattct gttaactcat ccacgtatgg gagaacagtt
     2761 gtttcagttt gaacccaggg aggtcagacg gatttttgct ggcgagtacg aaatccgtta
     2821 cgaacttaat ggccagacta tttatgtat
//
LOCUS       DAZOZD010000383         2739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOZD010000383 DAZOZD010000000
VERSION     DAZOZD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.388"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHUPB_004401"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="ACHUPB_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR0761022.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="ACHUPB_004402"
     CDS             1322..2374
                     /locus_tag="ACHUPB_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR0761023.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAZOZD010000384         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOZD010000384 DAZOZD010000000
VERSION     DAZOZD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.389"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUPB_004403"
     CDS             complement(423..680)
                     /locus_tag="ACHUPB_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761024.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUPB_004404"
     CDS             complement(677..1174)
                     /locus_tag="ACHUPB_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR0761025.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUPB_004405"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUPB_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0761026.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUPB_004406"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUPB_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0761027.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUPB_004407"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUPB_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR0761028.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          650 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZOZD010000385         2723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOZD010000385 DAZOZD010000000
VERSION     DAZOZD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.390"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            30..2546
                     /locus_tag="ACHUPB_004408"
     CDS             30..2546
                     /locus_tag="ACHUPB_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0761029.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2723
                     /locus_tag="ACHUPB_004409"
     CDS             2557..>2723
                     /locus_tag="ACHUPB_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761030.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          658 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga gtt
//
LOCUS       DAZOZD010000386         2695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOZD010000386 DAZOZD010000000
VERSION     DAZOZD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.391"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            19..573
                     /locus_tag="ACHUPB_004410"
     CDS             19..573
                     /locus_tag="ACHUPB_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0761031.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(583..2010)
                     /locus_tag="ACHUPB_004411"
     CDS             complement(583..2010)
                     /locus_tag="ACHUPB_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0761032.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2007..2693)
                     /locus_tag="ACHUPB_004412"
     CDS             complement(2007..2693)
                     /locus_tag="ACHUPB_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0761033.1"
                     /translation="MLFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDDRD
                     DLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECC
                     GEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGYTF
                     GRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQPLADYPTC
                     PETLKDMSDL"
BASE COUNT          772 a          649 c          605 g          669 t
ORIGIN      
        1 aaatcgaagc attcacacat gaataaaaaa tcaatgtata tattcgcaat ttttatagtt
       61 gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa agccatggtg
      121 aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac ggcagccacg
      181 gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt
      241 catggtagcg acggtaaacc actggattat acacaggcga gatactggta tgagcaatca
      301 gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg
      361 ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg
      421 tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt
      481 aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg
      541 gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga agagatggag
      601 tatttttcct atcaaaccgg caaagataaa aatgtagaaa ataattttga tgacgcccag
      661 acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg gctcgacggt
      721 ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag
      781 tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt cttctgtcgg
      841 ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt
      901 atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga
      961 acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg
     1021 ggcgctgtcg ccggggacat aaagtagctg tttaccctga tcgttaacca gttcacgagc
     1081 ttcaatcatc atcaccacta acgggtaata atcgacccga tgctgacggc accattgcaa
     1141 tattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa gttctgccag
     1201 caagctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataac gcgcatcttc
     1261 attatcaggt tcagcttccc gccacgaccg ggcgtaatcc agaagttcgg ggatatccag
     1321 gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggatgat
     1381 cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg
     1441 agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat
     1501 ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc
     1561 ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac
     1621 cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa
     1681 ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga
     1741 gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca gaatttcatg
     1801 ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg
     1861 gagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg
     1921 aaaggacagt aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc
     1981 tatttcgagg atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg
     2041 catgtggggt agtcagccag tggttgccag gacaaggcag ggtaatacgc atcatcactg
     2101 gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg agctaagaat
     2161 tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta
     2221 taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag atgaatacgc
     2281 gactggatcc atagactttc ttcttcactc aaccagttat taagtacgcc cattcgactt
     2341 aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca gcattcggct
     2401 gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc aagatagtca
     2461 cgccattcac atggcgaata acgaaaccag cggtgataaa accctgccaa ccgagcggca
     2521 tgaccgttat cgatcattcg gtgaattgtg cgcagcaggt catcacggtc atcaattccc
     2581 catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga tgaatgcgaa
     2641 ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattt
//
LOCUS       DAZOZD010000387         2690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOZD010000387 DAZOZD010000000
VERSION     DAZOZD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.392"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            44..769
                     /locus_tag="ACHUPB_004413"
     CDS             44..769
                     /locus_tag="ACHUPB_004413"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR0761034.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHUPB_004414"
     CDS             complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHUPB_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR0761035.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="ACHUPB_004415"
     CDS             1604..1798
                     /locus_tag="ACHUPB_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR0761036.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            2296..2457
                     /locus_tag="ACHUPB_004416"
     CDS             2296..2457
                     /locus_tag="ACHUPB_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0761037.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAZOZD010000388         2644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOZD010000388 DAZOZD010000000
VERSION     DAZOZD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.393"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            12..107
                     /locus_tag="ACHUPB_004417"
                     /pseudo
     CDS             12..107
                     /locus_tag="ACHUPB_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="ACHUPB_004418"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="ACHUPB_004418"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0761038.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="ACHUPB_004419"
     CDS             complement(1439..2392)
                     /locus_tag="ACHUPB_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0761039.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAZOZD010000389         2633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOZD010000389 DAZOZD010000000
VERSION     DAZOZD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.394"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            3..1907
                     /locus_tag="ACHUPB_004420"
     CDS             3..1907
                     /locus_tag="ACHUPB_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFR0761040.1"
                     /translation="MELPRIVKRGIIICIDVVMVIFSFWLSYWLRLDEQTAFLSAPMW
                     FAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILASTLSLVVISYSLSIMLP
                     RTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAGESGRQLLPALMQAKEY
                     FPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILLAMPSVSKSQRQKVITR
                     LEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVAPDAKLMAENITGKAVM
                     VTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELSALCDKEVLNVPVIPLL
                     GSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYCAESAIES
                     GVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTRFCMVRFGNVLGSSGSV
                     VPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMGHGGDVFVLDMGDPVKI
                     YDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEELLIGDSVQGTSHPRIMT
                     ANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFNPTDDICDLVWQQKKSL
                     LSQASNVIRL"
     gene            2026..2527
                     /gene="aqpZ"
                     /locus_tag="ACHUPB_004421"
                     /pseudo
     CDS             2026..2527
                     /gene="aqpZ"
                     /locus_tag="ACHUPB_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
BASE COUNT          605 a          552 c          671 g          805 t
ORIGIN      
        1 tattggagct gccaagaatt gttaagagag gtatcatcat ctgcattgat gtagttatgg
       61 tgatattctc attttggttg tcttattggt tgaggcttga tgagcaaacg gcttttctta
      121 gtgcaccgat gtggtttgct gcagctattc ttaccatatt taccgtgttt atatttatca
      181 ggattgggct ttatcgggca gtcttacggt atgttagtgc aaagataatg ttgctaatac
      241 cagttggtat tctggcctca acgttatctc ttgtcgttat atcatattcg ctatccataa
      301 tgttgccgcg cactgttgtc ggaatttatt ttttggtttt acttttactg acatcaggct
      361 ctagattgct ttttagaatg atacttaact atggagttaa gggtagtgcg cctgttttga
      421 tttatggcgc tggtgaatct ggccgacaat tattgccagc attaatgcag gcaaaagaat
      481 attttcctgt ggcatttgtg gatgataatc ctcgcttgca taaggctgtc attcatggtg
      541 taacagttta tccctcggat aaactgagtt accttgtaga tcgctatggt ataaagaaaa
      601 ttcttttggc gatgccgagc gtcagtaagt cacaaaggca gaaagtgatt actcgtttag
      661 agcatctacc gtgtgaagtt ctctctattc cgggtatggt cgatttagtc gaaggtcgag
      721 cacaaatcag taatctaaaa aaagtatcga ttgatgactt actaggtcgt gatccggttg
      781 ctcctgatgc caaattgatg gccgaaaaca ttactggcaa agccgttatg gtcactgggg
      841 cgggaggctc gatcggctct gagctttgtc gtcaaattgt tcgatataag ccggccaaat
      901 tggttctatt tgaactgtct gaatatgccc tctacgctat tgagaaagag ctctcggcgc
      961 tgtgtgacaa agaagttttg aatgttccag tgatccctct gttgggctcg gtgcagcgtc
     1021 agaatcgctt acagatggtg atgaagtcct ttggtattca aacggtttat catgcggccg
     1081 cttataaaca tgtgcctctg gttgagcata atgtggtgga aggggtacgt aataacgtgt
     1141 ttggtacctt gtactgcgct gagtcagcga tcgaaagtgg cgttgaaact tttgtgttga
     1201 tttccaccga taaagcggtg cgcccgacca acactatggg gacaactaag cgtctggccg
     1261 aattggtatt gcaggctttg tctgcacggc aaagccaaac tcgcttttgt atggtgcgat
     1321 ttggtaatgt actcggttct tcgggctctg tcgtgccgtt gtttgaaaaa cagattgccc
     1381 aaggtgggcc agttaccttg actcatcgtg acattattcg ctatttcatg acaattccgg
     1441 aagcatcaca gttggtgatt caagcggggg cgatggggca tggcggcgat gtctttgtct
     1501 tagacatggg cgatccggtc aagatttatg acttagccaa acgcatgatc cggttaagtg
     1561 gcttgagtgt acgggatgat aaaaatccag atggcgatat tgccattgaa gttacgggat
     1621 tacgtccagg tgagaaactg tatgaagaat tactgattgg tgattcagtt caaggtacct
     1681 ctcatccacg aattatgacg gccaacgaag tgatgctacc gtggcaggat ctatcgctct
     1741 tacttaaaga gctggatcaa gcttgtcatg actttgatca tgagcgaatt cgcagtttgt
     1801 tgttacaagc accagcggca ttcaatccaa ctgatgatat ttgcgatcta gtttggcagc
     1861 agaaaaaatc gctgttatca caagcgagca atgtcattcg cctgtgattg cttaggttta
     1921 accttccaca ccaattcttc acctctctta caaatccccg ctaggcggta catcgtgacc
     1981 gcctttaccc tgatgcctgc tctttaacaa acaggacatc agtgtatgtt taaacctttt
     2041 agcgccgaat ttttcggcac tttctggctg gttctgggtg gctgtggtag cgccttgatc
     2101 tctgctgctt tcccacagtt aggtataggc tttttgggcg tggcgttggc gtttggtctg
     2161 acagtagtca ccatggctta tgcggtcggg cacatctctg gtgcgcattt taaccccgcg
     2221 gtgaccttgg gtctgtgggc cggtggacgc ttcccagcag cgcgcgtgtt accttacatt
     2281 atcgctcagg ttatcggcgg tattgccgct gcggcagtgc tgtatggtat cgccagcggt
     2341 aaggctgggt ttgatgcgac aaccagcggt tttgcggcta atggttatgg cctccattca
     2401 cctggcggct atgcgttaag cgcctgtatg ctgagcgagt ttgtcctcag tgcgtttttt
     2461 gtccggagcg acagaaaaac gcgctcctgc gggctttgcg ccactggcga ttggtctggt
     2521 aatcaccccg taaattaacc agcgtcaaaa gtagaatttt ctcgtaccat aaacgcagga
     2581 gattctttat gaaaacatca aaatttaccg acagccaaat catggcgatc ctc
//
LOCUS       DAZOZD010000390         2625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOZD010000390 DAZOZD010000000
VERSION     DAZOZD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.395"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            109..1722
                     /gene="mppA"
                     /locus_tag="ACHUPB_004422"
     CDS             109..1722
                     /gene="mppA"
                     /locus_tag="ACHUPB_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0761041.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1773..>2625)
                     /gene="ynaI"
                     /locus_tag="ACHUPB_004423"
     CDS             complement(1773..>2625)
                     /gene="ynaI"
                     /locus_tag="ACHUPB_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0761042.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT          753 a          591 c          630 g          651 t
ORIGIN      
        1 aaacatttta tgctccctgt gtcacttttt tgatggaagt tgtttgcatt tctttaaggc
       61 gaaacaaata attacgcatc aattttaatg tcggttagag ggaaacttat gaagcactct
      121 gtttcagtca cgtgttgtgc gctgttggtc agcagcattt ctctttcgta tgctgcagaa
      181 gttccgagcg gcacagtact ggcagagaag caggagctgg tgcgccacat taaagatgag
      241 cctgcgtcgc tggatcccgc taaagccgtg ggcctgccag agattcaggt cattcgcgat
      301 ctgtttgaag gactggtgaa tcagaacgag aaaggggaga ttgtccccgg cgttgcgact
      361 cagtggaaaa gtaatgacaa ccgtatctgg acttttaccc tgcgcgataa cgcaaaatgg
      421 gcggatggca caccggtaac ggcgcaagat tttgtctaca gctggcagcg tctggtggac
      481 ccgaaaacgc tatcgccatt tgcatggttt gccgcgctgg cgggaatcaa caacgcacag
      541 gcgattattg atggtaaagc tacgccagac cagcttggcg tcaccgcagt tgatgcccat
      601 actttgaaaa ttcagcttga taaaccgttg ccgtggtttg tgaatttaac cgctaacttt
      661 gccttcttcc cggtgcaaaa agccaacgta gaaagcggta aagagtggac gaaacccgga
      721 aatctgatcg gcaatggcgc ttatgttctt aaagatcgcg tagtcaatga aaaactggtc
      781 gtggtaccga atacccatta ttgggataac gccaaaacgg tactgcaaaa agtgaccttc
      841 ctgccaatta atcaggaatc cgcagccact aagcgttacc tcgcggggga tattgatatc
      901 accgaatcct tcccgaaaaa tatgtatcag aagctgttga aggatattcc ggggcaggtt
      961 tatacgccgc cgcagctcgg gacctattat tatgcgttta acacgcaaaa agggccgacg
     1021 gcagatcagc gcgttcgtct ggcattaagt atgacgatag atcgccgcct gatgaccgaa
     1081 aaagtattag ggacgggcga aaagccagcg tggcatttta caccagatgt taccgccaga
     1141 tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg aagaactgaa tgcgcaggca
     1201 aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac cgctgaagct gacgcttttg
     1261 tataacactt cagaaaacca tcaaaaaatt gcgattgctg tagcatcgat gtggaaaaag
     1321 aaccttggcg tagatgttaa attgcaaaat caggaatgga aaacctatat cgatagccgt
     1381 aacaccggca attttgatgt tatccgcgcc tcatgggtgg gggattataa tgaaccctcc
     1441 actttcctga cattattaac gtcaacgcat tcaggaaata tttcacgctt taacaatccg
     1501 gcatacgaca aagttctggc ccaggcatcg acggaaaata ccgttaaagc gcgtaatgcc
     1561 gattacaacg cggcagaaaa aatcctcatg gagcaagcac cgattgcacc aatttatcaa
     1621 tataccaatg gacgattaat caagccgtgg ctgaaaggtt atcccattaa taacccggaa
     1681 gatgtggcgt acagccggac tatgtatatt gtgaagcatt gatgtgatgg gaactggcgt
     1741 tacccttgtg gataacgcca gtgatgtctg atttagcgcc cctgttccgg cggtgtaatg
     1801 ttatccatat acagcgtctg gctggggaag gcaaagtccg cgccgtgtga ctgcacaata
     1861 tcgataatct tcaaataaac gtcttgctgt gcagcaagcc attcagccca taccgtggtt
     1921 ttggtaaagc aataaaccat aatattcaat gacgagtcgg caaactggtt gaaataaacc
     1981 agtaaggttt gtcgctggtc gatggccggg tgatttttca gcatctcacg tacggcttcg
     2041 acaataacgc ccacttttgc cgcatcctca taacgtaaac caatggtcgt ggtaatgcgg
     2101 cggttggtca ttcgtcctgg gttttctacg ctgatcgacg aaaacagcga gttcggtacg
     2161 tacaatggac gattatcaaa ggtcgtaatt ttggtaattc gccagccaat ttccgctact
     2221 gtaccttcga tatttctgtc cggtgaacgg atccagtcgc caatactgaa aggacggtcg
     2281 aaatagagca tgatcccgga aaagaaatta ctcagaatat ctttaccggc cataccgaca
     2341 gccagaccac caataccacc aaaggtcagc aagccagaaa ggctcatgcc gaaatgttcg
     2401 ccataaagca gaacaagcac cacaataatg gtgattttga taatgcgcga cataatccgc
     2461 gcactggtga tatcgcgacc ttttttaatc tgctgttttt caaactgatt aatcagcaaa
     2521 aatagcttaa tcgtcagaat aaccgcaatc agggacgtac agataaaatc gataacgtct
     2581 ggggtgataa atttgagttt atagttttct ataacataat taata
//
LOCUS       DAZOZD010000391         2608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOZD010000391 DAZOZD010000000
VERSION     DAZOZD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.396"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACHUPB_004424"
     CDS             complement(122..874)
                     /gene="tcyN"
                     /locus_tag="ACHUPB_004424"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0761043.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACHUPB_004425"
     CDS             complement(871..1539)
                     /gene="tcyL"
                     /locus_tag="ACHUPB_004425"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0761044.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACHUPB_004426"
     CDS             complement(1554..2540)
                     /gene="dcyD"
                     /locus_tag="ACHUPB_004426"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0761045.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT          622 a          733 c          648 g          605 t
ORIGIN      
        1 ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt
       61 tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt
      121 attattgcag tagaaacttc tcgaggaact ggcgggtgcg aggctgctgg ggatcagcaa
      181 ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaaa atcgcccggt
      241 ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg cgcttttcct
      301 gcgccagctg gcggattgtg ttcaggactt cgcccaccag ctctggatcc agcgctgacg
      361 ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca atcgctacac
      421 gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa
      481 ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga
      541 tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa ttaaagttct
      601 ggaagacaaa cccgacgtgc tgacgcaact ggcggatcag cgatttttgc tgacttaatg
      661 aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca
      721 gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg ataattgcca
      781 ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga ccgtgaaatt
      841 ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag
      901 ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc agtagatgag
      961 cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca actgcgcctg
     1021 acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatccttta ccaggctgat
     1081 aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg
     1141 catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac ctttatcaat
     1201 agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg gcagtgttca gcgacagacc
     1261 aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc cgtaatagat
     1321 cataaacagt tgtgcgatga gcggtgtacc acgaaaaata gagatataaa aacgcgccag
     1381 ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc cgagcagtaa
     1441 gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt tcagcaggaa
     1501 cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg
     1561 tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag aatcggccct
     1621 tcatctttga agcgtttctg actgatacag ccaatcagcc ccgccatcgc ttttccggta
     1681 tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct
     1741 tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtat aatttccgct
     1801 gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaac
     1861 tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt
     1921 tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac taccaccgac
     1981 gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct
     2041 agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct
     2101 tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc ttcgcacatt
     2161 tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa atagttttct
     2221 gcggttgtgc caataggatt ttccagcagc gccacacagt gcagaccgag tttcgccgca
     2281 actgcggcag tctgccgcac atggttagac tggatcaccc cggcagtaat cagcgtatcg
     2341 gcaccttcgc gcagagcatc tgccgcgaga aattctagct tacgtaattt attgccgccc
     2401 attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag ataatcagaa
     2461 aagcgcggca gatattcgag cggcgttggc gcgccgataa actccaaccg tggaaaacgg
     2521 gttaaattat gcagtggcat aacagcctcc gatgtatgtt gttgtgattt tcttattatg
     2581 cacgctgaaa acgcgtaaat aaaaaagg
//
LOCUS       DAZOZD010000392         2607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZOZD010000392 DAZOZD010000000
VERSION     DAZOZD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.397"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..471)
                     /locus_tag="ACHUPB_004427"
     CDS             complement(<1..471)
                     /locus_tag="ACHUPB_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0761046.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="ACHUPB_004428"
     CDS             complement(471..1391)
                     /locus_tag="ACHUPB_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0761047.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHUPB_004429"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHUPB_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0761048.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          661 a          606 c          591 g          749 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcat
//
LOCUS       DAZOZD010000393         2600 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZOZD010000393 DAZOZD010000000
VERSION     DAZOZD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.398"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..493
                     /gene="virA"
                     /locus_tag="ACHUPB_004430"
     CDS             <1..493
                     /gene="virA"
                     /locus_tag="ACHUPB_004430"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR0761049.1"
                     /translation="QVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTSAFWQS
                     LHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAISASGG
                     RAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVSFAIIS
                     PDV"
     gene            672..1359
                     /locus_tag="ACHUPB_004431"
                     /pseudo
     CDS             672..1359
                     /locus_tag="ACHUPB_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    952..1068
                     /locus_tag="ACHUPB_004431"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1361..1727
                     /locus_tag="ACHUPB_004432"
                     /pseudo
     CDS             1361..1727
                     /locus_tag="ACHUPB_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1730..2128
                     /locus_tag="ACHUPB_004433"
                     /pseudo
     CDS             1730..2128
                     /locus_tag="ACHUPB_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          701 a          631 c          684 g          584 t
ORIGIN      
        1 ccaagtaaat aaaataatca attcaaactt cccccaacaa gacctatgtt ttcaaacaga
       61 aaaacttttg tttacttcac tctttcaaga ccctgcattt atatctgcac taacatctgc
      121 attctggcaa tctcttcaca tcacgtcttc ctctgtggaa cacatatatg cccaaattat
      181 gtccgaaaac atagaaaaca ggcttaattt catgcctgaa caacgagtta ttaacaattg
      241 tggacatatt ataaaaatca acgcagttgt ccctaaaaac gacactgcaa tctctgcctc
      301 tggggggagg gcttacgaag ttagctcatc aatattacca tcgcacataa catgtaatgg
      361 tgttggtatt aataaaatag aaacaagcta tctcgttcat gctggaacac ttccaagcag
      421 tgaagggctt cgcaatgcta tccctccaga aagtcgtcaa gtatcatttg ccatcatatc
      481 tcctgatgtt taagccaata ttatattggc tgccagactg taaaaaaacg ttcttcagaa
      541 cagctctata aactattaaa ccaaagctaa aaggatgggg accaatagaa tctcagccca
      601 tcctttttat atcattatga accgccccgg aaatcctgga gactaaactc cctgagaaag
      661 aggtaaacag gatgactaaa aatacacgtt tttcccctga agtccgtcaa cgggcagttc
      721 gtatggttct ggaaagtcag ggcgaatatg actcacaatg ggcagcgatt tgttccattg
      781 ccccaaagat tggctgtata ctggagactc tgcgtgtctg ggtacgccag catgagcggg
      841 ataccggggg cggtgaggtg ggctcaccac cgctgaacgt cagcgtctga aagagccgga
      901 acgtgaaaat cgtgaactgc gccgcagtaa caatatcctt cgcctggctt ccgcttattt
      961 tgcaaaggcg gagttcgacc gcctctggaa aaaataatgc cactgctgga taagctgcgt
     1021 gagcagtacg gggtcggatc ggtatgcagt gaactgcata ttgccccgtc aacgtattac
     1081 cactgtcagc aacagcgaca tcatcatgat aaacgcagtg cccgtgcgca gcgcgatgac
     1141 tggctgaaga aagagatact gcgcgtatac gatgagaatc atcaggtata cgctgtgcgt
     1201 aaagtctggc atcagttgtt acgggaaggt atcagagtgg ccagatgcac tgtggcacgt
     1261 ctcatggcgg ttatggggct tgccggtgtt ctccggggta aaaaggtcca cactaccgtc
     1321 agccggaaag ccgttgccgc aggcgaccgc gtaaaccgtc atcaaggcaa tatgccgcgt
     1381 actccgggtg gcccgcagcg gctggtatac gtggtgtcag cggcggacaa ggataaacac
     1441 acgtcagcag ttccgtcagc actgcgacag cgttgtccgc aaggctttta cccagtccag
     1501 cgttacggtg ccccacgcct gacggatgaa ctgtgtgctc tggttaccac tttaacgtaa
     1561 aaaccgtggc agccagtctg cgccgtcagg aactgagcgc aaaagcctcc cagaagttca
     1621 gcccgatcag ttaccgtgca cacggcctgc ctgtatcaga aaatctgctg acgcaggatt
     1681 tttacgccag tggcccgaac cagaagtggg cgggagacat cacgtactat tacagctcac
     1741 cgactgcggg gaaacacggg gcgccgggtt attgaaggtg acctgtccag ctacttcgac
     1801 acagtacatc accggctgct gatgaaagcc gtctgccgca ggatcagtga tgcacgtttt
     1861 atgagactgc tgtggaaaac gccgtgttga tgttggtctc ttccgggcgg tcagtgaaag
     1921 tatgccacag ggcggtgtga tctcgccgtt gctgtcgaac atcatactga atgagttcga
     1981 ccagtatctg aataaacgct acctgagtgg aaaagccaga aaggatcgct ggtactggaa
     2041 tcacagtatc cagcgaggcc gaagtactgc tgtcaaagaa aactggcaat ggaaacctgc
     2101 cgttgcttac tgttgctatg ccgatgactg tgtgccacga aggcgcgtcc tggggacgtg
     2161 agtccctgtg cagccatgcg gcacaagaga tgagggaagg cccctcggtg tcggcgtgca
     2221 ggcgcaggca ccaaaccatc cactgctgct gcatttaaga gatgtggtgg tgagcgagat
     2281 gggaaaaggt cggacgtgag ccgatcaggt atggttcgaa gagacgaacg aaagtgaacc
     2341 gctgatgatg tgtcgaaagc atatagatga tgtcaaaaca atggtggagt cgttcctgtg
     2401 ggatatgttt actgcctgcg cggcatccgg cataaaagcg gcgtgaagtc gattcaggct
     2461 cttgtgggga acgtggacct gtcaccccga tgtgaaggga gaagcgcaag tggaagcccc
     2521 atgagcgcca gagtaccaat gcggggtaca gggacggagc accaagcgat tgcgcagtaa
     2581 acatgcttga caacttaatt
//
LOCUS       DAZOZD010000394         2596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOZD010000394 DAZOZD010000000
VERSION     DAZOZD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.399"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            111..443
                     /locus_tag="ACHUPB_004434"
     CDS             111..443
                     /locus_tag="ACHUPB_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0761050.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUPB_004435"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="ACHUPB_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0761051.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..>2596)
                     /locus_tag="ACHUPB_004436"
     CDS             complement(2281..>2596)
                     /locus_tag="ACHUPB_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761052.1"
                     /translation="KKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDNQ
                     YYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKC
                     GE"
BASE COUNT          680 a          672 c          619 g          625 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttc
//
LOCUS       DAZOZD010000395         2591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOZD010000395 DAZOZD010000000
VERSION     DAZOZD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.400"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHUPB_004437"
     CDS             <1..189
                     /locus_tag="ACHUPB_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761053.1"
                     /translation="CGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQ
                     IERNAKARIRRATKSRTE"
     gene            282..500
                     /locus_tag="ACHUPB_004438"
     CDS             282..500
                     /locus_tag="ACHUPB_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0761054.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            502..867
                     /locus_tag="ACHUPB_004439"
     CDS             502..867
                     /locus_tag="ACHUPB_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0761055.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            864..1529
                     /locus_tag="ACHUPB_004440"
     CDS             864..1529
                     /locus_tag="ACHUPB_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761056.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1529..1894
                     /locus_tag="ACHUPB_004441"
     CDS             1529..1894
                     /locus_tag="ACHUPB_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0761057.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2349..2504
                     /locus_tag="ACHUPB_004442"
     CDS             2349..2504
                     /locus_tag="ACHUPB_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0761058.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          714 a          545 c          701 g          631 t
ORIGIN      
        1 tgcggtgcgg ttgtggagcc gtttgactgg atgctctctg tggcaaaaag agaaaccaga
       61 ctggcagatg atgtaaggct attgcgccag gaggaacagg aaaggcggag aaatatagaa
      121 aagctgatac agattgagcg taacgcgaaa gcgcggatac gcagggcgac aaaatccaga
      181 actgaataat taaatttagc actgttaaaa atttaatcct taaccggagg gatttctgca
      241 ccctcaaatc atcaggagac cgcccgaaag ggcggtaatg aatggttaca ttatttagaa
      301 aaaaatatcc gcgaaagagt agaacaacag aattcctgtt tctcattctg tttatcgtgt
      361 tgatgacacc gatatccccg ctaatttttg tctggataat cggaaaaata attgagccag
      421 ttattgaatt gtataacgac gtggtatggg cgtcattcaa cacactgcac aataaaatta
      481 atccgtataa ggaaagctga tatggcactg acgaaaaaac aacgtgcaga actgcgcatg
      541 aagttcggtg gtcgctgcgc ttattgcggc tgcgaacttg gcgaaaagtg gcatgcagac
      601 catgtaaaac cggtcattcg ttttgatgga aatatgcttc accaggaacg tgacgatata
      661 tccaacatgg taccagcatg ccacccatgc aatctgcaca agcattgcag tagcctggaa
      721 gattatcgac gaattatcag tgatggtcgt cgtgaattcc ttgcgtccgg gaaaggcaaa
      781 gcgctggttc gtatgggatt ggttgaaatg aaatctgacc cggttgtgtt ctggtttgaa
      841 aaatatcaag aaggggctac ggcatgacca ctattaccaa agagcgactg caatggctgg
      901 ctaacatttc tggccgcgat gatattgacg atatagacgg cggtgaaatt cgtgaactgg
      961 cgcttatcgc tctggcatca ctggaagcag agcctgtagc ggaatgcatt gttgaagatg
     1021 ggggcatgtg tattgacggg ttcggtgagt atgtgggtca ctcgctgcct gatggaatgc
     1081 atgagcttta tgctgccccg cgaatgcgac aacctgatgg atggaaagcc gtagctgtag
     1141 cgtggaaggt gacgtttact caagttgacc aggaatctaa tacgttcact gctatatatt
     1201 ttgacaaagc ggaagttgag tgttgggtac gactgcataa agcatgtgat tttcgggcag
     1261 atataacacc gctttacgca gcgccggcag tgccggttgc agtaaacgac gacatggctt
     1321 acgcattcca tcacgcactg tcagattcat cgcttggctc tgatgaaatc gaagaaatta
     1381 aaaccggttt gcgtgctgcc tttgccaacg tcactatcca accagagccg atagtgccgg
     1441 atgaaatcgg gccaaacgat agtaatacgt ttgattatgt tgatggctgg aacgcctgcc
     1501 gcgctgccat gcttaaggga gataaataat gattaatcga accaaactgg agcacattct
     1561 cgaatatgcc aggcagcaga aatgtattgg gcaactttgt aaaattccac caggagatat
     1621 ggttgaaatc gtggaaatgg ccatgcgtaa ggctggcaac tctccggtag ctccggctgg
     1681 ctggataagc tgtagtgatg cagttcctgc tgaatattgc gatgtgattc ttctcgatga
     1741 tctcgggaat gtattccccg gttcctggga taaggttttt tgccccactc gtggcgggaa
     1801 taagatggct tttgtggaca aagacggcgt tgaagtagag agctcaactc actggatgcc
     1861 gctaccggaa ccaccgcagg aggtgaatca atgacctggc ctgaggcatt caccacggta
     1921 ggaattgcga tggcggtggc gctggtggtg tattcgattt gccgttgggg ttaacaaaca
     1981 aaaaccccgg attgatggtc cggggttttt gaaggaaaca aaacagaaac agcaattgcc
     2041 gttacctgtt gttaccatgg caagtaaacg tatctcaggc gagcgcattg cgccgttctg
     2101 acgcagataa actagcctgg atagatggtg ctggcaataa aaaatagcgt tttcttatcg
     2161 gtgtcggtaa gattgctgcg ggtgcttgag gctgtctgcc tcgggtatgt cactgtaagg
     2221 cagacagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa cattaatctg
     2281 aggcccaatc tatgtctcac aaatgtaggt tagcctctta cgtgccgaaa ggcaaggaga
     2341 agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc tgtttaaccg
     2401 tcattgtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc cgaatcggcc
     2461 agacggaggt cgctgttttc gtagactacg aatctagaga gtaagagtga ccaggcggga
     2521 gagtaatctc ccgccacctt tgatgtgtca ggcatcctca acgcacccgc acttaacccg
     2581 tttcggcggt t
//
LOCUS       DAZOZD010000396         2564 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOZD010000396 DAZOZD010000000
VERSION     DAZOZD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.401"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            4..210
                     /locus_tag="ACHUPB_004443"
     CDS             4..210
                     /locus_tag="ACHUPB_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761059.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            309..2489
                     /locus_tag="ACHUPB_004444"
     CDS             309..2489
                     /locus_tag="ACHUPB_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0761060.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
BASE COUNT          817 a          441 c          604 g          702 t
ORIGIN      
        1 tgaatgattg atttaacaga acgctatgag cttattaaag caattagtga acgattgaaa
       61 gaacaaccca acaaaccaaa acgaatcaaa tcattcacct cctctaagca aaagaataga
      121 atcaggaaaa tagctaagtg gtgtcttgat aaattaccaa gtgattgtca gattgttggt
      181 gaagatggta attattatct ggttaggtaa caagattggt tgggtgaaaa tctacgggag
      241 agattatcga gtgatatcgg tagtctttcc ctttttcgta tatgaacact taaagaggtg
      301 aaacatgaat gagcaaattc acagttgata gttttatcgt tgaacttagt ttcagtgaaa
      361 acgttattaa gggcttgcaa agagttgaaa aggcagcttt gcagtcagca caaagaattg
      421 aacgcaattt gaatagagcg tttaaggtcg ataccaagca acttgacagc aatctgacaa
      481 gttctttagg gcagcttgaa aggaagttta ataagacctt tgacaagatt gagcagagag
      541 caagaagcac aaaagcattt cagtttgcca ccagatttaa tgacactgtt aatcctccaa
      601 aacaatcaag gcaaccacga attagcggaa acagagcaat cacagcagcg catagcgtga
      661 atatgtctaa gcttggtgat attaatccgc tgatggctaa aatgtttaaa gcacagtatt
      721 acagcttaag cggtaaggct ggtaatattg gaagtgaaaa gtttaacgct gaacttgcaa
      781 aattaaatca atctcttcgt gaaacgttga ataaactcag gtcacagaca aagaccgcca
      841 gcattaacaa tacaagcgac gcatttaata atcttgcaag caatgcgatt aaattatctg
      901 gtgcatttta ctctgtgatg ggtgctctta acgcttacaa ggccattatg aatgcaggtc
      961 ttaaacgaga ttccgcacag agagccgcta agtttgtact tggtgataaa gcatctgaag
     1021 ctgaggtatt tattcgaaat ttagcagaca agacaggtct taatatctct gaagggctat
     1081 caagctacgc taagtttgca gcaggtgctc agggttctat gtcacaggaa cagacacagg
     1141 agctatttgg taatgcaacg gctatgagtc gcttaatggg gctttctaac gatgagctaa
     1201 acggcatttt gagagcattt gagcagatgg cgagcaaggg caagattcag gctgaggaat
     1261 tacgtgggca gttaggtgac cgtatggctg gtgcatttaa gctattcgct gaagcattgg
     1321 gtatgactgc tacagagctt gatgcagcca tgaaaaacgg gaaggtactt tccgctgata
     1381 ctctccctaa agtcgctaaa cagatgggct tgatgattga taaagcgggt ggctgggctg
     1441 aagtcactaa gagcactcag acagctttag gtaagttagc aaacaattgg gatgatacga
     1501 tggtgaaaat cttctcaggt tcacaggatg agctaaacgg cttcttatcc agcctgagta
     1561 acctgcttag tgaaatgggg atgagttctt ctatcgctgg tgatgctatc ggtggactta
     1621 tcgacatgct taaagctggt gtagatgata ttcgcgtatt caataacacc attgaaggct
     1681 ggattttaca gactaagaag ttctattaca gccttgatga cactaaacga aaactacttg
     1741 atgaagtcgg tgatggtttt atcaacttcg tcaaaggtct tgctatagcg ttatctgcaa
     1801 agacgctatt cagtgctact acaggggtta tgaacctgac aagagcaatc acaacgttag
     1861 gtacaagagc taatcaggtg gctggtcagg tagctactgg taaaggtggt ggaaaactga
     1921 aaggtgtagc tggtggtgta ggtagtgctc ttgcaattgg ttatgctgat gacggttatg
     1981 aaacagcgtt agcattagct tcaatgattc cacaaatcag aggcgtcact cttggtttat
     2041 atgcattgaa gaaagcactg gattttatga atcaggaagt agtcaaaaac gctaatatgc
     2101 atccgtctgg ggtaggtgtg ggtagtgact ttaatcccgt gtattctggt gaccctaata
     2161 aaccgaatat gattaacgaa ggatggagta gaatttttag ttcaatgggt gaattgttta
     2221 acaatgccac aatgaatatt gcaagattca atcatccaga gttaaacaac attaagcaag
     2281 attcagcatt aacgagtgca gatattcagg gtctgaggga tgaaattagt gctttgagta
     2341 agcgtattca ggagccagtg aaagtttcac ttggtggtca agttgtcgtt cgaccagatg
     2401 aaccatcttt ctttactttc aatcaatcag ctaatgacca gttcacgcag gatatgctct
     2461 taagttcaag ctatccagag gaagaatgat ttaacggtgt tctacgtgaa taaagcaaag
     2521 ttaataccca caccaaaaat tatttttcac gtagctcacg ctta
//
LOCUS       DAZOZD010000397         2562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOZD010000397 DAZOZD010000000
VERSION     DAZOZD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.402"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            431..574
                     /locus_tag="ACHUPB_004445"
     CDS             431..574
                     /locus_tag="ACHUPB_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0761061.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            587..796
                     /gene="cspE"
                     /locus_tag="ACHUPB_004446"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             587..796
                     /gene="cspE"
                     /locus_tag="ACHUPB_004446"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0761062.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            962..1771
                     /gene="rlmA"
                     /locus_tag="ACHUPB_004447"
     CDS             962..1771
                     /gene="rlmA"
                     /locus_tag="ACHUPB_004447"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0761063.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1768..2334)
                     /gene="mntP"
                     /locus_tag="ACHUPB_004448"
     CDS             complement(1768..2334)
                     /gene="mntP"
                     /locus_tag="ACHUPB_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0761064.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          672 a          599 c          616 g          675 t
ORIGIN      
        1 cttgattttt ttgctcattc atcaattttt cttattttaa attttcaatc cctttgggga
       61 ttgacttctc tttagggtaa ttgatagccg ttaaccgact gttttatgag aaaaagtgat
      121 ataacttttt attcattgca tagcaaaaaa tgtgatattg cacgcactat gtaataactt
      181 ctcccactgg cctggaacaa ctgaacttat tgaactatgt tagaaaatac gccagtttaa
      241 gtatctgcct gaactggcaa ggttaagcac aatgatatat cggcgcgtat tccgttgcat
      301 aagtgtgcaa aaaaagtgga agacgtatcg agatttgtgc gtctgatcga gacatgttta
      361 aaaatggctt gccataatta acgttgtatg tgataacaga tttcgggtta aacgaggtac
      421 agttctgttt atgtgtggca ttttcagtaa agaagtcctg agtaaacacg ttgacgttga
      481 ataccgcttc tctgccgagc cttatattgg tgcctcatgc agtaatgtgt cagttttatc
      541 tatgttatgc ctgcgggcga agaaaacaat ctaaggaatt tttcaaatgg caaagattaa
      601 aggtcaggtt aagtggttca acgagtctaa aggttttggc ttcattactc cggctgatgg
      661 cagcaaagac gtgttcgtac acttctccgc tatccagggt aatggcttca aaactctggc
      721 tgaaggtcag aacgttgagt tcgaaattca ggacggccag aaaggtccgg cagctgttaa
      781 cgtaacagct atctgatcga atccactgat ctgaagtgtg aatacgcttc aatctcgcta
      841 taaagcctcg tcgaatgcga ggctttttac tatgctttat cttcgctcct ggcgttcgga
      901 tatttgcccg ccgcgtgatt cgcgttacac ttgcggcctt tagtatcctg ccggagttgt
      961 catgtctttt tcctgtccac tttgccatca gcctctttcg cgtgaaaaaa acagctatat
     1021 ctgtccccag cgacatcagt ttgatatggc gaaagaaggg tatgtcaatc tgctgtccgt
     1081 tcagcataaa cggtctcgtg atccgggcga cagcgcggaa atgatgcaag cacgccgcgc
     1141 attcttagat gccggacatt atcagccgct gcgtgatgca attgtcgccc aactgaggga
     1201 acggcttgat gataaggcca cggcggtgct ggatattggc tgtggtgaag ggtattacac
     1261 acacgcattt gccgatgcgt tgcccgaaat caccacgttt ggtctggatg tttcgaaggt
     1321 agcgataaaa gcggcggcga aacgctatcc gcaggtcact ttttgtgtcg cttccagcca
     1381 ccgtttgccg ttttccgata ccagtatgga cgccataata cgtatttacg cgccgtgtaa
     1441 agcagaagaa ttagcacgag tagtgaagcc cggcggctgg gtcattactg ccacgccggg
     1501 accgcgacat ttgatggagc tgaaggggct gatttacaat gaagtacatc ttcatgcacc
     1561 tcatgcagaa caactggaag gttttacatt acagcagagt gcggagttgt gttatccgat
     1621 gcgtcttcgc ggtgatgaag ccgtcgcatt attgcagatg acgccgttta cctggcgtgc
     1681 gaagccagaa gtctggcaaa cactggcagc aaaagaagtg ttcgactgcc agacggactt
     1741 taatattcac ctctggcagc gttcttatta accgtggaag tgcgtccaga ggatctggac
     1801 gccgatgccg atcagtacca gcccgccgag aatttctgct tttttcccaa taattgagcc
     1861 gataaagcga ccaaccatca tccctaatgt tgacataatc aaggttgcac aaccaatggc
     1921 caatgcggtc gcgataatgt tgacctgcag gaaagcaaga ccaacaccca cagccatggc
     1981 atccaggctg gtggcaatcg cggtggttac cagtagccag aaaccgtgtc gacggcgcgg
     2041 ctcttcatct tcatcatctg cgccacgaaa accctcaata atcattcgcc cgccgaggaa
     2101 tatcagcagc acaaacgcaa tccagtggtt ccattcaagg acaaaccggc tggctaacat
     2161 gcccattccc cagccgatca gcggcgtcag ggtttcgacg gcaccaaaaa taaggccggt
     2221 tcgcaatgct tcagaaaatt tcggtttatg gagggtggca cctttaccga ttgatgcagc
     2281 aaatgcatcc atcgacatac caaacgcaag aagaacagta gcagtgatat tcatgacaat
     2341 gtcctgaccg gggtatccat aaaacacatc atcgccccca gcaaacatgc atacatgaac
     2401 aacaagccgt gatggttttc gcgttcatgc aggcatagcg attgatgtgc atatggtctc
     2461 gcctgactga aagtattcag tccgtacgtg ccacgttttg caacgagtat gttgacacgt
     2521 acatttccgg aatctggaaa tcggctactc cccaaatgag gg
//
LOCUS       DAZOZD010000398         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOZD010000398 DAZOZD010000000
VERSION     DAZOZD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.403"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUPB_004449"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUPB_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0761065.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUPB_004450"
     CDS             complement(390..1178)
                     /locus_tag="ACHUPB_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0761066.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUPB_004451"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUPB_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0761067.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUPB_004452"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUPB_004452"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0761068.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUPB_004453"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUPB_004453"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0761069.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZOZD010000399         2490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOZD010000399 DAZOZD010000000
VERSION     DAZOZD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.404"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..470
                     /gene="eco"
                     /locus_tag="ACHUPB_004454"
     CDS             <1..470
                     /gene="eco"
                     /locus_tag="ACHUPB_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0761070.1"
                     /translation="VLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQE
                     DESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPD
                     GKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR"
     gene            complement(779..2425)
                     /gene="mqo"
                     /locus_tag="ACHUPB_004455"
     CDS             complement(779..2425)
                     /gene="mqo"
                     /locus_tag="ACHUPB_004455"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR0761071.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          564 a          708 c          634 g          584 t
ORIGIN      
        1 cagtattgtt tgccgctttc gccaccactt ccgcctgggc ggcagaaagc gtccagccac
       61 tggaaaaaat cgcgccttat ccacaagctg aaaaagggat gaagcgtcag gtgattcagt
      121 taaccccgca agaagatgaa tctaccctga aagtagaact gttaaccggt cagacgctgg
      181 aagtcgattg caatttgcat cgtctcggcg ggaagctgga aaacaaaacg ctggaaggct
      241 ggggctatga ttattatgtc tttgataaag ttagctcgcc ggtttccacg atgatggcct
      301 gcccggatgg caagaaagag aagaaatttg tcaccgcgta tctgggcgat gctggaatgc
      361 tgcgttacaa cagcaagctg ccgatcgtgg tgtatacgcc agacaatgta gatgtgaagt
      421 accgcgtctg gaaggcggaa gagaaaattg acaacgcggt agttcgctaa actgctgtga
      481 agtgcgtcac cccgtaggtc ggataaggcg gtcacgccgc atccgacatc taacgcccaa
      541 gccggttgcc tgatgcgacg ctggcgcgtc ttatcaggcc tacaccgctg tgaagtgctc
      601 caccccgtag gttggataag gcggttacgc cgcatccgac atctaacgcc cgagccggtt
      661 gcctgatgcg acgctgacgc gtcttatcag gcctacaccg ctgtgaagtg ctccaccccg
      721 taggtcggat aaggcggtta cgccacatcc ggcataccat gccggatgtg gcacatcatt
      781 acaacgcaat atccgccact tctttttgaa cgggttgcgg tttcaactgc ggtttcggcg
      841 tactatctgc tgcctgcggc ttgtcgtatt tcaggcccag cacttcgctg gtgtactgca
      901 actcgcgttc tgttgccgct acatcaccgt tcagcttgcg tccataagat ggaacgatcg
      961 ctttcaacgt agcctgccat tgcgggctgg aaacgcgatc gccaaatact ttttccagca
     1021 gatccaacat aatcggcgca gcggttgacg cccctggcga tgcccccagc agcgcggcaa
     1081 tggtcccttg ctggtcactg accacttcag tacccagacg cagtacacca cctttatcgg
     1141 catcacgctt gataatctgc acacgctgcc ccgcttgcca caaacgccag tcctcttttt
     1201 tcgcctgcgg atagtactct ttcaacgctt caaaacgatc ctcttcactc aacatcacct
     1261 gactcaccaa gtatttcacc agatcgaaat tatccagccc gacgtgcatc atcggcatca
     1321 cgttagaggt ggtggtggaa ctcatcagat cccacaatga accgtttttg aggaatttgg
     1381 ttgagaaggt ggcaaatggc ccaaacagca ctacgcgttt accgtccaga acgcgggtat
     1441 cgatatgcgg aaccgacatc ggtggtgcgc caacggatgc tttaccgtaa accttcgcca
     1501 gatggtgatt aaccacgtcc gggttttccg aaacaaggaa ctgtccgccc accgggaaac
     1561 cggcgtagtc tttcgcttcc ggaatccccg attcctgtaa cagcttcagc gccgcaccgc
     1621 ccgcgccgat aaagacaaat ttggcacgaa tgttctgtgc agtgccattt ttcagatcgg
     1681 caacggtaac ggtccaggtg ttatcgtcat tacgctttag ggcgcggact tcgctgctga
     1741 gttgcagcga gaagttagat ttcttctgca aggaagcaat taactggcgg gtgatctcgc
     1801 cgtagttcac atcggtacca atttccgtac gcgtggctgc cactttctgt tgcggatcgc
     1861 gcccttccat cactaacggt gcccactctt tgatctgcgc gtgatcttca gagtaacgca
     1921 taccgcgaaa cagcgagctt tgttgcaacg cggcgtaacg ggcgcgcagg aaattaacgt
     1981 tatcctcgcc ccagacaaag ctcatatgcg gaacggtatt gataaatgaa cgcggagtac
     2041 gcagcacgcc gcgctcgacc tggtgcgccc agaactggcg ggaaatctga aatgcttcgt
     2101 taatggcgac tgctttttca atactgatac tgccatcggc gttttgcggg gtgtagttca
     2161 gttccatcag tgcagaatgc ccggttccgg cgttgttcca gccgttcgaa ctctcctgcg
     2221 cgacaccctc caggcgctcc accatggtca tcgaccattc aggctccagc tcgcgtaaat
     2281 aggtccccaa cgtggcgctc ataatgccgc cgccaatcaa cagtacatca gtttcctgct
     2341 cctcggacgc tttcgctttt gccgccatcg aaacggcatt aagccccacg gccatcgaga
     2401 agagcatggc agtcactttt ttcatcttgt taatgcctta cttttagtcg ctttattgca
     2461 ggtgagattt gcgcggcatc aacggtaaca
//
LOCUS       DAZOZD010000400         2482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOZD010000400 DAZOZD010000000
VERSION     DAZOZD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.405"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(782..1333)
                     /locus_tag="ACHUPB_004456"
     CDS             complement(782..1333)
                     /locus_tag="ACHUPB_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0761072.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            2132..2428
                     /locus_tag="ACHUPB_004457"
     CDS             2132..2428
                     /locus_tag="ACHUPB_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0761073.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          705 a          527 c          478 g          772 t
ORIGIN      
        1 agacttttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
       61 tcccttcttt ctaggtggtt atctgttaag agtttttcag tttttattct taagataatt
      121 gttaagatga tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt
      181 cacagtcaga ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc
      241 tctgagttta tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg
      301 cttaggtgct gctaaggctt acttagtgga aagagagggg atatgtgacc ataggggaac
      361 ccctcctgtt aaaagactac ttagaagtct gaggttttag taaaatctgt gaatatggct
      421 tctcagacgc tctgtaacgc attctaagcg attttacccc tttattttaa ggtggttagt
      481 acatccctat aggttacccc taaaaatggg gttaaaactc gctacagagc tttctatgag
      541 attagcttct aatcgaatca actatgaggt ttggtaggat tgtgcaaaat ctgtacagta
      601 acttttcaga atctgaaaat ttttataaaa aatgactagc cctctttgaa tctcatcttc
      661 cccgaatctt caaaaatatc tgacagatgt ctgtagctgc taagtacccg tcacaccagc
      721 tatgtgaccc ccttggcaaa aaagccaaat atcagctaca tccatgtaac tattcagtaa
      781 cttatttagc aagtctgtaa taagttgctc tagagattcc taactcttcc tgaatctgca
      841 acctcgtctt accttgtgaa ataagcgtct gtacagcctc tgaagaagct tttacaggcc
      901 tccccatatg taccccttca gctttagcac gttcaatacc agcctttcta cgctctgcta
      961 tcaagcttct ttcaagctgt gccaacgttg acataatgcc taacattgct tcacccatag
     1021 cgcttgataa gtcaaaccct tctctaaggc tgataactgt cacaccttta gcctgtagcg
     1081 ctttaaccgt tgataacaca tcaattgtgt cacgtccaag acggtctaca gctaccacaa
     1141 ctacggtgtc accttctcgt gcataagcta atagtctgct gaaaccgttt cttaaagaag
     1201 ctttgacaga accactgaca gcttcatcag tgaaccattt agcaatgttg taccctgcct
     1261 cttcaatttg catttgctgg ttagtgatgg attggtcatc tgtactcacc ctgcaatatc
     1321 caagtatcgc cataatgttc cccttagaag tctcaaaagt tagtctcata agtttagctg
     1381 tctcataatc caaaaacaac cttttgagac tacttgaggg tgcattctac cgttacgttg
     1441 aaagtgtctc aaaaggaagt ccttttaaga ctggttatga aggagctatg aggttgatta
     1501 ggggttggta caagcccaac taaccgaatc aactatgaag attttaggag ggtaactgta
     1561 accatcactt tctaactttc acgtaggaaa ttgttaagtg gtttgatagt tacaccatgt
     1621 gaccaagcat ctatatatcg ttatatagcc ctgtatatag cactatatgt agtgtcgaga
     1681 gaggaacgac taacacgata cagtttaccc tgaaaagaga cttcacagat tctttatata
     1741 tttcaataag ttagggtaaa aagggactat accgagactg tatagaatct gtgtagaatt
     1801 gcatagatac ttggaaatct actattaaat tgcataggga ctcacctgat attgtcctgt
     1861 ttttaacagg tctcctagaa aaccgaatag acttcattca agggtcattg aagtcataat
     1921 catttcagta gaaaaaccaa gtctcgattt taaatgcaaa tagcaatcat attcaataag
     1981 gcaagacgat caggatcagt gaaaatgtca tttttggcgt aattgacctt actttatgca
     2041 tggtcttaac aaagacatca tctttccgtt gcagatcttc gccctttcaa ataacgtact
     2101 ttacaacttt cccgaacaag gagttgtgcc cgtgttgtat gttatttacg ctcaagacaa
     2161 agctgatagc ctcgaaaaac gcctttccgt tcgtccggca catttagcac gtttacagtt
     2221 actgcatgat gaaggtcgtt tgttgacagc tggtccaatg ccagcggtag acagtaacga
     2281 tccaggggct gcgggattta ctggctcaac cgttattgct gaatttgaat cgctggaagc
     2341 agctcaggcc tgggccgatg ctgacccgta cgtagcggca ggtgtctatg aacacgtttc
     2401 agtgaaacca tttaagaaag tgttctgaat agagattcaa tatttaaggg cacggttttt
     2461 gcaaccgtgt tcttgtttgg cc
//
LOCUS       DAZOZD010000401         2462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOZD010000401 DAZOZD010000000
VERSION     DAZOZD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.406"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..2461
                     /locus_tag="ACHUPB_004458"
     rRNA            <1..2461
                     /locus_tag="ACHUPB_004458"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          636 a          540 c          782 g          504 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
      661 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
      721 gaagctgcgg cagcgacgct tatgcgttgt tgggtagggg agcgttctgt aagcctgcga
      781 aggtgtgctg tgaggcatgc tggaggtatc agaagtgcga atgctgacat aagtaacgat
      841 aaagcgggtg aaaagcccgc tcgccggaag accaagggtt cctgtccaac gttaatcggg
      901 gcagggtgag tcgaccccta aggcgaggcc gaaaggcgta gtcgatggga aacaggttaa
      961 tattcctgta cttggtgtta ctgcgaaggg gggacggaga aggctatgtt ggccgggcga
     1021 cggttgtccc ggtttaagcg tgtaggctgg ttttccaggc aaatccggaa aatcaaggct
     1081 gaggcgtgat gacgaggcac tacggtgctg aagcaacaaa tgccctgctt ccaggaaaag
     1141 cctctaagca tcaggtaaca tcaaatcgta ccccaaaccg acacaggtgg tcaggtagag
     1201 aataccaagg cgcttgagag aactcgggtg aaggaactag gcaaaatggt gccgtaactt
     1261 cgggagaagg cacgctgata tgtaggtgaa gtcccttgcg gatggagctg aaatcagtcg
     1321 aagataccag ctggctgcaa ctgtttatta aaaacacagc actgtgcaaa cacgaaagtg
     1381 gacgtatacg gtgtgacgcc tgcccggtgc cggaaggtta attgatgggg tcagcgcaag
     1441 cgaagctctt gatcgaagcc ccggtaaacg gcggccgtaa ctataacggt cctaaggtag
     1501 cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg cgtaatgatg gccaggctgt
     1561 ctccacccga gactcagtga aattgaactc gctgtgaaga tgcagtgtac ccgcggcaag
     1621 acggaaagac cccgtgaacc tttactatag cttgacactg aacattgagc cttgatgtgt
     1681 aggataggtg ggaggctttg aagtgtggac gccagtctgc atggagccgg ccttgaaata
     1741 ccacccttta atgtttgatg ttctaacgtg gacccgtgat ccgggttgcg gacagtgtct
     1801 ggtgggtagt ttgactgggg cggtctcctc ctaaagagta acggaggagc acgaaggttg
     1861 gctaatcctg gtcggacatc aggaggttag tgcaatggca taagccagct tgactgcgag
     1921 cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat ccggtggttc tgaatggaag
     1981 ggccatcgct caacggataa aaggtactcc ggggataaca ggctgatacc gcccaagagt
     2041 tcatatcgac ggcggtgttt ggcacctcga tgtcggctca tcacatcctg gggctgaagt
     2101 aggtcccaag ggtatggctg ttcgccattt aaagtggtac gcgagctggg tttagaacgt
     2161 cgtgagacag ttcggtccct atctgccgtg ggcgctggag aactgagggg ggctgctcct
     2221 agtacgagag gaccggagtg gacgcatcac tggtgttcgg gttgtcatgc caatggcact
     2281 gcccggtagc taaatgcgga agagataagt gctgaaagca tctaagcacg aaacttgccc
     2341 cgagatgagt tctccctgac cctttaaggg tcctgaagga acgttgaaga cgacgacgtt
     2401 gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa ccggtactaa tgaaccgtga
     2461 gg
//
LOCUS       DAZOZD010000402         2447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOZD010000402 DAZOZD010000000
VERSION     DAZOZD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.408"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(26..316)
                     /locus_tag="ACHUPB_004459"
     CDS             complement(26..316)
                     /locus_tag="ACHUPB_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0761074.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHUPB_004460"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="ACHUPB_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0761075.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="ACHUPB_004461"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="ACHUPB_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0761076.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHUPB_004462"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="ACHUPB_004462"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2447)
                     /locus_tag="ACHUPB_004463"
     CDS             complement(2172..>2447)
                     /locus_tag="ACHUPB_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0761077.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          576 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgacc
//
LOCUS       DAZOZD010000403         2437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOZD010000403 DAZOZD010000000
VERSION     DAZOZD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.409"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            221..337
                     /locus_tag="ACHUPB_004464"
                     /pseudo
     CDS             221..337
                     /locus_tag="ACHUPB_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(442..518)
                     /locus_tag="ACHUPB_004465"
     tRNA            complement(442..518)
                     /locus_tag="ACHUPB_004465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(532..608)
                     /locus_tag="ACHUPB_004466"
     tRNA            complement(532..608)
                     /locus_tag="ACHUPB_004466"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Arg,seq:tcg)
     gene            complement(616..691)
                     /locus_tag="ACHUPB_004467"
     tRNA            complement(616..691)
                     /locus_tag="ACHUPB_004467"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Ile,seq:cat)
     gene            complement(732..1790)
                     /locus_tag="ACHUPB_004468"
     CDS             complement(732..1790)
                     /locus_tag="ACHUPB_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0761078.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1898..2138)
                     /locus_tag="ACHUPB_004469"
                     /pseudo
     CDS             complement(1898..2138)
                     /locus_tag="ACHUPB_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2363..>2437)
                     /locus_tag="ACHUPB_004470"
     CDS             complement(2363..>2437)
                     /locus_tag="ACHUPB_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0761079.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          585 a          698 c          560 g          594 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      421 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc
      541 atcggggact tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct
      601 aatggcggta tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
      661 tcgctcgctc tcaccactga gctaaagggc cgggagccgc ataataacga cgcgtaatta
      721 attcttcaat atcatccgtt ctggctgact aaatcctgta cttcccgaac cgtctgctca
      781 aaacgttcag tctccagctc aacgccaatt gcacgacgcc cgagcgccag tgccgctttc
      841 actgtcgaac ccaaccccat gaaaaaatct gcaatcaggt cacccggacg actgctagcg
      901 ctgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc gggatagaac
      961 tgcaccggtt tatgcgtcca cacatcggta tacggcacct gcaccgtcac gccaaaatac
     1021 cgccgcagat gtttatattc actcagcagc tccgcatact gccggttcag tgaggtatac
     1081 gtatccacca gctggtggtg gagcttttcc agttcaccac gccgatgctt ctcttctgcc
     1141 acccgggcaa acagcgcctg taatttcaga taatcgcttt cattcggcaa ctgccactga
     1201 ccggcactga accagtgcga caccatgttt ttctttcctg tggcatctgc aatctgtttt
     1261 gccgttatcc ccagggcagc tcgcgcatca cgaaagtaag aaatcagcgg ggccatcaca
     1321 tgctgtttca gtgcactgcc cttcgccgca tacccggcat ctttcggacg atacggcccc
     1381 tgataatgtt ccgcgaacag aatgcgctct gtggcgggga aatacgcccg caggctttcc
     1441 ttgttgcatc cgttccagcg tccggacggc ttcgcccaga taatatggtt cagcacactg
     1501 aagcgttcac gcatcatgat ttcgatatca gatgccaggc gatgaccaca gaacaggtaa
     1561 agacttccgg caggtttcag cacccgccag aactgcgcca gacactggtc cagccacttc
     1621 aggtaatcat cgtcgccctt ccactggtta tcccagccct caggcttcac tttaaagtac
     1681 ggcgggtccg tgactatcag gtcaacagaa ttttcgggta acgaccggat aaattccagg
     1741 cagtcggcgt tgattaactc acaactggat atttttacag tattaagcat ggatcattaa
     1801 gcctgtctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
     1861 tgaacccaac gcatgagcag atggctggtg ggtgccccta acacccacca gccgcccatt
     1921 taccacaaat aaaaaagcct tcactgcgga aggcgtctgt aacaaccgaa ctgatagtct
     1981 gccagacccg ccataaccag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
     2041 gtattctgcg ctatctcccc gactgtcgcc ggttcggtaa cgcttaattc attaaacacc
     2101 actctggcgg tttctgtcat atcctgctgt tttagcatgt cttttccttt tccggttaac
     2161 gtgacacacc aataactctt gtcgaaaaag ccagcaagct gaaagacagg tattcaccgc
     2221 caccagcgcg tttactgtac tgacgcgatt tcagtcataa aaaacccgct cggcggcggg
     2281 ttgtagaaac tcttctaacg tcaggcataa aacgcccatc gttatgacga atttaccaca
     2341 gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg
     2401 cttcttcaat atcaaaccgg gtcaccagcg catcata
//
LOCUS       DAZOZD010000404         2402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOZD010000404 DAZOZD010000000
VERSION     DAZOZD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.410"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            75..164
                     /locus_tag="ACHUPB_004471"
                     /pseudo
     CDS             75..164
                     /locus_tag="ACHUPB_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="ACHUPB_004472"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="ACHUPB_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFR0761080.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="ACHUPB_004473"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="ACHUPB_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="ACHUPB_004474"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="ACHUPB_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          618 g          613 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagccc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 ct
//
LOCUS       DAZOZD010000405         2368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOZD010000405 DAZOZD010000000
VERSION     DAZOZD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.411"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            113..715
                     /locus_tag="ACHUPB_004475"
     CDS             113..715
                     /locus_tag="ACHUPB_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR0761081.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(1012..1833)
                     /locus_tag="ACHUPB_004476"
     CDS             complement(1012..1833)
                     /locus_tag="ACHUPB_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR0761082.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(1944..2240)
                     /locus_tag="ACHUPB_004477"
     CDS             complement(1944..2240)
                     /locus_tag="ACHUPB_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761083.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            complement(2264..>2368)
                     /locus_tag="ACHUPB_004478"
     CDS             complement(2264..>2368)
                     /locus_tag="ACHUPB_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0761084.1"
                     /translation="KVLKQVMVNTGGNKRQVPAKRQNRGSRNSTDDGG"
BASE COUNT          673 a          541 c          504 g          650 t
ORIGIN      
        1 agctgcgtta acgacgctgc tttaaaaaaa tcagatttaa acaatataaa tccacaaata
       61 caactcaatg atattaaaga taaatcagca aaaacttgtt tttgcgtagt gtgtggtgct
      121 tttggtggtg agaaccacca acctgttgag cctttttgtg gagtgggtta aattatttac
      181 ggataaagtc accagaggtg gaaaaatgaa aaaatggatg ttagcaatct gcctgatgtt
      241 tataaatggg atctgcgaag ccgccgattg ctttgatctt gcaggtcggg attacaaaat
      301 agacccggat ttactgagag cgatatcatg gaaagaatcc cgttaccggg ttaatgccat
      361 cggtattaat ccggtaacgg gatatggcag cggactgatg caggtagatt cccagcattt
      421 taacgaactg gcccgctatg gaattaagcc ggaacatctg acaacagatc cctgtatgaa
      481 catttatacc ggtgcttatt atctggcaat agcctttaaa aaatggggcg tctcctggga
      541 ggccgttggt gcatacaatg ccggattcag gaagaccgaa cgccagaacc agagacgtct
      601 tgcctacgca tcagaggttt accggattta tacctggata aagagcagta aaggcatccg
      661 ggttccgacc acgaagaaat cactttccca aattaacagt gtgcagaaaa attaatattg
      721 tatacattgt caaataccgg ttatgccgcg acagcggcat ggaaggccac acacaaaaat
      781 atcatatgag tgataatgct gactggccga agccccgcaa agcgggatcc catcttatat
      841 atatccggta actgcgcagc ctcctgccag accatttttc gcgggcacaa tatttacaga
      901 ctaccgaaaa gccagttgat gaaattgagg catatcagac gacacagcaa tgccgcagga
      961 tgataatata agtaaagccc cgaaaaattt ttcggggctt tacttatatt atcatgactt
     1021 ccactccctg aactgttcgg caatcatcca taatgctttg ttgattcgga tatcaccgtc
     1081 aatgcctgta atggctcttg ttctggtatt tttaccggag gcacttcgcc ccgataatcc
     1141 accttttatc atattctcct gaacccgctg ccaggttgtc cagagatcgt tctgtttatc
     1201 ctcccaacgg cggggagtaa ttatttgttc tggcgtcact ggcgttttat tttcatcttc
     1261 gtacctggcc atcaatgcag ctctgccaaa taaatgttgt tcgtcactgt taagatgtat
     1321 ctctttcatt gtctccatat tttcagtgac tttatcaaag acaccgagca cttcatacgc
     1381 tccctcaata acctgcccga caatatcacc tttatgtgga acgcggattt cgccaaaatt
     1441 attcccacac accaggccat ttgtgcagac aaaacgaaaa attcctggga tcatctgata
     1501 actggatgaa ccatcatgtg aattaagcag gataatttca ggaacttctt tcccgttaat
     1561 atgcccttcc ctgcgaagac gtaacatatg tttactgtat tcgcggcgtc ccaaatcacg
     1621 aacccgactc tgacaggcaa agaatggctg gaaaccttca tcacgtaact tattgatgat
     1681 attgatggtt gggatatatg tataacgctc actccgggat tcatgtttat caccagaaaa
     1741 gacagatgga acaaactgca ttaattcgtc atccgttaac gggcgctcac ggcgaataga
     1801 attatatcga ccaaaacggg aagctaatcg cataacagta actcctttat gaaggcataa
     1861 aattaataaa agttttttcg cttaatatca tcccccctcg gttatcgtta acaaaaagac
     1921 aacaattccg aggggaagga ccatcaaatc aggtcagttc gggactgacc aacctgccga
     1981 gtgaaaagca gtaacagcgc gatcaactca tcagcgtttt ttctgacaag aggagtatga
     2041 ttctccaggc tatccagtaa gtcatgcagc tcattattga cctggtggag caccttaaag
     2101 agtgcaccag aatcccggca gctctgatat gcaagatttg cctgaacagc acgattctgc
     2161 atgatcccga tttgtgcgat tgcgtctttc tgctgatcgc ctgttggctg ataagtggtg
     2221 ttaaaagaaa atgcgctcat aattcctcct gtatctgatg ttttcagccc ccatcatctg
     2281 ttgagtttcg ggagccgcgg ttttgacgtt ttgcggggac ctgccttttg ttccctccgg
     2341 tattcaccat cacctgtttg agaacctt
//
LOCUS       DAZOZD010000406         2352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOZD010000406 DAZOZD010000000
VERSION     DAZOZD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.412"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1511)
                     /locus_tag="ACHUPB_004479"
     CDS             complement(<1..1511)
                     /locus_tag="ACHUPB_004479"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR0761085.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
     gene            complement(1532..2158)
                     /locus_tag="ACHUPB_004480"
     CDS             complement(1532..2158)
                     /locus_tag="ACHUPB_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0761086.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT          604 a          616 c          502 g          630 t
ORIGIN      
        1 ataaagttga tatgggtatg cgtcatgcag tcacggttga acatacagtt aacaatggaa
       61 ccgacctgcg ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt
      121 ttatttttcg cgtagcccca gtactcttca cccaaattcc agcgttctgc gatggcatat
      181 gaaccatcag ccaggtgact cagctcaccc acacgatgcg ccagacggta gtaaaaatct
      241 ttaattttaa tgaagttaac gtcacccgct tccagttgat cccagcgaat ttccgcatac
      301 tcttcagcag gcagaacacg cttgaacaca cctttgctgt aacaataggt aaagtcgcga
      361 tgcaactgcc cgtagttaca ccataggccg tagtcatcga acagattcag acccaccagg
      421 ttaccaatca cacgaccatc gtctttatct tcaaaatctt tcggaccgtt cgggaagatg
      481 gtggtatgga caaagttggc tacacaagtg ttaccacctg tgctgggcac gccaaactct
      541 ttcacccgag gaatattcat ttgggtcata caacggatcg ggcaagagtg gcagccactc
      601 atttttactg tgtatttctc tgccgccgga cctaagtcaa aaacggattt ataggtacga
      661 aagccgaccg tgttctgatt gcctggcgga atttcccccg tttcaatcgg accaccttca
      721 gccgcgcccc aaaacagccc tttacgtgct gtccaacgtg acttgggatc tgaatactct
      781 gcccacgatt gcggcgtact tggcacgaca tggttgttat tcgcaccaat aagttcagtc
      841 atcatgtaat cgttgagacg cttcatctcc tgacgatcgg caatgttgac ccctttcgtc
      901 ccttcaaccg caatcgcttt caggtttttc gaacccatta ttgcgccagt tcccgcaccg
      961 ccgctgtggt tacggctatt caacatgcca gagagaggaa caaggttttc cccagcctga
     1021 ccaatagccg ccacacaggt ttccggactg gtcaatcgac aaatttcttc cgtcgttgcg
     1081 cgcgtccctt ttccccataa gaaatcggct ttttccaggc taactttgtc atctttaatc
     1141 ttcagccata ccggtgattt cgccttccct tcgataataa tgacgtcgta tccagcgaat
     1201 ttcatttgcg ctgcaaaaaa gccacccata tgggcatcga cgactaaatt tcctttggta
     1261 aaagtagaaa gtgaggtgat atttacgcga gaactacagg gggcaccaga tccagttaat
     1321 gggccggtag caaagactaa tttattcgct tcatcgaaag gtttcgtgcc tggcggtact
     1381 tcgtcataca taattttgta gccgaagccc atgccaccga caaaactttt aaacttactg
     1441 gaatcttcga gggtaatatt tcctgtcgtg agattgactc ttaatatatt acctgtccaa
     1501 ccgttagcca tgattttttc ctttgcaaga ttcacacagt aatatctttc cactcgataa
     1561 tttttaacgc ccctgttggg caggcgtttg cgcattcacc gcataacaca catttcgagg
     1621 attttttact ttcggtattt acggtggcca tcatccacgg gcatgccgta gtacaggcgc
     1681 tacagccaat acagcgttta tgatcgacgg taatacagcc ttctttctgc tgccaggtaa
     1741 tcgcaccaat cgggcagacg ttcatgcatt gcggctcttt gcattgacgg caggtgtccg
     1801 cggtatagtt gagatcgcca tacaggccgc cgccagagcc aaccccgttg tcgccaaaga
     1861 aataattgcg atggattttg atacgggaga agaatgtccc tactgagcca tcgttgaagt
     1921 tggtacaaga gatttcacag cggtggcaac ccgtgcatcg cgctcgctgg gtcaccagca
     1981 cccctttggg tgtatttatc aaccctactg tgccgctatc aatatcttcc tgtttgcaac
     2041 ccaaaagtga cagtaacgca ggggcaattg ttagaccagc aaggcctttt cctgatatac
     2101 gcaagaattc taatcgcgtt aagccaatat ctaatagtgg acgatcaacc gggttcattt
     2161 atttcgcatc ctcttcgcaa acacagtgct gacaattcag cagcctgata ttaagcattc
     2221 aactaatacg ctgctgactc tgtccccaac accagatatt ttccctaacc cgatagtgta
     2281 tgagaaaaga tttaacgccg ataaaccaat cctttatggt taggagaaaa aataatgtga
     2341 ttattctgag cc
//
LOCUS       DAZOZD010000407         2313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOZD010000407 DAZOZD010000000
VERSION     DAZOZD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.413"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..207)
                     /locus_tag="ACHUPB_004481"
     CDS             complement(<1..207)
                     /locus_tag="ACHUPB_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761087.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITS"
     gene            472..927
                     /locus_tag="ACHUPB_004482"
     CDS             472..927
                     /locus_tag="ACHUPB_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR0761088.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            927..1097
                     /locus_tag="ACHUPB_004483"
     CDS             927..1097
                     /locus_tag="ACHUPB_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR0761089.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1090..1380
                     /locus_tag="ACHUPB_004484"
     CDS             1090..1380
                     /locus_tag="ACHUPB_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0761090.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1377..1739
                     /gene="rusA"
                     /locus_tag="ACHUPB_004485"
     CDS             1377..1739
                     /gene="rusA"
                     /locus_tag="ACHUPB_004485"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR0761091.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1736..1876
                     /locus_tag="ACHUPB_004486"
     CDS             1736..1876
                     /locus_tag="ACHUPB_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR0761092.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1962..>2313
                     /gene="quuD"
                     /locus_tag="ACHUPB_004487"
     CDS             1962..>2313
                     /gene="quuD"
                     /locus_tag="ACHUPB_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR0761093.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRL"
BASE COUNT          601 a          525 c          587 g          600 t
ORIGIN      
        1 cgaggtaatt gcctcaccta atttagtaaa attaccagca atagatgatt ttctttttcc
       61 acttctattc aaagtggctt taattacacc accaccatcc ttagagttaa ataattgaat
      121 gtccactcca gcagttggtt gtatatattt tgaaagctct ttcttgaact tgttaatata
      181 tttatctttg ttttctttgt tggtcattgg ttagatccat agttctggct gtgattaggt
      241 gaaagtatac aaggttttta aagcgaagat cactgttttg gcttcaagat agttttgatt
      301 tatcgtaatc aatccgccat aatcctctca tcggagcctg aacaactccg gtgacttctg
      361 cgcatttaag gggacttaaa tgcgaccaca atctgaactc atcaccttgt cacagatgca
      421 gaaatgcacc tgcgattttc tgcattctgc ggtttccgtt agggaggccg tatgactctg
      481 ccagtagacg gcatcaaact ccatcgcggt aacttcgcgg ccatcggcca gcagattcag
      541 ccattgctgg atgccgggca atgcttacgc ctgcaggtta agccgtggcg cgagaagcgc
      601 agcctgtcgc agaacgcgct cagccacatg tggtacacgg agatcagcga atatctcatc
      661 gcccgcggca agaccttcgc tacgcctgag tgggtcaaag acgcgatgaa gcacacctat
      721 ctcggctacg aaagcaaaga ccgtgtagat gtcgtgtccg gcgaggtcac caccgtccaa
      781 tccctccgcc atacgtcaga gctggaaacc ggcgagatgt acatcttcct gtgcaaagtc
      841 gaagcctggg cgatgaatat cggctgccac ctgacaattc cgcagagctg tgaataccag
      901 cagctgcgcg ataagcagga ggcgtaatgg ctacaccgct tattcgtgtc atgaacgggc
      961 acatctacag agtatcaaat cgtcgtaagc gtaagcctga gctgaagcca tccgaaatac
     1021 caacactgct cggatatacc gctagcctgg ttgataaaaa atggttgcga ctggcagcaa
     1081 ggaggaatca tggctgattt gagaaaagca gcgcgtggtc gggaatgcca ggtaagaatc
     1141 cctggcgtat gtaatggcaa tcctgaaacg tcagtactgg cacatatccg gctggctgga
     1201 ttgtgcggta ccggtatcaa accgccagac ctgattgcca ccattgcatg ttctgcctgt
     1261 cacgacgaaa tcgaccgccg cacatatttt gtcgatgctg agtatgcaaa agaatgcgcg
     1321 ctggaaggta tggcgagaac gcaggttatc tggctgaaag agggggttat taaggcgtga
     1381 atacctacag catcacatta ccctggcctc cgagcaataa ccgctattac cgccataatc
     1441 gcgggcgcac acacatcagc gcagaagggc aggcataccg cgataacgtc acccgaatca
     1501 ttaaaaacgc aatgctggat atcggcctgg ctatgccagt gaaaatccgt attgagtgcc
     1561 acatgccgga tcgccgtcgc cgtgacctgg ataatctgca aaaagccgct tttgacgcac
     1621 tcaccaaagc aggtttctgg ctggatgatg ctcaggtcgt tgattaccgc gttgtgaaga
     1681 tgcctgttac caaaggtggg aggctggaac tgaccatcac cgaaatgggg aatgaatgat
     1741 gtttgagttt tatatggcag aatttcttcg ccaccgctgg gggcatctgc gcttatatcg
     1801 tttccccggt tctgttttga ccgattaccg aatactgaag aattacgcca aaaccctgac
     1861 aggagcagga gtatgaagtc agagataaca atcaactaat actgttttgt tgatttttgc
     1921 ttgtaattgg cgttctggtc tgatttttgt ggagtaagtt gatgcgtgat attcagatgg
     1981 ttcttgagcg ttggggagcg tgggcggcta ataatcatga agatgtgacc tggtcgtcca
     2041 ttgccgccgg ttttaaggga ttaattactt caaaagtaaa atctcgcccg caatgttgtg
     2101 acgatgacgc gatgattatt tgcgggtgca tggcccgtct gaaaaagaac aacagcgatt
     2161 tgcacgattt attagtagat tattatgtag tcggtatgac attcatgtca ctggcaggta
     2221 agcattgctg ctctgatggt tatatcggga aaaggttaca gaaggctgag ggcataattg
     2281 aagggatgtt aatggcatta gatatccggt tag
//
LOCUS       DAZOZD010000408         2296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOZD010000408 DAZOZD010000000
VERSION     DAZOZD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.414"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..431)
                     /locus_tag="ACHUPB_004488"
     CDS             complement(<1..431)
                     /locus_tag="ACHUPB_004488"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0761094.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            533..757
                     /locus_tag="ACHUPB_004489"
     CDS             533..757
                     /locus_tag="ACHUPB_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0761095.1"
                     /translation="MQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            869..1132
                     /locus_tag="ACHUPB_004490"
                     /pseudo
     CDS             869..1132
                     /locus_tag="ACHUPB_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(1169..1726)
                     /locus_tag="ACHUPB_004491"
     CDS             complement(1169..1726)
                     /locus_tag="ACHUPB_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0761096.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1723..1998)
                     /locus_tag="ACHUPB_004492"
     CDS             complement(1723..1998)
                     /locus_tag="ACHUPB_004492"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR0761097.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            2183..2296
                     /locus_tag="ACHUPB_004493"
                     /pseudo
     CDS             2183..2296
                     /locus_tag="ACHUPB_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          651 a          471 c          543 g          631 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tagtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat ttagtgagta
      601 tattgcgcca ctgcaggatg ctgtagatct ggaaatcgca acggaggaag aaagatcgtt
      661 gctggaggca tggaataaat atcgggtatt gttgaatcgt gttgatacat caactgcacc
      721 tgatattgag tggcctgcaa atcctgtcag ggagtaatca ttgggattat gccgcagaca
      781 cgtcgtatgc aggaacgtgc tgcggttagt ttgtgagctt tcgatagtgg ttgttatttt
      841 tgcccttatt tgttccggag gccatggttc aatggtccgt ctgccccctg tggtgatgtc
      901 agcaaaatca gccactgcgc gaaccacaat agcccgggaa gatgctgaag atcaccaggt
      961 aaagctgtca gcgcagaaac tggaagaact gctcgcatca atggttaagg atgaggttga
     1021 tcgcaatgat gggatttatt gacgtcagcg agagcagaag gaagaactga ataacctgaa
     1081 tgatttacgc tcaatcagag cgatggcgat tagcggcaat cacgctcgat aaaattaacc
     1141 tcggtgatca tccggggttt tgcattcatt aaaaccgcat caacctttcc accaattgtt
     1201 ctttacgggc aacgatccac ccatgttgct ccagataaaa tttaaaccgt tccagagtgc
     1261 ataccatcgc atcggcgggg actttttctg tgaactcgac ctgaccgtgt ttatcgaagt
     1321 ggatcagtaa tgcgcatcca ttatttgggg cgggggagtt ttgtgctgca ggaggacgtt
     1381 tgtggagttc ttcttccatt gcgttaaatt tttcaatgta agcttctttt agtctggctg
     1441 cttttttgcc agtaaatccc attacgagga acatgaagcc gtcttttgtg atcaggtagc
     1501 atcgtgattc acgttctgcg ccgttgccaa tttcgacagt ctgaacatcg gccgaaaagt
     1561 tggctgatgt aaattcggga gagcagtcga gtgtgtcaat cttagctaaa acgtgtttat
     1621 gttgtttatc gaagtagtcg gcaacagctt gagaggtggt aaccaggcga ccatctttga
     1681 aaaagacttt tggtgactcg ttagaaaaag ttatagctgt attcatatgt gatgactcca
     1741 attaattgga tgatcaccac ctctgacgcc aatcagggag gtggtgagac gtacagggtt
     1801 ggcgtaaccg gtcatcacaa aacccggcgc ggatttctcc gcccctgcac gccccaccat
     1861 aatgcgaatg tggcaatgct taacgcaaaa aaaaccgcaa acgcggctat gcgttgtgat
     1921 gttatccgag acgccaatcc cgacaaccga ttttgcggtt gtgtgaggaa tatagccccg
     1981 gagaacaaaa actgtcaacg ggcaaaaaaa tttccgttat caggaatgta taaaattaaa
     2041 gcgatcgagc aaaaaatcct ttctgcaaaa ctaaaaaaga tttcaataaa atcaggcagt
     2101 taaaaacacg gaaaacggta taaaaaagcg cctcaaaatc ctgttttttc tttcagatac
     2161 aatgtcttat tgcatttttt aaaacatgtc agtgtggtac atggatatcg ataccatcgc
     2221 caattataaa ctgggcggtg cacagcaaca tgatagcgta cgcctcgatc cgtgggtgtt
     2281 tatgttctca gcagga
//
LOCUS       DAZOZD010000409         2292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOZD010000409 DAZOZD010000000
VERSION     DAZOZD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.415"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHUPB_004494"
     CDS             complement(<1..502)
                     /locus_tag="ACHUPB_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0761098.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="ACHUPB_004495"
     tRNA            complement(688..772)
                     /locus_tag="ACHUPB_004495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="ACHUPB_004496"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="ACHUPB_004496"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0761099.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..>2292)
                     /gene="idnK"
                     /locus_tag="ACHUPB_004497"
     CDS             complement(1991..>2292)
                     /gene="idnK"
                     /locus_tag="ACHUPB_004497"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconate kinase"
                     /protein_id="HFR0761100.1"
                     /translation="YRDILRKGSPHVHFLWLDGDYETILARMQRRAGHFMPVALLKSQ
                     FEALERPQADEQDIVRIDINHDIANVTEQCRQAVLAIRQDRICAKGGSASDQRCE"
BASE COUNT          546 a          578 c          584 g          584 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ct
//
LOCUS       DAZOZD010000410         2260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOZD010000410 DAZOZD010000000
VERSION     DAZOZD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.416"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(123..1127)
                     /gene="argF"
                     /locus_tag="ACHUPB_004498"
     CDS             complement(123..1127)
                     /gene="argF"
                     /locus_tag="ACHUPB_004498"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0761101.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1289..1705
                     /gene="rraB"
                     /locus_tag="ACHUPB_004499"
     CDS             1289..1705
                     /gene="rraB"
                     /locus_tag="ACHUPB_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0761102.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1751..2254)
                     /locus_tag="ACHUPB_004500"
     CDS             complement(1751..2254)
                     /locus_tag="ACHUPB_004500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0761103.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          573 a          559 c          551 g          577 t
ORIGIN      
        1 cctccggaag atttacgtat agcaataaaa aaattggggt ggaataattg aactatcact
       61 gcataaaata atgtgacgcc gggatgtttt tgtatttccc ggcatcttta tagcgatatc
      121 aattatttac tgagcgtcgc gaccatcacc gctttgatgg tatgcatgcg gttttccgcc
      181 tgatcaaata caatgctggc ggcagattcg aagacctcat cagtcacttc cattccgcca
      241 tgtaggccaa actcttccgc catttttttg ccaagcgtcg tttggtcgtc atgaaacgcg
      301 ggcaggcagt ggaggaattt gacctccggg ttaccggtca actgcatcat cttgctgttc
      361 acttgataat cacgcagcaa tgcaatgcgt tcagcccatt tctcttttgc ttcacccatc
      421 gacacccata catcggtata gataaagtca gcaccttcaa ctcctttagc tacatcttcc
      481 gtcagcgtaa tattcccgcc attttgttgt gccagggcgc ggcattccgt aaccagcgca
      541 gcttccggcc agcatgcctg tggcgcgacc agacgcaaat ccagaccggt aagcgctgca
      601 gcttcgagca tcgaattgcc catgttgtta cgtgcgtcac ctgcatagac tagcgtcatt
      661 tcgttgaacg ttttgccggg caaatgctcc tgcatggtga ggagatccgc cagcagctgc
      721 gtggggtgaa actcattggt caggccgttc cataccggca cgccagcgta ttccgccagt
      781 gtttcgacaa tctcctgacc atagccgcga tactgaatac cgtcatacat gcgaccaagc
      841 acgcgggcag tgtctttaat cgactcttta tgaccaattt ggctgccgct tgggccgaga
      901 taagtaacac gagccccctg gtcatatgcg gcaacttcga aagagcatcg ggtacgggtc
      961 gagtcttttt cgaagatgag cgcgatgttt ttaccagtga gtttggcttc ttctttaccg
     1021 cttttcttat cggctttcag cttcgcggct aactgcagca ggctgttgag ttcagctggc
     1081 gtgaaatcga gtaatttcag gaaatgctta tgataaaacc cggacatagg tccctcctgt
     1141 ggctaacgcc ttaatgaatt aaaattcaat ttatatggat gattattcat ttgcaagtct
     1201 aaagcataaa tctttatcac aaaggtggag gcaatgtcag tggtgtgtga caataagagt
     1261 atcggcagga cattaagagg aatgagccat ggcaaacccg gaacaactgg aagaacagcg
     1321 tgaagaaaca cgtttgatta ttgaagaatt actggaagat ggcagcgatc cggacgcgtt
     1381 gtacaccatc gaacaccatc tttccgcaga cgatctggaa actctggaaa aagcggcagt
     1441 tgaagcgttt aaactcggtt acgaagtgac cgatccagaa gagctggaag ttgaagatgg
     1501 tgatatcgtg atttgctgcg acatcctcag cgagtgcgcg ttgaatgccg atctgatcga
     1561 tgcccaggtt gaacaactga tgacgctggc ggagaaattt gacgttgagt acgacggttg
     1621 gggcacttac tttgaagatc ctaacggcga agatggcgac gatgaagatt ttgtcgatga
     1681 agacgatgac ggggttcgcc actaattaat tgattaaaat gcccgatagc acatactagc
     1741 gggcattttt ttagagttcg cgtagcatcc gcacttcaca atcgacatgg cccgtgcagc
     1801 caagcgcata gtcgatatgc tcaaagccca aatgctcata aagcgcaatg gcttccttta
     1861 aaaaagcggt cgtttccaga tagcagcgtt tgaagcccat ctctcgtgcc tgctccatcg
     1921 ccattaaggc cagttttttc gctagccctt taccgcgaat agccggaaga aaatacatct
     1981 tttgcagttc gcaaatgtcc gactcactcc cggttaatgg cgcaatccca ccgccgccga
     2041 ccacttcacc ctcgtactca accacccaat atgcatggcc aggctgacta tacacttgat
     2101 acagctcgtc aagattcgga tcggcgacgg tgtagccttt atcagcggta agaccgtatt
     2161 cggcggatac ctgacgaatg acgcgggcga tagcaggatt atccagcagc gtcaggcgac
     2221 gcattacagg tgattgcggc gcaatgttat tcatagttta
//
LOCUS       DAZOZD010000411         2201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOZD010000411 DAZOZD010000000
VERSION     DAZOZD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.417"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(263..463)
                     /locus_tag="ACHUPB_004501"
     CDS             complement(263..463)
                     /locus_tag="ACHUPB_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761104.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(463..735)
                     /locus_tag="ACHUPB_004502"
     CDS             complement(463..735)
                     /locus_tag="ACHUPB_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0761105.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(738..1016)
                     /locus_tag="ACHUPB_004503"
     CDS             complement(738..1016)
                     /locus_tag="ACHUPB_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761106.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1017..1574)
                     /locus_tag="ACHUPB_004504"
     CDS             complement(1017..1574)
                     /locus_tag="ACHUPB_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761107.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          599 a          447 c          422 g          733 t
ORIGIN      
        1 aattgataca ccggaatcag cgaaagcctg caccatcata gcagtgtcgc tatcaatgaa
       61 aatctgtaat tcatattcgt tcatttttaa aatctccttt ttaccagacc tcctcaatcg
      121 ctctgtaacg agctaaacag tctttttcag gggtagcctg tacagtggta ttacccaccc
      181 taaaataaga tagttttttg cgttagaatg cgattgagag cgtctgataa gctgtttttc
      241 taatttctct taaagtctcg gactaccaat aacctaattc cgctttattt cgttcaaacc
      301 attccgctgt aactgctgtc tttagtgttg cgtttgggct ggtggcatga atgaagatat
      361 cttttggatt gcaagcatca ccgaaaccac cttctttgat gagcaggtct gatacatgct
      421 taatcatttt atctgtcaaa tattcgaatt taatacataa cattaaatct ccaggagtaa
      481 attgtttggg tggcaatttc ttttattacc atccctgaag ataatctttt tagttgcctg
      541 aacttcatga tgcaaaaacc agatgacata cttcagataa taatcacgag tgccataacg
      601 aatcattgga ttaccgtaag catcatctct tgtcttaacc tctccaccat caatccattc
      661 aagcattcct gtctcataat taatcatgaa gtaagttgca aagaaccgtt taatatcatc
      721 tcgccattgc ttcataatca gacctcctga gacagtttgt gaattttgat acttagtaac
      781 cttgctaaat ccagtccaaa agcatttaag gatttcagac tatcaaggtc atcttgctta
      841 atcctgatat ggtgtcttgc agcctctaag cgactattaa gcaaatcctg ttcgttaata
      901 tcacggttaa ttgcaagctt catcactgct gagtgtggca gacctgtaat gtttttgatt
      961 cggttcagtt catcaaaggc tgtctttgta actctggtta atgtgtgttt attcatttaa
     1021 aaatcctctg taactaaata ggggtcaaac tcgaaatctg gttgctcgtt ttccattggc
     1081 tcaatattcc caacggcaat atgatgttca acaatgccaa ttaatttcgc gcacatcaca
     1141 gcaagcaaag cgcaatattt atcgcttggt aattcgtaac taagtctggt tgcctgtcgc
     1201 tgaagatgat ttgtcacggt ggatgcgtta atttcgtcaa gattctttag cagataaaat
     1261 agttttttgt tgtatttcgg gtggccttca cttctacaga acccaataaa aacatcatca
     1321 agcttgcttg gtaactgctc gtaactgtca aagttagcca gataattttt aatagtattc
     1381 tttgttgttt tgcgtactgg cataaatctg tctcgtggcg taatgctacg aatgaccgct
     1441 tcaatgttct tacgttcgga tttgctaaag gttgggactt cgtagctggt ggggttgttt
     1501 gtgtaagcgt aggagttgtg cttgttgcgt tctcttgatg cctgaagcat cgctctaaat
     1561 tctgaatact gcattcatgt tctgactctc ataagatgtt attattctgt taacctatat
     1621 acaatattag cattttcaaa gactaatgtc aagtattttt aacattttcg taacatctgt
     1681 ggtataatgg gtttatgggg agagtaaaat atgaggcgtg gctatgtatt tggtcgcata
     1741 ttttgcattg cacacagacc tcttcaatcg ccctgtaacg agttaaacga cctcttcagg
     1801 tcaactcata aggtcaggta ctttttgtgt cagaatgcga ttgagagcgt cttacaagga
     1861 aaactaatgg taaatctccc tcctgagctg gggtcacctg atgtatggac tggtggaggt
     1921 attgggctga ttacaggcct gctacttcgc tacgtcctcg tcagagtgct tgttgtcttt
     1981 aaactgttcg gtcgtacatg gacgcttgac cttaaggata acgaccacac agacccccgt
     2041 taaactcctt caaaacccct gcacgagatg acagatagaa gtctatggta caggggtgtc
     2101 tttagcattt tgtgctatct attaaaaaaa tagcttgaca gaaccagaaa gccgtatcta
     2161 tcaagcattc acctctctga cagtaatttc taaaagttct t
//
LOCUS       DAZOZD010000412         2187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOZD010000412 DAZOZD010000000
VERSION     DAZOZD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.418"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            174..2159
                     /gene="yjcS"
                     /locus_tag="ACHUPB_004505"
     CDS             174..2159
                     /gene="yjcS"
                     /locus_tag="ACHUPB_004505"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR0761108.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          584 a          575 c          534 g          494 t
ORIGIN      
        1 aataaattcg cgtaattttc cctcttcagc acaaatgcct aataaaatgg atcgttcagc
       61 tcacacttcc acttattaaa gtcgcaaatt cccaattatg ataaccccag ctttaaatga
      121 cgtattttgc atggattaca tatttacgac ataaatgata aatggagatt tacatgaata
      181 actctcggtt attccgtttg agcagaattg ttattgcgtt aactgccgcc agcggcatga
      241 tggtaaatac cgctaacgcg aaagaggaag cgaaagccgc cactcaatat acccaacagg
      301 ttaatcagaa ttacgccaaa tcattaccgt ttagcgatcg tcaggatttt gacgacgctc
      361 agcgtggatt tatcgctccg ctgctggatg aaggtattct gcgcgatgct aatggcaaac
      421 catattatcg cggggaagat tataaatttg atatcaatgc gcccgcgccc gaaaccgtta
      481 accctagcct gtggcgtcag tcgcagttaa acggtatttc cggcctgttt aaagtcaccg
      541 acagaatgta ccaggttcgc ggtcaggata tctccaacat cacctttatc gaaggtgata
      601 ccgggattat tgtcatcgac ccgctggtga cgccaccgag cgcaaaagcc gcccttgacc
      661 tttacttcca aaatcgcccg caaaaaccga ttgttgcggt tatttatacc cacagccata
      721 ccgaccacta tggcggcgtg aaaggcatta tctctgaagc tgatgtgaaa tccggtaaag
      781 tgcaggttat cgcccctgct ggctttatgg acgaagccat cagcgaaaac gtactggcgg
      841 gtaatattat gagccgccgt gcactttact cctacggcct gctgctggcg cataaccctc
      901 agggtaacat cggcaatggt cttggcgtaa cgctggcatc gggctacccg agcatcatcg
      961 caccgaacaa aaccatcacc aaaaccggtg agaagatgat tatcgacggc ctggagtttg
     1021 acttcctgat gaccccaggt agcgaagcac cagccgaaat gcacttctat attccggccc
     1081 tgaaagcgct gtgtaccgcc gagaacgcca cgcataccct gcacaacttc tacactctgc
     1141 gcggcgcgaa aacccgcgac accagcaagt ggaccgagta tctgaacgaa acgctggata
     1201 tgtggggtaa cgacgcggaa gtcctgttta tgccgcacac ctggccggtc tggggcaata
     1261 agcatatcaa tgattatatt ggtaaatatc gcgatactat caagtacatt cacgaccaga
     1321 ccctgcacct ggcgaaccag ggctacacca tgaatgaaat cggcgacatg attaaactgc
     1381 cgcctgcact tgccaataac tgggccagcc gtggctatta cggttctgtc agccacaacg
     1441 cccgcgcggt gtataacttc tatcttggct attacgacgg taacccggct aacctgcatc
     1501 cgtatggtca ggtggagatg ggtaaacgtt acgtgcaggc gctgggcggt tctgcccgtg
     1561 tcatcaacct ggcgcaagaa gcgaacaagc aaggtgatta ccgctggtcg gcagaactgc
     1621 tgaaacaggt gattgccgcc aacccgggtg accaggtcgc gaagaatctg caagcgaata
     1681 actttgaaca gctgggctat caggccgagt ccgccacctg gcgcggtttc tacctgaccg
     1741 gcgcgaaaga gctgcgcgaa ggggtgcata agttcagcca cggcaccatc ggttccccgg
     1801 acaccattcg cgggatgtcg gtcgaaatgc tgttcgactt tatgtccgtt cgcctcgata
     1861 gcgcgaaagc cgcgggtaaa aatatcagcc tgaacttcaa tatgagcaat ggcgataacc
     1921 tcaacctgac gctgaacgat agcgtgctta actaccgtaa aacactgcaa tcccaagctg
     1981 acgcctcttt ctacatcagc cgtgaagatc tgcacgccgt gctgaccggg caagccaaaa
     2041 tggcggatct ggtaaaagcg aagaaagcca aaattattgg caatggcgcg aaactggaag
     2101 aaattatcgc ctgtctggat aatttcgatt tgtgggtgaa tatcgtaacc ccaaattaat
     2161 gtcactcgct tttgggggcg ctgaagc
//
LOCUS       DAZOZD010000413         2180 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOZD010000413 DAZOZD010000000
VERSION     DAZOZD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.419"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..678
                     /locus_tag="ACHUPB_004506"
     CDS             <1..678
                     /locus_tag="ACHUPB_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761109.1"
                     /translation="NPKYPGWISIYVLLDAPRLATLLINRHGGVLPPLLASAIQKLTG
                     TGAELVLSGSQWQSLPVLPADGTQVSFPYAGEWLTEDEIRAVLDAVHDAVRSVSCRVA
                     EDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDAILNRGAR
                     FSSVEMYLVSECVEHILSSGLACDVLRIPDEPSRRWFDRDILREVVLEARTEIRSMAD
                     ALAKIRK"
     gene            1094..1519
                     /locus_tag="ACHUPB_004507"
     CDS             1094..1519
                     /locus_tag="ACHUPB_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0761110.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            1566..1988
                     /locus_tag="ACHUPB_004508"
     CDS             1566..1988
                     /locus_tag="ACHUPB_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0761111.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            1985..2176
                     /locus_tag="ACHUPB_004509"
     CDS             1985..2176
                     /locus_tag="ACHUPB_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761112.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
BASE COUNT          448 a          575 c          685 g          472 t
ORIGIN      
        1 aacccgaagt atccgggctg gatcagcatt tatgttctgc tggatgcgcc ccggctggcg
       61 acgttactta tcaaccgtca cggcggcgta ctgccgccgc tcctggcctc tgccattcag
      121 aaactgaccg gaaccggggc ggaacttgtt ctgtccggca gtcagtggca gtcgctgccg
      181 gtacttccgg cagacggaac gcaggtgtct ttcccgtatg ccggagaatg gctgaccgaa
      241 gacgaaatca gggcagttct tgatgcggtg cacgatgcgg tacgcagtgt cagttgccgg
      301 gtggcagaag atgcgcggcg tatcagggcg gcgctgacca caaccggtca gacgttgctg
      361 acccgccaga cgcgccgctt tcgcctggtt gtgaaggaaa gcgatcaccc ctgctggctc
      421 gatgaagacg acgaaaacct gcccgtggtg ctcgatgcca tcctgaaccg gggcgctcgt
      481 ttttcgtcgg tggaaatgta tctggtcagt gagtgtgtgg agcatatcct gtccagtggg
      541 ctggcctgcg atgtgctgcg tatacctgat gaaccgtccc gtcgctggtt tgaccgggat
      601 attctgcggg aggtggtcct ggaagcccgg actgaaatcc gcagcatggc ggatgcactg
      661 gcgaaaatcc ggaaatgatg acgctggcgg ggaaaaatct ccgccttttc cagccggtcc
      721 ccggaaagag caaaaggatg tcgccgcagg ctccgcttta cccggccatg cggggcatgg
      781 ccttgtgggt tttcagttat gtggcctcag cgccgtgtgc gggctgtgcc gtgcctccat
      841 cgtagccggg cttctgcggg cgggttgcaa ggtaaggtga accgctgcgg tcacccggtc
      901 cctccttccc gtctgccgtg attttccggt cgttcacccg ctcagatttc gcggtctcac
      961 ccttcaactc ccgtcagccg tgcgcgtcag gctgcggctt cccttgcgcc cttgcaaccc
     1021 tgcaggcccg gcaaagatgg aggcacggca ccgcccggtg tcgcggaata tgtgaacaac
     1081 ggaggaaaac atcatgcaat atgcgaaacc tgtcactctg aacgttgaag agtgcgaccg
     1141 tctgtctttt ctgccttacc tgtttggcct ggattttctg tatgccgaag cgtctgtata
     1201 cgcgctggcg aaaaaaatga tgccggaata tgaaggcgga ttctggcact tcatccgcct
     1261 gccggacggt ggcggttaca tgatgccgga tggcgaccgt ttccacatgg tgaacggtgc
     1321 aaactggttt gaccgtaccg tgagtgctga tgccgcaggc atcatcctta cctcccttgt
     1381 gattaaccgc cagttgtggc tgtaccacga cagcggggat gcagggttga cacacctgta
     1441 caggatgcgt gatgcgcagt tgtggagcca catcgaattt caccccgaat gcaacgcgat
     1501 ttacgcggca cttgactgat taacggacgg ggcggcaatg ccgcccctgt aaaggagacg
     1561 agaacatgta ctgtactgtt aaagaaatca tccgcgatgt actggataca gacgtgccgg
     1621 acagtgaatg cgtttttgcc gtggtgctga cccgtgggga tgtgcgtcac atagcccagg
     1681 actggaacct gtcagacgat gagctggaaa ccgtcatgca gcggctggac gatgcttttg
     1741 tatatggcgc atgtgacaga gttgtttccg atattgtcaa tgaactgatg gaagaaaagc
     1801 gcgccagccg tcaggtgaca gtcccggcgg tgatgctgga aaaagtgatg gcgctggcag
     1861 gcagtgaaat gaagcgcctg tatgccgtcg ggagtgagaa cgggggcgac ggtgacgcat
     1921 tcgtcaggga ggaactcgaa gcaatggacg ttgtgttgca ggcgctggac ggggagacga
     1981 tgtcatgaat atcagcacag aaacccgcga aattctgcgt aattacaggg ccgtgattaa
     2041 tgcgcggcgt cgtgaaatgg ggcagaaacc gctcaccacc gcgcagattg ttgatgaaat
     2101 ctgcgatttt gtggcgaatc agcaggcggt tttcctgggc ggtcactaca tccttcaggg
     2161 cagcagaaac aggtgataca
//
LOCUS       DAZOZD010000414         2161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOZD010000414 DAZOZD010000000
VERSION     DAZOZD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.420"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            36..1229
                     /locus_tag="ACHUPB_004510"
     CDS             36..1229
                     /locus_tag="ACHUPB_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0761113.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            complement(1179..2105)
                     /locus_tag="ACHUPB_004511"
     CDS             complement(1179..2105)
                     /locus_tag="ACHUPB_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR0761114.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
BASE COUNT          482 a          522 c          527 g          630 t
ORIGIN      
        1 taaacacaat taatacatgg agttgaaaca ttataatgtc accagggatc gaagatacgc
       61 cccaaaaaac actgtcctgc tggccacttg cgttcagtgc cgggcttctc ggtatcggac
      121 agaacggtct gctggttgtg ctccctgttc tggtcataca gacaaatctg agtctgtctg
      181 tatgggctgc cctgctgatg cttggctcaa tgctgtttct gccatcgtcc ccgtggtggg
      241 gaaagcaaat ttcccgtact ggcagtaaac ctgtggtgtt gtgggctctg ggaggatatg
      301 gcataagctt taccctgcta gggctgggaa gcgtgctgat ggctaccagc gccataacaa
      361 cagcggtggg actgggaata ttaatcatcg cccggatcgc ctacgggctg accgtgtcgg
      421 caatggtgcc agcctgtcag gtctgggcat tgcagagagc tggagaaggg aatcgcatgg
      481 ccgctctggc aaccatcagc tccggcctga gttgcggcag gctattcggg ccgctgtgcg
      541 cggcagcaat gctggccatt catcctctgg cgccactggg gctactgatg gcagcaccag
      601 tgctggcgct gctgatgctg ctgcggttgc ccggcacacc accacagccc acaccggagt
      661 gcaagagcgt cagtctgaag cgggattgtc tgccttatct gctttgcgca atattactgg
      721 ctgcggcggt gagcatgatg cagcttggac tttcgcctgc ccttactcgc cagttcgtca
      781 cggataccac cgccattagc caacaggtgg cctggctgtt gggtctgtcc gcagtagctg
      841 cgcttatcgc gcagttcggg gtactccgtc cgcagcgcct gactccggtg gccctgctcc
      901 tgagtgccgg agtgttgatg agtggtggtc tggctatcat gctctccgaa cagctatggt
      961 tgttttaccc gggctgtgca gtgctgtcat ttggagcagc tctggcaaca cccgcttatc
     1021 aacttctact gaatgataag ctggctgatg gcgcaggcgc gggctggctc gctaccagtc
     1081 acacacttgg ctatggactg tgcgctttgc tggtgccgct ggtatcgaaa acaggtgtcg
     1141 caatcgcgct gattatggca gcattatttg caactatatt atttaccatt gtgtctgtat
     1201 ttatctggca ttaccgtact atcaaataaa gatatgatca tcaccttact ggagtaaatc
     1261 agaggatatg agagaggacc tgcgtctgat gaaagcatgt caggcagtgt tctggcttgc
     1321 tgcccccgcc catcaggtat agtgaattca accagttgct gcggtgtaat ctccatgatt
     1381 ctttcgtgca acaacctatg atcccgttta ccattaatat tcagcgttgc tctgttatta
     1441 aatttcattc tgacaagcca gtcagctttt attccgggag tctgctctgc gcgttcaaac
     1501 aggtcataaa aatcgccttc acgatcagaa attgacaata catgggtatc agggcattgg
     1561 cgagccagtt ctgttgcttt attatatcct tcaacccaac gccaggtttc tttttcactg
     1621 acatgttttt tatgaagtaa atcattgcag atttcttgtc gggtattttt actggttttt
     1681 aattcatccc tgaaccactg atagtcatca taaacaccga ggcaaacacc tgactcactg
     1741 atgacgagtt gaggatgaag aaataagttt ctttcatctt tatgtttttt gtgaccaact
     1801 ccttgttttt ctttctgacc agaataatta agttctgttg tatcctgtaa aactaaaact
     1861 cttttaaact gctgagctct ttcagtgata ttttctttgt gaggagccat aattttatta
     1921 gcagagactt tatcgtttga aaaacaacga taagctgcct ttgtttcact ccaggtacgg
     1981 caggaaacag gaatactttc ttctggtgaa cgactgaggc tattgaggat atgttttact
     2041 cttttatcaa gtcttgagtc ccctatatcc agtgatttta gctcctgaat aacccagttt
     2101 tccataacac ctctgaaata atctctgtct tttcaatata atactattat ttcaacttgt
     2161 g
//
LOCUS       DAZOZD010000415         2155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOZD010000415 DAZOZD010000000
VERSION     DAZOZD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.421"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            367..1398
                     /gene="repA"
                     /locus_tag="ACHUPB_004512"
     CDS             367..1398
                     /gene="repA"
                     /locus_tag="ACHUPB_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR0761115.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     LTNRRRGEHSTKPKCENPSWYRPERYKALKGQLGHAYNRLVKKDPVTGEQSLRMRMSR
                     HPFYVQKRTFVGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVTRLAEEISPKD
                     SEGHVIPELEVTVSRLSRLLAEQVRFGVLGVSEETMWDREHRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKKR
                     REKAAASKRANRLKKLPVDQQIYEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT          582 a          585 c          561 g          427 t
ORIGIN      
        1 ggcactggaa gagaagagat cgttgtaatg cttttcaaat taacgtaaag cgggtatatt
       61 tcggctgtta ttagctgtgc agagggtggc actctgtgga gcaaagcggc gaaagccgga
      121 cggcagaatg cgccataagg cattcaggag agatggcatg tacgggcagt aagtcagaag
      181 actgaagatg ttccggaagc cataaaagga aaacccccac tatctttctt acgaacttgg
      241 cggaacgacg aaagatagtg ggggcctcac agaatacggg taaagtataa tgaaaccgta
      301 ccagagattc aaccctgtgc agtgtataaa tacacggcac aatcgctccg ccataagcga
      361 cagcttgtgg caggtctgaa gaatactcca tataacgcag tacactggag tcagttagca
      421 cccgaagagc agatccgttt ctgggaagac tatgaagcgg gaagggcgac cactttcctg
      481 gttgaaccgg aaaggaagct cacaaatcgc cgtcgtggtg agcactccac taaacccaaa
      541 tgcgaaaatc cgtcctggta tcgtcctgag cgctataaag cgctgaaagg gcagctcggg
      601 cacgcctata accgtctggt gaaaaaggac cctgtaaccg gcgagcagag cctgcgcatg
      661 cgcatgtcgc ggcatccttt ttatgtacag aaacggacgt tcgtcggtcg caaatacgct
      721 ttccggccgg aaaaacaacg tctcctcgat gccatctggc cggttctggt cagcttcagt
      781 gatgccggca cacataccgt ggggatgagc gtcactcggc tggctgaaga aatcagcccg
      841 aaagacagcg agggacatgt tattccggaa ctggaagtga cggtctcccg tctttcccgt
      901 ctgctggccg aacaggtgcg ctttggtgtg ctgggtgtgt cagaggaaac aatgtgggac
      961 cgtgaacatc gtcagcgtct gccacgttac gtctggataa caccggcagg ctggcagatg
     1021 ctgggcgtcg acatggtaaa acttcacgaa cagcagcaga aacgactgcg tgaaagtgaa
     1081 atccgccagc agctcattcg ggaaggtgtt ctgcgtgagg atgaagatat ctccgttcat
     1141 gcggccagaa aacgctggta tctgcagcgc agccaggatg ccctgaagaa gcgccgtgaa
     1201 aaagcggcag ccagcaagcg tgccaaccgc ctgaagaaat tacctgttga ccagcagatt
     1261 tatgagatgg ctgagtatct ccggaagcgt ctgccgccgg atgaagccta tttctgttcc
     1321 gatgaccatc tgaagcgact ggccatcagg gagttgcgtc agcttgaact gacgctggct
     1381 gccccgccac cgcactagac agcatcattc cctcagcact gaatcatcgc cagcccctcc
     1441 ggggctttcg gcgcagattc cgctcagccc aaaatcctta gtagtcacct taaatcccct
     1501 cagaggggca tatctgccca taaaaccacg catcagtcat cagaacgtgg ccacgttgtt
     1561 tcagttatcc acataaatcc gcaaacaaag aactttaaga agctgcaaac ctgaaacatc
     1621 aaacctgcaa tatattctta accccatcat ttaatcccct gcgttgcttc gccgcaggga
     1681 aagtctttat ctctgagacc actgtgaaca aatacaaaaa ggccttcgct tgcagcggcc
     1741 aaggccgcgc cgctcagaat ttaaaagtac ctcccacgct aacgcgcggg ccccgaacct
     1801 caccattctg aaaccacagc aaaaagacac caggaataaa aacaccacac aagcgcagca
     1861 tcctgctcac ccctcataac tgaaaagcga ggccgccccc gcccaaaggg cgggaacaac
     1921 atcgctttta attatgaatg ttgtaactac acagcatcat cgctgccagt cttctggctg
     1981 gaagtcctcc gtacacgctc gtaagcggcc ctgacggccc gctaacgcgg agatacgccc
     2041 cgacttcggg taaaccctcg tcgggaccac tccgaccgcg cacagaaact ctctcatggc
     2101 tgaaagcggg tatggcttag cagggctggg gatgggtaag gtgaaatcta tcaat
//
LOCUS       DAZOZD010000416         2154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOZD010000416 DAZOZD010000000
VERSION     DAZOZD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.422"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            113..784
                     /gene="hprR"
                     /locus_tag="ACHUPB_004513"
     CDS             113..784
                     /gene="hprR"
                     /locus_tag="ACHUPB_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0761116.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            784..2142
                     /gene="hprS"
                     /locus_tag="ACHUPB_004514"
     CDS             784..2142
                     /gene="hprS"
                     /locus_tag="ACHUPB_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0761117.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
BASE COUNT          647 a          387 c          492 g          628 t
ORIGIN      
        1 aatgagtttt gaggatagcg aaaaagcggc cagggtaacc ttacagcagc attacaattt
       61 tgtaatgaac taggctgttt ctataacata tgatttatgg catattattt tcatgaagat
      121 tttacttatt gaagataatc aaaggaccca ggagtgggta acgcaggggc tttccgaagc
      181 gggttatgtc attgatgccg tttctgatgg cagagatggg ctttatcttg cgctgaagga
      241 tgattatgca ttgatcattc tggatattat gcttccgggt atggatggct ggcagatctt
      301 acaaacgtta agaacagcaa agcaaacccc tgttatttgc cttactgcaa gggattctgt
      361 cgatgacaga gtcagagggc tggacagtgg agcaaatgat tatctggtaa aacctttttc
      421 attttctgag ttgctggcaa gggttcgggc acaattaagg caacatcacg ctttgaattc
      481 aacattagaa atcagtggct taagaatgga ctctgtaagt caaagtgtga gcaggaacaa
      541 tatcagtatt acactgacgc gcaaggagtt tcagttactt tggctactgg cctccagagc
      601 tggcgaaatt atacccagaa cggttattgc gagtgaaatt tggggaatca actttgatag
      661 tgataccaat acggtggatg tcgccattcg caggctccgc gcaaaagttg atgatccttt
      721 tcctgaaaag ctaatcgcca caatccgggg gatgggctat tcattcgtag cggtaaaaaa
      781 ataatgaaaa gattatctat aaccgtccgt ttaaccttgc tttttatatt gctgctgtct
      841 gttgctggcg ctggaattgt ctggactctc tataatggcc tggcaagtga gttgaaatgg
      901 cgcgatgata caacactcat taaccggaca gcacagatca agcagttgtt aattgatggg
      961 gtaaatccag atacgttacc tgtatacttt aaccggatga tggatgttag tcaggatatc
     1021 ttgatcattc atggtgatgg catcaataaa attgttaacc ggacaaatgt cagtgatgac
     1081 atgttaaata acatacctgc tagtgagaca atcagcgcag ctggcattta cagaagcatt
     1141 attaatgata ctgagattga tgctttacga attaatattg atgaagtttc gccatcatta
     1201 acggttactg tggctaaatt ggcttcagcc agacataaca tgcttgaaca gtataaaatt
     1261 aatagcatta taatttgcat tgtcgccatt gtactttgct cagtattaag tccgctgtta
     1321 atcagaacgg gattacgaga gatcaaaaag ttgagtggtg taacggaagc gctgaattat
     1381 aacgatagcc gggagcctgt tgaggttagc gcattaccga gagaactaaa acctcttggg
     1441 caggcgttga ataaaatgca tcatgcttta gtcaaagatt ttgagcgtct aagtcaattt
     1501 gctgacgatc tcgctcatga acttagaacg cccattaatg cattactggg tcagaatcag
     1561 gttacgctca gtcaaaccag aagtatcgct gaatatcaaa aaacaattgc cggtaacatt
     1621 gaagagctgg aaaatatttc gcggttaaca gagaacatac tgtttcttgc ccgggcagat
     1681 aaaaacaatg ttttggtgaa actggactcg ctttctctca ataaggaagt cgaaaatttg
     1741 ttggattatc ttgaatacct ttcagacgag aaagagattt gctttaaggt cgagtgcaat
     1801 cagcaaatct ttgcggataa aattttactg caacgaatgt tatcgaatct tattgttaat
     1861 gccattagat attcaccaga aaaatcgcgt attcatataa ccagttttct tgataccaac
     1921 ggctatctta atattgatgt cgccagtcct ggaacgaaaa ttcatgagcc tgaaaaactc
     1981 ttccgtagat tttggcgggg agataattcg cgtcattccg taggtcaggg acttggtctt
     2041 tctttagtca aagcgattgc cgaattacat gggggaagtg ctacgtatca ctatctcaat
     2101 aagcataatg tgttccggat tatgttaccg caaagaaatt aacatagggc ttca
//
LOCUS       DAZOZD010000417         2150 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZOZD010000417 DAZOZD010000000
VERSION     DAZOZD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.423"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(113..583)
                     /locus_tag="ACHUPB_004515"
                     /pseudo
     CDS             complement(113..583)
                     /locus_tag="ACHUPB_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            919..1104
                     /locus_tag="ACHUPB_004516"
                     /pseudo
     CDS             919..1104
                     /locus_tag="ACHUPB_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1204..1410)
                     /locus_tag="ACHUPB_004517"
                     /pseudo
     CDS             complement(1204..1410)
                     /locus_tag="ACHUPB_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(1463..1714)
                     /locus_tag="ACHUPB_004518"
     CDS             complement(1463..1714)
                     /locus_tag="ACHUPB_004518"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761118.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          595 a          541 c          497 g          517 t
ORIGIN      
        1 aatatccctc tgatatgaca acaaaaaacg gagccggatt cctgtttttg tgaactgcag
       61 ctattcgagt ctaaaaagtg atccacttac caccaacggc ctaatatata cagaacggag
      121 taaacagggc taacagttcc cgacaggtct catcattccg tggcccgaaa gtgtaagcca
      181 acacaccgcc agttttagtg ttgtacgcat accagagcca gtgttgttgc agccttgctg
      241 ccaacgaagc gccactgctc atcaagttca cagataaggg caacatcagc gtgagcgact
      301 ggcgcagatg ttatccgccg gggcgggagc tttttaatgt ccgtatgacg gtattgatgc
      361 cgattttcag tgtcctggcg gtatcacggc cccctgcgcc gttatgtgcc atttcaacga
      421 tctgctcctt aacgccgggt tttctggctt cataagagta agtgagctgg aaaacacgtt
      481 tacatgagcg acaacggaaa cgctcatgcc gggaacaact acggccatgt cgataaactt
      541 catcggaatg acagcgcgga caatgaacag taacggtagc catggaaaac cttaaatacc
      601 agtagattac cagaaacttc tgtcgattga agacatcacc cgaagtttga attcattcga
      661 atacttttta cgaggttctt ttcgccagtc ctgtaattcc atacttaggt gttcgtctat
      721 gtcagatggg cgtctaagtt accaattctt gtctaatggc tacatacggt ggtcggttta
      781 cgcttaccgc aggacaggtg gtaactgaaa aatagtttgc tgaggcgact ggaaacagaa
      841 gggcaagatc gattccgaca gcagtgatgg tcagctacct catcgataac tttgcaacag
      901 tgcccttctt gaagcatcat gcttcatgaa gaaaaactgg cacgtcatca acgtaaacag
      961 gcgatgtata cccgaatggt agccttcccg gcggtgaaaa tgttcgaaga gtatgacttc
     1021 acgttcgcca ccggagcacc gcagaagcag ctccagtcgt tacgctcact cagcttcata
     1081 gagcgcaatg aaaacatagt gttacagggg acatctgaca tcacaaatcc ccgggtgggg
     1141 atttgtgtat aagagacagc ccatggagga aggggatata tcgcagctct gacgactcac
     1201 tccttattta cccgggaaat acttctccag caatgcatac aggatggcag cggtttcagc
     1261 atccattatg ccgtcataat ttttctgccg gaaatgaagc tgaaaagcgc ggacaagcgc
     1321 cttatatccg tcatccgtct ccacaccaga aatactgtat ccataggtct tcagccgtga
     1381 cacgatatca accttatccg gaatgccctg cgattcaaaa aataaatatt tagtcagtaa
     1441 ttttatgttt ttcctgaaaa aattactctt tttcgaactc cagtgagcgt tcacgactca
     1501 tcagtgtgaa aatgatggca aaaacagcag acagtatcag tacgataaat gtggcccccc
     1561 atcccagata atcgacaaca agtcccattc ccagtgtcgc aacaattgag ccgaagatat
     1621 aggaaaatga gcctgacagc cctaacagaa taagtttacg cgtaaatcgc ccggaatcca
     1681 gaagagcatt aagtatcaca taaacaggtt ccataccagt cctgaccgca tcaggacgcc
     1741 agtaaataaa atctcgtagt ccaccggcat cagagacaag agtaatcgtc gttaccggta
     1801 tttcgactgg tttgtactgg tgtgagcgga caaaactact gtacgtctct catacacgct
     1861 cgtaagcggc cctcacggcc cgctaacgcg gagatacgcc ccgactgcgg gtaaaccctt
     1921 gtcgggacca ctccgaccgc gcacagaggc attttcatcg ctgacaggcc gcatggcctc
     1981 gcagagccgg agggggactg gtgatattcg gacgaccgta ccggcttgcg ccgggcttcg
     2041 gcggttttac acctgtgcta catgtaacca ccgtatgccg ccttatatgc gctgactcgc
     2101 attatttgcc ttgttccgtc tgaaagtaat cactatgatt aaatatgatt
//
LOCUS       DAZOZD010000418         2139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOZD010000418 DAZOZD010000000
VERSION     DAZOZD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.424"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUPB_004519"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUPB_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0761119.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUPB_004520"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUPB_004520"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR0761120.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
BASE COUNT          598 a          501 c          464 g          576 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctga
//
LOCUS       DAZOZD010000419         2137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOZD010000419 DAZOZD010000000
VERSION     DAZOZD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.425"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            214..1191
                     /gene="intI2"
                     /locus_tag="ACHUPB_004521"
                     /pseudo
     CDS             214..1191
                     /gene="intI2"
                     /locus_tag="ACHUPB_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            complement(1379..1618)
                     /locus_tag="ACHUPB_004522"
     CDS             complement(1379..1618)
                     /locus_tag="ACHUPB_004522"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR0761121.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          618 a          467 c          445 g          607 t
ORIGIN      
        1 acactctgtt attacaaatc gtgcatgccg tctatcctgc ttgcacgatg cacattatct
       61 cacccaaaga actcataaca ttttaattta tttagcatta aattaaaact ctaccggtaa
      121 acaagcatct ctaggcgtaa aaaacaggca cttttttaaa tcccagctag aataggctgt
      181 ataggcagac agttgcaaga caaggagcgt tttatgtcta acagtccatt tttaaattct
      241 atacgcacgg atatgcgaca aaaaggttat gcgctgaaaa ctgaaaaaac ttacctgcac
      301 tggattaagc gttttattct gtttcacaaa aaacgtcatc ctcagaccat gggcagtgaa
      361 gaggtcaggc tgtttttatc cagcttagca aacagcagac atgtagccat aaacacgcag
      421 aaaatcgctt taaatgccct agcttttttg tacaacaggt ttttacaaca gccgttgggc
      481 gatattgatt atatccctgc aagcaagcct agacggctac cctctgttat ctctgcaaat
      541 gaagtgcaac gcattttgca ggttatggat actcgcaacc aagttatttt tacgctgctg
      601 tatggtgcag gtttgcgcat taatgaatgc ttgcgtttgc gggttaaaga ttttgatttt
      661 gataatggct gcatcactgt gcatgacggt aagggtggga aaagcagaaa cagcctactg
      721 cccacgcgcc taatcccagc aataaaataa ctcattgagc aagcgcggct tattcagcaa
      781 gacgacaact tacaaggcgt agggccatcg ctgccttttg ctttagatca caaataccct
      841 tctgcttatc gacaagcggc gtggatgttt gtctttccct ccagcacgct ctgcaaccac
      901 ccgtataacg gcaaattatg ccgccatcat ctgcatgact ccgttgcgcg aaaggcattg
      961 aaggcagccg tacaaaaagc aggcatcgtt agcaagcgtg tcacttgtca tacatttcgt
     1021 cactcgtttg ctacgcatct attacaagcg gggcgtgata ttcgcactgt gcaagaactc
     1081 ttagggcata acgatgttaa gaccacgcaa atctatacgc atgtgttggg tcagcatttt
     1141 gccggcacca ccagtcctgc ggatggactg atgctactta tcaatcagta aaagtagata
     1201 aatatcttta tgtagccctg agtagcccct gatacttttt atggtgaaaa agataaacaa
     1261 tcatagaggg gataaatatg agccagttct accattgttc agataagttt aagcaagata
     1321 ctgtcaatca cagtgaccat tcatggtcat tacacttcct ccagttgcca gacaatgcct
     1381 aacataaaaa cgcaaaagcc caaaagtcag agcttaggtg ttctaggcgt tttctaagcg
     1441 ctgaagaaat acgtcttacc gtagattgcg tgcgtttctg aagcacattt tccttataca
     1501 tggctttatc ccaggcagct tcatcaagct cttggagtaa caagccagta ataatgcgac
     1561 actctcgggt ttgtagcgag ccgctggtga gatcggagtt atagcggaaa ggggtcattt
     1621 gtaattattt gtgggtggag gatagttaac ttattattgt agttggaaga ggctgctttc
     1681 caaacattag cacctcgtaa tgcttgttac tgtatattaa tttatgctct tagcgcatgt
     1741 taatgggcta ttacaatgca taatgtccac atcaagataa ctgtggctta acacctgtac
     1801 aagcgaaaaa aatcaatcac ctgtccggaa atacgggatc actgtaaagt gttggtgagg
     1861 cagtctagga gggggattta atttgggata ctgatgaaag gattatgatc agttggatat
     1921 aggatcgtac taagctgagc ggcaggttag ctagattagt taaccagata agtgaaatct
     1981 agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct acacccagtt
     2041 cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc cacccctccc
     2101 agttcccaac tattttgtcc gcccacaggg ctgccga
//
LOCUS       DAZOZD010000420         2131 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOZD010000420 DAZOZD010000000
VERSION     DAZOZD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.426"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            101..610
                     /locus_tag="ACHUPB_004523"
     CDS             101..610
                     /locus_tag="ACHUPB_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0761122.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            607..2025
                     /gene="phrB"
                     /locus_tag="ACHUPB_004524"
                     /pseudo
     CDS             607..2025
                     /gene="phrB"
                     /locus_tag="ACHUPB_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
BASE COUNT          514 a          530 c          569 g          518 t
ORIGIN      
        1 aatattttta ccaacaaaat acttccccgc aaaaactctg ccccataaaa ttcatttacc
       61 atcgtctaca cttactcctt tataccgttc aggagcagta atgaatctac aacgatttga
      121 tgacagcacc ctaatccgta tctttgccct tcatgagtta catcgactga aagaacatgg
      181 cttaacgcgc ggggcgcttc tcgattatca cagccgctat aaactcgtct ttctggcgca
      241 ttctcagccg gagtaccgca aacttggccc gttcgtggct gatattcacc agtggcaaaa
      301 tctggatgac tattacaacc agtaccgcca acgcgtagtt gttttgcttt ctcaccccgc
      361 caacccgcgc gatcacacca atgttttgat gcacgttcag ggttattttc gcccgcatat
      421 tgattccaca gaacgccagc agctggctgc gcttatcgac agttatcgcc gtggcgagca
      481 accacttctt gcgccgctga tgcgtatcaa acactatatg gcgctttatc atgacgcctg
      541 gctttcaggg cagcgttatt tcgaactttg gccgcgtgtg attaacttgc gccattcagg
      601 agttttatga ctacccatct ggtctggttt cgccaagatt tacgtctgca cgataatctc
      661 gcactggctg ccgcctgccg caattcgtct gcacgcgtgc tggcgttgta tatcgctaca
      721 ccacgccagt gggcgacgca taacatgtcg ccgcgtcagg ctgaacttat caatgctcaa
      781 ctgaatgggc tacaaatagc gcttgcggaa aaaggtattc ctttattgtt ccgtgaagtg
      841 gatgactttg tcgccagtgt cgaaatagtt aaacaggtgt gcgcggaaaa cagcgttacc
      901 cacctgtttt ataactatca gtatgaagtg aatgagcggg cgcgggatgt ggaagttgaa
      961 agagcgctgc gtaacgtggt gtgtgaagga tttgatgaca gcgtgatcct gccgcctggc
     1021 gcggtgatga ccggtaatca cgagatgtac aaagtcttta cgccttttaa gaatgcctgg
     1081 ctgaaacggc tgcgggaagg gatgccggag tgcgtcgctg cgccaaaagt tcgtagtagc
     1141 ggatcgatag agccctcgcc atccattacg ctgaattatc ctcgtcagtc tttcgatact
     1201 gcgcattttc cggtggaaga aaaagcggcg attgcgcaat tacgccagtt ttgccagaac
     1261 ggtgccggag aatatgagca acaacgagat tttccggcag tggaaggcac cagccgtttg
     1321 tcggccagcc tggcaacggg cgggttatcg cctcgccagt gcttgcatcg cttgttggct
     1381 gaacagccgc aggcgctgga cggtggggtc ggtagtgtct ggcttaatga gctgatctgg
     1441 cgcgagtttt accgtcacct gataacgtat cacccctcgt tgtgtaaaca tcgtccaatt
     1501 attgcctaga cggatcgtgt acagtggcag agcaatcccg cacatttaca ggcctggcag
     1561 gaaggcaaaa cgggataccc gattgttgat gccgctatgc gtcagcttaa cagcactggc
     1621 tggatgcata acaggctacg gatgattaca gccagttttc tggtgaaaga tttattgatc
     1681 gactggcgcg aaggcgagcg atatttcatg tcgcagctga ttgatggtga tttggcagcc
     1741 aataacggtg gctggcagtg ggccgcttca accggaaccg atgcagcgcc gtattttcgt
     1801 attttcaacc cgacaaccca gggcgagaaa tttgatctcg aaggtgagtt tatccgccag
     1861 tggctaccgg aactacgcaa tgtaccgggg aaatcggtgc atgagccgtg gaagtgggcg
     1921 cagaaagcag gtgtgaagct ggattatccg caaccgatag tcgagcacaa agaggcgaga
     1981 gtacaaacgt tggcagcgta tgaggcggcg cggaagggga aataacagac gcgtcaggca
     2041 atcgagccca gatgccggat gcggcgtgaa cgccttatcc agcctacaaa tttgtgcaaa
     2101 ttcaataaat tgcaggaaat acgtaggcct g
//
LOCUS       DAZOZD010000421         2099 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOZD010000421 DAZOZD010000000
VERSION     DAZOZD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.427"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHUPB_004525"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHUPB_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..>2099
                     /gene="fimH"
                     /locus_tag="ACHUPB_004526"
     CDS             1816..>2099
                     /gene="fimH"
                     /locus_tag="ACHUPB_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HFR0761123.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDN"
BASE COUNT          529 a          480 c          534 g          556 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataatta
//
LOCUS       DAZOZD010000422         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOZD010000422 DAZOZD010000000
VERSION     DAZOZD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.428"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUPB_004527"
     CDS             complement(60..1025)
                     /locus_tag="ACHUPB_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0761124.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUPB_004528"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUPB_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761125.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZOZD010000423         2086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOZD010000423 DAZOZD010000000
VERSION     DAZOZD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.429"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..854
                     /gene="espL1"
                     /locus_tag="ACHUPB_004529"
                     /pseudo
     CDS             1..854
                     /gene="espL1"
                     /locus_tag="ACHUPB_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1026..1133
                     /gene="yniD"
                     /locus_tag="ACHUPB_004530"
     CDS             1026..1133
                     /gene="yniD"
                     /locus_tag="ACHUPB_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0761126.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1186..1944)
                     /locus_tag="ACHUPB_004531"
     CDS             complement(1186..1944)
                     /locus_tag="ACHUPB_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0761127.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          646 a          463 c          422 g          555 t
ORIGIN      
        1 acgaatgcca tttgccccta tacatccatc ggcaatgttg ttaataatgg ccacaggcca
       61 gactcaatag cttattacac tattcaaaca gttgcccata ctccctgaaa aagaaatcat
      121 tgaaataata actgcgcaga atagcgttgg tacacctgct ttatttctgg ctatgatgaa
      181 cggacaaact gacaacgtaa aaatatttat gcaagaaatt cagtcactgg tatacaatca
      241 tatcattcat gaagataatc tggttaaatt actgcaaact aaaagtgcta acgaaacacc
      301 tggactctat atctccatgt tgtatggatt cgatgaaata atcgatatct ttctgaatgc
      361 attaaccact ccaatagcac aagaactttt aaacaaaaaa aatggtgatg agtattttag
      421 ccatgaaaat acatgatggt gagccaggat tatacgccgc aatggaaaat aatcaccctt
      481 tgtgtgtcac acggttcctc tctaaaatta atggcatcgc ctttaaatac aagttgagca
      541 aagctaacat catggattta ttaaaaggag ctacagcaca gggaacccct gcattataca
      601 tcgccatgag caagggtaat gaagacgtcg tgttatctta tatatcgacg ctgggtgctt
      661 ttgcaaaaaa acattctttt agtcaacatc agttatttac actattggcc gctaaaaatc
      721 atgacaacat gtcagctgtt catatagcca ttcatcataa gcattataaa actgtagaaa
      781 catattatgc tgctataaat gtcatcagcc aaagcctgag ttttagtgct gatgaaataa
      841 agacgtattt ataaccagct atttacgata tatatcgata cataatttta tttttttcat
      901 aaaacgcaga aacagtgacc atgtgcacaa cgccacccga cggctgtaat aacaaaaaat
      961 cccttgatgc ctatcccttt tgttacactc cgttatcacg cacaagagat atgcaggaca
     1021 ctggtatgcc gactaaacgc tttgataaaa aacactggaa gatggtggtg gtgctactgg
     1081 caatctgtgg cgctatgttg ttgctacgtt gggcagcaat gatttggggc tgagcagtga
     1141 caaacgaaga cagccagacc gcataacacg gtccggcaga taattttaca tgctgtagcc
     1201 cagcgataag gttgtccgac gatcggtatg ttccggtgca gactctggag gttcagagtt
     1261 ccatgtgacg ttgtaagcca ccttcagtcc aaaatgttca ttaatagcaa catttaatgc
     1321 gctttcggag ttcagcgttg tatcttccgc gccaaagacc gaaacaccct gcgtaaattt
     1381 agcgttgtca gtcaactgcc atgcataagc cccggaggca tagcccagcg gctgggtttc
     1441 actggcattg tcggtatatt tgtcgtaacg cacacctggg ccgaattcaa agcggaaact
     1501 gtgtaccggg ccattcaaaa actggcgacc ataacccgcg gtcaacacat cgcgctctcg
     1561 atagccgtta taacggtctg tcagccagct tgcctgacca aataaatagt cataatcagt
     1621 taaattaaaa cggctacgcc cgcccgccgc atatttttct gaagaacgct catcattaga
     1681 agaagtatta ctggcgttcc cccacagcga ccaggcagtg gtttgtccat accaggtcat
     1741 ggtggtgtca gccgtaagtg aggagctttt cgtattgcct gattgtgcaa gatatcctgc
     1801 gttcagatta ccttcgaaag gttttttagc gctggcagga tcgtccatga cagtaaaaac
     1861 ggaatcatcg gcagctgcat tcagtgacgc aaacatgccc cccgccaaca taacgatggc
     1921 aggaactgtc ttcaaaagct tcatttatca agagtccgta caacaaaaaa agagaccatc
     1981 gcggtcccgg aaactttctt aaggatcaaa gattagcgtc cctggaaagg taacgaatta
     2041 taaaaaggcg cgaataactt agcaatgtat tcttatttca tttttt
//
LOCUS       DAZOZD010000424         2073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOZD010000424 DAZOZD010000000
VERSION     DAZOZD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.430"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            2..698
                     /locus_tag="ACHUPB_004532"
                     /pseudo
     CDS             2..698
                     /locus_tag="ACHUPB_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1451
                     /locus_tag="ACHUPB_004533"
     CDS             705..1451
                     /locus_tag="ACHUPB_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0761128.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1466..1888
                     /locus_tag="ACHUPB_004534"
     CDS             1466..1888
                     /locus_tag="ACHUPB_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0761129.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1889..>2073
                     /locus_tag="ACHUPB_004535"
     CDS             1889..>2073
                     /locus_tag="ACHUPB_004535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761130.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          568 a          456 c          604 g          445 t
ORIGIN      
        1 tattcgcttc tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc
       61 tcgcggcgtt acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc
      121 gttaattcag gttggatggc tggtcgaaaa tgacggaggg ctttctctac ctaactttga
      181 acgtcataac ggaaaaagct ctaaaaaaaa cgggcggtta caaacgagcg agtaacaaaa
      241 atacgcgaac tgaaacgaaa aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg
      301 ttaccagagg aagaggaaga ggaagaggaa gaggaagagg aagaggaaga tctaaatact
      361 gatctccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct attgagctgc
      421 ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag gcattgcgaa
      481 aaccgattcg aacggagcag ggcgctaacg gggcgatacg ggaactggaa aaattccgtc
      541 agcagggttt tacacctgag caggtgattc gacacagcat cgccaatgaa taccagggcc
      601 tgttcgcgcc gaaaggtgtt cggcctgaga cgttgctccg acaggttaac accgtctcgt
      661 tgccggacag tgcgatcccg ccaggcttca gggggtaacg gaccatgaaa aatattgcga
      721 caggcggcgt tctggaacgc atccgccgac tgaccccgcc acatgtaacc gccccattca
      781 gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt agcgaggaga
      841 tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct gaatcgctca ggcatccagc
      901 cgttgcaccg taaatgctcg tttgcgaatt accaggtgca gaacgacggc cagcgatacg
      961 cgttaagcca ggcgaaatcc atcgccgatg aactgatgac cgggtgtaca aattttgcgt
     1021 tcagcggaaa acctggtacc gggaagaatc acttagcggc agctatcggg aatcgcctgc
     1081 tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagc gccctgcacg
     1141 ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggagctgtgc gaagtggatc
     1201 tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag gtggtgctgc
     1261 accagattgt tgatcgccgg acagcgtcga tgcgcagcgt ggggatgctg acaaacctga
     1321 actatgaggt gatgaaaaca ttgctcggcg agcgggtgat ggatcgcatg gtcatgaacg
     1381 gcgggcgctg ggtgaatttt aactgggaga gctggcgtcc gaatgttagc cattcgaggg
     1441 ttgttaagta gtttcaggag gatttatggc gaaacctttt actcccgaac agcgggaaga
     1501 actgaagacg cgaattgtgg aactcgtgca tcaggacggt cgggtcacga ttcggcagtt
     1561 gtcagatgaa acaggtatca gtcgtgcgtc tgtcggtcgc ttatgcatag aactggtcgc
     1621 aagtggtgat gtatataatt ctggctacgg cttattcccg tctgaacagg ctcgcaagga
     1681 ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg aggaaaccgg ttgttgttga
     1741 tccggacctt atctggtcat tacctgacgg agaaatacgc cgctacgaca ggcgcctgaa
     1801 tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
     1861 gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc aattaccgct
     1921 ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt taagcaatat
     1981 cgttttattt ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc
     2041 atcggaaaga ccatattgcc atcacgcctc tgt
//
LOCUS       DAZOZD010000425         2047 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOZD010000425 DAZOZD010000000
VERSION     DAZOZD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.431"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            45..1544
                     /gene="pitB"
                     /locus_tag="ACHUPB_004536"
     CDS             45..1544
                     /gene="pitB"
                     /locus_tag="ACHUPB_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0761131.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1593..>2047)
                     /locus_tag="ACHUPB_004537"
     CDS             complement(1593..>2047)
                     /locus_tag="ACHUPB_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFR0761132.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          470 a          506 c          551 g          520 t
ORIGIN      
        1 gataatgcgt ccgttcgtaa attcaaaatg gcgtaatcta atatatgcta aatttatttg
       61 ttggccttga tatatacaca gggcttttgt tattgcttgc tctggcattt gtgttgttct
      121 acgaagcaat caatggtttt catgacacgg cgaatgcggt ggcaaccgtt atttatactc
      181 gtgccatgca accacaactt gctgtggtga tggcggcatt ttttaacttt tttggcgtgt
      241 tattgggcgg acttagcgtt gcctatgcca ttgtccatat gttgccaacc gatttgttgc
      301 tgaatatggg gtcaacccac ggcctggcga tggtcttttc catgctgctg gcggcgatta
      361 tctggaacct gggaacgtgg ttcttcggtt taccggcctc cagttcgcac accttgattg
      421 gtgcgattat cggcatcggt ttaaccaacg cgctgttaac cggctcatcg gtgatggatg
      481 cgttaaacct gcgtgaagtg accaaaattt tctcctcgct gattgtttcc cctatcgtcg
      541 gcctggtcat tgcgggaggc ctgatattcc tgctgcgacg ctactggagc gggacgaaaa
      601 agcgtgaccg tattcaccgc attccggaag atcgcaaaaa gaaaaaaggc aaacgtaaac
      661 cgccattctg gacgcgtatt gcgctgattg tttccgctgc gggcgtggcg ttttcgcacg
      721 gcgcgaacga cggacaaaaa gggatcggcc tggtgatgct ggtactggtg gggattgccc
      781 ctgctggctt cgtcgtcaat atgaacgcgt ccggctatga aattacccgt acccgcgatg
      841 ccgtcaccaa cttcgaacac tacctgcaac agcatcctga actgccgcag aagttgattg
      901 cgatggaacc tccattgcct gcagcatcga ctgatggcac gcaagtaaca gagtttcact
      961 gtcatccggc aaataccttt gatgcgattg cgcgcgttaa aacgatgctg ccaggcaata
     1021 tggaaagtta cgagccgtta agcgtgagtc agcgcagcca gctgcgccgc attatgctgt
     1081 gcatctctga tacctccgcg aagctggcga aactgccagg cgtcagtaaa gaagaccaga
     1141 acctgctgaa aaaactgcgc agcgatatgt taagcaccat tgagtacgct ccggtgtgga
     1201 tcatcatggc ggtagcactg gcgctcggca ttggcaccat gattggctgg cgtcgtgtag
     1261 cgatgaccat cggtgagaag attggtaagc gcggcatgac gtatgcgcaa ggcatggcgg
     1321 cacaaatgac ggcggcagtg tctatcggcc ttgccagtta tattgggatg cctgtctcca
     1381 caacacacgt cctctcgtct gcggtagcag ggacgatggt ggtggacggc ggtgggttac
     1441 agcgtaaaac ggtaaccagc atcctgatgg cgtgggtgtt tactttaccg gcggcaattt
     1501 ttctttctgg tgggctgtac tggatagcat tgcagttgat ttaaccttcc tgaaaatgcc
     1561 cggtcctggc gatcgggcat ttccattttt gactagtgat aaccacgcgc ggtcataaaa
     1621 tccgtaatcg ctttttctgc atcaaccaac acctgttcca gtggctgatt ggcatcgata
     1681 tcaaccagtt gtgcaccacc aaaggttaac tgtggcgtta tggcaatctt cctcgccagc
     1741 gattcccgtt tatggtcggg tttacgtgca caggcaactt caaggtcaac attgagtttg
     1801 atgaccagat caggcttatg gctcgccatc cagtgaaacg ctttacgttc ctggcttgcc
     1861 agccatgaga caaaacgacc accttcaacg ttaggtggga acaccgtacc atcgtaagcg
     1921 ccaggaattt ggtcctgagg ataacggtcg gttagaacaa ttaacccgcg acgacgacag
     1981 gcaagcatat gacgaaagcg cagtaagcga cgggcgacaa acgctgtaat taccagcgcc
     2041 ggaactg
//
LOCUS       DAZOZD010000426         2045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOZD010000426 DAZOZD010000000
VERSION     DAZOZD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.432"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHUPB_004538"
     CDS             <1..326
                     /locus_tag="ACHUPB_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0761133.1"
                     /translation="VITMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS
                     YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMK
                     DSQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="ACHUPB_004539"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="ACHUPB_004539"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0761134.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          497 a          504 c          482 g          562 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttattatcg tcttaatgat ttccacatat taaaagcaag tatgctttca
     2041 aaaca
//
LOCUS       DAZOZD010000427         2024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOZD010000427 DAZOZD010000000
VERSION     DAZOZD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.433"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..305)
                     /locus_tag="ACHUPB_004540"
     CDS             complement(<1..305)
                     /locus_tag="ACHUPB_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0761135.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
     gene            complement(344..631)
                     /locus_tag="ACHUPB_004541"
     CDS             complement(344..631)
                     /locus_tag="ACHUPB_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761136.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(881..1036)
                     /gene="hokD"
                     /locus_tag="ACHUPB_004542"
     CDS             complement(881..1036)
                     /gene="hokD"
                     /locus_tag="ACHUPB_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0761137.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1264..1929)
                     /locus_tag="ACHUPB_004543"
     CDS             complement(1264..1929)
                     /locus_tag="ACHUPB_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0761138.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          516 a          524 c          459 g          525 t
ORIGIN      
        1 tgctctgcgg cttcgctcag agctggcgta tgttcccggc ctactgattc acacaggtaa
       61 tcaacgaaac cggacaccag ttttcgttcg cgaggcgtga tcgccccacc gaccggagtc
      121 cagtaatcga aacccagttg caggagtttg aaaaaacgct tgtggaatgc gtagttacac
      181 acacgcttaa agtctgcgtg tatccactca cctattttga tttgatgcag aaaatcgcaa
      241 ctctccggcg tcgccgggag aagtaatccg gaagaggttt gtttgaccag ttgtatatgt
      301 gccatcggct ttctccggtg gcacggtgtt acacagcagg agttcaatcc tgctcaagat
      361 tgtagatgag tttattcttc agcaaaagca gaaaaccagc cttaaaacca atctctttca
      421 aaacccgtaa tgatgtgaca aattcgtcct cacgcaaaat aaaaccgtcc gtcagaagtc
      481 cattacaaaa ataaaataac acagcaccgc tcttcctttg ttgagattgc aaacatctaa
      541 tgcggcaatg gctgacaatc gctccattct caacgcgcac agcatagagg ccattttcac
      601 taaaaatttc acgcaattct tcgattttca tcttcagaat ccttccagat aaatagctct
      661 cccctgttcg gggtccatcc ctcttctccc tgcgcgctac ttaagtgagt cgattctatc
      721 tgcgaaggtg cgcgaatcaa attcaccgga aataaacaac aaaaaacccg ccgaaacgct
      781 gagagatcac agcgatgggc gggttaagtg cgggtgcgtt gaggatgcct gacacatcag
      841 aggtgggcgg ggatgggatc agctccccgc ccggtcactc ttacttcctg gattcgtagt
      901 ctacgaagac agcgacctcc gtctggccgg ttcggattcg tacctcacag aggtctttcc
      961 tcgttaccag tgccgtcact atgacggtta aacagatgac gatcagggcg attaacatcg
     1021 ccttttgctg cttcatagcc tgcttctcct tgcctttcgg catgtaagag gctaacctac
     1081 atgtgcaaag catgaaattg gcctcagatt aatgttaagc gtcttgccgg acgcgtaatg
     1141 ttaactgggg cttttctcta tctgcctttt ggtgttcatg cctgaagcag atagcctcaa
     1201 gcacccgcag tcattctact tacctcgcca atatgaaatc aatcagaaag gtgccccata
     1261 aaatcactcc ttctcttctt taccgtagtg gagttgacca attttgataa gagggcgtcc
     1321 ctgagatttg cggtgtagat tggtatcgcg cagagaatac acacagccac aatattcctg
     1381 ctgatagaat ttttcgcgct tgctgatttc aatcatacgg gacgagccgc cctgcttgcg
     1441 ccagttataa tcccagtaca ccatgcccgg ataatgcgcg acggctcgcc gcccacagtc
     1501 gttaacctgc tgcatatttt tccagcgtga aatgcccagt gaactgctga tcacactgaa
     1561 accattttca gcggcgtaca gcgctgtccg ctcaaaacgc atgtcaaaac acatggtgca
     1621 acggatccct cgttcgggct cccattccat tcctttggct cgttcaaacc agttgtctgt
     1681 gtcgtaatca gcatcaataa acggcacgcc gtgttgttca gcaaagcgaa tattctcatc
     1741 cttacgaatt aaatactctt tctgaggatg aatgttcggg ttgtagaaaa agatggtgta
     1801 gtcgattccc gaggcctgaa gcgcctccat cacctcaccg gaacatggag cacagcaaga
     1861 gtgcagtagt agtttgtttg ccccgtttgg gagctccaat ttgggccgtt tgaaatcagc
     1921 aactgtcata aatatgttta ttggggtcat aaaaatatca aagagtgtag cattagaacg
     1981 gggctatcgg aaacagatgt gtgactgctc cccgcccttt cggg
//
LOCUS       DAZOZD010000428         2018 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOZD010000428 DAZOZD010000000
VERSION     DAZOZD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.434"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..702)
                     /gene="ipaJ"
                     /locus_tag="ACHUPB_004544"
     CDS             complement(<1..702)
                     /gene="ipaJ"
                     /locus_tag="ACHUPB_004544"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR0761139.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELG"
     gene            complement(899..1708)
                     /locus_tag="ACHUPB_004545"
                     /pseudo
     CDS             complement(899..1708)
                     /locus_tag="ACHUPB_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          582 a          519 c          331 g          586 t
ORIGIN      
        1 acctaattcc atagttgaaa aacaagaata attttctcct acagctggat ccatatagct
       61 tccatcaggt ctacacaaca cccagtggag gccgacagga actccaataa aggatacagc
      121 aacagcttcc aaaactcttt ggttagaaga taacacatct cgttgatgta ctatattaca
      181 tcccatgcca attaataagt cccttgcatc gggataaata aaactaatta cttgcgaaaa
      241 aatgttactt tcttcaacga cataagcatt caatccaaga agcctcgtag ccatgactat
      301 cttatcaggc atagaataac cggcatccgc gatattatct ttgtgtattc tcggattata
      361 attaccacta gttattaagt ataaatcccg ctcacaacga ttatccaaat caagagaact
      421 cttccttgtc atttcggaca tagacccttt gtattgtggt attttatcaa cccctaattc
      481 ctttgcagca cagagtaaac tggcagctcc acaagaattg tcatacatct gcttcctcgc
      541 caaaaaacaa ccttgttcgt ttactctatt ctcatttacc ctcacatcag tctgatgaga
      601 tatcatatat tctcttggta tagccccctg caataaaacc ccatataaca taacacctgt
      661 atgaatacat ccccgtttgc aaggcttccg ttgttccgac atcgacgagt aattgcagca
      721 cagtattgca tccagcaatg cgaactacta tgagaattag ctgaccgggt ccttattccc
      781 cgaacgaact ttaaatccaa aacctcatac tcttttccgt cgtcatcacg tccgtcacca
      841 taacaacctt tcagcatcca cccttttatt aagagttaat gtccatcagc atcagcaatt
      901 aataccgcct gatactgaca tatttttgtc gtatttacgt ccataatcgg ttattgtgat
      961 tcaggtcaca ccagcttatt tgctattgtt acaataacca ccttggttga gagctgtttt
     1021 ttcaactttg ttatcgactc ccccaactcc ttggtcagta aggctgccga ccgagcacag
     1081 tgaatagata gtatccgcag gtaccaccat gcttacttaa tactcagaag gcgaagtgct
     1141 ctccaattaa ctctgccgca ctatgcttat aagctgcttt tcaatgccgt ctatcaacat
     1201 gtccagttca ttcaatctgt tgtactggta atccaacgca gtaatgatat atgcaggtat
     1261 tctttcatcc actccagtaa cgtttcatgc aaagcattaa tttacgcgct acggaacttc
     1321 accagttgac tgagcaaacg atgtgggacc agtactgcct gcagttcttc tattgttaca
     1381 gcgatttctt taccaaacag ctgaaccacc atcaagataa agatagcctg aacattcatc
     1441 gcaccatttt gttaccaatg gcaaaagatt taacgaaccg tgcctgcatc aggcggactt
     1501 tttatccctg ctttgttagc tccctaacct tgtacagaga gcctccataa gctcccatat
     1561 caagcagaca tggttcgcag ttactgacat acaccagaaa actctgacag cagactcact
     1621 tatcctccac ttcactcata tccttattgg tgagatgaac ctgaatgaca tgttttgcga
     1681 cataaacgcc aaccagtgta tatttcacct gtagagattc tacaatctgt agcgttatgc
     1741 atctaatgtt aggctctatg atgtcagaaa tctgcgagac tctacacctt ctgcatttac
     1801 ctttatttat caccatgctc ctcagttaac ggagaacgtg ttcattgcat tttgtcactt
     1861 acatttcgcc tttggtaccg gggggatata ggttccaacg ttccgcatga gaacctgaac
     1921 cggcttcacg ccaccttgat accagatgcc gcacaggcag tcaacaagtg tcacgaacgg
     1981 tgcaatagtg atccacaccc aacgcctgaa atcagatc
//
LOCUS       DAZOZD010000429         2016 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOZD010000429 DAZOZD010000000
VERSION     DAZOZD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.435"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..678
                     /locus_tag="ACHUPB_004546"
     CDS             <1..678
                     /locus_tag="ACHUPB_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761140.1"
                     /translation="NPNHPGWISIYVRLDAPRLATLLINRHGGVLPPHLASAIQKLTG
                     TGAELVLSGSQWQSLPVLPADGTQVSFPYAGEWLAEDEIRAVLAAVRDAIRSICYQVA
                     DDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDAIVNRGAR
                     FSAVEMYLVSECVEHILSSGLACDVLRIPDEPPRRWFDRDVLREVVREARAEIRSMAD
                     ALAKIRG"
     gene            1090..1515
                     /locus_tag="ACHUPB_004547"
     CDS             1090..1515
                     /locus_tag="ACHUPB_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR0761141.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            1562..1984
                     /locus_tag="ACHUPB_004548"
     CDS             1562..1984
                     /locus_tag="ACHUPB_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR0761142.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRASRQVTVPAVMLEKVLALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
BASE COUNT          392 a          555 c          645 g          424 t
ORIGIN      
        1 aatccgaacc atccgggctg gatcagcatt tacgtgcggc tggatgcgcc ccggctggcg
       61 acgttactta tcaaccgtca cggcggcgta ctgccgccgc acctggcctc tgccattcag
      121 aaactgaccg gaaccggggc ggaacttgtt ctgtccggca gtcagtggca gtcgctgccg
      181 gtacttccgg cagacggaac gcaggtgtct ttcccgtatg ccggtgagtg gctggcagaa
      241 gacgaaatca gggctgtact tgcggcggtg cgcgatgcaa tacgaagcat ctgttaccag
      301 gtggcagacg atgcgcggcg tatccgggcg gcgctgacca ccaccggtca gacgttgctg
      361 acccgccaga cgcgccgctt tcgtctggtt gtgaaggaaa gcgatcaccc ctgctggctc
      421 gatgaagacg acgaaaatct gcccgtggtg ctcgatgcca tcgtgaaccg gggagcacgt
      481 ttttcggcag tggaaatgta cctggtcagt gagtgtgtgg agcatatcct gtccagtggg
      541 ctggcctgcg atgtgctgcg tataccggat gaaccgcccc gccgctggtt tgaccgtgat
      601 gttctgcggg aggtggtccg ggaggcccgg gccgaaatcc gcagcatggc ggatgccctc
      661 gcaaaaatcc ggggctgatg atggcgggga aaatccccgc cttttcctgc cagtccccgg
      721 acagagcaca aaggaagtcg ccgccggctc cgctttaccc ggccatgcgg tgcatggcct
      781 tgtgggtttt cagttatgtg gccgccgtgt cgtgtgcggg ctgtgccgtg cctccatctt
      841 agccgggctg gcggggatgc aagggtaaaa tgaacgcggc ggtcacccgg tccctccttc
      901 ccgtctgccg tgattttccg gtcgttcacc cgctcagatt tcgcggtctc accctgcaac
      961 tcccgtcagc cgtgtgcgtc aggctgcggc ttcccttgcg cccctgcatc cccgcctgtt
     1021 cgcccggcta agatggaggc acggcaccgc ccggtgtcgc ggaatatgtg aacaacggag
     1081 gaaaacatca tgcaatatgc gaaacctgtc actctgaacg ttgaagagtg cgaccgtctc
     1141 tcctttctgc cgtatctgtt tggcaacgat tttctgtatg ccgaggcgta tgtgtacgcg
     1201 ctggcgcaaa aaatgatgcc ggaatatcag ggcggcttct ggcacttcat ccgcctgccg
     1261 gacggtggcg gttacatgat gccggacggt gaccgtttcc acatggtgaa cggtgcaaac
     1321 tggtttgacc gtaccgtgag tgctgatgcc gcaggcatca tccttacctc ccttgtgatt
     1381 aaccgccagt tgtggctgta ccacgacagc ggggatgcag gactgaccca gctttaccgg
     1441 atgcgcgatg cgcagctgtg gcgtcacatc gaatttcacc ctgaatgcaa cgcgatttac
     1501 gcggctctgg actgattaac ggacggggcg gcaatgccgc ccctgtaaag gagacgagaa
     1561 catgtactgt actgttaaag aaattatccg cgaagtgctg gatacagacg tgccggacag
     1621 tgaatgcgtt tttgccgtgg tgctgacccg tggggatgtg cgtcacatag cccaggactg
     1681 gagtctgaca gacgatgagc tggaaaccgt catgcagcgg ctggacgatg cctttgagca
     1741 tggtgcggat gtcagcgttg ttcacgacgt tgttcgtgaa ctgatggaag aaaagcgcgc
     1801 cagccgtcag gtgacagtcc cggcggtgat gctggaaaaa gtgttggcgc tggcaggcag
     1861 tgaaatgaag cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg atgcgttcgt
     1921 cagggaagaa cgcgaagcaa tggacgttgt gttacaggcg ctggacgggg agcacatgtc
     1981 atgaatatca gcacagaaac ccgcgaaatt ctgcgc
//
LOCUS       DAZOZD010000430         2007 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOZD010000430 DAZOZD010000000
VERSION     DAZOZD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.436"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(256..1260)
                     /locus_tag="ACHUPB_004549"
                     /pseudo
     CDS             complement(256..1260)
                     /locus_tag="ACHUPB_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1245..1622
                     /locus_tag="ACHUPB_004550"
                     /pseudo
     CDS             1245..1622
                     /locus_tag="ACHUPB_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1661..>2007)
                     /locus_tag="ACHUPB_004551"
     CDS             complement(1661..>2007)
                     /locus_tag="ACHUPB_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0761143.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT          460 a          481 c          551 g          515 t
ORIGIN      
        1 tgtcagttat atttgtatga cagatgtaac aaaatgaaaa aaaagataag cacaagtgat
       61 tgattggtca gatattgatc acttggtgtt tgtattttga actttgcgat caaaatttga
      121 tctttgttgt ttccggtcaa tcctttacag gttttctgta ttatccgggg gagttaagtc
      181 tgtcgatgtc cgacagactt ttgatgatgg aaacaacatg cagcgataat gtgcatgctg
      241 ttttgcaggt ggaactcaga aggtgaactg gaactgtgcg accgcaccgt aagtgttgtc
      301 cgtgccgatc aggcctgcaa catcaaggtg aaccacatca aacggtgaga tccccacccc
      361 ggccgtgaat gcactgccgt cgttaccggc cagattctga cggtaaccgg cgcgcagctg
      421 tgcccatttc caggcattaa attctgcgcc aatggcggca aactgacggt tgctgtcgga
      481 ggtgaaaccg ctggccgggg tcagatccac atcaaatgcg gtggtgaaca tcgcgttgtg
      541 ccaggagaca ccggccgtca cctgcgggcg gatcctgaag gtttccgtga taccgttcac
      601 ttcttttgtc tcaatactac gggggatcag gttctgtgcg accagtccca gcgtccagtt
      661 gtcatccagg tccgtactgg caccgatgtc ggcgttgatg ccatttttcg tgttgtggta
      721 gcggtcgcct ttgaaggcgc tgctgtcata atttctgacc agcacgttgt agttgaacag
      781 gtcgacgcgc tggaatttgg gggggaagcc cagagaccat ttctgcccgg ccgtttccag
      841 ttccgtagcc agagcaatac ccacatcagt gatcagtgcc gcgcgggcaa aggcctgtga
      901 tgtcaggcgg cttttgtcca cctcctgccc ggtgctgttt gccacctctt ccaggtaagt
      961 gaggtcagca tcagagactt taccgtcgac actgacggtg ccgtgtgatt taaccatcag
     1021 tgcggcagag aggcgatcac cgggaagggc cgccacagca gaaactccca gctgtgcggc
     1081 ggcatgtgtg ttacggaagt cccggaggtg ttctttcagg cgtgcggcag cctcaggaac
     1141 accgtggtta ctttcgatgg cgcggtcgaa ggccttccac tcatcactga tccggtcggc
     1201 gttgtcggtg atattatccg gattggagag ctgcgcgccg acggacggaa gcaggatgct
     1261 gggctcatgc gcgacgcaaa atccacgatg tatatatcag tactaaaagc gcgacggcgg
     1321 aagaagctct gaaactaatc ggtgaactgt acgccataga gcacgatata cgcgggttac
     1381 cggtgtctga acgcctggcg gtcaggcaaa tgcagagtag accgctcctg acttccctgt
     1441 ataagctgat gcaggagaaa gaacacacgt tatcgaagaa atgtcgtctg ggagatgcgt
     1501 tccggtatat caggaagcac tgggctgcgt tgtgcaactt ctgtgatgat ggcctggctg
     1561 aggtagataa taatgcagca gaaagagcgc ttcgtgcagt ctgtctcggg aagaaaactt
     1621 tttcgaggat cactcattcc gtgtctgact gcttcagggt ttaattttgc ttctgctgac
     1681 tgaatgatga ttgggcagca agtgcaatat cccacataaa tccagttaac tcacgagcca
     1741 ccgccgtaat ggctacttgt gattttttcc cttttgctgt cagagttcgg taacgagaac
     1801 acaatcgttg ttgagctttc catgcaatat ctttggaata ttgtgttaca gagtccggtc
     1861 ggtaatatat aagccatgct ccaacttttg caggtgtacg ataagaccaa gcggcttcgt
     1921 aaagcagacg tctcagttcg ctgtttccaa cttttgttat tcctctggga cgaatactgt
     1981 ttccactgga atattcacct ggcacca
//
LOCUS       DAZOZD010000431         2006 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOZD010000431 DAZOZD010000000
VERSION     DAZOZD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.437"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..936)
                     /gene="virK"
                     /locus_tag="ACHUPB_004552"
     CDS             complement(<1..936)
                     /gene="virK"
                     /locus_tag="ACHUPB_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR0761144.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEH"
     gene            complement(941..>2006)
                     /gene="capU"
                     /locus_tag="ACHUPB_004553"
     CDS             complement(941..>2006)
                     /gene="capU"
                     /locus_tag="ACHUPB_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR0761145.1"
                     /translation="SPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIAFEASKLGI
                     DITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTWKHPFRIIR
                     QKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDFQKLYVDSR
                     NSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFCWLIVGSGS
                     PELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASAF
                     SVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQMARQAKQDI
                     EERFDINKTALKILTLAKHK"
BASE COUNT          603 a          471 c          387 g          545 t
ORIGIN      
        1 atgttctgag tttacaggat atgcatccaa agagcggata gcagtttcat ggatataatc
       61 caacaaagcg tatcgttttc gatactcaga gcgtttttta ctagctatat tgcttagcgg
      121 ctttcgaacc acctgtgttg gtaatttgta ccagtcacca caggttttcc ctgctacaga
      181 ctcccagaaa tcactgtaga cagcaacaaa ggttttgcgt ttctgatacc agtatctcaa
      241 ttgccggaaa acatgactgt gctcactgac tgccagacac tcagagacat tacaagcttt
      301 catcaacgca caaaatgctt caaaaataat ccgttttggg aaaagcccat aacaggcccg
      361 ggttgcgcac cgaataacat caggtccggt atttttgggg gctccctgta atccgccaat
      421 aaaagcggtg tgtccatttt gagtcagaat gactgaaaat gttaaccggg caatcactat
      481 tttattgaaa aacaatgaca acatcaactc tccttcccgg tcaaaaccac tgggagaaca
      541 ctggatatca aaatcctctc catctttccc gatgaattgg actaatgata ccgatttttg
      601 tggaaacaaa aattcagaat atttttctgg caaattttgc acaaagcgat aatgctcaag
      661 aatatattgc ccccgggacc aggctcgccc ccctttatgc aaataaggtc gatgaattct
      721 tgctggcaac gtgggttgta ttgccagaat atcctcaatg cactgcaagg aacgcagttc
      781 gtaataatat cgaatactgg caaccggatt tatagcacat cgcaaaatgt acatgaaccg
      841 gaattttctg tgttcccact tccccgggag tgaatctgag gaaaaaacaa tccttttaag
      901 gaaaccgata aatgataagt tacttacaga aaacatattg ttacttgtgc ttcgctaatg
      961 tgagtatttt taacgcagtt ttattaatat caaaccgctc ttctatatcc tgttttgcct
     1021 ggcgagccat ctgataaaaa cgcccagggt cattaaaaaa atcattcagg gcgcacatcc
     1081 atgcgtgctt attacctgct ggtaacaatg tcccggtctg gttgttctga ataacatcag
     1141 ggattccacc aatctgactg gccagtacag gcacagaaaa tgccgatgct tctgccagta
     1201 ccataccaaa cgattcgttt tctgaaggca gaaccaccag actggcaacc cgatatacgg
     1261 gggcggcagg aaaaacattg tcagcaataa aaacatcatc atgcatgccc atactgtcaa
     1321 tctgatactg taaatgctcc cgcagttcag gcgaaccaga ccccacaatc aaccaacaga
     1381 actgtcgtcc attcattttt aaatggaaca gtaaattcaa cataaattcg tgcccttttt
     1441 ccgggcgcaa cattcctacc tgagcaataa cggggcatcc ccttcgggac gccagccaag
     1501 aaagaacatt tggaggcaaa ctgtttcggg aatcaacgta taatttctgg aaatcaaagc
     1561 ctggaggcac aacagtaacc cgggttcgac atccttcctg ctccagatgt gtcttcatac
     1621 ttgttccggg aacaatcacc tcatcgcaaa aatgatttat tgaaaaaact tttgtttttc
     1681 gtgtcaaata tgtcttttgc ctgataattc tgaatggatg tttccaagtg aataaccgta
     1741 ctaaaccaac aatattgcta tcatgcccac tgtgacaaac gattgcattg ggctgaaaac
     1801 catgaactat tccgagtaat ctccatgcag tagggatgtg aagactgttt cgaaataacg
     1861 cgaatgtgat atcaattccc aatttactgg cttcaaaagc aattttgctg ttttccctgc
     1921 agacaagcag aactgaatgc cccattttct tcagggcctt catttgagca acagcctgta
     1981 attcctgacc acctatattc ggtgat
//
LOCUS       DAZOZD010000432         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOZD010000432 DAZOZD010000000
VERSION     DAZOZD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.438"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUPB_004554"
     CDS             <1..389
                     /locus_tag="ACHUPB_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761146.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUPB_004555"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUPB_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0761147.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUPB_004556"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUPB_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0761148.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUPB_004557"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUPB_004557"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUPB_004558"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUPB_004558"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZOZD010000433         2003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOZD010000433 DAZOZD010000000
VERSION     DAZOZD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.439"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..925
                     /locus_tag="ACHUPB_004559"
                     /pseudo
     CDS             1..925
                     /locus_tag="ACHUPB_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            936..1373
                     /locus_tag="ACHUPB_004560"
                     /pseudo
     CDS             936..1373
                     /locus_tag="ACHUPB_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1373..1750
                     /locus_tag="ACHUPB_004561"
     CDS             1373..1750
                     /locus_tag="ACHUPB_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761149.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          664 a          321 c          497 g          521 t
ORIGIN      
        1 cgataccatt gccagagttg tatcctgtat cagtcctgca aaatttcacg agtgctttat
       61 taactgaatg cgtgactgcc attcttcaga tgataaagac gtcattgcaa ttgatggaaa
      121 aacgctccgg cactcttatg acaagagtcg ccgcagggga gcgattcatg tcattagtgc
      181 gttctcaaca atgcacagtc tggtcatcgg acagatcaag acggatgaga aatctaatga
      241 gattacagct atcccagaac ttcttaacat gctagatatt aaaggaaaaa tcatcacaac
      301 tgatgcgatg ggttgccaga aagatattgc agagaagata caaaaacagg gaggtgatta
      361 tttattcgcg gtaaaaggaa accaggggcg gctaaataaa gcctttgagg aaaaatttcc
      421 gctgaaagaa ttaaataatc cagagcatga cagttacgca atgagtgaaa agagtcacgg
      481 cagagaagaa atccgtcttc atattgtttg cgatgtccct gatgaactta ttgatttcac
      541 gtttgaatgg aaaggactga agaaattatg cgtggcagtc tcctttcggt caataatagc
      601 agaacaaaag aaagagccag aaatgacggt cagatattat atcagttctg ctgatttaac
      661 cgcagaaaag ttcgccacag caatccgaaa ccactggcac gtggagaata agctgcactg
      721 gcgtctggac gtggtaatga atgaagacga ctgcaaaata agaagaggaa acgccgcaga
      781 attattttca gggatacggc acatcgctat taatatttta acgaatgata aggtattcaa
      841 ggcagggtta agacgtaaga tgcgaaaagc agccatggat agaaactatc tcgcgtcagt
      901 ccttgcgggg agcgggcttt cgtaatcttt ccctagggag tcagccgaat gttgggaagg
      961 atttgacgga tgaagagaag aactgcttgg ccggcggcag tacgggaagc ccgggaggat
     1021 ggggaccaga agatgagaat agcgcaaggg atcgtatgca gcatgaacgg tataaagatg
     1081 aattgcgaca gaaaatggga aaacccgacg ttacagattc aaatttgaaa aatattatag
     1141 acgatcttta tcggccaaat gcaaaagtag gaagtggaag cacagcagat gctgttcgtt
     1201 atgaattggc cacaggtgaa aaagttggtg gtagaggaca tgtattgaaa gcacaggatt
     1261 atacaagggc tttacaggat tggttggata agaatccaac agcatctcca ggggatcgag
     1321 cagcagcaga aaacgttctt caggatttac agaatgcttt gggaggcaag taatgaacca
     1381 atatattgct agttatatag aaaaaatggc atcattttcc actgttttgt ctgagacagt
     1441 ctcattagct aatgaatact ggcatccaga aacgccaccg gtggttatgt tgttctctca
     1501 ggttggaaaa acgattgtaa aaaattttta tgaaatgggg gatattgaaa gagaaaaaat
     1561 atttaaacat gtcgaagatg gtatggtctc gcttgatgat gaattggcca cagttgtagc
     1621 tacagggctt atagaggcaa tagtcactgc gaccgatagc aattctttac tttggcaaga
     1681 tttaagtaaa tttctgggtg acaaatcaaa gtctcatgca ttagtgtgga aaaattttgg
     1741 tcaggagtga tattattcaa gaagtattaa gctcgctatt gagatcttaa tgcttcttga
     1801 gattaaatta aagttaggac gaagtaattg caggtggaaa taaatccata gtgacgtgtg
     1861 gcaatggcta ttagatgtta tggtgtgatg ggctttggtg atttaagagc taagttagat
     1921 tgccttatga aaaagtatgg ttatatattt aatgatgtgt atcatatatt taaataaaaa
     1981 acggttctct cttgtcaaga tag
//
LOCUS       DAZOZD010000434         1981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOZD010000434 DAZOZD010000000
VERSION     DAZOZD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.440"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..713)
                     /locus_tag="ACHUPB_004562"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="ACHUPB_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="ACHUPB_004563"
     CDS             complement(766..1362)
                     /locus_tag="ACHUPB_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0761150.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..>1981)
                     /gene="yadL"
                     /locus_tag="ACHUPB_004564"
     CDS             complement(1389..>1981)
                     /gene="yadL"
                     /locus_tag="ACHUPB_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR0761151.1"
                     /translation="NLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGTCTAT
                     LVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRATCEG
                     TANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNAANAV
                     VYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
BASE COUNT          559 a          424 c          396 g          602 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagcg gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 t
//
LOCUS       DAZOZD010000435         1967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOZD010000435 DAZOZD010000000
VERSION     DAZOZD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.441"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            9..749
                     /locus_tag="ACHUPB_004565"
     CDS             9..749
                     /locus_tag="ACHUPB_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0761152.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            784..1967
                     /gene="htrE"
                     /locus_tag="ACHUPB_004566"
                     /pseudo
     CDS             784..1967
                     /gene="htrE"
                     /locus_tag="ACHUPB_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          557 a          424 c          434 g          552 t
ORIGIN      
        1 gatggtttat gttttttaac accaaacata caacagcttt atgcttcgta acctgtatgg
       61 cttttagttc atcgtctatt gcggacattg tcatttcggg tactcgcgta atatataaaa
      121 gcgatcaaaa aagtgtcaac gtacgtctgg aaaataaagg gaataacccg ttgcttgtcc
      181 agagttggtt agatactggc gataacaacg ctgagcctgg cagtattaca gtccctttta
      241 ctgccacgcc gccagtatcg cgtattgatg ccaaacgtgg gcaaacaatc aaattaatgt
      301 acacagccag cacctcactg cctaaagaca gagagagcgt gttctggttt aacgtactgg
      361 aagttccacc aaaaccagat gcagaaaagg tcgcgaatca aagtctgctg caactggcat
      421 ttcgcacacg tataaaactt ttctatcgcc cggatggatt gaagggaaat ccctctgaag
      481 ccccgttagc cctgaagtgg ttctggtcag gttcagaagg taaggcgtca ttgcgagtga
      541 ccaatccaac cccttactac gtctctttta gcagtggtga tttagaagct agcggtaaac
      601 gctatccgat tgatgtgaaa atgattgcac catttagtga tgaggtcatg aaagtcactg
      661 gccttaatgg caaagcaagt tctgcaaaag tacattttta cgccattaat gattttggtg
      721 gcgccattga aggtaatgcc agtctgtaat caggcaagga tataattccg caggaagcat
      781 aacgtgacta taaaaccaac taaaaattat cataatcatc tgacccgtat cgctacgttt
      841 tgcgcgctgc tgtattgcaa tagtgctttc tgtgctgaac ttgttgaata tgaccatacc
      901 ttcctgatgg ggcagaatgc ctcaaatatt gatctcagcc ggtatagtga aggtaaccct
      961 gctataccgg gtatgtacga cgtcagtgtt tatgtcaacg atcaacctat tattaaccaa
     1021 agcattacat ttattgaaat tgaaggaaaa aaaacgcaca agcctgtatc acacttaaga
     1081 atttattgca gtttcatata aattctcccg atataaataa cgaaaaagcc gttctgcttg
     1141 ccagggatga aacactcggc aattgcctta atttgacgga aattatccct caggcttctg
     1201 ttcgttatga cgttaacgag caacgtctgg atatagacgt tcctcaggca tgggtaatga
     1261 aaaattacca aaactatgtt gatccatcgt tatgggaaaa tggcattaat gcggccatgt
     1321 tgtcatacaa cctcaacgga tatcatagtg aaactcctgg tcgaagaaat gacagcattt
     1381 atgctgcttt taacggtggg atgaatttag gtgcatggcg actgcgtgcc tcaggcaact
     1441 acaactggat gaccgattct ggcagtaatt atgattttaa gaatcggtat atacagcgtg
     1501 atatcgcctc gctgcgttct caactcattc ttggtgagtc ttatacaacg ggcgaaacct
     1561 ttgattctgt cagtatccgc ggcattcgtt tatacagcga cagccgcatg ttgcctccga
     1621 cattagcgag ttttgcgcct atcattcatg gcgttgctaa taccaacgcc aaagtcacta
     1681 ttacgcaagg tggctataag atttatgaaa cgactgtgcc gccaggcgct ttcgtcattg
     1741 atgatctgag tccgtcaggt tacggcagcg atcttattgt tactgtcgaa gaatccgatg
     1801 gctcaaagcg gacattctcg caaccttttt catcggtcgt tcaaatgtta cgccctggcg
     1861 ttggacgttg ggatattagc ggcggccagg tcttaaaaga tgatattcag gatgagccta
     1921 atttatttca ggccagctac tactacggtc tgaataacta tctaacg
//
LOCUS       DAZOZD010000436         1960 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOZD010000436 DAZOZD010000000
VERSION     DAZOZD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.442"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="ACHUPB_004567"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="ACHUPB_004567"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0761153.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="ACHUPB_004568"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="ACHUPB_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0761154.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT          590 a          440 c          397 g          533 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc
//
LOCUS       DAZOZD010000437         1939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOZD010000437 DAZOZD010000000
VERSION     DAZOZD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.443"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUPB_004569"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUPB_004569"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR0761155.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUPB_004570"
     CDS             1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHUPB_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0761156.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          510 a          451 c          456 g          522 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgt
//
LOCUS       DAZOZD010000438         1934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOZD010000438 DAZOZD010000000
VERSION     DAZOZD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.444"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..554
                     /locus_tag="ACHUPB_004571"
                     /pseudo
     CDS             1..554
                     /locus_tag="ACHUPB_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540327.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
     gene            557..1090
                     /locus_tag="ACHUPB_004572"
     CDS             557..1090
                     /locus_tag="ACHUPB_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0761157.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1119..1646)
                     /locus_tag="ACHUPB_004573"
     CDS             complement(1119..1646)
                     /locus_tag="ACHUPB_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0761158.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(1648..>1934)
                     /locus_tag="ACHUPB_004574"
     CDS             complement(1648..>1934)
                     /locus_tag="ACHUPB_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0761159.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          537 a          420 c          429 g          548 t
ORIGIN      
        1 ggattaatta taaaaatggc ggtatttttt atcgttcagc ccgtgatggt tatggttttg
       61 aagccgactg gtccgaattt tacaccacca gaaaaccttc agcaggagat gttggtgcac
      121 tgccgttatc tggtggtcaa ctgaatggtg cactgggtat cggaacatcc agtgctcttg
      181 gcggtaattc gattgtattg ggtgataatg acacgggctt taaacaaaaa ggcgatggta
      241 atctggatgt ttatgctaat aacgtccatg ttatgcgctt tgtctccgga agcattcaaa
      301 gtaataaaac cataaatatt acgggacgtg ttaatccctc ggattacggt aactttgatt
      361 cccgctatgt gagagatgtc agacttggca cacgagttgt ttagaccatg aagaaaggcg
      421 tgatgtatga aaaatcaggc catgtaatca cggggcttgg tattatcggt gaagtcgatg
      481 gtgatgatcc ggcagtattc agaccaatac aaaagttaat taacggaaca tggtataacg
      541 tatcgcaggt ataatcatgc agcatttaaa aaacattgtc gcaggtaatc caaaaaccgt
      601 tgaacaatac cagctaacaa agaattttga tgttatctgg ttatggtccg aagatggaaa
      661 aaattggcat gaggaattaa aaaactttca ggaggataca ataaaactgg cttacacagt
      721 tgagggaata attattgcta tagacaagga tgtatcagca attaatccag aaggtttaag
      781 tgtcgttgag ttgcctgata ttacagcaaa tcgccgggct gatatttcgg gcaactggat
      841 gttcaaagat ggtgcggtga taaagcgaac ttataccgag gaagagcaaa ggcaacaagc
      901 ggaaaatgaa aagcagagcc tgctgcagct cgtcagggat aaaacccagc tgtgggactc
      961 tcagctacag ctgggcatca tttccgatga gaataaacaa aaattaaccg agtggatgct
     1021 ctatgcgcag aaggttgaat ccacagacac ctccagcctg ccagtaacgt ttcccgaaca
     1081 accagaatga gagaaggccc gctatcgggc cttaattttt attcaggctt ttgtggccat
     1141 tcaggatttg ccgtatccac acggctgacc agaacactgt agcgttccca tgcttccagt
     1201 cgtgtgcgtt cctcgtctgt tgccatattc agcctgacag ctcgttccag cggctggatg
     1261 actgattcag cttcggaaag caatgcggcc ttttgtgatt cggcctgttg ttgcagttcc
     1321 tgttctgtat attccctctt tactacttta ccatccagaa acatccattt tccgttattg
     1381 tctgcgtgac gatttgccgt aatatcagga acttcaacta tgcttagccc ttcaggatta
     1441 atagttgaaa cgtctttact gacagcaacg atgatgttgt tctcatcgta agcaattttt
     1501 atggtgtctg gctgaaaatt cttttgttcc tcataccagt ttttgccatc ttcgcaaaag
     1561 agccatacca cgcccgcttt tttagttagc tggtattgtt ccggtgtttt cggattcccg
     1621 gccttaatat ttttcaggtg catcatctta aacactcccc acgttgtacc atattccccc
     1681 aatcaatttc tgaattggcc ttctggcaac agtgtcgaca atatcatccg cattgctgtt
     1741 aattgcggct gttaaaacat aacctgacgc atcgccaaag ccgtagtcgc gccatacgtt
     1801 tttatattca atgctgccaa gacgaatatc ccgaacgaca tgattatgca gccatgtgct
     1861 taaccagttg ttttcccaca ctgaaccata aatatcgcca ctagacgcaa cttgcgcacc
     1921 acttcccagc gcaa
//
LOCUS       DAZOZD010000439         1932 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOZD010000439 DAZOZD010000000
VERSION     DAZOZD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.445"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..102)
                     /gene="prlF"
                     /locus_tag="ACHUPB_004575"
     CDS             complement(<1..102)
                     /gene="prlF"
                     /locus_tag="ACHUPB_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR0761160.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
     gene            complement(251..1822)
                     /gene="garD"
                     /locus_tag="ACHUPB_004576"
     CDS             complement(251..1822)
                     /gene="garD"
                     /locus_tag="ACHUPB_004576"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0761161.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
BASE COUNT          448 a          519 c          474 g          491 t
ORIGIN      
        1 ttttaaggcc tcacgcactg gcgcggggat agttgtttgt ccgcgtatcg tgacctttga
       61 ttcagtggtc agtacagcgt gagagcgagc attagcgggc atgattcatt gtccttttac
      121 agcctgtttt ctgctcaaat tataagcttg aactaaggta atgcaaatgc attattaatg
      181 gacgtggggg ctttaaacaa taaaaacatg gcactaaggc catgttttgc gagggacgtt
      241 ccaaaagaaa tcaggtcacc ggtgccgggt taaacaccgc cagctggtta tgcagccccc
      301 attgatccga gaaggttttc ttcttcccgc tggcgacgtc gagaataaag tggaacaact
      361 tccagcccac ctcttcaatg ctctcttcgc cggtagcgat agtgcccgcg ttaatatcca
      421 ttaaatcaaa ccagcggttc gccagctcgg tgcgggttgc cattttaatt acgggtaccg
      481 ccatcaggcc gtacggcgta ccacggccgg tcgtaaacac ttgtacggtg atacccgaag
      541 ccacctgttg cgtgccacag acaaaatcgc tggctggcgt cgcggcgtaa attaatccgc
      601 gtttagtcgg gcgttgaccg ggcgacagca cttcaacaat tgcactttta cccgatttag
      661 caatggagcc gagtgccttc tccaccacat ttgccagacc gcctttcttg ttgcccggcg
      721 aagggttggc gctgcggtcg gtttttccca tattgagata gttatcgtac cactccatct
      781 cctccagcag ccgtttgccg acctcttcgt tcactgcgcg tggtgtcagc agatggatcg
      841 cgtcacgcac ttccgttact tctgaaaaca tcaccgttgc gccgcagcgc accagtagat
      901 cagacgcata gccaaccgcc gggtttgccg ttacaccaga aaacgcatcg ctgccaccgc
      961 actgcacacc aacgaccagt tctgaagccg ggcaggtttc tcgctgccgt tgattcagtt
     1021 tttgtagatg gcgttcggcg acctgcaaaa tatcctcgac catggactga aagccgacat
     1081 gcttttcatc ctgcaaactg acaatgctgg cgctttctac tggaatagct tgcacatcat
     1141 ccgtgccgac cagcaggcgc tcaggctgca acttttcaca acccagacca atcaccatta
     1201 cttcgccgcc aaagttaggg ttcagcgaaa tattgtggat ggtacgaata ggcacaactg
     1261 ccgccggtgc gttaatcgcc acgccacagc cgtacaaatg attcagcccc accacgccat
     1321 cgacgttcgg gtatttcggt agcagatcgc gttcaatgat ttttactaca tagtccacca
     1381 cgcctgccac acagtggacg ctggtggtga tgccgagcag gtttttggtg cccacgctgc
     1441 catcggcatt gcgatagccc tcaaaggtgt atccttccag cggcggtaag ggggccggga
     1501 ctttggttgc cagtggcagc gtgtgtaacg gcggcgcttc cggtagtaca accattgatt
     1561 cgtcgatcca gcttccgcgt gggattgcgc gcacggcgta accaatcact tcgccataac
     1621 gaataatttc accattagct ggaatgtcca gcaatgcgac tttatgcccc tggggagtat
     1681 gttcaattaa ttccagccca tccggaaaac gcgttcctgc tttcaggcca ttatcattaa
     1741 caataattgc cacattatct gtgtcgtgaa cttttatata aaacgcagtt ggcgtttctt
     1801 gtctgatttc gatgttggcc attgtgcaat attctccagc caggggttat taaaatgagg
     1861 atctggtctc atttaaaaat aaatgacttc attgggtgaa ataagaatgt caggagttta
     1921 aacgcagaaa tg
//
LOCUS       DAZOZD010000440         1922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOZD010000440 DAZOZD010000000
VERSION     DAZOZD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.446"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="ACHUPB_004577"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="ACHUPB_004577"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(315..758)
                     /locus_tag="ACHUPB_004578"
     CDS             complement(315..758)
                     /locus_tag="ACHUPB_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0761162.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            915..1844
                     /gene="metA"
                     /locus_tag="ACHUPB_004579"
     CDS             915..1844
                     /gene="metA"
                     /locus_tag="ACHUPB_004579"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0761163.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          470 a          531 c          448 g          473 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc
      301 acggcagggg cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc
      361 aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc
      421 gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc
      481 tccaccagca tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc
      541 atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc
      601 cacaacggcg cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct
      661 gctgataaaa aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc
      721 ccttcctcat gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct
      781 tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat
      841 tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt
      901 gaggtaatca ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc
      961 gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac
     1021 ttaaggttct gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc
     1081 gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg
     1141 aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc
     1201 aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta
     1261 atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca
     1321 cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc
     1381 ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc
     1441 atgcgcttct gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg
     1501 actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag
     1561 aaggggatgc atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc
     1621 ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag
     1681 acccggatgt accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga
     1741 gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga
     1801 tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg
     1861 atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa
     1921 ac
//
LOCUS       DAZOZD010000441         1919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOZD010000441 DAZOZD010000000
VERSION     DAZOZD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.447"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..251
                     /gene="spa15"
                     /locus_tag="ACHUPB_004580"
                     /gene_synonym="spaK"
     CDS             <1..251
                     /gene="spa15"
                     /locus_tag="ACHUPB_004580"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR0761164.1"
                     /translation="VMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSD
                     EYLQLRVVIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            255..1547
                     /gene="spaL"
                     /locus_tag="ACHUPB_004581"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             255..1547
                     /gene="spaL"
                     /locus_tag="ACHUPB_004581"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR0761165.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKNRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            1640..>1919
                     /gene="spa13"
                     /locus_tag="ACHUPB_004582"
                     /gene_synonym="spaM"
     CDS             1640..>1919
                     /gene="spa13"
                     /locus_tag="ACHUPB_004582"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR0761166.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNF"
BASE COUNT          613 a          284 c          423 g          599 t
ORIGIN      
        1 aggttatgtt atgggctaat tttgatgcac caagtgatgt aaaacttcag tcatctgctt
       61 ataacatatt aaatttaatg ttaatgaact ttagttatag cattaatgag ctggtagagc
      121 ttcatcgttc tgatgagtac ttacagttaa gagttgttat taaagatgac tacgttcacg
      181 atgggattgt ttttgcagag attctgcatg agttttatca gaggatggaa atcttaaatg
      241 aggtcttata atcaatgagc tatacaaaat tgctcactca attatctttt cctaatagaa
      301 tctcggggcc aatcttggaa acaagtctta gcgatgtttc gattggtgag atttgtaaca
      361 ttcaggctgg aattgaaagt aatgaaattg ttgcaagagc tcaggttgta ggatttcatg
      421 atgaaaaaac aatattaagc ttgattggaa attctcgtgg actttcacgg caaacgttga
      481 ttaagcccac tgcccagttt cttcatacgc aagtcggccg tggattattg ggagcagtag
      541 tcaatccttt aggggaggtt actgataagt ttgctgttac ggataacagt gaaattcttt
      601 atcgacctgt agataatgct cctccgctat atagtgaaag ggctgcaatt gagaagcctt
      661 ttttaacagg tattaaggtt attgattctt tactcacgtg tggtgaagga cagcgaatgg
      721 ggatttttgc gtcagctggt tgtggcaaaa cttttctcat gaatatgctc attgaacata
      781 gtggtgctga tatatatgtt attgggttaa ttggtgagcg aggtcgagag gttacagaaa
      841 cggttgatta tttgaaaaac tctgagaaaa aaaacaggtg tgttttagta tatgcaactt
      901 cggattactc ttcggttgat cgttgtaatg ctgcatatat agccactgct atagccgaat
      961 tttttaggac tgaaggacat aaagtagcgc tttttattga ttcattaaca aggtatgcca
     1021 gagcattacg tgatgtggcc ttagccgctg gagaatcacc tgccagaaga ggctatccgg
     1081 tttcggtttt tgatagctta cccagacttc ttgaaaggcc aggaaagtta aaggcaggtg
     1141 gctctattac tgcattttac actgttcttt tggaggatga tgattttgct gatccattag
     1201 ctgaagaggt aagatccatt ttagatggac atatatattt gagcagaaat ctagcccaaa
     1261 aaggacaatt tcctgcaatt gattccttaa aaagtataag cagggtattt acacaggttg
     1321 ttgatgaaaa acatcgtatt atggccgctg catttcggga gctactttct gaaatagaag
     1381 agctaaggac aattattgac tttggtgaat acaaaccggg ggagaatgcc tctcaggata
     1441 aaatctacaa caaaatatct gttgttgaga gttttctgaa gcaagattat cgactcggtt
     1501 ttacttatga gcagacaatg gagcttattg gtgaaacaat tagataaggt tttaaaaatc
     1561 aaagataaat atcaacggtc tgttaaatta attgaagcac acatattaac actaaaaaaa
     1621 aaactctttg tatcgagatg tggaggcatt agataaaagg attatatact tcctgcaact
     1681 ggagaatgac ttagaacctg taggcgctca gagtgttagt cagcttttca atacaagacg
     1741 taagatcgct atagtaaaaa agcatattat ccaatatcag tctgaacgaa ttcttctaaa
     1801 gggaagaatt gaagaaattc aaaaagatat tgatgaggca aatgcttcta aaagaaaact
     1861 gcttcataaa gagagtaaaa tatgtaaacg gattggcttg attaaaagaa ataactttg
//
LOCUS       DAZOZD010000442         1901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOZD010000442 DAZOZD010000000
VERSION     DAZOZD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.448"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1650
                     /gene="tssI"
                     /locus_tag="ACHUPB_004583"
     CDS             <1..1650
                     /gene="tssI"
                     /locus_tag="ACHUPB_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0761167.1"
                     /translation="GIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVST
                     FCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKG
                     AHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELH
                     GEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFC
                     DEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDP
                     DQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQ
                     KNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSN
                     QIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYS
                     VNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLE
                     GESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
BASE COUNT          483 a          465 c          593 g          360 t
ORIGIN      
        1 ggcatcttct tttatgagga gcacgcgcaa aaaagtaccg accagagcct ggtcctgtgc
       61 gacaccgtgc gttatctgcc ggagtccttt gagatcccct ggaacccgaa cacccgtacc
      121 gaggtaagta ccttctgcat cagccagttc cgctacagcg cacaaatccg tccttcttcc
      181 gtggtgacca aagactacac ctttaaacga cccggctggg cagggcgttt tgatcaggaa
      241 ggccagtacc aggattacca gcgcacacag tatgaagtgt atgactaccc cggacgtttc
      301 aagggtgctc acgggcagaa ctttgcccgc tggcagatgg atggctggcg caacaacgca
      361 gaagtggcgc gcggaacaag ccgttctccg gagatatggc cgggacggcg aattgtgctg
      421 acggggcatc cgcaggcgaa cctgaaccgg gaatggcagg tggtggcaag tgaactgcac
      481 ggcgagcagc cgcaggcggt gccgggacgc agtggttcag gaaccacgct gaataaccac
      541 tttgcggtaa taccggcaga ccggacatgg cggccacagc cgttgctgaa accgctggtg
      601 gatggcccgc agagcgccgt cgtgacggga ccggcaggcg aggaaatctt ctgtgatgaa
      661 catggccgcg tgcgggtgaa atttaactgg gaccgctata acccgtcaaa ccaggacagt
      721 tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg gattcggtaa ccttgcgata
      781 ccgcgcgtgg gtcaggaggt cattgtggac ttcctcaacg gcgatccgga ccagccgatt
      841 attatggggc gtacctacca ccaggaaaac cgcacacccg gtagcctgcc ggggacgaag
      901 acgcagatga ccattcgttc gaaaacctat aagggcagcg ggtttaatga actgaagttt
      961 gacgatgcga cagggaaaga acaggtctac atccacgtgc agaagaacat gaacaccgag
     1021 gtgctgaata accgcaccac ggatgtgata aacaaccatg ccgaaaaaat aggtaacaac
     1081 caggcgatca ccgttaccaa taaccagatc cagaacattg gcgttaatca gatacagacg
     1141 gttggtgtca acctggtgga aacggtgggc agtaaccaga ttatcaaagt gggatcaaac
     1201 caggttgaaa aggtggggat cattcgtgcg ctgacggtgg gtgtggctta ccagacgacg
     1261 gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt cctcacaggt agggctgcat
     1321 aaatcactga tggtgggaat gggctacagc gtcaatgtag ggaataacgt caccttctcg
     1381 gtgggcaaga cgatgaagga aaacaccgga caaacagcag tttattctgc cggtgagcat
     1441 cttgaactct gctgtggtaa ggcaaggctg gtgctgacga aggacggaag catatttctc
     1501 aacggtacgc acattcatct ggaaggggag tcggatgtga acggtgatgc gccagtgatt
     1561 aactggaact gtggtgccac acaacctgta ccggatgcgc ctgtgccgaa agatttaccc
     1621 cccggaatgc cggatatgcg gcaattttga gtcaggtgca aaaaattaaa acggaactgg
     1681 tgcagtgacc agatgaacca tattaaaagc agaaggaatt gattcatggg cggcggaaaa
     1741 ccggcggcgc ggcagggcga catgacccgc aagggactgg atattgtgca gggttcagcg
     1801 ggtgtgctga taggtgcgcc gacgggagtg gcctgttcgg tgtgtccggg agggattacc
     1861 tatgctaacc cggtgaaccc ggtgctgggt gcgaaggtgc t
//
LOCUS       DAZOZD010000443         1899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOZD010000443 DAZOZD010000000
VERSION     DAZOZD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.449"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(89..643)
                     /locus_tag="ACHUPB_004584"
     CDS             complement(89..643)
                     /locus_tag="ACHUPB_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0761168.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="ACHUPB_004585"
     CDS             complement(640..930)
                     /locus_tag="ACHUPB_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0761169.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="ACHUPB_004586"
     CDS             complement(930..1529)
                     /locus_tag="ACHUPB_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0761170.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="ACHUPB_004587"
     CDS             complement(1601..1813)
                     /locus_tag="ACHUPB_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761171.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAZOZD010000444         1886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOZD010000444 DAZOZD010000000
VERSION     DAZOZD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.451"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(53..913)
                     /locus_tag="ACHUPB_004588"
     CDS             complement(53..913)
                     /locus_tag="ACHUPB_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0761172.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1096..1527)
                     /locus_tag="ACHUPB_004589"
                     /pseudo
     CDS             complement(1096..1527)
                     /locus_tag="ACHUPB_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1593..>1886
                     /locus_tag="ACHUPB_004590"
     CDS             1593..>1886
                     /locus_tag="ACHUPB_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0761173.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT          464 a          490 c          438 g          494 t
ORIGIN      
        1 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
       61 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      361 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      481 ccttcggtcc tccgatcgtt gtcagaagta agttggcagc agtgttatca ctcatggtta
      541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      661 cggcgtcaac acgggataat accgcaccac atagcagaac tttaaaagtg ctcatcattg
      721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacgaaaat
      901 gttgaatact catactcttc ctttttcaat attattgaag catttaccag ggttattgtc
      961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1021 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1081 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attttataaa ccgtggagcg
     1141 ggcaatactg agctgatgag caatttccgt tgcaccagtg cccttctgat gaagcgtcag
     1201 cacgacgttc ctgtccacgg tacgcctgcg gccaaatttg attcctttca gctttgcttc
     1261 ctgtcggccc tcattcgtgc gttctaggat cctccggcgt tcagcctgtg ccacagccga
     1321 caggatggtg accaccattt gccccatatc accgtcggta ctgatcccgt catcaatgaa
     1381 ccggactgcc acgccctgag cgtcaaattc ctttatcagt tggatcatat cggcagtgtc
     1441 gcggccaaga cggtcgagct tcttaaccag aatgacatca ccttcctcca ccttcatcct
     1501 cagcaaatcc agcccttccc ggtctgttgg gcactgttgc aaatagtcgg tggtgataaa
     1561 cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc cggcattttc
     1621 agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc agttaccgtg
     1681 agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg atttaccgct
     1741 gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg cgtaaccctt
     1801 ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc cgctgggcgt
     1861 atctgtaccg ggccgtcgac agccgg
//
LOCUS       DAZOZD010000445         1885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOZD010000445 DAZOZD010000000
VERSION     DAZOZD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.452"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUPB_004591"
     CDS             <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUPB_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR0761174.1"
                     /translation="NGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUPB_004592"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUPB_004592"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR0761175.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUPB_004593"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUPB_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR0761176.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          542 a          444 c          368 g          531 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agaga
//
LOCUS       DAZOZD010000446         1877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOZD010000446 DAZOZD010000000
VERSION     DAZOZD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.453"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHUPB_004594"
     CDS             <1..246
                     /locus_tag="ACHUPB_004594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0761177.1"
                     /translation="ANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAED
                     PKTLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            243..590
                     /gene="tnpB"
                     /locus_tag="ACHUPB_004595"
     CDS             243..590
                     /gene="tnpB"
                     /locus_tag="ACHUPB_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0761178.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            640..>1877
                     /locus_tag="ACHUPB_004596"
     CDS             640..>1877
                     /locus_tag="ACHUPB_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0761179.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFA"
BASE COUNT          422 a          533 c          560 g          362 t
ORIGIN      
        1 gccaatctgt tgttcaaatg gcgacaacaa tggcgcgagg gaaagctgct attaccttct
       61 tcagagagcc cccagctact tcctgtgact ctcgatgcag ctgccgaaca gccagaatcg
      121 ctcgcagagg acccgaaaac cctcagtatc agctgtgagg taacgttccg gcacgggacg
      181 ctccgcttca atggcaatgt cagcgaaaag ctcctgactc tgctgataca ggaactgaag
      241 cgatgatccc gttaccttcc gggatcaaaa tttggctggt tgccggtatc accgatatga
      301 gaaatggctt caacggcctg gctgcgaaag tacagacggc gctgaaagac gatcccatgt
      361 ccggccatgt tttcattttc cggggccgca gcggcagtca ggttaaactg ctgtggtcca
      421 ccagtgacgg actgtgcctc ctgaccaaac ggctggagcg tgggcgcttc gcctggccgt
      481 cagcccgtga tggcaaagtg ttccttacac aggcgcagct ggcgatgctg ctggaaggta
      541 tcgactggcg acagcctaag cggctgctga cctccctgac catgctgtaa atctctttat
      601 cctggttgtc acagaataag cccggtaaaa tacgggctta tgaacgacat ctcttctgac
      661 gacatcttcc tgctgaaaca gcgcctggcc gaacaggaag cgctgatcca cgccctgcag
      721 gaaaagctta gcaaccggga gcgcgaaata gaccatctgc aggcgcagct ggataaactc
      781 cgccggatga acttcggcag tcgttccgaa aaagtctccc gccgtatcgc acaaatggaa
      841 gccgatctga accggcttca gaaagagagc gatacgctga ctggtagggt gtatgacccg
      901 gcagtacagc gtccgttgcg tcagacccgc acccgtaagc cgttccctga atcactaccc
      961 cgtgacgaaa agcgactgtt gcctgcggcg ccgtgctgcc cgaactgcgg cggttcactg
     1021 agctatctgg gcgaggatac cgccgaacag ctggagttga tgcgtagcgc cttccgggtt
     1081 atccggacgg tacgggaaaa acatgcctgt actcagtgcg atgccatcgt gcaggcacct
     1141 gcaccttcgc ggcccatcga gcggggtatc gccggaccgg ggctgctggc ccgcgtgctg
     1201 acctcgaagt atgcagagca caccccgctg tatcgccagt cagaaatata cggccggcaa
     1261 ggtgtggagc tgagccgttc actgctgtcg ggctgggtgg atgcatgctg ccggctgctg
     1321 tctccgatgg aagaggcgct tcatggctat gtgctgactg acggtaagct ccatgttgat
     1381 gacacgcctg tcccggtgct gttgccaggc aataagaaaa cgaagaccgg gcggttgtgg
     1441 gcgtatgttc gtgacgaccg taacgccggg tcaacgctgg cgccggcggt gtggttcgct
     1501 tacagcccgg acagaaaagg catccatccg cagacccatc ttgcggggtt cagtggtgta
     1561 ctgcaggcgg atgcatacgc cgggttcaac gagctgtacc gggatggccg gataacggaa
     1621 gccgcctgtt gggctcacgc ccgccgtaaa atccacgatg tgcacgttcg caccccgtca
     1681 gccctgacgg aggaagcgct gaaacggatc ggcgaactgt acgccatcga ggcagagata
     1741 aggggaatga cggcggaaca gcgccttgcc gaacgtcagt tgaaaacgaa accgctgctg
     1801 aaatccctgg aaagctggct gcgtgaaaag atgaaaaccc tgtcgcgaca ctcagaactg
     1861 gcgaaagcgt tcgcata
//
LOCUS       DAZOZD010000447         1870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOZD010000447 DAZOZD010000000
VERSION     DAZOZD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.454"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>1870
                     /locus_tag="ACHUPB_004597"
     CDS             <1..>1870
                     /locus_tag="ACHUPB_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFR0761180.1"
                     /translation="DPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPPAW
                     SSFGNKTIWGGNEWVDDSPTRSDIEKRDKEIAAYKNTLSAQQKENENKRTEAGKRLSA
                     AIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHTES
                     RMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRLSE
                     LEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQQL
                     TQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNYLL
                     THSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAESAV
                     NSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATKDA
                     INFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINKKINA
                     KDRAAIAAALESVKLSDISSNLNRFSRGLGYAGKFTSLADWITEFGKAVRTENWRPLF
                     VKTEAIIAGNAATALVALVFSILTGSALGIIGYGLLMAVTGALIDESLVEKANKFWG"
BASE COUNT          582 a          404 c          486 g          398 t
ORIGIN      
        1 gaccctgtac gtattacaaa tcccggtgca gaatcgctgg ggtatgattc agatggccat
       61 gaaattatgg ccgttgatat ttatgtaaac cctccacgtg tcgatgtctt tcatggtacc
      121 ccgcctgcat ggagttcctt cgggaacaaa accatctggg gcggaaacga gtgggtcgat
      181 gattccccaa cccgaagtga tatcgaaaaa agggacaagg aaatcgcagc gtacaaaaac
      241 acgctcagcg cgcagcagaa agaaaatgag aataagcgca ctgaagccgg aaaacgcctc
      301 tctgcggcga ttgctgcaag ggaaaaagat gaaaacacac tgaaaacact ccgtgccgga
      361 aacgcagatg ccgctgatat tacacgacag gagttcagac tcctgcaggc agagctgaga
      421 gaatacggat tccgtactga aatcgccgga tatgacgccc tccggctgca tacagagagc
      481 cggatgctgt ttgctgatgc tgattctctt cgtatatctc cccgggaggc caggtcgtta
      541 atcgaacagg ctgaaaaacg gcagaaggat gcgcagaacg cagacaagaa ggccgctgat
      601 atgcttgctg aatacgagcg cagaaaaggt attctggaca cgcggttgtc agagctggaa
      661 aaaaatggcg gggcagccct tgccgttctt gatgcacaac aggcccgtct gctcgggcag
      721 cagacacgga atgacagggc catttcagag gcccggaata aactcagttc agtgacggaa
      781 tcgcttaaca cggcccgtaa tgcattaacc agagctgaac aacagctgac gcaacagaaa
      841 aacacgcctg acggcaaaac gatagtttcc cctgaaaaat tcccggggcg ttcatcaaca
      901 aatcattcta ttgttgtgag cggtgatccg agatttgccg gtacgataaa aatcacaacc
      961 agcgcagtca tcgataaccg tgcaaacctg aattatcttc tgacccattc cggtctggat
     1021 tataaacgca atattctgaa tgaccggaat ccggtggtga cagaggatgt ggaaggtgac
     1081 aagaaaattt ataatgctga agttgctgaa tgggataagt tacggcaacg attgcttgat
     1141 gccagaaata aaatcacctc tgctgaatct gcggtaaatt cggcgagaaa taacctcagt
     1201 gccagaacaa atgagcaaaa gcatgcaaat gacgctctta atgccctgtt gaaggaaaaa
     1261 gagaatatcc gtaaccagct tgccggcatc aatcagaaga tagcggaaga gaaaagaaaa
     1321 caggatgaac tgaaggcaac gaaagacgca attaatttca caacagagtt cctgaaatca
     1381 gtttcagaaa aatatggtgc aaaagctgag cagttagcca gagagatggc cgggcaggct
     1441 aaagggaaga aaatacgtaa tgttgaagag gcattaaaaa cgtatgaaaa gtaccgggct
     1501 gacattaaca aaaaaattaa tgcaaaagat cgtgcagcga ttgccgcagc ccttgagtct
     1561 gtgaagctgt ctgatatatc gtctaatctg aacagattca gtcggggact gggatatgca
     1621 ggaaaattta caagtcttgc tgactggatc actgagtttg gtaaggctgt ccggacagag
     1681 aactggcgtc ctctttttgt taaaacagaa gccattatag caggcaatgc cgcaacggct
     1741 cttgttgcac tggtcttcag tattcttacc ggaagcgctt taggcattat cgggtatggt
     1801 ttactgatgg ctgtcaccgg tgcgctgatt gatgaatcgc ttgtggaaaa agcgaataag
     1861 ttctggggta
//
LOCUS       DAZOZD010000448         1857 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOZD010000448 DAZOZD010000000
VERSION     DAZOZD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.455"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..186
                     /locus_tag="ACHUPB_004598"
     CDS             <1..186
                     /locus_tag="ACHUPB_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR0761181.1"
                     /translation="LVLELAREEFDYLSAEEIAEEIRRDSERGYCCHGFERNCCPLGC
                     GDLGDDCCDAADLMEEP"
     gene            183..1061
                     /locus_tag="ACHUPB_004599"
     CDS             183..1061
                     /locus_tag="ACHUPB_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0761182.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDTVSPEFMRALAERLPQHEDKLMTIAERLEQKGALEKALAIARQLQKMGMTPEQI
                     KQATGLSEAELKKIIH"
     gene            1120..1326
                     /locus_tag="ACHUPB_004600"
     CDS             1120..1326
                     /locus_tag="ACHUPB_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761183.1"
                     /translation="MTIAERLIQKGFDEGFKESFKEGFKEGALEVAREIACRLRDMGW
                     PPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(1357..1608)
                     /locus_tag="ACHUPB_004601"
     CDS             complement(1357..1608)
                     /locus_tag="ACHUPB_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0761184.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(1642..1838)
                     /locus_tag="ACHUPB_004602"
                     /pseudo
     CDS             complement(1642..1838)
                     /locus_tag="ACHUPB_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          438 a          464 c          504 g          451 t
ORIGIN      
        1 ctggtcctgg aactggcccg tgaggagttt gattaccttt ccgcagaaga gattgctgaa
       61 gaaatacgcc gtgactcaga acgcggctat tgttgtcatg gctttgaacg caattgctgt
      121 cctctcggtt gtggggattt gggcgatgat tgctgcgatg ctgctgattt gatggaggaa
      181 ccgtgagtaa gaagaaaaac accacaacgc ccacgccgca tgatgccgcg ttccggtcgt
      241 tcctggcgaa tcccgacgtc gccagagatt ttctggaact gcatcttcct gcggagtacc
      301 ggcagttgtg cgacctgtcc acgctgaagc tggaacccgc cacctttgta gagccggacc
      361 tgcatcagta cgccagcgat atcctctgga gcgtgaaaac caccgggggt gaagacggct
      421 atgtttatac gctcatcgag caccagagca cagaaaatct gtacatgcct tttcgcatgt
      481 tacgctacag tgtggcggcg atgcagagac atctggagca gcacaaaacg ttgccactgg
      541 tgattccggt gctgttctat cacggtgagc gcagcccgta cccgtacagc atgaactggc
      601 tggactgttt tgagaatccg gcacttgcgg ctaaaatata cacaaagccg tttccgctgg
      661 ttgatatcac tgtcgttgat gacaatgaaa tcatgaacca tcgccggatg gccgcactga
      721 cgctgctgat gaagcatatc cgccatcgtg acatgatgga gctgctggac aaactcccgc
      781 aggtcatggt ggagatttca gacgagcagg tgcgtgttct gattcattac atcgttaacg
      841 caggggacac tgtatcaccg gaatttatgc gggcgctggc cgagcgcctg ccgcagcatg
      901 aggataaact gatgacaatc gcagaacgtc ttgagcagaa aggtgcgctt gaaaaggccc
      961 tggccattgc gcgtcagctt cagaaaatgg ggatgacacc tgagcagatt aagcaggcta
     1021 ccggactgtc cgaagctgaa ctgaaaaaaa tcattcactg atgagagcat cacagcccgg
     1081 cagaatgccg ggcttttttg ccagcagggg cagagcagta tgactattgc agaacgtctt
     1141 atacagaaag gtttcgacga aggttttaaa gaaagtttta aagaaggctt taaagaaggg
     1201 gcgcttgaag tggcgcggga aatagcctgc cggcttcggg atatgggctg gccgccggaa
     1261 cggattcagg aggcaaccgg actttccggt gaagaactga aaaaactgtt tcctgatgag
     1321 cagtagcctg gcattcagcc aggctgtgaa ttacgctcac acatcctctg ccgtttcccc
     1381 cagttcattt tccagtcgcc tgatgatatc tgccttgcgc atcccctgca tgtgaggcag
     1441 caggtcacgg actgaaagtg cctgggtggt cctccagacg gtgacatcca cctgctccgc
     1501 attttcccag gcgtgccatg ttctctgtgt cacgccaaat tctctggccg ctttttcgcg
     1561 tgaccagagc atgctttttc gccagattcg cagttcccag ccggtcataa agtgcctttt
     1621 aaaaagtgaa aaaacttcac tttattttat catcataatc accaggtcag cgcgataaat
     1681 aagcgtgaaa aagtgaagaa atttcacttt ttttagttgt agatgtcgtg ctgttttctc
     1741 tgttttcacc tgtgcgggag tgggtgtcag tgaccaccac atgccattgt tgtgccgtcg
     1801 tttttcttca gtcgcgccgg atgcagcgct ccctgcacac cttacggtgt gtaaggc
//
LOCUS       DAZOZD010000449         1852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOZD010000449 DAZOZD010000000
VERSION     DAZOZD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.456"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUPB_004603"
     CDS             <1..184
                     /locus_tag="ACHUPB_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761185.1"
                     /translation="HPARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQPKKVKFLH
                     FFALVYRADLAIVMIK"
     gene            218..469
                     /locus_tag="ACHUPB_004604"
     CDS             218..469
                     /locus_tag="ACHUPB_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFR0761186.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(500..706)
                     /locus_tag="ACHUPB_004605"
     CDS             complement(500..706)
                     /locus_tag="ACHUPB_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761187.1"
                     /translation="MTIAERLIQKGFDEGFDEGFKEGFKKGALEVAREAACRLRDMGW
                     TPERIQEAAGLSGEELKKLFPDEQ"
     gene            complement(768..1670)
                     /locus_tag="ACHUPB_004606"
     CDS             complement(768..1670)
                     /locus_tag="ACHUPB_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0761188.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKITGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGALEKALAIACQLQK
                     MGMTPEQIKQATGLSDDELKKIIH"
     gene            complement(1667..>1852)
                     /locus_tag="ACHUPB_004607"
     CDS             complement(1667..>1852)
                     /locus_tag="ACHUPB_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR0761189.1"
                     /translation="LVLELAREEFDYLSAEEIAEEIRRDSERGYCCHGFERNCCPLGC
                     GDLGDDCCDAADLMEEP"
BASE COUNT          445 a          506 c          459 g          442 t
ORIGIN      
        1 gcatccggcg cgactgaaga aaaacgacgg cacaacaatg gcatgtggtg gtcactggca
       61 caccactccc gcacaggtga aaacagagaa aaccgcacga catttacaac caaaaaaagt
      121 gaaatttctt cacttttttg cacttgttta tcgcgctgat ttggcaattg tgatgataaa
      181 ataaagtgaa gttttttcac tttttcaaag gtgttttatg actggctggg aactgcgaat
      241 ctggcgaaaa agcatgctct ggtcacgcga aaaagcggcc agagaatttg gcgtgacgca
      301 gagaacatgg cacgcctggg aaaatgcgga acaggtggac gtcaccgtct ggaggaccac
      361 ccaggcactt tcagtccgtg acctgctgcc tcacatgcag gggatgcgca aggcagatat
      421 catcaggcgt ctggaaaatg aactggggga aacggcagag gatgtgtgag cgtgattcac
      481 agcctggcag aatgccaggc tactgctcat caggaaacag ttttttcagt tcttcaccgg
      541 aaagtccggc tgcctcctga atccgttccg gcgtccagcc catatcccgc agccggcagg
      601 ctgcttcccg cgccacttca agcgcacctt tcttaaaacc ttctttaaaa ccttcgtcga
      661 aaccttcgtc gaaacctttc tgtataagac gttctgcaat cgtcatactg ttttctctgc
      721 tcctgctggc aaaaggcccg gtattttgcc gggctgtgag gttctcatca gtgaatgatt
      781 ttcttcagtt cgtcatcgga aagtccggta gcctgcttaa tctgctccgg cgtcatcccc
      841 attttctgaa gctggcacgc aatagccagg gctttttcaa gcgcaccttc catcctgccc
      901 tcctggcgac ctttttgctc aagacgttct gcgatagtca tcagtttatc ctcatgctgc
      961 ggcagacgct cagccagcgc ccgcataaat tccggtgata cagagtcccc tgcgttaacg
     1021 atgtaatgaa tcagaacacg cacctgctcg tctgaaattt ccaccatgac ctgcgggagt
     1081 ttgtccagca gctccatcat gtcacgatgg cggatatgct tcatcagcag cgtcagtgcg
     1141 gccatccggc gatggttcat gatttcattg tcatcaacga cagtgatatc aaccagcgga
     1201 aacggctttg tgtatatttt agccgcaagc gccggattct caaaacagtc cagccagttc
     1261 atgctgtacg gatacgggct gcgctcacca tgatagaaca gcaccggaat caccagtggc
     1321 aacgttttgt gctgctccag atgtctctgc atcgccgcca cactgtaacg taacatgcgg
     1381 aaaggcatgt acagattttc ggtgctctgg tgctcgatga gcgtataaac atagccgtct
     1441 ttacccccgg tgattttcac gctccagagg atatcgctgg cgtactgatg caggtccggc
     1501 tctacaaagg tggcgggttc cagcttcagc gtggacaggt cgcacaactg ccggtactcc
     1561 gcaggaagat gcagttccag aaaatctctg gcgacgtcgg gattcgccag gaacgaccgg
     1621 aacgcggcat catgcggcgt gggcgtcgtg gtgtttttct tcttactcac ggttcctcca
     1681 tcaaatcagc agcatcgcag caatcatcgc ccaaatcccc acaaccgaga ggacagcaat
     1741 tgcgttcaaa gccatgacaa caatagccgc gttctgagtc acggcgtatt tcttcagcaa
     1801 tctcttctgc ggaaaggtaa tcaaactcct cacgggccag ttccaggacc ag
//
LOCUS       DAZOZD010000450         1829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOZD010000450 DAZOZD010000000
VERSION     DAZOZD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.458"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1767
                     /locus_tag="ACHUPB_004608"
     CDS             <1..1767
                     /locus_tag="ACHUPB_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0761190.1"
                     /translation="ESVKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLAERGIIP
                     ADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIGDLLGYS
                     PRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQSGWGGK
                     PEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCVALDAAL
                     LEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEEERLNEL
                     MARYDALENQCEESDLLEAEMKLMRCIAKVRAWTPEMRAGSGVVVSWRYGNVCVQRGV
                     QLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALL
                     AWTLCLNVFGSGAYSKPAQVSLECKHYSLTSDAPSGKEGAAFLALMAEKVRLAALLPE
                     GWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAIGFHMRD
                     WWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPG
                     WMCSPRPQTDATERADNLADAA"
BASE COUNT          406 a          482 c          599 g          342 t
ORIGIN      
        1 gagtccgtta agggcgttgg cctgttgcag aatctggtcg tgcataccct gcctggtgac
       61 cgttacggtg tcgccgcagg tggtcgccga ctggcagcac tcaacatgct ggcagagcgt
      121 ggcatcattc cggctgactg gcctgtacgc gtgaaggtta ttccgcagga gctggcgacg
      181 gctgcatcaa tgactgaaaa cggtcatcgt cgggatatgc accctgccga acagattgcc
      241 ggatttcgtg caatggcgca ggaaggcaaa acgcctgcac aaatcggtga tttgctgggc
      301 tattcgcccc gccacgttca gcgaatgctg aaactggcag accttgcgcc tgtcatcctc
      361 gatgcgctgg cagaagaccg catcaccaca gagcactgtc aggcgctggc gctggagaac
      421 gacaccgcgc gtcaggtgca ggtgtttgaa gccgcctgcc agtcgggatg gggcggtaaa
      481 ccggaagtgc agaccattcg tcgtctggtg accgaaagtg aagtggcggt ggcggggaac
      541 agtaaattcc gcttcgtggg ggctgatgcc ttctcgccag acgaactgcg caccgatttg
      601 ttcagcgacg acgagggtgg ctatgtggac tgtgttgcgc tcgatgctgc cctgctggaa
      661 aaactccagg ctgtcgctga acaccttcgg gaagccgaag gctgggaatg gtgcgccgga
      721 cgtatggagc ctgttggttc ctgccgtgag gatgccggaa cataccgcag tctgccggag
      781 ccggaagcgg tgctgacgga ggcagaagaa gaacgcctga acgaactgat ggcacgttac
      841 gacgcgctgg aaaaccagtg tgaggaatcc gacctgctgg aagcagaaat gaagctgatg
      901 cgctgtatag cgaaggtcag ggcgtggacg ccggaaatgc gtgccggaag cggtgtggtg
      961 gtgtcctggc gttacggtaa tgtgtgtgtc cagcgtggtg tgcagttgcg cagtgaggat
     1021 gacgtggctg acgacgctga ccgcacggaa caggtgcagg aaaaagcgtc agtggaggaa
     1081 atcagcctgc cgttgctgac gaaaatgtcc tcagagcgca cgctggcagt ccaggcagca
     1141 ctgatgcagc aaccggacaa atctctggca ctgctggcat ggacgctctg cctgaatgtg
     1201 tttggcagcg gagcgtacag taaaccagca caagtcagcc tggaatgtaa acattattcg
     1261 ctgaccagcg atgcgccgtc agggaaggaa ggtgccgcat tcctggcgct gatggcagaa
     1321 aaagtccgtc ttgcagccct gctgccggag ggatggtcac gggacatgac gacgttcctg
     1381 tcactcagtc aggaggtgct gttatccctg ctcagcttct gtaccgcgtg cagtcttaac
     1441 ggtgtccaga cccgcgagtg tggtcacacg tcacgcagtc cgcttgactc gctggaaagc
     1501 gccatcggct ttcacatgcg tgactggtgg cagccgacaa aagcaaactt cttcggacac
     1561 ctgaaaaagc cgcagattat cgcagccctg aatgaggcag ggctgtccgg tgccgcacgg
     1621 gacgcggaga agatgaagaa aggcgatgcg gctgaacatg cagagttcca tatgaaagac
     1681 aaccgctggg tgcctggctg gatgtgttca ccacgtccac agacggatgc cactgaacgc
     1741 gccgataacc tggctgatgc cgcctgatga acaaccacac cgccccgccg gagacggggc
     1801 ggcagcaagg gagataccgt gatgaaaac
//
LOCUS       DAZOZD010000451         1829 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOZD010000451 DAZOZD010000000
VERSION     DAZOZD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.459"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1767
                     /locus_tag="ACHUPB_004609"
     CDS             <1..1767
                     /locus_tag="ACHUPB_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR0761191.1"
                     /translation="DSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLAERNILP
                     ADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIGDLLGYS
                     PRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQSGWGGK
                     PDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRVALDAAL
                     LEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEEERLNEL
                     MTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNVCVQRGV
                     QLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLALL
                     AWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARLAALLPE
                     GWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAIGFHMRD
                     WWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKDNRWVPG
                     WMCSPRPQTDATERADNLADAA"
BASE COUNT          412 a          491 c          589 g          337 t
ORIGIN      
        1 gattccatta agggagtcgg cctgctgcag aatctggttg ttcatgccct gccaggtgac
       61 cgttacggtg tcgccgcagg cggtcgccga ctggcagcac tcaacatgct ggcagagcgc
      121 aacatccttc cggctgactg gcctgtacgc gtgaaggtca ttccgcagga gctggcgact
      181 gccgcatcaa tgactgaaaa cggtcatcgt cgggatatgc accctgccga acagattgcc
      241 ggattccgtg cgatggcgca ggaaggcaaa acgcctgcac aaatcggtga tttgctgggc
      301 tattcgcccc gccacgttca gcgaatgctg aaactggcag accttgcgcc tgtcatcctc
      361 gatgcgctgg cagaagaccg catcaccacc gagcactgtc aggcgctggc gctggagaac
      421 gacaccgcgc gtcaggtaca ggtgtttgaa gccgcctgtc agtcgggatg gggcggaaaa
      481 ccggatgtgc gggttatccg caatctgatt acggaaagtg aagtggcggt ggcggggaac
      541 agtaaattcc gcttcgtggg ggctgatgct ttctcgccag acgaactgcg cactgatttg
      601 ttcagcgatg acggggacgg ttatgtcgac cgcgtggcgc tcgatgctgc cctgctggaa
      661 aaactccagg ctgtcgctga acaccttcgg gaagccgaag gctgggaatg gtgcgccgga
      721 cgcatggagc ctgtcggtga gtgccgtgag gatgccggaa cataccgctg tctgccggag
      781 ccggaagcgg tgctgacgga ggcggaagaa gaacgcctga acgaactgat gacgcgttac
      841 gacgcgctgg aaaaccagtg tgaggaatcc gacctgctgg aagcagaaat gaagctgatg
      901 cgctgcatgg cgaaggtcag agcgtggacg ccggagatgc gtaccggaag cggtgtggtg
      961 gtgtcctggc gttacggtaa tgtgtgtgtc cagcgtggtg tgcagttgcg cagcgaggat
     1021 gacgcggctg acgacgctga ccgcacggaa caggtgcagg agaaagcatc agtggaggaa
     1081 atcagtctgc cactgctgac gaaaatgtct tccgaacgca cgctggcagt ccaggcggca
     1141 ctcatgcagc aaccggacaa atccctggca ctgctggcat ggacgctctg cctgaatgtg
     1201 tttgacagcg gagcgtacag taaaccagca caaatcagcc tggaatgtaa acattattcg
     1261 ctgaccagcg atgcgccgtc agggaaggaa ggtgccgcat tcctggcgct gatggcagaa
     1321 aaagcccgtc ttgctgccct gttaccggag ggatggtcac gggacatgac gacgttcctg
     1381 tcactcagtc aggaggtgct gttatccctg ctcagtttct gcaccgcatg cagccttaac
     1441 ggtgtccaga cccgcgagtg tggtcgcacg tcacgcagtc cgcttgactc actggagagc
     1501 gccatcggtt ttcacatgcg cgactggtgg cagccgacga aagcaaactt cttcggacac
     1561 ctgaaaaagc cgcagattat cgcagccctg aatgatgcag gactgtccgg tgccgcacgg
     1621 gacgcagaga agatgaagaa aggcgatgcc gctgaacatg cagagttcca tatgaaagac
     1681 aaccgctggg tgcctggctg gatgtgttca ccacgtccac agacggatgc cactgaacgc
     1741 gccgataacc tggctgatgc cgcctgatga acaaccacac cgccccgccg gagacggggc
     1801 ggcagaggga gaaatacgat gatgaatac
//
LOCUS       DAZOZD010000452         1818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOZD010000452 DAZOZD010000000
VERSION     DAZOZD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.460"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUPB_004610"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHUPB_004610"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0761192.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1006..1416
                     /locus_tag="ACHUPB_004611"
     CDS             1006..1416
                     /locus_tag="ACHUPB_004611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761193.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(1450..>1818)
                     /locus_tag="ACHUPB_004612"
     CDS             complement(1450..>1818)
                     /locus_tag="ACHUPB_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0761194.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAZOZD010000453         1815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOZD010000453 DAZOZD010000000
VERSION     DAZOZD010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.461"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..259)
                     /locus_tag="ACHUPB_004613"
     CDS             complement(<1..259)
                     /locus_tag="ACHUPB_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFR0761195.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            474..1664
                     /locus_tag="ACHUPB_004614"
                     /pseudo
     CDS             474..1664
                     /locus_tag="ACHUPB_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          487 a          434 c          430 g          464 t
ORIGIN      
        1 aaattttatc cgcgtggtgc atcagcacaa atttatccca caactgttct tctgtctcga
       61 catgcgccgg atctttcaca atagtattgg ggatcgggca caccttctgg caggttggtg
      121 tctcgtagtg ccctacgcat tcggtacact tatcgctgtt aatctcgtag atatgatctc
      181 ccattgaaat cgcctcattc gggcattcgg gttcacacat atcacaatta atacagcgtt
      241 tagtaattag taaagacatt tcaatggatt accgttaaat cattttaaaa tcagtaagtt
      301 gtatcgagtt tgtatgcttt actgtcatta acttactgta tgttgatcca gtgtatttaa
      361 ccttgataaa ctcagtccag caacacaaaa ccgcaacaca ttgcattttg tcccgtagaa
      421 aagacttgta tgtgtgagct tgttttctgc gcctacgcag ataaggattg agaatgccgc
      481 gcactgtaac acataatccg gatagcccca ataatgacga tgttttagcc gcatctgaaa
      541 aatgggacgc ctgtaaaccc ccctatacca gcgcacacat gaaaatctgt gttgctgccg
      601 ccaaaatcat cctcgctgct tccggcgtgg ctcgccgttc caaatacgaa aaagagaact
      661 atctccgtat cgatttcagc aaagccggta aggttacatt ttacgccgag tttccaaaaa
      721 agatgggcct caaaggtaaa aagctcggcg agtggccgga gctcgctatc cagctggcgc
      781 gcgaaaaagc actaggtatg gctgacggtg gactgcgggc agagtccgta catgcagcgc
      841 tggaaatgta ccgggatgac ctcaaagcca aagtagcccg gcagaagctg agcccggaca
      901 gtttcacaac ctacggggtg cgtatcgacc ggattaaagc aacgttcggc gagcgtgagg
      961 tgttcagcga cgtaacatac aatcggctgg tggaagtgct ggacgagtgg atcgccactc
     1021 gctcgaacaa taacgccctg gagttgtttg ccgagctccg tcggttctgg aagttctgcg
     1081 cacctactct ttgcaacggc cgcaatgttg ccgccagtct gccagatgat tatgtttcct
     1141 cccgcgtaca gaaacctacc cccacacggc tttttaccga tattgaatca atcgcccgac
     1201 tctggctcaa tgttgctgcc tgcacctctg tacaccagaa gaatgctgtt cgcttcatga
     1261 tcatcactgg tgttcgtccg attaatgtcc ataacctgcg ctgggactac gtttacgagg
     1321 aggctggtga aattgtttat ccggaagggg ttatcggcat gcgaggggct atgaaaacac
     1381 aaaaggcttt ccgcctgccg ataacgcctg agatccggcg gattatcgac gagcagaaag
     1441 cctggcgtga ttcagttcct gagtgcaaca gggattatgt atttttgcag ccacgtgatc
     1501 caatgcagcc attttcaaaa cgatcactgg ataagctggt gaaaacatac agcccggacg
     1561 gggctgtaaa aggaataaaa catgatggga ctgttaaagg gaaagacggt gcatttaata
     1621 cgatgtgccg taaattcctt aagagcaatg ttattgcctt gatcgatagg aattaaaacc
     1681 ccaaaaagat taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctcata
     1741 tgaacggaac cgacgattaa actggatggc tctgattgat tcagggtatg aatggcggtt
     1801 ttttgctccg tttcc
//
LOCUS       DAZOZD010000454         1812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOZD010000454 DAZOZD010000000
VERSION     DAZOZD010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.462"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(56..1705)
                     /locus_tag="ACHUPB_004615"
     CDS             complement(56..1705)
                     /locus_tag="ACHUPB_004615"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0761196.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          417 a          491 c          475 g          429 t
ORIGIN      
        1 cctgaagagg ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg
       61 gttcgccgta caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct
      121 gcaacggata ctcgccaccg ccgccttcgc ggggaacacc atcggcaata tggtatttct
      181 cgatcatttt gtgctgttcc agatagaact gattcaccgt tttcagccag ctgcgcgcga
      241 tttcatcacc cagaaggtca tcgccataca ttttaaatcc ctgaattgcc atccattgta
      301 acggtgccca gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccagaa
      361 tcccgccagg tgtcagtaac cggctgcgta cggcgtttgc cagacgatcg gcctgttcat
      421 ggttcgccat gccgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgtt
      481 cgcgtcgcca gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta
      541 ccgcatcgcg acgggcactg gccttctggc ggaacagcgc ttctgtctct ttctcgcctt
      601 tcagcgccga gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga
      661 tggggatgaa ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg
      721 aagagtaatc ccaaccggag gccgcccccg cgcgtaaatc gcggtacacc tcgttgggcg
      781 ggcgaccaga atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt
      841 cgcgatcgtc ccagtaacgg ttgagcagcg atccgtccgg catccgcaca acatggcgat
      901 aggcctgatt agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttaa
      961 ggtggtcgag atagcggcgc gcaccgcgta caccatcttc ttcaaacaac tccaccatca
     1021 gcgcaaaaac cggtggttgc gatcggctca aataataggt gcggttgccg ttggggatgt
     1081 gaccatagtt ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc
     1141 gaccactttc cgccagcccc agcatggtga aataggagtc ccaatagtag gtttcgctaa
     1201 aacggccgcc tgggacaata tatgactgcg gcagcgccag tagagaagac cacggaatgt
     1261 gatcctgtgg ttcgcgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat
     1321 tttgcgggtc cgatacatac tcgctggagt agacctccgg cagccagaag tgattttcaa
     1381 caaacttgcg caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat
     1441 ccagcgggtc cattttaggt gcgcagtcgg gaaaggtttt actgtccggg aaaattttcg
     1501 ccgactgaac atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg
     1561 cgggcagccc ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta
     1621 attcatacgg gtccagctca tagcagagat cgacttcgat catcagttcg tctggattag
     1681 ggttttgaat tttctgattg agcataagga tccaacctcc gaaattcgtc gctaattatc
     1741 gggcttttct ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga
     1801 tcacgtttat ta
//
LOCUS       DAZOZD010000455         1811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOZD010000455 DAZOZD010000000
VERSION     DAZOZD010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.463"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..256
                     /locus_tag="ACHUPB_004616"
     CDS             <1..256
                     /locus_tag="ACHUPB_004616"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0761197.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            315..1772
                     /gene="dtpC"
                     /locus_tag="ACHUPB_004617"
     CDS             315..1772
                     /gene="dtpC"
                     /locus_tag="ACHUPB_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR0761198.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT          390 a          432 c          475 g          514 t
ORIGIN      
        1 gaccgaagaa gtttacctcg acgaaatggt aggtcgtatt aacgccaata tgatccttcc
       61 gtatccgccg ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc
      121 ggttctggag ttcctgcaga tgctgtgtga aatcggcact cactatccgg gatttgaaac
      181 cgatattcac ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga
      241 agaaagcaaa aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct
      301 cataaggaga acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga
      361 tccaaatctg ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca
      421 cccatcagct tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc
      481 tggtttacgt tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca
      541 ctgcagtgat tgccggcgcg ctgttaatga cccttggcca tgtggtgctg ggcattgata
      601 caaattcaac ctttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat
      661 tcaaatcaaa catcagctgt ttgcttggcg agctctacga cgagaacgat catcgacgtg
      721 atggcggttt ttcgctgctg tatgctgcgg gcaatatcgg ttctatcgcc gccccaatcg
      781 cctgcggcct ggctgctcag tggtatggat ggcatgttgg ctttgccctt gcgggtggcg
      841 gcatgtttat cggtttgttg attttcttaa gcggtcatcg tcatttccag tccacgcgta
      901 gtatggataa aaaagcgctc actagcgtca aatttgcctt accagtatgg agctggttag
      961 tggtgatgct ctgtttagcc ccagtatttt ttaccctgct gctggagaac gactggtccg
     1021 gatatttgct ggcgatcgtt tgcctcattg ccgcacaaat cattgcccgc atgatgatca
     1081 aattccccga acaccgccgt gctctttggc aaattgtatt gttgatgttt gtcgggacgt
     1141 tgttctgggt actggcacaa cagggcggca gtaccatcag cttgtttatc gatcgctttg
     1201 tgaatcgtca ggcattcaat attgaagtac ctacagcact attccagtcg gtgaatgcaa
     1261 ttgcagtgat gctcgctggg gttgtactgg cctggctggc gtcgccagaa agccgcggca
     1321 actcaacatt gcgcgtctgg ctgaagtttg cctttggctt actgctgatg gcttgtggct
     1381 ttatgttgtt ggcatttgat gcccgacatg cagcggctga cggtcaagcg tcaatgggcg
     1441 tgatgatatc cgggttggcg ctaatgggct ttgcagaact ctttattgac ccggtggcga
     1501 ttgcgcaaat cacgcgtctg aaaatgtctg gcgtattaac cggtatttat atgctggcaa
     1561 caggcgcggt cgccaactgg ctggcaggcg tcgtggcaca gcagacgaca gagtcgcaaa
     1621 ttagcggtat ggtaattgca gcttaccagc gattcttttc tcagatggga gagtggacgt
     1681 tggcttgtgt cgcgatcatc gtcgtattgg cctttgctac ccgttttctg tttagcacgc
     1741 cgacgaatat gatacaggag agcaacgatt aactgtcgta aaaacaattg gttatgaata
     1801 aattaacttt c
//
LOCUS       DAZOZD010000456         1803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOZD010000456 DAZOZD010000000
VERSION     DAZOZD010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.464"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..246
                     /locus_tag="ACHUPB_004618"
     CDS             <1..246
                     /locus_tag="ACHUPB_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0761199.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(247..1677)
                     /locus_tag="ACHUPB_004619"
                     /pseudo
     CDS             complement(247..1677)
                     /locus_tag="ACHUPB_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
BASE COUNT          542 a          454 c          391 g          416 t
ORIGIN      
        1 gatgttgatg ctgaactgga tgccgttttg atgggtgctg gtatcgggta tctgctctat
       61 gaacagatca aggagtacct tgataccggt cgtttagaat gtgtactgga agactggatc
      121 acagagcgtc caggcttcca gatctattac ccaaaccgcc agtatatgtc ctgcggcttg
      181 cgcgcatttc tcgattatgt aaaaacgggt cagatctgcc agagtcagcg tcaccgacca
      241 caataattat ttactattcg ctttacgcgc cagcagcggg aatatcagtc ctaatcctac
      301 cagcacaaac ggcgttgcaa cattcagcgc caactggaag gtccactctg cagtgaaggc
      361 ttccattttc gggaagatcc ccgtcaaaca ggcaaaggcg gtaaaggcaa aacaccaaat
      421 cccgacggtc attgccagag gcttattacg aataaagaca tattctggtt tatatttctg
      481 cgccaggcga acgactgcaa taaatgcaac aaatacccat aaataacgca gcggcatcac
      541 taccgaatta aggttcaaca gccatttata gagattgttc atatcaccaa tgccgagagt
      601 cggcagcata atcagaatcg ccaccagtac cagggtcaga aaatagccat taacgggcgt
      661 accagaagcg ttggtacgac ataaactggc tggaatatat ttgctgtcag catcacctaa
      721 tagcacttta agcggggcat caatcgagaa taccagcgcc gctacttgtc ccagggtatt
      781 cgcaatggcg taaatcacca ttaaagtatt acccatgttg taatactcgc ccagcttctg
      841 aaaggcgtaa tactgaccgt tggtcattaa gtcatccggg atattacgcg aatcaaacat
      901 catccccatc gccagcgagc ccagaatggc acaaaccgca accatcaccg ccaggcataa
      961 catccctttt ggaaattctt ttcctgggtt gcgcgtttga ttaacgtaag gagaaatctt
     1021 ctctgctccg ccaaccgcga aaaccagcat tgaaatagtg gtaatgtagg taaagtcgat
     1081 atgaggaatg aacgtttccc aggtaatgtt tgtggtcgca atatgcactt cagtaattgc
     1141 aggcgcggtt accgccatcg ccacatacag gagcgacata acaaacatag caatccctgc
     1201 cacagaaccg acgattttca gcgatttcat accgcgtgaa gcaacccaca taaagaagat
     1261 aaacagcacc agcgttaacc cctgtaacgc tacgactgaa tattctttga ttagcgaacc
     1321 gtcgcctttc atcgcccaac cgagcgcaat cagaattgcc tagggttttt gtgccagata
     1381 gggaatatgc accacccagt aggtccacgc ggcgagataa gccagtccgg gtcccatcgt
     1441 gtgtttaatc caggtactga ccccgccctt cccatctttg aaggtcgagc ctaactgacc
     1501 aacaattagc gcataaggtg tgaaatagag cgcaaagata aacacccatg aaaaaacaac
     1561 caccagcccc tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa
     1621 agacatcagc gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga
     1681 caaatccttc gccaaaatta taggggcatc tgtaaaacga gggattatgc gcggcaaata
     1741 agaagtgcgg aaatagcgaa atgcaaaatt ataacctgct acacatttta tttttaaacg
     1801 aat
//
LOCUS       DAZOZD010000457         1769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOZD010000457 DAZOZD010000000
VERSION     DAZOZD010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.465"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..74
                     /locus_tag="ACHUPB_004620"
     CDS             <1..74
                     /locus_tag="ACHUPB_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0761200.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
     gene            74..421
                     /gene="tnpB"
                     /locus_tag="ACHUPB_004621"
     CDS             74..421
                     /gene="tnpB"
                     /locus_tag="ACHUPB_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0761201.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            441..1595
                     /locus_tag="ACHUPB_004622"
                     /pseudo
     CDS             441..1595
                     /locus_tag="ACHUPB_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1599..1769
                     /locus_tag="ACHUPB_004623"
                     /pseudo
     CDS             1599..1769
                     /locus_tag="ACHUPB_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          422 a          475 c          503 g          369 t
ORIGIN      
        1 tcaggcttaa aggtaaactg actccggcgt tattacagat acttatccgc gaaataaaag
       61 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      121 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      181 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      241 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      301 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      361 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      421 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      481 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      541 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      601 tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      661 tacaggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
      721 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccat aaaccgttac
      781 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
      841 gtggtggcga actcagttct ctgggatgtg atgtgttaga gcagctggag cttatcagca
      901 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
      961 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1021 tggcccatgt tgtcgctgga aaatatgcag actatctgcc gttataccgc cagtcagaaa
     1081 tataccgtcg tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg
     1141 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
     1201 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
     1261 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa atgcctccgg
     1321 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1381 gttacagcgg agtgcttcag gccgatgctt acggtagtta ccgggcgttg tacgaatccg
     1441 gcagaataac ggaagcccag cagcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     1501 ggggatgttc agcagaacag cgtctggcgg caagaaaagc cagagccgcg ccactgatgc
     1561 agtcactgta tgactggata cagcaacaga tgaaaattca ttcgctgaaa atggaatgtc
     1621 tccacggaga acactattat ccatcgggaa atagtgcggg caacagtgtt tagttatatc
     1681 gagtgcgatt acaatcgctg gcgacggcac cgttggtgtg gtggtctcag tccggaacaa
     1741 tttgaaaacc agagcctcac ttacgactg
//
LOCUS       DAZOZD010000458         1756 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOZD010000458 DAZOZD010000000
VERSION     DAZOZD010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.466"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..689)
                     /gene="emrY"
                     /locus_tag="ACHUPB_004624"
     CDS             complement(<1..689)
                     /gene="emrY"
                     /locus_tag="ACHUPB_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0761202.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDL"
     gene            complement(689..>1756)
                     /gene="emrK"
                     /locus_tag="ACHUPB_004625"
     CDS             complement(689..>1756)
                     /gene="emrK"
                     /locus_tag="ACHUPB_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0761203.1"
                     /translation="AYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYV
                     RQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLE
                     DYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQV
                     VEAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNAN
                     FKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKI
                     VQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPELASTVTSMPAYTSKAL
                     VIDTSPIEKEISNIISHNGQL"
BASE COUNT          487 a          401 c          354 g          514 t
ORIGIN      
        1 agatcgcgcc ctttatcaag cataatttgc aagccaccaa caccgagcac taacagggtc
       61 agtcctggta gattcatttt gaccggtgaa gtctcagttt ctcttccttt aagtaaggtt
      121 aagcataatg tcaggacgat aatccccata gggacattga ttaaaaatat ccaaccccag
      181 ctaaagttat cacaaatata accgcccaat atcggcccac atatcggagc gataatcacg
      241 gtcattgacc ataatgccag agcaaatgtt cttttttctg gcggataatt ccttaataat
      301 aaactctgtg acagtggaat taacggcccc gccattaacc cctgaacgac tctaaaaaat
      361 atcagcacat caagattggt cgataggcta cacattaatg aagacagcga aaaaaaggtt
      421 actgaaagta aaaataatct taattcgcct attctttgtg ccaacctgcc agtaacaggg
      481 atcgcaatgg catttgctac accaaacgag gtgataaccc aggtgccttc gtctgttgat
      541 gctcccagaa agccagatat tgtcggtatt gcgacgttag aaatagtgga atccaacatt
      601 tgcataaatg tcgctaatga caatgcaata gtgacgcacc ataacgtccc accggttaat
      661 ggtgccggag ttgatttagt gattgccatt aaagttgtcc attatgcgaa ataatgttgc
      721 taatttcttt ttctatcgga ctggtatcga taactaaagc cttactggta taagccggca
      781 tggaggtcac ggttgaagcc agctcaggca tctcagcaat gtcttcgtcc ttcgtatcaa
      841 tagttgcagt catcgataaa ccaatgcgca aggggtgttc catgagttct tttggatcaa
      901 gagaaacttc aaccggtaca cgctgaacga ttttgatcca gttccctgtc gcattttgtg
      961 caggtaataa ggagaacgca ttgccggttc ccatattgat ccctgtcacc cgaccatgaa
     1021 acacaacatt ttcaccataa agatcgctga taatattgac tgattgacca atccgtacat
     1081 ccgtgagttg tgtttcttta aagttggcat taacccacat ttgacgtgcc ggtacgacag
     1141 ccattaacga ttgtccggga ctcactgttt cgccgacctg aacacttcgc tgggcaatat
     1201 agccagtaac cggactcctt atatccgtac gtttaagcgc caaccaggct tctttggttg
     1261 catccgccgc ttcaactact tgtggctgac ggtttaatgg tgtgttcatt actaaagctt
     1321 tattcgcttt ataagcctgg atagcggcat tcagtgccgc tttgctactt attaacgtat
     1381 ctttggtatg ctccagcgtt tcttttgaaa taaccccctg cttcgctaaa ggcacacgac
     1441 ggttataatc ttctaaggat tgttgatact gaatacgtgc tgaagcgact tcggcactgt
     1501 attgtttatc ctgtaagtac agtttattcg tttgccgaac aatatttgcc agattatttt
     1561 tagctttatt gagtgcgata gtggcatcag ttttatccag tgaaactaaa atgtcaccct
     1621 gtcgaacgta gttcgtatct ttatgattaa ctacagtgac actacctgag acttgtgcag
     1681 aaattggatc tgcattcccc gtgacatagg cgtcatctgt actaatcatg tcttctaatt
     1741 ccattgacca ataggc
//
LOCUS       DAZOZD010000459         1717 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOZD010000459 DAZOZD010000000
VERSION     DAZOZD010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.467"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..676
                     /gene="fimH"
                     /locus_tag="ACHUPB_004626"
     CDS             <1..676
                     /gene="fimH"
                     /locus_tag="ACHUPB_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0761204.1"
                     /translation="DSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRP
                     FINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGD
                     IGASLFSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQD
                     LGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGY
                     LRVDYQ"
     gene            687..1202
                     /gene="sfmF"
                     /locus_tag="ACHUPB_004627"
     CDS             687..1202
                     /gene="sfmF"
                     /locus_tag="ACHUPB_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR0761205.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1205..>1717)
                     /gene="fimZ"
                     /locus_tag="ACHUPB_004628"
     CDS             complement(1205..>1717)
                     /gene="fimZ"
                     /locus_tag="ACHUPB_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR0761206.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          443 a          361 c          415 g          498 t
ORIGIN      
        1 tgatagtgtg atggatattc agttcccccc gcaaaattat attcggatgg gaacagatcc
       61 taacgtttcg caaaaccttc cgttcggggt gatggattct cgtttgatat ttcgtttaaa
      121 ggttattcgt ccctttatta acatggtgga gatccccaga caggtgatgt ttaccgtgta
      181 tgtgacatca acgccttacg atccgttggt tacacctgtt tataccatta gttttggtgg
      241 ccgggttgaa gtgccgcaaa actgcgaatt aaatgccggg cagattgttg aatttgattt
      301 tggtgatatc ggcgcatcgt tatttagtgc ggcagggccg gggaatcgac cagctggcgt
      361 catgccgcaa accaagagca ttgcgatcaa atgtacgaat gttgctgcgc aggcttattt
      421 aacaatgcgt ctggaagcca gtgccgtttc tggtcaggcg atggtgtcgg acaatcagga
      481 tttaggtttt attgtcgccg atcagaacga tacgccgatc acgcctaacg atctcaatag
      541 cgttattcct ttccgtctgg atgcagctgc ggcagccaat gtcacacttc gcgcctggcc
      601 tatcagtatt accggtcaaa aaccgaccga agggccgttt agcgcgctgg ggtatttacg
      661 cgtcgattat caatgaggta cggagaatga gaagagtact ctttagctgt ttctgcgggt
      721 tactgtggag ttccagtgga tgggcagctg accctttggg aacgattaat atcaatttgc
      781 acggtaacgt tgttgatttc tcctgtaccg taaacactgc ggatattgat aagacggtag
      841 atttaggcag atggcctacg acacaattac tgaacgctgg cgataccacg gcactcgtcc
      901 cttttagcct gcggctggag ggatgtcctc cgggttccgt tgcgatttta tttacgggaa
      961 cgccggcatc cgataccaac ctgctggctc tggatgatcc cgcaatggca caaaccgtcg
     1021 ccatcgaatt acgtaatagc gatcgctccc ggcttgcact gggggaggcg agcccgactg
     1081 aggaagtaga tgcaaatggc aatgtcacac taaacttttt tgccaattat cgagcgttag
     1141 ccagcggtgt tcagccaggt gtggagaaag cggatgcgat atttatgatc aattataatt
     1201 aatattatat taattcgtat aatttggcgt agtcgataag ctctacaatt gaatgcaaac
     1261 ctagtttgcc ataaatatta gatttatgcg cactaactgt tttattgcta agtaataact
     1321 tatcggcaat ttctttatta gataatccgc taaccagata acgtaatatg gtcacttcac
     1381 gattagatag cacagtgacc gttgaactat tcgtactaca tttattgctt tttatatagt
     1441 tgagcgtttc gctaggaaaa aacgtgtatc cggagaggat catctgaacg ggatgaaaaa
     1501 tatcattctg atcattgcat ttactgacaa aaccgttagc accagcttgt atcgctctgc
     1561 cagcataaaa gcattctgat ttcgatgata aaaataacac tttcactgtg ctctggattt
     1621 gtttgatcct tttcaggaag gtaaaaccgt ctgttccggg caagtctata tccataatga
     1681 ttaaatcaac aggacgggtt cggagataat cgatggt
//
LOCUS       DAZOZD010000460         1710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOZD010000460 DAZOZD010000000
VERSION     DAZOZD010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.468"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..336
                     /locus_tag="ACHUPB_004629"
     CDS             1..336
                     /locus_tag="ACHUPB_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0761207.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(617..748)
                     /locus_tag="ACHUPB_004630"
     CDS             complement(617..748)
                     /locus_tag="ACHUPB_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0761208.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1248..1324)
                     /locus_tag="ACHUPB_004631"
     tRNA            complement(1248..1324)
                     /locus_tag="ACHUPB_004631"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1288..1290),aa:Arg,seq:tct)
     gene            complement(1425..1500)
                     /locus_tag="ACHUPB_004632"
     tRNA            complement(1425..1500)
                     /locus_tag="ACHUPB_004632"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Ile,seq:cat)
     gene            complement(1603..>1710)
                     /locus_tag="ACHUPB_004633"
     CDS             complement(1603..>1710)
                     /locus_tag="ACHUPB_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0761209.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          531 a          435 c          315 g          429 t
ORIGIN      
        1 atgaagtgga ttgtgattga tacagttatc cagccatcat gcggaatatc tttttcagtc
       61 atatggagta aaataaaatt aataatctgg tatcaatcgg atgctttctt acctcctgaa
      121 agtatattta cactgactca cacaggcatc atgctcaata acaaagtgct acctgtaacc
      181 atttacaacg tagtaccatt caataaaaca ttctggaatt caatcaaaaa tagccaggaa
      241 tgccctacaa atacagataa cgtattgaat gaatgcttta ataaccgttg cactctgcaa
      301 atatgtcctt atggactaaa acaacaaagt ccataaggag tttactcaca tctgacaaaa
      361 tcaatataaa cagcccctcc ggagaggggc tggagagtgg cgctatgtgc cattgcatgg
      421 tgccgggtgc ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac
      481 ctactcgctg attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac
      541 tccccgcaaa ccagacaaca gtcaaccgcc tgaattgtga agtatttaaa aatttctccc
      601 gctaactgat acccggctaa cagtctggcg ttttcttttt cagcaacggg aaagcaacaa
      661 ccaccacacc cgccaccagc acaccgtcag ccagcactga cattatccgg ctgctgcaat
      721 gccattcaca aaaacagtaa gcaatcactt tttaccgtaa caggtgataa tccagatatg
      781 tatctacccc agatgagtaa tccgaagttc atccatacca caggtcctgg ctattctgtt
      841 gtactcctga acaagagcaa ataattctga attagcaacc atgaactcat cgcaaaccct
      901 ctgtatagca tcactattca gaataataac gtctcttccc gaaagacgat caggagtaca
      961 gaacaaaact gtcaaacggc tgaaggcctt tgctcgtgct gcattgacta tatcaatacg
     1021 ctgcctaagg atgaaacacc ccgacgcctc atcaatattc actctaccca caccatatga
     1081 atgataaata tttaatgctg aaaaaaccat tagaccgtat aacaaacact caatcaatac
     1141 ttaacagaac ttttattttt gacaaacata aaatattttc aacaatatcc tgagccaggt
     1201 atatttcagt ataaggctct gccggaagga atctggaaga atgaatatgg cgcgctgtac
     1261 tggattcgaa ccagtgaccg attgcttaga aggcaattgc tctgtccggc tgagctaaca
     1321 acgctgaata ccgataatgg accgccatcg gggacccgcc cccgcaccaa caaccctgtt
     1381 atcgtgtcgt ctgctcttcc tgataagcta atggcggttt gtgatggtgg cccttgctgg
     1441 atttgaacca gcgacttggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1501 gggagcagaa taataacggt ccgtaattaa ttccgcaata aaaaacccgc tcggcggcgg
     1561 gttgtagaaa ctcttctaac gtcaggcata aaacgcccat agttatgacg aatttaccac
     1621 agattccgga aaaatcaacc tagttacctt ttttaactgc cgctcagccc atgcttcttc
     1681 aatatcaaac cgagtcacca gcgcatcata
//
LOCUS       DAZOZD010000461         1693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOZD010000461 DAZOZD010000000
VERSION     DAZOZD010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.469"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..799
                     /locus_tag="ACHUPB_004634"
                     /pseudo
     CDS             1..799
                     /locus_tag="ACHUPB_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="ACHUPB_004635"
                     /pseudo
     CDS             914..1255
                     /locus_tag="ACHUPB_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1693
                     /locus_tag="ACHUPB_004636"
                     /pseudo
     CDS             1213..1693
                     /locus_tag="ACHUPB_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          424 a          409 c          499 g          361 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggccagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat
     1261 cccgacaaca gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag
     1321 gacggtgcct gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga
     1381 cgtattgtca gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa
     1441 agtggtggta catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg
     1501 ttatccaggc gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag
     1561 aaacacaaaa tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg
     1621 ttaagctgga aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg
     1681 atggcatgac agc
//
LOCUS       DAZOZD010000462         1690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOZD010000462 DAZOZD010000000
VERSION     DAZOZD010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.470"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(51..1448)
                     /gene="ccp"
                     /locus_tag="ACHUPB_004637"
     CDS             complement(51..1448)
                     /gene="ccp"
                     /locus_tag="ACHUPB_004637"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0761210.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          453 a          444 c          349 g          444 t
ORIGIN      
        1 ttatcgtaat aaaaatcgcc caaacattcc tgttcgggcg atcaaattaa ttattgttta
       61 tcctgcatat acggcgtgta caccccgttc agactgtgca ggaaagctac gatatcatcc
      121 acatcctcct gcggcagctc tttgcctacc tgatagcgca gcatcagttt caccgccccg
      181 tccagcgtcg gcacgtcacc gcgatggaag tacggtgccg ttaaagcaac gttacgtaaa
      241 ccgggtactt tctgacgcaa tttatcacgc tcttctttag taacattcat acgaccaata
      301 tccgccgccg taatttcccc aaagttaaag tcttttttca gccccaacgg ttcaaaggaa
      361 cgtccaccga gaataatacc accatgacaa gttgcacatt tattatcttt aaataattga
      421 tagccttttt tctgttgcgc cgtcagcgca ttttcatctc cacgcaacca tttatcaaat
      481 ggggaatccg gcgtaattaa tgttttctca aattcagcaa tggcatcagt aatattttcg
      541 ccactgaaac cttgcggata gacgccgagg aactgcgctt taagctgcgg atctttttcc
      601 agcttagcaa taatttcgtc ccaagatttc gacgccattt caatcgggtt caacggcggt
      661 ccaccagcct gatcctgcaa tgttgccgca cgaccatccc agaactgctc aacgttaaat
      721 actgagttaa ataccgtcgg cgcgttaatc ggcccaactg cgccaccaac accaatcgat
      781 gtttttctgc catcgacgcc ccccgcattc aacgcatggc aatgagcgca tgaaatggtg
      841 ctatcagccg ataaacgggg atcgtgatac agcgcaaagc ccaacgccac tttttgcgca
      901 tcggtaggca gtttttgcgg gatgggctgc accggttcat tgcgatgctc cggagcagta
      961 tcattgctgg cgtaatattc cgcgcgctgt tttgcaatcc aggccagtat ttccgcccgc
     1021 tcttcatcac tcaccttacc cgcccagtga agcgcggtat aacgcgttgg cggcatagtt
     1081 tcatactgca tcacccattc aatcttattc agatcgcttt gcgaaacggg tttatcagcc
     1141 agcagtgccg cacgcacggc ctcgaggtta aaagatttat atccaagctt aatgtcgtaa
     1201 tccatcaact gtttcgcgcc aggaatataa taataggcgg gtaattctgc cgaaggcgtg
     1261 tggcaatagt cgcatccttt ttcgcggaga aaacctaaaa ccttattatt ttcactgaca
     1321 gcggatgcct gaacatcggc ctgtttactg cgtttattat cgtggtacca cacataacca
     1381 gataacccta aatagcaaat cgcgacgcca gccaggccga tcgcggtaat acgtgagacc
     1441 attttcatta ttcttccctc acggtggtga gttattgtga cccagaaaat tttccagaat
     1501 gctgcaaacg tctctgcgag acaaagccat cataaaaaac agtgagatac gctttttgat
     1561 aggaatcaat caattacatg aatataaact atcaataaga tagcctgtgc ctattaccca
     1621 gatcaggaat ggcatatcat gattgcggcg agagcaggat tgaatgttct gaaaatgaaa
     1681 aacaacacga
//
LOCUS       DAZOZD010000463         1676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOZD010000463 DAZOZD010000000
VERSION     DAZOZD010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.471"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHUPB_004638"
     CDS             complement(<1..889)
                     /locus_tag="ACHUPB_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0761211.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="ACHUPB_004639"
     CDS             complement(989..1345)
                     /locus_tag="ACHUPB_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0761212.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="ACHUPB_004640"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUPB_004640"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAZOZD010000464         1671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOZD010000464 DAZOZD010000000
VERSION     DAZOZD010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.472"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUPB_004641"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUPB_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR0761213.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..>1671
                     /gene="istB"
                     /locus_tag="ACHUPB_004642"
     CDS             1253..>1671
                     /gene="istB"
                     /locus_tag="ACHUPB_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR0761214.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATL"
BASE COUNT          411 a          420 c          475 g          365 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac t
//
LOCUS       DAZOZD010000465         1667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOZD010000465 DAZOZD010000000
VERSION     DAZOZD010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.473"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUPB_004643"
     CDS             complement(<1..105)
                     /locus_tag="ACHUPB_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761215.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGK"
     gene            complement(183..604)
                     /locus_tag="ACHUPB_004644"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="ACHUPB_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            complement(588..815)
                     /locus_tag="ACHUPB_004645"
     CDS             complement(588..815)
                     /locus_tag="ACHUPB_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0761216.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="ACHUPB_004646"
     CDS             892..1299
                     /locus_tag="ACHUPB_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0761217.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="ACHUPB_004647"
     CDS             1503..1658
                     /locus_tag="ACHUPB_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0761218.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          470 a          436 c          344 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaacga
//
LOCUS       DAZOZD010000466         1659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOZD010000466 DAZOZD010000000
VERSION     DAZOZD010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.474"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..505
                     /locus_tag="ACHUPB_004648"
                     /pseudo
     CDS             1..505
                     /locus_tag="ACHUPB_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
     gene            complement(523..1024)
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004649"
                     /pseudo
     CDS             complement(523..1024)
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1032..>1659
                     /locus_tag="ACHUPB_004650"
     CDS             1032..>1659
                     /locus_tag="ACHUPB_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0761219.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQK"
BASE COUNT          460 a          383 c          407 g          409 t
ORIGIN      
        1 ggtagacccg agttttgatc aaagcaaata tgacagcatc gtctggaacc caatcactta
       61 ttatccggtg ccaaaacctt ctacctaggt agggcagaaa gttctggata aaattttgaa
      121 ctataccaac accgaaatga aagaagcgat agcgcagcgt aaaccactgg ttaccaccgc
      181 tgggccgcgt agtctgattt tccgtggggc cattaccggt gtagatacca gcaaagaagg
      241 gctgcaattc tatgaagtgg ttcctgttgc attagtggtt gcggggacgc aaatggctac
      301 aggccaccgt accatggata ctcgactcta ttttgaaggt gagctgattg atgcagcgac
      361 taataaaccg gttatcaaag tcgttcgtca gggcgaaggt aaagacctga ataacgaaag
      421 tacgccaatg gctttcgaaa atattaaaca agttattgat gacatggcga ccgatgccac
      481 catgtttgac gttaacaaaa agtagttcca gacgcgccat cgttagatgg cgctttttat
      541 ccggtgcgcc gtaaaacccc atccttcagg gcggggatat aaggcgcggt tttccacctg
      601 accaggtgtt tgctgttgtt caatgtattg gcggatgacg gatattggca cacctccgca
      661 gctactggca aaatagccag gactccacaa aacgcctttg tagtaatacc tgactgcaat
      721 atctggtcga tctcgtcgca gtaacctacc cgatacgcct ttgaggctgt ttaccagact
      781 ggatatcgcc agtttgggag gttagttaat taacaaatgg acgtgatctg gttcgccatc
      841 catttcaacc agttcagctt caaaataagc acatacattt gaaaagtaag tgcgtagttt
      901 ttctgtcgcg tcaaaaaatc tggcgtcggt atctagtgac aaagaccagg tgaacatgca
      961 tcaggaaaac acaatgcctt ccacgccgga tatcggtttc ttttttcaca gaccaaagta
     1021 tcatttgacc tgtgaaacga ttacaggcat ttaaattcca gttaagacca ggtggtcaac
     1081 aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat cgtgctctgg
     1141 cacgtcagaa tgagaatcat gaggccggta ataaatacat cccttacggg aaaatggctt
     1201 cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat tctccctcac
     1261 agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc ttccggaagc
     1321 gggctgcttt tccccgattc aaaaagcggg gacagaatga tgtattccgc tacccgcagg
     1381 gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc tggatgcgct
     1441 accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt cactgtcagc cagtcctgcg
     1501 gtacgtggta catcagtatt cagacagaaa gtgaagtatc cactccggct cacccttcag
     1561 catcaatggt cgggctggat gctggcgtgg ctaaactcgc cacgctgtca gatggcacag
     1621 tctttgagcc tgtaaacagt tttcagaaaa accagaaga
//
LOCUS       DAZOZD010000467         1659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOZD010000467 DAZOZD010000000
VERSION     DAZOZD010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.475"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..87
                     /locus_tag="ACHUPB_004651"
     CDS             <1..87
                     /locus_tag="ACHUPB_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761220.1"
                     /translation="GISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            293..1576
                     /gene="gltA"
                     /locus_tag="ACHUPB_004652"
     CDS             293..1576
                     /gene="gltA"
                     /locus_tag="ACHUPB_004652"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0761221.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT          424 a          406 c          393 g          436 t
ORIGIN      
        1 ggtatttcac ctaagattaa cttatgtaac agtgtggaag tattgaccaa ttcattcggg
       61 acagttatta gtggtagaca agtttaataa ttcggattgc taagtacttg attcgccatt
      121 tattcgtcat caatggatcc tttacctgca agcgcccaga gctctgtacc caggttttcc
      181 cctctttcac agagcggcga gccaaataac aaacgggtaa agccaggttg atgtgcgaag
      241 gcaaatttaa gttccggcag tcttacgcaa taaggcgcta aggagacctt aaatggctga
      301 tacaaaagca aaactcaccc tcaacgggga cacagctgtt gaactggatg tgctgaaagg
      361 cacgctgggt caagatgtta ttgatatccg tactctcggt tcaaaaggtg tgttcacctt
      421 tgacccaggc ttcacttcaa ccgcatcctg cgaatctaaa attactttta ttgatggtga
      481 tgaaggtatt ttgctgcacc gcggtttccc gatcgatcag ctggcgaccg attctaacta
      541 cctggaagtt tgttacatcc tgctgaatgg tgaaaaaccg actcaggaac agtatgacga
      601 atttaaaact acggtgaccc gtcataccat gatccacgag cagattaccc gtctgttcca
      661 tgctttccgt cgcgactcgc atccaatggc agtcatgtgt ggtattaccg gcgcgctggc
      721 ggcgttctat cacgactcgc tggatgttaa caatcctcgt caccgtgaaa ttgccgcgtt
      781 ccgcctgctg tcgaaaatgc cgaccatggc cgcgatgtgt tacaagtatt ccattggtca
      841 gccatttgtt tatccgcgca acgatctctc ctatgccggt aacttcctga atatgatgtt
      901 ctccacgccg tgcgaaccgt atgaagttaa tccgattctg gaacgtgcta tggaccgtat
      961 tctgatcctg cacgctgacc atgaacagaa cgcctctacc tccaccgtgc gtaccgctgg
     1021 ctcttcgggt gcgaacccgt ttgcctgtat cgcagcaggt attgcttcac tgtggggacc
     1081 tgcgcacggt ggtgctaacg aagcggcgct gaaaatgctg gaagaaatta gctccgttaa
     1141 acacattccg gaatttgttc gtcgtgcgaa agataaaaat gattctttcc gcctgatggg
     1201 cttcggtcac cgcgtgtaca aaaattacga cccgcgcgcc accgtaatgc gtgaaacctg
     1261 ccatgaagtt ctgaaagagc tgggcaccaa agatgacctg ctggaagtgg ctatggagct
     1321 ggaaaacatc gcgctgaacg acccgtactt tatcgagaag aaactgtacc cgaacgtcga
     1381 tttctactct ggtatcatcc tgaaagcgat gggtattccg tcttccatgt tcaccgtcat
     1441 tttcgcaatg gcacgtaccg ttggctggat cgcccactgg agcgaaatgc acagtgacgg
     1501 tatgaagatt gcccgtccgc gtcagctgta tacaggatat gaaaaacgcg actttaaaag
     1561 cgatatcaag cgttaatggt tgattgctaa gttgtaaata ttttaacccg ccgttcaaat
     1621 ggcgggttaa tttttatatg cccaaacaca aaaaattgt
//
LOCUS       DAZOZD010000468         1623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOZD010000468 DAZOZD010000000
VERSION     DAZOZD010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.476"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..923)
                     /gene="corA"
                     /locus_tag="ACHUPB_004653"
     CDS             complement(<1..923)
                     /gene="corA"
                     /locus_tag="ACHUPB_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0761222.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYL"
     gene            complement(1075..1131)
                     /gene="ysgD"
                     /locus_tag="ACHUPB_004654"
     CDS             complement(1075..1131)
                     /gene="ysgD"
                     /locus_tag="ACHUPB_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0761223.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1293..>1623
                     /locus_tag="ACHUPB_004655"
     CDS             1293..>1623
                     /locus_tag="ACHUPB_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761224.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          419 a          418 c          413 g          373 t
ORIGIN      
        1 agatacggtg ccaggcccgc gaggatcata aagataatcg cgcctgggta gccgaaactc
       61 cacttcagtt ctggcataaa ctcaaagttc atcccatagc tggaagcaac gagcgtcggc
      121 ggcaggaata ccacggatac caccgagaag attttgatga tgcggttctg ctcgatgttg
      181 ataaaaccca tcgccgcctg catcaggaag ttcaccttct ggaacaggga ttcgttatgc
      241 ggcagcaggg attcgatatc tcgcaggatt tcacgcgcct gctccagttg cccacccggt
      301 aaacgcgctt tacgcaccag gaagttgagc gcgcgctggg tatccatcag acacaggcga
      361 actttccagc cgatatcttc cagttccgcc agagtggaga gcgcctcgtc gtactcatcg
      421 ccctgatgcc cttccataat cacccggctc aactgctcca ggtcgctata gatattttca
      481 atttcatctg ccaactgttc gattttggtt tcgaacagat ccagcagcag ctcgtaggcg
      541 ttaccgtcta ccatcgactg gctacgggca cgcatacgat acagacgaaa agcgggcagt
      601 tcacgctcac gcagagtaaa cagacgacca tcacggatgg taaatgccac agtggagtta
      661 ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt gaatatgcag gccgtcgtcg
      721 tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt ccgggcgggt tgccaggctc
      781 tggccaagtt cagattgtac gcgcagtcgc tcgtcgtcgt ccggttcgac aagatcaatc
      841 catactgcat ttacaagggg ttgtgactct tcgacttcca gccgggtcag tcggttattt
      901 tccagttgaa atgcgctcag catgaccggg actcccaatg cttaaaatat cggacagttc
      961 ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa cagcctgact cagcgcgacg
     1021 ggaaaaattg aggtcgctga caaccgctaa ggctatcagc aaaaagggat agccttagga
     1081 gttgatcctg gatgacagga tagtgagcca gtatctactg ggtgtgtcca aggcgaatgt
     1141 cctcttagag tgatcgtgcg cgcatgttac gccagcaaaa ttttgccgtc aacacgcaac
     1201 agaacaccac agaacaatag ttgaacttcg cgctttttgg tactcgtcaa ggtcgctaac
     1261 aggataaaaa aatccaacat ccaggaccta taatggcgca tcggctactc attggtaaag
     1321 gaatgatcac cttgaatctc aaacggattt ttctcgccct caccttactt cccttatttg
     1381 ctgttgccgc tgatgattgc gcactctcag atccgatgct gaccgtacag gcgtataccg
     1441 ttaatcctca aacagagcgg gtgaaaatgt actggcaaaa agccaatggc gaagcgtggg
     1501 gaactttaca tgctctgctg gtggatatga atagtcaggg tcaggtgcag atggcgatga
     1561 acggcggcat ctatgatgaa agctatgcgc cgctcggttt gtacatcgaa aacggtcagc
     1621 aga
//
LOCUS       DAZOZD010000469         1618 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOZD010000469 DAZOZD010000000
VERSION     DAZOZD010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.477"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHUPB_004656"
     CDS             complement(<1..202)
                     /locus_tag="ACHUPB_004656"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0761225.1"
                     /translation="MAEPMAQRPICRQNSHRLHDRLGEPQYTDSLHSQGSASSMWMRH
                     VGGHERFRTGDGQLNTQANRYVL"
     gene            complement(162..1618)
                     /locus_tag="ACHUPB_004657"
                     /pseudo
     CDS             complement(162..1618)
                     /locus_tag="ACHUPB_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          364 a          464 c          362 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAZOZD010000470         1606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOZD010000470 DAZOZD010000000
VERSION     DAZOZD010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.478"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            24..575
                     /locus_tag="ACHUPB_004658"
     CDS             24..575
                     /locus_tag="ACHUPB_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0761226.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            635..>1606
                     /locus_tag="ACHUPB_004659"
     CDS             635..>1606
                     /locus_tag="ACHUPB_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0761227.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT          500 a          335 c          318 g          453 t
ORIGIN      
        1 ttatatttat ataaaggaat attatgtctg cttttaaaaa gtcacttctt gttgctggcg
       61 tagcaatgat tctttccaat aacgtatttg ctgacgaagg tcacggcatt gttaaattta
      121 aaggtgaagt tatctctgct ccatgttcaa ttaaaccagg tgacgaagac ttaactgtaa
      181 atctgggtga agtagcagat accgttctga aaagcgacca gaaatctttg gctgagcctt
      241 tcactattca cctgcaagac tgcatgttga gccagggtgg aaccacttat tcaaaagcga
      301 aggttacttt caccacagcg aatactatga cgggtcagac tgacctgctg aaaaacacca
      361 aagaaactga aatcggtggt gccacaggcg ttggcgtacg tattctggat agccagagcg
      421 gcgaagtgac tttaggtact ccggtagtaa ttacattcaa caatactaac agctatcagg
      481 aactgaactt caaagctcgt atggaatccc cgggcaaaga tgctaccccg ggcaacgttt
      541 acgctcaggc tgactataag attgcttacg agtaatcaca ttaggccggg ttcgcccggc
      601 cacatcatta acggattaat gataagtgga acagatgtat aaaaaattaa aattaaccac
      661 aataagcgaa ttgattaaaa atatttattg ttcattatcc gttctcatca ttggttgtgc
      721 gtcagcttat gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc gtgaaaacgt
      781 taacctttcc caatttgaaa ctgatggcca attacccgta ggtaaatact cattaaacgc
      841 tcttattaat aataaaagga cgccaatcca tctggacctg caatgggtat taattgataa
      901 ccaaactgca gtttgtctga ctccagagca attaacatta ttaggcttta ctgatgagat
      961 tattgaagag gctcagcaaa acctgatcga tggttgttac cctatcgaaa aagaaaaaca
     1021 aattacaact tatctcgata aagggaaaat gcaattatcc atatctgcac ctcaggcatg
     1081 gttaaaatac aaagatgcaa actggacgcc tcctgaactt tgggatcatg gtattgctgg
     1141 ggcatttctt gactacaatt tatatgcctc tcattatgca ccacatcagg gcgataattc
     1201 acaaaatata agttcctatg ggcaggctgg ggttaatctt ggggcctggc gcctgcgtac
     1261 tgattaccag tacgatcagt catttaacaa tggcaaaagc caggcgaaca acctggattt
     1321 tccgcgtatt tatttgtttc gcccaatccc agcaattaat gcaaaactaa ctataggtca
     1381 atacgatact gaatcctcta ttttcgactc tttccatttt tctggcgttt cgttgaaaag
     1441 cgatgagaat atgttgccgc ctgatctacg tggttacgca ccgcaaatca cgggtgttgc
     1501 acaaacgaat gcaaaggtca ctgtctcaca gaacaaccgt attatttatc aagaaaatgt
     1561 tcctcctggc ccatttgcta ttactaattt attcaataca ttacag
//
LOCUS       DAZOZD010000471         1588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOZD010000471 DAZOZD010000000
VERSION     DAZOZD010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.479"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            49..813
                     /gene="nfeF"
                     /locus_tag="ACHUPB_004660"
                     /gene_synonym="yqjH"
     CDS             49..813
                     /gene="nfeF"
                     /locus_tag="ACHUPB_004660"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR0761228.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(867..942)
                     /locus_tag="ACHUPB_004661"
     tRNA            complement(867..942)
                     /locus_tag="ACHUPB_004661"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(907..909),aa:Ile,seq:cat)
     gene            1067..1573
                     /gene="mug"
                     /locus_tag="ACHUPB_004662"
     CDS             1067..1573
                     /gene="mug"
                     /locus_tag="ACHUPB_004662"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR0761229.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          378 a          394 c          443 g          373 t
ORIGIN      
        1 acgaaaatta tttagatata tctgattacc atcacgaagg cgataacaat gaataactcc
       61 ccccgctacc cgcagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac tgtgttacgc
      121 gttgagcgca tcagcgccgg ttttcagcgc attgtcctcg gcggcgaggc gctggacggt
      181 tttacatcgc gcggctttga cgatcacagc aaactcttct ttcctcaacc tgacgctcac
      241 tttgtgccgc caacggtaac ggaagagggc atcgtctggc cggaaggacc acgcccaccg
      301 tcgcgtgact atacgccgct gtatgacgaa ctacgccatg aactggcgat cgatttcttt
      361 attcacgacg gtggggtcgc cagcggctgg gcgatgcagg cgcaaccggg cgataaactt
      421 acggttgcag gtccgcgcgg ttcgctggtg gtgccggaag attacgcgta tcagctgtat
      481 gtctgcgatg aatccggaat gcccgcactg cgccgccgct tggaaacgtt gagcaaactt
      541 gccgttaaac cgcaagttag tgcgctggtt agcgtgcggg ataacgcctg tcaggattat
      601 ctcgcgcacc ttgatggttt taatatcgaa tggctggcac atgatgagca ggcggtagat
      661 gcgcgtctgg cgcagatgca aatccctgcc gatgattact tcatctggat aaccggcgaa
      721 ggtaaagtcg ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg
      781 gtacgtgcag cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac
      841 gatttctcgt cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa
      901 gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa
      961 gtaactccgt gtcgcaatcc agccattacc gtgcgcctgc tgtttttata aacaatgtat
     1021 tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat
     1081 tttggctcca gggttacggg tcgtattttg cggtatcaac cctgggcttt catccgccgg
     1141 gactggtttt ccctttgctc atccagcaaa tcgcttctgg aaggtgacat atcaggccgg
     1201 gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg
     1261 cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct
     1321 gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc
     1381 gattctgggc aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa
     1441 acaaacgctc accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag
     1501 tcgcgtttca ctagagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt
     1561 gcgtgggcga taaaaaacgc caccgaaa
//
LOCUS       DAZOZD010000472         1568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOZD010000472 DAZOZD010000000
VERSION     DAZOZD010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.480"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            133..537
                     /gene="stpA"
                     /locus_tag="ACHUPB_004663"
     CDS             133..537
                     /gene="stpA"
                     /locus_tag="ACHUPB_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0761230.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(584..1108)
                     /gene="ygaP"
                     /locus_tag="ACHUPB_004664"
     CDS             complement(584..1108)
                     /gene="ygaP"
                     /locus_tag="ACHUPB_004664"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0761231.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1118..1417)
                     /locus_tag="ACHUPB_004665"
     CDS             complement(1118..1417)
                     /locus_tag="ACHUPB_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0761232.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          443 a          361 c          361 g          403 t
ORIGIN      
        1 cagaaaataa cagctgaaat aatctcgcgc aagactgtaa atagattaaa ttttgtggaa
       61 atataataag tgatcgctta cactacgcga cgaaatactt ttttgttttg gcgttaaaag
      121 gttttcttta ttatgtccgt aatgttacaa agtttaaata acattcgcac cctccgtgcg
      181 atggctcgcg aattctccat tgacgttctt gaagaaatgc tcgaaaaatt cagggttgtc
      241 actaaagaaa gacgtgaaga agaagaacag cagcagcgtg aactggctga gcgtcaggaa
      301 aaaattagca cctggctgga gctgatgaag gctgacggaa ttaacccgga agagttattg
      361 ggtaatagct ctgctgctgc accacgcgct ggtaaaaaac gccagccgcg tccggcgaaa
      421 tataaattca ccgatgttaa cggtgaaact aaaacctgga ccggtcaggg ccgtacaccg
      481 aagccaattg ctcaggcgct ggcagaaggt aaatctctcg acgatttcct gatctaatta
      541 ctgaggccgg atgtcgctgc tagggcgcgt ccggcgtcgc ttctcaagct cgttggttcc
      601 acggcatctt atctaacaac cttgccattc cacaaaaacc gctgattcct gcaaacagta
      661 acccggcacc aacaaagcca cttaataaga agaaaccgct atttacggta taacccagta
      721 caacgccgat taatattaaa ccacccgcag cgatctgcac ctggcgcatt aacggcaagg
      781 gttgagattt atttaccgct actggcaatc cagctctttt ccagccatca atcccatctt
      841 cgagtaaaaa tatttctgcg ggggcggcaa tcgctgctaa tttatcggcg ttattactgg
      901 tgcgtttacc tgcctggcag tggaatataa tttgctcgcg acgtaattta ggtggaagac
      961 ctgactgttc cagcacggat aatggagcca gatctgcttc aggaatatgt tcacgaagat
     1021 attcatcagc gtcacgaata tcgattaact ttgcgccgcg tgcgattaat tcttgtgcat
     1081 catgcggcga aatggttgtc aaagccatag tgactcctta cggacaatag acatttttca
     1141 gggtggcgat aatggcattt accgcctcat ttttaatgga atatagaatg cgttgggcat
     1201 cccgttggct gtcgataagc ccttcgtccc gcatacgagc gagatgctgt gatgtcgcag
     1261 aggcactcag tccggtaatg cgcgtcagct ctcccgcgct ggtgccgggg gaaccgctaa
     1321 gcatgcacag aatcagcaat cgtttaggat ggctcattgc tttcaataag gccgctgcct
     1381 gttcggcact ggcctgtaat tgcgcgagtt cagtcatatt tatttaagta ttttctaaat
     1441 taagtaagct ctaaactaaa aatgcaacat ataccagcct cagcagcgta aatgagagta
     1501 aaagcgtaag ctgaaactgg caggctccgc taaaattact acgcttaaga gataaaatct
     1561 ctttttaa
//
LOCUS       DAZOZD010000473         1566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOZD010000473 DAZOZD010000000
VERSION     DAZOZD010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.481"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            357..1145
                     /gene="phoH"
                     /locus_tag="ACHUPB_004666"
     CDS             357..1145
                     /gene="phoH"
                     /locus_tag="ACHUPB_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0761233.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT          383 a          353 c          406 g          424 t
ORIGIN      
        1 ataaatgcga gatctgttgt acttattaag tagcagcgga agttcccggc agtgatagtc
       61 agtcactatg gagatcgcgg atggtaacgt cctgtactgg acatgtttta gataatcaac
      121 gcgccaccac tcgcggagtt ttctcctccg gtagtcatct cgtcactttg cattttcaac
      181 ctcatccttt cttttcatgt gttaccgacg ccgtaaacgg tgcgcgtagc cgtttttcgg
      241 cattttatcc aaaagcaaac tatggcttac aaggaagcca accctctgat gttcgtgcgc
      301 ataatcgcgc tgccaacggc gcgtgtgatg aatacaaaca actcaaggtg ctctctatgg
      361 gaagacaaaa agcagtgatc aaagctcgtc gcgaggcaaa acgtgtgctg agacgggatt
      421 cgcgcagcca taagcagcgt gaagaagaat cggtcacctc gcttgtgcag atgggcggcg
      481 tagaagccat tggtatggcc cgcgacagtc gcgatacttc gcccatcctc gcgcgaaatg
      541 aagcgcaatt gcactatctg aaggctattg agagtaagca gctgatattc gccacgggcg
      601 aagccggggg cggaaaaact tggatcagcg cagcaaaagc ggcagaggcc ctgatacata
      661 aggatgtcga caggattatc gtcacccgtc cagttctgca agccgatgaa gatcttggct
      721 tcttacctgg agatatcgca gaaaagtttg ctccctattt tcgcccggtc tacgacgtgc
      781 tggtccggcg cttaggggct tcctttatgc agtactgcct gcgaccggaa atagggaagg
      841 tggaaattgc gccgttcgcc tatatgcgtg gacgtacctt tgaaaatgca gtcgtcattc
      901 ttgacgaggc gcagaatgtg actgccgcgc aaatgaaaat gtttttaacc cgcctcgggg
      961 agaacgtgac ggttatcgtc aacggtgata tcacgcaatg cgatttgcct cgcggcgtgt
     1021 gctccggatt aagtgacgcg ctggaacgtt tcgaagaaga tgaaatggtt gggatcgtcc
     1081 ggttcggtaa agaggactgc gtacgttcgg cactttgcca acgtacgctg catgcctaca
     1141 gttaagtgtg ttatcggtgc agagcccggg cgaaccgggc tttgttttat ttcaacagat
     1201 tttcacttca ggttagcgtt caggggggcg actttgagcg tgttgataag ctgaactgga
     1261 gtctggttta gatactctta ccaggcattt ttcgtcatag catcagacca gtgtaatact
     1321 gccagcgcgg agagtttttc ttccagaagc caaaaaaatg agcatctgcc tatctttttt
     1381 cggccattca acctgtacag gagatctggt gccaattttg cttatgaggt ttaatcaaca
     1441 atgggctata ctttgactga ctgtttaaca ccgaatatat tcctctgatt tattctttag
     1501 ggataatatt ataaatgtaa ggttaatctt aattaattct ttgctaatat tgtgtgttgt
     1561 tatttt
//
LOCUS       DAZOZD010000474         1549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOZD010000474 DAZOZD010000000
VERSION     DAZOZD010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.482"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHUPB_004667"
     CDS             351..476
                     /locus_tag="ACHUPB_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR0761234.1"
                     /translation="MWLPVLFRFLVDSLAMLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHUPB_004668"
     CDS             complement(921..1397)
                     /locus_tag="ACHUPB_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0761235.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          405 c          384 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacacccca catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctatgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZOZD010000475         1536 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOZD010000475 DAZOZD010000000
VERSION     DAZOZD010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.483"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /locus_tag="ACHUPB_004669"
     CDS             complement(<1..1012)
                     /locus_tag="ACHUPB_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR0761236.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGK"
     gene            1267..1513
                     /locus_tag="ACHUPB_004670"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="ACHUPB_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAZOZD010000476         1518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOZD010000476 DAZOZD010000000
VERSION     DAZOZD010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.484"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            60..503
                     /gene="cmtB"
                     /locus_tag="ACHUPB_004671"
     CDS             60..503
                     /gene="cmtB"
                     /locus_tag="ACHUPB_004671"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR0761237.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            531..>1518
                     /gene="cmtA"
                     /locus_tag="ACHUPB_004672"
     CDS             531..>1518
                     /gene="cmtA"
                     /locus_tag="ACHUPB_004672"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR0761238.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          352 a          336 c          388 g          442 t
ORIGIN      
        1 gcacgagtat tttattttta ccgccggacg gtaccctaaa aataaaataa ggataattta
       61 tgcggcttag tgactatttt ccagaatcat caatctcagt tatacattca gcaaaagatt
      121 ggcaggaagc tatcgatttc tcgatggtat cattgctgga taaaaactat atcagcgaga
      181 attacattca ggcaattaaa gattccacca ttaacaatgg cccttattat attctcgcac
      241 caggcgtggc aatgcctcat gcgcgaccgg aatgtggggc gcttaaaacc gggatgtctt
      301 tgacattact tgaacaaggt gtttattttc cggggaatga cgaaccaata aaattactca
      361 tcggcctttc tgctgccgat gccgattcgc acattggcgc tattcaggcg ttaagtgaat
      421 tactgtgcga agaagaaata ctcgaacaac tcttaacagc agcatcagaa aaacaattag
      481 cggacataat cagccgcgga taattactct ctcctctaca ggataatatt atggaaaaca
      541 agtctgctcg tgcaaaggtc caggcttttg ggggcttttt gacggcaatg gtcatcccca
      601 atattggtgc ttttattgcc tggggtttta ttactgcgtt atttattccc accggttggc
      661 tgcctaacga acatttcgcc aaaattgtcg gcccgatgat tacctattta ttgcccgtga
      721 tgattggttc taccggtggt catctggtcg gcggtaaacg cggcgcggtc atgggcggaa
      781 taggtactat tggtgtgatc gtcggcgcag agatcccgat gttccttggc tcaatgatta
      841 tggggccgct cggtgggctg gtcataaaat atgttgataa ggcactggaa aaacgcatac
      901 ctgccggttt tgagatggtt atcaataact tctcattagg tatcgcgggg atgctccttt
      961 gtctgctggg ttttgaagtt atcggcccgg cggtgttaat tgccaatact ctcgtcaaag
     1021 agtgtattga ggcgctggta catgcgggtt atctgcctct gctgtcagtc atcaatgaac
     1081 cggcgaaagt gcttttcctc aataatgcga tcgatcaggg cgtctattat ccgctgggaa
     1141 tgcaacaggc ttcggttaac ggtaaatcca tcttctttat ggtggcgtcc aatcctgggc
     1201 ctggcttagg gctgctgctg gcgtttacct tgtttggtaa agggatgagt aaacgttctg
     1261 cgcccggggc gatgattatt cacttcctcg gtgggatcca cgaactgtat ttcccgtatg
     1321 tgctgatgaa gccgctgacc attattgcca tgattgcggg cggtatgtct ggcacctggg
     1381 tgtttaactt actggacggt ggtctggtgg ctggcccaag tccggggtct atctttgctt
     1441 acctggcact gacgccgaaa ggctcgttcc tggcgacaat tgccggtgtt acggtagcta
     1501 ccctggtgtc ctttgcta
//
LOCUS       DAZOZD010000477         1517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOZD010000477 DAZOZD010000000
VERSION     DAZOZD010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.485"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            117..1160
                     /locus_tag="ACHUPB_004673"
     CDS             117..1160
                     /locus_tag="ACHUPB_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0761239.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          422 a          345 c          330 g          420 t
ORIGIN      
        1 aagcatgaag gggcttcgta agcaatattc attcggtgcc aagacaggac ttgatacaca
       61 gaatctcaac ttttatgctc tcagactata tcaccacatg ttttacaggc tcagaaatga
      121 acgatagatt atgctttgaa gtgcacgata acaagggtta tttcgtattt ccggatacct
      181 ggtttggccc attgctgggg gagtttgaag aagtgcttga tgcatacgac gctgatgaaa
      241 tcagcgagac gagttacatc aacaaactcc gacgccttgc tcaacgggag ccggacttta
      301 ttgatataca cgctcatctg gcatatgcat ttctggaaca gaatgccccg cgtaaggcac
      361 tgaacgcctc tcttaaagga ctggcggcag gtaatcgtat catcccggaa agtttctgtg
      421 gtgaaataat ctggatgcat cccgaaaacc ggccttatct gcgcgcgctt tatgcagcaa
      481 ttctcgcaaa tgtgcactta cagcgccatc aggatgcggt tatgctcact gataaaatac
      541 tggcatacaa tcctgaagat aatcaggggg ctcgctggtt gttgggttca gagctgttac
      601 gcaccggcga tcatgagcga gccttcagtg tactgaaaga gcatgctgat gaattttcac
      661 cttactggta tgaactcggt ctcctgcatt ttctgaacgg ggaacatgtt aaggccgcca
      721 cagcttttcg ccatggcttt gccacaaaca cttacatcgc agaaatgctg tgcggaaatc
      781 tgcatccatt tccacttgct gtgtggcatg acttctccgg ctctctggat acggcagaag
      841 attactatgc aacatactca ccattatggg gacaatatcc ggaagcattg ctgtttgtaa
      901 actggctcta taaccattcc tccgtgctgc atgaacgatc agaaatcatt aaatgcgccg
      961 aaatgctgat tcaggaagat gatttcgaaa tatgcgaaag tattttaaga cagcaggagc
     1021 acctgtggaa aagaattgac aagacacttt ctgaggaaat tgtacagaaa tgcagaaata
     1081 tgaacggtga atatatctgg ccctggatat tgcccttttc cgcagcaggg atgaaacatt
     1141 caagcatcca gtatcagtag atattgcatt agtgtaactt taaaacaagt aataatttat
     1201 tatccaataa taacactgtg ttccttcccc aggtgattac cctggggtta ttttctgaaa
     1261 gtttcctttg caaaaggata gcgttgtaaa ttactttaac gaattactcc atgaactcat
     1321 taaagttctt tcgtaatact ttatcgtcaa acacgttata gctggcatca aaaaccacag
     1381 atttaccctg ccagcgggta atcccctcct ctggcgtttt cggttgaact ggtatccccg
     1441 tggatttgcc cctatatttc cagacatctg ttatcactta acccattaca agcccgctgc
     1501 cgcagatatt cccgtgg
//
LOCUS       DAZOZD010000478         1516 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOZD010000478 DAZOZD010000000
VERSION     DAZOZD010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.486"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>1516)
                     /locus_tag="ACHUPB_004674"
     CDS             complement(<1..>1516)
                     /locus_tag="ACHUPB_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761240.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAZOZD010000479         1470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOZD010000479 DAZOZD010000000
VERSION     DAZOZD010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.487"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHUPB_004675"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="ACHUPB_004675"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0761241.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1458)
                     /gene="cadB"
                     /locus_tag="ACHUPB_004676"
                     /pseudo
     CDS             complement(1090..1458)
                     /gene="cadB"
                     /locus_tag="ACHUPB_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          410 a          323 c          367 g          370 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg cagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg attatcaggg cagtcatttt
     1441 cactgcagcc agcagcagta atgccgatca
//
LOCUS       DAZOZD010000480         1461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOZD010000480 DAZOZD010000000
VERSION     DAZOZD010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.488"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="ACHUPB_004677"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="ACHUPB_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0761242.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="ACHUPB_004678"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="ACHUPB_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0761243.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          446 a          276 c          337 g          402 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt g
//
LOCUS       DAZOZD010000481         1458 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOZD010000481 DAZOZD010000000
VERSION     DAZOZD010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.489"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(233..1396)
                     /locus_tag="ACHUPB_004679"
     CDS             complement(233..1396)
                     /locus_tag="ACHUPB_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0761244.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          375 a          396 c          337 g          350 t
ORIGIN      
        1 tttgattaga gccgtcgcag gaggaacaac tcatatcatc attgcgatct cgacgatgta
       61 tagtctcatc ctgcggcaga acaagacgga taaaaaatcc aatgagagca cactcatcca
      121 gaacttatta acataatgaa tattaaagaa aaatcatcgc agccaatgaa gtaatatgca
      181 aaaataatta gcccttgcag ggagcaaagt tccataatct cccccttccc cctcaatgat
      241 ccaaatacaa ataatgcatc caactggtca ttcttagcag caccttccgc atcaccgaaa
      301 catggctgaa atggtcggag taaactgcta cctgggcgta aatgccgtcg ggtaaggcat
      361 cgatatcatc gttagggtcc agttcaatgg ttcccagcac gccgtccgtg ccgggaacga
      421 ccgttaatga ttgcaatacc ccctgcgcct gataagaacc gcctggcacg acaggtaaaa
      481 tactagtcaa tttgccgtgg aacacctgcc caggtagcgc gttaaacacc acttccgcat
      541 catcgccagg tttcagacgt aacagcgagt tttgccgaaa ttgggcgaca atttgccgtt
      601 tttgctcggg gatgaagacc atcaccggac gcagcggcaa ggcagctgcg tatgtacctg
      661 ggcggatcag tacctgagta acgtagccat tgctcggcgc gcgaatgaca gtctgctcaa
      721 ggttatattt tgcttcagta agttgcgctc ttaagctcac aatctgcgat tgctcgccgt
      781 taaccatact gtcgagctgg ctctggatct gcgcctgctc cgccaccgag cctttcacca
      841 gcgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat gtcacgttcc gagaacggat
      901 tcaccgccgc ctggctgcct ttcaggtaac gttgataatt tttaaacaga cggtcgcgct
      961 ccgctgaaac ctgggtggtg ttggcctgcg cttcagtgag ctgcgcacgc agcgtcttta
     1021 tattatgcgt cgccgtcatc aggtcagcct gaagtctgtc aactcgcgcc tggtaacgaa
     1081 ccgggtcgag cttaaaaagc acctcgccct tttgaataag ctgattattc ttgtcagtga
     1141 cttcagtaac aattcccgtc acctgtggcg tgatagggat cgctatcact gccttttgcg
     1201 cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat caaaccactc accagaaaca
     1261 cgcctcccag cgccgccgtc gccagcgtcc actgattcac cggaatacga aagattttaa
     1321 agaccgccca cgccagctgc aaaatggcat ctgaatcgat cgcagtttgg cattcgaatc
     1381 gatcatgatt tggcatccat ctccgatcac agtttggcat cacaatcgat cacgatttgg
     1441 catacttccg atcattga
//
LOCUS       DAZOZD010000482         1445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOZD010000482 DAZOZD010000000
VERSION     DAZOZD010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.490"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            292..726
                     /locus_tag="ACHUPB_004680"
     CDS             292..726
                     /locus_tag="ACHUPB_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR0761245.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            821..1087
                     /locus_tag="ACHUPB_004681"
     CDS             821..1087
                     /locus_tag="ACHUPB_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761246.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            1264..>1445
                     /locus_tag="ACHUPB_004682"
     CDS             1264..>1445
                     /locus_tag="ACHUPB_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR0761247.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVAT"
BASE COUNT          332 a          396 c          407 g          310 t
ORIGIN      
        1 cccctgcggc ctgtttcccc gctgtggact ttccgggggc tgaagcggcc acaacacgtt
       61 tcagccgtcc aggggagaga tgtaccagcc acaaaacttt ttgccgcaag cgtcaaaaac
      121 ttttgcgtct gcccccggac aactgccccg tgccgtggca gtcgctgccc catcggaaag
      181 gtccactgcg gggagaaacc ccgaagaagt ggacggcaga aggggctttc gcccctccgg
      241 gggagcaacc ggtcggcccg gcgacctgat tcagcagaag gagacgtttt catgacagtc
      301 agcagcacga tttccgtttt ttgccgcgac ggggtgttcc gtaccgttta ctgccacctg
      361 cacggtgagc cgacctggaa cggtcgcatc ctgcataccc actatgccac cggtcagcag
      421 gccgaagccc tggttgaaca cggtgatatc cgttgcctcg gtccccgttg cgacaaaccc
      481 gccgggcata cgcttcagaa cccggtggac ggtgtgacgg cttattacgg acgtgacagt
      541 ggtttccgga tggacagtga ggcgcgtgag taccgttctt tcagggaggc catcgccacg
      601 gaaagcactg aagaggtgcg cttccattat gtgttcatcg acggctactg gaaggtgatg
      661 taccgcacgc cggaaggctg gaagatgaaa gcgctcgcgc tggcactgcg tcgctgtccg
      721 gaatgaaaaa aaaagcaggg cgataaaccc tgcctttctc cgctggcgct tccccgtacg
      781 gaagtccggc atctcattca ctaactatgg agattatgcc atgagaccat caattatctt
      841 cgcaaccgcc gagtatgtaa agcgtctgcg agaagagtgc ctgcgggaga acaaacccct
      901 gcaccgccat acccgcttca gacgccagga actggcaccg gatgagatta acccggacgt
      961 cctggcgatg ggcggtcata tcgccagacg ctgcagtgag cagaagcggg tacgtatccc
     1021 cgccatgaaa gtcagcgaat ggggccacct gctccgcgcg cttgaaattg aacgggtctg
     1081 tcactgatta acccctgccg tctgcctgtg caggtggcag gatgtgttca ggtactttac
     1141 caggaggaac cgtgagcaag atgaaaaaca tcacgacgtc tgtgttgcat ggtgtctcca
     1201 caaaaaacag aaaagggaaa cagattctct ttcattcagg gcccgggagg gctggagata
     1261 acaatgaagt cttgttttga tactgacaaa gcttttcata tgtcttttga tgaagtcact
     1321 ttcaacgctg acagaattcg ctcaaaactg gaactattat ccgaagaact aatgccccgt
     1381 tgtggtcttg tttatgagct atatcagcga cgtttgagtg ctgctattga taattttgtg
     1441 gccac
//
LOCUS       DAZOZD010000483         1431 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOZD010000483 DAZOZD010000000
VERSION     DAZOZD010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.491"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPB_004683"
     CDS             <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPB_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0761248.1"
                     /translation="SQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLE
                     IAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDV
                     PLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPB_004684"
     CDS             464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHUPB_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0761249.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAZOZD010000484         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOZD010000484 DAZOZD010000000
VERSION     DAZOZD010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.492"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUPB_004685"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUPB_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0761250.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUPB_004686"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUPB_004686"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0761251.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="ACHUPB_004687"
     CDS             complement(822..>1428)
                     /locus_tag="ACHUPB_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0761252.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAZOZD010000485         1420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOZD010000485 DAZOZD010000000
VERSION     DAZOZD010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.493"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHUPB_004688"
     CDS             <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHUPB_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR0761253.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          421 c          436 g          225 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg
     1261 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
     1321 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
     1381 accgcaccgt gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAZOZD010000486         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOZD010000486 DAZOZD010000000
VERSION     DAZOZD010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.494"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUPB_004689"
     CDS             <1..459
                     /locus_tag="ACHUPB_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0761254.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUPB_004690"
     CDS             477..1115
                     /locus_tag="ACHUPB_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0761255.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZOZD010000487         1376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOZD010000487 DAZOZD010000000
VERSION     DAZOZD010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.495"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..983
                     /locus_tag="ACHUPB_004691"
     CDS             <1..983
                     /locus_tag="ACHUPB_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0761256.1"
                     /translation="VVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="ACHUPB_004692"
     CDS             998..>1376
                     /locus_tag="ACHUPB_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0761257.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAZOZD010000488         1370 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOZD010000488 DAZOZD010000000
VERSION     DAZOZD010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.496"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..374)
                     /locus_tag="ACHUPB_004693"
     CDS             complement(<1..374)
                     /locus_tag="ACHUPB_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR0761258.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYI"
     gene            complement(595..1326)
                     /gene="bluR"
                     /locus_tag="ACHUPB_004694"
     CDS             complement(595..1326)
                     /gene="bluR"
                     /locus_tag="ACHUPB_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR0761259.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          356 a          311 c          305 g          398 t
ORIGIN      
        1 tcaatatata aaagcgtatt atcaatggcg tagtaactgc gtttgttatg attaacatca
       61 gtaacccaac ggaaaacgcc cgcgcctgcc agtgttgaac agtattcccg aaatgtagat
      121 tttccgtaaa tatgaagcaa tgcggcctct tttattttag cagggttctt ggtcgtacta
      181 acttttaaca ggttcctggt tcctcttaat aacaaaaccg tgtcatcgtg agtaataatt
      241 ctgatgttat ccgtagccag ataataaatg taatgtgcaa tacggtgatg ttttaattct
      301 gaataaaacc aggagaagtt ttgctctttt ctcacttgct caaacatctt ttgaaaaaca
      361 acgacctgat ccatcaggat aataacctct tgttagttgt gagactgcgt agtgtgcacg
      421 atcggtttta ccacttcaat ctggtctgtc ctttggctgt gatatgtaca gagtgtgata
      481 gagggattac ctgaattctt ccggtgagca ttttgcaacg gaccagctcc ggtacaaatg
      541 ctgttgtggg ttcagattat aacattctgt ctaaggggcg ggataaaggt gaaattaggg
      601 ggcatgaaag atgactttat aaccttgctc accccaatgt tgtaaaagtt cgttttgcct
      661 tctcgttggt gccatgcctg tccagacaat caatgtttgc gtcgggaaca gttccgggtg
      721 cggcgattca atgggatcag caagaacaga aatgtgccat cctgacaatg ataaacgcca
      781 tgcttccagc cacaatcgtg ctctctcttc aacattccac gccatcagaa tggcttcttt
      841 accgggctta cggcgcattt cgaaaagtga ggctgctgca tattcaatta atgcgccgtc
      901 aaacatactg ctcataatgc gggaggtgtt gtgatcaagc acgagacgct ggcgaacagg
      961 aaggtaaaca tgattaatca attgatctac tgggtactct cgacccagtg aaataattct
     1021 cgcgcgtagt ttggcaggat tagccatacg aagaattgac atcatctctt cttgcaggcg
     1081 gttccagtca tcttccgtat cctggctggt ggtttccagt aatgctttaa ctttgcctac
     1141 agggacgcca ttacttatcc aacgcttgat ctcttcgatg cgttgtatgt cttcttcatc
     1201 aaagagtcgg tgtccgcctt cactgcgctg tggttttaac aaaccgtagc ggcgttgcca
     1261 ggcccggaga gtgacaggat taatcccgca acgttcagca acatcaccaa tgctgtaata
     1321 agccacaatt cctccttgcg gtcataaatc tccgccgcct gtacacgacc
//
LOCUS       DAZOZD010000489         1360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOZD010000489 DAZOZD010000000
VERSION     DAZOZD010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.497"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            329..847
                     /locus_tag="ACHUPB_004695"
     CDS             329..847
                     /locus_tag="ACHUPB_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0761260.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1057..>1360
                     /locus_tag="ACHUPB_004696"
     CDS             1057..>1360
                     /locus_tag="ACHUPB_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0761261.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          349 a          323 c          339 g          349 t
ORIGIN      
        1 gaatatctca aaaattatca aaatgataat tcagaaagga taaatacaat atttattcat
       61 gattcagctt tggccatttt atggaggcaa tacggacttt gaacagttgg gtatggttat
      121 ccagatatat attattaagt tattgctgaa taagagattt aatgatttta aaaatagttt
      181 ttacgacaac cgtaaattct agctcggttt gtatgtaaaa aatactgaaa aatgatgcgg
      241 ttccgtgaag tgagctgcat ctgcctgtct gggattaacg gcaggtcatt gtaagtagta
      301 attaaccacg ttaacaagga gtttacaaat gccaacaccg tgttatatct ccatcactgg
      361 tcagacccag ggaaatatta ctgcaggtgc atttaccgct gattctgtcg gcaacatcta
      421 tgtgcagggg cacgaagacg agatgctggt gcaggaattc ctgcataacg ttactgtccc
      481 gaccactcct cagtccggtc agcctgcggg tcagcgtgct cacaagcctt tcatttttac
      541 cgtggcgctg aacaaagcgg ttccgctgat gtacaacgca ctggcctccg gcgaaatgct
      601 gccaaccact gaacttcact ggtggcgtac ctctgtggag ggcaaacagg agcattattt
      661 cactacccgc ctgactgatt cgacgattgt cgatatgaaa ctgcatatgc cgcattgcca
      721 ggacccggcg aaacgcgagt tcacccagtt gctcgaggtt tcactggcct accgcaaaat
      781 tgaatgggaa cacgtcaaat ctggcacctc cggtgccgac gactggcgcg caccactgga
      841 agcataagtt aagccaacag catccggcta tcaggccgga tgctgttttt gtattacgcc
      901 gtagaaagaa aggttgtcag taaggggtta cgttcacggg agatggcgga tgccggaaac
      961 ttcggcggta gcgtgcctga acgcaagcgt aacactgtac tgtcacagcc gctttaacag
     1021 cgaactgtct gtttttcctt aaccccggag gttgctatgt caaccggatt acgtttcacg
     1081 ctggaagtgg acggcctgcc accggatgct tttgcggtgg tctcctttca tctgaaccag
     1141 tcactctctt cgctcttttc cctcgagctc tctctggtca gccagcagtt tctctccctt
     1201 gaatttccgc aggtactgga caaaatggcc tacctgacga tatggcaggg cgatgacgta
     1261 cagcgccggg tgaaaggtgt ggtgacctgg tttgaactgg gggagaacga caaaaaccag
     1321 atgctgtaca gcatgaaggt atgcccaccg ctgtggcgca
//
LOCUS       DAZOZD010000490         1356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOZD010000490 DAZOZD010000000
VERSION     DAZOZD010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.498"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..654
                     /gene="erm"
                     /locus_tag="ACHUPB_004697"
     CDS             <1..654
                     /gene="erm"
                     /locus_tag="ACHUPB_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFR0761262.1"
                     /translation="ETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLK
                     LNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIVEEGF
                     YKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPDKYWK
                     LYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          483 a          243 c          226 g          404 t
ORIGIN      
        1 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
       61 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      121 gaaaaattaa aactgaatac tcgtgtcact ttaattcacc aagatattct acagtttcaa
      181 ttccctaaca aacagaggta taaaattgtt gggaatattc cttaccattt aagcacacaa
      241 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      301 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      361 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaagagta
      421 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      481 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      541 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      601 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
      661 gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa gggaatgtag ataaattatt
      721 aggtatacta ctgacagctt ccaaggagct aaagaggtcc ctagactagc aagaagtaca
      781 caagaagcct taaagattat agaaaagctt ttccatgaaa tgcttaacat tatttaatgt
      841 taacatgtgg ctttgtggta actaccacga gtggtaactt gtaacatgaa aaaagttaca
      901 agttacaagt ggtttattta cgaacataaa ggctaggaaa gcacttgata agtatatcaa
      961 gtgctttttt tccgttcgat tttactatcc aagataccgc ttgacgtacc taatatttta
     1021 gtgtgctaag ggctttgctt tttctggtta tagttcgttc aaaaaagacc gcaagggtaa
     1081 atgcacgatc atagatcgcc cttgcagctt tttaatctct ccactttttt ataaccaagc
     1141 aaaccaccaa gcacactgaa aaaataatta ggaactaaag cgaagtgcgt atcttggata
     1201 aatcttcact cgattttaaa agattaagca cccagccaaa gcgcctaaaa aaataatttt
     1261 tttaaagaaa ggaagtaatt taaatgacta caattttaag cgactacaat aaatctatcc
     1321 taattgaatt actaaacacc aatcgccaaa ttatcg
//
LOCUS       DAZOZD010000491         1355 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOZD010000491 DAZOZD010000000
VERSION     DAZOZD010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.499"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            89..>1355
                     /gene="istA"
                     /locus_tag="ACHUPB_004698"
     CDS             89..>1355
                     /gene="istA"
                     /locus_tag="ACHUPB_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0761263.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          345 a          377 c          345 g          288 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttg
//
LOCUS       DAZOZD010000492         1346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOZD010000492 DAZOZD010000000
VERSION     DAZOZD010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.500"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..184)
                     /locus_tag="ACHUPB_004699"
     CDS             complement(<1..184)
                     /locus_tag="ACHUPB_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0761264.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
     gene            complement(229..1215)
                     /locus_tag="ACHUPB_004700"
     CDS             complement(229..1215)
                     /locus_tag="ACHUPB_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0761265.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          371 a          298 c          272 g          405 t
ORIGIN      
        1 cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt aatgtacttg
       61 gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc agagcaagca
      121 tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg cggttaaata
      181 tcattgtaag catcttggaa aatcagggcc agagactggc ccggaatatt attgagcagc
      241 gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt cgtcaaacag
      301 aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac cccccatagc
      361 tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt gataagcttc
      421 ggatttcttc gctgctgttt cgaggatttg tttctgttct ggggttaatt tattccatgt
      481 tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag aaaaaacttt
      541 ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg cgccatctac
      601 tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg gtgaaccacc
      661 catcagttca atcattttga tggtggtcgg acttggctga acacgaattt tcagcccttt
      721 aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac ccgctacata
      781 agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt taccaacttc
      841 accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca aattatagat
      901 agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca tatccagtgc
      961 gccattttgc aacaattcca tagtttctcg cgcattaccc atctggctgc tgggataaat
     1021 acggatcacc atattacctt ctgacagctg ttttacctct ttcgccagct cttcaaaact
     1081 ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca ctttttctgc
     1141 ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg agcgggtaaa
     1201 tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg atatctaatt
     1261 gtgcttatca gtgctttgtg cgacaaattg gttaacctat ctgacaactt gtctatgttt
     1321 atcacaactt gaaataaagt cagaaa
//
LOCUS       DAZOZD010000493         1346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOZD010000493 DAZOZD010000000
VERSION     DAZOZD010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.501"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            23..1195
                     /locus_tag="ACHUPB_004701"
     CDS             23..1195
                     /locus_tag="ACHUPB_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0761266.1"
                     /translation="MKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEY
                     NCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLW
                     PVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCG
                     CLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQWRSLVL
                     KAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKTG
                     ETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPV
                     AKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          318 a          330 c          437 g          261 t
ORIGIN      
        1 gtccgctttt actccccgcc cgctgaaacg tctgttcacg gccaaccagt gctggacgtc
       61 cttcctggat gcgggcggtc tgcgcgatat cgaggttgag gctgtcacaa aaatgctggc
      121 ctgcggcacg cggatactgg gagtgaagga atacaactgc gataaacctg agtgccccca
      181 cgtcagatac gtcactaact catgcggcag ccgtgcctgc ccgtcctgcg gaaaaaaggc
      241 cacagacctg tggatagcga cacagctgaa tcgtcttcct gactgcgact gggtacatct
      301 ggtcttcacc ctgccggaca cgctgtggcc ggtgttcgaa agcaaccggt ggctgctgaa
      361 tgacgtgtgc cgtctggcgg tggagaatct gctgtatgcc gcccggaaac gggggcagga
      421 acccggtatc ttctgcgcca tccacacgta tggccgtcgt ctcaactggc atccgcatgt
      481 acatgtgtct gtaacctgtg gatgtctgaa taagcatggt cagtggaaaa agctgagctt
      541 cctgaaagac gcgatgcgtt cgcggtggat gtggaatatg cggcaacggc ttctgaaagc
      601 gtggtcagag gggctggcga tgccggagtc gttgtcacat atcacgacgg aatcacagtg
      661 gagaagcctg gtgctgaaag ccggaggaaa atactggcat gtgtacatgt cgaaaaaaac
      721 ggccggcggg aggaatacgg cgcgctacct gggtcgttat ctgaagaagc cgccgatagc
      781 ggcctcccgg ctggcacatt acaacggagg ggcgagcctg agcttccgtt acctggacca
      841 caaaacggga gaaacggcga cggaaacgct gacacagcgt gagctggtcg cgaggctgaa
      901 acagcacatc ccggagaagt tttttaagat ggtgaggtac ttcgggttcc ttgccaaccg
      961 ggtgtgtgga gagaagctgc cgcaggtgta ccgtgctctg gggatggata aaccggaacc
     1021 agtggcgaaa gtgtgctatg cacaaatggt gaaacagttc ctgagtcgtg acccgttcga
     1081 atgcgtgctg tgtggcggcc ggatggtata ccgccgggcc attgcggggc tgaatgtgga
     1141 ggggctgaag aaaaacgcgc gggatatcag tctgctgagg tatatgccgg cctgacacag
     1201 gctaagtgtg cccgggaatc ggtgcaagta taaaaaacag cctgttgcgg tataagaatc
     1261 gaccatgcag agagagaaaa caccggagtg gtggggaaaa acagaaaggc tcacgtggca
     1321 taaggcgcgg acaggggtat cgctta
//
LOCUS       DAZOZD010000494         1342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOZD010000494 DAZOZD010000000
VERSION     DAZOZD010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.502"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHUPB_004702"
     CDS             <1..185
                     /locus_tag="ACHUPB_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761267.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            195..>1342
                     /gene="yicI"
                     /locus_tag="ACHUPB_004703"
     CDS             195..>1342
                     /gene="yicI"
                     /locus_tag="ACHUPB_004703"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0761268.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          319 c          351 g          337 t
ORIGIN      
        1 ccacgattag catcattatt gcgctattca cgattgttcc ggcgatctgt tatttgctga
       61 gcgcgattat cgctaaacgc tactactcac tcacgacgca caatctgaaa accgttatgg
      121 aacagctggc tcagggtaaa cgccgttgcc agcaacaatt cacctctcaa gaagtgcaga
      181 actaaggaac ggcaatgaaa attagcgatg gaaactggtt gattcaacct ggcctcaatt
      241 tgattcaccc gcttcaggtg ttcgaggttg aacagcagga taatgaaatg gtggtctatg
      301 ctgccccccg tgatgtgcgt gaacgtacct ggcagcttga tacgccttta tttacgctgc
      361 gctttttctc cccacaggaa ggtattgtcg gtgtgcggat tgagcatttt cagggggcgc
      421 tgaataacgg tcctcattat ccgctcaata ttttgcagga cgtgaaggtc acaatcgaaa
      481 acacagaacg ttatgctgag tttaaaagtg gcaacttaag cgcgcgtgtc agcaaaggtg
      541 agttctggtc actggatttt ctgcgcaacg gcgaacgtat taccggtagt caggtgaaaa
      601 ataatggcta cgtgcaggac acgaataatc aacgcaatta tatgtttgag cggcttgatc
      661 ttggcgttgg cgaaacagtt tacggtctgg gagagcgctt tactgccctg gtgcgcaatg
      721 gccagacggt agagacctgg aaccgggacg gcggcacaag tactgaacag gcgtataaaa
      781 atatcccgtt ctacatgact aaccgtggtt atggggtact ggtcaatcat ccccagtgtg
      841 tctcttttga agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt gagagtgaat
      901 atctcgaata ctttgttatc gacggcccga cgccgaaagc ggtacttgat cgttataccc
      961 gctttactgg tcgtccggcg ctgccgcccg cgtggtcctt cggcctgtgg ctaaccactt
     1021 catttaccac caactacgac gaagcgacgg taaacagctt tatcgatggt atggcggaac
     1081 gcaatctgcc gctgcatgtt ttccactttg actgtttctg gatgaaagcc ttccagtggt
     1141 gcgattttga gtgggacccg ctgactttcc ctgacccgga agggatgatc cgccgcctga
     1201 aagcgaaagg gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa aaatcccccg
     1261 tctttaaaga gttacaagag aaaggctatt tactcaaacg cccggacggt tcgctatggc
     1321 agtgggataa atggcagcca gg
//
LOCUS       DAZOZD010000495         1325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOZD010000495 DAZOZD010000000
VERSION     DAZOZD010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.503"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1116
                     /locus_tag="ACHUPB_004704"
     CDS             <1..1116
                     /locus_tag="ACHUPB_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0761269.1"
                     /translation="TLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYI
                     FRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYS
                     LYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCC
                     NLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLTNIYN
                     RRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIR
                     PDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGT
                     VFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT          356 a          282 c          268 g          419 t
ORIGIN      
        1 acactcattt taagccttgc atttttaagt ggccttatct atttagttga aaccattgta
       61 attatccata aaccaattaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct
      121 attttctata ttttccgcca actcagtttt atttgtttaa cctcgctggc actcttttgt
      181 tatggaaaag acaacatcct tgacaacaat aagaaaaaaa cgggaatcct gttgctggcg
      241 ctgattcctt ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac
      301 tattctttgt atgtcgtcga ctactgtccg gacaaccata ctgcgacctg gggaatcaac
      361 tatacaaaaa tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca
      421 cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc
      481 aacttacttc tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg
      541 attgaagttt ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcagaaa
      601 ctgcaactct ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc
      661 tactttttca acagcgtaga gtcattattg tcgagacctg ttgttaagga cttctgtgtc
      721 atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt
      781 gataaagtgc tggtttcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt
      841 ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag
      901 gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac
      961 agatatgatg ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac
     1021 acgcgtaata tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc
     1081 gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc
     1141 tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc
     1201 ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt
     1261 tttctcgttt cactgaaccg aaggagtgcc atttatcatg aaattgcacc atagaatgct
     1321 ccggc
//
LOCUS       DAZOZD010000496         1298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOZD010000496 DAZOZD010000000
VERSION     DAZOZD010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.504"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            94..1068
                     /gene="cysB"
                     /locus_tag="ACHUPB_004705"
     CDS             94..1068
                     /gene="cysB"
                     /locus_tag="ACHUPB_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0761270.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          342 a          300 c          292 g          364 t
ORIGIN      
        1 gttagcacct tttttattat taaatcgtat tagtcacccg ccaggtgtga cgaaaaaacg
       61 atgttctgat ggcgtctaag tggatggttt aacatgaaat tacaacaact tcgctatatt
      121 gttgaggtgg tcaatcataa cctgaatgtc tcatcaacag cggaaggact ttacacatca
      181 caacccggga tcagtaaaca agtcagaatg ctggaagacg agctaggcat tcaaattttt
      241 tcccgaagcg gcaagcacct gacgcaggta acgccagcag ggcaagaaat aattcgtatc
      301 gctcgcgaag tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggaga gcacacctgg
      361 ccggataaag gctcactgta tatcgccacc acgcataccc aggcacgcta cgcgttacca
      421 aacgttatca aaggctttat tgagcgttat ccccgcgttt ctttgcatat gcaccagggc
      481 tcgccgacac aaattgctga tgccgtctct aaaggcaatg ctgatttcac tatcgccaca
      541 gaagcgctgc atctgtatga agatttagtg atgttaccgt gctaccactg gaatcgggct
      601 attgtagtca ctccggatca cccgctggca ggcaaaaaag ccattaccat tgaagaactg
      661 gcgcaatatc cgttggtgac atataccttc ggctttaccg gacgttcaga actggatact
      721 gcctttaacc gcgcagggtt aacgccgcgt atcgtcttca cggcaacgga tgctgacgtc
      781 attaaaactt acgtccggtt agggctgggg gtaggggtca ttgccagcat ggcggtggat
      841 ccggtcgccg atcccgacct tgtgcgtgtt gatgctcacg atatcttcag ccacagtaca
      901 accaaaattg gttttcgccg tagtactttc ttgcgcagtt atatgtatga tttcattcag
      961 cgttttgcac cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt gcgctctaat
     1021 gaagaaattg aggtcatgtt taaagatata aaactgccgg aaaaataatt tcttgcgtta
     1081 ttttcggcac cttttatgta gcgaaggtgc cggaatatat tctcttttgt tacttatttc
     1141 tataccgtgc acaatatttt tatccctcat atttataggg taaattacct gtaaagtagt
     1201 gtctaattac ccgcctcgca aactccagtt ttcaaatatc tatttcaggt caaaagattg
     1261 aattattcac tggagacgat tcgtaaattc gctggatt
//
LOCUS       DAZOZD010000497         1296 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOZD010000497 DAZOZD010000000
VERSION     DAZOZD010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.505"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..654)
                     /locus_tag="ACHUPB_004706"
     CDS             complement(<1..654)
                     /locus_tag="ACHUPB_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0761271.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     "
     gene            complement(680..>1296)
                     /gene="rfaY"
                     /locus_tag="ACHUPB_004707"
     CDS             complement(680..>1296)
                     /gene="rfaY"
                     /locus_tag="ACHUPB_004707"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0761272.1"
                     /translation="EILAYNFKTVKVLRNINDTKVSLIDTKYCRYVFKVFAPKTKRNE
                     RFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGV
                     ELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAE
                     RKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          400 a          269 c          226 g          401 t
ORIGIN      
        1 aatatcctga tcaaggcatt tatacttcgt gtgctgcggg tcacttgcca gcatagcaat
       61 ggctttattc ataaaatcat ttttcgccca tgcttcaagc tgcagatata ctacaccggc
      121 gttgaaataa ttccctggaa gtccctctat agccagacgc ttcgctggtt tttcctgcat
      181 aaaaggcaca tctttaataa cagctgcaaa cttatcccca tcaaaattaa tatcaagcaa
      241 tccagctaat gagcctttac agatgacatc agcatcgata tataacagat tttcaatagt
      301 ttcactcaga tactggaacg caactaaacg gaagaatgtt gcataagacc actgtttcag
      361 gctggggaag atatcaataa aggcactgga gactttataa attcttactt caacgttagc
      421 atttttgctg gtaaaagcat taacacgttg tagataatct tcatcaatgt agtcggtaaa
      481 gagatgaaac ttaaccgcaa ggtccggatt atgcataaca actgattgca tagatactgc
      541 cgcaccaaat aaaaagttac ggtcaatacc gtaagcgata tttaacacac gatcggtgtt
      601 agcaatttcc ccagagacag cgacttcagt aatactctca gctaataaat ccatgttggt
      661 tccgttttga ctgtgtggtt tacgctttgc cttttaattt tttaataaat ttgcgtagtt
      721 tggttctgta gattactgaa tagtagccat aatcttttat ttcgttggca atacccaggt
      781 gacgttccat ggccagacga tcacgagctt tacgctctgc cgtacagctt ttaccggata
      841 ggtcaattat cctgacacca tctttactaa cgataaagtt accccggtgt gggtcgcccg
      901 atagcatatt taaggcatgc aatttttcca ttgaagcctt aatttctgct ttaacatctt
      961 ctggaatgat cggcatatca ttgagctcta cgccctcgac atactcgata agcatgataa
     1021 aaacactggc atagttgaaa attttacgct cagccagaaa ataaaaatca ttaggaaaag
     1081 taagacctgc acttctgaca cgatcggttt caacgatcaa attctgatag tagtcacctt
     1141 ttacaaaaga ttttaaaaaa cgttcattcc ttttcgtttt cggggcaaag actttaaaaa
     1201 catagcgaca atatttcgta tcaatcaatg atactttggt atcattaata ttacgtaata
     1261 cttttactgt tttaaaatta taggccagta tttcat
//
LOCUS       DAZOZD010000498         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZOZD010000498 DAZOZD010000000
VERSION     DAZOZD010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.506"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..1112
                     /locus_tag="ACHUPB_004708"
                     /pseudo
     CDS             1..1112
                     /locus_tag="ACHUPB_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1090..>1294
                     /locus_tag="ACHUPB_004709"
     CDS             1090..>1294
                     /locus_tag="ACHUPB_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761273.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAZOZD010000499         1294 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOZD010000499 DAZOZD010000000
VERSION     DAZOZD010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.507"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHUPB_004710"
     CDS             <1..154
                     /locus_tag="ACHUPB_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR0761274.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="ACHUPB_004711"
     CDS             258..1172
                     /locus_tag="ACHUPB_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761275.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          378 a          267 c          293 g          356 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cgatggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tatt
//
LOCUS       DAZOZD010000500         1285 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZOZD010000500 DAZOZD010000000
VERSION     DAZOZD010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.508"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..595)
                     /locus_tag="ACHUPB_004712"
     CDS             complement(<1..595)
                     /locus_tag="ACHUPB_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761276.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1025..1285)
                     /locus_tag="ACHUPB_004713"
     CDS             complement(1025..1285)
                     /locus_tag="ACHUPB_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761277.1"
                     /translation="MDAGIAINFDKLLKLMADNVENISDFIQSVEFNEPRMMLPITDS
                     NMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTDSVRVH"
BASE COUNT          392 a          273 c          211 g          409 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag aaaaataaga gagaattgct ctcccataat taaaattatg
      901 ccatgcttaa ggttatttgt caacagcttt ttaacaggtt gataacatca attcttattt
      961 accgcattgt taataataca accaacaaat aagccaagca acgtccaagc aattattaaa
     1021 cccattagtg aaccctcaca gaatccgtca ccagaatatt atcactgtac aggattgtca
     1081 cagcaccttt caaaaattgc tctggggaaa tactgtatat gttcgtttgc tcgataacca
     1141 atcttttcat gttgctgtct gtaattggta gcatcattcg aggctcgtta aactccaccg
     1201 actgaataaa gtcagagatg ttttctacat tatcagccat cagctttagt aatttatcaa
     1261 aattaattgc gatacctgca tcaat
//
LOCUS       DAZOZD010000501         1283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOZD010000501 DAZOZD010000000
VERSION     DAZOZD010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.509"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            133..1152
                     /locus_tag="ACHUPB_004714"
     CDS             133..1152
                     /locus_tag="ACHUPB_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0761278.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          286 c          357 g          272 t
ORIGIN      
        1 ccacacaatt aaacacggag cagtatgatg gttaaaaggg tgaaactggc aggtgtggag
       61 cctcgcagat ttccggcatc atagtgccca atacgggatg cataaagctc cctcagactg
      121 gagactccac aaatgaaata tacaccggtt ggcgttgata tcgcaaaaca tgtcattcag
      181 attcacttca tcaatgagca cacaggtgaa gtggttgata aacagttgcg tagacaggat
      241 tttctgacgt tcttcggcaa ccgtgagcca tgcctgattg gtatggaggc ctgtggaggt
      301 tctcagcact gggcacggga actgacaaaa cttggtcata aagtccggtt gttgcaggcc
      361 cgcttcgtta aggcattcgt catgggcaat aagaatgatg tgatggatgc ccgggctatc
      421 tggatggcgg ttcagcagcc gggtaaagaa atcgccgtaa aaacagaaga acagcagtcg
      481 gtactggttc tgcaccgtac ccgcatgcaa ctggtgaagt tccggaccgc acaaattaat
      541 gccctgcacg ggacgttact ggagtttggt gaaaccatcc acaaaggccg ggcagcgatg
      601 gagcgggagt tccccgaagc actggaacgg atgaaagaga gactgccacc gtatctcatt
      661 atggttctgg aaaaccagta caaccgactg aatgagctgg actcactgat agaggatatt
      721 gaaaaacagc ttaccagcgt ggcgaggcag aatgaaacct gtaagcggtt gctggatatt
      781 cctggcgttg gaccacttat tgcgacggca gcggtggcca ccatggggga agcatcagcg
      841 tttaaatcgg ggcgagagtt cgccgcatat gttggtctgg ttccaaaaca aactggctcc
      901 ggagggaaag tacgtctgct ggggataagc aaacgtggtg acacttatct caggacatta
      961 tttatccacg gtgcaagagc ggtggcatta gtagctaaag agcctggccc gtggataacc
     1021 gaactgaaaa aacgtcgtcc agccagtgtg gcaatcgtcg ccatggcaaa caagctggca
     1081 cgaacagtat gggcgataac cgcccatgac cgtaagtatg acaggaacca cgtcagtatc
     1141 agaccatatt aatcgctgat accattaaac aatgaactct taacaaaagg gtgaatgctg
     1201 aaaggttgct atggcggcca gagtgatgac aaagacaggt aagaccgtga ctcactaaac
     1261 ctgaacagta ttttgggctt gaa
//
LOCUS       DAZOZD010000502         1279 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOZD010000502 DAZOZD010000000
VERSION     DAZOZD010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.510"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPB_004715"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="ACHUPB_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR0761279.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTRVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          231 c          287 g          401 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggtta ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagcgctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccagctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tagcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAZOZD010000503         1266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOZD010000503 DAZOZD010000000
VERSION     DAZOZD010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.511"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHUPB_004716"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHUPB_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0761280.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHUPB_004717"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHUPB_004717"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0761281.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          349 a          286 c          268 g          363 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaaactga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttgcacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt attccttata
     1201 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1261 ttttgc
//
LOCUS       DAZOZD010000504         1260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOZD010000504 DAZOZD010000000
VERSION     DAZOZD010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.512"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(19..>1260)
                     /locus_tag="ACHUPB_004718"
     CDS             complement(19..>1260)
                     /locus_tag="ACHUPB_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0761282.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          332 a          297 c          227 g          404 t
ORIGIN      
        1 gaaccctaac aaaaaatact aagcgtccac ctcttccata tcaccagcag gataaatcct
       61 tgttactggt tcaccaaaaa taagcatatc atctaatgac tgcatctcat cggttgcttc
      121 ttcaagcgtt aaggtgtggt catctttacc atcaagagga aaaccacgaa tcatatttcc
      181 attgtgaaga tatatatcca ccagcttttc attaccagaa acaacaatct ctttcaccag
      241 acgcttaata ataatctggg cttcagttct cccctctaca gactccatat caagaccgct
      301 taaatccaag cttgatacag attttaccct cgccttttct cgtgataact gaactatcag
      361 ttcatctttt tcttcctgtt taacccgtag tgcttcttga atctgagtga tgttaggaag
      421 ctgtatcagc gtctcagtga gtttttccag ttcaccatca acggtgttta atcttctttg
      481 tagttcatct aatttagctg tatcggtagc ttcatcgtgt agctgtgaca gtttgctaaa
      541 cagacgacta caaagctgtg tatcaagtaa ctttctggac actgtaccgt agctacaacg
      601 ggactcctta ttaccattgc aacggtaagt accttgataa acgggtttac gtatccctgt
      661 aggagtcatt acaagcccac acttacaacg gataagcccc ttcagtatgt tcacatatag
      721 ccagtgttca ccgctggatg ttttcccctt ccctgtttcc tctaacagtt gctgtacagc
      781 gtaaaactta gagtctgtga caatctgagg atagaaccct tcaatttctt cacgtaacac
      841 tgttttacgg tcttctgtag aaatcttagc gggaaggtaa cctaatacac ttcgtgaagt
      901 caggatgtgt ttaacgctta atgctgacca gttgccatcc ggtttcttag tcttggatat
      961 tctgacctta cgctgattaa tggtcgggta accttgttcg tttagccatt tacagatagc
     1021 aaacatcgac ataccagaaa gacgcttgtc atagatgata ttcaccagtg acgctttatc
     1081 ttcatcaacg atgtagtatg aaccatcttc tgatagctct aaccatccgg gggcttgctt
     1141 acccataggc ttcttaagtt ctcttgcaag ctgtgtttta tggttccaga cctgagctat
     1201 acgcttttgc ttaacaagag attcttggtg tgctctctca gctatgatga tgtgcttaat
//
LOCUS       DAZOZD010000505         1255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOZD010000505 DAZOZD010000000
VERSION     DAZOZD010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.513"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(274..576)
                     /locus_tag="ACHUPB_004719"
     CDS             complement(274..576)
                     /locus_tag="ACHUPB_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761283.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(573..1199)
                     /locus_tag="ACHUPB_004720"
     CDS             complement(573..1199)
                     /locus_tag="ACHUPB_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFR0761284.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
BASE COUNT          341 a          280 c          232 g          402 t
ORIGIN      
        1 tttgttttcc tcagaggatg aaggagatac cgaatgtctg accctgtacg gggttcagtg
       61 agttagctgg cctggatgta accactcatt caactcaatt ctcttagcgt ggaaaaattt
      121 cccttttcca agcggacccc tattaatatt tgttttgttt taggttctat tcaacatagg
      181 tcatcctaaa atacattgaa gataacaaga taacaagata acaagatagc aagataacaa
      241 gataacaagg tgttatcttg ttatctcaag gtgttatctt tgttttactt ctgctatcaa
      301 tcgtgatacc tctgtttttg atagtgtttt aagtgcttct accgccgccc tgacaacatc
      361 agaacgattc actctgcctt tacctgaaac catctcagcc gttataaggg catcaatgtc
      421 ttttaggttt tcttcaaaaa aagaaatact cactggtttt gctttgctta caaccgatga
      481 tttatctgat gactttttgg tattatcaag gccagccgtg ccaccgttaa taaatgctgc
      541 ctcgtcacga ggtgaagtgt ttgctctatt tctcacagaa taacctcttt cactaacgct
      601 tcgatttctt gctgggcttt atcattttgc gtttcaaaaa ttgtcattga ttcggcccaa
      661 gcatctcgat gtgctttacg atcacatacc cgggagtttg ccagatgcat ttcagggtaa
      721 tccttaagta cttcagctgc ctcattggct tcattaacaa acatatttgt gggcgtcatg
      781 tttaaaacga gatatccccg aacatcctca ttaaaatctt ttgctgctgt aaaaacggag
      841 catgtatgag gcacaacatc caggtccatc tgagatgggc gcaaaggaga aatgaataca
      901 tctgcggcca tcaatccact acgcatttct gcgctatcac ggccagcaca gtctgcaata
      961 acaaaatcat aggatttttc gtgttccttt aacatggctt tgatgttacc tgtcgcgcct
     1021 gtaaccggaa tatgagggag atcttcaggg cgattgttgt accaggttaa tactgatttc
     1081 tggtcatctg cgtcgacaat aatcactcta ttgcctagtg acatcagata cgcagctata
     1141 gataccgcca gtgtactttt acctacccca cccttttgac tacctaatac tattatcatt
     1201 atagcctcat attaagctgt taagttaaca acttaatatt gccatctgaa cgcga
//
LOCUS       DAZOZD010000506         1247 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOZD010000506 DAZOZD010000000
VERSION     DAZOZD010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.514"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..512
                     /locus_tag="ACHUPB_004721"
                     /pseudo
     CDS             1..512
                     /locus_tag="ACHUPB_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            677..>1247
                     /locus_tag="ACHUPB_004722"
     CDS             677..>1247
                     /locus_tag="ACHUPB_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0761285.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLICLCY
                     SQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSS
                     VSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLV
                     TEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          338 a          278 c          301 g          330 t
ORIGIN      
        1 aacgacctga ccagctgtgg gtggctgatt ttacttacgt cagcacatgg cagggcttcg
       61 tctatgtggc gttcatcatt gatgtgtttg ccggatacat cgtgtggtga cgagtctcat
      121 cgtctatgga aacgacattc gtgctggatg cactggagca ggcgttgtgg gcccgtcgcc
      181 cgtctggcac catccatcac agcgataaag gctctcagta tgtgtcactg gcctataagg
      241 cgcgactaaa agaagcaaaa ctgctggcat caacagggag tactggtgac tcgtatgaca
      301 acgcgatggc agagatcatc aagggccttt acaaagcgga ggtaatacac cgtaagagct
      361 ggaaaaaccg gacagaagtg gagctggcca cactcacgtg ggtggactgg tataacaatc
      421 gacgattgct ggaaaggctg ggccatatcc caccggcaga agcagaaaaa gcttattatg
      481 cttccatcgg aaatgatgat ctggcagcct gagttcacag ataaaacact ctccaggaaa
      541 cccggggcgg ttcagttcac aaaaacacat taatgcagta actatatttt cctttctggt
      601 gataacgtcg ggttatcatt agcttcttca gctattttac ttttatatcc cttgtaaaca
      661 tcatggagaa tccatagtga ataaagttta ttctcttaaa tattgccccg tcaccggggg
      721 gcttattgct gtctctgaac ttgcccgcag ggtaataaaa aagacatgcc gaagattaac
      781 gcatattctt ctaatctgtc tgtgttactc tcagatatcc ctggcgggta ttgtccgttc
      841 cgatattgcc tatcagattt atcgtgattt cgccgaaaac aaagggcttt ttgtacctgg
      901 tgccaatgat attccggtat atgataagga cggaaaactt gtgggaagac tgggtaaagc
      961 cccaatggcc gatttcagca gtgtgagctc aaatggcgtt gctacgcttg tatcgcctca
     1021 gtatatcgtc agcgtaaagc ataacggagg atatcggagt gtgagctttg gtaatgggaa
     1081 aaatacatat tcccttgttg accgtaataa ccacccttct attgacttcc atgctccacg
     1141 tctgaataaa ctggttacag aagttattcc ctcagcggta acatcagaag gaaccaaagc
     1201 caatgcttat aaatacactg aacgttacac cgctttttat cgggtgg
//
LOCUS       DAZOZD010000507         1239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOZD010000507 DAZOZD010000000
VERSION     DAZOZD010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.515"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1087
                     /locus_tag="ACHUPB_004723"
     CDS             join(<1..183,183..1087)
                     /locus_tag="ACHUPB_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
                     /protein_id="HFR0761286.1"
                     /translation="ERIGVAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENA
                     RLKRQLAEQAEELAILPKGRDILCEAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWY
                     TWCQRRTGISPRQQFRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAA
                     SLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGW
                     LYLAVVIDLWSRAVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDRGSQYCSA
                     DYQALLKWHNLRGSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENQNLA"
BASE COUNT          284 a          316 c          360 g          279 t
ORIGIN      
        1 ctgaacgcat cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct
       61 atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg
      121 ccgctgaaaa tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
      181 tccaaaaggc cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      241 acatcaggct gagttcagca tcaaggcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      301 ctggtatacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca
      361 ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc
      421 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
      481 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta
      541 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
      601 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
      661 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
      721 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
      781 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
      841 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
      901 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
      961 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     1021 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     1081 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     1141 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     1201 gcaaagttat ccacaggcac tgtgcattta aaaaactta
//
LOCUS       DAZOZD010000508         1227 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZOZD010000508 DAZOZD010000000
VERSION     DAZOZD010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.516"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            67..1149
                     /locus_tag="ACHUPB_004724"
     CDS             67..1149
                     /locus_tag="ACHUPB_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0761287.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          349 a          258 c          274 g          346 t
ORIGIN      
        1 gattagatga atatttatcg cgcaatgaca tcatttttta ataatagttc aaaaaaaggg
       61 ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatcagt actgatggcg
      121 atgtctgctc aggcagctga aatttataat aaagacagta acaagctgga tctgtacggg
      181 aaagttaatg ctaagcacta cttctcctct aatgatgcag atgatggtga tactacttat
      241 gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc aactgactgg tttcggtcag
      301 tgggaatatg aattcaaagg caaccgcgct gaatctcaag gttcctccaa agataaaacc
      361 cgtcttgcct tcgctggcct gaaattcggt gactacggct ccatcgatta cggccgtaac
      421 tacggtgtag catacgacat cggtgcgtgg actgacgtcc tgccagaatt cggtggtgac
      481 acttggactc aaacagatgt gttcatgact ggtcgcacca ctggtgttgc aacttatcgt
      541 aacaacgact tctttggtct ggttgatggt ctgaactttg ctgctcagta tcagggtaaa
      601 aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt tcggtttctc cactacttat
      661 gagtatgaag gattcggtgt aggtgcaacc tatgctaaat ctgaccgcac taataatcag
      721 gttatctacg gtaacaacag cctgaatgca tctggtcaaa atgctgaagt atgggcagct
      781 ggtctgaaat atgatgcgaa caacatctat ctggctacca cctattctga aacccagaac
      841 atgactgttt ttggtaataa ccatattgcc aacaaagcac aaaacttcga agtagttgca
      901 caatatcagt tcgacttcgg tctgcgtccg tccgttgctt acctgcaatc taaaggaaaa
      961 gacttgggtg cgtggggtga tcaggacctg gttgaatata ttgatgtagg tgcaacctat
     1021 tacttcaaca aaaatatgtc cacttttgtt gattacaaaa tcaacctgat tgataagagc
     1081 gatttcacga aagcatctgg cgttgctacc gatgatatcg ttgctgtagg tatggtttac
     1141 cagttctaat ttgattacta aaagatatgt tgcgggaggc tttgcctccc caacatataa
     1201 gtggctccct caagccactt cctttag
//
LOCUS       DAZOZD010000509         1224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZOZD010000509 DAZOZD010000000
VERSION     DAZOZD010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.517"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>1224)
                     /locus_tag="ACHUPB_004725"
     CDS             complement(<1..>1224)
                     /locus_tag="ACHUPB_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR0761288.1"
                     /translation="WYMNGHIGELDKLVPHLITEHKIYGRDRITPRTVSAEDGVIKKQ
                     NNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYI
                     GIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGYPKE
                     TSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQ
                     YKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIG
                     DEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHP
                     ILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVEN
                     MFQENNNGFTALFYG"
BASE COUNT          361 a          254 c          228 g          381 t
ORIGIN      
        1 ggccataaaa taatgcagta aaaccattgt tattttcttg aaacatattt tcgacaaaag
       61 ccggtatggt tcttgctgta ttcggtacgc agcagacagc agcctcctca tgagtagggg
      121 agtatttata tctggtatta aactgattac tgtcaggatg tttcctttca tttgcttcat
      181 aacaattatc agtcttacag tacattatcg cgtgaagtat gggatgctgc atgccaaaac
      241 tagcattata gaatagccac tcagcatcat cggccagacc aatattagtg gtcagtttta
      301 ttaacataac ataagtgcta aatagttcat gaacagaaca atattcatag cctgtataag
      361 tggcattagc tgtgcgtcct gtaatccact catcaccaat gggagcgcca gatggtgtaa
      421 gataatattt caactgggat aaaaaaatat ctaatgcagt ttgatactgc ggccgttggc
      481 tcgctgcgat ggctaatgcc ctaatatgct catacgtatg tacgccatga cctttaagaa
      541 ggtaaccagg gtctatgaca tttttatatt gcagatcttg ctccgacacg catcctgcgg
      601 aataatcttc atacagccac aagcaatatg agagatattc aatgttattg gttatttcat
      661 aaaggcggta gaaaacatct gttattgtta atccgtggct atgtccagca aaaccctgtt
      721 ccacaacgaa aggatgtgac gtttctttag ggtagccagc cataatgcat tgtgcggctt
      781 gggtaagaga agttaataaa ctctcattac gggtggactc ataacatcca agcacaaaac
      841 gcaaggcagt agcttgtgcc cagaactctc cgttttctgc tttacagtta aattttaatg
      901 atgaatcata aattccaatg tagccatcat catcttgcgt atcgataagt tccaaacata
      961 aatcgctcgc tttttcctgc cattctttat tatctaacaa gtaggcactg cggcaatatc
     1021 catccttcca attcgattga gtttcactat cccaccaata atattgcatc atgttatcat
     1081 cgggattatt ctgtttttta ataacgccat cttcagcaga tacagtgcgt ggagttatac
     1141 gatctcttcc atatatttta tgctccgtaa taagatgggg aactaattta tccagttctc
     1201 ctatatgacc gttcatatac catt
//
LOCUS       DAZOZD010000510         1215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZOZD010000510 DAZOZD010000000
VERSION     DAZOZD010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.518"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            3..1198
                     /gene="bluF"
                     /locus_tag="ACHUPB_004726"
                     /pseudo
     CDS             3..1198
                     /gene="bluF"
                     /locus_tag="ACHUPB_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          345 a          259 c          268 g          343 t
ORIGIN      
        1 taatgcttac cacccttatt tatcgtagcc atatacgtga cgacgaacct gtcaaaaaaa
       61 tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca gtctgacgta acagggatct
      121 tactgtttaa tggttctcat tttttccagc ttctggaagg tccggaagaa caggttaaaa
      181 tgatatatcg ggctatatgc caggatccac ggcactataa tattgttgag ctgctgtgcg
      241 attacgcgcc tgctcgccgt tttggcaaag cgggaatgga attatttgat ttgcgcctgc
      301 acgagcgaga tgacgtttta caggccgtat tcgacaaagg cacatcaaaa tttcagctaa
      361 cttatgatga cagagcgcta caattttttc gtacttttgt ccttgcaacc gaacaatcaa
      421 cctatttcga gatccctgcc gaagactcct ggctttttat cgctgacgga tctgataaag
      481 aacttgattc ctgtaccctt tcaccaacta taaacgacca ctttgccttt catcccttat
      541 cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagcccat cagccatagc
      601 ggttgggcag cgtaaagacg gggaaatcta cacagcggat ctcaaaagta aggcgcttgc
      661 attcgcgatg gcacacgcac ttgagctcgg tgataaaatg atttcaatca atctattacc
      721 tatgaccctg gttaacgaac ctgacgcagt ctctttttta cttgatgaaa taaaggccaa
      781 tgctctggtg cctgaacaaa tcatcgttga atttactgaa agtgaagtta tatctcggtt
      841 tgatgagttt gcagaagcga ttaaatcgct taaggctgtc ggtatcagtg tagcaattga
      901 tcattttggc gcaggttttg ccggtttgtt actcctgtca cgcttccagc ctgacagaat
      961 taaaatcagt caggaattga ttaccaatgt tcataaaagc gggccacggc aggcaattat
     1021 tcaagcgatc ataaaatgct gtacatcact tgaaattcaa gtcagtgcta tgggcgtggc
     1081 aacaccagaa gagtggatgt ggcttgaatc cgcaggaatt gagatgtttc agggagatct
     1141 gtttgctaaa gctaaattga atggtatccc ttccatagcg tggccagaga aaaaataatt
     1201 ttcagcacat tcttt
//
LOCUS       DAZOZD010000511         1212 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZOZD010000511 DAZOZD010000000
VERSION     DAZOZD010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.519"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(13..285)
                     /locus_tag="ACHUPB_004727"
     CDS             complement(13..285)
                     /locus_tag="ACHUPB_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761289.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            complement(368..892)
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004728"
                     /pseudo
     CDS             complement(368..892)
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
BASE COUNT          314 a          270 c          304 g          324 t
ORIGIN      
        1 cggggctgaa aatcaccacc acttcgggtc atccgccttc atctccgctt ctgtttcgta
       61 taaatcaaaa cgacggcagg tatggcagaa cgtgacgtat tcgtacggtg aatccgggta
      121 cattttgtgg aaggtttccc agaaacagac cgtgcaggag ggatgaccgg cgataaagtc
      181 tgtaaatacg gactgataag ggtgattatt ggctctggcg acggctttca gaacctcttt
      241 caccattctg gtgtggactt tctggtgctc caggttgtgt gacatgggaa ctcattctgg
      301 atggttactc tgaaagctca tattctgcca caccccgatt tgcagccgcc aggctgccgt
      361 ggttcttacg ccagaaggga tttgtaactg gtctggtcgg ctccggcgta tcagtcagtg
      421 ccaccgggac aggcatgcat tcctgtatgt catcatcgct cagtaagccg tcctcgtact
      481 ggcttttcca tttaaacagc aggttatcat tgatatcgtg ttctctggcg atccgggcaa
      541 caacagcacc aggctgtaat gcctgcttag ccagacggac cttaaattca cggctatagc
      601 tggttcgccg ttcttttcgc catgagcctt ctctgatttg aggctctgtt aattccttct
      661 ttctgttggc ataaaggatg gcgtcaagtt gagcgaatga aactgaatcg ggcaatggcc
      721 atgcgatacc ggatgcaaga aatcgctgaa aaagcgtatg tattgtggaa tgactgagac
      781 ccagacgctg agcgatggcc cggatggtca gtttatcttc aaatcttaaa cgcagggcat
      841 caggcaaata agaacggaag cagggaatat cttttgttgt ctgggaattc atcgttcgtg
      901 tccatcaata tagatgggcg cgattgttgc cagacaggac aattttcaca agacgtcgct
      961 gatggggtgc ttacttataa gaggactcta tcatcgccaa tcatcagaca ttgaaataga
     1021 cgacactgag atgacgctta caattctttt ggtttctgta ataagtaatc tgacatcccc
     1081 cgtgcccaga agaggagagg gatatcaagc ccaagggtta ataattgata ctacggttaa
     1141 aattgccttc agtaaaggaa acgtgattaa attcagggga tttcagagtg aaaaccatac
     1201 aacatattaa aa
//
LOCUS       DAZOZD010000512         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZOZD010000512 DAZOZD010000000
VERSION     DAZOZD010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.520"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            166..>1211
                     /locus_tag="ACHUPB_004729"
     CDS             166..>1211
                     /locus_tag="ACHUPB_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0761290.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSILPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          391 a          276 c          193 g          351 t
ORIGIN      
        1 acatcaaaaa aagatgaacc aaatatacga aaaaagcaaa tatacttacc agggtagaaa
       61 gcatgatata acactcattc ccttatggcg aatattctga atcaccctcc tcattaggag
      121 agatacatta aatacaattt attcacaatc taattccata tccatatgcg agaaattaat
      181 atgctcagaa atatttcatc ctgtttattt ccacatatca gcacaattac atcccccaac
      241 cattatttgt ccgaatggga tgattgggag aaacaggggt taccggaaga acagcgtact
      301 gaggcggtaa gaagacttcg tgcatgtctt acctctaagg ggcataaact ggacctgcga
      361 gccttggcgc tttcctcgtt acctgtactc cctgcttgca ttaaaaagct tgatgtgagc
      421 tgtaataaat taaccatcct tactgattta cctgaaaata ttaaagaact tattgcaaga
      481 gataatttct taacacatat atctgcatta ccacattatc taataacttt ggatgtgtcc
      541 gaaaatcaat tagagaatct gccgttatta ccagacacca tcaaatcact aagcgcagag
      601 tataataggt tatccatact gccttcatta cccttgaatt taaaaaaact tgaggttagg
      661 aacaacgaac tgcaaactct tccatctctg ccttctaatc ttaagatact taaggttgcg
      721 cacaaccatc ttactgaact gcccccttta cctaggagac tgcaacttct ttttgcatat
      781 agcaatagat taagcaactt gccaaacatc caagaaaata ttatcatgag aagatttttt
      841 tattttgaaa acaaccaaat aactacaatc ccgacaaatc tttttcgttt agatcctcat
      901 ataactattg agattgcaaa taacccctta tcagatcaaa ctctgctatt cttaatacag
      961 caaacttcgg ttccaaattt taacgggcct cagtttcgta tttccctgtc agaccaaaac
     1021 agactgtttt tacgccagat gttgccgcaa aatttacatt cgcgccatat cagagtcatc
     1081 actgaagggg ggcagaactt tcagatcccc cctcttcccg aaactgtggc agcctggttt
     1141 cctgaagcag atcgtcggga ggtttctaca caatggactt ctttttccac cgaggagaat
     1201 tcccgggcat t
//
LOCUS       DAZOZD010000513         1211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZOZD010000513 DAZOZD010000000
VERSION     DAZOZD010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.521"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            54..1211
                     /locus_tag="ACHUPB_004730"
                     /pseudo
     CDS             54..1211
                     /locus_tag="ACHUPB_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          273 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca g
//
LOCUS       DAZOZD010000514         1207 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZOZD010000514 DAZOZD010000000
VERSION     DAZOZD010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.522"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            52..>1207
                     /locus_tag="ACHUPB_004731"
     CDS             join(52..301,301..>1207)
                     /locus_tag="ACHUPB_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0761291.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          343 a          294 c          332 g          238 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt cgcaagaaaa ccagtgggct gtgccatgtc gttctcgccg gacagcaggc
      841 tcaccatgaa agcgctggaa atggcatggg aaacccgtgg taagcccggc ggggtgatgt
      901 tccacagcga tcagggcagt cattatacga gcaggcagtt ccggcagtta ttgtggcgat
      961 accagatcag acagagtatg agccggcgcg gaaactgctg ggataacagc ccaatggaac
     1021 gcttcttcag gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg
     1081 aggcagctca cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg
     1141 aatataacgg tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact
     1201 cggtggc
//
LOCUS       DAZOZD010000515         1200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZOZD010000515 DAZOZD010000000
VERSION     DAZOZD010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.523"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..470)
                     /locus_tag="ACHUPB_004732"
     CDS             complement(<1..470)
                     /locus_tag="ACHUPB_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFR0761292.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSS"
     gene            complement(662..1045)
                     /gene="traM"
                     /locus_tag="ACHUPB_004733"
     CDS             complement(662..1045)
                     /gene="traM"
                     /locus_tag="ACHUPB_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR0761293.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
BASE COUNT          390 a          273 c          177 g          360 t
ORIGIN      
        1 gaagaatact catgactata acccaaaaga tacggtatta tcttattaaa gcgatatttg
       61 tcaggttcac aaccaaactc cttgcccttt ataaaaaagc cactaactac gtatctttca
      121 aagaatctcc agacaaacaa attcaagtta ttcacttccg tactttcaac caccacatcc
      181 catgatacat cattcaatat tacaccatta ataatactca ctccattcga ggacgagaaa
      241 gcatcaactt cagcagtatg aaataaaatt tgcgtatcaa tatcaatacc agaaaaccat
      301 atttcagaat cactattctg acactttatt aagtgtaaaa aaacagagtt tgagtctatg
      361 aattttcctg actctgtgcg gacacagact ggatattttg cggataaaat aatttccggc
      421 aggcctgatt gacgtatgag tgctctttct ctagtatcca tcgcacacat aggaacctcc
      481 tcaaaggatt ctatggacag tcgatgcagg gagggacaag ctccctgcat cggcgatttt
      541 caagttcaaa ttttaacgtg gcattaacca cgtaattaat atactaatta ttctgattat
      601 tgtcatcagt ttgaaccatt aaaaagtctg atatttgaac aaagtcaaaa tttatttccc
      661 tttattcatc atcatttttt ggaaagaacc tttccatctc agccgacacc ttctctctga
      721 tatcttcaac catattggca tattcaaact ttggatttcc tgagacatga ggactgagag
      781 actcaatacc caaaattttc gctactgatg actgtgtttt tacaacgcat tcaagaagta
      841 gtttattaaa ctcggcctga ttaaatgcag actctttgcg ctccatctga gcctcatata
      901 cacgaagacc cagttcaaga agcattgaag ccgttgctga aaaactgaca tctttttctc
      961 tcgccccctc ctgtcgacgt ttttcaataa tcgcgtttat tttttcataa gcatcattgc
     1021 tgatatacag attcacctta gccattagat attacctttt ttaaacataa aacaaagtta
     1081 atttcgaaac actagaattc atagtgcaaa cagggacgca ccgctagcag cgcccctgac
     1141 ggtatcctat aaaaaaacac accgcgccgc tagcagcacc cctaatataa aataatgttt
//
LOCUS       DAZOZD010000516         1198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZOZD010000516 DAZOZD010000000
VERSION     DAZOZD010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.524"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            223..1038
                     /gene="yahL"
                     /locus_tag="ACHUPB_004734"
     CDS             223..1038
                     /gene="yahL"
                     /locus_tag="ACHUPB_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR0761294.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          364 a          230 c          233 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagc
//
LOCUS       DAZOZD010000517         1185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZOZD010000517 DAZOZD010000000
VERSION     DAZOZD010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.525"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            215..>1185
                     /gene="cadB"
                     /locus_tag="ACHUPB_004735"
     CDS             215..>1185
                     /gene="cadB"
                     /locus_tag="ACHUPB_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0761295.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT          260 a          255 c          290 g          380 t
ORIGIN      
        1 gaatgtttat cttttcatga tatcaacttg cgatcctgat gtgttaataa aaaacctcaa
       61 gttctcactt acagaaactt ttgtgttatt tcacctaatc tttaggatta atcctttttt
      121 cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat agttatacat catgacccgg
      181 actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga agatcgggct
      241 atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg cattattacc
      301 tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct ctattattgg
      361 tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc aacaaggtgg
      421 cccaattgct tatgccggag aaatttctcc tgcatttggt tttcagacag gtgttcttta
      481 ttaccatgct aactggattg gtaacctagc gattggtatt accgctgtat cttatctttc
      541 caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta ttgctatcgt
      601 ctgggtattt acctttgtaa atatgctcgg cggtacctgg gtaagccgtt taaccactat
      661 tggtctggtg ctggttctta ttcctgtggt gatgactgct attgttggct ggcattggtt
      721 tgatgcggca acttatgcag ctaactggaa tactgcggat accactgatg gtcatgcgat
      781 cattaaaagt attctgctct acctgtgggc cttcgtgggt gttgagtccg cagcagtaag
      841 tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc tgggtactgg
      901 tttagcaggt attgtttaca tcgctgcgac tcaggtgctt tccggtatgt atccgtcttc
      961 tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct tcaactatcc tcggtaactg
     1021 ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc tgggctcctg
     1081 gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact tcccgaaagt
     1141 ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgct
//
LOCUS       DAZOZD010000518         1179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZOZD010000518 DAZOZD010000000
VERSION     DAZOZD010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.526"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUPB_004736"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="ACHUPB_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR0761296.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="ACHUPB_004737"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="ACHUPB_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          356 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctt
//
LOCUS       DAZOZD010000519         1177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZOZD010000519 DAZOZD010000000
VERSION     DAZOZD010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.527"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..103)
                     /locus_tag="ACHUPB_004738"
     CDS             complement(<1..103)
                     /locus_tag="ACHUPB_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761297.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSS"
     gene            complement(535..>1177)
                     /locus_tag="ACHUPB_004739"
     CDS             complement(535..>1177)
                     /locus_tag="ACHUPB_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR0761298.1"
                     /translation="SANERLKNNFNILYNQIRQYPAYYFKVASNVPNYSDICQFFSVM
                     YQGFQIVNHSGDVFIHACRENPQSKGDFVGDKFHISIAREQVPLAFQILSGLLFSEDS
                     PIDKWKITDMNRVSQQSRVGIGAQFTLYVKSDQECSQYSALLLHKIRQFIMCLESNLL
                     RSKIAPGEYPASDVRPEDWKYVSYRNELRSDRDGSERQEQMLREEPFYRLMIE"
BASE COUNT          371 a          233 c          219 g          354 t
ORIGIN      
        1 tagaacttgt tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt
       61 ttgttgaaaa tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag
      121 atgttataat actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag
      181 cttattcaga atgctcctat aagatagcga gctcgtcaac gatacagatg atctgattat
      241 attgtgcaaa aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca
      301 ttaactaatg caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag
      361 gagatgcacg ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt
      421 atgagccgaa tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc caaatctaat
      481 cgtagttatt ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc
      541 tatcatcaaa cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc
      601 tcgatcgctt cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc
      661 tgacgccgga tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat
      721 tataaattgt cgtattttat gaagtaataa agcactatat tgcgagcatt cctgatctga
      781 ttttacatat agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt
      841 catatcagtt atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa
      901 aatttggaat gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac
      961 aaaatccccc ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg
     1021 gttcacaatc tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaatt
     1081 tggcacgttc gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa
     1141 tatattgaag ttatttttca atctttcatt cgcagaa
//
LOCUS       DAZOZD010000520         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZOZD010000520 DAZOZD010000000
VERSION     DAZOZD010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.528"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(124..933)
                     /gene="pduF"
                     /locus_tag="ACHUPB_004740"
     CDS             complement(124..933)
                     /gene="pduF"
                     /locus_tag="ACHUPB_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR0761299.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT          394 a          283 c          230 g          255 t
ORIGIN      
        1 actgacgaga agcacaaagg acaataacaa aagcctccgt gaagcgggag actttttaat
       61 aagttcggaa agcagatcac aaacagtaaa aaaataaatg tttataactg ccagagtatt
      121 tcgttattcg ctaattcctt taggtgtaac actattgaca ggtaaattct ttgcaatgag
      181 atagcgataa atcgccgcac caacacatgc cccaatgatc ggtgcaataa tgggtacaat
      241 gaaataagga atattgcgtc caccagtcat cgcaatttct ccccaccccg caagccaggt
      301 gaaaatctta ggtccaaaat cacgcgctgg attcatggca aaaccggtta atggtccggt
      361 agaagcacca ataacagcaa ccagaatacc aataagtaaa ggtgcaagcg gtcctttggg
      421 tacgccatta ccgtcatcgg tcaacgccat aatcaacccc attaacatgg acgttatgac
      481 cacttcgacc aacgcagctt gccatacatt aatcgccgct gccggataag tactgaagat
      541 actggcgagt tgtaaacttt ctgcgctacc acgcaccata ttgtgagcag attcaaaatc
      601 ggtaaacaga ctactgtata gagaatatgc cagaactgca ccaccaaagg cacccgctaa
      661 ttgcgccaga caaaatggca gcactttacg ggctgggaaa gaggcaaaca gccacagagc
      721 aatggtaact gccgggttga gatgagcgcc ggaaatacct gccgtaaggt aaacagcaag
      781 cgaaatccct aacccccaaa tgatacaaat ttcccacagt cccagacttg ccccggcaac
      841 tttcagggcg cacaaacaac caatgccaaa aaagaggaac agtccggtgc ccaaaaactc
      901 ggcaacgcat tgcgccttga gtgaatcatt catagtgaca ccttttttac ggagtttttc
      961 tttatcagcc aactatgcgc tgactgttgt atataccgag cacaactata aaaaattgct
     1021 ctgcagaaaa tattgccaac atgcatgaca gactcgtgta aaaatcgtgc taacataaca
     1081 aattattgtc attaccgaat catgcaacac agcgttagtc agtgttgaaa gtaataattc
     1141 ccaccaaact tttacaataa aa
//
LOCUS       DAZOZD010000521         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZOZD010000521 DAZOZD010000000
VERSION     DAZOZD010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.529"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHUPB_004741"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHUPB_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR0761300.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1161)
                     /locus_tag="ACHUPB_004742"
     CDS             complement(510..>1161)
                     /locus_tag="ACHUPB_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0761301.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          276 g          257 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cgggggtcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa t
//
LOCUS       DAZOZD010000522         1159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZOZD010000522 DAZOZD010000000
VERSION     DAZOZD010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.530"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            25..>1159
                     /locus_tag="ACHUPB_004743"
     CDS             25..>1159
                     /locus_tag="ACHUPB_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFR0761302.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYLNEISIPLYTKMTDQDQLFVIKS"
BASE COUNT          350 a          199 c          236 g          374 t
ORIGIN      
        1 atcttttttg aaaaggtatt ttaaatgaaa aattttattc cttttgcgtt acctgaaatt
       61 ggcgaagaag aaattgcaga ggtaattgac tctttacgtt caggttggat tacgacaggt
      121 cctaaggcta agcaatttga acaagaattt tctaattacc taggagcgaa cgttcaatca
      181 ttagctgtta actctgctac gtcgggctta catttggctc ttgaagctgt tggcgtaaag
      241 ccgggagacc aagttattgt cccatcatat acattcactg ctactgccga aattgtcagg
      301 taccttggtg ctgatcctgt aattgttgat gtagatcgta aaacatttaa tatatcagtt
      361 gatgccattg agaaggctat tactaatgaa acaaaggcga ttattccagt acacttcgct
      421 ggattagctt gtgacatgga ttcaatctta tcaattgcta aaaaatatga cctaaaggtt
      481 gtcgaggatg ccgctcatgc atttcctaca acatataaag gaagtaagat aggaacgctt
      541 gattcagatg ctacggtttt tagcttctac gccaataaaa ctatgacaac cggtgaaggc
      601 ggaatggttg tttcaaaaaa taaagatata attgagcgtt gtaaggtaat gcgtttacat
      661 ggaatcagtc gtgacgcttt tgaccggtac cagtctaaaa ctccttcttg gttttatgag
      721 gttgtagctc cagggtttaa atacaatatg cctgatatct gtgcggcaat cggtattcat
      781 caacttagaa agatcgatga ttttcagaaa aaacgtcaac gaatggcaaa aatttacgat
      841 gatgcgttaa aagaattgcc acttgaattg cctgaatggc ctactaatgc tagtgatatt
      901 catgcttggc atctatatcc tatccgctta aaaactgatt cggctattaa tcgcgatgat
      961 tttattaaga agttatcaga tcttggaatt ggttgttctg tccattttat accgttgcat
     1021 aagcaaccgg tttggcgtga tacatataat ttgaacgcca gtgactttcc agtttctgag
     1081 gagtgttatt taaatgaaat atctattcct ctttatacta aaatgacgga tcaagatcag
     1141 ttgttcgtta tcaaatcga
//
LOCUS       DAZOZD010000523         1144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZOZD010000523 DAZOZD010000000
VERSION     DAZOZD010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.531"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            156..1100
                     /gene="leuO"
                     /locus_tag="ACHUPB_004744"
     CDS             156..1100
                     /gene="leuO"
                     /locus_tag="ACHUPB_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0761303.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          304 a          236 c          263 g          341 t
ORIGIN      
        1 attctgtttt tatcagaacc cgtatcttta tgttttccga attttactca ttttgctttt
       61 tcttatttta tatgcatgat aaatcatatt cttcaggatt atttctctgc attccaataa
      121 gggaaaggga gttaagtgtg acagtggagt taagtatgcc agaggtacaa acagatcatt
      181 cagagacggc ggagttaagc aagccacagc tacgcatggt cgatctcaac ttattaaccg
      241 ttttcgatgc cgtgatgcag gagcaaaaca ttacccgtgc cgctcatgtt ctgggtatgt
      301 cgcaacctgc ggtcagtaac gctgttgcac gcctgaaggt gatgtttaat gacgagcttt
      361 ttgttcgtta tggccgtggt attcaaccga ctgctcgcgc atttcaactt tttggctcag
      421 ttcgtcaggc attgcaacta gtacaaaatg aattgcctgg ttcaggtttt gaacccgcga
      481 gcagtgaacg tgtatttcat ctttgtgttt gcagcccgtt agacagcatt ctgacctcgc
      541 agatttataa tcacattgag cagattgcgc caaatataca tgttatgttc aagtcttcat
      601 taaatcagaa cactgaacat cagctgcgtt atcaggaaac ggagtttgtg attagttatg
      661 aagacttcca tcgtcctgaa tttaccagcg tgccattatt taaagatgaa atggtgctgg
      721 tagccagcaa aaatcatcca acaattaagg gcccgttact gaaacatgat gtttataacg
      781 aacaacatgc ggcggtttcg ctcgatcgtt tcgcgtcatt tagtcaacct tggtatgaca
      841 cggtagataa gcaagccagt atcgcgtatc agggcatggc aatgatgagc gtacttagcg
      901 tggtgtcgca aacgcatttg gtcgctattg cgccgcgttg gctggctgaa gagttcgctg
      961 aatccttaga attacaggta ttaccgctgc cgttaaaaca aaatagcaga acctgttatc
     1021 tctcctggca tgaagctgcc gggcgcgata aaggccatca gtggatggaa gagcaattag
     1081 tctcaatttg caaacgctaa ctgaatgcag aataggtcag acatgaatgt ctggtttatt
     1141 ctgt
//
LOCUS       DAZOZD010000524         1139 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZOZD010000524 DAZOZD010000000
VERSION     DAZOZD010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.532"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..113
                     /locus_tag="ACHUPB_004745"
     CDS             <1..113
                     /locus_tag="ACHUPB_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761304.1"
                     /translation="PVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            110..367
                     /locus_tag="ACHUPB_004746"
     CDS             110..367
                     /locus_tag="ACHUPB_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761305.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            367..741
                     /locus_tag="ACHUPB_004747"
     CDS             367..741
                     /locus_tag="ACHUPB_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0761306.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            738..>1139
                     /locus_tag="ACHUPB_004748"
     CDS             738..>1139
                     /locus_tag="ACHUPB_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0761307.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          323 a          249 c          316 g          251 t
ORIGIN      
        1 tgcctgtgga ccaggaaagt gcacgggtgc tttccactcc catgaagccc ggcaatccgc
       61 gcagatacag taataaacga actgacagca gtaagatgga gcgtttcttt tgaacaacca
      121 gacaatgact tttacccctg aacaattgcg taaacacgcg caggaaatgc tgcgtcatgc
      181 tgaacaactc gaaaaaacgg gcataacaaa agacgctatc cgtaaagata tggtacccgc
      241 gcttcgggaa ctgatgcagg cgaagcatcg cgcacagaaa gcagtagatg agctggtgga
      301 ctgtgtggca gagctggaaa ccagagtcgg aaagtttgaa aaactggtgc aggaggtgct
      361 gcgctgatgc gccatgagtt tattttacct tatccgccga cggtgaatac ttactggcga
      421 cgtcgtgaca acacatattt tgtatcaaaa gccggtgagc gttatcgccg ggatgtggcg
      481 cttattgtcc gtcagcagcg actgaaatta agcctgtccg gaagactggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa agcaccactg
      601 gatgcactga cacatgcggg agtgttaatg gacgatgagc agtttgatga aatcaatatc
      661 gttcgtggtc agccagtatc tggtggacgt ctgggggtga agatttaccc cataatgcat
      721 gaagagcagg tcaaaaaatg aaactggaag atttaccgaa atactactcc ccaaaatccc
      781 cttgcctgac cgatgcatcg gcctcaacgt caaaagatgc gctgagtatc actgatgtga
      841 tggccgcgca gggcatgaca cagaatcggg ctgagatggg gttttctgcg ttcctgggga
      901 aaatgggcat cagtatgaat gacagggcgc gggcaacaga attactggca gattatgcac
      961 tcagtcggtg cgatcgtgtg gcggcgttga gaaaacttcc ggcagaaata aaaccggtag
     1021 tgatgcgcat tatggcttcg tacgcttttg aggattatgc ccgcagcgca gcgagtaaaa
     1081 agcagtgccc ttgttgctat ggggaaaaat ttattgaaag cgtagttttt acaaacaag
//
LOCUS       DAZOZD010000525         1120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZOZD010000525 DAZOZD010000000
VERSION     DAZOZD010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.533"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(506..>1120)
                     /locus_tag="ACHUPB_004749"
     CDS             complement(506..>1120)
                     /locus_tag="ACHUPB_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0761308.1"
                     /translation="GYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIRNTT
                     GFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSL
                     ISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSA
                     IQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          354 a          215 c          193 g          358 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc
//
LOCUS       DAZOZD010000526         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZOZD010000526 DAZOZD010000000
VERSION     DAZOZD010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.534"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUPB_004750"
     CDS             84..1115
                     /locus_tag="ACHUPB_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0761309.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZOZD010000527         1108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZOZD010000527 DAZOZD010000000
VERSION     DAZOZD010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.535"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..660
                     /locus_tag="ACHUPB_004751"
     CDS             <1..660
                     /locus_tag="ACHUPB_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain-length determining protein"
                     /protein_id="HFR0761310.1"
                     /translation="LAEQANKGLVITPPKEKGNTSYYIIQASADSAQEAYKLLQGYLK
                     NVNNQAVTLSLDEFGQNVNTLLVNLNKEIIDIDFQRKSEKLDQIAHIQRDLTTAEQAG
                     IIDYRSSKGGFDNAQSSYKFLLGEKLLSAELKATKDAPIIYPFRYYEVKRQIDELEGM
                     LRDNIQAQAYRYQMKPSEPVIKDKPNKALILILGALPGAMFAIVGTLVYATLKDKTKL
                     D"
     gene            729..>1108
                     /locus_tag="ACHUPB_004752"
     CDS             729..>1108
                     /locus_tag="ACHUPB_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HFR0761311.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGA"
BASE COUNT          396 a          170 c          225 g          317 t
ORIGIN      
        1 ttagctgagc aagcgaataa aggtcttgtg attacgccac caaaggaaaa gggaaataca
       61 agttactaca taatacaagc atcggcagac tcagcgcaag aggcatataa actactgcag
      121 ggatatctaa agaatgttaa taatcaagct gtaacattaa gtcttgatga gtttggtcaa
      181 aatgttaata ctcttttggt taatctaaat aaagaaatta ttgacataga tttccagaga
      241 aaatcagaaa agcttgatca aatagctcat attcagcgag atttaacaac tgcggaacaa
      301 gccggaatca ttgattatcg ctctagcaaa ggcggcttcg ataatgcgca aagtagctat
      361 aagttcttgc tcggcgaaaa actgttatca gcagagctaa aagcaactaa agatgcgcca
      421 attatttacc catttagata ttacgaagtg aaacgtcaaa ttgatgagtt agaaggaatg
      481 ttacgcgata acattcaggc gcaagcatat cgatatcaaa tgaagccatc tgagccagtt
      541 ataaaagaca aacccaacaa agcattaatt ttgattcttg gtgcattacc aggggcaatg
      601 tttgctatag ttggtacatt agtttatgcg acattaaaag ataaaaccaa gttagattaa
      661 actgggttac gtattgttgt gtcaatgcga aatagatgtt ctatgtgcac tttatgatgg
      721 ataagaaaat gaaattcgat actttgaatg cgaaaattgg gattataggc cttggttatg
      781 ttggattgcc tcttgctgtt gagtttggaa agaaagtaac gacgattgga tttgatatta
      841 ataagtctcg tattgatgaa ttacgaaatg gtcacgatag tacattagag tgctcaaatt
      901 tagagttgtt agaagcaact aaattgacgt acgcctgttc attagatgca ctaaaagagt
      961 gtaatgtatt tattgtaact gttccaactc caattgataa acataaacag ccagatctaa
     1021 cacctctaat taaagcatct gaaacattgg gtaagataat aaagaaaggc gatgttatta
     1081 tttatgagtc aacagtttac cctggagc
//
LOCUS       DAZOZD010000528         1107 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZOZD010000528 DAZOZD010000000
VERSION     DAZOZD010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.536"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            295..927
                     /locus_tag="ACHUPB_004753"
     CDS             295..927
                     /locus_tag="ACHUPB_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0761312.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          384 a          191 c          201 g          331 t
ORIGIN      
        1 atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt gcgatataga
       61 tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga ttaaatcagt
      121 gaaacataat catatttaat aacgagaaat gcatttttaa atgtcttaat cagaattata
      181 gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaaga tgtgttgaca
      241 tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct taacatgggg
      301 ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg ccaaataatg
      361 ccagaagagt caccgcgttt gcagtattgc gcaaactcgt gtttttgtat gtggcccgaa
      421 gaaagttcat attttaatcg tggtgtggtg gaaggtatct taacaaaaaa ccacaatgcg
      481 agattaagcg gatatatttt tgtggatttt tcagtaagtt ttttacgtct attccttgaa
      541 aaagactgga ttgactatct tgcaagtact gacatgggga tagttttagt cagcgaccgc
      601 aatatgcaat cgttagctaa ctactggcga aaacacaaca ccgcaatatc tgctgtcata
      661 tataatgatg atggtcttga tgttgccaat gaaaagatca gacaactatt tattggtcgt
      721 tatttatcat ttaccagagg taacacatta actcagatgg aattcaccat aatggggtat
      781 atggtttctg gttataatcc atatcagatt gctgaagtgc tggatatgga tatccgtagc
      841 atctacgcgt acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa cgaattattt
      901 attcgttcac attcggtcca acattgatac tcaacgacca gccagaatca tactctgttc
      961 atacgggcat ctattgaaat gcccgtattt tataaataag cagtaacccc aagagtaaac
     1021 aacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat atgaatttac
     1081 aaacaatagt ggcataaatg ttaacca
//
LOCUS       DAZOZD010000529         1106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZOZD010000529 DAZOZD010000000
VERSION     DAZOZD010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.537"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..812
                     /gene="gadX"
                     /locus_tag="ACHUPB_004754"
     CDS             <1..812
                     /gene="gadX"
                     /locus_tag="ACHUPB_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0761313.1"
                     /translation="GNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVE
                     NFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLR
                     YAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIA
                     SELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSY
                     FIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          324 a          206 c          237 g          339 t
ORIGIN      
        1 atgggaattg tctaattgcg tacgcaagac ataaatatat tctcaccatg gttaatggtg
       61 aatatcgcta ttttaatggc ggtgacctgg tttttgcgga tgcaagccaa attcgagtag
      121 ataagtgtgt tgaaaatttt gtattagtat caagggatac gctttcatta tttctgccga
      181 tgctcaagga ggaggcatta aatcttcatg cacataaaaa aatttcttca ttactcgttc
      241 atcactgtag cagagatatt cctgtttttc aggaagttgc gcaactatcg cagaataaga
      301 atcttcgcta tgcagaaatg ctacgtaaaa gagcattaat ctttgcgttg ttatctgttt
      361 ttcttgagga tgagcacttt ataccgctgc ttttgaacgt tttgcaaccg aacatgcgaa
      421 cacgagtttg tacggttatc aataataata tcgcccatga gtggacacta gcccgaatcg
      481 ccagcgagct gttgatgagt ccaagcctgt taaagaaaaa attgcgcgaa gaagagacat
      541 catattcaca gttgcttact gagtgtagaa tgcaacgtgc tttgcaactt attgttatac
      601 atggcttttc aattaagcga gtcgcagtgt cctgtggata tcacagcgtg tcgtatttca
      661 tttacgtctt tcgaaattat tatgggatga cgcccacaga gtatcaggag cgatcggcgc
      721 agggattgcc gaaccgtgac tcggcggcaa gtattgttgc gcaaggaaat ttttacggca
      781 ctgaccgttc tgcggaagga ataagattat agaattttac tcagacataa aaaaaacccg
      841 gcatagggga ccgggaagag gatagtctgc cgtctccaga ctaataaacc gttataacac
      901 tccctgttgg cacgggaaac tttgtgctct cagtaagtta aatataactt ttactggaaa
      961 taagatcagc cattttttta taaacataag ctatacgctg tgcgaaaata tattcgtgct
     1021 gcatttactt attatcaatt aactgttatg caaaactact ttgtggataa attttggtcc
     1081 taccaaatct ggcagttttt tcgcta
//
LOCUS       DAZOZD010000530         1104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZOZD010000530 DAZOZD010000000
VERSION     DAZOZD010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.538"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..408
                     /locus_tag="ACHUPB_004755"
     CDS             <1..408
                     /locus_tag="ACHUPB_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR0761314.1"
                     /translation="KKKKRHRFPRPSHWTREYTHECVEKIKILFPHLRAEGGGFIPLK
                     IGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDGHPKGQ
                     VSESEAQSAGRRLVTLEQKLLRMQAQQENISGQ"
     gene            464..972
                     /locus_tag="ACHUPB_004756"
                     /pseudo
     CDS             464..972
                     /locus_tag="ACHUPB_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          345 a          229 c          253 g          277 t
ORIGIN      
        1 aagaaaaaga aaagacaccg ttttccacgt ccctcccact ggaccagaga gtatactcac
       61 gagtgcgtgg aaaaaataaa gatcctgttt cctcatcttc gtgctgaagg tggtggattt
      121 attcccctga aaattggcat aaacaatgat atttcagcat ttctggcaga acacccggag
      181 acagaactga ccatggacga atggctctgt gcagtgtcct gcatcacctc acgccgtgta
      241 tatctgcagc ggacagctgt cgccggcgtc ccacgttatg gtctggatgg tcaccccaaa
      301 gggcaggtct cagaatctga agcacagagt gccggtcgca gactggtgac attagaacaa
      361 aagttgctca ggatgcaggc acagcaggag aatatttctg ggcaataaac actcctgata
      421 acagctggac attacgtatc agaaaaatgg gaagagaatt attatgagta aatcaggtgc
      481 agaaaaaatt caattcatcc tgaataatca tgaacaggca tacagacgcc tgaagcctgt
      541 atatactttt ttagaactgg catcctggat ttcggttgtt ctgctgatta tatacctatc
      601 aggcaagaat cacgtgctta cagcagcttt tctctgcttt gcatggacag cattctgggt
      661 tcttttcctt ttacattatt tcccaaaagt tttctttatt ctcgatcgtc agagtctgct
      721 caacctcatg gaggccacca aagactcacc agatgtaaag caggagctgc ttaaccgcct
      781 tttttccgga aaaagaatga ccgggcggga tgaaatggat atctgccgtc ttctgagaaa
      841 aatgaagaag aaaaaatccg ccagagtgaa ctgaatatca tcaggaattt tattgatgaa
      901 cagcagttaa cgaatagtga tattccgaag aaaagtacgg tcccaaatga agataaaaaa
      961 ggcataagat gatatgaaat taataaccag gcaagcagag gatggcgtgg tttcatccta
     1021 agattgatag gagttaacct gtaagataaa aggaccgtgt ttacacgacg tctctgaagg
     1081 gcttcgtttg ggaaacggac aata
//
LOCUS       DAZOZD010000531         1098 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZOZD010000531 DAZOZD010000000
VERSION     DAZOZD010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.539"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..283)
                     /locus_tag="ACHUPB_004757"
     CDS             complement(<1..283)
                     /locus_tag="ACHUPB_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR0761315.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYT"
     gene            complement(300..>1098)
                     /locus_tag="ACHUPB_004758"
     CDS             complement(300..>1098)
                     /locus_tag="ACHUPB_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0761316.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          291 a          251 c          222 g          334 t
ORIGIN      
        1 atgtatagga ttcggaaagt ccagcatcta ttttgtacct cattatatta gataaggatg
       61 tgagaaattc cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg
      121 ccttacaggc aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata
      181 ttagtgccca taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag
      241 taactgcact cgcagaaata gagaaaaaaa tacaattgtt aataactttg ccatattttt
      301 tagtccgtaa ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc
      361 atgtcattag atgaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga
      421 ccgaaagcgt ttaaacaact ttgtgttaaa aatccatcac gattatatgt ttctgtattt
      481 gctgacggta ctaaacgtat accattaggt tttctattgg ttgctccagg accattaaca
      541 ttccagctcc ataccccttg aggtattgga cctctatttt gtaagtgtgt acaaccagga
      601 ttatttttac attgcatatt attaccatta ttaccagagg cgacaggaat agtaagcaca
      661 tcataagagt tatcatcatt ttgcgtatct gaaacgcatg aaagcatact taaagttatg
      721 ctgtataaac aggtcgctaa tcccaacggg tcaatatcag ttatcggatt caacggatac
      781 tgataaaaat tccatccccc cttcagtcca atcggatcct gagtgatata tcgcccctgc
      841 agcgggtcat aatagcggtg gcggttgtaa tacaggccgg actcctcatc atactgctgc
      901 cccggcaggc ggataagctg ctgcagctga tgcgggttct cttcattcag caggttgccc
      961 cactcatcgt attctgcgca ccattctgtt gccccttccg tgctgataag caccagcggc
     1021 aggccgcgat ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta caccgggtcc
     1081 atctggtttt gcatctgc
//
LOCUS       DAZOZD010000532         1096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZOZD010000532 DAZOZD010000000
VERSION     DAZOZD010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.540"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..1074
                     /locus_tag="ACHUPB_004759"
     CDS             join(<1..167,167..1074)
                     /locus_tag="ACHUPB_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFR0761317.1"
                     /translation="EGASRLSLPEGTLGQWVTAARKGLGTPGSRTVAELESEILQLRK
                     ALNEARLERDIFKKSNSVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQ
                     HEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRL
                     RCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIK
                     DVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQE
                     QSGLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQ
                     RRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          312 a          261 c          286 g          237 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtctggtctg
      841 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
      901 ggaacgctga aaaatgagag cctgagccac tatcgtttta ataaccggga tgaagccatc
      961 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     1021 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag
     1081 aacaaatggt agtgtc
//
LOCUS       DAZOZD010000533         1095 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZOZD010000533 DAZOZD010000000
VERSION     DAZOZD010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.541"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            418..852
                     /locus_tag="ACHUPB_004760"
     CDS             418..852
                     /locus_tag="ACHUPB_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFR0761318.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPSGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
BASE COUNT          222 a          335 c          321 g          217 t
ORIGIN      
        1 aaaccaggct tgcagcgacc gttccggtcg cgccgcctct gtcgcgccgt gacctgcgct
       61 ccctgcacac ctgacggtgt gtaaggaaac tgcgcctgat ggcgcagttg tctgcccttc
      121 cgccgccacc cctgcggcct gtttcccgct gtggactttc cgggggctga agcggccaca
      181 acacgtgtca gccgtccagg ggggagatgt accagccgca aaactttttg ccgcaagcgt
      241 caaaaacttt tgcgtctgcc cccggacaac tgccccgtgc cgtggctgtc gctgccccat
      301 cggaaaggtc cactgcgggg agaaaccccg aagaagtgga cggcagaagg ggctttcgcc
      361 cctccgggga gcaaccggtc ggcccggcga cctgattcag cagaaggaga cgttttcatg
      421 acagtcagca gcacgatttc cgttttttgc cgcgacgggg tgttccgtac cgtttactgc
      481 cacctgcacg gtgagccgac ctggaacggt cgcatcctgc atacccacta tgccactggt
      541 cagcaggccg aagccctggt tgaacacggt gatatccgtt gcctcggtcc ccgttgcgac
      601 aaaccctccg ggcatacgct tcagaacccg gtggacggtg tgacggctta ttacggacgt
      661 gacagtggtt tccggatgga cagtgaggcg cgtgagtacc gttctttcat ggaggctatt
      721 gccactgaaa gcactgaaga ggtgcgcttc cattatgtgt tcatcgacgg ctactggaag
      781 gtgatgtatc gcacgccgga aggctggaag atgaaagcgc tcgcgctggc actgcgccgc
      841 tgtccgaaat gaaaaaaagg cagggcgata aaccctgcct ctctccgccg gcgcttcccc
      901 gccaggaagc ccggcatctc aatcactatc tatggagatt atgccatgag accatcaatt
      961 atcttcgcaa ccgccgagta tgtaaagcgt ctgcgtgaag agtgcctgcg ggagaataaa
     1021 cccctgcacc gccatacccg cttcagacgt caggagctgg cacaggatga gattaacccg
     1081 gacgtcctgg cgatg
//
LOCUS       DAZOZD010000534         1071 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZOZD010000534 DAZOZD010000000
VERSION     DAZOZD010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.542"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            230..358
                     /locus_tag="ACHUPB_004761"
                     /pseudo
     CDS             230..358
                     /locus_tag="ACHUPB_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            548..952
                     /locus_tag="ACHUPB_004762"
     CDS             548..952
                     /locus_tag="ACHUPB_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761319.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          325 a          238 c          266 g          242 t
ORIGIN      
        1 cctctcccac aaagtgaatt aaatgaatat ataatgcgtt cgaagtatat gtattgagct
       61 gtcatactga gaaacaaacc ttagggcagc acaattaaag ccagaaacaa ctatgcaaga
      121 aagttactaa ataagtgctc ggaacagtaa aacactcagg ggggactcag tccggattat
      181 gtaatcagat aaattgccaa acatcacaaa tcaccagccg gactgagtga tgccgagcat
      241 tgcaccaata ccccgtgatg aacgatgcct gatgcagaaa gctccctata aaacaaagaa
      301 caaaaactac gaccggcgat tcaccgtcat actgatgctg catcgtggtg acactgtctg
      361 tcactttcat gttgactgaa caactctgct gcgaagaagg tgtaccgccg gcgggatttg
      421 attgtccggc gtctagcttc cacttccctg atggaactga aagttgttat ggccggtcag
      481 cttgccctgt ccgcaggaca tgaggtgctg tgacggacag caccagtaac ggaaagtgac
      541 aatgaaaatg aatactgcgc tgatagttgc actaatgtgt atgtggtatg cggtacccgc
      601 tgctgcaaaa gagactttac tggctatgcc acgtaattca acagaacatt gctatgcgga
      661 gattaatgtg cacggaccat atggggtgta tttccgagtg gtcccgcatc cccccggggg
      721 aaagagctgg gtcgaatgta acagtgacta ctattactct gataaaccac cgggagtaca
      781 gatactcgga acccgtgcag ggtgtcgggt atacggaatc tgcgggacca cctcgacact
      841 gcatgtggct ggaagagggg ttgtgtgtat taaaaatata tgcagtccac gtgggatgat
      901 aattcacagg atacggaaac gtcctgtagt ggctgtaagc gatgagatgt gatcttaccc
      961 agcaatagtg gacacgcggc taagtgagta aactctcagt cagaggtgac tcacatgaca
     1021 aaaacagtat caaccagcaa aaaaacacgt aaacagcatt cgcctgaatt t
//
LOCUS       DAZOZD010000535         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZOZD010000535 DAZOZD010000000
VERSION     DAZOZD010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.543"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            97..1035
                     /gene="mdh"
                     /locus_tag="ACHUPB_004763"
     CDS             97..1035
                     /gene="mdh"
                     /locus_tag="ACHUPB_004763"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0761320.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          264 a          244 c          294 g          268 t
ORIGIN      
        1 cttgagatgt ggtcattgta aacggcaatt ttgtggatta aggtcgcggc agcggagcaa
       61 catatcttag tttatcaata taataaggag tttaggatga aagtcgcagt cctcggcgct
      121 gctggcggta ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc ttcaggttca
      181 gaactctctc tgtatgatat cgctccagtg actcccggtg tggctgtcga tctgagccat
      241 atccctactg ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc ggcgctggaa
      301 ggcgcagatg tcgttcttat ctctgcaggt gtagcgcgta aaccgggtat ggatcgttcc
      361 gacctgttta acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt tgcgaaaacc
      421 tgcccgaaag cgtgcattgg tattatcact aacccggtta acaccacagt tgcgattgct
      481 gctgaagtgc tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg cgttaccacg
      541 ctggacatca ttcgttccaa cacttttgtt gcggaactga aaggcaaaca gccaggcgaa
      601 gttgaagtgc cggttattgg cggtcactct ggtgttacca ttctgccgct gctgtcacag
      661 gttcctggcg ttagttttac cgagcaggaa gtggctgatc tgaccaaacg catccagaac
      721 gcgggtactg aagtggttga agcgaaagcc ggtggcgggt ctgcaaccct gtctatgggc
      781 caggcagctg cacgttttgg tctgtctctg gttcgtgcac tgcagggcga acaaggcgtt
      841 gtcgaatgtg cctacgttga aggcgatggt cagtacgccc gtttcttctc tcaaccgctg
      901 ctgctgggta aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag cgcatttgaa
      961 cagaacgcgc tggaaggtat gctggataca ctcaagaaag atatcgcctt gggcgaagag
     1021 ttcgttaata agtaattgat tagcgaataa taaaaaaccg gagcacagac
//
LOCUS       DAZOZD010000536         1067 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZOZD010000536 DAZOZD010000000
VERSION     DAZOZD010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.544"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(270..992)
                     /locus_tag="ACHUPB_004764"
     CDS             complement(270..992)
                     /locus_tag="ACHUPB_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0761321.1"
                     /translation="MLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRARLLKQYQ
                     THLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLS
                     SDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANE
                     MYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRL
                     AEPFWQDHFLGARRILTEETIL"
BASE COUNT          271 a          268 c          267 g          261 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggccc
//
LOCUS       DAZOZD010000537         1064 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZOZD010000537 DAZOZD010000000
VERSION     DAZOZD010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.545"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..292)
                     /gene="mxiC"
                     /locus_tag="ACHUPB_004765"
     CDS             complement(<1..292)
                     /gene="mxiC"
                     /locus_tag="ACHUPB_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0761322.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTL"
     gene            complement(311..>1064)
                     /gene="mxiD"
                     /locus_tag="ACHUPB_004766"
     CDS             complement(311..>1064)
                     /gene="mxiD"
                     /locus_tag="ACHUPB_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR0761323.1"
                     /translation="NLGVNWQGTASFGDSFGASFNMSSSASISTLDGNKFIASVMALN
                     QKKKANVVSRPVILTQENIPAIFDNNRTFYVSLVGERNSSLEHVTYGTLINVIPRFSS
                     RGQIEMSLTIEDGTGNSQSNYNYNNENTSVLPEVGRTKISTIARVPQGKSLLIGGYTH
                     ETNSNEIVSIPFLSSIPVIGNVFKYKTSNISNIVRVFLIQPREIKESSYYNTAEYKSL
                     ISEREIQKTTQIIPSETTLLEDEKSLVSYLNY"
BASE COUNT          324 a          219 c          157 g          364 t
ORIGIN      
        1 ttaacgttct ctttaaatca aaaatcttgt gattaatttc atcttcttcc ccatctaaaa
       61 tacgttcctg actatcacta tttgttcctt tcagtttctc aaggtctctt ctgtttataa
      121 atgacgaaag agcggaagcc atctcatcag atgagtcaat atacttgcta ttagccaagc
      181 cagaatcaag ctctgcatca gcagtctcat ctccatcatc tgaatttgtc attgcattca
      241 acttatcaat gaatgcagag ctaaaaactc ctgtattttt aacatcaagc atacatcacc
      301 tactttatag ttagtaattt aagtatgaaa ccaatgattt ttcgtcttcc agcaaagtag
      361 tttcagatgg gattatttgt gtagtctttt gtatttctct ttcagatatg aggcttttat
      421 attctgcagt attataataa cttgactcct ttatctcacg gggttgaatc agaaaaaccc
      481 ttacaatatt actgatatta cttgttttat acttgaacac attaccgatt acaggaatag
      541 aggagagaaa aggaatagat actatttcgt tagagttcgt ttcatgcgta taaccaccta
      601 tcaacaaact tttgccttgt ggaactctcg ctattgtact gattttggtc cttccaactt
      661 ctggaaggac agatgtattt tcattattat aattgtaatt tgactgacta tttcctgttc
      721 catcctctat cgttaaagac atctcaattt gtccccgaga tgaaaagcga ggtataacat
      781 ttatcaatgt tccatacgta acatgctcca acgaagaatt gcgctcacca actaatgaca
      841 cataaaaagt cctgttatta tcaaatatag ccggaatatt ctcttgtgtc aaaatcaccg
      901 gccttgaaac tacatttgct ttctttttct gatttaatgc cataactgaa gcaataaact
      961 tattgccgtc caaagtgctt atcgatgctg atgagctcat attaaatgac gcaccaaacg
     1021 aatcaccaaa tgaagccgtt ccttgccaat taacaccaag gtta
//
LOCUS       DAZOZD010000538         1059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZOZD010000538 DAZOZD010000000
VERSION     DAZOZD010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.546"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUPB_004767"
     CDS             complement(<1..906)
                     /locus_tag="ACHUPB_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR0761324.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          302 a          239 c          210 g          308 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataa
//
LOCUS       DAZOZD010000539         1059 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZOZD010000539 DAZOZD010000000
VERSION     DAZOZD010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.547"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>1059)
                     /locus_tag="ACHUPB_004768"
     CDS             complement(<1..>1059)
                     /locus_tag="ACHUPB_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0761325.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDE"
BASE COUNT          164 a          409 c          294 g          192 t
ORIGIN      
        1 ctcatcatac tcccggctgc tgcgaagccc gtccgggtac gtcaccgacg tcacctgccg
       61 ctggctgtta tagccatacc gtaccgtcct gccgtccggc agtatcactg aagtcagctt
      121 tccagacgcc atatgcagtc ggtactccgt ccgccgtccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cagctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgcacctg
      481 catcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaagga
      661 aggagctgag agagggcagg gactgtcagg ggaagataac gaagcggtgt gcggtttacg
      721 ggcctcttcc gcccgctgcg cctgtgtggt cagcaccagg tgaaaacggc ggcccgcgcc
      781 gtccatcacc cccgtcaccg cgcccgccac gtcaccctcc gctgcccggt gaaaggccag
      841 cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg gctccggcgg
      901 cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc acggcccctg
      961 aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg gcagcacctg
     1021 ccagagcgcg cttagcggct gcgaactgtg ctgcgccgc
//
LOCUS       DAZOZD010000540         1055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZOZD010000540 DAZOZD010000000
VERSION     DAZOZD010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.548"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            9..794
                     /locus_tag="ACHUPB_004769"
     CDS             9..794
                     /locus_tag="ACHUPB_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761326.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(931..>1055)
                     /gene="ykfM"
                     /locus_tag="ACHUPB_004770"
     CDS             complement(931..>1055)
                     /gene="ykfM"
                     /locus_tag="ACHUPB_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR0761327.1"
                     /translation="NANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
BASE COUNT          344 a          191 c          225 g          295 t
ORIGIN      
        1 gtccgattat gaatttaaga agggtttgtt gtatatgtgt attattctcg ctgcttgcag
       61 gatgtgtctc tgaatcttct attgatgaaa agaagaaaaa agctcaagtc acacaaagta
      121 atattaataa aaacactccc cagcaactga cagacaaaga tttattcggt aatgaaacca
      181 ctctggccgt atccgaagag gatattcaag ctgcgcttga tggagatgag ttccgcgttc
      241 cccttaattc tcctgtaata cttgttcaat ccggcaaccg cgcaccggaa accattatgc
      301 aggaggagat gcgaaaatat tatactgttt ccacattctc tggtatcccg gacaggcaaa
      361 agcctctgac ttgtaacaaa aacaaagata aaaatgagaa cgaggatgtt gccagtgctg
      421 agaatatgaa ctggatgcag gcactgcgtt ttgtggctgc aaaagggcat cagaaagcga
      481 ttattgtttt ccaggatacg ttgcagacag gaaaatatga ctctggattg aaatcaatag
      541 tttggtcaga ttataaaaat caaaaattga ctgacacgat gtcactgcgc tatctggtac
      601 gattcacgct ggtggatgta gctacaggtg agtgggctgc ctggtcgccg gtgaattatg
      661 aatataaagt gcttccacca ttgcccgaca agaatgaagc cagtacgact gatatgacag
      721 agcagcaaat catgcaactt aagcaaaaga cctataaagc gatggtaaaa gatttggtta
      781 accgctatca ataataaatt atatctgccg ccaggaattt ctggcggaaa taatacaaaa
      841 ttctttgcat aagaaatttt tcttgacaaa gacagtgcaa gaaaggacat ggaatccgga
      901 gttaaaaact gtttgtttta gaatgtttaa ttatttaact gctgaatctt ccttgcgaga
      961 aatatattca ttattacatt tcatgccatt tttaatatag attgctcctg ggaaaattcc
     1021 cgtattatta ccgcaggggt gataattagc attga
//
LOCUS       DAZOZD010000541         1049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZOZD010000541 DAZOZD010000000
VERSION     DAZOZD010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.549"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..881
                     /gene="lpxM"
                     /locus_tag="ACHUPB_004771"
                     /gene_synonym="msbB"
     CDS             <1..881
                     /gene="lpxM"
                     /locus_tag="ACHUPB_004771"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0761328.1"
                     /translation="FTWFVLGMIAGISMFPPSFRDPVLAKIGRWVGRLSRKARRRATI
                     NLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELAIRGPEKFQKRVFWKGLEILEEI
                     RHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFHQQRNPVIDYVWNSVRRKFGGRL
                     HSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKATLPIIGRLMNISQA
                     MIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMIARQMNKTVEILVGSHPEQYIWV
                     LKLLKTRKSNEADPYP"
BASE COUNT          293 a          210 c          253 g          293 t
ORIGIN      
        1 ggtttacatg gttcgttttg ggaatgattg caggtatttc aatgtttccc ccttcattca
       61 gagatcctgt cttggccaaa atagggcgtt gggtgggcag attgagcaga aaagctcgtc
      121 gcagggcgac gattaattta tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa
      181 taattgtcga caatatgttt gccacagcat tgcaatccat agtgatgatg gcagaactgg
      241 caattcgtgg tccggaaaag ttccagaaac gagtgttctg gaaaggcctc gaaattcttg
      301 aggaaatccg gcacaataac agaaatgtga tttttctggt tccccatggc tggagcgtgg
      361 atattcctgc aatgttgctg gcagctcagg gggaaaaaat ggccgccatg tttcatcagc
      421 aacgaaatcc agtgattgat tatgtctgga attctgtacg gcgtaaattc ggggggcgct
      481 tacattcccg ggaggatggg ataaaaccat ttattcagtc agtacgccag ggatactggg
      541 ggtattacct tccagatcag gatcatggtc ctgaatacag tgaatttgct gatttttttg
      601 cgacctataa agcgacatta ccaattattg gacgtctgat gaacatcagt caggctatga
      661 ttataccgct tttcccggtt tatgatgaaa aaaaacattt cctgactatt gaagttcggc
      721 caccaatgga tgcatgcatt gccagcgcgg acaataaaat gattgcccga caaatgaaca
      781 aaacagtgga aattttggtg gggtcacatc cggaacagta tatctgggtt ttaaaattgt
      841 taaaaacgcg caaatcaaac gaagcggacc cgtacccttg aaattataaa gtacaggtat
      901 ttccactagt tgtttcttac aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc
      961 cgtttggcac ggaggactgt gtagatgcag ttcgttaaat ttaatttatg cctaaatagc
     1021 tgcgcttaat accgctacac ttttgccag
//
LOCUS       DAZOZD010000542         1046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZOZD010000542 DAZOZD010000000
VERSION     DAZOZD010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.550"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..1046
                     /locus_tag="ACHUPB_004772"
                     /pseudo
     CDS             1..1046
                     /locus_tag="ACHUPB_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          262 a          307 c          314 g          163 t
ORIGIN      
        1 aacccggcag gcttaagcta cacgtatcag tatgagaagg accgcatcac catcaccgac
       61 agcctgaacc gccgtgaagt gctgcacacg tagggtgaag ccgggctgaa gcgggtggtg
      121 aaaaaggaac acgcggacgg cagcgtcacg cagagtcagt ttgacgccgt gggcaggctc
      181 aggacacaga cggatgccgc aggccggaca acagaataca gcccggatgt ggtgacgggc
      241 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
      301 agccagttaa cgtcagccac cgggcctgac gggctggaaa tgcgccgcaa atatgatgaa
      361 tccggccgtc tgattcagga aaccgcccct gacggcgata tcacccgcta ccgttatgat
      421 aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg gaaaaccatg
      481 acatggagcc gttacggtca gttgctgacc gtcactgact gttccggtta tgtaacccgt
      541 tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg gctgagccag
      601 taccgcgcat acgacagccg tggacagtta attgcggtga aagacacgca gggccatgaa
      661 atgcggtatg aatacaacgc cgccggtgac ctgaccgccg tcattgcccc ggacggcagc
      721 agaaacggga cacagtacga tgcgtgggga aaagccatct gtaccacgca gggcgggctg
      781 acgcgcagta tggaatacga tgctgccgga cgggtcatcc gtctgaccag tgaaaacggc
      841 agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac cggctttgac
      901 ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag cgaggatgag
      961 gggctggtca cccactggca ctatgacgaa gcagaccgcc tcacgcaccg caccgtgaag
     1021 ggtgaaaccg cagagcggtg gcggta
//
LOCUS       DAZOZD010000543         1045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZOZD010000543 DAZOZD010000000
VERSION     DAZOZD010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.551"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            156..1045
                     /locus_tag="ACHUPB_004773"
                     /pseudo
     CDS             156..1045
                     /locus_tag="ACHUPB_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT          290 a          254 c          269 g          232 t
ORIGIN      
        1 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
       61 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
      121 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
      181 gcgcagccct gggagtaaat gcgccccctg atcactgttg aggtacatat cagtaaaggt
      241 ctacccggct taacgatggt gggcttacca gaaacaactg taaaagaagc tcgcgatcgc
      301 gtgcgcagcg ccattatcaa tagcggatat gaatatccgg cgaaaaaaat caccatcaac
      361 cttgctccag ccgatctgcc aaaagaaggg ggacgatatg atttacctat cgccattgcg
      421 ttgctggcgg cctcagaaca gcttacagcc aataagttag atgaatatga attagtcgga
      481 gaactggctc ttacaggcgc tctgcgtggc gttcccggtg caatctccag tgcaactgaa
      541 gctattaagt cgggcagaaa aattatcgtc gcgaaagata acgaagatga agtggggcta
      601 attaacggtg aaggatgcct gatagccgat catctgcaag ctgtctgtgc gtttctggaa
      661 ggtaagcacg ctctcgaacg cccgaaacca actgatgcag tatcccgggc gctacaacat
      721 gatctcagtg atgttgtcgg tcaggaacaa ggaaagcgag gactggaaat taccgccgct
      781 ggcaggcaca accttttact gattgggccg ccgggaacag gtaaaacaat gctcgccagc
      841 cgtattaatg gcctgttgcc agatttaagc aatgaagagg cactggagag cgctgcgata
      901 ttaagtctgg taaatgctga atcagtacaa aaacaatggc ggcagcgccc gttccgctca
      961 cctcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg gtgcaattcc agggccaggt
     1021 gaaatttcgt tggcgcataa cggcg
//
LOCUS       DAZOZD010000544         1044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZOZD010000544 DAZOZD010000000
VERSION     DAZOZD010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.552"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..733
                     /gene="phoN2"
                     /locus_tag="ACHUPB_004774"
     CDS             <1..733
                     /gene="phoN2"
                     /locus_tag="ACHUPB_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR0761329.1"
                     /translation="KNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPPPPA
                     EDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTPILY
                     QLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHASFG
                     WAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLHNTP
                     EFTKSLSEAKKEFEELNTPTNELTP"
     gene            806..928
                     /locus_tag="ACHUPB_004775"
     CDS             806..928
                     /locus_tag="ACHUPB_004775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761330.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
BASE COUNT          305 a          231 c          232 g          276 t
ORIGIN      
        1 caaaaacttt cttctttttt gtattgctac aaatatgatt tttatcccct cagcaaatgc
       61 tctgaaggca gaaggttttc tcactcaaca aacttcacca gacagtttgt caatacttcc
      121 gccgcctccg gcagaggatt cagtagtatt tcaggctgac aaagctcatt atgaattcgg
      181 ccgctcgctc cgggatgcta atcgtgtacg tctcgctagc gaagatgcat actacgagaa
      241 ttttggtctt gcattttcag atgcttatgg catggatatt tcaagggaaa ataccccaat
      301 cttatatcag ttgttaacac aagtactaca ggatagccat gattacgccg tgcgtaacgc
      361 caaagaatat tataaaagag ttcgtccatt cgttatttat aaagacgcaa cctgtacacc
      421 tgataaagat gagaacatgg ctatcactgg ctcttatccc tctggtcatg catcctttgg
      481 ttgggcagta gcactgatac ttgcggagat taatcctcaa cgtaaagcgg aaatacttcg
      541 acgtggatat gagtttggag aaagtcgggt catctgcggt gcgcattggc aaagcgatgt
      601 agaggctggg cgtttaatgg gagcatcggt tgttgcagta cttcataata cacctgaatt
      661 taccaaaagc cttagcgaag ccaaaaaaga gtttgaagaa ttaaatactc ctaccaatga
      721 actgacccca taaagctgga cagcctgtat caggctatgg agggcccata gacaaatcta
      781 ccctatatga gcataggagg agtctatggg cacaccacgt tttacccctg aatttaaggg
      841 attactggaa aggctgggac atatccctcc ggcagaagca gaaaaagctt attatgctgc
      901 catcggaaac gatgatctgg caacctgagt tcacagataa aacattctct aggaaactcg
      961 gggcggttcc gttcaccaca tgcaatgtgg tgttgcaggg gaacggtctg caccatcccc
     1021 tatgtcgatc aatataacag aaat
//
LOCUS       DAZOZD010000545         1042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZOZD010000545 DAZOZD010000000
VERSION     DAZOZD010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.553"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(91..1042)
                     /locus_tag="ACHUPB_004776"
                     /pseudo
     CDS             complement(91..1042)
                     /locus_tag="ACHUPB_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          223 a          255 c          209 g          355 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa tc
//
LOCUS       DAZOZD010000546         1034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZOZD010000546 DAZOZD010000000
VERSION     DAZOZD010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.554"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            324..794
                     /gene="argR"
                     /locus_tag="ACHUPB_004777"
     CDS             324..794
                     /gene="argR"
                     /locus_tag="ACHUPB_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0761331.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          307 a          233 c          197 g          297 t
ORIGIN      
        1 aagtttagcg tttatgcatt taattgccgt aatcaggaac ctaacttacg taattaacag
       61 tcgcttatgc attagcgcaa cattccaaca ggtggtgaca atatacccta ccgttcagcc
      121 aaaacaacat caatttgata acaattaatt tacttttaaa cagaatttgc atgccgtgac
      181 gcaggcatgt ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc
      241 agccttcttc agggctgact gtttgcataa aaattcatct gtatgcacaa taatgttgta
      301 tcaaccacca tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag
      361 catttaaagc attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt
      421 tgcaggagca aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt
      481 ttggtgctgt acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac
      541 tgggtgtacc aaccacctcc agtccattga agaatctggt gctggatatc gactacaacg
      601 atgcagttgt cgtgattcat accagccctg gcgcggcgca gttaattgct cgcctgctgg
      661 actcactggg caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta
      721 ccacccctgc taacggtttc accgtcaaag acctgtacga agcgatttta gagctgttcg
      781 accaggagct ttaatctctg ccccgtcgct tctggcggcg gggaaaatgt tgcttattcc
      841 tctcaacccc ctgctttccc ctgcgattaa tttaacgaat agtgcgtttt accgcgacat
      901 atcattcaca caatgaatac atacggtaaa aaaagcacat tatgtaaaat tcattatcta
      961 attgaaaata ctagaattaa caataaataa ccgtgttttt aattcttttt tgttattaaa
     1021 attcacattt ttaa
//
LOCUS       DAZOZD010000547         1027 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZOZD010000547 DAZOZD010000000
VERSION     DAZOZD010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.555"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            300..410
                     /locus_tag="ACHUPB_004778"
                     /pseudo
     CDS             300..410
                     /locus_tag="ACHUPB_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            488..802
                     /locus_tag="ACHUPB_004779"
     CDS             488..802
                     /locus_tag="ACHUPB_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761332.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
BASE COUNT          310 a          234 c          222 g          261 t
ORIGIN      
        1 tgcacttaga aggatctctc cccgaattgt gtgatctgct taatttctaa aaagaaacaa
       61 acagttaact agactgagcg atactaccca tagaaccagt ttcccatgac gaacgacact
      121 ggacgcaaaa acaatcataa aaaacacaat aaatgtaatg aacacatccc accctattaa
      181 gtaaagtgca ctgtgataga ggaagggaaa tacaggaagg tgtggagcct cgcagatttc
      241 tggcatcata gtgcccaata cggaatgcat aaagccccct cagactggag actccacaaa
      301 tgaaatatac agtgtttaat tatatcaagt gcgattacaa tcgctgacga cggcacagtt
      361 ggtgtggagg tctcagtcct gaacaatttg aaaaccagaa cctcacttaa gcctgtgttc
      421 atattacgtg ggtaggatca tacttttata gaagctgtca gcggctacgt cccagccaaa
      481 tcacttcatg ctccagcgcc agagaggcaa ggttgggttt gctcagctgc cggttgattt
      541 tgttgcgatt gaaccggaca gcgtccaggg cgtcggcaaa agagcgaatc tcaccaatcg
      601 ttgtttcatt atccgcatta acgatagctt caagaaacgc caaggtttta tcgaattcat
      661 cagcaacagt ggctccgggc ataccgtgac cgtttataca aaacggcgtt ttcagcgtgg
      721 agttttcatg aatagcctca acacgaatgt cgtgaagcca gtgatgtatc ggctacgatc
      781 ctcagctgat gccagaccat aggactgaca ctacgaacag caattttaat gacgtaaatc
      841 ttcataacta caactaacag ggcatcatta tgaatgatgc cggttatttt accgctgttg
      901 gtcccttcat ttagggactg ccccaagttt tttcatactc tctttctcag agagtttagt
      961 ctctgggatt ccagacggtc catcattgag ccaagggagt aaagaactca aagcatttac
     1021 agaattg
//
LOCUS       DAZOZD010000548         1026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZOZD010000548 DAZOZD010000000
VERSION     DAZOZD010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.556"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..398
                     /gene="ipgB2"
                     /locus_tag="ACHUPB_004780"
     CDS             <1..398
                     /gene="ipgB2"
                     /locus_tag="ACHUPB_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR0761333.1"
                     /translation="NITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQ
                     VEKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQ
                     FFEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(660..782)
                     /locus_tag="ACHUPB_004781"
                     /pseudo
     CDS             complement(660..782)
                     /locus_tag="ACHUPB_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          331 a          214 c          211 g          270 t
ORIGIN      
        1 aaaatattac agattggaaa aatgatgaaa aaaaagtcta cgtatcccgg gtggtaaacc
       61 aatgtattga taagttttgt gcagaacaca gccgagaaat cggcgataac ctccgtaaac
      121 agatatttaa acaggttgag aaagattatc gcatttctct agacatcaat gctgcacaaa
      181 gttccataaa tcatcttgta tcaggaagca gttatttcaa aaagaaaatg gacgaacttt
      241 gtgaagggat gaatagaagc gttaaaaatg atacaacaag caatgttgca aatcttattt
      301 ccgatcagtt ttttgagaaa aatgtgcaat atattgatct aaagaaatta cgtgggaata
      361 tgagtgacta tattacaaat ttagaatcgc ctttctgatg acagaacatc ccgagtaaag
      421 aaactaagaa aagtaaatac ccaggaattg cctgagcggt gctatatttc tcctcgcaag
      481 taagtcctaa tatattgaat tgttgtatag ccagtttttg tattttccta gctcatggat
      541 gtgggatccc tctccccttt ctctgctcaa aggtaagcgg ctcgtcagaa tcatattgat
      601 atttactgag aagtcagatc aactttccaa ggcaacagat cgcgtacccg gtttgccggt
      661 cagtcctgga tatgttcaat aacgtaacgc agccattttt ctggctccac attgttcaga
      721 cggcgtgtgc cgatcagcga gaacaacacc gctgcatgtt cgtcaccgct gtcggaacct
      781 gcgagtcgag tcagccggag agagtttcac tctccagctg ctcacggaac cgtacgtgac
      841 actctcgcgt catacggctc ttgatcactc attcgatgca cgtagtaaat accagtgagc
      901 aaattgatct tacccagcaa tagtggacac gcggctaagt gagtaaactc tcagtcagag
      961 gtgactcaca tgacaaaaac agtatcaacc agcaaaaaaa cacgtaaaca gcattcgcct
     1021 gaattt
//
LOCUS       DAZOZD010000549         1026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZOZD010000549 DAZOZD010000000
VERSION     DAZOZD010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.557"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>1026)
                     /locus_tag="ACHUPB_004782"
     CDS             complement(<1..>1026)
                     /locus_tag="ACHUPB_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR0761334.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDR"
BASE COUNT          172 a          381 c          283 g          190 t
ORIGIN      
        1 acggtcatat ttccggctgc tgcgtaaacc atccggatat gtcactgacg tcagttgcaa
       61 ctggttgttg taaccatacc gtaccgtcct gccgtccggc agtaccactg aagtcagctt
      121 tccagacgcc atatgcagcc ggtactccgt ccgccgcccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cggctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgaacctg
      481 cgtcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaaaac
      661 ctgagaagag gaaacagaac ggggactggc cggggaagat aaggaagagg cgcgctgttt
      721 acgggacgct tccgcccgct gcgcctgcgt gctcagcgcc atgtgaaaac ggcggcccgc
      781 gccgtccgtc acccccgtca ccgcgcccgc cacgtcaccc tccgccgccc ggtggaaagt
      841 cagtgagcgc ccgaagccat ccaccacgac cgtcagtacc cggtatgcgg gcggctccgg
      901 ccagctgagt atccaccacg gcccctgaag gctgtttgtc gccaggtaca catgcggact
      961 caggcgaaca tcttccggca gcacctgcca gagcgcgctt agcggctgcg aactgtgctg
     1021 cgccgc
//
LOCUS       DAZOZD010000550         1024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZOZD010000550 DAZOZD010000000
VERSION     DAZOZD010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.558"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..594
                     /locus_tag="ACHUPB_004783"
     CDS             <1..594
                     /locus_tag="ACHUPB_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0761335.1"
                     /translation="IYPAENKEVMVQLMNQGNRSSLLQAWIDDGDTSLPPEKIQVPFM
                     LTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPPNSPEQEGKNALKFAMQ
                     NRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNVKVNYET
                     IMIAPLESQSVNVKSNNANNWHLTIIDDHGNYISDKI"
     gene            610..>1024
                     /locus_tag="ACHUPB_004784"
     CDS             610..>1024
                     /locus_tag="ACHUPB_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0761336.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          335 a          183 c          235 g          271 t
ORIGIN      
        1 atttacccgg cagaaaataa agaagtgatg gtgcagttga tgaaccaggg aaaccgttct
       61 tcgctgctgc aggcgtggat tgatgatggc gatacgtcat taccaccaga aaaaattcag
      121 gttcctttca tgttaacgcc accagtggca aaaatagggg caaattccgg gcagcaagta
      181 aaaatcaaaa ttatgccgaa taaactgccc actaataaag aaagcatttt ttatctgaat
      241 gttctggata ttccaccaaa tagtccagag caagaaggta agaatgcact gaagtttgcg
      301 atgcaaaaca gaattaagtt gttttaccga ccagcgggta ttgctccggt aaataaagcg
      361 acatttaaaa aattgctggt aaatcgcagt ggcaatggtt tggtgataaa aaatgactca
      421 gctaattggg tgacgatttc ggatgtcaaa gctaataatg tcaaagtcaa ttatgaaact
      481 attatgattg cccccttaga aagtcagagt gttaatgtca aaagtaataa tgcaaataac
      541 tggcatctga ccattatcga tgaccatggc aactatatta gtgacaaaat ttaattgcag
      601 gagctgccta tgttgagaat gaccccactt gcatcagcaa tcgtagcgtt attgctcggc
      661 attgaagctt atgcagctga agaaaccttt gatacccatt ttatgatagg tggaatgaaa
      721 gaccagcagg ttgcaaatat tcgtcttgat gataatcaac ccttaccggg gcagtatgac
      781 atcgatattt atgtcaataa gcaatggcgc gggaaatatg agattattgt taaagacaac
      841 ccgcaagaaa catgtttatc aagagaagtt atcaagcggt taggcattaa tagcgataac
      901 ttcgccagcg gtaagcaatg tttaacattt gagcaacttg ttcagggtgg gagctatacc
      961 tgggatatcg gggtttttcg tctcgatttc agtgtcccgc aggcctgggt ggaagaactg
     1021 gaaa
//
LOCUS       DAZOZD010000551         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZOZD010000551 DAZOZD010000000
VERSION     DAZOZD010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.559"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUPB_004785"
     CDS             complement(77..865)
                     /locus_tag="ACHUPB_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0761337.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUPB_004786"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUPB_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0761338.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZOZD010000552         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZOZD010000552 DAZOZD010000000
VERSION     DAZOZD010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.560"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..324)
                     /locus_tag="ACHUPB_004787"
     CDS             complement(<1..324)
                     /locus_tag="ACHUPB_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761339.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDR"
     gene            complement(321..>1023)
                     /locus_tag="ACHUPB_004788"
     CDS             complement(321..>1023)
                     /locus_tag="ACHUPB_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFR0761340.1"
                     /translation="ALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAFFRCLNG
                     SRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAGHRLFPS
                     VPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPATVGSWL
                     SRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRGAPVQVR
                     ALEQWMIPNKLENVI"
BASE COUNT          236 a          309 c          263 g          215 t
ORIGIN      
        1 tctgtcacgg gctgccattc gctcgcccga cagcgccttg ctgatatggc tgcgcaccag
       61 attatccagg agtgcctgcg cttcttccgg tgaaaagcca cctggtatct ctgtgcgcaa
      121 cagccctttc ctgtacagaa gctgcagcag atcaacctgc gaccgcacag caactatcag
      181 tacataacgg acaacattcg cactggcagt ggtttctgta tcattcagca gttcttccgg
      241 atcgttcccc ccggtgttgc agagcaacgg caacagaggt cgcatccacg cgggaacctg
      301 gtatttttcg tctgatttca tcatattacg ttctccagct tgttcgggat catccactgc
      361 tcaagtgcac gtacctgtac cggtgcacca cgaccggcct caccggcttc aaaaatcagc
      421 cgccagagtg gttcctcctg ccagaaaaac cggtcaaatg atgacagtga tggaaaacgc
      481 ccgcaccacc cccagaaaat cgtttccaga tcatgctcct caacaacacc agaccagcgg
      541 gacaaccagc ttccgaccgt ggcgggagta tgaaacgcaa ggtcaatctg cgcctgagca
      601 aaacatgtct ggtattccag ttcccgtcgt tctgcctcac gttcccgctg gcggctgtat
      661 ttctgctctg tcagcgtggt tttctgacga cgacgctcac cttcacgaaa aggcacggac
      721 gggaacagac ggtgccccgc atcggatggc aggggaagaa cacagacttc cccaaaactt
      781 tcaatcagtt tatccacggc agccagccac aggagacggt tgccatgaaa ttcctccttc
      841 gtcagcgcag gggcaaaaaa gcggagatcg gtcagcgaaa tccggcgact gccattcagg
      901 cagcgaaaga aagcggtggc ataaggccat gctgaaagtg tgcgtccctg acgacgacgt
      961 tgttctgtga cagccaccgc ctgcatggcg gcctgttgtt ctgcgctgag ggggatcagt
     1021 gcc
//
LOCUS       DAZOZD010000553         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZOZD010000553 DAZOZD010000000
VERSION     DAZOZD010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.561"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            393..817
                     /gene="yqeK"
                     /locus_tag="ACHUPB_004789"
                     /pseudo
     CDS             393..817
                     /gene="yqeK"
                     /locus_tag="ACHUPB_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            853..933
                     /locus_tag="ACHUPB_004790"
     CDS             853..933
                     /locus_tag="ACHUPB_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761341.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          328 a          154 c          168 g          359 t
ORIGIN      
        1 ttcgcagctt ctgataatgt caaatatcac cttttctcat gctttattct atcaaaaatc
       61 gctcattttt acttttttta ccataatgtt actcagtagc atcggcaatt tacataactt
      121 acacgttgtt ttttatatct cgaaaaaaca gatagatagt gtgcgcactc gaaatgatat
      181 tggtaacata aatagcttta ttcgtgataa acatctcaaa agatttcctt tattatattc
      241 gaaattcatt tgatcaaggt tatcacctta tggagacgtt acgtagaatg actatatttg
      301 ttatcggcac attaggggta ttaattttag tattgatgga aggtgattat tgaacagtaa
      361 catcgagtca cggtaaaaat gtgaggtata caatggacat tgagttttcg cagattcatg
      421 aaatggtcta tatgcatgat attgtaaatt cggactcgaa aaagaaacca aggattccgt
      481 taaaaaaatt ccttaatgct gagaatgttc tcacgcaaac aacatcatgg acattaaatt
      541 caagatatgt gaatgttaat tctgtcaata aagtcaacgt aaagagtaaa gttaaaaaca
      601 gttatatctc acgatctgtc aatgatgagt ttagtttaac agatgatgaa atcaattctt
      661 ttaaagaaac gttagtcctt tcctctattg attcctgagt aaactggttc ttaataatcc
      721 tctttctgta ttgtttacat cgactgtacg aaggaataat aatcgagcta agatgaatgt
      781 cgagttcgac tcatggattt gcaccagatg ttgttgatac atgatatttt acattgcaag
      841 gagatgtcta aaatgaaaga tgttgatcaa aactttgatg ctttagactg ccatatactg
      901 cgagaatatt taattttatt gttttatgat taacgaggtt ccatcttttt tgttatctat
      961 tttagtttac cttgcacaat gcattattgc tctgttacca atgctcaaa
//
LOCUS       DAZOZD010000554         1005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZOZD010000554 DAZOZD010000000
VERSION     DAZOZD010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.562"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..955)
                     /locus_tag="ACHUPB_004791"
     CDS             complement(<1..955)
                     /locus_tag="ACHUPB_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR0761342.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAF"
BASE COUNT          304 a          209 c          212 g          280 t
ORIGIN      
        1 taaatgcctg gcgcgatttc atcagtccgc ccagattgat ctgcccgcta ataaactgcg
       61 gcaataacag aaagtaggga agaacgctaa gcgagcgcga ataaatattt tgccagtaat
      121 caagccaccg ctgacgattc attaaacgat gccagttctc tttaatggta tgaaaattgt
      181 cgcgcaactc ctggcgttgt agagactcag cattgcttag cgcaatcagt tctgtctgct
      241 tattatgctg cacaagatta gttcgaaatg tcgcttcgct acgttgtttt tccacattaa
      301 gcggacgaat acgtttaccc accttatggg taaataaagt tccaccgatc acaatgagca
      361 cgacggtata gaccatatac ccctggatat tccattctgt tccaccaaca gtgaatgaga
      421 gcgtacccgc gctttgccaa agaataacgg taaaggtgat cagcatgcta agtgactgga
      481 tgaagccaaa tgaaagactg agcgttttgc tgatcagtaa gagaatgtct tcagcgatac
      541 gttggtcagg gttatctgta tttttatgct cgccgtagat ctgcgtgaag tagtagtttt
      601 tatctgcaaa ccagcgatta aggtagtaat ctgttaacca ttcgcgccag cgaatagtca
      661 gtagtttaat taaccatgtt ttgtttacgg atattaatac aaagatcccc agcagggcag
      721 ggaaccaaag aacaagctgc caaagcttat cggtttcttt ctggcttagc gcattgaaaa
      781 aatcattatt ccagtcgttt aaccagacct gaattttaac cacgccaagg atcattgcga
      841 ggataataat gattaacaac actgaagttt tattattctt acgcagccaa aagggcttca
      901 gcagacagag atactttgct atcaacatat aaaacgtaat gggaatggtt atcattagcg
      961 aaaattgatt gtgccatcca atgattatct atggcaatga tttac
//
LOCUS       DAZOZD010000555         1000 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZOZD010000555 DAZOZD010000000
VERSION     DAZOZD010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.563"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>1000
                     /locus_tag="ACHUPB_004792"
     CDS             <1..>1000
                     /locus_tag="ACHUPB_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFR0761343.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          234 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattggt
//
LOCUS       DAZOZD010000556          996 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZOZD010000556 DAZOZD010000000
VERSION     DAZOZD010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.564"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..>996
                     /gene="umuC"
                     /locus_tag="ACHUPB_004793"
     CDS             1..>996
                     /gene="umuC"
                     /locus_tag="ACHUPB_004793"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFR0761344.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALN"
BASE COUNT          259 a          242 c          279 g          216 t
ORIGIN      
        1 atgtttgcac tggctgatat caacagtttc tacgcctcat gtgaaaaagt tttccgcccg
       61 gaccttcgca acgaaccggt catcgtactc agcaataacg atggctgtgt gatcgcgcgc
      121 agcccggagg caaaagccct tggcatcaga atggggcagc cctggtttca ggtgagacaa
      181 atgcgcctgg agaagaaaat acatgtattt tccagcaatt atgcgctgta ccacagcatg
      241 agccaacggg ttatggctgt tctggagtcg ctttctcccg cagttgagcc ctactcaatt
      301 gatgaaatgt tcattgattt gcgggggata aatcattgca tctctccgga gatttttggt
      361 catcagctca gggaacaggt aaagagctgg acaggactca ccatgggggt gggcattgcg
      421 cctacaaaaa cgctggctaa aagtgcccag tgggcaacaa agcaatggcc acagttttcc
      481 ggtgtggtcg cgctgacggc agaaaaccgt aatcggatct tgaagctact ggggttgcag
      541 ccagttggtg aggtctgggg agtaggacgc agactgacgg aaaagctgaa tgcgctgggt
      601 attaacacag cactgcagct ggcgcaggct aacacggcat tcatccggaa aaacttcagc
      661 gtcattcttg agcgtacggt acgcgaactc aacggcgagt cctgcatatc tctggaagaa
      721 gcaccgccgg caaaacagca gattgtctgt agtcgcagtt ttggtgaacg aatcacagac
      781 aaagatgcca tgcaccaggc tgttgttcag tatgcagagc gggccgcaga gaaactacgt
      841 ggggagcgtc agtattgccg gcaggtgacg acatttgtac ggacatcccc ctttgcagta
      901 aaagaaccct gttacagcaa tgccgctgtg gaaaagcttc cattgcccac acaggacagc
      961 cgggacatta ttgccgcagc atgcagagcg ttaaac
//
LOCUS       DAZOZD010000557          992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZOZD010000557 DAZOZD010000000
VERSION     DAZOZD010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.565"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..616
                     /gene="ospI"
                     /locus_tag="ACHUPB_004794"
     CDS             <1..616
                     /gene="ospI"
                     /locus_tag="ACHUPB_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR0761345.1"
                     /translation="GTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSELAATLQKM
                     INPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQIDSEGIVSH
                     PNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLDVQTGKGFN
                     LYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            731..992
                     /locus_tag="ACHUPB_004795"
                     /pseudo
     CDS             731..992
                     /locus_tag="ACHUPB_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          307 a          155 c          246 g          284 t
ORIGIN      
        1 gggaactgct ggatatgagg cacatacaga ggagggaaat gtaaatgtta aaaaattgct
       61 ggaatcttta aactctaaaa gtcttggtga tatggataaa gattctgaat tggcagcgac
      121 tttacagaaa atgataaatc cgtctggtgg ggatggtaac tgttcagggt gtgcgcttca
      181 tgcttgtatg gctatgttag ggtatggagt aagagaggct cccgtaccta atgaaatatc
      241 agagtatatg accggatttt ttcataggca tttagagcaa attgatagtg aaggcatagt
      301 gtcacatcct aatgaaactt atagtaaatt tcgtgagcgt attgcagaaa atattttaca
      361 aaacacttca aaaggaagtg tagtaatgat ttctattgaa caggccactc attggatagc
      421 tggatttaac gatggtgaaa aaattatgtt tttggatgtt caaacaggaa agggatttaa
      481 tctttatgat cctgtagaaa aatcgccaga tgcatttgta gatgaaaata gttcggttca
      541 ggtaattcat gtatctgatc aggaatttga tcactatgct aacagtagta gttggaagag
      601 taagaggctt tgctgatgct gagagcctgt acatagattt gagccggccc cttgaaacgc
      661 cagacagtgg atgtatctta aaataagaga taggcttgga tatatgtcta acactagttc
      721 taattttgag atgactggaa ctttgctggg gctggagctt cgtaaacgta aaactcctca
      781 ggaaaaaatt gccattattc agcagacaat ggagcctggc atgacggtgt cccatgtggc
      841 gcgtttgcat ggtattcagc ccagcctgtt gcttaagtgg aaaaaataat atctggaagg
      901 aagtctcact gctgttgctg ccggagagga tgtcgttcct gcctcagaac tggctgccgc
      961 tataaaacaa ataaaagagt tacagcgctt gc
//
LOCUS       DAZOZD010000558          990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZOZD010000558 DAZOZD010000000
VERSION     DAZOZD010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.566"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            43..303
                     /locus_tag="ACHUPB_004796"
     CDS             43..303
                     /locus_tag="ACHUPB_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761346.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            300..890
                     /locus_tag="ACHUPB_004797"
     CDS             300..890
                     /locus_tag="ACHUPB_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761347.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
BASE COUNT          224 a          270 c          313 g          183 t
ORIGIN      
        1 tgatttttac ctgtcagaac attatcagat ggaacgtgca ggatggatca ggaaatgacg
       61 ttttcactca gctatgaaca actgacccgg tttgcggaaa aacgcatccg ggaatgcaat
      121 ctggacagcc acggcgtaac ttatctctgc gagtcagcca aagccggagc cgtgctgatc
      181 ttctggcatg aactggccat aaatggctat acctcaatga atgccataaa gagacaggag
      241 atcattgacg ccgaccatca gcgcctcaga aagctcatct ggccggagga tgactggaaa
      301 tgactcagag ccgccgaccg tcgccactgc agcggagagt gctgattgtg ctggctgccc
      361 ttgatgaaaa acggccagga cccgtgctga cgcgggacat agaacgggtg ctggagcaaa
      421 gcggggaagc cccggtctac gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg
      481 ccggctggct gcgtaccctg cgcgctccga acctgcagct ggcagtggag ctgacggacg
      541 ccggccgggc cgtggcacag cctctgctgc tggcagaaca ggaccgcatc cgggcagaac
      601 agcgtgcggc agaagtcgtc gtactgccgc tggtcccggc agcaggtctg cccgcaggcg
      661 gcacttctgc cactgatctg gcggtggagc tgaacggcat aacgtatcag gcctgccgtg
      721 gggattttgt ggtgcgtctc gacggcagca cctgtctgca gttatggaat aaagaaggct
      781 gggtggtacg ccgggagggc gatccgctgg aagtggcgca gtggctgccg gcctgtcacg
      841 atgcaggaat ggaggttcgc gtacagataa atgaaagtgc cgctccgtaa gaacacgcct
      901 gtgcacggtt tacacatcat cccatggtgg cagtcggtta actttcctgc gccaccagtg
      961 gaaagaggcc atgagcagtc cggacaaaaa
//
LOCUS       DAZOZD010000559          989 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZOZD010000559 DAZOZD010000000
VERSION     DAZOZD010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.567"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(360..497)
                     /locus_tag="ACHUPB_004798"
                     /pseudo
     CDS             complement(360..497)
                     /locus_tag="ACHUPB_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(576..746)
                     /locus_tag="ACHUPB_004799"
     CDS             complement(576..746)
                     /locus_tag="ACHUPB_004799"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0761348.1"
                     /translation="MTHHLGCEKNQLRSGSNSRNGCLTKIITTGDEPLEIRTLRDRNG
                     TFEPQQLKKNQP"
BASE COUNT          286 a          216 c          179 g          308 t
ORIGIN      
        1 ctttttgcta aaaaaaacaa taacctattt ttgtaatgta atagatatta gtctattgcc
       61 aatctaaaaa aaccattgcc acattgaatg gctatattca ccaataaatt ctttcgattt
      121 cgtatattga catatcgtag atatatctta tccctaagat tcaataatcg agtccgttta
      181 ccattctgtg caagcgcctt cactcagaag tgaccgtcaa ggcgatcacc aaacttgata
      241 ataaagcagc tcattgccat acgccagtcc ttaaggggcg ttgtccattt ctgtgatgct
      301 gcctagattt aactgccagc tccggcttat ctacatctaa atatgtgagc ctaataccgc
      361 tacactttgg ccagactatg tttttctccg ggaagtgggc taaccgtctc cataaagtga
      421 cgaacttttt tcaacagtgg ccatattgaa cggcactgat gattacgggt tattgtctca
      481 tgaagagccg gccacatcac tgacagcagt gacttcaggc cttcagcatc agacagcctg
      541 aaccagttaa tccatcgccc gacagattta gtaatctagg gctggttttt cttcagctgt
      601 tgcggttcga aagtgccatt acgatcacgc agagtacgga tttccagagg ttcatctcca
      661 gtagtgataa tcttcgttaa gcaaccattg cgggaattgc tgccagatct aagctgattt
      721 ttctcgcacc caagatgatg agtcatatta gcattgagta cggtttcatt ttcagcatcc
      781 aatcaaactg actgagatct tcaggatttt tgagactttt ggctagttca ttagctaaag
      841 cccgcaactt attagatgtc aactgttttt cattcataaa ttcaccttca tttgatatca
      901 aattaaatag ttcaacatca gagaattata caaacttatg tacaggctcc tatgatcttc
      961 gaatcctgaa gattatagtg aaaataagt
//
LOCUS       DAZOZD010000560          981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZOZD010000560 DAZOZD010000000
VERSION     DAZOZD010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.568"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            2..145
                     /locus_tag="ACHUPB_004800"
     CDS             2..145
                     /locus_tag="ACHUPB_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761349.1"
                     /translation="MHVKNIIPIITATLILNYLNNSERSLVKQILIEDEIIVCATYLI
                     PDI"
     gene            147..491
                     /locus_tag="ACHUPB_004801"
     CDS             147..491
                     /locus_tag="ACHUPB_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0761350.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            495..830
                     /locus_tag="ACHUPB_004802"
     CDS             495..830
                     /locus_tag="ACHUPB_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0761351.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          279 a          209 c          250 g          243 t
ORIGIN      
        1 cattcacgta aaaaacataa ttcctatcat cacagccacg ctcattttga attatctgaa
       61 taatagcgag cgttctttag ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc
      121 aacttactta ataccggaca tctaagttga agaaaaaaat acttgccttc gggcttatct
      181 cagcattgtt ctgctccacg ccagcgatgg cggatatgaa ccgcaccacg aaaggtgcat
      241 tgctaggcgc aggcgttgga ttacttactg gcaatggtgt taatggcgta ctgaaaggtg
      301 cagctgttgg cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcac
      361 gtaaaggtgc aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg
      421 gcctcgaagg ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta
      481 aaatgaaata aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg
      541 caccaatgac ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata
      601 ttacttgcgc agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg
      661 ttgcggtgat gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg
      721 aactgagcgc caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc
      781 tgttgatcac ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttaatat
      841 gttaccgcgt cgatgaattc gacgcggtat tttagctaac ggggatcaga acttatattt
      901 caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt
      961 accccagaca ctggccgccg g
//
LOCUS       DAZOZD010000561          980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZOZD010000561 DAZOZD010000000
VERSION     DAZOZD010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.569"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..244)
                     /locus_tag="ACHUPB_004803"
     CDS             complement(<1..244)
                     /locus_tag="ACHUPB_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0761352.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEI"
     gene            complement(845..>980)
                     /locus_tag="ACHUPB_004804"
     CDS             complement(845..>980)
                     /locus_tag="ACHUPB_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0761353.1"
                     /translation="EVIVKATSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH
                     "
BASE COUNT          238 a          173 c          238 g          331 t
ORIGIN      
        1 taatttccgg ggggatttca ggtaatgttg tcaggcctaa ttcagacaag tccaggttcg
       61 tctcttggtt ttgtagacat gatactagtc gctgaaaagc gatgtctcgt tgttcttctt
      121 gtatgcggtt atttttccat tcagtccaat gggttagata attttcatat gcacggctag
      181 tgtcgattga ataagtggag aacgaatttg aaattaatct gtgattgtta tttgtcggga
      241 gcataaatat cagggcgtat tctttatgtt gagaggtgtt ggattctttt ttatctgctt
      301 tgcaatgtta ttggttccct tcagcagtag acaggaactt ctggaggagg cgaatatata
      361 gtttgtgtaa gcatattaac tccatatgtt atatattgaa gaacttctgc tttacactat
      421 cctaaactgg atcggtttaa tgtaaaaaaa cgatactttt tgggggaggg ggcaattttt
      481 ccttaaggta tgaaaaaagg agctgaagct atataatagc tattaatgcc actggtatca
      541 gtgaatcatg tatataaaaa actgtgttaa ggaggctgtc cattagcggt tttttattct
      601 tctggaaccg cggcatcttt gcgtatcttt ttgtcttctc accgtctggt cggtgttttg
      661 ctgagactgc taatttcctg tctttgctag tgctgtactg gtactgccca atcatgattg
      721 gcgggggcgg gcggagttga gaccgcagac acgtcgtatg caagaacgtg ctgcggctgg
      781 ctggtgaact tccgatagtg cgagtattga atgatttcca gccgttactg attttacgtg
      841 ctaattagtg aacaaaccac tcgtcagcag actcccaggt atctttcaga gtttcctgaa
      901 caaaagtttt agctgaatct ttatcggcgg tgcgcgtaac agaaaggcca tcgttgctgg
      961 tggcttttac gatcacctct
//
LOCUS       DAZOZD010000562          979 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZOZD010000562 DAZOZD010000000
VERSION     DAZOZD010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.570"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>979)
                     /locus_tag="ACHUPB_004805"
     CDS             complement(<1..>979)
                     /locus_tag="ACHUPB_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0761354.1"
                     /translation="GTQLNQSDNFLDSLREFFAILNSSRKGELSTWDTIYLHLILAIN
                     ADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDNEVEIKAGNERISI
                     GIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHTIGSKIANVIFEKL
                     YSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIALMNTFYDSDNTSG
                     EVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFP
                     EMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDAS"
BASE COUNT          306 a          195 c          149 g          329 t
ORIGIN      
        1 tacttgcatc ttcacgtacc atcgtggcaa ctggaacagg cagagacatg aaaacttctt
       61 cattcaccat caagtgaatc atgcattgcg ttccctttgt catatccaca catacgctac
      121 aaatatcgtg gtaatgcgaa gagcgcattt caggaaacag attaaacacg gtatcttgaa
      181 aggcactaaa tcgtgttaat aaatcctgat aatgaacagg ttttgtttca aaattatcat
      241 cagcaaatcc aagcactatt gtatgctcta catctaacgc agtttcaaat ggctcttctt
      301 ctgtaaaatc attatttaaa acctcacctg acgtattatc actgtcataa aaagtgttca
      361 tcaatgcgat attgtttgtt acagcgtctt tttcgatatt ctcactaatt gcatatcgcc
      421 tgctataatc ataaaaatga ataaacgcgc cctttataaa ggatatatat tgttctttgt
      481 taaagtcaat acggctgtat aatttttcaa aaatcacatt cgcaatttta ctaccaatgg
      541 tatgcctgta taataatggg tgcgattcaa tcgtaaaatg cttgtctaat tgatcttgta
      601 atttattctc atagtccaga gggaaaagaa actgttgtct ttcaagtttc ccattcgata
      661 cattacgtat tccaatagag attctttcat tacccgcctt tatttcaact tcgttatctt
      721 ctgatttacc cagatatttt ttaacatcag tctcgaaagc attactaaaa aaagtattaa
      781 attggtaatt cgctgatggc atattttccg cgagcaacac atcatttttt atgagatcac
      841 tgtcagcatt tattgctaat atcagatgca agtagatggt atcccatgta gacaactcac
      901 ccttacgact ggaatttaat atcgcgaaaa attccctcag tgaatccagg aaattatcac
      961 tttgatttag ttgtgtgcc
//
LOCUS       DAZOZD010000563          978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZOZD010000563 DAZOZD010000000
VERSION     DAZOZD010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.571"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            15..>978
                     /gene="istA"
                     /locus_tag="ACHUPB_004806"
     CDS             15..>978
                     /gene="istA"
                     /locus_tag="ACHUPB_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR0761355.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          234 a          258 c          260 g          226 t
ORIGIN      
        1 attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc
       61 agggaatgag tagccgggcg attgccagag aactggggct ctcccgcaat accgttaaac
      121 gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac
      181 tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa
      241 tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc ggaatgacca
      301 ttctcagggc attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt
      361 tcgaaactga acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct
      421 caccgcttca cgtgttcgtt gctgttcccg gatacagccg aatgttgtac atcgaattca
      481 ctgacaatat gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg
      541 gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg
      601 catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc aaggagatgg
      661 gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga
      721 tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg
      781 ggagcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg
      841 ctaaccaacg aaagcatgaa acaatccagg cccgtccctg tgatcgctgg ctcgaagagc
      901 agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat cctggtgaaa
      961 atctggtgag cttcgaca
//
LOCUS       DAZOZD010000564          978 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZOZD010000564 DAZOZD010000000
VERSION     DAZOZD010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.572"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            87..>978
                     /gene="tdcA"
                     /locus_tag="ACHUPB_004807"
     CDS             87..>978
                     /gene="tdcA"
                     /locus_tag="ACHUPB_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0761356.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEY"
BASE COUNT          268 a          188 c          224 g          298 t
ORIGIN      
        1 gttaatttgt gagtggtcgc acatatcctg ttcatttcat tttgatacac ttcatgccgt
       61 caatgaggta attaacgtag gtcgttatga gcactattct tcttccgaaa acgcagcacc
      121 tggtagtctt tcaggaagtc attagaagtg gctctatcgg ctcggctgca aaagaattag
      181 ggttaactca accggccgtc agtaaaatca ttaacgatat tgaagattat tttggtgtgg
      241 aattagtggt gcggaaaaat accggtgtaa cattaacacc tgccggtcaa ttgttactct
      301 cccgttccga atccattacc cgtgaaatga aaaatatggt taatgagata agcggtatgt
      361 cttctgaggc ggtggtggaa gtctcatttg gttttccttc attgattggt tttactttta
      421 tgtcagggat gatcaacaag ttcaaagagg tgttcccgaa agcgcaggtt tctatgtatg
      481 aagcgcaact gtcttcgttc ttaccggcaa tccgcgacgg tcgactggat tttgcgattg
      541 gtacattaag tgcagaaatg aagcttcagg atttacatgt tgagccgctg ttcgagtccg
      601 agtttgtgct ggtagccagt aagtcccgaa catgcaccgg caccaccacg ctggagtcgt
      661 tgaagaacga acagtgggtg ttgccacaaa ctaatatggg gtactacagc gaactgctta
      721 ctacgttaca aagaaatggc atcagtattg aaaacatcgt taaaaccgac tcagtcgtga
      781 caatttataa tcttgttctc aatgctgatt tcttaactgt aattccttgt gatatgacgt
      841 caccttttgg ttctaatcaa tttattacta ttccggttga agaaacatta cctgtggcac
      901 aatatgccgc ggtatggtcg aaaaattatc gtattaaaaa agcagcatcg gttttggtgg
      961 aattagccaa agagtatt
//
LOCUS       DAZOZD010000565          973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZOZD010000565 DAZOZD010000000
VERSION     DAZOZD010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.573"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>973
                     /gene="gspA"
                     /locus_tag="ACHUPB_004808"
     CDS             <1..>973
                     /gene="gspA"
                     /locus_tag="ACHUPB_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0761357.1"
                     /translation="REVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKHRRVI
                     TLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLIIIEN
                     AHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEHELKA
                     SILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTAKQW
                     RMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPVVKQD
                     MTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          243 a          226 c          264 g          240 t
ORIGIN      
        1 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
       61 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      121 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      181 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcctggacg cgagatgaga
      241 tggcggggca attactgcat gcgcttcatc gaactgatag tcgctgccct cttattatta
      301 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      361 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt
      421 ttaaaaaaca ggacattgaa cttgtctcga caggacgtct gacggagcac gaacttaagg
      481 caagcatcct tgaaggtcag aatattgacc aacccgatct ccttttaacc tccagagttc
      541 tgaaacggat agctttatta tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa
      601 caattcgctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      661 tgatttaccg catattgggt gataaccgcc cccggaaaat gcagcttgcc gtggtaatga
      721 gtggaaccat tatcgctatt acctgtggtt ggctactgct gtcctctttc acagctccgt
      781 tccccgttcc tgcgtggctg gtacccgtaa cgccagtagt taaacaagat atgactaagg
      841 atattgctca tgtggtgatg cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg
      901 ggtacgaggt cccagctgat tcggcctggt gcgatcaggc agtaagagcg gggctggcat
      961 gtaaaagtgg gaa
//
LOCUS       DAZOZD010000566          966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZOZD010000566 DAZOZD010000000
VERSION     DAZOZD010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.574"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            70..489
                     /locus_tag="ACHUPB_004809"
     CDS             70..489
                     /locus_tag="ACHUPB_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0761358.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            489..>966
                     /gene="umuC"
                     /locus_tag="ACHUPB_004810"
     CDS             489..>966
                     /gene="umuC"
                     /locus_tag="ACHUPB_004810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0761359.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          234 a          207 c          255 g          270 t
ORIGIN      
        1 atcagtattg atctgctggc aagaacagat tactgtatat aaaaacagta taacttcagg
       61 cagattatta tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta
      121 tttagcgatc ttgttcagtg tggctttcct tcaccggcag cagattacgt cgaacagcgc
      181 atcgatctga atcaactgtt gatccagcat cccagcgcga cttacttcgt caaagcaagt
      241 ggtgattcta tgattgatgg tggaattagt gacggtgatt tactgattgt cgatagcgct
      301 attaccgcca gccatggtga tattgtcatc gctgctgttg acggcgagtt tacggtgaaa
      361 aaattgcaac tacgcccgac ggtacagctt attcccatga acagtgcgta ctcgcccatt
      421 accatcagta gcgaagatac gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg
      481 atgcgctgat gtttgctctc tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt
      541 ttcgccctga tttatggggt aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta
      601 tcgcccgaaa cgctgaggca aaggcgctgg gtgttaaaat gggcgatccc tggttcaaac
      661 aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag cagcaattat gagctttacg
      721 cagacatgag caatcgggtg atgtcgacgc tggaagaact atcgccccgc gtcgagattt
      781 acagtattga tgaggcattc tgcgatctga caggtgtgcg taattgtcgc gatctgactg
      841 attttggcag agaaattcgc gcaacggtgc tacaacgtac ccatcttact gttggtatgg
      901 ggatcgccca gaccaaaacg ctggctaagc ttgccaatca tgcggcaaaa aaatggcagc
      961 ggcaga
//
LOCUS       DAZOZD010000567          966 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZOZD010000567 DAZOZD010000000
VERSION     DAZOZD010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.575"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>966)
                     /gene="mxiA"
                     /locus_tag="ACHUPB_004811"
                     /gene_synonym="virH"
     CDS             complement(<1..>966)
                     /gene="mxiA"
                     /locus_tag="ACHUPB_004811"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR0761360.1"
                     /translation="MGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAG
                     KIITTFGQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMS
                     IDADLKAGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGI
                     SVGMSQHGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNI
                     MSQIFGNPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSES
                     DSSGYTGTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINA"
BASE COUNT          352 a          212 c          141 g          261 t
ORIGIN      
        1 tgcatttatc ttattttcgg caaataataa aattaaagga acggtttctg aactaatagc
       61 atccagattt tctatcattg ccaaagatga gtcatggcta ttatcaatat caaatgtacc
      121 agtatagcca ctagaatcag actcagacag acttttttct ttttctacga cctttttata
      181 atagaataaa gccgtcaaag taactgctat caggaaaaaa acaaaaaacg gaaaacctgg
      241 caacatccct atcgccaagg caagagctga tgtaacaatg agaacaaaag gattcccaaa
      301 tatctgggac attatgttac gtcccatatt atcgctatca ccattaactc ttgtaacgat
      361 aaatcctgca cttatagaaa ttaacagagc aggaatctga gagaccaatc cgtcaccaat
      421 agttagtatg gtataagtag ataatgcacc agatagggac attccatgtt gactcatacc
      481 aacagaaata cctcctatta aattgacaaa aataataatg atgccagcaa ttgcatcacc
      541 tttaatgaac ttcattgctc catcaaatga accatacaat tgactctcac gttccagaat
      601 actgcgtctt tctttagctc ctgcggcatc aataattcca gcctttaaat ccgcatcaat
      661 gctcatttgt ttccccggca taccgtcaag agaaaagcga gcagctacct ccgcaaccct
      721 ttcagatcct tttgttataa caatgaactg cacaacagtt actattgaaa aaataacaaa
      781 acctacagcc agactatcac caatgacaaa ctggccaaat gtagtaataa ttttacccgc
      841 atccgcatca actaaaatta gtcgactagt acttattgat aaagcgagac gaaataatgt
      901 cgttatcaac aaaaccgaag ggaatgtaga aaaactcaat attctttcaa tataaaatga
      961 tcccat
//
LOCUS       DAZOZD010000568          959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZOZD010000568 DAZOZD010000000
VERSION     DAZOZD010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.576"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>959
                     /locus_tag="ACHUPB_004812"
     CDS             <1..>959
                     /locus_tag="ACHUPB_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HFR0761361.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          173 c          266 g          248 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggta
//
LOCUS       DAZOZD010000569          957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZOZD010000569 DAZOZD010000000
VERSION     DAZOZD010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.577"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..395
                     /locus_tag="ACHUPB_004813"
     CDS             <1..395
                     /locus_tag="ACHUPB_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0761362.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            569..>957
                     /locus_tag="ACHUPB_004814"
     CDS             569..>957
                     /locus_tag="ACHUPB_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0761363.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          327 a          177 c          188 g          265 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg
      601 taaagatggt atctattttc accttagaga taatgtggaa tcttttcttg ccttaacagc
      661 tactgaacag atggactatg actcaccgat gatggtgaca actcaggcta tgcaatcggg
      721 gcaaactatc actgataatg ttcagagagc acctaagacc atctctatta gcggtgttgt
      781 agtggttgat tcgacgggtt cagttctatt aaccagacag gggcaacttg tagaaacctt
      841 tatttctacg gttgaacaat ggcgagacca gaagcaaatc atcactattg tttgtagaga
      901 tggaatagct atcactgata gtatcattac tcagtttaaa gcgagtaaag agcatac
//
LOCUS       DAZOZD010000570          951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZOZD010000570 DAZOZD010000000
VERSION     DAZOZD010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.578"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(50..951)
                     /locus_tag="ACHUPB_004815"
                     /pseudo
     CDS             complement(50..951)
                     /locus_tag="ACHUPB_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          252 a          194 c          173 g          332 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat a
//
LOCUS       DAZOZD010000571          951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZOZD010000571 DAZOZD010000000
VERSION     DAZOZD010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.579"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHUPB_004816"
     CDS             <1..365
                     /locus_tag="ACHUPB_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0761364.1"
                     /translation="QEKLIWLRAQMKLTSLCWALKELAKDIWSRPWSEERRNDWQRWL
                     SLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYRNR
                     ERFKLGVMFHYGKLNMAF"
     gene            395..796
                     /locus_tag="ACHUPB_004817"
                     /pseudo
     CDS             395..796
                     /locus_tag="ACHUPB_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          274 a          200 c          268 g          209 t
ORIGIN      
        1 ggcaggaaaa gctgatatgg ctgcgtgcgc agatgaagct gacgagcctg tgctgggcgc
       61 tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga cgcaacgact
      121 ggcagagatg gctgtcgctg gcggccaaca gtgacgtccc catgatgaag aatgtggcta
      181 aaacgatagg gaaaaggttg tacggtatcc tgaacgccat gcgccatggc gtatcaaacg
      241 gaaatgcgga agcactgaac agcaagatca ggctgctgag aataaaagcc aagggctacc
      301 gcaaccggga gcgctttaag ctgggagtga tgttccacta tgggaaactg aatatggcgt
      361 tctaagcctt cccaccatga tcggggaaga cccagatatg aacaggcaca ttcagagtta
      421 ctttaaagag cttactgaac gtctgaggtg ttagagccat gactgccgca gcattgctgg
      481 cggaaatgcc tgaatcgagc agcctctcga gacgtgaaat cagcgcactt gtcggtgttg
      541 ctcaggtaaa ccgggactcg ggaactctgc gcggtcgacg aaccattttc ggtgattgtg
      601 ctggggaaga acagctctgt acatggcggc gcttgaggca ccccggttta atctggtgat
      661 aaaggcgttt tacatgcgcc tacttgcagc cggaaacgcc aaaaaagtgg cactggttgc
      721 ctgtatgcgt aagttgctga ccattatgaa cgcaatgctc agaaaaaacg aagaatggaa
      781 tgaatcgtat ttatgaaaac tggattgtgg gccataaata ttccaaaaag atagcccgct
      841 ccgtttatta ccggaacggg cgagagcatg acattgtctg taattataat gagactgctg
      901 attctactta tttttaattt caagaaaagc gttttctaca aagaattatg a
//
LOCUS       DAZOZD010000572          947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZOZD010000572 DAZOZD010000000
VERSION     DAZOZD010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.580"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..947
                     /locus_tag="ACHUPB_004818"
                     /pseudo
     CDS             1..947
                     /locus_tag="ACHUPB_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          233 c          287 g          193 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctg
//
LOCUS       DAZOZD010000573          947 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZOZD010000573 DAZOZD010000000
VERSION     DAZOZD010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.581"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..216
                     /locus_tag="ACHUPB_004819"
     CDS             <1..216
                     /locus_tag="ACHUPB_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0761365.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(161..298)
                     /locus_tag="ACHUPB_004820"
     CDS             complement(161..298)
                     /locus_tag="ACHUPB_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR0761366.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(271..369)
                     /locus_tag="ACHUPB_004821"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="ACHUPB_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(358..597)
                     /locus_tag="ACHUPB_004822"
                     /pseudo
     CDS             complement(358..597)
                     /locus_tag="ACHUPB_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            696..947
                     /locus_tag="ACHUPB_004823"
                     /pseudo
     CDS             696..947
                     /locus_tag="ACHUPB_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S protein"
BASE COUNT          278 a          226 c          202 g          241 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcgga
//
LOCUS       DAZOZD010000574          944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZOZD010000574 DAZOZD010000000
VERSION     DAZOZD010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.582"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(161..832)
                     /gene="queE"
                     /locus_tag="ACHUPB_004824"
     CDS             complement(161..832)
                     /gene="queE"
                     /locus_tag="ACHUPB_004824"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0761367.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          251 a          232 c          210 g          251 t
ORIGIN      
        1 ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa tgattagccc
       61 tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga gtagaagtct
      121 ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata tttagatatt
      181 tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg cacaaacgtg
      241 tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta tcatcggtca
      301 gcgttgccaa tagttcatcc agtgcttcaa tatcgcgtac gcgccccact ggatgcttga
      361 tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc atgttcagct
      421 ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta ccactggttt
      481 cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggtagc aaatcatgaa
      541 tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg cgactaatga
      601 cagccaacaa gtcttcactg ctcgccgccc cccacttatc actctctttg gtcttcgcca
      661 gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc
      721 accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg acgccggtaa
      781 agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc atagcattct
      841 ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat gtcttattaa
      901 cattctgtta caggcagatt taaagaaaaa tgcgaaacat atcg
//
LOCUS       DAZOZD010000575          942 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZOZD010000575 DAZOZD010000000
VERSION     DAZOZD010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.583"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            192..>942
                     /locus_tag="ACHUPB_004825"
     CDS             192..>942
                     /locus_tag="ACHUPB_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR0761368.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQ"
BASE COUNT          207 a          291 c          246 g          198 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      781 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      841 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      901 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gc
//
LOCUS       DAZOZD010000576          940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZOZD010000576 DAZOZD010000000
VERSION     DAZOZD010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.584"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUPB_004826"
     CDS             complement(<1..228)
                     /locus_tag="ACHUPB_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR0761369.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHUPB_004827"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHUPB_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..>940)
                     /locus_tag="ACHUPB_004828"
     CDS             complement(586..>940)
                     /locus_tag="ACHUPB_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761370.1"
                     /translation="PSRLERLSRFQFIIYTLTSWSILITLNDINLALSFILVINNTYV
                     NINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTI
                     MLSAIPSAKMSLSAH"
BASE COUNT          307 a          164 c          182 g          287 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg
//
LOCUS       DAZOZD010000577          934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZOZD010000577 DAZOZD010000000
VERSION     DAZOZD010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.585"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPB_004829"
     CDS             complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUPB_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0761371.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPS"
     gene            complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPB_004830"
     CDS             complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUPB_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0761372.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          268 a          219 c          190 g          257 t
ORIGIN      
        1 cgaaggttct aatcgtgata ctggcggaat aacggtaaac ggcgctttgc ttttatcctc
       61 tttattaaat ggatctaccc aagcctggat gagataaggt attttttctt ctttgctaaa
      121 tacagaaata gatttatcag cattattact taagtaaacc acccgcgtac caccaatgac
      181 aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa
      241 taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc tggacataac
      301 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
      361 acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt ggttgccaca
      421 tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt ataagcagag
      481 tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat accaacgccg
      541 gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc
      601 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
      661 gcttgagtgt acgttgccgg gcaattttca agtttaatac tgaacttctg tggcgacgcc
      721 agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac aatttgatcg
      781 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
      841 ccatcttctg ccagtgcatg cccggccagc attagagatg ccagagtgaa tgcaccgcct
      901 ataatattac gcttcataaa aactccattg atat
//
LOCUS       DAZOZD010000578          934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZOZD010000578 DAZOZD010000000
VERSION     DAZOZD010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.586"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(196..>934)
                     /locus_tag="ACHUPB_004831"
     CDS             complement(196..>934)
                     /locus_tag="ACHUPB_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0761373.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          258 t
ORIGIN      
        1 tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg ttgatgactt
       61 gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat ttttaaggat
      121 tatcctgtct atgattttct acttagaagg atatggcact aactatgcat cttatagttt
      181 actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga tagccatacc
      241 taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt taagtaatat
      301 cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg ctcccagacc
      361 cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc ggatatacag
      421 cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc ctgcatgagc
      481 ggcaatccac gctgagggaa taaagccata aataaaagat cctattaata ctcctaacaa
      541 aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta gttttatggg
      601 attaatactt actacagctt tggtactgca acaggtaact acggggttct caatggcttt
      661 agccgctgga ctacaacaac tcacttttat gggcgtatat gtcgttcccg gcgaagtttt
      721 tgctggagca caacaatttg ctgatgagct tttactggca atgatatgac gttcaaaccc
      781 cagagaatcc aggataatac tggcaagaac ggagacgccg gctgcgataa tcgcgtacaa
      841 caaggtgact ttccaaccaa aggtcatcca cattagtccg acgataatgg gattaagtaa
      901 tggtgaaaca aataaaaaag tgagagtcgg acca
//
LOCUS       DAZOZD010000579          928 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZOZD010000579 DAZOZD010000000
VERSION     DAZOZD010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.587"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..184
                     /locus_tag="ACHUPB_004832"
                     /pseudo
     CDS             1..184
                     /locus_tag="ACHUPB_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(201..899)
                     /locus_tag="ACHUPB_004833"
                     /pseudo
     CDS             complement(201..899)
                     /locus_tag="ACHUPB_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          237 a          221 c          245 g          225 t
ORIGIN      
        1 tttaatacga gggaggccgg cagaaaaacg gcttgcggta aggcaggaga aatgtgtgcc
       61 gcgaatgaag tccttgtatg actggataaa gctccagagg aaaactttgt caaaccatgc
      121 tgaaatggta aaggcgttca actatatcct gaatcactgg aatgcactga atgagttttg
      181 ccgggtagta cttccaatca ttagaatatg tgcttttcaa tgaaggaacc gatgactttt
      241 tcgtggattt ccacggaacg cgagaagcag attgttttac gtgccagccg cttgatgcgg
      301 gggcggaggg tcaggttgtt acgttcatac gttgcgtgaa gattttgccg gtcaggtgtt
      361 tctcctccgg cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccaccat
      421 gccgatacag aacggagtca acagggctaa cagttcccga caggtctcat cattccgtgg
      481 cccgcaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc agggccagtg
      541 ttgttgcgcc ttgctgccaa cgaagcgcca ctgctcatca agttcacaga taagggcaac
      601 atcagcgtga gcgactggcg cagatgttat ccgccggggc gagagctttt taatgtccgt
      661 atgacggtat tgatgccgat tttcagtgtt ctggcggtat aacagccccc tgcgccgtta
      721 tgtgccattt caacgatctg ctccttaacg ccgggttttc tggcttcata agagtaagtg
      781 agctggaaaa cacgtttaca tgagcgacaa cggaaacgct catgccggga acaactacgg
      841 ccatgtcgat aaacttcatc ggaatgacag cgcggacaat gaacagtaac ggtagtcatg
      901 gaaaacctca aataccagta gattacca
//
LOCUS       DAZOZD010000580          901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZOZD010000580 DAZOZD010000000
VERSION     DAZOZD010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.588"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..778
                     /locus_tag="ACHUPB_004834"
     CDS             <1..778
                     /locus_tag="ACHUPB_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0761374.1"
                     /translation="HRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHN
                     KAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGEL
                     ATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTV
                     NGEWQADEYGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYT
                     VNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            803..>901
                     /locus_tag="ACHUPB_004835"
     CDS             803..>901
                     /locus_tag="ACHUPB_004835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFR0761375.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAEE"
BASE COUNT          257 a          219 c          236 g          189 t
ORIGIN      
        1 tcatcgccag gaaatggccg aaacagtatg gaaagaatta aaagcaactg actctgcaac
       61 gtgccgtagt tgccattctt ttgatgccat ggatattgcc tcgcaaagtg aatctgcgca
      121 gaaaatgcat aacaaagcac aaaagggcgg cgaaacctgt atcgattgtc ataaaggcat
      181 tgcccatttt ccgccagaaa taaaaatgga tgacaacgcg gcgcatgagc tggaaagtca
      241 ggccgctact tcagtgacta atggcgcaca tatttatcct ttcaaaactt ctcgcatagg
      301 cgagctggct accgtgaatc ctggtaccga tctcaccgtc gttgatgcca gtggcaaaca
      361 gccgatcgtt ctgttgcagg gttatcaaat gcagggcagt gaaaacacgc tctacctggc
      421 ggcaggtcaa cggctggcgc tagccacatt aagtgaagaa ggtatcaagg cgctcacggt
      481 aaacggggaa tggcaggctg acgaatacgg caatcaatgg cgtcaggcgt ctttacaggg
      541 tgcgcttacc gatcccggat tagcggaccg taaaccgcta tggcaatacg ctgaaaaact
      601 tgacgatacc tattgcgctg gttgtcatgc ccctattgcc gccgaccatt acaccgtcaa
      661 tgcgtggccg tccattgcca aaggaatggg ggcacgaacc agcatgagcg aaaacgaact
      721 ggacatttta acgcggtatt tccagtacaa cgccaaagat attaccgaga aacagtgatc
      781 ctcgttgatc agggaggaag ttatgacatt aacaagacgt gaatttatta aacacagcgg
      841 tattgccgct ggagcgcagg tggtgacatc ggcagcaccg ctccccgcat gggcagaaga
      901 g
//
LOCUS       DAZOZD010000581          895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZOZD010000581 DAZOZD010000000
VERSION     DAZOZD010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.589"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            53..895
                     /locus_tag="ACHUPB_004836"
                     /pseudo
     CDS             53..895
                     /locus_tag="ACHUPB_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085938460.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    283..399
                     /locus_tag="ACHUPB_004836"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          200 a          216 c          274 g          205 t
ORIGIN      
        1 ggatgactaa aaatactcgt tttcccccga agtccgtcaa cgggcagtcc gtatggttct
       61 ggaaagtcag ggcgaatatg actcacaatg ggcggtaatt tgttccatta ctccaaagat
      121 tggctgtaca ccggagactc tgcgtgtctg ggttcgccag catgagcggg ataccagggg
      181 cggtgatgga gggctcacca ccgctgaacg tcagcgtctg aaagagctgg agcgtgagaa
      241 tcgtgaactg cgctgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc
      301 ggagttcgac cgcctctgga aaaaataatg ccactgctgg ataagctgcg tgggcagtac
      361 ggggtcggac cggtatgcag tgaactgcat attgccccgt caacgtatta ccactgtcag
      421 caacaggaca tcatcctgat aaacgcagtg cccgtgcgca gcacgatgac tggctgaaga
      481 gagagataca gcgcgtatac gatgaaaatc atcaggtgta cggtgtgcgt aaagtctggc
      541 gccagttgtt acgggaagga atcagggtgg ccagatgtac agtggcgcgc ctcatggcgg
      601 ttatgggact tgtcggtgtt ctccggggta aaaaggtccg cactaccgtc agccggaaaa
      661 ccgttgccgc aggtgaccgc gtaaaccgtc agttcgtggc agaacgtcct gaccagctgt
      721 gggtggctga ttttacttac gtcagcacat ggcagggctt cgtctatgtg gcgttcatca
      781 ttgatgtgtt tgccggatac atcgtgtggt gacgagtctc atcgtctatg gaaacgacat
      841 tcgtgctgga tgcactggag caggcgttgt gggcccgtcg tccgtctggc accat
//
LOCUS       DAZOZD010000582          894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZOZD010000582 DAZOZD010000000
VERSION     DAZOZD010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.590"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..398
                     /locus_tag="ACHUPB_004837"
     CDS             <1..398
                     /locus_tag="ACHUPB_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFR0761376.1"
                     /translation="KECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFN
                     GLGISVHDISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRI
                     WFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            395..874
                     /locus_tag="ACHUPB_004838"
     CDS             395..874
                     /locus_tag="ACHUPB_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR0761377.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
BASE COUNT          314 a          158 c          137 g          285 t
ORIGIN      
        1 tcaaagaatg tatcgaaaat ggcattaatt caactccctc gacaggatac caacctctta
       61 tcctgcaaag cattaaaacc aaattacttt catcaagact aaataaattt aatgacttcg
      121 aggacagttt taatggctta ggaatcagtg ttcatgatat ttcagctcaa aaaatatctc
      181 ttttaagttt tcaaaaatac gctataggat ggagtgcaac aattcatttt gtagcacagg
      241 accacttcgg tcttgatgta actgatatca aaaacaagac ttacagcaaa tatagatttt
      301 ttcgcatatg gttcttttta caacggcata aagactttgc tttcaaacct tttttcacca
      361 attttaacac tatcgaaaga atcgaaaatt atctatgaaa agcaaactcc agaaaattat
      421 attatgttta tttcttctat gctgcatcta taatttatgg acattgcgtc ctgtacagat
      481 tttgtataca tactctgatg ctggcaattc agtctttctg gtcgttgatc atctgccatg
      541 gactgacagc gataaaataa attggtatct gaagcatcag aatgaaatta aaaatcaaca
      601 tcccctacca gaaggtagtt ggcatacctg gtatgtcatt gatatcggta atggcttcac
      661 tgactataaa aaatatatcg aagggccgta tgaagatctc tactgtttcc cgacaattaa
      721 gagcaatgat aattgcatcg tgaaaaatta cttaatggtt attaatgaat acccttatcg
      781 taacactcat attggtatta acgattttac tgagtatcaa ttaacacaag aaaataaaat
      841 agagcgagtt ttcaatccgc atgactttaa ataagacaat ttttagaaat aagc
//
LOCUS       DAZOZD010000583          886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZOZD010000583 DAZOZD010000000
VERSION     DAZOZD010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.591"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUPB_004839"
     CDS             <1..203
                     /locus_tag="ACHUPB_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761378.1"
                     /translation="KRIRECNLDSQGAIYLCESAKAGAVLIFWHELAINGYASMNAIK
                     RQELIDADFQRLRNLIWPEDDR"
     gene            203..793
                     /locus_tag="ACHUPB_004840"
     CDS             203..793
                     /locus_tag="ACHUPB_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761379.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
BASE COUNT          189 a          251 c          289 g          157 t
ORIGIN      
        1 aaaaacgcat ccgggaatgc aatctggaca gccagggcgc gatttatctc tgcgagtcag
       61 ccaaagccgg agccgtgctg atcttctggc atgaactggc cataaatggc tatgcctcaa
      121 tgaatgccat aaagagacag gagctcattg acgccgactt tcagcgcctc agaaacctca
      181 tctggccgga ggatgacagg taatgaccca gagccgccga ccgtcgccac tgcagcggag
      241 agtgctgatt gtgctggctg cccttgatga aaaacggccg ggacccgtgc tgacgcggga
      301 cctggaacgg gtgctggagc gaagcgggga agccccggtc tacgggccaa acctgcgcgc
      361 ctcctgccgt cgtctggagg atgccggctg gctgcgtacc ctgcgcgctc cgaacctgca
      421 gctggcagtg gagctgacgg acgccggccg ggccgtggca cagcctctgc tgctggcaga
      481 acaggaccgc attcgggcag aacagcgtgc ggcagaagtc gtcgtactgc cgctggtccc
      541 ggcagcaggt ctgcccgcag acggcacttc tgccactgat ctggcggtgg agctgaacgg
      601 cataacgtat caggcctgcc gtggggattt tgtggtacgt ctcgacggca gcacctgtct
      661 gcagttatgg aataaagaag gcagggtggt acgccgggag ggcgatccgc tggaagtggc
      721 gcagtggctg caggcctgtc acgatgccgg aatggaggtt cgtgtacagg ttaatgaaag
      781 cgtcaccccg taaaaacata ccagcacggt ttacacgtta tcccatggtg gcagccggtt
      841 aactttcctg cgccaccagt ggaaagaggc catgagcagt ccgaac
//
LOCUS       DAZOZD010000584          886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZOZD010000584 DAZOZD010000000
VERSION     DAZOZD010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.592"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..67
                     /gene="ykiE"
                     /locus_tag="ACHUPB_004841"
     CDS             <1..67
                     /gene="ykiE"
                     /locus_tag="ACHUPB_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR0761380.1"
                     /translation="GWSLCFWHVSVRMTHDVVCYY"
     gene            154..851
                     /locus_tag="ACHUPB_004842"
                     /pseudo
     CDS             154..851
                     /locus_tag="ACHUPB_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT          210 a          217 c          237 g          222 t
ORIGIN      
        1 gggatggagc ctctgcttct ggcatgtgtc ggtcagaatg actcatgatg tggtctgcta
       61 ttattgacat cctcactgcc ctaaaggata gggatttcgg taatgctgtc aacttactga
      121 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt ccatttccat cagatgtcct
      181 tcctgctccg ctactgaagg cgtggtgcgt aacggcaaaa gcactgccgg acatcagcgc
      241 tatctctgct ctcattgccg taaaacatgg caactacagt tcacttacac cgcctctcag
      301 cccggtaagc accagaaaat cattgatatg gccatgaatg gcgtcggatg tcgcgccagt
      361 gcacgcatta tgggcgttgg cctcaacacg gttttacgtc acttaaaaaa ctcaggccgc
      421 agtcggtaac ctcgcgcata caaccgggca gtgatgtgat tgtctgcgct gaaatggacg
      481 aacagtgggg ctacgtcggt gctaaatcac gtcagcgctg gctgttttac gcgtatgaca
      541 ggatacggag gacggttgtg gcgcacgtct tcggtgaacg cactctggcc acactggagc
      601 gtcttctgag cctgctgtcg gcctttgagg tcgtggtata gatgacggat ggctggccgc
      661 tgtatgaatc acgcctgaag ggaaagctgc acgttatcag caagcgttac actcagcgca
      721 ttgagcgaca taatctgaat ctgagacaac atctggcaag gctgggacgg aagtcactgt
      781 cgttctcaaa atcggtggag ctgcatgaca aggtcatcgg gcattatctg aacataaaac
      841 actatcagta agttggagtc attacctcct cccgaactca aaacta
//
LOCUS       DAZOZD010000585          882 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZOZD010000585 DAZOZD010000000
VERSION     DAZOZD010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.593"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUPB_004843"
     CDS             complement(<1..680)
                     /locus_tag="ACHUPB_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0761381.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          265 a          143 c          183 g          291 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt ac
//
LOCUS       DAZOZD010000586          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZOZD010000586 DAZOZD010000000
VERSION     DAZOZD010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.594"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUPB_004844"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUPB_004844"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0761382.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAZOZD010000587          876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZOZD010000587 DAZOZD010000000
VERSION     DAZOZD010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.595"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>876
                     /locus_tag="ACHUPB_004845"
     CDS             <1..>876
                     /locus_tag="ACHUPB_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR0761383.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKN"
BASE COUNT          313 a          176 c          161 g          226 t
ORIGIN      
        1 tgtatataat attaacgtta cgtcaccttc cggacagcaa gaaacattta aaatactaat
       61 caatccagaa catctacggc agacacttga gcgtaaatct cttactgctg ttcagaaatc
      121 acaatgtgaa atcatcaccc ctaaaaaacc cggcgaagcg attcttcatg cttttaatgc
      181 cacctaccag caaatcaggg aaaatatgtc tgagtttgca cgttgccatt atgggtatat
      241 acaaatccct ccagtgacaa ctttccgcgc cgacggacca gaaactcccg aagaagaaaa
      301 gggttactgg tttcatgctt atcaacccga agatctttgt accatccata atccaatggg
      361 agatttgcag gattttattg cattggttaa agatgctaaa aaatttggta tcgatatcat
      421 tcctgattat acctttaact ttatgggaat tgggggtagt ggtaaaaatg acctggatta
      481 tccctctgct gatatacgag cgaagatcag taaagatata gaaggtggta tccctggcta
      541 ttggcaaggt caggttttga ttccattcat taaagatcca gtaacaaaag aacgtaaaca
      601 aatccatcca gaagatatac atctcactgc aaaagacttc gaagcaagta aagataacat
      661 ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaattcc
      721 taaaacaaca cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat
      781 gcgaaaatat ggcgtacgag gtttacgtta tgatgcggca aaacactcaa aacatgaaca
      841 aatagaaaga tcaataaccc caccgcttaa aaatta
//
LOCUS       DAZOZD010000588          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZOZD010000588 DAZOZD010000000
VERSION     DAZOZD010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.596"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            235..804
                     /locus_tag="ACHUPB_004846"
     CDS             235..804
                     /locus_tag="ACHUPB_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0761384.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
BASE COUNT          273 a          166 c          199 g          237 t
ORIGIN      
        1 caccagctat gtgaccccct ttgcaaaaaa gccaaatatc attgactttc tcggtgaatt
       61 gctataaagg catctatcta ggtctttaac tgtataaata cacatgacaa caaacgcccg
      121 ttaagtgtct atataaaaag gtagctgtag ctatagacaa caattgtgat aattgattta
      181 atggctatag acaagcaaga cagacctttg ttgactatat agagagataa gaacatgcgt
      241 acattccttt atgctcgtgt atcagttgat gacctcacca cacagaacca agtagaagct
      301 gttaaacgtg ctggttatga agtcagacct tcacgagtaa ttgaggaagt tattagtggg
      361 gctacaccag ctatggacag accacaattt gtaaagctgg tggataagct ggaagaaggt
      421 gattgtcttg tagtgaccaa gattgaccgc ttaggaaggg ataacattga cgtacagaag
      481 accgtcacaa tgttacttgg tatgggtgtt aagctggtct gtctagattt gcctgtccaa
      541 gacctttcaa gtgctgaagg taaacttatt cttcaactat tcagtacctt tgcagagttt
      601 gaacttaacc gcatcagaga gcgaaccaga gagggcttag aacgagctaa agcacaaggt
      661 aaggtgttgg gtcgtcctgt agctaaaaac actacagagg ccgttttaga gcataaggct
      721 aagggattgt cacaaagtca ggttgcaaag cttctgaatc tctctgtgag aacggttagc
      781 cgtcactgga caaagacact gtaaaaaaaa cttagtagca tcttcatagc ctccttcatg
      841 ggggcttttt tgtgtctgta acatatggtg accaa
//
LOCUS       DAZOZD010000589          875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZOZD010000589 DAZOZD010000000
VERSION     DAZOZD010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.597"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>875
                     /locus_tag="ACHUPB_004847"
     CDS             <1..>875
                     /locus_tag="ACHUPB_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0761385.1"
                     /translation="GRYELTIAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPR
                     QTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIA
                     RDAHYLYRYDHHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLV
                     ESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQT
                     IYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLES
                     EILADRVSEESRRWL"
BASE COUNT          197 a          282 c          263 g          133 t
ORIGIN      
        1 cggccgttat gaactcacca tcgcttatac ccctgccggg cagttacaga gccagcacct
       61 gaacagcctg ctgtctgacc gcgattacac ctggaacgac aacggcgaac tcatccgcat
      121 cagcagcccg cgccagaccc ggagttacag ctacagcacc accggcaggc tgaccggcgt
      181 tcacaccacc gcagcgaatc tggatatccg catcccgtat gccacagacc cggcggggaa
      241 ccgcctgccc gacccggagc tgcacccgga cagcaccctc agcatgtggc cggataaccg
      301 tatcgcccgt gacgcgcact atctttaccg gtatgaccat cacggcagac tgacagagaa
      361 aaccgacctc atcccggaag gggttatccg cacggatgat gagcgcaccc atcggtacca
      421 ttacgacagt cagcaccggc tggtgcacta cacgcggaca caatatgcag agccgctggt
      481 cgaaagtcgc tatctttacg acccgctggg ccgcagggtg gcaaaacggg tatggcggcg
      541 tgaacgggac ctgacgggct ggatgtcgct gtcacggaaa ccgcaagtga cctggtacgg
      601 ctgggacggc gaccggctga ccacaataca gaacgacaga acccgcatcc agacgattta
      661 tcagccgggg agcttcacgc cactcatcag ggtcgaaact gccaccggtg agctggcgaa
      721 aacgcagcgc cgcagcctgg cggatgccct tcagcagtcc ggcggcgaag acggtggcag
      781 tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg ctggaaagtg aaatcctggc
      841 tgaccgggtg agtgaggaaa gccgccgctg gctgg
//
LOCUS       DAZOZD010000590          874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZOZD010000590 DAZOZD010000000
VERSION     DAZOZD010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.598"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..835)
                     /locus_tag="ACHUPB_004848"
     CDS             complement(<1..835)
                     /locus_tag="ACHUPB_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFR0761386.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EV"
BASE COUNT          184 a          234 c          247 g          209 t
ORIGIN      
        1 tcacttcatt tcccagtccc ggccactttt ttcgtgcctg cgggctggtt ttcagcttca
       61 ccagatgatc gcctttaccc agttttctga gctcttcata ttgcgctccc tttctgagag
      121 gtatcatcca gtggcggtgt tctcccgcca ggctccaggc atttaacagt cccagtgagt
      181 aataaccttt atccattaac gtcagagtgt tatcgccggt ttgttctata agttgctcag
      241 caagctcatt ttcgctgttc ttcatcgtgc cgaaggctgc agccgtcagc agatggctgg
      301 tcagttccat ctggcagacc attttgacct gcgggtagag cgccgggttc ccggcatgtg
      361 tctggcgggg gaaggctgca tcgttctctg gtgtatccgg tgtgcgccag aacacaccat
      421 cgatggccag cagggtcagg ccgcaccagt gcggatgcgg cgtggcgtta tgccagagct
      481 gcgctgtttt cgtgaacacg cggcggacag cctcacttcc caggcgctgg cgggcctgaa
      541 taacggcact gggggcaacg aaggggcgat tgcccggcag catgatgtcc aggcgattca
      601 caatctggtg aagaggttct ttacgctcaa gcgccatgcc aacaatacac cagaccatca
      661 tttcgagggg aagacggcgc ttgcgtagcg ttacagtacc tgattcggca aggcaacgag
      721 agatgagttc ggggtcgagg taatccccca gagaagtcag tgggttacgc agagaatcgt
      781 aacgggatac cagatcaaga gcctgtccaa tgtgcataaa aaaatccgga aacaagtgag
      841 cgtttccgga ttcttacaca gccactggat cggt
//
LOCUS       DAZOZD010000591          872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZOZD010000591 DAZOZD010000000
VERSION     DAZOZD010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.599"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            35..732
                     /locus_tag="ACHUPB_004849"
     CDS             join(35..284,284..732)
                     /locus_tag="ACHUPB_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR0761387.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
BASE COUNT          205 a          222 c          228 g          217 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactatcaat aagttggagt cattaccaac tcatcttctg gttttatttc catgctcctc
      781 tcctttgatg cgaataccag cggtaattga agcctgatag ctaattttac tcacagtacc
      841 gcctcctgaa atttcccctg ataaaacgcc ag
//
LOCUS       DAZOZD010000592          854 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZOZD010000592 DAZOZD010000000
VERSION     DAZOZD010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.600"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>854
                     /gene="ospD3"
                     /locus_tag="ACHUPB_004850"
     CDS             <1..>854
                     /gene="ospD3"
                     /locus_tag="ACHUPB_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR0761388.1"
                     /translation="IFVDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTR
                     VVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVE
                     LLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVH
                     RQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNG
                     QADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHT
                     ADLTPHQA"
BASE COUNT          281 a          167 c          173 g          233 t
ORIGIN      
        1 ctatatttgt ggacagacat actccaacaa gcatgtcctc cataatcaga tggcctaata
       61 atttacttca ccccaaagtt atttatcacg cgatgcgtat gggattgact gagctaatcc
      121 aaaaagtaac aagagtcgta caactatctg acctttcaga caatacgtta gaattacttt
      181 tggcagccaa aaatgacgat ggtttgtcag gattgctttt agctttacaa aatgggcatt
      241 cagatacaat cttagcatac ggagaactcc tggaaacttc tggacttaac cttgataaaa
      301 cggtagaact actaactgcg gaaggaatgg gaggacgaat atcgggttta tcccaagcac
      361 ttcaaaatgg gcatgcagaa actatcaaaa catacggaag gcttctcaag aagagagcaa
      421 taaatatcga atacaataag ctgaaaaatt tgctgaccgc ttattattat gatgaagtac
      481 acagacagat acccggacta atgtttgctc ttcaaaatgg acatgcagat gctatacgcg
      541 catacggtga gctcattctt agcccccctc tcctcaactc agaggatatt gtaaatttgc
      601 tggcctcaag gagatatgac aatgttcccg gacttctgtt agcattgaat aatggacagg
      661 ctgatgcaat cttagcttat ggtgatatct tgaatgaggc aaaacttaac ttggataaaa
      721 aagcagagct gttagaagcg aaagattcta atggtttatc tggattgttt gtagccttgc
      781 ataatggatg tgtagaaaca attattgctt atgggaaaat acttcacact gcagacctta
      841 ctccacatca ggca
//
LOCUS       DAZOZD010000593          853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZOZD010000593 DAZOZD010000000
VERSION     DAZOZD010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.601"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..382
                     /locus_tag="ACHUPB_004851"
                     /pseudo
     CDS             1..382
                     /locus_tag="ACHUPB_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            563..712
                     /locus_tag="ACHUPB_004852"
                     /pseudo
     CDS             563..712
                     /locus_tag="ACHUPB_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          223 a          172 c          257 g          201 t
ORIGIN      
        1 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
       61 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      121 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      181 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      241 tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg tgcggaggtc agatgcggtt
      301 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      361 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      421 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      481 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      541 aatcttttgg ggatttaaat tcctatcgat ggacatgaat gcaggctata taagagcagc
      601 gcgcatccac ttacccaatg cggttgagaa aatagccttc gatcgctttc atgtggcgaa
      661 acaaccgggt gaggtggtgg ataaaacccg ccagaatgaa ccgccccggg tttcctggag
      721 agtgttttat ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc
      781 tttttctgct tctgccggag gagtatggcc cagcctttcc agcaatcgtc gattgttata
      841 ccagtccacc cac
//
LOCUS       DAZOZD010000594          841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZOZD010000594 DAZOZD010000000
VERSION     DAZOZD010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.602"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            13..813
                     /locus_tag="ACHUPB_004853"
                     /pseudo
     CDS             13..813
                     /locus_tag="ACHUPB_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          238 a          165 c          207 g          231 t
ORIGIN      
        1 agggaataca taatgaaaag aaaagttctg gcaatgctgg tcccagcgtt attagttgct
       61 ggcgcagcaa atgcggctga aatttataat aaaaatggca ataaactgga tctgtacgga
      121 aaagttgatg ctcgtcatac tttttccgat aaccctggcg atgacggcga tgaaactatc
      181 atcgagttag gcttcaaagg tgagacccaa attacagatc agttaactgg ttatggtcaa
      241 gcactgacca aaacaaaggc tagcgacact gaaggttctg ataatactta tgtcaaactt
      301 gcttttgcag gtctgaagtt cggcgagatg ggctcctttg actatggtcg taactatggt
      361 gtgatttacg acgttgaagc ctggacagat atgcttcctg tattcggcgg cgactcctat
      421 acctggactg ataacttcat ggcaggccgt gcaaatgggg tagctactta ccgcaatagt
      481 gatttcttcg gtctggtgga aggtctgaac tttgcattgc agtatcaagg taacaacgaa
      541 ggcagcaatg caggagaaga tcaagaaggt actaaaaatg gtcacgaaga cgttcgcttc
      601 cagaacggtg atggttttgg tctttccact tcatatgact ttgacttcgg tttgagctta
      661 ggtgccgctt actcaaactc tgaccgtacc aattctcaag tagctcttgg cgggtatcac
      721 tacaatgaat acagcaaatt tgctggtggt gatacagctg aagcatggac atttggtgca
      781 aaatacgacg ccaacaatgt ttatcttgca atgggtgatg ctgccaactt actgatttag
      841 t
//
LOCUS       DAZOZD010000595          831 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZOZD010000595 DAZOZD010000000
VERSION     DAZOZD010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.603"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            115..>831
                     /locus_tag="ACHUPB_004854"
     CDS             115..>831
                     /locus_tag="ACHUPB_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761389.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELVEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILECLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARR"
BASE COUNT          218 a          222 c          187 g          204 t
ORIGIN      
        1 ttttaaaaga caccaccaga acaactccct tccgttttca cttccaaaaa ctgacaccag
       61 tcgctatcat ccgtccattg tgataagtaa gtaactacct accaggtgag ccacatgagc
      121 caaatctttt tcgataccat cgacaacgac cagtacgact tcatgacaga gtggaatacc
      181 gctgttatgg acaagtgggt cgctgaaaac attggtttgt cgcgctgtaa agacgaggct
      241 gaactcttcg agacgaagtg gtttgattac cgagacatgc atcctcttat ggccacctgt
      301 ctgtttacgg aggcatacaa acgtcagtac tcaaatatca tgctgacgca cggtcgcgaa
      361 cactttgaaa cagctccgtt caccaccggg ttaaaacgcc tgccttatca ggagttgtcg
      421 actgccaata aaacgtctct atggaaagca cgccagtttg ctgaccgcta ttgctgctca
      481 tacgactact ttatctccac cgttctttcc gcagctgcac gacggctgtg ggacaagctg
      541 ccgcgcccac agcatttgtg gcagccagag ctggttgaga tattcgaaga gaaattagcc
      601 agacgcgcaa caacccgtct ggatgactct cttgttagct ttaagcatat gggagacatg
      661 cagttcaacc cgattcagga aagctatttt gagtggattc tggagtgttt gcgcaccatc
      721 cctcgcagca agcgcatacg cgcaattttc tccgctatct ggctaatgga aatcgttcca
      781 gagcgcctta tttccgccca ctttccagaa gaactggaag aagcacggcg g
//
LOCUS       DAZOZD010000596          818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZOZD010000596 DAZOZD010000000
VERSION     DAZOZD010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.604"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(130..>818)
                     /locus_tag="ACHUPB_004855"
     CDS             complement(130..>818)
                     /locus_tag="ACHUPB_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0761390.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
BASE COUNT          229 a          172 c          189 g          228 t
ORIGIN      
        1 ggatatggaa taatattaat tgaggtgaat gaccagaaat atcaaaaatt gctcattttt
       61 acttaaggag tcatacagca gcacttaaat caaaaaccag cccagagaag ggctggtcag
      121 gagaggacgt tagaactgat aggtaatgcc cgtagcgacg atgttatcgg tccgaatatc
      181 tacggcacgg gtaaagtcgc tttcatcaat caggttgatt ttataatcaa cataggtcga
      241 catatttttg ttgaagaaat acgtcgcacc aacgtcgata tactcaatca tgtcctggtc
      301 gccgtagcgg cccagatctc tgcctttcga tttcaggtaa gcgagtgatg ggcgcaagcc
      361 gaagtcgaat tgatattgcg ccaccacttc aaaactttgt gctttgttag agatataacc
      421 atccccatag gtggtcatgt ttaatgtttc agcgtaatta gctgcaaagt agatgttgtt
      481 ggcatcatac tttatacctg tggcccatag ttctgcattc ttaccggagg cattgagagg
      541 attagcagcg gctctttcct gcgcatttgt ccgatcggat ttggtatata ccgcaccgat
      601 accaaagcca tcataaacat aagttgagga gataccgaaa ccgtcaccgt tggcacgcgt
      661 gtagtcagca ccataaagat gactgttgtc agtacgttca tttttgcctt gatattgcgc
      721 ggcaaagttc agcccttcaa ttaagccaaa gaaatcgtag ttgcgatagg ttgctagacc
      781 ggtggtacgg tatgtcatga agacatcttt ctgactcc
//
LOCUS       DAZOZD010000597          797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZOZD010000597 DAZOZD010000000
VERSION     DAZOZD010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.605"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            68..361
                     /gene="mxiI"
                     /locus_tag="ACHUPB_004856"
     CDS             68..361
                     /gene="mxiI"
                     /locus_tag="ACHUPB_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR0761391.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            367..>797
                     /gene="mxiJ"
                     /locus_tag="ACHUPB_004857"
     CDS             367..>797
                     /gene="mxiJ"
                     /locus_tag="ACHUPB_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR0761392.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKP"
BASE COUNT          266 a          118 c          166 g          247 t
ORIGIN      
        1 tacagtgaaa gtgattaagg atgttgatgc tgcaattatt caaaacttca gataataggg
       61 agcattcatg aattacattt atccagtcaa tcaggttgat attatcaaag ccagtgattt
      121 tcaatctcaa gagatatcaa gtctggaaga tgtcgtgtcg gctaaatata gtgatattaa
      181 gatggataca gatattcaag tatcacaaat aatggagatg gtaagcaatc cagaatcatt
      241 aaacccagaa tctttggcca agttacagac gacgctctca aattattcaa taggagtatc
      301 attagctggc acgttagcaa gaaaaacagt ttcggctgtt gaaactttat taaagtctta
      361 atttatatga ttaggtataa aggttttatt ttattcttgt tgctgatgtt gattggatgt
      421 gagcaacgtg aagagttaat ttctaattta tctcaaagac aggcaaatga aataatatct
      481 gtgctagaac gccataatat tactgctaga aaagttgatg gaggtaaaca ggggatctcg
      541 gtacaagtcg aaaaggggac atttgcatcg gcagttgatt tgatgcgcat gtacgatttg
      601 ccaaatccgg agagagttga tatctcacaa atgtttccta cagattcatt agtgtcttct
      661 ccaagagctg aaaaggcccg tttatatagt gctattgagc aacggctgga acagtcttta
      721 gtttctattg gtggtgttat ttcggcaaaa atacatgtta gctatgatct tgaagaaaaa
      781 aatatatctt caaaacc
//
LOCUS       DAZOZD010000598          796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZOZD010000598 DAZOZD010000000
VERSION     DAZOZD010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.606"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..742)
                     /locus_tag="ACHUPB_004858"
     CDS             complement(<1..742)
                     /locus_tag="ACHUPB_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR0761393.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          138 c          161 g          231 t
ORIGIN      
        1 cacataaaca ggtaaagacc ataaatatgg tgaaagtaaa aaagaaggaa aatacagcaa
       61 tggaaaaaat tttatcggag aacaggaata aatctaaaag atagattgct gaaatacaga
      121 ctccactttt acatgcgcga caaaaattac tgccttgatg aaaaaaccag aaagggaaga
      181 acatcactgc aaatacccaa aaaatagcaa agtaataatg atcactggaa gacgctgatt
      241 cactttctga aggattagtc ggatattggt gttgtgaagt gagttggtta ttcaaagcgt
      301 ttatagctag ctctaatccc tgagctaatt tttgttgttt aaatgcgggt atcatgttgc
      361 tacggatgat atctccagcc agagcatcgg ttaccttttc ttccagccca taacctacct
      421 ggatgcggac agtacgatct gaccaggcaa caatgatcag tatcccatca ttacgtttgg
      481 catctcctaa acgccaattg tcaaaaactc ttgtagcata ttgttcaatt gtctcgtctt
      541 tggttgtggg aacaattaat acagcaagtt gcacttgggt attattgaaa atagattcta
      601 acttgtgcgt cagttctttc ttttcttgtg agtttaagct actcgtaaga tccgtaactt
      661 gttgctgtaa ttgtgggaca atagttggtt gggctgcaac aaaggaacac acgaaagtga
      721 acattagtat gaaatagcgc attataaatt tctcattaaa tctggataaa taaattgacg
      781 gcggcatcgg taattt
//
LOCUS       DAZOZD010000599          793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZOZD010000599 DAZOZD010000000
VERSION     DAZOZD010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.607"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..429
                     /locus_tag="ACHUPB_004859"
                     /pseudo
     CDS             1..429
                     /locus_tag="ACHUPB_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          189 a          220 c          226 g          158 t
ORIGIN      
        1 cctgtggaaa aaatacggca gtggcgataa cggtactccc ggccgatagt gaatgacctg
       61 tttgcctggc ttgaagagca ggagccgtgc tgtccgcccg acgggccatt aaataaggcg
      121 attaactata ttctgaaccg gcgcgatgaa ctgagctgct tcctgggtga cggagcggtg
      181 cccctggaca acaatatctg cgagcgtgcc attcgcccgg ttgttatggg acggaaagcg
      241 tggctgttcg ccggttcact gatggccgga aaccgggcgg cacagataat gagccttctg
      301 taaacagcaa aacgtaatgg tctggagcca catgcctggc tgacggacgt cctgacgcgt
      361 ctgccggaat ggccggagga gcgactggca gagttgctgc ctcttgaggg atttaccttc
      421 accgggtgag tgatacctgc cgtcaggtgt tcgtgaaccg ggctataacc agcagtcggt
      481 tgttgaacgc ctgacaacag gaaatcagcc aggccagcaa ccacgcggac tgacagagaa
      541 tcagaacctg aatcagcctg aaatcatacc cggggcagta cttcatcacc agtcaccaat
      601 acccgtgtca gtacttcgcg gcctgacgcg atcacccgac tcagtacttc tgagcccgcc
      661 tccggtacca ttctctgtgg gcccgtacac aacaacagaa aagggggccc ggcccggggc
      721 gcaaccgtat tacacttccg gaaaatcgtt atacgcagaa aaatcggatc acgctgtgcc
      781 tgctttcatg taa
//
LOCUS       DAZOZD010000600          777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZOZD010000600 DAZOZD010000000
VERSION     DAZOZD010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.608"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPB_004860"
     CDS             complement(<1..657)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUPB_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0761394.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     H"
BASE COUNT          234 a          123 c          180 g          240 t
ORIGIN      
        1 atgggatgat aatgggttat cactaatatg tattgaacat tcattcctca gattaagaat
       61 gctttccggg atatgactta tctgatttct atcaaaaaaa tactctctga caacgggatt
      121 atttccctca gaaaatgctg gtagctcagt gaggaaatta cgggtcgctc tgagattttt
      181 aagagcctgt gggagcgatg gtaaggagac tatttcgtta tagctgatat tcatagtcaa
      241 taacgaatcg ggtaaagctg gcaggttttc cagctcattg tgttgtacct gaagtgactc
      301 cagcgcagga ggtagcacgg gcaattctga taatttattg ctggcggaat ataatttttt
      361 tagcgtaaca ggcagttcag gtaggttagt taattgatta tatgatacat tgagcagcgt
      421 tatctgagct ggtaagttgt caggtagcga ggacagattt aaacggtcca gtcgaagttc
      481 atcggaatta ttgataagac attctttaag tcgggagaca gcctcatcac gctcttcacc
      541 ggggagcgct tgtttttccc atttatccca tgctgaaaag taatcagcat atgtaccgga
      601 aatagtgtta taaaaagaat tttggggcaa tgaaaagtta ttatttatcg gtaacatgat
      661 gggcatccag ttaaagccgg tggacaaatg gctttgttaa aagaaataag taggagtttt
      721 tatgaagttc attccatgaa atgaacttca gcactttttt attataaaag gacaaac
//
LOCUS       DAZOZD010000601          777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZOZD010000601 DAZOZD010000000
VERSION     DAZOZD010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.609"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>777
                     /locus_tag="ACHUPB_004861"
     CDS             <1..>777
                     /locus_tag="ACHUPB_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFR0761395.1"
                     /translation="YMEYLVTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGG
                     DLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAAL
                     PFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAG
                     IWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKN
                     MGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALL"
BASE COUNT          251 a          151 c          152 g          223 t
ORIGIN      
        1 ttacatggaa tatctggtaa cttgtcagtt ggatgaacaa caaatgtcat cactgcttta
       61 tgaaagagat gatttaagcg ccattgattt ttcattgctc atgaagacga taaaagcctt
      121 ttcattcggg ggagatctcc taacccttgc atcaaaaccg ggttccacaa tctcaagcat
      181 cccatcagaa agacggatat tgattaacat taaccacgat tttcctaaca atggcaatct
      241 tttcaatgac tttctattta accatcaaca agatgaacaa ttagcaatgg catatatggc
      301 cgctctcccg ttcagcagac ctttagttta ctgggatggc caggtattaa aatcaacgac
      361 tgaaattaaa aattatgatg ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg
      421 ttgctctacc tatcagcagc tctacaatga attccacgca ttatatatag ataaagcagg
      481 aatatggagc gcatttgagg gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg
      541 ggattctgtg aacattaatc actctcctca tgatggacta gttataataa ataaaggaaa
      601 cgaagaagtt gaaggtacct ggcctaacaa attgcaacct ggaatatata aaaacatggg
      661 gagtaatagc gttaacatta ttattaataa tacccgaaaa attatccccc ctggtaaagt
      721 atttacgctt agaggcggaa ctctaaatat caatattcct gggcgtagcg ctcttct
//
LOCUS       DAZOZD010000602          776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZOZD010000602 DAZOZD010000000
VERSION     DAZOZD010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.610"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHUPB_004862"
     CDS             <1..344
                     /locus_tag="ACHUPB_004862"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0761396.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="ACHUPB_004863"
     CDS             complement(406..>776)
                     /locus_tag="ACHUPB_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR0761397.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAZOZD010000603          760 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZOZD010000603 DAZOZD010000000
VERSION     DAZOZD010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.611"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..760
                     /locus_tag="ACHUPB_004864"
                     /pseudo
     CDS             1..760
                     /locus_tag="ACHUPB_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          296 a          131 c          144 g          189 t
ORIGIN      
        1 tcgacaacca agaaccaata gaattacctg cgaactataa agacaaagaa ctcgtccgta
       61 ctatcattaa tgacaacata gttgagaaga ctcatgatat caataacaag gaaatgatct
      121 tcagcgcatt aaaagaaata tatgatggag atcctggttt tatcttcgat aagatatcac
      181 acaaactcag acatacggta acagaatttg atgagagcgg gaatagcgaa ccaacggact
      241 tatttacctg gtacggtaaa gataaaaaag gcaactctct cgctattgta attaaaaata
      301 aaaacggaaa tgattactta tctctcggtt actacgatca ggacgactac cacattcaaa
      361 gaggaattcg tattaatggt gatagtctca cccaatattg tagtgaaaaa gccaggaatg
      421 cttcagcaat gcttcagcgt ggtttgaaag cagtaaagct atcatggcag aatcatttgc
      481 aacaggttcc gatcatcagg ttgtaaacga gctcaacggg gaaagactga gagaacctaa
      541 cgaagttttt aaacgtttag gtcgagcaat aagatataat tttcaagtgg acgatgcaaa
      601 atttagacgc gacaatgtaa aagaaataat ttctaattta ttcgctaaca aagttgacgt
      661 tgaccatcct gaaaataaat acaaagactt taaggatctc gaagataacg ttgaaaaaag
      721 acttcaaaat cgccaggcga aatatcaaaa tgaaattaat
//
LOCUS       DAZOZD010000604          757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZOZD010000604 DAZOZD010000000
VERSION     DAZOZD010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.612"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            152..736
                     /locus_tag="ACHUPB_004865"
     CDS             152..736
                     /locus_tag="ACHUPB_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761398.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          222 a          154 c          155 g          226 t
ORIGIN      
        1 acaataggcg tcctaatgaa caatggttgt aaattttggt aacaaatatc attgctcatt
       61 aatatctagc atctgcttat aacatttgtg tcatgaatag tcattcttca aaccagtagt
      121 ctgactaatg gaattttatt caaggatttt tatgtctaaa aaattaggtt ttgccctgtc
      181 tggtcttatg ctggcaatgg ttgcaggtac tgcttctgct gatatggatg gtggtcagtt
      241 aaatatcagt ggtctggttg ttgataacac ctgtgaaacc cgcgttgacg gcggtaataa
      301 agatggtctg atcctgctgc aaactgcaac tgttggtgaa atcgatgctg gcgtgctgaa
      361 tgacaccgtt ggcgctaaag ccaaaccttt cagcatcacc gttgactgca gcaaagcaaa
      421 tccgaatcca ggtagtacag ctaaaatgac cttcggttct gttttctttg gtaatagcaa
      481 aggaacgctg aacaacgaca tgtctattaa caacccgtct gacggcgtga atatcgctct
      541 gcacaatatt gatggttcca ctatcaaaca ggttcaaatt aacaaccctg gcgatgtgta
      601 tactaaagcc ctggatgcaa cgacaaaatc tgctgtttat gattttaaag cgtcttacgt
      661 tcgtgccgtc gcagaccaaa cagcaactgc tggttatgta aaaactaaca ctgcatacac
      721 cattacttat cagtaagtat tcaccaatta aattgat
//
LOCUS       DAZOZD010000605          752 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZOZD010000605 DAZOZD010000000
VERSION     DAZOZD010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.613"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            54..>752
                     /locus_tag="ACHUPB_004866"
     CDS             54..>752
                     /locus_tag="ACHUPB_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0761399.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYH"
BASE COUNT          248 a          130 c          165 g          209 t
ORIGIN      
        1 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
       61 gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt
      121 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
      181 taaacacatc tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg
      241 ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac
      301 gttatgaagg ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg
      361 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
      421 cggcaggttc tgtcagctat atgtttgaca atgctggtgt gattgtattt aaaggatcag
      481 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
      541 aagaagaagg taacattgtt atttataccg aacctactga ccttcataaa ggaatcgcgg
      601 ctctaaaagc agctggaatc actgagttct caacaacaga attagaaatg attgctcaat
      661 ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
      721 aagatgacga cgatgtacaa aaagtttatc at
//
LOCUS       DAZOZD010000606          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZOZD010000606 DAZOZD010000000
VERSION     DAZOZD010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.614"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>750
                     /locus_tag="ACHUPB_004867"
     CDS             <1..>750
                     /locus_tag="ACHUPB_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0761400.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQH"
BASE COUNT          236 a          154 c          151 g          209 t
ORIGIN      
        1 attgccaaaa cacgtcacca aattaactta tcgaattcga cctcattttc caaagatggc
       61 tattcctcca acaatactgg aattactggc atagccggtg aacatgatca gttaaattat
      121 ggtatatatg ttaatcagca acaacaaaat aatgatacct cgcttggtac gaatttaagc
      181 tggagaactc ccatcgccat aatagatggc agctatagtc attctaaaaa cgcctggcaa
      241 agtggtggaa gtattagtag tggattagtt gtctggcccg gtggtattaa tatcactaac
      301 cagttatccg atacatttgc aattctggat gcgcctgggt tagaaggtgc acatattaat
      361 ggacaaaaat acaatcgaac aaacagcaaa ggccaggttg tttacgacct gataatacct
      421 catcgtgaaa accatctggt acttgatata gcaaacagtg aaagtgaaac agaattgcag
      481 ggcaatcgtc aaattattgc accttaccgt ggagcagttt cttatgtgca gtttacaact
      541 gaccaacgta agccctggta tatacaggca ctgcgtcccg atggttcgcc attaaccttt
      601 ggctatgacg tactggatct ccaggaaaat aatattggag tcgttggcca gggtagtcgc
      661 ctttttattc gcgtagatga aattcccact ggaataaaag ttgctctcaa tgatgaacag
      721 aacttattct gtactattac ttttcaacac
//
LOCUS       DAZOZD010000607          750 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZOZD010000607 DAZOZD010000000
VERSION     DAZOZD010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.615"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(32..151)
                     /locus_tag="ACHUPB_004868"
                     /pseudo
     CDS             complement(32..151)
                     /locus_tag="ACHUPB_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            395..750
                     /locus_tag="ACHUPB_004869"
                     /pseudo
     CDS             395..750
                     /locus_tag="ACHUPB_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611261.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          205 a          190 c          190 g          165 t
ORIGIN      
        1 cacagtacct aaagtaccgt ggagatcatc cggggaagcc ccgggtctga taccagtgca
       61 ggtagaccgc tatcttgttc cggtaatgtt ctgccgttct ttacggcatc acctctagca
      121 gaaacatcgc gtaactgcgc caagtatgat gaacttctct taccgctgcg atctggttca
      181 acaatatgca gatggtacac aacctcttta accagcccat ccggattatt cagagccgct
      241 tccgcaatcc ggaataaaag tgctgtttta ccgtgaaccc tctgaaaatc agcgagcagt
      301 gttttaacca gccgacgttc agagcgtact gacagacggt ggacaatttg aataaacagt
      361 tcaatcagac cgtagatgct ctcccgttgg cgacatgctg ggagcggtgg aacaccgctt
      421 cggcaacgag cttccggcgt ctccagtgaa gtgactgacg gataatggtt catgctaccg
      481 ggctaatgaa acacgccagt tcacccggat gttgggactt tgttgaaccg aagaacacgg
      541 cggtgcggag tccggagagt aacagaatag caaagagctt cgtgaaaaca ataaagtgtg
      601 actacatcag tatcatgccc aaacctgacg ggttaacggc agcaaagaac cttgcagagg
      661 cattcgagca ttataacgaa tagcatccgc atagtgcgct ggattatcgc tcgccacggg
      721 aatatctgcg gcagcgggcc aatgataaca
//
LOCUS       DAZOZD010000608          744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZOZD010000608 DAZOZD010000000
VERSION     DAZOZD010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.617"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            270..539
                     /gene="yacA"
                     /locus_tag="ACHUPB_004870"
     CDS             270..539
                     /gene="yacA"
                     /locus_tag="ACHUPB_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR0761401.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            539..744
                     /locus_tag="ACHUPB_004871"
                     /pseudo
     CDS             539..744
                     /locus_tag="ACHUPB_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          190 a          175 c          210 g          169 t
ORIGIN      
        1 tgactgacga ggtactggcc ctgcgattgt ctgaaaacgg ctcacaactg caccattcat
       61 aatcacatcg cataaaactg tcagcgccag tgataggaca ggtgaaacct gtcaatacgt
      121 accggcttac gccgggcttc ggcggtttta ctcctctgcc atatgcaaca acggagtgcc
      181 gccttacatg ccgctggcgc ggcatatcct ggtgacgata tctgaacagt tatatactgt
      241 gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt taatatgagt gtaagaatcg
      301 acgcagaact gaaggatgct tttatggccg ctgcaaaaag catggaccgt aatggctctc
      361 agttaatccg ggattttatg cgccagaccg ttgaacggca gcacaattct tggttccgtg
      421 accaggttgc ggcaggccgt cagcaactcg aatgcggtga tgtgcttccc catgacatgg
      481 tcgaatcctc tgctgctgca tggcgggatg aaatgagcag gaagattgct gataaatgat
      541 ggagatgttc tggaccatgc tggccatcca ggacaggaag cgcattcgta gtacattgcg
      601 gagcagaacc tgatggccgc cattgaaccg gacgaacgga ttggttattc agccagcagc
      661 ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg aaggaacatc tggaccacac
      721 aaggtagcgt gtaacgttat tttg
//
LOCUS       DAZOZD010000609          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZOZD010000609 DAZOZD010000000
VERSION     DAZOZD010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.618"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..721
                     /locus_tag="ACHUPB_004872"
     CDS             <1..721
                     /locus_tag="ACHUPB_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0761402.1"
                     /translation="NNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQHA
                     VQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDI
                     SSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHDR
                     WGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVIM
                     VTHQPGVWNLADDICDISTVL"
BASE COUNT          214 a          162 c          164 g          203 t
ORIGIN      
        1 gaacaattta agctggttta tttataaata tgacgaactt gctgaactgg ctgcggttat
       61 cgatcgcttg tatgagttcc atcaactcac tgaacagcgc cctacgaata agcctaaaaa
      121 ttgccaacat gcggtacaag tggctaatgc gagtattcgt acgcctgata ataagatcat
      181 attagagaac ttgaactttc atgtttcgcc aggcaaatgg ctattactga aaggctactc
      241 tggtgcggga aaaaccacac tgcttaaaac attatcccac tgctggccgt ggtttaaagg
      301 tgatatttct tctcctgctg acagttggta tgtgtcacaa acaccgttaa tcaaaagcgg
      361 cttactgaaa gagattattt gtaaagcact tcccctgccc gtagacgata aatcgttgag
      421 cgaagtactg catcaggttg gtctggggaa attggctgcg cgtattcatg accacgatcg
      481 ctggggagat attctttcca gcggcgaaaa acaacgtatc gccctggcac gattaatttt
      541 acgacgtccg aaatggatat ttcttgacga aactacctct catcttgagg aacaagaggc
      601 tatacgctta ctgcgtttag tgcgtgaaaa actacccaca agcggcgtca ttatggttac
      661 acatcaaccc ggtgtctgga acctggctga tgatatttgt gacattagca cggttttata
      721 acaacaataa aagccctgct gta
//
LOCUS       DAZOZD010000610          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZOZD010000610 DAZOZD010000000
VERSION     DAZOZD010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.619"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>743
                     /locus_tag="ACHUPB_004873"
     CDS             <1..>743
                     /locus_tag="ACHUPB_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0761403.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETID"
BASE COUNT          184 a          171 c          254 g          134 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag att
//
LOCUS       DAZOZD010000611          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZOZD010000611 DAZOZD010000000
VERSION     DAZOZD010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.620"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>736
                     /locus_tag="ACHUPB_004874"
     CDS             <1..>736
                     /locus_tag="ACHUPB_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR0761404.1"
                     /translation="SSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAK
                     AEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPTGK
                     ESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDK
                     YYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIEER
                     EGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT          233 a          160 c          173 g          170 t
ORIGIN      
        1 tatcttccgc tattatagtc gccatcatgg ccctcggtct gacgggttgt gatgataaaa
       61 aagccgaaac agaaacgccc ccgcctgcca atagtcaacc tgccgcacca gctcctgaag
      121 ctcccgttgc aaaagcagaa gctaaacctg aaacacctgc gcaaccggtg gtcgatgaac
      181 aagcggtttt cgacgaaaaa atggatgtct atatcaagtg ctacaacaag ttacagatcc
      241 ctgtacagaa cagtctggcg cgttatgctg actggctgaa agattttaaa caggggccta
      301 ccggtaaaga gagtactgtt tatggcatct acggcattag tgaatccaac ctcgctgagt
      361 gtgaaaaagg cgtaaaaagt gctgtggcgt taacgcctgc gctgcaacct attgatggcg
      421 tggcggtgag ttatattgat gctgccgtgg cgttgggtaa taccattaac gaaatggata
      481 aatattacac ccaggaaaat tataaagacg atgcgtttgc gaaagggaaa acgctgcacc
      541 agacattctt aaaaaatctg gaatcctttg aacctgtagc agaatcttat catgcggcga
      601 ttcaggaaat taatgataag cgccagcttg cggaactgaa aaatattgaa gaaagagaag
      661 ggaaaacatt ccactactac tctctggcag tcatgatttc agcgaaacaa attaataacc
      721 tgatttcgca agagaa
//
LOCUS       DAZOZD010000612          734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZOZD010000612 DAZOZD010000000
VERSION     DAZOZD010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.621"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>734)
                     /locus_tag="ACHUPB_004875"
     CDS             complement(<1..>734)
                     /locus_tag="ACHUPB_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISEc37 family transposase"
                     /protein_id="HFR0761405.1"
                     /translation="ICHQADVESGIFTVIHTWGRDQQWHPHIHLSTTAGGVTSGHTWK
                     NLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDTHYRRGWNV
                     NVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTKREEYLLMSG
                     DEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQITWRGMYQ
                     RLLKVDPLKCVLCGSQMRFTGLKRGYR"
BASE COUNT          162 a          234 c          158 g          180 t
ORIGIN      
        1 cggtagcccc gcttcagccc cgtaaaacgc atctgactcc cgcacagcac gcacttcagc
       61 gggtcaacct tcagtaacct ctgatacatc cctctccagg tgatttgcat cgccgttttt
      121 ctcactgtct ccgttatgat gtacaccact tcttccagta accgccgttt cgccggactc
      181 aaaaaaccgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
      241 tccatgaact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      301 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcactggt
      361 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc catcacccgg
      421 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      481 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtcagggta tttccgtgac
      541 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      601 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      661 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccga ctccacatct
      721 gcctgatggc agat
//
LOCUS       DAZOZD010000613          733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZOZD010000613 DAZOZD010000000
VERSION     DAZOZD010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.622"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..649)
                     /locus_tag="ACHUPB_004876"
     CDS             complement(<1..649)
                     /locus_tag="ACHUPB_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFR0761406.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
BASE COUNT          278 a          149 c          111 g          195 t
ORIGIN      
        1 aagcaggaac attgatatat tgttcaatca aggccttaga caggataccc cacccatcaa
       61 gcgcaacaaa taatacaagt tttattggta ctgatattgt gattggactc atcatcatca
      121 taccaagtgc aagtaaaatg cttgaaataa caagatcaac aacaacaaat ggcaaatata
      181 aataaaaacc tattttgaac gcatctttga tttcacttag cgcatatgca ggcaagaggg
      241 aaaacaatga ataatcatta ttctctgcac tctttaaatc agcattttct tcttcagacc
      301 tttggaaaaa acgtgctaac tcaagatctg tatgcttttt caagtattgc ttatattcca
      361 tcaagccaga atcagaaaaa cggacaatat cagatattgt atcaaatttt tgagggccat
      421 ttagataatt ttcataacca gcttcaataa tgggtttcat cacgaacaaa gccataatca
      481 atgcaatacc attgagagtc atatttgatg gaacctgctg caaaccaagt gcatttcgta
      541 ccattacaaa aacaatagaa aactttatat aacaggtacc agcagcaact aaaaatggta
      601 atagcgtaaa aaaagaaagt gtagcgatga gggacatgtc actcagcatg agattactcc
      661 tttaccatcc aagaactaat ctcgatacca taaccatcct caatagaaac aagttcccca
      721 tgcccaacca aag
//
LOCUS       DAZOZD010000614          732 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZOZD010000614 DAZOZD010000000
VERSION     DAZOZD010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.623"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            15..>732
                     /gene="icsP"
                     /locus_tag="ACHUPB_004877"
     CDS             15..>732
                     /gene="icsP"
                     /locus_tag="ACHUPB_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR0761407.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIF"
BASE COUNT          242 a          129 c          162 g          199 t
ORIGIN      
        1 aagtagagtt ctcgatgaaa ttaaaattcc ttgtacttgc actttgtgta ccagcgatct
       61 ttactacaca tgctaccact aactatccac ttttcatacc ggacaacatc agtacggata
      121 ttagtctagg atctctgagt ggcaaaacaa aagaacgcgt ttatcatccc aaggaagggg
      181 ggagaaaaat tagtcaactg gactggaaat acagtaatgc gactattgtt agaggtggca
      241 tcgattggaa gctaattcca aaagtgtctt tcggagtttc cggttggact actttaggta
      301 accagaaagc aagcatggtt gataaagact ggaacaattc caatacccct caggtatgga
      361 ccgaccagag ctggcatccc aatacgcatc tccgtgatgc taacgaattc gagctgaatc
      421 ttaaaggttg gttattaaat aatttggatt atcgacttgg actaatagca ggttaccaag
      481 agagtcgtta cagttttaat gcaatgggag ggagttatat ttatagtgaa aacgggggga
      541 acaggaataa aaaaggggca catcctagtg gtgaaagaac aatagattac aaacagctct
      601 tcaaaatacc ttatattgga ttaactgcta attaccgcca tgagaatttt gagtttggag
      661 cagaactgaa atatagtggt tgggttcgtt catctgatac agataaacac tatcagactg
      721 agacaatttt ta
//
LOCUS       DAZOZD010000615          731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZOZD010000615 DAZOZD010000000
VERSION     DAZOZD010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.624"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHUPB_004878"
     CDS             <1..239
                     /locus_tag="ACHUPB_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0761408.1"
                     /translation="ADRNRSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            344..535
                     /locus_tag="ACHUPB_004879"
     CDS             344..535
                     /locus_tag="ACHUPB_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0761409.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="ACHUPB_004880"
     CDS             complement(536..>731)
                     /locus_tag="ACHUPB_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0761410.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAZOZD010000616          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZOZD010000616 DAZOZD010000000
VERSION     DAZOZD010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.625"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>730)
                     /locus_tag="ACHUPB_004881"
     CDS             complement(<1..>730)
                     /locus_tag="ACHUPB_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR0761411.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAF"
BASE COUNT          194 a          194 c          155 g          187 t
ORIGIN      
        1 aaaaaggcaa acagatccga agcgggataa tctttttggg gagaattgtt ccagttagca
       61 atatatcccg acagggggtt atacacctta gggttcattt caaaaggcaa tagccctttc
      121 cagtcccatt ttcccgtacc aggaacgggt aatcgcggat catggcctga ttgacgatct
      181 ggataagcac cagtatgaac ataaccaata ttgccgttta catcagcata gtaccagttg
      241 atggtcagtg cttgtttcgc tgcctgctgt gtccactcct gccaattttt ggccttcatc
      301 tgatgagtcc aggccagcaa agacgccacc tctttaccat cccatgcgcg ggatttagcg
      361 taagccgttt gtgtcgtctg gtcagtttgg agaatgttgc catgcaccgt acgccagaca
      421 gtaaaggtct ctgcctgacc atttttcacc gtaatggttt cctcacggct taacattttc
      481 acccacttac cattatgcaa gtagtagcct ggtttctctg ccaacagccg ttcagcaaaa
      541 atatcgacat catcgccgaa acctgccgtt gatccccagg aaatcacacc attatgacca
      601 aaaaccagcc caggataggc aaatggtgta ttgccagtga catcataacc agcaccgtgc
      661 agaccaatac cataagtata cgcaggcgca taccagccaa actgcggacc atttaccatg
      721 attgctttcg
//
LOCUS       DAZOZD010000617          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZOZD010000617 DAZOZD010000000
VERSION     DAZOZD010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.626"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>730
                     /locus_tag="ACHUPB_004882"
     CDS             <1..>730
                     /locus_tag="ACHUPB_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0761412.1"
                     /translation="KVILGIILSSIYGSYGETAFAAMLDINNIWTRDYLDLAQNRGEF
                     RPGATNVQLMMKDGKIFHFPELPVPDFSAVSNKGATTSIGGAYSVTATHNGTQHHAIT
                     TQSWDQTAYKASNRVSSGDFSVHRLNKFVVETTGVTESADFSLSPEDAMKRYGVNYNG
                     KEQIIGFRAGAGTTSTILNGKQYLFGQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKN
                     SPIFGDSGSGSYLYDKEQQKWVFHG"
BASE COUNT          245 a          141 c          147 g          197 t
ORIGIN      
        1 aaagttatcc tcggtattat tttatcctca atatatggaa gttatggcga aacagcattt
       61 gcagcaatgc tggatataaa taatatatgg acccgcgatt atcttgacct tgctcaaaac
      121 agaggagagt tcagaccggg tgcaacaaat gttcaattaa tgatgaaaga tggaaagata
      181 tttcattttc cagaactacc tgtacctgat ttttctgctg tttccaacaa aggtgcaaca
      241 acatcaattg gaggtgcgta cagtgttact gcgactcata acggtacaca gcatcatgca
      301 ataacaacac agtcatggga tcagacagca tataaagcaa gtaacagagt atcatctggc
      361 gacttttcgg ttcatcgtct gaataaattc gtcgtggaaa caacaggggt tacggagagt
      421 gccgacttct cactttctcc cgaagatgcg atgaaaagat atggcgtaaa ctacaacggt
      481 aaggaacaaa taattggctt cagagcaggt gccggaacaa cctcaacgat attaaacggc
      541 aaacaatatc tgtttggaca aaactataat cccgacttgt taagcgcaag tctttttaat
      601 ctggactgga aaaacaagag ttacatttat accaacagaa ccccttttaa aaactcacca
      661 atttttggcg atagtggttc tggttcttat ctatatgata aagaacaaca aaaatgggtt
      721 ttccatggtg
//
LOCUS       DAZOZD010000618          730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZOZD010000618 DAZOZD010000000
VERSION     DAZOZD010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.627"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(162..>730)
                     /locus_tag="ACHUPB_004883"
     CDS             complement(162..>730)
                     /locus_tag="ACHUPB_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0761413.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga
//
LOCUS       DAZOZD010000619          719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZOZD010000619 DAZOZD010000000
VERSION     DAZOZD010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.628"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>719)
                     /locus_tag="ACHUPB_004884"
     CDS             complement(<1..>719)
                     /locus_tag="ACHUPB_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0761414.1"
                     /translation="FFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQC
                     GVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYI
                     HKNNKLLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTL
                     LNSMRNMDGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVC
                     HYDSETKVLTSHHGIIRNEEN"
BASE COUNT          233 a          137 c          107 g          242 t
ORIGIN      
        1 cattttcttc atttcttatt attccgtgat gactggttaa aactttcgtt tctgagtcat
       61 aatgacaaac atcgagcgtt ttaattaagt tatacgtatc accggcagag agtttataat
      121 tttctagtcg tgcagtatag ttaccattaa aattgatttc atgatttcca gccagaacga
      181 tgacattttt attaatacgg ctatctttat taccatccat atttcgcata gaatttagca
      241 aagtaaggat atatttatca ccaaaaatag tactaaatct atcgcctgta tgatccccaa
      301 ggaagataca aggggttatc gcatcgtcct ttaaaaaaac atgttgatgc aactcattca
      361 atatcatgtc tctcgtattt aataaattgt agagcaattt attattttta tgtatgtatg
      421 cagatgaagc catgttttcg tgttttaata attcacataa taaactccaa cccttttccc
      481 tgatgcctat atgccctgaa acgagcaatg cataaagtac agcgcctact cttccatctg
      541 tatcgccaaa ataagccgtg ttggcatttt gtgggttaat cacaccacat tgtttataaa
      601 agtctatttt agaaaacaca gatttaacat agctatcaaa cttatcctta ggaaacgtct
      661 ctttagagac atcccaacgc cagtcagctt taaaattacc gctaagaaga gtaaagaaa
//
LOCUS       DAZOZD010000620          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZOZD010000620 DAZOZD010000000
VERSION     DAZOZD010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.629"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..675)
                     /locus_tag="ACHUPB_004885"
     CDS             complement(<1..675)
                     /locus_tag="ACHUPB_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0761415.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSN"
BASE COUNT          217 a          152 c          129 g          207 t
ORIGIN      
        1 attgctaaag gtaattaacg tattatcaat atattcgaga gtacttatga tttcgtcagt
       61 acgctcactt cttttggcaa taaattgttc tggggtgata cctttaggat gtatggattc
      121 aaacttctga ccatcattaa tccagtcgaa aagcacctca atatcataat aaagggattt
      181 cagtagttga ttacgtgtcg cgatatgaac atcagtaata atccctccat tagtaacata
      241 gccaaataaa tcgttgcagg cagcatcaat ccaaaactca acctcgtcat tttcactaac
      301 gagagaaagg tcaaccaccc atttacctgg tttatctggc ggtacgccat aagatgatgc
      361 tttattcgtg atgccgcaga tgggtgaacc attcttatcc acaataagac cttcgccacc
      421 gatatcaacg agagcgacga gtttgtgtct aaatttatct gctggacgtt taccataaac
      481 tcgcatccag gcacagtcaa acagttgacc ccagtctgag ccaatggtga gtttctggta
      541 agcgccgcta cggcgataaa caaaaggctg cggttctttt gttatccatg cttcaatcga
      601 catttcagag acaacagtat aaacacgctg acctatttta ttcgacagtt tgataatatt
      661 atcagtaagg tacatagaaa tccctttaat taattataac actcg
//
LOCUS       DAZOZD010000621          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZOZD010000621 DAZOZD010000000
VERSION     DAZOZD010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.630"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUPB_004886"
     CDS             <1..>695
                     /locus_tag="ACHUPB_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0761416.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZOZD010000622          693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZOZD010000622 DAZOZD010000000
VERSION     DAZOZD010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.631"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUPB_004887"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUPB_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0761417.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
BASE COUNT          159 a          144 c          174 g          216 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tgg
//
LOCUS       DAZOZD010000623          689 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZOZD010000623 DAZOZD010000000
VERSION     DAZOZD010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.632"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..276)
                     /locus_tag="ACHUPB_004888"
     CDS             complement(<1..276)
                     /locus_tag="ACHUPB_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0761418.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPR"
     gene            complement(307..594)
                     /locus_tag="ACHUPB_004889"
     CDS             complement(307..594)
                     /locus_tag="ACHUPB_004889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761419.1"
                     /translation="MNRTAFRAIHAVGADTGRNCRKFPLHAAGNFRIPFVINTYKPED
                     QFFVSGIHKERNIQVCATLNGIVKIIKAQNLSFFVTGSFKPSGLFPPAPQK"
     gene            624..>689
                     /locus_tag="ACHUPB_004890"
     CDS             624..>689
                     /locus_tag="ACHUPB_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761420.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQ"
BASE COUNT          162 a          156 c          197 g          174 t
ORIGIN      
        1 ccggggacgt ttttttacca ccgggagttt gggcataacg gtgctttcag taagtacgac
       61 aggtactgtg gaggcacttc cgtaaagaag ggcatcaagt tcctgctccg acatacctgc
      121 gggcagaggc catgaaaggc cagcgttgcg gaagcgcacg aacataccac agacagttga
      181 ttttggtacg cccaggcgac gtccggccac aacccgtggt aaatgttctt cgaaatgaag
      241 gcgtaaagct tcagtgatcc aggtccggta ttccatacga tagtgtccac ttcaaaatga
      301 tggacattat ttttgtggag ccggaggaaa cagaccagac ggtttaaatg agccggttac
      361 gaagaagctg aggttctgcg ctttgataat tttgacgatg ccgttgagtg ttgcgcagac
      421 ctgaatattc cgttctttgt ggatgccgga aacaaaaaac tggtcttctg gtttgtacgt
      481 gttgatgacg aagggtatcc ggaaattgcc cgctgcatgg agcgggaatt tgcgacaatt
      541 cctgccggta tcagcgccga cggcatgtat tgccctgaat gcggtacggt tcactggccg
      601 gatggcgtta ttccgccttt ctgatgcttc cccgttttgc cgatattttt cagcagggaa
      661 accgatggct taactggctg gagaagcag
//
LOCUS       DAZOZD010000624          687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZOZD010000624 DAZOZD010000000
VERSION     DAZOZD010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.633"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHUPB_004891"
     CDS             complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHUPB_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0761421.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT          205 a          166 c          181 g          135 t
ORIGIN      
        1 gcacaataaa gccgatacag acgctaccga ggatcattgg aatgccgatt ttagagacga
       61 tatcgatccc caacgcggta gttggtgccc agacaattac cagaatcatg gtcaacgtct
      121 cacacaacat gccgccgaga atactgacgc gccagcgctg tgctttgggg attttcaggt
      181 ttatccagcc tgatatacaa cccgccagaa tgctggtgat aaagcagggg atcgccgtca
      241 cgccgccgat atcaattaaa taccggtgaa tacccgcaat cacgccggta atgatgccta
      301 cccacgggcc aaacagaatc ccgtcggaca tcaccgcgat aatacgtacg ttcaccagcg
      361 agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag aaaatggcgg
      421 taacggcgag caattctttt ggggagtgcg ccgacttgtg caacagttcg cgaaacaggc
      481 ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg aagaccgcca
      541 gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac ctctatgata
      601 ggtaacctga aggctgatga ccagcaggcc gtttttgagg aaaaaccata aatttaaaag
      661 gggattgaaa gataaatggt gtattag
//
LOCUS       DAZOZD010000625          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZOZD010000625 DAZOZD010000000
VERSION     DAZOZD010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.634"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..131
                     /locus_tag="ACHUPB_004892"
     CDS             <1..131
                     /locus_tag="ACHUPB_004892"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HFR0761422.1"
                     /translation="ISNKKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          198 a          139 c          142 g          205 t
ORIGIN      
        1 acatttctaa taaaaaaggt gatacatctc tttatgagca aagtaccaat atatctggca
       61 ccattaaaaa tagtgcaagt attgaatata ttggttttct cacttccgca ggtataaagt
      121 atattttttg aattggttca ccgagttgat tacgttttca tggctaaaaa ttgccgccca
      181 ttgtgagtca tattcgccct gactttccag aaccatacga actgcccgtt gacggacttc
      241 gggggaaaaa cgtgtatttt tagtcattct gtttacctct ttctcagggc gtttagtctc
      301 caggatttcc ggggcggttc aaaaaactct tcgttgatta ctgtggactt actgttggca
      361 ttaccgaccc tgagaccgga gaaataagaa ccgcttagat catcgtggct gttctcgggg
      421 tatcaagtta cacatgggca gaggctaccc aaggtctact attatcgcct gaaagcgctg
      481 atggaacagt gctatcaggg acatgctgat gaaaatacct aaataatcag ccagcactct
      541 atctttccaa atccacagca tagcaaagag agcaaaagag cctgtaaatt cagaaattaa
      601 ggaaacgcct cttggtgcac ataatctatc ttatgttaaa tgaaatctaa tttcttgatt
      661 atatgctgga tcgttcgtgg gtgg
//
LOCUS       DAZOZD010000626          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZOZD010000626 DAZOZD010000000
VERSION     DAZOZD010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.635"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUPB_004893"
     CDS             <1..560
                     /locus_tag="ACHUPB_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0761423.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHUPB_004894"
     CDS             complement(616..>684)
                     /locus_tag="ACHUPB_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761424.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZOZD010000627          681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZOZD010000627 DAZOZD010000000
VERSION     DAZOZD010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.636"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>681
                     /locus_tag="ACHUPB_004895"
     CDS             <1..>681
                     /locus_tag="ACHUPB_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0761425.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMC"
BASE COUNT          135 a          196 c          195 g          155 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg t
//
LOCUS       DAZOZD010000628          677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZOZD010000628 DAZOZD010000000
VERSION     DAZOZD010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.637"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHUPB_004896"
     CDS             <1..>677
                     /locus_tag="ACHUPB_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0761426.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAZOZD010000629          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZOZD010000629 DAZOZD010000000
VERSION     DAZOZD010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.638"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..621
                     /locus_tag="ACHUPB_004897"
     CDS             <1..621
                     /locus_tag="ACHUPB_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761427.1"
                     /translation="KLMTEFDDNGKIIFNINALKYTKASYFEILGEKYIKITTSSPWL
                     LEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLV
                     NFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNE
                     IDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          210 a          105 c          139 g          208 t
ORIGIN      
        1 aaattaatga ctgagtttga tgacaatggg aaaattatat ttaacattaa tgccttaaag
       61 tatacaaaag caagttactt tgaaatactt ggagagaaat atataaaaat cacaacatcc
      121 agcccctggc ttttagaaaa gctcggtaaa tatattttct cttcacgagc gcctcaagtt
      181 ctggaactcg ctattggctg gcgaggagct ttatctgaat caataaaagg agttaagttc
      241 tgtatctggt tttcagtagc ctggagaacc atagaattta ttatgagttc agagcgagat
      301 ctggttaatt ttcttggcga tttttcaatg gatgtggcca aagcagttat agccggtggt
      361 gttgcaaccg ctattggaag cctggcttct tttgcctgtg ttagctttgg ctttccagta
      421 attcttgtcg gaggagcaat tttactgaca gggatagtgt gtacagttgt tttaaatgaa
      481 atcgatgctc aatgccattt atcagaaaaa ttaaaatatg caattagaga tggactaaaa
      541 cggcaacagg aacttgataa atggaaaagg gaaaacatga ctccatttat gtatgttctt
      601 aacactccac ccgtgatatg aggattaacc atgcgtttac atgttaaatt aaaagagttt
      661 ct
//
LOCUS       DAZOZD010000630          661 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZOZD010000630 DAZOZD010000000
VERSION     DAZOZD010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.639"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..306
                     /locus_tag="ACHUPB_004898"
                     /pseudo
     CDS             1..306
                     /locus_tag="ACHUPB_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          194 a          144 c          146 g          177 t
ORIGIN      
        1 ggcgtcaaag atgtttatac gtgcgaaatt gtcggttacg ccatgggaga acgcatgacg
       61 aaagagttga cgggtaaagc cctgtttatg gcgctcagga gccagcaccc acctgccggg
      121 ctaatccacc actccgatcg aggttcacag tactgcgcat acgattaccg ggtcatacag
      181 gagcagtctg gtctgaaaac atcaatgtcg cgtaaaggta actgttacga aaacgctccg
      241 atggaaatct tctggggaac gctgaaaaat gaaagcctga gccactatcg ttttaagagc
      301 cgggatattt cgtcagcata cgggaaaact gattgaggtc ggcctcagtt ttgaggcctt
      361 tagccagttc agccgcaagg gctttaagct tcttttcgtc cataatttac ctgtctccgt
      421 tgttggagta aacatatcaa aaacaggcaa ttacacaatt taaattacag tctcaaaaca
      481 aacccaacgg tcaatatagt ttaccagcaa gcttactagc aaagtgctaa tcatttggta
      541 tctttcgtgc tattctcctt ttacgtaagt acttaagggg aacgtattta ctacttttaa
      601 agaaagctcc gttactatct gaaatgcaat gagataataa acgattactc tcggttagta
      661 t
//
LOCUS       DAZOZD010000631          660 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZOZD010000631 DAZOZD010000000
VERSION     DAZOZD010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.640"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>660
                     /locus_tag="ACHUPB_004899"
     CDS             <1..>660
                     /locus_tag="ACHUPB_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0761428.1"
                     /translation="STRESDHIANMWLSKVVDDEGKEIFSGIRHGVISAYGLKKNSSE
                     RAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKIVSTSLLTPTSLTGGEESMLKD
                     QVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVVTFNFGVNELALKMGLGWRNVD
                     KLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKNDVIYLANQIKEIINKKLQKND
                     N"
BASE COUNT          229 a           85 c          167 g          179 t
ORIGIN      
        1 cttctacgag agagagtgat catatcgcga atatgtggct ttcaaaagtg gtggatgacg
       61 aggggaagga aattttttcg ggtatcagac atggagttat atcagcctat ggattgaaaa
      121 aaaactcatc tgaaagagct gttgctgctc gtaataaagc tgaggagtta gtaagcgcag
      181 cattatatag cagaccagag ttattatcac aggctttgtc tggtaaaaca gtagatttaa
      241 agattgtttc gacttctctt ctgacgccga ccagtttaac cgggggagag gagagtatgt
      301 taaaagatca ggtgaacgca ttaaaagggt taaactctaa aaggggggag ccaacgaaat
      361 tattaattcg gaatagtgat ggtcttctga aagaagtaag cgttaatcta aaagtggtga
      421 catttaactt tggtgtaaat gaattagcgc taaaaatggg cttaggctgg aggaatgttg
      481 acaagcttaa tgatgaatca atatgttctt tgttggggga taatttcctc aaaaatggtg
      541 tgattggagg ttgggctgca gaagcaatag aaaaaaatcc accatgtaag aatgatgtga
      601 tatatctggc taaccagata aaagagatta taaacaagaa gttacaaaaa aatgataatg
//
LOCUS       DAZOZD010000632          658 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZOZD010000632 DAZOZD010000000
VERSION     DAZOZD010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.641"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>658
                     /locus_tag="ACHUPB_004900"
     CDS             <1..>658
                     /locus_tag="ACHUPB_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR0761429.1"
                     /translation="QRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCR
                     HIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACG
                     DFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVT
                     NDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLILKG
                     S"
BASE COUNT          229 a          125 c          123 g          181 t
ORIGIN      
        1 ctcaacgctc agacactctt gataaacaag aattaaacct aaaatcagat agaaaagaaa
       61 aagaattccc aagaattaaa cttaatggtc aatgctattt tccggggcga ccccaaaacc
      121 gtatagtatg ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt
      181 acaaacccca tcaagatgat tattcttcag ctgaaaaatt tctcactcac ttcaacaaaa
      241 aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg
      301 cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca
      361 tcagtgttat ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa
      421 taaagaacac aactgaagga tgtacccatt acgtggtttc ggtttatgat cctaatgtaa
      481 ctaacgacaa aataagaatc atgagcgaaa gcaaagagga tattaaacac tattctctga
      541 tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc
      601 aatctgttgc aataattcca gcacttccga aagaacagct attgatatta aaaggatc
//
LOCUS       DAZOZD010000633          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZOZD010000633 DAZOZD010000000
VERSION     DAZOZD010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.642"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>649
                     /locus_tag="ACHUPB_004901"
     CDS             <1..>649
                     /locus_tag="ACHUPB_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0761430.1"
                     /translation="LPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPL
                     TEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYL
                     LTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTI
                     TDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYK"
BASE COUNT          202 a          128 c          131 g          188 t
ORIGIN      
        1 ctgccgacgt cacagttttg gtcgtacgcg acatacttca gggtattgtc ctttgaatat
       61 ctgagtgaaa gtatttccac actgctgtat ctggatgccg atgtcgtttg taaaggaagt
      121 ctgaagcctc tcacagaaat tatatttaaa gatgagtttg ctgcggtcat tcctgacaat
      181 gttagtactc aggcggcatg tgcaaaacgc ctcaacattc ccgagatgaa tggacgttat
      241 ttcaatgcag gcgttatcta tgtcaatatt aaaaaatggc atgaagcaaa tttgacaccg
      301 tatttactca cgcttttacg aggggaaact aaatatggct ctcttaaata tttagatcag
      361 gatgcgttga atatcgcatt taatatgaat aatatctacc tcgcgaagga ttttgatact
      421 atttataccc tgaaaaacga acttcatgat cgtagtcatc gaaagtatca gcaaaccatt
      481 accgataaaa cagtattgat tcactataca gggataacta aaccatggca tagctgggct
      541 ggttatccgt ctgcatcata ctttaatatc gcgcgtgaac aatctccctg gaagaaatat
      601 cctcttaaag aggcgcggac tgttgcagaa atgcagaaac aatataagc
//
LOCUS       DAZOZD010000634          642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZOZD010000634 DAZOZD010000000
VERSION     DAZOZD010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.643"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>642
                     /gene="fliD"
                     /locus_tag="ACHUPB_004902"
     CDS             <1..>642
                     /gene="fliD"
                     /locus_tag="ACHUPB_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0761431.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          218 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac aa
//
LOCUS       DAZOZD010000635          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZOZD010000635 DAZOZD010000000
VERSION     DAZOZD010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.644"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUPB_004903"
     CDS             complement(<1..>619)
                     /gene="ipaH3"
                     /locus_tag="ACHUPB_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0761432.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          165 a          105 c          167 g          182 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgat
//
LOCUS       DAZOZD010000636          617 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZOZD010000636 DAZOZD010000000
VERSION     DAZOZD010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.645"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            58..>617
                     /gene="virB"
                     /locus_tag="ACHUPB_004904"
     CDS             58..>617
                     /gene="virB"
                     /locus_tag="ACHUPB_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR0761433.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIAS"
BASE COUNT          227 a          116 c          114 g          160 t
ORIGIN      
        1 ctcacactgc atttaacttt tgtcaatata aatcaatatg aataaacagg gtgtgatatg
       61 gtggatttgt gcaacgactt gttaagtata aaggaaggcc aaaagaaaga gtttacactc
      121 cattctggta ataaagtttc ctttatcaaa gccaagattc ctcataaaag gatccaagat
      181 ttaaccttcg tcaaccaaaa aacgaatgta cgcgatcaag aatccctaac agaagaatca
      241 ttagccgata tcataaaaac tataaagcta caacaattct tccctgtaat aggaagggag
      301 attgatggta gaattgaaat tctggatggc actcgtagaa gagcatctgc aatatatgca
      361 ggagcagatc ttgaagttct atattcaaaa gaatatatat ctactcttga tgccagaaaa
      421 ctagcaaacg atatacaaac agcaaaagag catagcatcc gagaacttgg tatcggtctt
      481 aattttctga aagtatcagg gatgtcctat aaagacatag ccaaaaaaga gaatctgtct
      541 cgcgcgaaag tcactcgtgc ctttcaggca gcaagcgttc cacaggaaat aatatctcta
      601 tttccaatcg cgtcaga
//
LOCUS       DAZOZD010000637          617 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZOZD010000637 DAZOZD010000000
VERSION     DAZOZD010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.646"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            79..>617
                     /locus_tag="ACHUPB_004905"
     CDS             79..>617
                     /locus_tag="ACHUPB_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761434.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQ"
BASE COUNT          190 a          107 c          132 g          188 t
ORIGIN      
        1 ttttttgatg tgagaaattt gtttccaaga gatacagact cttaacaacc aaaaaaaaat
       61 tttattaagg aacatattat gaaacgttca attattgctg ccgctgtctt ttcttctttt
      121 tttatgagcg ctggagtatt tgctgcagac gttgataccg gaacattaac tattaagggg
      181 aatattgcag aatctccgtg taaattcgaa gcgggtggtg attcagtaag tattaatatg
      241 ccgactgtac caaccactgt ctttgaaggt aaagctaaat attctaccta tgatgatgca
      301 gtcggtgtaa ccagcagcat gttaaaaatt agctgcccga aagaagttgc tggtgtaaaa
      361 ctctcgttga ttaccaacga taaaataacc ggtaacgata aggcgatagc cagtagcaac
      421 gataccgtgg gttactatct ctatttaggt gataacagcg atgtcctgga tgtttctgca
      481 ccttttaaca ttgagagtta taaaacagcg gaaggtcaat atgctattcc gtttaaagca
      541 aaatacctga aactgacaga taactcagtg caatcaggtg atgtgttatc ttctctggtt
      601 atgcgtgtgg cgcagga
//
LOCUS       DAZOZD010000638          611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZOZD010000638 DAZOZD010000000
VERSION     DAZOZD010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.647"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>611
                     /gene="ipaA"
                     /locus_tag="ACHUPB_004906"
     CDS             <1..>611
                     /gene="ipaA"
                     /locus_tag="ACHUPB_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR0761435.1"
                     /translation="DNRVYDNSYHENPENDAQSPTSQTNDLLSRNGNSLLNPQRALVQ
                     KVTSVLPHSISDTVQTFANNSALEKVFNHTPDNSDGIGSDLLTTSSQERSANNSLSRG
                     HRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKSSASLSHRVASQINKFNSNTDSKVLQ
                     TDFFSRNGDTYLTRETIFEASKKVTNSLSNLISLIGTKSGTQER"
BASE COUNT          225 a          144 c           92 g          150 t
ORIGIN      
        1 gacaacagag tatatgacaa tagctatcac gagaacccag aaaatgatgc acagtctcct
       61 acttctcaga caaacgatct attatcccgt aacggaaact cattactaaa tccacaaaga
      121 gcactagttc aaaaagtaac ttccgttcta ccacactcta tatcagatac tgtccagaca
      181 tttgcaaata attcagcttt agaaaaggtt ttcaaccata ctccagataa ttcggatgga
      241 ataggttcag acctgttaac tacgagtagt caagaaagat ctgcaaataa ctctctttct
      301 cggggacaca ggcctctgaa catacagaac tcttcaacca ccccccctct ccacccggaa
      361 ggagtgacaa gcagtaatga taactcatca gatacaacta aaagtagcgc ttctctttct
      421 catagagtag cttcgcaaat caataaattc aactcaaaca ctgattcaaa agtacttcag
      481 actgattttt tctcaagaaa tggagacaca tatttaacac gggaaacgat atttgaagcg
      541 tcaaaaaaag taacaaactc cctaagtaat cttatatctc tcattggaac taaatcagga
      601 acacaagaac g
//
LOCUS       DAZOZD010000639          607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZOZD010000639 DAZOZD010000000
VERSION     DAZOZD010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.648"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>607)
                     /locus_tag="ACHUPB_004907"
     CDS             complement(<1..>607)
                     /locus_tag="ACHUPB_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0761436.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILR"
BASE COUNT          197 a           99 c           85 g          226 t
ORIGIN      
        1 accttagtat taagcgtgat ttttcttgat tgcttaagct aaaatcattg acatatgcaa
       61 ccaacttatc ccaaatcaat tcctgtaatt tacggctatt tttatagggt tctttatcga
      121 gattgaaaaa taaggtctca gctattttaa ttttagattg atcgcagcta aaatctttag
      181 attgatcgca gctaaaatca atatgattta tcacggagca tgcatacttt atcttttcag
      241 aatcaccaaa atattgcatt cttttattaa aacaccaatc cagtattctt acatcattaa
      301 tatatgggga ttttaaaaaa gtctccagaa tcgaatcgat gaccggtgta aggttaactt
      361 ttgcatcatc taaggaatca ataagaatat gagacaggtt aactttgatg tcattaattt
      421 cattgtcagt agctttctta ttattaagaa gataattatt tactgcgtta aggtcatcta
      481 tttctttcag tatccctttt tcaccaagca agtatgaaat cgattcatta catacataag
      541 gcattaatag cagatgttct ttccttattg ctaataatgc attgttcaat ggtttttcta
      601 ataaatc
//
LOCUS       DAZOZD010000640          606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZOZD010000640 DAZOZD010000000
VERSION     DAZOZD010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.649"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..606
                     /locus_tag="ACHUPB_004908"
                     /pseudo
     CDS             1..606
                     /locus_tag="ACHUPB_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          200 a          115 c          114 g          177 t
ORIGIN      
        1 catcaagaaa ttaaaaaatt taaacattaa tctaaataat aacaacctga tcacataatc
       61 gggtacgatt ttatccgata actataatgc tataactctg ggaaatggtt ggaatacacc
      121 gctgggtgcc atctcttttt acgctgcccg atcgagtagt aagttaaata atgataccag
      181 acacgaaggt acgagctatc aggtcgccta taataaatat ctagtacaaa cagccactcg
      241 atttagcgtg gccgcctggc gttatgcttc gcaggattac aggacattca gcgaccatct
      301 ttacgaaaat gataaacact atcatcagag tgattatgat gatttttatg atattggcag
      361 aaaaaatagc ctttctgcca atattatgca acctttatcc aataatctgg gaaatgtatc
      421 attaagtgcg ctttggcgga attactgggg gcgaagtgga aatgctaaag attaccaatt
      481 cagttactcc aataactggc aacacattag ttatactttc tctgccagcc aatcttatga
      541 tgaaaataat aaagaagagg agcgttttaa tctgtttatc tccattcctt tctactgggg
      601 ggatga
//
LOCUS       DAZOZD010000641          604 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZOZD010000641 DAZOZD010000000
VERSION     DAZOZD010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.650"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>604
                     /gene="yagA"
                     /locus_tag="ACHUPB_004909"
     CDS             <1..>604
                     /gene="yagA"
                     /locus_tag="ACHUPB_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0761437.1"
                     /translation="LANDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSS
                     FNHEHAPECPMYRQLSGNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAY
                     HNGERSSVRKKRPRLGRLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQE
                     YIRGRALSEIIHFRPGMSERSQERLMETLENSERHWPARR"
BASE COUNT          157 a          145 c          144 g          158 t
ORIGIN      
        1 acctggccaa tgatcttctc cgggaaatgc gtgagtatga tgcatggtta cagtgtgact
       61 gtattccggg agattcgccg gccatgaatt ttgctgctct taaaaacaat acgggtactc
      121 tttatctgtc cagttttaat catgagcatg cacctgaatg ccccatgtat cgtcagttat
      181 ccggaaatga agaggaaaca tccttcggag caagccggca tcccgttagc acccgtataa
      241 attacagaaa ctttttacca cctgatgaca gtaattctgt tatcagactg caggcccgct
      301 ctgcttacca taatggtgag cgctcctccg ttcgcaagaa acgtccgcgc cttggccgtt
      361 tactcctgtc gcttattgaa gatgcagggc tgaataaact ggattccctg gctaatcccc
      421 gtatccggac taattatcgt gagtgtcttg atgccatacg gcaggttaca ctacagcagg
      481 agtatattcg gggaagagcc ttatctgaaa ttatccattt ccggccagga atgtctgaaa
      541 ggtctcagga gcggttaatg gaaacgctgg agaattcaga acgacactgg cctgccagaa
      601 gaaa
//
LOCUS       DAZOZD010000642          603 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZOZD010000642 DAZOZD010000000
VERSION     DAZOZD010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.651"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            11..603
                     /locus_tag="ACHUPB_004910"
                     /pseudo
     CDS             11..603
                     /locus_tag="ACHUPB_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
BASE COUNT          202 a          115 c          118 g          168 t
ORIGIN      
        1 ggagaattta atgatttaca ctaatcagct tcgttacagt ggcaaagatg gcatctattt
       61 ccacttaaga gacaatgtag atgccttctt aaccttatca gcaactgaaa acatggaatt
      121 tgatagccct atgcaggtga ctacacagaa catgcaatta tgaaggaagc ttattattaa
      181 ctcgtcaggg tcaattagta gaaaacttca ttgacactct ggaaaactgg cgtgaccaga
      241 agcagattat ttctgttatc tgtaaagatg gaattaaaat tgacgattcc attattacca
      301 actttaaagc ctctaaagat gttactatct ctaacggtct tcgtatccag ctaacttttc
      361 aggaaattaa ctttaaagca atcgtaggtc aaactgatat ttcagcagcc actggcaaaa
      421 cggctaccac aaatgacggt ggtgtgacca gtaagaagaa tgcaggtaac gtgaccactg
      481 aacgcaataa cgcgcctatg ctatcgtgca aagagttaat gaatattagc gatgtatccg
      541 gttacgctcc agctatcaga aatgatattc tcgatgctca gattaaatgt aataaggctt
      601 gga
//
LOCUS       DAZOZD010000643          601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZOZD010000643 DAZOZD010000000
VERSION     DAZOZD010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.652"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            324..>601
                     /locus_tag="ACHUPB_004911"
     CDS             324..>601
                     /locus_tag="ACHUPB_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFR0761438.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYA"
BASE COUNT          157 a          126 c          129 g          189 t
ORIGIN      
        1 tcacagaatt tacaaacata ctgttatcat tttgaaggca gatttggtct tatacaggca
       61 ttgctttata atctgcactc caaattctgc gggctatccg ccggtttgca gcagggaagt
      121 gtgggactgt atatgtctct tcacacggag tgttctcgat tatgtcctct aatcccagat
      181 atcacttgtt gtatcgcagt tggctatatc ctgtttctgc gcagcgcttt gggagctgaa
      241 actcaagggc ggtagcgtac ttttttgtca ggcttattct tcatttttat ttttaaccca
      301 ttgataaata atggattggt ttcatgtcaa aagcatctga accacaacag accccttatc
      361 tgatcccgca aggggtctat ccaacttata tgccaaaagc agaggatgaa atcgatcttt
      421 tcgagctttt aggcaccttg tggaagaaaa aatgggttat tttatgtgtc acgttgctga
      481 ctacggggtt agcagcagtg tatgccttta ccgcaaaaga gcagtggacg gctaaaacct
      541 atattcaagc accacgtatt gctgaattag gcagctatct taaatttcac caagcgtatg
      601 c
//
LOCUS       DAZOZD010000644          600 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZOZD010000644 DAZOZD010000000
VERSION     DAZOZD010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.653"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          185 a          140 c          119 g          156 t
ORIGIN      
        1 acggcgacgg ctcaaaattt tatcaaacct aagataattc taccatcaaa gttaataatt
       61 tgaatttata tgacggctct atgggggggt aaaaatcccc gtgcagagag gcgattttca
      121 gagttgaaaa cgtcgtcgtt aagaacacca aagtcagtac caccaacccg gaaagtagct
      181 tcatgcaccg ggacaacagc agttttggtg tccgaaatcg tacaattctg tacatctacg
      241 caaggaaatg tacggaagta agccaaatgg ttatcggacg gtcggaacat ttcgtacagc
      301 ctttacgttc agtgagttag ctggcctaga tgtaaccact cgttcaactc aattctctta
      361 gcgtgggaaa atttccgttt tccaagcggc cccctcaagc ggacccctta tagtacaata
      421 attttcgata tccaaactgc ccccaattac atctccagtc tctggcagca gagatcatat
      481 atttccattg ccgccgtagc cacgatattc tggctattca caaaaacaat ttagccccaa
      541 agcgcagtaa aaaagtacac tatgaaggtg tatgatacct tcatagtgct taagtaatta
//
LOCUS       DAZOZD010000645          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZOZD010000645 DAZOZD010000000
VERSION     DAZOZD010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.654"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUPB_004912"
     CDS             <1..91
                     /locus_tag="ACHUPB_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761439.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUPB_004913"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUPB_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0761440.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZOZD010000646          584 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZOZD010000646 DAZOZD010000000
VERSION     DAZOZD010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.655"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            100..>584
                     /locus_tag="ACHUPB_004914"
     CDS             100..>584
                     /locus_tag="ACHUPB_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0761441.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT          130 a          159 c          155 g          140 t
ORIGIN      
        1 gcatccgctt tgcaaacaag ccaatacgcg gctgaaaagg atatgcagga tgcagcgccc
       61 cgtctgactt tcactttacg agacgaagag aggttgatga tgaaaattgg cgtattcgta
      121 cctattggca acaacggctg gctcatttcg acccacgcgc cgcagtacat gccgaccttt
      181 gaactgaata aagccattgt gcagaaagcg gagcattacc atttcgattt cgccctgtcg
      241 atgatcaaac tgcgtggctt tggcggcaaa actgagttct gggatcacaa ccttgagtcg
      301 ttcaccttga tggcgggtct ggcagccgtg acctcgcgca ttcagattta cgccactgct
      361 gccaccttaa cgttacctcc ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc
      421 tctggcgggc gttttggcgt caacctcgtg actggctggc aaaagcccgt gtatgagcag
      481 atgggtatct ggcctggcga tgactatttc tcccgtcgtt acgactatct caccgaatat
      541 gttcaggtgc tgcgcgacct gtggggcacg gggaaaagcg attt
//
LOCUS       DAZOZD010000647          583 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZOZD010000647 DAZOZD010000000
VERSION     DAZOZD010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.656"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>583
                     /locus_tag="ACHUPB_004915"
     CDS             <1..>583
                     /locus_tag="ACHUPB_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR0761442.1"
                     /translation="PPQITTLEIRKNLLTHLPDLPPMLKVIHAQFNQLESLPALPETL
                     EELNAGDNKIKELPFLPENLTHLRVHNNRLHILPLLPPELKLLVVSGNRLDSIPPFPD
                     KLEGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNAFVNVAGNPLSGH
                     TMRTLQQITTGPDYSGPRIFFSMGNSATISAPEH"
BASE COUNT          189 a          136 c           98 g          160 t
ORIGIN      
        1 accgcctcag ataacaacac tggaaataag aaaaaaccta ttaacacatc tccctgattt
       61 accaccaatg cttaaggtaa tacatgctca atttaatcaa ctggaaagct tacctgcctt
      121 acccgagacg ttagaagagc ttaatgcggg tgataacaag ataaaagaat taccatttct
      181 tcctgaaaat ctaactcatt tacgggttca taataaccga ttgcatattc tgccactatt
      241 gccaccggaa ctaaaattac tggtagtttc tggaaacaga ttagacagca ttcccccctt
      301 tccagataag cttgaagggc tggctctggc taataatttt atagaacaac taccggaatt
      361 accttttagt atgaacaggg ctgtgctaat gaataataat ctgacaacac ttccggaaag
      421 tgtcctgaga ttagctcaga atgccttcgt aaatgttgca ggtaatccat tgtctggcca
      481 taccatgcgt acactacaac aaataaccac cggaccagat tattctggtc ctcgaatatt
      541 tttctctatg ggaaattctg ccacaatttc cgctccagaa cac
//
LOCUS       DAZOZD010000648          582 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZOZD010000648 DAZOZD010000000
VERSION     DAZOZD010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.657"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..574
                     /locus_tag="ACHUPB_004916"
                     /pseudo
     CDS             1..574
                     /locus_tag="ACHUPB_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5-like element ISSfl7 family transposase"
BASE COUNT          155 a          143 c          165 g          119 t
ORIGIN      
        1 acggctttat tgactggtct gccaccgcgc tggatggcag taatatccgg gcgctgaaat
       61 gtgccgccgg tgctcaaaaa aacatcccga tatcgacgga gataatgggc cgggtcgctc
      121 tcgcggcggt tttggcacca aaatccatct ggcaacagac ggaagtggcc tcccgttaaa
      181 tatcgtgcta agccccggac aagctcatga aagccagttc gcgcaacgac ttctggacgg
      241 gattggcgtt cagcgtcaga acggcagcat gaaacgccgt ggacatgcgg tactggctga
      301 taaagcgtat tcagggcgcg cattgcgcaa cgagctgaaa aataacggta taaaggcagt
      361 aatcccccga aagtcaaatg agaaaatggc atcggatgga cgtgcacagc ttgatcgtga
      421 tgcgtactgc aatcgtaatg tcgttgaacg gtgctttggg cggctgaaag aatatcgccg
      481 catcgccacg cgttacgaca aaacggcgag aaattacctg gcgatggtga aactgggctg
      541 catccgactc ttttatcaac gcttacgtaa ttaagggaca ca
//
LOCUS       DAZOZD010000649          575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZOZD010000649 DAZOZD010000000
VERSION     DAZOZD010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.658"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAZOZD010000650          574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZOZD010000650 DAZOZD010000000
VERSION     DAZOZD010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.659"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..516)
                     /locus_tag="ACHUPB_004917"
     CDS             complement(<1..516)
                     /locus_tag="ACHUPB_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0761443.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          160 a          137 c          115 g          162 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttac
//
LOCUS       DAZOZD010000651          571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZOZD010000651 DAZOZD010000000
VERSION     DAZOZD010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.660"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..429
                     /locus_tag="ACHUPB_004918"
     CDS             <1..429
                     /locus_tag="ACHUPB_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR0761444.1"
                     /translation="FSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVRIRLVNIDAPEK
                     KQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNINQFMVRAGAAWV
                     YEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
     gene            475..>571
                     /locus_tag="ACHUPB_004919"
     CDS             475..>571
                     /locus_tag="ACHUPB_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFR0761445.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSL"
BASE COUNT          166 a          117 c          148 g          140 t
ORIGIN      
        1 ttttcatggc cggtattgtc tgcagatatt catggacggg ttgttcgtgt actggacggt
       61 gataccatcg aggtcatgga ctcactgaag gcggtgcgga tcaggctggt aaatattgat
      121 gcaccggaaa agaaacagga ttatggccgg tggtcaacag acatgatgaa atcactggtc
      181 gcaggaaaaa cggttaccgt cacatatttt cagcgagatc gttatggtcg tattctcggg
      241 caggtttatg caccagacgg aatgaatatt aatcagttta tggtccgggc cggggctgcc
      301 tgggtatatg agcagtacaa cacagatccc gtgctacctg tccttcagaa cgaagccaga
      361 caacaaaaac gtgggctctg gtcagatgct gatccggttc ccccctggat atggatgcac
      421 cgtaaataaa gcgatggcaa tcctatcatt catctgttct cagcgggaat aaaaatggca
      481 aaaacaaaac aggaatggct gtatcagtta cggcgatgtt catcagtcaa tacgctggaa
      541 aaaattattc ataaaaacag agacagttta t
//
LOCUS       DAZOZD010000652          568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZOZD010000652 DAZOZD010000000
VERSION     DAZOZD010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.661"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>568
                     /locus_tag="ACHUPB_004920"
     CDS             <1..>568
                     /locus_tag="ACHUPB_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0761446.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EKKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINVLTNDKVFK"
BASE COUNT          198 a           90 c          140 g          140 t
ORIGIN      
        1 gatattaaag gaaaaatcat cacaactgat gcgatgggtt gccagaaaga tattgcagag
       61 aagatacaaa aacagggagg tgattattta tttgcggtaa aaggaaacca ggggcggcta
      121 aataaagcct ttgagaaaaa atttccgctg acagaattaa ataatccagc gcatgacagt
      181 tccgcaatga gtgaaaagag tcacggcaga gaagaaatcc gtcttcatat tgtttgcgat
      241 gtccctgatg aacttattga tttcacgttt gaatggaaag ggctgaaaaa attatgcgtg
      301 gcagtctcct ttcgctcaat aatagcagag caaaagaaag agcccgaaat gatggtcaga
      361 tattatatca gttctgctga tttaaccgct gagaagtttg cgacagcgat tcgaaatcac
      421 tggcacgtgg agaataatct gcactggcgt ctggacgtgg taatgaatga agacgactgc
      481 aaaataagaa gaggaaatgc agcagaatta ttttcaggga tacggcacat tgctattaat
      541 gttttgacga atgataaggt attcaagg
//
LOCUS       DAZOZD010000653          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZOZD010000653 DAZOZD010000000
VERSION     DAZOZD010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.662"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..131
                     /locus_tag="ACHUPB_004921"
                     /pseudo
     CDS             1..131
                     /locus_tag="ACHUPB_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6..122
                     /locus_tag="ACHUPB_004921"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(173..442)
                     /locus_tag="ACHUPB_004922"
     CDS             complement(173..442)
                     /locus_tag="ACHUPB_004922"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0761447.1"
                     /translation="MWCFFNLFGVLIPIDERNLTRERTQVGLQAARARGRKGGRPKTL
                     SKDKQALAVQLYNEKKHTVAQICVLMGISRPTLYKYIESARLFKK"
BASE COUNT          134 a          140 c          123 g          156 t
ORIGIN      
        1 gcttccgctt attttgcgaa ggcggagttc gaccgcctct ggaaaaaatg atgccactgc
       61 tggataagct gcgtgagcag tacggggtcg gaccgctatg cagcgaactg catattgccc
      121 cgtcaacgta ttagggattt gaagcccaac cgtacgaaaa cgtacgctaa gttcatttct
      181 tgaacaacct ggctgactct atgtatttgt acagcgttgg cctcgatatc cccatcaaca
      241 cacaaatctg cgcaactgta tgttttttct cgttatagag ttgaacagca agggcctgtt
      301 tatccttact cagtgttttc ggcctgccgc ccttacgtcc tctggctcgt gctgcttgaa
      361 gcccgacctg agttctctct cttgtcaggt tgcgttcatc gataggaatt aaaaccccaa
      421 aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg ctccatatga
      481 acggaaccga cgattaaact ggatggctct gattgattca gggtatgaat ggcggttttt
      541 tgctccgttt ccc
//
LOCUS       DAZOZD010000654          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZOZD010000654 DAZOZD010000000
VERSION     DAZOZD010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.663"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="ACHUPB_004923"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="ACHUPB_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR0761448.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAZOZD010000655          551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZOZD010000655 DAZOZD010000000
VERSION     DAZOZD010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.664"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..335
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004924"
                     /pseudo
     CDS             1..335
                     /gene="tnpA"
                     /locus_tag="ACHUPB_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          159 a          132 c          118 g          142 t
ORIGIN      
        1 gacgtcagat ttttgaccat gacgcgacag aaaaactacg cacttacttt tcaaatgtat
       61 gtgctgattt tgaagctgaa ctggttgaaa tggatggcga accagatcac gtccatttgt
      121 taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcgtat
      181 cgggtaggtg actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
      241 ttttgtggag tgttggctat tttgccagta gctgcggagg tgcgccaata tccgtcatcc
      301 gccaatacat tgaacaacag caaacacctg gttaggtgga aaaccacgcc ttatatccct
      361 gccctgaagg acggggtttt acggcgcacc ggataagcct ggagattatc tacaccagca
      421 tggacatcat tgtactatgc cccgtacatg ccccatcaca tcctcaacat caaagtatta
      481 gagccttgca aaccattatg cgtctcagtt tgtctcatct atcttacgca aaaaaaaata
      541 gaactaatat c
//
LOCUS       DAZOZD010000656          549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZOZD010000656 DAZOZD010000000
VERSION     DAZOZD010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.665"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>549
                     /locus_tag="ACHUPB_004925"
     CDS             <1..>549
                     /locus_tag="ACHUPB_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0761449.1"
                     /translation="VVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAP
                     LVKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFDSQT
                     GRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYKASR
                     EPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          113 a          150 c          166 g          120 t
ORIGIN      
        1 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
       61 cgaatgggtg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgccggagct
      121 gcactggccc cgctggtgaa actgctacgc catgagcttc tgacacggga tgttattcat
      181 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
      241 tggctgtgtg cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg ttttgactcg
      301 cagaccggac gggccctcag ataccctgag acctggctgc agtgctggtg tggcggtacg
      361 ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatcaccc cggcatcaca
      421 tcagcctgtt gctggagtca cgcagggcgc ggcttcgcaa atctgtataa ggccagcagg
      481 gagccccgtg caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat tgagaaactc
      541 atccgtgag
//
LOCUS       DAZOZD010000657          548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZOZD010000657 DAZOZD010000000
VERSION     DAZOZD010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.666"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            97..>548
                     /locus_tag="ACHUPB_004926"
     CDS             97..>548
                     /locus_tag="ACHUPB_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0761450.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGR"
BASE COUNT          207 a          119 c           73 g          149 t
ORIGIN      
        1 tataaactga aatttcttaa tcttgattat aacaagctag catcgctgga tgtctcccgg
       61 caagaatctc tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac
      121 aatcatccca ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacgcc
      181 aaaaacacta caaaactgga atatttaaac ttaagcaata acaatttact gccaaccaat
      241 gacattgatc aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac
      301 aatgatccac ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact
      361 aatgaagtga ccattgattt atcaggactt aatttaacca ctcaaccacc agggctgcaa
      421 aacttcacat ctatcaatct tgataataac caactcacac attttgatgc aaccaactac
      481 gatagactcg taaagctaag tctgaatagt aatgctcttg agtcaataaa ttttcctcaa
      541 ggcagaaa
//
LOCUS       DAZOZD010000658          545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZOZD010000658 DAZOZD010000000
VERSION     DAZOZD010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.667"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..291)
                     /locus_tag="ACHUPB_004927"
     CDS             complement(<1..291)
                     /locus_tag="ACHUPB_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0761451.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMER"
BASE COUNT          154 a          113 c           81 g          197 t
ORIGIN      
        1 tcgttccatc atggctttaa tttttgacgt atcaccacct gccaacttta ttgcctcgta
       61 aaaaacttca gctctgtcac gcgcaaacaa aaggcttttt cctgttaagc ttgtttggtt
      121 ctgtaaatat aatcgattaa gcgcttcgat ccccttattt ctttctccat ttttaactct
      181 ttgatatgaa atcacgctta cacctttgtg ctttccggtt tcttctgcgc ctttatagga
      241 atctccgctt tgaaatgaaa cctgatgcaa tcccaaatta gttatgtgca tttaattatc
      301 ctcagtcata aatggggcat tgattgcagt tttgtacctc gttttacaac aaaaaagata
      361 cttattcaac agggttctct ctttttctat aacactccga gcctgatata tcagacactt
      421 taacttgtgg gatttcatga tgaaacgagc actacatatg ctcacgaggt acatattatg
      481 tacaacaaaa gaaaatttga ttatgttaac aggctcaagt ttctaaattt attaattgaa
      541 tatat
//
LOCUS       DAZOZD010000659          544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZOZD010000659 DAZOZD010000000
VERSION     DAZOZD010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.668"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..503
                     /locus_tag="ACHUPB_004928"
     CDS             <1..503
                     /locus_tag="ACHUPB_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0761452.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSY
                     REVNRD"
BASE COUNT          196 a           95 c           97 g          156 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtg
//
LOCUS       DAZOZD010000660          543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZOZD010000660 DAZOZD010000000
VERSION     DAZOZD010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.669"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHUPB_004929"
     CDS             complement(<1..101)
                     /locus_tag="ACHUPB_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761453.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGM"
     gene            complement(224..>543)
                     /gene="tabA"
                     /locus_tag="ACHUPB_004930"
     CDS             complement(224..>543)
                     /gene="tabA"
                     /locus_tag="ACHUPB_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0761454.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          155 a          132 c          102 g          154 t
ORIGIN      
        1 ttcattccaa cacgccaggt atttaaaact tcactttttt gtttaaaatt gtcggctaat
       61 gtaatgtgtt gagaataatt caaatctgat attttgctca tgacgaactc cttgagttaa
      121 taagaacgtc taagcattac aaattttact cacaaaaaaa tatcaaaaaa cgctcttttc
      181 acaaactcgc cgtaaacctg gagttattta cggcgaaaag tcatcacgcc atcagcatct
      241 tcactactgc tttgcgaacc tgggctggtg cacccactgc gcacagcggt ttatgcactt
      301 cccccggata aaacacaaca aaatcacctt cattaaggat aactgttttc tcatcaacgc
      361 cttccggcaa aaatgcgatg tctttatcag ctaaccagtc ggtatccggc gtgcctgcag
      421 gttgcgtgct gaaggtcatg ccttcctgac cttttaacac aatctgaatg tcgagatagc
      481 gggcatggta ctccgtacgg cgagcttcgt gcggctcggt catatcttcc gagataagat
      541 aaa
//
LOCUS       DAZOZD010000661          541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZOZD010000661 DAZOZD010000000
VERSION     DAZOZD010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.670"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            38..>541
                     /locus_tag="ACHUPB_004931"
     CDS             38..>541
                     /locus_tag="ACHUPB_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761455.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGN"
BASE COUNT          157 a          111 c          129 g          144 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 t
//
LOCUS       DAZOZD010000662          534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZOZD010000662 DAZOZD010000000
VERSION     DAZOZD010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.671"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..335
                     /locus_tag="ACHUPB_004932"
     CDS             <1..335
                     /locus_tag="ACHUPB_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin, shufflon protein A"
                     /protein_id="HFR0761456.1"
                     /translation="TSGSLNGSYTNLGSHRGSFSGRNSGGSTLFIYASGGNGGSAGGA
                     CANTSRLQGYVGGTLISVNASNNPAYGKTAFISFAVPAGTSYQITSYPTENTSCGAGV
                     FSVFGYQT"
     gene            complement(332..>534)
                     /locus_tag="ACHUPB_004933"
     CDS             complement(332..>534)
                     /locus_tag="ACHUPB_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761457.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          136 a          119 c          126 g          153 t
ORIGIN      
        1 agacttctgg ttcgctcaat ggttcttaca caaacttagg ttcacataga ggttcattct
       61 cagggcggaa ttcagggggc agtacattgt ttatttatgc atctggaggt aatggaggat
      121 ctgccggagg tgcatgtgca aatacatccc gactgcaggg atatgttggt gggacgctaa
      181 ttagcgtgaa tgccagcaat aaccctgcat atggaaaaac agcctttatc agctttgctg
      241 tacctgcagg tacttcctat cagataacat cctatccaac agaaaataca tcatgtggtg
      301 ccggggtatt ttcagtattt ggatatcaaa cttaattgag cgttacacac gacgcaatac
      361 aacctgtctt atgctcgtat ttgtaccatt tgcgtgaacc tgctgggctc tcaactacct
      421 ggcagtagtg ggcatcctct gcgttgccca ttctggcaat ggcacagaag tcgaattgcc
      481 ccagataacc tgtggtggaa agctgagtaa ctttgagttt tccacctatt gtcc
//
LOCUS       DAZOZD010000663          532 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZOZD010000663 DAZOZD010000000
VERSION     DAZOZD010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.672"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          159 a          101 c          102 g          170 t
ORIGIN      
        1 atgtttaatt gatttgaatg ttcgtaagct atatcactta ctcaatccat tttacccaga
       61 gtcataaaat agagtgtctg ttgatctcat tttcatattc ctttcagact gaagtgcgtt
      121 ttcatcaccc gatgagaaag aactgaagtg atgtttgcca tagattgcga gatgccctgt
      181 ctcattgact atcgcggtgc acctttatac ctgttatacc agatcaaaaa tcacgcaagc
      241 catacaacaa aacgagattt gcaattcgtg tcacaaaata tgtcgatctt tttctaagag
      301 gaagatgcca tgtgaagcca gacgaacact tgaggtggtc ttcaaaaact aaaggatttc
      361 ttttaactgt ttgttttata aaaaatttat ttagggtcta aacaagggaa gctttgcaag
      421 ctaactcagt gagcgtggtg aaaacaagtg tttacccgcc atcaggctga tcataattct
      481 caccatgaaa tatgtttcct gatttgtggc ttgtaactgg tcgcttctga ag
//
LOCUS       DAZOZD010000664          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZOZD010000664 DAZOZD010000000
VERSION     DAZOZD010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.673"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            8..>531
                     /locus_tag="ACHUPB_004934"
     CDS             8..>531
                     /locus_tag="ACHUPB_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0761458.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
BASE COUNT          162 a          100 c          110 g          159 t
ORIGIN      
        1 aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt tattcgtgtt
       61 cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt ccattaatag
      121 tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt acatcaaggc
      181 gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg agattaaatc
      241 cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag ccggtagtag
      301 caaaactgtg cgctttgttg caatggaacc agagcaaaaa gaaaaaaatt atcgcgttaa
      361 atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc tctctatgca
      421 gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc aacctattgc
      481 taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac g
//
LOCUS       DAZOZD010000665          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZOZD010000665 DAZOZD010000000
VERSION     DAZOZD010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.674"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUPB_004935"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUPB_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUPB_004936"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUPB_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          171 a          104 c          109 g          147 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta g
//
LOCUS       DAZOZD010000666          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZOZD010000666 DAZOZD010000000
VERSION     DAZOZD010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.675"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(81..479)
                     /locus_tag="ACHUPB_004937"
                     /pseudo
     CDS             complement(81..479)
                     /locus_tag="ACHUPB_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          111 a          140 c          126 g          154 t
ORIGIN      
        1 tgagatgtgg agccagaaaa atggctgcgt tacgtcattg aactgtcctt gacccacgat
       61 attcagacag tcgttgtatc ttaagttgac attacccgct gacgtcgata ttcccttggt
      121 gagagataac ccaacgcact atgcggatgg ttttcattgt aatgctcgat cgccactgca
      181 aggttatgca atgccgttct tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt
      241 ttcacgaacc gttctgctat tccattactc tgagggctac tgactgccgt cgtgcagggc
      301 tccagattca actctctggc gaactgccgt gtttcatgag ccgtataagc cgaaccgtta
      361 tcggccactg gattggccgt tccggtcctt tatcgccata tcgctgacat gacatcctgc
      421 accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa
      481 tatggaacgt aacgctgtca tcgggagaat cgtcaatgtg gcattcggtt a
//
LOCUS       DAZOZD010000667          528 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZOZD010000667 DAZOZD010000000
VERSION     DAZOZD010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.676"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>528)
                     /locus_tag="ACHUPB_004938"
     CDS             complement(<1..>528)
                     /locus_tag="ACHUPB_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761459.1"
                     /translation="EISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMC
                     NLSQETILTLLAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAK
                     DSHDLPGLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKA
                     IMAYGDILKILPPHQ"
BASE COUNT          155 a          109 c           91 g          173 t
ORIGIN      
        1 cctggtgagg ggggagtatt tttagtatat ctccatacgc cataatggcc tttgtgtgcc
       61 cattttgaag tgccataaac aacccatgct ttactttcaa taattcttta atttgttcac
      121 tactaagcat tttcttatta agcagacttc cgaacatacg aatactatct gcatgtccat
      181 tctgcaatgc aaaaaataac ccaggtaaat catggctgtc tttcgcagct aatagatata
      241 atctcttatc ggaagttaac ggggttttaa agactatttc cccataggct tttattgtgt
      301 ctacatgtcc gttcataaaa gacatgcata aacccggcac attgtttgcc cctaccgcag
      361 caagcaatgt aagtattgtt tcttgactaa gattgcacat atttaatatc ttaccatata
      421 cttgaataat gtctgaacat ccgttttgaa gtgctatata aaatgcagga gtaccatcag
      481 agcttctcgt ggccagaagg ttcaccagtt tagattgaga aatttcac
//
LOCUS       DAZOZD010000668          520 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZOZD010000668 DAZOZD010000000
VERSION     DAZOZD010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.677"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>520
                     /locus_tag="ACHUPB_004939"
     CDS             <1..>520
                     /locus_tag="ACHUPB_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0761460.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAZOZD010000669          518 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZOZD010000669 DAZOZD010000000
VERSION     DAZOZD010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.678"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(188..379)
                     /locus_tag="ACHUPB_004940"
     CDS             complement(188..379)
                     /locus_tag="ACHUPB_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0761461.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            397..>518
                     /locus_tag="ACHUPB_004941"
     CDS             397..>518
                     /locus_tag="ACHUPB_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761462.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLI"
BASE COUNT          170 a           91 c          116 g          141 t
ORIGIN      
        1 aaaatatatc attacaacat atatattaat tttatattta ttccgcagcc aaacaggata
       61 aacatcagtc aggacaaaga cgtaaaattt aagatgaaag aatatctgcc gctttgataa
      121 tcccaaagag caaaagcatc cgacagcgaa gtgccgttat gttctgtgaa agtgaagaag
      181 gtaaccgcta acgttgtacc ggtgattcga cggagagtgt ggattgctga gcttcttttt
      241 tgctctgatg gtgctgccag gcaccgacgg acgagtagat aaaacggcca aagaagaaga
      301 taaagctgat gagcagtacg atacgggtca tgcgactgtt aaatcggtgt cgttttcgca
      361 tactggttgc ctgactcaca aaaggttcct tgaagtatgt cccacgctgt ggacggtact
      421 tacattaagg cacaacagga caaaatggtc aattcttcgt tatgtaaaaa aacgtcagtg
      481 gatacatatt ttaatgttat ggaagttaat ttaattat
//
LOCUS       DAZOZD010000670          515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZOZD010000670 DAZOZD010000000
VERSION     DAZOZD010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.679"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          159 a           85 c          116 g          155 t
ORIGIN      
        1 aatgtgtaga ggataactga gtttggtcac tttaactgta ttagtcgcag tctgactctc
       61 gactttaaaa ggatgggaac tacctgtact gatagtgaga aagctttcca ttgaaataag
      121 tcaaataaaa gttgatggct tcagtgctaa agtgaggtgc ccgctagccg tattcactcg
      181 aatcggaatt caatgtattg cttctgccat ttccataata tttcacggga aaaacaaaat
      241 ctgaaaaact atacttaagt gttgtattga gtcagttgag atacaaatac atatttatca
      301 gcacacgtta cgtcaaatac aggaggttgt catgttggta ctgaaaggca cgttggctat
      361 tgcagccgtg atgacaattt attgtcttat cgttgttctt atggaacgct tttctgattg
      421 atttcatatt ggcgagagaa caagaaggtg agtagaattg tcgcggaatc ctgaaactat
      481 cagcctcagg tatgaacacc aaaaggtagg tagag
//
LOCUS       DAZOZD010000671          514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZOZD010000671 DAZOZD010000000
VERSION     DAZOZD010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.680"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUPB_004942"
     CDS             <1..173
                     /locus_tag="ACHUPB_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0761463.1"
                     /translation="VIHRKSWKNRAEVELAILTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     gene            215..415
                     /locus_tag="ACHUPB_004943"
                     /pseudo
     CDS             215..415
                     /locus_tag="ACHUPB_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          159 a          117 c          126 g          112 t
ORIGIN      
        1 aggtaataca ccgtaagagc tggaaaaacc gtgcagaagt ggaactggcc atactcacgt
       61 gggtggactg gtataacaat cgacgattgc tggaaaggct gggccatact cctccggcag
      121 aagcagaaaa agcttattat gcttccatcg gaaacgatga tctggcagcc tgagttcaca
      181 gataaaacac tctccaggaa acccggggcg gttcaacctt cacgataaaa aacttttcat
      241 taaaggtaaa gcggatcaat atccggcact tcattctctg atatctccga cccgcattaa
      301 tctgaaagaa atagaaattc actggcgtga agtgctacgt ctggcgacct caataaaaca
      361 gggaacagtg acagcatccc tgatgctgag aaaacttgcc agttacccca aacaaaagta
      421 aggcagtatg actttccgtg agacagatgc tggccaacgt gggatattta acgcgggtta
      481 acatcgttgt cagagccgca tgggtgagct gttg
//
LOCUS       DAZOZD010000672          513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZOZD010000672 DAZOZD010000000
VERSION     DAZOZD010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.681"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>513)
                     /locus_tag="ACHUPB_004944"
     CDS             complement(<1..>513)
                     /locus_tag="ACHUPB_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0761464.1"
                     /translation="LVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGSVPR
                     SIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGNPLS
                     PYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAMLKGD
                     DVYINGDGETS"
BASE COUNT          166 a          102 c           89 g          156 t
ORIGIN      
        1 actcgtttca ccatcgccat taatatatac gtcatcacct ttaagcattg ctgctgtcca
       61 ttttggaatt actgcagcat aagctccatt aggatcttga cgacgaccaa atacattaaa
      121 ataacgtaat ccaatagttt taaaaccata tgttcgagca tatacctgag cataaatctc
      181 gttaacatat ttagtaactg cataaggaga aagtggatta ccaatgtttt cctctacttt
      241 tggtagtgcg ggatgatctc cataagttga gcttgatgca gcataagtaa aactttgtac
      301 ttgtgcattt ttagccgcat gtaagatatt caaaaatcca gtaatattag ttgcattggt
      361 tgttatagga tcaacaattg aacgaggtac agaacctagc gcagcctgat gtaagacatg
      421 atcaacacct ttcataactt gctcacaggt agtgagatct cgaatatcac cttctataaa
      481 gcaaaatcga ctccactgtt cagtggaaac taa
//
LOCUS       DAZOZD010000673          513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZOZD010000673 DAZOZD010000000
VERSION     DAZOZD010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.682"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            50..217
                     /locus_tag="ACHUPB_004945"
     CDS             50..217
                     /locus_tag="ACHUPB_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0761465.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT          149 a          113 c           86 g          165 t
ORIGIN      
        1 tcatataatc aatgaatatt aattaatata taatacatag ggaatgtaaa tgaacaaatt
       61 tattaaagtt gcactggtag gtgcagtact ggctacgtta actgcatgta ctggtcatat
      121 tgaaaaccgt gataagaact gctcttacga ctacctgctg cacccggcaa tttctatttc
      181 taaaatcatt ggcggttgcg gtcctactgc acagtaagtc tctgacagaa atcggctaac
      241 accggtttaa ttgcccgacg ccctctggat tttccgaggg cgtatttgtt tctatgtcct
      301 tcttgccccc gcccgtaaaa atattttcac tttaaattca atttgataac tacatccatt
      361 agccacagcg cgatcctgtg cgaaattttg tgatcttcct ccacattaca taacatcata
      421 tgttgttata ttcatcatgc attgtcatgt taccttttaa atgactgcaa actctcccct
      481 acaacgtatt ggacaagaaa aaggtatcgc tat
//
LOCUS       DAZOZD010000674          512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZOZD010000674 DAZOZD010000000
VERSION     DAZOZD010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.683"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>512)
                     /gene="espL1"
                     /locus_tag="ACHUPB_004946"
     CDS             complement(<1..>512)
                     /gene="espL1"
                     /locus_tag="ACHUPB_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR0761466.1"
                     /translation="ALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPP
                     AGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYV
                     VSVYDPNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEG
                     VPVAILAGITT"
BASE COUNT          149 a          116 c          119 g          128 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctcaa tgaatcaagg gc
//
LOCUS       DAZOZD010000675          509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZOZD010000675 DAZOZD010000000
VERSION     DAZOZD010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.684"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..448
                     /gene="ipgC"
                     /locus_tag="ACHUPB_004947"
     CDS             <1..448
                     /gene="ipgC"
                     /locus_tag="ACHUPB_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR0761467.1"
                     /translation="NESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAYDFYNKGR
                     IEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKNDYTPVFH
                     TGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
BASE COUNT          178 a           94 c           90 g          147 t
ORIGIN      
        1 aaatgaaagc atctctactg cagtaattga tgcaattaac tctggcgcta cactgaaaga
       61 tattaatgca attcctgatg atatgatgga tgacatttat tcatatgctt atgactttta
      121 caacaaagga agaatagagg aagctgaagt tttcttcaga tttttatgta tatacgactt
      181 ttacaatgta gactacatta tgggactcgc agctatttat cagataaaag aacagttcca
      241 acaagcagca gacctttatg ctgtcgcttt tgcattagga aaaaatgact atacaccggt
      301 attccatact ggacaatgcc agcttcggtt gaaagccccc ttgaaagcca aagagtgctt
      361 cgaactcgta attcaacaca gcaatgatga aaaattaaaa ataaaagcac aatcatactt
      421 ggacgcaatt caggatatca aggagtaatt attatgcata atgtaagtac cacaaccact
      481 ggtttgtctc ttgccaaaat attggcttc
//
LOCUS       DAZOZD010000676          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZOZD010000676 DAZOZD010000000
VERSION     DAZOZD010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.685"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUPB_004948"
     CDS             complement(<1..188)
                     /locus_tag="ACHUPB_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0761468.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="ACHUPB_004949"
                     /pseudo
     CDS             242..445
                     /locus_tag="ACHUPB_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAZOZD010000677          507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZOZD010000677 DAZOZD010000000
VERSION     DAZOZD010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.686"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(1..177)
                     /locus_tag="ACHUPB_004950"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="ACHUPB_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            161..419
                     /locus_tag="ACHUPB_004951"
                     /pseudo
     CDS             161..419
                     /locus_tag="ACHUPB_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          126 a          136 c          115 g          130 t
ORIGIN      
        1 gctgtgctgc cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg
       61 cggatgttca ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa
      121 gcgatcgaag gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt
      181 gctgagccag tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt
      241 tattgtcctc aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata
      301 atctgaacct gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt
      361 ctgatgatat gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa
      421 tctgcgtcac gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg
      481 cgaacctctc gactcttgtt ggatttt
//
LOCUS       DAZOZD010000678          504 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZOZD010000678 DAZOZD010000000
VERSION     DAZOZD010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.687"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHUPB_004952"
     CDS             <1..194
                     /locus_tag="ACHUPB_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761469.1"
                     /translation="LKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKIV
                     VKYNNWITKDNGEFELAVH"
     gene            224..>504
                     /locus_tag="ACHUPB_004953"
     CDS             224..>504
                     /locus_tag="ACHUPB_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0761470.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DAZOZD010000679          501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZOZD010000679 DAZOZD010000000
VERSION     DAZOZD010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.688"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..435)
                     /locus_tag="ACHUPB_004954"
     CDS             complement(<1..435)
                     /locus_tag="ACHUPB_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0761471.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFD"
BASE COUNT          151 a           97 c           91 g          162 t
ORIGIN      
        1 gtcaaagatt ttgactgact gagtaaaaaa ttttgactca gtgggtaaag aattttgact
       61 cagtgggtca tctttcttga ctgactgagt aactttagac tccagctttg cctttctggc
      121 ttccttagct gtaagctctg ttaaaacctc cggtacttca tcaagtgaat aaactttaat
      181 aacacactga ttgcttgcaa cgttagtttt cttgattaaa ccatgtgcct caagttgttt
      241 catacatgca atcacggatg ttcttgtgat tccagcatac tgaccaagat gagcatatga
      301 aggggtataa tattgcccct tatcgtgata aaacttttgt tcattcaaca tgacagtgta
      361 aataatacgg tcggcagctt taacgccatt agtcatctga taaatacggt catcaaacat
      421 gtggaatttt tccattatat tttccttttt aaaaataaaa caaagaaagg gctgaccacg
      481 tatagtgacc aaccccagag g
//
LOCUS       DAZOZD010000680          496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZOZD010000680 DAZOZD010000000
VERSION     DAZOZD010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.689"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..159
                     /locus_tag="ACHUPB_004955"
     CDS             <1..159
                     /locus_tag="ACHUPB_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0761472.1"
                     /translation="RDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIR
                     LFYQRLRN"
     gene            298..453
                     /locus_tag="ACHUPB_004956"
                     /pseudo
     CDS             298..453
                     /locus_tag="ACHUPB_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          142 a          103 c          120 g          131 t
ORIGIN      
        1 cgtgatgcgt actgcaatcg taatgtcgtt gaacggtgct ttgggcggct gaaagaatat
       61 cgccgcatcg ccacgcgtta cgacaaaacg gcgagaaatt acctggcgat ggtgaaactg
      121 ggctgcatcc gactctttta tcaacgctta cgtaattaag ggacacagcc tggcactctg
      181 cgagtaaact tgctggtaaa ctattattga cagttggttt tgttttgaga ctgtaattta
      241 aattgtgtaa ttgcctgttt ttgatatgtt cactccaaca acggagacag gtaaattatg
      301 gacgaaaaga agcttaaagc ccttgcggct gaactggcta aaggcctcaa aaccgaggcc
      361 gacctcaatc agttttcccg tatgcagacg aaattaactg tcgaaacggt gcttaatgcg
      421 gaactaactg accatctcgg gcatgaaaaa agctacatcc ggtagtaaac attatcagtg
      481 ggttatgcga tatgat
//
LOCUS       DAZOZD010000681          493 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZOZD010000681 DAZOZD010000000
VERSION     DAZOZD010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.690"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..127
                     /locus_tag="ACHUPB_004957"
     CDS             <1..127
                     /locus_tag="ACHUPB_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0761473.1"
                     /translation="CYTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVENGQHQ"
     gene            241..493
                     /locus_tag="ACHUPB_004958"
                     /pseudo
     CDS             241..493
                     /locus_tag="ACHUPB_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          167 a           98 c          121 g          107 t
ORIGIN      
        1 ctgctacacg ctgaatatca aaggagagag ttaccggtta aaagagaaac gtaaagctgg
       61 agtgctgacc aaaaacacaa cgccaatcag tgatgatgaa atggtggaaa acggacagca
      121 tcagtaacga aagtatctta gcaggcatga aaatcgtccg catcccctaa acggtcaaaa
      181 tgcttctgcc gtgaaggagg gagaatgcca tctcatcact tatatctggc gaatgaaaca
      241 cagatggtcg gccttgcccc caaacacacg gcggtgcaaa atggaatggc agaaaacttc
      301 gtcaaaacga tgaaatatga ttaaatcagt gttatgttcg aacctgacgt gttaacagca
      361 gtaaagtagt ctcgcgaagg tgttcaaaca ttataatgaa tggcatcctc atagtgcgca
      421 gggttatcgc ttgctgcgga aatatctgaa gcgacgtacc agtaatgggg aaaatgatag
      481 ctggtttctg aaa
//
LOCUS       DAZOZD010000682          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZOZD010000682 DAZOZD010000000
VERSION     DAZOZD010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.691"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..209
                     /locus_tag="ACHUPB_004959"
     CDS             <1..209
                     /locus_tag="ACHUPB_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0761474.1"
                     /translation="AFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLN
                     SWKIAPNERGDKKGVPGLSCFQPQ"
BASE COUNT          161 a           76 c           97 g          155 t
ORIGIN      
        1 ctgctttcag accaaaacct gaagatatct tcaaatgtgg acagacgctc aaaaaaacta
       61 aacagaaata tggccattat ggattagggg tagatcagca tcaaaaaatt gataattata
      121 ccatggggct aaattcctgg aagatagcac ctaacgaacg tggtgataaa aaaggtgtcc
      181 cagggcttag ttgttttcaa cctcaataaa acttcatgag tgctgtggta tctcttttta
      241 attttgccag tatttcatag gttttcttaa tcgattgtag aggcatgttc tgatgacgcc
      301 tctacaatct ttagggactt tctctgctat gagataagaa atttcatatt atgctttaag
      361 gctgaatgat ttgtgcaaaa tataattatt ttcccatcga acatgaggtg tatgtcataa
      421 gattaaatgt taactatgta ttcctcgcgg taaataatat gaggtaaaag atgaaaattg
      481 gaactgtgg
//
LOCUS       DAZOZD010000683          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZOZD010000683 DAZOZD010000000
VERSION     DAZOZD010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.692"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="spa29"
                     /locus_tag="ACHUPB_004960"
                     /gene_synonym="spaR"
     CDS             complement(<1..>489)
                     /gene="spa29"
                     /locus_tag="ACHUPB_004960"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR0761475.1"
                     /translation="FLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLP
                     FWIFHAVGSIIDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILES
                     IQLSYNICPLFSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAP
                     QM"
BASE COUNT          207 a           91 c           83 g          108 t
ORIGIN      
        1 ttcatctgcg gagcaaatct cgataataca ccaagtaata cttctgatag taacaatact
       61 atcataacag gactggctaa aataacagcc tgacttgcca ataaagtcag aaatgttaag
      121 atatttgaga cacggaaaga acattgagaa aataacgggc atatattata agacaattgt
      181 atggattcta aaataaagac cataccacca ctgtatagaa atacaactgc agaaaaaaga
      241 ttgaaaaatt ttgccaactc agacgtatca acaccattgg caggatcaat tgaactacta
      301 agcgttgccc cacgctggtt gtcaataatg ctaccaacag catgaaatat ccaaaaggga
      361 agagacaagc aaaaagagag aaggaggcca acaattattt ccttgaacat aaggaaataa
      421 acatgttcaa aaacagaaga acctatgtct accttaccag aagtaattaa tcctgatgca
      481 acaagaaaa
//
LOCUS       DAZOZD010000684          482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZOZD010000684 DAZOZD010000000
VERSION     DAZOZD010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.693"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..143)
                     /locus_tag="ACHUPB_004961"
     CDS             complement(<1..143)
                     /locus_tag="ACHUPB_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR0761476.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVP"
     gene            complement(155..>482)
                     /locus_tag="ACHUPB_004962"
     CDS             complement(155..>482)
                     /locus_tag="ACHUPB_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761477.1"
                     /translation="NNMTIYWNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDK
                     ENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMK
                     ITGTPE"
BASE COUNT          150 a          120 c           66 g          146 t
ORIGIN      
        1 ggaactaacg tggtaacata cgatggaaga ttaattatct ctatcgtttt cacgctatct
       61 attccacaca attcattcac tcgccccatc cccaataccg aaccagaaaa tagagacaat
      121 aaaaccatca aatatttcat cataaaaaac ctcactattc tggcgtaccc gttattttca
      181 tcgtctgcaa cggtccatcc acccgataaa aaggcagtcc actatgagct cctctggtac
      241 actcacctaa agttttcgcc gcacgcgatt caattattgt aataaccctt gcctcttctt
      301 tattctcata tttcttatct gttcggctta aaagagccat gaaattttcc ttatcaagaa
      361 ggcaatactg cgcatgagta aactcatgtc ccaaacctaa tgcaggcgat tgtgatttcg
      421 agtttaattc agttgaacaa aataaagatg ctctactatt ccaatatatc gtcatattat
      481 tc
//
LOCUS       DAZOZD010000685          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZOZD010000685 DAZOZD010000000
VERSION     DAZOZD010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.694"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>480)
                     /locus_tag="ACHUPB_004963"
     CDS             complement(<1..>480)
                     /locus_tag="ACHUPB_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR0761478.1"
                     /translation="ASLTQLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLK
                     ELHVSNNQLCSLPVLPELLETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHN
                     IHSIWAIDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH"
BASE COUNT          147 a           72 c          115 g          146 t
ORIGIN      
        1 catgggatga caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg
      121 gcaggtatgg aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag
      181 gtagaggggg gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg
      241 gtaaaggtgg taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg
      301 gtaaaacagg aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg
      361 ggagtgtggg tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag
      421 gcaaagcagg cagttcaata agtctgttat agctgacatt aagctgtgta agggacgcag
//
LOCUS       DAZOZD010000686          479 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZOZD010000686 DAZOZD010000000
VERSION     DAZOZD010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.695"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..478)
                     /gene="mxiE"
                     /locus_tag="ACHUPB_004964"
     CDS             complement(<1..478)
                     /gene="mxiE"
                     /locus_tag="ACHUPB_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR0761479.1"
                     /translation="MNYHYRFNINSCAKFLAFWDYFSGALVEHSHAEKCIHFYHENDL
                     RDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLVLYLLRTIEKEKEVRIKS
                     LTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVFLHNQTITSAAMNNGYA"
BASE COUNT          161 a          101 c           67 g          150 t
ORIGIN      
        1 atgcataccc attattcatg gcagcagaag tgattgtttg attgtgcaaa aatacatcta
       61 ataaaccatt tactaaccgc caagtgttca gttgttcttt gacttttgcc cctaaagctt
      121 ttctacataa agatctaaaa taagcctccg acacaccata atgctctgtc agactcttta
      181 ttcttacctc cttttctttc tcaatagttc tcagaagata aagaaccaaa tgatatgact
      241 cagtcattcg tatcatagcc agtgcattac ttacattctg gtcagatgaa aagatgaatc
      301 ttaacattaa cttatcaagc atcgattcgg tattacaact atcccgaaga tcattctcat
      361 gatagaaatg aatacatttt tcagcatggc tatgctctac tagagcacca ctgaaatagt
      421 cccaaaaagc aagaaatttt gcacaactat taatgttaaa gcgataatga tagttcaca
//
LOCUS       DAZOZD010000687          478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZOZD010000687 DAZOZD010000000
VERSION     DAZOZD010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.696"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..140
                     /gene="ospE2"
                     /locus_tag="ACHUPB_004965"
     CDS             <1..140
                     /gene="ospE2"
                     /locus_tag="ACHUPB_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR0761480.1"
                     /translation="AIYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSN
                     I"
BASE COUNT          168 a           88 c           85 g          137 t
ORIGIN      
        1 cagctatata cgaattacaa atacctgctg ccaaccggac gaaaacgttg aaattcactg
       61 ctacagaaat gcaatggttg acaaagataa atgaagtagg aattgttgaa aaacaaagcc
      121 aacggcattc taatatctga agttaatttc agactcgttt aagctccatt ttcaaaagaa
      181 tatatcaggg tttgttacta gtaatttctg gaaacagaaa aagtagagca gtaattctat
      241 ttcagagagg agtaactcca tctatcctcg atacatactg aacgttatcg tgatatattc
      301 atcatgcttt cttcaccatt attcgagtgc agggacgcaa tgatcttaaa taccttttag
      361 tagaatatat tgctgaccca caaatatcag gatggtattg acaagtcata gatggagaaa
      421 tatatgactg aattaccatt ctctggaata acagcagact ccttcccata gacggctt
//
LOCUS       DAZOZD010000688          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZOZD010000688 DAZOZD010000000
VERSION     DAZOZD010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.697"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>476)
                     /locus_tag="ACHUPB_004966"
     CDS             complement(<1..>476)
                     /locus_tag="ACHUPB_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0761481.1"
                     /translation="VVYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGM
                     SWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPI
                     AQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          163 a          108 c           99 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag acaacg
//
LOCUS       DAZOZD010000689          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZOZD010000689 DAZOZD010000000
VERSION     DAZOZD010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.698"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHUPB_004967"
     rRNA            <1..>476
                     /locus_tag="ACHUPB_004967"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZOZD010000690          475 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZOZD010000690 DAZOZD010000000
VERSION     DAZOZD010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.699"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(246..>475)
                     /locus_tag="ACHUPB_004968"
     CDS             complement(246..>475)
                     /locus_tag="ACHUPB_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761482.1"
                     /translation="WFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVMLIKRLASS
                     LKAVKPDSCVVREAQDWLRDRKLVDITDILR"
BASE COUNT          107 a          159 c          121 g           88 t
ORIGIN      
        1 cttaccttgc atcccgcccg cagaagcccg gctacgatgg aggcacggca cagcccgcac
       61 acgacacggc ggccacataa ctgaaaaccc acaaggccat gccccgcatg gccgggtaaa
      121 agcggagccg gcggcgacgt ccttattccg gcgagagcag ggagcacacc gcatgatctc
      181 cagaccaccc ctggaacgaa acgatcatga ccggcatgtc cctgcgtaaa atatacatgc
      241 ctggttcacc ggagaatatc tgtgatatcc acaagttttc tgtcgcgcag ccagtcctgc
      301 gcttcacgca ccacacaact gtccggtttc accgctttca gcgatgatgc cagtcgcttg
      361 atcaacatca ccagttcccg tgtctgtatg tccggcattg tctccggcgg agcaggggga
      421 ctctcatttc cgacgctcat cccatgcgct tccagtgtgg cacggacaaa ccagg
//
LOCUS       DAZOZD010000691          473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZOZD010000691 DAZOZD010000000
VERSION     DAZOZD010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.700"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>473
                     /locus_tag="ACHUPB_004969"
     CDS             <1..>473
                     /locus_tag="ACHUPB_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0761483.1"
                     /translation="FLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMK
                     FVMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKD
                     ESSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
BASE COUNT          159 a           76 c          100 g          138 t
ORIGIN      
        1 atttttggta aaaatattta ctctaggtta tgtggatttt agcaaacgct ccaacgaagc
       61 gggaagaaat atgatggctc atattgagtc ctcatcttat atcaaaaata atgatggcag
      121 tgagataatg aagtttgtta tgaataatcc tgaaggggaa cgagcggatt tatctaaggt
      181 ggaaatagaa atcacacttt ccgctttcac tactatgggg actcgtcaag gacatacggc
      241 tattatattt caacaacctg atggttcgac taatcgttat gaaggaaagt cctttgaaag
      301 aaaagatgag agttcattac acctgattac taacaagatt ctggcatgtt accaacgcga
      361 agctaacaag gaaatagctc gtctattaaa tattccgcag gagttaaata attctcagga
      421 cttgaataac tcgcaggtga gttgtaaaga ttcagttgat tctacgatta cgg
//
LOCUS       DAZOZD010000692          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZOZD010000692 DAZOZD010000000
VERSION     DAZOZD010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.701"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHUPB_004970"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHUPB_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZOZD010000693          471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZOZD010000693 DAZOZD010000000
VERSION     DAZOZD010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.702"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..286
                     /gene="traL"
                     /locus_tag="ACHUPB_004971"
     CDS             <1..286
                     /gene="traL"
                     /locus_tag="ACHUPB_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR0761484.1"
                     /translation="KYRFPETLTNQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGA
                     AVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
     gene            308..>471
                     /locus_tag="ACHUPB_004972"
     CDS             308..>471
                     /locus_tag="ACHUPB_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFR0761485.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGF"
BASE COUNT          113 a          106 c          115 g          137 t
ORIGIN      
        1 gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc tggtttggcc tgccactgga
       61 tgaactgatc cccgcagcaa tctgtgttgg ctggggtatc acaacatcga aatatctgtt
      121 cggtattggt gcagcggttc tggtttattt cgggattaaa aaactgaaaa aagggcgggg
      181 cagttcctgg ttacgtgacc tgatttactg gtatatgcca acagccctgc tgcgcggtat
      241 ttttcataat gttcccgatt cgtgtttccg gcagtggatt aaatagaact gataccagga
      301 ttgttatatg gaacacggtg cccgtttaag taccagtcgt gtaatggcca tcgcctttat
      361 atttatgtca gtgcttattg ttctcagcct ctctgttaac ctcattcagg gggtgaataa
      421 ctaccgtctt cagaatgagc aacgcactgc cgtgacccca atgggattta a
//
LOCUS       DAZOZD010000694          465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZOZD010000694 DAZOZD010000000
VERSION     DAZOZD010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.703"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          145 a           83 c           79 g          158 t
ORIGIN      
        1 cttttactaa ctaacattgt ctattcctca gttaatgtct acatggctat ttttaatttt
       61 attattgttt gtcactataa aaaatcgctc atttgagaca attgctggca ttaacagctt
      121 catatgctat acatggtact tttaattaaa ttagcacaag aatgttaaat ttaataaaca
      181 aaaagttatt tcgctgtatg ataaaaacca cccgttataa tttattagtg aaattcgttt
      241 ttcgagtgtt agaaatttat atctaaatag cgttgattaa tgagcaaagc cagactcctg
      301 taacgctcac aagactcatc tacctgcgac gggtattctg gtcagtagta gatgtttaag
      361 gcgtggcaga gacatttcat ccttactcta cggcattggc ccgcatacaa atcgtggttg
      421 tgacactcac ttatcatcag tgagcgaaca gagaatagtt cagtg
//
LOCUS       DAZOZD010000695          464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZOZD010000695 DAZOZD010000000
VERSION     DAZOZD010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.704"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>464
                     /locus_tag="ACHUPB_004973"
     CDS             <1..>464
                     /locus_tag="ACHUPB_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0761486.1"
                     /translation="HIFLITGFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWS
                     EVNEMLQSGLVEFHVHTHTHTRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKH
                     LCWPEGYYNKDYIQVAEELGFYYLYTTERRMNAPAKGTTRIGRISTKERESC"
BASE COUNT          154 a           74 c          110 g          126 t
ORIGIN      
        1 acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact ctcctggaaa
       61 ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg ataatgttat
      121 gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat ttcatgttca
      181 tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac agtgtaaaca
      241 tcttcggcag gacttgttga gcgggagaga atatttgaaa gaaatgacag gaaaatgcag
      301 taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg tcgctgaaga
      361 acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg ctaaaggaac
      421 tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcg
//
LOCUS       DAZOZD010000696          463 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZOZD010000696 DAZOZD010000000
VERSION     DAZOZD010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.705"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..438
                     /locus_tag="ACHUPB_004974"
     CDS             <1..438
                     /locus_tag="ACHUPB_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0761487.1"
                     /translation="TDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLILHAPSFVSVS
                     EKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHKIKLTEAIRKL
                     RRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          160 a           75 c           86 g          142 t
ORIGIN      
        1 actgataata aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg
       61 ctttcatgta ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt
      121 gtttctgtgt ctgaaaaaat cgccagaatt gtgatggcaa attactcccg taactggagt
      181 aattccgagt tggccagtgc ggttttaatg agtgaatctt ctttaaaaag aagaatgtac
      241 aaagaggtcg gttctataag tacatttgta cataaaataa agcttacaga agctatcagg
      301 aaattacgaa ggacaaatac tcccatttct gtcatatcat ctgagttagg ttacagttca
      361 ccatcgtatt tttctaaagt tttctttaaa tatttaaaga cataccctca aaacataagg
      421 aagaaaaatg gtcggtaatg tcaccaatta ggaggacttg cag
//
LOCUS       DAZOZD010000697          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZOZD010000697 DAZOZD010000000
VERSION     DAZOZD010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.706"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHUPB_004975"
     CDS             <1..>461
                     /locus_tag="ACHUPB_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0761488.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAZOZD010000698          457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZOZD010000698 DAZOZD010000000
VERSION     DAZOZD010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.707"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHUPB_004976"
     CDS             <1..386
                     /locus_tag="ACHUPB_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0761489.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          123 g          105 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcactg
//
LOCUS       DAZOZD010000699          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZOZD010000699 DAZOZD010000000
VERSION     DAZOZD010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.708"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            16..>455
                     /gene="ygeH"
                     /locus_tag="ACHUPB_004977"
     CDS             16..>455
                     /gene="ygeH"
                     /locus_tag="ACHUPB_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR0761490.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIIS"
BASE COUNT          155 a           72 c           81 g          147 t
ORIGIN      
        1 catagaggtt aactaatgga cttagaaaat aaattctcat atcattttct tgagggatta
       61 acgctcacgg aagatggaat tcttactcaa ggaaatgagc aagtttatat tccacagaaa
      121 gagttaggtg tattaatagt attacttgaa tccgctggtc atgttgtact gaaagatatg
      181 atcatcgaat cagtatggaa aaatattatt gttagtgacg agtccctgac aagatgtatc
      241 tattctttgc gctgcatttt tgaaaaaatt ggctatgatc gttgcataga aacaatctac
      301 cggaaaggtt atcgtttcag cgggcaggtt ttcaaaacta aaattaatga agataatact
      361 tcagactatt ccatagctat attccctttc actacttcat tgaatacact ggatccatta
      421 atacttaatc aggaattagt gcaaatcatt tcaaa
//
LOCUS       DAZOZD010000700          455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZOZD010000700 DAZOZD010000000
VERSION     DAZOZD010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.709"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>455
                     /locus_tag="ACHUPB_004978"
     CDS             <1..>455
                     /locus_tag="ACHUPB_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFR0761491.1"
                     /translation="RLIKNACLKLYNLAHTATNSHLKPTGPDNSDVLLFKKLFSPSKL
                     MTIIGDEIPLISEKQSLSKVLLNDENNELSDGTNFWDKNRQLTTDEIACYLQKIAANA
                     KNTQVNYPTGLYVPYSTRTHLEDALNENIKSDPSWPNEVQLFPINTGGH"
BASE COUNT          154 a          106 c           70 g          125 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acact
//
LOCUS       DAZOZD010000701          449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZOZD010000701 DAZOZD010000000
VERSION     DAZOZD010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.710"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>449
                     /locus_tag="ACHUPB_004979"
     CDS             <1..>449
                     /locus_tag="ACHUPB_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0761492.1"
                     /translation="RWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSET
                     VQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPES
                     NGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSAL"
BASE COUNT          111 a          107 c          146 g           85 t
ORIGIN      
        1 agcgatggtg ctctgacggg ttcgagttcc gctgtgataa cggagaaaaa ctgcgagtca
       61 cgttcgcgct ggactgctgt gaccgtgagg cactgcactg ggcggtcact acgggcggct
      121 tcaacagtga aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg
      181 agcttccggc gtctccagta gagtggctga cggataatgg ttcatgctac cgggctaatg
      241 aaacacggca gtttgcccgg atgttggggc ttgaaccgaa gagcacggcg gtgcggagtc
      301 cggagagtaa cggcatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagtg
      361 tcatgcccaa accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt
      421 ataacgaatg gcatccgcat agtgcactg
//
LOCUS       DAZOZD010000702          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZOZD010000702 DAZOZD010000000
VERSION     DAZOZD010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.711"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>448
                     /locus_tag="ACHUPB_004980"
     CDS             <1..>448
                     /locus_tag="ACHUPB_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0761493.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALAT"
BASE COUNT          139 a           96 c          108 g          105 t
ORIGIN      
        1 aagcgacaac ctgtaacatc acacttactg gtaataacgt cacgaatgat ggcaataata
       61 actacacatt gagaatccct aagatgggtc tggataagat cgcgaataaa acgacagaat
      121 ctcaggctga ttttaaactg gttgctagtg ggtgcagcag tggcatcagt tggattgata
      181 ccactctgac cggaaatgca tcatcaagct cacctaagct tattataccg cagtctggtg
      241 attcatcttc gacgacaagt aatatcggta tgggtttcaa aaaacggact actgatgatg
      301 ccactttcct taaacctaac agtgcggaaa agatacgctg gagcacagac gagatgcagc
      361 ccgataaggg tcttgaaatg accgttgcgc tacgtgaaac agatgcaggg caaggcgtac
      421 cggggaattt tcgtgcactg gcaacgtt
//
LOCUS       DAZOZD010000703          447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZOZD010000703 DAZOZD010000000
VERSION     DAZOZD010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.712"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          164 a           66 c           98 g          119 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctata
//
LOCUS       DAZOZD010000704          443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZOZD010000704 DAZOZD010000000
VERSION     DAZOZD010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.713"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..65)
                     /locus_tag="ACHUPB_004981"
     CDS             complement(<1..65)
                     /locus_tag="ACHUPB_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761494.1"
                     /translation="MLMSLAQCPGFLFAHREECTV"
BASE COUNT          108 a          118 c          111 g          106 t
ORIGIN      
        1 tccacggtgc attcctcacg atgtgcgaat aaaaagcccg ggcattgcgc cagagacatg
       61 agcatccggc accccggaca gggcgctata tgaccccggt aatgaatgct ctgaacatcc
      121 cgtaatgaaa aattgtgggg atgctatata cgttccggtg ggagtactgg gcactgaaat
      181 ccccaccggt cccagtcgtg agcctctgta tgactgcccc ctgacaagtc tgatgacttt
      241 atcaccgtca gcttctccct ggtacgcagc aataatcagc ccgccaatgt cagggtctcc
      301 ccatctgttg cagacagagc tcgccacgat tctgtaaata atatcttctg tggttatatt
      361 tattttcacc cagccagtca gtctggatat gggccagtag cagtagcggg tgtgataaat
      421 ggggccgtta atgccgtaaa aag
//
LOCUS       DAZOZD010000705          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZOZD010000705 DAZOZD010000000
VERSION     DAZOZD010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.714"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..120)
                     /locus_tag="ACHUPB_004982"
     CDS             complement(<1..120)
                     /locus_tag="ACHUPB_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761495.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEI"
     gene            complement(376..>441)
                     /locus_tag="ACHUPB_004983"
     CDS             complement(376..>441)
                     /locus_tag="ACHUPB_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFR0761496.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          128 a           90 c           87 g          136 t
ORIGIN      
        1 aatttctgca aactttcttt ttaccgtcca ataatcaata tttctattgt gattgttaac
       61 cactgcgtgc attggcggga ttgaagggtg aaaaatatgt gcaattggga agcctttcat
      121 tgatgcctgc cttgtcgtca tgacaaacaa taatagatgc ttattttcct cgcagggttc
      181 tttcgcacta tcaaaaaacg ctcatttacc cggcgttaca gtaaacgaag caacattctg
      241 tggcctacga cgatcaaaag ctctgtgaaa atttcccttg ttgtctttgt ctgaccttat
      301 cgcggcgatc agcaattatt cgttttgtga tcctcaacac aaaatgctat tcgtcgccta
      361 tgaggggaga agaatttatg gaaaacgatt gcattcacaa aatgacgtcc tgtcagaaga
      421 gaaaaagttg acgttaatca a
//
LOCUS       DAZOZD010000706          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZOZD010000706 DAZOZD010000000
VERSION     DAZOZD010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.715"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..440)
                     /locus_tag="ACHUPB_004984"
     CDS             complement(<1..440)
                     /locus_tag="ACHUPB_004984"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFR0761497.1"
                     /translation="MFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVGRFG
                     KDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTMSLV
                     GPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILS"
BASE COUNT          149 a          115 c           50 g          127 t
ORIGIN      
        1 ctagacaaaa tctcattttc atctaccatt tctatagatg ctaagtcagt tatccctggc
       61 ctaaccgata aaactttttc ccttatatca tcaggatact catcaataaa ctccctcact
      121 tccggtcttg ggccaaccaa actcattgtt ccagaaagaa catctatcaa ttgaggaagc
      181 tcatcgattt tgtacttccg taaataccat ccaactctgg ttacccgagc atcttgacca
      241 actgttatcc gtcctttttt ttctgaatcg ataaacatcg ttctaaattt atgtatccta
      301 aaatctttcc caaatctccc aactctatac tgcctaaaaa aaaccccccc tttagaatca
      361 gcaacaatta acattgacac aatgataaaa acaggaaata gaaaaagcag gcctaagcct
      421 gccacgataa catcaaaaat a
//
LOCUS       DAZOZD010000707          432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZOZD010000707 DAZOZD010000000
VERSION     DAZOZD010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.716"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHUPB_004985"
     CDS             <1..185
                     /locus_tag="ACHUPB_004985"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0761498.1"
                     /translation="SDIDGHLSMELQDWRKEPRKKYLVQIVGSFAEFERAMLRERTKS
                     GLAAARQDGLVDGDQS"
BASE COUNT          119 a          105 c          114 g           94 t
ORIGIN      
        1 catctgacat agatggacac ctgagtatgg aattacagga ctggcgaaaa gaacctcgta
       61 aaaagtattt ggtgcaaatc gttggttcct ttgctgaatt tgaacgcgcc atgcttcgtg
      121 aacggacaaa gagtggactt gcagcggcac gccaggacgg gttggtggat ggcgaccaaa
      181 gttaacaccc cagcaacaaa aagagattgt ctcgctcgtc acatcggggc agaaaacagg
      241 tgctgatgcc gcccgtctgt ttcgggttca cccttccacc gtggtgcgtc ttctcgccag
      301 acaccggatg actgagatca aacagacata atctatgcgg agatttacag cagggcatgg
      361 ttcacaaatc tgacagcgat gaattatcgg cactgcgggc agaaaatgct cgaatcatca
      421 agcccctgtt ac
//
LOCUS       DAZOZD010000708          426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZOZD010000708 DAZOZD010000000
VERSION     DAZOZD010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.717"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>426
                     /locus_tag="ACHUPB_004986"
     CDS             <1..>426
                     /locus_tag="ACHUPB_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0761499.1"
                     /translation="SGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYALMHSISRSQN
                     VNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYYKINLSKARI
                     SSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNA"
BASE COUNT          147 a           84 c           85 g          110 t
ORIGIN      
        1 gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt ctgtcggaaa
       61 caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc attccatctc
      121 aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg ataaatcctc
      181 accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg aatttatact
      241 ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc tgtcaaaagc
      301 ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag aactggaacc
      361 acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt tagccggtac
      421 gaatgc
//
LOCUS       DAZOZD010000709          425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZOZD010000709 DAZOZD010000000
VERSION     DAZOZD010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.718"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            30..206
                     /locus_tag="ACHUPB_004987"
     CDS             30..206
                     /locus_tag="ACHUPB_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761500.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          153 a           48 c          103 g          121 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaa
//
LOCUS       DAZOZD010000710          425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZOZD010000710 DAZOZD010000000
VERSION     DAZOZD010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.719"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHUPB_004988"
     CDS             complement(<1..229)
                     /locus_tag="ACHUPB_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFR0761501.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAP"
     gene            complement(226..333)
                     /locus_tag="ACHUPB_004989"
                     /pseudo
     CDS             complement(226..333)
                     /locus_tag="ACHUPB_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          103 a          125 c          102 g           95 t
ORIGIN      
        1 ccggggcaag ttccggcatc tcattacaca acagcacggg gtcaatcact cctgaaacac
       61 gggcctgtct gcagatgcct gtgtagatat cccgatacgc tcgtacagaa aaaatgtccg
      121 ccggtagtgt gatcagaata tccatcactt catgatctgc cccacgcaga aagaatgcgc
      181 caatgacagc gccttccagg tcatcattac gccagactgg tgttgtcatg cagccacacc
      241 tctgatacaa gaacggtagc tgggccagtt gaacgacaac cagttgcgcc ccccgtctgt
      301 gatcctgtcg gcaatgcggg ggctgatgaa cgctggattt gcccctatat ttccagacac
      361 ctgttatcac ttaacccatt actggcttgc tgccgtagat attcccgtgg cgagcgataa
      421 cccag
//
LOCUS       DAZOZD010000711          424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZOZD010000711 DAZOZD010000000
VERSION     DAZOZD010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.720"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            13..424
                     /locus_tag="ACHUPB_004990"
                     /pseudo
     CDS             13..424
                     /locus_tag="ACHUPB_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          106 a          120 c          101 g           97 t
ORIGIN      
        1 taaggttctt tcatggtcac agttaacctt cattgccccc gttgccagtc cgttcaggtt
       61 taccgtcacg ggcagaaccc aaaaggacat gatcgattcc gttgccgtga ctgtcaccgg
      121 gtgtttcagc tcacttactg ttacgaggcc cgtaaaccgg gagttaaaaa ccaaatcact
      181 gaaatggctt tcaacggtgc cggagttcgc gatactgccc gtacgctgaa aatcggcatc
      241 aataccgtca tacggacatt aaaaagctcc cgccccggcg gataacatct gcgccagtcg
      301 ctcacgctga tgttgccctt atctgtgaac ttgatgggca gtggagcttc gttggcagca
      361 aggcgcaaca acactgtcac gaacggtgca atagtgatcc acacccaacg cctgaaatca
      421 gatc
//
LOCUS       DAZOZD010000712          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZOZD010000712 DAZOZD010000000
VERSION     DAZOZD010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.721"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUPB_004991"
     tRNA            41..116
                     /locus_tag="ACHUPB_004991"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZOZD010000713          417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZOZD010000713 DAZOZD010000000
VERSION     DAZOZD010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.722"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(62..319)
                     /locus_tag="ACHUPB_004992"
     CDS             complement(62..319)
                     /locus_tag="ACHUPB_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR0761502.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          138 a           76 c           56 g          147 t
ORIGIN      
        1 agggactata tatttcgttt tcagataaga ctccttgaga gattatcttt tatgtaaaat
       61 ctcaaatatc acctgatgta atgtcaatga taatattaag tgcgctctga acaacatctt
      121 tcttggtgtg attaacatct gacaaaaaga acatcagctt ctttattatg ttcttgttgt
      181 ttattatttc acgctcacag aataaagcgt tgactgcctc ttctatgaca tttctctcgt
      241 aaaaaaattg agagttagcc tcatataaaa tattggccaa tttttcacca aaaaccatat
      301 tataataatc actgtgcatc ttcctccccc agggttcagt caataatttt accatgccta
      361 attatgacag gtgtaacaag catattttgc tatataaata aaatacataa tactgat
//
LOCUS       DAZOZD010000714          416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZOZD010000714 DAZOZD010000000
VERSION     DAZOZD010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.723"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..139
                     /locus_tag="ACHUPB_004993"
     CDS             <1..139
                     /locus_tag="ACHUPB_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR0761503.1"
                     /translation="QEMNTGVPGNKVMKKLPLSSLELSYSERIGDSKIWNMVKGYSSF
                     V"
     gene            149..>416
                     /gene="ipgE"
                     /locus_tag="ACHUPB_004994"
     CDS             149..>416
                     /gene="ipgE"
                     /locus_tag="ACHUPB_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR0761504.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGI"
BASE COUNT          141 a           56 c           90 g          129 t
ORIGIN      
        1 ccaagagatg aatactggtg tgcccggaaa taaagtcatg aaaaaattgc cgctatcttc
       61 gttagagcta tcttattctg aaagaatagg ggactcaaaa atatggaata tggtgaaagg
      121 gtattcgtca tttgtataag aggaatatat ggaagattta gcagatgtta tttgccgagc
      181 cttgggtatc cctttaattg atatcgatga tcaagcaatc atgcttgatg atgatgtgct
      241 aatatatatt gaaaaagaag gagattcaat aaatttatta tgcccttttt gtgcattgcc
      301 tgaaaatata aatgatctta tatatgcgtt gagcctaaat tactcagaaa agatatgctt
      361 agctactgat gatgaaggcg gaaatctaat tgcgcgatta gatcttaccg ggataa
//
LOCUS       DAZOZD010000715          413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZOZD010000715 DAZOZD010000000
VERSION     DAZOZD010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.724"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>413
                     /locus_tag="ACHUPB_004995"
     CDS             <1..>413
                     /locus_tag="ACHUPB_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0761505.1"
                     /translation="NNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKI
                     HELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEP
                     YILILLFSLLVVGLSDVILFSKPVPTAVFVTIILL"
BASE COUNT          130 a           78 c           72 g          133 t
ORIGIN      
        1 aaataacgat ttagttaatt attcgcatga taacaccaga acttcagtcg gtgcccgtct
       61 ggcaatgtat gaagttggct taaaaacata ttctccaata ggacaatcac tggaaaaacg
      121 agcggaaaaa atacatgaac tagaagaaaa agagcctaga ttgagtggcg ctttacccta
      181 tatagattct catttgcata acgatctcat agatacgtta tcaacgcgtg gtattcctgg
      241 agttgtatta acaattttag ccttttcagc aatactcata tatgccttaa gaactgctaa
      301 agaaccttat attttaatct tgcttttttc actactggta gtaggcctaa gtgatgtaat
      361 actcttttct aaaccggttc cgactgctgt gtttgtcacc ataatattgc ttt
//
LOCUS       DAZOZD010000716          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZOZD010000716 DAZOZD010000000
VERSION     DAZOZD010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.725"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUPB_004996"
     tRNA            54..130
                     /locus_tag="ACHUPB_004996"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUPB_004997"
     tRNA            173..248
                     /locus_tag="ACHUPB_004997"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZOZD010000717          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZOZD010000717 DAZOZD010000000
VERSION     DAZOZD010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.726"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>406
                     /locus_tag="ACHUPB_004998"
     CDS             <1..>406
                     /locus_tag="ACHUPB_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761506.1"
                     /translation="SYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAIAVERIK
                     EAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADNASLVNINLSVNKI
                     NTITFPKESKLENIYIDHNNLESLDLKNQHSLV"
BASE COUNT          149 a           73 c           65 g          119 t
ORIGIN      
        1 agttatcaac ttaataacgc attacctaac aaagaaacca tctccagcaa tttttgtgaa
       61 cgactagaac aatggggtaa taaatcggtt aataatggtg aagaaagagc aattgccgtg
      121 gagcgaatta aagaagctta caattcgaac atggcatctc ttgatttgtc atatcttgat
      181 ttgagtgaat taccgcctat cccctcgaca gttaatacgt taaatctgga aaacaattgt
      241 ctaacttgtc ttgacttagc tgacaatgcc agtctcgtta atatcaacct cagcgttaat
      301 aaaattaaca cgataacctt tccaaaagaa tcaaaactgg aaaacattta tattgatcat
      361 aacaatctgg aaagtttaga tttaaaaaat cagcattcat tggtta
//
LOCUS       DAZOZD010000718          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZOZD010000718 DAZOZD010000000
VERSION     DAZOZD010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.727"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(4..>406)
                     /locus_tag="ACHUPB_004999"
     CDS             complement(4..>406)
                     /locus_tag="ACHUPB_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0761507.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           85 a          110 c          136 g           75 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttct
//
LOCUS       DAZOZD010000719          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZOZD010000719 DAZOZD010000000
VERSION     DAZOZD010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.728"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>406)
                     /locus_tag="ACHUPB_005000"
     CDS             complement(<1..>406)
                     /locus_tag="ACHUPB_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761508.1"
                     /translation="GSVYTNAYRGPMETGKIALCSAFIFLVLFAKNELRTKIKFGKLI
                     LFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFR
                     HKTTLYTINFMLSLCAVIVTETRAAILVFPFFA"
BASE COUNT          142 a           68 c           86 g          110 t
ORIGIN      
        1 cgcaaagaat ggaaacacta atatggctgc acgcgtttca gttactatga cagcacataa
       61 gctaagcatg aagttaattg tatataatgt tgttttatgt ctaaagtcgg attttaaaat
      121 gagaattgat gccagtaaag aagggaaaag gatgatgtag cctgctgttg tagcgtggga
      181 agctgataat gcaacacggt caacgtttaa atagaaatgt tgccacatgg catgcgcaaa
      241 aaaaagtaac tgcgttgcca gggatgcgaa cagaattagt ttcccaaatt ttatttttgt
      301 tctcaactca tttttagcaa aaagaaccaa gaaaataaaa gcactacaca aggcaatttt
      361 cccagtttcc attgggccac gatatgcatt ggtataaaca gagcct
//
LOCUS       DAZOZD010000720          406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZOZD010000720 DAZOZD010000000
VERSION     DAZOZD010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.729"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..333
                     /locus_tag="ACHUPB_005001"
     CDS             <1..333
                     /locus_tag="ACHUPB_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0761509.1"
                     /translation="GNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLY
                     KLSYNYSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELL
                     NDNSEDNG"
BASE COUNT          143 a           61 c           70 g          132 t
ORIGIN      
        1 ggtaatataa cggacgcaga aatttattat aaatttttgt gcatttatgc gttcgaaaat
       61 catgagtatc taaaagattt tgcatcagta tgtcagccaa aaaagaaata tcaacaagca
      121 tatgaccttt acaaactaag ttacaattac tccccgtatg atgactattc agttatttat
      181 cgtatgggtc aatgtcagat tggggctaaa aatatcgata acgcaatgca atgtttctat
      241 cacattatta acaattgtga ggatgatagt gttaagagta aagcgcaggc atatattgaa
      301 ctcttaaacg ataattcaga agataatggc taagcattac aatcatttgg ttaaatttgc
      361 tggttatagt gcagttaact actataacca gcacctctta tgtttt
//
LOCUS       DAZOZD010000721          402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZOZD010000721 DAZOZD010000000
VERSION     DAZOZD010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.730"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..223
                     /gene="ipgF"
                     /locus_tag="ACHUPB_005002"
     CDS             <1..223
                     /gene="ipgF"
                     /locus_tag="ACHUPB_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR0761510.1"
                     /translation="EEMLISHPCLSVHYAAKLLNEFMMMYGRGWEAVGAYNAGTSPKK
                     KKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            232..>402
                     /locus_tag="ACHUPB_005003"
     CDS             232..>402
                     /locus_tag="ACHUPB_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR0761511.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIP"
BASE COUNT          139 a           58 c           97 g          108 t
ORIGIN      
        1 tgaggaaatg ctaattagtc atccatgtct ttctgtacat tatgcagcaa agttattaaa
       61 tgagtttatg atgatgtatg gaagagggtg ggaggctgtt ggagcctata atgcaggaac
      121 gtctccgaaa aagaaaaaag aaagattaaa gtacgcagag gatatctata gacgctattt
      181 gcggatagca gcagagagca agcagaataa tcgaaggata taagaggatt tatgtctgag
      241 gcaaagaact caaatcttgc accattcagg ttattagtga aactcacaaa cggtgtcggg
      301 gatgagtttc cgttatatta tgggaataac ttaattgttc tcggacggac gatagaaaca
      361 cttgagtttg gaaacgacaa tttcccagaa aatattattc ct
//
LOCUS       DAZOZD010000722          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZOZD010000722 DAZOZD010000000
VERSION     DAZOZD010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.731"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>401)
                     /locus_tag="ACHUPB_005004"
     CDS             complement(<1..>401)
                     /locus_tag="ACHUPB_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0761512.1"
                     /translation="LIEQINDLKMWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAIN
                     SICNPGDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLT
                     FVNACQEHDDNSLKLFASLLISLSYSSAYAD"
BASE COUNT          139 a           75 c           66 g          121 t
ORIGIN      
        1 atctgcgtag gcactagaat atgaaagtga aattaacagt gaagcaaata attttaagct
       61 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
      121 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
      181 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gagtcgcatc
      241 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
      301 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt caatatactt
      361 attgacccac attttgagat catttatttg ctcaattaaa a
//
LOCUS       DAZOZD010000723          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZOZD010000723 DAZOZD010000000
VERSION     DAZOZD010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.732"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          127 a          107 c           67 g          100 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt a
//
LOCUS       DAZOZD010000724          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZOZD010000724 DAZOZD010000000
VERSION     DAZOZD010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.733"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>401
                     /locus_tag="ACHUPB_005005"
     CDS             <1..>401
                     /locus_tag="ACHUPB_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0761513.1"
                     /translation="GQSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRS
                     LNDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAG
                     RINSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          112 g          119 t
ORIGIN      
        1 ggtcaatcta aaagtcttgg tgaatctctt ggtggcttga tgagcgatat gtcgcatggg
       61 gtcgattcac taaccgatat tagttacagg gtacgagcgt tctttgatga ggtgacatta
      121 gcttatcgtt cactaaatga cactcaaaaa gctgtagctg atggtcttgc aaatggcctg
      181 ttaagtgcat taaaggggct tgctggtata gtcgctgtac ggtctgggat tggtgctgta
      241 ggtggtatct ggaacttaat tagggccatt tccacgcttg gaaatgtggc aaatacagca
      301 gcaggaagaa tcaattcacg ttctactggt agtgttgaag gtggaaaaag taggctatct
      361 gtagctgatg caattaccag agtaatgatt gtcggtatga c
//
LOCUS       DAZOZD010000725          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZOZD010000725 DAZOZD010000000
VERSION     DAZOZD010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.734"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>399)
                     /locus_tag="ACHUPB_005006"
     CDS             complement(<1..>399)
                     /locus_tag="ACHUPB_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0761514.1"
                     /translation="KVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGF
                     IGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSA
                     DLFLETTDLPCWRHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           85 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatacttt
//
LOCUS       DAZOZD010000726          399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZOZD010000726 DAZOZD010000000
VERSION     DAZOZD010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.735"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(93..200)
                     /locus_tag="ACHUPB_005007"
     CDS             complement(93..200)
                     /locus_tag="ACHUPB_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0761515.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT           85 a          116 c           99 g           99 t
ORIGIN      
        1 gagataaaga caaaaatggc cgcaggctgt tacccctgcg gccggtttcg ggcgcatatt
       61 gccatcacgg cagcctgacg cccgttttca ccttacttcc ggttacgcca ccagccgaca
      121 atcgctgcgg taataatccc cgccaggatc ggtgctgcca ggtcgtgcca gaaaatcatg
      181 gcaaacttcg cgagcgtcat atagccgcct tgttgtaatg acaacatttt gcggctattc
      241 ttgaattgtt gatggttcaa gattagcccc cgttctgttg tcaggtttta cctctcaacg
      301 tgcgggggtt ttctctttcc agcaaccaat gccaccaggg attaagcccc cgcaacattg
      361 cgcctcaccg gataacgccg gcttggtgtg gatactacg
//
LOCUS       DAZOZD010000727          397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZOZD010000727 DAZOZD010000000
VERSION     DAZOZD010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.736"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUPB_005008"
     CDS             complement(<1..304)
                     /locus_tag="ACHUPB_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0761516.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          123 a           76 c           80 g          118 t
ORIGIN      
        1 ttgaatcctc tctcggcacg gttgcaatta gcgagtgggt agtatcacca acagcgcgaa
       61 atccgcgtct gataatggat atgttctgat ataacgtgtt agtaggcaca cgcatacctt
      121 cttcttccca gactctggtg aaaaaatctt gttgtgcaac cacatcagga aaagcttcaa
      181 gtagaagtga taaacatcga ctggctggcg ttgtcagaac gacgttaaga tcggcgttat
      241 gtaccgatat taattttcgg tgctcaggcc agaactcgat attatcgtta ataatccagt
      301 acatacgtca ataaacacta aatcaatcga aatggagatc acatagtttg ctaagtatat
      361 tggtatttac ggcataaata tatattaatt ttatatt
//
LOCUS       DAZOZD010000728          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZOZD010000728 DAZOZD010000000
VERSION     DAZOZD010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.737"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUPB_005009"
     CDS             63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUPB_005009"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0761517.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c          100 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcactgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DAZOZD010000729          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZOZD010000729 DAZOZD010000000
VERSION     DAZOZD010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.738"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            75..350
                     /locus_tag="ACHUPB_005010"
     CDS             75..350
                     /locus_tag="ACHUPB_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR0761518.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT           93 a           96 c           81 g          123 t
ORIGIN      
        1 agccatactg acgccttcaa tggatattga tattcactac agatattgtt cgcagcagac
       61 aggatgtatt taatatgcca acagttctct ctcgcatggc gatgcaactc aaaaaaacag
      121 cctggataat tcccgtcttc atggtttcgg gatgctcatt atctccggca atcccggtga
      181 tcggtgctta ttatcccagc tggtttttct gcgccattgc cagccttatt ttgatgctca
      241 tcacgaggcg agttattcag cgggcaaata tcaatctggc atttgtcgga attatttata
      301 ccgccctttt tgctctctac gccatgctgt tctggctggc atttttctaa tctatttgag
      361 acgcgctcat ggaaagtacg ccgaaaaaag ctc
//
LOCUS       DAZOZD010000730          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZOZD010000730 DAZOZD010000000
VERSION     DAZOZD010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.739"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>393)
                     /locus_tag="ACHUPB_005011"
     CDS             complement(<1..>393)
                     /locus_tag="ACHUPB_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0761519.1"
                     /translation="LTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLH
                     KMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDG
                     EQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT           99 a          109 c           74 g          111 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taa
//
LOCUS       DAZOZD010000731          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZOZD010000731 DAZOZD010000000
VERSION     DAZOZD010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.740"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            20..127
                     /locus_tag="ACHUPB_005012"
     CDS             20..127
                     /locus_tag="ACHUPB_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0761520.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           96 a           85 c          126 g           86 t
ORIGIN      
        1 ttacaataca ggcggctata tgacgctcgc agaactgggc atggccttct ggcatgattt
       61 agcggctccg gtcattgctg gtattcttgc cagtatgatc gtgaactggc tgaacaagcg
      121 gaagtaacgt gccatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc
      181 cgcaggggtg aaactctgcg gcctttttcg taagaaaaat gcaacggttg caagcggata
      241 atattctgcg gaatgcgcag cacagagtga agacgtgacg gattccggaa cagaaaaaca
      301 gtcgtagaat cagcgtcaag ccggaagggt tccggtgagg cgcaatgttg cgggggcttt
      361 atccctggtg gcattggttg ctggagagag aaa
//
LOCUS       DAZOZD010000732          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZOZD010000732 DAZOZD010000000
VERSION     DAZOZD010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.741"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            251..>389
                     /locus_tag="ACHUPB_005013"
     CDS             251..>389
                     /locus_tag="ACHUPB_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR0761521.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT          121 a           69 c           65 g          134 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctc
//
LOCUS       DAZOZD010000733          388 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZOZD010000733 DAZOZD010000000
VERSION     DAZOZD010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.742"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>388
                     /locus_tag="ACHUPB_005014"
     CDS             <1..>388
                     /locus_tag="ACHUPB_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFR0761522.1"
                     /translation="AAKVIENTQRDVNIALINELSIIFNKLGIDTLEVLEAAGTKWNF
                     LPFRPGLVGGHCIGVDPYYLTHKAQSVGYHPEMILAGRRLNDSMGQYVVSQLVKKMLK
                     QRIQVEGANVLVMGLTFKENCPDLRNT"
BASE COUNT          117 a           58 c           95 g          118 t
ORIGIN      
        1 gctgcaaaag taattgaaaa cacgcagcga gatgtcaata ttgcattgat taatgagtta
       61 tctattatat ttaataagtt agggattgat accttagagg ttcttgaggc tgcaggtacg
      121 aagtggaatt ttttaccttt taggcccggt ttagtaggtg gccactgtat aggtgtagat
      181 ccttattatc ttacacataa agcgcaaagt gtcggctatc atccggagat gattttagcc
      241 ggacgtcgtt taaatgatag tatggggcag tatgtcgttt cccagttagt caaaaaaatg
      301 ttgaaacaac ggattcaagt tgaaggggcg aatgtgttag tgatggggct tacatttaaa
      361 gagaattgcc cagatctacg aaacacta
//
LOCUS       DAZOZD010000734          386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZOZD010000734 DAZOZD010000000
VERSION     DAZOZD010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.743"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>386
                     /locus_tag="ACHUPB_005015"
     CDS             <1..>386
                     /locus_tag="ACHUPB_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761523.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAZOZD010000735          385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZOZD010000735 DAZOZD010000000
VERSION     DAZOZD010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.744"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>385
                     /locus_tag="ACHUPB_005016"
     CDS             <1..>385
                     /locus_tag="ACHUPB_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0761524.1"
                     /translation="VVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDG
                     DKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGS
                     LDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT          105 a           73 c           98 g          109 t
ORIGIN      
        1 gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta taacaaggat
       61 ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac tgatgataag
      121 cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat
      181 gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc
      241 aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat tagtgaattt
      301 ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc atggaccgat
      361 atgttgccag aatttggtgg cgata
//
LOCUS       DAZOZD010000736          384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZOZD010000736 DAZOZD010000000
VERSION     DAZOZD010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.745"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>384
                     /locus_tag="ACHUPB_005017"
     CDS             <1..>384
                     /locus_tag="ACHUPB_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFR0761525.1"
                     /translation="FRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVI
                     TLVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSD
                     EVMETLAHALRELRNAKKNFVLLVSV"
BASE COUNT          120 a           50 c           88 g          126 t
ORIGIN      
        1 ttccgacatg tgactataca gagaatttct gaggtattac aaagattgct tggagaaaat
       61 atttctgttc gcaatttaaa acttattatg gagtctttgg cgctttgggc tcccagagaa
      121 aaagatgtca taacattagt tgaacatgtc cgtgcatcac tttctaggta tatttgtagt
      181 aaaatagctg tttctggtga gattaaagtt gtgatgcttt ccggatatat tgaggatgca
      241 ataagaaaag ggataaggca aacctctggt ggctctttct tgaatatgga tatagaggtt
      301 tcggatgagg taatggaaac tttagcacat gctttgagag aattgagaaa tgcaaaaaaa
      361 aatttcgttc ttttggtatc agta
//
LOCUS       DAZOZD010000737          375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZOZD010000737 DAZOZD010000000
VERSION     DAZOZD010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.746"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..350)
                     /locus_tag="ACHUPB_005018"
     CDS             complement(<1..350)
                     /locus_tag="ACHUPB_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HFR0761526.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQ"
BASE COUNT          114 a           57 c           72 g          132 t
ORIGIN      
        1 ttttgaagtc ccttttcgtt ttcaaaatta agaccatttt gatgtactaa ggagtacaaa
       61 acatttgaaa attcgagaat ctcgcttttg gcatacgcat tcattgagtt ggaataaatg
      121 cttcttaaca catcttcttt ccctgaggaa gaaaataaaa aatcaagaca tttctgatta
      181 aaagacgttg caaagctttc tttttcagac actgatgctg gtgtttttag agaagtttgc
      241 gaactatgga tatcggatat tttgcttttt aactcgctgt attctgttga tgatggtgaa
      301 gttgccttat ataagaatgt tggcgcttga gtattattta cattatgcat ggcgcacctc
      361 agaaatggag aaaaa
//
LOCUS       DAZOZD010000738          374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZOZD010000738 DAZOZD010000000
VERSION     DAZOZD010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.747"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..336
                     /gene="istB"
                     /locus_tag="ACHUPB_005019"
     CDS             <1..336
                     /gene="istB"
                     /locus_tag="ACHUPB_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR0761527.1"
                     /translation="QLSYARVLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSN
                     KGFADWGEMFGDHVLATAILDRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDD
                     EMVESGQHQ"
BASE COUNT          125 a           76 c          101 g           72 t
ORIGIN      
        1 caactgagtt atgcccgggt gttgatcctg gatgaaatag gctatctgcc gatgaacaga
       61 gaggaagcca gtctgttctt ccggctactg aaccgtcgat atgaaaaagc gagcatcata
      121 ctgacgtcaa acaaagggtt cgcagactgg ggagaaatgt tcggagatca cgtactggca
      181 acagcgatcc tggatcggct gctacatcac tcaaccacgc tgaatatcaa aggagagagt
      241 taccggttaa aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt
      301 gatgatgaaa tggtggaaag cggacagcat cagtaacgaa agtatttagc gggcatgaaa
      361 atggcaaata acgg
//
LOCUS       DAZOZD010000739          369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZOZD010000739 DAZOZD010000000
VERSION     DAZOZD010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.748"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(166..>369)
                     /locus_tag="ACHUPB_005020"
     CDS             complement(166..>369)
                     /locus_tag="ACHUPB_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0761528.1"
                     /translation="NKCLIEATLKGFKSGELSFYEYRKNLATAGVFRWITNIHENKRY
                     YYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT           98 a           72 c           84 g          115 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttatt
//
LOCUS       DAZOZD010000740          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZOZD010000740 DAZOZD010000000
VERSION     DAZOZD010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.749"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(19..>367)
                     /gene="traA"
                     /locus_tag="ACHUPB_005021"
     CDS             complement(19..>367)
                     /gene="traA"
                     /locus_tag="ACHUPB_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR0761529.1"
                     /translation="LSVQGTSAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMMLFPEL
                     AMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVGFAII
                     SVFIAVGMSVVGL"
BASE COUNT          115 a          101 c           73 g           78 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa taaatacaga
       61 gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
      121 gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca tagctaactc
      241 agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc gaagcatatt
      301 cagacgagtg aatttagaaa aaaacgattt ctttttgacg ggcgcagaag taccctgaac
      361 acttaac
//
LOCUS       DAZOZD010000741          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZOZD010000741 DAZOZD010000000
VERSION     DAZOZD010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.750"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            95..>366
                     /locus_tag="ACHUPB_005022"
     CDS             95..>366
                     /locus_tag="ACHUPB_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0761530.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKD"
BASE COUNT          100 a           56 c           94 g          116 t
ORIGIN      
        1 aggcagtgtt gtgccgagtt atgcaagcgg ttaattcgtt gtgtatttaa ttatacaatg
       61 attccggtgt tctggaattt atttagagga atctatgcga gggaaaaaac gcattgggtt
      121 attgtttttg ctgatagcgg ttgtggttgg tggcggcggg ttattgctgg cgcaaaaagc
      181 cttacataaa acgtcggata cagcattttg cctttcctgc cactcgatga gtaaatcttt
      241 tgaggaatat cagggaactg tccacttttc gaaccagaaa gggatacgtg cggaatgtgc
      301 agattgccat attccaaagt cagggatgga ttatttattt gctaaattaa aagcatctaa
      361 agatat
//
LOCUS       DAZOZD010000742          364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZOZD010000742 DAZOZD010000000
VERSION     DAZOZD010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.751"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUPB_005023"
     CDS             complement(<1..242)
                     /locus_tag="ACHUPB_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0761531.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          113 a           66 c           74 g          111 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttac
//
LOCUS       DAZOZD010000743          363 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZOZD010000743 DAZOZD010000000
VERSION     DAZOZD010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.752"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..361)
                     /gene="ospG"
                     /locus_tag="ACHUPB_005024"
     CDS             complement(<1..361)
                     /gene="ospG"
                     /locus_tag="ACHUPB_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR0761532.1"
                     /translation="MIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFEDVNDSSA
                     LYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVPGTPLSD
                     IDTADIPDNIESLYLQLI"
BASE COUNT          114 a           80 c           67 g          102 t
ORIGIN      
        1 atatcaactg tagataaagg ctctctatat tatcagggat atcagctgta tcaatgtcac
       61 taaggggagt cccaggcacg cgcagcattc ggaggtacac atctccgcca tactgtataa
      121 caacggatgc ttcatcgcca taaaaagcat taaaaagctc agattcttgc caagccattt
      181 ccagaatctc attgtactgg ttgccaataa gatcatactt tttatacaaa gcagatgaat
      241 cattcacatc ttcaaagatt tctgctgtcg acccctgacc tattaactta ccgagaatgg
      301 gctccgttac tatgccagca tgagaattat tattctcaaa tggaaaaggt gtttgaataa
      361 tgg
//
LOCUS       DAZOZD010000744          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZOZD010000744 DAZOZD010000000
VERSION     DAZOZD010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.753"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>353)
                     /gene="mxiC"
                     /locus_tag="ACHUPB_005025"
     CDS             complement(<1..>353)
                     /gene="mxiC"
                     /locus_tag="ACHUPB_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR0761533.1"
                     /translation="VNAGINSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYI
                     EWLGNFGFNHRHTIVNFVEQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIF
                     MKKLESSSLLKDGSL"
BASE COUNT          117 a           73 c           47 g          116 t
ORIGIN      
        1 cttaaactgc catcttttag caagcttgag gattccagtt tcttcataaa aattacgtct
       61 gaagttctaa tcatcttaat tgcaattaat ttagataaca caaaaccaaa ctcataagca
      121 ttacagctcg gtttctcaga atccatgtct acaatcagtg actgctctac aaaattcaca
      181 attgtatgtc tgtgattaaa accaaaatta ccaagccatt ctatatactg atctgttgtt
      241 gatatgttcc ccatgataaa accacgataa catgcacgca aaagctgtgg ttcaagtttc
      301 attttgttgc caaataattt agcctgtata gctgaattta ttccagcatt aac
//
LOCUS       DAZOZD010000745          351 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZOZD010000745 DAZOZD010000000
VERSION     DAZOZD010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.754"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..124)
                     /locus_tag="ACHUPB_005026"
     CDS             complement(<1..124)
                     /locus_tag="ACHUPB_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR0761534.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
BASE COUNT          129 a           78 c           50 g           94 t
ORIGIN      
        1 tataaggggt tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg
       61 tcagcttttc cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg
      121 ccatggttct ttccaccatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga
      181 aaaaacacaa aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa
      241 actacaagac gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa
      301 ataattctgc tctatggttt acattcaaaa atatcattta tacttttcag a
//
LOCUS       DAZOZD010000746          348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZOZD010000746 DAZOZD010000000
VERSION     DAZOZD010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.755"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(81..308)
                     /gene="traY"
                     /locus_tag="ACHUPB_005027"
     CDS             complement(81..308)
                     /gene="traY"
                     /locus_tag="ACHUPB_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR0761535.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
BASE COUNT          103 a           82 c           46 g          117 t
ORIGIN      
        1 ttttcaacgg gcgcagaagc accctgaaca cttaaaacag tattcatatg attcctctga
       61 ttaatattga cagatgaaaa ctaaagttct ttaaatgttg actcgttctc ttcgatcacc
      121 tccctgaata tttcttcatt ataaaaatct ggataacgtt gcaaatgatc ctgcaacctc
      181 accaatgctt cgtttgtttt cgttcttcca ctacgctctc tggctccaag taataattta
      241 ttcgtttcct catcaagggt aagtaaaact tttctacttg gcgcagatct taaagctcct
      301 ttaatcaccc tccctcccat aaataaaaga gatttagtca aaatattc
//
LOCUS       DAZOZD010000747          347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.756, whole genome shotgun sequence.
ACCESSION   DAZOZD010000747 DAZOZD010000000
VERSION     DAZOZD010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.756"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>347)
                     /gene="yagA"
                     /locus_tag="ACHUPB_005028"
     CDS             complement(<1..>347)
                     /gene="yagA"
                     /locus_tag="ACHUPB_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFR0761536.1"
                     /translation="MAQHICRDAVEIHWANGNIQVIRPVRGISINGEAQGGIRPPYWV
                     ILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTLTALLNVASWLKRKPGTPE
                     LSLERPLFDTEVYV"
BASE COUNT           89 a           98 c           76 g           84 t
ORIGIN      
        1 acataaactt ctgtatcaaa caggggcctt tccagactta attccggcgt acctggcttt
       61 cttttaagcc agctggccac attcagaaga gcagtgagcg tgtttcgttc cagtttgctg
      121 tccacaggca ccggacatgt cagttgataa agagcatgag catacccttc actgcagatg
      181 attctgccat cggcactccg gcaaaaagca agaataaccc agtaaggggg acgtattccg
      241 ccctgcgctt caccattaat actgatcccc cgcacaggtc tgatgacctg aatattaccg
      301 ttcgcccagt gaatttccac agcatcacga cagatgtgct gcgccat
//
LOCUS       DAZOZD010000748          346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.757, whole genome shotgun sequence.
ACCESSION   DAZOZD010000748 DAZOZD010000000
VERSION     DAZOZD010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.757"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="istB"
                     /locus_tag="ACHUPB_005029"
     CDS             <1..268
                     /gene="istB"
                     /locus_tag="ACHUPB_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0761537.1"
                     /translation="LILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWH
                     GLMENPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          110 a           76 c           93 g           67 t
ORIGIN      
        1 tctaatcctg gacgatctgg ggctggaaca gttaagtaac gcccagtgta atgaccttct
       61 ggagatcaca gaggatcgtt acggacaaag cagcacgata gtggtaagcc agttcccggt
      121 agataaatgg catggtctga tggagaaccc aacaacagca gatgccatcc tggacagact
      181 ggtacataac tcacacagag tggtccttca gggagagtcc ctgaggaaaa atcccccgac
      241 agtggaaagc agcgaaaaaa cgagttaaaa agcggtagaa aaagttaagt tcccggttga
      301 tcgaatggat gcgaatcgct gatcgaactt cgatgccaat ccgtga
//
LOCUS       DAZOZD010000749          345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.758, whole genome shotgun sequence.
ACCESSION   DAZOZD010000749 DAZOZD010000000
VERSION     DAZOZD010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.758"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            51..260
                     /gene="fumD"
                     /locus_tag="ACHUPB_005030"
     CDS             51..260
                     /gene="fumD"
                     /locus_tag="ACHUPB_005030"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0761538.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          109 a           58 c           91 g           87 t
ORIGIN      
        1 tggttagatt gatatatgct gatagttggc atgataaata ggtataaaga atgggaaatc
       61 ggacaaaaga ggatgaactg taccgagaaa tgtgcagagt tgtcggtaaa gttgtgctgg
      121 aaatgcgcga tctggggcag gaaccaaagc atattgttat cgcaggcgta ttacgtactg
      181 cattagccaa taaacgcatt cagcgcagtg agctggaaaa gcaggcgatg gaaaccgtca
      241 ttaacgcact ggtaaaatga ccattcagga tgaggcggta agcttcatcc tgatattctt
      301 gcttcagttg cggtccagtt aatcattcta aataacaact gctga
//
LOCUS       DAZOZD010000750          333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.759, whole genome shotgun sequence.
ACCESSION   DAZOZD010000750 DAZOZD010000000
VERSION     DAZOZD010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.759"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          101 a           61 c           72 g           99 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta tta
//
LOCUS       DAZOZD010000751          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.760, whole genome shotgun sequence.
ACCESSION   DAZOZD010000751 DAZOZD010000000
VERSION     DAZOZD010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.760"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..303
                     /gene="ssb"
                     /locus_tag="ACHUPB_005031"
     CDS             <1..303
                     /gene="ssb"
                     /locus_tag="ACHUPB_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR0761539.1"
                     /translation="AQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTVQMLGRAPQQN
                     AQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQPPEGEDYGFSDDIPF"
BASE COUNT           84 a           98 c          102 g           48 t
ORIGIN      
        1 gcgcaggtct acatcgaagg tcagcttcgc acccgtagct gggaagataa cggtatcacc
       61 cgttacgtca ctgaaattct tgttaagacc acgggcaccg tgcagatgct gggacgtgca
      121 ccacagcaga acgctcaggc gcaaccgaag cctcagcaga atgggcagcc acagagtgct
      181 gacgcgacga aaaaaggtgg cgcgaaaacg aaaggccgtg gacgtaaggc cgcgcagcca
      241 gagcctcagc cgcaaccgcc ggagggggag gattacgggt tttcagacga catcccgttc
      301 tgatcgggct gactgtgaca accgccccgc cc
//
LOCUS       DAZOZD010000752          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.761, whole genome shotgun sequence.
ACCESSION   DAZOZD010000752 DAZOZD010000000
VERSION     DAZOZD010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.761"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..>332)
                     /gene="psiB"
                     /locus_tag="ACHUPB_005032"
     CDS             complement(<1..>332)
                     /gene="psiB"
                     /locus_tag="ACHUPB_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR0761540.1"
                     /translation="QSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPANWMMNGEYGS
                     EFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVVQVQHIFTPVA
                     ISHTLALAA"
BASE COUNT           78 a           99 c           90 g           65 t
ORIGIN      
        1 gcggcaagcg ccagcgtgtg actgatcgcg acaggcgtga agatatgttg cacctgaacg
       61 acggcgaaag gctgtccacc accattcacc agaaccagca tccagagctg agagacgtct
      121 cccggcgaac ataatgccag gtggaaacgt tcgtgggctg gcgtgaaacg aacctggacg
      181 gggaaaaagc ccccgaactc gctgccatac tctccgttca tcatccagtt agccggtgcg
      241 tccacttcac gcatcacctc gtgagtgaga ttacggcgca tatcgcttcc cgcagcacgg
      301 atttcttcat attcctgggc attcatggac tg
//
LOCUS       DAZOZD010000753          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.762, whole genome shotgun sequence.
ACCESSION   DAZOZD010000753 DAZOZD010000000
VERSION     DAZOZD010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.762"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(43..255)
                     /gene="cspH"
                     /locus_tag="ACHUPB_005033"
     CDS             complement(43..255)
                     /gene="cspH"
                     /locus_tag="ACHUPB_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0761541.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT           99 a           73 c           71 g           87 t
ORIGIN      
        1 tattccaata aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt
       61 tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat
      121 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc
      181 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
      241 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
      301 taaaataaag ccagcgatat ttaagaccgc
//
LOCUS       DAZOZD010000754          329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.763, whole genome shotgun sequence.
ACCESSION   DAZOZD010000754 DAZOZD010000000
VERSION     DAZOZD010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.763"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          102 a           59 c           64 g          104 t
ORIGIN      
        1 tttttattga aacaattaaa aatacgctga atattttcgg ccagtcactc atcttcacat
       61 aaaattttga ccatcatgcc gcatccctta gttgacaatt cattttcaat gatatgaata
      121 ttactaattt caccagataa tgttctcgtg gtcagcaggg tatgagtttt caccgtaatt
      181 ttaaggaaac acagtgcgtg gggattgaat aacagatagc cgacggaatt accccaccgg
      241 ctgaaagtca agttcaacga agatttttat tatctgaatt gggcagttta catcagtgtt
      301 cgatgtttat gaagacgata gatggcgag
//
LOCUS       DAZOZD010000755          326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.765, whole genome shotgun sequence.
ACCESSION   DAZOZD010000755 DAZOZD010000000
VERSION     DAZOZD010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.765"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(26..>326)
                     /locus_tag="ACHUPB_005034"
     CDS             complement(26..>326)
                     /locus_tag="ACHUPB_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761542.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          106 a           56 c           58 g          106 t
ORIGIN      
        1 agacgatcac aatgaaagga aaatattaac ggtttttgct atcactcaaa tgtttttcat
       61 cctgcaccat atcattgcca gattcatgca aagtatttat taatgcattg gcatctggat
      121 attgttgctg atacttggtt agatatatat cactatggat atgttcggca attatattga
      181 taagcaagcg attattcgag tataacccca gtgattcagc tcgttgtgaa aaaaccatta
      241 ctgtcttcat atcaacaaca agcataaaac ggtgagaagg gtaatattct tcttcatggt
      301 taaagcggta atacagttct acacgt
//
LOCUS       DAZOZD010000756          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.766, whole genome shotgun sequence.
ACCESSION   DAZOZD010000756 DAZOZD010000000
VERSION     DAZOZD010000756.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.766"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>325
                     /gene="ospB"
                     /locus_tag="ACHUPB_005035"
     CDS             <1..>325
                     /gene="ospB"
                     /locus_tag="ACHUPB_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR0761543.1"
                     /translation="LIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAE
                     SLSCILNSLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHY
                     RSTSIP"
BASE COUNT          101 a           63 c           55 g          106 t
ORIGIN      
        1 cttattgatg tacaaacaat catttcaaga atgaaagact gtggtattct aaatgtgaaa
       61 gatatccgtt ttacttcatg cggctccgct gataaagtgg ctcctaaaaa ttttaacaat
      121 gcccctgctg aaagtctttc ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa
      181 tctttgctag agcagataaa aaaacacctt gaaaacgatg agtcattgag tgatggtcta
      241 aaaatatccg gctatcatgg atatggagtt cactatggtc aagagctttt tccctactca
      301 cattatcgtt caacttcaat tcctg
//
LOCUS       DAZOZD010000757          325 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.767, whole genome shotgun sequence.
ACCESSION   DAZOZD010000757 DAZOZD010000000
VERSION     DAZOZD010000757.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.767"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
BASE COUNT          101 a           70 c           59 g           95 t
ORIGIN      
        1 tcatatcatc tcctttccat tggttaattt atcggaaatt catccccact aactaaaacg
       61 atccacacaa taaaactaaa tctaattgca ggcaatcaat tccagtcgat atatcatctg
      121 tatggaaatc atcgctcatc actcatcgca gcctgaaaac ccagagcaac ggggcaggtc
      181 tgcatataag tggttacacg gcagaattcc agagggttct gtcactaaat tcagtcactg
      241 acaaatttgc atgatgttga atagacgaga ttttgtgtcg aaatagttgt ttcagtaact
      301 gtcactcaat cggtggttta tgtga
//
LOCUS       DAZOZD010000758          324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.denovo.768, whole genome shotgun sequence.
ACCESSION   DAZOZD010000758 DAZOZD010000000
VERSION     DAZOZD010000758.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.denovo.768"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..>324
                     /locus_tag="ACHUPB_005036"
     CDS             <1..>324
                     /locus_tag="ACHUPB_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761544.1"
                     /translation="QKNILRVTNSSSSGISEKHLDHCANTVKNFLRKSIAAQSYSKMF
                     SQGTSFKSLNLSIEAPSGARSSFRSLEHLDKVSRHYLSEIIQKTHPLSSDERHLLSII
                     INSDF"
BASE COUNT          107 a           66 c           48 g          103 t
ORIGIN      
        1 acaaaaaaat atattacgcg taacaaactc atcttcttct ggcataagtg aaaaacattt
       61 agatcactgt gctaatactg tgaaaaactt tttaaggaag tctatagcag cgcaaagtta
      121 tagcaaaatg ttttctcaag ggaccagttt caaatcacta aatctttcta ttgaagcgcc
      181 atctggagca cggtcatcat ttcggtcact tgagcacctc gataaggtgt ccagacacta
      241 tctttctgaa ataatacaaa aaactcatcc tctttcttcg gatgaacgtc acttgctctc
      301 tattattatt aattctgatt ttaa
//
LOCUS       DAZOZD010000759         7254 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.206, whole genome shotgun sequence.
ACCESSION   DAZOZD010000759 DAZOZD010000000
VERSION     DAZOZD010000759.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.206"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHUPB_005037"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHUPB_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUPB_005038"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUPB_005038"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0761545.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUPB_005039"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUPB_005039"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0761546.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUPB_005040"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUPB_005040"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0761547.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUPB_005041"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUPB_005041"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0761548.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUPB_005042"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUPB_005042"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0761549.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHUPB_005043"
     CDS             5099..6232
                     /locus_tag="ACHUPB_005043"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0761550.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHUPB_005044"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHUPB_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0761551.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUPB_005045"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUPB_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0761552.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
BASE COUNT         1835 a         1887 c         1873 g         1659 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggt ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attt
//
LOCUS       DAZOZD010000760         6733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.218, whole genome shotgun sequence.
ACCESSION   DAZOZD010000760 DAZOZD010000000
VERSION     DAZOZD010000760.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.218"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            65..853
                     /locus_tag="ACHUPB_005046"
     CDS             65..853
                     /locus_tag="ACHUPB_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR0761553.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            1059..1406
                     /locus_tag="ACHUPB_005047"
     CDS             1059..1406
                     /locus_tag="ACHUPB_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR0761554.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            1400..2239
                     /gene="sul1"
                     /locus_tag="ACHUPB_005048"
     CDS             1400..2239
                     /gene="sul1"
                     /locus_tag="ACHUPB_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR0761555.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            2367..2570
                     /locus_tag="ACHUPB_005049"
     CDS             2367..2570
                     /locus_tag="ACHUPB_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761556.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            2726..3931
                     /gene="chrA"
                     /locus_tag="ACHUPB_005050"
     CDS             2726..3931
                     /gene="chrA"
                     /locus_tag="ACHUPB_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR0761557.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            3942..4247
                     /locus_tag="ACHUPB_005051"
     CDS             3942..4247
                     /locus_tag="ACHUPB_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR0761558.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            4474..5238
                     /locus_tag="ACHUPB_005052"
     CDS             4474..5238
                     /locus_tag="ACHUPB_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR0761559.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(5731..6315)
                     /gene="mphR(A)"
                     /locus_tag="ACHUPB_005053"
     CDS             complement(5731..6315)
                     /gene="mphR(A)"
                     /locus_tag="ACHUPB_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR0761560.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(6315..>6733)
                     /gene="mrx(A)"
                     /locus_tag="ACHUPB_005054"
                     /gene_synonym="mrx"
     CDS             complement(6315..>6733)
                     /gene="mrx(A)"
                     /locus_tag="ACHUPB_005054"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR0761561.1"
                     /translation="VGARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLL
                     TGSVSPILNAAIYNRTPPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVV
                     SAVFAGLVALLSLRLQFATMAAAATASAPTHTEGEH"
BASE COUNT         1344 a         1921 c         1998 g         1470 t
ORIGIN      
        1 ctgcgcaaaa gtattccaca aagccgcaac ttaaaagctg ccgctgaact taacgttagg
       61 catcatgggt gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg ctcgcgcggt
      121 gatcgagcgc catctggctg cgacactgga cacaatccac ctgttcggat ctgcgatcga
      181 tggagggctg aagccggaca gcgacataga cttgctcgtg accgtcagcg ccgcacctaa
      241 cgattcgctc cggcaggcgc taatgctcga tttgctgaaa gtctcatcac cgccaggcga
      301 tggcggaaca tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag tagtgccttg
      361 gcgctatccg gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg acatcctttc
      421 cggaacgttc gagcctgccg ttctggatca cgatcttgcg attttgctga ccaaggcgag
      481 gcaacacagc cttgcgcttc taggcccatc cgcagccacg tttttcgagc cggtgccgaa
      541 ggagcatttc tccaaggcgc ttttcgacac tattgcccag tggaatgcag agtcggattg
      601 gaagggtgac gagcggaacg tcgttcttgc tcttgctcgc atttggtaca gcgcttcaac
      661 tggtctcatt gctcctaagg acgttgctgc cgcatgggta tcggagcgtt tgcctgccga
      721 gcatcggccc ctcatctgca aggcacgcgc ggcgtacctg ggtagcgagg acgacgacct
      781 agcaatgcgc gtcgaagaga cggccgcgtt cgttcgatat gccaaagcaa cgattgagag
      841 aatcttgcgt tgagcggcat gtgcgaaaag tgcatcgacc cgcgccgagg gcatctgatg
      901 cctaactcgg cgttcaagcg gacgggctgc gcccgccgct caactatgcg ttagatgcac
      961 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     1021 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
     1081 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
     1141 ctaagcttgc cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt
     1201 ctctggttct gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg
     1261 tcgtcataat tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct
     1321 ttgtaggtat ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt
     1381 cgctgcggag gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgaggactc
     1441 cttcttcgat gagagccggc ggctagaccc cgccggcgct gtcaccgcgg cgatcgaaat
     1501 gctgcgagtc ggatcagacg tcgtggatgt cggaccggcc gccagccatc cggacgcgag
     1561 gcctgtatcg ccggccgatg agatcagacg tattgcgccg ctcttagacg ccctgtccga
     1621 tcagatgcac cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct atgcgctcaa
     1681 gcgcggcgtg ggctacctga acgatatcca aggatttcct gaccctgcgc tctatcccga
     1741 tattgctgag gcggactgca ggctggtggt tatgcactca gcgcagcggg atggcatcgc
     1801 cacccgcacc ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc ggttcttcga
     1861 ggcgcgggtt tccgccttgc gacggagcgg ggtcgctgcc gaccggctca tcctcgatcc
     1921 ggggatggga tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc tgtcgaacct
     1981 tcaaaagctg aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc ggaaatcctt
     2041 cttgggcgcc accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc ttgcggcgga
     2101 acttcacgcg atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg gagatctgcg
     2161 aagcgcaatc accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg ccagagaccg
     2221 agggttagat catgcctagc attcaccttc cggccgcccg ctagcggacc ctggtcaggt
     2281 tccgcgaagg tgggcgcaga catgctgggc tcgtcaggat caaactgcac tatgaggcgg
     2341 cggttcatac cgcgccaggg gagcgaatgg acagcgagga gcctccgaac gttcgggtcg
     2401 cctgctcggg tgatatcgac gaggttgtgc ggctgatgca cgacgctgcg gcgtggatgt
     2461 ccgccaaggg aacgcccgcc tgggacgtcg cgcggatcga ccggacattc gcggagacct
     2521 tcgtcctgag atccgagctc ctagggatcg cctcagaaaa cggaaaataa agcacgctaa
     2581 gccgtaagta agcgtgctcc tgtgaaagcc acagctaaaa ctgcgtagta cacatagagg
     2641 tatttttttc tagatttata tcgaatttcg ttatcgaatt tcgcttatta ttgatcaact
     2701 cgttgcagat catgaaggcg aaagtatgac gaataaccct accgatgaca gcagaccatg
     2761 gtcggtcttt cttatttttc tgcggcttgg attgacatct tttggcggcc ccattgcgca
     2821 cttgggctac ttccgcgccg aatttgtcac acggcggcgc tggctctccg aacggagcta
     2881 tgctgacttg gtcgcgcttt gtcagttctt gccagggcct gcaagcagcc aggtcggcat
     2941 agcggtagga ctgtctcggg ctggatacag cggggcgctg gctgcttggg ctggcttcac
     3001 gctgccgtct gccatagcct tgatcctttt tgcgctcggc atctccagct atggcgatta
     3061 cgtctcgcag ggcgcgttgc atggcttaaa agtggtggct gtggccgtgg tcgctcaagc
     3121 agtatggggc atggcgcgta acctatgcac ggatgggctg cgagtcacca tcatggcaat
     3181 tgctacctgc gtcgttttac ttgtgccgtc cgcgtgggga caggttggcg tgattgctat
     3241 cgcaggcatc gcaggccggt tattgttcaa gccagcgaaa gttgttgagc atgaccccct
     3301 acctatcacg gtcagtcacc gggccggcgt gctttggctc tcgctgttct ttgtcttgct
     3361 gattggcctg ccggtgttgg ccgaactgat gccaagtcaa accatggcaa tggtggattc
     3421 cttctatcgt gtcggatcac tggtgttcgg cggtggtcac gttgtgctgc cattactgca
     3481 agccgaagtg gtgccctccg gctgggtcaa caatgaatcc tttctcgcgg ggtacggggc
     3541 agctcaagcg gtgcccggcc ctttgttcac gttcgccgcg tttcttggtg cctcgatgaa
     3601 caccgccccg tcgggctgga tcggcggcat tgtgtgtctg ctggctatct tcgcgccctc
     3661 gttcttgctg gtcgtcggat caatgccatt ttgggagcgt ttgcgccgca atacaggcat
     3721 ccaagctgcg ctggccggga tcaatgccgc tgtagtcggc ttgctgctgg ccgcgctgta
     3781 tcagcctgta tggactagcg ccatctttca gccgcaagac ttcggcttgg cattagttgc
     3841 ccttgtcgca cttatgttct ggaagctccc gccgtggctg gttgtcctcg gtagcggtgc
     3901 agctgggtgg ctgttgagcg tcgctctgtg aggcacaaaa aatgactgac aaagacctct
     3961 acggcggttt gatccgcctg cacatccttc accatgcagc cgaggaacct gtctttgggc
     4021 tggggatcat cgaagagcta cgccgacacg gctacgagat gagcgctggc accgtgtacc
     4081 cgatgctgca cggcctggaa aaaaaaggct atctgacctc acgccacgaa cgcaccgggc
     4141 gacgtgaacg gcgtgtttat gacatcactg aacaaggcag aactgcactt gctgatgcga
     4201 aaacaaaggt caaggagctg ttcggagagt tggtagaagg tggttgagtt tttgcttcta
     4261 tgtcaggttg agctataccc taactggatt gtcattttca gaagacgact gcaccagttg
     4321 attgggcgta atggctgttg tgcagccagc tcctgacagt tcaatatcag aagtgatctg
     4381 caccaatctc gactatgctc aatactcgtg tgggctctgt tgcaaaaatc gtgaagcttg
     4441 agcatgcttg gcggagattg gacggacgga acgatgacgg atttcaagtg gcgccatttc
     4501 cagggtgatg tgatcctgtg ggcggtgcgc tggtattgtc gctatccgat cagctatcgc
     4561 gaccttgagg aaatgctggc ggaacgcggc atttcggtcg accatacgac gatctatcgc
     4621 tgggtccagt gctacgcccc ggagatggag aagcggctgc gctggttctg gcggcgtggc
     4681 tttgatccga gctggcgcct ggatgaaacc tacgtcaagg tgcggggcaa gtggacctac
     4741 ctgtaccggg cagtcgacaa gcggggcgac acgatcgatt tctacctgtc gccgacccgc
     4801 agcgccaagg cagcgaagcg gttcctgggc aaggccctgc gaggcctgaa gcactgggaa
     4861 aagcctgcca cgctcaatac cgacaaagcg ccgagctatg gtgcagcgat caccgaattg
     4921 aagcgcgaag gaaagctgga ccgggagacg gcccaccggc aggtgaagta tctcaataac
     4981 gtgatcgagg ccgatcacgg aaagctcaag atactgatca agccggtgcg cggtttcaaa
     5041 tcgatcccca cggcctatgc cacgatcaag ggattcgaag tcatgcgagc cctgcgcaaa
     5101 ggacaggctc gcccctggtg cctgcagccc ggcatcaggg gcgaggtgcg ccttgtggag
     5161 agagcttttg gcattgggcc ctcggcgctg acggaggcca tgggcatgct caaccaccat
     5221 ttcgcagcag ccgcctgatc ggcgcagagc gacagcctac ctctgactgc cgccaatctt
     5281 tgcaacagag cccgccgtgc tagtctgctc ggtgatggtg gagtgaagcc aacccgcaat
     5341 cgggttatga atctgcatcg cgattcgcaa tcagctgtct cttgagcatg tcgaactcct
     5401 gcgatgttat ctcgccgtgc tcgtgctgct acaccaactt gtccaactca tccgccacac
     5461 cgctccccac cacctgcgat ggccaggttg tttgctgggc gcattggcgg agtatcgtct
     5521 cgacccggga cacccacacc ggcacaacct tacgggagtg attcactgtc aaagaatcgg
     5581 cccggtgctc tgacgcaagt atcgggatgg tcaccatttg taagccgtag acctgagtgg
     5641 tgatcaagac ttcgatacca ccgaccgtac cggtactaat cgacgacggt cgtgttcgtc
     5701 gcctgccgca gggactctgc acacctccgt ttacgcatgt gcctggagga gttggaaatc
     5761 gtcgtgttcg ggaaacatta aacacaggat ggcagcgatc tgagccagca catgatcagc
     5821 tagctcacca tccggatcga cggcccactg catcgtcgcg ccagcgatga ccgagtgcag
     5881 gagcaactca gctgccgcag gagcacctgg gggcagtcgc ttgcggatcc cctccaccac
     5941 cgcgcggttc cgctggatcg caagcgtgcg tagctccggc acctggagct cgtaccagga
     6001 gatgagatag ttcaccgaga agtcgttgcg agtgttcatg ctccgaacga gcacctgcaa
     6061 aaattcccag agcccttgcg gccctgcgcc tatcggtatc gcattcaggt aatgccgcac
     6121 ctgctcgacg ccgcgctcca tcatcctcac cagcagcgta tcgcggttgg tgaagcgctg
     6181 gattaacgct gcgcgggaga gccccacctc ctttgctact ccgctgagcg tgaactctat
     6241 gggaccgcaa cgcttcagca ctacggtggc ggcctcgagt acctcgtcat cggacttgag
     6301 cttggggcgg ggcatcagtg ttcaccttct gtatgggttg gggcggaggc tgtggctgcc
     6361 gccgccattg tagcaaattg aagacggagc gagagtagag ccacgagccc cgcaaacacg
     6421 gccgatacaa cgaggccagg gagcggacca gcaaggtcga caaacgcgcc ggccgcaagc
     6481 ataaccatgg gcgaggctga cagcatcacc gccgagaccg tgccgagtac ccggccgaga
     6541 agttctggcg gcgtgcggtt gtagatggca gcgttgagaa tgggagagac tgagccggtc
     6601 agcagtccca cgagcgcgcc caacaacatc agcaccggca cgcctggcaa ctgtgaaagc
     6661 agaagcgagc ccaccgcaga gccacaaaat gccaccgcca gccagttctg cgctgatatc
     6721 cgggcgccga ccg
//
LOCUS       DAZOZD010000761         6382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.231, whole genome shotgun sequence.
ACCESSION   DAZOZD010000761 DAZOZD010000000
VERSION     DAZOZD010000761.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.231"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUPB_005055"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUPB_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUPB_005056"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUPB_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0761562.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUPB_005057"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUPB_005057"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0761563.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUPB_005058"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUPB_005058"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0761564.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUPB_005059"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUPB_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0761565.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHUPB_005060"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHUPB_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0761566.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..>6382)
                     /gene="phoE"
                     /locus_tag="ACHUPB_005061"
     CDS             complement(5330..>6382)
                     /gene="phoE"
                     /locus_tag="ACHUPB_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0761567.1"
                     /translation="KKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYMS
                     DNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLK
                     YKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFG
                     VIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNL
                     QSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFD
                     FGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKL
                     NINNDDIVAVGMTYQF"
BASE COUNT         1553 a         1572 c         1639 g         1618 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatagctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt tt
//
LOCUS       DAZOZD010000762         4039 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.317, whole genome shotgun sequence.
ACCESSION   DAZOZD010000762 DAZOZD010000000
VERSION     DAZOZD010000762.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.317"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUPB_005062"
     CDS             <1..122
                     /locus_tag="ACHUPB_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761568.1"
                     /translation="RTLAEHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            119..595
                     /locus_tag="ACHUPB_005063"
     CDS             119..595
                     /locus_tag="ACHUPB_005063"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFR0761569.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETVFTQRASEEEPGFSQIVYRRS"
     gene            595..1239
                     /locus_tag="ACHUPB_005064"
     CDS             595..1239
                     /locus_tag="ACHUPB_005064"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0761570.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            1301..1720
                     /locus_tag="ACHUPB_005065"
     CDS             1301..1720
                     /locus_tag="ACHUPB_005065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761571.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            1730..2287
                     /locus_tag="ACHUPB_005066"
     CDS             1730..2287
                     /locus_tag="ACHUPB_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFR0761572.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMEACSAWVAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            2448..3257
                     /locus_tag="ACHUPB_005067"
     CDS             2448..3257
                     /locus_tag="ACHUPB_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761573.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESGEAAENEPEQL
                     GIIEVEDCVEVNDELKVQQSNDSNTNKKTRAARGPSFTLSDKDDSFFNKAGLEKDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            3440..4033
                     /locus_tag="ACHUPB_005068"
     CDS             3440..4033
                     /locus_tag="ACHUPB_005068"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFR0761574.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGIERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATEYANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRPSKKAA"
BASE COUNT         1156 a          826 c          972 g         1085 t
ORIGIN      
        1 agcgcacatt agcggagcat aaaggcgatc tgcttatcgc tgcgctggtg cacgatcctc
       61 gactccgtgc cgctttagct gactctcatc atagaccggt catctatgag gtgagcaaat
      121 gaagatgata gctgctgttg gccgtaacta tgagatcggc atagcgaacg aactgccctg
      181 gcgttgttct accgatctga agctatttaa gagactcacc aaaaacgcca ctgtcgttat
      241 ggggcgtaaa acgatggaaa gtctcaaacg ccctcttcca gagcgtcata acctcgtttt
      301 gacgcgctct catggctttg taccaaatgg attctaccct gctggtgtgg atgatgtgtt
      361 gcgattacca gagcctgtgt gggtgattgg cggggaacaa atttactcgc tattcatgcc
      421 gcatgttgaa gagatttggc tctcccacat cggcgttgat gtgccaaacg ccgatgcatt
      481 cttcccggca agcatgatgc gtaatttagg ctttgtgcct gttgaaacag tttttaccca
      541 acgagccagc gaggaagagc ctggcttttc gcagatcgta tacagaaggt cgtaatggat
      601 taccggattg ggatcactgg tgctcagggc agtgggaaaa caaccctggc aaaatatatc
      661 gacaaacatt acggaatccc ttacgtggat gctggtgtcg gaagtttgat gagccgactc
      721 ggtgttcgag taggtgagtc tatgcctcta tatgagcggc ttcagattca aatggaaatc
      781 gcaaagcata tagagctact tacgcgtggt gttgaaggct ttgttatcga tcgcacacct
      841 gctgatgtta tggcctacac gttggatttg gtcggccata ccaatgaaga tcgatgtatt
      901 gagttagccc tcgatatcga aaagttttgc cacaaaactg ctatttcaaa ctttaacgcc
      961 attgctggcc tacgcccggg agtcgctctc tcagagcgag attacttgcg atcacaacga
     1021 gcatcattag accgtctgta tgtcgctcgt attgatgcgt tgatgtgcgg ggaactgaca
     1081 aaaattcacc tgcatccgca aaggggggat ctgcaaacct tcgtcgtttc caaccgatat
     1141 cgcacggttg aagcaagagc cagatcagtg atgagaatgc tagataacgc tgtagaaaag
     1201 atagaaaacc ggttctgtgg tcgagtgacc gttcattaga aattgttcgc ctcttcgaca
     1261 ttgcgacaat aaaactctca aaatgggtta aggataaaaa atgtttagtg aaatgttgct
     1321 tgaagatgaa ctggatcgga aaacaacaga ggctttgatt cgtgtagcgg acgaacattc
     1381 ccggtcgctt atgagcgatc gagaggctcg tctggctatt cgtgccatat tcgaaactgc
     1441 gcaggggctt gttggcgcac aagtaggtga agccattaac atcgccatgt ctcagttcag
     1501 tgaagacagt aaaaagcctc tgtttcctat gcatttgatg ctggctggtg gcacggtgct
     1561 ttatatctct gtttgtctgg atagcaacca aatcaatatt ctcaacactg cgtcaggtaa
     1621 gtggaaagat ccgattgtct gtgaaaccag tgaagaaact ttgaaaaaag cggctcaatt
     1681 tgtacgtagc gcactactta agggcgctaa gaagttgtaa ggagttctga tgacaacgat
     1741 tgttgcaggc atcgatatcg agtctacggg actggatttc cttgctggtc ataaaattat
     1801 tgaaatcgca attacccgct atgaactgga gacacagaga catattgata gtctggagat
     1861 gcgttttaac cctcgcagaa acatagatcc gaaagctcaa gccgttcatg gcatttcatt
     1921 agaacagctc gcagctgaac ctttgttgtc aaatcatgct agcgaaattg gcgcttatat
     1981 ggaggcatgt agtgcgtggg ttgctcataa cggcgaagcg tttgatatac catttattag
     2041 acacgagttt tcagggtatg gagtaagact gccagacgtt cccgttatag atactatgtt
     2101 atcgggattg tgggccacag aagacggtaa acgtcctcgc cttgaagaat tggccttctc
     2161 tcttggcttt atatacgatc atgccaaagc acatagtgcc ttatatgaca caaacttaat
     2221 gatgcaatgc ttctttaagg cacgtaataa atacggattt tttaaattac cctctgaaat
     2281 tgtgtaaaac aaaagcctgc tttaaagaga gtttgaagca ggctttcttt taaagaacag
     2341 tcgcctttca atcatttcct gcctgtattt aatacttttc cgcctgatag gtttagtcaa
     2401 aatgtagcca tcgaaacgca aatgcaacta aacagaagga gacttacatg agttcggttg
     2461 aaaatgtaat gacaaatgat gatctggatg agctgacagc catgttgcaa tcacttgatg
     2521 aaccagtaaa aaaagctgca caggttgaaa atactgatga tattgacgat ctgcttctcg
     2581 gcctcgatgc tggcgtagcc atgagttctg atgatgttgc cgaagaactg ttcaatgaag
     2641 aaaaagcagg tgatttcagc tctgctttaa atgagttgga gttagcgcat gagcctataa
     2701 acgtaattaa cgctgaaagt ggtgaagctg ccgaaaatga gccagaacag ttggggatca
     2761 ttgaggttga agattgtgtt gaggttaatg atgaattaaa agttcaacag tcaaatgata
     2821 gcaatacaaa taaaaaaact cgtgctgcaa gaggtcctag ttttacttta agtgataaag
     2881 atgattcgtt tttcaataaa gcaggcttag aaaaagatat tttcttagac gcttacgaaa
     2941 acgcgcctgt caaagcgaag gataagatat taaaccttct taattggttt agcggaggtc
     3001 cagatattag tgtttacacg gtaatttcca tgagacacct tcttacagaa aagaaggcta
     3061 caagtaatag tattaagatt gctttaatga gcaatccaga aaaaccgtat ccgcttaaca
     3121 ctgcgtcaac tcaggctggg caaatgatgg ctgtatttcc agctacagga attgccgtta
     3181 gagacggtgg aaatctaaca ttgaacgaag aatcaccgat cgttaagaag tttgtcgcgg
     3241 agtacactat tggatgacgt tcccctactg aaaataaagc ccatagagag ctttatagtg
     3301 ctgggtaaga taacccatac ccagcaccaa aaaaacgcgc cagagagctt cttgttcgca
     3361 tttctggcgc gttttatttg attgccatac ataaaatcaa atgcaaaaat aggtatttac
     3421 ttacctatcg agaaagaaga tgattgcagc cgaaaaaatc aaacagcgaa agcgcgacaa
     3481 ctctcttcgt gacctctgga gaacacctga ttggctgttt tctgccattc aacgttatct
     3541 tggagtgaca tttgatgttg acgttgcctg caacaaggac aacgcaaagc tgcctaattt
     3601 cataggcatt gagcgtgatg ctttgaaatc tgaatgggga cagccaggta caattgcctt
     3661 cctcaatcca ccctactcca aaatctcccc ctggattgat gctgctatac gtgagcaggc
     3721 tcgcggagtt actacagtga tgctaattcc tcaatccctc gatacaaagt ggtatgagcg
     3781 tgcaacagag tatgcgaatg agacgattat tctgtctggt ggccgcgtag cgtttgtcga
     3841 gcctgacgtc aatttgggtc aggtagaagt aaacatcaac cccggtggca gtatgctcgt
     3901 tgtttttcga ggattctgtc aggacgctgg gcactctata agcaagatcc ctttggacgt
     3961 catgaaaagt ctgggagggt atgatcctgc gaatgtgatc aggaaaaaaa gaccatcaaa
     4021 gaaggctgct tagtttgtt
//
LOCUS       DAZOZD010000763         3428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.349, whole genome shotgun sequence.
ACCESSION   DAZOZD010000763 DAZOZD010000000
VERSION     DAZOZD010000763.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.349"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            85..558
                     /gene="dfrA1"
                     /locus_tag="ACHUPB_005069"
     CDS             85..558
                     /gene="dfrA1"
                     /locus_tag="ACHUPB_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR0761575.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            653..1177
                     /gene="sat2"
                     /locus_tag="ACHUPB_005070"
     CDS             653..1177
                     /gene="sat2"
                     /locus_tag="ACHUPB_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR0761576.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1246..1743
                     /locus_tag="ACHUPB_005071"
     CDS             1246..1743
                     /locus_tag="ACHUPB_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761577.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            1804..2175
                     /locus_tag="ACHUPB_005072"
     CDS             1804..2175
                     /locus_tag="ACHUPB_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761578.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            2585..2962
                     /locus_tag="ACHUPB_005073"
     CDS             2585..2962
                     /locus_tag="ACHUPB_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0761579.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            2985..3137
                     /locus_tag="ACHUPB_005074"
                     /pseudo
     CDS             2985..3137
                     /locus_tag="ACHUPB_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(3193..>3428)
                     /locus_tag="ACHUPB_005075"
     CDS             complement(3193..>3428)
                     /locus_tag="ACHUPB_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR0761580.1"
                     /translation="TKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGH
                     RGVNHPKGLGELQVSREDMEGWAERVVREQFTH"
BASE COUNT          946 a          817 c          800 g          865 t
ORIGIN      
        1 tgattatatt aattaacggt aagcatcagc gggtgacaaa acgagcatgc ttactaataa
       61 aatgttaacc tctgaggaag aattgtgaaa ctatcactaa tggtagctat atcgaagaat
      121 ggagttatcg ggaatggccc tgatattcca tggagtgcca aaggtgaaca gctcctgttt
      181 aaagctatta cctataacca atggctgttg gttggacgca agacttttga atcaatggga
      241 gcattaccca accgaaagta tgcggtcgta acacgttcaa gttttacatc tgacaatgag
      301 aacgtattga tctttccatc aattaaagat gctttaacca acctaaagaa aataacggat
      361 catgtcattg tttcaggtgg tggggagata tacaaaagcc tgatcgatca agtagataca
      421 ctacatatat ctagaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc
      481 agcaatttta ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa
      541 atctggcaaa agggttaaca agtggcagca acggattcgc aaacctgtca cgccttttgt
      601 accaaaagcc gcgccaggtt tgcgatccgc tgtgccaggc gttaggcgtc atatgaagat
      661 ttcggtgatc cctgagcagg tggcggaaac attggatgct gagaaccatt tcattgttcg
      721 tgaagtgttc gatgtgcacc tatccgacca aggctttgaa ctatctacca gaagtgtgag
      781 cccctaccgg aaggattaca tctcggatga tgactctgat gaagactctg cttgctatgg
      841 cgcattcatc gaccaagagc ttgtcgggaa gattgaactc aactcaacat ggaacgatct
      901 agcctctatc gaacacattg ttgtgtcgca cacgcaccga ggcaaaggag tcgcgcacag
      961 tctcatcgaa tttgcgaaaa agtgggcact aagcagacag ctccttggca tacgattaga
     1021 gacacaaacg aacaatgtac ctgcctgcaa tttgtacgca aaatgtggct ttactctcgg
     1081 cggcattgac ctgttcacgt ataaaactag acctcaagtc tcgaacgaaa cagcgatgta
     1141 ctggtactgg ttctcgggag cacaggatga cgcctaacaa ttcattcaag ccgacaccgc
     1201 ttcgcggcgc ggcttaattc aggagttaga gagctgggga agactatgcg cgatctgttg
     1261 aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca
     1321 attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt
     1381 tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac
     1441 aactctgaga gcaactacga taatagttca tccaattacg acaatagtcg caacggaaat
     1501 cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc
     1561 aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa
     1621 ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata
     1681 aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac
     1741 tagcctgctc tctaataaaa tgttaggcct caacatctag tcgcaagctg aggggaacca
     1801 ctagtgtcat acgaacctcc aagagacggt tacacaaacg ggtacattgt tgatgtcatg
     1861 tatgacaatc gcccaagtaa gtatccagct gtgttcagaa cgtacgtccg aattccagac
     1921 tatgtcgaac tagataacgc ggaggagctg gccgacgcaa tcattcgaga ggttgagtta
     1981 aaggaggcat ggctcacaag ggtcttgggg aactcagccg atcctgacgc ttctgtgggc
     2041 aagtgggacc ggcgcctagc gcagtgcggt ggtattgatg agatgtcaag tggggtgcag
     2101 gcaacatact tcagtccaaa cggtcagctg gtcggcttag tggcatacat tgatgccgaa
     2161 tggatggatg aatagctctg acgttacgat tcaggtgaga caaccagatg ggcattgcgt
     2221 acaagctcag ttgccaccga tgtggctacg agtctgatct tctttaccta ggccaaggta
     2281 tggcgatgct tcctgagcaa atagtcggaa cgtgcacatg ctgcgacaat ctaacgacga
     2341 tttctataaa tgaaagctac ggagtatgct cactttgtgg ggctaaggat cgtgtgatat
     2401 ttacaagcta tcagcatgag gcaaggccaa gaaatccctg ctatctagag agaagtttca
     2461 aatatcaatg cccaagatgc catgccttca gtatggagcc tccggatcta ccggagatgc
     2521 tttgggacta atgctccgct ttgcctcggg tatccgttcg acctatgttt aagaggaaac
     2581 gcacgtgtct gccgccaaag ctcttgccta tgcactttgt gacaagttgg aaagcaaaaa
     2641 agttgatgaa gtcgtacgaa atcttgtttc tagcggctgg aatattaggg aagtggcctg
     2701 ggaagcttcg tcctttgatc atgaaatgcc ctactttgac acggggcggg acttcgatat
     2761 tggatttcct gacacgctgt catccaagat tgagtttttg accgcgaatg gatcaatcgt
     2821 ccaagcacgt gctcgcctgc acttcaagca ggctctaatt tttgccaagg cgtccaaaca
     2881 tgtcgccgcg ctcaagaact cattggatta ctactatgga tcgtctgtaa tcccccctca
     2941 aaaccacgga ggctatttgt agaaaattcc attaatatga actcaaggtg ttccctacgg
     3001 acgaatcagc gatgaaggtg gtgtacctgg ctatccaggc ggcgaccaag aaatggacga
     3061 tgccgatccg caactggaag ccggccatga accgttttat gatcgaattt ggtgaccgtt
     3121 taaatggcca cctttaactg accggcaaaa gcacttacac aaaagggttt acaggctctt
     3181 tcagtcattc ctttaatgcg taaattgctc tctaaccact ctctcagccc acccttccat
     3241 atcctctctc gatacttgca gctcccccaa cccctttgga tgattcaccc ctctatgccc
     3301 gtcttgtcca tataattgat caaacaaact atttggccaa ttcaatgagc tcttcaccac
     3361 tccacgccgt atcttttcaa aatcattccg ccatgtttcg ctatcaacgc cactcaacac
     3421 tttggtag
//
LOCUS       DAZOZD010000764         2449 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.407, whole genome shotgun sequence.
ACCESSION   DAZOZD010000764 DAZOZD010000000
VERSION     DAZOZD010000764.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.407"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(187..537)
                     /locus_tag="ACHUPB_005076"
                     /pseudo
     CDS             complement(187..537)
                     /locus_tag="ACHUPB_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(740..1753)
                     /gene="intI1"
                     /locus_tag="ACHUPB_005077"
     CDS             complement(740..1753)
                     /gene="intI1"
                     /locus_tag="ACHUPB_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR0761581.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1911..2384
                     /gene="dfrA17"
                     /locus_tag="ACHUPB_005078"
     CDS             1911..2384
                     /gene="dfrA17"
                     /locus_tag="ACHUPB_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR0761582.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
BASE COUNT          582 a          666 c          662 g          539 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc cccaatgcgc tgtccagtca
       61 tggaattccc tgcaaaatgt cagggaagac tctatgacct tcaacgagat atgtcaataa
      121 attcaaaatt caatcctatc ctgacgcaat ttacacatgg catctgacat caggttaggg
      181 tatgcctcaa cctgacggcg gcgaatcaca agcgtccggt ttgacgctgg tttcggtcgc
      241 agtgctggcc cgcgcctgga agcgctgata gcactccagc ccgcagaaat gctccacgta
      301 ctcggcccct tccggcgtga aggcggcatc gagcgggatt tccttgcagc acacgcagca
      361 actggtgctc ggttcattgg cgttcatggt ggtgttcctc cattggttga cgaagccgac
      421 gaaggccgcc gccggcatcg gcctggcgaa caggaaaccc tgcaccgtgt cgcaacccgc
      481 ctgccgcaac cacgcaaggc agtcgggtgt ttccacaccc tcggctacca cctccattgt
      541 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
      601 gttggatcca tcaggcaacg acgggctgct gccggccatc agcggacgca gggaggactt
      661 tccgcaaccg gccgttcgat gcggcaccga tggccttcgc gcaggggtag tgaatccgcc
      721 aggattgact tgcgctgccc tacctctcac tagtgagggg cggcagcgca tcaagcggtg
      781 agcgcactcc ggcaccgcca actttcagca catgcgtgta aatcatcgtc gtagagacgt
      841 cggaatggcc gagcagatcc tgcacggttc gaatgtcgta accgctgcgg agcaaggccg
      901 tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt cgtgatgcct gcttgttcta
      961 cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat gtgatggcga cgcacgacac
     1021 cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac ccagaaccac ggccaggaat
     1081 gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg ctgcggccct
     1141 cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc aggctgggtg
     1201 ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc cgcacgatga
     1261 tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca ctgatccgca
     1321 tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga aaaccgagga
     1381 tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc
     1441 cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg
     1501 ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
     1561 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
     1621 tgaaggcacg aacccagtgg acataagcct gttcggttcg taaactgtaa tgcaagtagc
     1681 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1741 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
     1801 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1861 cgcagcaggg cagtcgccct aaaacaaagt tagccattaa gggagttaaa ttgaaaatat
     1921 cattgatttc tgcagtgtca gaaaatggcg taatcggtag tggtcctgat atcccgtggt
     1981 cagtaaaagg tgagcaacta ctctttaaag cgctcacata taatcaatgg ctccttgtcg
     2041 gaagaaaaac atttgactct atgggtgttc ttccaaatcg caaatatgca gtagtgtcaa
     2101 agaacggaat ttcaagctca aatgaaaacg tcctagtttt tccttcaata gaaaatgctt
     2161 tgaaagagct atcaaaagtt acagatcatg tatatgtctc tggcgggggt caaatctata
     2221 atagccttat tgaaaaagca gatataattc atttgtctac tgttcacgtt gaagtcgaag
     2281 gtgatatcaa attccctata atgcctgaga atttcaattt ggtttttgaa cagtttttta
     2341 tgtctaatat aaattataca taccagattt ggaaaaaagg ctaacaatgc gttgcagcac
     2401 cagtcgcttc gctccttgga cagcttttaa gtcgcgtctt tgtggtttt
//
LOCUS       DAZOZD010000765         1891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.450, whole genome shotgun sequence.
ACCESSION   DAZOZD010000765 DAZOZD010000000
VERSION     DAZOZD010000765.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.450"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            complement(<1..315)
                     /locus_tag="ACHUPB_005079"
     CDS             complement(<1..315)
                     /locus_tag="ACHUPB_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006939175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR0761583.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFY"
     gene            381..812
                     /locus_tag="ACHUPB_005080"
                     /pseudo
     CDS             381..812
                     /locus_tag="ACHUPB_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            995..1855
                     /locus_tag="ACHUPB_005081"
     CDS             995..1855
                     /locus_tag="ACHUPB_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR0761584.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          496 a          441 c          494 g          460 t
ORIGIN      
        1 ataaaaatcg acagtgcggc cccggctgtc gacggcccgg tacagatacg cccagcggcc
       61 attgaccttc acgtaggttt catccatgtg ccacgggcaa agatcggaag ggttacgcca
      121 gtaccagcgc agccgttttt ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt
      181 ggagtgatcg acattcactc cgcgttcagc cagcatctcc tgcagctcac ggtaactgat
      241 gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc
      301 tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg
      361 actatttgca acagtgccca acagaccggg aagggctgga tttgctgagg atgaaggtgg
      421 aggaaggtga tgtcattctg gttaagaagc tcgaccgtct tggccgcgac actgccgata
      481 tgatccaact gataaaggaa tttgacgctc agggcgtggc agtccggttc attgatgacg
      541 ggatcagtac cgacggtgat atggggcaaa tggtggtcac catcctgtcg gctgtggcac
      601 aggctgaacg ccggaggatc ctagaacgca cgaatgaggg ccgacaggaa gcaaagctga
      661 aaggaatcaa atttggccgc aggcgtaccg tggacaggaa cgtcgtgctg acgcttcatc
      721 agaagggcac tggtgcaacg gaaattgctc atcagctcag tattgcccgc tccacggttt
      781 ataaaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      841 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      961 gtaaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt ttcgtgtcgc
     1021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1201 ttttaaagtt ctgctatgtg gtgcggtatt atcccgtgtt gacgccgggc aagagcaact
     1261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1381 taacactgct gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1501 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     1561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1861 agaccaagtt tactcatata tactttagat t
//
LOCUS       DAZOZD010000766         1830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.457, whole genome shotgun sequence.
ACCESSION   DAZOZD010000766 DAZOZD010000000
VERSION     DAZOZD010000766.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.457"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            110..1015
                     /locus_tag="ACHUPB_005082"
     CDS             110..1015
                     /locus_tag="ACHUPB_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR0761585.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..>1830
                     /gene="mrx(A)"
                     /locus_tag="ACHUPB_005083"
                     /gene_synonym="mrx"
     CDS             1012..>1830
                     /gene="mrx(A)"
                     /locus_tag="ACHUPB_005083"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR0761586.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHA"
BASE COUNT          280 a          587 c          599 g          364 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg
//
LOCUS       DAZOZD010000767          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1908091
            SAMN43277405-rid23486453.guided.616, whole genome shotgun sequence.
ACCESSION   DAZOZD010000767 DAZOZD010000000
VERSION     DAZOZD010000767.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277405
            Sequence Read Archive: SRR30464421
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396772.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396772.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 06:12:12
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,994
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 147 of 342
            Pseudo Genes (incomplete)         :: 138 of 342
            Pseudo Genes (internal stop)      :: 112 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277405-rid23486453.guided.616"
                     /strain="M1908091"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2019-03-30"
                     /collected_by="FSS"
     gene            <1..685
                     /gene="erm"
                     /locus_tag="ACHUPB_005084"
     CDS             <1..685
                     /gene="erm"
                     /locus_tag="ACHUPB_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFR0761587.1"
                     /translation="NQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSH
                     LFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHAS
                     DIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRH
                     TTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFN
                     SYLLFNGRK"
     gene            630..>748
                     /locus_tag="ACHUPB_005085"
     CDS             630..>748
                     /locus_tag="ACHUPB_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HFR0761588.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDK"
BASE COUNT          273 a          131 c          122 g          222 t
ORIGIN      
        1 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac
       61 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga
      121 attagacagt catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac
      181 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt
      241 tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt ttgaaagcca
      301 tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct tggatattca
      361 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc
      421 agcggaatgc tttcatccta aaccaagagt aaacagtgtc ttaataaaac ttacccgcca
      481 taccacagat gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt
      541 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc
      601 caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta
      661 tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac
      721 acgttactaa agggaatgta gataaatt
//
